



@article {2460,
	title = {Proliferation, activity, and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {72},
	year = {2005},
	note = {Cited By (since 1996): 34Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30217PubMed ID: 15543603Language of Original Document: EnglishCorrespondence Address: Barrias, C.C.; INEB, Instituto de Engenharia Biomedica, Laboratorio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: ccbarrias@ineb.up.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; osteocalcin, 136461-80-8; titanium, 7440-32-6; Alkaline Phosphatase, EC 3.1.3.1; Calcium, 7440-70-2; Osteocalcin, 104982-03-8; titanium phosphate, 17017-57-1; Titanium, 7440-32-6References: Senuma, Y., Franceschin, S., Hilborn, J.G., Tissieres, P., Bisson, I., Frey, P., Bioresorbable microspheres by spinning disk atomization as injectable cell carrier: From preparation to in vitro evaluation (2000) Biomaterials, 21 (11), pp. 1135-1144;Hsu, F.Y., Chueh, S.C., Wang, Y.J., Microspheres of hydroxyapatite/ reconstituted collagen as supports for osteoblast cell growth (1999) Biomaterials, 20 (20), pp. 1931-1936; 
McGlohorn, J.B., Grimes, L.W., Webster, S.S., Burg, K.J., Characterization of cellular carriers for use in injectable tissue-engineering composites (2003) J Biomed Mater Res, 66 A (3), pp. 441-449; 
Wu, T.J., Huang, H.H., Lan, C.W., Lin, C.H., Hsu, F.Y., Wang, Y.J., Studies on the microspheres comprised of reconstituted collagen and hydroxyapatite (2004) Biomaterials, 25 (4), pp. 651-658; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Key Eng Mater, 254-256, pp. 877-880; 
Van Wezel, A.L., Microcarrier technology: Present status and prospects (1983) Dev Biol Stand, 55, pp. 3-9; 
Malda, J., Kreijveld, E., Temenoff, J.S., Van Blitterswijk, C.A., Riesle, J., Expansion of human nasal chondrocytes on macroporous microcarriers enhances redifferendation (2003) Biomaterials, 24 (28), pp. 5153-5161; 
Frondoza, C., Sohrabi, A., Hungerford, D., Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture (1996) Biomaterials, 17 (9), pp. 879-888; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., A novel route for the preparation of injectable ceramic porous microspheres for bone tissue engineering (2003) Ceramics, Cells and Tissues Annual Conferences: Bioceramic Surfaces Behavior in Vitro and in Vivo, pp. 228-232. , Ravaglioli A, Krajewski A, editors. Faenza: ISTEC-CNR editions; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., (2004) Preparation and Characterisation of Calcium-phosphate Microspheres for Biomedical Applications, , Submitted for publication; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254 (2), pp. 317-330; 
Labarca, C., Paigen, K., Simple, rapid, and sensitive DNA assay procedure (1980) Anal Biochem, 102 (2), pp. 344-352; 
Mankani, M.H., Kuznetsov, S.A., Fowler, B., Kingman, A., Robey, P.G., In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape (2001) Biotechnol Bioeng, 72 (1), pp. 96-107; 
Higashi, T., Okamoto, H., Influence of particle size of calcium phosphate ceramics as a capping agent on the formation of a hard tissue barrier in amputated dental pulp (1996) J Endod, 22 (6), pp. 281-283; 
Misiek, D.J., Kent, J.N., Carr, R.F., Soft tissue responses to hydroxylapatite particles of different shapes (1984) J Oral Maxillofac Surg, 42 (3), pp. 150-160; 
Maniatopoulos, C., Bone formation by young adult bone marrow stromal cells in vitro (1987) J Dent Res, 66, p. 104; 
Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Marshak, D.R., Multilineage potential of adult human mesenchymal stem cells (1999) Science, 284 (5411), pp. 143-147; 
Ter Brugge, P.J., Jansen, J.A., In vitro osteogenic differentiation of rat bone marrow cells subcultured with and without dexamethasone (2002) Tissue Eng, 8 (2), pp. 321-331; 
Anselme, K., Osteoblast adhesion on biomaterials (2000) Biomaterials, 21 (7), pp. 667-681; 
Anselme, K., Linez, P., Bigerelle, M., Le Maguer, D., Le Maguer, A., Hardouin, P., Hildebrand, H.F., Leroy, J.M., The relative influence of the topography and chemistry of TiA16V4 surfaces on osteoblastic cell behaviour (2000) Biomaterials, 21 (15), pp. 1567-1577; 
