INEB
INEB
TitleAutomatic lane and band detection in images of thin layer chromatography
Publication TypeBook
Year of Publication2004
AuthorsSousa, AV, Aguiar, R, Mendonca, AM, Campilho, A
Series TitleLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.
Volume3212
Number of Pages158 - 165
ISBN Number03029743 (ISSN)
AbstractThis work aims at developing an automatic method for the analysis of TLC images for measuring a set of features that can be used for the characterization of the distinctive patterns that result from the separation of oligosaccharides contained in human urine. This paper describes the methods developed for the automatic detection of the lanes contained in TLC images, and for the automatic separation of bands for each detected lane. The extraction of quantitative information related with each band was accomplished with two methods: the EM expectation-maximization and nonlinear least squares trustregion algorithms. The results of these methods, as well as additional quantitative information related with each band, are also presented. © Springer-Verlag 2004.
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