



@article {2580,
	title = {Flow cytometry for assessing biocompatibility},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {41},
	year = {1998},
	note = {Cited By (since 1996): 33Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19980915)41:4<649::AID-JBM17>3.0.CO;2-9PubMed ID: 9697038Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University of London, 256 Gray{\textquoteright}s Inn Rd., London WC1X 8LD, United KingdomChemicals/CAS: Biocompatible Materials; Collagen, 9007-34-5; Dental Implants; Fibronectins; Osteocalcin, 104982-03-8References: Kirkpatrick, C.J., A critical view of current and proposed methodologies for biocompatibility testing: Cytotoxicity in vitro (1992) Regul. Affairs, 4, pp. 13-32;Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Ariola, C.R., Granchi, D., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin. Mater., 15, pp. 173-190; 
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Macon, W.R., Salhany, K.E., TI- T-cell subset analysis of paraffin-embedded peripheral T-cell lymphomas (PTCLs) with correlation of CD4/CD8 results by flow cytometry (FACs) (1997) Lab. Invest., 76, p. 753; 
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Beauchamp, J.R., Abraham, D.J., Bou-Gharios, G., Partridge, T.A., Olsen, I., Expression and function of adhesion molecules during differentiation of human skeletal muscle in culture (1992) Am. J. Pathol., 140, pp. 387-401; 
Abraham, D., Lupoli, S., McWhirter, A., Plater-Zyberk, C., Piela, T.H., Korn, J., Olsen, I., Black, C., Expression and function of surface antigens on scleroderma fibroblasts (1991) Arthritis Rheum., 34, pp. 1164-1172; 
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Altankov, G., Grinnell, F., Groth, T., Studies on the biocompatibility of materials: Fibroblast reorganization of substratumbound fibronectin on surfaces varying in wettability (1996) J, Biomed. Mater. Res., 30, pp. 385-391; 
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Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J. Biomed. Mater. Res., 39, pp. 244-251
},
	month = {1998///},
	pages = {649 - 656},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Flow cytometry (FCM) was examined as a possible procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery. The human osteoblast-like cell line MG63 was grown on hydroxyapatite (HA) and P                        2O
                        5 glass-reinforced HA composite discs and compared with the same cells grown on polystyrene culture dishes. While morphological observation at the light and electron microscopic levels showed no major deleterious effects, FCM indicated that cell size was somewhat reduced, particularly by growth on the HA composite. Moreover, this material also appeared to delay the progression of the cells from the G0/G1 into the S phase of the cell cycle. In addition to this low level of inhibition of cell growth relative to control cultures, FCM analysis also demonstrated that the glass-reinforced HA caused some down-regulation of the expression of osteocalcin and fibronectin, two antigens which play a vital part in the integrity and function of bone and soft connective tissue, respectively. These results thus show, first, that although HA and the HA composite used in these experiments were generally biocompatible, they nevertheless had certain suboptimal effects on the cells; and second, that FCM could be a highly useful procedure for effectively screening and evaluating important biological responses to implant materials.
Flow cytometry (FCM) was examined as a possible procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery. The human osteoblast-like cell line MG63 was grown on hydroxyapatite (HA) and P
                        2O
                        5 glass-reinforced HA composite discs and compared with the same cells grown on polystyrene culture dishes. While morphological observation at the light and electron microscopic levels showed no major deleterious effects, FCM indicated that cell size was somewhat reduced, particularly by growth on the HA composite. Moreover, this material also appeared to delay the progression of the cells from the G0/G1 into the S phase of the cell cycle. In addition to this low level of inhibition of cell growth relative to control cultures, FCM analysis also demonstrated that the glass-reinforced HA caused some down-regulation of the expression of osteocalcin and fibronectin, two antigens which play a vital part in the integrity and function of bone and soft connective tissue, respectively. These results thus show, first, that although HA and the HA composite used in these experiments were generally biocompatible, they nevertheless had certain suboptimal effects on the cells; and second, that FCM could be a highly useful procedure for effectively screening and evaluating important biological responses to implant materials.
},
	keywords = {antigen expression, Antigens, Biocompatibility, Biocompatible Materials, biomaterial, Biomaterials, Biomedical engineering, Bone and Bones, bone prosthesis, Cell culture, cell cycle, cell cycle s phase, Cell Line, Cell size, cell ultrastructure, Cells, Collagen, conference paper, Dental Implants, dentistry, extracellular matrix, Fibronectin, Fibronectins, flow cytometry, Glass fiber reinforced plastics, growth inhibition, human, human cell, Humans, hydroxyapatite, Implants, Implants (surgical), Microscopy, Electron, Scanning, mitosis inhibition, morphology, orthopedics, Osteoblasts, osteocalcin, Polystyrenes, Prostheses and Implants, prosthesis material, Tissue, tooth prosthesis},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032531299\&partnerID=40\&md5=5ea21d124ffce48018967f988cac815c},
	author = {Lopes, M.A. and Knowles, J.C. and Kuru, L. and Santos, J.D. and Monteiro, F.J. and Olsen, I.}
}
