



@book {2295,
	title = {Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5627 LNCS},
	year = {2009},
	note = {Conference code: 77746Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02611-9_81Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica Divis{\~a}o de Sinal e Imagem, Campus FEUPReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, p. 13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
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Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
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Schmitt, M.H.O., Morphological multiscale decomposition of connected regions with emphasis on cell clusters (2009) Computer Vision and Image Understanding, 113, pp. 188-201; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. on Circuits and Systems I-Regul. Papers, 53 (11), pp. 2405-2414; 
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Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proceedings of the Int. Conference on Image Analysis and Processing, pp. 757-761; 
},
	month = {2009///},
	pages = {824 - 831},
	address = {Halifax, NS},
	abstract = {The study of cell nuclei is fundamental for plant cell Biology research. To obtain information at cellular level, researchers image cells{\textquoteright} nuclei which were modified with fluorescence proteins, through laser scanning confocal microscopy. These images are normally noisy and suffer from high background fluorescence, making grey-scale segmentation approaches inadequate for a usable detection. To obtain a successful detection even at low contrast we investigate the use of a particular convergence filter, the Symmetric Sliding Band filter (SSBF), for cell detection. This filter is based on gradient convergence and not intensity. As such it can detect low contrast cell nuclei which otherwise would be lost in the background noise. Due to the characteristics of cell nuclei morphology, a symmetry constrain is integrated in the filter which corrects some inadequate detections and results in a filter response that is more discriminative. We evaluate the use of this filter for cell nuclei detection on the Arabidopsis thaliana root tip, where the nuclei were stained using yellow fluorescence protein. The resulting cell nuclei detection precision is 89\%. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {Arabidopsis thaliana, Background noise, Band filters, Bioinformatics, Biology imaging, Cell detection, Cell membranes, cell nucleus, Cellular levels, confocal microscopy, Convergence filters, cytology, Filter response, Fluorescence, Fluorescence proteins, Fluorescent images, Image analysis, Laser scanning confocal microscopy, Low contrast, Plant cell culture, Plant cells, Positron emission tomography, Root tip, Yellow fluorescence proteins},
	isbn = {03029743 (ISSN); 3642026109 (ISBN); 9783642026102 (ISBN)},
	author = {Marcuzzo, M. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
