



@article {2474,
	title = {Calcium phosphate-alginate microspheres as enzyme delivery matrices},
	journal = {BiomaterialsBiomaterials},
	volume = {25},
	year = {2004},
	note = {Cited By (since 1996): 92Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2003.11.028PubMed ID: 15046927Language of Original Document: EnglishCorrespondence Address: Ribeiro, C.C.; Laborat{\'o}rio de Biomateriais, INEB, Inst. de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: cribeiro@ineb.up.ptChemicals/CAS: Alginates; alginic acid, 9005-32-7; calcium phosphate, 10103-46-5; Calcium Phosphates; Drug Carriers; Enzymes, Immobilized; Glucosylceramidase, EC 3.2.1.45; Glucuronic Acid, 576-37-4; Hexuronic Acids; PowdersReferences: Griffith, L.G., Naughton, G., Tissue engineering: Current challenges and expanding opportunities (2002) Science, 295, pp. 1009-1014;Hench, L.L., Polak, J.M., Third-generation biomedical materials (2002) Science, 295, pp. 1014-1017; 
Temenoff, J.S., Mikos, A.G., Injectable biodegradable materials for orthopedic tissue engineering (2000) Biomaterials, 21, pp. 2405-2412; 
Laurencin, C.T., Lu, H.H., Polymer-ceramic composites for bone tissue engineering (2000) Bone Engineering, pp. 463-472. , J.E. Davies. Toronto, Canada: EM Squared Inc; 
Gauthier, O., Bouler -., J.M., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Dupraz, A., Delecrin, J., Moreau, A., Pilet, P., Passut, N., Long term bone response to particulate injectable ceramic (1998) J Biomed Mater Res, 42, pp. 368-375; 
Grimandi, G., Weiss, P., Millot, F., Daculsi, G., In vitro evaluation of a new injectable calcium phosphate material (1998) J Biomed Mater Res, 39, pp. 660-666; 
Gauthier, O., Boix, D., Grimandi, G., Aguado, E., Bouler, J.M., Weiss, P., Daculsi, G., A new injectable phosphate biomaterial for immediate bone filling of extraction sockets: A preliminary study in dogs (1999) J Periodontol, 70, pp. 375-383; 
Peter, S.J., Nolley, J.A., Widmer, M.S., Mervin, J.E., Yaszemski, M.J., Yasko, A.W., Engel, P.S., Mikos, A.G., In vitro degradation of a poly (propylene fumarate)/β-tricalcium phosphate composite orthopedic scaffold (1997) Tissue Eng, 3, pp. 207-215; 
Sims, C.D., Butler, P.E.M., Casanova, R., Lee, B.T., Randolph, M.A., Lee, W.P.A., Vacant, C.A., Yamremchuk, M.J., Injectable cartilage using polyethylene oxide polymer substrates (1996) Plast Reconstr Surg, 98, pp. 843-850; 
Suggs, L.J., Shive, M.S., Garcia, C.A., Anderson, J.M., Mikos, A.G., In vitro cytotoxicity and in vivo biocompalibility of poly (propylene fumarate-co-ethylene glycol) hydrogels (1999) J Biomed Mater Res, 46, pp. 22-32; 
Suggs, L.J., Krishnan, R.S., Garcia, C.A., Peter, S.J., Anderson, J.M., Mikos, A.G., In vitro and in vivo degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels (1998) J Biomed Mater Res, 42, pp. 312-320; 
Frazier, D.D., Lathi, V.K., Gerhart, T.N., Hayes, W.C., Ex vivo degradation of a poly (propylene glycol-fumarate) biodegradable particulate bone cement (1997) J Biomed Mater Res, 35, pp. 383-389; 
Kharas, G.B., Kamanetsky, M., Simantirakis, J., Beinlich, K.C., Rizzo, A.-.M.T., Caywood, G.A., Watson, K., Synthesis and characterization of fumarate-based polyesters for use in bioresorbable bone cement composites (1997) J Appl Polym Sci, 66, pp. 1123-1137; 
Paige, K.T., Cima, L.G., Yaremchuck, M.J., Vacant, J.P., Vacant, C.A., Injectable cartilage (1995) Plast Reconstr Surg, 96, pp. 1390-1400; 
Paige, K.T., Cima, L.G., Yaremchuck, M.J., Vacant, J.P., Vacant, C.A., De novo cartilage generation using calcium-alginate-chondrocyte constructs (1996) Plast Reconstr Surg, 97, pp. 168-180; 
