



@article {2408,
	title = {Leptin effect on RANKL and OPG expression in MC3T3-E1 osteoblasts},
	journal = {Journal of Cellular BiochemistryJ. Cell. Biochem.},
	volume = {98},
	year = {2006},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: ScopusCODEN: JCEBDdoi: 10.1002/jcb.20853PubMed ID: 16479591Language of Original Document: EnglishCorrespondence Address: Lamghari, M.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180, Porto, Portugal; email: lamghari@ineb.up.ptChemicals/CAS: osteoclast differentiation factor, 200145-93-3; osteoprotegerin, 205944-50-9; Alkaline Phosphatase, EC 3.1.3.1; Carrier Proteins; Glycoproteins; leptin receptor; Leptin; Membrane Glycoproteins; Osteoprotegerin; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Receptors, Cell Surface; Receptors, Cytoplasmic and Nuclear; Receptors, Tumor Necrosis Factor; RNA, Messenger; Tnfrsf11a protein, mouse; Tnfrsf11b protein, mouse; Tnfsf11 protein, mouseReferences: Burguera, B., Hofbauer, L.C., Thomas, T., Gori, F., Evans, G.L., Khosla, S., Riggs, B.L., Turner, R.T., Leptin reduces ovariectomy-induced bone loss in rats (2001) Endocrinology, 142, pp. 3546-3553;Cornish, J., Callon, K.E., Bava, U., Lin, C., Naot, D., Hill, B.L., Grey, A.B., Reid, I.R., Leptin directly regulates bone cell function in vitro and reduces bone fragility in vivo (2002) J Endocrinol, 175, pp. 405-415; 
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},
	month = {2006///},
	pages = {1123 - 1129},
	abstract = {Recent studies have suggested that leptin hormone may play a pivotal role on bone remodeling through a direct effect by modulating positively the OPG/RANKL balance. Here, we investigate the effect of leptin hormone on RANKL and OPG expression in MC3T3-E1 osteoblasts using RT-PCR and ELISA measurements. We have at first identified the expression of Ob-Rb and Ob-Ra leptin receptor isoforms in MC3T3-E1 and observed that these cells respond to mrleptin treatments. We then investigated the effect of mrleptin on RANKL and OPG expression. We show that mrleptin dose-dependently regulated the expression of RANKL mRNA with complete inhibition observed at concentrations higher than 12 ng/ml. This effect was confirmed with sRANKL protein measurements. However, the exposure of MC3T3-E1 to mrleptin had no effect on OPG mRNA. Taken together, these results suggest that leptin modulates positively OPG/RANKL balance by inhibiting the expression of RANKL gene. {\textcopyright} 2006 Wiley-Liss, Inc.},
	keywords = {alkaline phosphatase, animal cell, Animals, article, bone remodeling, Carrier Proteins, Cell Line, enzyme linked immunosorbent assay, gene expression, gene expression regulation, Glycoproteins, hormone action, leptin, leptin receptor, leptin receptor a, leptin receptor b, MC3T3-E1, Membrane Glycoproteins, messenger RNA, Mice, mouse, nonhuman, Ob-Ra, Ob-Rb, OPG, osteoblast, Osteoblasts, osteoclast differentiation factor, Osteoprotegerin, priority journal, protein expression, RANK Ligand, RANKL, Receptor Activator of Nuclear Factor-kappa B, Receptors, Cell Surface, Receptors, Cytoplasmic and Nuclear, Receptors, Tumor Necrosis Factor, reverse transcription polymerase chain reaction, RNA extraction, RNA, Messenger, unclassified drug},
	isbn = {07302312 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33746452603\&partnerID=40\&md5=f5eb37e7159ced26f3b7e756d59bc1bb},
	author = {Lamghari, M. and Tavares, L. and Camboa, N. and Barbosa, M.A.}
}
