| Title | Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth |
| Publication Type | Journal Article |
| 2010 |
| Authors | Mittnacht, U, Hartmann, H, Hein, S, Oliveira, H, Dong, M, Pêgo, AP, Kjems, J, Howard, KA, Schlosshauer, B |
| Journal | Nano LettersNano Lett. |
| Volume | 10 |
| Issue | 10 |
| Pagination | 3933 - 3939 |
| Date Published | 2010/// |
| 15306984 (ISSN) |
| animal, Animals, article, Cell Line, cell viability, chemistry, Chitin, chitosan, cytology, Drug delivery, genetic transfection, genetics, Local delivery, metabolism, Microstructured, nanobiofunctionalized implants, nanoparticle, nanoparticles, nerve cell, nerve regeneration, Nerve repair, neurite, Neurite outgrowth, Neurites, Neurons, Polymer filaments, Prostheses and Implants, prostheses and orthoses, rhoA GTP-Binding Protein, RhoA guanine nucleotide binding protein, RNA, RNA, Small Interfering, siRNA, small interfering RNA, Spinal cords, Transfection, ultrastructure |
| Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair. © 2010 American Chemical Society. |
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