



@article {3385,
	title = {Combined C4d and CD3 immunostaining predicts immunoglobulin (Ig)A nephropathy progression},
	journal = {Clinical and Experimental Immunology},
	volume = {179},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {354-361},
	isbn = {0009-9104},
	url = {<Go to ISI>://WOS:000347233800020},
	author = {Faria, B. and Henriques, C. and Matos, A. C. and Daha, M. R. and Pestana, M. and Seelen, M.}
}
@article {3388,
	title = {The interactions between municipal socioeconomic status and age on hip fracture risk},
	journal = {Osteoporosis International},
	volume = {26},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {489-498},
	isbn = {0937-941X},
	url = {<Go to ISI>://WOS:000349018700008},
	author = {Oliveira, C.M. and Economou, T. and Bailey, T. and Mendona, D. and Pina, M.F.}
}
@article {3384,
	title = {Optic disc segmentation using the sliding band filter},
	journal = {Computers in Biology and Medicine},
	volume = {56},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {1-12},
	isbn = {0010-4825},
	url = {<Go to ISI>://WOS:000348265400001},
	author = {Dashtbozorg, Behdad and Mendonca, Ana Maria and Campilho, Aurelio}
}
@article {3393,
	title = {Study of paraffin-embedded colon cancer tissue using terahertz spectroscopy},
	journal = {Journal of Molecular Structure},
	volume = {1079},
	year = {2015},
	note = {Times Cited: 0Carneiro, Fatima/J-6432-2013
Carneiro, Fatima/0000-0002-1964-1006
},
	month = {2015},
	pages = {448-453},
	isbn = {0022-2860},
	url = {<Go to ISI>://WOS:000344444100056},
	author = {Wahaia, Faustino and Kasalynas, Irmantas and Seliuta, Dalius and Molis, Gediminas and Urbanowicz, Andrzej and Carvalho Silva, Catia D. and Carneiro, Fatima and Valusis, Gintaras and Granja, Pedro L.}
}
@article {3394,
	title = {Terahertz spectroscopy for the study of paraffin-embedded gastric cancer samples},
	journal = {Journal of Molecular Structure},
	volume = {1079},
	year = {2015},
	note = {Times Cited: 0Carneiro, Fatima/J-6432-2013
Carneiro, Fatima/0000-0002-1964-1006
},
	month = {2015},
	pages = {391-395},
	isbn = {0022-2860},
	url = {<Go to ISI>://WOS:000344444100049},
	author = {Wahaia, Faustino and Kasalynas, Irmantas and Seliuta, Dalius and Molis, Gediminas and Urbanowicz, Andrzej and Silva, Catia D. Carvalho and Carneiro, Fatima and Valusis, Gintaras and Granja, Pedro L.}
}
@article {3299,
	title = {Actively targeted cetuximab conjugated γ-poly(glutamic acid)-docetaxel nanomedicines for epidermal growth factor receptor over expressing colon cancer cells},
	journal = {Journal of Biomedical Nanotechnology},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {1416 - 1428},
	abstract = {Receptor targeted therapy is advantageous in overcoming the toxicity burden of conventional cancer chemotherapeutics. Over expression of epidermal growth factor receptor (EGFR) on cancer cells and its role in metastasis, malignancy and drug resistance in many human cancers lead to its selection as a promising target for cancer treatment. The present work investigated the preparation and characterization of docetaxel (DTXL) loaded γ-poly (glutamic acid) (γ-PGA) nanoparticles (Nps) conjugated with EGFR antibody (Cetuximab, CET) targeted to colon cancer cells (HT-29), highly over expressing EGFR. The flow cytometric analysis revealed two fold increased cellular uptake of CET-DTXL-γ-PGA Nps by HT-29 (EGFR +ve) cells compared to that of IEC-6 (EGFR-ve) cells confirming the active targeting. Cytotoxicity assays (MTT and LDH) showed superior anti-proliferative activity of CET-DTXL-γ-PGA NPs over DTXL-γ-PGA Nps against HT-29 cells. The cell cycle analysis indicated that CET-DTXL-γ-PGA NPs induced cell death in enhanced percentage of HT-29 cells by undergoing cell cycle arrest in G2/M phase compared to that of DTXL-γ-PGA Nps. The mechanism of cancer cell death was analyzed via apoptotic and mitochondrial membrane potential assays and showed that targeted Nps treatment reduced the mitochondrial membrane potential thereby inducing enhanced HT-29 cell death (apoptosis and necrosis). The biodistribution of targeted and non-targeted Nps were analyzed in vivo in Swiss albino mice using NIR imaging. ICG-CET-DTXL-γ-PGA Nps (targeted) and ICG-DTXL-γ-PGA Nps (non-targeted) followed the similar biodistribution pattern in vivo, but with different elimination time. In short, CET-DTXL-γ-PGA nanoparticles enhance the tumor selective therapeutic efficacy for colon cancer. Copyright {\textcopyright} 2014 American Scientific Publishers All rights reserved.},
	keywords = {Cetuximab, colon cancer, Docetaxel, Epidermal growth factor receptor, Poly(γ-glutamic acid) nanoparticles, Targeted nanomedicines},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84899683740\&partnerID=40\&md5=39d65057952b3e192590262631867971},
	author = {Maya, S. and Sarmento, B. and Lakshmanan, V.-K. and Menon, D. and Jayakumar, R.}
}
@article {3295,
	title = {Adsorbed fibrinogen enhances production of bone- and angiogenic-related factors by monocytes/macrophages},
	journal = {Tissue Engineering - Part A},
	volume = {20},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {250 - 263},
	abstract = {Macrophages are phagocytic cells with great importance in guiding multiple stages of inflammation and tissue repair. By producing a large number of biologically active molecules, they can affect the behavior of other cells and events, such as the foreign body response and angiogenesis. Since protein adsorption to biomaterials is crucial for the inflammatory process, we addressed the ability of the pro-inflammatory molecule fibrinogen (Fg) to modulate macrophage behavior toward tissue repair/regeneration. For this purpose, we used chitosan (Ch) as a substrate for Fg adsorption. Freshly isolated human monocytes were seeded on Ch substrates alone or previously adsorbed with Fg, and allowed to differentiate into macrophages for 10 days. Cell adhesion and morphology, formation of foreign body giant cells (FBGC), and secretion of a total of 80 cytokines and growth factors were evaluated. Both substrates showed similar numbers of adherent macrophages along differentiation as compared with RGD-coated surfaces, which were used as positive controls. Fg did not potentiate FBGC formation. In addition, actin cytoskeleton staining revealed the presence of punctuate F-actin with more elongated and interconnecting cells on Ch substrates. Antibody array screening and quantification of inflammation- and wound-healing-related factors indicated an overall reduction in Ch-based substrates versus RGD-coated surfaces. At late times, most inflammatory agents were down-regulated in the presence of Fg, in contrast to growth factor production, which was stimulated by Fg. Importantly, on Ch+Fg substrates, fully differentiated macrophages produced significant amounts of macrophage inflammatory protein-1delta (MIP-1δ), platelet-derived growth factor-BB, bone morphogenetic protein (BMP)-5, and BMP-7 compared with Ch alone. In addition, other important factors involved in bone homeostasis and wound healing, such as growth hormone, transforming growth factor-β3, and insulin-like growth factor-binding proteins, as well as several angiogenic mediators, including endocrine gland-derived vascular endothelial factor, fibroblast growth factor-7, and placental growth factor, were significantly promoted by Fg. This work provides a new perspective on the inflammatory response in the context of bone repair/regeneration mediated by a pro-inflammatory protein (Fg) adsorbed onto a biomaterial (Ch) that does not otherwise exhibit osteogenic properties. {\textcopyright} Copyright 2014, Mary Ann Liebert, Inc.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84891526984\&partnerID=40\&md5=bf455ae8d2df37fe5c29d41156c8096a},
	author = {Maciel, J. and Oliveira, M.I. and Colton, E. and McNally, A.K. and Oliveira, C. and Anderson, J.M. and Barbosa, M.A.}
}
@article {3237,
	title = {Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand},
	journal = {Journal of the Royal Society, Interface / the Royal Society},
	volume = {11},
	year = {2014},
	month = {2014},
	url = {<Go to ISI>://MEDLINE:25320070},
	author = {Parreira, P. and Shi, Q. and Magalhaes, A. and Reis, C.A. and Bugaytsova, J. and Boren, T. and Leckband, D. and Martins, M.C.L.}
}
@article {3402,
	title = {An Automatic Graph-Based Approach for Artery/Vein Classification in Retinal Images},
	journal = {Ieee Transactions on Image Processing},
	volume = {23},
	year = {2014},
	month = {2014},
	url = {<Go to ISI>://WOS:000335389600001},
	author = {Dashtbozorg, Behdad and Mendonca, Ana Maria and Campilho, Aurelio}
}
@article {3415,
	title = {Bioactivity of surface tethered Osteogenic Growth Peptide motifs},
	journal = {Medchemcomm},
	volume = {5},
	year = {2014},
	note = {Times Cited: 0},
	month = {2014},
	pages = {899-903},
	isbn = {2040-2503},
	url = {<Go to ISI>://WOS:000338431500007},
	author = {Panseri, Silvia and Russo, Laura and Montesi, Monica and Taraballi, Francesca and Cunha, Carla and Marcacci, Maurilio and Cipolla, Laura}
}
@article {3246,
	title = {A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration},
	journal = {Biofabrication},
	volume = {6},
	year = {2014},
	month = {2014},
	url = {<Go to ISI>://WOS:000341823500015},
	author = {Ribeiro, Nilza and Sousa, Susana R. and van Blitterswijk, Clemens A. and Moroni, Lorenzo and Monteiro, Fernando J.}
}
@article {3273,
	title = {Characterization of hLF1-11 immobilization onto chitosan ultrathin films, and its effects on antimicrobial activity},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {3513 - 3521},
	abstract = {hLF1-11 (GRRRRSVQWCA) is an antimicrobial peptide (AMP) with high activity against methicillin-resistant Staphylococcus aureus (MRSA), the most prevalent species in implant-associated infection. In this work, the effect of the surface immobilization on hLF1-11 antimicrobial activity was studied. Immobilization was performed onto chitosan thin films as a model for an implant coating due to its reported osteogenic and antibacterial properties. Chitosan thin films were produced by spin-coating on gold surfaces. hLF1-11 was immobilized onto these films by its C-terminal cysteine in an orientation that exposes the antimicrobial activity-related arginine-rich portion of the peptide. Two levels of exposure (with and without a polyethylene glycol (PEG) spacer) were analyzed. Covalent immobilization was further compared with the AMP physical adsorption onto chitosan films. Surfaces were characterized using ellipsometry, contact angle measurements, atomic force microscopy, infrared and X-ray photoelectron spectroscopies and using a fluorimetric assay for hLF1-11 quantification. Surface antimicrobial activity was assessed through surface adhesion and viability assays using an MRSA (S. aureus ATCC 33591). The incorporation of hLF1-11 increased significantly bacterial adhesion to chitosan films. However, the presence of hLF1-11, namely when immobilized through a PEG spacer, decreased the viability of adherent bacteria with regard to the control surface. These results demonstrated that hLF1-11 after covalent immobilization by its cysteine can maintain activity, particularly if a spacer is applied. However, further studies, exploring the opposite orientation or the same C-terminal orientation, but non-cysteine related, can help to clarify the potential of the hLF1-11 immobilization strategy. {\textcopyright} 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Antimicrobial peptides (AMP), Bacterial adhesion, chitosan, Surface characterization, Surface immobilization},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903724424\&partnerID=40\&md5=e8009cd480b49c37a991753cd145cf34},
	author = {Costa, F. and Maia, S. and Gomes, J. and Gomes, P. and Martins, M.C.L.}
}
@article {3300,
	title = {Chitosan cross-linked docetaxel loaded EGF receptor targeted nanoparticles for lung cancer cells},
	journal = {International Journal of Biological Macromolecules},
	volume = {69},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {532 - 541},
	abstract = {Lung cancer, associated with the up-regulated epidermal growth factor receptor (EGFR) led to the development of EGFR targeted anticancer therapeutics. The biopolymeric nanoparticles form an outstanding system for the targeted delivery of therapeutic agents. The present work evaluated the in vitro effects of chitosan cross-linked γ-poly(glutamic acid) (γ-PGA) nanoparticles (Nps) loaded with docetaxel (DTXL) and decorated with Cetuximab (CET), targeted to EGFR over-expressing non-small-cell-lung-cancer (NSCLC) cells (A549). CET-DTXL-γ-PGA Nps was prepared by ionic gelation and CET conjugation via EDC/NHS chemistry. EGFR specificity of targeted Nps was confirmed by the higher uptake rates of EGFR +ve A549 cells compared to that of EGFR -ve cells (NIH3T3). The cytotoxicity of Nps quantified using cell based (MTT/LDH) and flowcytometry (Cell-cycle analysis, Annexin V/PI and JC-1) assays showed superior antiproliferative activity of CET-DTXL-γ-PGA Nps over DTXL-γ-PGA Nps. The A549 cells treated with CET-DTXL-γ-PGA NPs underwent a G2/M phase cell cycle arrest followed by reduction in mitochondrial membrane potential of A549 cells, inducing apoptosis and necrosis resulting in enhanced cancer cell death. CET-DTXL-γ-PGA Nps exhibited enhanced cellular internalization and therapeutic activity, by actively targeting EGFR on NSCLC cells and hence could be an effective alternative to non-specific, conventional chemotherapy by increasing its efficiency by many folds. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {Cetuximab, chitosan, Epidermal growth factor receptor, Poly(γ-glutamic acid) nanoparticles, Targeted nanoparticles},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903523289\&partnerID=40\&md5=728cbd3502e7922319f5f88fa8d6ec6d},
	author = {Maya, S. and Sarmento, B. and Lakshmanan, V.-K. and Menon, D. and Seabra, V. and Jayakumar, R.}
}
@article {3322,
	title = {Chitosan-modified porous silicon microparticles for enhanced permeability of insulin across intestinal cell monolayers},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {7172 - 7179},
	abstract = {Porous silicon (PSi) based particulate systems are emerging as an important drug delivery system due to its advantageous properties such as biocompatibility, biodegradability and ability to tailor the particles{\textquoteright} physicochemical properties. Here, annealed thermally hydrocarbonized PSi (AnnTHCPSi) and undecylenic acid modified AnnTHCPSi (AnnUnTHCPSi) microparticles were developed as a PSi-based platform for oral delivery of insulin. Chitosan (CS) was used to modify the AnnUnTHCPSi microparticles to enhance the intestinal permeation of insulin. Surface modification with CS led to significant increase in the interaction of PSi microparticles with Caco-2/HT-29 cell co-culture monolayers. Compared to pure insulin, the CS-conjugated microparticles significantly improved the permeation of insulin across the Caco-2/HT-29 cell monolayers, with ca. 20-fold increase in the amount of insulin permeated and ca. 7-fold increase in the apparent permeability (Papp) value. Moreover, among all the investigated particles, the CS-conjugated microparticles also showed the highest amount of insulin associated with the mucus layer and the intestinal Caco-2 cells and mucus secreting HT-29 cells. Our results demonstrate that CS-conjugated AnnUnTHCPSi microparticles can efficiently enhance the insulin absorption across intestinal cells, and thus, they are promising microsystems for the oral delivery of proteins and peptides across the intestinal cell membrane. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {Cell monolayers, chitosan, Diabetes, Insulin, Microparticles, Porous silicon},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84902079022\&partnerID=40\&md5=4de20e5572512006f72e11cb61a2035c},
	author = {Shrestha, N. and Shahbazi, M.-A. and Ara{\'u}jo, F. and Zhang, H. and M{\"a}kil{\"a}, E.M. and Kauppila, J. and Sarmento, B. and Salonen, J.J. and Hirvonen, J.T. and Santos, H.A.}
}
@article {3232,
	title = {Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated PLGA nanoparticles for theranostic application},
	journal = {International Journal of Nanomedicine},
	volume = {9},
	year = {2014},
	month = {2014},
	pages = {4911-4922},
	url = {<Go to ISI>://WOS:000343406300001},
	author = {Moura, Catarina Costa and Segundo, Marcela A. and das Neves, Jose and Reis, Salette and Sarmento, Bruno}
}
@article {3325,
	title = {Collagen surface modified poly(ε-caprolactone) scaffolds with improved hydrophilicity and cell adhesion properties},
	journal = {Materials Letters},
	volume = {134},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {263 - 267},
	abstract = {Poly(ε-caprolactone) (PCL)/collagen scaffolds were produced using a four-step procedure comprising (i) the fabrication of PCL scaffolds through an additive bioextrusion system, (ii) the PCL surface modification using a plasma treatment, (iii) the acrylic acid (AAc) grafting by ultraviolet (UV) polymerization, and (iv) the immobilization of collagen into the scaffold surface through the carbodiimide chemistry. This procedure allowed the quick fabrication of highly porous scaffolds with interconnected pores, well-defined internal architecture and improved cell adhesion properties. The immobilization of collagen into the PCL surface led to a significant enhancement in the hydrophilicity, while the biological tests showed a further improvement in the adhesion of fibroblast cells. These results indicate that the collagen surface modification is an effective strategy to render the hydrophobic nature of PCL and overcome the limited cell interaction of 3D PCL scaffolds. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {BioExtruder, Collagen, poly(ε-caprolactone), Scaffold, Surface modification},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905669651\&partnerID=40\&md5=ebb38981b89fc93e5599a7592a37e134},
	author = {Sousa, I. and Mendes, A. and Pereira, R.F. and B{\'a}rtolo, P.J.}
}
@article {3324,
	title = {DNAJB4 molecular chaperone distinguishes WT from mutant E-cadherin, determining their fate in vitro and in vivo},
	journal = {Human Molecular Genetics},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2094 - 2105},
	abstract = {E-cadherin (Ecad) is a well-known invasion suppressor and its loss of expression is common in invasive carcinomas. Germline Ecad mutations are the only known genetic cause of hereditary diffuse gastric cancer (HDGC), demonstrating the causative role of Ecad impairment in gastric cancer. HDGC-associated Ecad missense mutations can lead to folding defects and premature proteasome-dependent endoplasmic reticulum-associated degradation (ERAD), but the molecular determinants for this fate were unidentified. Using a Drosophilabased genetic screen, we found that Drosophila DnaJ-1 interacts with wild type (WT) and mutant human Ecad in vivo. DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4), the human homolog of DnaJ-1, influences Ecad localization and stability even in the absence of Ecad endogenous promoter, suggesting a post-transcriptional level of regulation. Increased expression of DNAJB4 leads to stabilization of WT Ecad in the plasma membrane, while it induces premature degradation of unfolded HDGC mutants in the proteasome. The interaction between DNAJB4 and Ecad is direct, and is increased in the context of the unfolded mutant E757K, especially when proteasome degradation is inhibited, suggesting that DNAJB4 is a molecular mediator of ERAD. Post-translational regulation of native Ecad by DNAJB4 molecular chaperone is sufficient to influence cell adhesion in vitro. Using a chick embryo chorioallantoic membrane assay with gastric cancer derived cells, we demonstrate that DNAJB4 stimulates the anti-invasive function of WT Ecad in vivo. Additionally, the expression of DNAJB4 and Ecad is concomitantly decreased in human gastric carcinomas. Altogether, we demonstrate that DNAJB4 is a sensor of Ecad structural features that might contribute to gastric cancer progression. {\textcopyright} The Author 2013. Published by Oxford University Press. All rights reserved.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84897831766\&partnerID=40\&md5=c7651a5372b8a46de1f028888590b3f3},
	author = {Sim{\~o}es-Correia, J. and Silva, D.I. and Melo, S. and Figueiredo, J. and Caldeira, J. and Pinto, M.T. and Gir{\~a}o, H. and Pereira, P. and Seruca, R.}
}
@article {3278,
	title = {Dysregulation of T cell receptor N-glycosylation: A molecular mechanism involved in ulcerative colitis},
	journal = {Human Molecular Genetics},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2416 - 2427},
	abstract = {The incidence of inflammatory bowel disease is increasing worldwide and the underlying molecular mechanisms are far from being fully elucidated. Herein, we evaluated the role of N-glycosylation dysregulation in T cells as a key mechanism in the ulcerative colitis (UC) pathogenesis. The evaluation of the branched N-glycosylation levelsandprofile of intestinalTcell receptor (TCR)wereassessedin colonic biopsies fromUCpatientsand healthy controls. Expression alterations of the glycosyltransferase gene MGAT5 were also evaluated. We demonstrated thatUCpatients exhibit a dysregulation ofTCRbranchedN-glycosylationonlamina propriaTlymphocytes. Patients with severe UC showed the most pronounced defect on N-glycan branching in T cells. Moreover, UC patients showed a significant reduction of MGAT5 gene transcription in T lymphocytes. In this study, we disclose for the first time that a deficiency in branched N-glycosylation on TCR due to a reduced MGAT5 gene expression is a new molecular mechanism underlying UC pathogenesis, being a potential novel biomarker with promising clinical and therapeutic applications. {\textcopyright} The Author 2013. Published by Oxford University Press. All rights reserved.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84897565140\&partnerID=40\&md5=4ee6b5b3f85e16eba322ceb8e0d69dd3},
	author = {Dias, A.M. and Dourado, J. and Lago, P. and Cabral, J. and Marcos-Pinto, R. and Salgueiro, P. and Almeida, C.R. and Carvalho, S. and Fonseca, S. and Lima, M. and Vilanova, M. and Dinis-Ribeiro, M. and Reis, C.A. and Pinho, S.S.}
}
@article {3298,
	title = {Effect of cell density on mesenchymal stem cells aggregation in RGD-alginate 3D matrices under osteoinductive conditions},
	journal = {Macromolecular Bioscience},
	volume = {14},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {759 - 771},
	abstract = {Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered "normal" activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell-cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering. {\textcopyright} 2014 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {alginate hydrogels, encapsulation, Self-assembly, Tissue engineering},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84902536382\&partnerID=40\&md5=d6659c40247c561753aca566f938d788},
	author = {Maia, F.R. and Louren{\c c}o, A.H. and Granja, P.L. and Gon{\c c}alves, R.M. and Barrias, C.C.}
}
@article {3294,
	title = {Eradication of Helicobacter pylori: Past, present and future},
	journal = {Journal of Controlled Release},
	volume = {189},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {169 - 186},
	abstract = {Helicobacter pylori is the major cause of chronic gastritis and peptic ulcers. Since the classification as a group 1 carcinogenic by International Agency for Research on Cancer, the importance of the complete H. pylori eradication has obtained a novel meaning. Hence, several studies have been made in order to deepen the knowledge in therapy strategies. However, the current therapy presents unsatisfactory eradication rates due to the lack of therapeutic compliance, antibiotic resistance, the degradation of antibiotics at gastric pH and their insufficient residence time in the stomach. Novel approaches have been made in order to overcome these limitations. The purpose of this review is to provide an overview about the current therapy and its limitations, while highlighting the possibility of using micro- and nanotechnology to develop gastric drug delivery systems, overcoming these difficulties in the future. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {Drug delivery, Helicobacter pylori, Microparticles, nanoparticles, Treatment},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904904149\&partnerID=40\&md5=96cfe2d7f96a7c7a16bb7eb733c2b71c},
	author = {Lopes, D. and Nunes, C. and Martins, M.C.L. and Sarmento, B. and Reis, S.}
}
@article {3399,
	title = {Extracellular matrix stiffness and composition jointly regulate the induction of malignant phenotypes in mammary epithelium},
	journal = {Nature Materials},
	volume = {13},
	year = {2014},
	note = {Times Cited: 6},
	month = {2014},
	pages = {970-978},
	isbn = {1476-1122},
	url = {<Go to ISI>://WOS:000342743100018},
	author = {Chaudhuri, Ovijit and Koshy, Sandeep T. and Branco da Cunha, Cristiana and Shin, Jae-Won and Verbeke, Catia S. and Allison, Kimberly H. and Mooney, David J.}
}
@article {3419,
	title = {Fluorescent bionanoprobes based on quantum dot-chitosan-O-phospho-L-serine conjugates for labeling human bone marrow stromal cells},
	journal = {Rsc Advances},
	volume = {4},
	year = {2014},
	month = {2014},
	pages = {49016-49027},
	url = {<Go to ISI>://WOS:000343711000035},
	author = {Salgado, Christiane L. and Mansur, Alexandra A. P. and Mansur, Herman S. and Monteiro, Fernando J. M.}
}
@article {3249,
	title = {HA/TCP Scaffolds Obtained by Sucrose Crystal Leaching Method: Preliminary in vitro Evaluation},
	journal = {Materials Research-Ibero-American Journal of Materials},
	volume = {17},
	year = {2014},
	month = {2014},
	pages = {811-816},
	url = {<Go to ISI>://WOS:000342348700003},
	author = {Rodrigues, Leonardo Ribeiro and Laranjeira, Marta de Sousa and Fernandes, Maria Helena and Monteiro, Fernando Jorge and de Carvalho Zavaglia, Cecilia Amelia}
}
@article {3303,
	title = {Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure},
	journal = {ACS Nano},
	volume = {8},
	year = {2014},
	note = {Cited By (since 1996):4Export Date: 21 November 2014},
	month = {2014},
	pages = {2033 - 2047},
	abstract = {Stem cell responsiveness to extracellular matrix (ECM) composition and mechanical cues has been the subject of a number of investigations so far, yet the molecular mechanisms underlying stem cell mechano-biology still need full clarification. Here we demonstrate that the paralog proteins YAP and TAZ exert a crucial role in adult cardiac progenitor cell mechano-sensing and fate decision. Cardiac progenitors respond to dynamic modifications in substrate rigidity and nanopattern by promptly changing YAP/TAZ intracellular localization. We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design. {\textcopyright} 2014 American Chemical Society.},
	keywords = {adult cardiac progenitor cell, cardiac differentiation, mechano- transduction, substrate nanotopography, YAP/TAZ},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896911335\&partnerID=40\&md5=c15ea687a167227f6d91e71dcc812590},
	author = {Mosqueira, D. and Pagliari, S. and Uto, K and Ebara, M and Romanazzo, S. and Escobedo-Lucea, C and Nakanishi, J and Taniguchi, A and Franzese, O and Di Nardo, P. and Goumans, MJ and Traversa, E and Pinto-Do-{\'O}, P. and Aoyagi, T. and Forte, G.}
}
@article {3305,
	title = {Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms},
	journal = {Stem Cell Research and Therapy},
	volume = {5},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	abstract = {Introduction. Among the plethora of cells under investigation to restore a functional myocardium, mesenchymal stromal cells (MSCs) have been granted considerable interest. However, whereas the beneficial effects of bone marrow MSCs (BM-MSCs) in the context of the diseased heart are widely reported, data are still scarce on MSCs from the umbilical cord matrix (UCM-MSCs). Herein we report on the effect of UCM-MSC transplantation to the infarcted murine heart, seconded by the dissection of the molecular mechanisms at play. Methods. Human umbilical cord tissue-derived MSCs (UCX{\textregistered}), obtained by using a proprietary technology developed by ECBio, were delivered via intramyocardial injection to C57BL/6 females subjected to permanent ligation of the left descending coronary artery. Moreover, medium produced by cultured UCX{\textregistered} preconditioned under normoxia (CM) or hypoxia (CMH) was collected for subsequent in vitro assays. Results: Evaluation of the effects upon intramyocardial transplantation shows that UCX{\textregistered} preserved cardiac function and attenuated cardiac remodeling subsequent to myocardial infarction (MI). UCX{\textregistered} further led to increased capillary density and decreased apoptosis in the injured tissue. In vitro, UCX{\textregistered}-conditioned medium displayed (a) proangiogenic activity by promoting the formation of capillary-like structures by human umbilical vein endothelial cells (HUVECs), and (b) antiapoptotic activity in HL-1 cardiomyocytes subjected to hypoxia. Moreover, in adult murine cardiac Sca- 1{\textsection}ssup{\textsection}+{\textsection}esup{\textsection} progenitor cells (CPCs), conditioned medium enhanced mitogenic activity while activating a gene program characteristic of cardiomyogenic differentiation. Conclusions: UCX{\textregistered} preserve cardiac function after intramyocardial transplantation in a MI murine model. The cardioprotective effects of UCX{\textregistered} were attributed to paracrine mechanisms that appear to enhance angiogenesis, limit the extent of the apoptosis, augment proliferation, and activate a pool of resident CPCs. Overall, these results suggest that UCX{\textregistered} should be considered an alternative cell source when designing new therapeutic approaches to treat MI. {\textcopyright} 2014 Nascimento et al.; licensee BioMed Central Ltd.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896713037\&partnerID=40\&md5=22fdca81d834ec78c8196552c3d250ae},
	author = {Nascimento, D.S. and Mosqueira, D. and Sousa, L.M. and Teixeira, M. and Filipe, M. and Resende, TP and Ara{\'u}jo, A.F. and Valente, M. and Almeida, J. and Martins, J.P. and Santos, J.M. and B{\'a}rcia, R.N. and Cruz, P. and Cruz, H. and Pinto-Do-{\'O}, P.}
}
@article {3296,
	title = {Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells},
	journal = {Journal of Controlled Release},
	volume = {189},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {158 - 168},
	abstract = {The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {Bone Regeneration, Co-delivery systems, Guided differentiation, Injectable alginate hydrogels, Mesenchymal Stem Cells, Osteoinductive peptides},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904318888\&partnerID=40\&md5=5f671d3584c3b7fc785ffaf40c2f0b1d},
	author = {Maia, F.R. and Barbosa, M. and Gomes, D.B. and Vale, N. and Gomes, P. and Granja, P.L. and Barrias, C.C.}
}
@article {3264,
	title = {The impact of nanoparticles on the mucosal translocation and transport of GLP-1 across the intestinal epithelium},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {9199 - 9207},
	abstract = {Glucagon like peptide-1 (GLP-1) is an incretin hormone that is in the pipeline for type 2 diabetes mellitus (T2DM) therapy. However, oral administration of GLP-1 is hindered by the harsh conditions of the gastrointestinal tract and poor bioavailability. In this study, three nanosystems composed by three different biomaterials (poly(lactide-co-glycolide) polymer (PLGA), Witepsol E85 lipid (solid lipid nanoparticles, SLN) and porous silicon (PSi) were developed and loaded with GLP-1 to study their permeability invitro. All the nanoparticles presented a size of approximately 200nm. The nanoparticles{\textquoteright} interaction with the mucus and the intestinal cells were enhanced after coating with chitosan (CS). PSi nanosystems presented the best association efficiency (AE) and loading degree (LD), even though a high AE was also observed for PLGA nanoparticles and SLN. Among all the nanosystems, PLGA and PSi were the only nanoparticles able to sustain the release of GLP-1 in biological fluids when coated with CS. This characteristic was also maintained when the nanosystems were in contact with the intestinal Caco-2 and HT29-MTX cell monolayers. The CS-coated PSi nanoparticles showed the highest GLP-1 permeation across the intestinal invitro models. In conclusion, PLGA+CS and PSi+CS are promising nanocarriers for the oral delivery of GLP-1. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {chitosan, Diabetes, Glucagon like peptide-1, nanoparticles, Oral delivery systems, Triple co-culture},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84906788144\&partnerID=40\&md5=38c557a51eb271561ef59bfe12421842},
	author = {Ara{\'u}jo, F. and Shrestha, N. and Shahbazi, M.-A. and Fonte, P. and M{\"a}kil{\"a}, E.M. and Salonen, J.J. and Hirvonen, J.T. and Granja, P.L. and Santos, H.A. and Sarmento, B.}
}
@article {3265,
	title = {Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation},
	journal = {Journal of Biomaterials Applications},
	volume = {28},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1325 - 1335},
	abstract = {Nanohydroxyapatite (nanoHA), due to its chemical properties, has appeared as an exceptionally promising bioceramic to be used as bone regeneration material. Staphylococcus epidermidis have emerged as major nosocomial pathogens associated with infections of implanted medical devices. In this work, the purpose was to study the influence of the nanoHA surface characteristics on S. epidermidis RP62A biofilm formation. Therefore, two different initial inoculum concentrations (Ci) were used in order to check if these would affect the biofilm formed on the nanoHA surfaces. Biofilm formation was followed by the enumeration of cultivable cells and by scanning electron microscopy. Surface topography, contact angle, total surface area and porosimetry of the biomaterials were studied and correlated with the biofilm data. The surface of nanoHA sintered at 830 (nanoHA830) showed to be more resistant to S. epidermidis attachment and accumulation than that of nanoHA sintered at 1000 (nanoHA1000). The biofilm formed on nanoHA830 presented differences in terms of structure, surface coverage and EPS production when compared to the one formed on nanoHA1000 surface. It was observed that topography and surface area of nanoHA surfaces had influence on the bacterial attachment and accumulation. Ci influenced bacteria attachment and accumulation on nanoHA surfaces over time. The choice of the initial inoculum concentration was relevant proving to have an effect on the extent of adherence thus being a critical point for human health if these materials are used in implantable devices. This study showed that the initial inoculum concentration and surface material properties determine the rate of microbial attachment to substrata and consequently are related to biofilm-associated infections in biomaterials. {\textcopyright} The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.},
	keywords = {biofilm formation, biomaterial roughness, biomaterial surface area, Inoculum concentration, Nanohydroxyapatite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84890640286\&partnerID=40\&md5=0f7d5eb37db34b3d0a1e4fb334846340},
	author = {Barros, J. and Grenho, L. and Manuel, C.M. and Ferreira, C. and Melo, L. and Nunes, O.C. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {3268,
	title = {Influence of the stiffness of three-dimensional alginate/collagen-I interpenetrating networks on fibroblast biology},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {8927 - 8936},
	abstract = {Wound dressing biomaterials are increasingly being designed to incorporate bioactive molecules to promote healing, but the impact of matrix mechanical properties on the biology of resident cells orchestrating skin repair and regeneration remains to be fully understood. This study investigated whether tuning the stiffness of a model wound dressing biomaterial could control the behavior of dermal fibroblasts. Fully interpenetrating networks (IPNs) of collagen-I and alginate were fabricated to enable gel stiffness to be tuned independently of gel architecture, polymer concentration or adhesion ligand density. Three-dimensional cultures of dermal fibroblasts encapsulated within matrices of different stiffness were shown to promote dramatically different cell morphologies, and enhanced stiffness resulted in upregulation of key-mediators of inflammation such as IL-10 and COX-2. These findings suggest that simply modulating the matrix mechanical properties of a given wound dressing biomaterial deposited at the wound site could regulate the progression of wound healing. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {ECM (extracellular matrix), Inflammation, Interpenetrating networks (IPNs), Mechanical properties, Wound dressing biomaterial, Wound healing},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905723265\&partnerID=40\&md5=dc192cf150d9176075beca26ae5ded3f},
	author = {Branco da Cunha, C. and Klumpers, D.D. and Li, W.A. and Koshy, S.T. and Weaver, J.C. and Chaudhuri, O. and Granja, P.L. and Mooney, D.J.}
}
@article {3280,
	title = {Injectable MMP-sensitive alginate hydrogels as hMSC delivery systems},
	journal = {Biomacromolecules},
	volume = {15},
	year = {2014},
	note = {Cited By (since 1996):3Export Date: 21 November 2014},
	month = {2014},
	pages = {380 - 390},
	abstract = {Hydrogels with the potential to provide minimally invasive cell delivery represent a powerful tool for tissue-regeneration therapies. In this context, entrapped cells should be able to escape the matrix becoming more available to actively participate in the healing process. Here, we analyzed the performance of proteolytically degradable alginate hydrogels as vehicles for human mesenchymal stem cells (hMSC) transplantation. Alginate was modified with the matrix metalloproteinase (MMP)-sensitive peptide Pro-Val-Gly-Leu-Iso-Gly (PVGLIG), which did not promote dendritic cell maturation in vitro, neither free nor conjugated to alginate chains, indicating low immunogenicity. hMSC were entrapped within MMP-sensitive and MMP-insensitive alginate hydrogels, both containing cell-adhesion RGD peptides. Softer (2 wt \% alginate) and stiffer (4 wt \% alginate) matrices were tested. When embedded in a Matrigel layer, hMSC-laden MMP-sensitive alginate hydrogels promoted more extensive outward cell migration and invasion into the tissue mimic. In vivo, after 4 weeks of subcutaneous implantation in a xenograft mouse model, hMSC-laden MMP-sensitive alginate hydrogels showed higher degradation and host tissue invasion than their MMP-insensitive equivalents. In both cases, softer matrices degraded faster than stiffer ones. The transplanted hMSC were able to produce their own collagenous extracellular matrix, and were located not only inside the hydrogels, but also outside, integrated in the host tissue. In summary, injectable MMP-sensitive alginate hydrogels can act as localized depots of cells and confer protection to transplanted cells while facilitating tissue regeneration. {\textcopyright} 2013 American Chemical Society.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84892606344\&partnerID=40\&md5=835c11a6460fa62bc6da4a189fd06b25},
	author = {Fonseca, K.B. and Gomes, D.B. and Lee, K. and Santos, S.G. and Sousa, A. and Silva, E.A. and Mooney, D.J. and Granja, P.L. and Barrias, C.C.}
}
@article {3302,
	title = {Injectable pectin hydrogels produced by internal gelation: PH dependence of gelling and rheological properties},
	journal = {Carbohydrate Polymers},
	volume = {103},
	year = {2014},
	note = {Cited By (since 1996):5Export Date: 21 November 2014},
	month = {2014},
	pages = {339 - 347},
	abstract = {The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery. {\textcopyright} 2013 Elsevier Ltd.},
	keywords = {Biomaterials, Internal gelation, Natural polymers, pectin, Tissue engineering},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84892575017\&partnerID=40\&md5=91c88ab2cf99ad8c279d1253e930ef01},
	author = {Moreira, H.R. and Munarin, F. and Gentilini, R. and Visai, L. and Granja, P.L. and Tanzi, M.C. and Petrini, P.}
}
@article {3397,
	title = {Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces},
	journal = {Nature Cell Biology},
	volume = {16},
	year = {2014},
	note = {Times Cited: 0},
	month = {2014},
	pages = {1249-U259},
	isbn = {1465-7392},
	url = {<Go to ISI>://WOS:000345777300015},
	author = {Barisic, Marin and Aguiar, Paulo and Geley, Stephan and Maiato, Helder}
}
@article {3262,
	title = {Long-term effects of chronic cocaine exposure throughout adolescence on anxiety and stress responsivity in a Wistar rat model},
	journal = {Neuroscience},
	volume = {277},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {343 - 355},
	abstract = {Adolescents display increased vulnerability to engage in drug experimentation. This is often considered a risk factor for later drug abuse. In this scenario, the permanent effects of cocaine exposure during adolescence on anxiety levels and stress responsivity, which may result in behavioral phenotypes prone to addiction, are now starting to be unveiled. Thus, the purpose of the present study was to evaluate the long-lasting effects of chronic cocaine administration during adolescence, on anxiety-like behavior and on stress response. Adolescent male Wistar rats were daily administered 45-mg cocaine/kg of body weight in three equal intraperitoneal doses with 1-h interval, from postnatal day (PND) 35 to 50. The effects of cocaine administration on anxiety levels, assessed in the Elevated Plus Maze (EPM), and on social stress response, assessed in the resident-intruder paradigm (R/I), were evaluated 10. days after withdrawal, when rats were reaching the adulthood. The underlying dopaminergic activity, and the corticosterone and testosterone levels were determined. Our results showed that cocaine induced long-lasting alterations in the hypothalamus-pituitary-adrenals (HPA) axis function and in testosterone levels. Such alterations resulted in significant and enduring changes in behavioral responses to environmental challenges, such as the EPM and R/I, including the evaluation of potential threats that may lead to high-risk behavior and low-benefit choices. This was further supported by an altered dopaminergic function in the amygdala and hippocampus. The present findings provide new insights into how the use of cocaine during adolescent development may modulate emotional behavior later in life. Compromised ability to recognize and deal with potential threats is an important risk factor to perpetuate compulsive drug seeking and relapse susceptibility. {\textcopyright} 2014 IBRO.},
	keywords = {Adolescence, anxiety, Cocaine, corticosterone, Dopaminergic system, Social stress},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905366454\&partnerID=40\&md5=d2e5800617d04560c58988a8957e2a5b},
	author = {Alves, C.J. and Magalh{\~a}es, A. and Melo, P. and De Sousa, L. and Tavares, M.A. and Monteiro, P.R.R. and Summavielle, T.}
}
@article {3270,
	title = {Macrophages stimulate gastric and colorectal cancer invasion through EGFR Y 1086, c-Src, Erk1/2 and Akt phosphorylation and smallGTPase activity},
	journal = {Oncogene},
	volume = {33},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {2123 - 2133},
	abstract = {The interactions between cancer cells and their microenvironment are crucial for malignant progression, as they modulate invasion-related activities. Tumor-associated macrophages are generally considered allies in the process of tumor progression in several types of cancer, although their role on gastric and colorectal carcinomas is still poorly understood. In this report, we studied the influence of primary human macrophages on gastric and colorectal cancer cells, considering invasion, motility/migration, proteolysis and activated intracellular signaling pathways. We demonstrated that macrophages stimulate cancer cell invasion, motility and migration, and that these effects depend on matrix metalloproteinase (MMP) activity and on the activation of epidermal growth factor receptor (EGFR) (at the residue Y 1086), PLC-γ (phospholipase C-gamma) and Gab1 (GRB2-associated binding protein-1), as evidenced by siRNA (small interference RNA) experiments. Epidermal growth factor (EGF)-immunodepletion impaired macrophage-mediated cancer cell invasion and motility, suggesting that EGF is the pro-invasive and pro-motile factor produced by macrophages. Macrophages also induced gastric and colorectal cancer cell phosphorylation of Akt, c-Src and ERK1/2, and led to an increase of RhoA and Cdc42 activity. Interestingly, whereas macrophage-mediated cancer cell c-Src and ERK1/2 phosphorylation occurred downstream EGFR activation, Akt phosphorylation seems to be a parallel event, taking place in an EGFR-independent manner. The involvement of EGF, EGFR-downstream signaling partners and MMPs in macrophage-mediated invasion provides novel insights into the molecular crosstalk established between cancer cells and macrophages, opening new perspectives for the design of new and more efficient therapeutic strategies to counteract cancer cell invasion. {\textcopyright} 2014 Macmillan Publishers Limited.},
	keywords = {cancer cell invasion, colon cancer, Gastric cancer, invasion-related signaling pathways, Macrophages, tumor microenvironment},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84899425243\&partnerID=40\&md5=beff5192ff42940c75fef8b48ebd3ae7},
	author = {Cardoso, A.P. and Pinto, M.L. and Pinto, A.T. and Oliveira, M.I. and Pinto, M.T. and Gon{\c c}alves, R. and Relvas, J.B. and Figueiredo, C. and Seruca, R. and Mantovani, A. and Mareel, M. and Barbosa, M.A. and Oliveira, M.J.}
}
@article {3297,
	title = {Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {3197 - 3208},
	abstract = {Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions. The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems. Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated. MSC aggregation occurred only in more compliant matrices (G' <= 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM). Fibronectin assembly stabilized cell-cell contacts within aggregates, even in non-adhesive matrices. However, MSCs were able to substantially contract the artificial matrix only when RGD was present. Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD. Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate. These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices. {\textcopyright} 2014 Published by Elsevier Ltd. on behalf of Acta Materialia Inc.},
	keywords = {cell aggregation, Cell traction, Cell-matrix interactions, Hydrogels, Viscoelastic properties},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84901785690\&partnerID=40\&md5=3d3a11adce17a7f1be2a2bb5d4e51089},
	author = {Maia, F.R. and Fonseca, K.B. and Rodrigues, G. and Granja, P.L. and Barrias, C.C.}
}
@article {3413,
	title = {Microfluidics co-culture systems for studying tooth innervation},
	journal = {Frontiers in Physiology},
	volume = {5},
	year = {2014},
	note = {Times Cited: 0Lamghari, Meriem/K-2159-2013
Lamghari, Meriem/0000-0002-2500-4548
},
	month = {2014},
	isbn = {1664-042X},
	url = {<Go to ISI>://WOS:000347083900001},
	author = {Pagella, Pierfrancesco and Neto, Estrela and Jimenez-Rojo, Lucia and Lamghari, Meriem and Mitsiadis, Thimios A.}
}
@article {3292,
	title = {Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications},
	journal = {Science and Technology of Advanced Materials},
	volume = {15},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol-gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior. {\textcopyright} 2014 National Institute for Materials Science.},
	keywords = {bacteria adhesion, Biomaterials, hemolysis, human dermal microvascular endothelial cells (HDMECs), human gingival fibroblasts (HGFs), Micropatterning, Soft lithography},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84898687729\&partnerID=40\&md5=f252b87aeb163775547158cad0cf658b},
	author = {Laranjeira, M.S. and Carvalho, {\^A}. and Pelaez-Vargas, A. and Hansford, D. and Ferraz, M.P. and Coimbra, S. and Costa, E. and Santos-Silva, A. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {3321,
	title = {Natriuretic peptide system modulation in uninephrectomized rats},
	journal = {Experimental and Clinical Cardiology},
	volume = {20},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2368 - 2387},
	abstract = {BACKGROUND: Cardiac activation of the natriuretic peptide (NP) system plays a major role in the renal adaptations to volume, expansion (VE). However, its role after uninephrectomy (Unx) remains to be elucidated. In the present study, we evaluated cardiac hemodynamics, plasma NP-Type B (BNP) levels nd NP receptor A (NPR-A) and C (NPR-C) expression in the renal cortex (RC) and medulla (RM) of Sham and Unx rats. METHODS: Male Wistar-Han rats (n=42) were randomly assigned to Unx or Sham surgery. Non-invasive blood pressure (BP), invasive left ventricular (LV) hemodynamics and natriuretic response to VE were performed and plasma was collected for BNP determination, 2, 10 and 26 weeks after surgery. In addition, samples from RC and RM were obtained for NPR-A and -C quantification (RT-PCR and western-blot). RESULTS: In Unx, blood pressure elevation and reduced natriuretic response to VE were observed. Although plasma BNP levels increased overtime, no LV dysfunction or myocardial BNP gene activation was observed in Unx. NPR-A mRNA in RC and RM were significantly increased in Unx at 26 weeks. This was accompanied by decreased NPR-C mRNA in the RM in Unx at 10 and 26 weeks, with no differences detected in the RC. CONCLUSIONS: In our experimentalmodel of Unx, progressive BP elevation and reduced natriuretic response to VE were accompanied by modulation of NPR-A and -C expression, in both the RC and RM, in the absence of cardiac activation of the NP system. Our results suggest a local renal modulation of the NP system after Unx. {\textcopyright} 2013 et al.; licensee Cardiology Academic Press.},
	keywords = {BNP, Hypertension, Natriuretic peptide receptor, Uninephrectomy},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905968553\&partnerID=40\&md5=54369af569474ee0bfddfa5aeac3237a},
	author = {Santos-Ara{\'u}jo, C. and Roncon-Albuquerque Jr., R. and Moreira-Rodrigues, M. and Henriques-Coelho, T. and Quelhas-Santos, J. and Faria, B. and Sampaio-Maia, B. and Leite-Moreira, A.F. and Pestana, M.}
}
@article {3269,
	title = {Optimization of the production of solid Witepsol nanoparticles loaded with rosmarinic acid},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {115},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {109 - 117},
	abstract = {During the last decade there has been a growing interest in the formulation of new food and nutraceutical products containing compounds with antioxidant activity. Unfortunately, due to their structure, certain compounds such as polyphenols, in particular rosmarinic acid (RA) are not stable and may interact easily with matrices in which they are incorporated. To overcome such limitations, the formulation of loaded polyphenols nanoparticles can offer an efficient solution to protect such compounds. Based on this rationale, the aim of this study was to prepare solid lipid nanoparticles (SLNs) loaded with RA using a hot melt ultrasonication method, where Witepsol H15 was used as lipid and Polysorbate 80 (Tween 80) as surfactant, following a 32 fractional factorial design, resulting in the use of 3 different percentages of surfactant (viz. 1, 2 and 3\%, v/v) and lipid (0.5, 1.0 and 1.5\%, w/v). The stability of the nanoparticles systems were tested during 28d in aqueous solution stored at refrigeration temperature (ca. 5{\textdegree}C), tracking the mean particle size of different formulations by photon correlation spectroscopy. To confirm RA entrapment, thermal analyses of the nanoparticles by DSC and FTIR were performed. The association efficiencies percentages (AE\%) were determined using HPLC to quantitatively assess the RA in supernatants. Results showed that Witepsol H15 produced nanoparticles with initial mean diameters between 270 and 1000nm, yet over time, a slight increase occurred, but without occurrence of aggregation. The AE\% showed a high percentage of encapsulation (ca. 99\%), which reveals low polyphenol releases from SLNs throughout storage time. In general, results showed a successful production of SLNs with properties that can be used to food applications. {\textcopyright} 2013 Elsevier B.V.},
	keywords = {Optimization, Rosmarinic acid, SLNs, Witepsol},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84892548596\&partnerID=40\&md5=231718083d6238d5ac676d642e12b0f8},
	author = {Campos, D.A. and Madureira, A.R. and Gomes, A.M. and Sarmento, B. and Pintado, M.M.}
}
@article {3289,
	title = {The potential utility of chitosan micro/nanoparticles in the treatment of gastric infection},
	journal = {Expert Review of Anti-Infective Therapy},
	volume = {12},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {981 - 992},
	abstract = {Gastric infections are mainly caused by Helicobacter pylori (H. pylori), a bacterium that colonizes the gastric mucosa of over 50\% of the world{\textquoteright}s population. Chronic H. pylori infection has been associated with gastric diseases such as chronic gastritis, peptic ulcer and gastric adenocarcinoma. Current eradication treatment relies on antibiotic-based therapies that are unsuccessful in approximately 20\% of the patients. Chitosan, a natural and cationic polysaccharide has been investigated in the treatment of H. pylori infection. Due to its mucoadhesive properties, it has been used in the form of micro/nanoparticles, polyelectrolyte complexes or coatings as antibiotic encapsulation systems for gastric delivery, but alternative molecules may also be incorporated. It has been recently proposed that chitosan can also be used for H. pylori binding and scavenging from the host stomach due to its antimicrobial/binding properties. In this manuscript, a brief description of the use of chitosan in H. pylori treatment is reviewed. {\textcopyright} Informa UK, Ltd.},
	keywords = {Biomaterials, chitosan, Crosslinking, Drug delivery, Gastric cancer, Helicobacter pylori, Microspheres, mucoadhesion, nanoparticles, Polyelectrolytes},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904003586\&partnerID=40\&md5=d8a682d7ce6be8633b1505d0a7b8adae},
	author = {Gon{\c c}alves, I.C. and Henriques, P.C. and Seabra, C.L. and Martins, M.C.L.}
}
@article {3301,
	title = {Primary bone marrow mesenchymal stromal cells rescue the axonal phenotype of twitcher mice},
	journal = {Cell Transplantation},
	volume = {23},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {239 - 252},
	abstract = {Krabbe{\textquoteright}s disease (KD) is a demyelinating disorder caused by the deficiency of lysosomal galactocerebrosidase (GALC), affecting both the central (CNS) and the peripheral nervous system (PNS). A current therapy, hematopoietic stem cell transplantation (HSCT), is ineffective at correcting the PNS pathology. We have previously shown that systemic delivery of immortalized bone marrow-derived murine mesenchymal stromal cells (BM-MSCs) diminishes the neuropathology of transplanted Twitcher mice, a murine model of KD. In this study, to move one step closer to clinical application, the effectiveness of a systematic delivery of primary BM-MSCs to promote recovery of the Twitcher PNS was assessed. Primary BM-MSCs grafted to the Twitcher sciatic nerve led to increased GALC activity that was not correlated to decreased psychosine (the toxic GALC substrate) accumulation. Nevertheless, BM-MSC transplantation rescued the axonal phenotype of Twitcher mice in the sciatic nerve, with an increased density of both myelinated and unmyelinated axons in transplanted animals. Whereas no increase in myelination was observed, upon transplantation an increased proliferation of Schwann cell precursors occurred. Supporting these findings, in vitro, BM-MSCs promoted neurite outgrowth of Twitcher sensory neurons and proliferation of Twitcher Schwann cells. Moreover, BM-MSCs expressed nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) and promoted increased BDNF synthesis by neighboring Schwann cells. Besides their action in neurons and glia, BM-MSCs led to macrophage activation in Twitcher sciatic nerves. In summary, primary BM-MSCs diminish the neuropathology of Twitcher sciatic nerves by coordinately affecting neurons, glia, and macrophages. {\textcopyright} 2014 Cognizant Comm. Corp.},
	keywords = {Axonal degeneration, Bone marrow-derived mesenchymal stromal cells (BM-MSCs), Krabbe{\textquoteright}s disease (KD), Twitcher mice},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84893339069\&partnerID=40\&md5=51c17895a58b06f3b302616459dff62b},
	author = {Miranda, C.O. and Teixeira, C.A. and Sousa, V.F. and Santos,TE and Liz, M.A. and Marques,AM and Pinto-Do-{\'O}, P. and Sousa, M.M.}
}
@article {3277,
	title = {Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: The sea urchin Paracentrotus lividus},
	journal = {Marine Drugs},
	volume = {12},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {4912 - 4933},
	abstract = {Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is required, and marine organisms are among the safest and recently exploited ones. Sea urchins possess a circular area of soft tissue surrounding the mouth, the peristomial membrane (PM), mainly composed by mammalian-like collagen. The PM of the edible sea urchin Paracentrotus lividus therefore represents a potential unexploited collagen source, easily obtainable as a food industry waste product. Our results demonstrate that it is possible to extract native collagen fibrils from the PM and produce suitable substrates for in vitro system. The obtained matrices appear as a homogeneous fibrillar network (mean fibril diameter 30-400 nm and < 2 μm) and display remarkable mechanical properties in term of stiffness (146 {\textpm} 48 MPa) and viscosity (60.98 {\textpm} 52.07 GPa{\textperiodcentered}s). In vitro tests with horse pbMSC show a good biocompatibility in terms of overall cell growth. The obtained results indicate that the sea urchin P. lividus can be a valuable low-cost collagen source for mechanically resistant biomedical devices.},
	keywords = {Biocompatibility, Collagen matrix, Mesenchimal stromal cells, Native fibril, Sea urchin collagen, Tissue regeneration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84908025685\&partnerID=40\&md5=58774d56d0d60e6bc725ae6ecacaa068},
	author = {Di Benedetto, C. and Barbaglio, A. and Martinello, T. and Alongi, V. and Fassini, D. and Cullor{\`a}, E. and Patruno, M. and Bonasoro, F. and Barbosa, M.A. and Carnevali, M.D.C. and Sugni, M.}
}
@article {3276,
	title = {Propolis varnish: Antimicrobial properties against cariogenic bacteria, cytotoxicity, and sustained-release profile},
	journal = {BioMed Research International},
	volume = {2014},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Varnishes are preparations that differ in the polymeric matrix and therapeutical agents. In dentistry they are used to prevent caries. In this study we developed a propolis varnish, considering propolis properties against cariogenic bacteria. To a chitosan polymeric base (CHV) was added ethanolic propolis extract in different concentrations: PV1 (5\%), PV2 (10\%), and PV3 (15\%). Antimicrobial activity was carried out against Streptococcus mutans (SM), Streptococcus sanguinis (SG), Streptococcus salivarius (SS), and Lactobacillus casei (LC) through agar diffusion method. The three propolis concentrations incorporated were effective in inhibiting the growth of all microorganisms, but without significant difference between the zones of inhibition observed. Cytotoxicity assay was done by MTT method. Data were analyzed by one-way ANOVA and Bonferroni test. None of the varnishes were cytotoxic, keeping 80\% of viable cells, while CHV allowed cellular proliferation (120\%). Sustained-release test was carried out by applying 40 μL of each varnish in the buccal surface of bovine teeth and kept in an ethanol/water solution removed in regular times. According to the "independent model approach," the release profiles were distinct from each varnish and the most prolonged was PV3 (8 weeks). Varnish formulations had satisfactory antimicrobial activity against cariogenic bacteria and have a low cytotoxicity (<50\%). {\textcopyright} 2014 Mariana P. De Luca et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84901784340\&partnerID=40\&md5=3324d6156f7606cd3ee0a5d55e0ee107},
	author = {De Luca, M.P. and Franca, J.R. and Macedo, F.A.F.F. and Grenho, L. and Cortes, M.E. and Faraco, A.A.G. and Moreira, A.N. and Santos, V.R.}
}
@article {3267,
	title = {Rescue of wild-type E-cadherin expression from nonsense-mutated cancer cells by a suppressor-tRNA},
	journal = {European Journal of Human Genetics},
	volume = {22},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1085 - 1092},
	abstract = {Hereditary diffuse gastric cancer (HDGC) syndrome, although rare, is highly penetrant at an early age, and is severe and incurable because of ineffective screening tools and therapy. Approximately 45\% of HDGC families carry germline CDH1/E-cadherin alterations, 20\% of which are nonsense leading to premature protein truncation. Prophylactic gastrectomy is the only recommended approach for all asymptomatic CDH1 mutation carriers. Suppressor-tRNAs can replace premature stop codons (PTCs) with a cognate amino acid, inducing readthrough and generating full-length proteins. The use of suppressor-tRNAs in HDGC patients could therefore constitute a less invasive therapeutic option for nonsense mutation carriers, delaying the development of gastric cancer. Our analysis revealed that 23/108 (21.3\%) of E-cadherin-mutant families carried nonsense mutations that could be potentially corrected by eight suppressor-tRNAs, and arginine was the most frequently affected amino acid. Using site-directed mutagenesis, we developed an arginine suppressor-tRNA vector to correct one HDGC nonsense mutation. E-cadherin-deficient cell lines were transfected with plasmids carrying simultaneously the suppressor-tRNA and wild-type or mutant CDH1 mini-genes. RT-PCR, western blot, immunofluorescence, flow cytometry and proximity ligation assay (PLA) were used to establish the model, and monitor mRNA and protein expression and function recovery from CDH1 vectors. Cells expressing a CDH1 mini-gene, carrying a nonsense mutation and the suppressor-tRNA, recovered full-length E-cadherin expression and its correct localization and incorporation into the adhesion complex. This is the first demonstration of functional recovery of a mutated causative gene in hereditary cancer by cognate amino acid replacement with suppressor-tRNAs. Of the HDGC families, 21.3\% are candidates for correction with suppressor-tRNAs to potentially delay cancer onset. {\textcopyright} 2014 Macmillan Publishers Limited. All rights reserved.},
	keywords = {hereditary diffuse gastric cancer (HDGC), nonsense suppression, Premature termination codon (PTC), Suppressor-tRNA},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84906269658\&partnerID=40\&md5=2feed844660a13385857a7e5d4470a2b},
	author = {Bordeira-Carri{\c c}o, R. and Ferreira, D. and Mateus, D.D. and Pinheiro, H. and P{\^e}go, A.P. and Santos, M.A.S. and Oliveira, C.}
}
@article {3327,
	title = {Role of protein environment and bioactive polymer grafting in the S. epidermidis response to titanium alloy for biomedical applications},
	journal = {Materials Science and Engineering C},
	volume = {45},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {176 - 183},
	abstract = {Joint implant-related infections, namely by Staphylococci, are a worldwide problem, whose consequences are dramatic. Various methods are studied to fight against these infections. Here, the proposed solution consists in grafting a bioactive polymer on joint implant surfaces in order to allow the control of the interactions with the living system. In this study, sodium styrene sulfonate, bearing sulfonate groups, was grafted on the surface of titanium alloys. Scanning Electron Microscopy, colorimetric method, Fourier-transformed infrared spectroscopy and contact angle measurements were applied to characterize the surfaces. Bacterial adhesion studies were studied on poly(sodium styrene sulfonate) grafted Ti6Al4V and Ti6Al4V surfaces previously adsorbed by proteins involved in the bacteria adhesion process. Fibrinogen and fibronectin were demonstrated to increase staphylococcal adhesion on Ti6Al4V surfaces. Ti6Al4V grafted sodium styrene sulfonate surfaces inhibited the adhesion of Staphylococcus epidermidis in 37\% and 13\% on pre-adsorbed surfaces with fibrinogen and fibronectin, respectively. The mechanism of the observed inhibiting bacteria adhesion properties is related to the differences of proteic conformations induced by poly(sodium styrene sulfonate) grafting.},
	keywords = {Bacterial adhesion, Bioactive polymer, Poly(sodium styrene sulfonate) (polyNaSS), Protein adsorption, Surface modification, Titanium alloy},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84907482809\&partnerID=40\&md5=21d4fdd30e05852f6b8ce9a0dcf846cf},
	author = {Vasconcelos, D.M. and Falentin-Daudr{\'e}, C. and Blanquaert, D. and Thomas, D. and Granja, P.L. and Migonney, V.}
}
@article {3315,
	title = {Role of sparc in bone remodeling and cancer-related bone metastasis},
	journal = {Journal of Cellular Biochemistry},
	volume = {115},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {17 - 26},
	abstract = {There is a growing socioeconomic recognition that clinical bone diseases such as bone infections, bone tumors and osteoporotic bone loss mainly associated with ageing, are major issues in today{\textquoteright}s society. SPARC (secreted protein, acidic and rich in cysteine), a matricellular glycoprotein, may be a promising therapeutic target for preventing or treating bone-related diseases. In fact, SPARC is associated with tissue remodeling, repair, development, cell turnover, bone mineralization and may also participate in growth and progression of tumors, namely cancer-related bone metastasis. Yet, the function of SPARC in such biological processes is poorly understood and controversial. The main objective of this work is to review the current knowledge related to the activity of SPARC in bone remodeling, tumorigenesis, and bone metastasis. Progress in understanding SPARC biology may provide novel strategies for bone regeneration and the development of anti-angiogenic, anti-proliferative, or counter-adhesive treatments specifically against bone metastasis. {\textcopyright} 2013 Wiley Periodicals, Inc. {\textcopyright} 2013 Wiley Periodicals, Inc.},
	keywords = {BONE METASTASIS, bone remodeling, SPARC, TUMORIGENESIS},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84887856025\&partnerID=40\&md5=bcb4dc43db0bb9be75051f4493c74d3d},
	author = {Ribeiro, N. and Sousa, S.R. and Brekken, R.A. and Monteiro, F.J.}
}
@article {3285,
	title = {Selective albumin-binding surfaces modified with a thrombin-inhibiting peptide},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1227 - 1237},
	abstract = {Blood-contacting medical devices have been associated with severe clinical complications, such as thrombus formation, triggered by the activation of the coagulation cascade due to the adsorption of certain plasma proteins on the surface of biomaterials. Hence, the coating of such surfaces with antithrombotic agents has been used to increase biomaterial haemocompatibility. Biomaterial-induced clotting may also be decreased by albumin adsorption from blood plasma in a selective and reversible way, since this protein is not involved in the coagulation cascade. In this context, this paper reports that the immobilization of the thrombin inhibitor D-Phe-Pro-D-Arg-D-Thr-CONH 2 (fPrt) onto nanostructured surfaces induces selective and reversible adsorption of albumin, delaying the clotting time when compared to peptide-free surfaces. fPrt, synthesized with two glycine residues attached to the N-terminus (GGfPrt), was covalently immobilized onto self-assembled monolayers (SAMs) having different ratios of carboxylate-hexa(ethylene glycol)- and tri(ethylene glycol)-terminated thiols (EG6-COOH/EG3) that were specifically designed to control GGfPrt orientation, exposure and density at the molecular level. In solution, GGfPrt was able to inactivate the enzymatic activity of thrombin and to delay plasma clotting time in a concentration-dependent way. After surface immobilization, and independently of its concentration, GGfPrt lost its selectivity to thrombin and its capacity to inhibit thrombin enzymatic activity against the chromogenic substrate n-p-tosyl-Gly-Pro-Arg-p-nitroanilide. Nevertheless, surfaces with low concentrations of GGfPrt could delay the capacity of adsorbed thrombin to cleave fibrinogen. In contrast, GGfPrt immobilized in high concentrations was found to induce the procoagulant activity of the adsorbed thrombin. However, all surfaces containing GGfPrt have a plasma clotting time similar to the negative control (empty polystyrene wells), showing resistance to coagulation, which is explained by its capacity to adsorb albumin in a selective and reversible way. This work opens new perspectives to the improvement of the haemocompatibility of blood-contacting medical devices. {\textcopyright} 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Albumin, Blood compatibility, Clotting, Surface modification, Thrombin},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84895067841\&partnerID=40\&md5=8486186625f8f4f1cbc538bb3e34dd82},
	author = {Freitas, S.C. and Maia, S. and Figueiredo, A.C. and Gomes, P. and Pereira, P.J.B. and Barbosa, M.A. and Martins, M.C.L.}
}
@article {3398,
	title = {Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model},
	journal = {Journal of Clinical Investigation},
	volume = {124},
	year = {2014},
	note = {Bestas, Burcu Moreno, Pedro M. D. Blomberg, K. Emelie M. Mohammad, Dara K. Saleh, Amer F. Sutlu, Tolga Nordin, Joel Z. Guterstam, Peter Gustafsson, Manuela O. Kharazi, Shabnam Piatosa, Barbara Roberts, Thomas C. Behlke, Mark A. Wood, Matthew J. A. Gait, Michael J. Lundin, Karin E. El Andaloussi, Samir Mansson, Robert Berglof, Anna Wengel, Jesper Smith, C. I. Edvard},
	month = {2014},
	pages = {4067-4081},
	isbn = {0021-9738},
	url = {<Go to ISI>://WOS:000341168100038},
	author = {Bestas, B. and Moreno, P.M.D. and Blomberg, K. E. M. and Mohammad, D. K. and Saleh, A. F. and Sutlu, T. and Nordin, J. Z. and Guterstam, P. and Gustafsson, M. O. and Kharazi, S. and Piatosa, B. and Roberts, T. C. and Behlke, M. A. and Wood, M. J. A. and Gait, M. J. and Lundin, K.E. and El Andaloussi, S. and Mansson, R. and Berglof, A. and Wengel, J. and Smith, C.I.E.}
}
@article {3407,
	title = {Therapeutic antisense oligonucleotides against cancer: hurdling to the clinic},
	journal = {Frontiers in chemistry},
	volume = {2},
	year = {2014},
	note = {25353019},
	month = {2014},
	pages = {87},
	isbn = {2296-2646},
	url = {<Go to ISI>://MEDLINE:25353019},
	author = {Moreno, Pedro M. D. and Pego, Ana P.}
}
@article {3417,
	title = {Tools for address georeferencing - limitations and opportunities every public health professional should be aware of},
	journal = {PloS one},
	volume = {9},
	year = {2014},
	month = {2014},
	pages = {e114130-e114130},
	url = {<Go to ISI>://MEDLINE:25469514},
	author = {Ribeiro, Ana Isabel and Olhero, Andreia and Teixeira, Hugo and Magalhaes, Alexandre and Pina, Maria Fatima}
}
@article {3404,
	title = {Translating chitosan to clinical delivery of nucleic acid-based drugs},
	journal = {Mrs Bulletin},
	volume = {39},
	year = {2014},
	note = {Gomes, Carla Pereira Ferreira Lopes, Catia Daniela Duarte Moreno, Pedro Miguel Varela-Moreira, Aida Jose Alonso, Maria Pego, Ana PaulaPego, Ana Paula/C-6942-2012; Moreno, Pedro/J-9382-2013; Lopes, Catia/O-1090-2013
Pego, Ana Paula/0000-0001-5169-328X; Moreno, Pedro/0000-0002-3773-3262; Lopes, Catia/0000-0002-7467-6498
},
	month = {2014},
	pages = {60-70},
	isbn = {0883-7694},
	url = {<Go to ISI>://WOS:000330833200016},
	author = {Gomes, C.P. and Lopes, C.D.F. and Moreno, P.M.D. and Varela-Moreira, A. and Alonso, M.J. and Pego, A. P.}
}
@article {3309,
	title = {What do Portuguese women prefer regarding vaginal products? Results from a cross-sectional web-based survey},
	journal = {Pharmaceutics},
	volume = {6},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {543 - 556},
	abstract = {Therapeutic outcomes of vaginal products depend not only on their ability to deliver drugs to or through the vagina but also on acceptability and correct use. Women{\textquoteright}s preferences, in turn, may vary according to age and cultural backgrounds. In this work, an anonymous online survey was completed by 2529 Portuguese women to assess their preferences for physical characteristics and mode of application of vaginal products, according to age. Additionally, intention to use and misconceptions about these issues were assessed. The majority of women of all age groups would use vaginal products to treat or prevent diseases, upon medical prescription. Women preferred vaginal products to be odorless and colorless gels, creams and ointments composed by natural origin drugs/excipients and applied by means of an applicator. Although the majority of women would prefer not to insert any product in the vagina, intention to use for self and recommendation to use for others was associated with previous experiences with vaginal products. General concerns and misconceptions related to use of vaginal products were rare. These data may contribute to the development of products that women are more prone to use.},
	keywords = {Acceptability, Vaginal dosage forms, Vaginal products, Women{\textquoteright}s preferences},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84908210757\&partnerID=40\&md5=5515e40e97ff157b0a5b4498557008ef},
	author = {Palmeira-de-Oliveira, R. and Duarte, P. and Palmeira-de-Oliveira, A. and Das Neves, J. and Amaral, M.H. and Breitenfeld, L. and Martinez-de-Oliveira, J.}
}
@article {3414,
	title = {What do portuguese women prefer regarding vaginal products? Results from a cross-sectional web-based survey},
	journal = {Pharmaceutics},
	volume = {6},
	year = {2014},
	note = {25337676},
	month = {2014},
	pages = {543-56},
	isbn = {1999-4923},
	url = {<Go to ISI>://MEDLINE:25337676},
	author = {Palmeira-de-Oliveira, Rita and Duarte, Paulo and Palmeira-de-Oliveira, Ana and das Neves, Jose and Amaral, Maria Helena and Breitenfeld, Luiza and Martinez-de-Oliveira, Jose}
}
@article {2759,
	title = {Adsorbed fibrinogen leads to improved bone regeneration and correlates with differences in the systemic immune response},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 23 May 2013Source: ScopusArticle in Press},
	month = {07/2013},
	pages = {7209-7217},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876834487\&partnerID=40\&md5=2d00f0bc55f461c9c979548276f0e53d},
	author = {Santos, S.G. and Lamghari, M. and Almeida, C.R. and Oliveira, M.I. and Neves, N. and Ribeiro, A.C. and Barbosa, J.N. and Barros, R. and Maciel, J. and Martins, M.C.L. and Gon{\c c}alves, R.M. and Barbosa, M.A.}
}
@article {2849,
	title = {Adult stem cells and biocompatible scaffolds as smart drug delivery tools for cardiac tissue repair},
	journal = {Current Medicinal Chemistry},
	volume = {20},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {3429 - 3447},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84883267656\&partnerID=40\&md5=b03789229cfac322e56bb69b4fc644ea},
	author = {Pagliari, S. and Romanazzo, S. and Mosqueira, D. and Pinto-Do-{\'O}, P. and Aoyagi, T. and Forte, G.}
}
@article {2842,
	title = {Automatic lane segmentation in TLC images using the continuous wavelet transform},
	journal = {Computational and Mathematical Methods in Medicine},
	volume = {2013},
	year = {2013},
	note = {Export Date: 12 December 2013Source: ScopusArt. No.: 218415},
	month = {2013///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84885671185\&partnerID=40\&md5=dd125f056cd63a76368b0ad6db6836c6},
	author = {Moreira, B. and Sousa, A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2855,
	title = {Automatic localization of the optic disc by combining vascular and intensity information},
	journal = {Computerized Medical Imaging and Graphics},
	volume = {37},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {409 - 417},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84888008484\&partnerID=40\&md5=10373bbd299a0e0b2a6890fe7fb1a69a},
	author = {Mendon{\c c}a, A.M. and Sousa, A. and Mendon{\c c}a, L. and Campilho, A.}
}
@booklet {2829,
	title = {Bacterial-binding chitosan microspheres for gastric infection treatment and prevention},
	howpublished = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {9370 - 9378},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84887160912\&partnerID=40\&md5=dd414f3b1e57bee7c37bc37a44983d27},
	author = {Gon{\c c}alves, I.C. and Magalh{\~a}es, A. and Fernandes, M. and Rodrigues, I.V. and Reis, C.A. and Martins, M.C.L.}
}
@article {2872,
	title = {Biocompatibility of poly(lactic acid) with incorporated graphene-based materials},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {104},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {229 - 238},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84872444845\&partnerID=40\&md5=7d61d767da7f0776cca70616b06301a9},
	author = {Pinto, A.M. and Moreira, S. and Gon{\c c}alves, I.C. and Gama, F.M. and Mendes, A.M. and Magalh{\~a}es, F.D.}
}
@article {2835,
	title = {Bioengineered surfaces promote specific protein-glycan mediated binding of the gastric pathogen Helicobacter pylori},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {8885 - 8893},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84885052038\&partnerID=40\&md5=0d17eb0f95d76eb18ea20ef7f33c8192},
	author = {Parreira, P. and Magalh{\~a}es, A. and Reis, C.A. and Bor{\'e}n, T. and Leckband, D. and Martins, M.C.L.}
}
@article {2836,
	title = {Brain targeting effect of camptothecin-loaded solid lipid nanoparticles in rat after intravenous administration},
	journal = {European Journal of Pharmaceutics and Biopharmaceutics},
	volume = {85},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {488 - 502},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84888387689\&partnerID=40\&md5=e40e0a0c4221bfbc77f793ace6e47dcd},
	author = {Martins, S.M. and Sarmento, B. and Nunes, C. and L{\'u}cio, M. and Reis, S. and Ferreira, D.C.}
}
@article {2869,
	title = {Cetuximab conjugated O-carboxymethyl chitosan nanoparticles for targeting EGFR overexpressing cancer cells},
	journal = {Carbohydrate Polymers},
	volume = {93},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {661 - 669},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84874136100\&partnerID=40\&md5=71075b389cbeb450f68caf7cbb271c08},
	author = {Maya, S. and Kumar, L.G. and Sarmento, B. and Sanoj Rejinold, N. and Menon, D. and Nair, S.V. and Jayakumar, R.}
}
@article {2878,
	title = {Development of bis-locked nucleic acid (bisLNA) oligonucleotides for efficient invasion of supercoiled duplex DNA},
	journal = {Nucleic Acids Research},
	volume = {41},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {3257 - 3273},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876401355\&partnerID=40\&md5=96078ee73fe175f8f962db91c20a8724},
	author = {Moreno, P.M.D. and Geny, S. and Pabon, Y.V. and Bergquist, H. and Zaghloul, E.M. and Rocha, C.S.J. and Oprea, I.I. and Bestas, B. and Andaloussi, S.E. and J{\o}rgensen, P.T. and Pedersen, E.B. and Lundin, K.E. and Zain, R. and Wengel, J. and Smith, C.I.E.}
}
@article {2879,
	title = {E-cadherin and adherens-junctions stability in gastric carcinoma: Functional implications of glycosyltransferases involving N-glycan branching biosynthesis, N-acetylglucosaminyltransferases III and v},
	journal = {Biochimica et Biophysica Acta - General Subjects},
	volume = {1830},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {2690 - 2700},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873589547\&partnerID=40\&md5=ea238ee2045a2bbfb910b24526aac863},
	author = {Pinho, S.S. and Figueiredo, J. and Cabral, J. and Carvalho, S. and Dourado, J. and Magalh{\~a}es, A. and G{\"a}rtner, F. and Mendon{\c c}a, A.M. and Isaji, T. and Gu, J. and Carneiro, F. and Seruca, R. and Taniguchi, N. and Reis, C.A.}
}
@article {2885,
	title = {Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {5208 - 5215},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84872069015\&partnerID=40\&md5=22eea6321dd7764e20a4460b47a72ba8},
	author = {Nogueira, F. and Gon{\c c}alves, I.C. and Martins, M.C.L.}
}
@article {2856,
	title = {Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration - In vitro study},
	journal = {Journal of Biomedical Materials Research - Part B Applied Biomaterials},
	volume = {101 B},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {762 - 769},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84878874447\&partnerID=40\&md5=edd0491ee0c1e8faf857a483e14efaf9},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Carvalho, A. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
@article {2876,
	title = {Enzymatic, physicochemical and biological properties of MMP-sensitive alginate hydrogels},
	journal = {Soft Matter},
	volume = {9},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {3283 - 3292},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84874428389\&partnerID=40\&md5=39d83a72fc608cc7f1c875e612421ff5},
	author = {Fonseca, K.B. and Maia, F.R. and Cruz, F.A. and Andrade, D. and Juliano, M.A. and Granja, P.L. and Barrias, C.C.}
}
@article {2838,
	title = {Functionalization of biomaterials with small osteoinductive moieties},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {8773 - 8789},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84885024380\&partnerID=40\&md5=a9ee12bc1c5789cd64861fd8201fc645},
	author = {Maia, F.R. and Bidarra, S.J. and Granja, P.L. and Barrias, C.C.}
}
@article {2839,
	title = {Graphene-based materials biocompatibility: A review},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {111},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {188 - 202},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879808520\&partnerID=40\&md5=ae9e0dbaf79ff79a5f60800ac798b26d},
	author = {Pinto, A.M. and Gon{\c c}alves, I.C. and Magalh{\~a}es, F.D.}
}
@article {2862,
	title = {High biocompatibility and improved osteogenic potential of novel Ca-P/titania composite scaffolds designed for regeneration of load-bearing segmental bone defects},
	journal = {Journal of Biomedical Materials Research - Part A},
	volume = {101 A},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1612 - 1619},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876296528\&partnerID=40\&md5=6721f219742ba18cfd907ca516ddcd07},
	author = {Cunha, C. and Sprio, S. and Panseri, S. and Dapporto, M. and Marcacci, M. and Tampieri, A.}
}
@article {2866,
	title = {Hyaluronic acid / chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes},
	journal = {Journal of Controlled Release},
	volume = {168},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {289 - 297},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877033262\&partnerID=40\&md5=d58ff56593b3a791351102271ca61440},
	author = {Hartmann, H. and Hossfeld, S. and Schlosshauer, B. and Mittnacht, U. and P{\^e}go, A.P. and Dauner, M. and Doser, M. and Stoll, D. and Krastev, R.}
}
@article {2863,
	title = {Hydrolyzed galactomannan-modified nanoparticles and flower-like polymeric micelles for the active targeting of rifampicin to macrophages},
	journal = {Journal of Biomedical Nanotechnology},
	volume = {9},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1076 - 1087},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877815633\&partnerID=40\&md5=d4bbcc67f9930d53201ec08dbcfce826},
	author = {Moretton, M.A. and Chiappetta, D.A. and Andrade, F. and Das Neves, J. and Ferreira, D. and Sarmento, B. and Sosnik, A.}
}
@article {2857,
	title = {In vitro and Ex Vivo evaluation of polymeric nanoparticles for vaginal and rectal delivery of the anti-HIV drug dapivirine},
	journal = {Molecular Pharmaceutics},
	volume = {10},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {2793 - 2807},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879736868\&partnerID=40\&md5=cd7771212c94b7031331e338743b05ed},
	author = {Das Neves, J. and Ara{\'u}jo, F. and Andrade, F. and Michiels, J. and Ari{\"e}n, K.K. and Vanham, G. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2877,
	title = {Information-based measure of disagreement for more than two observers: A useful tool to compare the degree of observer disagreement},
	journal = {BMC Medical Research Methodology},
	volume = {13},
	year = {2013},
	note = {Export Date: 12 December 2013Source: ScopusArt. No.: 47},
	month = {2013///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84875159444\&partnerID=40\&md5=c62818fbdde48aa73825e4780acb6f70},
	author = {Henriques, T. and Antunes, L. and Bernardes, J. and Matias, M. and Sato, D. and Costa-Santos, C.}
}
@article {2864,
	title = {Keratins and lipids in ethnic hair},
	journal = {International Journal of Cosmetic Science},
	volume = {35},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {244 - 249},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877800810\&partnerID=40\&md5=2064f5beb8715287759479c528eaeac1},
	author = {Cruz, C.F. and Fernandes, M.M. and Gomes, A.C. and Coderch, L. and Mart{\'\i}, M. and M{\'e}ndez, S. and Gales, L. and Azoia, N.G. and Shimanovich, U. and Cavaco-Paulo, A.}
}
@article {2889,
	title = {Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness},
	journal = {Human Molecular Genetics},
	volume = {22},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: ScopusArt. No.: dds405},
	month = {2013///},
	pages = {84 - 95},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84871200220\&partnerID=40\&md5=b2bfd70fb2a8a4e517e4ad698a2461cc},
	author = {Moniz, S. and Martinho, O. and Pinto, F. and Sousa, B. and Loureiro, C. and Oliveira, M.J. and Moita, L.F. and Honavar, M. and Pinheiro, C. and Pires, M. and Lopes, J.M. and Jones, C. and Costello, J.F. and Paredes, J. and Reis, R.M. and Jordan, P.}
}
@booklet {2867,
	title = {Macrophages stimulate gastric and colorectal cancer invasion through EGFR Y1086, c-Src, Erk1/2 and Akt phosphorylation and smallGTPase activity},
	howpublished = {Oncogene},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: ScopusArticle in Press},
	month = {2013///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876908646\&partnerID=40\&md5=bcc2929bb93817db3dc0e3421c420722},
	author = {Cardoso, A.P. and Pinto, M.L. and Pinto, A.T. and Oliveira, M.I. and Pinto, M.T. and Gon{\c c}alves, R. and Relvas, J.B. and Figueiredo, C. and Seruca, R. and Mantovani, A. and Mareel, M. and Barbosa, M.A. and Oliveira, M.J.}
}
@book {2890,
	title = {Minimum error entropy classification},
	series = {Studies in Computational Intelligence},
	volume = {420},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1 - 278},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867489006\&partnerID=40\&md5=45e5679e51ca7e192b098b2320ec561a},
	author = {Marques De S{\'a}, J.P. and Silva, L.M.A. and Santos, J.M.F. and Alexandre, L.A.}
}
@article {2870,
	title = {A model for educational simulation of the effect of oxytocin on uterine contractions},
	journal = {Medical Engineering and Physics},
	volume = {35},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {524 - 531},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876114966\&partnerID=40\&md5=ac7aee56840fe71fcaaa1171d75e927c},
	author = {Lobo, M.F. and Bastos, L.F. and van Meurs, W.L. and Ayres-de-Campos, D.}
}
@article {2847,
	title = {Modulation of stability and mucoadhesive properties of chitosan microspheres for therapeutic gastric application},
	journal = {International Journal of Pharmaceutics},
	volume = {454},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {116 - 124},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84884163785\&partnerID=40\&md5=1d040067777b023c63bb769260b66dcd},
	author = {Fernandes, M. and Gon{\c c}alves, I.C. and Nardecchia, S. and Amaral, I.F. and Barbosa, M.A. and Martins, M.C.L.}
}
@article {2843,
	title = {Neuropeptide y modulates fracture healing through Y1 receptor signaling},
	journal = {Journal of Orthopaedic Research},
	volume = {31},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1570 - 1578},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84882886747\&partnerID=40\&md5=dd7576a200a42ce37ff5a98115fe9462},
	author = {Sousa, D.M. and McDonald, M.M. and Mikulec, K. and Peacock, L. and Herzog, H. and Lamghari, M. and Little, D.G. and Baldock, P.A.}
}
@article {2886,
	title = {Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability},
	journal = {International Journal of Nanomedicine},
	volume = {8},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {177 - 187},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84872351206\&partnerID=40\&md5=fc5d64a3ada5d6918b225c107d60049e},
	author = {Neves, A.R. and L{\'u}cio, M. and Martins, S. and Lima, J.L.C. and Reis, S.}
}
@article {2840,
	title = {Physical activity-friendly neighbourhood among older adults from a medium size urban setting in Southern Europe},
	journal = {Preventive Medicine},
	volume = {57},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {664 - 670},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84885858460\&partnerID=40\&md5=758283ae99e1a87106c231876949b707},
	author = {Ribeiro, A.I. and Mitchell, R. and Carvalho, M.S. and De Pina, M.D.F.}
}
@article {2875,
	title = {Preparation and characterization of collagen-nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications},
	journal = {Journal of Biomedical Materials Research - Part A},
	volume = {101 A},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1080 - 1094},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876183449\&partnerID=40\&md5=2f1dd0c83479a986914ef33f66141939},
	author = {Rodrigues, S.C. and Salgado, C.L. and Sahu, A. and Garcia, M.P. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {2891,
	title = {Response of monocultured and co-cultured human microvascular endothelial cells and mesenchymal stem cells to macroporous granules of nanostructured- hydroxyapatite agglomerates},
	journal = {Journal of Biomedical Nanotechnology},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1594 - 1606},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84883350324\&partnerID=40\&md5=d78750836664276ab71c67301dab9c83},
	author = {Laranjeira, M.S. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {2831,
	title = {Sociodemographic determinants of prevalence and incidence of helicobacter pylori infection in portuguese adults},
	journal = {Helicobacter},
	volume = {18},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {413 - 422},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84886298522\&partnerID=40\&md5=460550c9c4474e105c93815a934c98ee},
	author = {Bastos, J. and Peleteiro, B. and Barros, R. and Alves, L. and Severo, M. and de F{\'a}tima Pina, M. and Pinto, H. and Carvalho, S. and Marinho, A. and Guimar{\~a}es, J.T. and Azevedo, A. and La Vecchia, C. and Barros, H. and Lunet, N.}
}
@article {2205,
	title = {Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa onto nanohydroxyapatite as a bone regeneration material},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {100 A},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.34139PubMed ID: 22489063Language of Original Document: EnglishCorrespondence Address: Grenho, L.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: liliana.grenho@ineb.up.ptChemicals/CAS: Biocompatible Materials; Durapatite, 1306-06-5References: Darley, E.S.R., MacGowan, A.P., Antibiotic treatment of Gram-positive bone and joint infections (2004) J Antimicrob Chemother, 53, pp. 928-935;Charville, G.W., Hetrick, E.M., Geer, C.B., Schoenfisch, M.H., Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release (2008) Biomaterials, 29, pp. 4039-4044; 
Katsikogianni, M., Amanatides, E., Mataras, D., Missirlis, Y.F., Staphylococcus epidermidis adhesion to He, He/O(2) plasma treated PET films and aged materials: Contributions of surface free energy and shear rate (2008) Colloids Surf B Biointerfaces, 65, pp. 257-268; 
Katsikogianni, M., Missirlis, Y.F., Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions (2004) Eur Cell Mater, 8, pp. 37-57; 
Poelstra, K.A., Barekzi, N.A., Rediske, A.M., Felts, A.G., Slunt, J.B., Grainger, D.W., Prophylactic treatment of gram-positive and gram-negative abdominal implant infections using locally delivered polyclonal antibodies (2002) J Biomed Mater Res, 60, pp. 206-215; 
Pascual, A., Pathogenesis of catheter-related infections: Lessons for new designs (2002) Clin Microbiol Infect, 8, pp. 256-264; 
Katsikogianni, M., Spiliopoulou, I., Dowling, D.P., Missirlis, Y.F., Adhesion of slime producing Staphylococcus epidermidis strains to PVC and diamond-like carbon/silver/fluorinated coatings (2006) J Mater Sci Mater Med, 17, pp. 679-689; 
Donlan, R.M., Role of biofilms in antimicrobial resistance (2000) Asaio J, 46, pp. S47-S52; 
Donlan, R.M., Biofilms and device-associated infections (2001) Emerg Infect Dis, 7, pp. 277-281; 
Donlan, R.M., Biofilms: Microbial life on surfaces (2002) Emerg Infect Dis, 8, pp. 881-890; 
Ferraz, M.P., Monteiro, F.J., Giao, D., Leon, B., Gonzalez, P., Liste, S., Serra, J., Perez-Amor, M., CaO-P 2O 5 glass-hydroxyapatite thin films obtained by laser ablation: Characterisation and in vitro bioactivity evaluation (2004) Bioceramics, 16, pp. 347-350; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.D., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80; 
Mateus, A.Y.P., Barrias, C.C., Ribeiro, C.C., Ferraz, M.P., Monteiro, F.J., Comparative study of nanohydroxyapatite microspheres for medical applications (2008) J Biomed Mater Res A, 86, pp. 483-493; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci Mater Med, 19, pp. 855-859; 
Ferraz, M.P., Mateus, A.Y., Sousa, J.C., Monteiro, F.J., Nano-hydroxyapatite microspheres as delivery system for antibiotics: Release kinetics, antimicrobial activity and interaction with osteoblasts (2007) J Biomed Mater Res A, 81, pp. 994-1004; 
Oliveira, G.M., Ferraz, M.P., Gonz{\'a}lez, P., Serra, J., Leon, B., P{\`e}rez-Amor, M., Monteiro, F.J., PLD bioactive ceramic films: The influence of CaO-P2O5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells (2008) J Mater Sci Mater Med, 19, pp. 1775-1785; 
Ribeiro, N., Sousa, S.R., Monteiro, F.J., Influence of crystallite size of nanophased hydroxyapatite on fibronectin and osteonectin adsorption and on MC3T3-E1 osteoblast adhesion and morphology (2010) J Colloid Interface Sci, 351, pp. 398-406; 
Gadelmawla, E.S., Koura, M.M., Maksoud, T.M.A., Elewa, I.M., Soliman, H.H., Roughness parameters (2002) J Mater Proc Technol, 123, pp. 133-145; 
Campoccia, D., Montanaro, L., Arciola, C.R., The significance of infection related to orthopedic devices and issues of antibiotic resistance (2006) Biomaterials, 27, pp. 2331-2339; 
Muller, R., Groger, G., Hiller, K.A., Schmalz, G., Ruhl, S., Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria (2007) Appl Environ Microbiol, 73, pp. 2653-2660; 
Boyd, R.D., Verran, J., Jones, M.V., Bhakoo, M., Use of the atomic force microscope to determine the effect of substratum surface topography on bacterial adhesion (2002) Langmuir, 18, pp. 2343-2346; 
Fonseca, A.P., Granja, P.L., Nogueira, J.A., Oliveira, D.R., Barbosa, M.A., Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives (2001) J Mater Sci Mater Med, 12, pp. 543-548; 
Papaioannou, W., Quirynen, M., Vansteenberghe, D., The influence of periodontitis on the subgingival flora around implants in partially edentulous patients (1996) Clin Oral Implants Res, 7, pp. 405-409; 
Kamusewitz, H., Possart, W., Wetting and scanning force microscopy on rough polymer surfaces: Wenzel{\textquoteright}s roughness factor and the thermodynamic contact angle (2003) Appl Phys A Mater Sci Proc, 76, pp. 899-902; 
Katsikogianni, M.G., Missirlis, Y.F., Interactions of bacteria with specific biomaterial surface chemistries under flow conditions (2010) Acta Biomater, 6, pp. 1107-1118; 
Bazaka, K., Jacob, M.V., Crawford, R.J., Ivanova, E.P., Plasma-assisted surface modification of organic biopolymers to prevent bacterial attachment (2011) Acta Biomater, 7, pp. 2015-2028; 
Gotz, F., Staphylococcus and biofilms (2002) Mol Microbiol, 43, pp. 1367-1378
},
	month = {2012///},
	pages = {1823 - 1830},
	abstract = {In orthopedics due to the enormous number of surgical procedures involving invasive implant biomaterials, infections have a huge impact in terms of morbidity, mortality, and medical costs. In this study the initial adhesion of several strains namely Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa, to nanohydroxyapatite, previously heat-treated at 725{\textdegree}C and 1000{\textdegree}C was assessed. Adherent cells were evaluated by scanning electron microscopy and quantified by confocal laser scanning microscopy and as colony forming units after being released by sonication. The wettability and roughness of samples surfaces were assessed by contact angle measurements and atomic force microscopy, respectively. Nanohydroxyapatite heat-treated at 1000{\textdegree}C appeared to be more resistant to bacterial adhesion, over time, in five of the six tested strains while the clinical strains isolated from orthopedic infections presented superior ability to adhere, as well as better capacity to produce slime. The increase in materials sintering temperature resulted in increased hydrophobicity and roughness; however, other surface features such as the decrease in surface area and on porosity as well as the decrease on zeta potential may be the aspects that contributed to a lower bacterial adhesion on the materials sintered at 1000{\textdegree}C. {\textcopyright} 2012 Wiley Periodicals, Inc.},
	keywords = {Adherent cells, Adhesion, article, Atomic force microscopy, Bacteria, Bacterial adhesion, bacterium adherence, Biocompatible Materials, Biological materials, biomaterial, Biomaterials, Bone, bone infection, Bone Regeneration, Bone regeneration materials, Clinical strains, Colony Count, Microbial, colony forming unit, Colony forming units, confocal laser microscopy, Confocal laser scanning microscopy, confocal microscopy, Contact angle, Durapatite, hydrophobicity, Microscopy, Confocal, Microscopy, Electron, Scanning, Nano-hydroxyapatite, Nanohydroxyapatite, nanoparticles, nonhuman, porosity, Pseudomonas aeruginosa, Scanning electron microscopy, Sintering, Sintering temperatures, Staphylococcus aureus, Staphylococcus epidermidis, Surface area, Surface feature, surface property, Surface roughness, Surgical procedures, temperature, ultrasound, unclassified drug, wettability, Zeta potential},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861570738\&partnerID=40\&md5=84186d33133ff6c5280db80ec9fcb12d},
	author = {Grenho, L. and Manso, M.C. and Monteiro, F.J. and Ferraz, M.P.}
}
@book {2201,
	title = {Automatic assessment of Leishmania infection indexes on in vitro macrophage cell cultures},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {7325 LNCS},
	year = {2012},
	note = {Conference code: 91199Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-31298-4_51Language of Original Document: EnglishCorrespondence Address: Leal, P.; Universidade Do Porto, Faculdade de Engenharia, Departamento de Engenharia Qu{\'\i}micaPortugalReferences: Castro, H., Sousa, C., Santos, M., Cordeiro-da-Silva, A., Floh{\'e}, L., Tom{\'a}s, A., Complementary antioxidant defence by cytoplasmic and mitochondrial peroxiredoxins in Leishmania infantum (2002) Free Radical Biology and Medicine, 33, pp. 1552-1562;Chen, X., Zhou, X., Wong, S., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Marcuzzo, M., Quelhas, P., Campilho, A., Maria Mendon{\c c}a, A., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Computers in Biology and Medicine, 39 (9); 
Chen, Y., Ladi, E., Herzmark, P., Robey, E., Roysam, B., Automated 5-d analysis of cell migration and interaction in the thymic cortex from time-lapse sequences of 3-d multi-channel multi-photon images (2009) J. Immunol. Methods, 340 (1), pp. 65-80; 
Esteves, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Gradient convergence filters and a phase congruency approach for in vivo cell nuclei detection Machine Vision and Applications, , in press; 
Schmitt, O., Hasse, M., Morphologic multiscale decomposition of connected regions with emphasis on cell clusters (2009) CVIU, 113 (2), pp. 188-201; 
Usaj, M., Torkar, D., Kanduser, M., Miklavcic, D., Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast image (2010) Journal of Microscopy, 241 (3), pp. 303-314; 
Sezgin, M., Sankur, B., Survey over image thresholding techniques and quantitative performance evaluation (2003) Journal of Electronic Imaging, 13 (1), pp. 146-165; 
Lowe, D., Distinctive Image Features from Scale-Invariant Keypoints (2004) International Journal of Computer Vision; 
Lindberg, T., Scale-space theory: A basic tool for analysing structures at different scales (1994) Journal of Applied Statistics, pp. 11-24; 
Yan, P., Zhou, X., Shah, M., Wong, S.T.C., Automatic segmentation of high-throughput RNAi fluorescent cellular images (2008) IEEE Trans. Inf. Technol. Biomed., 12 (1), pp. 109-117; 
CellNote: Plataforma para An?alise Assistida de Imagens, Bruno Afonso Da Cruz Lopes, , Master Thesis, Faculty of Sciences of the University of Porto
},
	month = {2012///},
	pages = {432 - 439},
	address = {Aveiro},
	abstract = {Evaluation of parasite infection indexes on in vitro cell cultures is a practice commonly employed by biomedical researchers to address biological questions or to test the efficacy of novel anti-parasitic compounds. In the particular case of Leishmania infantum, a unicellular parasite that parasitizes macrophages, infection indexes are usually determined either by visual inspection of cells directly under the microscope or by counting digital images using appropriate software. In either case assessment of infection indexes is time consuming, thus motivating the creation of automatic image analysis approaches that allow large scale studies of Leishmania-infected macrophage cultures. We propose a fully automated method for automatic evaluation of parasite infection indexes through the segmentation of individual macrophages nucleus and cytoplasm, as well as the segmentation and co-localization of the parasites in the image. To perform such analysis with robustness and increased performance we propose the use of local image filters tuned to the specific size of the objects to detect, in conjunction with image segmentation approaches. The objects size estimation is then improved through a learning feedback loop. Cytoplasm is detected by seeded watershed segmentation. Our approach obtains, for 86 images from 4 experiments, an average parasite infection index evaluation error of 2.3\%. {\textcopyright} 2012 Springer-Verlag.},
	keywords = {Automated methods, Automatic assessment, Automatic evaluation, Automatic image analysis, Cell culture, Cell nuclei detection, cell nucleus, Colocalization, Digital image, Enzyme inhibition, Feed-back loop, Image analysis, Image filters, Image segmentation, In-vitro, Leishmania, Leishmania infantum, Macrophage cells, Macrophage culture, Macrophages, microscopy image segmentation, Microscopy images, Parasite infection, Parasite-, Size estimation, Visual inspection, Watershed segmentation},
	isbn = {03029743 (ISSN); 9783642312977 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864131020\&partnerID=40\&md5=71523f4ff6de3e6ecc717c2fce28284a},
	author = {Leal, P. and Ferro, L. and Marques, M. and Rom{\~a}o, S. and Cruz, T. and Tom{\'a}, A.M. and Castro, H. and Quelhas, P.}
}
@book {2197,
	title = {Automatic lane detection in chromatography images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {7325 LNCS},
	year = {2012},
	note = {Conference code: 91199Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-31298-4_22Language of Original Document: EnglishCorrespondence Address: Moreira, B.M.; Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: moreira@fe.up.ptReferences: Fried, B., Sherma, J., (1999) Thin-Layer Chromatography, , Marcel Dekker, New York;Rodrigues, L.G., Ferraz, M.J., Rodrigues, D., Pais-Vieira, M., Lima, D., Brady, R.O., S{\'a}-Miranda, M.C., Neurophysiological behavioral and morphological abnormalities in the Fabry knockout mice (2009) Neurobiology of Disease, 33 (1), pp. 48-56; 
Elstein, D., Altarescu, G., Beck, M., (2010) Fabry Disease, , Springer, Heidelberg; 
Houck, M.M., Siegel, J.A., (2010) Fundamentals of Forensic Science, , Elsevier, Oxford; 
Machado, A.M.C., Campos, M.F.M., Siqueira, A.M., De Carvalho, O.S.F., An iterative algorithm for segmenting lanes in gel electrophoresis images (1997) Proc. X Brazilian Symposium on Computer Graphics and Image Processing, pp. 140-146. , October; 
Elder, J.K., Southern, E.M., Computer-aided analysis of one dimensional restriction fragment gels (1987) Nucleic Acid and Protein Sequence Analysis - A Practical Aproach, pp. 165-172. , Bishop, M.J., Rawlings, C.J. (eds.) IRL Press, Oxford; 
Bajla, I., Holl{\"a}nder, I., Fluch, S., Burg, K., Koll{\'a}r, M., An alternative method for electrophoretic gel image analysis in the GelMaster software (2005) Computer Methods and Programs in Biomedicine, 77, pp. 209-231; 
Lin, C., Ching, Y., Yang, Y., An Automatic Method to Compare the Lanes in Gel Electrophoresis (GE) Images (2007) IEEE Transaction on Information Technology in Biomedicine, 11 (2), pp. 179-189; 
Akbari, A., Fritz, A., Jackobsen, K.S., Automatic lane detection and separation in one dimensional gel images using continuous wavelet transform (2010) The Royal Society of Chemistry, Analytical Methods, 2, pp. 1360-1371; 
Sousa, A.V., Aguiar, R.L., Mendon{\c c}a, A.M., Campilho, A.C., Automatic Lane and Band Detection in Images of Thin Layer Chromatography (2004) LNCS, 3212, pp. 158-165. , Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. Springer, Heidelberg; 
Sotaquira, M., On the use of distance maps in the analysis of 1D DNA gel images (2009) ICDIP 2009: Proceedings of the International Conference on Digital Image Processing, pp. 172-176. , IEEE Computer Society; 
Barrantes, P., Alvarado, P., Lane Detection on Gel Electrophoresis Images using Active Shape Models (2011) Proc. of the Conference on Technologies for Sustainable Development, TSD 2011, pp. 43-46. , February; 
Mendon{\c c}a, A.M., Sousa, A.V., S{\'a}-Miranda, M.C., Campilho, A.C., Automatic segmentation of chromatographic images for region of interest delineation (2011) Proc. SPIE, 7962, pp. 79623B1-79623B7; 
Chau, F., Liang, Y., Gao, J., Shao, X., (2004) Chemometrics - from Basics to Wavelet Transform, , John Wiley \& Sons, New Jersey; 
Press, W.H., Flannery, B.P., Teukolsky, S.A., Vetterling, W.T., (1992) Numerical Recipes in C: The Art of Scientific Computing, , Syndicate of the University of Cambridge; 
Soille, P., Jones, C.D., Smith, A.B., Roberts, E.F., (2004) Morphological Image Analysis - Principles and Applications, , Springer; 
Visa, S., Ralescu, A., Learning Imbalanced and Overlapping Classes using Fuzzy Sets Workshop on Learning from Imbalanced Datasets II, ICML (2003)
},
	month = {2012///},
	pages = {180 - 187},
	address = {Aveiro},
	abstract = {This paper proposes a method for automating the detection of lanes in Thin-Layer Chromatography images. Our approach includes a preprocessing step to detect the image region of interest, followed by background estimation and removal. This image is then projected onto the horizontal direction to integrate the information into a one-dimensional profile. A smoothing filter is applied to this profile and the outcome is the input of the lane detection process, which is performed in three phases. The first one aims at obtaining an initial set of candidate lanes that are further validated or removed in the second phase. The last phase is a refinement step that allows the inclusion of lanes that are not clearly distinguishable in the profile and that were not included in the initial set. The method was evaluated in 66 chromatography images and achieved values of recall, precision and F                        β -measure of 97.0\%, 99.4\% and 98.2\%, respectively. {\textcopyright} 2012 Springer-Verlag.
