| Title | Effects of Co-Cr corrosion products and corresponding separate metal ions on human osteoblast-like cell cultures |
| Publication Type | Journal Article |
| 1996 |
| Authors | Tomás, H, Carvalho, GS, Fernandes, MH, Freire, AP, Abrantes, LM |
| Journal | Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED. |
| Volume | 7 |
| Issue | 5London, United Kingdom |
| Pagination | 291 - 296 |
| Date Published | 1996/// |
| 09574530 (ISSN) |
| Absorption spectroscopy, article, Atomic spectroscopy, Biocompatibility, Bone, Cell culture, Chromium alloys, Cobalt alloys, Corrosion, Corrosion resistance, Cytocompatibility, Enzyme inhibition, human, human cell, Human osteoblasts, Implants (surgical), Orthopaedic alloys, ossification, osteoblast, priority journal, surface property |
| The cytocompatibility of the degradation products of a Co-Cr orthopaedic alloy was investigated with particular focus on the dose-effect of an electrochemically dissolved alloy extract and of the corresponding separate metal ions on human osteogenic bone marrow derived cells. The extract solution contained 15ppm of Co and 8ppm of Cr as analysed by atomic absorption spectroscopy. Stock salt solutions of CoCl 2·6H
2O, CrCl
3·6H
2O and Na
2CrO
4 at corresponding concentrations were also prepared. Several dilutions of the above metallic solutions were tested for a period of 21 days on cells (third subculture) cultured in α-minimal essential medium containing foetal bovine serum and supplemented with antibiotics, dexamethasone ascorbic acid and β-glycerophosphate. The osteoblast response to the presence of metal ions was evaluated by several biochemical parameters: cell viability (MTT reduction by intracellular enzymes), alkaline phosphatase activity (an osteoblast marker) and protein production (both intracellular and extracellular). Co-Cr corrosion products showed opposite effects to their respective metal salts only on day 1. With time the different metal solutions presented a similar pattern of inhibition. These results suggest that impaired bone formation in vitro can occur in the presence of Co-Cr corrosion products.
The cytocompatibility of the degradation products of a Co-Cr orthopaedic alloy was investigated with particular focus on the dose-effect of an electrochemically dissolved alloy extract and of the corresponding separate metal ions on human osteogenic bone marrow derived cells. The extract solution contained 15 ppm of Co and 8 ppm of Cr as analyzed by atomic absorption spectroscopy. Stock salt solutions of COCl
2·6H
2O, CrCl
3·6H
2O and Na
2CrO
4 at corresponding concentrations were also prepared. Several dilutions of the above metallic solutions were tested for a period of 21 days on cells (third subculture) cultured in α-minimal essential medium containing foetal bovine serum and supplemented with antibiotics, dexamethasone ascorbic acid and β-glycerophosphate. The osteoblast response to the presence of metal ions was evaluated by several biochemical parameters: cell viability (MTT reduction by intracellular enzymes), alkaline phosphatase activity (an osteoblast marker) and protein production (both intracellular and extracellular). Co-Cr corrosion products showed opposite effects to their respective metal salts only on day 1. With time the different metal solutions presented a similar pattern of inhibition. These results suggest that impaired bone formation in vitro can occur in the presence of Co-Cr corrosion products.
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