Deligianni, D.D., Katsala, N.D., Koutsoukos, P.G., Missirlis, Y.F., Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength (2001) Biomaterials, 22 (1), pp. 87-96; 
Boyan, B.D., Lossdorfer, S., Wang, L., Zhao, G., Lohmann, C.H., Cochran, D.L., Schwartz, Z., Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies (2003) Eur Cell Mater, 6, pp. 22-27; 
Brunette, D.M., The effects of implant surface topography on the behavior of cells (1988) Int J Oral Maxillofac Implants, 3 (4), pp. 231-246; 
Schwartz, Z., Lohmann, C.H., Oefinger, J., Bonewald, L.F., Dean, D.D., Boyan, B.D., Implant surface characteristics modulate differentiation behavior of cells in the osteoblastic lineage (1999) Adv Dent Res, 13, pp. 38-48; 
Shu, R., McMullen, R., Baumann, M.J., McCabe, L.R., Hydroxyapatite accelerates differentiation and suppresses growth of MC3T3-E1 osteoblasts (2003) J Biomed Mater Res, 67 A (4), pp. 1196-1204; 
Nordstrom, E., Ohgushi, H., Yoshikawa, T., Yokobori, A.T., Yokobori, T., Osteogenic differentiation of cultured marrow stromal stem cells on surface of microporous hydroxyapatite based mica composite and macroporous synthetic hydroxyapatite (1999) Biomed Mater Eng, 9 (1), pp. 21-26; 
Oreffo, R.O.C., Driessens, F.C.M., Flanell, J.A., Triffitt, J.T., Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements (1998) Biomaterials, 19 (20), pp. 1845-1854; 
Knabe, C., Berger, G., Gildenhaar, R., Klar, F., Zreiqat, H., The modulation of osteogenesis in vitro by calcium titanium phosphate coatings (2004) Biomaterials, 25, pp. 4911-4919
},
	month = {2005///},
	pages = {57 - 66},
	abstract = {In this study, the behavior of bone marrow stromal cells cultured on calcium titanium phosphate (CTP) microspheres was analyzed. Cell adhesion and proliferation were estimated by the neutral red assay and by total DNA quantification. Morphology and deposition of extracellular matrix were assessed by confocal laser scanning microscopy and/or scanning electron microscopy. The expression of the osteoblastic phenotype was evaluated by monitoring alkaline phosphatase activity and osteocalcin secretion. Results revealed that cells were able to attach and spread on the surface of CTP microspheres, and gradually grow into nearly confluent monolayers. Moreover, cells were able to bridge adjacent microspheres forming microsphere-cell clusters. Cells produced an abundant amount of fibrillar extracellular matrix that covered the substrate surface. Alkaline phosphatase activity peaked around days 7-14 and then decreased until day 21. Cells secreted osteocalcin, with higher levels being detected at day 14 than at day 21. Taken together, these results suggest that CTP microspheres are appropriate scaffolds for the growth and differentiation of cells along the osteoblastic lineage. {\textcopyright} 2004 Wiley Periodicals, Inc.},
	keywords = {alkaline phosphatase, animal cell, Animals, article, Bioassay, Bone, bone marrow cell, Bone Marrow Cells, bone marrow stromal cell, Bone marrow stromal cells, Calcium, Calcium Compounds, calcium phosphate, Calcium titanium phosphate, cell adhesion, Cell culture, cell differentiation, cell growth, cell lineage, cell proliferation, cell structure, Confluent monolayers, confocal laser microscopy, Confocal laser scanning microscopy, controlled study, Deposition, DNA determination, enzyme activity, Enzyme kinetics, extracellular matrix, Microscopy, Electron, microsphere, Microspheres, nonhuman, osteoblast, osteocalcin, Osteogenesis, Osteogenic differentiation, phenotype, protein expression, protein secretion, rat, Rats, Scanning electron microscopy, Stromal Cells, surface property, Tissue culture, Tissue engineering, Titanium, unclassified drug},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-10444223137\&partnerID=40\&md5=ab1b84ba6dd86274c0102567dffe1418},
	author = {Barrias, C.C. and Ribeiro, C.C. and Lamghari, M. and S{\'a} Miranda, C. and Barbosa, M.A.}
}
