



@article {2269,
	title = {Heparinized hydroxyapatite/collagen three-dimensional scaffolds for tissue engineering},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {21},
	year = {2010},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-010-4097-2PubMed ID: 20596760Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: collagen, 9007-34-5; cyanamide, 151-51-9, 420-04-2; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; hydroxyapatite, 1306-06-5, 51198-94-8; n hydroxysuccinimide, 6066-82-6; trinitrobenzenesulfonic acid, 16655-63-3, 2508-19-2; Biocompatible Materials; Bone Substitutes; Collagen, 9007-34-5; Cross-Linking Reagents; Durapatite, 1306-06-5; Heparin, 9005-49-6; PolymersReferences: Rose, F.R., Cyster, L.A., Grant, D.M., Scotchford, C.A., Howdle, S.M., Shakesheff, K.M., In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel (2004) Biomaterials, 25 (24), pp. 5507-5514. , DOI 10.1016/j.biomaterials.2004.01.012, PII S0142961204000134;Hong, Z., Zhang, P., He, C., Qiu, X., Liu, A., Chen, L., Chen, X., Jing, X., Nano-composite of poly(L-lactide) and surface grafted hydroxyapatite: Mechanical properties and biocompatibility (2005) Biomaterials, 26 (32), pp. 6296-6304. , DOI 10.1016/j.biomaterials.2005.04.018, PII S0142961205003297; 
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},
	month = {2010///},
	pages = {2385 - 2392},
	abstract = {Currently, in bone tissue engineering research, the development of appropriate biomaterials for the regeneration of bony tissues is a major concern. Bone tissue is composed of a structural protein, collagen type I, on which calcium phosphate crystals are enclosed. For tissue engineering, one of the most applied strategies consists on the development and application of three dimensional porous scaffolds with similar composition to the bone. In this way, they can provide a physical support for cell attachment, proliferation, nutrient transport and new bone tissue infiltration. Hydroxyapatite is a calcium phosphate with a similar composition of bone and widely applied in several medical/dentistry fields. Therefore, in this study, hydroxyapatite three dimensional porous scaffolds were produced using the polymer replication method. Next, the porous scaffolds were homogeneously coated with a film of collagen type I by applying vacuum force. Yet, due to collagen degradability properties, it was necessary to perform an adequate crosslinking method. As a result, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was employed as an efficient and non-toxic crosslinking method in this research. The composites were characterized by means of SEM, DSC and TNBS. Furthermore, heparin was incorporated in order to accomplish sustained delivery of a growth factor of interest namely, bone morphogenetic proteins (BMP-2). BMP-2 binding and release of non-heparinized and heparinized scaffolds was evaluated at specific time points. The incorporation of heparin leads to a reduced initial burst phase when compared to the non heparinized materials. The results show a beneficial effect with the incorporation of heparin and its potential as a localized drug delivery system for the sustained release of growth factors. {\textcopyright} 2010 Springer Science+Business Media, LLC.},
	keywords = {Achilles Tendon, Animals, Apatite, article, Beneficial effects, Binding and release, Biocompatible Materials, Biological materials, Biomaterials, Bone, Bone and Bones, bone morphogenetic protein 2, Bone morphogenetic proteins, Bone Substitutes, bone tissue, Bone tissue engineering, Bony tissue, Calcium, calcium phosphate, Calcium phosphate crystals, Cattle, Cell attachments, Collagen, collagen degradation, collagen type 1, Collagen type I, controlled study, cross linking, Cross-Linking Reagents, Crosslinking, cyanamide, Degradability, differential scanning calorimetry, Drug delivery, drug delivery system, Durapatite, Grafts, Growth factor, heparin, Hydrogels, hydroxyapatite, Microscopy, Electron, Scanning, n (3 dimethyllaminopropyl) n{\textquoteright} ethylcarbodiimide, n hydroxysuccinimide, N-hydroxysuccinimide, Nutrient transport, Peptides, polymer, Polymer replication, Polymers, Polysaccharides, porosity, Porous scaffold, priority journal, protein binding, Scaffolds, Scanning electron microscopy, SEM, Specific time, Structural proteins, Sustained release, Three dimensional, Three-dimensional porous scaffolds, Three-dimensional scaffolds, Tissue engineering, tissue scaffold, Tissue Scaffolds, trinitrobenzenesulfonic acid, unclassified drug, vacuum, Vacuum force},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956420058\&partnerID=40\&md5=a723fdb7ea856a5286080a1a4aab82a2},
	author = {Teixeira, S. and Yang, L. and Dijkstra, P.J. and Ferraz, M.P. and Monteiro, F.J.}
}
