



@article {2515,
	title = {Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly (hydroxyethylmethacrylate)) surfaces},
	journal = {Journal of Biomaterials Science, Polymer EditionJ. Biomater. Sci. Polym. Ed.},
	volume = {14},
	year = {2003},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: JBSEEdoi: 10.1163/156856203766652057PubMed ID: 12807146Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: cibacron blue f3ga, 12236-82-7, 53127-08-5; dextran, 87915-38-6, 9014-78-2; fibrinogen, 9001-32-5; polymacon, 25053-81-0, 25249-16-5, 98932-78-6; polyurethan, 61789-63-7; Albumins; Biocompatible Materials; Cibacron Blue F 3GA, 12236-82-7; Fibrinogen, 9001-32-5; Membranes, Artificial; Polyhydroxyethyl Methacrylate, 25249-16-5; TriazinesReferences: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials Science - an Introduction to Materials in Medicine, , (Eds), Academic Press, San Diego, CA;Greco, R.S., (1994) Implantation Biology, the Host Response and Biomedical Devices, , (Ed.), CRC Press, Ann Arbour, MI; 
Sapatnekar, S., Anderson, J.M., (1999) Handbook of Biomaterials Evaluation. Scientific, Technical and Clinical Testing of Implant Materials, p. 353. , A. F. von Recum (Ed.), Taylor \& Francis, Philadelphia, PA; 
Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., (1998) Biomaterials, 10, p. 147; 
Keogh, J.R., Eaton, J.W., (1994) J. Lab. Clin. Med., 121, p. 537; 
Sapatnekar, S., Kieswetter, K.M., Merritt, K., Anderson, J.M., Cahalan, L., Verhoeven, M., Hendriks, M., Cahalan, P., (1995) J. Biomed. Mater Res., 29, p. 247; 
Paulsson, M., Kober, M., Freij-Larsson Stollenwerk, C., Wessl{\'e}n, B., Ljungh, {\r A}., (1993) Biomaterials, 14, p. 845; 
Kopperschlager, G., Bohme, H.J., Hofmann, E., (1982) Adv. Biochem. Eng., 25, p. 101; 
Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannell, R., (1976) Biochem. J., 157, p. 301; 
Arica, M.Y., Testereci, H.N., Denizli, A., (1998) J. Chromatogr A., 799, p. 83; 
Denizli, A., Salih, B., Senel, S., Arica, M.Y., (1998) J. Appl. Polym. Sci., 68, p. 657; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., (2003) Biomaterials, 24. , (in press); 
Leatherbarrow, R.L., Dean, P.D.G., (1980) Biochem. J., 189, p. 27; 
Horbett, T.A., (1986) Techniques of Biocompatibility Tests, 2, p. 183. , D. F. Williams (Ed.), CRC Press, Boca Raton, FL; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., (2001) J. Biomed. Mater. Res., 55, p. 45; 
(1993) Iodine-125, A Guide to Radioiodination Techniques, 64. , Amersham Life Science; 
Schrader, B., (1995) Infrared and Raman Spectroscopy, , (Ed.), VCH, Weinheim; 
Lamba, N.M.K., Woodhouse, K.A., Cooper, S.L., (1998) Polyurethanes in Biomedical Applications, , (Eds), CRC Press, Boca Raton, FL; 
http://www.lasurface.com/Beamson, G., Briggs, D., (1992) High Resolution XPS of Organic Polymers - The Scienta ESCA300 Database, , (Eds), Wiley, Chichester; 
Morra, M., Cassinelli, C., (1995) J. Biomed. Mater. Res., 29, p. 39; 
Compagnini, A., Fisichella, S., Foti, S., Maccarrone, G., Saletti, R., (1996) J. Chromatogr. A, 736, p. 115; 
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Kasori, Y., Shiroya, T., Shimura, K., Mochizuki, A., (2000) Biomaterials, 21, p. 1471; 
Feng, L., Andrade, J.D., (1994) Biomaterials, 15, p. 323; 
Peters T., Jr., (1996) All About Albumin. Biochemistry, Genetics and Medical Applications, , (Ed.), Academic Press, San Diego, CA; 
Dockal, M., Carter, D.C., R{\"u}ker, F., (1999) J. Biol. Chem., 274, p. 29303; 
Denizli, A., Kocakulak, M., Piskin, E., (1998) J. Chromatogr. B., 707, p. 25; 
Salih, B., Zenobi, R., (1998) Anal. Chem., 70, p. 1536; 
{\"O}sterberg, E., Bergstr{\"o}m, K., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., (1995) J. Biomed. Mater. Res., 29, p. 741; 
Beeskow, T., Kroner, K.H., Anspach, F.B., (1997) J. Colloid. Interf. Sci., 196, p. 278; 
Frazier, R.A., Matthijs, G., Davies, M.C., Roberts, C.J., Schacht, E., Tendler, S.J.B., (2000) Biomaterials, 21, p. 957; 
Bertilsson, L., Butt, H.J., Nelles, G., Schlereth, D.D., (1997) Biosens. Bioelectron., 12, p. 839; 
Kao, J.M., Rose, R., Yousef, M., Hunter, S.K., Rodgers, V.G.J., (1999) J. Biomed. Mater. Res., 47, p. 537; 
Tegoulia, V.A., Cooper, S.L., (2002) Colloid. Surf. B: Biointerf., 24, p. 217
},
	month = {2003///},
	pages = {439 - 455},
	abstract = {In the present work, it is intended to study the effect of Cibacron blue F3G-A (CB) immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces on protein adsorption and bacterial adhesion. CB immobilisation was carried out by covalent binding between its triazine ring and the hydroxyl groups of the polymer. Characterisation of the films was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR), contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). CB efficiency was evaluated using radiolabelled albumin and fibrinogen from pure solutions, mixtures of both and plasma. Bacterial adhesion tests before and after albumin pre-coating were also performed. The presence of CB increases albumin and fibrinogen adsorption to PU-PHEMA surfaces. The incorporation of CB onto the PU-PHEMA surface also increases bacterial adhesion. Although albumin pre-coating decreases bacterial adhesion onto PU (67\% decrease) and PU-PHEMA-CB (80\%), bacterial adhesion is always lower on PU and PU-PHEMA surfaces than on PU-PHEMA-CB. These results demonstrate that, in contrast to what has been described for CB bound to dextran, CB immobilisation on PU-PHEMA surfaces presents low selectivity to albumin and increased bacterial adhesion relatively to PU and PU-PHEMA surfaces.},
	keywords = {Adsorption, Albumin, Albumins, article, Bacterial adhesion, bacterium adherence, Biocompatible Materials, Chemical structure, Cibacron blue, cibacron blue f3ga, Contact angle, controlled study, covalent bond, dextran, fibrinogen, hydroxyl group, immobilization, infrared spectroscopy, isotope labeling, Membranes, Artificial, Microscopy, Electron, Scanning, nonhuman, PHEMA, Polyhydroxyethyl Methacrylate, polymacon, polyurethan, priority journal, Protein adsorption, Scanning electron microscopy, Spectroscopy, Fourier Transform Infrared, Staphylococcus epidermidis, Surface Properties, triazine derivative, Triazines, X ray photoelectron spectroscopy, XPS},
	isbn = {09205063 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038316702\&partnerID=40\&md5=6f911799b67ba3289c643eb874879e1f},
	author = {Martins, M.C.L. and Wang, D. and Ji, J. and Feng, L. and Barbosa, M.A.}
}
