



@article {3388,
	title = {The interactions between municipal socioeconomic status and age on hip fracture risk},
	journal = {Osteoporosis International},
	volume = {26},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {489-498},
	isbn = {0937-941X},
	url = {<Go to ISI>://WOS:000349018700008},
	author = {Oliveira, C.M. and Economou, T. and Bailey, T. and Mendona, D. and Pina, M.F.}
}
@article {3311,
	title = {The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {8144 - 8153},
	abstract = {Intervertebral disc (IVD) degeneration is the leading cause of low back pain and disability in the active population. Transplantation of mesenchymal stem cells (MSCs) in a hydrogel carrier can induce regenerative effects in degenerated IVDs. Moreover, it was found that degenerative discs release chemoattractants effective in MSC recruitment. Based on these findings, we hypothesized that an injectable hydrogel that can enhance the number of migrated MSCs in the IVD and provide a suitable matrix for their survival and differentiation would be ideal. The purpose of this study was to evaluate the potential of a thermoreversible hyaluronan-poly(N-isopropylacrylamide) (HAP) hydrogel as chemoattractant delivery system to recruit human MSCs in degenerative IVDs. The results demonstrate that HAP hydrogels containing stromal cell derived factor-1 (SDF-1) significantly increased the number of MSCs migrating into nucleotomized discs compared with discs treated with only HAP or SDF-1 in solution. HAP hydrogels releasing SDF-1 enhanced both the number of recruited cells and their migration distance in the IVD tissue. Furthermore, this phenomenon was dependent on MSC donor age. In conclusion, HAP SDF-1 is effective for the recruitment of stem cells in the IVD, thus opening new possibilities for the development of regenerative therapies based on endogenous cell migration. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {Intervertebral disc, Mesenchymal Stem Cells, Recruitment, Stromal cell derived factor-1, Thermoreversible hyaluronan hydrogel},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903746142\&partnerID=40\&md5=1366ab2e3399d0a363aab2e356bbdf96},
	author = {Pereira, C.L. and Gon{\c c}alves, R.M. and Peroglio, M. and Pattappa, G. and D{\textquoteright}Este, M. and Eglin, D. and Barbosa, M.A. and Alini, M. and Grad, S.}
}
@article {3303,
	title = {Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure},
	journal = {ACS Nano},
	volume = {8},
	year = {2014},
	note = {Cited By (since 1996):4Export Date: 21 November 2014},
	month = {2014},
	pages = {2033 - 2047},
	abstract = {Stem cell responsiveness to extracellular matrix (ECM) composition and mechanical cues has been the subject of a number of investigations so far, yet the molecular mechanisms underlying stem cell mechano-biology still need full clarification. Here we demonstrate that the paralog proteins YAP and TAZ exert a crucial role in adult cardiac progenitor cell mechano-sensing and fate decision. Cardiac progenitors respond to dynamic modifications in substrate rigidity and nanopattern by promptly changing YAP/TAZ intracellular localization. We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design. {\textcopyright} 2014 American Chemical Society.},
	keywords = {adult cardiac progenitor cell, cardiac differentiation, mechano- transduction, substrate nanotopography, YAP/TAZ},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896911335\&partnerID=40\&md5=c15ea687a167227f6d91e71dcc812590},
	author = {Mosqueira, D. and Pagliari, S. and Uto, K and Ebara, M and Romanazzo, S. and Escobedo-Lucea, C and Nakanishi, J and Taniguchi, A and Franzese, O and Di Nardo, P. and Goumans, MJ and Traversa, E and Pinto-Do-{\'O}, P. and Aoyagi, T. and Forte, G.}
}
@article {3398,
	title = {Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model},
	journal = {Journal of Clinical Investigation},
	volume = {124},
	year = {2014},
	note = {Bestas, Burcu Moreno, Pedro M. D. Blomberg, K. Emelie M. Mohammad, Dara K. Saleh, Amer F. Sutlu, Tolga Nordin, Joel Z. Guterstam, Peter Gustafsson, Manuela O. Kharazi, Shabnam Piatosa, Barbara Roberts, Thomas C. Behlke, Mark A. Wood, Matthew J. A. Gait, Michael J. Lundin, Karin E. El Andaloussi, Samir Mansson, Robert Berglof, Anna Wengel, Jesper Smith, C. I. Edvard},
	month = {2014},
	pages = {4067-4081},
	isbn = {0021-9738},
	url = {<Go to ISI>://WOS:000341168100038},
	author = {Bestas, B. and Moreno, P.M.D. and Blomberg, K. E. M. and Mohammad, D. K. and Saleh, A. F. and Sutlu, T. and Nordin, J. Z. and Guterstam, P. and Gustafsson, M. O. and Kharazi, S. and Piatosa, B. and Roberts, T. C. and Behlke, M. A. and Wood, M. J. A. and Gait, M. J. and Lundin, K.E. and El Andaloussi, S. and Mansson, R. and Berglof, A. and Wengel, J. and Smith, C.I.E.}
}
@article {2834,
	title = {Development of PLGA-mannosamine nanoparticles as oral protein carriers},
	journal = {Biomacromolecules},
	volume = {14},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {4046 - 4052},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84887602933\&partnerID=40\&md5=ee1468ca288d3ca59120be3637a51b41},
	author = {Alonso-Sande, M. and Des Rieux, A. and Fievez, V. and Sarmento, B. and Delgado, A. and Evora, C. and Remu{\~n}{\'a}n-L{\'o}pez, C. and Pr{\'e}at, V. and Alonso, M.J.}
}
@article {2874,
	title = {Osteoporotic hip fractures: Bisphosphonates sales and observed turning point in trend. A population-based retrospective study},
	journal = {Bone},
	volume = {53},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {430 - 436},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873288549\&partnerID=40\&md5=da493b87546091513eb8b54c3748cda6},
	author = {Alves, S.M. and Economou, T. and Oliveira, C. and Ribeiro, A.I. and Neves, N. and Gom{\'e}z-Barrena, E. and Pina, M.F.}
}
@conference {2192,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical ImagingIEEE Comput. Soc. Conf. Comput. Vis. Pattern Recogn.},
	series = {2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012},
	year = {2012},
	note = {Conference code: 91746Export Date: 21 November 2012Source: ScopusArt. No.: 6235546doi: 10.1109/ISBI.2012.6235546Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Lutgens, E., Daemen, M., Muinck, E., Chronic myocardial infarction in the mouse: Cardiac structural and functional changes (1998) Cardiovascular Research, 41, pp. 586-593; 
Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Nascimento, D., Valente, M., Esteves, T., Pina, M.F., Guedes, J.G., Freire, A., Quelhas, P., Pinto-Do, O.P., Miquant - Semi-automation of infarct size assessment in models of cardiac ischemic injury (2011) Plos One, 6 (9), pp. 1-8; 
Esteves, T., Valente, M., Nascimento, D., Pinto-Do, O.P., Quelhas, P., Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model (2011) Proceedings of IbPRIA, LNCS, 6669, pp. 151-158; 
Pfeffer, M., Pfeffer, J., Fishbein, M., Myocardial infarct size and ventricular function in rats (1979) Circulation Research, 44, pp. 503-512
Sponsors: The Institute of Electrical and Electronic Engineers; Engineering in Medicine and Biology Society; Signal Processing Society},
	month = {2012///},
	pages = {310 - 313},
	address = {Barcelona},
	abstract = {Experimental rodent models of induced ischemic injury have been extensively used in biomedical research to study molecular, cellular and histological alterations following myocar-dial infarction. These models are increasingly employed to assess the potential of newly developed therapies for functional restoration of the damaged heart. Such studies are based on myocardial infarction induction followed by different therapeutic interventions and subsequent analysis of the infarct size. This analysis is used to evaluate the extent to which such interventions meet recovery of the lost myocardial tissue. Infarct size is defined as the percentage of the left ventricle affected by coronary artery occlusion. The infarct size is traditionally estimated manually delineating the infarcted and normal tissue areas in the left ventricle of the excised heart. However, this is a time-consuming, arduous and prone to bias process. Herein, we developed an anatomic model, adapted through expectation maximization, which allows for fully automatic analysis of the data. Experimental validation is performed comparing the proposed approach with manual annotation. The results obtained through anatomical model adaptation were coherent with those manually obtained and the differences where never higher than 10\%. {\textcopyright} 2012 IEEE.},
	keywords = {Anatomic models, anatomical model, Anatomical models, Automatic analysis, Biomedical research, Coronary arteries, expectation maximization, Experimental validations, Image segmentation, Infarct size, Ischemic injuries, Left ventricles, Manual annotation, Maximum principle, Medical imaging, Murine model, Myocardial Infarction, Myocardial infarction size, Myocardial tissue, Normal tissue, Rodent models, Therapeutic intervention, Tissue},
	isbn = {19457928 (ISSN); 9781457718588 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=087bcb09e5d5319029623283cba34c34},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@conference {2817,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical Imaging},
	year = {2012},
	note = {Export Date: 2 October 2013Source: ScopusArt. No.: 6235546},
	month = {2012///},
	pages = {310 - 313},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=6716e8eb5d812d3e0ffe54d87369d746},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@article {2206,
	title = {Gradient convergence filters and a phase congruency approach for in vivo cell nuclei detection},
	journal = {Machine Vision and ApplicationsMach Vision Appl},
	volume = {23},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: MVAPEdoi: 10.1007/s00138-012-0407-7Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB-Instituto de Engenharia Biom{\'e}dica, Oporto, Portugal; email: quelhas@fe.up.ptReferences: Bleau, A., Leon, L.J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370. , DOI 10.1006/cviu.1999.0822;Bresson, X., Vandergheynst, P., Thiran, J., A priori information in image segmentation: Energy functional based on shape statistical model and image information (2003) Proceedings of International Conference on Image Processing, pp. 428-428. , ICIP; 
Bunyak, F., Palaniappan, K., Nath, S.K., Baskin, T.I., Dong, G., Quantitative cellmotility for in vitrowound healing using level setbased active contour tracking (2011) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 1-4; 
Byun, J., Verardo, M.R., Sumengen, B., Lewis, G.P., Manjunath, B.S., Fisher, S.K., Automated tool for the detection of cell nuclei in digital microscopic images: Application to retinal images (2006) Mol. Vis., 12, pp. 949-960; 
Campilho, A., Garcia, B., Toorn, H.V.D., Wijk, H.V., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48 (4), pp. 619-627. , DOI 10.1111/j.1365-313X.2006.02892.x; 
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},
	month = {2012///},
	pages = {623 - 638},
	abstract = {Computational methods used in microscopy cell image analysis have largely augmented the impact of imaging techniques, becoming fundamental for biological research. The understanding of cell regulation processes is very important in biology, and in particular confocal fluorescence imaging plays a relevant role for the in vivo observation of cells. However, most biology researchers still analyze cells by visual inspection alone, which is time consuming and prone to induce subjective bias. This makes automatic cell image analysis essential for large scale, objective studies of cells. While the classic approach for automatic cell analysis is to use image segmentation, for in vivo confocal fluorescence microscopy images of plants, such approach is neither trivial nor is it robust to image quality variations. To analyze plant cells in in vivo confocal fluorescence microscopy images with robustness and increased performance, we propose the use of local convergence filters (LCF). These filters are based in gradient convergence and as such can handle illumination variations, noise and low contrast.We apply a range of existing convergence filters for cell nuclei analysis of the Arabidopsis thaliana plant root tip. To further increase contrast invariance, we present an augmentation to local convergence approaches based on image phase information. Through the use of convergence index filters we improved the results for cell nuclei detection and shape estimation when compared with baseline approaches. Using phase congruency information we were able to further increase performance by 11\% for nuclei detection accuracy and 4\% for shape adaptation. Shape regularization was also applied, but with no significant gain, which indicates shape estimation was good for the applied filters. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Biological research, Cell analysis, Cell images, cell nucleus, Cell regulation, Cell segmentation, Cells, Confocal fluorescence imaging, Confocal fluorescence microscopy, Convergence filters, cytology, Detection accuracy, Estimation, Fluorescence microscopy, Illumination variation, Image analysis, Image filtering, Image segmentation, Imaging techniques, In-vivo, Information use, Local Convergence, Local image filtering, Medical applications, Phase congruency, Phase information, Plant cells, Plant roots, Plants (botany), Quality variation, Shape adaptation, Shape estimation, Shape regularization, Visual inspection},
