



@book {2428,
	title = {In vitro analysis of protein adhesion to phase pure hydroxyapatite and silicon substituted hydroxyapatite},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Botelho, C.M.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: ccunha@fe.up.ptReferences: Patel et al, J. of Mat. Sci.: Mat. in Med., 13 (2002) p. 1199Gibson, I.R., (1999) Bioceramics, 12, p. 191;Botelho, C.M., in Key (2003) Engineering Materials, 254-256, pp. 841-844; 
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Jha, L.J., Worldwide patent, PCT/GB97/02325 and US Patent Serial (1999), No 09/147773Gibson, I.R., (1999) J. Biomed. Mat. Res, 44, p. 422; 
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Reffit, D.M., (2003) Bone, 32, p. 127
},
	month = {2005///},
	pages = {461 - 464},
	abstract = {The adhesion of bovine collagen type I, bovine serum albumin, bovine IgG, 1 \% and 10 \% (v/v) human serum to hydroxyapatite (HA), silicon-substituted hydroxyapatite (Si-HA) and tissue culture plastic were studied. The materials were incubated at 37{\textdegree}C for 30 minutes, after which the protein solution was removed and analyzed. The adsorbed protein was evaluated by electrophoresis and immunoassay after extraction from the materials. The degree of adhesion was higher for collagen, followed by IgG and albumin on all materials. However there was no difference in the amount of collagen adsorbed onto the surface of each material and this was also the finding with albumin and IgG. These results suggest that the increased bioactivity seen with Si-HA is not due to the degree of protein adhesion, but may possibly be due to changes in the conformation of the bound proteins.},
	keywords = {Adsorption, Bioactivity, Bovine collagen type I, Bovine collagens, Bovine serum proteins, Collagen, Human serum proteins, hydroxyapatite, Immunoglobulin G (IgG), immunology, Silicon-substituted hydroxyapatite, Substitution reactions, Tissue culture},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-26844513571\&partnerID=40\&md5=ed134d3a2ccf1a8799bbb487ac863072},
	author = {Botelho, C.M. and Brooks, R.A. and Kawai, T. and Ogata, S. and Ohtsuki, C. and Best, S.M. and Lopes, M.A. and Santos, J.D. and Rushton, N. and Bonfield, W.}
}