},
	keywords = {Background estimation, Bio-medical image processing, Biomedical image processing, Chromatography, Chromatography images, Computer aided diagnosis, Image analysis, Image regions, Lane detection, Pre-processing step, Refinement step, Second phase, Smoothing filters, Three phasis},
	isbn = {03029743 (ISSN); 9783642312977 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864120489\&partnerID=40\&md5=1e51865766ec865c2127c5b672f97b8f},
	author = {Moreira, B.M. and Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2198,
	title = {Automatic localization of the optic disc in retinal images based on the entropy of vascular directions},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {7325 LNCS},
	year = {2012},
	note = {Conference code: 91199Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-31298-4_50Language of Original Document: EnglishCorrespondence Address: Mendon{\c c}a, A.M.; Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: amendon@fe.up.ptReferences: Jelinek, H., Cree, M., (2010) Automated Image Detection of Retinal Pathology, , CRC Press, Taylor \& Francis Group, Boca Raton;Couper, D.J., Klein, R., Hubbard, L.D., Wong, T.Y., Sorlie, P., Cooper, L.S., Brothers, R.J., Nieto, F.J., Reliability of Retinal Photography in the Assessment of Retinal Microvascular Characteristics: The Atherosclerosis Risk in Communities Study (2002) American Journal of Ophthalmology, 133 (1), pp. 78-88; 
Sinthanayothin, C., Boyce, J.F., Cook, H.L., Williamson, T.H., Automated localization of the optic disc, fovea, and retinal blood vessels from digital colour fundus images (1999) Br. J. Ophthalmol., 83, pp. 902-910; 
Aquino, A., Geg{\'u}ndez-Arias, M.E., Marin, D., Detecting the Optic Disc Boundary in Digital Fundus Images Using Morphological, Edge Detection and Feature Extraction Techniques (2010) IEEE Trans. Medical Imaging, 29 (11), pp. 1860-1869; 
Aquino, A., Geg{\'u}ndez-Arias, M.E., Marin, D., Automated Optic Disc Detection in Retinal Images of Patients with Diabetic Retinopathy and Risk of Macular Edema (2012) Int. J. of Biological and Life Sciences, 8 (2), pp. 87-92; 
Lalonde, M., Beaulieu, M., Gagnon, L., Fast and Robust Optic Disc Detection Using Pyramidal Decomposition and Hausdorff-based Template Matching (2001) IEEE Trans. Medical Imaging, 20 (11), pp. 1193-1200; 
Osareh, A., Mirmehdi, M., Thomas, B., Markham, R., Classification and Localisation of Diabetic-Related Eye Disease (2002) LNCS, 2353, pp. 502-516. , Heyden, A., Sparr, G., Nielsen, M., Johansen, P. (eds.) ECCV 2002. Springer, Heidelberg; 
Lowell, J., Hunter, A., Steel, D., Basu, A., Ryder, R., Fletcher, E., Kennedy, L., Optic Nerve head Segmentation (2004) IEEE Trans. Medical Imaging, 23 (2), pp. 256-264; 
Abdel-Ghafar, R.A., Morris, T., Progress towards automated detection and characterization of the optic disc in glaucoma and diabetic retinopathy (2007) Medical Informatics and the Internet in Medicine, 32 (1), pp. 19-25; 
Hoover, A., Glodbaum, M., Locating the Optic Nerve in a Retinal Image Using the Fuzzy Convergence of the Blood Vessels (2003) IEEE Trans. Medical Imaging, 22 (8), pp. 951-958; 
Foracchia, M., Grisan, E., Ruggeri, A., Detection of Optic Disc in Retinal Images by Means of a Geometrical Model of Vessel Structure (2004) IEEE Trans. Medical Imaging, 23 (10), pp. 1189-1195; 
Haar, F., (2005) Automatic Localization of the Optic Disc in Digital Colour Images of the Human Retina, , Msc Thesis, University of Utrecht; 
Youssif, A., Ghalwash, A.Z., Ghoneim, A., Optic Disc Detection from Normalized Digital Fundus Images by Means of a Vessels{\textquoteright} Direction Matched Filter (2008) IEEE Trans. Medical Imaging, 27 (1), pp. 11-18; 
Niemeijer, M., Abr{\`a}moff, M.D., Van Ginneken, B., Fast detection of the optic disc and fovea in color fundus photographs (2009) Medical Image Analysis, 13, pp. 859-870; 
Xu, X., Gravin, M.K., Abr{\`a}moff, M.D., Reinhardt, J.M., Simultaneous Automatic Detection of the Optic Disc and Fovea on Fundus Photographs (2011) Proc. SPIE Medical Imaging 2011: Image Processing, 7962, pp. 7962T. , Dawant, B.M., Haynor, D.R. (eds.) SPIE; 
http://www.isi.uu.nl/Research/Databases/DRIVEhttp://www.ces.clemson.edu/~ahoover/stare, The STARE projectMendon{\c c}a, A., Campilho, A., Segmentation of Retinal Blood Vessels by Combining the Detection of Centerlines and Morphological Reconstruction (2006) IEEE Trans. Medical Imaging, 25 (9), pp. 1200-1213; 
Chaudhuri, S., Chattererjee, S., Katz, N., Glodbaum, M., Detection of blood Vessels in Retinal Images using Two-Dimensional Matched Filters (1989) IEEE Trans. Medical Imaging, 28 (3), pp. 263-269
},
	month = {2012///},
	pages = {424 - 431},
	address = {Aveiro},
	abstract = {This paper proposes an automatic method for estimating the location of the optic disc in color images of the retina. The proposed methodology is founded in a new concept, the entropy of vascular directions, which proved to be a reliable measure for assessing the convergence of vessels around an image point. To improve the robustness of the method, the search for the maximum value of entropy is restricted to image areas with high intensity. This new method was evaluated in two publicly available databases, containing both normal and pathological images, and was able to obtain a valid location for the optic disc in 115 out of the 121 images of the two datasets. {\textcopyright} 2012 Springer-Verlag.},
	keywords = {Automatic localization, Automatic method, Color images, Data sets, entropy, High intensity, Image analysis, Image points, Maximum values, Ophthalmology, optic disc, Pathological images, Retinal image, Retinal image analysis, vessel segmentation},
	isbn = {03029743 (ISSN); 9783642312977 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864128693\&partnerID=40\&md5=faf6f5351e81f8c595247728be684d95},
	author = {Mendon{\c c}a, A.M. and Cardoso, F. and Sousa, A.V. and Campilho, A.}
}
@booklet {2190,
	title = {Bioengineered surfaces to improve the blood compatibility of biomaterials through direct thrombin inactivation},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.07.020Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre 823, 415email: cmartins@ineb.up.pt},
	month = {2012///},
	abstract = {Thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. This work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. Boophilin, a direct thrombin inhibitor from the cattle tick Rhipicephalus microplus, was produced as a recombinant protein in Pichia pastoris. Boophilin was biotinylated and immobilized on biotin-terminated self-assembled monolayers (SAM) via neutravidin. In order to maintain its proteinase inhibitory capacity after surface immobilization, boophilin was biotinylated after the formation of a boophilin-thrombin complex to minimize the biotinylation of the residues involved in thrombin-boophilin interaction. The extent of boophilin biotinylation was determined using matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. Boophilin immobilization and thrombin adsorption were quantified using quartz crystal microbalance with dissipation. Thrombin competitive adsorption from human serum was assessed using                        125I-thrombin. Thrombin inhibition and plasma clotting time were determined using spectrophotometric techniques. Boophilin-coated SAM were able to promote thrombin adsorption in a selective way, inhibiting most of its activity and delaying plasma coagulation in comparison with boophilin-free surfaces, demonstrating boophilin{\textquoteright}s potential to improve the hemocompatibility of biomaterials used in the production of blood-contacting devices. {\textcopyright} 2012 Acta Materialia Inc.
},
	keywords = {Coagulation, Hemocompatibility, Protein adsorption, Surface functionalization, Thrombin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865246462\&partnerID=40\&md5=278b2e7d1427a6032eba9ecbd01c3872},
	author = {Freitas, S.C. and Cereija, T.B. and Figueiredo, A.C. and Os{\'o}rio, H. and Pereira, P.J.B. and Barbosa, M.A. and Martins, M.C.L.}
}
@booklet {2176,
	title = {Brain delivery of camptothecin by means of solid lipid nanoparticles: Formulation design, in vitro and in vivo studies},
	howpublished = {International Journal of PharmaceuticsInt. J. Pharm.},
	volume = {439},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in PressCODEN: IJPHDdoi: 10.1016/j.ijpharm.2012.09.054Language of Original Document: EnglishCorrespondence Address: Martins, S.; Laboratory of Pharmaceutical Technology/Centre of Research in Pharmaceutical Sciences (LTF/CICF), Faemail: susana.martins@ff.up.pt},
	month = {2012///},
	pages = {49 - 62},
	abstract = {For the purpose of brain delivery upon intravenous injection, formulations of camptothecin-loaded solid lipid nanoparticles (SLN), prepared by hot high pressure homogenisation, were designed. Incorporation of camptothecin in the hydrophobic and acidic environment of SLN matrix was chosen to stabilise the lactone ring, which is essential for its antitumour activity, and for avoiding premature loss of drug on the way to target camptothecin to the brain.A multivariate approach was used to assess the influence of the qualitative and quantitative composition on the physicochemical properties of camptothecin-loaded SLN in comparison to plain SLN. Mean particle sizes of <=200. nm, homogenous size distributions and high encapsulation efficiencies (>90\%) were achieved for the most suitable formulations. In vitro release studies in plasma, showed a prolonged release profile of camptothecin from SLN, confirming the physical stability of the particles under physiological pH. A higher affinity of the SLN to the porcine brain capillary endothelial cells (BCEC) was shown in comparison to macrophages. MTT studies in BCEC revealed a moderate decrease in the cell viability of camptothecin, when incorporated in SLN compared to free camptothecin in solution. In vivo studies in rats showed that fluorescently labelled SLN were detected in the brain after i.v. administration. This study indicates that the camptothecin-loaded SLN are a promising drug brain delivery system worth to explore further for brain tumour therapy. {\textcopyright} 2012 Elsevier B.V.},
	keywords = {Brain capillary endothelial cells (BCEC), Brain delivery, Camptothecin, Multivariate analysis, Solid lipid nanoparticles (SLN), Surfactants},
	isbn = {03785173 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867836553\&partnerID=40\&md5=74d025ee54b5f6569263167e0e4bcfd0},
	author = {Martins, S. and Tho, I. and Reimold, I. and Fricker, G. and Souto, E. and Ferreira, D. and Brandl, M.}
}
@article {2820,
	title = {Dissection and reconstruction of the extracellular matrix - a cardiac regenerative niche},
	journal = {Journal of Tissue Engineering and Regenerative Medicine},
	volume = {6669 LNCS6},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: Scopus},
	month = {2012},
	pages = {110 - 15.P24},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=58726bd7fc58cf587a13af9e3ba0c151},
	author = {Nascimento D. and Silva A. and Oliveira MJ. and Barbosa MA. and Pinto-do-{\'O} P.}
}
@article {2189,
	title = {E-cadherin dysfunction in gastric cancer - Cellular consequences, clinical applications and open questions},
	journal = {FEBS LettersFEBS Lett.},
	volume = {586},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: FEBLAdoi: 10.1016/j.febslet.2012.07.045PubMed ID: 22841718Language of Original Document: EnglishCorrespondence Address: Seruca, R.; IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal; email: rseruca@ipatimup.ptChemicals/CAS: uvomorulin, 112956-45-3; CadherinsReferences: Milne, A.N., Carneiro, F., O{\textquoteright}Morain, C., Offerhaus, G.J., Nature meets nurture: Molecular genetics of gastric cancer (2009) Hum. Genet., 126, pp. 615-628. , 10.1007/s00439-009-0722-x;Lauren, P., The Two Histological Main Types of Gastric Carcinoma: Diffuse and So-Called Intestinal-Type Carcinoma. An Attempt at a Histo-Clinical Classification (1965) Acta. Pathol. Microbiol. Scand., 64, pp. 31-49; 
Hudler, P., Genetic aspects of gastric cancer instability (2012) ScientificWorldJournal, 2012, p. 761909. , 10.1100/2012/761909; 
Oliveira, C., Seruca, R., Carneiro, F., Genetics, pathology, and clinics of familial gastric cancer (2006) Int. J. Surg. Pathol., 14, pp. 21-33; 
Lynch, H.T., Grady, W., Suriano, G., Huntsman, D., Gastric cancer: New genetic developments (2005) J Surg. Oncol., 90, pp. 114-133. , 10.1002/jso.20214 discussion 133; 
Carneiro, F., Amado, M., David, L., Clausen, H., Sobrinho-Simoes, M., Glycosylation features of gastric carcinoma initiation and progression. A review with emphasis on simple mucin-type carbohydrates and histo-blood group antigens of the Lewis system (1994) Eur. J. Cancer Prev., 3 (SUPPL. 2), pp. 39-46; 
Zheng, L., Wang, L., Ajani, J., Xie, K., Molecular basis of gastric cancer development and progression (2004) Gastric Cancer, 7, pp. 61-77. , 10.1007/s10120-004-0277-4; 
Paredes, J., Figueiredo, J., Albergaria, A., Oliveira, P., Carvalho, J., Ribeiro, A.S., Caldeira, J., Oliveira, M.J., Epithelial E- and P-cadherins: Role and clinical significance in cancer (2012) Biochim. Biophys. Acta, 1826, pp. 297-311. , 10.1016/j.bbcan.2012.05.002; 
Figueiredo, C., MacHado, J.C., Pharoah, P., Seruca, R., Sousa, S., Carvalho, R., Capelinha, A.F., Van Doorn, L.J., Helicobacter pylori and interleukin 1 genotyping: An opportunity to identify high-risk individuals for gastric carcinoma (2002) J. Natl. Cancer Inst., 94, pp. 1680-1687; 
Canedo, P., Figueiredo, C., MacHado, J.C., After Helicobacter pylori, genetic susceptibility to gastric carcinoma revisited (2007) Helicobacter, 12 (SUPPL. 2), pp. 45-49. , 10.1111/j.1523-5378.2007.00564.x; 
Peleteiro, B., Lopes, C., Figueiredo, C., Lunet, N., Salt intake and gastric cancer risk according to Helicobacter pylori infection, smoking, tumour site and histological type (2011) Br. J. Cancer, 104, pp. 198-207. , 10.1038/sj.bjc.6605993; 
Price, T.J., Shapiro, J.D., Segelov, E., Karapetis, C.S., Pavlakis, N., Van Cutsem, E., Shah, M.A., Tebbutt, N.C., Management of advanced gastric cancer (2012) Expert Rev. Gastroenterol. Hepatol., 6, pp. 199-208. , 10.1586/egh.11.103 quiz 209; 
Dikken, J.L., Van De Velde, C.J., Coit, D.G., Shah, M.A., Verheij, M., Cats, A., Treatment of resectable gastric cancer (2012) Therap. Adv. Gastroenterol., 5, pp. 49-69. , 10.1177/1756283X11410771; 
Christofori, G., Changing neighbours, changing behaviour: Cell adhesion molecule-mediated signalling during tumour progression (2003) Embo J., 22, pp. 2318-2323. , 10.1093/emboj/cdg228; 
Hanahan, D., Weinberg, R.A., Hallmarks of cancer: The next generation (2011) Curr. Opin. Cell Biol., 144, pp. 646-674. , 10.1016/j.cell.2011.02.013; 
Thiery, J.P., Acloque, H., Huang, R.Y., Nieto, M.A., Epithelial-mesenchymal transitions in development and disease (2009) Curr. Opin. Cell Biol., 139, pp. 871-890. , 10.1016/j.cell.2009.11.007; 
Christofori, G., Snail1 links transcriptional control with epigenetic regulation (2010) Embo J., 29, pp. 1787-1789. , 10.1038/emboj.2010.92; 
David, J.M., Rajasekaran, A.K., Dishonorable Discharge: The Oncogenic Roles of Cleaved E-Cadherin Fragments (2012) Adv. Cancer Res., , 10.1158/0008-5472.CAN-11-3498; 
Van Roy, F., Berx, G., The cell-cell adhesion molecule E-cadherin (2008) Cell Mol. Life Sci., 65, pp. 3756-3788. , 10.1007/s00018-008-8281-1; 
Stemmler, M.P., Cadherins in development and cancer (2008) Mol. Biosyst., 4, pp. 835-850. , 10.1039/b719215k; 
Takeichi, M., Functional correlation between cell adhesive properties and some cell surface proteins (1977) J. Cell. Biol., 75, pp. 464-474; 
Bussemakers, M.J., Van Bokhoven, A., Voller, M., Smit, F.P., Schalken, J.A., The genes for the calcium-dependent cell adhesion molecules P- and E-cadherin are tandemly arranged in the human genome (1994) Biochem. Biophys. Res. Commun., 203, pp. 1291-1294; 
Hubbard, T.J., Aken, B.L., Ayling, S., Ballester, B., Beal, K., Bragin, E., Brent, S., Clarke, L., Ensembl 2009 (2009) Nucleic Acids Res., 37, pp. 690-D697. , 10.1093/nar/gkn828; 
Takeichi, M., Morphogenetic roles of classic cadherins (1995) Curr. Opin. Cell Biol., 7, pp. 619-627; 
Shapiro, L., Fannon, A.M., Kwong, P.D., Thompson, A., Lehmann, M.S., Grubel, G., Legrand, J.F., Hendrickson, W.A., Structural basis of cell-cell adhesion by cadherins (1995) Nature, 374, pp. 327-337. , 10.1038/374327a0; 
Pertz, O., Bozic, D., Koch, A.W., Fauser, C., Brancaccio, A., Engel, J., A new crystal structure, Ca2+ dependence and mutational analysis reveal molecular details of E-cadherin homoassociation (1999) Embo J., 18, pp. 1738-1747. , 10.1093/emboj/18.7.1738; 
Gumbiner, B.M., Regulation of cadherin-mediated adhesion in morphogenesis (2005) Nat. Rev. Mol. Cell Biol., 6, pp. 622-634. , 10.1038/nrm1699; 
Ozawa, M., Ringwald, M., Kemler, R., Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule (1990) Proc. Natl. Acad. Sci. USA, 87, pp. 4246-4250; 
Takeichi, M., Cadherins: A molecular family important in selective cell-cell adhesion (1990) Annu. Rev. Biochem., 59, pp. 237-252. , 10.1146/annurev.bi.59.070190.001321; 
Gumbiner, B.M., McCrea, P.D., Catenins as mediators of the cytoplasmic functions of cadherins (1993) J. Cell Sci. Suppl., 17, pp. 155-158; 
Yap, A.S., Niessen, C.M., Gumbiner, B.M., The juxtamembrane region of the cadherin cytoplasmic tail supports lateral clustering, adhesive strengthening, and interaction with p120ctn (1998) J. Cell. Biol., 141, pp. 779-789; 
Thoreson, M.A., Anastasiadis, P.Z., Daniel, J.M., Ireton, R.C., Wheelock, M.J., Johnson, K.R., Hummingbird, D.K., Reynolds, A.B., Selective uncoupling of p120(ctn) from E-cadherin disrupts strong adhesion (2000) J. Cell. Biol., 148, pp. 189-202; 
Nishimura, T., Takeichi, M., Remodeling of the adherens junctions during morphogenesis (2009) Curr. Top. Dev. Biol., 89, pp. 33-54. , 10.1016/S0070-2153(09)89002-9; 
Hyafil, F., Morello, D., Babinet, C., Jacob, F., A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos (1980) Curr. Opin. Cell Biol., 21, pp. 927-934; 
Takeichi, M., The cadherins: Cell-cell adhesion molecules controlling animal morphogenesis (1988) Development, 102, pp. 639-655; 
Fleming, T.P., Javed, Q., Hay, M., Epithelial differentiation and intercellular junction formation in the mouse early embryo (1992) Development, pp. 105-112; 
Nose, A., Takeichi, M., A novel cadherin cell adhesion molecule: Its expression patterns associated with implantation and organogenesis of mouse embryos (1986) J. Cell Biol., 103, pp. 2649-2658; 
Aplin, J.D., Jones, C.J., Harris, L.K., Adhesion molecules in human trophoblast - A review (2009) I. Villous Trophoblast. Placenta, 30, pp. 293-298. , 10.1016/j.placenta.2008.12.001; 
Larue, L., Ohsugi, M., Hirchenhain, J., Kemler, R., E-cadherin null mutant embryos fail to form a trophectoderm epithelium (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 8263-8267; 
Frebourg, T., Oliveira, C., Hochain, P., Karam, R., Manouvrier, S., Graziadio, C., Vekemans, M., Alexandre, C., Cleft lip/palate and CDH1/E-cadherin mutations in families with hereditary diffuse gastric cancer (2006) J. Med. Genet., 43, pp. 138-142. , 10.1136/jmg.2005.031385; 
Halbleib, J.M., Nelson, W.J., Cadherins in development: Cell adhesion, sorting, and tissue morphogenesis (2006) Genes Dev., 20, pp. 3199-3214. , 10.1101/gad.1486806; 
Cano, A., Perez-Moreno, M.A., Rodrigo, I., Locascio, A., Blanco, M.J., Del Barrio, M.G., Portillo, F., Nieto, M.A., The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression (2000) Nat. Cell Biol., 2, pp. 76-83. , 10.1038/35000025; 
Batlle, E., Sancho, E., Franci, C., Dominguez, D., Monfar, M., Baulida, J., Garcia De Herreros, A., The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells (2000) Nat. Cell Biol., 2, pp. 84-89. , 10.1038/35000034; 
Conacci-Sorrell, M., Simcha, I., Ben-Yedidia, T., Blechman, J., Savagner, P., Ben-Ze{\textquoteright}Ev, A., Autoregulation of E-cadherin expression by cadherin-cadherin interactions: The roles of beta-catenin signaling, Slug, and MAPK (2003) J. Cell. Biol., 163, pp. 847-857. , 10.1083/jcb.200308162; 
Comijn, J., Berx, G., Vermassen, P., Verschueren, K., Van Grunsven, L., Bruyneel, E., Mareel, M., Van Roy, F., The two-handed e box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion (2001) Nat. Rev. Mol. Cell Biol., 7, pp. 1267-1278; 
Peinado, H., Olmeda, D., Cano, A., Snail, Zeb and bHLH factors in tumour progression: An alliance against the epithelial phenotype? (2007) Nat. Rev. Cancer, 7, pp. 415-428. , 10.1038/nrc2131; 
Graff, J.R., Herman, J.G., Lapidus, R.G., Chopra, H., Xu, R., Jarrard, D.F., Isaacs, W.B., Baylin, S.B., E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas (1995) Adv. Cancer Res., 55, pp. 5195-5199; 
Strathdee, G., Epigenetic versus genetic alterations in the inactivation of E-cadherin (2002) Semin. Cancer Biol., 12, pp. 373-379; 
Oliveira, C., Sousa, S., Pinheiro, H., Karam, R., Bordeira-Carrico, R., Senz, J., Kaurah, P., Gusmao, L., Quantification of epigenetic and genetic 2nd hits in CDH1 during hereditary diffuse gastric cancer syndrome progression (2009) Gastroenterology, 136, pp. 2137-2148. , 10.1053/j.gastro.2009.02.065; 
Pinheiro, H., Bordeira-Carrico, R., Seixas, S., Carvalho, J., Senz, J., Oliveira, P., Inacio, P., Huntsman, D., Allele-specific CDH1 downregulation and hereditary diffuse gastric cancer (2010) Hum. Mol. Genet., 19, pp. 943-952. , 10.1093/hmg/ddp537; 
Hosono, S., Gross, I., English, M.A., Hajra, K.M., Fearon, E.R., Licht, J.D., E-cadherin is a WT1 target gene (2000) J. Biol. Chem., 275, pp. 10943-10953; 
Chen, Y.T., Stewart, D.B., Nelson, W.J., Coupling assembly of the E-cadherin/beta-catenin complex to efficient endoplasmic reticulum exit and basal-lateral membrane targeting of E-cadherin in polarized MDCK cells (1999) J. Cell. Biol., 144, pp. 687-699; 
Lickert, H., Bauer, A., Kemler, R., Stappert, J., Casein kinase II phosphorylation of E-cadherin increases E-cadherin/beta-catenin interaction and strengthens cell-cell adhesion (2000) J. Biol. Chem., 275, pp. 5090-5095; 
Bryant, D.M., Stow, J.L., The ins and outs of E-cadherin trafficking (2004) Trends Cell Biol., 14, pp. 427-434. , 10.1016/j.tcb.2004.07.007; 
Lilien, J., Balsamo, J., The regulation of cadherin-mediated adhesion by tyrosine phosphorylation/dephosphorylation of beta-catenin (2005) Curr. Opin. Cell Biol., 17, pp. 459-465. , 10.1016/j.ceb.2005.08.009; 
Huber, A.H., Weis, W.I., The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin (2001) Curr. Opin. Cell Biol., 105, pp. 391-402; 
Zhu, W., Leber, B., Andrews, D.W., Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis (2001) Embo J., 20, pp. 5999-6007. , 10.1093/emboj/20.21.5999; 
Pinho, S.S., Seruca, R., Gartner, F., Yamaguchi, Y., Gu, J., Taniguchi, N., Reis, C.A., Modulation of E-cadherin function and dysfunction by N-glycosylation (2011) Cell Mol. Life Sci., 68, pp. 1011-1020. , 10.1007/s00018-010-0595-0; 
Liwosz, A., Lei, T., Kukuruzinska, M.A., N-glycosylation affects the molecular organization and stability of E-cadherin junctions (2006) J. Biol. Chem., 281, pp. 23138-23149. , 10.1074/jbc.M512621200; 
Palacios, F., Price, L., Schweitzer, J., Collard, J.G., D{\textquoteright}Souza-Schorey, C., An essential role for ARF6-regulated membrane traffic in adherens junction turnover and epithelial cell migration (2001) Embo J., 20, pp. 4973-4986. , 10.1093/emboj/20.17.4973; 
Figueiredo, J., Simoes-Correia, J., Soderberg, O., Suriano, G., Seruca, R., ADP-ribosylation factor 6 mediates E-cadherin recovery by chemical chaperones (2011) PLoS One, 6, p. 23188. , 10.1371/journal.pone.0023188; 
Aparicio, L.A., Valladares, M., Blanco, M., Alonso, G., Figueroa, A., Biological influence of Hakai in cancer: A 10-year review (2012) Cancer Metastasis Rev., 31, pp. 375-386. , 10.1007/s10555-012-9348-x; 
Shen, Y., Hirsch, D.S., Sasiela, C.A., Wu, W.J., Cdc42 regulates E-cadherin ubiquitination and degradation through an epidermal growth factor receptor to Src-mediated pathway (2008) J. Biol. Chem., 283, pp. 5127-5137. , 10.1074/jbc.M703300200; 
Palacios, F., Tushir, J.S., Fujita, Y., D{\textquoteright}Souza-Schorey, C., Lysosomal targeting of E-cadherin: A unique mechanism for the down-regulation of cell-cell adhesion during epithelial to mesenchymal transitions (2005) Nat. Rev. Mol. Cell Biol., 25, pp. 389-402. , 10.1128/MCB.25.1.389-402.2005; 
Davis, M.A., Ireton, R.C., Reynolds, A.B., A core function for p120-catenin in cadherin turnover (2003) J. Cell. Biol., 163, pp. 525-534. , 10.1083/jcb.200307111; 
Berx, G., Van Roy, F., Involvement of members of the cadherin superfamily in cancer (2009) Cold Spring Harb. Perspect Biol., 1, p. 003129. , 10.1101/cshperspect.a003129; 
De Wever, O., Derycke, L., Hendrix, A., De Meerleer, G., Godeau, F., Depypere, H., Bracke, M., Soluble cadherins as cancer biomarkers (2007) Clin. Exp. Metastasis, 24, pp. 685-697. , 10.1007/s10585-007-9104-8; 
Rodriguez, F.J., Lewis-Tuffin, L.J., Anastasiadis, P.Z., E-cadherin{\textquoteright}s dark side: Possible role in tumor progression (2012) Biochim. Biophys. Acta, 1826, pp. 23-31. , 10.1016/j.bbcan.2012.03.002; 
Yilmaz, M., Christofori, G., Mechanisms of motility in metastasizing cells (2010) Mol. Cancer Res., 8, pp. 629-642. , 10.1158/1541-7786.MCR-10-0139; 
Birchmeier, W., Behrens, J., Cadherin expression in carcinomas: Role in the formation of cell junctions and the prevention of invasiveness (1994) Biochim. Biophys. Acta, 1198, pp. 11-26; 
Christofori, G., Semb, H., The role of the cell-adhesion molecule E-cadherin as a tumour-suppressor gene (1999) Trends Biochem. Sci., 24, pp. 73-76; 
Guilford, P., Humar, B., Blair, V., Hereditary diffuse gastric cancer: Translation of CDH1 germline mutations into clinical practice (2010) Gastric Cancer, 13, pp. 1-10. , 10.1007/s10120-009-0531-x; 
Guilford, P., Hopkins, J., Harraway, J., McLeod, M., McLeod, N., Harawira, P., Taite, H., Reeve, A.E., E-cadherin germline mutations in familial gastric cancer (1998) Nature, 392, pp. 402-405. , 10.1038/32918; 
Oliveira, C., Senz, J., Kaurah, P., Pinheiro, H., Sanges, R., Haegert, A., Corso, G., Vogelsang, H., Germline CDH1 deletions in hereditary diffuse gastric cancer families (2009) Hum. Mol. Genet., 18, pp. 1545-1555. , 10.1093/hmg/ddp046; 
Humar, B., Blair, V., Charlton, A., More, H., Martin, I., Guilford, P., E-cadherin deficiency initiates gastric signet-ring cell carcinoma in mice and man (2009) Adv. Cancer Res., 69, pp. 2050-2056. , 10.1158/0008-5472.CAN-08-2457; 
Carneiro, F., Oliveira, C., Seruca, R., Pathology and genetics of familial gastric cancer (2010) Int. J. Surg. Pathol., 18, pp. 33S-36S. , 10.1177/1066896910366463; 
Caldas, C., Carneiro, F., Lynch, H.T., Yokota, J., Wiesner, G.L., Powell, S.M., Lewis, F.R., Jankowski, J.A., Familial gastric cancer: Overview and guidelines for management (1999) J. Med. Genet., 36, pp. 873-880; 
Becker, K.F., Atkinson, M.J., Reich, U., Becker, I., Nekarda, H., Siewert, J.R., Hofler, H., E-cadherin gene mutations provide clues to diffuse type gastric carcinomas (1994) Adv. Cancer Res., 54, pp. 3845-3852; 
Berx, G., Cleton-Jansen, A.M., Strumane, K., De Leeuw, W.J., Nollet, F., Van Roy, F., Cornelisse, C., E-cadherin is inactivated in a majority of invasive human lobular breast cancers by truncation mutations throughout its extracellular domain (1996) Oncogene, 13, pp. 1919-1925; 
MacHado, J.C., Oliveira, C., Carvalho, R., Soares, P., Berx, G., Caldas, C., Seruca, R., Sobrinho-Simoes, M., E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma (2001) Oncogene, 20, pp. 1525-1528. , 10.1038/sj.onc.1204234; 
Corso, G., Pedrazzani, C., Pinheiro, H., Fernandes, E., Marrelli, D., Rinnovati, A., Pascale, V., Roviello, F., E-cadherin genetic screening and clinico-pathologic characteristics of early onset gastric cancer (2011) Eur. J. Cancer, 47, pp. 631-639. , 10.1016/j.ejca.2010.10.011; 
Masciari, S., Larsson, N., Senz, J., Boyd, N., Kaurah, P., Kandel, M.J., Harris, L.N., Miron, P., Germline E-cadherin mutations in familial lobular breast cancer (2007) J. Med. Genet., 44, pp. 726-731. , 10.1136/jmg.2007.051268; 
Schrader, K.A., Masciari, S., Boyd, N., Salamanca, C., Senz, J., Saunders, D.N., Yorida, E., Tung, N., Germline mutations in CDH1 are infrequent in women with early-onset or familial lobular breast cancers (2011) J. Med. Genet., 48, pp. 64-68. , 10.1136/jmg.2010.079814; 
Corso, G., Marrelli, D., Pascale, V., Vindigni, C., Roviello, F., Frequency of CDH1 germline mutations in gastric carcinoma coming from high- and low-risk areas: Metanalysis and systematic review of the literature (2012) Br. J. Cancer, 12, p. 8. , 10.1186/1471-2407-12-8; 
Suriano, G., Oliveira, C., Ferreira, P., MacHado, J.C., Bordin, M.C., De Wever, O., Bruyneel, E.A., Porter, T.R., Identification of CDH1 germline missense mutations associated with functional inactivation of the E-cadherin protein in young gastric cancer probands (2003) Hum. Mol. Genet., 12, pp. 575-582; 
Brooks-Wilson, A.R., Kaurah, P., Suriano, G., Leach, S., Senz, J., Grehan, N., Butterfield, Y.S., Bacani, J., Germline E-cadherin mutations in hereditary diffuse gastric cancer: Assessment of 42 new families and review of genetic screening criteria (2004) J. Med. Genet., 41, pp. 508-517; 
Kaurah, P., MacMillan, A., Boyd, N., Senz, J., De Luca, A., Chun, N., Suriano, G., Gilpin, C., Founder and recurrent CDH1 mutations in families with hereditary diffuse gastric cancer (2007) JAMA, 297, pp. 2360-2372. , 10.1001/jama.297.21.2360; 
Corso, G., Roviello, F., Paredes, J., Pedrazzani, C., Novais, M., Correia, J., Marrelli, D., Oliveira, C., Characterization of the P373L E-cadherin germline missense mutation and implication for clinical management (2007) Eur J Surg Oncol, 33, pp. 1061-1067. , 10.1016/j.ejso.2007.03.001; 
More, H., Humar, B., Weber, W., Ward, R., Christian, A., Lintott, C., Graziano, F., Boman, B., Identification of seven novel germline mutations in the human E-cadherin (CDH1) gene (2007) Hum. Mutat., 28, p. 203. , 10.1002/humu.9473; 
Suriano, G., Oliveira, M.J., Huntsman, D., Mateus, A.R., Ferreira, P., Casares, F., Oliveira, C., Mareel, M., E-cadherin germline missense mutations and cell phenotype: Evidence for the independence of cell invasion on the motile capabilities of the cells (2003) Hum. Mol. Genet., 12, pp. 3007-3016. , 10.1093/hmg/ddg316; 
Suriano, G., Seixas, S., Rocha, J., Seruca, R., A model to infer the pathogenic significance of CDH1 germline missense variants (2006) Acta. Pathol. Microbiol. Scand., 84, pp. 1023-1031. , 10.1007/s00109-006-0091-z; 
Simoes-Correia, J., Figueiredo, J., Lopes, R., Stricher, F., Oliveira, C., Serrano, L., Seruca, R., E-cadherin destabilization accounts for the pathogenicity of missense mutations in hereditary diffuse gastric cancer (2012) PLoS One, 7, p. 33783. , 10.1371/journal.pone.0033783; 
Figueiredo J, S., The importance of E-cadherin binding partners to evaluate the pathogenicity of E-cadherin missense mutations associated to HDGC Eur. J. Hum Genetics, , in press; 
Becker, K.F., Kremmer, E., Eulitz, M., Becker, I., Handschuh, G., Schuhmacher, C., Muller, W., Hirohashi, S., Analysis of E-cadherin in diffuse-type gastric cancer using a mutation-specific monoclonal antibody (1999) Annu. Rev. Biochem., 155, pp. 1803-1809. , 10.1016/S0002-9440(10)65497-1; 
Grady, W.M., Willis, J., Guilford, P.J., Dunbier, A.K., Toro, T.T., Lynch, H., Wiesner, G., Park, J.G., Methylation of the CDH1 promoter as the second genetic hit in hereditary diffuse gastric cancer (2000) Nat. Genet., 26, pp. 16-17. , 10.1038/79120; 
Oliveira, C., De Bruin, J., Nabais, S., Ligtenberg, M., Moutinho, C., Nagengast, F.M., Seruca, R., Carneiro, F., Intragenic deletion of CDH1 as the inactivating mechanism of the wild-type allele in an HDGC tumour (2004) Oncogene, 23, pp. 2236-2240. , 10.1038/sj.onc.1207335; 
Barber, M., Murrell, A., Ito, Y., Maia, A.T., Hyland, S., Oliveira, C., Save, V., Grehan, N., Mechanisms and sequelae of E-cadherin silencing in hereditary diffuse gastric cancer (2008) Annu. Rev. Biochem., 216, pp. 295-306. , 10.1002/path.2426; 
Yoshiura, K., Kanai, Y., Ochiai, A., Shimoyama, Y., Sugimura, T., Hirohashi, S., Silencing of the E-cadherin invasion-suppressor gene by CpG methylation in human carcinomas (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 7416-7419; 
Karayiannakis, A.J., Syrigos, K.N., Chatzigianni, E., Papanikolaou, S., Karatzas, G., E-cadherin expression as a differentiation marker in gastric cancer (1998) Hepatogastroenterology, 45, pp. 2437-2442; 
He, L., Hannon, G.J., MicroRNAs: Small RNAs with a big role in gene regulation (2004) Nat. Rev. Genet., 5, pp. 522-531. , 10.1038/nrg1379; 
Korpal, M., Lee, E.S., Hu, G., Kang, Y., The miR-200 family inhibits epithelial-mesenchymal transition and cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2 (2008) J. Biol. Chem., 283, pp. 14910-14914. , 10.1074/jbc.C800074200; 
Park, S.M., Gaur, A.B., Lengyel, E., Peter, M.E., The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2 (2008) Genes Dev., 22, pp. 894-907. , 10.1101/gad.1640608; 
Carvalho, J., Van Grieken, N.C., Pereira, P.M., Sousa, S., Tijssen, M., Buffart, T.E., Diosdado, B., Meijer, G., Lack of microRNA-101 causes E-cadherin functional deregulation through EZH2 up-regulation in intestinal gastric cancer (2012) Annu. Rev. Biochem., , 10.1002/path.4032; 
Simoes-Correia, J., Figueiredo, J., Oliveira, C., Van Hengel, J., Seruca, R., Van Roy, F., Suriano, G., Endoplasmic reticulum quality control: A new mechanism of E-cadherin regulation and its implication in cancer (2008) Hum. Mol. Genet., 17, pp. 3566-3576. , 10.1093/hmg/ddn249; 
Pinho, S.S., Osorio, H., Nita-Lazar, M., Gomes, J., Lopes, C., Gartner, F., Reis, C.A., Role of E-cadherin N-glycosylation profile in a mammary tumor model (2009) Biochem. Biophys. Res. Commun., 379, pp. 1091-1096. , 10.1016/j.bbrc.2009.01.024; 
Pinho, S.S., Reis, C.A., Paredes, J., Magalhaes, A.M., Ferreira, A.C., Figueiredo, J., Xiaogang, W., Seruca, R., The role of N-acetylglucosaminyltransferase III and v in the post-transcriptional modifications of E-cadherin (2009) Hum. Mol. Genet., 18, pp. 2599-2608. , 10.1093/hmg/ddp194; 
Ferreira, P., Oliveira, M.J., Beraldi, E., Mateus, A.R., Nakajima, T., Gleave, M., Yokota, J., Seruca, R., Loss of functional E-cadherin renders cells more resistant to the apoptotic agent taxol in vitro (2005) Exp. Cell Res., 310, pp. 99-104. , 10.1016/j.yexcr.2005.07.010; 
Ferreira, A.C., Suriano, G., Mendes, N., Gomes, B., Wen, X., Carneiro, F., Seruca, R., MacHado, J.C., E-cadherin impairment increases cell survival through Notch-dependent upregulation of Bcl-2 (2012) Hum. Mol. Genet., 21, pp. 334-343. , 10.1093/hmg/ddr469; 
Carvalho, R., Kayademir, T., Soares, P., Canedo, P., Sousa, S., Oliveira, C., Leistenschneider, P., Blin, N., Loss of heterozygosity and promoter methylation, but not mutation, may underlie loss of TFF1 in gastric carcinoma (2002) Lab. Invest., 82, pp. 1319-1326; 
Cavallaro, U., Dejana, E., Adhesion molecule signalling: Not always a sticky business (2011) Nat. Rev. Mol. Cell Biol., 12, pp. 189-197. , 10.1038/nrm3068; 
Nelson, W.J., Nusse, R., Convergence of Wnt, beta-catenin, and cadherin pathways (2004) Science, 303, pp. 1483-1487. , 10.1126/science.1094291; 
Suriano, G., Mulholland, D., De Wever, O., Ferreira, P., Mateus, A.R., Bruyneel, E., Nelson, C.C., Huntsman, D., The intracellular E-cadherin germline mutation V832 M lacks the ability to mediate cell-cell adhesion and to suppress invasion (2003) Oncogene, 22, pp. 5716-5719. , 10.1038/sj.onc.1206672; 
Pan, Y., Bi, F., Liu, N., Xue, Y., Yao, X., Zheng, Y., Fan, D., Expression of seven main Rho family members in gastric carcinoma (2004) Biochem. Biophys. Res. Commun., 315, pp. 686-691. , 10.1016/j.bbrc.2004.01.108; 
Mateus, A.R., Seruca, R., MacHado, J.C., Keller, G., Oliveira, M.J., Suriano, G., Luber, B., EGFR regulates RhoA-GTP dependent cell motility in E-cadherin mutant cells (2007) Hum. Mol. Genet., 16, pp. 1639-1647. , 10.1093/hmg/ddm113; 
Bremm, A., Walch, A., Fuchs, M., Mages, J., Duyster, J., Keller, G., Hermannstadter, C., Langer, R., Enhanced activation of epidermal growth factor receptor caused by tumor-derived E-cadherin mutations (2008) Adv. Cancer Res., 68, pp. 707-714. , 10.1158/0008-5472.CAN-07-1588; 
Mateus, A.R., Simoes-Correia, J., Figueiredo, J., Heindl, S., Alves, C.C., Suriano, G., Luber, B., Seruca, R., E-cadherin mutations and cell motility: A genotype-phenotype correlation (2009) Exp. Cell Res., 315, pp. 1393-1402. , 10.1016/j.yexcr.2009.02.020; 
Zhou, Y., Li, G., Wu, J., Zhang, Z., Wu, Z., Fan, P., Hao, T., Zhang, F., Clinicopathological significance of E-cadherin, VEGF, and MMPs in gastric cancer (2010) Tumour Biol., 31, pp. 549-558. , 10.1007/s13277-010-0068-y; 
Zhang, J.F., Zhang, Y.P., Hao, F.Y., Zhang, C.X., Li, Y.J., Ji, X.R., DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma (2005) World J. Gastroenterol., 11, pp. 5592-5600; 
Fuchs, M., Hermannstadter, C., Specht, K., Knyazev, P., Ullrich, A., Rosivatz, E., Busch, R., Luber, B., Effect of tumor-associated mutant E-cadherin variants with defects in exons 8 or 9 on matrix metalloproteinase 3 (2005) J. Cell Physiol., 202, pp. 805-813. , 10.1002/jcp. 20192; 
Noe, V., Fingleton, B., Jacobs, K., Crawford, H.C., Vermeulen, S., Steelant, W., Bruyneel, E., Mareel, M., Release of an invasion promoter E-cadherin fragment by matrilysin and stromelysin-1 (2001) J. Cell Sci. Suppl., 114, pp. 111-118; 
Uemura, N., Okamoto, S., Yamamoto, S., Matsumura, N., Yamaguchi, S., Yamakido, M., Taniyama, K., Schlemper, R.J., Helicobacter pylori infection and the development of gastric cancer (2001) N. Engl. J. Med., 345, pp. 784-789. , 10.1056/NEJMoa001999; 
Chan, A.O., Lam, S.K., Wong, B.C., Wong, W.M., Yuen, M.F., Yeung, Y.H., Hui, W.M., Kwong, Y.L., Promoter methylation of E-cadherin gene in gastric mucosa associated with Helicobacter pylori infection and in gastric cancer (2003) Gut, 52, pp. 502-506; 
Huang, F.Y., Chan, A.O., Rashid, A., Wong, D.K., Cho, C.H., Yuen, M.F., Helicobacter pylori induces promoter methylation of E-cadherin via interleukin-1beta activation of nitric oxide production in gastric cancer cells (2012) Adv. Cancer Res., , 10.1002/cncr.27519; 
Schirrmeister, W., Gnad, T., Wex, T., Higashiyama, S., Wolke, C., Naumann, M., Lendeckel, U., Ectodomain shedding of E-cadherin and c-Met is induced by Helicobacter pylori infection (2009) Exp. Cell Res., 315, pp. 3500-3508. , 10.1016/j.yexcr.2009.07.029; 
Hoy, B., Lower, M., Weydig, C., Carra, G., Tegtmeyer, N., Geppert, T., Schroder, P., Schneider, G., Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E-cadherin to disrupt intercellular adhesion (2010) EMBO Rep., 11, pp. 798-804. , 10.1038/embor.2010.114; 
Conlin, V.S., Curtis, S.B., Zhao, Y., Moore, E.D., Smith, V.C., Meloche, R.M., Finlay, B.B., Buchan, A.M., Helicobacter pylori infection targets adherens junction regulatory proteins and results in increased rates of migration in human gastric epithelial cells (2004) Infect. Immun., 72, pp. 5181-5192. , 10.1128/IAI.72.9.5181-5192.2004; 
Suzuki, M., Mimuro, H., Suzuki, T., Park, M., Yamamoto, T., Sasakawa, C., Interaction of CagA with Crk plays an important role in Helicobacter pylori-induced loss of gastric epithelial cell adhesion (2005) J. Exp. Med., 202, pp. 1235-1247. , 10.1084/jem.20051027; 
Bebb, J.R., Leach, L., Zaitoun, A., Hand, N., Letley, D.P., Thomas, R., Atherton, J.C., Effects of Helicobacter pylori on the cadherin-catenin complex (2006) J. Clin. Pathol., 59, pp. 1261-1266. , 10.1136/jcp. 2006.036772; 
Oliveira, M.J., Costa, A.M., Costa, A.C., Ferreira, R.M., Sampaio, P., MacHado, J.C., Seruca, R., Figueiredo, C., CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell-invasive phenotype (2009) J. Infect. Dis., 200, pp. 745-755. , 10.1086/604727; 
Oliveira, M.J., Costa, A.C., Costa, A.M., Henriques, L., Suriano, G., Atherton, J.C., MacHado, J.C., Mareel, M., Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system-dependent manner (2006) J. Biol. Chem., 281, pp. 34888-34896. , 10.1074/jbc.M607067200; 
Mimata, A., Fukamachi, H., Eishi, Y., Yuasa, Y., Loss of E-cadherin in mouse gastric epithelial cells induces signet ring-like cells, a possible precursor lesion of diffuse gastric cancer (2011) Cancer Sci., 102, pp. 942-950. , 10.1111/j.1349-7006.2011.01890.x; 
Shimada, Y., Niho, S., Ishii, G., Hishida, T., Yoshida, J., Nishimura, M., Yoh, K., Ohe, Y., Clinical features of unresectable high-grade lung neuroendocrine carcinoma diagnosed using biopsy specimens (2012) Lung Cancer, 75, pp. 368-373. , 10.1016/j.lungcan.2011.08.012; 
Kuwabara, Y., Yamada, T., Yamazaki, K., Du, W.L., Banno, K., Aoki, D., Sakamoto, M., Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis (2008) Cancer Sci., 99, pp. 1933-1939. , 10.1111/j.1349-7006.2008.00946.x; 
Pereira, P.S., Teixeira, A., Pinho, S., Ferreira, P., Fernandes, J., Oliveira, C., Seruca, R., Casares, F., E-cadherin missense mutations, associated with hereditary diffuse gastric cancer (HDGC) syndrome, display distinct invasive behaviors and genetic interactions with the Wnt and Notch pathways in Drosophila epithelia (2006) Hum. Mol. Genet., 15, pp. 1704-1712. , 10.1093/hmg/ddl093; 
Caldeira, J., Pereira, P.S., Suriano, G., Casares, F., Using fruitflies to help understand the molecular mechanisms of human hereditary diffuse gastric cancer (2009) Int. J. Dev. Biol., 53, pp. 1557-1561. , 10.1387/ijdb.072277jc; 
Frixen, U.H., Behrens, J., Sachs, M., Eberle, G., Voss, B., Warda, A., Lochner, D., Birchmeier, W., E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells (1991) J. Cell. Biol., 113, pp. 173-185; 
Wong, A.S., Gumbiner, B.M., Adhesion-independent mechanism for suppression of tumor cell invasion by E-cadherin (2003) J. Cell. Biol., 161, pp. 1191-1203. , 10.1083/jcb.200212033; 
Yanagisawa, M., Anastasiadis, P.Z., P120 catenin is essential for mesenchymal cadherin-mediated regulation of cell motility and invasiveness (2006) J. Cell. Biol., 174, pp. 1087-1096. , 10.1083/jcb.200605022; 
Dunbier, A., Guilford, P., Hereditary diffuse gastric cancer (2001) Adv. Cancer Res., 83, pp. 55-65; 
Friedl, P., Hegerfeldt, Y., Tusch, M., Collective cell migration in morphogenesis and cancer (2004) Int. J. Dev. Biol., 48, pp. 441-449. , 10.1387/ijdb.041821; 
Pedrazzani, C., Caruso, S., Corso, G., Marrelli, D., Neri, A., Berardi, A., Roviello, F., Influence of age on soluble E-cadherin serum levels prevents its utility as a disease marker in gastric cancer patients (2008) Scand. J. Gastroenterol., 43, pp. 765-766. , 10.1080/00365520802037370; 
Hua, H., Li, M., Luo, T., Yin, Y., Jiang, Y., Matrix metalloproteinases in tumorigenesis: An evolving paradigm (2011) Cell Mol. Life Sci., 68, pp. 3853-3868. , 10.1007/s00018-011-0763-x; 
Cisco, R.M., Norton, J.A., Hereditary diffuse gastric cancer: Surgery, surveillance and unanswered questions (2008) Future Oncol., 4, pp. 553-559. , 10.2217/14796694.4.4.553; 
Fitzgerald, R.C., Hardwick, R., Huntsman, D., Carneiro, F., Guilford, P., Blair, V., Chung, D.C., Van Krieken, J.H., Hereditary diffuse gastric cancer: Updated consensus guidelines for clinical management and directions for future research (2010) J. Med. Genet., 47, pp. 436-444. , 10.1136/jmg.2009.074237; 
Gregory, D.J., Mikhaylova, L., Fedulov, A.V., Selective DNA demethylation by fusion of TDG with a sequence-specific DNA-binding domain (2012) Epigenetics, 7, pp. 344-349. , 10.4161/epi.19509; 
Leong, K.G., Niessen, K., Kulic, I., Raouf, A., Eaves, C., Pollet, I., Karsan, A., Jagged1-mediated Notch activation induces epithelial-to-mesenchymal transition through Slug-induced repression of E-cadherin (2007) J. Exp. Med., 204, pp. 2935-2948. , 10.1084/jem.20071082; 
Cavallaro, U., Christofori, G., Cell adhesion and signalling by cadherins and Ig-CAMs in cancer (2004) Nat. Rev. Cancer, 4, pp. 118-132. , 10.1038/nrc1276
},
	month = {2012///},
	pages = {2981 - 2989},
	abstract = {E-cadherin plays a major role in cell-cell adhesion and inactivating germline mutations in its encoding gene predispose to hereditary diffuse gastric cancer. Evidence indicates that aside from its recognized role in early tumourigenesis, E-cadherin is also pivotal for tumour progression, including invasion and metastization. Herein, we discuss E-cadherin alterations found in a cancer context, associated cellular effects and signalling pathways, and we raise new key questions that will impact in the management of GC patients and families. {\textcopyright} 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.},
	keywords = {Adhesion, Cadherins, cancer growth, cancer invasion, cancer patient, cancer susceptibility, cell adhesion, complex formation, conference paper, E-cadherin, embryo development, endocytosis, epigenetics, epithelial mesenchymal transition, Gastric cancer, gene mutation, gene silencing, genetic susceptibility, Germ-Line Mutation, HDGC, Helicobacter infection, Helicobacter Infections, Helicobacter pylori, histone deacetylase inhibitor, human, Humans, Invasion, metastasis, molecular pathology, nonhuman, priority journal, protein analysis, protein depletion, protein expression, protein function, protein structure, regulatory mechanism, signal transduction, Signalling, stomach cancer, stomach carcinogenesis, Stomach Neoplasms, Tumour, uvomorulin},
	isbn = {00145793 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864922992\&partnerID=40\&md5=455ae054dc6c8f95dc9e81d77fec1ad9},
	author = {Carneiro, P. and Fernandes, M.S. and Figueiredo, J. and Caldeira, J. and Carvalho, J. and Pinheiro, H. and Leite, M. and Melo, S. and Oliveira, P. and Sim{\~o}es-Correia, J. and Oliveira, M.J. and Carneiro, F. and Figueiredo, C. and Paredes, J. and Oliveira, C. and Seruca, R.}
}
@article {2187,
	title = {The effect of adsorbed fibronectin and osteopontin on macrophage adhesion and morphology on hydrophilic and hydrophobic model surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {8},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2012.06.010Language of Original Document: EnglishCorrespondence Address: Barbosa, M.A.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mbarbosa@ineb.up.ptChemicals/CAS: fibronectin, 86088-83-7; gold, 7440-57-5; osteopontin, 106441-73-0References: Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110;Horbett, T.A., Brash, J.L., Proteins at interfaces: Current issues and future prospects (1987) Proteins at Interfaces, pp. 1-33; 
Dee, K.C., Puleo, D.A., Bizios, R., Protein-surface interactions (2003) An Introduction to Tissue-biomaterial Interactions, pp. 37-37. , New York: John Wiley \& Sons Inc; 
Wilson, C.J., Clegg, R.E., Leavesley, D.I., Pearcy, M.J., Mediation of biomaterial-cell interactions by adsorbed proteins: A review (2005) Tissue Eng, 11, pp. 1-18; 
Allen, L.T., Surface-induced changes in protein adsorption and implications for cellular phenotypic responses to surface interaction (2006) Biomaterials, 27, pp. 3096-3108; 
Kou, P.M., Babensee, J.E., Macrophage and dendritic cell phenotypic diversity in the context of biomaterials (2011) J Biomed Mater Res, 96, pp. 239-260; 
Defife, K.M., Colton, E., Nakayama, Y., Matsuda, T., Anderson, J.M., Spatial regulation and surface chemistry control of monocyte/macrophage adhesion and foreign body giant cell formation by photochemically micropatterned surfaces (1999) J Biomed Mater Res, 45, pp. 148-154; 
Collier, T.O., Anderson, J.M., Kikuchi, A., Okano, T., Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly(n-isopropylacrylamide) temperature-responsive surfaces (2002) J Biomed Mater Res, 59, pp. 136-143; 
Jenney, C.R., Anderson, J.M., Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formation in vitro (1999) J Biomed Mater Res, 44, pp. 206-216; 
Jenney, C.R., Anderson, J.M., Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation (1999) J Biomed Mater Res, 46, pp. 11-21; 
Collier, T.O., Anderson, J.M., Protein and surface effects on monocyte and macrophage adhesion, maturation, and survival (2002) J Biomed Mater Res, 60, pp. 487-496; 
Kao, W.J., Hubbell, J.A., Anderson, J.M., Protein-mediated macrophage adhesion and activation on biomaterials: A model for modulating cell behavior (1999) J Mater Sci Mater Med, 10, pp. 601-605; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (2000) J Biomed Mater Res, 49, pp. 435-447; 
Jenney, C.R., Anderson, J.M., Adsorbed IgG: A potent adhesive substrate for human macrophages (2000) J Biomed Mater Res, 50, pp. 281-290; 
Kao, W.J., Lee, D., Schense, J.C., Hubbell, J.A., Fibronectin modulates macrophage adhesion and FBGC formation: The role of RGD, PHSRN, and PRRARV domains (2001) J Biomed Mater Res, 55, pp. 79-88; 
Kao, W.J., Evaluation of protein-modulated macrophage behavior on biomaterials: Designing biomimetic materials for cellular engineering (1999) Biomaterials, 20, pp. 2213-2221; 
Giamblanco, N., Yaseen, M., Zhavnerko, G., Lu, J.R., Marletta, G., Fibronectin conformation switch induced by coadsorption with human serum albumin (2010) Langmuir, 27, pp. 312-319; 
Anderson, J.M., The extracellular matrix and biomaterials (1994) Implantation Biology: The Host Response and Biomedical Devices, pp. 113-130; 
Pankov, R., Yamada, K.M., Fibronectin at a glance (2002) J Cell Sci, 115, pp. 3861-3863; 
Pierschbacher, M.D., Ruoslahti, E., Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule (1984) Nature, 309, pp. 30-33; 
Lund, S., Giachelli, C., Scatena, M., The role of osteopontin in inflammatory processes (2009) J Cell Commun Signal, 3, pp. 311-322; 
Denhardt, D.T., Giachelli, C.M., Rittling, S.R., Role of osteopontin in cellular signaling and toxicant injury (2001) Annu Rev Pharmacol Toxicol, 41, pp. 723-749; 
Denhardt, D.T., Noda, M., O{\textquoteright}Regan, A.W., Pavlin, D., Berman, J.S., Osteopontin as a means to cope with environmental insults: Regulation of inflammation, tissue remodeling, and cell survival (2001) J Clin Invest, 107, pp. 1055-1061; 
Denhardt, D., Guo, X., Osteopontin: A protein with diverse functions (1993) FASEB J, 7, pp. 1475-1482; 
Kazanecki, C.C., Uzwiak, D.J., Denhardt, D.T., Control of osteopontin signaling and function by post-translational phosphorylation and protein folding (2007) J Cell Biochem, 102, pp. 912-924; 
Pedraza, C.E., Nikolcheva, L.G., Kaartinen, M.T., Barralet, J.E., McKee, M.D., Osteopontin functions as an opsonin and facilitates phagocytosis by macrophages of hydroxyapatite-coated microspheres: Implications for bone wound healing (2008) Bone, 43, pp. 708-716; 
Giachelli, C.M., Lombardi, D., Johnson, R.J., Murry, C.E., Almeida, M., Evidence for a role of osteopontin in macrophage infiltration in response to pathological stimuli in vivo (1998) Am J Pathol, 152, pp. 353-358; 
Tsai, A.T., Rice, J., Scatena, M., Liaw, L., Ratner, B.D., Giachelli, C.M., The role of osteopontin in foreign body giant cell formation (2005) Biomaterials, 26, pp. 5835-5843; 
Jensen, T., Osteopontin functionalization of hydroxyapatite nanoparticles in a PDLLA matrix promotes bone formation (2011) J Biomed Mater Res, 99, pp. 94-101; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol, 25, pp. 55-78; 
Barrias, C.C., Martins, M.C.L., Almeida-Porada, G., Barbosa, M.A., Granja, P.L., The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers (2009) Biomaterials, 30, pp. 307-316; 
Martins, M.C., Fonseca, C., Barbosa, M.A., Ratner, B.D., Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry (2003) Biomaterials, 24, pp. 3697-3706; 
Sivaraman, B., Fears, K.P., Latour, R.A., Investigation of the effects of surface chemistry and solution concentration on the conformation of adsorbed proteins using an improved circular dichroism method (2009) Langmuir, 25, pp. 3050-3056; 
MacIel, J., Martins, M.C.L., Barbosa, M.A., The stability of self-assembled monolayers with time and under biological conditions (2010) J Biomed Mater Res, 94, pp. 833-843; 
Martins, M.C., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) J Biomed Mater Res, 67, pp. 158-171; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferrira, J., Almeida, B., Soasa, J.B., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Martins, M.C.L., Curtin, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res, 88, pp. 162-173; 
McNally, A.K., Anderson, J.M., Complement C3 participation in monocyte adhesion to different surfaces (1994) Proc Natl Acad Sci USA, 91, pp. 10119-10123; 
Martins, M.C., Sousa, S., Antunes, J., Barbosa, M., Protein adsorption characterization (2012) Nanotechnology in Regenerative Medicine, pp. 141-161; 
Barrias, C.C., Martins, C.L., Miranda, C.S., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: Effect of surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
Goncalves, R., Martins, M.C.L., Oliveira, M.J., Almeida-Porada, G., Barbosa, M.A., Bioactivity of immobilized EGF on self-assembled monolayers: Optimization of the immobilization process (2010) J Biomed Mater Res, 94, pp. 576-585; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, , Amersham Life Science Little Chalfont, UK: Amersham, International; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res, 89, pp. 642-653; 
Burmeister, J.S., Vrany, J.D., Reichert, W.M., Truskey, G.A., Effect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA/EMA copolymers (1996) J Biomed Mater Res, 30, pp. 13-22; 
Shen, M., Horbett, T.A., The effects of surface chemistry and adsorbed proteins on monocyte/macrophage adhesion to chemically modified polystyrene surfaces (2001) J Biomed Mater Res, 57, pp. 336-345; 
Brodbeck, W.G., Colton, E., Anderson, J.M., Effects of adsorbed heat labile serum proteins and fibrinogen on adhesion and apoptosis of monocytes/macrophages on biomaterials (2003) J Mater Sci Mater Med, 14, pp. 671-675; 
Curtis, A.S., Forrester, J.V., The competitive effects of serum proteins on cell adhesion (1984) J Cell Sci, 71, pp. 17-35; 
Roach, P., Farrar, D., Perry, C.C., Interpretation of protein adsorption: Surface-induced conformational changes (2005) J Am Chem Soc, 127, pp. 8168-8173; 
Harder, P., Grunze, M., Dahint, R., Whitesides, G.M., Laibinis, P.E., Molecular conformation in oligo(ethylene glycol)-terminated self-assembled monolayers on gold and silver surfaces determines their ability to resist protein adsorption (1998) J Phys Chem B, 102, pp. 426-436; 
Stein, M.J., Weidner, T., McCrea, K., Castner, D.G., Ratner, B.D., Hydration of sulphobetaine and tetra(ethylene glycol)-terminated self-assembled monolayers studied by sum frequency generation vibrational spectroscopy (2009) J Phys Chem B, 113, pp. 11550-11556; 
Latour, R.A., Biomaterials: Protein-surface interactions (2008) The Encyclopedia of Biomaterials and Biomedical Engineering, pp. 270-284; 
Michael, K.E., Vernekar, V.N., Keselowsky, B.G., Meredith, J.C., Latour, R.A., Garc{\'\i}a, A.J., Adsorption-induced conformational changes in fibronectin due to interactions with well-defined surface chemistries (2003) Langmuir, 19, pp. 8033-8040; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res, 66, pp. 247-259; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J.A.J., Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding (2004) Biomaterials, 25, pp. 5947-5954; 
Wittmer, C.R., Phelps, J.A., Saltzman, W.M., Van Tassel, P.R., Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies (2007) Biomaterials, 28, pp. 851-860; 
McNally, A.K., Anderson, J.M., 1 and β2 integrins mediate adhesion during macrophage fusion and multinucleated foreign body giant cell formation (2002) Am J Pathol, 160, pp. 621-630; 
Liu, L., Chen, S., Giachelli, C.M., Ratner, B.D., Jiang, S., Controlling osteopontin orientation on surfaces to modulate endothelial cell adhesion (2005) J Biomed Mater Res, 74, pp. 23-31; 
Malmstrom, J., Christensen, B., Lovmand, J., Sorensen, E.S., Duch, M., Sutherland, D.S., Osteopontin presentation affects cell adhesion - Influence of underlying surface chemistry and nanopatterning of osteopontin (2010) J Biomed Mater Res, 95, pp. 518-530
},
	month = {2012///},
	pages = {3669 - 3677},
	abstract = {Macrophages play a crucial role in the host response to biomaterials. Here we investigated the effect of adsorbed fibronectin (FN) and osteopontin (OPN), two important proteins for tissue repair, on macrophage adhesion and morphology. Since cell-biomaterial interactions are modulated via proteins adsorbed onto biomaterial surfaces, FN and OPN were adsorbed on model self-assembled monolayers (SAMs) of alkanethiols on gold with different functional terminal groups (CH                        3, OH and tetra(ethylene-glycol)). The initial interaction of inflammatory cells with a biomaterial is crucial for the ensuing phases of an inflammatory reaction. For this reason short-term cultures of primary human macrophages were performed. To account for the competitive adsorption of other proteins serum was added to the culture medium and the effect compared with serum-free medium cultures. In the presence of serum hydrophilic surfaces increased macrophage adhesion. In particular, FN induced a higher cell density, while OPN tended to decrease it. In serum-free medium cell adhesion was greater on hydrophobic surfaces, except for OPN-coated SAMs. Importantly, FN no longer enhanced macrophage adhesion, while OPN maintained its inhibitory effect. Cell polarization studies indicated that macrophage morphology variations induced by surface chemistry are overcome by pre-adsorbed OPN. Taken together our results show that in the presence of serum macrophage adhesion is promoted by FN hydrophilic surfaces, but impaired on OPN-coated surfaces. The effects of inhibited macrophage adhesion on macrophage fusion, and its relevance to the initial stages of the inflammatory response to biomaterials are discussed. {\textcopyright} 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
},
	keywords = {alkane, article, biomaterial, Biomaterials, cell adhesion, cell density, cell interaction, cell structure, controlled study, culture medium, cytoskeleton, Fibronectin, functional group, gold, human, human cell, hydrophilicity, hydrophobicity, inflammatory cell, macrophage, Macrophages, nanomaterial, Osteopontin, priority journal, self assembled monolayer, Self-assembled monolayers, Tetra, thiol, tissue repair},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865455542\&partnerID=40\&md5=9d34fb780e9ce53b424143b0f3eefb97},
	author = {Maciel, J. and Oliveira, M.I. and Gonalves, R.M. and Barbosa, M.A.}
}
@booklet {2186,
	title = {Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.09.011Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 4150email: cmartins@ineb.up.pt},
	month = {2012///},
	abstract = {Helicobacter pylori infection has been associated with several gastric diseases. This bacterium colonizes the gastric mucosa of half of the world{\textquoteright}s population, and available treatments are unsuccessful in practically one in every five patients. Mucoadhesive polymers, such as chitosan, are being investigated as gastric drug delivery systems. However, since chitosan is also known for its antimicrobial properties, this work aims to evaluate H. pylori interactions with chitosan under simulated gastric environments, namely using various pHs (2.6, 4 and 6), pepsin and urea. To enable the visualization of adherent bacteria, ultrathin chitosan films were produced by spin-coating on gold/glass surfaces, cross-linked with genipin and characterized by Fourier transform infrared reflection absorption spectroscopy, ellipsometry and electrokinetic analysis. Films with homogeneous thickness of 11.7 {\textpm} 0.6 nm were produced, and were stable and protonated at all the pHs used. Furthermore, they adsorbed pepsin in all these pHs, in contrast to urea, of which a small adsorption was only observed at pH 6. H. pylori binding to chitosan was higher at pH 2.6 although most of adherent bacteria were dead. The presence of pepsin decreased bacterial adhesion, but increased its viability while in a more stressed morphology (coccoid form). The presence of urea did not affect the amount, morphology or viability of chitosan-adherent bacteria. In suspension, the decrease in pH changed H. pylori zeta potential from negative to positive. Moreover, bacteria were only culturable when incubated in pH 6 with and without urea (without pepsin). This work demonstrates that chitosan has the capacity to bind and kill H. pylori in a range of pHs independently of urea. This opens new perspectives for the application of chitosan-based materials to the elimination of H. pylori gastric colonization, though pepsin might appear to be an obstacle. {\textcopyright} 2012 Acta Materialia Inc.},
	keywords = {Bacterial adhesion, Biomaterials, chitosan, Helicobacter pylori, Thin films},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867507239\&partnerID=40\&md5=defd903a9833b81e1d4e9e377550ce07},
	author = {Nogueira, F. and Gon{\c c}alves, I.C. and Martins, M.C.L.}
}
@article {2183,
	title = {The effect of octadecyl chain immobilization on the hemocompatibility of poly (2-hydroxyethyl methacrylate)},
	journal = {BiomaterialsBiomaterials},
	volume = {33},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2012.07.007Language of Original Document: EnglishCorrespondence Address: Sperling, C.; Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany; email: sperling@ipfdd.deChemicals/CAS: isocyanate, 71000-82-3, 75-13-8; polymacon, 25053-81-0, 25249-16-5, 98932-78-6References: Gorbet, M.B., Sefton, M.V., Biomaterial-associated thrombosis: roles of coagulation factors, complement, platelets and leukocytes (2004) Biomaterials, 25, pp. 5681-5703;Cao, L., Sukavaneshvar, S., Ratner, B.D., Horbett, T.A., Glow discharge plasma treatment of polyethylene tubing with tetraglyme results in ultralow fibrinogen adsorption and greatly reduced platelet adhesion (2006) J Biomed Mater Res A, 79, pp. 788-803; 
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Kasori, Y., Shiroya, T., Blood compatible aspects of poly(2-methoxyethylacrylate) (PMEA)-relationship between protein adsorption and platelet adhesion on PMEA surface (2000) Biomaterials, 21, pp. 1471-1481; 
Martins, C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolayers (2003) J Biomed Mater Res, 67 A, pp. 158-171; 
Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials science - an introduction to materials in medicine, pp. 237-46. , Academic Press, San Diego, CA, USA; 
Ratner, B.D., The catastrophe revisited: blood compatibility in the 21st century (2007) Biomaterials, 28, pp. 5144-5147; 
Ji, J., Barbosa, M.A., Feng, L.X., Shen, J.C., A novel urethane containing copolymer as a surface modification additive for blood contact materials (2002) J Mater Sci Mater Med, 13, pp. 677-684; 
Fujisawa, N., Bertram, C.D., Woodard, J.C., Poole-Warren, L.A., Schindhelm, K., Fluid dynamics of a textured blood-contacting surface (2001) J Biomech Eng, 123, pp. 97-105; 
Keogh, J.R., Eaton, J.W., Albumin binding surfaces for biomaterials (1994) J Lab Clin Med, 124, pp. 537-545; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., Alkyl substituted polymers with enhanced albumin affinity (1981) Trans Am Soc Artif Intern Organs, 27, pp. 499-503; 
Sweryda-Krawiec, B., Devaraj, H., Jacob, G., Hickman, J.J., A new interpretation of serum albumin surface passivation (2004) Langmuir, 20, pp. 2054-2056; 
Amiji, M., Park, K., Surface modification of polymeric biomaterials with poly(ethylene oxide), albumin, and heparin for reduced thrombogenicity (1993) J Biomater Sci Polym Ed, 4, pp. 217-234; 
Roach, P., Parker, T., Gadegaard, N., Alexander, M.R., Surface strategies for control of neuronal cell adhesion: a review (2010) Surf Sci Rep, 65, pp. 145-173; 
Weng, Y.J., Hou, R.X., Li, G.C., Wang, J., Huang, N., Liu, H.Q., Immobilization of bovine serum albumin on TiO 2 film via chemisorption of H 3PO 4 interface and effects on platelets adhesion (2008) Appl Surf Sci, 254, pp. 2712-2719; 
Kang, I.-K., Kwon, B.K., Lee, J.H., Lee, H.B., Immobilization of proteins on poly(methyl methacrylate) films (1993) Biomaterials, 14, pp. 787-792; 
Graham, D.E., Phillips, M.C., Proteins at liquid interfaces. Kinetics of adsorption and surface denaturation (1979) J Colloid Interface Sci, 70, pp. 403-414; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption and clotting time of pHEMA hydrogels modified with C18 ligands to adsorb albumin selectively and reversibly (2009) Biomaterials, 30, pp. 5541-5551; 
Streller, U., Sperling, C., Hubner, J., Hanke, R., Werner, C., Design and evaluation of novel blood incubation systems for in vitro hemocompatibility assessment of planar solid surfaces (2003) J Biomed Mater Res B, 66, pp. 379-390; 
Sperling, C., Maitz, M.F., Talkenberger, S., Gouzy, M.F., Groth, T., Werner, C., In vitro blood reactivity to hydroxylated and non-hydroxylated polymer surfaces (2007) Biomaterials, 28, pp. 3617-3625; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-terminated self-assembled monolayers (2005) Biomaterials, 26, pp. 3891-3899; 
