



@article {2524,
	title = {Si                    3N
                    4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells
},
	journal = {BiomaterialsBiomaterials},
	volume = {23},
	year = {2002},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(02)00249-1PubMed ID: 12361631Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Universidade do Porto, Rua Dr Manuel Pereira da Silva, 4200-393 Porto, Portugal; email: mhrf@portugalmail.ptChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; Calcium, 7440-70-2; Phosphorus, 7723-14-0; Silicon Compounds; silicon nitride, 12033-89-5References: Amaral, M., Lopes, M.A., Silva, R.F., Santos, J.D., Densification route and mechanical properties of Si 3N 4-bioglass biocomposites (2002) Biomaterials, 23, pp. 857-862;Kue, R., Sahrabi, A., Nagle, D., Frondonza, C., Hungerford, D., Enhanced proliferation and osteocalcin production by human osteoblast-like cells on silicon nitride ceramic discs (1999) Biomaterials, 20, pp. 1195-1201; 
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},
	month = {2002///},
	pages = {4897 - 4906},
	abstract = {The in vitro osteocompatibility of a novel Si                        3N
                        4-bioglass composite (70-30\% weight proportion) with improved mechanical properties (fracture toughness=4.4MPam
                        1/2; bending strength=383{\textpm}47MPa) is reported. Immersion of the composite samples in culture medium (30min to 7 days) resulted in rapid protein adsorption to the surface and, also, dissolution of the intergranular phase of bioglass (time-dependent process) with the formation of different size cavities. "As-received" and pre-treated material samples presented a similar behaviour concerning the proliferation of MG63 osteoblast-like cells, evaluated during a 5-day culture period. Seeded materials showed a higher cell growth rate as compared to cultures performed on the standard plastic culture plates. To assess the osteogenic potential of the composite, "as-received" material samples were seeded with human bone marrow cells and cultured for 35 days in experimental conditions that favour the development of the osteoblastic phenotype. The cell adhesion process was similar to that observed in control cultures. Cells successfully adapted to the irregularities of the surface and were able to grow towards inside the cavities; in addition, osteogenic differentiation occurred with the formation of abundant cell-mediated mineralised deposits. Results suggest that this Si
                        3N
                        4-bioglass composite seems to be a promising candidate for high-stress medical applications. {\textcopyright} 2002 Elsevier Science Ltd. All rights reserved.
},
	keywords = {3N, 4-bioglass composite, Adhesion, Adsorption, alkaline phosphatase, article, Biocompatibility, Bioglass, Biomaterials, bone marrow cell, Bone Marrow Cells, Calcium, cell adhesion, Cell Culture Techniques, cell differentiation, Cell Division, cell growth, cell proliferation, composite material, controlled study, culture medium, Dissolution, glass, Growth kinetics, human, Human bone marrow cells, human cell, Humans, immersion, in vitro study, Mechanical properties, MG63 osteoblast-like cells, Microscopy, Electron, Scanning, osteoblast, Osteoblasts, Osteocompatibility, Osteogenic differentiation, phenotype, phosphorus, priority journal, Proteins, rigidity, Si, Silicon compounds, silicon derivative, surface property, tensile strength, Time Factors},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036888731\&partnerID=40\&md5=2235d0ad5a92fb1ae088f736b6e7ca4d},
	author = {Amaral, M. and Costa, M.A. and Lopes, M.A. and Silva, R.F. and Santos, J.D. and Fernandes, M.H.}
}