	isbn = {09328092 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865374061\&partnerID=40\&md5=8e2b458028b5492266f085546a6804d9},
	author = {Esteves, T. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2204,
	title = {Neuropeptide Y Y1 receptor antagonism increases bone mass in mice},
	journal = {BoneBone},
	volume = {51},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: BONEDdoi: 10.1016/j.bone.2012.03.020PubMed ID: 22484690Language of Original Document: EnglishCorrespondence Address: Herzog, H.; Neuroscience Research Program, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW 2010, Australia; email: h.herzog@garvan.org.auChemicals/CAS: corticosterone, 50-22-6; glucose, 50-99-7, 84778-64-3; n2 diphenylacetyl n (4 ureidomethylbenzyl)argininamide, 191868-14-1, 217977-06-5; neuropeptide Y, 82785-45-3, 83589-17-7; Arginine, 74-79-3; BIBO 3304; Glucose, 50-99-7; Receptors, Neuropeptide Y; neuropeptide Y-Y1 receptorTradenames: bibo 3304References: Karaguzel, G., Holick, M.F., Diagnosis and treatment of osteopenia (2010) Rev Endocr Metab Disord, 11, pp. 237-251;Burge, R., Dawson-Hughes, B., Solomon, D.H., Wong, J.B., King, A., Tosteson, A., Incidence and economic burden of osteoporosis-related fractures in the United States, 2005-2025 (2007) J Bone Miner Res, 22, pp. 465-475; 
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},
	month = {2012///},
	pages = {8 - 16},
	abstract = {The neuropeptide Y system has emerged as one of the major neural signalling pathways regulating bone homeostasis. Absence of Y1 receptor signalling from bone forming osteoblasts is responsible for an enhancement on bone mass in mice, suggesting that pharmacological blockade of Y1 receptors may offer a novel anabolic treatment option for improving bone mass. Here we show that oral administration of the selective Y1 receptor antagonist BIBO3304 for 8. weeks dose-dependently increases bone mass in mice. Histomorphometric analysis revealed a significant 1.5-fold increase in cancellous bone volume in the femora of mice treated with BIBO3304. Furthermore, bone microarchitecture was improved, with greater trabecular number and trabecular thickness. This increase in bone mass was associated with a significant increase in bone anabolic activity of osteoblasts and, interestingly, was evident despite a coincident increase in bone resorption, as evidenced by an increase in the number of the osteolytic osteoclasts. Changes were also evident in cortical bone, with a significant increase in periosteal mineral apposition rate. Importantly, no adverse extra-skeletal side effects were observed through Y1 receptor antagonism over the 8-week treatment period, with no effects of even the higher BIBO3304 dose on body weight, adiposity, energy metabolism or circulating corticosterone levels. Taken together, this work describes the first NPY-based anabolic treatment for improving bone mass, and highlights the therapeutic potential of blocking Y1 receptor signalling for the prevention of, or recovery from, degenerative skeletal diseases. {\textcopyright} 2012 Elsevier Inc.},
	keywords = {animal experiment, animal tissue, Animals, Arginine, article, body weight, Bone Density, bone mass, bone metabolism, bone strength, cancellous bone, controlled study, cortical bone, corticosterone, corticosterone blood level, dose response, Dose-Response Relationship, Drug, drug effect, drug mechanism, drug megadose, energy metabolism, femur, food intake, glucose, glucose homeostasis, Homeostasis, male, Mice, Mice, Inbred C57BL, morphometrics, mouse, n2 diphenylacetyl n (4 ureidomethylbenzyl)argininamide, neuropeptide Y, Neuropeptide yY, nonhuman, obesity, Oral drug administration, osteoblast, osteoclast activity, Osteogenesis, osteolysis, Osteopenia, Receptors, Neuropeptide Y, signal transduction, trabecular bone, Y1 receptor antagonism},
	isbn = {87563282 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859915620\&partnerID=40\&md5=29596d34bad3cbf751d1cbfadb63abb9},
	author = {Sousa, D.M. and Baldock, P.A. and Enriquez, R.F. and Zhang, L. and Sainsbury, A. and Lamghari, M. and Herzog, H.}
}
@article {2797,
	title = {Osteoporotic hip fractures: Bisphosphonates sales and observed turning point in trend. A population-based retrospective study},
	year = {2012},
	month = {2012},
	publisher = {Elsevier},
	author = {Alves, Sandra Maria and Economou, Theodoros and Oliveira, Carla and Ribeiro, Ana Isabel and Neves, Nuno and Gom{\'e}z-Barrena, Enrique and de Pina, Maria de F{\'a}tima}
}
@article {2241,
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	journal = {Plant CellPlant Cell},
	volume = {23},
	year = {2011},
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Riou-Khamlichi, C., Menges, M., Healy, J.M., Murray, J.A.H., Sugar control of the plant cell cycle: Differential regulation of Arabidopsis D-type cyclin gene expression (2000) Mol. Cell. Biol., 20, pp. 4513-4521; 
Sabatini, S., Beis, D., Wolkenfelt, H., Murfett, J., Guilfoyle, T., Malamy, J., Benfey, P., Scheres, B., An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root (1999) Cell, 99, pp. 463-472; 
Schnittger, A., Sch{\"o}binger, U., Bouyer, D., Weinl, C., Stierhof, Y.D., H{\"u}lskamp, M., Ectopic D-type cyclin expression induces not only DNA replication but also cell division in Arabidopsis trichomes (2002) Proc. Natl. Acad. Sci. USA, 99, pp. 6410-6415; 
van Leene, J., Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana (2010) Mol. Syst. Biol., 6, p. 397; 
Vanneste, S., Cell cycle progression in the pericycle is not sufficient for SOLITARY ROOT/IAA14-mediated lateral root initiation in Arabidopsis thaliana (2005) Plant Cell, 17, pp. 3035-3050; 
Verkest, A., Manes, C.L., Vercruysse, S., Maes, S., van der Schueren, E., Beeckman, T., Genschik, P., De Veylder, L., The cyclin-dependent kinase inhibitor KRP2 controls the onset of the endoreduplication cycle during Arabidopsis leaf development through inhibition of mitotic CDKA;1 kinase complexes (2005) Plant Cell, 17, pp. 1723-1736; 
Verkest, A., Weinl, C., Inz{\'e}, D., De Veylder, L., Schnittger, A., Switching the cell cycle. Kip-related proteins in plant cell cycle control (2005) Plant Physiol., 139, pp. 1099-1106; 
Wang, H., Qi, Q., Schorr, P., Cutler, A.J., Crosby, W.L., Fowke, L.C., ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid (1998) Plant J., 15, pp. 501-510; 
Wildwater, M., Campilho, A., Perez-Perez, J.M., Heidstra, R., Blilou, I., Korthout, H., Chatterjee, J., Scheres, B., The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots (2005) Cell, 123, pp. 1337-1349; 
Willemse, J., Kulikova, O., de Jong, H., Bisseling, T., A new whole-mount DNA quantification method and the analysis of nuclear DNA content in the stem-cell niche of Arabidopsis roots (2008) Plant J., 55, pp. 886-894; 
Winter, D., Vinegar, B., Nahal, H., Ammar, R., Wilson, G.V., Provart, N.J., An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets (2007) PLoS ONE, 2, pp. e718; 
Zhou, Y., Fowke, L.C., Wang, H., Plant CDK inhibitors: Studies of interactions with cell cycle regulators in the yeast twohybrid system and functional comparisons in transgenic Arabidopsis plants (2002) Plant Cell Rep., 20, pp. 967-975; 
Zhou, Y., Niu, H., Brandizzi, F., Fowke, L.C., Wang, H., Molecular control of nuclear and subnuclear targeting of the plant CDK inhibitor ICK1 and ICK1-mediated nuclear transport of CDKA (2006) Plant Mol. Biol., 62, pp. 261-278; 
Zhou, Y., Wang, H., Gilmer, S., Whitwill, S., Fowke, L.C., Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana (2003) Planta, 216, pp. 604-613
},
	month = {2011///},
	pages = {641 - 660},
	abstract = {The integration of cell division in root growth and development requires mediation of developmental and physiological signals through regulation of cyclin-dependent kinase activity. Cells within the pericycle form de novo lateral root meristems, and D-type cyclins (CYCD), as regulators of the G                        1-to-S phase cell cycle transition, are anticipated to play a role. Here, we show that the D-type cyclin protein CYCD2;1 is nuclear in Arabidopsis thaliana root cells, with the highest concentration in apical and lateral meristems. Loss of CYCD2;1 has a marginal effect on unstimulated lateral root density, but CYCD2;1 is rate-limiting for the response to low levels of exogenous auxin. However, while CYCD2;1 expression requires sucrose, it does not respond to auxin. The protein Inhibitor-Interactor of CDK/Kip Related Protein2 (ICK2/KRP2), which interacts with CYCD2;1, inhibits lateral root formation, and ick2/krp2 mutants show increased lateral root density. ICK2/ KRP2 can modulate the nuclear levels of CYCD2;1, and since auxin reduces ICK2/KRP2 protein levels, it affects both activity and cellular distribution of CYCD2;1. Hence, as ICK2/KRP2 levels decrease, the increase in lateral root density depends on CYCD2;1, irrespective of ICK2/CYCD2;1 nuclear localization. We propose that ICK2/KRP2 restrains root ramification by maintaining CYCD2;1 inactive and that this modulates pericycle responses to auxin fluctuations. {\textcopyright} 2011 American Society of Plant Biologists.
},
	keywords = {Arabidopsis, Arabidopsis protein, Arabidopsis Proteins, Arabidopsis thaliana, article, ATP dependent 26S protease, cell cycle, cell cycle protein, Cell Cycle Proteins, cell nucleus, CycD2 protein, Arabidopsis, cycline, Cyclins, gene expression regulation, Gene Expression Regulation, Plant, genetics, growth, development and aging, ICK2 protein, Arabidopsis, indoleacetic acid derivative, Indoleacetic Acids, metabolism, mutation, plant root, Plant roots, proteasome, Proteasome Endopeptidase Complex},
	isbn = {10404651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953123085\&partnerID=40\&md5=f1355f64db2bcf9f2d881104f23005eb},
	author = {Sanz, L. and Dewitte, W. and Forzani, C. and Patell, F. and Nieuwland, J. and Wen, B. and Quelhas, P. and de Jager, S. and Titmus, C. and Campilho, A. and Ren, H. and Estelle, M. and Wang, H. and Murray, J.A.H.}
}
@book {2224,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6669 LNCS},
	year = {2011},
	note = {Conference code: 85429Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21257-4_19Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: meb09026@fe.up.ptReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Alattar, M., Osman, N., Fahmy, A., Myocardial segmentation using constrained multi-seeded region growing (2010) LNCS, 6112, pp. 89-98. , Campilho, A., Kamel, M. (eds.) ICIAR 2010 Springer, Heidelberg; 
Wu, Q., Merchant, F., Castleman, K., (1996) Microscope Image Processing, , ch. 7. Elsevier, Amsterdam; 
Gonzalez, R., Woods, R., Eddins, S., (2004) Digital Image Processing Using MATLAB, , ch. 9. Pearson Education, London; 
Sharma, N., Aggarwal, L., Automated medical image segmentation techniques (2010) Journal of Medical Physics, 35 (1), pp. 3-14; 
Pham, D., Xu, C., A survey of current methods in medical image segmentation (1998) Annual Review of Biomedical Engineering, 2, pp. 315-338; 
Hamarneh, G., Li, X., Watershed segmentation using prior shape and appearance knowledge (2009) Image and Vision Comp, 27 (1-2), pp. 59-68; 
Khadir, S., Ahamed, R., Moving toward region-based image segmentation techniques: A study (2009) Journal of Theoretical and Applied Information Technology, 5 (1), pp. 1-7; 
Ahmed, M., Mohamad, D., Segmentation of brain mr images for tumor extraction by combining kmeans clustering and perona-malik anisotropic diffusion model (2010) International Journal of Image Processing, 2 (1), pp. 1-8; 
Mayer, A., Greenspan, H., An adaptive mean-shift framework for MRI brain segmentation (2009) IEEE Trans. on Medical Imaging, 28 (8), pp. 1-12; 
Dabov, K., Foi, A., Katkovnik, V., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 1-16; 
Otsu, N., A threshold selection method from gray level histograms (1979) IEEE Trans. Systems, Man and Cybernetics, 9 (1), pp. 62-66; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Computers in Biology and Medicine, 39 (9), pp. 1-9; 
