



@article {2473,
	title = {Protein electrostatic self-assembly on poly(DL-lactide) scaffold to promote osteoblast growth},
	journal = {Journal of Biomedical Materials Research - Part B Applied BiomaterialsJ. Biomed. Mater. Res. Part B Appl. Biomater.},
	volume = {71},
	year = {2004},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: ScopusCODEN: JBMRGdoi: 10.1002/jbm.b.30080PubMed ID: 15368240Language of Original Document: EnglishCorrespondence Address: Ji, J.; Dept. of Poly. Sci. and Engineering, Zhejiang University, Hangzhou 310027, China; email: jianji@mail.hz.zj.cnChemicals/CAS: DNA, 9007-49-2; gelatin, 9000-70-8; polyethyleneimine, 74913-72-7; polylactide, 26680-10-4; DNA, 9007-49-2; Gelatin, 9000-70-8; poly(lactide), 26969-66-4; PolyestersReferences: Anselme, K., Osteoblast adhesion on biomaterials (2000) Biomaterials, 21, pp. 667-681;Kim, B.S., Mooney, D.J., Development of biocompatible synthetic extracellular matrices for tissue engineering (1998) Trend Biotechnol, 16, pp. 224-230; 
Hunter, A., Archer, C.W., Walker, P.S., Blunn, G.W., Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use (1995) Biomaterials, 16, pp. 287-295; 
Goldstein, A.S., Juarez, T.M., Helmke, C.D., Gustin, M.C., Mikos, A.G., Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds (2001) Biomaterials, 22, pp. 1279-1288; 
Liao, H., Andersson, A.S., Sutherland, D., Petronis, S., Kasemo, B., Thomsen, P., Response of rat osteoblast-like cells to microstructured model surfaces in vitro (2003) Biomaterials, 24, pp. 649-654; 
Bertrand, P., Jonas, A., Laschewsky, A., Legras, R., Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: Suitable materials, structure and properties (2000) Macromol Rapid Commun, 21, pp. 319-348; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Sano, M., Lvov, Y., Kunitake, T., Formation of ultrathin polymer layers on solid substrates by means of polymerization-induced epitaxy and alternate adsorption (1996) Ann Rev Mater Sci, 26, pp. 153-187; 
Laschewsky, A., Ultrathin polymer coatings by self-organization techniques. A critical comparison (1997) Eur Chem Chron, 2, pp. 13-24; 
Park, S.Y., Barrett, C.J., Rubner, M.F., Mayes, A.M., Anomalous adsorption of polyelectrolyte layers (2001) Macromolecules, 34, pp. 3384-3388; 
Sullivan, D.M., Bruening, M.L., Ultrathin, ion-selective polyimide membranes prepared from layered polyelectrolytes (2001) J Am Chem Soc, 123, pp. 11805-11806; 
Chluba, J., Voegel, J.C., Decher, G., Erbacher, P., Schaaf, P., Ogier, J., Peptide hormone covalently bound to polyelectrolytes and embedded into multilayer architectures conserving full biological activity (2001) Biomacromolecules, 2, pp. 800-805; 
Iler, R.K., Multilayers of colloidal particles (1966) J Colloid Interface Sci, 21, pp. 569-594; 
Decher, G., Hong, J.D., Buildup of ultrathin multilayer films by a self-assembly process: II. Consecutive adsorption of anionic and cationic bipolar amphiphiles and polyelectrolytes on charged surfaces (1991) Ber Bunsenges Phys Chem, 95, pp. 1430-1434; 
Decher, G., Fuzzy nanoassemblies: Toward layered polymeric multicomposites (1997) Science, 277, pp. 1232-1237; 
Lvov, Y., Decher, G., Moehwald, H., Assembly, structural characterization, and thermal behavior of layer-by-layer deposited ultrathin films of poly(vinyl sulfate) and poly(allylamine) (1993) Langmuir, 9, pp. 481-486; 
Lvov, Y., Haas, H., Decher, G., Moehwald, H., Mikhailov, A., Mtchedlishvily, B., Morgunova, E., Vainshtein, B.S., Successive deposition of alternate layers of polyelectrolytes and a charged virus (1994) Langmuir, 10, pp. 4232-4236; 
Kleinfeld, E.R., Ferguson, G.S., Stepwise formation of multilayered nanostructural films from macromolecular precursors (1994) Science, 265, pp. 370-373; 
Hong, D., Lowack, K., Schmitt, J., Decher, G., Layer-by-layer deposited multilayer assemblies of polyelectrolytes and proteins: From ultrathin films to protein arrays (1993) Progr Colloid Polym Sci, 93, pp. 98-102; 
Schmitt, J., Grunewald, T., Decher, G., Pershan, P.S., Krajer, K., Loesche, M., Internal structure of layer-by-layer adsorbed poly-electrolyte films: A neutron and X-ray reflectivity study (1993) Macromolecules, 26, pp. 7058-7063; 
