Title | Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII 7-10)
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Publication Type | Miscellaneous |
Year of Publication | 2012 |
Authors | Amaral, IF, Neiva, I, Ferreira da Silva, F, Sousa, SR, Piloto, AM, Lopes, CDF, Barbosa, MA, Kirkpatrick, CJ, Pêgo, AP |
ISBN Number | 17427061 (ISSN) |
Keywords | Protein conformation, Protein radiolabelling, Spinal cord injury, Surface grafting, Three-dimensional scaffolds |
Abstract | The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4% and 15%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII 7-10). Immobilized rhFNIII
7-10 was characterized in terms of amount (
125I-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII
7-10 with rhFNIII
7-10 concentration, and, for the same concentration, higher amounts of rhFNIII
7-10 on DA 4% compared with DA 15%. Moreover, rhFNIII
7-10 concentrations as low as 5 and 20 μg ml
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URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84868680582&partnerID=40&md5=6599fd2565f4511b94e944978afc8073 |