



@article {2517,
	title = {Albumin and fibrinogen adsorption on PU-PHEMA surfaces},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 41Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(03)00002-4PubMed ID: 12628827Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; Laborat{\'o}rio de Biomateriais, Inst. de Engenharia Biom{\'e}dica, Rua do Campo Algre 823, Porto 4150-180, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: acrylic acid ethyl ester, 140-88-5; fibrinogen, 9001-32-5; poly(2 hydroxyethyl acrylate), 26022-14-0; polyetherurethan, 76600-67-4; polymacon, 25053-81-0, 25249-16-5, 98932-78-6; protein, 67254-75-5; Albumins; Biocompatible Materials; Fibrinogen, 9001-32-5; poly(2-hydroxyethyl acrylate), 26022-14-0; Polyhydroxyethyl Methacrylate, 25249-16-5; Water, 7732-18-5References: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials science - An introduction to materials in medicine, , CA, USA, San Diego: Academic Press;Greco, R.S., (1994) Implantation biology, the host response and biomedical devices, , Ann Arbour, USA: CRC Press; 
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},
	month = {2003///},
	pages = {2067 - 2076},
	abstract = {Materials that adsorb specific proteins may find a variety of applications in the biomedical field. The aim of this study was the preparation of a hydrophilic surface, with low protein adsorption, to be used in the future as a support for the immobilisation of several species, e.g. Cibacron Blue F3G-A, which has been described to induce specific albumin adsorption. Poly(hydroxyethylmethacrylate) (PHEMA) and poly(hydroxyethylacrylate) (PHEA) were chosen as the hydrophilic surface because they can be easily polymerised and possess hydroxyl groups that can be used for the immobilisation of different compounds. Thin films of PHEMA and PHEA were successfully graft polymerised onto the surface of a commercial poly(etherurethane) (PU) using ceric ion as initiator. Grafting polymerisations were followed by mass gain and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Since stability tests demonstrated that only PU-PHEMA was stable in alkaline solutions, a necessary condition to future immobilisations, the investigation was focused on the coating of PU with PHEMA. PU-PHEMA films were characterised in detail using several techniques as mass gain, ATR-FTIR, contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Protein adsorption was evaluated using radiolabelled albumin and fibrinogen from pure solutions and from mixtures of both proteins. PU surfaces modified with PHEMA have demonstrated low protein adsorption, showing their potential use as substrates. This opens the possibly of exploring the advantages of selective adsorption by appropriate immobilisation of specific molecules. {\textcopyright} 2003 Elsevier Science Ltd. All rights reserved.},
	keywords = {acrylic acid ethyl ester, Adsorption, Albumin, Albumins, article, ATR-FTIR, Biocompatible Materials, Chemical modification, Dose-Response Relationship, Drug, fibrinogen, film, Fourier transform infrared spectroscopy, Graft polymerisation, Humans, Hydrogen-Ion Concentration, Hydrophilic surfaces, hydrophilicity, hydroxyl group, infrared spectroscopy, isotope labeling, Microscopy, Electron, Scanning, molecular stability, Pellethane, PHEMA, poly(2 hydroxyethyl acrylate), polyetherurethan, Polyhydroxyethyl Methacrylate, polymacon, Polymerization, priority journal, protein, Protein adsorption, Proteins, Scanning electron microscopy, Spectrometry, X-Ray Emission, Spectroscopy, Fourier Transform Infrared, surface property, Thin films, unclassified drug, Water, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037400503\&partnerID=40\&md5=dc989d7fe65e35d20f250cf3e7c245f9},
	author = {Martins, M.C.L. and Wang, D. and Ji, J. and Feng, L. and Barbosa, M.A.}
}