Korneev, D., Lvov, Y., Decher, G., Schmitt, J., Yaradaikin, S., Neutron reflectivity analysis of self-assembled film super lattices with alternate layers of deuterated and hydrogenated polystyrenesulfonate and polyallylamine (1995) Physica B, 213-214, pp. 954-956; 
Zhu, H., Ji, J., Tan, Q., Barbosa, M.A., Shen, J., Surface engineering of poly (DL-lactide) via electrostatic self-assembly of extracellular matrix-like molecules (2003) Biomacromolecules, 4, pp. 378-386; 
Zhu, Y., Gao, C., He, T., Liu, X., Shen, J., Layer-by-layer assembly to modify poly(1-lactic acid) surface toward improving its cytocompatibility to human endothelial cells (2003) Biomacromolecules, 4, pp. 446-452; 
Miko, A.G., Sarakinos, G., Vacanti, J.P., Langer, R.S., Cima, L.G., Biocompatible polymer membranes and methods of preparation of three dimensional membrane structures. U. S. Patent No. 5,514378; 1996Instructions: BCA Protein Assay Reagent Kit 23227, , Pierce; 
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., Measurement of protein using bicinchoninic acid (1985) Anal Biochem, 150, pp. 76-85; 
(1998) Product Information: DNA Quantitation Kit Fluorescence Assay (DNA-QF), , SIGMA; 
West, D.C., Sattar, A., Kumar, S., A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells (1985) Anal Biochem, 147 (2), pp. 289-295; 
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J Immunol Methods, 65, pp. 55-63; 
Zund, G., Ye, Q., Hoerstrup, S.P., Schoeberlein, A., Schmid, A.C., Grunenfelder, J., Vogt, P., Turina, M., Tissue engineering in cardiovascular surgery: MTT, a rapid and reliable quantitative method to assess the optimal human cell seeding on polymeric meshes (1999) Eur J Cardio-thoracic Surg, 15, pp. 519-524
},
	month = {2004///},
	pages = {159 - 165},
	abstract = {The development of protein coating on 3D biodegradable scaffold based on electrostatic self-assembly (ESA) to promote osteoblast growth is reported. Poly (ethylenimine) (PEI) was employed to obtain a stable positively charged surface on poly(DL-lactide) (PDL-LA) substrate. An extracellular-matrix (ECM)-like biomacromolecule, gelatin, was chosen as the polyelectrolyte to deposit on the activated PDL-LA substrate via ESA technique. Osteoblast (MC3T3) was then cultured on unmodified and gelatin-modified PDL-LA scaffolds. Osteoblast testing regarding total intracellular protein content, total DNA content, cell activity, and cell morphology on the ECM-like multilayer-modified PDL-LA scaffold showed that osteoblast growth was promoted. It will be easy to replace the gelatin with osteoinductive proteins or other polyelectrolytes to promote specific osteoblast functions. In comparison with conventional coating methods, polyelectrolyte multilayers are easy and stable to prepare. They may be a good choice for the surface modification of complex biomedical devices, especially for the 3D tissue-engineering scaffold. These very flexible systems allow broad medical applications for drug delivery and tissue engineering. {\textcopyright} 2004 Wiley Periodicals, Inc.},
	keywords = {3T3 Cells, Absorbable Implants, Activation analysis, animal cell, Animals, article, Biodegradation, Biomedical engineering, cell activity, cell adhesion, Cell Division, cell growth, cell level, cell protein, cell structure, cell surface, Cell-material interactions, Cells, Coating techniques, DNA, DNA content, Drug products, Electrostatics, ethylene derivative, extracellular matrix, gelatin, Gelation, Growth (materials), Kinetics, macromolecule, materials testing, Mice, Microscopy, Confocal, mouse, nonhuman, osteoblast, Osteoblast growth, Osteoblast testing, Osteoblasts, polyelectrolyte, Polyelectrolytes, Polyesters, Polyethyleneimine, polylactide, polymer, protein assembly, Proteins, Surface modification, Tissue, Tissue engineering},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-4644230505\&partnerID=40\&md5=2c6499a5f15b19dfab9731021aa4639b},
	author = {Zhu, H. and Ji, J. and Barbosa, M.A. and Shen, J.}
}