Bleau, A., Leon, J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370
},
	month = {2011///},
	pages = {151 - 158},
	address = {Las Palmas de Gran Canaria},
	abstract = {Rodent models of myocardial infarction (MI) have been extensively used in biomedical research towards the implementation of novel regenerative therapies. Permanent ligation of the left anterior descending (LAD) coronary artery is a commonly used method for inducing MI both in rat and mouse. Post-mortem evaluation of the heart, particularly the MI extension assessment performed on histological sections, is a critical parameter for this experimental setting. MI extension, which is defined as the percentage of the left ventricle affected by the coronary occlusion, has to be estimated by identifying the infarcted- and the normal-tissue in each section. However, because it is a manual procedure it is time-consuming, arduous and prone to bias. Herein, we introduce semi-automatic and automatic approaches to perform segmentation which is then used to obtain the infarct extension measurement. Experimental validation is performed comparing the proposed approaches with manual annotation and a total error not exceeding 8\% is reported in all cases. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Extension evaluations, Image analysis, Image segmentation, Infarct extension evaluation, k-means, Mammals, meanshift, otsu, Pattern recognition, region growing, Tissue, watershed},
	isbn = {03029743 (ISSN); 9783642212567 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=360bb21a8c1c3a5fdd394c647fcadbbc},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@book {2818,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)},
	volume = {6669 LNCS},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: Scopus},
	month = {2011///},
	pages = {151 - 158},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=58726bd7fc58cf587a13af9e3ba0c151},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@article {2815,
	title = {MIQuant - semi-automation of infarct size assessment in models of cardiac ischemic injury},
	journal = {PLoS ONE},
	volume = {6},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: ScopusArt. No.: e25045},
	month = {2011///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80053583980\&partnerID=40\&md5=757f7f25dcffa46f5fd66ccee74f13f0},
	author = {Nascimento, D.S. and Valente, M. and Esteves, T. and De F{\'a}tima De Pina, M. and Guedes, J.G. and Freire, A. and Quelhas, P. and Pinto-Do-{\'O}, P.}
}
@article {2231,
	title = {Molecular recognition force spectroscopy: A new tool to tailor targeted nanoparticles},
	journal = {SmallSmall},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1002/smll.201002074Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis̃o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Verma, I.M., Weitzman, M.D., (2005) Annu. Rev. Biochem., 74, pp. 711-738;Midoux, P., Breuzard, G., Gomez, J.P., Pichon, C., (2008) Curr. Gene Ther., 8, pp. 335-352; 
Oliveira, H., Fernandez, R., Pires, L.R., Martins, M.C., Simoes, S., Barbosa, M.A., Pego, A.P., (2010) J. Controlled Release, 143, pp. 350-358; 
Hinterdorfer, P., Baumgartner, W., Gruber, H.J., Schilcher, K., Schindler, H., (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 3477-3481; 
Ebner, A., Wildling, L., Kamruzzahan, A.S., Rankl, C., Wruss, J., Hahn, C.D., Holzl, M., Gruber, H.J., (2007) Bioconjugate Chem., 18, pp. 1176-1184; 
Fotinou, C., Emsley, P., Black, I., Ando, H., Ishida, H., Kiso, M., Sinha, K.A., Isaacs, N.W., (2001) J. Biol. Chem., 276, pp. 32274-32281; 
Ebner, A., Hinterdorfer, P., Gruber, H.J., (2007) Ultramicroscopy, 107, pp. 922-927; 
Baumgartner, W., Hinterdorfer, P., Schindler, H., (2000) Ultramicroscopy, 82, pp. 85-95; 
Evans, E., Ritchie, K., (1997) Biophys. J., 72, pp. 1541-1555; 
Evans, E., Ritchie, K., (1999) Biophys. J., 76, pp. 2439-2447; 
Bell, G.I., (1978) Science, 200, pp. 618-627; 
Sinha, K., Box, M., Lalli, G., Schiavo, G., Schneider, H., Groves, M., Siligardi, G., Fairweather, N., (2000) Mol. Microbiol., 37, pp. 1041-1051; 
Halpern, J.L., Loftus, A., (1993) J. Biol. Chem., 268, pp. 11188-11192; 
Owens III, D.E., Peppas, N.A., (2006) Int. J. Pharm., 307, pp. 93-102; 
Storm, G., Belliot, S.O., Daemen, T., Lasic, D.D., (1995) Adv. Drug Delivery Rev., 17, pp. 31-48; 
Gu, F., Zhang, L., Teply, B.A., Mann, N., Wang, A., Radovic-Moreno, A.F., Langer, R., Farokhzad, O.C., (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 2586-2591; 
Butt, H.J., Jaschke, M., (1995) Nanotechnology, 6, pp. 1-7; 
Hutter, J.L., Bechhoefer, J., (1993) Rev. Sci. Instrum., 64, pp. 1868-1873; 
Rankl, C., Kienberger, F., Wildling, L., Wruss, J., Gruber, H.J., Blaas, D., Hinterdorfer, P., (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 17778-17783
},
	month = {2011///},
	pages = {1236 - 1241},
	abstract = {The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine. A novel screening tool is described to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. The unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. {\textcopyright} 2011 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {article, Atomic force microscopy, Atomic spectroscopy, atomic-force microscopy, chemistry, Event probability, Force spectroscopy, Functionalized nanoparticles, gene technology, Gene-delivery systems, Genes, Medical nanotechnology, medicine, methodology, Microscopy, Atomic Force, Molecular biology, Molecular recognition, molecular recognition force spectroscopy, Nanomedicines, nanoparticle, Nanoparticle formulation, nanoparticles, Nanotechnology, Neuronal cell, Optimization, Receptor interaction, Screening tool, Specific interaction, Targeted nanoparticle},
	isbn = {16136810 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955561627\&partnerID=40\&md5=85fa523edfcd7a6e171a53820be7f230},
	author = {Oliveira, H. and Rangl, M. and Ebner, A. and Mayer, B. and Hinterdorfer, P. and P{\^e}go, A.P.}
}
@article {2287,
	title = {Anti-adhesion and antiproliferative cellulose triacetate membrane for prevention of biomaterial-centred infections associated with Staphylococcus epidermidis},
	journal = {International Journal of Antimicrobial AgentsInt. J. Antimicrob. Agents},
	volume = {35},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: IAAGEdoi: 10.1016/j.ijantimicag.2009.09.017PubMed ID: 19942411Language of Original Document: EnglishCorrespondence Address: Extremina, C.I.; REQUIMTE, Faculdade de Farm{\'a}cia, Universidade do Porto, Rua An{\'\i}bal Cunha 164, 4050-047 Porto, Portugal; email: extremina@gmail.comChemicals/CAS: cellulose triacetate, 9012-09-3; cilastatin plus imipenem, 92309-29-0; Anti-Bacterial Agents; Biocompatible Materials; Cellulose, 9004-34-6; Imipenem, 74431-23-5; cellulose triacetate, 9012-09-3Tradenames: tienam, Merck Sharp and Dohme, GermanyManufacturers: Eastman Kodak, Netherlands; Merck Sharp and Dohme, GermanyReferences: Darouiche, R.O., Treatment of infections associated with surgical implants (2004) N Engl J Med, 350, pp. 1422-1429;Fonseca, A.P., Extremina, C., Fonseca, A.F., Sousa, J.C., Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa (2004) J Med Microbiol, 53, pp. 903-910; 
Donelli, G., Francolini, I., Piozzi, A., Di Rosa, R., Marconi, W., New polymer-antibiotic systems to inhibit bacterial biofilm formation: a suitable approach to prevent central venous catheter-associated infections (2002) J Chemother, 14, pp. 501-507; 
Akiyama, H., Okamoto, S., Prophylaxis of indwelling urethral catheter infection: clinical experience with a modified Foley catheter and drainage system (1979) J Urol, 121, pp. 40-42; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., Cellulose-based biomaterials for bone regeneration: hemi-synthesis and characterisation (1996) Proceedings of polymers in medicine and surgery-PIMS{\textquoteright}96, pp. 73-80. , Glasgow, UK; 
Elam, J.H., Karlsson, C.H., Nygren, H., Pre-adsorption of cellulose ether onto polymer surfaces: adsorption of adhesins and platelet activation (1992) Biomaterials, 14, pp. 233-237; 
Fonseca, A.P., Granja, P.L., Nogueira, J.A., Oliveira, D.R., Barbosa, M.A., Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives (2001) J Mater Sci Mater Med, 12, pp. 543-548; 
Performance standards for antimicrobial susceptibility testing (2006) Sixteenth informational supplement. Document M100-S16. Wayne, PA: CLSI, , Clinical and Laboratory Standards Institute; 
Vacheethasanee, K., Temenoff, J.S., Higashi, J.M., Gary, A., Anderson, J.M., Bayston, R., Bacterial surface properties of clinically isolated Staphylococcus epidermidis strains determine adhesion on polyethylene (1998) J Biomed Mater Res, 42, pp. 425-432; 
Bhat, N.V., Wavhal, D.S., Preparation of cellulose triacetate pervaporation membrane by ammonia plasma treatment (2000) J Appl Polym Sci, 76, pp. 258-265; 
Amaral, I.F., Granja, P.L., Melo Luis, V., Saramago, B., Barbosa, M.A., Functionalization of chitosan membranes through phosphorylation: atomic force microscopy, wettability, and cytotoxicity studies (2006) J Appl Polym Sci, 102, pp. 276-284; 
Thomas, M.J.K., Ando, D.J., (1996) Ultraviolet and visible spectroscopy: analytical chemistry by open learning, , Wiley, New York, NY; 
(1992) High resolution XPS of organic polymers: the Scienta ESCA300 database, , Beamson G., and Briggs D. (Eds), John Wiley \& Sons Ltd, Chichester, UK; 
Yigong Ge, Wikler, M.A., Sahm, D.F., Blosser-Middleton, R.S., Karlowsky, J.A., In vitro antimicrobial activity of doripenem, a new carbapenem (2004) Antimicrob Agents Chemother, 48, pp. 1384-1396; 
Mart{\'\i}nez-Mart{\'\i}nez, L., Pascual, A., Giglio, M.I., Perea, E.J., Effect of subinhibitory concentrations of β-lactams on the production of slime, surface hydrophobicity and adhesion of Staphylococcus epidermidis [in Spanish] (1991) Enferm Infecc Microbiol Clin, 9, pp. 543-546
},
	month = {2010///},
	pages = {164 - 168},
	abstract = {The initial step in preventing biomaterial-associated infections consists of preventing bacterial adhesion to the device surface. One possible approach is the design of antibiotic-releasing biomaterials. Cellulose triacetate (CTA) membranes with the antibiotic imipenem (IPM) entrapped (CTA-IPM) were prepared. The material was characterised in terms of surface morphology by scanning electron microscopy, surface free energy of interaction and X-ray photoelectron spectroscopy (XPS). Antibiotic release studies were also performed. In vitro adhesion of Staphylococcus epidermidis RP62A to CTA-IPM was investigated using a modified microtitre plate assay, and the antibacterial activity of the CTA-IPM membrane was assessed by a modified Kirby-Bauer test, which showed effective entrapment of the antibiotic as confirmed by XPS and hydrophilicity assays. Release studies showed that this drug-polymer conjugate serves as an adequate reservoir for sustained release of IPM over a period of 71 h at an effective bacteriostatic concentration. Moreover, bacterial adhesion tests showed a statistically significant decrease in the adhesion of S. epidermidis RP62A to CTA-IPM compared with its adhesion to CTA alone. The present innovative approach is capable of providing a membrane with anti-adhesive and antiproliferative properties, thus encouraging in vivo studies to provide a better simulation of the clinical situation. {\textcopyright} 2009 Elsevier B.V. and the International Society of Chemotherapy.},
	keywords = {Adhesion, Anti-Bacterial Agents, antibacterial activity, Antimicrobial agents, article, Bacterial adhesion, bacterial growth, bacteriostasis, bacterium adherence, Biocompatible Materials, biomaterial, Cellulose, Cellulose triacetate, cilastatin plus imipenem, controlled study, device infection, drug screening, Equipment and Supplies, growth inhibition, Humans, hydrophilicity, Imipenem, in vitro study, infection prevention, Membranes, Microbial Sensitivity Tests, microtiter plate assay, nonhuman, priority journal, Prosthesis-Related Infections, Scanning electron microscopy, Staphylococcus epidermidis, surface property, sustained drug release, X ray photoelectron spectroscopy},
	isbn = {09248579 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-72649093658\&partnerID=40\&md5=3fbe3d7e15447e7ae40bb5fdd3f63b61},
	author = {Extremina, C.I. and Fonseca, A.F.d. and Granja, P.L. and Fonseca, A.P.}
}
@article {2270,
	title = {Ceramics for dental restorations - an introduction},
	journal = {Una perspectiva de las cer{\'a}micas para restauraciones dentalesDYNADYNA},
	volume = {77},