Kulseng, B., Skjak-Braek, G., Ryan, L., Andersson, A., King, A., Faxvaag, A., Espevik, T., Transplantation of alginate microcapsules (1999) Transplantation, 67, pp. 978-984; 
Martinetti, R., Dolcini, L., Ravaglioli, A., Krajewski, A., Mangano, C., Experimental study on hydroxyapatite/N-carboxymethyl chitosan fillers (1997) Bioceramics, 10, pp. 503-506. , L. Sedel, \& C. Rey. Oxford, UK: Elsevier; 
Maruyama, M., Ito, M., In vitro properties of a chitosan-bonded self hardening paste with hydroxyapatite granules (1996) J Biomed Mater Res, 32, pp. 527-532; 
Dupraz, A., Nguyen, T.P., Richard, M., Daculsi, G., Passuti, N., Influence of a cellulosic ether carrier on the structure of biphasic calcium phosphate ceramic particles in an injectable composite material (1999) Biomaterials, 20, pp. 663-673; 
Ribeiro, C.C., Ma, B., Calcium-Titanium-Phosphate: A Potential Material to Be Used in the Biomedical Field, , submitted for publication; 
Alamo, J., Chemistry and properties of solids with NZP skeleton (1993) Solid State Ionics, 63, pp. 547-561; 
Takahiro, S., Motohiro, T., Hosono, H., Application of a microporous glass-ceramic with a skeleton of CaTi 4(PO 4) 6 to carriers for immobilization of enzymes (1991) J Ferment Bioeng, 72, pp. 384-391; 
Gross, U., Muller-Mai, C., Voigt, C., Mesgarian, M., Berger, G., Ploska, U., Tissue response in femur of rabbits after implantation of a new calcium titanium phosphate composition (2001) Key Eng Mater, 192-195, pp. 383-386; 
Otsuka, M., Matsuda, Y., Fox, J.L., Higuchi, W.I., Yu, D., Wong, J., A novel skeletal drug delivery system for anti bacterial drugs using self-setting hydroxyapatite cement (1990) Chem Pharm Bull (Tokyo), 38, pp. 3500-3502; 
Guicheux, J., Grimandi, G., Trecant, M., Faivre, A., Takahashi, S., Apatite as a carrier for growth hormone: In vitro characterization of loading and release (1997) J Biomed Mater Res, 34, pp. 165-170; 
Gombotz, W.R., Wee, S.F., Protein release from alginate matrices (1998) Adv Drug Deliv Rev, 31, pp. 267-285; 
Eiselt, P., Yeh, J., Latvala, R.K., Shea, L.D., Mooney, D.J., Porous carriers for biomedical applications based on alginate hydrogels (2000) Biomaterials, 21, pp. 1921-1927; 
Alsberg, V., Anderson, K., Albeiruti, A., Franceshi, R.T., Mooney, D.J., Cell interactive alginate hydrogels for bone tissue engineering (2001) J Dent Res, 80, pp. 2025-2029; 
Smidsrod, O., Draget, K.I., Chemistry and physical properties of alginates (1996) Carbohyd Europe, 14, pp. 6-13; 
Sivakumar, M., Manjubala, I., Panduranga Rao, K., Preparation, characterization and in-vitro release of gentamicin from coralline hydroxyapatite-chitosan composite microspheres (2002) Carbohyd Pol, 49, pp. 281-288; 
Qiu, Q.Q., Ducheyne, P., Ayyaswamy, P.S., New bioactive, degradable composite microspheres as tissue engineering substrates (2000) J Biomed Mater Res, 52, pp. 66-76; 
Hsu, F.Y., Chueh -., S.C., Wang, Y.J., Microspheres of hydroxyapatite/reconstituted collagen as supports for osteoblast cell growth (1999) Biomaterials, 20, pp. 1931-1936; 
Grabowski, G.A., Leslie, N., Wenstrup, R., Enzyme therapy for Gaucher disease: The first 5 years (1998) Blood Rev, 12, pp. 115-133; 
Xu, Y.H., Ponce, E., Sun, Y., Leonova, T., Bove, K., Witte, D., Grabowski, G.A., Turnover and distribution of intravenously administered mannose-terminated human acid beta glucosidase in murine and human tissues (1996) Pediatr Res, 39, pp. 313-322; 
