



@article {2520,
	title = {Surface engineering of poly(DL-lactide) via electrostatic self-assembly of extracellular matrix-like molecules},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {4},
	year = {2003},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm025773pPubMed ID: 12625735Language of Original Document: EnglishCorrespondence Address: Ji, J.; Department of Polymer Science/Eng., Zhejing University, Hangzhou 310027, China; email: jianji@mail.hz.zj.cnChemicals/CAS: gelatin, 9000-70-8; polyethyleneimine, 74913-72-7; polylactide, 26680-10-4; poly(lactide), 26969-66-4; PolyestersTradenames: AXIOVERT 200, Zeiss; ESCA LAB 200A, VG; Radiance 2100, Biorad; Shimadzu UV-1201, Shimadzu; SPA 400, Seiko; Vectro 22, BrukerManufacturers: Biorad; Bruker; Seiko; Shimadzu; VG; ZeissReferences: Sittinger, M., Perka, C., Schutz, O., Haupl, T., Burmester, G.R.Z., (1999) Rheumatol., 58, pp. 130-135;Kim, B.S., Mooney, D.J., (1998) Trends Biotechnol., 16, p. 224; 
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},
	month = {2003///},
	pages = {378 - 386},
	abstract = {We report the development of new biomacromolecule coatings on biodegradable biomaterials based on electrostatic assembly of extracellular matrix-like molecules. Poly(ethylene imine) (PEI) was employed to engineer poly(DL-lactide) (PDL-LA) substrate to obtain a stable positively charged surface. An extracellular matrix- (ECM-) like biomacromolecule, gelatin, was selected as the polyelectrolyte to deposit on the activated PDL-LA substrate via the electrostatic assemble technique. The extracellular matrix-like multilayer on the PDL-LA substrate was investigated by attenuated total reflection (ATR-FTIR), X-ray photoelectron spectrscopy (XPS), contact angle, and atomic force microscopy (AFM). The gradual buildup of the protein layer was investigated by UV-vis spectra, and it was further given a quantitative analysis of the protein layer on the PDL-LA substrate via the radioiodination technique. The stability of the protein layer under aqueous condition was also tested by the radiolabeling method. Chondrocyte was selected as the model system for testing the cell behavior and morphology on modified PDL-LA substrates. The chondrocyte test about cell attachment, proliferation, cell activity and cell morphology by SEM, and confocal laser scanning microscopy (CLSM) investigation on extracellular matrix-like multilayer modified PDL-LA substrate was shown to promote chondrocyte attachment and growth. Comparing conventional coating methods, polyelectrolyte multiplayers are easy and stable to prepare. It may be a good choice for the modification of 3-D scaffolds used in tissue engineering. These very flexible systems allow broad medical applications for drug delivery and tissue engineering.},
	keywords = {animal cell, Animalia, Animals, article, Atomic force microscopy, biodegradability, biomaterial, cartilage cell, cell activity, cell adhesion, cell function, cell growth, cell proliferation, cell structure, Cells, Cultured, Chondrocytes, confocal laser microscopy, controlled study, Electron Probe Microanalysis, Electrostatics, extracellular matrix, Fourier transform infrared spectroscopy, gelatin, macromolecule, Macromolecules, Microscopy, Atomic Force, nonhuman, polyelectrolyte, Polyesters, Polyethyleneimine, Polyethylenes, polylactide, priority journal, protein analysis, protein stability, quantitative analysis, Rabbits, radioiodination, Scanning electron microscopy, Self assembly, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Surface charge, Surface engineering, Surface Properties, Tissue engineering, Ultraviolet spectroscopy, X ray photoelectron spectroscopy},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038071804\&partnerID=40\&md5=dc56622e3535f161005efe1ae86f4d23},
	author = {Zhu, H. and Ji, J. and Tan, Q. and Barbosa, M.A. and Shen, J.}
}
