| Title | Direct and indirect effects of P 2O
5 glass reinforced-hydroxyapatite composites on the growth and function of osteoblast-like cells
|
| Publication Type | Journal Article |
| 2000 |
| Authors | Lopes, MA, Knowles, JC, Santos, JD, Monteiro, FJ, Olsen, I |
| Journal | BiomaterialsBiomaterials |
| Volume | 21 |
| Issue | 11United Kingdom |
| Pagination | 1165 - 1172 |
| Date Published | 2000/// |
| 01429612 (ISSN) |
| Animal cell culture, antigen expression, Antigens, article, Biocompatibility, Biocompatible Materials, Biomaterials, Bone, Bone cells, cell antigen, Cell culture, Cell Division, cell function, cell growth, Cell Line, cell proliferation, cell structure, collagen type 1, controlled study, DNA, DNA Replication, DNA synthesis, extracellular matrix, Fibronectin, flow cytometry, glass, Glass fibers, Glass-reinforced hydroxyapatite, Growth kinetics, human, human cell, Humans, hydroxyapatite, Hydroxyapatites, immunofluorescence, In vitro, Microscopy, Electron, Scanning, osteoblast, Osteoblasts, osteocalcin, Phosphate minerals, Phosphates, phosphorus pentoxide, priority journal, Proteins, Reinforcement, Scanning electron microscopy |
| Human osteoblast-like cells were plated on hydroxyapatite and P 2O
5-glass reinforced hydroxyapatite composite discs. They were also cultured in the presence of media obtained by incubating the discs in the absence of cells. The effects of these direct and indirect interactions were examined by measuring cell proliferation and the expression of certain key extracellular matrix antigens. One composite was found to initially delay cell growth, while the extract of a different composite appeared to down-regulate DNA synthesis. Flow cytometry analysis showed that growth directly on the discs had little effect on collagen type I, but reduced fibronectin and osteocalcin levels. The extracts of the materials generally had less effect, although one extract obtained from the glass-reinforced hydroxyapatite significantly down-regulated fibronectin. These in vitro studies thus suggest that there were only few differences overall in the growth of the cells directly on the glass-reinforced compared with the hydroxyapatite discs and also only relatively small effects of the extracts on the cells. However, the flow cytometry results suggest that both the materials and the extracts may have a potentially important influence on connective tissue production, and that these effects are both material- and antigen-specific. Copyright (C) 2000 Elsevier Science Ltd.
Human osteoblast-like cells were plated on hydroxyapatite and P
2O
5-glass reinforced hydroxyapatite composite discs. They were also cultured in the presence of media obtained by incubating the discs in the absence of cells. The effects of these direct and indirect interactions were examined by measuring cell proliferation and the expression of certain key extracellular matrix antigens. One composite was found to initially delay cell growth, while the extract of a different composite appeared to down-regulate DNA synthesis. Flow cytometry analysis showed that growth directly on the discs had little effect on collagen type I, but reduced fibronectin and osteocalcin levels. The extracts of the materials generally had less effect, although one extract obtained from the glass-reinforced hydroxyapatite significantly down-regulated fibronectin. These in vitro studies thus suggest that there were only few differences overall in the growth of the cells directly on the glass-reinforced compared with the hydroxyapatite discs and also only relatively small effects of the extracts on the cells. However, the flow cytometry results suggest that both the materials and the extracts may have a potentially important influence on connective tissue production, and that these effects are both material- and antigen-specific.
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