Ferraz, M.P., Monteiro, F.J., Serro, A.P., Saramago, B., Gibson, I.R., Santos, J.D., Effect of chemical composition on hydrophobicity and zeta-potential of plasma spayed HA/CaO-P 2O 5 glass coatings (2001) Biomaterials, 22, pp. 3105-3112; 
Lopes, M.A., Monteiro, F.L., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tension, and zeta potential measurements of glass reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Dupuy, B., Arien, A., Minnot, A.P., FT-IR membranes made with alginate/polylysine complexes. Variations with mannuronic or guluronic content of the polysaccharides. Art Cells, Blood Subs (1994) Immob Biotech, 20, pp. 71-82; 
Peters, T., (1996) All about Albumin: Biochemistry, Genetics and Medical Applications, , San Diego, CA: Academic Press Inc; 
Nakanishi, K., Sakiyama, T., Imamura, K., On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon (2001) J Biosci Bioeng, 91, pp. 233-244
},
	month = {2004///},
	pages = {4363 - 4373},
	abstract = {The present study concerns the preparation and initial characterisation of novel calcium titanium phosphate-alginate (CTP-alginate) and hydroxyapatite-alginate (HAp-alginate) microspheres, which are intended to be used as enzyme delivery matrices and bone regeneration templates. Microspheres were prepared using different concentrations of polymer solution (1\% and 3\%w/v) and different ceramic-to-polymer solution ratios (0.1, 0.2 and 0.4w/w). Ceramic powders were characterised using X-ray diffraction, laser granulometry, Brunauer, Emmel and Teller (BET) method for the determination of surface area, zeta potential and Fourier transform infrared spectroscopy (FT-IR). Alginate was characterised using high performance size exclusion chromatography. The methodology followed in this investigation enabled the preparation of homogeneous microspheres with a uniform size. Studies on the immobilisation and release of the therapeutic enzyme glucocerebrosidase, employed in the treatment of Gaucher disease, were also performed. The enzyme was incorporated into the ceramic-alginate matrix before gel formation in two different ways: pre-adsorbed onto the ceramic particles or dispersed in the polymeric matrix. The two strategies resulted in distinct release profiles. Slow release was obtained after adsorption of the enzyme to the ceramic powders, prior to preparation of the microspheres. An initial fast release was achieved when the enzyme and the ceramic particles were dispersed in the alginate solution before producing the microspheres. The latter profile is very similar to that of alginate microspheres. The different patterns of enzyme release increase the range of possible applications of the system investigated in this work. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {Absorption, Adsorption, Alginate, Alginates, article, Biomaterials, Bone, Calcium Compounds, Calcium phosphates, Calcium titanium phosphate, calcium titanium phosphate alginate, ceramics, Drug Carriers, Drug Delivery Systems, Enzyme delivery, Enzyme-delivery, Enzymes, Enzymes, Immobilized, Fourier transform infrared spectroscopy, Glucosylceramidase, Glucuronic Acid, Hexuronic Acids, High performance liquid chromatography, hydroxyapatite, hydroxyapatite alginate, laser, methodology, microsphere, Microspheres, Molecular Conformation, Particle Size, Powders, priority journal, surface property, unclassified drug, X ray diffraction},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1642331315\&partnerID=40\&md5=217590793385cbaff039a6b260d3d85c},
	author = {Ribeiro, C.C. and Barrias, C.C. and Barbosa, M.A.}
}