Goertzen, W.K., Kessler, M.R., Dynamic mechanical analysis of carbon/epoxy composites for structural pipeline repair (2007) Compos Part B Eng, 38, pp. 1-9; 
Ebnesajjad, S., Surface and material characterization techniques (2006) Surface treatment of materials for adhesion bonding, pp. 43-73. , William Andrew Publishing; 
Meakin, J.R., Hukins, D.W.L., Aspden, R.M., Imrie, C.T., Rheological properties of poly(2-hydroxyethyl methacrylate) (pHEMA) as a function of water content and deformation frequency (2003) J Mater Sci Mater Med, 14, pp. 783-787; 
Kemal, E., Adesanya, K.O., Deb, S., Phosphate based 2-hydroxyethyl methacrylate hydrogels for biomedical applications (2011) J Mater Chem, 21, pp. 2237-2245; 
(2002) Biological evaluation of medical devices - part 4: selection of tests for interaction with blood, , ISO 1993-4:2002; 
Kern, W.P.D.A., Cleaning solutions based on hydrogen peroxide for use in silicon semiconductor technology (1970) RCA Rev, 31, pp. 187-206; 
Cras, J.J., Rowe-Taitt, C.A., Nivens, D.A., Ligler, F.S., Comparison of chemical cleaning methods of glass in preparation for silanization (1999) Biosens Bioelectron, 14, pp. 683-688; 
Sperling, C., Fischer, M., Maitz, M.F., Werner, C., Blood coagulation on biomaterials requires the combination of distinct activation processes (2009) Biomaterials, 30, pp. 4447-4456; 
Park, J.G., Ye, Q., Topp, E.M., Lee, C.H., Kostoryz, E.L., Misra, A., Dynamic mechanical analysis and esterase degradation of dentin adhesives containing a branched methacrylate (2009) J Biomed Mater Res B, 91, pp. 61-70; 
Hu, W.J., Eaton, J.W., Ugarova, T.P., Tang, L., Molecular basis of biomaterial-mediated foreign body reactions (2001) Blood, 98, pp. 1231-1238; 
Franc, R.D., Dresbach, H., Thelen, H., Sieberth, H.-G., Glutardialdehyde induced fluorescence technique (GIFT): a new method for the imaging of platelet adhesion on biomaterials (2000) J Biomed Mater Res, 52, pp. 374-381; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Downer, A., Morris, N., Feast, W.J., Stickler, D., Polymer surface properties and their effect on the adhesion of Proteus mirabilis (2003) Proc Inst Mech Eng H, 217, pp. 279-289; 
Crespin, M., Moreau, N., Masereel, B., Feron, O., Gallez, B., Vander Borght, T., Surface properties and cell adhesion onto allylamine-plasma and amine-plasma coated glass coverslips (2011) J Mater Sci Mater Med, 22, pp. 671-682; 
Maboudian, R., Howe, R.T., Critical review: adhesion in surface micromechanical structures (1997) J Vac Sci Technol B, 15, pp. 1-20; 
G{\"u}le{\c c}, H.A., SarIoglu, K., Mutlu, M., Modification of food contacting surfaces by plasma polymerisation technique. Part I: determination of hydrophilicity, hydrophobicity and surface free energy by contact angle method (2006) J Food Eng, 75, pp. 187-195; 
Cowling, M.J., Hodgkiess, T., Parr, A.C., Smith, M.J., Marrs, S.J., An alternative approach to antifouling based on analogues of natural processes (2000) Sci Total Environ, 258, pp. 129-137; 
Kwok, C.S., Mourad, P.D., Crum, L.A., Ratner, B.D., Surface modification of polymers with self-assembled molecular structures: multitechnique surface characterization (2000) Biomacromolecules, 1, pp. 139-148; 
Gobel, R.J., Janatova, J., Googe, J.M., Apple, D.J., Activation of complement in human serum by some synthetic polymers used for intraocular lenses (1987) Biomaterials, 8, pp. 285-288; 
Payne, M.S., Horbett, T.A., Complement activation by hydroxyethyl-methacrylate-ethylmethacrylate copolymers (1987) J Biomed Mater Res, 21, pp. 843-859; 
Vasilev, K., Mierczynska, A., Hook, A.L., Chan, J., Voelcker, N.H., Short, R.D., Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient Biomaterials:392-7Ikeda, K., Nagasawa, K., Horiuchi, T., Tsuru, T., Nishizaka, H., Niho, Y., C5a induces tissue factor activity on endothelial cells (1997) Thromb Haemost, 77, pp. 394-398; 
Tedesco, F., Pausa, M., Nardon, E., Introna, M., Mantovani, A., Dobrina, A., The cytolytically inactive terminal complement complex activates endothelial cells to express adhesion molecules and tissue factor procoagulant activity (1997) J Exp Med, 185, pp. 1619-1627; 
Fischer, M., Sperling, C., Tengvall, P., Werner, C., The ability of surface characteristics of materials to trigger leukocyte tissue factor expression (2009) Biomaterials, 31, pp. 2498-2507; 
Iuliano, L., Colavita, A.R., Leo, R., Pratic{\`o}, D., Violi, F., Oxygen free radicals and platelet activation (1997) Free Radic Biol Med, 22, pp. 999-1006; 
Khodr, B., Khalil, Z., Modulation of inflammation by reactive oxygen species: implications for aging and tissue repair (2001) Free Radic Biol Med, 30, pp. 1-8; 
Mao, X-Y., Cheng, X., Wang, X., Wu, S-J., Free-radical-scavenging and anti-inflammatory effect of yak milk casein before and after enzymatic hydrolysis Food Chem;126:484-90Pawlak, K., Naumnik, B., Brzosko, S., Pawlak, D., Myliwiec, M., Oxidative stress - a link between endothelial injury, coagulation activation, and atherosclerosis in haemodialysis patients (2004) Am J Nephrol, 24, pp. 154-161; 
Nilsson, B., Ekdahl, K.N., Mollnes, T.E., Lambris, J.D., The role of complement in biomaterial-induced inflammation (2007) Mol Immunol, 44, pp. 82-94; 
Laudes, I.J., Chu, J.C., Sikranth, S., Huber-Lang, M., Guo, R.-F., Riedemann, N., Anti-C5a ameliorates coagulation/fibrinolytic protein changes in a rat model of sepsis (2002) Am J Pathol, 160, pp. 1867-1875
},
	month = {2012///},
	pages = {7677 - 7685},
	abstract = {Albumin-scavenging surfaces decorated with n-alkyl chains represent an established strategy for blood-contacting applications. To evaluate this concept, a set of poly (2-hydroxyethyl methacrylate) (pHEMA) films modified with different amounts of octadecyl isocyanate (C18) was investigated in an in vitro hemocompatibility assay using freshly drawn human whole blood. In addition, the hydrogel materials were thoroughly characterized with respect to changes in wettability and elasticity, which accompanied the gradual chemical modification of pHEMA. An increase of the surface C18 content induced enhanced hydrophobicity and stiffness. Immobilization of C18 chains was found to substantially reduce the coagulation activation and the complement activation by the pHEMA films. Platelet adhesion and degranulation (PF4 release) were similar on the modified and the unmodified pHEMA. Platelet adhesion to pHEMA hydrogels was lower than the polytetrafluoroethylene reference. We conclude that the immobilization of octadecyl chains improved the hemocompatibility of pHEMA materials under conditions that might be encountered in low shear blood flow. {\textcopyright} 2012 Elsevier Ltd.},
	keywords = {Adhesion, Albumin, article, Biological materials, Biomaterials, Blood, Blood flow, Chemical modification, Coagulation, controlled study, Degranulation, Elasticity, Haemocompatibility, Hemocompatibility, human, hydrogel, in vitro study, In-vitro, isocyanate, Octadecyl, octadecyl isocyanate, PHEMA, Platelet adhesion, Platelets, Poly(2-hydroxyethyl methacrylate), PolyHEMA, polymacon, priority journal, Protein adsorption, thrombocyte adhesion, unclassified drug, wettability, Whole blood},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865188580\&partnerID=40\&md5=81a8750115106c3d6e9fa2de862d9f70},
	author = {Fischer, M. and Baptista, C.P. and Gon{\c c}alves, I.C. and Ratner, B.D. and Sperling, C. and Werner, C. and Martins, C.L. and Barbosa, M.A.}
}
@article {2177,
	title = {Epithelial E- and P-cadherins: Role and clinical significance in cancer},
	journal = {Biochimica et Biophysica Acta - Reviews on CancerBiochim. Biophys. Acta Rev. Cancer},
	volume = {1826},
	year = {2012},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: BBACEdoi: 10.1016/j.bbcan.2012.05.002Language of Original Document: EnglishCorrespondence Address: Seruca, R.; IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal; email: rseruca@ipatimup.ptChemicals/CAS: uvomorulin, 112956-45-3Tradenames: pf 03732010References: Cavallaro, U., Christofori, G., Cell adhesion and signalling by cadherins and Ig-CAMs in cancer (2004) Nat. Rev. Cancer, 4, pp. 118-132;Gerrow, K., El-Husseini, A., Cell adhesion molecules at the synapse (2006) Front. Biosci., 11, pp. 2400-2419; 
Nollet, F., Kools, P., van Roy, F., Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members (2000) J. Mol. Biol., 299, pp. 551-572; 
Takeichi, M., Cadherins in cancer: implications for invasion and metastasis (1993) Curr. Opin. Cell Biol., 5, pp. 806-811; 
Pignatelli, M., Bodmer, W.F., Genetics and biochemistry of collagen binding-triggered glandular differentiation in a human colon carcinoma cell line (1988) Proc. Natl. Acad. Sci. U. S. A., 85, pp. 5561-5565; 
Takeichi, M., Morphogenetic roles of classic cadherins (1995) Curr. Opin. Cell Biol., 7, pp. 619-627; 
Shapiro, L., Fannon, A.M., Kwong, P.D., Thompson, A., Lehmann, M.S., Grubel, G., Legrand, J.F., Hendrickson, W.A., Structural basis of cell-cell adhesion by cadherins (1995) Nature, 374, pp. 327-337; 
Ozawa, M., Ringwald, M., Kemler, R., Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule (1990) Proc. Natl. Acad. Sci. U. S. A., 87, pp. 4246-4250; 
Blaschuk, O.W., Sullivan, R., David, S., Pouliot, Y., Identification of a cadherin cell adhesion recognition sequence (1990) Dev. Biol., 139, pp. 227-229; 
Ozawa, M., Baribault, H., Kemler, R., The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species (1989) EMBO J., 8, pp. 1711-1717; 
Gumbiner, B.M., McCrea, P.D., Catenins as mediators of the cytoplasmic functions of cadherins (1993) J. Cell Sci. Suppl., 17, pp. 155-158; 
Yap, A.S., Niessen, C.M., Gumbiner, B.M., The juxtamembrane region of the cadherin cytoplasmic tail supports lateral clustering, adhesive strengthening, and interaction with p120ctn (1998) J. Cell Biol., 141, pp. 779-789; 
Van Aken, E., De Wever, O., Correia da Rocha, A.S., Mareel, M., Defective E-cadherin/catenin complexes in human cancer (2001) Virchows Arch., 439, pp. 725-751; 
Mareel, M., Oliveira, M.J., Madani, I., Cancer invasion and metastasis: interacting ecosystems (2009) Virchows Arch., 454, pp. 599-622; 
Makrilia, N., Kollias, A., Manolopoulos, L., Syrigos, K., Cell adhesion molecules: role and clinical significance in cancer (2009) Cancer Invest., 27, pp. 1023-1037; 
Bussemakers, M.J., van Bokhoven, A., Voller, M., Smit, F.P., Schalken, J.A., The genes for the calcium-dependent cell adhesion molecules P- and E-cadherin are tandemly arranged in the human genome (1994) Biochem. Biophys. Res. Commun., 203, pp. 1291-1294; 
Berx, G., Staes, K., van Hengel, J., Molemans, F., Bussemakers, M.J., van Bokhoven, A., van Roy, F., Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1) (1995) Genomics, 26, pp. 281-289; 
Jarrard, D.F., Paul, R., van Bokhoven, A., Nguyen, S.H., Bova, G.S., Wheelock, M.J., Johnson, K.R., Isaacs, W.B., P-cadherin is a basal cell-specific epithelial marker that is not expressed in prostate cancer (1997) Clin. Cancer Res., 3, pp. 2121-2128; 
Hubbard, T.J., Aken, B.L., Ayling, S., Ballester, B., Beal, K., Bragin, E., Brent, S., Flicek, P., Ensembl 2009 (2009) Nucleic Acids Res., 37, pp. D690-D697; 
Faraldo, M.L., Cano, A., The 5{\textquoteright} flanking sequences of the mouse P-cadherin gene. Homologies to 5{\textquoteright} sequences of the E-cadherin gene and identification of a first 215 base-pair intron (1993) J. Mol. Biol., 231, pp. 935-941; 
van Roy, F., Berx, G., The cell-cell adhesion molecule E-cadherin (2008) Cell. Mol. Life Sci., 65, pp. 3756-3788; 
Stemmler, M.P., Cadherins in development and cancer (2008) Mol. Biosyst., 4, pp. 835-850; 
Paredes, J., Albergaria, A., Oliveira, J.T., Jeronimo, C., Milanezi, F., Schmitt, F.C., P-cadherin overexpression is an indicator of clinical outcome in invasive breast carcinomas and is associated with CDH3 promoter hypomethylation (2005) Clin. Cancer Res., 11, pp. 5869-5877; 
Sakharkar, K.R., Chow, V.T., PPD-proteome profile database (2004) In Silico Biol., 4, pp. 219-223; 
Thomas, D.J., Rosenbloom, K.R., Clawson, H., Hinrichs, A.S., Trumbower, H., Raney, B.J., Karolchik, D., Kent, W.J., The ENCODE Project at UC Santa Cruz (2007) Nucleic Acids Res., 35, pp. D663-D667; 
Oliveira, P., Sanges, R., Huntsman, D., Stupka, E., Oliveira, C., Characterization of the intronic portion of cadherin superfamily members, common cancer orchestrators (2012) Eur. J. Hum. Genet.; 
Hosono, S., Gross, I., English, M.A., Hajra, K.M., Fearon, E.R., Licht, J.D., E-cadherin is a WT1 target gene (2000) J. Biol. Chem., 275, pp. 10943-10953; 
Liu, Y.N., Lee, W.W., Wang, C.Y., Chao, T.H., Chen, Y., Chen, J.H., Regulatory mechanisms controlling human E-cadherin gene expression (2005) Oncogene, 24, pp. 8277-8290; 
Behrens, J., Lowrick, O., Klein-Hitpass, L., Birchmeier, W., The E-cadherin promoter: functional analysis of a G.C-rich region and an epithelial cell-specific palindromic regulatory element (1991) Proc. Natl. Acad. Sci. U. S. A., 88, pp. 11495-11499; 
Cano, A., Perez-Moreno, M.A., Rodrigo, I., Locascio, A., Blanco, M.J., del Barrio, M.G., Portillo, F., Nieto, M.A., The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression (2000) Nat. Cell Biol., 2, pp. 76-83; 
Hajra, K.M., Chen, D.Y., Fearon, E.R., The SLUG zinc-finger protein represses E-cadherin in breast cancer (2002) Cancer Res., 62, pp. 1613-1618; 
Perez-Moreno, M.A., Locascio, A., Rodrigo, I., Dhondt, G., Portillo, F., Nieto, M.A., Cano, A., A new role for E12/E47 in the repression of E-cadherin expression and epithelial-mesenchymal transitions (2001) J. Biol. Chem., 276, pp. 27424-27431; 
Comijn, J., Berx, G., Vermassen, P., Verschueren, K., van Grunsven, L., Bruyneel, E., Mareel, M., van Roy, F., The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion (2001) Mol. Cell, 7, pp. 1267-1278; 
Eger, A., Aigner, K., Sonderegger, S., Dampier, B., Oehler, S., Schreiber, M., Berx, G., Foisner, R., DeltaEF1 is a transcriptional repressor of E-cadherin and regulates epithelial plasticity in breast cancer cells (2005) Oncogene, 24, pp. 2375-2385; 
Peinado, H., Olmeda, D., Cano, A., Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype? (2007) Nat. Rev. Cancer, 7, pp. 415-428; 
Shi, Y., Sawada, J., Sui, G., Affar el, B., Whetstine, J.R., Lan, F., Ogawa, H., Nakatani, Y., Coordinated histone modifications mediated by a CtBP co-repressor complex (2003) Nature, 422, pp. 735-738; 
Jamora, C., DasGupta, R., Kocieniewski, P., Fuchs, E., Links between signal transduction, transcription and adhesion in epithelial bud development (2003) Nature, 422, pp. 317-322; 
Vandewalle, C., Comijn, J., De Craene, B., Vermassen, P., Bruyneel, E., Andersen, H., Tulchinsky, E., Berx, G., SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions (2005) Nucleic Acids Res., 33, pp. 6566-6578; 
Aigner, K., Dampier, B., Descovich, L., Mikula, M., Sultan, A., Schreiber, M., Mikulits, W., Eger, A., The transcription factor ZEB1 (deltaEF1) promotes tumour cell dedifferentiation by repressing master regulators of epithelial polarity (2007) Oncogene, 26, pp. 6979-6988; 
Spaderna, S., Schmalhofer, O., Wahlbuhl, M., Dimmler, A., Bauer, K., Sultan, A., Hlubek, F., Brabletz, T., The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer (2008) Cancer Res., 68, pp. 537-544; 
Graff, J.R., Herman, J.G., Lapidus, R.G., Chopra, H., Xu, R., Jarrard, D.F., Isaacs, W.B., Baylin, S.B., E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas (1995) Cancer Res., 55, pp. 5195-5199; 
Shimomura, Y., Wajid, M., Shapiro, L., Christiano, A.M., P-cadherin is a p63 target gene with a crucial role in the developing human limb bud and hair follicle (2008) Development, 135, pp. 743-753; 
Carroll, D.K., Carroll, J.S., Leong, C.O., Cheng, F., Brown, M., Mills, A.A., Brugge, J.S., Ellisen, L.W., P63 regulates an adhesion programme and cell survival in epithelial cells (2006) Nat. Cell Biol., 8, pp. 551-561; 
Faraldo, M.M., Teuliere, J., Deugnier, M.A., Birchmeier, W., Huelsken, J., Thiery, J.P., Cano, A., Glukhova, M.A., Beta-catenin regulates P-cadherin expression in mammary basal epithelial cells (2007) FEBS Lett., 581, pp. 831-836; 
Le, T.L., Yap, A.S., Stow, J.L., Recycling of E-cadherin: a potential mechanism for regulating cadherin dynamics (1999) J. Cell Biol., 146, pp. 219-232; 
Bryant, D.M., Stow, J.L., The ins and outs of E-cadherin trafficking (2004) Trends Cell Biol., 14, pp. 427-434; 
Hinck, L., Nathke, I.S., Papkoff, J., Nelson, W.J., Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly (1994) J. Cell Biol., 125, pp. 1327-1340; 
Chen, Y.T., Stewart, D.B., Nelson, W.J., Coupling assembly of the E-cadherin/beta-catenin complex to efficient endoplasmic reticulum exit and basal-lateral membrane targeting of E-cadherin in polarized MDCK cells (1999) J. Cell Biol., 144, pp. 687-699; 
Lock, J.G., Stow, J.L., Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin (2005) Mol. Biol. Cell, 16, pp. 1744-1755; 
Bryant, D.M., Kerr, M.C., Hammond, L.A., Joseph, S.R., Mostov, K.E., Teasdale, R.D., Stow, J.L., EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin (2007) J. Cell Sci., 120, pp. 1818-1828; 
Ling, K., Bairstow, S.F., Carbonara, C., Turbin, D.A., Huntsman, D.G., Anderson, R.A., Type I gamma phosphatidylinositol phosphate kinase modulates adherens junction and E-cadherin trafficking via a direct interaction with mu 1B adaptin (2007) J. Cell Biol., 176, pp. 343-353; 
Mosesson, Y., Mills, G.B., Yarden, Y., Derailed endocytosis: an emerging feature of cancer (2008) Nat. Rev. Cancer, 8, pp. 835-850; 
Ireton, R.C., Davis, M.A., van Hengel, J., Mariner, D.J., Barnes, K., Thoreson, M.A., Anastasiadis, P.Z., Reynolds, A.B., A novel role for p120 catenin in E-cadherin function (2002) J. Cell Biol., 159, pp. 465-476; 
Davis, M.A., Ireton, R.C., Reynolds, A.B., A core function for p120-catenin in cadherin turnover (2003) J. Cell Biol., 163, pp. 525-534; 
Xiao, K., Garner, J., Buckley, K.M., Vincent, P.A., Chiasson, C.M., Dejana, E., Faundez, V., Kowalczyk, A.P., P120-Catenin regulates clathrin-dependent endocytosis of VE-cadherin (2005) Mol. Biol. Cell, 16, pp. 5141-5151; 
Taniuchi, K., Nakagawa, H., Hosokawa, M., Nakamura, T., Eguchi, H., Ohigashi, H., Ishikawa, O., Nakamura, Y., Overexpressed P-cadherin/CDH3 promotes motility of pancreatic cancer cells by interacting with p120ctn and activating rho-family GTPases (2005) Cancer Res., 65, pp. 3092-3099; 
Paredes, J., Stove, C., Stove, V., Milanezi, F., Van Marck, V., Derycke, L., Mareel, M., Schmitt, F., P-cadherin is up-regulated by the antiestrogen ICI 182,780 and promotes invasion of human breast cancer cells (2004) Cancer Res., 64, pp. 8309-8317; 
Palacios, F., Price, L., Schweitzer, J., Collard, J.G., D{\textquoteright}Souza-Schorey, C., An essential role for ARF6-regulated membrane traffic in adherens junction turnover and epithelial cell migration (2001) EMBO J., 20, pp. 4973-4986; 
Figueiredo, J., Simoes-Correia, J., Soderberg, O., Suriano, G., Seruca, R., ADP-ribosylation factor 6 mediates E-cadherin recovery by chemical chaperones (2011) PLoS One, 6, pp. e23188; 
Palacios, F., Schweitzer, J.K., Boshans, R.L., D{\textquoteright}Souza-Schorey, C., ARF6-GTP recruits Nm23-H1 to facilitate dynamin-mediated endocytosis during adherens junctions disassembly (2002) Nat. Cell Biol., 4, pp. 929-936; 
Otsuki, Y., Tanaka, M., Yoshii, S., Kawazoe, N., Nakaya, K., Sugimura, H., Tumor metastasis suppressor nm23H1 regulates Rac1 GTPase by interaction with Tiam1 (2001) Proc. Natl. Acad. Sci. U. S. A., 98, pp. 4385-4390; 
Morishige, M., Hashimoto, S., Ogawa, E., Toda, Y., Kotani, H., Hirose, M., Wei, S., Sabe, H., GEP100 links epidermal growth factor receptor signalling to Arf6 activation to induce breast cancer invasion (2008) Nat. Cell Biol., 10, pp. 85-92; 
Daniel, J.M., Reynolds, A.B., Tyrosine phosphorylation and cadherin/catenin function (1997) Bioessays, 19, pp. 883-891; 
Palacios, F., Tushir, J.S., Fujita, Y., D{\textquoteright}Souza-Schorey, C., Lysosomal targeting of E-cadherin: a unique mechanism for the down-regulation of cell-cell adhesion during epithelial to mesenchymal transitions (2005) Mol. Cell. Biol., 25, pp. 389-402; 
Fujita, Y., Krause, G., Scheffner, M., Zechner, D., Leddy, H.E., Behrens, J., Sommer, T., Birchmeier, W., Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex (2002) Nat. Cell Biol., 4, pp. 222-231; 
Pinho, S.S., Seruca, R., Gartner, F., Yamaguchi, Y., Gu, J., Taniguchi, N., Reis, C.A., Modulation of E-cadherin function and dysfunction by N-glycosylation (2011) Cell. Mol. Life Sci., 68, pp. 1011-1020; 
Zhu, W., Leber, B., Andrews, D.W., Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis (2001) EMBO J., 20, pp. 5999-6007; 
Zhou, F., Su, J., Fu, L., Yang, Y., Zhang, L., Wang, L., Zhao, H., Zha, X., Unglycosylation at Asn-633 made extracellular domain of E-cadherin folded incorrectly and arrested in endoplasmic reticulum, then sequentially degraded by ERAD (2008) Glycoconj. J., 25, pp. 727-740; 
Liwosz, A., Lei, T., Kukuruzinska, M.A., N-glycosylation affects the molecular organization and stability of E-cadherin junctions (2006) J. Biol. Chem., 281, pp. 23138-23149; 
Pinho, S.S., Reis, C.A., Paredes, J., Magalhaes, A.M., Ferreira, A.C., Figueiredo, J., Xiaogang, W., Seruca, R., The role of N-acetylglucosaminyltransferase III and V in the post-transcriptional modifications of E-cadherin (2009) Hum. Mol. Genet., 18, pp. 2599-2608; 
Pinho, S.S., Oliveira, P., Cabral, J., Carvalho, S., Huntsman, D., Gartner, F., Seruca, R., Oliveira, C., Loss and recovery of Mgat3 and GnT-III mediated E-cadherin N-glycosylation is a mechanism involved in epithelial-mesenchymal-epithelial transitions (2012) PLoS One, 7, pp. e33191; 
Osumi, D., Takahashi, M., Miyoshi, E., Yokoe, S., Lee, S.H., Noda, K., Nakamori, S., Taniguchi, N., Core fucosylation of E-cadherin enhances cell-cell adhesion in human colon carcinoma WiDr cells (2009) Cancer Sci., 100, pp. 888-895; 
Takeichi, M., Functional correlation between cell adhesive properties and some cell surface proteins (1977) J. Cell Biol., 75, pp. 464-474; 
Hyafil, F., Morello, D., Babinet, C., Jacob, F., A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos (1980) Cell, 21, pp. 927-934; 
Ogou, S., Okada, T.S., Takeichi, M., Cleavage stage mouse embryos share a common cell adhesion system with teratocarcinoma cells (1982) Dev. Biol., 92, pp. 521-528; 
Takeichi, M., The cadherins: cell-cell adhesion molecules controlling animal morphogenesis (1988) Development, 102, pp. 639-655; 
Fleming, T.P., Javed, Q., Hay, M., Epithelial differentiation and intercellular junction formation in the mouse early embryo (1992) Dev. Suppl., pp. 105-112; 
Aplin, J.D., Jones, C.J., Harris, L.K., Adhesion molecules in human trophoblast-a review. I. Villous trophoblast (2009) Placenta, 30, pp. 293-298; 
Nose, A., Takeichi, M., A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos (1986) J. Cell Biol., 103, pp. 2649-2658; 
Larue, L., Ohsugi, M., Hirchenhain, J., Kemler, R., E-cadherin null mutant embryos fail to form a trophectoderm epithelium (1994) Proc. Natl. Acad. Sci. U. S. A., 91, pp. 8263-8267; 
Riethmacher, D., Brinkmann, V., Birchmeier, C., A targeted mutation in the mouse E-cadherin gene results in defective preimplantation development (1995) Proc. Natl. Acad. Sci. U. S. A., 92, pp. 855-859; 
Radice, G.L., Ferreira-Cornwell, M.C., Robinson, S.D., Rayburn, H., Chodosh, L.A., Takeichi, M., Hynes, R.O., Precocious mammary gland development in P-cadherin-deficient mice (1997) J. Cell Biol., 139, pp. 1025-1032; 
Frebourg, T., Oliveira, C., Hochain, P., Karam, R., Manouvrier, S., Graziadio, C., Vekemans, M., Seruca, R., Cleft lip/palate and CDH1/E-cadherin mutations in families with hereditary diffuse gastric cancer (2006) J. Med. Genet., 43, pp. 138-142; 
Kjaer, K.W., Hansen, L., Schwabe, G.C., Marques-de-Faria, A.P., Eiberg, H., Mundlos, S., Tommerup, N., Rosenberg, T., Distinct CDH3 mutations cause ectodermal dysplasia, ectrodactyly, macular dystrophy (EEM syndrome) (2005) J. Med. Genet., 42, pp. 292-298; 
Sprecher, E., Bergman, R., Richard, G., Lurie, R., Shalev, S., Petronius, D., Shalata, A., Szargel, R., Hypotrichosis with juvenile macular dystrophy is caused by a mutation in CDH3, encoding P-cadherin (2001) Nat. Genet., 29, pp. 134-136; 
Nelson, W.J., Epithelial cell polarity from the outside looking in (2003) News Physiol. Sci., 18, pp. 143-146; 
Hirai, Y., Nose, A., Kobayashi, S., Takeichi, M., Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. II. Skin morphogenesis (1989) Development, 105, pp. 271-277; 
Berx, G., Van Roy, F., The E-cadherin/catenin complex: an important gatekeeper in breast cancer tumorigenesis and malignant progression (2001) Breast Cancer Res., 3, pp. 289-293; 
Shimoyama, Y., Hirohashi, S., Expression of E- and P-cadherin in gastric carcinomas (1991) Cancer Res., 51, pp. 2185-2192; 
Hines, M.D., Jin, H.C., Wheelock, M.J., Jensen, P.J., Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes (1999) J. Cell Sci., 112 (PART 24), pp. 4569-4579; 
Ribeiro, A.S., Albergaria, A., Sousa, B., Correia, A.L., Bracke, M., Seruca, R., Schmitt, F.C., Paredes, J., Extracellular cleavage and shedding of P-cadherin: a mechanism underlying the invasive behaviour of breast cancer cells (2010) Oncogene, 29, pp. 392-402; 
Moll, R., Mitze, M., Frixen, U.H., Birchmeier, W., Differential loss of E-cadherin expression in infiltrating ductal and lobular breast carcinomas (1993) Am. J. Pathol., 143, pp. 1731-1742; 
Gamallo, C., Palacios, J., Suarez, A., Pizarro, A., Navarro, P., Quintanilla, M., Cano, A., Correlation of E-cadherin expression with differentiation grade and histological type in breast carcinoma (1993) Am. J. Pathol., 142, pp. 987-993; 
Reis-Filho, J.S., Cancela Paredes, J., Milanezi, F., Schmitt, F.C., Clinicopathologic implications of E-cadherin reactivity in patients with lobular carcinoma in situ of the breast (2002) Cancer, 94, pp. 2114-2115; 
author reply 2115-2116Weigelt, B., Geyer, F.C., Natrajan, R., Lopez-Garcia, M.A., Ahmad, A.S., Savage, K., Kreike, B., Reis-Filho, J.S., The molecular underpinning of lobular histological growth pattern: a genome-wide transcriptomic analysis of invasive lobular carcinomas and grade- and molecular subtype-matched invasive ductal carcinomas of no special type (2010) J. Pathol., 220, pp. 45-57; 
Simpson, P.T., Reis-Filho, J.S., Lambros, M.B., Jones, C., Steele, D., Mackay, A., Iravani, M., Lakhani, S.R., Molecular profiling pleomorphic lobular carcinomas of the breast: evidence for a common molecular genetic pathway with classic lobular carcinomas (2008) J. Pathol., 215, pp. 231-244; 
Mahler-Araujo, B., Savage, K., Parry, S., Reis-Filho, J.S., Reduction of E-cadherin expression is associated with non-lobular breast carcinomas of basal-like and triple negative phenotype (2008) J. Clin. Pathol., 61, pp. 615-620; 
Lopes, N., Carvalho, J., Duraes, C., Sousa, B., Gomes, M., Costa, J.L., Oliveira, C., Schmitt, F., 1Alpha,25-dihydroxyvitamin D3 induces de novo E-cadherin expression in triple-negative breast cancer cells by CDH1-promoter demethylation (2012) Anticancer. Res., 32, pp. 249-257; 
Berx, G., Cleton-Jansen, A.M., Strumane, K., de Leeuw, W.J., Nollet, F., van Roy, F., Cornelisse, C., E-cadherin is inactivated in a majority of invasive human lobular breast cancers by truncation mutations throughout its extracellular domain (1996) Oncogene, 13, pp. 1919-1925; 
Keller, G., Vogelsang, H., Becker, I., Hutter, J., Ott, K., Candidus, S., Grundei, T., Hofler, H., Diffuse type gastric and lobular breast carcinoma in a familial gastric cancer patient with an E-cadherin germline mutation (1999) Am. J. Pathol., 155, pp. 337-342; 
Schrader, K.A., Masciari, S., Boyd, N., Wiyrick, S., Kaurah, P., Senz, J., Burke, W., Huntsman, D.G., Hereditary diffuse gastric cancer: association with lobular breast cancer (2008) Fam. Cancer, 7, pp. 73-82; 
Becker, K.F., Atkinson, M.J., Reich, U., Becker, I., Nekarda, H., Siewert, J.R., Hofler, H., E-cadherin gene mutations provide clues to diffuse type gastric carcinomas (1994) Cancer Res., 54, pp. 3845-3852; 
Becker, K.F., Kremmer, E., Eulitz, M., Becker, I., Handschuh, G., Schuhmacher, C., Muller, W., Hofler, H., Analysis of E-cadherin in diffuse-type gastric cancer using a mutation-specific monoclonal antibody (1999) Am. J. Pathol., 155, pp. 1803-1809; 
Machado, J.C., Carneiro, F., Beck, S., Rossi, S., Lopes, J., Taveira-Gomes, A., Cardoso-Oliveira, M., E-cadherin expression is correlated with the isolated cell/diffuse histotype and with features of biological aggressiveness of gastric carcinoma (1998) Int. J. Surg. Pathol., 6, pp. 135-144; 
Carneiro, F., Machado, J.C., Seruca, R., Sobrinho-Simoes, M., E-cadherin changes in gastric carcimona (1999) Histopathology, 35, pp. 477-478; 
Carvalho, J., van Grieken, N.C., Pereira, P.M., Sousa, S., Tijssen, M., Buffart, T.E., Diosdado, B., Oliveira, C., Lack of microRNA-101 causes E-cadherin functional deregulation through EZH2 upregulation in intestinal gastric cancer (2012) J. Pathol.; 
Machado, J.C., Oliveira, C., Carvalho, R., Soares, P., Berx, G., Caldas, C., Seruca, R., Sobrinho-Simoes, M., E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma (2001) Oncogene, 20, pp. 1525-1528; 
Guilford, P., Hopkins, J., Harraway, J., McLeod, M., McLeod, N., Harawira, P., Taite, H., Reeve, A.E., E-cadherin germline mutations in familial gastric cancer (1998) Nature, 392, pp. 402-405; 
Oliveira, C., Senz, J., Kaurah, P., Pinheiro, H., Sanges, R., Haegert, A., Corso, G., Huntsman, D., Germline CDH1 deletions in hereditary diffuse gastric cancer families (2009) Hum. Mol. Genet., 18, pp. 1545-1555; 
Pinheiro, H., Bordeira-Carrico, R., Seixas, S., Carvalho, J., Senz, J., Oliveira, P., Inacio, P., Oliveira, C., Allele-specific CDH1 downregulation and hereditary diffuse gastric cancer (2010) Hum. Mol. Genet., 19, pp. 943-952; 
Guilford, P., Humar, B., Blair, V., Hereditary diffuse gastric cancer: translation of CDH1 germline mutations into clinical practice (2010) Gastric Cancer, 13, pp. 1-10; 
Suriano, G., Seixas, S., Rocha, J., Seruca, R., A model to infer the pathogenic significance of CDH1 germline missense variants (2006) J. Mol. Med., 84, pp. 1023-1031; 
Suriano, G., Oliveira, C., Ferreira, P., Machado, J.C., Bordin, M.C., De Wever, O., Bruyneel, E.A., Seruca, R., Identification of CDH1 germline missense mutations associated with functional inactivation of the E-cadherin protein in young gastric cancer probands (2003) Hum. Mol. Genet., 12, pp. 575-582; 
Suriano, G., Oliveira, M.J., Huntsman, D., Mateus, A.R., Ferreira, P., Casares, F., Oliveira, C., Seruca, R., E-cadherin germline missense mutations and cell phenotype: evidence for the independence of cell invasion on the motile capabilities of the cells (2003) Hum. Mol. Genet., 12, pp. 3007-3016; 
Brooks-Wilson, A.R., Kaurah, P., Suriano, G., Leach, S., Senz, J., Grehan, N., Butterfield, Y.S., Huntsman, D., Germline E-cadherin mutations in hereditary diffuse gastric cancer: assessment of 42 new families and review of genetic screening criteria (2004) J. Med. Genet., 41, pp. 508-517; 
Kaurah, P., MacMillan, A., Boyd, N., Senz, J., De Luca, A., Chun, N., Suriano, G., Huntsman, D., Founder and recurrent CDH1 mutations in families with hereditary diffuse gastric cancer (2007) JAMA, 297, pp. 2360-2372; 
Corso, G., Roviello, F., Paredes, J., Pedrazzani, C., Novais, M., Correia, J., Marrelli, D., Suriano, G., Characterization of the P373L E-cadherin germline missense mutation and implication for clinical management (2007) Eur. J. Surg. Oncol., 33, pp. 1061-1067; 
More, H., Humar, B., Weber, W., Ward, R., Christian, A., Lintott, C., Graziano, F., Guilford, P., Identification of seven novel germline mutations in the human E-cadherin (CDH1) gene (2007) Hum. Mutat., 28, p. 203; 
Oliveira, C., de Bruin, J., Nabais, S., Ligtenberg, M., Moutinho, C., Nagengast, F.M., Seruca, R., Carneiro, F., Intragenic deletion of CDH1 as the inactivating mechanism of the wild-type allele in an HDGC tumour (2004) Oncogene, 23, pp. 2236-2240; 
Barber, M., Murrell, A., Ito, Y., Maia, A.T., Hyland, S., Oliveira, C., Save, V., Fitzgerald, R.C., Mechanisms and sequelae of E-cadherin silencing in hereditary diffuse gastric cancer (2008) J. Pathol., 216, pp. 295-306; 
Oliveira, C., Sousa, S., Pinheiro, H., Karam, R., Bordeira-Carrico, R., Senz, J., Kaurah, P., Seruca, R., Quantification of epigenetic and genetic 2nd hits in CDH1 during hereditary diffuse gastric cancer syndrome progression (2009) Gastroenterology, 136, pp. 2137-2148; 
Grady, W.M., Willis, J., Guilford, P.J., Dunbier, A.K., Toro, T.T., Lynch, H., Wiesner, G., Markowitz, S., Methylation of the CDH1 promoter as the second genetic hit in hereditary diffuse gastric cancer (2000) Nat. Genet., 26, pp. 16-17; 
Peinado, H., Ballestar, E., Esteller, M., Cano, A., Snail mediates E-cadherin repression by the recruitment of the Sin3A/histone deacetylase 1 (HDAC1)/HDAC2 complex (2004) Mol. Cell. Biol., 24, pp. 306-319; 
Fujii, S., Ochiai, A., Enhancer of zeste homolog 2 downregulates E-cadherin by mediating histone H3 methylation in gastric cancer cells (2008) Cancer Sci., 99, pp. 738-746; 
Cao, Q., Yu, J., Dhanasekaran, S.M., Kim, J.H., Mani, R.S., Tomlins, S.A., Mehra, R., Chinnaiyan, A.M., Repression of E-cadherin by the polycomb group protein EZH2 in cancer (2008) Oncogene, 27, pp. 7274-7284; 
Pena, C., Garcia, J.M., Silva, J., Garcia, V., Rodriguez, R., Alonso, I., Millan, I., Bonilla, F., E-cadherin and vitamin D receptor regulation by SNAIL and ZEB1 in colon cancer: clinicopathological correlations (2005) Hum. Mol. Genet., 14, pp. 3361-3370; 
Liu, Y., Hong, Y., Zhao, Y., Ismail, T.M., Wong, Y., Eu, K.W., Histone H3 (lys-9) deacetylation is associated with transcriptional silencing of E-cadherin in colorectal cancer cell lines (2008) Cancer Invest., 26, pp. 575-582; 
von Burstin, J., Eser, S., Paul, M.C., Seidler, B., Brandl, M., Messer, M., von Werder, A., Saur, D., E-cadherin regulates metastasis of pancreatic cancer in vivo and is suppressed by a SNAIL/HDAC1/HDAC2 repressor complex (2009) Gastroenterology, 137, pp. 361-371. , (371 e361-365); 
Herranz, N., Pasini, D., Diaz, V.M., Franci, C., Gutierrez, A., Dave, N., Escriva, M., Peiro, S., Polycomb complex 2 is required for E-cadherin repression by the Snail1 transcription factor (2008) Mol. Cell. Biol., 28, pp. 4772-4781; 
Ambros, V., The functions of animal microRNAs (2004) Nature, 431, pp. 350-355; 
He, L., Hannon, G.J., MicroRNAs: small RNAs with a big role in gene regulation (2004) Nat. Rev. Genet., 5, pp. 522-531; 
Hurteau, G.J., Carlson, J.A., Spivack, S.D., Brock, G.J., Overexpression of the microRNA hsa-miR-200c leads to reduced expression of transcription factor 8 and increased expression of E-cadherin (2007) Cancer Res., 67, pp. 7972-7976; 
Korpal, M., Lee, E.S., Hu, G., Kang, Y., The miR-200 family inhibits epithelial-mesenchymal transition and cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2 (2008) J. Biol. Chem., 283, pp. 14910-14914; 
Gregory, P.A., Bert, A.G., Paterson, E.L., Barry, S.C., Tsykin, A., Farshid, G., Vadas, M.A., Goodall, G.J., The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 (2008) Nat. Cell Biol., 10, pp. 593-601; 
Park, S.M., Gaur, A.B., Lengyel, E., Peter, M.E., The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2 (2008) Genes Dev., 22, pp. 894-907; 
Tan, H.X., Wang, Q., Chen, L.Z., Huang, X.H., Chen, J.S., Fu, X.H., Cao, L.Q., Zhang, L.J., MicroRNA-9 reduces cell invasion and E-cadherin secretion in SK-Hep-1 cell (2010) Med. Oncol., 27, pp. 654-660; 
Ma, L., Young, J., Prabhala, H., Pan, E., Mestdagh, P., Muth, D., Teruya-Feldstein, J., Weinberg, R.A., MiR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis (2010) Nat. Cell Biol., 12, pp. 247-256; 
Oki, Y., Issa, J.P., Epigenetic mechanisms in AML-a target for therapy (2010) Cancer Treat. Res., 145, pp. 19-40; 
Simoes-Correia, J., Figueiredo, J., Oliveira, C., van Hengel, J., Seruca, R., van Roy, F., Suriano, G., Endoplasmic reticulum quality control: a new mechanism of E-cadherin regulation and its implication in cancer (2008) Hum. Mol. Genet., 17, pp. 3566-3576; 
Sitia, R., Braakman, I., Quality control in the endoplasmic reticulum protein factory (2003) Nature, 426, pp. 891-894; 
Meusser, B., Hirsch, C., Jarosch, E., Sommer, T., ERAD: the long road to destruction (2005) Nat. Cell Biol., 7, pp. 766-772; 
van Hengel, J., van Roy, F., Diverse functions of p120ctn in tumors (2007) Biochim. Biophys. Acta, 1773, pp. 78-88; 
Jawhari, A.U., Noda, M., Pignatelli, M., Farthing, M., Up-regulated cytoplasmic expression, with reduced membranous distribution, of the src substrate p120(ctn) in gastric carcinoma (1999) J. Pathol., 189, pp. 180-185; 
Karayiannakis, A.J., Syrigos, K.N., Alexiou, D., Kalahanis, N., Rosenberg, T., Bastounis, E., Pignatelli, M., Expression patterns of the novel catenin p120cas in gastrointestinal cancers (1999) Anticancer. Res., 19, pp. 4401-4405; 
Karatzas, G., Karayiannakis, A.J., Syrigos, K.N., Chatzigianni, E., Papanikolaou, S., Simatos, G., Papanikolaou, D., Bogris, S., Expression patterns of the E-cadherin-catenin cell-cell adhesion complex in gastric cancer (2000) Hepatogastroenterology, 47, pp. 1465-1469; 
Apweiler, R., Hermjakob, H., Sharon, N., On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database (1999) Biochim. Biophys. Acta, 1473, pp. 4-8; 
Dube, D.H., Bertozzi, C.R., Glycans in cancer and inflammation-potential for therapeutics and diagnostics (2005) Nat. Rev. Drug Discov., 4, pp. 477-488; 
Pinho, S.S., Osorio, H., Nita-Lazar, M., Gomes, J., Lopes, C., Gartner, F., Reis, C.A., Role of E-cadherin N-glycosylation profile in a mammary tumor model (2009) Biochem. Biophys. Res. Commun., 379, pp. 1091-1096; 
Iijima, J., Zhao, Y., Isaji, T., Kameyama, A., Nakaya, S., Wang, X., Ihara, H., Gu, J., Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion (2006) J. Biol. Chem., 281, pp. 13038-13046; 
Yoshimura, M., Ihara, Y., Matsuzawa, Y., Taniguchi, N., Aberrant glycosylation of E-cadherin enhances cell-cell binding to suppress metastasis (1996) J. Biol. Chem., 271, pp. 13811-13815; 
Giles, R.H., van Es, J.H., Clevers, H., Caught up in a Wnt storm: Wnt signaling in cancer (2003) Biochim. Biophys. Acta, 1653, pp. 1-24; 
Reynolds, A.B., Roczniak-Ferguson, A., Emerging roles for p120-catenin in cell adhesion and cancer (2004) Oncogene, 23, pp. 7947-7956; 
Christofori, G., Changing neighbours, changing behaviour: cell adhesion molecule-mediated signalling during tumour progression (2003) EMBO J., 22, pp. 2318-2323; 
Gottardi, C.J., Wong, E., Gumbiner, B.M., E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner (2001) J. Cell Biol., 153, pp. 1049-1060; 
Stockinger, A., Eger, A., Wolf, J., Beug, H., Foisner, R., E-cadherin regulates cell growth by modulating proliferation-dependent beta-catenin transcriptional activity (2001) J. Cell Biol., 154, pp. 1185-1196; 
Suriano, G., Mulholland, D., de Wever, O., Ferreira, P., Mateus, A.R., Bruyneel, E., Nelson, C.C., Seruca, R., The intracellular E-cadherin germline mutation V832 M lacks the ability to mediate cell-cell adhesion and to suppress invasion (2003) Oncogene, 22, pp. 5716-5719; 
Sahai, E., Marshall, C.J., RHO-GTPases and cancer (2002) Nat. Rev. Cancer, 2, pp. 133-142; 
Deplazes, J., Fuchs, M., Rauser, S., Genth, H., Lengyel, E., Busch, R., Luber, B., Rac1 and Rho contribute to the migratory and invasive phenotype associated with somatic E-cadherin mutation (2009) Hum. Mol. Genet., 18, pp. 3632-3644; 
Mateus, A.R., Seruca, R., Machado, J.C., Keller, G., Oliveira, M.J., Suriano, G., Luber, B., EGFR regulates RhoA-GTP dependent cell motility in E-cadherin mutant cells (2007) Hum. Mol. Genet., 16, pp. 1639-1647; 
Mateus, A.R., Simoes-Correia, J., Figueiredo, J., Heindl, S., Alves, C.C., Suriano, G., Luber, B., Seruca, R., E-cadherin mutations and cell motility: a genotype-phenotype correlation (2009) Exp. Cell Res., 315, pp. 1393-1402; 
Crepaldi, T., Pollack, A.L., Prat, M., Zborek, A., Mostov, K., Comoglio, P.M., Targeting of the SF/HGF receptor to the basolateral domain of polarized epithelial cells (1994) J. Cell Biol., 125, pp. 313-320; 
Pece, S., Gutkind, J.S., Signaling from E-cadherins to the MAPK pathway by the recruitment and activation of epidermal growth factor receptors upon cell-cell contact formation (2000) J. Biol. Chem., 275, pp. 41227-41233; 
Thiery, J.P., Epithelial-mesenchymal transitions in tumour progression (2002) Nat. Rev. Cancer, 2, pp. 442-454; 
Qian, X., Karpova, T., Sheppard, A.M., McNally, J., Lowy, D.R., E-cadherin-mediated adhesion inhibits ligand-dependent activation of diverse receptor tyrosine kinases (2004) EMBO J., 23, pp. 1739-1748; 
Takahashi, K., Suzuki, K., Density-dependent inhibition of growth involves prevention of EGF receptor activation by E-cadherin-mediated cell-cell adhesion (1996) Exp. Cell Res., 226, pp. 214-222; 
Mook, O.R., Frederiks, W.M., Van Noorden, C.J., The role of gelatinases in colorectal cancer progression and metastasis (2004) Biochim. Biophys. Acta, 1705, pp. 69-89; 
Munshi, H.G., Ghosh, S., Mukhopadhyay, S., Wu, Y.I., Sen, R., Green, K.J., Stack, M.S., Proteinase suppression by E-cadherin-mediated cell-cell attachment in premalignant oral keratinocytes (2002) J. Biol. Chem., 277, pp. 38159-38167; 
Lochter, A., Galosy, S., Muschler, J., Freedman, N., Werb, Z., Bissell, M.J., Matrix metalloproteinase stromelysin-1 triggers a cascade of molecular alterations that leads to stable epithelial-to-mesenchymal conversion and a premalignant phenotype in mammary epithelial cells (1997) J. Cell Biol., 139, pp. 1861-1872; 
Davies, G., Jiang, W.G., Mason, M.D., Matrilysin mediates extracellular cleavage of E-cadherin from prostate cancer cells: a key mechanism in hepatocyte growth factor/scatter factor-induced cell-cell dissociation and in vitro invasion (2001) Clin. Cancer Res., 7, pp. 3289-3297; 
Noe, V., Fingleton, B., Jacobs, K., Crawford, H.C., Vermeulen, S., Steelant, W., Bruyneel, E., Mareel, M., Release of an invasion promoter E-cadherin fragment by matrilysin and stromelysin-1 (2001) J. Cell Sci., 114, pp. 111-118; 
Ryniers, F., Stove, C., Goethals, M., Brackenier, L., Noe, V., Bracke, M., Vandekerckhove, J., Bruyneel, E., Plasmin produces an E-cadherin fragment that stimulates cancer cell invasion (2002) Biol. Chem., 383, pp. 159-165; 
Comoglio, P.M., Boccaccio, C., Trusolino, L., Interactions between growth factor receptors and adhesion molecules: breaking the rules (2003) Curr. Opin. Cell Biol., 15, pp. 565-571; 
Ferreira, P., Oliveira, M.J., Beraldi, E., Mateus, A.R., Nakajima, T., Gleave, M., Yokota, J., Suriano, G., Loss of functional E-cadherin renders cells more resistant to the apoptotic agent taxol in vitro (2005) Exp. Cell Res., 310, pp. 99-104; 
Ferreira, A.C., Suriano, G., Mendes, N., Gomes, B., Wen, X., Carneiro, F., Seruca, R., Machado, J.C., E-cadherin impairment increases cell survival through Notch-dependent upregulation of Bcl-2 (2012) Hum. Mol. Genet., 21, pp. 334-343; 
Huang, F.Y., Chan, A.O., Rashid, A., Wong, D.K., Cho, C.H., Yuen, M.F., Helicobacter pylori induces promoter methylation of E-cadherin via interleukin-1beta activation of nitric oxide production in gastric cancer cells (2012) Cancer; 
Chan, A.O., Lam, S.K., Wong, B.C., Wong, W.M., Yuen, M.F., Yeung, Y.H., Hui, W.M., Kwong, Y.L., Promoter methylation of E-cadherin gene in gastric mucosa associated with Helicobacter pylori infection and in gastric cancer (2003) Gut, 52, pp. 502-506; 
Leung, W.K., Man, E.P., Yu, J., Go, M.Y., To, K.F., Yamaoka, Y., Cheng, V.Y., Sung, J.J., Effects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomach (2006) Clin. Cancer Res., 12, pp. 3216-3221; 
Perri, F., Cotugno, R., Piepoli, A., Merla, A., Quitadamo, M., Gentile, A., Pilotto, A., Andriulli, A., Aberrant DNA methylation in non-neoplastic gastric mucosa of H. pylori infected patients and effect of eradication (2007) Am. J. Gastroenterol., 102, pp. 1361-1371; 
Chan, A.O., Peng, J.Z., Lam, S.K., Lai, K.C., Yuen, M.F., Cheung, H.K., Kwong, Y.L., Wong, B.C., Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermethylation (2006) Gut, 55, pp. 463-468; 
Schirrmeister, W., Gnad, T., Wex, T., Higashiyama, S., Wolke, C., Naumann, M., Lendeckel, U., Ectodomain shedding of E-cadherin and c-Met is induced by Helicobacter pylori infection (2009) Exp. Cell Res., 315, pp. 3500-3508; 
Hoy, B., Lower, M., Weydig, C., Carra, G., Tegtmeyer, N., Geppert, T., Schroder, P., Wessler, S., Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E-cadherin to disrupt intercellular adhesion (2010) EMBO Rep., 11, pp. 798-804; 
O{\textquoteright}Connor, P.M., Lapointe, T.K., Jackson, S., Beck, P.L., Jones, N.L., Buret, A.G., Helicobacter pylori activates calpain via toll-like receptor 2 to disrupt adherens junctions in human gastric epithelial cells (2011) Infect. Immun., 79, pp. 3887-3894; 
Conlin, V.S., Curtis, S.B., Zhao, Y., Moore, E.D., Smith, V.C., Meloche, R.M., Finlay, B.B., Buchan, A.M., Helicobacter pylori infection targets adherens junction regulatory proteins and results in increased rates of migration in human gastric epithelial cells (2004) Infect. Immun., 72, pp. 5181-5192; 
Suzuki, M., Mimuro, H., Suzuki, T., Park, M., Yamamoto, T., Sasakawa, C., Interaction of CagA with Crk plays an important role in Helicobacter pylori-induced loss of gastric epithelial cell adhesion (2005) J. Exp. Med., 202, pp. 1235-1247; 
Franco, A.T., Israel, D.A., Washington, M.K., Krishna, U., Fox, J.G., Rogers, A.B., Neish, A.S., Peek, R.M., Activation of beta-catenin by carcinogenic Helicobacter pylori (2005) Proc. Natl. Acad. Sci. U. S. A., 102, pp. 10646-10651; 
Murata-Kamiya, N., Kurashima, Y., Teishikata, Y., Yamahashi, Y., Saito, Y., Higashi, H., Aburatani, H., Hatakeyama, M., Helicobacter pylori CagA interacts with E-cadherin and deregulates the beta-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells (2007) Oncogene, 26, pp. 4617-4626; 
Sokolova, O., Bozko, P.M., Naumann, M., Helicobacter pylori suppresses glycogen synthase kinase 3beta to promote beta-catenin activity (2008) J. Biol. Chem., 283, pp. 29367-29374; 
Ogden, S.R., Wroblewski, L.E., Weydig, C., Romero-Gallo, J., O{\textquoteright}Brien, D.P., Israel, D.A., Krishna, U.S., Peek, R.M., P120 and Kaiso regulate Helicobacter pylori-induced expression of matrix metalloproteinase-7 (2008) Mol. Biol. Cell, 19, pp. 4110-4121; 
Bebb, J.R., Leach, L., Zaitoun, A., Hand, N., Letley, D.P., Thomas, R., Atherton, J.C., Effects of Helicobacter pylori on the cadherin-catenin complex (2006) J. Clin. Pathol., 59, pp. 1261-1266; 
Oliveira, M.J., Costa, A.M., Costa, A.C., Ferreira, R.M., Sampaio, P., Machado, J.C., Seruca, R., Figueiredo, C., CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell-invasive phenotype (2009) J. Infect. Dis., 200, pp. 745-755; 
Oliveira, M.J., Costa, A.C., Costa, A.M., Henriques, L., Suriano, G., Atherton, J.C., Machado, J.C., Figueiredo, C., Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system-dependent manner (2006) J. Biol. Chem., 281, pp. 34888-34896; 
Jang, A.C., Starz-Gaiano, M., Montell, D.J., Modeling migration and metastasis in Drosophila (2007) J. Mammary Gland Biol. Neoplasia, 12, pp. 103-114; 
Pereira, P.S., Teixeira, A., Pinho, S., Ferreira, P., Fernandes, J., Oliveira, C., Seruca, R., Casares, F., E-cadherin missense mutations, associated with hereditary diffuse gastric cancer (HDGC) syndrome, display distinct invasive behaviors and genetic interactions with the Wnt and Notch pathways in Drosophila epithelia (2006) Hum. Mol. Genet., 15, pp. 1704-1712; 
Caldeira, J., Pereira, P.S., Suriano, G., Casares, F., Using fruitflies to help understand the molecular mechanisms of human hereditary diffuse gastric cancer (2009) Int. J. Dev. Biol., 53, pp. 1557-1561; 
Humar, B., Blair, V., Charlton, A., More, H., Martin, I., Guilford, P., E-cadherin deficiency initiates gastric signet-ring cell carcinoma in mice and man (2009) Cancer Res., 69, pp. 2050-2056; 
Mbalaviele, G., Dunstan, C.R., Sasaki, A., Williams, P.J., Mundy, G.R., Yoneda, T., E-cadherin expression in human breast cancer cells suppresses the development of osteolytic bone metastases in an experimental metastasis model (1996) Cancer Res., 56, pp. 4063-4070; 
Perl, A.K., Wilgenbus, P., Dahl, U., Semb, H., Christofori, G., A causal role for E-cadherin in the transition from adenoma to carcinoma (1998) Nature, 392, pp. 190-193; 
Kuwabara, Y., Yamada, T., Yamazaki, K., Du, W.L., Banno, K., Aoki, D., Sakamoto, M., Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis (2008) Cancer Sci., 99, pp. 1933-1939; 
Shimoyama, Y., Hirohashi, S., Hirano, S., Noguchi, M., Shimosato, Y., Takeichi, M., Abe, O., Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas (1989) Cancer Res., 49, pp. 2128-2133; 
Sanders, D.S., Bruton, R., Darnton, S.J., Casson, A.G., Hanson, I., Williams, H.K., Jankowski, J., Sequential changes in cadherin-catenin expression associated with the progression and heterogeneity of primary oesophageal squamous carcinoma (1998) Int. J. Cancer, 79, pp. 573-579; 
Rocha, A.S., Soares, P., Machado, J.C., Maximo, V., Fonseca, E., Franssila, K., Sobrinho-Simoes, M., Mucoepidermoid carcinoma of the thyroid: a tumour histotype characterised by P-cadherin neoexpression and marked abnormalities of E-cadherin/catenins complex (2002) Virchows Arch., 440, pp. 498-504; 
Kim, M.A., Jung, E.J., Lee, H.S., Lee, H.E., Yang, H.K., Oh, D.Y., Bang, Y.J., Kim, W.H., P-cadherin expression in gastric carcinoma: its regulation mechanism and prognostic significance (2010) Hum. Pathol., 41, pp. 877-885; 
Imai, K., Hirata, S., Irie, A., Senju, S., Ikuta, Y., Yokomine, K., Harao, M., Nishimura, Y., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers (2008) Clin. Cancer Res., 14, pp. 6487-6495; 
Lo Muzio, L., Campisi, G., Farina, A., Rubini, C., Pannone, G., Serpico, R., Laino, G., Carinci, F., P-cadherin expression and survival rate in oral squamous cell carcinoma: an immunohistochemical study (2005) BMC Cancer, 5, p. 63; 
Han, A.C., Edelson, M.I., Peralta Soler, A., Knudsen, K.A., Lifschitz-Mercer, B., Czernobilsky, B., Rosenblum, N.G., Salazar, H., Cadherin expression in glandular tumors of the cervix (2000) Cancer, 89, pp. 2053-2058; 
Longatto Filho, A., Albergaria, A., Paredes, J., Moreira, M.A., Milanezi, F., Schmitt, F.C., P-cadherin expression in glandular lesions of the uterine cervix detected by liquid-based cytology (2005) Cytopathology, 16, pp. 88-93; 
Stefansson, I.M., Salvesen, H.B., Akslen, L.A., Prognostic impact of alterations in P-cadherin expression and related cell adhesion markers in endometrial cancer (2004) J. Clin. Oncol., 22, pp. 1242-1252; 
Moreno-Bueno, G., Hardisson, D., Sarrio, D., Sanchez, C., Cassia, R., Prat, J., Herman, J.G., Palacios, J., Abnormalities of E- and P-cadherin and catenin (beta-, gamma-catenin, and p120ctn) expression in endometrial cancer and endometrial atypical hyperplasia (2003) J. Pathol., 199, pp. 471-478; 
Quattrocchi, L., Green, A.R., Martin, S., Durrant, L., Deen, S., The cadherin switch in ovarian high-grade serous carcinoma is associated with disease progression (2011) Virchows Arch., 459, pp. 21-29; 
Sarrio, D., Moreno-Bueno, G., Sanchez-Estevez, C., Banon-Rodriguez, I., Hernandez-Cortes, G., Hardisson, D., Palacios, J., Expression of cadherins and catenins correlates with distinct histologic types of ovarian carcinomas (2006) Hum. Pathol., 37, pp. 1042-1049; 
Soler, A.P., Harner, G.D., Knudsen, K.A., McBrearty, F.X., Grujic, E., Salazar, H., Han, A.C., Keshgegian, A.A., Expression of P-cadherin identifies prostate-specific-antigen-negative cells in epithelial tissues of male sexual accessory organs and in prostatic carcinomas. Implications for prostate cancer biology (1997) Am. J. Pathol., 151, pp. 471-478; 
Bryan, R.T., Atherfold, P.A., Yeo, Y., Jones, L.J., Harrison, R.F., Wallace, D.M., Jankowski, J.A., Cadherin switching dictates the biology of transitional cell carcinoma of the bladder: ex vivo and in vitro studies (2008) J. Pathol., 215, pp. 184-194; 
Matos, I., Dufloth, R., Alvarenga, M., Zeferino, L.C., Schmitt, F., P63, cytokeratin 5, and P-cadherin: three molecular markers to distinguish basal phenotype in breast carcinomas (2005) Virchows Arch., 447, pp. 688-694; 
Arnes, J.B., Brunet, J.S., Stefansson, I., Begin, L.R., Wong, N., Chappuis, P.O., Akslen, L.A., Foulkes, W.D., Placental cadherin and the basal epithelial phenotype of BRCA1-related breast cancer (2005) Clin. Cancer Res., 11, pp. 4003-4011; 
Palacios, J., Benito, N., Pizarro, A., Suarez, A., Espada, J., Cano, A., Gamallo, C., Anomalous expression of P-cadherin in breast carcinoma. Correlation with E-cadherin expression and pathological features (1995) Am. J. Pathol., 146, pp. 605-612; 
Peralta Soler, A., Knudsen, K.A., Salazar, H., Han, A.C., Keshgegian, A.A., P-cadherin expression in breast carcinoma indicates poor survival (1999) Cancer, 86, pp. 1263-1272; 
Paredes, J., Milanezi, F., Reis-Filho, J.S., Leitao, D., Athanazio, D., Schmitt, F., Aberrant P-cadherin expression: is it associated with estrogen-independent growth in breast cancer? (2002) Pathol. Res. Pract., 198, pp. 795-801; 
Kovacs, A., Dhillon, J., Walker, R.A., Expression of P-cadherin, but not E-cadherin or N-cadherin, relates to pathological and functional differentiation of breast carcinomas (2003) Mol. Pathol., 56, pp. 318-322; 
Gamallo, C., Moreno-Bueno, G., Sarrio, D., Calero, F., Hardisson, D., Palacios, J., The prognostic significance of P-cadherin in infiltrating ductal breast carcinoma (2001) Mod. Pathol., 14, pp. 650-654; 
Paredes, J., Correia, A.L., Ribeiro, A.S., Milanezi, F., Cameselle-Teijeiro, J., Schmitt, F.C., Breast carcinomas that co-express E- and P-cadherin are associated with p120-catenin cytoplasmic localisation and poor patient survival (2008) J. Clin. Pathol., 61, pp. 856-862; 
Foulkes, W.D., Stefansson, I.M., Chappuis, P.O., Begin, L.R., Goffin, J.R., Wong, N., Trudel, M., Akslen, L.A., Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer (2003) J. Natl. Cancer Inst., 95, pp. 1482-1485; 
Lakhani, S.R., Reis-Filho, J.S., Fulford, L., Penault-Llorca, F., van der Vijver, M., Parry, S., Bishop, T., Easton, D.F., Prediction of BRCA1 status in patients with breast cancer using estrogen receptor and basal phenotype (2005) Clin. Cancer Res., 11, pp. 5175-5180; 
Palacios, J., Honrado, E., Osorio, A., Cazorla, A., Sarrio, D., Barroso, A., Rodriguez, S., Benitez, J., Immunohistochemical characteristics defined by tissue microarray of hereditary breast cancer not attributable to BRCA1 or BRCA2 mutations: differences from breast carcinomas arising in BRCA1 and BRCA2 mutation carriers (2003) Clin. Cancer Res., 9, pp. 3606-3614; 
Palacios, J., Honrado, E., Osorio, A., Cazorla, A., Sarrio, D., Barroso, A., Rodriguez, S., Benitez, J., Phenotypic characterization of BRCA1 and BRCA2 tumors based in a tissue microarray study with 37 immunohistochemical markers (2005) Breast Cancer Res. Treat., 90, pp. 5-14; 
Gorski, J.J., James, C.R., Quinn, J.E., Stewart, G.E., Staunton, K.C., Buckley, N.E., McDyer, F.A., Harkin, D.P., BRCA1 transcriptionally regulates genes associated with the basal-like phenotype in breast cancer (2010) Breast Cancer Res. Treat., 122, pp. 721-731; 
Albergaria, A., Ribeiro, A.S., Pinho, S., Milanezi, F., Carneiro, V., Sousa, B., Sousa, S., Schmitt, F., ICI 182,780 induces P-cadherin overexpression in breast cancer cells through chromatin remodelling at the promoter level: a role for C/EBPbeta in CDH3 gene activation (2010) Hum. Mol. Genet., 19, pp. 2554-2566; 
Sato, N., Fukushima, N., Maitra, A., Matsubayashi, H., Yeo, C.J., Cameron, J.L., Hruban, R.H., Goggins, M., Discovery of novel targets for aberrant methylation in pancreatic carcinoma using high-throughput microarrays (2003) Cancer Res., 63, pp. 3735-3742; 
Milicic, A., Harrison, L.A., Goodlad, R.A., Hardy, R.G., Nicholson, A.M., Presz, M., Sieber, O., Jankowski, J.A., Ectopic expression of P-cadherin correlates with promoter hypomethylation early in colorectal carcinogenesis and enhanced intestinal crypt fission in vivo (2008) Cancer Res., 68, pp. 7760-7768; 
Van Marck, V., Stove, C., Van Den Bossche, K., Stove, V., Paredes, J., Vander Haeghen, Y., Bracke, M., P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma (2005) Cancer Res., 65, pp. 8774-8783; 
Sarrio, D., Palacios, J., Hergueta-Redondo, M., Gomez-Lopez, G., Cano, A., Moreno-Bueno, G., Functional characterization of E- and P-cadherin in invasive breast cancer cells (2009) BMC Cancer, 9, p. 74; 
Bauer, K., Dowejko, A., Bosserhoff, A.K., Reichert, T.E., Bauer, R.J., P-cadherin induces an epithelial-like phenotype in oral squamous cell carcinoma by GSK-3beta-mediated Snail phosphorylation (2009) Carcinogenesis, 30, pp. 1781-1788; 
Bauer, K., Dowejko, A., Bosserhoff, A.K., Reichert, T.E., Bauer, R., Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line (2011) Carcinogenesis, 32, pp. 935-943; 
Mandeville, J.A., Silva Neto, B., Vanni, A.J., Smith, G.L., Rieger-Christ, K.M., Zeheb, R., Loda, M., Summerhayes, I.C., P-cadherin as a prognostic indicator and a modulator of migratory behaviour in bladder carcinoma cells (2008) BJU Int., 102, pp. 1707-1714; 
Cheung, L.W., Leung, P.C., Wong, A.S., Cadherin switching and activation of p120 catenin signaling are mediators of gonadotropin-releasing hormone to promote tumor cell migration and invasion in ovarian cancer (2010) Oncogene, 29, pp. 2427-2440; 
Reynolds, A.B., Daniel, J.M., Mo, Y.Y., Wu, J., Zhang, Z., The novel catenin p120cas binds classical cadherins and induces an unusual morphological phenotype in NIH3T3 fibroblasts (1996) Exp. Cell Res., 225, pp. 328-337; 
Baki, L., Marambaud, P., Efthimiopoulos, S., Georgakopoulos, A., Wen, P., Cui, W., Shioi, J., Robakis, N.K., Presenilin-1 binds cytoplasmic epithelial cadherin, inhibits cadherin/p120 association, and regulates stability and function of the cadherin/catenin adhesion complex (2001) Proc. Natl. Acad. Sci. U. S. A., 98, pp. 2381-2386; 
Jacobs, K., Van Gele, M., Forsyth, R., Brochez, L., Vanhoecke, B., De Wever, O., Bracke, M., P-cadherin counteracts myosin II-B function: implications in melanoma progression (2010) Mol. Cancer, 9, p. 255; 
Radice, G.L., Sauer, C.L., Kostetskii, I., Peralta Soler, A., Knudsen, K.A., Inappropriate P-cadherin expression in the mouse mammary epithelium is compatible with normal mammary gland function (2003) Differentiation, 71, pp. 361-373; 
Gregory, D.J., Mikhaylova, L., Fedulov, A.V., Selective DNA demethylation by fusion of TDG with a sequence-specific DNA-binding domain (2012) Epigenetics, 7; 
Zhang, C.C., Yan, Z., Zhang, Q., Kuszpit, K., Zasadny, K., Qiu, M., Painter, C.L., VanArsdale, T., PF-03732010: a fully human monoclonal antibody against P-cadherin with antitumor and antimetastatic activity (2010) Clin. Cancer Res., 16, pp. 5177-5188
},
	month = {2012///},
	pages = {297 - 311},
	abstract = {E-cadherin and P-cadherin are major contributors to cell-cell adhesion in epithelial tissues, playing pivotal roles in important morphogenetic and differentiation processes during development, and in maintaining integrity and homeostasis in adult tissues. It is now generally accepted that alterations in these two molecules are observed during tumour progression of most carcinomas. Genetic or epigenetic alterations in E- and P-cadherin-encoding genes (CDH1 and CDH3, respectively), or alterations in their proteins expression, often result in tissue disorder, cellular de-differentiation, increased invasiveness of tumour cells and ultimately in metastasis. In this review, we will discuss the major properties of E- and P-cadherin molecules, its regulation in normal tissue, and their alterations and role in cancer, with a specific focus on gastric and breast cancer models. {\textcopyright} 2012 Elsevier B.V.},
	keywords = {antineoplastic activity, antineoplastic agent, Breast, breast cancer, Cancer, cancer chemotherapy, cancer model, cancer survival, drug effect, E-cadherin, Gastric, gene expression regulation, gene function, gene location, gene overexpression, gene structure, genetic association, genetic code, Helicobacter infection, Hereditary diffuse gastric cancer, human, in vivo study, loss of function mutation, lymph node metastasis, malignant neoplastic disease, molecular dynamics, molecular pathology, molecularly targeted therapy, nonhuman, P cadherin, P cadherin gene, P-cadherin, pf 03732010, priority journal, protein expression, protein function, protein inhibitor, protein processing, review, signal transduction, stomach cancer, transcription regulation, tumor gene, unclassified drug, uvomorulin, uvomorulin gene, vasculotropin inhibitor},
	isbn = {0304419X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861800037\&partnerID=40\&md5=2d52be63f4168a561b1bda1315e1f429},
	author = {Paredes, J. and Figueiredo, J. and Albergaria, A. and Oliveira, P. and Carvalho, J. and Ribeiro, A.S. and Caldeira, J. and Costa, A.M. and Sim{\~o}es-Correia, J. and Oliveira, M.J. and Pinheiro, H. and Pinho, S.S. and Mateus, R. and Reis, C.A. and Leite, M. and Fernandes, M.S. and Schmitt, F. and Carneiro, F. and Figueiredo, C. and Oliveira, C. and Seruca, R.}
}
@book {2213,
	title = {FTIR analysis and cytotoxicity test of titanium dioxide nanoparticles},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {493-494},
	year = {2012},
	note = {Conference code: 87411Export Date: 21 November 2012Source: ScopusCODEN: KEMAEdoi: 10.4028/www.scientific.net/KEM.493-494.768Language of Original Document: EnglishCorrespondence Address: Rodrigues, L.R.; Department of Materials Engineering, Faculty of Mechanical Engineering, State University of Campinas, Campinas, S{\~a}o Paulo, Brazil; email: leo5002@fem.unicamp.brReferences: Pavlova, A., Barloti, J., Teters, V., Locs, J., Berzina-Cimdina, L., (2009) Processing and Application of Ceramics, 3 (4), pp. 187-190;Naldoni, A., Minguzzi, A., Vertova, A., Santo, V.D., Borgesec, L., Bianchi, C.L., (2010) J. Mater. Chem., 21, pp. 400-407; 
Li, Y., Jiang, Y., Liu, F., Ren, F., Zhao, G., Leng, X., (2011) Food Hydrocolloids, 25 (5), pp. 1098-1104; 
Topuz, B.B., Gunduz, G., Mavis, B., {\c C}olak, U., (2011) Dyes and Pigments, 90 (2), pp. 123-128; 
Nag, N., Guin, D., Basak, P., Manorama, S.V., (2008) Materials Research Bulletin, 43 (12), pp. 3270-3285; 
Yu, W., Qiu, J., Zhang, F., (2011) Colloids and Surfaces B: Biointerfaces, 84 (2), pp. 400-405; 
Wang, J., Li, S., Yan, W., Tse, S.D., Yao, Q., (2011) Proceeding of the Combustion Institute, 33 (2), pp. 1925-1932; 
Ma, P.X., (2008) Advanced Drug Delivery Reviews, 60, pp. 184-198; 
Liu, X., Smith, L.A., Hu, J., Ma, P.X., (2009) Biomaterials, 30, pp. 2252-2258; 
Qui, D., Yang, L., Yin, Y., Wang, A., (2011) Surface and Coatings Technology, 205 (10), pp. 3280-3284; 
Amin, R., Hwang, S., Park, S.H., (2011) NANO: Brief Reports and Reviews, 6 (2), pp. 101-111; 
Allounia, A.E., Cimpan, M.R., Hol, P.J., Skodvind, T., Gjerdet, N.R., (2009) Colloids and Surfaces B: Biointerfaces, 68, pp. 83-87; 
Akurati, K.K., (2008) Synthesis of TiO 2 Based Nanoparticles for Photocatalytic Applications, , 1st edition. Cuvillier Verlag G{\"o}ttingen; 
Nag, M., Guin, D., Manorama, S.V., (2007) Materials Research Bulletin, 9, pp. 1691-1704; 
Birks, L.S., Friedman, H., (1946) Journal of Applied Physics, 17, pp. 687-692; 
Rodas, A.C.D., Maizato, M.J.S., Leiner, A.A., Pitombo, R.N.M., Polakiewicz, B., Beppu, M.M., Higa, O.Z., (2008) Artificial Organs, 32 (4), pp. 272-276; 
Zhang, J.Y., Boyd, I.W., O{\textquoteright}Sullivan, B.J., Hurley, P.K., Kelly, P.V., S{\'e}nateur, J.P., (2002) Journal of Non-Crystalline Solids, 303, pp. 134-138; 
Arconada, N., Dur{\'a}n, A., Su{\'a}rez, S., Portela, R., Coronado, J.M., S{\'a}nchez, B., Castro, Y., (2009) Applied Catalysis B: Environmental, 86 (1-2), pp. 1-7; 
Wang, J., Li, R.H., Zhang, Z.H., Sun, W., Wang, X.F., Xing, Z.Q., Xu, R., Zhang, X.D., (2008) Inorganic Materials, 44 (6), pp. 608-614; 
Orendorz, A., Brodyanski, A., L{\"o}sch, J., Bai, L.H., Chen, Z.H., Le, Y.K., Ziegler, C., Gnaser, H., (2007) Surface Science, 601, pp. 4390-4394
},
	month = {2012///},
	pages = {768 - 774},
	address = {Istanbul},
	abstract = {Titanium dioxide is a material widely used in electronics industry and little explored in the biomedical area, which is the objective of this work. Nowadays one can find surgical instruments coated with thin films that have bactericidal properties when they are activated in the presence of ultraviolet light. For crystalline phase control TiO                        2 was calcinated at 500{\textdegree}C. The crystallite mean size for sample calcinated at 500{\textdegree}C was 27nm. With the results of cytotoxicity it is possible to say that biomedical applications are possible. Electron microscopy images showed nanoparticles obtained by sol-gel process and the compounds were identified by FTIR analysis. Raman spectroscopy confirmed the existence of anatase titania phase and X-ray diffraction showed this material to be composed of a crystalline phase. X-ray fluorescence identified chemical contaminants. {\textcopyright} (2012) Trans Tech Publications.