	year = {2010},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: English; SpanishCorrespondence Address: Santander, S. A.; Universidad Pontificia Bolivariana and Universidad Cooperativa de Colombia, Medell{\'\i}n, Colombia; email: santiago.arango75@gmail.comReferences: Rosenblum, M.A., Schulman, A., A review of allceramic restorations (1997) J Am Dent Assoc, 128 (3), pp. 297-307;Rizkalla, A.S., Jones, D.W., Mechanical properties of commercial high strength ceramic core materials (2004) Dent Mater, 20 (2), pp. 207-212; 
Rizkalla, A.S., Jones, D.W., Indentation fracture toughness and dynamic elastic moduli for commercial feldspathic dental porcelain materials (2004) Dent Mater, 20 (2), pp. 198-206; 
Jones, D.W., Development of dental ceramics. An historical perspective (1985) Dent Clin North Am, 29 (4), pp. 621-644; 
Kelly, J.R., Nishimura, I., Campbell, S.D., Ceramics in dentistry: Historical roots and current perspectives (1996) J Prosthet Dent, 75 (1), pp. 18-32; 
Stejskal, V.D., Metalspecific lymphocytes: Biomarkers of sensitivity in man (1999) Neuro Endocrinol Lett, 20 (5), pp. 289-298; 
Arvidson, K., Wroblewski, R., Migration of metallic ions from screwposts into dentin and surrounding tissues (1978) Scand J Dent Res, 86 (3), pp. 200-205; 
Venclikova, Z., Metallic pigmentation of human teeth and gingiva: Morphological and immunological aspects (2007) Dent Mater J, 26 (1), pp. 96-104; 
Bumgardner, J.D., Lucas, L.C., Cellular response to metallic ions released from nickelchromium dental alloys (1995) J Dent Res, 74 (8), pp. 15-217; 
Mehulic, K., The release of metal ions in the gingival fluid of prosthodontic patients (2005) Acta Stomatol Croat, 39 (1), pp. 47-51; 
Mart{\'\i}nez, F., Cer{\'a}micas dentales: Clasificaci{\'o}n y criterios de selecci{\'o}n (2007) RCOE, 12 (4), pp. 253-263; 
Weinstein, M., Weinstein, L., Weinstein, A., (1962) Porcelain Coveredmetal Reinforced Teeth; 
Anusavice, K.J., Degradability of dental ceramics (1992) Adv Dent Res, 6, p. 829; 
Knorpp, E.A., (1991) Control Arrangement for Dental Furnaces, Especially Microprocessorcontrolled Preheating Furnaces; 
Anusavice, K.J., (2004) Phillips Ciencia De Los Materiales Dentales, , Und{\'e}cima ed: Elsevier Espa{\~n}a; 
Meyer, J.M., O{\textquoteright}Brien, W.J., Yu, C.U., Sintering of dental porcelain enamels (1976) J Dent Res, 55 (4), pp. 696-699; 
Denry, I.L., Recent advances in ceramics for dentistry (1996) Crit Rev Oral Biol Med, 7 (2), pp. 134-143; 
Campbell, S.D., Dimensional and formation analysis of a restorative ceramic and how it works (1995) J Prosthet Dent, 74 (4), pp. 332-340; 
Conrad, H.J., Seong, W.J., Pesun, I.J., Current ceramic materials and systems with clinical recommendations: A systematic review (2007) J Prosthet Dent, 98 (5), pp. 389-404; 
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},
	month = {2010///},
	pages = {26 - 36},
	abstract = {Dental ceramics are the preferred materials for oral restoration due to some characteristics, such as adequate esthetics, high fracture strength and chemical stability. Currently, dental professionals have a large amount of ceramic systems to choose from, all of them having small differences regarding their chemistry, processing temperatures, mechanical strength and clinical applications. These differences lead to classification systems that are difficult to understand by professionals outside the dental field. The aim of this work is to review the current dental ceramic systems and present them from a compositional perspective to assure the comprehension of these materials by professionals who belong to the Biomedical Engineering field. A great effort was made to avoid classification methods imposed by manufacturers and to obtain a quick compilation of available information from the literature allowing the reader to have a general view of the stateoftheart on dental ceramics.},
	keywords = {Ceramic systems, Feldspathic porcelain, Glassceramics, Leucite, Mechanical and physical properties, Zirconium oxide},
	isbn = {00127353 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80051564798\&partnerID=40\&md5=2e79174a5238798dfcce9d2b5d7f29e0},
	author = {Santander, S.A. and Vargas, A.P. and Escobar, J.S. and Monteiro, F.J. and Tamayo, L.F.R.}
}
@article {2322,
	title = {Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement},
	journal = {Proceedings of the National Academy of Sciences of the United States of AmericaProc. Natl. Acad. Sci. U. S. A.},
	volume = {106},
	year = {2009},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: PNASAdoi: 10.1073/pnas.0808932106PubMed ID: 19126683Language of Original Document: EnglishCorrespondence Address: Mooney, D. J.; School of Engineering and Applied Sciences, Harvard University, 40 Oxford Street, Cambridge, MA 02138, United States; email: mooneyd@seas.harvard.eduChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; interleukin 8, 114308-91-7; vasculotropin, 127464-60-2; Hydrogels; Integrins; Interleukin-8; Vascular Endothelial Growth Factor AReferences: Debnath, J., Brugge, J.S., Modelling glandular epithelial cancers in 3-dimensional cultures (2005) Nat Rev Cancer, 5, pp. 675-688;Wang, F., Reciprocal interactions between β1-integrin and epidermal growth factor receptor in 3-dimensional basement membrane breast cultures: A different perspective in epithelial biology (1998) Proc Natl Acad Sci USA, 95, pp. 14821-14826; 
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Fischbach, C., Mooney, D., Polymeric systems for bioinspired delivery of angiogenic molecules (2006) Adv Polym Sci, 203, pp. 191-221; 
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Richardson, T.P., Peters, M.C., Ennett, A.B., Mooney, D.J., Polymeric system for dual growth factor delivery (2001) Nat Biotechnol, 19, pp. 1029-1034
},
	month = {2009///},
	pages = {399 - 404},
	abstract = {Three-dimensional culture alters cancer cell signaling; however, the underlying mechanisms and importance of these changes on tumor vascularization remain unclear. A hydrogel system was used to examine the role of the transition from 2D to 3D culture, with and without integrin engagement, on cancer cell angiogenic capability. Three-dimensional culture recreated tumor microenvironmental cues and led to enhanced interleukin 8 (IL-8) secretion that depended on integrin engagement with adhesion peptides coupled to the polymer. In contrast, vascular endothelial growth factor (VEGF) secretion was unaffected by 3D culture with or without substrate adhesion. IL-8 diffused greater distances and was present in higher concentrations in the systemic circulation, relative to VEGF. Implantation of a polymeric IL-8 delivery system into GFP bone marrow-transplanted mice revealed that localized IL-8 up-regulation was critical to both the local and systemic control of tumor vascularization in vivo. In summary, 3D integrin engagement within tumor microenvironments regulates cancer cell angiogenic signaling, and controlled local and systemic blockade of both IL-8 and VEGF signaling may improve antiangiogenic therapies. {\textcopyright} 2009 by The National Academy of Sciences of the USA.},
	keywords = {animal experiment, animal model, Animals, arginylglycylaspartic acid, article, bone marrow transplantation, cancer cell, cancer cell culture, Cell Culture Techniques, concentration (parameters), controlled study, cytokine release, Diffusion, Drug delivery, ECM, Humans, hydrogel, Hydrogels, IL-8, implantation, in vivo study, integrin, Integrins, interleukin 8, Interleukin-8, Mice, microenvironment, Models, Biological, mouse, Mus, Neoplasms, Neovascularization, Pathologic, nonhuman, polymer, priority journal, signal transduction, systemic circulation, Tumor vascularization, upregulation, Vascular Endothelial Growth Factor A, vasculotropin},
	isbn = {00278424 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849121351\&partnerID=40\&md5=acc07042d6f5801f7b704a3f355917cf},
	author = {Fischbach, C. and Hyun, J.K. and Hsiong, S.X. and Evangelista, M.B. and Yuen, W. and Mooney, D.J.}
}
@book {2331,
	title = {The influence of the risk functional in data classification with MLPs},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5163 LNCS},
	year = {2008},
	note = {Conference code: 73798Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-540-87536-9_20Language of Original Document: EnglishCorrespondence Address: Silva, L. M.; INEB -Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUP, Rua Dr. Roberto Frias, 4200 - 465 Porto, Portugal; email: lmsilva@fe.up.ptReferences: Silva, L.M., Alexandre, L.A., Marques de S{\'a}, J.: Data classification with multilayer perceptrons using a generalized error function. Neural Networks (2008) doi:10.1016/j.neunet.2008.04,004Erdogmus, D., Principe, J.C., Generalized information potential criterion for adaptive system training (2002) IEEE Transactions on Neural Networks, 13 (5), pp. 1035-1044;Erdogmus, D., Principe, J.C., An Error-Entropy Minimization Algorithm for Supervised Training of Nonlinear Adaptive Systems (2002) IEEE Transactions on Signal Processing, 50 (7), pp. 1780-1786; 
Santos, J.M., Alexandre, L.A., Marques de S{\'a}, J., The Error Entropy Minimization Algorithm for Neural Network Classification (2004) Int. Conf. on Recent Advances in Soft Computing, , Nottingham, United Kingdom; 
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Marques de S{\'a}, J., (2007) Using SPSS, STATISTICA, MATLAB and R, , 2nd edn. Springer, Heidelberg; 
Embrechts, M.J., Szymanski, B., Sternickel, M., Introduction to Scientific Data Mining: Direct Kernel Methods and Applications (2004) Computationally Intelligent Hybrid Systems, pp. 317-363. , ch. 10, pp, Wiley Interscience, Chichester; 
Bennett, K.P., Embrechts, M.J., An Optimization Perspective on Kernel Partial Least Squares Regression (2003) Models and Applications. NATO Science Series, 3, pp. 227-249. , Advances in Learning Theory: Methods, 190, pp, IOS Press, Amsterdam; 
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Suykens, J.A.K., Gestel, T.V., Brabanter, J.D., Moor, B.D., Vandewalle, J., (2003) Least Squares Support Vector Machines, , World Scientific Pub. Co, Singapore; 
Keerthi, S.S., Shevade, S.K., SMO algorithm for least squares SVM formulations (2003) Neural Computation, 15, pp. 487-507; 
Cristianini, N., Shawe-Taylor, J., (2000) Support Vector Machines and Other KernelBased Learning Methods, , Cambridge University Press, Cambridge; 
Vapnik, V., (1998) Statistical Learning Theory, , Wiley-Interscience, Chichester; 
Sch{\"o}lkopf, B., Smola, A.J., (2002) Learning with Kernels, , MIT Press, Cambridge
},
	month = {2008///},
	pages = {185 - 194},
	address = {Prague},
	abstract = {We investigate the capability of multilayer perceptrons using specific risk functionals attaining the minimum probability of error (optimal performance) achievable by the class of mappings implemented by the multilayer perceptron (MLP). For that purpose we have carried out a large set of experiments using different risk functionals and datasets. The experiments were rigorously controlled so that any performance difference could only be attributed to the different risk functional being used. Statistical analysis was also conducted in a careful way. From the several conclusions that can be drawn from our experimental results it is worth to emphasize that a risk functional based on a specially tuned exponentially weighted distance attained the best performance in a large variety of datasets. As to the issue of attaining the minimum probability of error we also carried out classification experiments using non-MLP classifiers that implement complex mappings and are known to provide the best results until this date. These experiments have provided evidence that at least in many cases, by using an adequate risk functional, it will be possible to reach the optimal performance. {\textcopyright} Springer-Verlag Berlin Heidelberg 2008.},
	keywords = {Backpropagation, Complex mapping, Data classification, Data sets, Electric loads, Experiments, Functionals, Minimum probability of error, MLP classifiers, Multi layer perceptron, Multi-layer perceptrons, Multilayer neural networks, Multilayers, Optimal performance, Pattern recognition systems, Probability density function, risks, Statistical analysis, Weighted distance},
	isbn = {03029743 (ISSN); 3540875352 (ISBN); 9783540875352 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849129862\&partnerID=40\&md5=d9d0d90a540a21b191eebbf1956ed6e9},
	author = {Silva, L.M. and Embrechts, M. and Santos, J.M. and De S{\'a}, J.M.}
}
@article {2334,
	title = {Molecular epidemiology of pseudomonas aeruginosa clinical isolates from Portuguese Central Hospital},
	journal = {Folia MicrobiologicaFolia Microbiol.},
	volume = {53},
