



@book {2499,
	title = {In Vitro Mineralisation of Human Bone Marrow Cells Cultured on Bonelike{\textregistered}},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Costa, M.A.; Inst. Cie. Blomed. de Abel Salazar, Universidade do Porto, Largo Abel Salazar, 4000 Porto, PortugalReferences: Santos, J.D., Campbell, J.K., Winston, G.H., European Patent N{\textdegree} WO0068164, 2000Lopes, M.A., Knowles, J.C., Santos, J.D., (2000) Biomaterials, 21, p. 1905;Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res.:App. Biomater., 48, p. 734; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (2000) Biomaterials, 21, p. 1165; 
Ferraz, M.P., Fernandes, M., Santos, J.D., Monteiro, F.J., (2001) J. Mat. Sci.: Mat. Med., 12, p. 629; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mater. Res., 54, p. 463; 
Duarte, F., Santos, J.D., Afonso, A., Advanced Materials Forum, , in press; 
Coelho, M.J., Fernandes, M.H., (2000) Biomaterials, 21, p. 1095; 
Kleerekoper, M., (1996) Principles of Bone Biology, p. 1053. , Academic Press
},
	month = {2004///},
	pages = {821 - 824},
	address = {Porto},
	abstract = {Bonelike{\textregistered} is a CaO-P                        2O
                        5 based glass-reinforced hydroxyapatite (HA) designed to mimic the inorganic composition of the bone tissue. This work evaluates the response of human bone marrow cells to Bonelike{\textregistered} concerning cell proliferation and osteoblast differentiation. HA was used as control material. Results showed that Bonelike{\textregistered} allowed the proliferation of bone marrow cells and their complete differentiation, as evidenced by the formation of cell-mediated mineralisation. In comparison with HA, Bonelike{\textregistered} had a positive effect on the expression of alkaline phosphatase and also on the formation of a mineralised matrix, two osteoblast markers.
},
	keywords = {Bone, Bonelike{\textregistered}, Cell culture, cell proliferation, Composition, Grafts, Human bone marrow cells, hydroxyapatite, In vitro mineralisation, mineralization, Scanning electron microscopy, Sintering, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346255201\&partnerID=40\&md5=470ac94fd75d1a53b887d44f59fbf7d1},
	author = {Costa, M.A. and Gutierres, M. and Almeida, L. and Lopes, M.A. and Santos, J.D. and Fernandes, M.H.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