},
	keywords = {Anatase titania, Bactericidal properties, Bioceramics, Biomedical applications, Chemical contaminants, Crystalline materials, Crystalline phase, Crystallite size, Cytotoxicity, Cytotoxicity test, Electron microscopy images, Electronics industry, FTIR, FTIR analysis, Gels, Mean size, Medical applications, nanoparticles, Nanotechnology, Oxides, Raman spectroscopy, Scherrer equation, Scherrer equations, Sol-gel process, Sol-gel with sucrose, Sol-gels, Sols, Sugar (sucrose), Surgical equipment, Surgical instrument, TiO, Titanium, Titanium dioxide, Titanium dioxide nanoparticles, Ultra-violet light, Ultraviolet radiation, X ray diffraction, X ray fluorescence},
	isbn = {10139826 (ISSN); 9783037852552 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-81555221106\&partnerID=40\&md5=6e76ec1290c649a4d981ebf5e2fbf4b3},
	author = {Rodrigues, L.R. and Dias, C.G.B.T. and Ceragioli, H.J. and Rodas, A.C.D. and Monteiro, F.J.M. and Zavaglia, C.A.C.}
}
@article {2206,
	title = {Gradient convergence filters and a phase congruency approach for in vivo cell nuclei detection},
	journal = {Machine Vision and ApplicationsMach Vision Appl},
	volume = {23},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: MVAPEdoi: 10.1007/s00138-012-0407-7Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB-Instituto de Engenharia Biom{\'e}dica, Oporto, Portugal; email: quelhas@fe.up.ptReferences: Bleau, A., Leon, L.J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370. , DOI 10.1006/cviu.1999.0822;Bresson, X., Vandergheynst, P., Thiran, J., A priori information in image segmentation: Energy functional based on shape statistical model and image information (2003) Proceedings of International Conference on Image Processing, pp. 428-428. , ICIP; 
Bunyak, F., Palaniappan, K., Nath, S.K., Baskin, T.I., Dong, G., Quantitative cellmotility for in vitrowound healing using level setbased active contour tracking (2011) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 1-4; 
Byun, J., Verardo, M.R., Sumengen, B., Lewis, G.P., Manjunath, B.S., Fisher, S.K., Automated tool for the detection of cell nuclei in digital microscopic images: Application to retinal images (2006) Mol. Vis., 12, pp. 949-960; 
Campilho, A., Garcia, B., Toorn, H.V.D., Wijk, H.V., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48 (4), pp. 619-627. , DOI 10.1111/j.1365-313X.2006.02892.x; 
Chan, T.F., Vese, L.A., Active contours without edges (2001) IEEE Transactions on Image Processing, 10 (2), pp. 266-277. , DOI 10.1109/83.902291; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell e nuclei in time-lapse microscopy (2006) IEEE Trans. Biomed. Eng., 53 (4), pp. 762-766; 
Chen, Y., Ladi, E., Herzmark, P., Robey, E., Roysam, B., Automated 5-d analysis of cell migration and interaction in the thymic cortex from time-lapse sequences of 3-d multi-channel multi-photon images (2009) J. Immunol. Methods, 340 (1), pp. 65-80; 
Chen, Y., Quelhas, P., Campilho, A., Low frame rate cell tracking: A delaunay graph matching approach (2011) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 1-4; 
Clocksin, W., Automatic segmentation of overlapping nuclei with high background variation using robust estimation and flexible contour models (2003) Proceedings of the International Conference on Image Analysis and Processing, pp. 682-687; 
Dewitte, W., Murray, J., The plant cell cycle (2003) Annu. Rev. Plant Biol., 54, pp. 235-297; 
Fang, W., Chan, K.L., Incorporating shape prior into geodesic active contours for detecting partially occluded object (2007) Pattern Recognition, 40 (8), pp. 2163-2172. , DOI 10.1016/j.patcog.2006.12.014, PII S0031320306005292; 
Fok, Y.-L., Chan, J.C.K., Chin, R.T., Automated analysis of nerve-cell images using active contour models (1996) IEEE Transactions on Medical Imaging, 15 (3), pp. 353-368. , PII S0278006296042425; 
Guan, P., Yan, H., Blood cell image segmentation based on the hough transform and fuzzy curve tracing (2011) International Conference on Machine Learning and Cybernetics (ICMLC), 4, pp. 1696-1701; 
Hafiane, A., Bunyak, F., Palaniappan, K., Clustering initiated multiphase active contours and robust separation of nuclei groups for tissue segmentation (2008) International Conference on Pattern Recognition (ICPR), pp. 1-4; 
Hafiane, A., Bunyak, F., Palaniappan, K., Fuzzy clustering and active contours for histopathology image segmentation and nuclei detection (2008) Advanced Concepts for Intelligent Vision Systems LNCS, 5259, pp. 903-914. , Springer, Berlin; 
Han, J., Breckon, T., Randell, D., Landini, G., The application of support vector machine classification to detect cell nuclei for automated microscopy (2010) Mach. Vis. Appl., 23 (1), pp. 15-24; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3d fluorescence microscopy image sequences (2008) Workshop on Bio- Image Informatics: Biological Imaging, ComputerVision AndData Mining; 
Hu, M., Ping, X., Ding, Y., A new active contour model and its application on cell segmentation (2004) 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV), 2, pp. 1104-1107. , TuA7.09 - P0673, 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV); 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. Image Process., 8 (8), pp. 1029-1038; 
Kovesi, P., Image features from phase congruency (1999) Videre, pp. 1-26; 
Kovesi, P., Phase congruency detects corners and edges (2003) Digital Image Computing: Techniques and Applications, pp. 309-318; 
Kube, P., Properties of energy edge detectors (1992) IEEE Conference on ComputerVision and Pattern Recognition (CVPR), pp. 586-591; 
Leibe, B., Leonardis, A., Schiele, B., Combined object categorization and segmentation with an implicit shape model (2004) ECCV Workshop on Statistical Learning in Computer Vision, pp. 17-32; 
Leventon, M., Grimson, W., Faugeras, O., Statistical shape influence in geodesic active contours (2000) Proceedings of IEEE Conference on Computer Vision and Pattern Recognition (CVPR, pp. 316-323; 
Li, C., Xu, C., Gui, C., Fox, M.D., Level set evolution without re-initialization: A new variational formulation (2005) IEEE International Conference on Computer Vision and Pattern Recognition (CVPR), pp. 430-436; 
Li, K., Miller, E.D., Chen, M., Kanade, T., Weiss, L.E., Campbell, P.G., Cell population tracking and lineage construction with spatiotemporal context (2008) Med. Image Anal., 12, pp. 546-566; 
Lindeberg, T., Scale-space theory: A basic tool for analysing structures at different scales (1994) J. Appl. Stat., 21 (2), pp. 224-270; 
Marcuzzo, M., Guichard, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Cell division detection on the Arabidopsis thaliana root (2009) Proceedings of the Iberian Conference on Pattern Recognition and Image Analysis, LNCS, 5524, pp. 168-175. , Springer, Berlin; 
Marcuzzo, M., Quelhas, P., Campilho, A., Maria Mendon{\c c}a, A., Campilho, A., Automated Arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Comput Biol Med, 39 (9), pp. 785-793; 
Marcuzzo, M., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images (2009) Proceedings of the International Conference on Image Analysis and Recognition, 5627, pp. 824-831. , Springer, Berlin; 
Marcuzzo, M., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Tracking of Arabidopsis thaliana root cells in time-lapsemicroscopy (2009) Proceedings of the International Conference on Pattern Recognition (ICPR), pp. 1-4; 
Moore, P., Molloy, D., A survey of computer-based deformable models (2007) International Machine Vision and Image Processing Conference, pp. 55-66; 
Morrone, M., Burr, D., Feature detection in human vision: A phase-dependent energy model (1988) Proc. Roy. Soc. Lond. B Biol. Sci., 235 (1280), pp. 221-245; 
Mosaliganti, K., Gelas, A., Gouaillard, A., Megason, S., Microscopy image analysis: Blob segmentation using geodesic active contours (2009) Insight J.; 
Otsu Nobuyuki, Threshold selection method from gray-level histograms (1979) IEEE Trans Syst Man Cybern, SMC-9 (1), pp. 62-66; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A.C., Detection of lung nodule candidates in chest radiographs (2007) Iberian Conference on Pattern Recognition and Image Analysis, 2, pp. 170-177; 
Perona Pietro, Malik Jitendra, Scale-space and edge detection using anisotropic diffusion (1990) IEEE Transactions on Pattern Analysis and Machine Intelligence, 12 (7), pp. 629-639. , DOI 10.1109/34.56205; 
Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., A thousand words in a scene (2007) IEEE Transactions on Pattern Analysis and Machine Intelligence, 29 (9), pp. 1575-1589. , DOI 10.1109/TPAMI.2007.1155; 
Quelhas, P., Marcuzzo, M., Oliveira, M.J., Mendon{\c c}a, A.M., Campilho, A., Cancer cell detection and invasion depth estimation in brightfield images (2009) Proceedings of the British Machine Vision Conference; 
Quelhas, P., Marcuzzo, M., Mendonca, A.M., Campilho, A., Cell nuclei and cytoplasm joint segmentation using the sliding band filter (2010) IEEE Trans. Med. Imaging, 29 (8), pp. 1463-1473; 
Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., 3d cell nuclei fluorescence quantification using sliding band filter (2010) International Conference on Pattern Recognition (ICPR), pp. 2508-2511; 
Roberts, T., McKenna, S., Du, C.J., Wuyts, N., Valentine, T., Bengough, A., Estimating the motion of plant root cells from in vivo confocal laser scanning microscopy images (2010) Mach. Vis. Appl., 21, pp. 921-939; 
Sanz, L., Dewitte, W., Forzani, C., Patell, F., Nieuwland, J., Wen, B., Quelhas, P., Murray, J.A., The Arabidopsis d-type cyclin cycd2;1 and the inhibitor ick2/krp2 modulate auxin-induced lateral root formation (2011) Plant Cell, 23, pp. 641-660; 
Sivic, J., Zisserman, A., Video google: A text retrieval approach to object matching in videos (2003) Proceedings of the IEEE International Conference on Computer Vision (ICCV), pp. 1-8; 
Tek, F.B., Dempster, A.G., Kale, I., Blood cell segmentation using minimum area watershed and circle radon transformations (2005) Mathematical Morphology: 40 Years On, Computational Imaging and Vision, 30, pp. 441-454. , Ronse, C., Najman, L., Decenci{\`e}re, E. (eds.), Springer, Netherlands; 
Usaj, M., Torkar, D., Kanduser, M., Mikalavcic, D., Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast images (2011) J. Microsc., 241 (3), pp. 303-314; 
Vincent Luc, Soille Pierre, Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Transactions on Pattern Analysis and Machine Intelligence, 13 (6), pp. 583-598. , DOI 10.1109/34.87344; 
Wei Jun, Hagihara Yoshihiro, Kobatake Hidefumi, Detection of cancerous tumors on chest X-ray images - candidate detection filter and its evaluation (1999) IEEE International Conference on Image Processing, 3, pp. 397-401; 
Wei, J., Hagihara, Y., Kobatake, H., Edge detection and skeletonization using quantized localized phase (2009) European Signal Processing Conference (EUSIPCO), pp. 1542-1546; 
Willamowski, J., Arregui, D., Csurka, G., Dance, C.R., Fan, L., Categorizing nine visual classes using local appearance descriptors (2004) ICPR Workshop on Learning for Adaptable Visual Systems, pp. 1-11; 
Willemse, J., Kulikova, O., Jong, H., Bisseling, T., A new wholemount dna quantification method and the analysis of nuclear DNA content in the stem-cell niche of Arabidopsis roots (2008) Plant J., 55 (5), pp. 886-894; 
Xiong, G., Zhou, X., Ji, L., Automated segmentation of drosophila RNAi fluorescence cellular images using deformable models (2006) IEEE Transactions on Circuits and Systems I: Regular Papers, 53 (11), pp. 2415-2424. , DOI 10.1109/TCSI.2006.884461; 
Xiong, G., Zhou, X., Ji, L., Bradley, P., Perrimon, N., Wong, S., Segmentation ofDrosophila RNAI fluorescence images using level sets (2006) Proceedings of IEEE International Conference on Image Processing, pp. 73-76; 
Xue, Q., Degrelle, S., Wang, J., Hue, I., Guillomot, M., A level set based hybrid framework for confocal image segmentation (2008) Proceedings of the IASTED International Conference on Biomedical Engineering, pp. 453-457; 
Yan, P., Zhou, X., Shah, M., Wong, S.T.C., Automatic segmentation of high-throughput RNAi fluorescent cellular images (2008) IEEE Transactions on Information Technology in Biomedicine, 12 (1), pp. 109-117. , DOI 10.1109/TITB.2007.898006; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and Kalman filter in time-lapse microscopy (2006) IEEE Transactions on Circuits and Systems I: Regular Papers, 53 (11), pp. 2405-2414. , DOI 10.1109/TCSI.2006.884469; 
Yi Wang, F.H., Jiankun, H., Fengling, H., Enhanced gradientbased algorithm for the estimation of fingerprint orientation fields Appl. Math. Comput., 185, pp. 823-833. , http://seit.unsw.adfa.edu.au/staff/sites/hu/Sample_Publication/ Elsevier_Wang.pdf; 
Yin, Z., Bise, R., Chen, M., Kanade, T., Cell segmentation in microscopy imagery using a bag of local Bayesian classifiers (2010) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 125-128
},
	month = {2012///},
	pages = {623 - 638},
	abstract = {Computational methods used in microscopy cell image analysis have largely augmented the impact of imaging techniques, becoming fundamental for biological research. The understanding of cell regulation processes is very important in biology, and in particular confocal fluorescence imaging plays a relevant role for the in vivo observation of cells. However, most biology researchers still analyze cells by visual inspection alone, which is time consuming and prone to induce subjective bias. This makes automatic cell image analysis essential for large scale, objective studies of cells. While the classic approach for automatic cell analysis is to use image segmentation, for in vivo confocal fluorescence microscopy images of plants, such approach is neither trivial nor is it robust to image quality variations. To analyze plant cells in in vivo confocal fluorescence microscopy images with robustness and increased performance, we propose the use of local convergence filters (LCF). These filters are based in gradient convergence and as such can handle illumination variations, noise and low contrast.We apply a range of existing convergence filters for cell nuclei analysis of the Arabidopsis thaliana plant root tip. To further increase contrast invariance, we present an augmentation to local convergence approaches based on image phase information. Through the use of convergence index filters we improved the results for cell nuclei detection and shape estimation when compared with baseline approaches. Using phase congruency information we were able to further increase performance by 11\% for nuclei detection accuracy and 4\% for shape adaptation. Shape regularization was also applied, but with no significant gain, which indicates shape estimation was good for the applied filters. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Biological research, Cell analysis, Cell images, cell nucleus, Cell regulation, Cell segmentation, Cells, Confocal fluorescence imaging, Confocal fluorescence microscopy, Convergence filters, cytology, Detection accuracy, Estimation, Fluorescence microscopy, Illumination variation, Image analysis, Image filtering, Image segmentation, Imaging techniques, In-vivo, Information use, Local Convergence, Local image filtering, Medical applications, Phase congruency, Phase information, Plant cells, Plant roots, Plants (botany), Quality variation, Shape adaptation, Shape estimation, Shape regularization, Visual inspection},
	isbn = {09328092 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865374061\&partnerID=40\&md5=8e2b458028b5492266f085546a6804d9},
	author = {Esteves, T. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2207,
	title = {Methamphetamine mimics the neurochemical profile of aging in rats and impairs recognition memory},
	journal = {NeuroToxicologyNeuroToxicology},
	volume = {33},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: NRTXDdoi: 10.1016/j.neuro.2012.03.002PubMed ID: 22433442Language of Original Document: EnglishCorrespondence Address: Melo, P.; Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal; email: pmelo@ibmc.up.ptChemicals/CAS: dopamine, 51-61-6, 62-31-7; methamphetamine, 28297-73-6, 51-57-0, 537-46-2, 7632-10-2; serotonin, 50-67-9; sodium chloride, 7647-14-5; Hydroxyindoleacetic Acid, 54-16-0; Methamphetamine, 537-46-2; Serotonin, 50-67-9References: Ali, S.F., David, S.N., Newport, G.D., Age-related susceptibility to MPTP-induced neurotoxicity in mice (1993) Neurotoxicology, 14, pp. 29-34;Alves, E., Binienda, Z., Carvalho, F., Alves, C.J., Fernandes, E., Bastos, M.D.L., Acetyl-l-carnitine provides effective in vivo neuroprotection over 3,4-methylenedioximethamphetamine-induced mitochondrial neurotoxicity in the adolescent rat brain (2009) Neuroscience, 158, pp. 514-523; 
Belcher, A.M., O{\textquoteright}Dell, S.J., Marshall, J.F., A sensitizing regimen of methamphetamine causes impairments in a novelty preference task of object recognition (2006) Behav Brain Res, 170, pp. 167-172; 
Belcher, A.M., Feinstein, E.M., O{\textquoteright}Dell, S.J., Marshall, J.F., Methamphetamine influences on recognition memory: comparison of escalating and single-day dosing regimens (2008) Neuropsychopharmacology, 33, pp. 1453-1463; 
Bergado, J.A., Almaguer, W., Rojas, Y., Capdevila, V., Frey, J.U., Spatial and emotional memory in aged rats: a behavioral-statistical analysis (2011) Neuroscience, 172, pp. 256-269; 
Biagioni, F., Pellegrini, A., Ruggieri, S., Murri, L., Paparelli, A., Fornai, F., Behavioural sensitisation during dopamine replacement therapy in Parkinson{\textquoteright}s disease is reminiscent of the addicted brain (2009) Curr Top Med Chem, 9, pp. 894-902; 
Bisagno, V., Ferguson, D., Luine, V.N., Short toxic methamphetamine schedule impairs object recognition task in male rats (2002) Brain Res, 940, pp. 95-101; 
Boger, H.A., Middaugh, L.D., Patrick, K.S., Ramamoorthy, S., Denehy, E.D., Zhu, H., Long-term consequences of methamphetamine exposure in young adults are exacerbated in glial cell line-derived neurotrophic factor heterozygous mice (2007) J Neurosci, 27, pp. 8816-8825; 
Boger, H.A., Middaugh, L.D., Granholm, A.C., McGinty, J.F., Minocycline restores striatal tyrosine hydroxylase in GDNF heterozygous mice but not in methamphetamine-treated mice (2009) Neurobiol Dis, 33, pp. 459-466; 
Boguszewski, P., Zagrodzka, J., Emotional changes related to age in rats - a behavioral analysis (2002) Behav Brain Res, 133, pp. 323-332; 
Bubenikova-Valesova, V., Kacer, P., Syslova, K., Rambousek, L., Janovsky, M., Schutova, B., Prenatal methamphetamine exposure affects the mesolimbic dopaminergic system and behavior in adult offspring (2009) Int J Dev Neurosci, 27, pp. 525-530; 
Camarasa, J., Rodrigo, T., Pubill, D., Escubedo, E., Memantine is a useful drug to prevent the spatial and non-spatial memory deficits induced by methamphetamine in rats (2010) Pharmacol Res, 62, pp. 450-456; 
Capela, J.P., Carmo, H., Remiao, F., Bastos, M.L., Meisel, A., Carvalho, F., Molecular and cellular mechanisms of ecstasy-induced neurotoxicity: an overview (2009) Mol Neurobiol, 39, pp. 210-271; 
Carfagna, N., Trunzo, F., Moretti, A., Brain catecholamine content and turnover in aging rats (1985) Exp Gerontol, 20, pp. 265-269; 
Carnes, B.A., Staats, D.O., Sonntag, W.E., Does senescence give rise to disease? (2008) Mech Ageing Dev, 129, pp. 693-699; 
Carvalho, F., How bad is accelerated senescence in consumers of drugs of abuse? (2009) Adicciones, 21, pp. 99-104; 
Chang, L., Alicata, D., Ernst, T., Volkow, N., Structural and metabolic brain changes in the striatum associated with methamphetamine abuse (2007) Addiction, 102 (SUPPL. 1), pp. 16-32; 
Cretzmeyer, M., Sarrazin, M.V., Huber, D.L., Block, R.I., Hall, J.A., Treatment of methamphetamine abuse: research findings and clinical directions (2003) J Subst Abuse Treat, 24, pp. 267-277; 
Cubells, J.F., Rayport, S., Rajendran, G., Sulzer, D., Methamphetamine neurotoxicity involves vacuolation of endocytic organelles and dopamine-dependent intracellular oxidative stress (1994) J Neurosci, 14, pp. 2260-2271; 
Dowling, G.J., Weiss, S.R.B., Condon, T.P., Drugs of abuse and the aging brain (2008) Neuropsychopharmacology, 33, pp. 209-218; 
Eisch, A.J., Schmued, L.C., Marshall, J.F., Characterizing cortical neuron injury with Fluoro-Jade labeling after a neurotoxic regimen of methamphetamine (1998) Synapse, 30, pp. 329-333; 
Ennaceur, A., Neave, N., Aggleton, J.P., Spontaneous object recognition and object location memory in rats: the effects of lesions in the cingulate cortices, the medial prefrontal cortex, the cingulum bundle and the fornix (1997) Exp Brain Res, 113, pp. 509-519; 
Ennaceur, A., Michalikova, S., Bradford, A., Ahmed, S., Detailed analysis of the behavior of Lister and Wistar rats in anxiety, object recognition and object location tasks (2005) Behav Brain Res, 159, pp. 247-266; 
Erickson, C.A., Barnes, C.A., The neurobiology of memory changes in normal aging (2003) Exp Gerontol, 38, pp. 61-69; 
Esteban, S., Garau, C., Aparicio, S., Moranta, D., Barcelo, P., Fiol, M.A., Chronic melatonin treatment and its precursor l-tryptophan improve the monoaminergic neurotransmission and related behavior in the aged rat brain (2010) J Pineal Res, 48, pp. 170-177; 
Esteban, S., Garau, C., Aparicio, S., Moranta, D., Barcelo, P., Ramis, M., Improving effects of long-term growth hormone treatment on monoaminergic neurotransmission and related behavioral tests in aged rats (2010) Rejuvenation Res, 13, pp. 707-716; 
Fleckenstein, A.E., Metzger, R.R., Wilkins, D.G., Gibb, J.W., Hanson, G.R., Rapid and reversible effects of methamphetamine on dopamine transporters (1997) J Pharmacol Exp Ther, 282, pp. 834-838; 
Friedman, S.D., Castaneda, E., Hodge, G.K., Long-term monoamine depletion, differential recovery, and subtle behavioral impairment following methamphetamine-induced neurotoxicity (1998) Pharmacol Biochem Behav, 61, pp. 35-44; 
Frost, D.O., Cadet, J.L., Effects of methamphetamine-induced neurotoxicity on the development of neural circuitry: a hypothesis (2000) Brain Res Brain Res Rev, 34, pp. 103-118; 
Fumagalli, F., Gainetdinov, R.R., Wang, Y.M., Valenzano, K.J., Miller, G.W., Caron, M.G., Increased methamphetamine neurotoxicity in heterozygous vesicular monoamine transporter 2 knock-out mice (1999) J Neurosci, 19, pp. 2424-2431; 
Garwood, E.R., Bekele, W., McCulloch, C.E., Christine, C.W., Amphetamine exposure is elevated in Parkinson{\textquoteright}s disease (2006) Neurotoxicology, 27, pp. 1003-1006; 
Gasbarri, A., Verney, C., Innocenzi, R., Campana, E., Pacitti, C., Mesolimbic dopaminergic neurons innervating the hippocampal formation in the rat: a combined retrograde tracing and immunohistochemical study (1994) Brain Res, 668, pp. 71-79; 
Graham, D.G., Oxidative pathways for catecholamines in the genesis of neuromelanin and cytotoxic quinones (1978) Mol Pharmacol, 14, pp. 633-643; 
Guilarte, T.R., Is methamphetamine abuse a risk factor in parkinsonism? (2001) Neurotoxicology, 22, pp. 725-731; 
Harvey, D.C., Lacan, G., Tanious, S.P., Melega, W.P., Recovery from methamphetamine induced long-term nigrostriatal dopaminergic deficits without substantia nigra cell loss (2000) Brain Res, 871, pp. 259-270; 
Hotchkiss, A.J., Gibb, J.W., Long-term effects of multiple doses of methamphetamine on tryptophan hydroxylase and tyrosine hydroxylase activity in rat brain (1980) J Pharmacol Exp Ther, 214, pp. 257-262; 
Jones, S.R., Gainetdinov, R.R., Wightman, R.M., Caron, M.G., Mechanisms of amphetamine action revealed in mice lacking the dopamine transporter (1998) J Neurosci, 18, pp. 1979-1986; 
Kalechstein, A.D., Newton, T.F., Green, M., Methamphetamine dependence is associated with neurocognitive impairment in the initial phases of abstinence (2003) J Neuropsychiatry Clin Neurosci, 15, pp. 215-220; 
Kish, S.J., Shannak, K., Rajput, A., Deck, J.H., Hornykiewicz, O., Aging produces a specific pattern of striatal dopamine loss: implications for the etiology of idiopathic Parkinson{\textquoteright}s disease (1992) J Neurochem, 58, pp. 642-648; 
Koda, L.Y., Gibb, J.W., Adrenal and striatal tyrosine hydroxylase activity after methamphetamine (1973) J Pharmacol Exp Ther, 185, pp. 42-48; 
Lu, P., Mamiya, T., Lu, L.L., Mouri, A., Niwa, M., Kim, H.C., Silibinin attenuates cognitive deficits and decreases of dopamine and serotonin induced by repeated methamphetamine treatment (2010) Behav Brain Res, 207, pp. 387-393; 
Luine, V., Bowling, D., Hearns, M., Spatial memory deficits in aged rats - contributions of monoaminergic systems (1990) Brain Res, 537, pp. 271-278; 
Luparini, M.R., Del Vecchio, A., Barillari, G., Magnani, M., Prosdocimi, M., Cognitive impairment in old rats: a comparison of object displacement, object recognition and water maze (2000) Aging (Milano), 12, pp. 264-273; 
Marshall, J.F., Belcher, A.M., Feinstein, E.M., O{\textquoteright}Dell, S.J., Methamphetamine-induced neural and cognitive changes in rodents (2007) Addiction, 102, pp. 61-69; 
Melega, W.P., Williams, A.E., Schmitz, D.A., DiStefano, E.W., Cho, A.K., Pharmacokinetic and pharmacodynamic analysis of the actions of d-amphetamine and d-methamphetamine on the dopamine terminal (1995) J Pharmacol Exp Ther, 274, pp. 90-96; 
Melo, P., Zanon-Moreno, V., Alves, C.J., Magalhaes, A., Tavares, M.A., Pinazo-Duran, M.D., Oxidative stress response in the adult rat retina and plasma after repeated administration of methamphetamine (2010) Neurochem Int, 56, pp. 431-436; 
Meredith, C.W., Jaffe, C., Ang-Lee, K., Saxon, A.J., Implications of chronic methamphetamine use: a literature review (2005) Harv Rev Psychiatry, 13, pp. 141-154; 
Metzger, R.R., Haughey, H.M., Wilkins, D.G., Gibb, J.W., Hanson, G.R., Fleckenstein, A.E., Methamphetamine-induced rapid decrease in dopamine transporter function: role of dopamine and hyperthermia (2000) J Pharmacol Exp Ther, 295, pp. 1077-1085; 
Miguez, J.M., Aldegunde, M., Paz-Valinas, L., Recio, J., Sanchez-Barcelo, E., Selective changes in the contents of noradrenaline, dopamine and serotonin in rat brain areas during aging (1999) J Neural Transm, 106, pp. 1089-1098; 
Moretti, A., Carfagna, N., Trunzo, F., Effect of aging on monoamines and their metabolites in the rat brain (1987) Neurochem Res, 12, pp. 1035-1039; 
Nagai, T., Takuma, K., Dohniwa, M., Ibi, D., Mizoguchi, H., Kamei, H., Repeated methamphetamine treatment impairs spatial working memory in rats: reversal by clozapine but not haloperidol (2007) Psychopharmacology, 194, pp. 21-32; 
Nash, J.F., Yamamoto, B.K., Methamphetamine neurotoxicity and striatal glutamate release: comparison to 3,4-methylenedioxymethamphetamine (1992) Brain Res, 581, pp. 237-243; 
Nordahl, T.E., Salo, R., Leamon, M., Neuropsychological effects of chronic methamphetamine use on neurotransmitters and cognition. A review (2003) J Neuropsychiatry Clin Neurosci, 15, pp. 317-325; 
Ossowska, K., Disturbances in neurotransmission processes in aging and age-related diseases (1993) Pol J Pharmacol, 45, pp. 109-131; 
Ponzio, F., Brunello, N., Algeri, S., Catecholamine synthesis in brain of ageing rat (1978) J Neurochem, 30, pp. 1617-1620; 
Ponzio, F., Calderini, G., Lomuscio, G., Vantini, G., Toffano, G., Algeri, S., Changes in monoamines and their metabolite levels in some brain regions of aged rats (1982) Neurobiol Aging, 3, pp. 23-29; 
Pradhan, S.N., Central neurotransmitters and aging (1980) Life Sci, 26, pp. 1643-1656; 
Reis, D.J., Ross, R.A., Joh, T.H., Changes in the activity and amounts of enzymes synthesizing catecholamines and acetylcholine in brain, adrenal medulla, and sympathetic ganglia of aged rat and mouse (1977) Brain Res, 136, pp. 465-474; 
Ricaurte, G.A., Schuster, C.R., Seiden, L.S., Long-term effects of repeated methylamphetamine administration on dopamine and serotonin neurons in the rat brain: a regional study (1980) Brain Res, 193, pp. 153-163; 
Ricaurte, G.A., Guillery, R.W., Seiden, L.S., Schuster, C.R., Moore, R.Y., Dopamine nerve terminal degeneration produced by high doses of methylamphetamine in the rat brain (1982) Brain Res, 235, pp. 93-103; 
Ricaurte, G.A., Fuller, R.W., Perry, K.W., Seiden, L.S., Schuster, C.R., Fluoxetine increases long-lasting neostriatal dopamine depletion after administration of d-methamphetamine and d-amphetamine (1983) Neuropharmacology, 22, pp. 1165-1169; 
Ricaurte, G.A., Seiden, L.S., Schuster, C.R., Increased dopamine metabolism in the rat neostriatum after toxic doses of d-methylamphetamine (1983) Neuropharmacology, 22, pp. 1383-1388; 
Robinson, T.E., Yew, J., Paulson, P.E., Camp, D.M., The long-term effects of neurotoxic doses of methamphetamine on the extracellular concentration of dopamine measured with microdialysis in striatum (1990) Neurosci Lett, 110, pp. 193-198; 
Rogers, J.L., De Santis, S., See, R.E., Extended methamphetamine self-administration enhances reinstatement of drug seeking and impairs novel object recognition in rats (2008) Psychopharmacology (Berl), 199, pp. 615-624; 
Sabol, K.E., Roach, J.T., Broom, S.L., Ferreira, C., Preau, M.M., Long-term effects of a high-dose methamphetamine regimen on subsequent methamphetamine-induced dopamine release in vivo (2001) Brain Res, 892, pp. 122-129; 
Salo, R., Ursu, S., Buonocore, M.H., Leamon, M.H., Carter, C., Impaired prefrontal cortical function and disrupted adaptive cognitive control in methamphetamine abusers: a functional magnetic resonance imaging study (2009) Biol Psychiatry, 65, pp. 706-709; 
Santiago, M., Machado, A., Reinoso-Suarez, F., Cano, J., Changes in biogenic amines in rat hippocampus during development and aging (1988) Life Sci, 42, pp. 2503-2508; 
Schroder, N., O{\textquoteright}Dell, S.J., Marshall, J.F., Neurotoxic methamphetamine regimen severely impairs recognition memory in rats (2003) Synapse, 49, pp. 89-96; 
Schwendt, M., Rocha, A., See, R.E., Pacchioni, A.M., McGinty, J.F., Kalivas, P.W., Extended methamphetamine self-administration in rats results in a selective reduction of dopamine transporter levels in the prefrontal cortex and dorsal striatum not accompanied by marked monoaminergic depletion (2009) J Pharmacol Exp Ther, 331, pp. 555-562; 
Scott, J.C., Woods, S.P., Matt, G.E., Meyer, R.A., Heaton, R.K., Atkinson, J.H., Neurocognitive effects of methamphetamine: a critical review and meta-analysis (2007) Neuropsychol Rev, 17, pp. 275-297; 
Seiden, L.S., Sabol, K.E., Ricaurte, G.A., Amphetamine: effects on catecholamine systems and behavior (1993) Annu Rev Pharmacol Toxicol, 33, pp. 639-677; 
Sekine, Y., Iyo, M., Ouchi, Y., Matsunaga, T., Tsukada, H., Okada, H., Methamphetamine-related psychiatric symptoms and reduced brain dopamine transporters studied with PET (2001) Am J Psychiatry, 158, pp. 1206-1214; 
Simon, S.L., Domier, C., Carnell, J., Brethen, P., Rawson, R., Ling, W., Cognitive impairment in individuals currently using methamphetamine (2000) Am J Addict, 9, pp. 222-231; 
Simon, S.L., Dacey, J., Glynn, S., Rawson, R., Ling, W., The effect of relapse on cognition in abstinent methamphetamine abusers (2004) J Subst Abuse Treat, 27, pp. 59-66; 
Treit, D., Fundytus, M., Thigmotaxis as a test for anxiolytic activity in rats (1988) Pharmacol Biochem Behav, 31, pp. 959-962; 
Troen, B.R., The biology of aging (2003) Mt Sinai J Med, 70, pp. 3-22; 
Venero, J.L., Machado, A., Cano, J., Turnover of dopamine and serotonin and their metabolites in the striatum of aged rats (1991) J Neurochem, 56, pp. 1940-1948; 
Venero, J.L., de la Roza, C., Machado, A., Cano, J., Age-related changes on monoamine turnover in hippocampus of rats (1993) Brain Res, 631, pp. 89-96; 
Volkow, N.D., Chang, L., Wang, G.J., Fowler, J.S., Leonido-Yee, M., Franceschi, D., Association of dopamine transporter reduction with psychomotor impairment in methamphetamine abusers (2001) Am J Psychiatry, 158, pp. 377-382; 
Volz, T.J., Fleckenstein, A.E., Hanson, G.R., Methamphetamine-induced alterations in monoamine transport: implications for neurotoxicity, neuroprotection and treatment (2007) Addiction, 102 (SUPPL. 1), pp. 44-48; 
Vorhees, C.V., Reed, T.M., Morford, L.L., Fukumura, M., Wood, S.L., Brown, C.A., Periadolescent rats (P41-50) exhibit increased susceptibility to d-methamphetamine-induced long-term spatial and sequential learning deficits compared to juvenile (P21-30 or P31-40) or adult rats (P51-60) (2005) Neurotoxicol Teratol, 27, pp. 117-134; 
Vupputuri, S., Batuman, V., Muntner, P., Bazzano, L.A., Lefante, J.J., Whelton, P.K., Risk for mild kidney function decline associated with illicit drug use among hypertensive men (2004) Am J Kidney Dis, 43, pp. 629-635; 
Wagner, G.C., Ricaurte, G.A., Johanson, C.E., Schuster, C.R., Seiden, L.S., Amphetamine induces depletion of dopamine and loss of dopamine uptake sites in caudate (1980) Neurology, 30, pp. 547-550; 
Wagner, G.C., Ricaurte, G.A., Seiden, L.S., Schuster, C.R., Miller, R.J., Westley, J., Long-lasting depletions of striatal dopamine and loss of dopamine uptake sites following repeated administration of methamphetamine (1980) Brain Res, 181, pp. 151-160; 
White, I.M., Minamoto, T., Odell, J.R., Mayhorn, J., White, W., Brief exposure to methamphetamine (METH) and phencyclidine (PCP) during late development leads to long-term learning deficits in rats (2009) Brain Res, 1266, pp. 72-86
},
	month = {2012///},
	pages = {491 - 499},
	abstract = {Brain neurochemistry and cognition performance are thought to decline with age. Accumulating data indicate that similar events occur after prolonged methamphetamine (MA) exposure. Using the rat as a model, the present study was designed to uncover common alteration patterns in brain neurochemistry and memory performance between aging and prolonged MA exposure. To this end, animals were treated with a chronic binge MA administration paradigm (20. mg/kg/day from postnatal day 91 to 100). Three-age control groups received isovolumetric saline treatment and were tested at the MA age-matched period, and at 12 and 20 months. We observed that both MA and aged animals presented a long, but not short, time impairment in novelty preference and an increased anxiety-like behavior. Neurochemical analysis indicated similar MA- and age-related impairments in dopamine, serotonin and metabolites in the striatum, prefrontal cortex and hippocampus. Thus, the present data illustrate that MA may be used to mimic age-related effects on neurotransmitter systems and advocate MA treatment as a feasible animal model to study neuronal processes associated with aging. {\textcopyright} 2012 Elsevier Inc.},
	keywords = {Accelerated aging, Age Factors, aging, animal behavior, animal experiment, animal model, animal tissue, Animalia, Animals, anxiety, article, behavior change, Behavior, Animal, Brain, Brain Chemistry, controlled study, corpus striatum, dopamine, Exploratory Behavior, hippocampus, Hydroxyindoleacetic Acid, long term exposure, male, Memory Disorders, mental performance, Methamphetamine, Neurochemistry, neurotransmission, nonhuman, perinatal drug exposure, prefrontal cortex, priority journal, rat, Rats, Rats, Wistar, Rattus, recognition, Recognition (Psychology), Recognition memory, serotonin, sodium chloride, Time Factors},
	isbn = {0161813X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861188767\&partnerID=40\&md5=9c50ef658007fb37150c8b2d6d235d29},
	author = {Melo, P. and Magalh{\~a}es, A. and Alves, C.J. and Tavares, M.A. and De Sousa, L. and Summavielle, T. and Moradas-Ferreira, P.}
}
@article {2178,
	title = {Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration},
	journal = {Dental MaterialsDent. Mater.},
	volume = {28},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: DEMAEdoi: 10.1016/j.dental.2012.09.002Language of Original Document: EnglishCorrespondence Address: Carvalho, A.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: angela.carvalho@ineb.up.ptReferences: Neumann, A., Biomaterials for craniofacial reconstruction (2009) Laryngo- Rhino- Otologie, 88 (SUPPL. 1), pp. 48-S63;Klinge, B., Hultin, M., Berglundh, T., Peri-implantitis (2005) Dental Clinics of North America, 49 (3 SPEC. ISSUE), pp. 661-676. , DOI 10.1016/j.cden.2005.03.007, PII S0011853205000236; 
Hacking, S.A., Khademhosseini, A., Applications of microscale technologies for regenerative dentistry (2009) Journal of Dental Research, 88, pp. 409-421; 
Jung, Y.-G., Wuttiphan, S., Peterson, I.M., Lawn, B.R., Damage modes in dental layer structures (1999) Journal of Dental Research, 78 (4), pp. 887-897; 
Du Preez, L.A., Butow, K.W., Swart, T.J., Implant failure due to titanium hypersensitivity/allergy? Report of a case (2007) SADJ, 62 (22), pp. 24-25; 
Pelaez-Vargas, A., Gallego-Perez, D., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping (2010) Microscopy and Microanalysis, 16, pp. 670-676; 
Bolind, P., Acton, C., Albrektsson, T., Bonding, P., Granstrom, G., Johansson, C., Lindeman, P., Tjellstrom, A., Histologic evaluation of retrieved craniofacial implants (2000) Otolaryngology - Head and Neck Surgery, 123 (1), pp. 140-146; 
Ito, Y., Surface micropatterning to regulate cell functions (1999) Biomaterials, 20, pp. 2333-2342; 
Mendonca, G., Mendonca, D.B., Aragao, F.J., Cooper, L.F., Advancing dental implant surface technology from micron to nanotopography (2008) Biomaterials, 29, pp. 3822-3835; 
Pelaez-Vargas, A., Gallego-Perez, D., Magallanes-Perdomo, M., Fernandes, M.H., Hansford, D.J., De Aza, A.H., Pena, P., Monteiro, F.J., Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants (2011) Dental Materials, 27, pp. 581-589; 
Rocha, S.S.R., Bernardi, A.C.A., Pizzolitto, A.C., Adabo, G.L., Pizzolitto, E.L., Streptococcus mutans attachment on a cast titanium surface (2009) Journal of Materials Research, 12, pp. 41-44; 
Deng, D.M., Ten Cate, J.M., Crielaard, W., The adaptive response of Streptococcus mutans towards oral care products: Involvement of the ClpP serine protease (2007) European Journal of Oral Sciences, 115, pp. 363-370; 
Shemesh, M., Tam, A., Aharoni, R., Steinberg, D., Genetic adaptation of Streptococcus mutans during biofilm formation on different types of surfaces (2010) BMC Microbiology, 10, p. 51; 
Wang, B.Y., Alvarez, P., Hong, J., Kuramitsu, H.K., Periodontal pathogens interfere with quorum-sensing-dependent virulence properties in Streptococcus mutans (2011) Journal of Periodontal Research, 46, pp. 105-110; 
Kumar, P.S., Mason, M.R., Brooker, M.R., O{\textquoteright}Brien, K., Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants (2012) Journal of Clinical Periodontology, 39, pp. 425-433; 
Kohal, R.J., Att, W., Bachle, M., Butz, F., Ceramic abutments and ceramic oral implants. An update (2008) Periodontology 2000, 47, pp. 224-243; 
Inan, O., Secilmis, A., Eraslan, O., Effect of pontic framework design on the fracture resistance of implant-supported all-ceramic fixed partial dentures (2009) Journal of Applied Oral Science, 17, pp. 533-538; 
Yildirim, M., Fischer, H., Marx, R., Edelhoff, D., In vivo fracture resistance of implant-supported all-ceramic restorations (2003) Journal of Prosthetic Dentistry, 90 (4), pp. 325-331. , DOI 10.1016/S0022-3913(03)00514-6; 
Kohal, R.J., Wolkewitz, M., Hinze, M., Han, J.S., Bachle, M., Butz, F., Biomechanical and histological behavior of zirconia implants: An experiment in the rat (2009) Clinical Oral Implants Research, 20, pp. 333-339; 
Scarano, A., Di Carlo, F., Quaranta, M., Piattelli, A., Bone response to zirconia ceramic implants: An experimental study in rabbits (2003) Journal of Oral Implantology, 29, pp. 8-12; 
Oliva, J., Oliva, X., Oliva, J., One-year follow-up of first consecutive 100 zirconia dental implants in human: A comparison of 2 different rough surfaces (2007) International Journal of Oral and Maxillofacial Implants, 22, pp. 430-435; 
Gahlert, M., Gudehus, T., Eichhorn, S., Steinhauser, E., Kniha, H., Erhardt, W., Biomechanical and histomorphometric comparison between zirconia implants with varying surface textures and a titanium implant in the maxilla of miniature pigs (2007) Clinical Oral Implants Research, 18, pp. 662-668; 
Marzolin, C., Smith, S.P., Prentiss, M., Whitesides, G.M., Fabrication of glass microstructures by micro-molding of sol-gel precursors (1998) Advanced Materials, 10 (8), pp. 571-574; 
Gallardo, J., Galliano, P.G., Lopez, J.M.P., Preparation and in vitro evaluation of porous silica gels (2002) Biomaterials, 23 (21), pp. 4277-4284. , DOI 10.1016/S0142-9612(02)00190-4, PII S0142961202001904; 
Hench, L.L., Sol-gel materials for bioceramic applications (1997) Current Opinion in Solid State and Materials Science, 2 (5), pp. 604-610. , PII S1359028697800538; 
Garcia, C., Cere, S., Duran, A., Bioactive coatings prepared by sol-gel on stainless steel 316L (2004) Journal of Non-Crystalline Solids, 348, pp. 218-224; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D.J., Monteiro, F.J., Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process (2009) Key Engineering Materials, 396-398, pp. 303-306; 
Xia, Y., Whitesides, G.M., Soft lithography (1998) Annual Review of Materials Science, 28 (1), pp. 153-184; 
Qin, D., Xia, Y.N., Whitesides, G.M., Soft lithography for micro- and nanoscale patterning (2010) Nature Protocols, 5, pp. 491-502; 
Gutwein, L.G., Webster, T.J., Increased viable osteoblast density in the presence of nanophase compared to conventional alumina and titania particles (2004) Biomaterials, 25 (18), pp. 4175-4183. , DOI 10.1016/j.biomaterials.2003.10.090, PII S0142961203010226; 
Price, R.L., Gutwein, L.G., Kaledin, L., Tepper, F., Webster, T.J., Osteoblast function on nanophase alumina materials: Influence of chemistry, phase, and topography (2003) Journal of Biomedical Materials Research - Part A, 67 (4), pp. 1284-1293; 
Schwartz, Z., Nasazky, E., Boyan, B.D., Surface microtopography regulates osteointegration: The role of implant surface microtopography in osteointegration (2005) Alpha Omegan, 98, pp. 9-19; 
Flemming, R.G., Murphy, C.J., Abrams, G.A., Goodman, S.L., Nealey, P.F., Effects of synthetic micro- and nano-structured surfaces on cell behavior (1999) Biomaterials, 20 (6), pp. 573-588. , DOI 10.1016/S0142-9612(98)00209-9, PII S0142961298002099; 
Bettinger, C.J., Langer, R., Borenstein, J.T., Engineering substrate topography at the micro- and nanoscale to control cell function (2009) Angewandte Chemie International Edition, 48, pp. 5406-5415; 
Le Guehennec, L., Soueidan, A., Layrolle, P., Amouriq, Y., Surface treatments of titanium dental implants for rapid osseointegration (2007) Dental Materials, 23 (7), pp. 844-854. , DOI 10.1016/j.dental.2006.06.025, PII S0109564106001850; 
Craighead, H.G., James, C.D., Turner, A.M.P., Chemical and topographical patterning for directed cell attachment (2001) Current Opinion in Solid State and Materials Science, 5 (2-3), pp. 177-184. , DOI 10.1016/S1359-0286(01)00005-5, PII S1359028601000055; 
Xiao, J., Zhou, H., Zhao, L., Sun, Y., Guan, S., Liu, B., Kong, L., The effect of hierarchical micro/nanosurface titanium implant on osseointegration in ovariectomized sheep (2011) Osteoporosis International: A Journal Established As Result of Cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 22, pp. 1907-1913; 
Frenkel, S.R., Simon, J., Alexander, H., Dennis, M., Ricci, J.L., Osseointegration on metallic implant surfaces: Effects of microgeometry and growth factor treatment (2002) Journal of Biomedical Materials Research, 63 (6), pp. 706-713. , DOI 10.1002/jbm.10408; 
Chehroudi, B., Ratkay, J., Brunette, D.M., The role of implant surface geometry on mineralization invivo and invitro - A transmission and scanning electron-microscopic study (1992) Cells and Materials, 2, pp. 89-104; 
Chehroudi, B., McDonnell, D., Brunette, D.M., The effects of micromachined surfaces on formation of bonelike tissue on subcutaneous implants as assessed by radiography and computer image processing (1997) Journal of Biomedical Materials Research, 34 (3), pp. 279-290. , DOI 10.1002/(SICI)1097-4636 (19970305)34:3<279:: AID-JBM2>3.0.CO;2- H; 
Laranjeira, M.S., Fernandes, M.H., Monteiro, F.J., Innovative macroporous granules of nanostructured-hydroxyapatite agglomerates: Bioactivity and osteoblast-like cell behaviour (2010) Journal of Biomedical Materials Research Part A, 95, pp. 891-900; 
Duran, A., Conde, A., Coedo, A.G., Dorado, T., Garcia, C., Cere, S., Sol-gel coatings for protection and bioactivation of metals used in orthopaedic devices (2004) Journal of Materials Chemistry, 14, pp. 2282-2290; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.M., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) Journal of Applied Biomaterials and Biomechanics: JABB, 2, pp. 74-80; 
Manuel, C.M., Ferraz, M.P., Monteiro, F.J., Synthesis of hydroxyapatite and tricalcium phosphate nanoparticles preliminary studies (2003) Bioceramics, 15 (2402), pp. 555-558; 
Garcia, C., Galliano, P., Cere, S., Electrochemical evaluation of resistance to localised corrosion of vitreous coatings containing particles applied on metallic substrates for biomedical applications (2003) Materials Letters, 57, pp. 1810-1814; 
Braet, F., De Zanger, R., Wisse, E., Drying cells for SEM, AFM and TEM by hexamethyldisilazane: A study on hepatic endothelial cells (1997) Journal of Microscopy, 186 (1), pp. 84-87; 
Teixeira, S., Fernandes, H., Leusink, A., Van Blitterswijk, C., Ferraz, M.P., Monteiro, F.J., De Boer, J., In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering (2010) Journal of Biomedical Materials Research Part A, 93, pp. 567-575; 
Teixeira, S., Fernandes, M.H., Ferraz, M.P., Monteiro, F.J., Proliferation and mineralization of bone marrow cells cultured on macroporous hydroxyapatite scaffolds functionalized with collagen type i for bone tissue regeneration (2010) Journal of Biomedical Materials Research Part A, 95, pp. 1-8; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) Journal of Materials Science: Materials in Medicine, 19 (2), pp. 855-859. , DOI 10.1007/s10856-007-3005-x; 
Teixeira, S., Monteiro, F.J., Ferraz, M.P., Vilar, R., Eugenio, S., Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies (2007) Journal of Biomedical Materials Research - Part A, 81 (4), pp. 920-929. , DOI 10.1002/jbm.a.31073; 
Teixeira, S., Yang, L., Dijkstra, P.J., Ferraz, M.P., Monteiro, F.J., Heparinized hydroxyapatite/collagen three-dimensional scaffolds for tissue engineering (2010) Journal of Materials Science Materials in Medicine, 21, pp. 2385-2392; 
Heinz, B., Kasaj, A., Teich, M., Jepsen, S., Clinical effects of nanocrystalline hydroxyapatite paste in the treatment of intrabony periodontal defects: A randomized controlled clinical study (2010) Clinical Oral Investigations, 14, pp. 525-531; 
Strietzel, F.P., Reichart, P.A., Graf, H.L., Lateral alveolar ridge augmentation using a synthetic nano-crystalline hydroxyapatite bone substitution material (Ostim): Preliminary clinical and histological results (2007) Clinical Oral Implants Research, 18, pp. 743-751; 
Pacifico, J., Endo, K., Morgan, S., Mulvaney, P., Superhydrophobic effects of self-assembled monolayers on micropatterned surfaces: 3-D arrays mimicking the Lotus Leaf (2006) Langmuir, 22 (26), pp. 11072-11076. , DOI 10.1021/la060925d; 
Tadanaga, K., Fujii, T., Matsuda, A., Minami, T., Tatsumisago, M., Micropatterning of SnO(2) thin films using hydrophobic-hydrophilic patterned surface (2004) Ceramics International, 30, pp. 1815-1817; 
Jung, Y.C., Bhushan, B., Contact angle, adhesion and friction properties of micro-and nanopatterned polymers for superhydrophobicity (2006) Nanotechnology, 17 (19), pp. 4970-4980. , DOI 10.1088/0957-4484/17/19/033, PII S0957448406276463, 033; 
Ribeiro, N., Sousa, S.R., Monteiro, F.J., Influence of crystallite size of nanophased hydroxyapatite on fibronectin and osteonectin adsorption and on MC3T3-E1 osteoblast adhesion and morphology (2010) Journal of Colloid and Interface Science, 351, pp. 398-406; 
Barthlott, W., Neinhuis, C., Purity of the sacred lotus, or escape from contamination in biological surfaces (1997) Planta, 202 (1), pp. 1-8. , DOI 10.1007/s004250050096; 
Katsikogianni, M., Missirlis, Y.F., Harris, L., Douglas, J., Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions (2004) European Cells and Materials, 8, pp. 37-57. , http://www.ecmjournal.org/journal/papers/vol008/pdf/v008a05.pdf; 
Mei, L., Busscher, H.J., Van Der Mei, H.C., Ren, Y., Influence of surface roughness on streptococcal adhesion forces to composite resins (2011) Dental Materials, 27, pp. 770-778; 
Hetrick, E.M., Schoenfisch, M.H., Reducing implant-related infections: Active release strategies (2006) Chemical Society Reviews, 35 (9), pp. 780-789. , DOI 10.1039/b515219b
},
	month = {2012///},
	pages = {1250 - 1260},
	abstract = {Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO                        2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 
                        2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 
                        2 surfaces with 0\%, 1\% and 5\% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 
                        2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. {\textcopyright} 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
},
	keywords = {Adhesion, AFM, Aggregates, Bacteria, Bacterial adhesion, Bioactive nanoparticles, Biological characterization, Biological materials, Biological response, Biomaterials, Cell culture, Contact angle, Dental prostheses, Flowcharting, FTIR/ATR, Guided tissue regeneration, Lithography, Mesenchymal stem cell, Micro patterning, Micro-texture, Microaggregates, Micropatterned, Micropatterned surface, Micropatterning, Nano-HA, Nano-hydroxyapatite, S. mutans, SEM/EDS, Silica, Silica thin films, Soft-lithography, Sol-gel, Sol-gel process, Sol-gels, Streptococcus mutans, Surface modification techniques, Surfaces, Thin films},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867841771\&partnerID=40\&md5=f33477063f16e99361687470c94d7289},
	author = {Carvalho, A. and Pelaez-Vargas, A. and Gallego-Perez, D. and Grenho, L. and Fernandes, M.H. and De Aza, A.H. and Ferraz, M.P. and Hansford, D.J. and Monteiro, F.J.}
}
@article {2194,
	title = {A model for educational simulation of the evolution of uterine contractions during labor},
	journal = {Computer Methods and Programs in BiomedicineComput. Methods Programs Biomed.},
	volume = {107},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: CMPBEdoi: 10.1016/j.cmpb.2011.09.016PubMed ID: 22047937Language of Original Document: EnglishCorrespondence Address: Bastos, L.F.; Campus da FEUP, Rua Dr. Roberto Frias, s/n, sala I305, 4200-465 Porto, Portugal; email: lbastos@fe.up.ptReferences: Curran, C.A., Intrapartum emergencies (2003) J. Obstet. Gynecol. Neonatal Nurs., 32 (6), pp. 802-813;Guise, J.-M., Anticipating and responding to obstetric emergencies (2007) Best Pract. Res. Clin. Obstet. Gynaecol., 21 (4), pp. 625-638; 
Draycott, T., Sibanda, T., Owen, L., Akande, V., Winter, C., Reading, S., Whitelaw, A., Does training in obstetric emergencies improve neonatal outcome? (2006) BJOG, 113 (2), pp. 177-182; 
Gardner, R., Walzer, T.B., Simon, R., Raemer, D.B., Obstetric simulation as a risk control strategy: course design and evaluation (2008) Simul. Healthc., 3 (2), pp. 119-127; 
Daniels, K., Lipman, S., Harney, K., Arafeh, J., Druzin, M., Use of simulation based team training for obstetric crises in resident education (2008) Simul. Healthc., 3 (3), pp. 154-160; 
Doyle, C., Angelotti, T., Diagnosis of an unsuspected maternal hemorrhage via fetal heart rate tracing (2004) J. Clin. Anesth., 16 (6), pp. 465-468; 
Odendaal, H.J., The frequency of uterine contractions in abruptio placentae (1976) S. Afr. Med. J., 50 (54), pp. 2129-2131; 
(2001) The Use of Electronic Fetal Monitoring: The Use and Interpretation of Cardiotocography in Intrapartum Fetal Surveillance, , RCOG,Royal-College-of-Obstetricians-and-Gynaecologists; 
Klavan, M., Laver, A.T., Boscola, M.A., (1977) Clinical Concepts of Fetal Heart Rate Monitoring, , Hewlett-Packard Waltham, Mass; 
Leveno, K.J., Cunningham, F.J., Gant, N.F., Alexander, J.M., Bloom, S.L., Casey, B.M., Dashe, J.S., Yost, N.P., (2003) Williams Manual of Obstetrics, , McGraw-Hill; 
Parer, J.T., (1997) Handbook of Fetal Heart Rate Monitoring, , W.B. Saunders Company; 
Bakker, P.C.A.M., van Geijn, H.P., Uterine activity: implications for the condition of the fetus (2008) J. Perinat. Med., 36 (1), pp. 30-37; 
Bakker, P.C.A.M., Van Rijswijk, S., van Geijn, H.P., Uterine activity monitoring during labor (2007) J. Perinat. Med., 35 (6), pp. 468-477; 
Dastur, A.E., Intrapartum fetal distress (2005) J. Obstet. Gynecol. India, 55 (2), pp. 115-117; 
Sadovsky, E., Rabinowitz, R., Freeman, A., Yarkoni, S., The relationship between fetal heart rate accelerations, fetal movements, and uterine contractions (1984) Am. J. Obstet. Gynecol., 149 (2), pp. 187-189; 
Arulkumaran, S., Chua, S., Ratnam, S.S., Symptoms and signs with scar rupture - value of uterine activity measurements (1992) Aust. N.Z. J. Obstet. Gynaecol., 32 (3), pp. 208-212; 
Beckley, S., Gee, H., Newton, J.R., Scar rupture in labour after previous lower uterine segment caesarean section: the role of uterine activity measurement (1991) Br. J. Obstet. Gynaecol., 98 (3), pp. 265-269; 
Burchell, H.J., Odendaal, H.J., de Wet, J.I., Badenhorst, P.N., Uterine contraction patterns in abruptio placentae (1985) S. Afr. Med. J., 67 (2), pp. 48-50; 
Phelan, J.P., Korst, L.M., Settles, D.K., Uterine activity patterns in uterine rupture: a case-control study (1998) Obstet. Gynecol., 92 (3), pp. 394-397; 
Rodriguez, M.H., Masaki, D.I., Phelan, J.P., Diaz, F.G., Uterine rupture: are intrauterine pressure catheters useful in the diagnosis? (1989) Am. J. Obstet. Gynecol., 161 (3), pp. 666-669; 
Andersen, H.F., Barclay, M.L., A computer-model of uterine contractions based on discrete contractile elements (1995) Obstet. Gynecol., 86 (1), pp. 108-111; 
Young, R.C., A computer model of uterine contractions based on action potential propagation and intercellular calcium waves (1997) Obstet. Gynecol., 89 (4), pp. 604-608; 
Vauge, C., Carbonne, B., Papiernik, E., Ferre, F., A mathematical model of uterine dynamics and its application to human parturition (2000) Acta Biotheor., 48 (2), pp. 95-105; 
Wray, S., Uterine contraction and physiological mechanisms of modulation (1993) Am. J. Physiol., 264 (1), pp. C1-C18; 
Wray, S., Kupittayanant, S., Shmygol, A., Smith, R.D., Burdyga, T., The physiological basis of uterine contractility: a short review (2001) Exp. Physiol., 86 (2), pp. 239-246; 
Bastos, L.F., Lobo, M.F., van-Meurs, W.L., Ayres-de-Campos, D., An intrauterine pressure generator for educational simulation of labor and delivery (2009) SESAM-ALASIC Winter Retreat; 
Harbert, G.M., Assessment of uterine contractility and activity (1992) Clin. Obstet. Gynecol., 35 (3), pp. 546-558; 
Matsuo, K., Scanlon, J.T., Atlas, R.O., Kopelman, J.N., Staircase sign: a newly described uterine contraction pattern seen in rupture of unscarred gravid uterus (2008) J. Obstet. Gynaecol. Res., 34 (1), pp. 100-104; 
Meniru, G.I., Brister, E., Nemunaitis-Keller, J., Gill, P., Krew, M., Hopkins, M.P., Spontaneous prolonged hypertonic uterine contractions (essential uterine hypertonus) and a possible infective etiology (2002) Arch. Gynecol. Obstet., 266 (4), pp. 238-240; 
Caspi, B., Lancet, M., Kessler, I., Sinusoidal pattern of uterine contractions in abruptio placentae (1980) Int. J. Gynaecol. Obstet., 17 (6), pp. 615-616; 
Eskes, T.K., Abruptio placentae. A "classic" dedicated to Elizabeth Ramsey (1997) Eur. J. Obstet. Gynecol. Reprod. Biol., 75 (1), pp. 63-70; 
Odendaal, H.J., Uterine contraction patterns in patients with severe abruptio placentae (1980) S. Afr. Med. J., 57 (22), pp. 908-910; 
Odendaal, H.J., Burchell, H., Raised uterine resting tone in patients with abruptio placentae (1985) Int. J. Gynaecol. Obstet., 23 (2), pp. 121-124; 
Sweeten, K.M., Graves, W.K., Athanassiou, A., Spontaneous rupture of the unscarred uterus (1995) Am. J. Obstet. Gynecol., 172 (6), pp. 1851-1855; 
Cobo, E., Quintero, C.A., Strada, G., Zambrana, M.A., Uterine behavior in abruptio placentae. I. Contraction patterns and their reactivity to oxytocin (1965) Am. J. Obstet. Gynecol., 93 (8), pp. 1151-1156; 
Eskes, T., Seelen, J., Stolte, L., Intrauterine pressure in placental abruption (1967) Ned. Tijdschr. Verloskd. Gynaecol., 67 (1), pp. 74-92; 
Beckmann, C.R., Byler, M., Jackson, K., Increasing baseline intrauterine pressure associated with impending spontaneous uterine rupture (1997) Int. J. Gynaecol. Obstet., 58 (2), pp. 239-240; 
Warrick, P.A., Hamilton, E.F., Precup, D., Kearney, R.E., Identification of the dynamic relationship between intrapartum uterine pressure and fetal heart rate for normal and hypoxic fetuses (2009) IEEE Trans. Biomed. Eng., 56 (6), pp. 1587-1597; 
Paul, M.J., Smeltzer, J.S., Relationship of measured external tocodynamometry with measured internal uterine activity (1991) Am. J. Perinatol., 8, pp. 417-420; 
Miles, A.M., Monga, M., Richeson, K.S., Correlation of external and internal monitoring of uterine activity in a cohort of term patients (2001) Am. J. Perinatol., 18, pp. 137-140
},
	month = {2012///},
	pages = {242 - 247},
	abstract = {Electronic fetal monitoring remains an important tool in labor ward settings, providing continuous information on fetal heart rate and maternal uterine contractions. A prompt detection of abnormalities in these signals is essential for the timely resolution of situations that may put both mother and fetus at risk. Uterine contraction signals provide information that is important to evaluate the onset and progress of labor, as well as the significance of certain fetal heart rate abnormalities. We present a model for educational simulation of the spontaneous evolution of uterine contractions during labor, which combines a previously published signal generator with literature-based pre-programmed scripts for educationally relevant scenarios. This model is an essential component of a high-fidelity simulator of intrapartum emergencies, aimed to improve the competency of healthcare providers. Real and simulated tracings were presented to three independent clinical experts who judged simulated signals to be indistinguishable or negligibly different from real tracings. {\textcopyright} 2011 Elsevier Ireland Ltd.},
	keywords = {article, clinical competence, computer memory, computer program, Computer simulation, Computer-Assisted Instruction, controlled study, Educational simulations, Essential component, Female, Fetal heart rate, Fetal monitoring, Health care, Health care providers, High-fidelity simulators, Humans, labor, Labor and delivery, medical education, Medical educational simulation, Models, Biological, Neonatal monitoring, Obstetric Labor Complications, Obstetrics, Pregnancy, Script-controlled simulation, signal detection, Signal generators, Simulated signals, User-Computer Interface, Uterine activities, Uterine activity, Uterine Contraction, Uterine Monitoring, uterus contraction},
	isbn = {01692607 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861989435\&partnerID=40\&md5=6b5099845e4592661e2f426170d9f8b9},
	author = {Bastos, L.F. and van Meurs, W. and Ayres-de-Campos, D.}
}
@booklet {2188,
	title = {Preparation and characterization of collagen-nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications},
	howpublished = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in PressCODEN: JBMRCdoi: 10.1002/jbm.a.34394Language of Original Document: EnglishCorrespondence Address: Rodrigues, S.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 41email: sandra88rodrigues@gmail.com},
	month = {2012///},
	abstract = {Recent efforts of bone repair focus on development of porous scaffolds for cell adhesion and proliferation. Collagen-nanohydroxyapatite (HA) scaffolds (70:30; 50:50; and 30:70 mass percentage) were produced by cryogelation technique using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide as crosslinking agents. A pure collagen scaffold was used as control. Morphology analysis revealed that all cryogels had highly porous structure with interconnective porosity and the nanoHA aggregates were randomly dispersed throughout the scaffold structure. Chemical analysis showed the presence of all major peaks related to collagen and HA in the biocomposites and indicated possible interaction between nanoHA aggregates and collagen molecules. Porosity analysis revealed an enhancement in the surface area as the nanoHA percentage increased in the collagen structure. The biocomposites showed improved mechanical properties as the nanoHA content increased in the scaffold. As expected, the swelling capacity decreased with the increase of nanoHA content. In vitro studies with osteoblasts cells showed that they were able to attach and spread in all cryogels surfaces. The presence of collagen-nanoHA biocomposites resulted in higher overall cellular proliferation compared to pure collagen scaffold. A statistically significant difference between collagen and collagen-nanoHA cryogels was observed after 21 day of cell culture. These innovative collagen-nanoHA cryogels could have potentially appealing application as scaffolds for bone regeneration. {\textcopyright} 2012 Wiley Periodicals, Inc.},
	keywords = {Biomaterials, Bone tissue engineering, Collagen, Cryogels, Nanohydroxyapatite},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84866486969\&partnerID=40\&md5=d00fe7ac9a8d6e95364631aa27ffa074},
	author = {Rodrigues, S.C. and Salgado, C.L. and Sahu, A. and Garcia, M.P. and Fernandes, M.H. and Monteiro, F.J.}
}
@conference {2200,
	title = {Proactive engineering},
	booktitle = {IEEE Global Engineering Education Conference, EDUCONIEEE Global Eng. Edu. Conf., EDUCON},
	series = {2012 IEEE Global Engineering Education Conference, EDUCON 2012},
	year = {2012},
	note = {Conference code: 91215Export Date: 21 November 2012Source: ScopusArt. No.: 6201104doi: 10.1109/EDUCON.2012.6201104Language of Original Document: EnglishCorrespondence Address: Duarte, C.; INESC TEC (Formerly INESC Porto), Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 378, 4200-465 Porto, PortugalReferences: Gheorghe, C., Covrig, M., Uncuta, S., Popescu, M., Does the implementation of the Bologna process in higher technical education require a new approach to motivate the actors involved in the educational process? (2011) 7th International Symposium on Advanced Topics in Electrical Engineering (ATEE{\textquoteright}2011), pp. 1-4. , May;(2011) Microelectronics Students{\textquoteright} Group, , http://usgroup.eu; 
(2011) BioStar, , http://biostar.fe.up.pt; 
Coke, P., Duarte, C., Cardoso, A., Tavares, V.G., De Oliveira, P.G., Network Infrastructure for academic IC CAD environments (2011) IEEE International Conference on Computer As A Tool (EUROCON{\textquoteright}2011), pp. 1-4. , Apr; 
Clarke, P., Students win training for being Cadence{\textquoteright}s fastest{\textquoteright} designers (2009) EE Times, , http://eetimes.eu, ID 217500055 May 14; 
Magalh{\~a}es, F., Oliveira, H.P., Campilho, A., (2010) SPD2010-Fingerprint Singular Points Detection Competition, , http://fe.up.pt/-spd2010/; 
Magalh{\~a}es, F., Oliveira, H.P., Matos, H., Campilho, A., (2011) HGC2011-Hand Geometric Points Detection Competition, , http://www.fe.up.pt/-hgc2011/; 
Duarte, C., Student group works on designs for a fully integrated wireless receiver (2009) IEEE Region 8 News, 2 (4), p. 9. , Dec; 
Dias, D., (2011) Computational Vision Applied to the Segmentation and Morphmetric Characterization of the Sciatic Nerve in Microscopic Images, , M.Sc. Thesis, Faculdade de Engenharia, Universidade do Porto, Jul; 
Martins, D., (2011) Biometric Recognition Based on the Texture Along Palmprint Principal Lines, , M.Sc. Thesis, Faculdade de Engenharia, Universidade do Porto, Jul; 
Matos, H., (2011) Hand Geometry Based Recognition System, , M.Sc. Thesis, Faculdade de Engenharia, Universidade do Porto, Jan; 
Soares, J., (2011) Uncalibrated Stereo Vision Applied to Breast Cancer Treatment Aesthetic Assessment, , M.Sc. Thesis, Faculdade de Engenharia, Universidade do Porto, Jul; 
Matos, H., Magalh{\~a}es, F., Oliveira, H.P., Cardoso, J.S., Hand geometry based recognition system-A new method with no constraints on image acquisition (2011) Proceedings of the 4th Meeting of Young Researchers of the University of Porto; 
Gaspar, D., Bre, L., Bishoff, N., Domingues, I., Mammograms image processing (2011) Proceedings of the 4th Meeting of Young Researchers of the University of Porto; 
Magalh{\~a}es, F., Oliveira, H.P., Campilho, A.C., A new method for the detection of singular points in fingerprint images (2009) Proceedings of the IEEE Workshop on Applications of Computer Vision, pp. 157-162; 
Moreira, I.C., Amaral, I., Domingues, I., Cardoso, A., Cardoso, M.J., Cardoso, J.S., INbreast: Toward a full-field digital mammographic database (2011) Academic Radiology; 
Oliveira, H.P., Magalhaes, A., Cardoso, M.J., Cardoso, J.S., An accurate and interpretable model for BCCT.core (2010) Proceedings of the 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, pp. 6158-6161; 
(2011) IJUP-Junior Reasearch at UP, , http://ijup.up.pt, University of Porto
},
	month = {2012///},
	address = {Marrakech},
	abstract = {This paper presents two initiatives run by groups of engineering students at the University of Porto: the Microelectronics Students{\textquoteright} Group and BioStar. These groups are student-led initiatives that promote different scientific fields through self-guided projects. Both experiences have proven to be very successful in increasing the undergraduate student{\textquoteright}s interest in science and technology. This work reports the activities, organization and main methodologies employed by these groups, which can be seen as successful approaches to enhance the technical curriculum of students. {\textcopyright} 2012 IEEE.},
	keywords = {Curricula, Engineering education, Microelectronics, Project based learning, project-based learning, Science and Technology, Scientific fields, student-led initiatives, Students, Undergraduate students},
	isbn = {21659559 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864143084\&partnerID=40\&md5=3b616738c3bc4fbca798784210235f95},
	author = {Duarte, C. and Oliveira, H.P. and Magalhaes, F. and Tavares, V.G. and Campilho, A.C. and De Oliveira, P.G.}
}
@article {2193,
	title = {Reciprocal induction of human dermal microvascular endothelial cells and human mesenchymal stem cells: Time-dependent profile in a co-culture system},
	journal = {Cell ProliferationCell Prolif.},
	volume = {45},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: CPROEdoi: 10.1111/j.1365-2184.2012.00822.xPubMed ID: 22607133Language of Original Document: EnglishCorrespondence Address: Laranjeira, M.S.; Departamento de Engenharia Metalurgicae de Materiais, Faculdade de Engenharia (FEUP), Universidade do Porto, Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: martalaranjeira@gmail.comMolecular Sequence Numbers: GENBANK: AB021221, NM_000088, NM_000552, NM_001200, NM_001795;Chemicals/CAS: DNA, 9007-49-2; F actin, 39409-31-9; alkaline phosphatase, 9001-78-9; calcein, 1461-15-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; DNA PrimersReferences: Santos, M., Reis, R., Vascularization in bone tissue engineering: physiology, current strategies, major hurdles and future challenges (2010) Macromol. Biosci., 10, pp. 12-20;Grellier, M., Granja, P.L., Fricain, J.-C., Bidarra, S.J., Renard, M., Bareille, R., The effect of the co-immobilization of human osteoprogenitors and endothelial cells within alginate microspheres on mineralization in a bone defect (2009) Biomaterials, 30, pp. 3271-3278; 
Unger, R.E., Sartoris, A., Peters, K., Motta, A., Migliaresi, C., Kunkel, M., Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials (2007) Biomaterials, 28, pp. 3965-3976; 
Santos, M.I., Pashkuleva, I., Alves, C.M., Gomes, M.E., Fuchs, S., Unger, R.E., Surface-modified 3D starch-based scaffold for improved endothelialization for bone tissue engineering (2009) J. Mater. Chem., 19, pp. 4029-4280; 
Fuchs, S., Jiang, X., Schmidt, H., Dohle, E., Ghanaati, S., Orth, C., Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells (2009) Biomaterials, 30, pp. 1329-1338; 
Santos, M.I., Unger, R.E., Sousa, R.A., Reis, R.L., Kirkpatrick, C.J., Crosstalk between osteoblasts and endothelial cells co-cultured on a polycaprolactone-starch scaffold and the in vitro development of vascularization (2009) Biomaterials, 30, pp. 4407-4415; 
Unger, R.E., Ghanaati, S., Orth, C., Sartoris, A., Barbeck, M., Halstenberg, S., The rapid anastomosis between prevascularized networks on silk fibroin scaffolds generated in vitro with cocultures of human microvascular endothelial and osteoblast cells and the host vasculature (2010) Biomaterials, 31, pp. 6959-6967; 
Stahl, A., Wenger, A., Weber, H., Stark, G.B., Augustin, H.G., Finkenzeller, G., Bi-directional cell contact-dependent regulation of gene expression between endothelial cells and osteoblasts in a three-dimensional spheroidal coculture model (2004) Biochem. Biophys. Res. Commun., 322, pp. 684-692; 
Yu, H., VandeVord, P.J., Mao, L., Matthew, H.W., Wooley, P.H., Yang, S.-Y., Improved tissue-engineered bone regeneration by endothelial cell mediated vascularization (2009) Biomaterials, 30, pp. 508-517; 
Amaral, I.F., Unger, R.E., Fuchs, S., Mendon{\c c}a, A.M., Sousa, S.R., Barbosa, M.A., Fibronectin-mediated endothelialisation of chitosan porous matrices (2009) Biomaterials, 30, pp. 5465-5475; 
Silva Marques, J.M., Gomes, P.S., Silva, M.A., Silv{\'e}rio Cabrita, A.M., Santos, J.D., Fernandes, M.H., Growth and phenotypic expression of human endothelial cells cultured on a glass-reinforced hydroxyapatite (2009) J. Mater. Sci. Mater. Med., 20, pp. 725-731; 
Hofmann, A., Ritz, U., Verrier, S., Eglin, D., Alini, M., Fuchs, S., The effect of human osteoblasts on proliferation and neo-vessel formation of human umbilical vein endothelial cells in a long-term 3D co-culture on polyurethane scaffolds (2008) Biomaterials, 29, pp. 4217-4226; 
Dwight, A.T., The Osteogenic-Angiogenic Interface: novel Insights into the Biology of Bone Formation and Fracture Repair (2008) Bone Biol. Struct., 6, pp. 67-71; 
Soker, S., Machado, M., Atala, A., Systems for therapeutic angiogenesis in tissue engineering (2000) World J. Urol., 18, pp. 10-18; 
Villars, F., Bordenave, L., Bareille, R., Am{\'e}d{\'e}e, J., Effect of human endothelial cells on human bone marrow stromal cell phenotype: role of VEGF? (2000) J. Cell. Biochem., 79, pp. 672-685; 
Lozito, T.P., Kuo, C.K., Taboas, J.M., Tuan, R.S., Human mesenchymal stem cells express vascular cell phenotypes upon interaction with endothelial cell matrix (2009) J. Cell. Biochem., 107, pp. 714-722; 
Saleh, F., Whyte, M., Genever, P., Effects of endothelial cells on human mesenchymal stem cell activity in a three-dimensional in vitro model (2011) Eur. Cells Mater., 22, pp. 242-257; 
Bajada, S., Mazakova, I., Richardson, J.B., Ashammakhi, N., Updates on stem cells and their applications in regenerative medicine (2008) J. Tissue Eng. Regen. Med., 2, pp. 169-183; 
Kolf, C.M., Cho, E., Tuan, R.S., Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation (2007) Arthritis Res. Ther., 9, p. 204; 
Lang, I., Pabst, M.A., Hiden, U., Blaschitz, A., Dohr, G., Hahn, T., Heterogeneity of microvascular endothelial cells isolated from human term placenta and macrovascular umbilical vein endothelial cells (2003) Eur. J. Cell Biol., 82, pp. 163-173; 
Ciardelli, G., Gentile, P., Chiono, V., Mattioli-Belmonte, M., Vozzi, G., Barbani, N., Enzymatically crosslinked porous composite matrices for bone tissue regeneration (2010) J. Biomed. Mater. Res. A, 92, pp. 137-151; 
Zhang, Y., Andrukhov, O., Bernerd, S., Matejka, M., Wieland, M., Rausch-Fan, X., Osteogenic properties of hydrophilic and hydrophobic titanium surfaces evaluated with osteoblast-like cells (MG63) in coculture with human umbilical vein endothelial cells(HUVEC) (2010) Dent. Mater., 400, pp. 284-292; 
Fuchs, S., Ghanaati, S., Orth, C., Barbeck, M., Kolbe, M., Hofmann, A., Contribution of outgrowth endothelial cells from human peripheral blood on in vivo vascularization of bone tissue engineered constructs based on starch polycaprolactone scaffolds (2009) Biomaterials, 30, pp. 526-534; 
Deb, S., Mandegaran, R., Di Silvio, L., A porous scaffold for bone tissue engineering 45S5 bioglass derived porous scaffolds for co-culturing osteoblasts and endothelial cells (2010) J. Mater. Sci. Mater. Med., 21, pp. 893-905; 
Aguirre, A., Planell, J.A., Engel, E., Dynamics of bone marrow-derived endothelial progenitor cell/mesenchymal stem cell interaction in co-culture and its implications in angiogenesis (2010) Biochem. Biophys. Res. Commun., 26, pp. 1043-1051; 
Grellier, M., Ferreira-Tojais, N., Bourget, C., Bareillke, R., Guillemot, F., Am{\'e}d{\'e}e, J., Role of vascular endothelial growth factor in the communication between human osteoprogenitors and endothelial cells (2009) J. Cell. Biochem., 106, pp. 390-398; 
Guillotin, B., Bareille, R., Bourget, C., Bordenave, L., Am{\'e}d{\'e}e, J., Interaction between human umbilical vein endothelial cells and human osteoprogenitors triggers pleiotropic effect that may support osteoblastic function (2008) Bone, 42, pp. 1080-1091; 
Grellier, M., Bordenave, L., Am{\'e}d{\'e}e, J., Cell-to-cell communication between osteogenic and endothelial lineages: implications for tissue engineering (2009) Trends Biotechnol., 27, pp. 562-571; 
Kirkpatrick, C.J., Peters, K., Hermanns, M.I., Bittinger, F., Krump-Konvalinkova, V., Fuchs, S., In vitro methodologies to evaluate biocompatibility: status quo and perspective (2005) ITBM-RBM, 26, pp. 192-199; 
Peters, K., Unger, R.E., Stumpf, S., Sch{\"a}fer, J., Tsaryk, R., Hoffmann, B., Cell type-specific aspects in biocompatibility testing: the intercellular contact in vitro as an indicator for endothelial cell compatibility (2008) J. Mater. Sci. Mater. Med., 19, pp. 1637-1644; 
Guillotin, B., Bourget, C., Remy-Zolgadri, M., Bareille, R., Fernandez, P., Conrad, V., Human primary endothelial cells stimulate human osteoprogenitor cell differentiation (2004) Cell. Physiol. Biochem., 14, pp. 325-332; 
Laranjeira, M.S., Fernandes, M.H., Monteiro, F.J., Innovative macroporous granules of nanostructured-hydroxyapatite agglomerates: bioactivity and osteoblast-like cell behaviour (2010) J. Biomed. Mater. Res. A, 95, pp. 891-900; 
Gomes, P.S., Fernandes, M.H., Effect of therapeutic levels of doxycycline and minocycline in the proliferation and differentiation of human bone marrow osteoblastic cells (2007) Arch. Oral Biol., 52, pp. 251-259; 
Zhang, Y., Schedle, A., Matejka, M., Rausch-Fan, X., Andrukhov, O., The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: an improved analysis using fluorescence-activated cell sorting (2010) Cell. Mol. Biol. Lett., 15, pp. 517-529; 
Shi, X., Ren, L., Tian, M., Yu, J., Huang, W., Du, C., In vivo and in vitro osteogenesis of stem cells induced by controlled release of drugs from microspherical scaffolds (2010) J. Mater. Chem., 20, pp. 9140-9148; 
Villars, F., Guillotion, B., Amedee, T., Dutoya, S., Bordenave, L., Bareille, R., Effect of HUVEC on human osteoprogenitor cell differentiation needs heterotypic gap junction communication (2002) Am. J. Physiol. Cell Physiol., 282, pp. C775-C785; 
Kanczler, J., Oreffo, R., Osteogenesis and angiogenesis: the potential for engineering bone (2008) Eur. Cell Mater., 15, pp. 100-114; 