	year = {2008},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: FOMIAdoi: 10.1007/s12223-008-0086-yLanguage of Original Document: EnglishCorrespondence Address: Fonseca, A. P.Rua Roberto Frias s/n, 4200-465 Porto, Portugal; email: apfonseca08@sapo.ptChemicals/CAS: DNA, BacterialReferences: Bergmans, D., Bonten, M., van tiel, F., Gaillard, C., London, N., van der Geest, S., de Leeuw, P., Stobbrringh, E., Value of phenotyping methods as an initial screening of Pseudomonas aeruginosa in epidemiologic studies (1997) Infection, 25, pp. 350-354;Bertrand, X., Thouverez, M., Talon, D., Boillot, A., Capellier, G., Floriot, C., Helias, J.P., Endemicity, molecular diversity and colonization routes of Pseudomonas aeruginosa in intensive care units (2001) Intens.Care Med., 27, pp. 1263-1268; 
Br{\'\i}zio, A., Silva, A., Canas, E., Ferreira, T., Lito, L.M., Cristino, J.M., Salgado, M.J., Duarte, A., Spread of ln58 containing bla VIM-2 among Pseudomonas aeruginosa (2006) Clin.Microbiol.Infect.Dis., 12 (SUPPL. 4), p. 931; 
Br{\'\i}zio, A., Vasco, S., Gon{\c c}alves, A.R., Lito, L.M., Cristino, J.M., Salgado, M.J., Duarte, A., Survey of extended-spectrum β-lactamases in Escherichia coli isolates from a Portuguese hospital and characterisation of a novel class 1 integron (In60A) carrying the blaCTX-M-9 gene (2006) Internat.J.Antimicrob.Agents, 28, pp. 320-324; 
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Juretschko, S., Labombardi, V.J., Lerner, S.A., Schreckenberger, P.C., Pseudomonas AST Study Group: Accuracies of β-lactam susceptibility test results for Pseudomonas aeruginosa with four automated systems (BD Phoenix, MicroScan WalkAway, Vitek, and Vitek 2) (2007) J.Clin.Microbiol., 45, pp. 1339-1342; 
King, E.O., Ward, M.K., Raney, D.E., Two simple media for the demonstration of pyocyanin and fluorescein (1954) J.Lab.Clin.Med., 44, pp. 301-307; 
Kiska, D.L., Gilligan, P.H., Pseudomonas and Burkholderia (1999) Manual of Clinical Microbiology, pp. 517-525. , in P.R.Murray,E.J.Baron,M.A.Pfaller,F.C.Tenover,R.H.Yolken(Eds) 7th ed. American Society for Microbiology,Washington (DC); 
Morales, G., Wiehlmann, L., Gudowius, P., van Delden, C., T{\"u}mmler, B., Mart{\'\i}nez, J.L., Rojo, F., Structure of Pseudomonas aeruginosa populations analyzed by single nucleotide polymorphism and pulsed-field gel electrophoresis genotyping (2004) J.Bacteriol., 186, pp. 4228-4237; 
Nogueira, J.A., (1995) Pseudomonas Aeruginosa - Agente Nosocomial, , (In Portuguese) PhD Thesis. Faculdade de Medicina, Universidade do Porto, Porto (Portugal); 
Olive, P.B.D., Principles and applications of methods for DNA-based typing of microbial organisms (1999) J.Clin.Microbiol., 37, pp. 1661-1669; 
Pirnay, J.P., de Vos, D., Cochez, C., Bilocq, F., Vanderkelen, A., Zizi, M., Ghysels, B., Cornelis, P., Pseudomonas aeruginosa displays an epidemic population structure (2002) Environ.Microbiol., 4, pp. 898-911; 
Pirnay, J.P., Matthijs, S., Colak, H., Chablain, P., Bilocq, F., Van eldere, J., de Vos, D., Cornelis, P., Global Pseudomonas aeruginosa biodiversity as reflected in a Belgian river (2005) Environ.Microbiol., 7, pp. 969-980; 
Speijer, H., Savelkoulp, P.H.M., Bonten, M.J., Stobberingh, E.E., Tjhie, J.H.T., Application of different genotyping methods for Pseudomonas aeruginosa in a setting of endemicity in an intensive care unit (1999) J.Clin.Microbiol., 37, pp. 3654-3661; 
Tenover, F., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H., Swaminathan, B., Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing (1995) J.Clin. Microbiol., 33, pp. 2233-2239; 
Wiehlmann, L., Wagner, G., Cramer, N., Siebert, B., Gudowius, P., Morales, G., K{\"o}hler, T., T{\"u}mmler, B., Population structure of Pseudomonas aeruginosa (2007) Proc.Nat.Acad.Sci.USA, 104, pp. 8101-8106
},
	month = {2008///},
	pages = {540 - 546},
	abstract = {The relatedness between clinical isolates of P. aeruginosa obtained from patients during their stay in a Portuguese Central Hospital was evaluated. Genotypic fingerprinting (M13-PCR), phenotypic methods (biotyping and antibiotyping) and epidemiological information (spatial and temporal links) were used to evaluate the relatedness between 88 clinical isolates (68 patients), selected randomly out of 189. Sixty-two M13 types were found, 12 of them containing isolates from more than one patient. Thirty-four antibiotypes were found, as well as a significant association (p < 0.05) between epidemic isolates and multiresistance patterns. The nosocomial transmission of P. aeruginosa strains may be limited since M13 typing demonstrated a high degree of diversity among all the isolates, suggesting the occurrence of mainly independent infectious episodes. The results show the possible occurrence of cross-acquisition, cross-colonization and cross-infection and suggest an epidemic population structure for P. aeruginosa in this hospital.},
	keywords = {antibiotic resistance, article, bacterial DNA, cross infection, disease transmission, DNA, Bacterial, Drug Resistance, Microbial, Electrophoresis, Gel, Pulsed-Field, epidemiology, genetics, genotype, Hospitals, Teaching, human, Humans, isolation and purification, microbiology, polymerase chain reaction, Portugal, Pseudomonas aeruginosa, Pseudomonas infection, Pseudomonas Infections, pulsed field gel electrophoresis, Sampling Studies, statistics, teaching hospital},
	isbn = {00155632 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-65549157108\&partnerID=40\&md5=ba6aed0b4b5f511f340fea96df7b6449},
	author = {Fonseca, A.P. and Correia, P. and Extremina, C.I. and Sousa, J.C. and Tenreiro, R. and Barros, H.}
}
@article {2351,
	title = {Surface characterization and cell response of a PLA/CaP glass biodegradable composite material},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {85},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31546PubMed ID: 17729262Language of Original Document: EnglishCorrespondence Address: Ginebra, M. P.; Department of Materials Science, Institute of Bioengineering of Catalonia (IBEC), Technical University of Catalonia, Avda Diagonal 647, 08028-Barcelona, Spain; email: maria.pau.ginebra@upc.eduChemicals/CAS: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, 298-93-1; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; osteocalcin, 136461-80-8; polylactic acid, 26100-51-6; Alkaline Phosphatase, EC 3.1.3.1; Bone Substitutes; calcium phosphate, 10103-46-5; Calcium Phosphates; Lactic Acid, 50-21-5; Osteocalcin, 104982-03-8; poly(lactic acid), 26100-51-6; PolymersReferences: Barrows, T., Degradable implants materials: A review of synthetic absorbable polymers and their applications (1986) Clin Mater, 1, pp. 233-267;Ciccone, W., Motz, C., Bentley, C., Tasto, J., Bioabsorbable implants in orthopaedics: New developments and clinical applications (2001) J Am Acad Orthop Surg, 9, pp. 280-288; 
Dunn, R.L., Clinical applications and update on the poly (alpha-hydroxy acids) (1995) Biomedical Applications of Synthetic Biodegradable Polymers, pp. 17-31. , Hollinger JO, editor, Boca Raton: CRC Press;; 
Middleton, J.C., Tipton, A.J., Synthetic biodegradable polymers as orthopedic devices (2000) Biomaterials, 21, pp. 2335-2346; 
B{\"o}stman O Current concepts review. Absorbable implants for fracture fixation. J Bone Joint Surgery 1991;73A:148-153Ashammakhi, N., Suuronen, R., Tiainen, J., Tormala, P., Waris, T., Spotlight on naturally absorbable osteofixation devices (2003) J Craniofac Surg, 14, pp. 247-259; 
Hou, Q.P., Grijpma, D.W., Feijen, J., Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique (2003) Biomaterials, 24, pp. 1937-1947; 
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Navarro, M., Ginebra, M.P., Planell, J.A., Barrias, C.C., Barbosa, M., In vitro degradation behavior of a novel bioabsorbable composite material based on PLA and a soluble CaP glass (2005) Acta Biomaterialia, 1, pp. 411-419; 
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Beumer, G., van Blitterswijk, C., Bakker, D., Ponec, M., Cell-seeding and in vitro biocompatibility evaluation of polymeric matrices of PEO/PBT copolymers and PLLA (1993) Biomaterials, 14, pp. 598-604; 
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Charles-Harris, M., Navarro, M., Engel, E., Aparicio, C., Ginebra, M.P., Planell, J., A Surface characterisation of completely degradable composite scaffolds (2005) J Mater Svi: Mater Med, 16, pp. 1125-1130; 
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Owen, T.A., Aronow, M., Shalhoub, V., Barone, L.M., Wilming, L., Tassinari, M.S., Progressive development of the rat osteoblast phenotype in vitro - Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular-matrix (1990) J Cell Physiol, 143, pp. 420-430; 
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Quarles, L.D., Yohay, D.A., Lever, L.W., Caton, R., Wenstrup, R.J., Distinct proliferative and differentiated stages of murine Mc3T3-E1 cells in culture - An in vitro model of osteoblast development (1992) J Bone Miner Res, 7, pp. 683-692; 
Boyan, B.D., Dean, D.D., Lohmann, C.H., Cochran, D.L., Sylvia, V.L., Schwartz, Z., The titanium-bone cell interface in vitro: The role of the surface in promoting osteointegration (2001) Titanium in Medicine, pp. 562-585. , Brunette D, Tengvall P, Textor M, Thomsen P, editors, Berlin: Springer-Verlag;; 
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Boyan, B.D., Hummert, T.W., Kieswetter, K., Schraub, D., Dean, D.D., Schwartz, Z., Effect of titanium surface characteristics on chondrocytes and osteoblasts in vitro (1995) Scan Electron Microsc, 5, pp. 323-335; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Effect of titanium surface-roughness on proliferation. Differentiation, and protein-synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401
},
	month = {2008///},
	pages = {477 - 486},
	abstract = {Bioabsorbable materials are of great interest for bone regeneration applications, since they are able to degrade gradually as new tissue is formed. In this work, a fully biodegradable composite material containing polylactic acid (PLA) and calcium phosphate (CaP) soluble glass particles has been characterized in terms of surface properties and cell response. Cell cultures were performed in direct contact with the materials and also with their extracts, and were evaluated using the MTT assay, alkaline phosphatase activity, and osteocalcin measurements. The CaP glass and PLA were used as reference materials. No significant differences were observed in cell proliferation with the extracts containing the degradation by-products of the three materials studied. A relation between the materials wettability and the material-cell interactions at the initial stages of contact was observed. The most hydrophilic material (CaP glass) presented the highest cell adhesion values as well as an earlier differentiation, followed by the PLA/glass material. The incorporation of glass particles into the PLA matrix increased surface roughness. SEM images showed that the heterogeneity of the composite material induced morphological changes in the cells cytoskeleton. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, Absorbable Implants, alkaline phosphatase, article, Biodegradable composite materials, biodegradable implant, Biodegradation, Bone, Bone Regeneration, Bone Substitutes, calcium phosphate, Calcium phosphates, cell adhesion, Cell culture, cell function, cell interaction, Cell Line, cell proliferation, Cell response, Composite materials, cytoskeleton, glass, human, human cell, Humans, hydrophilicity, hydrophobicity, In vitro test, Lactic Acid, materials testing, Osteoblasts, osteocalcin, polylactic acid, Polymers, scanning electron microscope, Surface analysis, Surface Properties, wettability},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-42149187233\&partnerID=40\&md5=8942b2e00b94ff0f723261e1893d04b5},
	author = {Navarro, M. and Engel, E. and Planell, J.A. and Amaral, I. and Barbosa, M. and Ginebra, M.P.}
}
@article {2378,
	title = {Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {81},
	year = {2007},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31073PubMed ID: 17252543Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Ceramics; Durapatite, 1306-06-5Manufacturers: Plasma BiotalReferences: Teixeira S, Queiroz AC, Monteiro FJ, Ferraz MP, Vilar R, Eugenio S. Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results. Bioceramics 2006;18 (Parts 1, 2):309-311, 105-108Fernandez-Pradas, J.M., Cleries, L., Martinez, E., Sardin, G., Esteve, J., Morenza, J.L., Influence of thickness on the properties of hydroxyapatite coatings deposited by KrF laser ablation (2001) Biomaterials, 22, pp. 2171-2175;Fernandez-Pradas, J.M., Cleries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the deposition rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys A: Mater Sci Process, 72, pp. 613-618; 
