



@article {2480,
	title = {Ultrastructural comparison of dissolution and apatite precipitation on hydroxyapatite and silicon-substituted hydroxyapatite in vitro and in vivo},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {69},
	year = {2004},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: JBMRCPubMed ID: 15162409Language of Original Document: EnglishCorrespondence Address: Porter, A.E.; Dept. of Mat. Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge, CB2 3QZ, United Kingdom; email: aep30@cam.ac.ukChemicals/CAS: apatite, 64476-38-6; carbonic acid, 3812-32-6, 463-79-6; hydroxyapatite, 1306-06-5, 51198-94-8; silicon, 7440-21-3; Biocompatible Materials; Durapatite, 1306-06-5; Silicon, 7440-21-3References: Porter, A.E., Best, S.M., Bonfield, W., Ultrastructural comparison of hydroxyapatite and silicon-substituted hydroxyapatite for biomedical applications (2003) J Biomed Mater Res, 68 A, pp. 133-141;Patel, N., Gibson, I.R., Hing, K.A., Best, S.M., Revell, P.A., Bonfield, W., A comparative study on the in vivo behaviour of hydroxyapatite and silicon substituted hydroxyapatite granules (2002) J Mater Sci Mater Med, 13, pp. 1199-1206; 
Porter, A.E., Patel, N., Skepper, J.N.S., Best, S.M., Bonfield, W., Comparison of in vivo dissolution processes in hydroxyapatite and silicon-hydroxyapatite bioceramics (2003) Biomaterials, 24, pp. 4609-4620; 
Weng, J., Liu, Q., Wolke, J.G.C., Zhang, X., De Groot, K., Formation and characteristics of the apatite layer on plasma-sprayed hydroxyapatite coatings in simulated body fluid (1995) Biomaterials, 18, pp. 1027-1035; 
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},
	month = {2004///},
	pages = {670 - 679},
	abstract = {Recent histological studies have demonstrated that the substitution of silicate ions into hydroxyapatite (HA) significantly increases the rate of bone apposition to HA implants. The enhanced bioactivity of silicon-substituted HA (Si-HA) over pure HA has been attributed to the effect of silicate ions in accelerating dissolution. In the present study, high-resolution transmission electron microscopy (HR-TEM) was employed to compare dissolution of HA and Si-HA in an acellular simulated body fluid (SBF) to dissolution in an in vivo model. HR-TEM observations confirmed a difference in morphology of apatite precipitates in vivo and in SBF: apatite deposits were platelike in vivo and nodular in SBF. Compositional mapping suggested that preferential dissolution of silicon from the implant promotes the nucleation of carbonate apatite around the implant. The in vivo findings illustrated an absence of dissolution at the bone-HA or Si-HA interface, whereas dissolution was extensive from within the implant. The amount of dissolution in acellular SBF was similar to dissolution from within the implant, although the site at which the dissolution nucleates was different: dissolution predominates at the crystallite surfaces in SBF, whereas grain boundary dissolution predominates in vivo. These findings suggest that proteins in the in vivo milieu modify the processes of dissolution from the implant. {\textcopyright} 2004 Wiley Periodicals, Inc.},
	keywords = {Apatite, article, Bioactivity, Biocompatible Materials, body fluid, carbonic acid, comparative study, Composition, Dissolution, Durapatite, histology, hydroxyapatite, implant, Implants (surgical), in vitro study, in vivo study, Interfaces (materials), Microscopy, Electron, Microscopy, Electron, Scanning, morphology, precipitation, Precipitation (chemical), Silicon, Simulated body fluid, Simulated body fluids (SBF), simulation, transmission electron microscopy, ultrastructure},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2942558665\&partnerID=40\&md5=c262d4d218d8dba93d767c3b6f399564},
	author = {Porter, A.E. and Botelho, C.M. and Lopes, M.A. and Santos, J.D. and Best, S.M. and Bonfield, W.}
}