Stiehler, M., Bunger, C., Baatrup, A., Lind, M., Kassem, M., Mygind, T., Effect of dynamic 3-D culture on proliferation, distribution, and osteogenic differentiation of human mesenchymal stem cells (2007) J. Biomed. Mater. Res. A, 89, pp. 96-107; 
Bouletreau, P., Warren, S., Spector, J., Peled, Z., Gerrets, R., Greenwald, J., Longaker, M., Hypoxia and VEGF up-regulate BMP-2 mRNA and protein expression in microvascular endothelial cells: implications for fracture healing (2002) Plast. Reconstr. Surg., 109, pp. 2384-2397; 
Deckers, M., van Bezooijen, R., van der Horst, G., Hoogendam, J., van der Bent, C., Papapoulos, S., L{\"o}wik, C., Bone morphogenetic proteins stimulate angiogenesis through osteoblast-derived vascular endothelial growth factor A (2002) Endocrinology, 143, pp. 1545-1553; 
Whelan, M.C., Senger, D.R., Collagen I initiates endothelial cell morphogenesis by inducing actin polymerization through suppression of cyclic AMP and protein kinase A (2003) J. Biol. Chem., 278, p. 327; 
Li, H., Daculsi, R., Grellier, M., Bareille, R., Bourget, C., Remy, M., Amedee, J., The role of vascular actors in two dimensional dialogue of human bone marrow stromal cell and endothelial cell for inducing self-assembled network (2011) PLoS ONE, 6, pp. e16767; 
Beyer, E.C., Paul, D.L., Goodenough, D.A., Connexin family of gap junction proteins (1990) J. Membr. Biol., 116, pp. 187-194; 
Li, H., Daculsi, R., Grellier, M., Bareille, R., Bourget, C., Amedee, J., Role of neural-cadherin in early osteoblastic differentiation of human bone marrow stromal cells cocultured with human umbilical vein endothelial cells (2010) Am. J. Physiol. Cell Physiol., 299, pp. C422-C430; 
Kyriakidou, K., Lucarini, G., Zizzi, A., Salvolini, E., Mattioli Belmonte, M., Mollica, F., Gloria, A., Ambrosio, L., Dynamic co-seeding of osteoblast and endothelial cells on 3D polycaprolactone scaffolds for enhanced bone tissue engineering (2008) J. Bioact. Compat. Polym., 23, p. 227; 
Saleh, F.A., Whyte, M., Ashton, P., Genever, P.G., Regulation of mesenchymal stem cell activity by endothelial cells (2010) Stem Cells Dev., 20, pp. 391-403; 
Lozito, T.P., Taboas, J.M., Kuo, C.K., Tuan, R.S., Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation (2009) J. Cell. Biochem., 107, pp. 706-713; 
Aubin, J.E., Heersche, J.N.M., Osteoprogenitor cell differentiation to mature bone-forming osteoblasts (2000) Drug Dev. Res., 49, pp. 206-215; 
Hoemann, C.D., El-Gabalawy, H., McKee, M.D., In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization (2009) Pathol. Biol. (Paris), 57, pp. 318-323; 
J{\o}rgensen, N.R., Henriksen, Z., S{\o}rensen, O.H., Civitelli, R., Dexamethasone, BMP-2, and 1,25-dihydroxyvitamin D enhance a more differentiated osteoblast phenotype: validation of an in vitro model for human bone marrow-derived primary osteoblasts (2004) Steroids, 69, pp. 219-226; 
Kaigler, D., Krebsbach, P.H., West, E.R., Horger, K., Huang, Y.C., Mooney, D.J., Endothelial cell modulation of bone marrow stromal cell osteogenic potential (2005) FASEB J., 19, pp. 665-667; 
Kempen, D.H.R., Lu, L., Heijink, A., Hefferan, T.E., Creemers, L.B., Maran, A., Yaszemski, M.J., Dhert, W.J.A., Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration (2009) Biomaterials, 30, pp. 2816-2825; 
M{\"u}ller, A.M., Hermanns, M.I., Skrzynski, C., Nesslinger, M., M{\"u}ller, K.-M., Kirkpatrick, C.J., Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro (2002) Exp. Mol. Pathol., 72, pp. 221-229; 
Bou{\"\i}s, D., Hospers, G., Meijer, C., Molema, G., Mulder, N., Endothelium in vitro: a review of human vascular endothelial cell lines for blood vessel-related research (2001) Angiogenesis, 4, pp. 91-102; 
Martin, T.A., Watkins, G., Lane, J., Jiang, W.G., Assessing microvessels and angiogenesis in human breast cancer, using VE-cadherin (2005) Histopathology, 46, pp. 422-430; 
Augustin, H.G., Kozian, D.H., Johnson, R.C., Differentiation of endothelial cells: analysis of the constitutive and activated endothelial cell phenotypes (1994) Bioessays, 16, pp. 901-906; 
Vestweber, D., Molecular mechanisms that control endothelial cell contacts (2000) J. Pathol., 190, pp. 281-291; 
Talavera-Adame, D., Ng, T.T., Gupta, A., Kurtovic, S., Wu, G.D., Dafoe, D.C., Characterization of microvascular endothelial cells isolated from the dermis of adult mouse tails (2011) Microvasc. Res., 82, pp. 97-104; 
Saharinen, P., Alitalo, K., The yin, the yang, and the Angiopoietin-1 (2011) J. Clin. Investig., 121, pp. 2157-2159; 
Bogdanovic, E., (2010) Regulation of Tie-2 by Angiopoietin-1 and Angiopoietin-2 in Endothelial Cells, , Toronto: University of Toronto; 
Suzuki, T., Miyamoto, T., Fujita, N., Ninomiya, K., Iwasaki, R., Toyama, Y., Suda, T., Osteoblast-specific Angiopoietin 1 overexpression increases bone mass (2007) Biochem. Biophys. Res. Commun., 362, pp. 1019-1025; 
Yuan, H.T., Khankin, E.V., Karumanchi, S.A., Parikh, S.M., Angiopoietin 2 is a partial agonist/antagonist of Tie2 signaling in the endothelium (2009) Mol. Cell. Biol., 29, p. 2011; 
Shilo, S., Roy, S., Khanna, S., Sen, C.K., Evidence for the involvement of miRNA in redox regulated angiogenic response of human microvascular endothelial cells (2008) Arterioscler. Thromb. Vasc. Biol., 28, pp. 471-477; 
Collin-Osdoby, P., Role of vascular endothelial cells in bone biology (1994) J. Cell. Biochem., 55, pp. 304-309
},
	month = {2012///},
	pages = {320 - 334},
	abstract = {Objectives: Angiogenesis is closely associated with osteogenesis where reciprocal interactions between endothelial and osteoblast cells play an important role in bone regeneration. For these reasons, the aim of this work was to develop a co-culture system to study in detail any time-dependent interactions between human mesenchymal stem cells (HMSC) and human dermal microvascular endothelial cells (HDMEC), co-cultured in a 2D system, for 35 days. Materials and methods: HMSC and HDMEC were co-cultured at a ratio of 1:4, respectively. Single-cell cultures were used as controls. Cell viability/proliferation was assessed using MTT, DNA quantification and calcein-AM assays. Cell morphology was monitored using confocal microscopy, and real time PCR was performed. Alkaline phosphatase activity and histochemical staining were evaluated. Matrix mineralization assays were also performed. Results: Cells were able to grow in characteristic patterns maintaining their viability and phenotype expression throughout culture time, compared to HMSC and HDMEC monocultures. HMSC differentiation seemed to be enhanced in the co-culture conditions, since it was observed an over expression of osteogenesis-related genes, and of ALP activity. Furthermore, presence of calcium phosphate deposits was also confirmed. Conclusions: This work reports in detail the interactions between HMSC and HDMEC in a long-term co-culture 2D system. Endothelial and mesenchymal stem cells cultured in the present co-culture conditions ensured proliferation and phenotype differentiation of cell types, osteogenesis stimulation and over-expression of angiogenesis-related genes, in the same culture system. It is believed that the present work can lead to significant developments for bone tissue regeneration and cell biology studies. {\textcopyright} 2012 Blackwell Publishing Ltd.},
	keywords = {alkaline phosphatase, article, Base Sequence, calcein, calcium phosphate, CD31 antigen, Cell culture, cell differentiation, cell growth, cell interaction, cell nucleus, cell population, cell proliferation, cell structure, cell viability, coculture, Coculture Techniques, confocal microscopy, controlled study, DNA, DNA determination, DNA Primers, Endothelium, Vascular, enzyme histochemistry, F actin, flow cytometry, gene overexpression, human, human cell, Humans, immunohistochemistry, in vitro study, Mesenchymal stem cell, Mesenchymal Stem Cells, Microscopy, Confocal, Microscopy, Electron, Scanning, microvascular endothelial cell, Microvessels, mineralization, nucleotide sequence, osteoblast, phenotype, process development, real time polymerase chain reaction, Real-Time Polymerase Chain Reaction, scanning electron microscope, single cell analysis, Skin},
	isbn = {09607722 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84862878215\&partnerID=40\&md5=4bbe59ef9f5c7b7424b78608e089c571},
	author = {Laranjeira, M.S. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {2184,
	title = {Regenerative medicine for the treatment of spinal cord injury: More than just promises?},
	journal = {Journal of Cellular and Molecular MedicineJ. Cell. Mol. Med.},
	volume = {16},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.1111/j.1582-4934.2012.01603.xLanguage of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Fitch, M.T., Silver, J., CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure (2008) Exp Neurol, 209, pp. 294-301;Ronaghi, M., Erceg, S., Moreno-Manzano, V., Challenges of stem cell therapy for spinal cord injury: human embryonic stem cells, endogenous neural stem cells, or induced pluripotent stem cells? (2010) Stem Cells, 28, pp. 93-99; 
Wyndaele, M., Wyndaele, J.J., Incidence, prevalence and epidemiology of spinal cord injury: what learns a worldwide literature survey? (2006) Spinal Cord, 44, pp. 523-529; 
Willerth, S.M., Sakiyama-Elbert, S.E., Cell therapy for spinal cord regeneration (2008) Adv Drug Deliv Rev, 60, pp. 263-276; 
Hulsebosch, C.E., Recent advances in pathophysiology and treatment of spinal cord injury (2002) Adv Physiol Educ, 26, pp. 238-255; 
Fawcett, J.W., Overcoming inhibition in the damaged spinal cord (2006) J Neurotrauma, 23, pp. 371-383; 
Garcia-Alias, G., Lin, R., Akrimi, S.F., Therapeutic time window for the application of chondroitinase ABC after spinal cord injury (2008) Exp Neurol, 210, pp. 331-338; 
Muir, E.M., Fyfe, I., Gardiner, S., Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells (2010) J Biotechnol, 145, pp. 103-110; 
Barnabe-Heider, F., Frisen, J., Stem cells for spinal cord repair (2008) Cell Stem Cell, 3, pp. 16-24; 
Bradbury, E.J., McMahon, S.B., Spinal cord repair strategies: why do they work? (2006) Nat Rev Neurosci, 7, pp. 644-653; 
Xu, X.M., Onifer, S.M., Transplantation-mediated strategies to promote axonal regeneration following spinal cord injury (2009) Respir Physiol Neurobiol, 169, pp. 171-182; 
Sykova, E., Homola, A., Mazanec, R., Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury (2006) Cell Transplant, 15, pp. 675-687; 
Wright, K.T., Masri, W.E., Osman, A., Concise review: bone marrow for the treatment of spinal cord injury: mechanisms and clinical applications (2011) Stem Cells, 29, pp. 169-178; 
Cizkova, D., Kakinohana, O., Kucharova, K., Functional recovery in rats with ischemic paraplegia after spinal grafting of human spinal stem cells (2007) Neuroscience, 147, pp. 546-560; 
Hofstetter, C.P., Schwarz, E.J., Hess, D., Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery (2002) Proc Natl Acad Sci USA, 99, pp. 2199-2204; 
Cizkova, D., Nagyova, M., Slovinska, L., Response of ependymal progenitors to spinal cord injury or enhanced physical activity in adult rat (2009) Cell Mol Neurobiol, 29, pp. 999-1013; 
Barnabe-Heider, F., Goritz, C., Sabelstrom, H., Origin of new glial cells in intact and injured adult spinal cord (2010) Cell Stem Cell, 7, pp. 470-482; 
Sykova, E., Jendelova, P., Magnetic resonance tracking of implanted adult and embryonic stem cells in injured brain and spinal cord (2005) Ann N Y Acad Sci, pp. 146-160; 
Kubinova, S., Sykova, E., Nanotechnology for treatment of stroke and spinal cord injury (2010) Nanomedicine-UK, 5, pp. 99-108; 
Madigan, N.N., McMahon, S., O{\textquoteright}Brien, T., Current tissue engineering and novel therapeutic approaches to axonal regeneration following spinal cord injury using polymer scaffolds (2009) Respir Physiol Neurobiol, 169, pp. 183-199; 
Blits, B., Boer, G.J., Verhaagen, J., Pharmacological, cell, and gene therapy strategies to promote spinal cord regeneration (2002) Cell Transplant, 11, pp. 593-613; 
Tetzlaff, W., Okon, E.B., Karimi-Abdolrezaee, S., A systematic review of cellular transplantation therapies for spinal cord injury (2011) J Neurotrauma, 28, pp. 1611-1682; 
Sykova, E., Jendelova, P., Urdzikova, L., Bone marrow stem cells and polymer hydrogels-two strategies for spinal cord injury repair (2006) Cell Mol Neurobiol, 26, pp. 1113-1129; 
Papastefanaki, F., Chen, J., Lavdas, A.A., Grafts of Schwann cells engineered to express PSA-NCAM promote functional recovery after spinal cord injury (2007) Brain, 130, pp. 2159-2174; 
Raisman, G., Repair of spinal cord injury by transplantation of olfactory ensheathing cells (2007) C R Biol, 330, pp. 557-560; 
Takahashi, K., Yamanaka, S., Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors (2006) Cell, 126, pp. 663-676; 
Okita, K., Ichisaka, T., Yamanaka, S., Generation of germline-competent induced pluripotent stem cells (2007) Nature, 448, pp. 313-317; 
Zhao, T., Zhang, Z.N., Rong, Z., Immunogenicity of induced pluripotent stem cells (2011) Nature, 474, pp. 212-215; 
Thomson, J.A., Odorico, J.S., Human embryonic stem cell and embryonic germ cell lines (2000) Trends Biotechnol, 18, pp. 53-57; 
Zeng, X., Rao, M.S., Human embryonic stem cells: long term stability, absence of senescence and a potential cell source for neural replacement (2007) Neuroscience, 145, pp. 1348-1358; 
Holubcova, Z., Matula, P., Sedlackova, M., Human embryonic stem cells suffer from centrosomal amplification (2011) Stem Cells, 29, pp. 46-56; 
Liu, S., Qu, Y., Stewart, T.J., Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation (2000) Proc Natl Acad Sci USA, 97, pp. 6126-6131; 
Kakinohana, O., Cizkova, D., Tomori, Z., Region-specific cell grafting into cervical and lumbar spinal cord in rat: a qualitative and quantitative stereological study (2004) Exp Neurol, 190, pp. 122-132; 
Lang, K.J., Rathjen, J., Vassilieva, S., Differentiation of embryonic stem cells to a neural fate: a route to re-building the nervous system? (2004) J Neurosci Res, 76, pp. 184-192; 
Carpenter, M.K., Inokuma, M.S., Denham, J., Enrichment of neurons and neural precursors from human embryonic stem cells (2001) Exp Neurol, 172, pp. 383-397; 
Li, X.J., Du, Z.W., Zarnowska, E.D., Specification of motoneurons from human embryonic stem cells (2005) Nat Biotechnol, 23, pp. 215-221; 
Keirstead, H.S., Nistor, G., Bernal, G., Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury (2005) J Neurosci, 25, pp. 4694-4705; 
Seminatore, C., Polentes, J., Ellman, D., The postischemic environment differentially impacts teratoma or tumor formation after transplantation of human embryonic stem cell-derived neural progenitors (2010) Stroke, 41, pp. 153-159; 
Faulkner, J., Keirstead, H.S., Human embryonic stem cell-derived oligodendrocyte progenitors for the treatment of spinal cord injury (2005) Transpl Immunol, 15, pp. 131-142; 
Nistor, G.I., Totoiu, M.O., Haque, N., Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation (2005) Glia, 49, pp. 385-396; 
Cloutier, F., Siegenthaler, M.M., Nistor, G., Transplantation of human embryonic stem cell-derived oligodendrocyte progenitors into rat spinal cord injuries does not cause harm (2006) Regen Med, 1, pp. 469-479; 
Altman, J., Are new neurons formed in the brains of adult mammals? (1962) Science, 135, pp. 1127-1128; 
Gage, F.H., Mammalian neural stem cells (2000) Science, 287, pp. 1433-1438; 
Goldman, S., Stem and progenitor cell-based therapy of the human central nervous system (2005) Nat Biotechnol, 23, pp. 862-871; 
Horky, L.L., Galimi, F., Gage, F.H., Fate of endogenous stem/progenitor cells following spinal cord injury (2006) J Comp Neurol, 498, pp. 525-538; 
Setoguchi, T., Nakashima, K., Takizawa, T., Treatment of spinal cord injury by transplantation of fetal neural precursor cells engineered to express BMP inhibitor (2004) Exp Neurol, 189, pp. 33-44; 
Cizkova, D., Cizek, M., Nagyova, M., Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting (2009) J Neurosci Meth, 184, pp. 88-94; 
Lu, P., Jones, L.L., Snyder, E.Y., Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury (2003) Exp Neurol, 181, pp. 115-129; 
Ishii, K., Nakamura, M., Dai, H., Neutralization of ciliary neurotrophic factor reduces astrocyte production from transplanted neural stem cells and promotes regeneration of corticospinal tract fibers in spinal cord injury (2006) J Neurosci Res, 84, pp. 1669-1681; 
Tarasenko, Y.I., Gao, J., Nie, L., Human fetal neural stem cells grafted into contusion-injured rat spinal cords improve behavior (2007) J Neurosci Res, 85, pp. 47-57; 
Cummings, B.J., Uchida, N., Tamaki, S.J., Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice (2005) Proc Natl Acad Sci USA, 102, pp. 14069-14074; 
Marsala, M., Kakinohana, O., Yaksh, T.L., Spinal implantation of hNT neurons and neuronal precursors: graft survival and functional effects in rats with ischemic spastic paraplegia (2004) Eur J Neurosci, 20, pp. 2401-2414; 
Wu, R., Wang, H., Xia, X., Nerve injection of viral vectors efficiently transfers transgenes into motor neurons and delivers RNAi therapy against ALS (2009) Antioxid Redox Signal, 11, pp. 1523-1534; 
Romanyuk, N., Amemori, T., Turnovcova, K., (2011), Using human fetal neural stem cells or human induced pluripotent stem cell-derived neural precursors for the treatment of experimental spinal cord injury. 8th IBRO World Congress of Neuroscience. Florence, ItalyHooshmand, M.J., Sontag, C.J., Uchida, N., Analysis of host-mediated repair mechanisms after human CNS-stem cell transplantation for spinal cord injury: correlation of engraftment with recovery (2009) PLoS ONE, 4, pp. e5871; 
Hofstetter, C.P., Holmstrom, N.A., Lilja, J.A., Allodynia limits the usefulness of intraspinal neural stem cell grafts; directed differentiation improves outcome (2005) Nature Neurosci, 8, pp. 346-353; 
Mukhida, K., Mendez, I., McLeod, M., Spinal GABAergic transplants attenuate mechanical allodynia in a rat model of neuropathic pain (2007) Stem Cells, 25, pp. 2874-2885; 
Takami, T., Oudega, M., Bates, M.L., Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord (2002) J Neurosci, 22, pp. 6670-6681; 
Pinzon, A., Calancie, B., Oudega, M., Conduction of impulses by axons regenerated in a Schwann cell graft in the transected adult rat thoracic spinal cord (2001) J Neurosci Res, 64, pp. 533-541; 
Guest, J.D., Hiester, E.D., Bunge, R.P., Demyelination and Schwann cell responses adjacent to injury epicenter cavities following chronic human spinal cord injury (2005) Exp Neurol, 192, pp. 384-393; 
Pearse, D.D., Sanchez, A.R., Pereira, F.C., Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery (2007) Glia, 55, pp. 976-1000; 
Bunge, M.B., Novel combination strategies to repair the injured mammalian spinal cord (2008) J Spinal Cord Med, 31, pp. 262-269; 
Lu, P., Tuszynski, M.H., Growth factors and combinatorial therapies for CNS regeneration (2008) Exp Neurol, 209, pp. 313-320; 
Afshari, F.T., Kwok, J.C., Fawcett, J.W., Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling (2010) J Neurosci, 30, pp. 4246-4255; 
Mackay-Sim, A., Olfactory ensheathing cells and spinal cord repair (2005) Keio J Med, 54, pp. 8-14; 
Feron, F., Perry, C., Cochrane, J., Autologous olfactory ensheathing cell transplantation in human spinal cord injury (2005) Brain, 128, pp. 2951-2960; 
Lima, C., Pratas-Vital, J., Escada, P., Olfactory mucosa autografts in human spinal cord injury: a pilot clinical study (2006) J Spinal Cord Med, 29, pp. 191-203; 
Mackay-Sim, A., Feron, F., Cochrane, J., Autologous olfactory ensheathing cell transplantation in human paraplegia: a 3-year clinical trial (2008) Brain, 131, pp. 2376-2386; 
Fouad, K., Schnell, L., Bunge, M.B., Combining Schwann cell bridges and olfactory-ensheathing glia grafts with chondroitinase promotes locomotor recovery after complete transection of the spinal cord (2005) J Neurosci, 25, pp. 1169-1178; 
Amemori, T., Jendelova, P., Ruzickova, K., Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat (2010) Cytotherapy, 12, pp. 212-225; 
Salem, H.K., Thiemermann, C., Mesenchymal stromal cells: current understanding and clinical status (2010) Stem Cells, 28, pp. 585-596; 
Cizkova, D., Rosocha, J., Vanicky, I., Transplants of human mesenchymal stem cells improve functional recovery after spinal cord injury in the rat (2006) Cell Mol Neurobiol, 26, pp. 1167-1180; 
Sykova, E., Jendelova, P., Migration, fate and in vivo imaging of adult stem cells in the CNS (2007) Cell Death Differ, 14, pp. 1336-1342; 
Urdzikova, L., Jendelova, P., Glogarova, K., Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats (2006) J Neurotrauma, 23, pp. 1379-1391; 
Peran, M., Marchal, J.A., Rodriguez-Serrano, F., Transdifferentiation: why and how? (2011) Cell Biol Int, 35, pp. 373-379; 
Lambert, A.P.F., Zandonai, A.F., Bonatto, D., Differentiation of human adipose-derived adult stem cells into neuronal tissue: Does it work? (2009) Differentiation, 77, pp. 221-228; 
Uccelli, A., Benvenuto, F., Laroni, A., Neuroprotective features of mesenchymal stem cells (2011) Best Pract Res Cl Ha, 24, pp. 59-64; 
Geffner, L.F., Santacruz, P., Izurieta, M., Administration of autologous bone marrow stem cells into spinal cord injury patients via multiple routes is safe and improves their quality of life: comprehensive case studies (2008) Cell Transplant, 17, pp. 1277-1293; 
Park, H.C., Shim, Y.S., Ha, Y., Treatment of complete spinal cord injury patients by autologous bone marrow cell transplantation and administration of granulocyte-macrophage colony stimulating factor (2005) Tissue Eng, 11, pp. 913-922; 
Yoon, S.H., Shim, Y.S., Park, Y.H., Complete spinal cord injury treatment using autologous bone marrow cell transplantation and bone marrow stimulation with granulocyte macrophage-colony stimulating factor: Phase I/II clinical trial (2007) Stem Cells, 25, pp. 2066-2073; 
Callera, F., do Nascimento, R.X., Delivery of autologous bone marrow precursor cells into the spinal cord via lumbar puncture technique in patients with spinal cord injury: a preliminary safety study (2006) Exp Hematol, 34, pp. 130-131; 
Deda, H., Inci, M.C., Kurekci, A.E., Treatment of chronic spinal cord injured patients with autologous bone marrow-derived hematopoietic stem cell transplantation: 1-year follow-up (2008) Cytotherapy, 10, pp. 565-574; 
Saito, F., Nakatani, T., Iwase, M., Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation: the first clinical trial case report (2008) J Trauma, 64, pp. 53-59; 
Pal, R., Venkataramana, N.K., Bansal, A., Ex vivo-expanded autologous bone marrow-derived mesenchymal stromal cells in human spinal cord injury/paraplegia: a pilot clinical study (2009) Cytotherapy, 11, pp. 897-911; 
Mason, M.R., Ehlert, E.M., Eggers, R., Comparison of AAV serotypes for gene delivery to dorsal root ganglion neurons (2010) Mol Ther, 18, pp. 715-724; 
Petruska, J.C., Kitay, B., Boyce, V.S., Intramuscular AAV delivery of NT-3 alters synaptic transmission to motoneurons in adult rats (2010) Eur J Neurosci, 32, pp. 997-1005; 
Tuszynski, M.H., Nerve growth factor gene delivery: animal models to clinical trials (2007) Dev Neurobiol, 67, pp. 1204-1215; 
Blits, B., Derks, S., Twisk, J., Adeno-associated viral vector (AAV)-mediated gene transfer in the red nucleus of the adult rat brain: comparative analysis of the transduction properties of seven AAV serotypes and lentiviral vectors (2010) J Neurosci Meth, 185, pp. 257-263; 
Aalbers, C.J., Tak, P.P., Vervoordeldonk, M.J., Advancements in adeno-associated viral gene therapy approaches: exploring a new horizon (2011) F1000 Med Reports, 3, p. 17; 
Straley, K.S., Foo, C.W.P., Heilshorn, S.C., Biomaterial design strategies for the treatment of spinal cord injuries (2010) J Neurotrauma, 27, pp. 1-19; 
Hejcl, A., Lesny, P., Pradny, M., Biocompatible hydrogels in spinal cord injury repair (2008) Physiol Res, 57, pp. S121-S132; 
Friedman, J.A., Windebank, A.J., Moore, M.J., Biodegradable polymer grafts for surgical repair of the injured spinal cord (2002) Neurosurgery, 51, pp. 742-752; 
De Laporte, L., Lei Yan, A., Shea, L.D., Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury (2009) Biomaterials, 30, pp. 2361-2368; 
Wong, D.Y., Leveque, J.C., Brumblay, H., Macro-architectures in spinal cord scaffold implants influence regeneration (2008) J Neurotrauma, 25, pp. 1027-1037; 
P{\^e}go, A.P., Poot, A.A., Grijpma, D.W., Copolymers of trimethylene carbonate and epsilon-caprolactone for porous nerve guides: Synthesis and properties (2001) J Biomat Sci-Polym E, 12, pp. 35-53; 
Tsai, E.C., Dalton, P.D., Shoichet, M.S., Synthetic hydrogel guidance channels facilitate regeneration of adult rat brainstem motor axons after complete spinal cord transection (2004) J Neurotrauma, 21, pp. 789-804; 
Woerly, S., Fort, S., Pignot-Paintrand, I., Development of a sialic acid-containing hydrogel of poly[N-(2-hydroxypropyl) methacrylamide]: characterization and implantation study (2008) Biomacromolecules, 9, pp. 2329-2337; 
Collazos-Castro, J.E., Polo, J.L., Hernandez-Labrado, G.R., Bioelectrochemical control of neural cell development on conducting polymers (2010) Biomaterials, 31, pp. 9244-9255; 
Ciardelli, G., Chiono, V., Materials for peripheral nerve regeneration (2006) Macromol Biosci, 6, pp. 13-26; 
Yoshii, S., Ito, S., Shima, M., Functional restoration of rabbit spinal cord using collagen-filament scaffold (2009) J Tissue Eng Regen M, 3, pp. 19-25; 
Taylor, S.J., McDonald III, J.W., Sakiyama-Elbert, S.E., Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury (2004) J Control Release, 98, pp. 281-294; 
P{\^e}go, A.P., Mar, F.M., Rocha, D.N., Scaffold-driven regenerative therapy for the spinal cord injury - biomimeting neurogenesis in the CNS (2011) Histol Histopathol, 26, p. 289; 
Wei, Y.T., He, Y., Xu, C.L., Hyaluronic acid hydrogel modified with nogo-66 receptor antibody and poly-L-lysine to promote axon regrowth after spinal cord injury (2010) J Biomed Mater Res B, 95, pp. 110-117; 
Park, J., Lim, E., Back, S., Nerve regeneration following spinal cord injury using matrix metalloproteinase-sensitive, hyaluronic acid-based biomimetic hydrogel scaffold containing brain-derived neurotrophic factor (2010) J Biomed Mater Res A, 93, pp. 1091-1099; 
Gros, T., Sakamoto, J.S., Blesch, A., Regeneration of long-tract axons through sites of spinal cord injury using templated agarose scaffolds (2010) Biomaterials, 31, pp. 6719-6729; 
Prang, P., Muller, R., Eljaouhari, A., The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels (2006) Biomaterials, 27, pp. 3560-3569; 
Bozkurt, G., Mothe, A.J., Zahir, T., Chitosan channels containing spinal cord-derived stem/progenitor cells for repair of subacute spinal cord injury in the rat (2010) Neurosurgery, 67, pp. 1733-1744; 
Shen, Y., Qian, Y., Zhang, H., Guidance of olfactory ensheathing cell growth and migration on electrospun silk fibroin scaffolds (2010) Cell Transplant, 19, pp. 147-157; 
Novikova, L.N., Pettersson, J., Brohlin, M., Biodegradable poly-beta-hydroxybutyrate scaffold seeded with Schwann cells to promote spinal cord repair (2008) Biomaterials, 29, pp. 1198-1206; 
Bhattarai, N., Li, Z., Gunn, J., Natural-synthetic polyblend nanofibers for biomedical applications (2009) Adv Mater, 21, pp. 2792-2797; 
Hejcl, A., Lesny, P., Pradny, M., Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 6: 3D hydrogels with positive and negative surface charges and polyelectrolyte complexes in spinal cord injury repair (2009) J Mater Sci Mater Med, 20, pp. 1571-1577; 
Kubinova, S., Horak, D., Sykova, E., Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering (2009) Biomaterials, 30, pp. 4601-4609; 
Kubinova, S., Horak, D., Hejcl, A., Highly superporous cholesterol-modified poly(2-hydroxyethyl methacrylate) scaffolds for spinal cord injury repair (2011) J Biomed Mater Res A, 99, pp. 618-629; 
Gunn, J.W., Turner, S.D., Mann, B.K., Adhesive and mechanical properties of hydrogels influence neurite extension (2005) J Biomed Mater Res A, 72, pp. 91-97; 
Kubinova, S., Horak, D., Kozubenko, N., The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors (2010) Biomaterials, 31, pp. 5966-5975; 
Cui, H., Webber, M.J., Stupp, S.I., Self-assembly of peptide amphiphiles: from molecules to nanostructures to biomaterials (2010) Biopolymers, 94, pp. 1-18; 
Tysseling-Mattiace, V.M., Sahni, V., Niece, K.L., Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury (2008) J Neurosci, 28, pp. 3814-3823; 
Bellamkonda, R., Ranieri, J.P., Bouche, N., Hydrogel-based three-dimensional matrix for neural cells (1995) J Biomed Mater Res, 29, pp. 663-671; 
Wang, M., Zhai, P., Chen, X., Bioengineered scaffolds for spinal cord repair (2011) Tissue Eng Pt B-Rev, 17, pp. 177-194; 
Stokols, S., Sakamoto, J., Breckon, C., Templated agarose scaffolds support linear axonal regeneration (2006) Tissue Eng, 12, pp. 2777-2787; 
Stokols, S., Tuszynski, M.H., Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury (2006) Biomaterials, 27, pp. 443-451; 
Moore, M.J., Friedman, J.A., Lewellyn, E.B., Multiple-channel scaffolds to promote spinal cord axon regeneration (2006) Biomaterials, 27, pp. 419-429; 
Wang, H.B., Mullins, M.E., Cregg, J.M., Creation of highly aligned electrospun poly-L-lactic acid fibers for nerve regeneration applications (2009) J Neural Eng, 6, p. 016001; 
Wang, H.B., Mullins, M.E., Cregg, J.M., Varying the diameter of aligned electrospun fibers alters neurite outgrowth and Schwann cell migration (2010) Acta Biomater, 6, pp. 2970-2978; 
Xie, J., MacEwan, M.R., Li, X., Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties (2009) ACS Nano, 3, pp. 1151-1159; 
Hurtado, A., Cregg, J.M., Wang, H.B., Robust CNS regeneration after complete spinal cord transection using aligned poly-l-lactic acid microfibers (2011) Biomaterials, 32, pp. 6068-6079; 
Guo, J., Su, H., Zeng, Y., Reknitting the injured spinal cord by self-assembling peptide nanofiber scaffold (2007) Nanomedicine, 3, pp. 311-321; 
Johnson, P.J., Tatara, A., McCreedy, D.A., Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI (2010) Soft Matter, 6, pp. 5127-5137; 
Olson, H.E., Rooney, G.E., Gross, L., Neural stem cell- and Schwann cell-loaded biodegradable polymer scaffolds support axonal regeneration in the transected spinal cord (2009) Tissue Eng Pt A, 15, pp. 1797-1805; 
Hurtado, A., Moon, L.D., Maquet, V., Poly (D, L-lactic acid) macroporous guidance scaffolds seeded with Schwann cells genetically modified to secrete a bi-functional neurotrophin implanted in the completely transected adult rat thoracic spinal cord (2006) Biomaterials, 27, pp. 430-442; 
Woerly, S., Pinet, E., de Robertis, L., Spinal cord repair with PHPMA hydrogel containing RGD peptides (NeuroGel) (2001) Biomaterials, 22, pp. 1095-1111; 
Loh, N.K., Woerly, S., Bunt, S.M., The regrowth of axons within tissue defects in the CNS is promoted by implanted hydrogel matrices that contain BDNF and CNTF producing fibroblasts (2001) Exp Neurol, 170, pp. 72-84; 
Hejcl, A., Sedy, J., Kapcalova, M., HPMA-RGD hydrogels seeded with mesenchymal stem cells improve functional outcome in chronic spinal cord injury (2010) Stem Cells Dev, 19, pp. 1535-1546; 
Willerth, S.M., Sakiyama-Elbert, S.E., Approaches to neural tissue engineering using scaffolds for drug delivery (2007) Adv Drug Deliv Rev, 59, pp. 325-338; 
Jimenez Hamann, M.C., Tator, C.H., Shoichet, M.S., Injectable intrathecal delivery system for localized administration of EGF and FGF-2 to the injured rat spinal cord (2005) Exp Neurol, 194, pp. 106-119; 
Jain, A., Kim, Y.T., McKeon, R.J., In situ gelling hydrogels for conformal repair of spinal cord defects, and local delivery of BDNF after spinal cord injury (2006) Biomaterials, 27, pp. 497-504; 
Piantino, J., Burdick, J.A., Goldberg, D., An injectable, biodegradable hydrogel for trophic factor delivery enhances axonal rewiring and improves performance after spinal cord injury (2006) Exp Neurol, 201, pp. 359-367; 
Hendriks, W.T., Ruitenberg, M.J., Blits, B., Viral vector-mediated gene transfer of neurotrophins to promote regeneration of the injured spinal cord (2004) Prog Brain Res, 146, pp. 451-476; 
Abdellatif, A.A., Pelt, J.L., Benton, R.L., Gene delivery to the spinal cord: comparison between lentiviral, adenoviral, and retroviral vector delivery systems (2006) J Neurosci Res, 84, pp. 553-567; 
Blits, B., Bunge, M.B., Direct gene therapy for repair of the spinal cord (2006) J Neurotrauma, 23, pp. 508-520; 
Oliveira, H., Fernandez, R., Pires, L.R., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c (2010) J Control Release, 143, pp. 350-358; 
Oliveira, H., Pires, L.R., Fernandez, R., Chitosan-based gene delivery vectors targeted to the peripheral nervous system (2010) J Biomed Mater Res A, 95, pp. 801-810; 
Whitehead, K.A., Langer, R., Anderson, D.G., Knocking down barriers: advances in siRNA delivery (2009) Nat Rev Drug Discov, 8, pp. 129-138; 
Mittnacht, U., Hartmann, H., Hein, S., Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth (2010) Nano Lett, 10, pp. 3933-3939; 
Richardson, P.M., McGuinness, U.M., Aguayo, A.J., Axons from CNS neurons regenerate into PNS grafts (1980) Nature, 284, pp. 264-265; 
David, S., Aguayo, A.J., Axonal elongation into peripheral nervous system "bridges" after central nervous system injury in adult rats (1981) Science, 214, pp. 931-933; 
Schweigreiter, R., The natural history of the myelin-derived nerve growth inhibitor Nogo-A (2008) Neuron Glia Biol, 4, pp. 83-89; 
Vargas, M.E., Barres, B.A., Why is Wallerian degeneration in the CNS so slow? (2007) Annu Rev Neurosci, 30, pp. 153-179; 
Neumann, S., Woolf, C.J., Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury (1999) Neuron, 23, pp. 83-91; 
Ylera, B., Erturk, A., Hellal, F., Chronically CNS-injured adult sensory neurons gain regenerative competence upon a lesion of their peripheral axon (2009) Curr Biol, 19, pp. 930-936; 
Qiu, J., Cai, D., Dai, H., Spinal axon regeneration induced by elevation of cyclic AMP (2002) Neuron, 34, pp. 895-903; 
Jacob, J.M., Croes, S.A., Acceleration of axonal outgrowth in motor axons from mature and old F344 rats after a conditioning lesion (1998) Exp Neurol, 152, pp. 231-237; 
McQuarrie, I.G., Grafstein, B., Effect of a conditioning lesion on optic nerve regeneration in goldfish (1981) Brain Res, 216, pp. 253-264; 
Neumann, S., Bradke, F., Tessier-Lavigne, M., Regeneration of sensory axons within the injured spinal cord induced by intraganglionic cAMP elevation (2002) Neuron, 34, pp. 885-893; 
Zou, H., Ho, C., Wong, K., Axotomy-induced Smad1 activation promotes axonal growth in adult sensory neurons (2009) J Neurosci, 29, pp. 7116-7123; 
Seijffers, R., Mills, C.D., Woolf, C.J., ATF3 increases the intrinsic growth state of DRG neurons to enhance peripheral nerve regeneration (2007) J Neurosci, 27, pp. 7911-7920; 
Cafferty, W.B., Gardiner, N.J., Gavazzi, I., Leukemia inhibitory factor determines the growth status of injured adult sensory neurons (2001) J Neurosci, 21, pp. 7161-7170; 
Cafferty, W.B., Gardiner, N.J., Das, P., Conditioning injury-induced spinal axon regeneration fails in interleukin-6 knock-out mice (2004) J Neurosci, 24, pp. 4432-4443; 
Cao, Z., Gao, Y., Bryson, J.B., The cytokine interleukin-6 is sufficient but not necessary to mimic the peripheral conditioning lesion effect on axonal growth (2006) J Neurosci, 26, pp. 5565-5573; 
Minor, K., Phillips, J., Seeds, N.W., Tissue plasminogen activator promotes axonal outgrowth on CNS myelin after conditioned injury (2009) J Neurochem, 109, pp. 706-715; 
Pearse, D.D., Pereira, F.C., Marcillo, A.E., cAMP and Schwann cells promote axonal growth and functional recovery after spinal cord injury (2004) Nat Med, 10, pp. 610-616; 
Nikulina, E., Tidwell, J.L., Dai, H.N., The phosphodiesterase inhibitor rolipram delivered after a spinal cord lesion promotes axonal regeneration and functional recovery (2004) Proc Natl Acad Sci USA, 101, pp. 8786-8790; 
Cai, D., Deng, K., Mellado, W., Arginase I and polyamines act downstream from cyclic AMP in overcoming inhibition of axonal growth MAG and myelin in vitro (2002) Neuron, 35, pp. 711-719; 
Ma, T.C., Campana, A., Lange, P.S., A large-scale chemical screen for regulators of the arginase 1 promoter identifies the soy isoflavone daidzeinas a clinically approved small molecule that can promote neuronal protection or regeneration via a cAMP-independent pathway (2010) J Neurosci, 30, pp. 739-748; 
Bregman, B.S., Kunkel-Bagden, E., Schnell, L., Recovery from spinal cord injury mediated by antibodies to neurite growth inhibitors (1995) Nature, 378, pp. 498-501; 
Bradbury, E.J., Moon, L.D., Popat, R.J., Chondroitinase ABC promotes functional recovery after spinal cord injury (2002) Nature, 416, pp. 636-640; 
Bareyre, F.M., Kerschensteiner, M., Raineteau, O., The injured spinal cord spontaneously forms a new intraspinal circuit in adult rats (2004) Nature Neurosci, 7, pp. 269-277; 
Saberi, H., Firouzi, M., Habibi, Z., Safety of intramedullary Schwann cell transplantation for postrehabilitation spinal cord injuries: 2-year follow-up of 33 cases (2011) J Neurosurg Spine, 15, pp. 515-525; 
Fukushima, K., Enomoto, M., Tomizawa, S., The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord (2008) J Med Dent Sci, 55, pp. 71-79; 
Madaghiele, M., Sannino, A., Yannas, I.V., Collagen-based matrices with axially oriented pores (2008) J Biomed Mater Res A, 85, pp. 757-767; 
Liang, W., Han, Q., Jin, W., The promotion of neurological recovery in the rat spinal cord crushed injury model by collagen-binding BDNF (2010) Biomaterials, 31, pp. 8634-8641; 
Liu, T., Houle, J.D., Xu, J., Nanofibrous Collagen Nerve Conduits for Spinal Cord Repair (2012) Tissue Eng Pt A, 18, pp. 1057-1066; 
Khaing, Z.Z., Milman, B.D., Vanscoy, J.E., High molecular weight hyaluronic acid limits astrocyte activation and scar formation after spinal cord injury (2011) J Neural Eng, 8, p. 046033; 
Mehrotra, S., Lynam, D., Maloney, R., Time Controlled Protein Release from Layer-by-Layer Assembled Multilayer Functionalized Agarose Hydrogels (2010) Adv Funct Mater, 20, pp. 247-258; 
Kataoka, K., Suzuki, Y., Kitada, M., Alginate enhances elongation of early regenerating axons in spinal cord of young rats (2004) Tissue Eng, 10, pp. 493-504; 
Chen, X., Yang, Y., Yao, J., Bone marrow stromal cells-loaded chitosan conduits promote repair of complete transection injury in rat spinal cord (2011) J Mater Sci Mater Med, 22, pp. 2347-2356; 
Kim, H., Tator, C.H., Shoichet, M.S., Chitosan implants in the rat spinal cord: biocompatibility and biodegradation (2011) J Biomed Mater Res A, 97, pp. 395-404; 
Ding, C., Guo, X., Cheng, B., Fabrication and radial compressive properties of the biodegradable woven regeneration conduits for peripheral nerve repair (2011) Adv Mat Res, 332-334, pp. 1481-1484; 
Suwantong, O., Waleetorncheepsawat, S., Sanchavanakit, N., In vitro biocompatibility of electrospun poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fiber mats (2007) Int J Biol Macromol, 40, pp. 217-223; 
Pritchard, C.D., Slotkin, J.R., Yu, D., Establishing a model spinal cord injury in the African green monkey for the preclinical evaluation of biodegradable polymer scaffolds seeded with human neural stem cells (2010) J Neurosci Meth, 188, pp. 258-269; 
Pires, L.R., Guarino, V., Barrias, C.C., Tunning poly(trymethylene carbonate-co-epsilon-caprolactone) fibers for nerve regeneration (2011) Histol Histopathol, 26, pp. 251-252; 
Tysseling, V.M., Sahni, V., Pashuck, E.T., Self-assembling peptide amphiphile promotes plasticity of serotonergic fibers following spinal cord injury (2010) J Neurosci Res, 88, pp. 3161-3170
},
	month = {2012///},
	pages = {2564 - 2582},
	abstract = {Spinal cord injury triggers a complex set of events that lead to tissue healing without the restoration of normal function due to the poor regenerative capacity of the spinal cord. Nevertheless, current knowledge about the intrinsic regenerative ability of central nervous system axons, when in a supportive environment, has made the prospect of treating spinal cord injury a reality. Among the range of strategies under investigation, cell-based therapies offer the most promising results, due to the multifactorial roles that these cells can fulfil. However, the best cell source is still a matter of debate, as are clinical issues that include the optimal cell dose as well as the timing and route of administration. In this context, the role of biomaterials is gaining importance. These can not only act as vehicles for the administered cells but also, in the case of chronic lesions, can be used to fill the permanent cyst, thus creating a more favourable and conducive environment for axonal regeneration in addition to serving as local delivery systems of therapeutic agents to improve the regenerative milieu. Some of the candidate molecules for the future are discussed in view of the knowledge derived from studying the mechanisms that facilitate the intrinsic regenerative capacity of central nervous system neurons. The future challenge for the multidisciplinary teams working in the field is to translate the knowledge acquired in basic research into effective combinatorial therapies to be applied in the clinic. {\textcopyright} 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.},
	keywords = {Biomaterials, Cell therapies, Clinical trials, Conditioning lesion, Gene therapy, Growth factors, Spinal cord injury, Stem cells},
	isbn = {15821838 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868098934\&partnerID=40\&md5=adb61a7d32054dc21aa4d733bc31427f},
	author = {P{\^e}go, A.P. and Kubinova, S. and Cizkova, D. and Vanicky, I. and Mar, F.M. and Sousa, M.M. and Sykova, E.}
}
@article {2195,
	title = {Staphylococcus aureus and Staphylococcus epidermidis adhesion to nanohydroxyapatite in the presence of model proteins},
	journal = {Biomedical Materials (Bristol)Biomed. Mater.},
	volume = {7},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArt. No.: 045010doi: 10.1088/1748-6041/7/4/045010Language of Original Document: EnglishCorrespondence Address: Ribeiro, M.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: ribeiro_marta88@hotmail.comChemicals/CAS: fibronectin, 86088-83-7; human serum albumin, 9048-49-1; hydroxyapatite, 1306-06-5, 51198-94-8References: Darley, E.S.R., MacGowan, A.P., Antibiotic treatment of Gram-positive bone and joint infections (2004) Journal of Antimicrobial Chemotherapy, 53 (6), pp. 928-935. , DOI 10.1093/jac/dkh191;Lidgren, L., Knutson, K., Stef{\'a}nsd{\'o}ttir, A., Infection of prosthetic joints (2003) Best Pract. Res. Clin. Rheumatol., 17, pp. 209-218; 
Wright, J.A., Nair, S.P., Interaction of staphylococci with bone (2010) Int. J. Med. Microbiol., 300, pp. 193-204; 
Harris, L.G., Richards, R.G., Staphylococci and implant surfaces: A review Injury Int (2006) J. Care Injured, 37, pp. 3-14; 
Cerca, N., Martins, S., Pier, G.B., Oliveira, R., Azeredo, J., The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm (2005) Research in Microbiology, 156 (5-6), pp. 650-655. , DOI 10.1016/j.resmic.2005.02.004, PII S0923250805000513; 
Vuong, C., Otto, M., Staphylococcus epidermidis infections (2002) Microbes and Infection, 4 (4), pp. 481-489. , DOI 10.1016/S1286-4579(02)01563-0, PII S1286457902015630; 
Montanaro, L., Campoccia, D., Arciola, C.R., Advancements in molecular epidemiology of implant infections and future perspectives (2007) Biomaterials, 28 (34), pp. 5155-5168. , DOI 10.1016/j.biomaterials.2007.08.003, PII S0142961207006175, Festschrift honouring Professor David F. Williams; 
Arciola, C.R., Campoccia, D., Gamberini, S., Donati, M.E., Pirini, V., Visai, L., Speziale, P., Montanaro, L., Antibiotic resistance in exopolysaccharide-forming Staphylococcus epidermidis clinical isolates from orthopaedic implant infections (2005) Biomaterials, 26 (33), pp. 6530-6535. , DOI 10.1016/j.biomaterials.2005.04.031, PII S0142961205003480; 
Raksujarit, A., Pengpat, K., Rujijanagul, G., Tunkasiri, T., Processing and properties of nanoporous hydroxyapatite ceramics (2010) Mater. Des., 31, pp. 1658-1660; 
Prabakaran, K., Rajeswari, S., Spectroscopic investigations on the synthesis of nano-hydroxyapatite from calcined eggshell by hydrothermal method using cationic surfactant as template (2009) Spectrochim. Acta A, 74, pp. 1127-1134; 
Kalita, S.J.A., Verma, Nanocrystalline hydroxyapatite bioceramic using microwave radiation: Synthesis and characterization (2010) Mater. Sci. Eng. C, 30, pp. 295-303; 
Teixeira, S., Rodriguez, M.A., Pena, P., De Aza, A.H., De Aza, S., Ferraz, M.P., Monteiro, F.J., Physical characterization of hydroxyapatite porous scaffolds for tissue engineering (2009) Mater. Sci. Eng. C, 29, pp. 1510-1514; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.M., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J. Appl. Biomater. Biomech., 2, pp. 74-80; 
Zhang, Z., Li, M., Chen, W., Zhu, S., Liu, N., Zhu, L., Immobilization of lead and cadmium from aqueous solution and contaminated sediment using nano-hydroxyapatite (2010) Environ. Pollut., 158, pp. 514-519; 
Zhang, L., Webster, T.J., Nanotechnology and nanomaterials: Promises for improved tissue regeneration (2009) Nano Today, 4, pp. 66-80; 
Ferraz, M.P., Mateus, A.Y., Sousa, J.C., Monteiro, F.J., Nanohydroxyapatite microspheres as delivery system for antibiotics: Release kinetics, antimicrobial activity, and interaction with osteoblasts (2007) Journal of Biomedical Materials Research - Part A, 81 (4), pp. 994-1004. , DOI 10.1002/jbm.a.31151; 
Oliveira, G.M., Ferraz, M.P., Gonz{\'a}lez, P., Serra, J., Leon, B., P{\`e}rez-Amor, M., Monteiro, F.J., PLD bioactive ceramic films: The influence of CaO-P 2O 5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells (2008) J. Mater. Sci. Mater. Med., 19, pp. 1775-1785; 
Campoccia, D., Montanaro, L., Arciola, C.R., The significance of infection related to orthopedic devices and issues of antibiotic resistance (2006) Biomaterials, 27 (11), pp. 2331-2339. , DOI 10.1016/j.biomaterials.2005.11.044, PII S0142961205010938; 
Tang, H., Wang, A., Liang, X., Cao, T., Salley, S.O., McAllister, J.P., Ng, K.Y., Effect of surface proteins on Staphylococcus epidermidis adhesion and colonization on silicone (2006) Colloids Surf. B, 51, pp. 16-24; 
Hu, X., Neoh, K.G., Shi, Z., Kang, E.T., Poh, C., Wang, W., An in vitro assessment of titanium functionalized with polysaccharides conjugated with vascular endothelial growth factor for enhanced osseointegration and inhibition of bacterial adhesion (2010) Biomaterials, 31, pp. 8854-8863; 
M{\"u}ller, R., Eidt, A., Hiller, K., Katzur, V., Subat, M., Schweikl, H., Imazato, S., Schmalz, G., Influences of protein films on antibacterial or bacteria-repellent surface coatings in a model system using silicon wafers (2009) Biomaterials, 30, pp. 4921-4929; 
An, Y.H., Friedman, R.J., Concise review of mechanisms of bacterial adhesion to biomaterial surfaces (1998) Journal of Biomedical Materials Research, 43 (3), pp. 338-348. , DOI 10.1002/(SICI)1097-4636(199823)43:3<338::AID-JBM16>3.0.CO;2-B; 
Katsikogianni, M., Missirlis, Y.F., Harris, L., Douglas, J., Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions (2004) European Cells and Materials, 8, pp. 37-57. , http://www.ecmjournal.org/journal/papers/vol008/pdf/v008a05.pdf; 
Liu, Y., Strauss, J., Camesano, T.A., Adhesion forces between Staphylococcus epidermidis and surfaces bearing self-assembled monolayers in the presence of model proteins (2008) Biomaterials, 29, pp. 4374-4382; 
Simpson, K.H., Bowden, M.G., Peacock, S.J., Arya, M., Hook, M., Anvari, B., Adherence of Staphylococcus aureus fibronectin binding protein a mutants: An investigation using optical tweezers (2004) Biomolecular Engineering, 21 (3-5), pp. 105-111. , DOI 10.1016/j.bioeng.2004.08.001, PII S1389034404000243; 
Simpson, K.H., Bowden, G., Hook, M., Anvari, B., Measurement of adhesive forces between individual Staphylococcus aureus MSCRAMMs and protein-coated surfaces by use of optical tweezers (2003) Journal of Bacteriology, 185 (6), pp. 2031-2035. , DOI 10.1128/JB.185.6.2031-2035.2003; 
Ribeiro, N., Sousa, S.R., Monteiro, F.J., Influence of crystallite size of nanophased hydroxyapatite on fibronectin and osteonectin adsorption and on MC3T3-E1 osteoblast adhesion and morphology (2010) J. Colloid Interface Sci., 351, pp. 398-406; 
Katsikogianni, M.G., Missirlis, Y.F., Interactions of bacteria with specific biomaterial surface chemistries under flow conditions (2010) Acta Biomater., 6, pp. 1107-1118; 
Buck, A.W., Fowler, V.G., Yongsunthon, R., Liu, J., DiBartola, A.C., Que, Y., Moreillon, P., Lower, S.K., Bonds between fibronectin and fibronectin-binding proteins on Staphylococcus aureus and Lactococcus lactis (2010) Langmuir, 26, pp. 10764-10770; 
Fonseca, A.P., Granja, P.L., Nogueira, J.A., Oliveira, D.R., Barbosa, M.A., Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives (2001) Journal of Materials Science: Materials in Medicine, 12 (6), pp. 543-548. , DOI 10.1023/A:1011227915575; 
Williams, R.J., Henderson, B., Nair, S.P., Staphylococcus aureus fibronectin binding proteins A and B possess a second fibronectin binding region that may have biological relevance to bone tissues (2002) Calcified Tissue International, 70 (5), pp. 416-421. , DOI 10.1007/s00223-001-2073-z; 
Williams, R.J., Henderson, B., Sharp, L.J., Nair, S.P., Identification of a fibronectin-binding protein from Staphylococcus epidermidis (2002) Infection and Immunity, 70 (12), pp. 6805-6810. , DOI 10.1128/IAI.70.12.6805-6810.2002; 
Tedjo, C., Neoh, K.G., Kang, E.T., Fang, N., Chan, V., Bacteria-surface interaction in the presence of proteins and surface attached poly(ethylene glycol) methacrylate chains (2007) Journal of Biomedical Materials Research - Part A, 82 (2), pp. 479-491. , DOI 10.1002/jbm.a.31172; 
Fowler, T., Wann, E.R., Joh, D., Johansson, S., Foster, T.J., H{\"o}{\"o}k, M., Cellular invasion by Staphylococcus aureus involves a fibronectin bridge between the bacterial fibronectin-binding MSCRAMMs and the host cell β1 integrins (2000) Eur. J. Cell Biol., 79, pp. 672-679; 
Peacock, S.J., Day, N.P.J., Thomas, M.G., Berendt, A.R., Foster, T.J., Clinical isolates of Staphylococcus aureus exhibit diversity in fnb genes and adhesion to human fibronectin (2000) Journal of Infection, 41 (1), pp. 23-31. , DOI 10.1053/jinf.2000.0657; 
Schwarz-Linek, U., Hook, M., Potts, J.R., Fibronectin-binding proteins of Gram-positive cocci (2006) Microbes and Infection, 8 (8), pp. 2291-2298. , DOI 10.1016/j.micinf.2006.03.011, PII S1286457906001663; 
Arciola, C.R., Bustanji, Y., Conti, M., Campoccia, D., Baldassarri, L., Samori, B., Montanaro, L., Staphylococcus epidermidis-fibronectin binding and its inhibition by heparin (2003) Biomaterials, 24 (18), pp. 3013-3019. , DOI 10.1016/S0142-9612(03)00133-9; 
Rohde, H., Frankenberger, S., Z{\"a}hringer, U., Mack, D., Structure, function and contribution of polysaccharide intercellular adhesin (PIA) to Staphylococcus epidermidis biofilm formation and pathogenesis of biomaterial-associated infections (2010) Eur. J. Cell Biol., 89, pp. 103-111; 
Christner, M., The giant extracellular matrix-binding protein of Staphylococcus epidermidis mediates biofilm accumulation and attachment to fibronectin (2010) Mol. Microbiol., 75, pp. 187-207; 
Del Prado, G., Ruiz, V., Naves, P., Rodr{\'\i}guez-Cerrato, V., Soriano, F., Del Carmen Ponte, M., Biofilm formation by Streptococcus pneumoniae strains and effects of human serum albumin, ibuprofen, N-acetyl-L-cysteine, amoxicillin, erythromycin, and levofloxac (2010) Diagn. Microbiol. Infect. Dis., 67, pp. 311-318; 
Charville, G.W., Hetrick, E.M., Geer, C.B., Schoenfisch, M.H., Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release (2008) Biomaterials, 29, pp. 4039-4044; 
Kinnari, T.J., Peltonen, L.I., Kuusela, P., Kivilahti, J., Kononen, M., Jero, J., Bacterial adherence to titanium surface coated with human serum albumin (2005) Otology and Neurotology, 26 (3), pp. 380-384. , DOI 10.1097/01.mao.0000169767.85549.87; 
Brokke, P., Dankert, J., Carballo, J., Feijen, J., Adherence of coagulase-negative staphylococci onto polyethylene catheters in vitro and in vivo: A study on the influence of various plasma proteins (1991) J. Biomater. Appl., 5, pp. 204-226; 
Saldarriaga, F.I.C., Van Der Mei, H.C., Lochhead, M.J., Grainger, D.W., Busscher, H.J., The inhibition of the adhesion of clinically isolated bacterial strains on multi-component cross-linked poly(ethylene glycol)-based polymer coatings (2007) Biomaterials, 28 (28), pp. 4105-4112. , DOI 10.1016/j.biomaterials.2007.05.023, PII S0142961207004395; 
Nayak, N., Satpathy, G., Nag, H.L., Venkatesh, P., Ramakrishnan, S., Nag, T.C., Prasad, S., Slime production is essential for the adherence of Staphylococcus epidermidis in implant-related infections (2011) J. Hosp. Infect., 77, pp. 153-156
},
	month = {2012///},
	abstract = {Bacterial infections can have adverse effects on the efficacy, lifetime, and safety of an implanted device. The aim of this study was to investigate the initial adhesion of several strains, namely S. aureus and S. epidermidis, on two distinct types of nanohydroxyapatite (nanoHA), sintered at 725{\textdegree}C and 1000{\textdegree}C. A comparison was also made with nanohydroxyapatite having adsorbed fetal bovine serum (FBS), human fibronectin (FN) and human serum albumin (HSA). Adhered bacterial cells were examined by scanning electron microscopy and quantified as colony forming units after being released by sonication. The wettability of the sample surface with and without adsorbed protein was assessed by contact-angle measurements. NanoHA sintered at 1000{\textdegree}C showed lower bacterial adhesion than this heat-treated at 725{\textdegree}C. Adsorption of FBS onto the nanoHA surface caused a decrease in the adhesion of all strains on both materials. The bacterial adhesion patterns in the presence of FN were different for both nanoHA substrates; the adherence of the bacterial strains, except for the clinical strain of S.epidermidis, was significantly higher on nanoHA 1000 in comparison to nanoHA 1000 without protein and the bacterial adhesion on the FN-coated nanoHA 725 was lower in comparison to the bare nanoHA 725. The effect of HSA on bacterial adhesion was concentration and bacterial strain dependent. {\textcopyright} 2012 IOP Publishing Ltd.},
	keywords = {Adhesion, Adsorption, Adverse effect, article, Bacteria, Bacterial adhesion, bacterial cell, Bacterial cells, Bacterial infections, bacterial strain, Bacterial strains, bacterium adherence, Clinical strains, colony forming unit, Colony forming units, Contact angle, Contact-angle measurements, controlled study, Enzyme inhibition, Epidermidis, Fetal bovine serum, Fibronectin, heating, Human fibronectin, human serum albumin, Human serum albumins, hydroxyapatite, Implanted device, materials testing, Model proteins, Nano-HA, Nano-hydroxyapatite, Nanohydroxyapatite, nanoparticle, nonhuman, Proteins, S. aureus, Sample surface, Scanning electron microscopy, serum, Sintering, Staphylococcus aureus, Staphylococcus epidermidis, surface property, ultrasound, unclassified drug, wettability},
	isbn = {17486041 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864391456\&partnerID=40\&md5=9170b87ade5223df5146d21981d073ef},
	author = {Ribeiro, M. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {2212,
	title = {Synthesis of an O-alkynyl-chitosan and its chemoselective conjugation with a PEG-like amino-azide through click chemistry},
	journal = {Carbohydrate PolymersCarbohydr Polym},
	volume = {87},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: CAPODdoi: 10.1016/j.carbpol.2011.07.043Language of Original Document: EnglishCorrespondence Address: Gomes, P.; CIQ-UP, Departamento de Qu{\'\i}mica e Bioqu{\'\i}mica, Universidade Do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal; email: pgomes@fc.up.ptReferences: Amaral, I.F., Granja, P., Barbosa, M.A., Chemical modification of chitosan by phosphorylation: An XPS, FT-IR and SEM study (2005) Journal of Biomaterials Science, Polymer Edition, 16, pp. 1575-1593;Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cell osteogenic differentiation and fibronectin adsorption on chitosan membranes: The effect of the degree of acetylation (2005) Journal of Biomedical Materials Research, 75 A, pp. 387-397; 
Anitha, A., Deepa, N., Chennazhi, K.P., Nair, S.V., Tamura, H., Jayakumar, R., Development of muchoadhesive, thiolated chitosan nanoparticles for biomedical applications (2011) Carbohydrate Polymers, 83, pp. 66-73; 
Batista, M.K.S., Gallem{\'\i}, M., Adeva, A., Gomes, C.A.R., Gomes, P., Facile regioselective synthesis of a novel chitosan-pexiganan conjugate with potential interest for the treatment of infected skin lesions (2009) Synthetic Communications, 39, pp. 1228-1240; 
Batista, M.K.S., Pinto, L.F., Gomes, C.A.R., Gomes, P., Novel highly-soluble peptide-chitosan polymers: Synthesis and spectral characterization (2006) Carbohydrate Polymers, 64, pp. 299-305; 
Beamson, G., Briggs, D., (1992) High Resolution XPS of Organic Polymers: The Scientia ESCA300 Database, , John Wiley \& Sons New York (USA); 
Bennett, A.E., Rienstra, C.M., Auger, M., Lakshmi, K.V., Griffin, R.G., Heteronuclear decoupling in rotating solids (1995) The Journal of Chemical Physics, 103, pp. 6951-6958; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57, pp. 35-52; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Peppas, N.A., Gurny, R., Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57, pp. 19-34; 
Bhatnagar, A., Sillanp{\"a}{\"a}, M., Applications of chitin- and chitosan-derivatives for the detoxification of water and wastewater: A short review (2009) Advances in Colloid and Interface Science, 152, pp. 26-38; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arg{\"u}elles-Monal, W., Desbrires, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Cabrera, J.C., Van Cutsem, P., Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan (2005) Biochemical Engineering Journal, 25, pp. 165-172; 
Chinchilla, R., N{\'a}jera, C., The Sonogashira reaction: A booming methodology in synthetic organic chemistry (2007) Chemical Reviews, 107, pp. 874-922; 
Chirachanchai, S., Lertworasirikul, A., Tachaboonyakiat, W., Carbaryl insecticide conjugation onto chitosan via iodochitosan and chitosan carbonyl imidazolide precursors (2001) Carbohydrate Polymers, 46, pp. 19-27; 
Crompton, K.E., Goud, J.D., Bellamkonda, R.V., Gengenbach, T.R., Finkelstein, D.I., Horne, M.K., Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering (2007) Biomaterials, 28, pp. 441-449; 
Dai, T., Tegos, G.P., Burkatovskaya, M., Castano, A.P., Hamblin, M.R., Chitosan acetate bandage as a topical antimicrobial dressing for infected burns (2009) Antimicrobial Agents and Chemotherapy, 53, pp. 393-400; 
Elkholy, S.S., Khalil, K.D., Elsabee, M.Z., Grafting of acryloyl cyanoacetohydrazide onto chitosan (2011) Journal of Polymer Research, 18, pp. 459-467; 
Gomes, P., Gomes, C.A.R., Batista, M.K.S., Pinto, L.F., Silva, P.A.P., Synthesis, structural characterization and properties of water-soluble N-(γ-propanoyl-amino acid)-chitosans (2008) Carbohydrate Polymers, 71, pp. 54-65; 
Holappa, J., Hj{\'a}lmarsd{\'o}ttir, M., M{\'a}sson, M., R{\'u}narsson, {\"O}., Asplund, T., Soininen, P., Antimicrobial activity of chitosan N-betainates (2006) Carbohydrate Polymers, 65, pp. 114-118; 
Holappa, J., Nevalainen, T., Soininen, P., M{\'a}sson, M., J{\"a}rvinen, T., Synthesis of novel quaternary chitosan derivatives via N-chloroacyl-6-O-triphenylmethylchitosans (2006) Biomacromolecules, 7, pp. 407-410; 
Iqbal, J., Sarti, F., Perera, G., Bernkop-Schn{\"u}rch, A., Development and in vivo evaluation of an oral drug delivery system for paclitaxel (2011) Biomaterials, 32, pp. 170-175; 
Kolb, H.C., Finn, M.G., Sharpless, K.B., Click Chemistry: Diverse chemical function from a few good reactions (2001) Angewandte Chemie International Edition, 40, pp. 2004-2021; 
Krishnakumar, V., John Xavier, R., FT Raman and FT-IR spectral studies of 3-mercapto-1,2,4-triazole (2004) Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 60, pp. 709-714; 
Kulbokaite, R., Ciuta, G., Netopilik, M., Makuska, R., N-PEGylation of chitosan via click reactions (2009) Reactive and Functional Polymers., 69, pp. 771-778; 
Kurita, K., Ikeda, H., Yoshida, Y., Shimojoh, M., Harata, M., Chemoselective protection of the amino groups of chitosan by controlled phthaloylation: Facile preparation of a precursor useful for chemical modifications (2002) Biomacromolecules, 3, pp. 1-4; 
Kurita, K., Yoshida, Y., Umemura, T., Finely selective protections and deprotections of multifunctional chitin and chitosan to synthesize key intermediates for regioselective chemical modifications (2010) Carbohydrate Polymers, 81, pp. 434-440; 
Lahann, J., (2009) Click Chemistry for Biotechnology and Materials Science, , John Wiley \& Sons Chichester (UK); 
Lallana, E., Fern{\'a}ndez-Meg{\'\i}a, E., Riguera, R., Surpassing the use of copper in the click functionalization of polymeric nanostructures: A strain promoted approach (2009) Journal of the American Chemical Society., 131, pp. 5748-5750; 
Li, X., Yuan, W., Gu, S., Re, J., Synthesis and self-assembly of tunable thermosensitive chitosan amphiphilic copolymers by click chemistry (2010) Materials Letters, 64, pp. 2663-2666; 
Maku{\v s}ka, R., Gorochovceva, N., Regioselective grafting of poly(ethylene glycol) onto chitosan through C-6 position of glucosamine units (2006) Carbohydrate Polymers, 64, pp. 319-327; 
Meldal, M., Tom{\o}e, C.W., Cu-catalyzed azide-alkyne cycloaddition (2008) Chemical Reviews, 108, pp. 2952-3015; 
Moln{\'a}r, E., Barbu, E., Lien, C.-F., G{\'o}recki, D.C., Tsibouklis, J., Toward drug delivery into the brain: Synthesis, characterization, and preliminary in vitro assessment of alkylglyceryl-functionalized chitosan nanoparticles (2010) Biomacromolecules, 11, pp. 2880-2889; 
Montalbetti, C.A.G.N., Falque, V., Amide bond formation and peptide coupling (2005) Tetrahedron, 61, pp. 10827-10852; 
Muzzarelli, R.A.A., Carboxymethylated chitins and chitosans (1988) Carbohydrate Polymers, 8, pp. 1-21; 
Muzzarelli, R.A.A., Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone (2009) Carbohydrate Polymers, 76, pp. 167-182; 
Muzarelli, R.A.A., Chitosan composites with inorganics, morphogenetic proteins and stem cells, for bone regeneration (2011) Carbohydrate Polymers, 83, pp. 1433-1445; 
Oh, J.K., Lee, D.I., Park, J.M., Biopolymer-based microgels/nanogels for drug delivery applications (2009) Progress in Polymer Science, 34, pp. 1261-1282; 
{\"O}nal, S., Telefoncu, A., Comparison of chitin and amberlite IRA-938 for a-galactosidase immobilization (2003) Artifical Cells, Blood Substitutes, and Biotechnology, 31, pp. 19-33; 
{\"O}zba-Turan, S., Akbua, J., Sezer, A.D., Topical application of antisense oligonucleotide-loaded chitosan nanoparticles to rats (2010) Oligonucleotides, 20, pp. 147-153; 
Park, S.O., Kwon, O.S., Choi, C.W., Kim, C.-J., Glucosamine-mediated detoxification of p-benzoquinone and its removal by chitosan (2000) Biotechnology Letters, 22, pp. 21-24; 
Prabuh, K., Sundaramoorthi, V., Selvaraju, C.K., Tamilselvi, K.K.N., Topical immunization and the use of chitosan as carrier (2011) International Journal of Research in Ayurveda and Pharmacy, 2, pp. 90-94; 
Shen, X., Ji, Y., Yang, Q., Zheng, X., Preparation, characterization, and rheological properties of dibutyrylchitin (2007) Journal of Macromolecular Science B, 49, pp. 250-258; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies Tables and Charts, , 3rd edition John Wiley \& Sons, Ltd New York (USA); 
R{\'u}narsson, {\"O}., Holappa, J., J{\'o}nsd{\'o}ttir, S., Steinsson, H., M{\'a}sson, M., N-selective {\textquoteright}one-pot{\textquoteright} synthesis of highly substituted trimethyl chitosan (TMC) (2008) Carbohydrate Polymers, 74, pp. 740-744; 
R{\'u}narsson, {\"O}., Holappa, J., Malainer, C., Steinsson, H., Hj{\'a}lmarsd{\'o}ttir, M., Nevalainen, T., Antibacterial activity of N-quaternary chitosan derivatives: Synthesis, characterization and structure activity relationship (SAR) investigations (2010) European Polymer Journal, 46, pp. 1251-1267; 
Wan, A., Gao, Q., Li, H., Effects of molecular weight and degree of acetylation on the release of nitric oxide from chitosan-nitric oxide adducts (2010) Journal of Applied Polymer Science, 117, pp. 2183-2188; 
Yuan, W., Li, X., Gu, S., Cao, A., Ren, J., Amphiphilic chitosan graft copolymer via combination of ROP, ATRP and click chemistry: Synthesis, self-assembly, thermosensitivity, fluorescence, and controlled drug release (2011) Polymer, 52, pp. 658-666; 
Yuan, W., Zhao, Z., Gu, S., Ren, J., Synthesis, characterization, and properties of amphiphilic chitosan copolymers with mixed side chains by click chemistry (2010) Journal of Polymer Science A, 48, pp. 3476-3486; 
Yuan, W., Zhao, Z., Gu, S., Ren, T., Ren, J., Synthesis and self-assembly of pH-responsive chitosan graft copolymer by the combination of atom transfer radical polymerization and click chemistry (2011) Materials Letters, 65, pp. 793-796; 
Zhang, J., Li, C., Xue, Z.-Y., Cheng, H.-W., Huang, F.-W., Zhuo, R.-X., Fabrication of lactobionic-loaded chitosan microcapsules as potential drug carriers targeting the liver (2011) Acta Biomaterialia, 7, pp. 1665-1673
},
	month = {2012///},
	pages = {240 - 249},
	abstract = {N-Phthaloyl-chitosan O-prop-2-ynyl carbamate was prepared as a biopolymer amenable to undergo chemoselective conjugation by azide-alkyne coupling, while allowing upturn of chitosan{\textquoteright}s amines after dephthaloylation. N-phthaloylchitosan was prepared according to previously described methods and, due to its low solubility in current organic media, subsequent modifications were run in heterogeneous conditions. Activation of hydroxyls with carbonyl-1,1'- diimidazole and coupling to propargylamine yielded N-phthaloyl-chitosan O-prop-2-ynyl carbamate, then coupled to a model PEG-like azide by azide-alkyne coupling, giving the expected triazolyl conjugate. N-Dephthaloylation allowed recovery of the free amines, responsible for chitosan{\textquoteright}s bioadhesion and tissue-regeneration properties. The structures of all polymers were confirmed by Fourier-transformed infra-red (FT-IR) and X-ray photoelectron (XPS) spectroscopies, as well as by solid-state nuclear magnetic resonance (SSNMR). All chitosan derivatives were poorly soluble in both aqueous and organic media, which makes them suitable for topical applications or for removal of toxic substances from either the gastric intestinal tract or environmental sources. {\textcopyright} 2011 Elsevier Ltd. All Rights Reserved.},
	keywords = {1 ,3-Dipolarcycloaddition, Acetylene, Amines, Azide-alkyne coupling, Bio-adhesion, Bioadhesion, Biomaterials, chitosan, click chemistry, Cycloaddition, FTIR, Huisgen{\textquoteright}s 1,3-dipolar cycloaddition, Nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy, Photoelectron spectroscopy, Resonance, Size exclusion chromatography, Size-exclusion chromatography, Solid state NMR, Solid-state NMR, Tissue, X ray photoelectron spectroscopy, XPS},
	isbn = {01448617 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80054771159\&partnerID=40\&md5=3760f41563573759f0ce676fe813d6c6},
	author = {Oliveira, J.R. and Martins, M.C.L. and Mafra, L. and Gomes, P.}
}
@article {2210,
	title = {Tumor cell-educated periprostatic adipose tissue acquires an aggressive cancer-promoting secretory profile},
	journal = {Cellular Physiology and BiochemistryCell. Physiol. Biochem.},
	volume = {29},
	year = {2012},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: CEPBEdoi: 10.1159/000337604PubMed ID: 22415092Language of Original Document: EnglishCorrespondence Address: Ribeiro, R.J.T.; Portuguese Institute of Oncology, Porto Centre Molecular Oncology Group -CI, Edif{\'\i}cio Laborat{\'o}rios -Piso 4, Rua Dr. Ant{\'o}nio Bernardino Almeida, 4200-072 Porto, Portugal; email: oriebir.r@gmail.comChemicals/CAS: adiponectin, 283182-39-8; gelatinase B, 146480-36-6; osteopontin, 106441-73-0; Adiponectin; Culture Media, Conditioned; Cytokines; DNA, Mitochondrial; Interleukin-6; Matrix Metalloproteinase 2, 3.4.24.24; Matrix Metalloproteinase 9, 3.4.24.35; Osteopontin, 106441-73-0; Tumor Necrosis Factor-alphaReferences: Hsing, A.W., Sakoda, L.C., Chua Jr., S., Obesity, metabolic syndrome, and prostate cancer (2007) Am J Clin Nutr, 86, pp. s843-857;Cao, Y., Ma, J., Body mass index, prostate cancer-specific mortality, and biochemical recurrence: A systematic review and meta-analysis (2011) Cancer Prev Res (Phila), 4, pp. 486-501; 
Baillargeon, J., Rose, D.P., Obesity, adipokines, and prostate cancer (review) (2006) Int J Oncol, 28, pp. 737-745; 
Mistry, T., Digby, J.E., Desai, K.M., Randeva, H.S., Obesity and Prostate Cancer: A Role for Adipokines (2007) European Urology, 52 (1), pp. 46-53. , DOI 10.1016/j.eururo.2007.03.054, PII S0302283807004307; 
Hirano, T., Morii, H., Nakazawa, K., Murakami, D., Yamashita, A., Asahi, J., Orimo, H., Tokura, Y., Enhancement of adipogenesis induction by conditioned media obtained from cancer cells (2008) Cancer Lett, 268, pp. 286-294; 
Andarawewa, K.L., Motrescu, E.R., Chenard, M.-P., Gansmuller, A., Stoll, I., Tomasetto, C., Rio, M.-C., Stromelysin-3 is a potent negative regulator of adipogenesis participating to cancer cell-adipocyte interaction/crosstalk at the tumor invasive front (2005) Cancer Research, 65 (23), pp. 10862-10871. , DOI 10.1158/0008-5472.CAN-05-1231; 
Jotzu, C., Alt, E., Welte, G., Li, J., Hennessy, B.T., Devarajan, E., Krishnappa, S., Song, Y.H., Adipose tissue derived stem cells differentiate into carcinoma-associated fibroblast-like cells under the influence of tumor derived factors (2011) Cell Oncol (Dordr), 34, pp. 55-67; 
Dolinkova, M., Dostalova, I., Lacinova, Z., Michalsky, D., Haluzikova, D., Mraz, M., The endocrine profile of subcutaneous and visceral adipose tissue of obese patients (2008) Mol Cell Endocrinol, 291, pp. 63-70; 
Van Roermund, J.G., Hinnen, K.A., Tolman, C.J., Bol, G.H., Witjes, J.A., Bosch, J.L., Kiemeney, L.A., Van Vulpen, M., Periprostatic fat correlates with tumour aggressiveness in prostate cancer patients (2011) BJU Int, 107, pp. 1775-1779; 
Sumitomo, M., Asakuma, J., Yoshii, H., Sato, A., Horiguchi, A., Ito, K., Nagakura, K., Asano, T., Anterior perirectal fat tissue thickness is a strong predictor of recurrence after high-intensity focused ultrasound for prostate cancer (2010) Int J Urol, 17, pp. 776-782; 
Finley, D.S., Calvert, V.S., Inokuchi, J., Lau, A., Narula, N., Petricoin, E.F., Zaldivar, F., Ornstein, D.K., Periprostatic adipose tissue as a modulator of prostate cancer aggressiveness (2009) J Urol, 182, pp. 1621-1627; 
Thalmann, S., Juge-Aubry, C.E., Meier, C.A., Explant cultures of white adipose tissue (2008) Methods Mol Biol, 456, pp. 195-199; 
Song, G.J., Lewis, V., Mitochondrial DNA integrity and copy number in sperm from infertile men (2008) Fertil Steril, 90, pp. 2238-2244; 
Park, J., Euhus, D.M., Scherer, P.E., Paracrine and Endocrine Effects of Adipose Tissue on Cancer Development and Progression (2011) Endocr Rev, 32, pp. 550-570; 
George, D.J., Halabi, S., Shepard, T.F., Sanford, B., Vogelzang, N.J., Small, E.J., Kantoff, P.W., The prognostic significance of plasma interleukin-6 levels in patients with metastatic hormone-refractory prostate cancer: Results from cancer and leukemia group B 9480 (2005) Clinical Cancer Research, 11 (5), pp. 1815-1820. , DOI 10.1158/1078-0432.CCR-04-1560; 
Michalaki, V., Syrigos, K., Charles, P., Waxman, J., Serum levels of IL-6 and TNF-α correlate with clinicopathological features and patient survival in patients with prostate cancer (2004) British Journal of Cancer, 90 (12), pp. 2312-2316; 
Dirat, B., Bochet, L., Dabek, M., Daviaud, D., Dauvillier, S., Majed, B., Wang, Y.Y., Muller, C., Cancerassociated adipocytes exhibit an activated phenotype and contribute to breast cancer invasion (2011) Cancer Res, 71, pp. 2455-2465; 
Culig, Z., New insights into the role of interleukin-6 in human prostate cancer (2009) J Urol, 182, pp. 1255-1256; 
Chun, J.Y., Nadiminty, N., Dutt, S., Lou, W., Yang, J.C., Kung, H.J., Evans, C.P., Gao, A.C., Interleukin-6 regulates androgen synthesis in prostate cancer cells (2009) Clin Cancer Res, 15, pp. 4815-4822; 
Weber, G.F., Lett, G.S., Haubein, N.C., Osteopontin is a marker for cancer aggressiveness and patient survival (2010) Br J Cancer, 103, pp. 861-869; 
Thalmann, G.N., Sikes, R.A., Devoll, R.E., Kiefer, J.A., Markwalder, R., Klima, I., Farach-Carson, C.M., Chung, L.W.K., Osteopontin: Possible role in prostate cancer progression (1999) Clinical Cancer Research, 5 (8), pp. 2271-2277; 
McAllister, S.S., Gifford, A.M., Greiner, A.L., Kelleher, S.P., Saelzler, M.P., Ince, T.A., Reinhardt, F., Weinberg, R.A., Systemic Endocrine Instigation of Indolent Tumor Growth Requires Osteopontin (2008) Cell, 133 (6), pp. 994-1005. , DOI 10.1016/j.cell.2008.04.045, PII S0092867408006211; 
Balkwill, F., TNF-alpha in promotion and progression of cancer (2006) Cancer Metastasis Rev, 25, pp. 409-416; 
Yan, B., Wang, H., Rabbani, Z.N., Zhao, Y., Li, W., Yuan, Y., Li, F., Li, C.-Y., Tumor necrosis factor-α is a potent endogenous mutagen that promotes cellular transformation (2006) Cancer Research, 66 (24), pp. 11565-11570. , DOI 10.1158/0008-5472.CAN-06-2540; 
Bates, R.C., Mercurio, A.M., Tumor necrosis factor-α stimulates the epithelial-tomesenchymal transition of human colonic organoids (2003) Molecular Biology of the Cell, 14 (5), pp. 1790-1800. , DOI 10.1091/mbc.E02-09-0583; 
Yamauchi, T., Kamon, J., Waki, H., Terauchi, Y., Kubota, N., Hara, K., Mori, Y., Kadowaki, T., The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity (2001) Nature Medicine, 7 (8), pp. 941-946. , DOI 10.1038/90984; 
Li, H., Stampfer, M.J., Mucci, L., Rifai, N., Qiu, W., Kurth, T., Ma, J., A 25-year prospective study of plasma adiponectin and leptin concentrations and prostate cancer risk and survival (2010) Clin Chem, 56, pp. 34-43; 
Ribeiro, R., Lopes, C., Medeiros, R., The link between obesity and prostate cancer: The leptin pathway and therapeutic perspectives (2006) Prostate Cancer and Prostatic Diseases, 9 (1), pp. 19-24. , DOI 10.1038/sj.pcan.4500844, PII 4500844; 
Vaday, G.G., Peehl, D.M., Kadam, P.A., Lawrence, D.M., Expression of CCL5 (RANTES) and CCR5 in prostate cancer (2006) Prostate, 66 (2), pp. 124-134. , DOI 10.1002/pros.20306; 
Kessenbrock, K., Plaks, V., Werb, Z., Matrix metalloproteinases: Regulators of the tumor microenvironment (2010) Cell, 141, pp. 52-67; 
Bouloumie, A., Sengenes, C., Portolan, G., Galitzky, J., Lafontan, M., Adipocyte Produces Matrix Metalloproteinases 2 and 9 Involvement in Adipose Differentiation (2001) Diabetes, 50 (9), pp. 2080-2086; 
Bergers, G., Brekken, R., McMahon, G., Vu, T.H., Itoh, T., Tamaki, K., Tanzawa, K., Hanahan, D., Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis (2000) Nat Cell Biol, 2, pp. 737-744; 
Lin, C.Y., Tsai, P.H., Kandaswami, C.C., Lee, P.P., Huang, C.J., Hwang, J.J., Lee, M.T., Matrix metalloproteinase-9 cooperates with transcription factor Snail to induce epithelial-mesenchymal transition (2011) Cancer Sci, 102, pp. 815-827; 
Chakrabarti, S., Patel, K.D., Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology (2005) Experimental Lung Research, 31 (6), pp. 599-621. , DOI 10.1080/019021490944232; 