Foltyn, S.R., Dye, R., Ott, K.C., Peterson, E., Hubbard, K.M., Hutchinson, W., Muenchausen, R.E., Wu, X.D., Target modification in the excimer laser deposition of YBa 2Cu 3O 7-x thin films (1991) Appl Phys Lett, 59, pp. 594-596; 
Khor, K.A., Vreeling, A., Dong, Z.L., Cheang, P., Laser treatment of plasma sprayed HA coatings (1999) Mater Sci Eng A: Struct Mater Prop Microstruct Process, 266 (1-2), pp. 1-7; 
Ricci, J.L., Alexander, H., Laser microtexturing of implant surfaces for enhanced tissue integration (2001) Key Engineering Mater, pp. 198-199,179-202; 
Galli, C., Guizzardi, S., Passeri, G., Martini, D., Tinti, A., Mauro, G., Macaluso, G.M., Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces (2005) J Periodontol, 76, pp. 364-372; 
Oliveira, V., Conde, O., Vilar, R., UV laser micromachining of ceramic materials: Formation of columnar topographies (2001) Adv Eng Mater, 3 (1-2), pp. 75-81; 
Serra, P., FernandezPradas, J., Sardin, G., Morenza, J.L., Interaction effects of an excimer laser beam with hydroxyapatite targets (1997) Appl Surf Sci, 110, pp. 384-388; 
Serra, P., Morenza, J.L., Fluence dependence of hydroxyapatite laser ablation plumes (1998) Thin Solid Films, 335 (1-2), pp. 43-48; 
Duncan, A.C., Weisbuch, F., Rouais, F., Lazare, S., Baquey, C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosens Bioelectron, 17, pp. 413-426; 
Walboomers, X.F., Croes, H.J.E., Ginsel, L.A., Jansen, J.A., Contact guidance of rat fibroblasts on various implant materials (1999) J Biomed Mater Res, 47, pp. 204-212; 
Carvalho, P., Manzanares, M.C., Franch, J., Belmonte, A.M., Lafuente, M.P., Serra, I., Fernandez-Pradas, J.M., Morenza, J.L., Osteointegration of calcium phosphate coatings obtained by pulsed laser deposition (2003) J Dent Res, 82, p. 573; 
Ahmad, M., McCarthy, M., Gronowicz, G., An in vitro model for mineralization of human osteoblast-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220; 
Matsuura, T., Hosokawa, R., Okamoto, K., Kimoto, T., Akagawa, Y., Diverse mechanisms of osteoblast spreading on hydroxyapatite and titanium (2000) Biomaterials, 21, pp. 1121-1127; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Giardino, R., Comparative interspecies investigation on osteoblast cultures: Data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57, pp. 57-62; 
Boyan, B.D., Lossdoerfer, S., Wang, L., Zhao, G., Lohmann, C.H., Cochran, D.L., Schwartz, Z., Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies (2003) Eur Cell Mater, 6, pp. 22-27; 
Anselme, K., Bigerelle, M., Topography effects of pure titanium substrates on human osteoblast long-term adhesion (2005) Acta Biomater, 1, pp. 211-222; 
Anselme, K., Linez, P., Bigerelle, M., Le Maguer, D., Le Maguer, A., Hardouin, P., Hildebrand, H.F., Leroy, J.M., The relative influence of the topography and chemistry of TiAl 6V 4 surfaces on osteoblastic cell behaviour (2000) Biomaterials, 21, pp. 1567-1577; 
Anselme, K., Noel, B., Hardouin, P., Human osteoblast adhesion on titanium alloy, stainless steel, glass and plastic substrates with same surface topography (1999) J Mater Sci: Mater Med, 10, pp. 815-819; 
Cleries L, Fernandez-Pradas JM, Morenza JL. Bone growth on and resorption of calcium phosphate coatings obtained by pulsed laser deposition. J Biomed Mater Res 2000;49:43. Erratum 2001;57:473Deligianni, D.D., Katsala, N., Ladas, S., Sotiropoulou, D., Amedee, J., Missirlis, Y.F., Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption (2001) Biomaterials, 22, pp. 1241-1251; 
dos Santos, E.A., Farina, M., Soares, G.A., Specific proliferation rates of human osteoblasts on calcium phosphate surfaces with variable concentrations of α-TCP (2007) Mater Sci Eng, 27, pp. 61-66; 
Hao, L., Lawrence, J., Laser surface treatment of magnesia partially stabilized zirconia for enhanced human skin fibroblast cell response (2004) J Laser Appl, 16, pp. 55-64; 
Kieswetter, K., Schwartz, Z., Dean, D.D., Boyan, B.D., The role of implant surface characteristics in the healing of bone (1996) Crit Rev Oral Biol Med, 7, pp. 329-345; 
Salda{\~n}a, L., Gonz{\'n}lez-Carrasco, J.L., Rodriguez, M., Munuera, L., Vilaboa, N., Osteoblast response to plasma-spray porous Ti 6AL 4V coating on substrates of identical alloy (2006) J Biomed Mater Res A, 77, pp. 608-617; 
Lincks, J., Boyan, B.D., Blanchard, C.R., Lohmann, C.H., Liu, Y., Cochran, D.L., Dean, D.D., Schwartz, Z., Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition (1998) Biomaterials, 19, pp. 2219-2232; 
Martin, J.Y., Dean, D.D., Cochran, D.L., Simpson, J., Boyan, B.D., Schwartz, Z., Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces (1996) Clin Oral Implants Res, 7, pp. 27-37; 
Noth, U., Hendrich, C., Merklein, F., Altvater, T., Rader, C.P., Schutze, N., Eulert, J., Thull, R., Standardised testing of bone-implant surfaces using an osteoblast cell culture system. II. Titanium surfaces of varying degrees of roughness (1999) Biomedizinische Technik, 44 (1-2), pp. 6-11; 
Ponsonnet, L., Reybier, K., Jaffrezic, N., Comte, V., Lagneau, C., Lissac, M., Martelet, C., Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour (2003) Mater Sci Eng C: Biomimetic Supramolecular Syst, 23, pp. 551-560; 
Ramires, P.A., Romito, A., Cosentino, F., Milella, E., The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour (2001) Biomaterials, 22, pp. 1467-1474; 
Redey, S.A., Nardin, M., Bernache-Assolant, D., Rey, C., Delannoy, P., Sedel, L., Marie, P.J., Behavior of human osteoblastic cells on stoichiometric hydroxyapatite and type A carbonate apatite: Role of surface energy (2000) J Biomed Mater Res, 50, pp. 353-364; 
Rosa, A.L., Beloti, M.M., van Noort, R., Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography (2003) Dent Mater, 19, pp. 768-772; 
Schmidt, C., Ignatius, A.A., Claes, L.E., Proliferation and differentiation parameters of human osteoblasts on titanium and steel surfaces (2001) J Biomed Mater Res, 54, pp. 209-215; 
Schwartz, Z., Kieswetter, K., Dean, D.D., Boyan, B.D., Underlying mechanisms at the bone-surface interface during regeneration (1997) J Periodontal Res, 32, pp. 166-171; 
Schwartz, Z., Martin, J.Y., Dean, D.D., Simpson, J., Cochran, D.L., Boyan, B.D., Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation (1996) J Biomed Mater Res, 30, pp. 145-155; 
Wang, J., de Boer, J., de Groot, K., Preparation and characterization of electrodeposited calcium phosphate/chitosan coating on Ti 6Al 4V plates (2004) J Dent Res, 83, pp. 296-301; 
Xavier, S.P., Carvalho, P.S.P., Beloti, M.M., Rosa, A.L., Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments (2003) J Dent, 31, pp. 173-180
},
	month = {2007///},
	pages = {920 - 929},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture, and microchemistry. The surface topography eventually affects the nature and the intensity of the interactions that occur at biomaterial- biological interface (cell adhesion, mobility, spreading, and proliferation). Therefore, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (in this case, osteoblasts). There are a number of substrates that already have been studied in terms of surface topography; however, few studies are related to hydroxyapatite (HA) substrates. As it is well established, HA is a well-known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense HA substrates was altered by using KFr excimer laser. The surface was characterized by atomic force microscopy and contact angle measurements, while the cell distribution and morphology was assessed by scanning electron microscopy and confocal laser scanning microscopy. Results revealed that the surface is characterized by a homogeneous columnar structure with high specific area. Moreover, cells were able to attach and spread on the surface of the samples, and gradually grow into nearly confluent monolayers. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {article, Atomic force microscopy, Biocompatibility, biomaterial, cell adhesion, Cell Communication, Cell Line, Tumor, cell motility, cell proliferation, cell spreading, cellular distribution, ceramic prosthesis, ceramics, chemistry, confocal laser microscopy, controlled study, Durapatite, Excimer laser, Excimer lasers, human, human cell, Humans, hydroxyapatite, laser, Lasers, material coating, Microchemistry, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Electron, Scanning, Microtexture, monolayer culture, osteoblast, Osteoblasts, reproducibility, Scanning electron microscopy, Surface microtopography, Surface modification, Surface Properties, surface property, Surface topography, Surface treatment, Tissue engineering},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249742544\&partnerID=40\&md5=b6cb0aaa7b8e4a267b97d91d7171862f},
	author = {Teixeira, S. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@article {2376,
	title = {Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix},
	journal = {BiomaterialsBiomaterials},
	volume = {28},
	year = {2007},
	note = {Cited By (since 1996): 39Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2007.04.028PubMed ID: 17532040Language of Original Document: EnglishCorrespondence Address: Evangelista, M.B.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mbe@ineb.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; alkaline phosphatase, 9001-78-9; arginylglycylaspartic acid, 99896-85-2; DNA, 9007-49-2; osteocalcin, 136461-80-8; Alginates; alginic acid, 9005-32-7; Alkaline Phosphatase, EC 3.1.3.1; arginyl-glycyl-aspartic acid, 99896-85-2; Glucuronic Acid, 576-37-4; Hexuronic Acids; Oligopeptides; Osteocalcin, 104982-03-8References: de Vos, P., de Haan, B., Wolters, G.H.J., VanSchilfgaarde, R., Factors influencing the adequacy of microencapsulation of rat pancreatic islets (1996) Transplantation, 62 (7), pp. 888-893;Brissova, M., Lacik, I., Powers, A.C., Anilkumar, A.V., Wang, T., Control and measurement of permeability for design of microcapsule cell delivery system (1998) J Biomed Mater Res, 39 (1), pp. 61-70; 
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Kong, H.J., Kaigler, D., Kim, K., Mooney, D.J., Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution (2004) Biomacromolecules, 5 (5), pp. 1720-1727; 
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Bancroft, J.D., Gamble, M., (2002) Theory and practice of histological techniques. 5th ed, , Elsevier Science, Edinburgh
},
	month = {2007///},
	pages = {3644 - 3655},
	abstract = {There is a need for new therapeutic strategies to treat bone defects caused by trauma, disease or tissue loss. Injectable systems for cell transplantation have the advantage of allowing the use of minimally invasive surgical procedures, and thus for less discomfort to patients. In the present study, it is hypothesized that Arg-Gly-Asp (RGD)-coupled in a binary (low and high molecular weight) injectable alginate composition is able to influence bone cell differentiation in a three-dimensional (3D) structure. Viability, metabolic activity, cytoskeleton organization, ultrastructure and differentiation (alkaline phosphatase (ALP), von Kossa, alizarin red stainings and osteocalcin quantification) of immobilized cells were assessed. Cells within RGD-modified alginate microspheres were able to establish more interactions with the synthetic extracellular matrix as visualized by confocal laser scanning microscope and transmission electron microscopy imaging, and presented a much higher level of differentiation (more intense ALP and mineralization stainings and higher levels of osteocalcin secretion) when compared to cells immobilized within unmodified alginate microspheres. These findings demonstrate that peptides covalently coupled to alginate were efficient in influencing cell behavior within this 3D system, and may provide adequate preparation of osteoblasts for cell transplantation. {\textcopyright} 2007 Elsevier Ltd. All rights reserved.},
	keywords = {3T3 Cells, Alginate, Alginates, alginic acid, alkaline phosphatase, animal cell, Animals, arginylglycylaspartic acid, article, Bone, bone cell, Bone Regeneration, calvaria, cell adhesion, cell differentiation, Cell encapsulation, Cell immobilization, cell metabolism, cell proliferation, cell survival, cell ultrastructure, cell viability, confocal laser microscopy, controlled study, cytology, cytoskeleton, DNA, DNA content, ECM (extracellular matrix), enzyme activity, extracellular matrix, Glucuronic Acid, Hexuronic Acids, hydrogel, immobilized cell, metabolism, Mice, Microscopy, Confocal, Microscopy, Electron, Transmission, microsphere, Microspheres, nonhuman, Oligopeptides, osteoblast, Osteoblasts, osteocalcin, Patient treatment, priority journal, rat, RGD peptide, structure analysis, three dimensional imaging, Tissue engineering, transmission electron microscopy, Transplantation (surgical)},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249870130\&partnerID=40\&md5=4476a4b1ee80320328c1bf37054037bb},
