| Title | Dynamic cell-cell interactions between cord blood haematopoietic progenitors and the cellular niche are essential for the expansion of CD34 +, CD34
+CD38
|
| Publication Type | Journal Article |
| 2010 |
| Authors | Da Silva, CL, Gonçalves, R, Dos Santos, F, Andrade, PZ, Almeida-Porada, G, Cabral, JMS |
| Journal | Journal of Tissue Engineering and Regenerative MedicineJ. Tissue Eng. Regenerative Med. |
| Volume | 4 |
| Issue | 2 |
| Pagination | 149 - 158 |
| Date Published | 2010/// |
| 19326254 (ISSN) |
| Antigens, CD34, Antigens, CD38, Antigens, CD7, article, Bone marrow microenvironment, CD34 antigen, CD38 antigen, CD7 antigen, cell adhesion, Cell Communication, cell contact, Cell culture, cell differentiation, cell interaction, cell proliferation, Cells, Cultured, cellular distribution, clonogenic assay, coculture, Coculture Techniques, Colony-Forming Units Assay, controlled study, Ex vivo expansion, ex vivo study, Fetal Blood, flow cytometry, Haematopoietic stem/progenitor cells, hematopoietic stem cell, Hematopoietic Stem Cells, human, human cell, Humans, Lymphocytes, lymphoid cell, Mesenchymal stem cell, Mesenchymal Stem Cells, Microscopy, Electron, Scanning, phenotype, priority journal, Scanning electron microscopy, somatic gene therapy, stem cell expansion, Stem Cell Niche, stroma cell, Suspensions, umbilical cord blood |
| Most clinical applications of haematopoietic stem/progenitor cells (HSCs) would benefit from their ex vivo expansion to obtain a therapeutically significant amount of cells from the available donor samples. We studied the impact of cellular interactions between umbilical cord blood (UCB) haematopoietic cells and bone marrow (BM)-derived mesenchymal stem cells (MSCs) on the ex vivo expansion and differentiative potential of UCB CD34 +-enriched cells. UCB cells were cultured: (a) directly in contact with BM MSC-derived stromal layers (contact); (b) separated by a microporous membrane (non-contact); or (c) without stroma (no stroma). Highly dynamic culture events occurred in HSC-MSC co-cultures, involving cell-cell interactions, which preceded HSC expansion. Throughout the time in culture [18 days], total cell expansion was significantly higher in contact (fold increase of 280 ± 37 at day 18) compared to non-contact (85 ± 25). No significant cell expansion was observed in stroma-free cultures. CD34
+ cell expansion was also clearly favoured by direct contact with BM MSCs (35 ± 5- and 7 ± 3-fold increases at day 18 for contact and non-contact, respectively). Moreover, a higher percentage of CD34
+CD38
|
| http://www.scopus.com/inward/record.url?eid=2-s2.0-77649221153&partnerID=40&md5=822edb72ad71976fb309b0e658137bc5 |