Sehgal, G., Hua, J., Bernhard, E.J., Sehgal, I., Thompson, T.C., Muschel, R.J., Requirement for matrix metalloproteinase-9 (Gelatinase B) expression in metastasis by murine prostate carcinoma (1998) American Journal of Pathology, 152 (2), pp. 591-596; 
Johnson, T.R., Koul, S., Kumar, B., Khandrika, L., Venezia, S., Maroni, P.D., Meacham, R.B., Koul, H.K., Loss of PDEF, a prostatederived Ets factor is associated with aggressive phenotype of prostate cancer: Regulation of MMP 9 by PDEF (2010) Mol Cancer, 9, p. 148; 
Trudel, D., Fradet, Y., Meyer, F., Tetu, B., Matrix metalloproteinase 9 is associated with Gleason score in prostate cancer but not with prognosis (2010) Hum Pathol, 41, pp. 1694-1701; 
Zhang, L., Shi, J., Feng, J., Klocker, H., Lee, C., Zhang, J., Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer (2004) Prostate Cancer and Prostatic Diseases, 7 (4), pp. 327-332. , DOI 10.1038/sj.pcan.4500750, PII 4500750; 
Zhong, W.D., Han, Z.D., He, H.C., Bi, X.C., Dai, Q.S., Zhu, G., Ye, Y.K., Chen, Z.N., CD147, MMP-1, MMP-2 and MMP-9 protein expression as significant prognostic factors in human prostate cancer (2008) Oncology, 75, pp. 230-236; 
Sauer, C.G., Kappeler, A., Spath, M., Kaden, J.J., Michel, M.S., Mayer, D., Bleyl, U., Grobholz, R., Expression and activity of matrix metalloproteinases-2 and -9 in serum, core needle biopsies and tissue specimens of prostate cancer patients (2004) Virchows Archiv, 444 (6), pp. 518-526; 
Opdenakker, G., Masure, S., Grillet, B., Van Damme, J., Cytokine-mediated regulation of human leukocyte gelatinases and role in arthritis (1991) Lymphokine Cytokine Res, 10, pp. 317-324; 
Unal, R., Yao-Borengasser, A., Varma, V., Rasouli, N., Labbate, C., Kern, P.A., Ranganathan, G., Matrix metalloproteinase-9 is increased in obese subjects and decreases in response to pioglitazone (2010) J Clin Endocrinol Metab, 95, pp. 2993-3001; 
Catalan, V., Gomez-Ambrosi, J., Rodriguez, A., Ramirez, B., Silva, C., Rotellar, F., Gil, M.J., Fr{\"u}hbeck, G., Increased adipose tissue expression of lipocalin-2 in obesity is related to inflammation and matrix metalloproteinase-2 and metalloproteinase-9 activities in humans (2009) J Mol Med (Berl), 87, pp. 803-813; 
Villarroya, J., Giralt, M., Villarroya, F., Mitochondrial DNA: An up-and-coming actor in white adipose tissue pathophysiology (2009) Obesity (Silver Spring), 17, pp. 1814-1820; 
Robin, E.D., Wong, R., Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells (1988) J Cell Physiol, 136, pp. 507-513; 
Kaaman, M., Sparks, L.M., Van Harmelen, V., Smith, S.R., Sjolin, E., Dahlman, I., Arner, P., Strong association between mitochondrial DNA copy number and lipogenesis in human white adipose tissue (2007) Diabetologia, 50 (12), pp. 2526-2533. , DOI 10.1007/s00125-007-0818-6; 
Yamauchi, T., Kamon, J., Waki, H., Murakami, K., Motojima, K., Komeda, K., Ide, T., Kadowaki, T., The mechanisms by which both heterozygous peroxisome proliferator- activated receptor gamma (PPARgamma) deficiency and PPARgamma agonist improve insulin resistance (2001) J Biol Chem, 276, pp. 41245-41254; 
Wilson-Fritch, L., Burkart, A., Bell, G., Mendelson, K., Leszyk, J., Nicoloro, S., Czech, M., Corvera, S., Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone (2003) Molecular and Cellular Biology, 23 (3), pp. 1085-1094. , DOI 10.1128/MCB.23.3.1085-1094.2003; 
Ducluzeau, P.H., Priou, M., Weitheimer, M., Flamment, M., Duluc, L., Iacobazi, F., Soleti, R., Malthi{\`e}ry, Y., Dynamic regulation of mitochondrial network and oxidative functions during 3T3-L1 fat cell differentiation (2011) J Physiol Biochem, 67, pp. 285-296; 
Bogacka, I., Ukropcova, B., McNeil, M., Gimble, J.M., Smith, S.R., Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro (2005) Journal of Clinical Endocrinology and Metabolism, 90 (12), pp. 6650-6656. , http://jcem.endojournals.org/cgi/reprint/90/12/6650, DOI 10.1210/jc.2005-1024; 
Toda, S., Uchihashi, K., Aoki, S., Sonoda, E., Yamasaki, F., Piao, M., Ootani, A., Sugihara, H., Adipose tissueorganotypic culture system as a promising model for studying adipose tissue biology and regeneration (2009) Organogenesis, 5, pp. 50-56; 
Tuxhorn, J.A., Ayala, G.E., Rowley, D.R., Reactive stroma in prostate cancer progression (2001) Journal of Urology, 166 (6), pp. 2472-2483
},
	month = {2012///},
	pages = {233 - 240},
	abstract = {Background/Aims: The microenvironment produces important factors that are crucial to prostate cancer (PCa) progression. However, the extent to which the cancer cells stimulate periprostatic adipose tissue (PPAT) to produce these proteins is largely unknown. Our purpose was to determine whether PCa cell-derived factors influence PPAT metabolic activity. Methods: Primary cultures of human PPAT samples from PCa patients (adipose tissue organotypic explants and primary stromal vascular fraction, SVF) were stimulated with conditioned medium (CM) collected from prostate carcinoma (PC3) cells. Cultures without CM were used as control. We used multiplex analysis and ELISA for protein quantification, qPCR to determine mitochondrial DNA (mtDNA) copy number and zymography for matrix metalloproteinase activity, in order to evaluate the response of adipose tissue explants and SVFs to PC3 CM. Results: Stimulation of PPAT explants with PCa PC3 CM induced adipokines associated with cancer progression (osteopontin, tumoral necrosis factor alpha and interleukin-6) and reduced the expression of the protective adipokine adiponectin. Notably, osteopontin protein expression was 13-fold upregulated. Matrix metalloproteinase 9 activity and mitochondrial DNA copy number were higher after stimulation with cancer CM. Stromovascular cells from PPAT in culture were not influenced by tumor-derived factors. Conclusion: The modulation of adipokine expression by tumor CM indicates the pervasive extent to which tumor cells command PPAT to produce factors favorable to their aggressiveness. {\textcopyright} 2012 S. Karger AG, Basel.},
	keywords = {Adipokines, adiponectin, adipose tissue, adult, article, cancer cell, cancer growth, Cells, Cultured, clinical article, controlled study, Culture Media, Conditioned, culture medium, Cytokines, DNA, Mitochondrial, enzyme activity, enzyme linked immunosorbent assay, explant, gelatinase B, human, human cell, human tissue, Humans, interleukin 6, Interleukin-6, male, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Middle Aged, mitochondrial DNA, Osteopontin, Periprostatic adipose tissue, priority journal, Prostate cancer, Prostatic Neoplasms, protein expression, protein induction, protein secretion, quantitative analysis, stroma cell, tissue metabolism, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha},
	isbn = {10158987 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859829669\&partnerID=40\&md5=a76be7e5c0037e77140c1fc9aca48a5a},
	author = {Ribeiro, R.J.T. and Monteiro, C.P.D. and Cunha, V.F.P.M. and Azevedo, A.S.M. and Oliveira, M.J. and Monteiro, R. and Fraga, A.M. and Pr{\'\i}ncipe, P. and Lobato, C. and Lobo, F. and Morais, A. and Silva, V. and Sanches-Magalh{\~a}es, J. and Oliveira, J. and Guimar{\~a}es, J.T. and Lopes, C.M.S. and Medeiros, R.M.}
}
@article {2241,
	title = {The Arabidopsis D-Type Cyclin CYCD2;1 and the Inhibitor ICK2/KRP2 Modulate Auxin-Induced Lateral Root Formation},
	journal = {Plant CellPlant Cell},
	volume = {23},
	year = {2011},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: ScopusCODEN: PLCEEdoi: 10.1105/tpc.110.080002PubMed ID: 21357490Language of Original Document: EnglishCorrespondence Address: Murray, J. A. H.; Cardiff School of Biosciences, Cardiff University, CF10 3AX Cardiff, United Kingdom; email: murrayja1@cardiff.ac.ukChemicals/CAS: proteasome, 140879-24-9; ATP dependent 26S protease, 3.4.99.-; Arabidopsis Proteins; Cell Cycle Proteins; CycD2 protein, Arabidopsis; Cyclins; ICK2 protein, Arabidopsis; Indoleacetic Acids; Proteasome Endopeptidase Complex, 3.4.25.1References: Achard, P., Gusti, A., Cheminant, S., Alioua, M., Dhondt, S., Coppens, F., Beemster, G.T., Genschik, P., Gibberellin signaling controls cell proliferation rate in Arabidopsis (2009) Curr. Biol., 19, pp. 1188-1193;Alonso, J.M., Genome-wide insertional mutagenesis of Arabidopsis thaliana (2003) Science, 301, pp. 653-657; 
Baluska, F., Mancuso, S., Volkmann, D., Barlow, P.W., Root apex transition zone: A signalling-response nexus in the root (2010) Trends Plant Sci., 15, pp. 402-408; 
Bechtold, N., Pelletier, G., In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration (1998) Methods Mol. Biol., 82, pp. 259-266; 
Bird, D.A., Buruiana, M.M., Zhou, Y., Fowke, L.C., Wang, H., Arabidopsis cyclin-dependent kinase inhibitors are nuclearlocalized and show different localization patterns within the nucleoplasm (2007) Plant Cell Rep., 26, pp. 861-872; 
Boerjan, W., Cervera, M.T., Delarue, M., Beeckman, T., Dewitte, W., Bellini, C., Caboche, M., Inz{\'e}, D., Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction (1995) Plant Cell, 7, pp. 1405-1419; 
Boisnard-Lorig, C., Colon-Carmona, A., Bauch, M., Hodge, S., Doerner, P., Bancharel, E., Dumas, C., Berger, F., Dynamic analyses of the expression of the HISTONE:YFP fusion protein in Arabidopsis show that syncytial endosperm is divided in mitotic domains (2001) Plant Cell, 13, pp. 495-509; 
Boniotti, M.B., Gutierrez, C., A cell-cycle-regulated kinase activity phosphorylates plant retinoblastoma protein and contains, in Arabidopsis, a CDKA/cyclin D complex (2001) Plant J., 28, pp. 341-350; 
Boruc, J., Van den Daele, H., Hollunder, J., Rombauts, S., Mylle, E., Hilson, P., Inz{\'e}, D., Russinova, E., Functional modules in the Arabidopsis core cell cycle binary proteinprotein interaction network (2010) Plant Cell, 22, pp. 1264-1280; 
Brady, S.M., Orlando, D.A., Lee, J.Y., Wang, J.Y., Koch, J., Dinneny, J.R., Mace, D., Benfey, P.N., A high-resolution root spatiotemporal map reveals dominant expression patterns (2007) Science, 318, pp. 801-806; 
Casimiro, I., Beeckman, T., Graham, N., Bhalerao, R., Zhang, H., Casero, P., Sandberg, G., Bennett, M.J., Dissecting Arabidopsis lateral root development (2003) Trends Plant Sci., 8, pp. 165-171; 
Casimiro, I., Marchant, A., Bhalerao, R.P., Beeckman, T., Dhooge, S., Swarup, R., Graham, N., Bennett, M., Auxin transport promotes Arabidopsis lateral root initiation (2001) Plant Cell, 13, pp. 843-852; 
Chevray, P.M., Nathans, D., Protein interaction cloning in yeast: Identification of mammalian proteins that react with the leucine zipper of Jun (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 5789-5793; 
Churchman, M.L., SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana (2006) Plant Cell, 18, pp. 3145-3157; 
Cleary, A., Fowke, L., Wang, H., John, P., The effect of ICK1, a plant cyclin-dependent kinase inhibitor, on mitosis in living plant cells (2002) Plant Cell Rep., 20, pp. 814-820; 
Clough, S.J., Bent, A.F., Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana (1998) Plant J., 16, pp. 735-743; 
Cockcroft, C.E., den Boer, B.G., Healy, J.M., Murray, J.A.H., Cyclin D control of growth rate in plants (2000) Nature, 405, pp. 575-579; 
de Smet, I., Vanneste, S., Inz{\'e}, D., Beeckman, T., Lateral root initiation or the birth of a new meristem (2006) Plant Mol. Biol., 60, pp. 871-887; 
de Smet, I., Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis (2007) Development, 134, pp. 681-690; 
de Smet, I., Bimodular auxin response controls organogenesis (2010) Proc. Natl. Acad. Sci. USA, 107, pp. 2705-2710; 
de Veylder, L., Beeckman, T., Beemster, G.T., de Almeida Engler, J., Ormenese, S., Maes, S., Naudts, M., Inz{\'e}, D., Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor (2002) EMBO J., 21, pp. 1360-1368; 
de Veylder, L., Beeckman, T., Beemster, G.T., Krols, L., Terras, F., Landrieu, I., van der Schueren, E., Inz{\'e}, D., Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis (2001) Plant Cell, 13, pp. 1653-1668; 
de Veylder, L., de Almeida Engler, J., Burssens, S., Manevski, A., Lescure, B., Van Montagu, M., Engler, G., Inz{\'e}, D., A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation (1999) Planta, 208, pp. 453-462; 
Dewitte, W., Murray, J.A.H., The plant cell cycle (2003) Annu. Rev. Plant Biol., 54, pp. 235-264; 
Dewitte, W., Riou-Khamlichi, C., Scofield, S., Healy, J.M., Jacqmard, A., Kilby, N.J., Murray, J.A.H., Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3 (2003) Plant Cell, 15, pp. 79-92; 
Dewitte, W., Scofield, S., Alcasabas, A.A., Maughan, S.C., Menges, M., Braun, N., Collins, C., Murray, J.A.H., Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses (2007) Proc. Natl. Acad. Sci. USA, 104, pp. 14537-14542; 
Dharmasiri, N., Dharmasiri, S., Weijers, D., Karunarathna, N., Jurgens, G., Estelle, M., AXL and AXR1 have redundant functions in RUB conjugation and growth and development in Arabidopsis (2007) Plant J., 52, pp. 114-123; 
Ditengou, F.A., Teale, W.D., Kochersperger, P., Flittner, K.A., Kneuper, I., van der Graaff, E., Nziengui, H., Palme, K., Mechanical induction of lateral root initiation in Arabidopsis thaliana (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 18818-18823; 
Dubrovsky, J.G., Rost, T.L., Col{\'o}n-Carmona, A., Doerner, P., Early primordium morphogenesis during lateral root initiation in Arabidopsis thaliana (2001) Planta, 214, pp. 30-36; 
Dubrovsky, J.G., Sauer, M., Napsucialy-Mendivil, S., Ivanchenko, M.G., Friml, J., Shishkova, S., Celenza, J., Benkov{\'a}, E., Auxin acts as a local morphogenetic trigger to specify lateral root founder cells (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 8790-8794; 
Duttweiler, H.M., A highly sensitive and non-lethal beta-galactosidase plate assay for yeast (1996) Trends Genet., 12, pp. 340-341; 
Ebel, C., Mariconti, L., Gruissem, W., Plant retinoblastoma homologues control nuclear proliferation in the female gametophyte (2004) Nature, 429, pp. 776-780; 
Fukaki, H., Tameda, S., Masuda, H., Tasaka, M., Lateral root formation is blocked by a gain-of-function mutation in the SOLITARY-ROOT/IAA14 gene of Arabidopsis (2002) Plant J., 29, pp. 153-168; 
Fukaki, H., Tasaka, M., Hormone interactions during lateral root formation (2009) Plant Mol. Biol., 69, pp. 437-449; 
Healy, J.M., Menges, M., Doonan, J.H., Murray, J.A.H., The Arabidopsis D-type cyclins CycD2 and CycD3 both interact in vivo with the PSTAIRE cyclin-dependent kinase Cdc2a but are differentially controlled (2001) J. Biol. Chem., 276, pp. 7041-7047; 
Himanen, K., Boucheron, E., Vanneste, S., de Almeida Engler, J., Inz{\'e}, D., Beeckman, T., Auxin-mediated cell cycle activation during early lateral root initiation (2002) Plant Cell, 14, pp. 2339-2351; 
Himanen, K., Vuylsteke, M., Vanneste, S., Vercruysse, S., Boucheron, E., Alard, P., Chriqui, D., Beeckman, T., Transcript profiling of early lateral root initiation (2004) Proc. Natl. Acad. Sci. USA, 101, pp. 5146-5151; 
Hirano, H., Harashima, H., Shinmyo, A., Sekine, M., Arabidopsis RETINOBLASTOMA-RELATED PROTEIN 1 is involved in G1 phase cell cycle arrest caused by sucrose starvation (2008) Plant Mol. Biol., 66, pp. 259-275; 
Huntley, R., The maize retinoblastoma protein homologue ZmRb-1 is regulated during leaf development and displays conserved interactions with G1/S regulators and plant cyclin D (CycD) proteins (1998) Plant Mol. Biol., 37, pp. 155-169; 
Inz{\'e}, D., De Veylder, L., Cell cycle regulation in plant development (2006) Annu. Rev. Genet., 40, pp. 77-105; 
Jakoby, M.J., Weinl, C., Pusch, S., Kuijt, S.J., Merkle, T., Dissmeyer, N., Schnittger, A., Analysis of the subcellular localization, function, and proteolytic control of the Arabidopsis cyclin-dependent kinase inhibitor ICK1/KRP1 (2006) Plant Physiol., 141, pp. 1293-1305; 
James, P., Halladay, J., Craig, E.A., Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast (1996) Genetics, 144, pp. 1425-1436; 
Jasinski, S., Riou-Khamlichi, C., Roche, O., Perennes, C., Bergounioux, C., Glab, N., The CDK inhibitor NtKIS1a is involved in plant development, endoreduplication and restores normal development of cyclin D3;1-overexpressing plants (2002) J. Cell Sci., 115, pp. 973-982; 
Karimi, M., Depicker, A., Hilson, P., Recombinational cloning with plant gateway vectors (2007) Plant Physiol., 145, pp. 1144-1154; 
Kohalmi, S.E., Nowak, J., Crosby, W.L., The yeast twohybrid system (1997) Differentially Expressed Genes in Plants: A Bench Manual, pp. 63-82. , In, E. Hansen and G. Harper, eds (London: Taylor \& Francis); 
Laskowski, M., Grieneisen, V.A., Hofhuis, H., Hove, C.A., Hogeweg, P., Mar{\'e}e, A.F., Scheres, B., Root system architecture from coupling cell shape to auxin transport (2008) PLoS Biol., 6, pp. e307; 
Laskowski, M.J., Williams, M.E., Nusbaum, H.C., Sussex, I.M., Formation of lateral root meristems is a two-stage process (1995) Development, 121, pp. 3303-3310; 
Lechner, E., Xie, D., Grava, S., Pigaglio, E., Planchais, S., Murray, J.A.H., Parmentier, Y., Genschik, P., The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects (2002) J. Biol. Chem., 277, pp. 50069-50080; 
Lincoln, C., Britton, J.H., Estelle, M., Growth and development of the axr1 mutants of Arabidopsis (1990) Plant Cell, 2, pp. 1071-1080; 
L{\'o}pez-Bucio, J., Cruz-Ram{\'\i}rez, A., Herrera-Estrella, L., The role of nutrient availability in regulating root architecture (2003) Curr. Opin. Plant Biol., 6, pp. 280-287; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated Arabidopsis plant root cell segmentation based on SVM classification and region merging (2009) Comput. Biol. Med., 39, pp. 785-793; 
Masubelele, N.H., Dewitte, W., Menges, M., Maughan, S., Collins, C., Huntley, R., Nieuwland, J., Murray, J.A.H., D-type cyclins activate division in the root apex to promote seed germination in Arabidopsis (2005) Proc. Natl. Acad. Sci. USA, 102, pp. 15694-15699; 
Menges, M., de Jager, S.M., Gruissem, W., Murray, J.A.H., Global analysis of the core cell cycle regulators of Arabidopsis identifies novel genes, reveals multiple and highly specific profiles of expression and provides a coherent model for plant cell cycle control (2005) Plant J., 41, pp. 546-566; 
Menges, M., Samland, A.K., Planchais, S., Murray, J.A.H., The D-type cyclin CYCD3;1 is limiting for the G1-to-S-phase transition in Arabidopsis (2006) Plant Cell, 18, pp. 893-906; 
Minet, M., Dufour, M.E., Lacroute, F.B., Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs (1992) Plant J., 2, pp. 417-422; 
Moreno-Risueno, M.A., Van Norman, J.M., Moreno, A., Zhang, J., Ahnert, S.E., Benfey, P.N., Oscillating gene expression determines competence for periodic Arabidopsis root branching (2010) Science, 329, pp. 1306-1311; 
Nieuwland, J., Maughan, S., Dewitte, W., Scofield, S., Sanz, L., Murray, J.A.H., The D-type cyclin CYCD4;1 modulates lateral root density in Arabidopsis by affecting the basal meristem region (2009) Proc. Natl. Acad. Sci. USA, 106, pp. 22528-22533; 
Okushima, Y., Fukaki, H., Onoda, M., Theologis, A., Tasaka, M., ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis (2007) Plant Cell, 19, pp. 118-130; 
P{\'e}ret, B., De Rybel, B., Casimiro, I., Benkov{\'a}, E., Swarup, R., Laplaze, L., Beeckman, T., Bennett, M.J., Arabidopsis lateral root development: An emerging story (2009) Trends Plant Sci., 14, pp. 399-408; 
Petersson, S.V., Johansson, A.I., Kowalczyk, M., Makoveychuk, A., Wang, J.Y., Moritz, T., Grebe, M., Ljung, K., An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis (2009) Plant Cell, 21, pp. 1659-1668; 
Petroski, M.D., Deshaies, R.J., Function and regulation of cullin-RING ubiquitin ligases (2005) Nat. Rev. Mol. Cell Biol., 6, pp. 9-20; 
Planchais, S., Samland, A.K., Murray, J.A.H., Differential stability of Arabidopsis D-type cyclins: CYCD3;1 is a highly unstable protein degraded by a proteasome-dependent mechanism (2004) Plant J., 38, pp. 616-625; 
Qi, R., John, P.C., Expression of genomic AtCYCD2;1 in Arabidopsis induces cell division at smaller cell sizes: Implications for the control of plant growth (2007) Plant Physiol., 144, pp. 1587-1597; 
Ren, H., Santner, A., del Pozo, J.C., Murray, J.A.H., Estelle, M., Degradation of the cyclin-dependent kinase inhibitor KRP1 is regulated by two different ubiquitin E3 ligases (2008) Plant J., 53, pp. 705-716; 
Richter, G.L., Monshausen, G.B., Krol, A., Gilroy, S., Mechanical stimuli modulate lateral root organogenesis (2009) Plant Physiol., 151, pp. 1855-1866; 
Riou-Khamlichi, C., Huntley, R., Jacqmard, A., Murray, J.A.H., Cytokinin activation of Arabidopsis cell division through a D-type cyclin (1999) Science, 283, pp. 1541-1544; 
Riou-Khamlichi, C., Menges, M., Healy, J.M., Murray, J.A.H., Sugar control of the plant cell cycle: Differential regulation of Arabidopsis D-type cyclin gene expression (2000) Mol. Cell. Biol., 20, pp. 4513-4521; 
Sabatini, S., Beis, D., Wolkenfelt, H., Murfett, J., Guilfoyle, T., Malamy, J., Benfey, P., Scheres, B., An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root (1999) Cell, 99, pp. 463-472; 
Schnittger, A., Sch{\"o}binger, U., Bouyer, D., Weinl, C., Stierhof, Y.D., H{\"u}lskamp, M., Ectopic D-type cyclin expression induces not only DNA replication but also cell division in Arabidopsis trichomes (2002) Proc. Natl. Acad. Sci. USA, 99, pp. 6410-6415; 
van Leene, J., Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana (2010) Mol. Syst. Biol., 6, p. 397; 
Vanneste, S., Cell cycle progression in the pericycle is not sufficient for SOLITARY ROOT/IAA14-mediated lateral root initiation in Arabidopsis thaliana (2005) Plant Cell, 17, pp. 3035-3050; 
Verkest, A., Manes, C.L., Vercruysse, S., Maes, S., van der Schueren, E., Beeckman, T., Genschik, P., De Veylder, L., The cyclin-dependent kinase inhibitor KRP2 controls the onset of the endoreduplication cycle during Arabidopsis leaf development through inhibition of mitotic CDKA;1 kinase complexes (2005) Plant Cell, 17, pp. 1723-1736; 
Verkest, A., Weinl, C., Inz{\'e}, D., De Veylder, L., Schnittger, A., Switching the cell cycle. Kip-related proteins in plant cell cycle control (2005) Plant Physiol., 139, pp. 1099-1106; 
Wang, H., Qi, Q., Schorr, P., Cutler, A.J., Crosby, W.L., Fowke, L.C., ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid (1998) Plant J., 15, pp. 501-510; 
Wildwater, M., Campilho, A., Perez-Perez, J.M., Heidstra, R., Blilou, I., Korthout, H., Chatterjee, J., Scheres, B., The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots (2005) Cell, 123, pp. 1337-1349; 
Willemse, J., Kulikova, O., de Jong, H., Bisseling, T., A new whole-mount DNA quantification method and the analysis of nuclear DNA content in the stem-cell niche of Arabidopsis roots (2008) Plant J., 55, pp. 886-894; 
Winter, D., Vinegar, B., Nahal, H., Ammar, R., Wilson, G.V., Provart, N.J., An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets (2007) PLoS ONE, 2, pp. e718; 
Zhou, Y., Fowke, L.C., Wang, H., Plant CDK inhibitors: Studies of interactions with cell cycle regulators in the yeast twohybrid system and functional comparisons in transgenic Arabidopsis plants (2002) Plant Cell Rep., 20, pp. 967-975; 
Zhou, Y., Niu, H., Brandizzi, F., Fowke, L.C., Wang, H., Molecular control of nuclear and subnuclear targeting of the plant CDK inhibitor ICK1 and ICK1-mediated nuclear transport of CDKA (2006) Plant Mol. Biol., 62, pp. 261-278; 
Zhou, Y., Wang, H., Gilmer, S., Whitwill, S., Fowke, L.C., Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana (2003) Planta, 216, pp. 604-613
},
	month = {2011///},
	pages = {641 - 660},
	abstract = {The integration of cell division in root growth and development requires mediation of developmental and physiological signals through regulation of cyclin-dependent kinase activity. Cells within the pericycle form de novo lateral root meristems, and D-type cyclins (CYCD), as regulators of the G                        1-to-S phase cell cycle transition, are anticipated to play a role. Here, we show that the D-type cyclin protein CYCD2;1 is nuclear in Arabidopsis thaliana root cells, with the highest concentration in apical and lateral meristems. Loss of CYCD2;1 has a marginal effect on unstimulated lateral root density, but CYCD2;1 is rate-limiting for the response to low levels of exogenous auxin. However, while CYCD2;1 expression requires sucrose, it does not respond to auxin. The protein Inhibitor-Interactor of CDK/Kip Related Protein2 (ICK2/KRP2), which interacts with CYCD2;1, inhibits lateral root formation, and ick2/krp2 mutants show increased lateral root density. ICK2/ KRP2 can modulate the nuclear levels of CYCD2;1, and since auxin reduces ICK2/KRP2 protein levels, it affects both activity and cellular distribution of CYCD2;1. Hence, as ICK2/KRP2 levels decrease, the increase in lateral root density depends on CYCD2;1, irrespective of ICK2/CYCD2;1 nuclear localization. We propose that ICK2/KRP2 restrains root ramification by maintaining CYCD2;1 inactive and that this modulates pericycle responses to auxin fluctuations. {\textcopyright} 2011 American Society of Plant Biologists.
},
	keywords = {Arabidopsis, Arabidopsis protein, Arabidopsis Proteins, Arabidopsis thaliana, article, ATP dependent 26S protease, cell cycle, cell cycle protein, Cell Cycle Proteins, cell nucleus, CycD2 protein, Arabidopsis, cycline, Cyclins, gene expression regulation, Gene Expression Regulation, Plant, genetics, growth, development and aging, ICK2 protein, Arabidopsis, indoleacetic acid derivative, Indoleacetic Acids, metabolism, mutation, plant root, Plant roots, proteasome, Proteasome Endopeptidase Complex},
	isbn = {10404651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953123085\&partnerID=40\&md5=f1355f64db2bcf9f2d881104f23005eb},
	author = {Sanz, L. and Dewitte, W. and Forzani, C. and Patell, F. and Nieuwland, J. and Wen, B. and Quelhas, P. and de Jager, S. and Titmus, C. and Campilho, A. and Ren, H. and Estelle, M. and Wang, H. and Murray, J.A.H.}
}
@book {2222,
	title = {Arabidopsis thaliana automatic cell file detection and cell length estimation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6754 LNCS},
	year = {2011},
	note = {Conference code: 85546Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21596-4_1Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB - Divis{\~a}o de Sinal e Imagem, Instituto de Engenharia Biom{\'e}dica, Porto, PortugalReferences: Terpstra, I., Heidstra, R., Stem cells: The root of all cells (2009) Semin. Cell Dev. Biol., 20 (9), pp. 1089-1096;Bennett, T., Scheres, B., Root development-two meristems for the price of one? (2010) Curr. Top Dev. Bio., 91, pp. 67-102; 
De Smet, I., Tetsumura, T., De Rybel, B., Frey, N.F., Laplaze, L., Casimiro, I., Swarup, R., Beeckman, T., Auxin-dependent regulation of lateral root positioning in the basal meristem of arabidopsis (2007) Development, 134 (4), pp. 681-690; 
Nieuwland, J., Maughan, S., Dewitte, W., Scofield, S., Sanz, L., Murray, J.A., The d-type cyclin cycd4;1 modulates lateral root density in arabidopsis by affecting the basal meristem region (2009) Proc. Natl. Acad. Sci. U S A, 106 (52), pp. 22528-22533; 
Vanstraelen, M., Baloban, M., Da Ines, O., Cultrone, A., Lammens, T., Boudolf, V., Brown, S.C., Kondorosi, E., Apc/c-ccs52a complexes control meristem maintenance in the arabidopsis root (2009) Proc. Natl. Acad. Sci. U S A, 106 (28), pp. 11806-11811; 
Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res., 119, pp. 85-93; 
Immink, R., Gadella, T., Ferrario, S., Busscher, M., Angenent, G., Analysis of mads box protein-protein interactions in living plant cells (2002) Proc. Natl. Acad. Sci. USA, 99, pp. 2416-2421; 
Willemse, J., Kulikova, O., Jong, H., Bisseling, T., A new whole-mount dna quantification method and the analysis of nuclear dna content in the stem-cell niche of arabidopsis roots (2008) The Plant Journal, 10, pp. 1365-1374; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Comp. in Biology and Medicine, 39, pp. 785-793; 
Marcuzzo, M., Guichard, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Araujo, H., Mendon{\c c}a, A.M., Pinho, A.J., Torres, M.I. (eds.) IbPRIA 2009. Springer, Heidelberg; 
Kovesi, P., Symmetry and asymmetry from local phase Proc. of the 10th Australian Joint Conf. on Artificial Intelligence (December 1997); 
Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Transactions on Systems, Man, and Cybernetics, SMC-9 (1), pp. 62-66
},
	month = {2011///},
	pages = {1 - 11},
	address = {Burnaby, BC},
	abstract = {In plant development biology, the study of the structure of the plant{\textquoteright}s root is fundamental for the understanding of the regulation and interrelationships of cell division and cellular differentiation. This is based on the high connection between cell length and progression of cell differentiation and the nuclear state. However, the need to analyse a large amount of images from many replicate roots to obtain reliable measurements motivates the development of automatic tools for root structure analysis. We present a novel automatic approach to detect cell files, the main structure in plant roots, and extract the length of the cells in those files. This approach is based on the detection of local cell file characteristic symmetry using a wavelet based image symmetry measure. The resulting detection enables the automatic extraction of important data on the plant development stage and of characteristics for individual cells. Furthermore, the approach presented reduces in more than 90\% the time required for the analysis of each root, improving the work of the biologist and allowing the increase of the amount of data to be analysed for each experimental condition. While our approach is fully automatic a user verification and editing stage is provided so that any existing errors may be corrected. Given five test images it was observed that user did not correct more than 20\% of all automatically detected structure, while taking no more than 10\% of manual analysis time to do so. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Automatic extraction, Automatic tools, Bio-imaging, BioImaging, cell differentiation, Cell divisions, Cell lengths, cell proliferation, Cells, Cellular differentiation, Experimental conditions, Image analysis, image symmetry, Individual cells, Main structure, Manual analysis, Nuclear state, Plant development, Plant roots, Plants (botany), Reliable measurement, Root structure, Test images, User verification, Wavelet analysis, Wavelet-based images},
	isbn = {03029743 (ISSN); 9783642215957 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960337749\&partnerID=40\&md5=ef49700082788511730e38d4eda9a566},
	author = {Quelhas, P. and Nieuwland, J. and Dewitte, W. and Mendon{\c c}a, A.M. and Murray, J. and Campilho, A.}
}
@conference {2226,
	title = {Automatic segmentation of chromatographic images for region of interest delineation},
	booktitle = {Progress in Biomedical Optics and Imaging - Proceedings of SPIEProgr. Biomed. Opt. Imaging Proc. SPIE},
	series = {Medical Imaging 2011: Image Processing},
	volume = {7962},
	year = {2011},
	note = {Conference code: 84913Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusArt. No.: 79623Bdoi: 10.1117/12.877671Language of Original Document: EnglishCorrespondence Address: Mendon{\c c}a, A. M.; Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal; email: amendon@fe.up.ptReferences: Zarate, Y., Hopkin, R., Fabry{\textquoteright}s disease (2008) The Lancet, 372 (9647), pp. 1427-1435;Gerasimov, A.V., Use of the Software Processing of Scanned Chromatogram Images in Quantitative Planar Chromatography (2004) J. of Anal Chem., 59 (4), pp. 348-353; 
Bajla, I., Hollander, I., Fluch, S., Burg, K., Kollar, M., An alternative method for electrophoretic gel image analysis in the GelMaster software (2005) Comput Methods Programs Biomed, 77 (3), pp. 209-231; 
Sousa, A.V., Mendonca, A.M., Campilho, A., Chromatographic pattern classification (2008) IEEE Trans on Biomedical Engineering, 55 (6), pp. 1687-1696; 
Bajla, I., Rubl{\'\i}k, F., Arendack{\'a}, B., Farka{\v s}, I., Horni{\v s}ov{\'a}, K., {\v S}tolc, S., Witkovsk{\'y}, V., Segmentation and supervised classification of image objects in Epo doping-control (2009) Machine Vision and Applications, 20 (4), pp. 243-259; 
Sousa, A.V., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic lane and band detection in images of Thin-Layer Chromatography (2004) LNCS, 3212, pp. 158-216. , Springer; 
Dempster, A., Laird, N., Rubin, D., Maximum Likelihood from incomplete data via the EM algorithm (1977) J. of the Royal Statistical Society. Series B, 39 (1), pp. 1-38; 
Heijden, F., Duin, R.P.W., Ridder, D., Tax, D.M.J., (2004) Classification, Parameter Estimation and State Estimation - An Engineering Approach Using Matlab, , John Wiley \& Sons
Sponsors: The Society of Photo-Optical Instrumentation Engineers (SPIE); Dynasil Corporation/RMD Research; American Association of Physicists in Medicine (AAPM); DQE Instruments, Inc.; Ocean Thin Films, Inc.},
	month = {2011///},
	address = {Lake Buena Vista, FL},
	abstract = {This paper describes a segmentation method for automating the region of interest (ROI) delineation in chromatographic images, thus allowing the definition of the image area that contains the fundamental information for further processing while excluding the frame of the chromatographic plate that does not contain relevant data for disease identification. This is the first component of a screening tool for Fabry disease, which will be based on the automatic analysis of the chromatographic patterns extracted from the image ROI. Image segmentation is performed in two phases, where each individual pixel is finally considered as frame or ROI. In the first phase, an unsupervised learning method is used for classifying image pixels into three classes: frame, ROI or unknown. In the second phase, distance features are used for deciding which class the unknown pixels belong to. The segmentation result is post-processed using a sequence of morphological operators in order to obtain the final ROI rectangular area. The proposed methodology was successfully evaluated in a dataset of 41 chromatographic images. {\textcopyright} 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).},
	keywords = {Automatic analysis, Automatic segmentations, Chromatographic images, Chromatographic plates, Chromatography, Data sets, Distance feature, Fabry disease, Image pixels, Image segmentation, Imaging systems, Medical imaging, Morphological operator, Pixels, Rectangular area, Region of interest, Region of interest delineation, Screening tool, Second phase, Segmentation methods, Segmentation results, Unsupervised learning, Unsupervised learning method},
	isbn = {16057422 (ISSN); 9780819485045 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79958001297\&partnerID=40\&md5=e679e7314308e03c9042ba3909310a7c},
	author = {Mendon{\c c}a, A.M. and Sousa, A.V. and S{\'a}-Miranda, M.C. and Campilho, A.C.}
}
@book {2221,
	title = {Classification-based segmentation of the region of interest in chromatographic images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6754 LNCS},
	year = {2011},
	note = {Conference code: 85546Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21596-4_8Language of Original Document: EnglishCorrespondence Address: Sousa, A.V.; Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: asousa@eu.ipp.ptReferences: Zarate, Y., Hopkin, R., Fabry{\textquoteright}s Disease (2008) The Lancet, 372 (9647), pp. 1427-1435;Linthorst, G.E., Vedder, A., Aerts, J.M., Hollak, C.E., Screening for Fabry Disease Using Whole Blood Spots Fails to Identify One-third of Female Carriers (2005) Clinica Chimica Acta, 353 (1-2), pp. 201-203; 
Eng, C.M., Germain, D.P., Banikazemi, M., Warnock, D.G., Wanner, C., Hopkin, R.J., Bultas, J., Wilcox, W.R., Fabry Disease: Guidelines for the Evaluation and Management of Multi-organ System Involvement (2006) Genetics in Medicine, 8 (9), pp. 539-548; 
Gerasimov, A.V., Use of the Software Processing of Scanned Chromatogram Images in Quantitative Planar Chromatography (2004) J. of Anal. Chem., 59 (4), pp. 348-353; 
Bajla, I., Hollander, I., Fluch, S., Burg, K., Kollar, M., An Alternative Method for Electrophoretic Gel Image Analysis in the Gelmaster Software (2005) Comput. Methods Programs Biomed., 77 (3), pp. 209-231; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Chromatographic Pattern Classification (2008) IEEE Trans. on Biomedical Engineering, 55 (6), pp. 1687-1696; 
Bajla, I., Rubl{\'\i}k, F., Arendack{\'a}, B., Farka{\v s}, I., Horni{\v s}ov{\'a}, K., {\v S}tolc, S., Witkovsk{\'y}, V., Segmentation and Supervised Classification of Image Objects in Epo Doping-control (2009) Machine Vision and Applications, 20 (4), pp. 243-259; 
Sousa, A.V., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic Lane and Band Detection in Images of Thin Layer Chromatography (2004) LNCS, 3212, pp. 158-165. , Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. Springer, Heidelberg; 
Mendon{\c c}a, A.M., Sousa, A.V., S{\'a}-Miranda, M.C., Campilho, A., Automatic segmentation of chromatographic images for region of interest delineation (2011) SPIE Medical Imaging; 
Dempster, A., Laird, N., Rubin, D., Maximum Likelihood from Incomplete Data via the EM Algorithm (1997) J. of the Royal Statistical Society. Series B, 39 (1), pp. 1-38; 
Heijden, F., Robert, P.W.D., Ridder, D., Tax, D.M.J., (2004) Classification, Parameter Estimation and State Estimation, , John Wiley \& Sons, Chichester
},
	month = {2011///},
	pages = {68 - 78},
	address = {Burnaby, BC},
	abstract = {This paper proposes a classification-based method for automating the segmentation of the region of interest (ROI) in digital images of chromatographic plates. Image segmentation is performed in two phases. In the first phase an unsupervised learning method classifies the image pixels into three classes: frame, ROI or unknown. In the second phase, distance features calculated for the members of the three classes are used for deciding on the new label, ROI or frame, for each individual connected segment previously classified as unknown.The segmentation result is post-processed using a sequence of morphological operators before obtaining the final ROI rectangular area. The proposed methodology, which is the initial step for the development of a screening tool for Fabry disease, was successfully evaluated in a dataset of 58 chromatographic images. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Chromatographic images, Chromatographic plates, Chromatography, Data sets, Digital image, Distance feature, Fabry disease, Image analysis, Image pixels, Image segmentation, Morphological operator, Rectangular area, Region of interest, Region of interest delineation, Screening tool, Second phase, Segmentation results, Unsupervised learning, Unsupervised learning method},
	isbn = {03029743 (ISSN); 9783642215957 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960331409\&partnerID=40\&md5=c248f56c8a7bda71832c4b569e32164b},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and S{\'a}-Miranda, M.C. and Campilho, A.}
}
@article {2236,
	title = {Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2010.11.005PubMed ID: 21056701Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; carbenicillin, 17230-86-3, 4697-36-3, 4800-94-6; cefazolin, 25953-19-9, 27164-46-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; penicillin G, 1406-05-9, 61-33-6; teicoplanin, 61036-62-2, 61036-64-4; tobramycin, 32986-56-4; vancomycin, 1404-90-6, 1404-93-9; Antimicrobial Cationic Peptides; Biocompatible Materials; Coated Materials, Biocompatible; Immobilized ProteinsReferences: Glinel, K., Jonas, A.M., Jouenne, T., Leprince, J., Galas, L., Huck, W.T., Antibacterial and antifouling polymer brushes incorporating antimicrobial peptide (2009) Bioconj Chem, 20 (1), pp. 71-77;Vila, J., Soriano, A., Mensa, J., Molecular basis of microbial adherence to prosthetic materials. Role of biofilms in prosthesis-associated infection (2008) Enferm Infecc Microbiol Clin, 26 (1), pp. 48-54; 
Hetrick, E.M., Schoenfisch, M.H., Reducing implant-related infections: Active release strategies (2006) Chemical Society Reviews, 35 (9), pp. 780-789. , DOI 10.1039/b515219b; 
Darouiche, R.O., Treatment of Infections Associated with Surgical Implants (2004) New England Journal of Medicine, 350 (14), pp. 1422-1429. , DOI 10.1056/NEJMra035415; 
Zhao, L., Chu, P.K., Zhang, Y., Wu, Z., Antibacterial coatings on titanium implants (2009) J Biomed Mater Res B Appl Biomater, 91 (1), pp. 470-480; 
Dee, K.C., Puleo, D.A., Bizios, R., (2002) An Introduction to Tissue-biomaterial Interactions, , Wiley-Liss New York; 
Subramani, K., Jung, R.E., Molenberg, A., Hammerle, C.H., Biofilm on dental implants: A review of the literature (2009) Int J Oral Maxillofac Implants, 24 (4), pp. 616-626; 
Fey, P.D., Olson, M.E., Current concepts in biofilm formation of Staphylococcus epidermidis (2010) Future Microbiol, 5 (6), pp. 917-933; 
De Carvalho, C.C., Biofilms: Recent developments on an old battle (2007) Recent Pat Biotechnol, 1 (1), pp. 49-57; 
Costerton, J.W., Stewart, P.S., Greenberg, E.P., Bacterial biofilms: A common cause of persistent infections (1999) Science, 284 (5418), pp. 1318-1322. , DOI 10.1126/science.284.5418.1318; 
Willcox, M.D., Hume, E.B., Aliwarga, Y., Kumar, N., Cole, N., A novel cationic-peptide coating for the prevention of microbial colonization on contact lenses (2008) J Appl Microbiol, 105 (6), pp. 1817-1825; 
Lewis, K., Riddle of biofilm resistance (2001) Antimicrobial Agents and Chemotherapy, 45 (4), pp. 999-1007. , DOI 10.1128/AAC.45.4.999-1007.2001; 
Monteiro, D.R., Gorup, L.F., Takamiya, A.S., Ruvollo-Filho, A.C., De Camargo, E.R., Barbosa, D.B., The growing importance of materials that prevent microbial adhesion: Antimicrobial effect of medical devices containing silver (2009) Int J Antimicrob Agents, 34 (2), pp. 103-110; 
Gooderham, W.J., Bains, M., McPhee, J.B., Wiegand, I., Hancock, R.E., Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation, and swarming motility of PsrA in Pseudomonas aeruginosa (2008) J Bacteriol, 190 (16), pp. 5624-5634; 
Bagge, N., Schuster, M., Hentzer, M., Ciofu, O., Givskov, M., Greenberg, E.P., Hoiby, N., Pseudomonas aeruginosa Biofilms Exposed to Imipenem Exhibit Changes in Global Gene Expression and β-Lactamase and Alginate Production (2004) Antimicrobial Agents and Chemotherapy, 48 (4), pp. 1175-1187. , DOI 10.1128/AAC.48.4.1175-1187.2004; 
Ahmed, N.A., Petersen, F.C., Scheie, A.A., AI-2/LuxS is involved in increased biofilm formation by Streptococcus intermedius in the presence of antibiotics (2009) Antimicrob Agents Chemother, 53 (10), pp. 4258-4263; 
Hoffman, L.R., D{\textquoteright}Argenio, D.A., MacCoss, M.J., Zhang, Z., Jones, R.A., Miller, S.I., Aminoglycoside antibiotics induce bacterial biofilm formation (2005) Nature, 436 (7054), pp. 1171-1175. , DOI 10.1038/nature03912; 
Harbers, G.M., Emoto, K., Greef, C., Metzger, S.W., Woodward, H.N., Mascali, J.J., Grainger, D.W., Lochhead, M.J., Functionalized poly(ethylene glycol)-based bioassay surface chemistry that facilitates bio-immobilization and inhibits nonspecific protein, bacterial, and mammalian cell adhesion (2007) Chemistry of Materials, 19 (18), pp. 4405-4414. , DOI 10.1021/cm070509u; 
Chapman, R.G., Ostuni, E., Liang, M.N., Meluleni, G., Kim, E., Yan, L., Pier, G., Whitesides, G.M., Polymeric thin films that resist the adsorption of proteins and the adhesion of bacteria (2001) Langmuir, 17 (4), pp. 1225-1233. , DOI 10.1021/la001222d; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res A, 89 (3), pp. 642-653; 
Chung, D., Papadakis, S.E., Yam, K.L., Evaluation of a polymer coating containing triclosan as the antimicrobial layer for packaging materials (2003) International Journal of Food Science and Technology, 38 (2), pp. 165-169. , DOI 10.1046/j.1365-2621.2003.00657.x; 
Leung, D., Spratt, D.A., Pratten, J., Gulabivala, K., Mordan, N.J., Young, A.M., Chlorhexidine-releasing methacrylate dental composite materials (2005) Biomaterials, 26 (34), pp. 7145-7153. , DOI 10.1016/j.biomaterials.2005.05.014, PII S0142961205003881; 
Ravikumar, T., Murata, H., Koepsel, R.R., Russell, A.J., Surface-active antifungal polyquaternary amine (2006) Biomacromolecules, 7 (10), pp. 2762-2769. , DOI 10.1021/bm060476w; 
Bach, A., Darby, D., Bottiger, B., Bohrer, H., Motsch, J., Martin, E., Retention of the antibiotic teicoplanin on a hydromer-coated central venous catheter to prevent bacterial colonization in postoperative surgical patients (1996) Intensive Care Medicine, 22 (10), pp. 1066-1069. , DOI 10.1007/s001340050214; 
Jose, B., Antoci Jr., V., Zeiger, A.R., Wickstrom, E., Hickok, N.J., Vancomycin covalently bonded to titanium beads kills Staphylococcus aureus (2005) Chemistry and Biology, 12 (9), pp. 1041-1048. , DOI 10.1016/j.chembiol.2005.06.013, PII S1074552105002309; 
Kilic, D., Agalar, C., Ozturk, E., Denkbas, E.B., Cime, A., Agalar, F., Antimicrobial activity of cefazolin-impregnated mesh grafts (2007) ANZ Journal of Surgery, 77 (4), pp. 256-260. , DOI 10.1111/j.1445-2197.2007.04029.x; 
Gallardo-Moreno, A.M., Pacha-Olivenza, M.A., Saldana, L., Perez-Giraldo, C., Bruque, J.M., Vilaboa, N., In vitro biocompatibility and bacterial adhesion of physico-chemically modified Ti6Al4V surface by means of UV irradiation (2009) Acta Biomater, 5 (1), pp. 181-192; 
Jansen, B., Kohnen, W., Prevention of biofilm formation by polymer modification (1995) Journal of Industrial Microbiology, 15 (4), pp. 391-396. , DOI 10.1007/BF01569996; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly (hydroxyethylmethacrylate)) surfaces (2003) Journal of Biomaterials Science, Polymer Edition, 14 (5), pp. 439-455. , DOI 10.1163/156856203766652057; 
Zhang, F., Zhang, Z., Zhu, X., Kang, E.T., Neoh, K.G., Silk-functionalized titanium surfaces for enhancing osteoblast functions and reducing bacterial adhesion (2008) Biomaterials, 29 (36), pp. 4751-4759; 
Flemming, R.G., Capelli, C.C., Cooper, S.L., Proctor, R.A., Bacterial colonization of functionalized polyurethanes (2000) Biomaterials, 21 (3), pp. 273-281; 
Singla, A.K., Chawla, M., Chitosan: Some pharmaceutical and biological aspects - An update (2001) Journal of Pharmacy and Pharmacology, 53 (8), pp. 1047-1067. , DOI 10.1211/0022357011776441; 
Murata, H., Koepsel, R.R., Matyjaszewski, K., Russell, A.J., Permanent, non-leaching antibacterial surfaces-2: How high density cationic surfaces kill bacterial cells (2007) Biomaterials, 28 (32), pp. 4870-4879. , DOI 10.1016/j.biomaterials.2007.06.012, PII S0142961207004681; 
Aumsuwan, N., Danyus, R.C., Heinhorst, S., Urban, M.W., Attachment of ampicillin to expanded poly(tetrafluoroethylene): Surface reactions leading to inhibition of microbial growth (2008) Biomacromolecules, 9 (7), pp. 1712-1718; 
Cevher, E., Orhan, Z., Mulazimoglu, L., Sensoy, D., Alper, M., Yildiz, A., Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus with prepared microspheres (2006) Int J Pharm, 317 (2), pp. 127-135; 
Faber, C., Stallmann, H.P., Lyaruu, D.M., Joosten, U., Von Eiff, C., Van Nieuw Amerongen, A., Wuisman, P.I.J.M., Comparable efficacies of the antimicrobial peptide human lactoferrin 1-11 and gentamicin in a chronic methicillin-resistant Staphylococcus aureus osteomyelitis model (2005) Antimicrobial Agents and Chemotherapy, 49 (6), pp. 2438-2444. , DOI 10.1128/AAC.49.6.2438-2444.2005; 
Aumsuwan, N., Heinhorst, S., Urban, M.W., Antibacterial surfaces on expanded polytetrafluoroethylene; penicillin attachment (2007) Biomacromolecules, 8 (2), pp. 713-718. , DOI 10.1021/bm061050k; 
Palumbi, S.R., Humans as the world{\textquoteright}s greatest evolutionary force (2001) Science, 293 (5536), pp. 1786-1790. , DOI 10.1126/science.293.5536.1786; 
Andreu, D., Rivas, L., Animal antimicrobial peptides: An overview (1998) Biopolymers, 47 (6), pp. 415-433; 
Rivas, L., Luque-Ortega, J.R., Andreu, D., Amphibian antimicrobial peptides and Protozoa: Lessons from parasites (2009) Biochim Biophys Acta, 1788 (8), pp. 1570-1581; 
Gordon, Y.J., Romanowski, E.G., McDermott, A.M., Mini review: A review of antimicrobial peptides and their therapeutic potential as anti-infective drugs (2005) Current Eye Research, 30 (7), pp. 505-515. , DOI 10.1080/02713680590968637; 
Guani-Guerra, E., Santos-Mendoza, T., Lugo-Reyes, S.O., Teran, L.M., Antimicrobial peptides: General overview and clinical implications in human health and disease (2010) Clin Immunol, 135 (1), pp. 1-11; 
Perron, G.G., Zasloff, M., Bell, G., Experimental evolution of resistance to an antimicrobial peptide (2006) Proceedings of the Royal Society B: Biological Sciences, 273 (1583), pp. 251-256. , DOI 10.1098/rspb.2005.3301; 
Reddy, K.V.R., Yedery, R.D., Aranha, C., Antimicrobial peptides: Premises and promises (2004) International Journal of Antimicrobial Agents, 24 (6), pp. 536-547. , DOI 10.1016/j.ijantimicag.2004.09.005, PII S092485790400322X; 
Van {\textquoteright}T Hof, W., Veerman, E.C.I., Heimerhorst, E.J., Nieuw Amerongen, A.V., Antimicrobial peptides: Properties and applicability (2001) Biological Chemistry, 382 (4), pp. 597-619. , DOI 10.1515/BC.2001.072; 
Li, M., Cha, D.J., Lai, Y., Villaruz, A.E., Sturdevant, D.E., Otto, M., The antimicrobial peptide-sensing system aps of Staphylococcus aureus (2007) Molecular Microbiology, 66 (5), pp. 1136-1147. , DOI 10.1111/j.1365-2958.2007.05986.x; 
Li, M., Lai, Y., Villaruz, A.E., Cha, D.J., Sturdevant, D.E., Otto, M., Gram-positive three-component antimicrobial peptide-sensing system (2007) Proceedings of the National Academy of Sciences of the United States of America, 104 (22), pp. 9469-9474. , DOI 10.1073/pnas.0702159104; 
Otto, M., Bacterial sensing of antimicrobial peptides (2009) Contrib Microbiol, 16, pp. 136-149; 
Peschel, A., Vincent Collins, L., Staphylococcal resistance to antimicrobial peptides of mammalian and bacterial origin (2001) Peptides, 22 (10), pp. 1651-1659. , DOI 10.1016/S0196-9781(01)00500-9, PII S0196978101005009; 
Peschel, A., Jack, R.W., Otto, M., Collins, L.V., Staubitz, P., Nicholson, G., Kalbacher, H., Van Strijp, J.A.G., Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with L-lysine (2001) Journal of Experimental Medicine, 193 (9), pp. 1067-1076. , DOI 10.1084/jem.193.9.1067; 
Cao, M., Helmann, J.D., The Bacillus subtilis Extracytoplasmic-Function σ X Factor Regulates Modification of the Cell Envelope and Resistance to Cationic Antimicrobial Peptides (2004) Journal of Bacteriology, 186 (4), pp. 1136-1146. , DOI 10.1128/JB.186.4.1136-1146.2004; 
Clarke, S.R., Foster, S.J., IsdA protects Staphylococcus aureus against the bactericidal protease activity of apolactoferrin (2008) Infection and Immunity, 76 (4), pp. 1518-1526. , DOI 10.1128/IAI.01530-07; 
Vuong, C., Voyich, J.M., Fischer, E.R., Braughton, K.R., Whitney, A.R., DeLeo, F.R., Otto, M., Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system (2004) Cellular Microbiology, 6 (3), pp. 269-275. , DOI 10.1046/j.1462-5822.2004.00367.x; 
Zasloff, M., Antimicrobial peptides of multicellular organisms (2002) Nature, 415 (6870), pp. 389-395. , DOI 10.1038/415389a; 
Hancock, R.E.W., Peptide antibiotics (1997) Lancet, 349 (9049), pp. 418-422. , DOI 10.1016/S0140-6736(97)80051-7; 
Cho, W.-M., Joshi, B.P., Cho, H., Lee, K.-H., Design and synthesis of novel antibacterial peptide-resin conjugates (2007) Bioorganic and Medicinal Chemistry Letters, 17 (21), pp. 5772-5776. , DOI 10.1016/j.bmcl.2007.08.056, PII S0960894X0701013X; 
Statz, A.R., Park, J.P., Chongsiriwatana, N.P., Barron, A.E., Messersmith, P.B., Surface-immobilised antimicrobial peptoids (2008) Biofouling, 24 (6), pp. 439-448; 
Deslouches, B., Gonzalez, I.A., DeAlmeida, D., Islam, K., Steele, C., Montelaro, R.C., Mietzner, T.A., De novo-derived cationic antimicrobial peptide activity in a murine model of Pseudomonas aeruginosa bacteraemia (2007) Journal of Antimicrobial Chemotherapy, 60 (3), pp. 669-672. , DOI 10.1093/jac/dkm253; 
Novak, K.F., Diamond, W.J., Kirakodu, S., Peyyala, R., Anderson, K.W., Montelaro, R.C., Mietzner, T.A., Efficacy of the de novo-derived antimicrobial peptide WLBU2 against oral bacteria (2007) Antimicrobial Agents and Chemotherapy, 51 (5), pp. 1837-1839. , DOI 10.1128/AAC.00924-06; 
Deslouches, B., Islam, K., Craigo, J.K., Paranjape, S.M., Montelaro, R.C., Mietzner, T.A., Activity of the de novo engineered antimicrobial peptide WLBU2 against Pseudomonas aeruginosa in human serum and whole blood: Implications for systemic applications (2005) Antimicrobial Agents and Chemotherapy, 49 (8), pp. 3208-3216. , DOI 10.1128/AAC.49.8.3208-3216.2005; 
Deslouches, B., Phadke, S.M., Lazarevic, V., Cascio, M., Islam, K., Montelaro, R.C., Mietzner, T.A., De novo generation of cationic antimicrobial peptides: Influence of length and tryptophan substitution on antimicrobial activity (2005) Antimicrobial Agents and Chemotherapy, 49 (1), pp. 316-322. , DOI 10.1128/AAC.49.1.316-322.2005; 
Levin, M., Quint, P.A., Goldstein, B., Barton, P., Bradley, J.S., Shemie, S.D., Recombinant bactericidal/permeability-increasing protein (rBPI21) as adjunctive treatment for children with severe meningococcal sepsis: A randomised trial. rBPI21 Meningococcal Sepsis Study Group (2000) Lancet, 356 (9234), pp. 961-967; 
Giroir, B.P., Scannon, P.J., Levin, M., Bactericidal/permeability-increasing protein-lessons learned from the phase III, randomized, clinical trial of rBPI21 for adjunctive treatment of children with severe meningococcemia (2001) Crit Care Med, 29 (7 SUPPL.), pp. 130-135; 
Brogden, K.A., Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? (2005) Nature Reviews Microbiology, 3 (3), pp. 238-250. , DOI 10.1038/nrmicro1098; 
Bastos, M., Bai, G., Gomes, P., Andreu, D., Goormaghtigh, E., Prieto, M., Energetics and partition of two cecropin-melittin hybrid peptides to model membranes of different composition (2008) Biophys J, 94 (6), pp. 2128-2141; 
Melo, M.N., Ferre, R., Castanho, M.A., Antimicrobial peptides: Linking partition, activity and high membrane-bound concentrations (2009) Nat Rev Microbiol, 7 (3), pp. 245-250; 
Haynie, S.L., Crum, G.A., Doele, B.A., Antimicrobial activities of amphiphilic peptides covalently bonded to a water-insoluble resin (1995) Antimicrob Agents Chemother, 39 (2), pp. 301-307; 
Humblot, V., Yala, J.F., Thebault, P., Boukerma, K., Hequet, A., Berjeaud, J.M., The antibacterial activity of Magainin i immobilized onto mixed thiols self-assembled monolayers (2009) Biomaterials, 30 (21), pp. 3503-3512; 
Bagheri, M., Beyermann, M., Dathe, M., Immobilization reduces the activity of surface-bound cationic antimicrobial peptides with no influence upon the activity spectrum (2009) Antimicrob Agents Chemother, 53 (3), pp. 1132-1141; 
Gabriel, M., Nazmi, K., Veerman, E.C., Nieuw Amerongen, A.V., Zentner, A., Preparation of LL-37-grafted titanium surfaces with bactericidal activity (2006) Bioconj Chem, 17 (2), pp. 548-550; 
Hilpert, K., Elliott, M., Jenssen, H., Kindrachuk, J., Fjell, C.D., Korner, J., Screening and characterization of surface-tethered cationic peptides for antimicrobial activity (2009) Chem Biol, 16 (1), pp. 58-69; 
Batista, M.K.S., Gallemi, M., Adeva, A., Gomes, C.A.R., Gomes, P., Facile regioselective synthesis of a novel chitosan-pexiganan conjugate with potential interest for the treatment of infected skin lesions (2009) Synthetic Commun, 39 (7), pp. 1228-1240; 
Venter, J.C., Immobilized and insolubilized drugs, hormones, and neurotransmitters: Properties, mechanisms of action and applications (1982) Pharmacological Reviews, 34 (2), pp. 153-187; 
Endo, Y., Tani, T., Kodama, M., Antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber (1987) Appl Environ Microbiol, 53 (9), pp. 2050-2055; 
Chuang, H.F., Shukla, A., Loose, C.R., Hammond-Cunningham, P.T., Stephanopoulos, G., (2009) Structures Including Antimicrobial Peptides, , United States; 
Chen, R., Cole, N., Willcox, M.D., Park, J., Rasul, R., Carter, E., Synthesis, characterization and in vitro activity of a surface-attached antimicrobial cationic peptide (2009) Biofouling, 25 (6), pp. 517-524; 
Steven, M.D., Hotchkiss, J.H., Covalent immobilization of an antimicrobial peptide on poly(ethylene) film (2008) J Appl Polym Sci, 110 (5), pp. 2665-2670; 
Appendini, P., Hotchkiss, J.H., Surface modification of poly(styrene) by the attachment of an antimicrobial peptide (2001) J Appl Polym Sci, 81 (3), pp. 609-616; 
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) International Journal of Peptide and Protein Research, 35 (3), pp. 161-214; 
Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B., A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes (2002) Angewandte Chemie - International Edition, 41 (14), pp. 2596-2599. , DOI 10.1002/1521-3773(20020715)41: 14<2596::AID-ANIE2596>3.0.CO;2-4; 
Abad, J.M., Velez, M., Santamaria, C., Guisan, J.M., Matheus, P.R., Vazquez, L., Gazaryan, I., Fernandez, V.M., Immobilization of peroxidase glycoprotein on gold electrodes modified with mixed epoxy-boronic acid monolayers (2002) Journal of the American Chemical Society, 124 (43), pp. 12845-12853. , DOI 10.1021/ja026658p; 
Lee, M.-R., Shin, I., Fabrication of chemical microarrays by efficient immobilization of hydrazide-linked substances on epoxide-coated glass surfaces (2005) Angewandte Chemie - International Edition, 44 (19), pp. 2881-2884. , DOI 10.1002/anie.200462720; 
Hudalla, G.A., Murphy, W.L., Immobilization of peptides with distinct biological activities onto stem cell culture substrates using orthogonal chemistries (2010) Langmuir, 26 (9), pp. 6449-6456; 
Cole, N., Hume, E.B., Vijay, A.K., Sankaridurg, P., Kumar, N., Willcox, M.D., In vivo performance of melimine as an antimicrobial coating for contact lenses in models of CLARE and CLPU (2010) Invest Ophthalmol Vis Sci, 51 (1), pp. 390-395
},
	month = {2011///},
	pages = {1431 - 1440},
	abstract = {Bacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs{\textquoteright} potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and long-term stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices. {\textcopyright} 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Amino Acid Sequence, amoxicillin, ampicillin, Animals, antibiotic agent, antibiotic resistance, Antimicrobial Cationic Peptides, Antimicrobial peptides, Bacteria (microorganisms), Bacterial adhesion, bacterial survival, Biocompatible Materials, biofilm, Biofilms, biomaterial, carbenicillin, cefazolin, Coated Materials, Biocompatible, Cytotoxicity, drug activity, drug half life, drug solubility, drug stability, drug targeting, gentamicin, human, Humans, Immobilized Proteins, Molecular Sequence Data, mouth inflammation, Nanostructured surfaces, nonhuman, penicillin G, polypeptide antibiotic agent, priority journal, review, sepsis, skin infection, soft tissue infection, Surface functionalization, Surface Properties, teicoplanin, tobramycin, vancomycin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79952187401\&partnerID=40\&md5=70d486a4c6e1ba88f804c0f0d4f4e510},
	author = {Costa, F. and Carvalho, I.F. and Montelaro, R.C. and Gomes, P. and Martins, M.C.L.}
}
@article {2233,
	title = {The effect of slurry preparation methods on biaxial flexural strength of dental porcelain},
	journal = {Journal of Prosthetic DentistryJ. Prosthet. Dent.},
	volume = {105},
	year = {2011},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JPDEAdoi: 10.1016/S0022-3913(11)60058-9PubMed ID: 21530756Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; Institute of Biomedical Engineering, Rua do Campo Alegre, 283, 4150-180 Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: Complex Mixtures; Dental Porcelain, 12001-21-7References: The Glossary of Prosthodontic Terms (2005) J Prosthet Dent, 94, pp. 10-92;(2008) Dentistry - Ceramic Materials, , ISO 6872 Geneva, Switzerland: International Organization for Standards; 
Sadowsky, S.J., An overview of treatment considerations for esthetic restorations: A review of the literature (2006) Journal of Prosthetic Dentistry, 96 (6), pp. 433-442. , DOI 10.1016/j.prosdent.2006.09.018, PII S0022391306004756; 
Messer, R.L., Lockwood, P.E., Wataha, J.C., Lewis, J.B., Norris, S., Bouillaguet, S., In vitro cytotoxicity of traditional versus contemporary dental ceramics (2003) J Prosthet Dent, 90, pp. 452-458; 
Oh, W.-S., DeLong, R., Anusavice, K.J., Factors affecting enamel and ceramic wear: A literature review (2002) Journal of Prosthetic Dentistry, 87 (4), pp. 451-459. , DOI 10.1067/mpr.2002.123851; 
Schuh, C., Kinast, E.J., Mezzomo, E., Kapczinski, M.P., Effect of glazed and polished surface finishes on the friction coefficient of two low-fusing ceramics (2005) Journal of Prosthetic Dentistry, 93 (3), pp. 245-252. , DOI 10.1016/j.prosdent.2004.12.010; 
Anusavice, K.J., Degradability of dental ceramics (1992) Adv Dent Res, 6, pp. 82-89; 
Kelly, J.R., Nishimura, I., Campbell, S.D., Ceramics in dentistry: Historical roots and current perspectives (1996) Journal of Prosthetic Dentistry, 75 (1), pp. 18-32; 
Rosenblum, M.A., Schulman, A., A Review of All-Ceramic Restorations (1997) Journal of the American Dental Association, 128 (3), pp. 297-307; 
Jung, Y.-G., Wuttiphan, S., Peterson, I.M., Lawn, B.R., Damage modes in dental layer structures (1999) Journal of Dental Research, 78 (4), pp. 887-897; 
Cesar, P.F., Yoshimura, H.N., Miranda Jr., W.G., Okada, C.Y., Correlation between fracture toughness and leucite content in dental porcelains (2005) Journal of Dentistry, 33 (9), pp. 721-729. , DOI 10.1016/j.jdent.2005.02.001, PII S0300571205000503; 
Griggs, J.A., Kishen, A., Le, K.N., Mechanism of strength increase for a hydrothermal porcelain (2003) Dent Mater, 19, pp. 625-631; 
Oilo, G., Flexural strength and internal defects of some dental porcelains (1988) Acta Odontol Scand, 46, pp. 313-322; 
Sinmazisik, G., Ove{\c c}oglu, M.L., Physical properties and microstructural characterization of dental porcelains mixed with distilled water and modeling liquid (2006) Dent Mater, 22, pp. 735-745; 
Zhang, Y., Griggs, J.A., Benham, A.W., Influence of powder/liquid mixing ratio on porosity and translucency of dental porcelains (2004) Journal of Prosthetic Dentistry, 91 (2), pp. 128-135. , DOI 10.1016/j.prosdent.2003.10.014; 
Griggs, J.A., Thompson, J.Y., Anusavice, K.J., Effects of flaw size and auto-glaze treatment on porcelain strength (1996) Journal of Dental Research, 75 (6), pp. 1414-1417; 
Dos Santos, J.G., Fonseca, R.G., Adabo, G.L., Dos Santos Cruz, C.A., Shear bond strength of metal-ceramic repair systems (2006) J Prosthet Dent, 96, pp. 165-173; 
Galiatsatos, A.A., An indirect repair technique for fractured metal-ceramic restorations: A clinical report (2005) Journal of Prosthetic Dentistry, 93 (4), pp. 321-323. , DOI 10.1016/j.prosdent.2004.12.018; 
Fleming, G.J., Shaini, F.J., Marquis, P.M., An assessment of the influence of mixing induced variability on the bi-axial flexure strength of dentine porcelain discs and the implications for laboratory testing of porcelain specimens (2000) Dent Mater, 16, pp. 114-119; 
Palin, W.M., Fleming, G.J.P., Marquis, P.M., An evaluation of the technique sensitivity of a hydrothermal low-fusing dental ceramic (2001) Journal of Dentistry, 29 (6), pp. 443-449. , DOI 10.1016/S0300-5712(01)00039-2, PII S0300571201000392; 
Zeng, K., Oden, A., Rowcliffe, D., Flexure tests on dental ceramics (1996) International Journal of Prosthodontics, 9 (5), pp. 434-439; 
Cattell, M.J., Clarke, R.L., Lynch, E.J.R., The transverse strength, reliability and microstructural features of four dental ceramics - Part I (1997) Journal of Dentistry, 25 (5), pp. 399-407. , PII S0300571296000589; 
Gill, J., Methods and results in condensation of dental porcelain (1932) J Am Dent Assoc, 19, pp. 1147-1154; 
Anusavice, K.J., Hojjatie, B., Stress distribution in metal-ceramic crowns with a facial porcelain margin (1987) J Dent Res, 66, pp. 1493-1498; 
Fleming, G.J.P., Shelton, R.M., Marquis, P.M., The influence of clinically induced variability on the bi-axial fracture strength of aluminous core porcelain discs (1999) J Dent, 27, pp. 587-594; 
Tomozawa, M., Han, W.-T., Lanford, W.A., Water entry into Silica Glass during Slow Crack Growth (1991) J Am Ceram Soc, 74, pp. 2573-2576; 
Ray, C.S., Day, D.E., Identifying internal and surface crystallization by differential thermal analysis for the glass-to-crystal transformations (1996) Thermochim Acta, 280-281, pp. 163-174; 
Mackert Jr., J.R., Williams, A.L., Ergle, J.W., Russell, C.M., Water-enhanced crystallization of leucite in dental porcelain (2000) Dent Mater, 16, pp. 426-431; 
Burgner, L.L., Weinberg, M.C., An assessment of crystal growth behavior in lithium disilicate glass (2001) J. Non-Cryst Solids, 279, pp. 28-43; 
Deubener, J., Muller, R., Behrens, H., Heide, G., Water and the glass transition temperature of silicate melts (2003) J. Non-Cryst Solids, 330, pp. 268-273; 
Seghi, R.R., Daher, T., Caputo, A., Relative flexural strength of dental restorative ceramics (1990) Dent Mater, 6, pp. 181-184; 
Quinn, J.B., Quinn, G.D., A practical and systematic review of Weibull statistics for reporting strengths of dental materials (2010) Dent Mater, 26, pp. 135-147; 
Ritter, J.E., Critique of test methods for lifetime predictions (1995) Dent Mater, 11, pp. 147-151; 
Giordano, R.A., Pelletier, L., Campbell, S., Pober, R., Flexural strength of an infused ceramic, glass ceramic, and feldspathic porcelain (1995) J Prosthet Dent, 73, pp. 411-418; 
Kelly, J.R., Perspectives on strength (1995) Dent Mater, 11, pp. 103-110; 
Griggs, J.A., Wataha, J.C., Kishen, A., Effect of hydrolyzed surface layer on the cytotoxicity and chemical resistance of a low fusing porcelain (2003) Dent Mater, 19, pp. 353-358; 
Bertolini, M.J., Zaghete, M.A., Gimenes, R., Freitas De Souza, R., Vaz, L.G., Preparation of new glass systems by the polymeric precursor method for dental applications (2004) J Non- Cryst Solids, 344, pp. 170-175; 
Tsetsekou, A., Papadopoulos, T., Adamopoulos, O., Microstructure effect on the properties of a commercial low-fusing dental porcelain (2002) Journal of Materials Science: Materials in Medicine, 13 (4), pp. 407-416. , DOI 10.1023/A:1014301021375; 
Gonzaga, C.C., Cesar, P.F., Okada, C.Y., Fredericci, C., Beneduce-Neto, F.V., Yoshimura, H.N., Mechanical properties and porosity of dental glass-ceramics hot-pressed at different temperatures (2008) Mater Res, 11, pp. 301-306; 
Pelaez, A., Garcia, C., Correa, J.C., Abad, P., Reliability Weibull analysis for structural evaluation of bioactive films obtained by sol-gel process (2004) Key Eng Mater, 254-256, pp. 431-434; 
Pham, H., (2006) Springer Handbook of Engineering Statistics, pp. 97-102. , 1st ed. London: Springer; 
Ritter, J.E., Predicting lifetimes of materials and material structures (1995) Dent Mater, 11, pp. 142-146
},
	month = {2011///},
	pages = {308 - 314},
	abstract = {Statement of problem. One-step and incremental mixing procedures are currently used to produce dental ceramic pastes. In the ceramic industry, high quality is obtained using one-step mixing, but in dentistry, the best method has not been yet determined. Purpose. The purpose of this study is to evaluate the effects of 2 mixing techniques on the biaxial flexural strength and microstructure of dental porcelain. Material and methods. Feldspathic porcelain discs (2 {\texttimes} 15 mm in diameter) were produced and divided according to the ceramic paste preparation method, powder-liquid incremental mixing group (n=50) or one-step mixing, as a control group (n=50). Specimens were tested for biaxial flexural strength and characterized using porosimetry, relative humidity, SEM/EDS, XRD, and FT-IR analyses. Statistical analysis was conducted using Weibull statistics. The Weibull modulus, characteristic strength and relative humidity were compared between groups, using Student{\textquoteright}s t-test and Mann-Whitney U test (α=.05). Results. The powder-liquid incremental mixing group showed significantly higher values (SD) of Weibull modulus (6.74 (0.70), P<.001) and characteristic strength (79.87 (2.01) MPa, P<.001) when compared to the one-step mixing group (4.94 (0.94) and 75.95 (2.61) MPa). Significantly lower mean (SD) relative humidity values (P=.009) were found for powder-liquid incremental mixing group (20\% (0.5\%)) compared to one-step mixing group (22\% (1\%)). XRD spectra showed that the one-step mixing group produced higher amounts of the amorphous phase. Conclusions. Specimens produced by the incremental mixing technique showed higher biaxial flexural strength than one-step mixing. {\textcopyright} 2011 The Editorial Council of the Journal of Prosthetic Dentistry.},
	keywords = {article, chemistry, comparative study, Complex Mixtures, Crowns, dental care, Dental Porcelain, Dental Prosthesis Design, Dental Stress Analysis, Dental Veneers, denture, drug mixture, evaluation, materials testing, pliability, tooth crown, tooth prosthesis},
	isbn = {00223913 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955604181\&partnerID=40\&md5=53c0732c254214b4a53e3222fe89f4a0},
	author = {Pelaez-Vargas, A. and Dussan, J.A. and Restrepo-Tamayo, L.F. and Paucar, C. and Ferreira, J.A. and Monteiro, F.J.}
}
@article {2220,
	title = {Friction and wear behaviour of bacterial cellulose against articular cartilage},
	journal = {WearWear},
	volume = {271},
	year = {2011},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: WEARAdoi: 10.1016/j.wear.2010.12.042Language of Original Document: EnglishCorrespondence Address: Gomes, J.R.; Department of Mechanical Engineering, University of Minho, Guimar{\~a}es, Portugal; email: jgomes@dem.uminho.ptReferences: Klemm, D., Schumann, D., Udhardt, U., Marsch, S., Bacterial synthesized cellulose-artificial blood vessels for microsurgery (2001) Prog. Polym. Sci., 26 (9), pp. 1561-1603;Vandamme, E.J., De Baets, S., Vanbaelen, A., Joris, K., De Wulf, P., Improved production of bacterial cellulose and its application potential (1998) Polym. Degrad. Stab., 59, pp. 93-99; 
Czaja, W., Krystynowicza, A., Bieleckia, S., Brown, R.M., Microbial cellulose - the natural power to heal wounds (2006) Biomaterials, 27, pp. 145-151; 
Mow, V.C., Ateshian, G.A., Lubrication and wear of diarthrodial joints (1997) Basic Orthopaedic Biomechanics, pp. 275-315. , Lippincott-Raven Publishers, Philadelphia, USA, V.C. Mow, W.C. Hayes (Eds.); 
Walker, P.S., Blunn, G.W., Biomechanical principles of total knee replacement design (1997) Basic Orthopaedic Biomechanics, pp. 461-493. , Lippincott-Raven Publishers, Philadelphia, USA, V.C. Mow, W.C. Hayes (Eds.); 
Mankin, H.J., The response of articular cartilage to mechanical injury (1982) J. Bone Joint Surg. Am., 64, pp. 460-466; 
Bray, J., Merrill, E., Poly(vinyl alcohol) hydrogels for synthetic articular cartilage materials (1973) J. Biomed. Mater. Res., 7, pp. 431-443; 
Kobayashi, M., A study of polyvinyl alcohol-hydrogel (PVA-H) artificial meniscus in vivo (2004) Bio-med. Mater. Eng., 14 (4), pp. 505-515; 
Kobayashi, M., Chang, Y.S., Oka, M., A two year in vivo study of polyvinyl alcohol hydrogel (PVA-H) artificial meniscus (2005) Biomaterials, 26 (16), pp. 3243-3248; 
Covert, R.J., Ott, R.D., Ku, D.N., Friction characteristics of a potential articular cartilage biomaterial (2003) Wear, 255, pp. 1064-1068; 
Ushio, K., Oka, M., Hyon, S.H., Hayami, T., Yura, S., Matsumura, K., Toguchida, J., Nakamura, T., Attachment of artificial cartilage to underlying bone (2004) J. Biomed. Mater. Res.: Part B - Appl. Biomater., 68 (1), pp. 59-68; 
Bodugoz-Senturk, H., Macias, C., Kung, J.H., Muratoglu, O., Poly(vinyl alcohol) - acrylamide hydrogels as load-bearing cartilage substitute (2009) Biomaterials, 30 (4), pp. 589-596; 
Bavaresco, V.P., Zavaglia, C.A.C., Reis, M.C., Gomes, J.R., Study on the tribological properties of pHEMA hydrogels for use in artificial articular cartilage (2008) Wear, 265, pp. 269-277; 
Dubois, M., Gilles, D.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for the determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356; 
Clasen, C., Sultanova, B., Wilhelms, T., Heisig, P., Kulicke, W.-M., Effects of different drying processes on the material properties of bacterial cellulose membranes (2006) Macromol. Symp., 244, pp. 48-58; 
Iguchi, M., Yamanaka, S., Budhiono, A., Bacterial cellulose - a masterpiece of nature{\textquoteright}s arts (2000) J. Mater. Sci., 35 (2), pp. 261-270; 
Wolfgang, G., Keckes, J., Tensile properties of cellulose acetate butyrate composites reinforced with bacterial cellulose (2000) Compos. Sci. Technol., 64 (15), pp. 2407-2413; 
Saarakkala, S., Laasanen, M.S., Jurvelin, J.S., Toyras, J., Quantitative ultrasound imaging detects degenerative changes in articular cartilage surface and subchondral bone (2006) Phys. Med. Biol., 51, pp. 5333-5346; 
Jurvelin, J.S., Muller, D.J., Wong, M., Studer, D., Engel, A., Hunziker, E.B., Surface and subsurface morphology of bovine humeral articular cartilage as assessed by atomic force and transmission electron microscopy (1996) J. Struct. Biol., 117, pp. 45-54; 
Crockett, R., Roos, S., Rossbach, P., Dora, C., Born, W., Troxler, H., Imaging of the surface of human and bovine articular cartilage with ESEM and AFM (2005) Tribol. Lett., 19 (4), pp. 311-317; 
Udofia, J.J., Jin, Z.M., Elastohydrodynamic lubrication analysis of metal-on-metal hip-resurfacing prostheses (2003) J. Biomech., 36, pp. 537-544; 
Pan, Y., Xiong, D., Friction properties of nano-hydroxyapatite reinforced poly(vinyl alcohol) gel composites as an articular cartilage (2009) Wear, 266, pp. 699-703; 
Merkher, Y., Sivan, S., Etsion, I., Maroudas, A., Halperin, G., Yosef, A., A rational human joint friction test using a human cartilage-on-cartilage arrangement (2006) Tribol. Lett., 22 (1), pp. 29-36; 
Zanoria, E., Danyluk, S., McNallan, M., Effects of length, diameter and population density of tribological rolls on friction between self-mated silicon (1995) Wear, 181, pp. 784-789
},
	month = {2011///},
	pages = {2328 - 2333},
	abstract = {Bacterial cellulose (BC) is a natural and biocompatible material with unique properties, such as high water holding capacity, ultra-fine fibre network and high strength that makes it an attractive material for the repair of articular cartilage lesions. However, data on the tribological properties of BC is very scarce, particularly if natural articular cartilage is involved in the contact. In this work, unmodified BC pellicles were grown from Gluconacetobacter xylinus in order to be used as tribological samples against bovine articular cartilage (BAC) in the presence of phosphate buffered saline (PBS). The tribological assessment of the sliding pairs was accomplished using reciprocating pin-on-flat tests at 37{\textdegree}C. The reciprocating sliding frequency and stroke length were kept constant at 1Hz and 8mm, respectively. Contact pressures ranging from 0.80 to 2.40MPa were applied. The friction coefficient evolution was continuously monitored during the tests and the release of total carbohydrates into the lubricating solution was followed by means of the phenol-H                        2SO
                        4 method as an attempt to evaluate wear losses. The morphology of worn surfaces was characterized by SEM/EDS and the main wear mechanisms were identified. Low friction coefficient values (\~{}0.05) combined with the preservation of the mating surfaces (BC and BAC) indicate the potential of BC to be used as artificial cartilage for articular joints. {\textcopyright} 2011 Elsevier B.V.