	author = {Evangelista, M.B. and Hsiong, S.X. and Fernandes, R. and Sampaio, P. and Kong, H.-J. and Barrias, C.C. and Salema, R. and Barbosa, M.A. and Mooney, D.J. and Granja, P.L.}
}
@book {2413,
	title = {Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {309-311 I},
	year = {2006},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterlais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Duncan, A.C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosensors and Bioelectronics, 17, pp. 413-426;Walboomers, X.F., Contact guidance of rat fibroblasts on various implant materials (1999) Journal of Biomedical Materials Research, 47, pp. 204-212; 
Queiroz, A.C., Laser surface modifications of hydroxyapatite and glass-reinforced hydroxyapatite (2004) Biomaterials, 25, pp. 4607-4614; 
Ahmad, M., McCarthy, M.B., Gronowicz, G., An in vitro model for mineralization of human osteoblasts-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220
},
	month = {2006///},
	pages = {105 - 108},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture and microchemistry. The surface topography ultimately affects the nature and the strength of the interactions that occur at biomaterial-biological environment (cell adhesion, mobility, spreading and proliferation). Thus, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (for instance, cells). [1] There are a number of substrates that already have been studied (such as silicone, polystyrene, poly-L-lactic acid and titanium coated polystyrene) in terms of surface topography. [2] However, few studies are related to hydroxyapatite substrates. As it is well established, hydroxyapatite is a well known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense hydroxyapatite substrates was altered by using KFr excimer laser. Excimer lasers produce high-intensity, pulsed ultraviolet radiation and are especially well suited for materials processing due to their large beam cross-section area, which permits using mask projection technologies to process relatively large areas in a single step.[3].},
	keywords = {Bone, cell adhesion, Cells, Ceramic materials, Excimer laser, Excimer lasers, hydroxyapatite, Microtexture, Microtopography, morphology, Strength of materials, Surface modification, Surface topography, Surface treatment},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645413875\&partnerID=40\&md5=0234521c5cb850a0ff51ca4c64c39074},
	author = {Teixeira, S. and Queiroz, A.C. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@article {2441,
	title = {Improving the adhesion of poly(ethylene terephthalate) fibers to poly(hydroxyethyl methacrylate) hydrogels by ozone treatment: Surface characterization and pull-out tests},
	journal = {PolymerPolymer},
	volume = {46},
	year = {2005},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: POLMAdoi: 10.1016/j.polymer.2005.08.033Language of Original Document: EnglishCorrespondence Address: Ferreira, L.; Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: lino@mit.eduReferences: Hart, B.L., Deyo, R.A., Cherkin, D.C., (1995) Spine, 20, pp. 11-19;Bao, Q.-B., McCullen, G.M., Higham, P.A., Dumbleton, J.H., Yuan, H.A., (1996) Biomaterials, 17, pp. 1157-1167; 
Martz, E.O., Goel, V.K., Pope, M.H., Park, J.B., (1997) J Biomed Mater Res (Appl Biomater), 38, pp. 267-288; 
Strobel, M., Walzak, M.J., Hill, J.M., Lin, A., Karbashewski, E., Lyons, C.S., (1995) J Adhes Sci Technol, 9 (3), pp. 365-383; 
Le, Q.T., Pireaux, J.J., Caudano, R., (1997) J Adhes Sci Technol, 11, pp. 735-751; 
O{\textquoteright}Hare, L.-A., Smith, J.A., Leadley, S.R., Parbhoo, B., Goodwin, A.J., Watts, J.F., (2002) Surf Interface Anal, 33, pp. 617-625; 
Walzak, M.J., Flynn, S., Foerch, R., Hil, J.M., Karbashewski, E., Lin, A., (1995) J Adhes Sci Technol, 9, pp. 1229-1248; 
Hill, J.M., Karbashewski, E., Lin, A., Strobel, M., Walzak, M.J., (1995) J Adhes Sci Technol, 9 (12), pp. 1575-1591; 
Blais, P., Day, M., Wiles, D.M., (1973) J Appl Polym Sci, 17, pp. 1895-1907; 
Day, M., Wiles, D.M., (1972) J Appl Polym Sci, 16, pp. 175-189; 
Nissen, K.E., Stevens, M.G., Stuart, B.H., Baker, A.T., (2001) J Polym Sci, Part B: Polym Phys, 39, pp. 623-633; 
Chen, W., McCarthy, T.J., (1998) Macromolecules, 31, pp. 3648-3655; 
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Rule, M., Physical constants of poly(oxyethylene-oxyterephthaloyl) (poly(ethylene terephthalate)) (1999) Polymer Handbook, pp. V/113-V/118. , J. Brandrup E.H. Immergut E.A. Grulke Wiley New York; 
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Andrade, J.D., Smith, L.M., Gregonis, D.E., (1985) The Contact Angle and Interface Energetics, , Plenum press New York; 
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Tanaka, K., Minoshima, K., Grela, W., Komai, K., (2002) Compos Sci Technol, 62, pp. 2169-2177; 
Sadiku, E.R., Sanderson, R.D., (2001) Macromol Mater Eng, 286, pp. 535-545; 
Pisanova, E., Zhandarov, S., (2001) J Adhes, 75, pp. 89-127; 
Owens, D.K., (1975) J Appl Polym Sci, 19, pp. 3315-3326; 
Briggs, D., Rance, D.G., Kendall, C.R., Blythe, A.R., (1980) Polymer, 21, pp. 895-900; 
Fu, S.-Y., Yue, C.-Y., Hu, X., Mai, Y.-W., (2000) Compos Sci Technol, 60, pp. 569-579
},
	month = {2005///},
	pages = {9840 - 9850},
	abstract = {This work reports a methodology to improve the adhesion between poly(ethylene terephthalate) (PET) fibers and poly(hydroxyethyl methacrylate) (pHEMA) hydrogels by treating PET with ozone. The surface chemistry of PET was examined by water contact angle measurements, X-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRAS) and attenuated total reflectance infrared spectroscopy (ATR-IR) yielding information about the chemical functionalities at depths upon 0.6 μm. Ozone treatment introduces several polar groups in the surface of PET through oxidation and chain scission resulting in increased wettability. These groups include mostly carboxylic and anhydride groups and in small extent hydroxyl groups. Atomic force microscopy (AFM) analysis shows that the surface of ozone-treated PET films is fully covered with spherical particles that are removed after washing the film with water. During the washing step carboxylic functionalities were removed preferentially, as demonstrated by XPS and IR analysis. According to pull-out tests, PET monofilaments and bundles treated by ozone had a higher adhesion to pHEMA hydrogels than untreated ones. The apparent interfacial shear strength is 65\% higher on pHEMA hydrogel containing an ozonated than an untreated PET monofilament. In addition, the force to pull-out an ozone-treated PET bundle from pHEMA hydrogel is ca. 81\% higher than the one observed for the untreated bundle. {\textcopyright} 2005 Elsevier Ltd. All rights reserved.},
	keywords = {Adhesion, Atomic force microscopy, Contact angle, hydrogel, Hydrogels, infrared spectroscopy, Oxidation, Ozone, ozone treatment, Ozone treatments, Poly(ethylene terephthalate), polyethylene terephthalate fiber, Polyethylene terephthalates, Polymethyl methacrylates, pull-out, Pull-out tests, Surface characterization, Surface chemistry, surface property, X ray photoelectron spectroscopy},
	isbn = {00323861 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-26844540116\&partnerID=40\&md5=bebe6186e9e73c41648eedee7d5c0f61},
	author = {Ferreira, L. and Evangelista, M.B. and Martins, M.C.L. and Granja, P.L. and Esteves, J.L. and Barbosa, M.A.}
}
@book {2478,
	title = {Bonelike{\textregistered}/PLGA hybrid materials for bone regeneration: In vivo evaluation},
	series = {Materials Science ForumMater Sci Forum},
	volume = {455-456},
	year = {2004},
	note = {Conference code: 63332Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: MSFOELanguage of Original Document: EnglishCorrespondence Address: Oliveira, J.M.; Inst. de Engenharia Biom{\'e}dica, Lab. de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jmolive@fe.up.ptReferences: Praemer, A., Furner, S., Rice, D.P., (1992) Am. Acad. Orthop. Surg., p. 1;Glowacki, J., Mulliken, J.B., (1985) Clin. Plast. Surg., 12, p. 233; 
Buck, B.E., Malinin, T.I., Brown, M.D., (1989) Clin. Orthop., 36, p. 129; 
Sepulveda, P., Bressiani, A.H., Bressiani, J.C., Mesenguer, L., K{\"o}nig Jr., B., (2002) J. Biomed. Mater. Res. Vol., 62, p. 587; 
Glimcher, M.J., (1987) Instr. Course Lect., 36, p. 46; 
Yakamuro, T., Hench, L.L., Wilson, J., (1990) CRC Handbook of Bioactive Ceramics, 2, pp. 245-252; 
Klemm, K.W., (1993) Clin. Orthop. \& Rel. Res., 295, pp. 63-76; 
Agrawal, C.M., Ray, R.B., (2001) J. Biomed. Mater. Res., 55, p. 141; 
Santos, J.D., Campbell, J.K., Winton, G.H., (2000), European Patent N{\textdegree} W00068164Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mater. Res., 54, p. 463
},
	month = {2004///},
	pages = {374 - 377},
	address = {Caparica},
	abstract = {The biocompatibility and osteoconductive potential of Bonelike{\textregistered} and Bonelike{\textregistered}/PLGA hybrid materials were evaluated through subcutaneous and tibiae implantations for a 4 week period. Qualitative histology was performed to evaluate new bone formation and characterise bone-implant interface and results showed that Bonelike{\textregistered}/PLGA hybrid materials promote extensive bone growth. After the 4 week implantation period, Bonelike{\textregistered} resorption was recognized, which may be due to the presence of resorbable β and α-tricalcium phosphate phases.},
	keywords = {Bacteria, Biodegradation, Bone, Bonelike resorption, Bonelike{\textregistered}, Histological studies, histology, Hybrid materials, hydroxyapatite, In vivo, physiology, PLGA, Polymers, Surgery, Tissue},
	isbn = {02555476 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17044449739\&partnerID=40\&md5=4d9e5103b703ff0f016854e278f75823},
	author = {Oliveira, J.M. and Kawai, T. and Lopes, M.A. and Ohtsuki, C. and Santos, J.D. and Afonso, A.},
	editor = {Martins R. and Fortunato E. and Ferreira I. and Dias C.}
}
@book {2479,
	title = {Influence of characteristics of the starting hydroxyapatite powders and of deagglomeration procedure, on rheological behaviour of HA suspensions},
	series = {Materials Science ForumMater Sci Forum},
	volume = {455-456},
	year = {2004},
	note = {Conference code: 63332Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: MSFOELanguage of Original Document: EnglishCorrespondence Address: Ferreira, J.M.F.; Dept. of Ceramics Engineering, CICECO, Univ. of Aveiro, 3810-193 Aveiro, Portugal; email: jmf@cv.ua.ptReferences: Kanazawa, T., (1989) Inorganic Phosphate Materials, 52, p. 15. , Materials Science Monographs, (Elsevier Science Publishers, Amsterdam);Elliot, J.C., (1994) Structure and Chemistry of the Apatites and Other Calcium Orthophosphates. Studies in Organic Chemistry, 18. , Elsevier Science, Netherlands; 
Ohgushi, H., Okumura, M., Yoshikawa, T., Inoue, K., Senpuku, N., Tamai, S., Shors, E.C., (1992) J. Biomed. Mater. Res., 26, p. 885; 
Saggio-Woyanski, J., Scott, C., (1992) Am. Ceram. Soc. Bull., 71, p. 1674; 
Lyckfeldt, O., Ferreira, J.M.F., (1998) J. Eur. Ceram. Soc., 18, p. 131; 
Lorenzo, L.M.R., (1999), PhD Thesis MadridOlhero, S.M., Ferreira, J.M.F., (2003) Powder Thecnology, , accepted for publication
},
	month = {2004///},
	pages = {361 - 365},
	address = {Caparica},
	abstract = {In order to evaluate how the characteristics of starting HA powders affect their dispersion ability, several tests were made, including particle size distribution, particle morphology (SEM) and rheological measurements of the suspensions. To enable the preparation of concentrated suspensions as high as possible, the influence of deagglomeration procedure on rheological behaviour of hydroxyapatite aqueous suspensions was evaluated. The results revealed significant differences in the dispersing ability of the powders and on the maximum achievable solids loading. In fact, the maximum solids volume fraction that was possible to incorporate in the initial suspension of HA                        R was only 35-vol.\%, while with the HA
                        S it has been possible to start from 45-vol.\% suspensions. Ball milling revealed to be more efficient than planetary milling in the preparation of HA
                        R suspensions, and enabled to achieve a maximum solids volume fraction of 45-vol.\% at the end of long milling period, which included successive incremental additions of powder. The powder HA
                        S exhibited a much better dispersing ability, enabling the preparation of 60-vol.\% solids only after two hours of planetary milling.
},
	keywords = {Agglomeration, Bioceramics, crystallography, Deagglomeration, Foaming processes, hydroxyapatite, medicine, morphology, Particle size analysis, Powder characteristics, Powders, rheology, Scanning electron microscopy, Sedimentation, Sintering},
	isbn = {02555476 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17044460745\&partnerID=40\&md5=5c72052a267f3f7c544667ce61b5e434},
	author = {Lemos, A.F. and Santos, J.D. and Ferreira, J.M.F.},
	editor = {Martins R. and Fortunato E. and Ferreira I. and Dias C.}
}
@article {2475,
	title = {Laser surface modification of hydroxyapatite and glass-reinforced hydroxyapatite},
	journal = {BiomaterialsBiomaterials},
	volume = {25},
	year = {2004},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2003.11.054PubMed ID: 15120506Language of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB-Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: aqueiroz@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Durapatite, 1306-06-5; Macromolecular SubstancesReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical evaluation of porous and nonporous hydroxyapatite in the treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, pp. 1-10. , I.D.M. MacGregor. Oxford: Oxford University Press; 
Systemic antibiotics in periodontics (1996) J Periodontol, 67, pp. 831-838; 
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Soskolne, W.A., Heasman, H.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis: A multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Prado Da Silva, M.H., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., Porous glass reinforced hydroxyapatite materials produced with different organic additives (2002) J Non-Cryst Solids, 304, pp. 286-292; 
Barinov, S.M., Baschenko, Y.V., Application of ceramic composits as implants: Result and problem (1992) Bioceramics and the Human Body, pp. 206-210. , A. Krajewski. UK: Elsevier Science Publishers; 
Koshino, T., Murase, T., Takagi, T., Saito, T., New bone formation around porous hydroxyapatite wedge implanted in opening wedge high tibial osteotomy in patients with osteoarthritis (2001) Biomaterials, 22, pp. 1579-1582; 
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Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
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Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings (1999) J Biomed Mater Res, 47, pp. 603-611; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P2O5 Glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J Biomed Mater Res, 54, pp. 463-469; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., Adsorption and release of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites (2001) Biomaterials, 22, pp. 1393-1400; 
Cl{\`e}ries, L., Fern{\'a}ndez-Pradas, J.M., Morenza, J.L., Behaviour in simulated body fluid of calcium phosphate coating obtained by laser ablation (2000) Biomaterials, 21, pp. 1861-1865; 
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Fern{\'a}ndez-Pradas, J.M., Cl{\`e}ries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the depositon rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys a, 72, pp. 613-618; 
Zeng, H., Lacefield, W.R., XPS, EDX and FTIR analysis of pulsed laser deposited calcium phosphate bioceramic coatings: The effects of various process parameters (2000) Biomaterials, 21, pp. 23-30; 
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Usoskin, A., Freyhardt, H.C., Krebs, H.U., Influence of light scattering on the development of laser induced ridge-cone structure on target surfaces (1999) Appl Phys a, 69, pp. 823-S826
},
	month = {2004///},
	pages = {4607 - 4614},
	abstract = {Surface treatment of materials with excimer laser radiation often results in the formation of a rough columnar or cone-shaped surface topography, which leads to a considerable increase in the surface area. As a result, the search for a non-porous bioactive material with adequate mechanical properties and a high surface to volume ratio, similar to porous materials, which could be used for drug delivery in the treatment of periodontitis, justified assessing excimer laser surface treatment to promote controlled roughning of hydroxyapatite (HA) and glass-reinforced hydroxyapatite (GR-HA). A KrF excimer laser with 248nm radiation wavelength and 30ns pulse duration was used for surface modification. The laser treatment was carried out in air, using wide ranges of radiation fluence and number of laser pulses. In order to identify the physico-chemical changes induced by the laser treatment and the column formation mechanisms in these materials, the treated surfaces were characterised by laser profilometry, scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infra-red spectroscopy (FTIR). Laser processing induced the formation of a surface topography consisting of cone-shaped features. The constitution of the surface layer was also modified, as revealed by FTIR, XPS and XRD. This work has shown that laser surface modification increases the surface area of HA and GR-HA and is a promising technique to increase the reactivity and drug delivery capability of both materials. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {article, Biocompatible Materials, Biomaterials, drug delivery system, Durapatite, Excimer laser, Excimer lasers, Fourier transform infrared spectroscopy, FTIR, glass, hydroxyapatite, infrared spectroscopy, laser, Lasers, Macromolecular Substances, materials testing, Molecular Conformation, Non-porous bioactive materials, periodontitis, physical chemistry, porosity, priority journal, Profilometry, Radiation Dosage, Reinforcement, Scanning electron microscopy, Surface area, Surface modification, Surface Properties, Surface treatment, topography, X ray diffraction, X ray diffraction analysis, X ray photoelectron spectroscopy, XPS, XRD},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2342427815\&partnerID=40\&md5=24da3c08c0c7b636d9d5039812944b52},
	author = {Queiroz, A.C. and Santos, J.D. and Vilar, R. and Eug{\'e}nio, S. and Monteiro, F.J.}
}
@book {2477,
	title = {Production of porous hydroxyapatite with potential for controlled drug delivery},
	series = {Materials Science ForumMater Sci Forum},
	volume = {455-456},
	year = {2004},
	note = {Conference code: 63332Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: MSFOELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB, Inst. de Engenharia Biom{\'e}dica, R do Campo Alegre, 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., (1987) J. Periodontology, 58, p. 521;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, , Oxford University Press; 
(1996) Journal of Periodontology, 67, p. 831; 
Soskolne, W.A., Heasman, H.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., (1997) Journal of Periodontology, 68, p. 32; 
Da Silva, M.H.P., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., (2002) J. of Non-crystaline Solids, 304, p. 286; 
Chang, B., Lee, C., Hong, K., Youn, H., Ryu, H., Chung, S., Park, K., (2000) Biomat., 21, p. 1291; 
Ewers, R., Simons, B., (1992) Biomaterials - Hard Tissue Repair and Replacement, , Elsevier Sc; 
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Barinov, S.M., Baschenko, Y.V., (1992) Bioceramics and the Human Body, , Elsevier Sc; 
Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., (1997) J. Mater. Sc.: Materials in Medicine, 8, p. 731; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2003) Bioceramics, , accepted for; 
Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterial, 22, p. 1393
},
	month = {2004///},
	pages = {358 - 360},
	address = {Caparica},
	abstract = {Porous hydroxyapatite was prepared in order to be used as a drug carrier and promote bone ingrowth. For this purpose, the microstructure should have interconnective pores and micro as well as macro porosity, for bone cell adhesion and bone ingrowth. The porous hydroxyapatite prepared by the burn-out method exhibits all these features. For measuring porosity, SEM analysis, mercury intrusion porosimetry and BET were used. FT-IR and XRD analysis revealed that only hydroxyapatite phase was present, being this result similar to the one found for dense samples. The morphology obtained appears to be well adapted to bone growth and drug adsorption.},
	keywords = {Adsorption, Antibiotics, Bioceramics, Controlled drug delivery, Drug Carriers, Drug delivery, Fourier transform infrared spectroscopy, hydroxyapatite, morphology, Polyurethanes, porosity, Porous, Porous materials, Scanning electron microscopy, X ray diffraction analysis},
	isbn = {02555476 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17044442244\&partnerID=40\&md5=7baa6f22534360d57deb7862546fb275},
	author = {Queiroz, A.C. and Teixeira, S. and Santos, J.D. and Monteiro, F.J.},
	editor = {Martins R. and Fortunato E. and Ferreira I. and Dias C.}
}
@book {2476,
	title = {Protein adsorption effect on in vitro acellular biodegradation of CaO-P                    2O
                    5 glass ceramics
},
	series = {Materials Science ForumMater Sci Forum},
	volume = {455-456},
	year = {2004},
	note = {Conference code: 63332Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: MSFOELanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptReferences: Zeng, Chittur, K.K., Lacefield, W.R., (1999) Biomaterials, 20, p. 377;Ohta, Monna, H., Takahashi, S., (2001) J. Biomed. Mater. Res., 55, p. 409; 
Zyman, Z., Dedukh, N.V., Glusko, V.I., Shcherbyna, S.V., (2002) 17 th European Society for Biomaterials Conference Proceedings, , Barcelona; 
Krajewski, Malavoti, R., Piancatelli, A., (1996) Biomaterials, 17, p. 53; 
El-Ghannam, Ducheyne, P., Shapiro, I.M., (1997) Biomaterials, 18 (4); 
Takami, (1998) J. Biomed. Mater. Res., 40, p. 24; 
Puleo, A., Bizios, R., (1992) J. Biomed. Mater. Res., 26, p. 291; 
Dubois, C., Souchier, C., Couble, M.L., Exbrayat, P., Lissac, M., (1999) Biomat., 20, p. 1841; 
Green, J., Davies, M.C., Roberts, C.J., Tendler, S.J.B., (1997) Biomaterials, 18, p. 405; 
Haynes, C.A., Norde, W., (1994) Colloids and Surfaces B: Biointerfaces, 2, p. 517; 
Dias, G., Lopes, M.A., Gibson, I.R., Santos, J.D., (2002) 17 th Europ. Soc. for Biomat. Conf. Proc., , Barcelona; 
Chun, Na, S.W., Lee, J.H., Chung, J.P., Ryu, I.C., Kim, S.Y., (2002) Key Eng. Mat., 218-220, p. 149
},
	month = {2004///},
	pages = {398 - 401},
	address = {Caparica},
	abstract = {This work reports the in vitro accelular behaviour of a glass ceramic (45P                        2O
                        5-45CaO-5MgO-5K
                        2O), which has significant potential for being used as an alloplastic bone graft. Experiments were carried out by immersing granule samples for a period up to 90 days in Trisbuffer solution at pH 7.4, in the presence and absence of a foetal bovine serum (FBS). The release of calcium, phosphorus, and potassium ions as well as the amount of protein adsorbed was followed by several techniques. The results obtained seem to indicate that the adsorption of proteins on the surface of the material, although causing an initial delay in the degradation, does not hamper the ionic exchange and therefore it is not a barrier for the degradation of the glass ceramic.
},
	keywords = {Adsorption, Bioceramics, Biodegradation, Calcium Compounds, Foetal bovine serum (FBS), Glass ceramics, Hard tissue, Ion exchange, Ionic strength, pH effects, Protein adhesion, Proteins, Tissue},
	isbn = {02555476 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3142740181\&partnerID=40\&md5=5452672cf92db3be0d9f232ce3b1a83e},
	author = {Dias, A.G. and Lopes, M.A. and Santos, J.D.},
	editor = {Martins R. and Fortunato E. and Ferreira I. and Dias C.}
}
@article {2563,
	title = {Classification of breast tissue by electrical impedance spectroscopy},
	journal = {Medical and Biological Engineering and ComputingMed. Biol. Eng. Comput.},
	volume = {38},
	year = {2000},
	note = {Cited By (since 1996): 42Export Date: 21 November 2012Source: ScopusCODEN: MBECDPubMed ID: 10829386Language of Original Document: EnglishCorrespondence Address: Marques de Sa, J.P.; INEB, Facul. Engenharia Universidade Porto, Porto, Portugal; email: jmsa@fe.op.ptReferences: Brown, B.H., Barber, D.C., Morice, A.H., Leathard, A.D., Cardiac and respiratory related electrical impedance changes in the human thorax (1994) IEEE Trans. Biomed. Eng., BME-41, pp. 729-734;Campbell, A.M., Land, D.V., Dielectric properties of female breast tissue measured in vitro at 3.2 GHz (1992) Phys. Med. Biol., 37, pp. 193-210; 
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Gersing, E., Sch{\"a}fer, M., Osypka, M., The appearance of positive phase angles in impedance measurements on extended biological subjects (1995) Innov. Techn. Biol. Med., 16 (SPEC. ISSUE 2), pp. 72-76. , Proc. {\textquoteright}CAIT Workshop on Electrode-Skin Interface in EIT{\textquoteright}; 
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Jossinet, J., Lavandier, B., The discrimination of excised cancerous breast tissue samples using impedance spectroscopy (1998) Bioelectrochem. Bioenerg., 45, pp. 161-167; 
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Swain, P.H., The decision tree classifier: Design and potential (1977) IEEE Trans. Geosci. Electronics, GE-15, pp. 142-147; 
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Surowiec, A.J., Stuchly, S.S., Barr, J.R., Swarup, A., Dielectric properties of breast carcinoma and the surrounding tissues (1988) IEEE Trans. Biomed. Eng., BME-35, pp. 257-263; 
Tachibana, S., Aguilar, J.A., Birzis, L., Scanning the interior of living brain by impedography (1970) J. of App. Physiol., 28, pp. 534-539
},
	month = {2000///},
	pages = {26 - 30},
	publisher = {Peter Peregrinus Ltd},
	abstract = {Electrical impedance spectroscopy is a minimally invasive technique that has clear advantages for living tissue characterisation owing to its low cost and ease of use. The present paper describes how this technique can be applied to breast tissue classification and breast cancer detection. Statistical analysis is used to derive a set of rules based on features extracted from the graphical representation of electrical impedance spectra. These rules are used hierarchically to discriminate several classes of breast tissue. Results of statistical classification obtained from a data set of 106 cases representing six classes of excised breast tissue show an overall classification efficiency of ~92\% with carcinoma discrimination > 86\%.Electrical impedance spectroscopy is a minimally invasive technique that has clear advantages for living tissue characterization owing to its low cost and ease of use. The present paper describes how this technique can be applied to breast tissue classification and breast cancer detection. Statistical analysis is used to derive a set of rules based on features extracted from the graphical representation of electrical impedance spectra. These rules are used hierarchically to discriminate several classes of breast tissue. Results of statistical classification obtained from a data set of 106 cases representing six classes of excised breast tissue show an overall classification efficiency of approximately 92\% with carcinoma discrimination >86\%.
},
	keywords = {article, Breast, breast cancer, Breast Neoplasms, cancer classification, Data classification, Discriminant Analysis, Electric Impedance, Electrical impedance spectroscopy, Electrodiagnosis, Feature extraction, Female, human, Humans, impedance, Impedance spectroscopy, major clinical study, Medical imaging, Signal Processing, Computer-Assisted, Spectroscopy, Statistical analysis, Statistical methods, Tissue, Tissue characterisation},
	isbn = {01400118 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033962139\&partnerID=40\&md5=1510d493688d338d985aad2a31d1caeb},
	author = {Estrela Da Silva, J. and Marques De S{\'a}, J.P. and Jossinet, J.}
}
@conference {2555,
	title = {A computational model of visual cortex receptive fields using spatio-temporal filters},
	booktitle = {Proceedings of the IEEE/EMBS Region 8 International Conference on Information Technology Applications in Biomedicine, ITABProc IEEE EMBS Reg 8 Int Conf Inf Technol Appl Biomed ITAB},
	series = {ITAB-ITIS 2000},
	year = {2000},
	note = {Conference code: 58015Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Milanova, M.G.; INEB, Inst. of Biomedical Engineering, University of Porto, Pra{\c c}a Coronel Pacheco, 1 4050 Porto, Portugal; email: milanova@ineb.fe.up.ptReferences: Hubel, D.H., Wiesel, T.N., Receptive fields, binocular interaction, and functional architecture in the cat{\textquoteright}s visual cortex (1962) Journal of Physiology (London), 160, pp. 106-154;DeAngelis, G.C., Ohzawa, I., Freeman, R.D., Receptive-field dynamic in the central visual pathways (1995) Trends in Neuroscience, 18, pp. 451-458; 
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Sponsors: IEEE},
	month = {2000///},
	pages = {129 - 134},
	address = {Arlington, VA},
	abstract = {It is known that neurons in the primary visual cortex are characterized by receptive fields that are selective for specific spatial orientations. It is believed that the sense of object motion and velocity are also related to these fields, as objects in natural scenes are represented by a sparse set of statistically independent components, such as edges. Currently, computational models of receptive fields consider only spatial components, and thus cannot account for time-varying sensory stimuli. In this paper, a model based on independent components analysis and cellular neural networks is proposed. This approach extends existing models to incorporate temporal aspects of sequences of images of natural scenes.},
	keywords = {Cellular neural networks, Computational methods, ICA, Independent component analysis, Mathematical models, Neurology, Receptive fields, Receptive fields (RF), Spatial filters, Spatio-temporal filters, Visual modeling},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034451070\&partnerID=40\&md5=f1377b4b2f2403be91a21d0feb639fb7},
	author = {Milanova, M.G. and Elmaghraby, A.S. and Wachowiak, M.P. and Campilho, A.}
}