},
	keywords = {Articular cartilage, Articular cartilages, Articular joints, Artificial cartilages, Bacterial cellulose, Bacteriology, Biocompatible Materials, Biological materials, Biotribology, Bovine articular cartilage, Carbohydrates, Cartilage, Cellulose, Contact pressures, Fibre network, Friction, Friction and wear, Friction coefficients, Gluconacetobacter xylinus, High strength, High water, Low friction coefficients, Mating surfaces, Phenols, Phosphate-buffered salines, Pin-on-flat, Reciprocating sliding, SEM/EDS, Stroke length, Total carbohydrates, Tribological properties, Tribology, Wear, Wear loss, Wear mechanisms, Worn surface},
	isbn = {00431648 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960657605\&partnerID=40\&md5=70f685f10e794e0146dfcea106d860e3},
	author = {Lopes, J.L. and Machado, J.M. and Castanheira, L. and Granja, P.L. and Gama, F.M. and Dourado, F. and Gomes, J.R.}
}
@article {2232,
	title = {Interactions of leukocytes and platelets with poly(lysine/leucine) immobilized on tetraethylene glycol-terminated self-assembled monolayers},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2011.01.013PubMed ID: 21237292Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; leucine, 61-90-5, 7005-03-0; polylysine, 25104-18-1, 25988-63-0, 33960-24-6, 38000-06-5, 73565-56-7; Ethylene Glycol, 107-21-1; Imidazoles; Immobilized Proteins; N,N-carbonyldiimidazole, 530-62-1; Polyethylene Glycols; Polylysine, 25104-18-1; ProteinsReferences: Dailey, J., (1998) Blood, , 2nd ed. Ipswich (MA, USA): Medical Consulting Group;Section V: Chronic intermittent haemodialysis and prevention of clotting in the extracorporal system (2002) Nephrol Dial Transplant, 17 (SUPPL. 7), pp. 63-71. , European Best Practice Guidelines Working Group; 
Martins, M.C., Curtin, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res A, 88, pp. 162-173; 
Love, J.C., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chemical Reviews, 105 (4), pp. 1103-1169. , DOI 10.1021/cr0300789; 
Cardin, A.D., Weintraub, H.J.R., Molecular modeling of protein-glycosaminoglycan interactions (1989) Arteriosclerosis, 9 (1), pp. 21-32; 
Prime, K.L., Whitesides, G.M., Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide) - A model system using self-assembled monolayers (1993) J Am Chem Soc, 115, pp. 10714-10721; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) Journal of Biomedical Materials Research - Part A, 67 (1), pp. 158-171; 
Werner, C., Konig, U., Augsburg, A., Arnhold, C., Korber, H., Zimmermann, R., Jacobasch, H.-J., Electrokinetic surface characterization of biomedical polymers - A survey (1999) Colloids and Surfaces A: Physicochemical and Engineering Aspects, 159 (2-3), pp. 519-529. , DOI 10.1016/S0927-7757(99)00290-3, PII S0927775799002903; 
Frank, R.D., Dresbach, H., Thelen, H., Sieberth, H.-G., Glutardialdehyde induced fluorescence technique (GIFT): A new method for the imaging of platelet adhesion on biomaterials (2000) Journal of Biomedical Materials Research, 52 (2), pp. 374-381. , DOI 10.1002/1097-4636(200011)52:2<374::AID-JBM18>3.0.CO;2-Z; 
Chan, Y.H.M., Schweiss, R., Werner, C., Grunze, M., Electrokinetic characterization of oligo- and poly(ethylene glycol)-terminated self-assembled monolayers on gold and glass surfaces (2003) Langmuir, 19, pp. 7380-7385; 
Schweiss, R., Welzel, P.B., Werner, C., Knoll, W., Dissociation of surface functional groups and preferential adsorption of ions on self-assembled monolayers assessed by streaming potential and streaming current measurements (2001) Langmuir, 17 (14), pp. 4304-4311. , DOI 10.1021/la001741g; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27 (31), pp. 5357-5367. , DOI 10.1016/j.biomaterials.2006.06.010, PII S0142961206005515; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res, 89 A, pp. 642-653; 
Prime, K.L., Whitesides, G.M., Self-assembled organic monolayers: Model systems for studying adsorption of proteins at surfaces (1991) Science, 252 (5009), pp. 1164-1167; 
Mrksich, M., Using self-assembled monolayers to model the extracellular matrix (2009) Acta Biomater, 5, pp. 832-841; 
Sagnella, S., Mai-Ngam, K., Chitosan based surfactant polymers designed to improve blood compatibility on biomaterials (2005) Colloids Surf B Biointerfaces, 42, pp. 147-155; 
Chuang, W.H., Lin, J.C., Surface characterization and platelet adhesion studies for the mixed self-assembled monolayers with amine and carboxylic acid terminated functionalities (2007) J Biomed Mater Res A, 82, pp. 820-830; 
Chen, S., Yu, F., Yu, Q., He, Y., Jiang, S., Strong resistance of a thin crystalline layer of balanced charged groups to protein adsorption (2006) Langmuir, 22 (19), pp. 8186-8191. , DOI 10.1021/la061012m; 
Chen, S.F., Jiang, S.Y., A new avenue to nonfouling materials (2008) Adv Mater, 20, p. 335; 
Li, D., Chen, H., Glenn McClung, W., Brash, J.L., Lysine-PEG-modified polyurethane as a fibrinolytic surface. effect of PEG chain length on protein interactions, platelet interactions and clot lysis (2009) Acta Biomater, 5, pp. 1864-1871; 
Tsai, W.-B., Grunkemeier, J.M., Horbett, T.A., Variations in the ability of adsorbed fibrinogen to mediate platelet adhesion to polystyrene-based materials: A multivariate statistical analysis of antibody binding to the platelet binding sites of fibrinogen (2003) Journal of Biomedical Materials Research - Part A, 67 (4), pp. 1255-1268; 
Weisel, J.W., Nagaswami, C., Vilaire, G., Bennett, J.S., Examination of the platelet membrane glycoprotein IIb-IIIa complex and its interaction with fibrinogen and other ligands by electron microscopy (1992) J Biol Chem, 267 (23), pp. 16637-16643; 
Gorbet, M.B., Sefton, M.V., Biomaterial-associated thrombosis: Roles of coagulation factors, complement, platelets and leukocytes (2004) Biomaterials, 25 (26), pp. 5681-5703. , DOI 10.1016/j.biomaterials.2004.01.023, PII S0142961204000687; 
Brash, J.L., Tenhove, P., Protein adsorption studies on standard polymeric materials (1993) J Biomater Sci Polym Ed, 4, pp. 591-599; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) Journal of the American Chemical Society, 120 (14), pp. 3464-3473. , DOI 10.1021/ja970819l; 
Silin, V., Weetall, H., Vanderah, D.J., SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs) (1997) Journal of Colloid and Interface Science, 185 (1), pp. 94-103. , DOI 10.1006/jcis.1996.4586; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (2000) Journal of Biomedical Materials Research, 49 (4), pp. 435-447. , DOI 10.1002/(SICI)1097-4636(20000315)49:4<435::AID-JBM2>3.0.CO;2-Y; 
Werthen, M., Sellborn, A., Kalltorp, M., Elwing, H., Thomsen, P., In vitro study of monocyte viability during the initial adhesion to albumin- and fibrinogen-coated surfaces (2001) Biomaterials, 22, pp. 827-832; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) Journal of Biomedical Materials Research, 50 (3), pp. 291-301. , DOI 10.1002/(SICI)1097-4636(20000605)50:3<291::AID-JBM2>3.0.CO;2-1; 
Barbosa, J.N., Martins, M.C.L., Freitas, S.C., Goncalves, I.C., Aguas, A.P., Barbosa, M.A., Adhesion of human leucocytes on mixtures of hydroxyl- and methyl-terminated self-assembled monolayers: Effect of blood protein adsorption (2010) J Biomed Mater Res, 93 A, pp. 12-19; 
Sperling, C., Schweiss, R.B., Streller, U., Werner, C., In vitro hemocompatibility of self-assembled monolayers displaying various functional groups (2005) Biomaterials, 26, pp. 6547-6557; 
Toda, M., Kitazawa, T., Hirata, I., Hirano, Y., Iwata, H., Complement activation on surfaces carrying amino groups (2008) Biomaterials, 29 (4), pp. 407-417. , DOI 10.1016/j.biomaterials.2007.10.005, PII S014296120700796X
},
	month = {2011///},
	pages = {1949 - 1955},
	abstract = {Surfaces that bind heparin are important for biomaterials for blood deheparinization. In our recent work it was demonstrated that a polypeptide composed of l-lysine and l-leucine (pKL), after immobilization onto tetra(ethylene glycol) terminated self-assembled monolayers (EG4-SAMs), can bind heparin from blood plasma in a selective, concentration-dependent way. During this work the effect of this peptide on platelet adhesion and activation and leukocyte adhesion was studied. The surface charge of these nanostructured surfaces was evaluated in order to correlate the effect of positively charged amine groups and hydrophobic methyl groups on the behavior of platelets and leukocyte adhesion. The results demonstrated that the presence of pKL decreased leukocyte adhesion to EG4-SAMs at all concentrations used. This effect is even more pronounced when surfaces were pre-immersed in heparinized plasma. In contrast, there is an increase in platelet adhesion and activation with increased percentage immobilized pKL. This effect is enhanced when surfaces were pre-immersed in heparinized plasma. However, adsorbed pKL in very low amounts does not induce platelet adhesion and activation compared with EG4, even when pre-immersed in plasma. Since only low pKL amounts are necessary to induce heparin selectivity, these results are promising for the development of heparin-binding biomaterials for blood deheparinization. {\textcopyright} 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {article, Blood Platelets, Cell Communication, Cell Shape, Ethylene Glycol, heparin, heparin binding protein, Humans, hydrophobicity, Imidazoles, Immobilized Proteins, leucine, leukocyte, leukocyte adherence, Leukocytes, methyl group, nanomaterial, plasma, Platelet Adhesiveness, Platelets, Polyethylene Glycols, polylysine, polypeptide, priority journal, Proteins, self assembled monolayer, Self-assembled monolayers, static electricity, Surface charge, Surface Properties, Tetra, thrombocyte, thrombocyte adhesion},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953868334\&partnerID=40\&md5=2cb5b44223fe6e8106b513b80689103d},
	author = {Martins, M.C.L. and Ochoa-Mendes, V. and Ferreira, G. and Barbosa, J.N. and Curtin, S.A. and Ratner, B.D. and Barbosa, M.A.}
}
@article {2228,
	title = {Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants},
	journal = {Dental MaterialsDent. Mater.},
	volume = {27},
	year = {2011},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: DEMAEdoi: 10.1016/j.dental.2011.02.014Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: resazurin, 550-82-3; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; yttrium, 7440-65-5; zirconium, 14940-68-2, 7440-67-7; Ceramics; Coated Materials, Biocompatible; Dental Implants; Dental Materials; Indicators and Reagents; Oxazines; Silicon Dioxide, 7631-86-9; Xanthenes; Yttrium, 7440-65-5; Zirconium, 7440-67-7; resazurin, 550-82-3; yttria stabilized tetragonal zirconiaReferences: Kasemo, B., Biocompatibility of titanium implants: Surface science aspects (1983) J Prosthet Dent, 49, pp. 832-837;Egusa, H., Ko, N., Shimazu, T., Yatani, H., Suspected association of an allergic reaction with titanium dental implants: A clinical report (2008) J Prosthet Dent, 100, pp. 344-347; 
Tschernitschek, H., Borchers, L., Geurtsen, W., Nonalloyed titanium as a bioinert metal - A review (2005) Quintessence International, 36 (7), pp. 523-530; 
Oliva, J., Oliva, X., Oliva, J., One-year follow-up of first consecutive 100 zirconia dental implants in human: A comparison of 2 different rough surfaces (2007) Int J Oral Maxillofac Implants, 22, pp. 430-435; 
Johansson, C.B., Hansson, H.A., Albrektsson, T., Qualitative interfacial study between bone and tantalum, niobium or commercially pure titanium (1990) Biomaterials, 11, pp. 277-280; 
De Wijs, F.L., Van Dongen, R.C., De Lange, G.L., De Putter, C., Front tooth replacement with Tubingen (Frialit) implants (1994) J Oral Rehabil, 21, pp. 11-26; 
Albrektsson, T., Lekholm, U., Osseointegration: Current state of the art (1989) Dent Clin North Am, 33, pp. 537-554; 
Albrektsson, T., Sennerby, L., State of the art in oral implants (1991) J Clin Periodontol, 18, pp. 474-481; 
Albrektsson, T., Sennerby, L., Wennerberg, A., State of the art of oral implants (2008) Periodontol 2000, 47, pp. 15-26; 
Sennerby, L., Dental implants: Matters of course and controversies (2008) Periodontol 2000, 47, pp. 9-14; 
Leblebicioglu, B., Rawal, S., Mariotti, A., A review of the functional and esthetic requirements for dental implants (2007) J Am Dent Assoc, 138, pp. 321-329; 
Chevalier, J., What future for zirconia as a biomaterial? (2006) Biomaterials, 27 (4), pp. 535-543. , DOI 10.1016/j.biomaterials.2005.07.034, PII S0142961205007039; 
Hochman, N., Zalkind, M., New all-ceramic indirect post-and-core system (1999) J Prosthet Dent, 81, pp. 625-629; 
Malmstrom, J., Slotte, C., Adolfsson, E., Norderyd, O., Thomsen, P., Bone response to free form-fabricated hydroxyapatite and zirconia scaffolds: A histological study in the human maxilla (2009) Clin Oral Implants Res, 20, pp. 379-385; 
Manicone, P.F., Rossi Iommetti, P., Raffaelli, L., An overview of zirconia ceramics: Basic properties and clinical applications (2007) J Dent, 35, pp. 819-826; 
Kim, H.-W., Kim, H.-E., Salih, V., Knowles, J.C., Dissolution control and cellular responses of calcium phosphate coatings on zirconia porous scaffold (2004) Journal of Biomedical Materials Research - Part A, 68 (3), pp. 522-530; 
Kim, H.W., Shin, S.Y., Kim, H.E., Lee, Y.M., Chung, C.P., Lee, H.H., Bone formation on the apatite-coated zirconia porous scaffolds within a rabbit calvarial defect (2008) J Biomater Appl, 22, pp. 485-504; 
Akagawa, Y., Hosokawa, R., Sato, Y., Kamayama, K., Comparison between freestanding and tooth-connected partially stabilized zirconia implants after two years{\textquoteright} function in monkeys: A clinical and histologic study (1998) J Prosthet Dent, 80, pp. 551-558; 
Kohal, R.J., Att, W., Bachle, M., Butz, F., Ceramic abutments and ceramic oral implants. An update (2008) Periodontol 2000, 47, pp. 224-243; 
Degidi, M., Artese, L., Scarano, A., Perrotti, V., Gehrke, P., Piattelli, A., Inflammatory infiltrate, microvessel density, nitric oxide synthase expression, vascular endothelial growth factor expression, and proliferative activity in peri-implant soft tissues around titanium and zirconium oxide healing caps (2006) Journal of Periodontology, 77 (1), pp. 73-80. , DOI 10.1902/jop.2006.77.1.73; 
Kohal, R.J., Klaus, G., A zirconia implant-crown system: A case report (2004) International Journal of Periodontics and Restorative Dentistry, 24 (2), pp. 147-153; 
Sennerby, L., Dasmah, A., Larsson, B., Iverhed, M., Bone tissue responses to surface-modified zirconia implants: A histomorphometric and removal torque study in the rabbit (2005) Clin Implant Dent Relat Res, 71, pp. 13-20; 
Covacci, V., Bruzzese, N., Maccauro, G., Andreassi, C., Ricci, G.A., Piconi, C., Marmo, E., Cittadini, A., In vitro evaluation of the mutagenic and carcinogenic power of high purity zirconia ceramic (1999) Biomaterials, 20 (4), pp. 371-376. , DOI 10.1016/S0142-9612(98)00182-3, PII S0142961298001823; 
Amaral, M., Costa, M.A., Lopes, M.A., Silva, R.F., Santos, J.D., Fernandes, M.H., Si 3N 4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells (2002) Biomaterials, 23 (24), pp. 4897-4906. , DOI 10.1016/S0142-9612(02)00249-1, PII S0142961202002491; 
Garvie, R.C., Hannink, R.H., Pascoe, R.T., Ceramic steel? (1975) Nature, 258, pp. 703-704; 
Denry, I., Kelly, J.R., State of the art of zirconia for dental applications (2008) Dent Mater, 24, pp. 299-307; 
Ghosh, A., Suri, A.K., Pandey, M., Thomas, S., Rama Mohan, T.R., Rao, B.T., Nanocrystalline zirconia-yttria system-a Raman study (2006) Materials Letters, 60 (9-10), pp. 1170-1173. , DOI 10.1016/j.matlet.2005.10.102, PII S0167577X05010888; 
Li, M., Feng, Z., Xiong, G., Ying, P., Xin, Q., Li, C., Phase transformation in the surface region of zirconia detected by UV Raman spectroscopy (2001) J Phys Chem B, 105, pp. 8107-8111; 
Lughi, V., Clarke, D.R., Temperature dependence of the yttria-stabilized zirconia Raman spectrum (2007) J Appl Phys, 101; 
Argyriou, D.N., Howard, C.J., Re-investigation of yttria-tetragonal zirconia polycrystal (Y-TZP) by neutron powder diffraction - A cautionary tale (1995) J Appl Crystallogr, 28, pp. 206-208; 
Pascual, C., Duran, P., Subsolidus phase equilibria and ordering in the system ZrO 2-Y 2O 3 (1983) J Am Ceram Soc, 66, p. 23; 
Kelly, J.R., Denry, I., Stabilized zirconia as a structural ceramic: An overview (2008) Dent Mater, 24, pp. 289-298; 
Lughi, V., Sergo, V., Low temperature degradation aging of zirconia: A critical review of the relevant aspects in dentistry (2010) Dent Mater, 26, pp. 807-820; 
Chevalier, J., De Aza, A.H., Fantozzi, G., Schehl, M., Torrecillas, R., Extending the lifetime of ceramic orthopaedic implants (2000) Advanced Materials, 12 (21), pp. 1619-1621. , DOI 10.1002/1521-4095(200011)12:21<1619::AID-ADMA1619>3.0.CO;2-O; 
Kim, H.W., Kong, Y.M., Bae, C.J., Noh, Y.J., Kim, H.E., Sol-gel derived fluoro-hydroxyapatite biocoatings on zirconia substrate (2004) Biomaterials, 25, pp. 2919-2926; 
Lee, E.-J., Lee, S.-H., Kim, H.-W., Kong, Y.-M., Kim, H.-E., Fluoridated apatite coatings on titanium obtained by electron-beam deposition (2005) Biomaterials, 26 (18), pp. 3843-3851. , DOI 10.1016/j.biomaterials.2004.10.019, PII S0142961204009391; 
Vorobyev, A.Y., Guo, C., Femtosecond laser structuring of titanium implants (2007) Applied Surface Science, 253 (17), pp. 7272-7280. , DOI 10.1016/j.apsusc.2007.03.006, PII S0169433207004400; 
Perrie, W., Rushton, A., Gill, M., Fox, P., O{\textquoteright}Neill, W., Femtosecond laser micro-structuring of alumina ceramic (2005) Applied Surface Science, 248 (1-4), pp. 213-217. , DOI 10.1016/j.apsusc.2005.03.005, PII S0169433205003806, 4th International Conference on Photo-Excited Processes and Applications 4-ICEPA; 
Teixeira, S., Monteiro, F.J., Ferraz, M.P., Vilar, R., Eugenio, S., Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies (2007) Journal of Biomedical Materials Research - Part A, 81 (4), pp. 920-929. , DOI 10.1002/jbm.a.31073; 
Nayab, S.N., Jones, F.H., Olsen, I., Effects of calcium ion implantation on human bone cell interaction with titanium (2005) Biomaterials, 26 (23), pp. 4717-4727. , DOI 10.1016/j.biomaterials.2004.11.044, PII S0142961204010488; 
Kosmac, T., Oblak, C., Jevnikar, P., Funduk, N., Marion, L., The effect of surface grinding and sandblasting on flexural strength and reliability of Y-TZP zirconia ceramic (1999) Dental Materials, 15 (6), pp. 426-433. , PII S0109564199000706; 
Filiaggi, M.J., Pilliar, R.M., Abdulla, D., Evaluating sol-gel ceramic thin films for metal implant applications. II. Adhesion and fatigue properties of zirconia films on Ti-6Al-4V (1996) Journal of Biomedical Materials Research, 33 (4), pp. 239-256. , DOI 10.1002/(SICI)1097-4636(199624)33:4<239::AID-JBM4>3.0.CO;2-Q; 
Sollazzo, V., Pezzetti, F., Scarano, A., Piattelli, A., Bignozzi, C.A., Massari, L., Zirconium oxide coating improves implant osseointegration in vivo (2008) Dent Mater, 24, pp. 357-361; 
Sollazzo, V., Palmieri, A., Pezzetti, F., Bignozzi, C.A., Argazzi, R., Massari, L., Genetic effect of zirconium oxide coating on osteoblast-like cells (2008) J Biomed Mater Res B: Appl Biomater, 84, pp. 550-558; 
Kim, H.-W., Georgiou, G., Knowles, J.C., Koh, Y.-H., Kim, H.-E., Calcium phosphates and glass composite coatings on zirconia for enhanced biocompatibility (2004) Biomaterials, 25 (18), pp. 4203-4213. , DOI 10.1016/j.biomaterials.2003.10.094, PII S0142961203010470; 
Thian, E.S., Huang, J., Best, S.M., Barber, Z.H., Brooks, R.A., Rushton, N., Bonfield, W., The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films (2006) Biomaterials, 27 (13), pp. 2692-2698. , DOI 10.1016/j.biomaterials.2005.12.019, PII S0142961205011816; 
Klein, L., Sol-gel processing of silicates (1985) Annu Rev Mater Sci, 15, pp. 227-248; 
McDonagh, C., Sheridan, F., Butler, T., MacCraith, B.D., Characterisation of sol-gel-derived silica films (1996) Journal of Non-Crystalline Solids, 194 (1-2), pp. 72-77; 
Hench, L.L., Sol-gel materials for bioceramic applications (1997) Current Opinion in Solid State and Materials Science, 2 (5), pp. 604-610. , PII S1359028697800538; 
Garcia, C., Cere, S., Duran, A., Bioactive coatings prepared by sol-gel on stainless steel 316L (2004) J Non-Cryst Solids, 348, pp. 218-224; 
Garcia, C., Galliano, P., Cere, S., Electrochemical evaluation of resistance to localised corrosion of vitreous coatings containing particles applied on metallic substrates for biomedical applications (2003) Mater Lett, 57, pp. 1810-1814; 
Xia, Y., Whitesides, G.M., Soft lithography (1998) Angew Chem Int Ed, 37, pp. 550-575; 
Kim, E.J., Boehm, C.A., Mata, A., Fleischman, A.J., Muschler, G.F., Roy, S., Post microtextures accelerate cell proliferation and osteogenesis (2010) Acta Biomater, 6, pp. 160-169; 
Turner, A.M.P., Dowell, N., Turner, S.W.P., Kam, L., Isaacson, M., Turner, J.N., Craighead, H.G., Shain, W., Attachment of astroglial cells to microfabricated pillar arrays of different geometries (2000) Journal of Biomedical Materials Research, 51 (3), pp. 430-441. , DOI 10.1002/1097-4636(20000905)51:3<430::AID-JBM18>3.0.CO;2-C; 
Brunette, D.M., In vitro models of biological responses to implants (1999) Adv Dent Res, 13, p. 35; 
Chen, C.S., Mrksich, M., Huang, S., Whitesides, G.M., Ingber, D.E., Geometric control of cell life and death (1997) Science, 276 (5317), pp. 1425-1428. , DOI 10.1126/science.276.5317.1425; 
Flemming, R.G., Murphy, C.J., Abrams, G.A., Goodman, S.L., Nealey, P.F., Effects of synthetic micro- and nano-structured surfaces on cell behavior (1999) Biomaterials, 20 (6), pp. 573-588. , DOI 10.1016/S0142-9612(98)00209-9, PII S0142961298002099; 
Walboomers, X.F., Jansen, J.A., Cell and tissue behavior on micro-grooved surfaces (2001) Odontology, 89, pp. 2-11; 
Wilkinson, C.D.W., Riehle, M., Wood, M., Gallagher, J., Curtis, A.S.G., The use of materials patterned on a nano- and micro-metric scale in cellular engineering (2002) Materials Science and Engineering C, 19 (1-2), pp. 263-269. , DOI 10.1016/S0928-4931(01)00396-4, PII S0928493101003964, Current trends in Nanotechnologies: From Materials to Systems; 
Bettinger, C.J., Langer, R., Borenstein, J.T., Engineering substrate topography at the micro- and nanoscale to control cell function (2009) Angew Chem Int Ed, 48, pp. 5406-5415; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Cells spreading on micro-fabricated silica thin film coatings (2009) Microsc Microanal, 15, pp. 77-78; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D.J., Monteiro, F.J., Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process (2009) Key Eng Mater, 396-398, p. 303; 
Singhvi, R., Stephanopoulos, G., Wang, D.I., Effects of substratum morphology on cell physiology (1994) Biotechnol Bioeng, 43, pp. 764-771; 
Von Recum, A.F., Van Kooten, T.G., The influence of micro-topography on cellular response and the implications for silicone implants (1995) J Biomater Sci Polym Ed, 7, pp. 181-198; 
Pelaez-Vargas, A., Gallego-Perez, D., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping (2010) Microsc Microanal, 16, pp. 670-676; 
Brett, P.M., Harle, J., Salih, V., Mihoc, R., Olsen, I., Jones, F.H., Tonetti, M., Roughness response genes in osteoblasts (2004) Bone, 35 (1), pp. 124-133. , DOI 10.1016/j.bone.2004.03.009, PII S8756328204001140; 
Klinge, B., Hultin, M., Berglundh, T., Peri-implantitis (2005) Dental Clinics of North America, 49 (3 SPEC. ISS.), pp. 661-676. , DOI 10.1016/j.cden.2005.03.007, PII S0011853205000236; 
Leonhardt, A., Renvert, S., Dahlen, G., Microbial findings at failing implants (1999) Clin Oral Implants Res, 10, pp. 339-345; 
Zinelis, S., Thomas, A., Syres, K., Silikas, N., Eliades, G., Surface characterization of zirconia dental implants (2010) Dent Mater, 26, pp. 295-305; 
Marzolin, C., Smith, S.P., Prentiss, M., Whitesides, G.M., Fabrication of glass microstructures by micro-molding of sol-gel precursors (1998) Advanced Materials, 10 (8), pp. 571-574; 
Santucci, S., Di Nardo, S., Lozzi, L., Passacantando, M., Picozzi, P., XPS analysis on SiO 2 sol-gel thin films (1995) J Electron Spectrosc Relat Phenom, 76, pp. 623-628; 
Braet, F., De Zanger, R., Wisse, E., Drying cells for SEM, AFM and TEM by hexamethyldisilazane: A study on hepatic endothelial cells (1997) Journal of Microscopy, 186 (1), pp. 84-87
},
	month = {2011///},
	pages = {581 - 589},
	abstract = {Titanium implants are the gold standard in dentistry; however, problems such as gingival tarnishing and peri-implantitis have been reported. For zirconia to become a competitive alternative dental implant material, surface modification techniques that induce guided tissue growth must be developed. Objectives: To develop alternative surface modification techniques to promote guided tissue regeneration on zirconia materials, for applications in dental implantology. Methods: A methodology that combined soft lithography and sol-gel chemistry was used to obtain isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates. The materials were characterized via chemical, structural, surface morphology approaches. In vitro biological behavior was evaluated in terms of early adhesion and viability/metabolic activity of human osteoblast-like cells. Statistical analysis was conducted using one-way ANOVA/Tukey HSD post hoc test. Results: Isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates were obtained using a combined approach based on sol-gel technology and soft lithography. Micropatterned silica surfaces exhibited a biocompatible behavior, and modulated cell responses (i.e. inducing early alignment of osteoblast-like cells). After 7 d of culture, the cells fully covered the top surfaces of pillar microstructured silica films. Significance: The micropatterned silica films on zirconia showed a biocompatible response, and were capable of inducing guided osteoblastic cell adhesion, spreading and propagation. The results herein presented suggest that surface-modified ceramic implants via soft lithography and sol-gel chemistry could potentially be used to guide periodontal tissue regeneration, thus promoting tight tissue apposition, and avoiding gingival retraction and peri-implantitis. {\textcopyright} 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Adhesion, article, Biological behavior, biomaterial, biomedical and dental materials, cell adhesion, Cell culture, Cell Line, cell proliferation, Cell response, cell survival, Ceramic implants, ceramics, chemistry, Coated Materials, Biocompatible, Coatings, confocal microscopy, crystallography, Dental implant materials, Dental implantology, Dental Implants, Dental Materials, Dental prostheses, diagnostic agent, dyes, reagents, indicators, markers and buffers, Gels, Gold standards, Growth kinetics, Guided tissue regeneration, Guided Tissue Regeneration, Periodontal, heat, Hot Temperature, human, Human osteoblast-like cells, Humans, In-vitro, Indicators and Reagents, Lithography, materials testing, Micropatterned, Microscopy, Confocal, Microscopy, Electron, Scanning, osteoblast, Osteoblast-like cells, Osteoblastic cell adhesion, Osteoblasts, oxazine derivative, Oxazines, Peri-implantitis, Periodontal tissue, periodontics, phase transition, physiology, Raman spectrometry, resazurin, Scanning electron microscopy, Silica, Silica coatings, Silica film, Silica surface, silicon dioxide, Soft lithography, Sol-gel, Sol-gel chemistry, Sol-gel process, Sol-gel technology, Sol-gels, Sols, Spectrum Analysis, Raman, Stabilized zirconia, Statistical analysis, Surface modification techniques, Surface morphology, Surface Properties, surface property, Surface-modified, Surfaces, time, Time Factors, Tissue, Tissue growth, Titanium, Titanium implants, tooth implantation, Top surface, X ray diffraction, X-Ray Diffraction, xanthene derivative, Xanthenes, yttria stabilized tetragonal zirconia, Yttria stabilized zirconia, yttrium, Yttrium alloys, Zirconia, Zirconia materials, zirconium},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955638770\&partnerID=40\&md5=31e0130d40aa381f08a781115b43fe2e},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Magallanes-Perdomo, M. and Fernandes, M.H. and Hansford, D.J. and De Aza, A.H. and Pena, P. and Monteiro, F.J.}
}
@article {2215,
	title = {Layer-by-layer self-assembly of chitosan and poly(γ-glutamic acid) into polyelectrolyte complexes},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {12},
	year = {2011},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm2008235PubMed ID: 22032302Language of Original Document: EnglishCorrespondence Address: Antunes, J.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Porto, Portugal; email: joana.antunes@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; Biocompatible Materials; Chitosan, 9012-76-4; Electrolytes; Polyglutamic Acid, 25513-46-6; Viscoelastic Substances; poly(gamma-glutamic acid)References: Bertrand, P., Jonas, A., Laschewsky, A., Legras, R., Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: Suitable materials, structure and properties (2000) Macromol. Rapid Commun., 21, pp. 319-348;Schonhoff, M., Layered polyelectrolyte complexes: Physics of formation and molecular properties (2003) J. Phys.: Condens. Matter, 15, pp. R1781-R1808; 
Von Klitzing, R., Internal structure of polyelectrolyte multilayer assemblies (2006) Phys. Chem. Chem. Phys., 8, pp. 5012-5033; 
Iler, R.K., Multilayers of colloidal particles (1966) J. Colloid Interface Sci., 21, pp. 569-594; 
Zhang, X., Chen, H., Zhang, H., Layer-by-layer assembly: From conventional to unconventional methods (2007) Chemical Communications, (14), pp. 1395-1405. , DOI 10.1039/b615590a; 
Decher, G., Hong, J.D., Schmitt, J., Buildup of ultrathin multilayer films by a self-assembly process. 3. Consecutively alternating adsorption of anionic and cationic polyelectrolytes on charged surfaces (1992) Thin Solid Films, 210, pp. 831-835; 
Boudou, T., Crouzier, T., Ren, K.F., Blin, G., Picart, C., Multiple functionalities of polyelectrolyte multilayer films: New biomedical applications (2010) Adv. Mater., 22, pp. 441-467; 
Schlenoff, J.B., Retrospective on the future of polyelectrolyte multilayers (2009) Langmuir, 25, pp. 14007-14010; 
Ariga, K., Hill, J.P., Ji, Q.M., Layer-by-layer assembly as a versatile bottom-up nanofabrication technique for exploratory research and realistic application (2007) Phys. Chem. Chem. Phys., 9, pp. 2319-2340; 
Meng, S., Liu, Z., Shen, L., Guo, Z., Chou, L.L., Zhong, W., Du, Q., Ge, J., The effect of a layer-by-layer chitosan-heparin coating on the endothelialization and coagulation properties of a coronary stent system (2009) Biomaterials, 30, pp. 2276-2283; 
De Geest, B.G., Jonas, A.M., Demeester, J., De Smedt, S.C., Glucose-responsive polyelectrolyte capsules (2006) Langmuir, 22 (11), pp. 5070-5074. , DOI 10.1021/la053368o; 
Dimitrova, M., Affolter, C., Meyer, F., Nguyen, I., Richard, D.G., Schuster, C., Bartenschlager, R., Baumert, T.F., Sustained delivery of siRNAs targeting viral infection by celldegradable multilayered polyelectrolyte films (2008) Proc. Natl. Acad. Sci. U.S.A., 105, pp. 16320-16325; 
Crouzier, T., Ren, K., Nicolas, C., Roy, C., Picart, C., Layer-bylayer films as a biomimetic reservoir for rhBMP-2 delivery: Controlled differentiation of myoblasts to osteoblasts (2009) Small, 5, pp. 598-608; 
He, L., Dexter, A.F., Middelberg, A.P.J., Biomolecular engineering at interfaces (2006) Chemical Engineering Science, 61 (3), pp. 989-1003. , DOI 10.1016/j.ces.2005.05.064, PII S000925090500518X, Biomolecular Engineering; 
Chua, P.H., Neoh, K.G., Shi, Z., Kang, E.T., Structural stability and bioapplicability assessment of hyaluronic acid-chitosan polyelectrolyte multilayers on titanium substrates (2008) J. Biomed. Mater. Res., Part A, 87 A, pp. 1061-1074; 
Decher, G., Fuzzy nanoassemblies: Toward layered polymeric multicomposites (1997) Science, 277 (5330), pp. 1232-1237. , DOI 10.1126/science.277.5330.1232; 
Ozin, G.A., Hou, K., Lotsch, B.V., Cademartiri, L., Puzzo, D.P., Scotognella, F., Ghadimi, A., Thomson, J., Nanofabrication by selfassembly (2009) Mater. Today, 12, pp. 12-23; 
Dubas, S.T., Schlenoff, J.B., Factors controlling the growth of polyelectrolyte multilayers (1999) Macromolecules, 32 (24), pp. 8153-8160. , DOI 10.1021/ma981927a; 
Kumar, M.N.V.R., Muzzarelli, R.A.A., Muzzarelli, C., Sashiwa, H., Domb, A.J., Chitosan chemistry and pharmaceutical perspectives (2004) Chemical Reviews, 104 (12), pp. 6017-6084. , DOI 10.1021/cr030441b; 
Yi, H., Wu, L.-Q., Bentley, W.E., Ghodssi, R., Rubloff, G.W., Culver, J.N., Payne, G.F., Biofabrication with chitosan (2005) Biomacromolecules, 6 (6), pp. 2881-2894. , DOI 10.1021/bm050410l; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57 (1), pp. 35-52. , DOI 10.1016/S0939-6411(03)00160-7; 
Rinaudo, M., Chitin and chitosan: Properties and applications (2006) Progress in Polymer Science (Oxford), 31 (7), pp. 603-632. , DOI 10.1016/j.progpolymsci.2006.06.001, PII S0079670006000530; 
Sogias, I.A., Khutoryanskiy, V.V., Williams, A.C., Exploring the factors affecting the solubility of chitosan in water (2010) Macromol. Chem. Phys., 211, pp. 426-433; 
Sorlier, P., Denuziere, A., Viton, C., Domard, A., Relation between the degree of acetylation and the electrostatic properties of chitin and chitosan (2001) Biomacromolecules, 2 (3), pp. 765-772. , DOI 10.1021/bm015531+; 
Krayukhina, M.A., Samoilova, N.A., Yamskov, I.A., Polyelectrolyte complexes of chitosan: Formation, properties, and applications (2008) Usp. Khim., 77, pp. 854-869; 
Buescher, J.M., Margaritis, A., Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries (2007) Critical Reviews in Biotechnology, 27 (1), pp. 1-19. , DOI 10.1080/07388550601166458, PII 773236840; 
Shih, I.L., Van, Y.T., Shen, M.H., Biomedical applications of chemically and microbiologically synthesized poly(glutamic acid) and poly(lysine) (2004) Mini-Rev. Med. Chem., 4, pp. 179-188; 
Nelson, D.L., Cox, M.M., Amino acids peptides and proteins (2005) Lehninger Principles of Biochemistry, pp. 78-79. , 4th ed.; W. H. Freeman and Company: New York; 
Edde, B., Rossier, J., Lecaer, J.P., Desbruyeres, E., Gros, F., Denoulet, P., Posttranslational glutamylation of alpha-tubulin (1990) Science, 247, pp. 83-85; 
Candela, T., Fouet, A., Poly-γ-glutamate in bacteria (2006) Mol. Microbiol., 60, pp. 1091-1098; 
Rhie, G.-E., Roehrl, M.H., Mourez, M., Collier, R.J., Mekalanos, J.J., Wang, J.Y., A dually active anthrax vaccine that confers protection against both bacilli and toxins (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (19), pp. 10925-10930. , DOI 10.1073/pnas.1834478100; 
Schneerson, R., Kubler-Kielb, J., Liu, T.-Y., Dai, Z.-D., Leppla, S.H., Yergey, A., Backlund, P., Robbins, J.B., Poly(γ-D-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: A potential addition to the anthrax vaccine (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8945-8950. , DOI 10.1073/pnas.1633512100; 
Prescott, A.G., Stock II, L.R., (2005) Method for Producing Medical and Commercial Grade Poly-γ-glutamic Acid of High Molecular Weight, , U. S. Patent 20050095679; 
Prodhomme, E.J.F., Tutt, A.L., Glennie, M.J., Bugg, T.D.H., Multivalent Conjugates of Poly-γ-D-glutamic Acid from Bacillus licheniformis with Antibody F(abβ) and Glycopeptide Ligands (2003) Bioconjugate Chemistry, 14 (6), pp. 1148-1155. , DOI 10.1021/bc020019m; 
Sutherland, M.D., Thorkildson, P., Parks, S.D., Kozel, T.R., In vivo fate and distribution of poly-γ-D-glutamic acid, the capsular antigen from Bacillus anthracis (2008) Infection and Immunity, 76 (3), pp. 899-906. , DOI 10.1128/IAI.01176-07; 
Serizawa, T., Goto, H., Kishida, A., Endo, T., Akashi, M., Improved alternate deposition of biodegradable naturally occurring polymers onto a quartz crystal microbalance (1999) J. Polym. Sci., Part A: Polym. Chem., 37, pp. 801-804; 
Tachaboonyakiat, W., Serizawa, T., Endo, T., Akashi, M., The influence of molecular weight over the ultrathin films of biodegradable polyion complexes between chitosan and poly(γ-glutamic acid) (2000) Polym. J., 32, pp. 481-485; 
Hsieh, C.Y., Tsai, S.P., Wang, D.M., Chang, Y.N., Hsieh, H.J., Preparation of γ-PGA/chitosan composite tissue engineering matrices (2005) Biomaterials, 26, pp. 5617-5623; 
Kang, H.S., Park, S.H., Lee, Y.G., Son, T.I., Polyelectrolyte complex hydrogel composed of chitosan and poly(γ-glutamic acid) for biological application: Preparation, physical properties, and cytocompatibility (2007) J. Appl. Polym. Sci., 103, pp. 386-394; 
Tsao, C.T., Chang, C.H., Lin, Y.Y., Wu, M.F., Wang, J.L., Han, J.L., Hsieh, K.H., Antibacterial activity and biocompatibility of a chitosan-γ- poly(glutamic acid) polyelectrolyte complex hydrogel (2010) Carbohydr. Res., 345, pp. 1774-1780; 
Hajdu, I., Bodnar, M., Filipcsei, G., Hartmann, J.F., Daroczi, L., Zrinyi, M., Borbely, J., Nanoparticles prepared by self-assembly of chitosan and poly-γ-glutamic acid (2008) Colloid Polym. Sci., 286, pp. 343-350; 
Lin, Y.H., Chung, C.K., Chen, C.T., Liang, H.F., Chen, S.C., Sung, H.W., Preparation of nanoparticles composed of chitosan/poly-γ-glutamic acid and evaluation of their permeability through Caco-2 cells (2005) Biomacromolecules, 6, pp. 1104-1112; 
Imoto, T., Kida, T., Matsusaki, M., Akashi, M., Preparation and unique pH-responsive properties of novel biodegradable nanocapsules composed of poly(γ-glutamic acid) and chitosan as weak polyelectrolytes (2010) Macromol. Biosci., 10, pp. 271-277; 
Crompton, K.E., Goud, J.D., Bellamkonda, R.V., Gengenbach, T.R., Finkelstein, D.I., Horne, M.K., Forsythe, J.S., Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering (2007) Biomaterials, 28 (3), pp. 441-449. , DOI 10.1016/j.biomaterials.2006.08.044, PII S0142961206007642; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., Molecular parameters of chitosans depolymerized with the aid of papain (1996) Carbohydrate Polymers, 29 (1), pp. 63-68. , DOI 10.1016/0144-8617(95)00147-6; 
Kunioka, M., Goto, A., Biosynthesis of poly(γ-glutamic acid) from L-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335 (1994) Applied Microbiology and Biotechnology, 40 (6), pp. 867-872. , DOI 10.1007/s002530050082; 
Kubota, H., Matsunobu, T., Uotani, K., Takebe, H., Satoh, A., Tanaka, T., Taniguchi, M., Production of poly(γ-glutamic acid) by Bacillus subtilis F-2-01 (1993) Biosci., Biotechnol., Biochem., 57, pp. 1212-1213; 
Ashiuchi, M., Soda, K., Misono, H., A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336: Gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells (1999) Biochemical and Biophysical Research Communications, 263 (1), pp. 6-12. , DOI 10.1006/bbrc.1999.1298; 
Yamaguchi, F., Ogawa, Y., Kikuchi, M., Yuasa, K., Motai, H., Detection of γ-polyglutamic acid (γ-PGA) by SDS-PAGE (1996) Biosci., Biotechnol., Biochem., 60, pp. 255-258; 
Scolnik, Y., Portnaya, I., Cogan, U., Tal, S., Haimovitz, R., Fridkin, M., Elitzur, A.C., Shinitzky, M., Subtle differences in structural transitions between poly-L-and poly-D-amino acids of equal length in water (2006) Phys. Chem. Chem. Phys., 8, pp. 333-339; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) Journal of Biomedical Materials Research - Part A, 67 (1), pp. 158-171; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Da Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Transactions on Magnetics, 35 (5 PART 3), pp. 4361-4367. , DOI 10.1109/20.799086; 
Ambrosio, L., Borzacchiello, A., Netti, P.A., Nicolais, L., Properties of new materials: Rheological study on hyaluronic acid and its derivative solutions (1999) Journal of Macromolecular Science - Pure and Applied Chemistry, 36 A (7-8), pp. 991-1000; 
Horcas, I., Fernandez, R., Gomez-Rodriguez, J.M., Colchero, J., Gomez-Herrero, J., Baro, A.M., WSXM: A software for scanning probe microscopy and a tool for nanotechnology (2007) Rev. Sci. Instrum., 78, p. 1; 
Martins, M.C.L., Ochoa-Mendes, V., Ferreira, G., Barbosa, J.N., Curtin, S.A., Ratner, B.D., Barbosa, M.A., Interactions of leukocytes and platelets with poly(lysine/leucine) immobilized on tetraethylene glycol-terminated self-assembled monolayers (2011) Acta Biomater., 7, pp. 1949-1955; 
Werner, C., Konig, U., Augsburg, A., Arnhold, C., Korber, H., Zimmermann, R., Jacobasch, H.-J., Electrokinetic surface characterization of biomedical polymers - A survey (1999) Colloids and Surfaces A: Physicochemical and Engineering Aspects, 159 (2-3), pp. 519-529. , DOI 10.1016/S0927-7757(99)00290-3, PII S0927775799002903; 
Fairbrother, F., Mastin, H., Studies in electro-endosmosis part I (1924) J. Chem. Soc., 125, pp. 2319-2330; 
Voinova, M.V., Rodahl, M., Jonson, M., Kasemo, B., Viscoelastic acoustic response of layered polymer films at fluid-solid interfaces: Continuum mechanics approach (1999) Phy. Scr., 59, pp. 391-396; 
Hook, F., Kasemo, B., Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking: A quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study (2001) Analytical Chemistry, 73 (24), pp. 5796-5804. , DOI 10.1021/ac0106501; 
Hook, F., Rodahl, M., Brzezinski, P., Kasemo, B., Measurements using the quartz crystal microbalance technique of ferritin monolayers on methyl-thiolated gold: Dependence of energy dissipation and saturation coverage on salt concentration (1998) Journal of Colloid and Interface Science, 208 (1), pp. 63-67. , DOI 10.1006/jcis.1998.5774; 
Larsson, C., Rodahl, M., Hook, F., Characterization of DNA immobilization and subsequent hybridization on a 2D arrangement of streptavidin on a biotin-modified lipid bilayer supported on SiO 2 (2003) Analytical Chemistry, 75 (19), pp. 5080-5087. , DOI 10.1021/ac034269n; 
Sauerbrey, G., Verwendung von schwingquarzen zur wagung dunner schichten und zur mikrowagung (1959) Z. Phys., 155, pp. 206-222; 
Schlenoff, J.B., Dubas, S.T., Mechanism of polyelectrolyte multilayer growth: Charge overcompensation and distribution (2001) Macromolecules, 34 (3), pp. 592-598. , DOI 10.1021/ma0003093; 
Kim, D.K., Han, S.W., Kim, C.H., Hong, J.D., Kim, K., Morphology of multilayers assembled by electrostatic attraction of oppositely charged model polyelectrolytes (1999) Thin Solid Films, 350, pp. 153-160; 
Picart, C., Lavalle, Ph., Hubert, P., Cuisinier, F.J.G., Decher, G., Schaaf, P., Voegel, J.-C., Buildup mechanism for poly(L-lysine)/hyaluronic acid films onto a solid surface (2001) Langmuir, 17 (23), pp. 7414-7424. , DOI 10.1021/la010848g; 
Richert, L., Lavalle, P., Payan, E., Shu, X.Z., Prestwich, G.D., Stoltz, J.F., Schaaf, P., Picart, C., Layer by layer buildup of polysaccharide films: Physical chemistry and cellular adhesion aspects (2004) Langmuir, 20, pp. 448-458; 
Tsao, C.T., Hang, C.H., Lin, Y.Y., Wu, M.F., Wang, J.L., Young, T.H., Han, J.L., Hsieh, K.H., Evaluation of chitosan/γ-poly(glutamic acid) polyelectrolyte complex for wound dressing materials (2011) Carbohydr. Polym., 84, pp. 812-819; 
Bonazzola, C., Calvo, E.J., A Fourier transform infrared reflection-absorption spectroscopy study of redox polyelectrolyte films (2003) Langmuir, 19, pp. 5279-5286; 
Boulmedais, F., Ball, V., Schwinte, P., Frisch, B., Schaaf, P., Voegel, J.C., Buildup of exponentially growing multilayer polypeptide films with internal secondary structure (2003) Langmuir, 19, pp. 440-445; 
Boulmedais, F., Bozonnet, M., Schwinte, P., Voegel, J.C., Schaaf, P., Multilayered polypeptide films: Secondary structures and effect of various stresses (2003) Langmuir, 19, pp. 9873-9882; 
He, L.M., Neu, M.P., Vanderberg, L.A., Bacillus lichenformis γ-glutamyl exopolymer: Physicochemical characterization and U(VI) interaction (2000) Environmental Science and Technology, 34 (9), pp. 1694-1701. , DOI 10.1021/es991119e; 
Chan, Y.H.M., Schweiss, R., Werner, C., Grunze, M., Electrokinetic characterization of oligo-and poly(ethylene glycol)-terminated self-assembled monolayers on gold and glass surfaces (2003) Langmuir, 19, pp. 7380-7385; 
Giesbers, M., Kleijn, J.M., Cohen Stuart, M.A., The electrical double layer on gold probed by electrokinetic and surface force measurements (2002) Journal of Colloid and Interface Science, 248 (1), pp. 88-95. , DOI 10.1006/jcis.2001.8144; 
Schlenoff, J.B., Ly, H., Li, M., Charge and mass balance in polyelectrolyte multilayers (1998) Journal of the American Chemical Society, 120 (30), pp. 7626-7634. , DOI 10.1021/ja980350+; 
Rodahl, M., Hook, F., Fredriksson, C., Keller, C.A., Krozer, A., Brzezinski, P., Voinova, M., Kasemo, B., Simultaneous frequency and dissipation factor QCM measurements of biomolecular adsorption and cell adhesion (1997) Faraday Discussions, 107, pp. 229-246; 
Guzman, E., San Miguel, V., Peinado, C., Ortega, F., Rubio, R.C., Polyelectrolyte multilayers containing triblock copolymers of different charge ratio (2010) Langmuir, 26, pp. 11494-11502; 
Salomaki, M., Laiho, T., Kankare, J., Counteranion-controlled properties of polyelectrolyte multilayers (2004) Macromolecules, 37 (25), pp. 9585-9590. , DOI 10.1021/ma048701u; 
Dutta, A.K., Nayak, A., Belfort, G., Viscoelastic properties of adsorbed and cross-linked polypeptide and protein layers at a solidliquid interface (2008) J. Colloid Interface Sci., 324, pp. 55-60
},
	month = {2011///},
	pages = {4183 - 4195},
	abstract = {Chitosan (Ch) is a nontoxic and biocompatible polysaccharide extensively used in biomedical applications. Ch, as a polycation, can be combined with anionic polymers by layer-bylayer (LbL) self-assembly, giving rise to multilayered complexed architectures. These structures can be used in tissue engineering strategies, as drug delivery systems, or artificial matrices mimicking the extracellular microenvironment. In this work, Ch was combined with poly(γ-glutamic acid) (γ-PGA). γ-PGA is a polyanion, which was microbially produced, and is known for its low immunogenic reaction and low cytotoxicity. Multilayered ultrathin films were assembled by LbL, with a maximum of six layers. The interaction between both polymers was analyzed by: ellipsometry, quartz crystal microbalance with dissipation, Fourier transform infrared spectroscopy, atomic force microscopy, and zeta potential measurements. Ch/γ-PGA polyelectrolyte multilayers (PEMs) revealed no cytotoxicity according to ISO 10993-5. Overall, this study demonstrates that Ch can interact electrostatically with γ-PGA forming multilayered films. Furthermore, this study provides a comprehensive characterization of Ch/γ-PGA PEM structures, elucidating the contribution of each layer for the nanostructured films. These model surfaces can be useful substrates to study cell-biomaterial interactions in tissue regeneration. {\textcopyright} 2011 American Chemical Society.},
	keywords = {Amino acids, Anionic polymers, article, Atomic force microscopy, Atomic spectroscopy, Biocompatible Materials, Biocompatible polysaccharides, Biomaterials, Biomedical applications, Cell-biomaterial interactions, chemical interaction, chitosan, controlled study, Cytotoxicity, Drug delivery, drug delivery system, Drug Delivery Systems, Electrolytes, ellipsometry, enzyme activity, Extracellular, Fourier transform infrared spectroscopy, Glutamic acid, immunogenicity, infrared spectroscopy, Layer by layer self assembly, Medical applications, microenvironment, Microenvironments, Microscopy, Atomic Force, Model surface, Models, Molecular, Multi-layered, Multi-layered films, Multilayers, nanofilm, Nanostructured Films, polyanion, Polyanions, Polycations, polyelectrolyte, Polyelectrolyte complexes, Polyelectrolyte multilayer, Polyelectrolytes, Polyethylenes, polyglutamic acid, Polymers, priority journal, Programmed control systems, Quartz, quartz crystal microbalance, Quartz crystal microbalance with dissipation, Self assembly, Tissue, Tissue engineering, Tissue regeneration, Ultrathin films, Viscoelastic Substances, Zeta potential, Zeta potential measurements},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84856258191\&partnerID=40\&md5=8ba447b7a45e8004e5b3aa3d5cff2404},
	author = {Antunes, J.C. and Pereira, C.L. and Molinos, M. and Ferreira-da-Silva, F. and Dessi, M. and Gloria, A. and Ambrosio, L. and Gonca̧lves, R.M. and Barbosa, M.A.}
}
@article {2231,
	title = {Molecular recognition force spectroscopy: A new tool to tailor targeted nanoparticles},
	journal = {SmallSmall},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1002/smll.201002074Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis̃o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Verma, I.M., Weitzman, M.D., (2005) Annu. Rev. Biochem., 74, pp. 711-738;Midoux, P., Breuzard, G., Gomez, J.P., Pichon, C., (2008) Curr. Gene Ther., 8, pp. 335-352; 
Oliveira, H., Fernandez, R., Pires, L.R., Martins, M.C., Simoes, S., Barbosa, M.A., Pego, A.P., (2010) J. Controlled Release, 143, pp. 350-358; 
Hinterdorfer, P., Baumgartner, W., Gruber, H.J., Schilcher, K., Schindler, H., (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 3477-3481; 
Ebner, A., Wildling, L., Kamruzzahan, A.S., Rankl, C., Wruss, J., Hahn, C.D., Holzl, M., Gruber, H.J., (2007) Bioconjugate Chem., 18, pp. 1176-1184; 
Fotinou, C., Emsley, P., Black, I., Ando, H., Ishida, H., Kiso, M., Sinha, K.A., Isaacs, N.W., (2001) J. Biol. Chem., 276, pp. 32274-32281; 
Ebner, A., Hinterdorfer, P., Gruber, H.J., (2007) Ultramicroscopy, 107, pp. 922-927; 
Baumgartner, W., Hinterdorfer, P., Schindler, H., (2000) Ultramicroscopy, 82, pp. 85-95; 
Evans, E., Ritchie, K., (1997) Biophys. J., 72, pp. 1541-1555; 
Evans, E., Ritchie, K., (1999) Biophys. J., 76, pp. 2439-2447; 
Bell, G.I., (1978) Science, 200, pp. 618-627; 
Sinha, K., Box, M., Lalli, G., Schiavo, G., Schneider, H., Groves, M., Siligardi, G., Fairweather, N., (2000) Mol. Microbiol., 37, pp. 1041-1051; 
Halpern, J.L., Loftus, A., (1993) J. Biol. Chem., 268, pp. 11188-11192; 
Owens III, D.E., Peppas, N.A., (2006) Int. J. Pharm., 307, pp. 93-102; 
Storm, G., Belliot, S.O., Daemen, T., Lasic, D.D., (1995) Adv. Drug Delivery Rev., 17, pp. 31-48; 
Gu, F., Zhang, L., Teply, B.A., Mann, N., Wang, A., Radovic-Moreno, A.F., Langer, R., Farokhzad, O.C., (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 2586-2591; 
Butt, H.J., Jaschke, M., (1995) Nanotechnology, 6, pp. 1-7; 
Hutter, J.L., Bechhoefer, J., (1993) Rev. Sci. Instrum., 64, pp. 1868-1873; 
Rankl, C., Kienberger, F., Wildling, L., Wruss, J., Gruber, H.J., Blaas, D., Hinterdorfer, P., (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 17778-17783
},
	month = {2011///},
	pages = {1236 - 1241},
	abstract = {The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine. A novel screening tool is described to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. The unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. {\textcopyright} 2011 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {article, Atomic force microscopy, Atomic spectroscopy, atomic-force microscopy, chemistry, Event probability, Force spectroscopy, Functionalized nanoparticles, gene technology, Gene-delivery systems, Genes, Medical nanotechnology, medicine, methodology, Microscopy, Atomic Force, Molecular biology, Molecular recognition, molecular recognition force spectroscopy, Nanomedicines, nanoparticle, Nanoparticle formulation, nanoparticles, Nanotechnology, Neuronal cell, Optimization, Receptor interaction, Screening tool, Specific interaction, Targeted nanoparticle},
	isbn = {16136810 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955561627\&partnerID=40\&md5=85fa523edfcd7a6e171a53820be7f230},
	author = {Oliveira, H. and Rangl, M. and Ebner, A. and Mayer, B. and Hinterdorfer, P. and P{\^e}go, A.P.}
}
@article {2237,
	title = {Pectin-based injectable biomaterials for bone tissue engineering},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {12},
	year = {2011},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm101110xPubMed ID: 21302960Language of Original Document: EnglishCorrespondence Address: Munarin, F.; Biomatlab, Bioengineering Department, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milan, Italy; email: fabiola.munarin@mail.polimi.itChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; osteocalcin, 136461-80-8; pectin, 9000-69-5; Biocompatible Materials; Oligopeptides; Pectins; arginyl-glycyl-aspartic acid, 99896-85-2; pectin, 9000-69-5References: Brown, M.J., Lester, J.N., (1980) Appl. Environ. Microbiol., 40, pp. 179-185;Knox, J.P., (2008) Curr. Opin. Plant Biol., 1, pp. 308-313; 
Jarvis, M.C., (1984) Plant, Cell Environ, 7, pp. 153-164; 
Thom, D., Grant, G.T., Morris, E.R., Rees, D.A., (1982) Carbohydr. Res., 100, pp. 29-42; 
Fang, Y., Al-Assaf, S., Phillips, G.O., Nishinari, K., Funami, T., Williams, P.A., (2008) Carbohydr. Polym., 72, pp. 334-341; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., (1999) Biomaterials, 20, pp. 45-53; 
Price, L.S., (1997) Bioessays, 19, pp. 941-943; 
Dee, K.C., Andersen, T.T., Bizios, R., (1999) Biomaterials, 20, pp. 221-227; 
Pierschbacher, M.D., Ruoslahti, E., (1984) Nature, 309, pp. 30-33; 
Ruoslahti, E., Pierschbacher, M., (1987) Science, 238, pp. 491-497; 
Hynes, R.O., (1992) Cell, 69, pp. 11-25; 
Yamada, K.M., (1997) Matrix Biol., 16, pp. 137-141; 
Evangelista, M.B., Hsiong, S.X., Fernandes, R., Sampaio, P., Kong, H., Barrias, C.C., Salema, R., Granja, P.L., (2007) Biomaterials, 28, pp. 3644-3655; 
Grellier, M., Granja, P.L., Fricain, J., Bidarra, S.J., Renard, M., Bareille, R., Bourget, C., Barbosa, M.A., (2009) Biomaterials, 30, pp. 3271-3278; 
Liu, L., Fishman, M.L., Kost, J., Hicks, K.B., (2003) Biomaterials, 24, pp. 3333-3343; 
Rao, K.P., Prabhashankar, B., Kumar, A., Khan, A., Biradar, S.S., Srishail, S.P., Satyanath, B., (2003) Yale J. Biol. Med., 76, pp. 149-154; 
Jackson, C.L., Dreaden, T.M., Theobald, L.K., Tran, N.M., Beal, T.L., Eid, M., Gao, M.Y., Mohnen, D., (2007) Glycobiology, 17, pp. 805-819; 
Glinsky, V.V., Raz, A., (2009) Carbohydr. Res., 344, pp. 1788-1791; 
Vayssade, M., Sengkhamparn, N., Verhoef, R., Delaigue, C., Goundiam, O., Vigneron, P., Voragen, A.G., Nagel, M.D., (2010) Phytother. Res., 24, pp. 982-989; 
Katav, T., Liu, L., Traitel, T., Goldbart, R., Wolfson, M., Kost, J., (2008) J. Controlled Release, 130, pp. 183-191; 
Opanasopit, P., Apirakaramwong, A., Ngawhirunpat, T., Rojanarata, T., Ruktanonchai, U., (2008) AAPS PharmSciTech, 9, pp. 67-74; 
Morra, M., Cassinelli, C., Cascardo, G., Nagel, M., Della Volpe, C., Siboni, S., Maniglio, D., Ulvskov, P., (2004) Biomacromolecules, 5, pp. 2094-2104; 
Bussy, C., Verhoef, R., Haeger, A., Morra, M., Duval, J., Vigneron, P., Bensoussan, A., Nagel, M.D., (2008) J. Biomed. Mater. Res. A, 86, pp. 597-606; 
Kokkonen, H.E., Ilvesaro, J.M., Morra, M., Schols, H.A., Tuukkanen, J., (2007) Biomacromolecules, 8, pp. 509-515; 
Munarin, F., Giuliano, L., Bozzini, S., Tanzi, M.C., Petrini, P., (2010) Mater. Sci. Eng. C, 30, pp. 491-496; 
Karageorgiou, V., Kaplan, D., (2005) Biomaterials, 26, pp. 5474-5491; 
Volkmer, E., Drosse, I., Otto, S., Stangelmayer, A., Stengele, M., Kallukalam, B.C., Mutschler, W., Schieker, M., (2008) Tissue Eng., Part A, 14, pp. 1331-1340; 
Anselme, K., (2000) Biomaterials, 21, pp. 667-681; 
Hersel, U., Dahmen, C., Kessler, H., (2003) Biomaterials, 24, pp. 4385-4415; 
Stains, J.P., Civitelli, R., (2005) Birth Defects Res., Part C, 75, pp. 72-80; 
Ichibouji, T., Miyazaki, T., Ishida, E., Ashizuka, M., Sugino, A., Ohtsuki, C., Kuramoto, K., (2008) J. Ceram. Soc. Jpn., 116, pp. 74-78
},
	month = {2011///},
	pages = {568 - 577},
	abstract = {A variety of natural polymers and proteins are considered to be 3D cell culture structures able to mimic the extracellular matrix (ECM) to promote bone tissue regeneration. Pectin, a natural polysaccharide extracted from the plant cell walls and having a chemical structure similar to alginate, provides interesting properties as artificial ECM. In this work, for the first time, pectin, modified with an RGD-containing oligopeptide or not, is used as an ECM alternative to immobilize cells for bone tissue regeneration. The viability, metabolic activity, morphology, and osteogenic differentiation of immobilized MC3T3-E1 preosteoblats demonstrate the potential of this polysaccharide to keep immobilized cells viable and differentiating. Preosteoblasts immobilized in both types of pectin microspheres maintained a constant viability up to 29 days and were able to differentiate. The grafting of the RGD peptide on pectin backbone induced improved cell adhesion and proliferation within the microspheres. Furthermore, not only did cells grow inside but also theywere able to spread out from themicrospheres and to organize themselves in 3D structures producing a mineralized extracellular matrix. These promising results suggest that pectin can be proposed as an injectable cell vehicle for bone tissue regeneration. {\textcopyright} 2011 American Chemical Society.},
	keywords = {3-D Cell culture, 3D Structure, 3T3 Cells, Adhesion, Alginate, animal cell, Animals, arginylglycylaspartic acid, article, Biocompatible Materials, Biopolymers, Bone, Bone and Bones, Bone Regeneration, bone tissue, Bone tissue engineering, Bone tissue regeneration, carbohydrate analysis, cell adhesion, Cell culture, cell differentiation, cell growth, cell metabolism, cell proliferation, cell spreading, cell structure, cell viability, Cells, Chemical modification, Chemical structure, controlled study, Cryoelectron Microscopy, Extracellular matrices, extracellular matrix, gene expression, immobilized cell, Immobilized cells, Injectable biomaterials, Injections, MC3T3-E1, Metabolic activity, Mice, Microscopy, Electron, Scanning, microsphere, Microspheres, Mineralized extracellular matrixes, mouse, Natural polysaccharide, nonhuman, Oligopeptides, osteoblast, osteocalcin, Osteogenic differentiation, pectin, Pectins, Plant cell culture, Plant cell wall, Preosteoblasts, priority journal, RGD peptide, Three dimensional, Tissue engineering},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955409005\&partnerID=40\&md5=277a7d5e91116f9a03f144aabda0dd96},
	author = {Munarin, F. and Guerreiro, S.G. and Grellier, M.A. and Tanzi, M.C. and Barbosa, M.A. and Petrini, P. and Granja, P.L.}
}
@article {2223,
	title = {Simulations of the NK cell immune synapse reveal that activation thresholds can be established by inhibitory receptors acting locally},
	journal = {Journal of ImmunologyJ. Immunol.},
	volume = {187},
	year = {2011},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: JOIMAdoi: 10.4049/jimmunol.1002208PubMed ID: 21690326Language of Original Document: EnglishCorrespondence Address: Mehr, R.; Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel; email: ramit.mehr@biu.ac.ilChemicals/CAS: Vav protein, 282122-55-8; intercellular adhesion molecule 1, 126547-89-5; H-2 Antigens; Histocompatibility Antigens Class I; Intercellular Adhesion Molecule-1, 126547-89-5; KLRK1 protein, human; Lymphocyte Function-Associated Antigen-1; MHC class I-related chain A; NK Cell Lectin-Like Receptor Subfamily A; NK Cell Lectin-Like Receptor Subfamily K; Receptors, KIRReferences: Burgess, S.J., Maasho, K., Masilamani, M., Narayanan, S., Borrego, F., Coligan, J.E., The NKG2D receptor: Immunobiology and clinical implications (2008) Immunol. Res., 40, pp. 18-34;Gonz{\'a}lez, S., L{\'o}pez-Soto, A., Suarez-Alvarez, B., L{\'o}pez-V{\'a}zquez, A., L{\'o}pez-Larrea, C., NKG2D ligands: Key targets of the immune response (2008) Trends Immunol., 29, pp. 397-403; 
Vyas, Y.M., Maniar, H., Dupont, B., Cutting edge: Differential segregation of the Src homology 2-containing protein tyrosine phosphatase-1 within the early NK cell immune synapse distinguishes noncytolytic from cytolytic interactions (2002) Journal of Immunology, 168 (7), pp. 3150-3154; 
Long, E.O., Negative signaling by inhibitory receptors: The NK cell paradigm (2008) Immunol. Rev., 224, pp. 70-84; 
Ljunggren, H.G., K{\"a}rre, K., In search of the {\textquoteright}missing self{\textquoteright}: MHC molecules and NK cell recognition (1990) Immunol. Today, 11, pp. 237-244; 
Kane, K.P., Silver, E.T., Hazes, B., Specificity and function of activating Ly-49 receptors (2001) Immunol. Rev., 181, pp. 104-114; 
Lanier, L.L., Up on the tightrope: Natural killer cell activation and inhibition (2008) Nat. Immunol., 9, pp. 495-502; 
Radaev, S., Sun, P.D., Structure and function of natural killer cell surface receptors (2003) Annu. Rev. Biophys. Biomol. Struct., 32, pp. 93-114; 
Vivier, E., Nunes, J.A., Vely, F., Natural killer cell signaling pathways (2004) Science, 306 (5701), pp. 1517-1519. , DOI 10.1126/science.1103478; 
Yokoyama, W.M., Plougastel, B.F., Immune functions encoded by the natural killer gene complex (2003) Nat. Rev. Immunol., 3, pp. 304-316; 
Stebbins, C.C., Watzl, C., Billadeau, D.D., Leibson, P.J., Burshtyn, D.N., Long, E.O., Vav1 dephosphorylation by the tyrosine phosphatase SHP-1 as amechanism for inhibition of cellular cytotoxicity (2003) Mol. Cell. Biol., 23, pp. 6291-6299; 
Bryceson, Y.T., Long, E.O., Line of attack: NK cell specificity and integration of signals (2008) Curr. Opin. Immunol., 20, pp. 344-352; 
McCann, F.E., Suhling, K., Carlin, L.M., Eleme, K., Taner, S.B., Yanagi, K., Vanherberghen, B., Davis, D.M., Imaging immune surveillance by T cells and NK cells (2002) Immunol. Rev., 189, pp. 179-192; 
Davis, D.M., Assembly of the immunological synapse for T cells and NK cells (2002) Trends in Immunology, 23 (7), pp. 356-363. , DOI 10.1016/S1471-4906(02)02243-3, PII S1471490602022433; 
Vyas, Y.M., Mehta, K.M., Morgan, M., Maniar, H., Butros, L., Jung, S., Burkhardt, J.K., Dupont, B., Spatial organization of signal transduction molecules in the NK cell immune synapses during MHC class I-regulated noncytolytic and cytolytic interactions (2001) Journal of Immunology, 167 (8), pp. 4358-4367; 
Vyas, Y.M., Maniar, H., Lyddane, C.E., Sadelain, M., Dupont, B., Ligand binding to inhibitory killer cell Ig-like receptors induce colocalization with Src homology domain 2-containing protein tyrosine phosphatase 1 and interruption of ongoing activation signals (2004) Journal of Immunology, 173 (3), pp. 1571-1578; 
Orange, J.S., Formation and function of the lytic NK-cell immunological synapse (2008) Nat. Rev. Immunol., 8, pp. 713-725; 
Eleme, K., Taner, S.B., Onfelt, B., Collinson, L.M., McCann, F.E., Chalupny, N.J., Cosman, D., Davis, D.M., Cell surface organization of stress-inducible proteins ULBP and MICA that stimulate human NK cells and T cells via NKG2D (2004) J. Exp. Med., 199, pp. 1005-1010; 
Cebo, C., Da, R.S., Wittnebel, S., Turhan, A.G., Abdelali, J., Caillat-Zucman, S., Bourhis, J.H., Caignard, A., The decreased susceptibility of Bcr/Abl targets to NK cell-mediated lysis in response to imatinib mesylate involves modulation of NKG2D ligands, GM1 expression, and synapse formation (2006) Journal of Immunology, 176 (2), pp. 864-872; 
Burack, W.R., Lee, K.H., Holdorf, A.D., Dustin, M.L., Shaw, A.S., Cutting edge: Quantitative imaging of raft accumulation in the immunological synapse (2002) J. Immunol., 169, pp. 2837-2841; 
Harder, T., Engelhardt, K.R., Membrane domains in lymphocytes - From lipid rafts to protein scaffolds (2004) Traffic, 5, pp. 265-275; 
Laude, A.J., Prior, I.A., Plasma membrane microdomains: Organization, function and trafficking (2004) Mol. Membr. Biol., 21, pp. 193-205; 
Lou, Z., Jevremovic, D., Billadeau, D.D., Leibson, P.J., A balance between positive and negative signals in cytotoxic lymphocytes regulates the polarization of lipid rafts during the development of cell-mediated killing (2000) J. Exp. Med., 191, pp. 347-354; 
Pike, L.J., Lipid rafts: Bringing order to chaos (2003) Journal of Lipid Research, 44 (4), pp. 655-667. , DOI 10.1194/jlr.R200021-JLR200; 
Pizzo, P., Viola, A., Lymphocyte lipid rafts: Structure and function (2003) Curr. Opin. Immunol., 15, pp. 255-260; 
Rodgers, W., Farris, D., Mishra, S., Merging complexes: Properties of membrane raft assembly during lymphocyte signaling (2005) Trends Immunol., 26, pp. 97-103; 
Schmitz, G., Grandl, M., Update on lipid membrane microdomains (2008) Curr. Opin. Clin. Nutr. Metab. Care, 11, pp. 106-112; 
Wright, M.D., Moseley, G.W., Van Spriel, A.B., Tetraspanin microdomains in immune cell signalling and malignant disease (2004) Tissue Antigens, 64 (5), pp. 533-542. , DOI 10.1111/j.1399-0039.2004.00321.x; 
Vogt, A.B., Spindeldreher, S., Kropshofer, H., Clustering of MHCpeptide complexes prior to their engagement in the immunological synapse: Lipid raft and tetraspan microdomains (2002) Immunol. Rev., 189, pp. 136-151; 
Lee, S.J.E., Hori, Y., Groves, J.T., Dustin, M.L., Chakraborty, A.K., Correlation of a dynamic model for immunological synapse formation with effector functions: Two pathways to synapse formation (2002) Trends Immunol., 23, pp. 492-499; 
Giagulli, C., Scarpini, E., Ottoboni, L., Narumiya, S., Butcher, E.C., Constantin, G., Laudanna, C., RhoA and zeta PKC Control Distinct Modalities of LFA-1 Activation by Chemokines: Critical Role of LFA-1 Affinity Triggering in Lymphocyte In Vivo Homing (2004) Immunity, 20 (1), pp. 25-35. , DOI 10.1016/S1074-7613(03)00350-9; 
Masilamani, M., Nguyen, C., Kabat, J., Borrego, F., Coligan, J.E., CD94/NKG2A inhibits NK cell activation by disrupting the actin network at the immunological synapse (2006) J. Immunol., 177, pp. 3590-3596; 
Weikl, T.R., Lipowsky, R., Pattern formation during T-cell adhesion (2004) Biophysical Journal, 87 (6), pp. 3665-3678. , DOI 10.1529/biophysj.104.045609; 
Kotzer, S., Salmon-Divon, M., Kaplan, A., Almeida, C.R., Hoglund, P., Davis, D.M., Mehr, R., Modeling the influence of molecule and cell surface micro-domain distribution on the formation of T cell immunological synapses (2007) Life Science Systems and Applications Workshop, 2007. LISA 2007. IEEE/NIH. 8-9 November, , http://ieeexplore.ieee.org/xpl/freeabs_all.jsp?arnumber=4400878, Available at; 
Culley, F.J., Johnson, M., Evans, J.H., Kumar, S., Crilly, R., Casasbuenas, J., Schnyder, T., Ushakov, D.S., Natural killer cell signal integration balances synapse symmetry and migration (2009) PLoS Biol., 7, pp. e1000159; 
Treanor, B., Lanigan, P.M.P., Kumar, S., Dunsby, C., Munro, I., Auksorius, E., Culley, F.J., Neil, M.A.A., Microclusters of inhibitory killer immunoglobulin-like receptor signaling at natural killer cell immunological synapses (2006) J. Cell Biol., 174, pp. 153-161; 
Back, J., Chalifour, A., Scarpellino, L., Held, W., Stable masking by H-2Dd cis ligand limits Ly49A relocalization to the site of NK cell/target cell contact (2007) Proc. Natl. Acad. Sci. USA, 104, pp. 3978-3983; 
Back, J., Scarpellino, L., Held, W., Probing the interactions of NK cell receptors with ligand expressed in trans and cis (2010) MethodsMol. Biol., 612, pp. 313-323; 
Chalifour, A., Scarpellino, L., Back, J., Brodin, P., Dev{\`e}vre, E., Gros, F., L{\'e}vy, F., Held, W., A role for cis interaction between the inhibitory Ly49A receptor and MHC class I for natural killer cell education (2009) Immunity, 30, pp. 337-347; 
Doucey, M.A., Scarpellino, L., Zimmer, J., Guillaume, P., Luescher, I.F., Bron, C., Held, W., Cis association of Ly49A with MHC class I restricts natural killer cell inhibition (2004) Nat. Immunol., 5, pp. 328-336; 
Held, W., Mariuzza, R.A., Cis interactions of immunoreceptors with MHC and non-MHC ligands (2008) Nat. Rev. Immunol., 8, pp. 269-278; 
Scarpellino, L., Oeschger, F., Guillaume, P., Coedert, J.D., Levy, F., Leclercq, G., Held, W., Interactions of Ly49 family receptors with MHC class I ligands in trans and cis (2007) Journal of Immunology, 178 (3), pp. 1277-1284; 
Saffman, P.G., Delbr{\"u}ck, M., Brownian motion in biological membranes (1975) Proc. Natl. Acad. Sci. USA, 72, pp. 3111-3113; 
Raychaudhuri, S., Chakraborty, A.K., Kardar, M., Effective membrane model of the immunological synapse (2003) Phys. Rev. Lett., 91, p. 208101; 
Burroughs, N.J., W{\"u}lfing, C., Differential segregation in a cell-cell contact interface: The dynamics of the immunological synapse (2002) Biophys. J., 83, pp. 1784-1796; 
Coombs, D., Goldstein, B., Effects of the geometry of the immunological synapse on the delivery of effector molecules (2004) Biophysical Journal, 87 (4), pp. 2215-2220. , DOI 10.1529/biophysj.104.045674; 
K{\"o}hler, K., Xiong, S., Brzostek, J., Mehrabi, M., Eissmann, P., Harrison, A., Cordoba, S.P., Gould, K., Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells (2010) PLoS ONE, 5, pp. e15374; 
Dykstra, M., Cherukuri, A., Sohn, H.W., Tzeng, S.J., Pierce, S.K., Location is everything: Lipid rafts and immune cell signaling (2003) Annu. Rev. Immunol., 21, pp. 457-481; 
Horejs{\'\i}, V., The roles of membrane microdomains (rafts) in T cell activation (2003) Immunol. Rev., 191, pp. 148-164; 
Fujiwara, T., Ritchie, K., Murakoshi, H., Jacobson, K., Kusumi, A., Phospholipids undergo hop diffusion in compartmentalized cell membrane (2002) Journal of Cell Biology, 157 (6), pp. 1071-1081. , DOI 10.1083/jcb.200202050; 
Giurisato, E., McIntosh, D.P., Tassi, M., Gamberucci, A., Benedetti, A., T cell receptor can be recruited to a subset of plasma membrane rafts, independently of cell signaling and attendantly to raft clustering (2003) J. Biol. Chem., 278, pp. 6771-6778; 
Kusumi, A., Koyama-Honda, I., Suzuki, K., Molecular dynamics and interactions for creation of stimulation-induced stabilized rafts from small unstable steady-state rafts (2004) Traffic, 5 (4), pp. 213-230. , DOI 10.1111/j.1600-0854.2004.0178.x; 
Marwali, M.R., MacLeod, M.A., Muzia, D.N., Takei, F., Lipid rafts mediate association of LFA-1 and CD3 and formation of the immunological synapse of CTL (2004) Journal of Immunology, 173 (5), pp. 2960-2967; 
Montixi, C., Langlet, C., Bernard, A.M., Thimonier, J., Dubois, C., Wurbel, M.A., Chauvin, J.P., He, H.T., Engagement of T cell receptor triggers its recruitment to low-density detergent-insoluble membrane domains (1998) EMBO J., 17, pp. 5334-5348; 
Munro, S., Lipid rafts: Elusive or illusive? (2003) Cell, 115, pp. 377-388; 
Pizzo, P., Giurisato, E., Bigsten, A., Tassi, M., Tavano, R., Shaw, A., Viola, A., Physiological T cell activation starts and propagates in lipid rafts (2004) Immunol. Lett., 91, pp. 3-9; 
Xavier, R., Brennan, T., Li, Q., McCormack, C., Seed, B., Membrane compartmentation is required for efficient T cell activation (1998) Immunity, 8, pp. 723-732; 
Filipp, D., Julius, M., Lipid rafts: Resolution of the "fyn problem"? (2004) Mol. Immunol., 41, pp. 645-656; 
Schade, A.E., Levine, A.D., Lipid raft heterogeneity in human peripheral blood T lymphoblasts: A mechanism for regulating the initiation of TCR signal transduction (2002) J. Immunol., 168, pp. 2233-2239; 
Janes, P.W., Ley, S.C., Magee, A.I., Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor (1999) J. Cell Biol., 147, pp. 447-461; 
Hiltbold, E.M., Poloso, N.J., Roche, P.A., MHC class II-peptide complexes and APC lipid rafts accumulate at the immunological synapse (2003) J. Immunol., 170, pp. 1329-1338; 
W{\"u}lfing, C., Tskvitaria-Fuller, I., Burroughs, N., Sjaastad, M.D., Klem, J., Schatzle, J.D., Interface accumulation of receptor/ligand couples in lymphocyte activation: Methods, mechanisms, and significance (2002) Immunol. Rev., 189, pp. 64-83; 
Poloso, N.J., Roche, P.A., Association of MHC class II-peptide complexes with plasma membrane lipid microdomains (2004) Curr. Opin. Immunol., 16, pp. 103-107; 
Anderson, H.A., Hiltbold, E.M., Roche, P.A., Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation (2000) Nat. Immunol., 1, pp. 156-162; 
Meyer Zum Bueschenfelde, C.O., Unternaehrer, J., Mellman, I., Bottomly, K., Regulated recruitment of MHC class II and costimulatory molecules to lipid rafts in dendritic cells (2004) J. Immunol., 173, pp. 6119-6124; 
Szollosi, J., Horejsi, V., Bene, L., Angelisova, P., Damjanovich, S., Supramolecular Complexes of MHC Class I, MHC Class II, CD20, and Tetraspan Molecules (CD53, CD81, and CD82) at the Surface of a B Cell Line JY (1996) Journal of Immunology, 157 (7), pp. 2939-2946; 
Leitinger, B., Hogg, N., The involvement of lipid rafts in the regulation of integrin function (2002) Journal of Cell Science, 115 (5), pp. 963-972; 
Cherukuri, A., Cheng, P.C., Pierce, S.K., The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens (2001) J. Immunol., 167, pp. 163-172; 
Sheets, E.D., Holowka, D., Baird, B., Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes (1999) Journal of Cell Biology, 145 (4), pp. 877-887. , DOI 10.1083/jcb.145.4.877; 
Jordan, S., Rodgers, W., T cell glycolipid-enriched membrane domains are constitutively assembled as membrane patches that translocate to immune synapses (2003) Journal of Immunology, 171 (1), pp. 78-87; 
Kaizuka, Y., Douglass, A.D., Vardhana, S., Dustin, M.L., Vale, R.D., The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells (2009) J. Cell Biol., 185, pp. 521-534; 
Dustin, M.L., Membrane domains and the immunological synapse: Keeping T cells resting and ready (2002) J. Clin. Invest., 109, pp. 155-160; 
Almeida, C.R., Davis, D.M., Segregation of HLA-C from ICAM-1 at NK cell immune synapses is controlled by its cell surface density (2006) J. Immunol., 177, pp. 6904-6910; 
Delon, J., The immunological synapse (2000) Curr. Biol., 10, pp. R214; 
Anton Van Der Merwe, P., Davis, S.J., Shaw, A.S., Dustin, M.L., Cytoskeletal polarization and redistribution of cell-surface molecules during T cell antigen recognition (2000) Semin. Immunol., 12, pp. 5-21; 
Bossi, G., Trambas, C., Booth, S., Clark, R., Stinchcombe, J., Griffiths, G.M., The secretory synapse: The secrets of a serial killer (2002) Immunol. Rev., 189, pp. 152-160; 
Endt, J., McCann, F.E., Almeida, C.R., Urlaub, D., Leung, R., Pende, D., Davis, D.M., Watzl, C., Inhibitory receptor signals suppress ligation-induced recruitment of NKG2D to GM1-rich membrane domains at the human NK cell immune synapse (2007) Journal of Immunology, 178 (9), pp. 5606-5611; 
Gasser, S., Raulet, D.H., Activation and self-tolerance of natural killer cells (2006) Immunol. Rev., 214, pp. 130-142; 
Rubio, G., F{\'e}rez, X., S{\'a}nchez-Campillo, M., G{\'a}lvez, J., Mart{\'\i}, S., Verd{\'u}, R., Hern{\'a}ndez-Caselles, T., Garc{\'\i}a-Pe{\~n}arrubia, P., Cross-linking of MHC class I molecules on human NK cells inhibits NK cell function, segregates MHC I from the NK cell synapse, and induces intracellular phosphotyrosines (2004) J. Leukoc. Biol., 76, pp. 116-124; 
Kaizuka, Y., Douglass, A.D., Varma, R., Dustin, M.L., Vale, R.D., Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells (2007) Proc. Natl. Acad. Sci. USA, 104, pp. 20296-20301; 
Brodin, P., Lakshmikanth, T., Mehr, R., Johansson, M.H., Duru, A.D., Achour, A., Salmon-Divon, M., Johansson, S., Natural killer cell tolerance persists despite significant reduction of self MHC class I on normal target cells in mice (2010) PLoS ONE, 5, pp. e1317; 
Carlin, L.M., Eleme, K., McCann, F.E., Davis, D.M., Intercellular transfer and supramolecular organization of human leukocyte antigen C at inhibitory natural killer cell immune synapses (2001) J. Exp. Med., 194, pp. 1507-1517; 
Fassett, M.S., Davis, D.M., Valter, M.M., Cohen, G.B., Strominger, J.L., Signaling at the inhibitory natural killer cell immune synapse regulates lipid raft polarization but not class I MHC clustering (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 14547-14552; 
Faure, M., Barber, D.F., Takahashi, S.M., Jin, T., Long, E.O., Spontaneous clustering and tyrosine phosphorylation of NK cell inhibitory receptor induced by ligand binding (2003) J. Immunol., 170, pp. 6107-6114; 
Standeven, L.J., Carlin, L.M., Borszcz, P., Davis, D.M., Burshtyn, D.N., The actin cytoskeleton controls the efficiency of killer Ig-like receptor accumulation at inhibitory NK cell immune synapses (2004) J. Immunol., 173, pp. 5617-5625; 
Abeyweera, T.P., Merino, E., Huse, M., Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells (2011) J. Cell Biol., 192, pp. 675-690; 
Labadia, M.E., Jeanfavre, D.D., Caviness, G.O., Morelock, M.M., Molecular regulation of the interaction between leukocyte function-associated antigen-1 and soluble ICAM-1 by divalent metal cations (1998) J. Immunol., 161, pp. 836-842; 
Tominaga, Y., Kita, Y., Satoh, A., Asai, S., Kato, K., Ishikawa, K., Horiuchi, T., Takashi, T., Affinity and kinetic analysis of the molecular interaction of ICAM-1 and leukocyte function-associated antigen-1 (1998) Journal of Immunology, 161 (8), pp. 4016-4022; 
Maenaka, K., Van Der Merwe, P.A., Stuart, D.I., Jones, E.Y., Sondermann, P., The human low affinity Fcgamma receptors IIa, IIb, and III bind IgG with fast kinetics and distinct thermodynamic properties (2001) J. Biol. Chem., 276, pp. 44898-44904; 
Dustin, M.L., Ferguson, L.M., Chan, P.Y., Springer, T.A., Golan, D.E., Visualization of CD2 interaction with LFA-3 and determination of the twodimensional dissociation constant for adhesion receptors in a contact area (1996) J. Cell Biol., 132, pp. 465-474; 
Sloan-Lancaster, J., Presley, J., Ellenberg, J., Yamazaki, T., Lippincott-Schwartz, J., Samelson, L.E., ZAP-70 association with T cell receptor zeta (TCRzeta): Fluorescence imaging of dynamic changes upon cellular stimulation (1998) J. Cell Biol., 143, pp. 613-624; 
Von Essen, M., Bonefeld, C.M., Siersma, V., Rasmussen, A.B., Lauritsen, J.P.H., Nielsen, B.L., Geisler, C., Constitutive and ligand-induced TCR degradation (2004) Journal of Immunology, 173 (1), pp. 384-393; 
Groves, J.T., Wulfing, C., Boxer, S.G., Electrical manipulation of glycan-phosphatidyl inositol-tethered proteins in planar supported bilayers (1996) Biophys. J., 71, pp. 2716-2723; 
Krogsgaard, M., Li, Q.J., Sumen, C., Huppa, J.B., Huse, M., Davis, M.M., Agonist/endogenous peptide-MHC heterodimers drive T cell activation and sensitivity (2005) Nature, 434, pp. 238-243; 
Dimasi, N., Sawicki, M.W., Reineck, L.A., Li, Y., Natarajan, K., Margulies, D.H., Mariuzza, R.A., Crystal structure of the Ly49I natural killer cell receptor reveals variability in dimerization mode within the Ly49 family (2002) J. Mol. Biol., 320, pp. 573-585; 
Natarajan, K., Boyd, L.F., Schuck, P., Yokoyama, W.M., Eliat, D., Margulies, D.H., Interaction of the NK cell inhibitory receptor Ly49A with H-2Dd: Identification of a site distinct from the TCR site (1999) Immunity, 11, pp. 591-601; 
O{\textquoteright}Callaghan, C.A., Cerwenka, A., Willcox, B.E., Lanier, L.L., Bjorkman, P.J., Molecular competition for NKG2D: H60 and RAE1 compete unequally for NKG2D with dominance of H60 (2001) Immunity, 15, pp. 201-211; 
Borrego, F., Masilamani, M., Marusina, A.I., Tang, X., Coligan, J.E., The CD94/NKG2 family of receptors: From molecules and cells to clinical relevance (2006) Immunol. Res., 35, pp. 263-278
},
	month = {2011///},
	pages = {760 - 773},
	abstract = {NK cell activation is regulated by a balance between activating and inhibitory signals. To address the question of how these signals are spatially integrated, we created a computer simulation of activating and inhibitory NK cell immunological synapse (NKIS) assembly, implementing either a "quantity-based" inhibition model or a "distance-based" inhibition model. The simulations mimicked the observed molecule distributions in inhibitory and activating NKIS and yielded several new insights. First, the total signal is highly influenced by activating complex dissociation rates but not by adhesion and inhibitory complex dissociation rates. Second, concerted motion of receptors in clusters significantly accelerates NKIS maturation. Third, when the potential of a cis interaction between Ly49 receptors and MHC class I on murine NK cells was added to the model, the integrated signal as a function of receptor and ligand numbers was only slightly increased, at least up to the level of 50\% cis-bound Ly49 receptors reached in the model. Fourth, and perhaps most importantly, the integrated signal behavior obtained when using the distance-based inhibition signal model was closer to the experimentally observed behavior, with an inhibition radius of the order 3-10 molecules. Microscopy to visualize Vav activation in NK cells on micropatterned surfaces of activating and inhibitory strips revealed that Vav is only locally activated where activating receptors are ligated within a single NK cell contact. Taken together, these data are consistent with a model in which inhibitory receptors act locally; that is, that every bound inhibitory receptor acts on activating receptors within a certain radius around it. Copyright {\textcopyright} 2011 by The American Association of Immunologists, Inc.},
	keywords = {animal cell, animal experiment, Animals, antigen recognition, article, cell maturation, cell membrane, Computer simulation, controlled study, dissociation, H-2 Antigens, Histocompatibility Antigens Class I, HLA antigen, HLA C antigen, Humans, immunological synapse, Immunological Synapses, intercellular adhesion molecule 1, Intercellular Adhesion Molecule-1, killer cell immunoglobulin like receptor 2DL1, Killer Cells, Natural, lymphocyte activation, lymphocyte function associated antigen 1, Lymphocyte Function-Associated Antigen-1, major histocompatibility antigen class 1, Membrane Microdomains, Mice, Models, Immunological, mouse, natural killer cell, natural killer cell receptor NKG2D, NK Cell Lectin-Like Receptor Subfamily A, NK Cell Lectin-Like Receptor Subfamily K, nonhuman, priority journal, protein assembly, Receptors, KIR, signal transduction, steady state, Vav protein},
	isbn = {00221767 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960517468\&partnerID=40\&md5=71d9c497ce196d28399f8205f992b389},
	author = {Kaplan, A. and Kotzer, S. and Almeida, C.R. and Kohen, R. and Halpert, G. and Salmon-Divon, M. and K{\"o}hler, K. and H{\"o}glund, P. and Davis, D.M. and Mehr, R.}
}
@article {2816,
	title = {Systemic delivery of bone marrow-derived mesenchymal stromal cells diminishes neuropathology in a mouse model of Krabbe{\textquoteright}s disease},
	journal = {Stem Cells},
	volume = {29},
	year = {2011},
	note = {Cited By (since 1996):4Export Date: 2 October 2013Source: Scopus},
	month = {2011///},
	pages = {1738 - 1751},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80054957115\&partnerID=40\&md5=460f6b3c9735980171039cf772450e6f},
	author = {Miranda, C.O. and Teixeira, C.A. and Liz, M.A. and Sousa, V.F. and Franquinho, F. and Forte, G. and Di Nardo, P. and Pinto-Do-{\'O}, P. and Sousa, M.M.}
}
@article {2281,
	title = {Adhesion of human leukocytes on mixtures of hydroxyl- and methyl-terminated self-assembled monolayers: Effect of blood protein adsorption},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {93},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32480PubMed ID: 19484768Language of Original Document: EnglishCorrespondence Address: Barbosa, J. N.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Porto 4150-180, Portugal; email: judite@ineb.up.ptChemicals/CAS: Blood Proteins; Gold, 7440-57-5; Hydroxyl Radical, 3352-57-6References: Brodbeck, W.G., Nakayama, Y., Matsuda, T., Colton, E., Ziats, N.P., Anderson, J.M., Biomaterial surface chemistry dictates adherent monocyte/macrophage cytokine expression in vitro (2002) Cytokine, 18, pp. 311-319;Kao, W.Y.J., Evaluation of leukocyte adhesion on polyurethanes: The effects of shear stress and blood proteins (2000) Biomaterials, 21, pp. 2295-2303; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Nimeri, G., Ohman, L., Elwing, H., Wettero, J., Bengtsson, T., The influence of plasma proteins and platelets on oxygen radical production and F-actin distribution in neutrophils adhering to polymer surfaces (2002) Biomaterials, 23, pp. 1785-1795; 
Wettero, J., Bengtsson, T., Tengvall, P., C1q-independent activation of neutrophils by immunoglobulin M-coated surfaces (2001) J Biomed Mater Res, 57, pp. 550-558; 
Yung, L.Y.L., Colman, R.W., Cooper, S.L., Neutrophil adhesion on polyurethanes preadsorbed with high molecular weight kininogen (1999) Blood, 94, pp. 2716-2724; 
Eriksson, C., Nygren, H., Polymorphonuclear leukocytes in coagulating whole blood recognize hydrophilic and hydrophobic titanium surfaces by different adhesion receptors and show different patterns of receptor expression (2001) J Lab Clin Med, 137, pp. 296-302; 
Tang, L.P., Eaton, J.W., Mechanism of acute inflammatory response to biomaterials (1994) Cell Mater, 4, pp. 429-436; 
Tang, L.P., Eaton, J.W., Inflammatory responses to biomaterials (1995) Am J Clin Pathol, 103, pp. 466-471; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (1999) J Biomed Mater Res, 49, pp. 435-447; 
Collier, T.O., Anderson, J.M., Protein and surface effects on monocyte and macrophage adhesion, maturation, and survival (2002) J Biomed Mater Res, 60, pp. 487-496; 
Yun, J.K., Defife, K., Colton, E., Stack, S., Azeez, A., Cahalan, L., Verhoeven, M., Anderson, J.M., Human monocytemacrophage adhesion and cytokine production on surface-modified poly(tetrafluoroethylene hexafluoropropylene) polymers with and without protein preadsorption (1995) J Biomed Mater Res, 29, pp. 257-268; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Mrksich, M., Tailored substrates for studies of attached cell culture (1998) Cell Mol Life Sci, 54, pp. 653-662; 
Mrksich, M., Model substrates for studying and controlling cell adhesion (2000) Abstr Pap Am Chem Soc, 219, pp. U572-U572; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolavers (2003) J Biomed Mater Res, 67 A, pp. 158-171. , Part A; 
Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110; 
Tang, L.P., Liu, L., Elwing, H.B., Complement activation and inflammation triggered by model biomaterial surfaces (1998) J Biomed Mater Res, 41, pp. 333-340; 
Bergman, A.J., Zygourakis, K., Migration of lymphocytes on fibronectin-coated surfaces: Temporal evolution of migratory parameters (1999) Biomaterials, 20, pp. 2235-2244; 
Taborelli, M., Eng, L., Descouts, P., Ranieri, J.P., Bellamkonda, R., Aebischer, P., Bovine serum-albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopy (1995) J Biomed Mater Res, 29, pp. 707-714; 
Liu, L., Elwing, H., Complement activation on thiol-modi.ed gold surfaces (1996) J Biomed Mater Res, 30, pp. 535-541; 
Sperling, C., Schweiss, R.B., Streller, U., Werner, C., In vitro hemocompatibility of self-assembled monolayers displaying various functional groups (2005) Biomaterials, 26, pp. 6547-6557; 
Hirata, I., Hioko, Y., Toda, M., Kitazawa, T., Murakami, Y., Kitano, E., Kitamura, H., Iwata, H., Deposition of complement protein C3b on mixed self-assembled monolayers carrying surface hydroxyl and methyl groups studied by surface plasmon resonance (2003) J Biomed Mater Res, 66 A, pp. 669-676. , Part A; 
Kalltorp, M., Oblogina, S., Jacobsson, S., Karlsson, A., Tengvall, P., Thomsen, P., In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces (1999) Biomaterials, 20, pp. 2123-2137; 
Brash, J.L., Tenhove, P., Protein adsorption studies on standard polymeric materials (1993) J Biomater Sci Polym Ed, 4, pp. 591-599; 
Brash, J.L., Tenhove, P., Effect of plasma dilution on adsorption of fibrinogen to solid-surfaces (1984) Thromb Haemost, 51, pp. 326-330; 
Tang, L.P., Ugarova, T.P., Plow, E.F., Eaton, J.W., Molecular determinants of acute inflammatory responses to biomaterials (1996) J Clin Invest, 97, pp. 1329-1334; 
Thomsen, P., Gretzer, C., Macrophage interactions with modified material surfaces (2001) Curr Opin Solid State Mater Sci, 5, pp. 163-176; 
Werthen, M., Sellborn, A., Kalltorp, M., Elwing, H., Thomsen, P., In vitro study of monocyte viability during the initial adhesion to albumin- and fibrinogen-coated surfaces (2001) Biomaterials, 22, pp. 827-832; 
Wettero, J., Bengtsson, T., Tengvall, P., Complement activation on immunoglobulin G-coated hydrophobic surfaces enhances the release of oxygen radicals from neutrophils through an actin-dependent mechanism (2000) J Biomed Mater Res, 51, pp. 742-751; 
Jenney, C.R., Anderson, J.M., Adsorbed IgG: A potent adhesive substrate for human macrophages (2000) J Biomed Mater Res, 50, pp. 281-290; 
Lindblad, M., Lestelius, M., Johansson, A., Tengvall, P., Thomsen, P., Cell and soft tissue interactions with methyl- and hydroxyl-terminated alkane thiols on gold surfaces (1997) Biomaterials, 18, pp. 1059-1068; 
Roach, P., Farrar, D., Perry, C.C., Interpretation of protein adsorption: Surface-induced conformational changes (2005) J Am Chem Soc, 127, pp. 8168-8173; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Adhesion of human leukocytes to biomaterials: An in vitro study using alkanethiolate monolayers with different chemically functionalized surfaces (2003) J Biomed Mater Res, 65 A, pp. 429-434. , Part A; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold (2004) Biomaterials, 25, pp. 2557-2563; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Inflammatory cell recruitment and adhesion to methyl-terminated selfassembled monolayers: Effect of implantation time (2005) Microsc Res Tech, 66, pp. 37-42; 
Barbosa, J.N., Madureira, P., Barbosa, M.A., Aguas, A.P., The attraction of Mac-1(+) phagocytes during acute inflammation by methyl-coated self-assembled monolayers (2005) Biomaterials, 26, pp. 3021-3027
},
	month = {2010///},
	pages = {12 - 19},
	abstract = {The adhesion of human leukocytes to nanostructured surfaces with different chemical properties and the effect of protein adsorption were investigated. Selfassembled monolayers (SAMs) prepared with mixtures of methyl- and hydroxyl-terminated alkanethiols in different percentages on gold were used. The surfaces were preimmersed in distinct protein solutions (human serum albumin, human fibrinogen, and autologous plasma). Adherent leukocytes were analyzed both by light and SEM. SAMs submitted to pre-immersion in plasma presented higher numbers of adherent leukocytes in the pure OH-terminated SAM, whereas methyl-terminated surfaces accounted for the lowest number of adherent cells. We observed a general increase in the number of adherent human leukocytes as the percentage of OH groups on the surface of the SAMs increased for all the pre-immersion conditions investigated. The number of adherent human leukocytes is highly influenced by the pre-immersion conditions used, and this observation is particularly relevant in the case of the methyl-terminated SAMs. The results obtained demonstrate that surface chemistry has a major influence in leukocyte adhesion to biomaterials, and that pre-immersion in protein solutions has a determinant effect in leukocyte adhesion. {\textcopyright} 2009 Wiley Periodicals, Inc.},
	keywords = {Adherent cells, Adhesion, Adsorption, Alkanethiols, article, Biological materials, biomaterial, Blood protein adsorption, Blood Proteins, cell adhesion, Cell Count, Chemical properties, gold, human, human cell, Human leukocytes, Human serum albumins, Humans, hydroxyl group, Hydroxyl Radical, Hydroxyl-terminated, immersion, Inflammatory response, leukocyte adherence, Leukocyte adhesion, Leukocytes, Nanostructured materials, Nanostructured surface, Nanostructured surfaces, OH group, Protein adsorption, protein blood level, Protein solution, Proteins, Sams, Scanning electron microscopy, Self assembled monolayers, Self-assembled monolayers, SEM, Surface chemistry, Surface Properties},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77649225392\&partnerID=40\&md5=0b35403e1b98581c557435b6cf7a8286},
	author = {Barbosa, J.N. and Martins, M.C.L. and Freitas, S.C. and Gon{\c c}alves, I.C. and {\'A}guas, A.P. and Barbosa, M.A.}
}
@article {2268,
	title = {Bioactivity of immobilized EGF on self-assembled monolayers: Optimization of the immobilization process},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {94},
	year = {2010},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32723PubMed ID: 20198690Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R.; INEB, Biomaterials Division, NEWTherapies Group, R. Campo Alegre, 823, Porto 4150-180, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: epidermal growth factor, 62229-50-9; gold, 7440-57-5; Biocompatible Materials; Epidermal Growth Factor, 62229-50-9; Gold, 7440-57-5; Imidazoles; N,N-carbonyldiimidazole, 530-62-1; Polyethylene Glycols; Receptor, Epidermal Growth Factor, 2.7.10.1; Sulfhydryl CompoundsReferences: Singh, A., Harris, R.C., Autocrine, paracrine and juxtacrine signaling by EGFR ligands (2005) Cell Signal, 17, pp. 1183-1193;Luca, A., Carotenuto, A., Rachiglio, A., Gallo, M., Maiello, M.R., Aldinucci, D.L., Pinto, A., Normanno, N., The role of the EGFR signaling in tumor microenvironment (2008) J Cell Physiol, 214, pp. 559-567; 
Stenflo, J., Stenberg, Y., Muranyi, A., Calcium-binding EGF-like modules in coagulation proteinases: Function of the calcium ion in module interactions (2000) Biochim Biophys Acta, 1477, pp. 51-63; 
Leong, K., Karsan, A., Recent insights in the role of notch signaling in tumorigenesis (2006) Blood, 107, pp. 2223-2233; 
Sakamoto, K., Chao, W.S., Katsube, K.-I., Yamaguchi, A., Distinct roles of EGF repeats for the Notch signaling system (2005) Experimental Cell Research, 302 (2), pp. 281-291. , DOI 10.1016/j.yexcr.2004.09.016, PII S0014482704005658; 
Savage, C., Hash, J.H., Cohen, S., Epidermal growth factor (1973) J Biol Chem, 248, pp. 7669-7672; 
Bell, G., Fong, N.M., Stempien, M.M., Wormsted, M.A., Caput, D., Ku, L., Urdea, M.S., Sanchez-Pescador, R., Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization (1986) Nucleic Acids Res, 14, pp. 8427-8446; 
Carpenter, G., Cohen, S., Epidermal growth factor (1990) J Biol Chem, 265, pp. 7709-7712; 
Schlessinger, J., Allosteric regulation of epidermal growth factor receptor kinase (1986) J Cell Biol, 103, pp. 2067-2072; 
Mitamura, T., Higashiyama, S., Taniguchi, N., Klagsbrun, M., Mekada, E., Diphtheria toxin binds to the epidermal growth factor (EGF)-like domain of human heparin-binding EGF-like growth factor/diphtheria toxin receptor and inhibits specifically its mitogenic activity (1995) J Biol Chem, 270, pp. 1015-1019; 
Wong, R.W.C., Guillaud, L., The role of epidermal growth factor and its receptors in mammalian CNS (2004) Cytokine Growth Factor Rev, 15, pp. 147-156; 
Mrksich, M., What can surface chemistry do for cell biology? (2002) Curr Opion Chem Biol, 6, pp. 794-797; 
Tohda, S.K.H., Murakami, N., Sakano, S., Nara, N., Diverse effects of the notch ligands Jagged1 and Delta1 on the growth and differentiation of primary acute myeloblastic leukemia cells (2005) Exp Hematol, 33, pp. 558-563; 
France, R.M., Short, R.D., Dawson, R.A., MacNeil, S., Attachment of human keratinocytes to plasma co-polymers of acrylic acid/octa-1,7-diene and allyl amine/octa-1,7-diene (1998) Journal of Materials Chemistry, 8 (1), pp. 37-42; 
Love, J., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Selfassembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1169; 
Saha, K.P.J., Schaffer, D.V., Healy, K.E., Designing synthetic materials to control stem cell phenotype (2007) Curr Opin Chem Biol, 11, pp. 381-387; 
Barbosa, J.B.M., {\'A}guas, A.P., Adhesion of human leukocytes to biomaterials: An in vitro study using alkanethiolate monolayers with different chemically functionalized surfaces (2003) J Biomed Mater Res A, 65, pp. 429-434; 
Rodrigues, S.N.G.I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Barrias, C., Martins, M.C., Almeida-Porada, G., Barbosa, M.A., Granja, P.L., The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers (2009) Biomaterials, 30, pp. 307-316; 
Curran, J.M.C.R., Hunt, J.A., The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate (2006) Biomaterials, 27, pp. 4783-4793; 
Lee, M., Brass, D.A., Morris, R., Composto, R.J., Ducheyne, P., The effect of non-specific interactions on cellular adhesion using model surfaces (2005) Biomaterials, 26, pp. 1721-1730; 
Martins, M.C.L.C.S., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res A, 88, pp. 162-173; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Martins, M.C.L.R.B., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl- Terminated self-assebled monolyers (2003) J Biomed Mater Res, 67, pp. 158-171; 
Barrias, C.C.M.M., Bidarra, S., S{\'a}miranda, M.C., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: Effect of the surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, , Amersham Life Science. Amersham Place, Little Chalfont, Bucks: Amersham International plc; 
Zhang, J., Matthews, J.M., Ward, L.D., Simpson, R.J., Disruption of the disulfide bonds of recombinant murine interleukin-6 induces formation of a partially unfolded state (1997) Biochemistry, 36, pp. 2380-2389; 
Solomons, T., (1996) Organic Chemistry, , New York: Wiley; 
Lukkari, J., Meretoja, M., Kartio, I., Laajalehto, K., Rajamaki, M., Lindstrom, M., Kankare, J., Organic thiosulfates (bunte salts): Novel surface-active sulfur compounds for the preparation of selfassembled monolayers on gold (1999) Langmuir, 15, pp. 3529-3537; 
Castner, D.G., Hinds, K., Grainger, D.W., X-ray photoelectron spectroscopy sulfur 2p study of organic thiol and bisulfide binding interactions with gold surfaces (1996) Langmuir, 12 (21), pp. 5083-5086; 
Molina, R., Espin{\'o}s, J.P., Yubero, F., Erra, P., Gonz{\'a}lez-Elipe, A.R., XPS analysis of down stream plasma treated wool: Influence of the nature of the gas on the surface modification of wool (2005) Appl Surf Sci, 252, pp. 1417-1429; 
Flynn, N., Tran, T.N.T., Cima, M.J., Langer, R., Long-term stability of self-assembled monolayers in biological media (2003) Langmuir, 19, pp. 10909-10915; 
Willey, T., Vance, A.L., Van Buuren, T., Bostedt, C., Terminello, L.J., Fadley, C.S., Rapid degradation of alkanethiol-based self-assembled monolayers on gold in ambient laboratory conditions (2005) Surf Sci, 576, pp. 188-196; 
Lage, A., Crombet, T., Gonz{\'a}lez, G., Targeting epidermal growth factor receptor signaling: Early results and future trends in oncology (2003) Trends Mol Med, 35, pp. 327-336; 
Lee, H., Hu, M., Reilly, R.M., Allen, C., Apoptotic epidermal growth factor (EGF)- Conjugated block copolymer micelles as a nanotechnology platform for targeted combination therapy (2007) Mol Pharm, 4, pp. 769-781; 
Nakaji-Hirabayashi, T., Kato, K., Arima, Y., Iwata, H., Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells (2007) Biomaterials, 28, pp. 3517-3529; 
Stefonek, T., Masters, K.S., Immobilized gradients of epidermal growth factor promote accelerated and directed keratinocyte migration (2007) Wound Repair Regen, 15, pp. 847-855; 
Ito, Y., Li, J.S., Takahashi, T., Imanishi, Y., Okabayashi, Y., Kido, Y., Kasuga, M., Enhancement of the mitogenic effect by artificial juxtacrine stimulation using immobilized EGF (1997) J Biochem, 121, pp. 514-520; 
Wang, Q., Bohn, P.W., Surface composition gradients of immobilized cell signaling molecules: Epidermal growth factor on gold (2006) Thin Solid Films, 513, pp. 338-346; 
Karakecili, A., Satriano, C., Gumusderelioglu, M., Marletta, G., Enhancement of fibroblastic proliferation on chitosan surfaces by immobilized epidermal growth factor (2008) Acta Biomater, 4, pp. 989-996; 
Hodneland, C., Lee, Y.S., Min, D.H., Mrksich, M., Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands (2002) PNAS, 99, pp. 5048-5052; 
Liu, L., Chen, S., Giachelli, C.M., Ratner, B.D., Jiang, S., Controlling osteopontin orientation on surfaces to modulate endothelial cell adhesion (2005) J Biomed Mater Res A, 74, pp. 23-31; 
Kim, H., Noh, J., Hara, M., Lee, H., Characterization of mixed selfassembled monolayers for immobilization of streptavidin using chemical force microscopy (2008) Ultramicroscopy, 108, pp. 1140-1143; 
Delamarche, E., Sundarababu, G., Biebuyck, H., Michel, B., Gerber, Ch., Sigrist, H., Wolf, H., Mathieu, H.J., Immobilization of antibodies on a photoactive self-assembled monolayer on gold (1996) Langmuir, 12 (8), pp. 1997-2006; 
Gon{\c c}alves, R., Martins, M.C.L., Almeida-Porada, G., Barbosa, M.A., Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers (2009) Biomaterials, 30, pp. 6879-6887; 
Mrksich, M., Whitesides, G.M., Using Self-Assembled Monolayers That Present Oligo(ethylene glycol) Groups to Control the Interactions of Proteins with Surfaces (1997) ACS Symposium Series, 680, pp. 361-373; 
Chen, G., Ito, Y., Gradient micropattern immobilization of EGF to investigate the effect of artificil juxtacrine stimulation (2001) Biomaterials, 22, pp. 2453-2457; 
Allewood, D., Nielsen, K., Alewwod, P.F., Craik, D.J., Andrews, P., Nerrie, M., White, S., Nice, E.C., The role of disulfide bonds in the structure and function of murine epidermal growth factor (mEGF) (2005) Growth Factors, 23, pp. 97-110; 
Morton, R., Mangroo, D., Gerber, G.E., A novel method of complete activation by carbonyldiimidazole: Application to esther synthesis (1988) Can J Chem, 66, pp. 1701-1705; 
Stollner, D., Scheller, F.W., Warsinke, A., Activation of cellulose membranes with 1,1-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors (2002) Anal Biochem, 304, pp. 157-165; 
Crowley, S.C., Chan, K.C., Walters, R.R., Optimization of protein immobilization on 1,1'-carbonyldiimidazole- activated diol-bonded silica (1986) J Chromatogr A, 359, pp. 359-368; 
Mateus, A., Seruca, R., Machado, J.C., Keller, G., Oliveira, M.J., Suriano, G., Luber, B., EGFR regulates RhoA-GTP dependent cell motility in E-cadherin mutant cells (2007) Hum Mol Genet, 16, pp. 1639-1647; 
Garcia, R., Franklin, R.A., Mccubrey, J.A., EGF induces cell motility and multi-drug resistance gene expression in breast cancer cells (2006) Cell Cycle, 5, pp. 2820-2826
},
	month = {2010///},
	pages = {576 - 585},
	abstract = {Last trends in Biomaterials focus the mimic of cellular environments capable to control cellular responses. Epidermal growth factor (EGF) is a pleiotropic cytokine known to regulate cell proliferation, differentiation, and death. This study aims to optimize the immobilization of EGF on 11-mercapto-1-undecyl-tetra(ethylene)glycol (EG4) - self-assembled monolayers (SAMs) and to establish a new model surface to study EGF-mediated signaling. Gold substrates were modified with a monolayer of EG4 and N,N'- carbonyldiimidazole (CDI) was used to activate hydroxyl terminated groups of EG4-SAMs. EGF was then immobilized on activated EG4-SAMs at pH 7.4, 4{\textdegree}C, and 100 rpm. Different immobilization reaction times were tested as well as different CDI concentrations to optimize the reaction conditions and obtain a range of immobilized EGF concentrations on the surfaces. Surface characterization of EGF-SAMs was performed using radiolabeling, water contact angle measurements, X-ray photoelectron spectroscopy, and ELISA. Phosphorylation of EGFR on BT-20 breast cancer cell line by EGF-SAMs was tested by immunostaining. EGF was successfully immobilized on EG4-SAMs, at 4{\textdegree}C and pH 7.4 in a range of concentrations from 3.6 {\textpm} 0.8 to 17.6 {\textpm} 1.5 ng/cm                        2. The concentration of EGF increases with immobilization time and with the CDI concentration reaching the maximum for surfaces activated with 30 mg/mL of CDI after 48 h. The bioactivity of EGF-SAMs was confirmed by immunostaining of phospho-EGFR of BT-20 cells. This study described EGF immobilization on EG4-SAMs at different concentrations, which could be important surface models to study specific protein interactions at the molecular level evolving EGF-family of proteins. {\textcopyright} 2010 Wiley Periodicals, Inc. J.
},
	keywords = {Adsorption, Angle measurement, article, Biocompatible Materials, Biological materials, Breast cancer cells, cancer cell culture, Carbonyldiimidazole, Cell culture, Cell immobilization, Cell Line, Tumor, cell proliferation, Cellular environment, Cellular response, Concentration (process), Concentration of, Contact angle, controlled study, Cytokines, EGF, Electron energy levels, enzyme linked immunosorbent assay, Enzyme-Linked Immunosorbent Assay, epidermal growth factor, Epidermal growth factors, Ethylene, Glycols, gold, Gold substrates, human, human cell, Humans, Hydroxyl-terminated, Imidazoles, Immobilization process, Immobilization reaction, immunofluorescence, immunohistochemistry, Immunostaining, Molecular levels, Nanostructured materials, Nanostructured surface, Nanostructured surfaces, New model, Optimization, Phosphorylation, Photoelectron spectroscopy, Polyethylene Glycols, Protein adsorption, protein immobilization, protein interaction, protein phosphorylation, Proteins, Radiolabeling, Radioligand Assay, Reaction conditions, reaction optimization, Receptor, Epidermal Growth Factor, Sams, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, Sulfhydryl Compounds, Surface characterization, Surface functionalization, Surface models, Surface Properties, Water contact angle measurement, X ray photoelectron spectroscopy},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953815323\&partnerID=40\&md5=7b54d717bbba475933dd86cc62659acc},
	author = {Gon{\c c}alves, R. and Martins, M.C.L. and Oliveira, M.J. and Almeida-Porada, G. and Barbosa, M.A.}
}
@article {2267,
	title = {Cell nuclei and cytoplasm joint segmentation using the sliding band filter},
	journal = {IEEE Transactions on Medical ImagingIEEE Trans. Med. Imaging},
	volume = {29},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusArt. No.: 5477157CODEN: ITMIDdoi: 10.1109/TMI.2010.2048253Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; Instituto de Engenharia Biom{\'e}dica (INEB), 4200-465 Porto, Portugal; email: pedro.quelhas@gmail.comChemicals/CAS: DNA, 9007-49-2; Actins; DNA, 9007-49-2References: Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Understand., 77 (3), pp. 317-370;Clocksin, W., Automatic segmentation of overlapping nuclei with high background variation using robust estimation and flexible contour models (2003) Proc. 12th Int. Conf. Image Anal. Process., pp. 682-687; 
Cloppet, F., Boucher, A., Segmentation of overlapping/aggregating nuclei cells in biological images (2008) Proc. 19th Int. Conf. Pattern Recognit. (ICPR 2008), pp. 1-4; 
Danek, O., Matula, P., De Solorzano, C.O., Munoz-Barrutia, A., Maska, M., Kozubek, M., Segmentation of touching cell nuclei using a two-stage graph cut model (2009) LNCS, 5575, pp. 410-419. , Proc. SCIA; 
Fok, Y., Chan, J., Chin, R., Automated analysis of nerve-cell images using active contour models (1996) IEEE Trans. Med. Imag., 15 (3), pp. 353-368. , Jun; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3-D fluorescence microscopy image sequences (2008) Workshop Bio-Image Inf.: Biol. Imag., Comput. Vis. Data Mining, pp. 17-18. , Santa Barbara, CA; 
Hill, A., Lapan, P., Li, Y., Haney, S., Impact of image segmentation on high-content screening data quality for sk-br-3 cells (2007) BMC Bioin-formatics, 8 (340), pp. 1-13; 
Hu, M., Ping, X., Ding, Y., A new active contour model and its application on cell segmentation (2004) 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV), 2, pp. 1104-1107. , TuA7.09 - P0673, 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV); 
Jones, T.R., Carpenter, A., Golland, P., Voronoi-based segmentation of cells on image manifolds (2005) ICCV Workshop Comput. Vis. Biomed. Image Appl. (CVBIA), pp. 535-543; 
Klemencic, A., Kovacic, S., Pernus, F., Automated segmentation of muscle fiber images using active contour models (1998) Cytometry, 32, pp. 317-326; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. Image Process., 8 (8), pp. 1029-1038. , Aug; 
Lehmussola, A., Ruusuvuori, P., Selinummi, J., Huttunen, H., Yli-Harja, O., Computational framework for simulating fluorescence microscope images with cell populations (2007) IEEE Trans. Med. Imag., 26 (7), pp. 1010-1016. , Jul; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. Pattern Anal. Mach. Intell., 13 (6), pp. 583-598. , Jun; 
Marcuzzo, M., Guichard, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Proceedings of IbPRIA. New York: Springer; 
Marcuzzo, M., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images (2009) LNCS, 5627, pp. 824-831. , Proceedings of ICIAR. New York: Springer; 
Moore, P., Molloy, D., A survey of computer-based deformable models (2007) Int. Mach. Vis. Image Process. Conf. (IMVIP), pp. 55-66; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Tran. Syst., Man, Cybern., 9 (1), pp. 62-66. , Jan; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A., Detection of lung nodule candidates in chest radiographs (2007) LNCS, 4478, pp. 170-177; 
Pereira, C.S., Mendon{\c c}a, A.M., Campilho, A., Evaluation of contrast enhancement filters for lung nodule detection Proceedings of ICIAR. New York: Springer, 4633, pp. 878-888; 
Quelhas, P., Marcuzzo, M., Oliveira, M.J., Mendon{\c c}a, A.M., Campilho, A., Cancer cell detection and invasion depth estimation in brightfield images (2009) Proc. 20th Br. Mach. Vis. Conf. (BMVC), , London, U.K; 
Ruusuvuori, P., Lehmussola, A., Selinummi, J., Rajala, T., Huttunen, H., Yli-Harja, O., Benchmark set of synthetic images for validating cell image analysis algorithms (2008) Proc. 16th Eur. Signal Process. Conf., , Lausanne, Switzerland; 
Solorzano, C., Malladi, R., Lelievre, S., Lockett, S., Segmentation of nuclei and cells using membrane related protein markers (2001) J. Microscopy, 201, pp. 404-415; 
Tek, F.B., Dempster, A.G., Kale, I., (2006) Mathematical Morphology:40 Years on, 30, pp. 441-454. , New York: Springer; 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proc. Int. Conf. Image Anal. Process., pp. 757-761; 
Xiong, G., Zhou, X., Ji, L., Bradley, P., Perrimon, N., Wong, S., Segmentation of drosophila RNAI fluorescence images using level sets (2006) Proc. IEEE Int. Conf. Image Process., pp. 73-76; 
Xiong, G., Zhou, X., Ji, L., Automated segmentation of drosophila RNAi fluorescence cellular images using deformable models (2006) IEEE Trans. Circ. Syst. i, 53 (11), pp. 2415-2424. , Nov; 
Yan, P., Zhou, X., Shah, M., Wong, S.T.C., Automatic segmentation of high-throughput RNAi fluorescent cellular images (2008) IEEE Trans. Inf. Technol. Biomed., 12 (1), pp. 109-117. , Jan; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. Circuits Syst., 53 (11), pp. 2405-2414. , Nov
},
	month = {2010///},
	pages = {1463 - 1473},
	abstract = {Microscopy cell image analysis is a fundamental tool for biological research. In particular, multivariate fluorescence microscopy is used to observe different aspects of cells in cultures. It is still common practice to perform analysis tasks by visual inspection of individual cells which is time consuming, exhausting and prone to induce subjective bias. This makes automatic cell image analysis essential for large scale, objective studies of cell cultures. Traditionally the task of automatic cell analysis is approached through the use of image segmentation methods for extraction of cells{\textquoteright} locations and shapes. Image segmentation, although fundamental, is neither an easy task in computer vision nor is it robust to image quality changes. This makes image segmentation for cell detection semi-automated requiring frequent tuning of parameters. We introduce a new approach for cell detection and shape estimation in multivariate images based on the sliding band filter (SBF). This filter{\textquoteright}s design makes it adequate to detect overall convex shapes and as such it performs well for cell detection. Furthermore, the parameters involved are intuitive as they are directly related to the expected cell size. Using the SBF filter we detect cells{\textquoteright} nucleus and cytoplasm location and shapes. Based on the assumption that each cell has the same approximate shape center in both nuclei and cytoplasm fluorescence channels, we guide cytoplasm shape estimation by the nuclear detections improving performance and reducing errors. Then we validate cell detection by gathering evidence from nuclei and cytoplasm channels. Additionally, we include overlap correction and shape regularization steps which further improve the estimated cell shapes. The approach is evaluated using two datasets with different types of data: a 20 images benchmark set of simulated cell culture images, containing 1000 simulated cells; a 16 images Drosophila melanogaster Kc167 dataset containing 1255 cells, stained for DNA and actin. Both image datasets present a difficult problem due to the high variability of cell shapes and frequent cluster overlap between cells. On the Drosophila dataset our approach achieved a precision/recall of 95\%/69\% and 82\%/90\% for nuclei and cytoplasm detection respectively and an overall accuracy of 76\%. {\textcopyright} 2010 IEEE.},
	keywords = {actin, Actins, animal, Animals, article, Band filters, Biological research, cell aggregation, Cell analysis, Cell culture, Cell detection, Cell images, cell nucleus, Cell segmentation, Cell Shape, Cell shapes, Cell size, Cells, chemistry, Computer vision, Convergence filters, Convex shapes, cytoplasm, Data sets, Databases, Factual, Digital image storage, DNA, Drosophila melanogaster, factual database, Fluorescence, Fluorescence microscopy, Fundamental tools, High variability, Image analysis, Image datasets, image processing, image processing in biology, Image Processing, Computer-Assisted, Image quality, Image segmentation, Imaging systems, Improving performance, Individual cells, Joint segmentation, Location, methodology, Microscopy, Fluorescence, Multivariate analysis, multivariate image processing, New approaches, Nuclear detection, Overlap correction, reproducibility, Reproducibility of Results, Semi-automated, Shape estimation, Shape regularization, ultrastructure, Visual communication, Visual inspection},
	isbn = {02780062 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955339493\&partnerID=40\&md5=f8cea406f7b2fcf901dc34fa940522b1},
	author = {Quelhas, P. and Marcuzzo, M. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2270,
	title = {Ceramics for dental restorations - an introduction},
	journal = {Una perspectiva de las cer{\'a}micas para restauraciones dentalesDYNADYNA},
	volume = {77},
	year = {2010},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: English; SpanishCorrespondence Address: Santander, S. A.; Universidad Pontificia Bolivariana and Universidad Cooperativa de Colombia, Medell{\'\i}n, Colombia; email: santiago.arango75@gmail.comReferences: Rosenblum, M.A., Schulman, A., A review of allceramic restorations (1997) J Am Dent Assoc, 128 (3), pp. 297-307;Rizkalla, A.S., Jones, D.W., Mechanical properties of commercial high strength ceramic core materials (2004) Dent Mater, 20 (2), pp. 207-212; 
Rizkalla, A.S., Jones, D.W., Indentation fracture toughness and dynamic elastic moduli for commercial feldspathic dental porcelain materials (2004) Dent Mater, 20 (2), pp. 198-206; 
Jones, D.W., Development of dental ceramics. An historical perspective (1985) Dent Clin North Am, 29 (4), pp. 621-644; 
Kelly, J.R., Nishimura, I., Campbell, S.D., Ceramics in dentistry: Historical roots and current perspectives (1996) J Prosthet Dent, 75 (1), pp. 18-32; 
Stejskal, V.D., Metalspecific lymphocytes: Biomarkers of sensitivity in man (1999) Neuro Endocrinol Lett, 20 (5), pp. 289-298; 
Arvidson, K., Wroblewski, R., Migration of metallic ions from screwposts into dentin and surrounding tissues (1978) Scand J Dent Res, 86 (3), pp. 200-205; 
Venclikova, Z., Metallic pigmentation of human teeth and gingiva: Morphological and immunological aspects (2007) Dent Mater J, 26 (1), pp. 96-104; 
Bumgardner, J.D., Lucas, L.C., Cellular response to metallic ions released from nickelchromium dental alloys (1995) J Dent Res, 74 (8), pp. 15-217; 
Mehulic, K., The release of metal ions in the gingival fluid of prosthodontic patients (2005) Acta Stomatol Croat, 39 (1), pp. 47-51; 
Mart{\'\i}nez, F., Cer{\'a}micas dentales: Clasificaci{\'o}n y criterios de selecci{\'o}n (2007) RCOE, 12 (4), pp. 253-263; 
Weinstein, M., Weinstein, L., Weinstein, A., (1962) Porcelain Coveredmetal Reinforced Teeth; 
Anusavice, K.J., Degradability of dental ceramics (1992) Adv Dent Res, 6, p. 829; 
Knorpp, E.A., (1991) Control Arrangement for Dental Furnaces, Especially Microprocessorcontrolled Preheating Furnaces; 
Anusavice, K.J., (2004) Phillips Ciencia De Los Materiales Dentales, , Und{\'e}cima ed: Elsevier Espa{\~n}a; 
Meyer, J.M., O{\textquoteright}Brien, W.J., Yu, C.U., Sintering of dental porcelain enamels (1976) J Dent Res, 55 (4), pp. 696-699; 
Denry, I.L., Recent advances in ceramics for dentistry (1996) Crit Rev Oral Biol Med, 7 (2), pp. 134-143; 
Campbell, S.D., Dimensional and formation analysis of a restorative ceramic and how it works (1995) J Prosthet Dent, 74 (4), pp. 332-340; 
Conrad, H.J., Seong, W.J., Pesun, I.J., Current ceramic materials and systems with clinical recommendations: A systematic review (2007) J Prosthet Dent, 98 (5), pp. 389-404; 
Tinschert, J., Structural reliability of alumina, feldspar, leucite, micaand zirconiabased ceramics (2000) J Dent, 28 (7), pp. 529-535; 
Rahaman, M.N., (2003) Ceramic Processing and Sintering, , materials engineering, Second ed: CRC Press; 
Anusavice, K.J., Hojjatie, B., Effect of thermal tempering on strength and crack propagation behavior of feldspathic porcelains (1991) J Dent Res, 70 (6), pp. 1009-1013; 
Anusavice, K.J., Shen, C., Lee, R.B., Strengthening of feldspathic porcelain by ion exchange and tempering (1992) J Dent Res, 71 (5), pp. 113-148; 
Dunn, B., Levy, M.N., Reisbick, M.H., Improving the fracture resistance of dental ceramic (1977) J Dent Res, 56 (10), pp. 1209-1213; 
Rosa, V., Effect of ion exchange on strength and slow crack growth of a dental porcelain (2009) Dent Mater, 25 (6), pp. 736-743; 
Seha, M.M., Effect of TufCoat on Feldspathic porcelain materials (2005) J Oral Rehabil, 32 (1), pp. 39-45; 
Anusavice, K.J., Gray, A., Shen, C., Influence of initial flaw size on crack growth in airtempered porcelain (1991) J Dent Res, 70 (2), pp. 131-136; 
Asaoka, K., Kuwayama, N., Tesk, J.A., Influence of tempering method on residual stress in dental porcelain (1992) J Dent Res, 71 (9), pp. 1623-1627; 
Dehoff, P.H., Anusavice, K.J., Vontivillu, S.B., Analysis of tempering stresses in metalceramic disks (1996) J Dent Res, 75 (2), pp. 743-751; 
Cesar, P.F., Correlation between fracture toughness and leucite content in dental porcelains (2005) J Dent, 33, pp. 721-729; 
Bertolotti, R.L., Calculation of interfacial stress in porcelainfusedtometal systems (1980) J Dent Res, 59 (11), pp. 1972-1977; 
Nielsen, J.P., Tuccillo, J.J., Calculation of interfacial stress in dental porcelain bonded to gold alloy substrate (1972) J Dent Res, 51 (4), pp. 104-137; 
Fairhurst, C.W., Porcelainmetal thermal compatibility (1981) J Dent Res, 60 (4), pp. 815-819; 
Vasquez, V., Mechanical and thermal cycling effects on the flexural strength of glass ceramics fused to titanium (2008) Dent Mater J, 27 (1), pp. 7-15; 
Sheu, T., Mechanical properties and thermal expansion behaviour in leucite containing materials (1994) J Mater Sci, 29, pp. 125-128; 
Denry, I.L., Effect of cubic leucite stabilization on the flexural strength of feldspathic dental porcelain (1996) J Dent Res, 75 (12), pp. 1928-1935; 
Pinto, M.M., Influence of pH on slow crack growth of dental porcelains (2008) Dent Mater, 24 (6), pp. 814-823; 
McLean, J.W., Evolution of dental ceramics in the twentieth century (2001) J Prosthet Dent, 85 (1), pp. 61-66; 
Sherrill, C.A., O{\textquoteright}Brien, W.J., Transverse strength of aluminous and feldspathic porcelain (1974) J Dent Res, 53, pp. 683-690; 
Alshehri, S., Relative fracture toughness and hardness of dental ceramics (2002) Saudi Dent J, 14 (2), pp. 67-72; 
Chaiyabutr, Y., Giordano, R., Pober, R., The effect of different powder particle size on mechanical properties of sintered alumina, resinand glassinfused alumina (2009) J Biomed Mater Res B Appl Biomater, 88 (2), pp. 502-508; 
Alvarezfern{\'a}ndez, M.A., Caracter{\'\i}sticas generales y propiedades de las cer{\'a}micas sin metal (2003) RCOE, 8 (5), pp. 525-546; 
Sorensen, J.A., InCeram fixed partial dentures: Threeyear clinical trial results (1998) J Calif Dent Assoc, 26 (3), pp. 207-214; 
Tyszblat, M., (1988) Process for the Preparation of a Dental Prosthesis By Slight Solid Phase Fritting of a Metal Oxide Based Infrastructure; 
Andersson, M., Oden, A., A new allceramic crown. A densesintered, highpurity alumina coping with porcelain (1993) Acta Odontol Scand, 51 (1), pp. 59-64; 
Andersson, M., Clinical results with titanium crowns fabricated with machine duplication and spark erosion (1989) Acta Odontol Scand, 47 (5), pp. 279-286; 
Oilo, G., Flexural strength and internal defects of some dental porcelains (1988) Acta Odontol Scand, 46 (5), pp. 313-322; 
Pel{\'a}ezvargas, A., Microhardness obtained with two ceramic paste preparation methods (2007) J Dent Res, 87 (Spec Iss B), p. 0294; 
Drummond, J.L., Mechanical property evaluation of pressable restorative ceramics (2000) Dent Mater, 16 (3), pp. 226-233; 
Gonzaga, C.C., Mechanical properties and porosity of dental glassceramics hotpressed at different temperatures (2008) Mat Res, 11 (3), pp. 301-306; 
Ardlin, B.I., Transformationtoughened zirconia for dental inlays, crowns and bridges: Chemical stability and effect of lowtemperature aging on flexural strength and surface structure (2002) Dent Mater, 18 (8), pp. 590-595; 
Vleugels, J., Yuan, Z.X., van der Biest, O., Mechanical properties of Y2O3/Al2O3coated YTZP ceramics (2002) J Eur Ceram Soc, 22, pp. 873-881; 
Craig, R.G., Peyton, F.A., The microharness of enamel and dentin (1958) J Dent Res, 37, pp. 661-668; 
Hassan, R., Caputo, A.A., Bunshah, R.F., Fracture toughness of human enamel (1981) J Dent Res, 60 (4), pp. 820-827; 
Rasmussen, S.T., Patchin, R.E., Fracture properties of human enamel and dentin in an aqueous environment (1984) J Dent Res, 63 (12), pp. 1362-1368; 
Fuentes, V., Microhardness of superficial and deep sound human dentin (2003) J Biomed Mater Res A, 66 (4), pp. 850-853; 
el Mowafy, O.M., Watts, D.C., Fracture toughness of human dentin (1986) J Dent Res, 65 (5), pp. 677-681; 
(1982) Glassceramic Dental Products; 
(1973) Tetrasilicic Mica Glassceramic Method; 
(1956) Method of Making Ceramics and Products Thereof; 
Denry, I.L., Rosenstiel, S.F., Flexural strength and fracture toughness of Dicor glassceramic after embedment modification (1993) J Dent Res, 72 (3), pp. 572-576; 
Thompson, J.Y., Fracture surface characterization of clinically failed allceramic crowns (1994) J Dent Res, 73 (12), pp. 1824-1832; 
Elmowafy, O., Brochu, J.F., Longevity and clinical performance of IPSEmpress ceramic restorationsa literature review (2002) J Can Dent Assoc, 68 (4), pp. 233-237; 
Probster, L., In vitro evaluation of a glassceramic restorative material (1997) J Oral Rehabil, 24 (9), pp. 636-645; 
Sorensen, J.A., IPS Empress crown system: Threeyear clinical trial results (1998) J Calif Dent Assoc, 26 (2), pp. 130-136; 
(1990) Dispersion Strengthened Composite; 
Fischer, H., Marx, R., Improvement of strength parameters of a leucitereinforced glass ceramic by dualion exchange (2001) J Dent Res, 80 (1), pp. 336-339; 
Mansour, Y.F., Clinical performance of IPSEmpress 2 ceramic crowns inserted by general dental practitioners (2008) J Contemp Dent Pract, 9 (4), pp. 9-16; 
Brodkin, D., Panzera, C., Panzera, P., (2002) Pressable Lithium Disilicate Glass Ceramics; 
Garvie, R.C., Hannink, R.H., Pascoe, R.T., Ceramic steel? (1975) Nature, 258 (5537), pp. 703-704; 
Piconi, C., Maccauro, G., Zirconia as a ceramic biomaterial (1999) Biomaterials, 20 (1), pp. 1-25; 
Guazzato, M., Strength, fracture toughness and microstructure of a selection of allceramic materials (2004) Zirconiabased Dental Ceramics, Dent Mater, 20 (5), pp. 449-456. , Part II; 
Pilathadka, S., Vahalova, D., Vosahlo, T., The Zirconia: A new dental ceramic material (2007) An Overview, Prague Med Rep, 108 (1), pp. 5-12; 
Deville, S., Lowtemperature ageing of zirconiatoughened alumina ceramics and its implication in biomedical implants (2003) J Eur Ceram Soc, 23, pp. 2975-2982; 
Zivkobabic, J., Carek, A., Jakovac, M., Zirconium oxide ceramics in prosthodontics (2005) Acta Stomatol Croat, 38 (1), pp. 25-28; 
Piconi, C., YTZP ceramics for artificial joint replacements (1998) Biomaterials, 19 (16), pp. 1489-1494; 
Chemical synthesis of ceramic materials (1997) J Mater Chem, 7 (8), pp. 1297-1305
},
	month = {2010///},
	pages = {26 - 36},
	abstract = {Dental ceramics are the preferred materials for oral restoration due to some characteristics, such as adequate esthetics, high fracture strength and chemical stability. Currently, dental professionals have a large amount of ceramic systems to choose from, all of them having small differences regarding their chemistry, processing temperatures, mechanical strength and clinical applications. These differences lead to classification systems that are difficult to understand by professionals outside the dental field. The aim of this work is to review the current dental ceramic systems and present them from a compositional perspective to assure the comprehension of these materials by professionals who belong to the Biomedical Engineering field. A great effort was made to avoid classification methods imposed by manufacturers and to obtain a quick compilation of available information from the literature allowing the reader to have a general view of the stateoftheart on dental ceramics.},
	keywords = {Ceramic systems, Feldspathic porcelain, Glassceramics, Leucite, Mechanical and physical properties, Zirconium oxide},
	isbn = {00127353 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80051564798\&partnerID=40\&md5=2e79174a5238798dfcce9d2b5d7f29e0},
	author = {Santander, S.A. and Vargas, A.P. and Escobar, J.S. and Monteiro, F.J. and Tamayo, L.F.R.}
}
@article {2265,
	title = {Characterization of two DLC coatings for joint prosthesis: The role of albumin on the tribological behavior},
	journal = {Surface and Coatings TechnologySurf. Coat. Technol.},
	volume = {204},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.surfcoat.2010.04.022Language of Original Document: EnglishCorrespondence Address: Serro, A.P.; Centro de Qu{\'\i}mica Estrutural, Complexo I, Instituto Superior T{\'e}cnico, Av. Rovisco Pais, TU Lisbon, 1049-001 Lisboa, Portugal; email: anapaula.serro@ist.utl.ptReferences: Donnet, C., Erdemir, A., (2009), Springer Science \& Business Media, New YorkGutensohn, K., Beythien, C., Bau, J., Fenner, T., Grewe, P., Koester, R., Padmanaban, K., Kuehnl, P., (2000) Thromb. Res., 99, p. 577;Cadosch, D., Chan, E., Gautschi, O.P., Simmen, H.-P., Filgueira, L., (2009) J. Orthop. Res., 27, p. 841; 
Gillespie, W.L., Frampton, C.M.A., Henderson, R.J., Ryan, P.M., (1988) J. Bone Joint Surg. Br., 70 (4), p. 539; 
Roy, R.K., Lee, K.-R., (2007) J. Biomed. Mater. Res. B Appl. Biomater., 83 B (1), p. 72; 
Alakoski, E., Tiainen, V.-M., Soininen, A., Konttinen, Y.T., (2008) Open Orthop. J., 2, p. 43; 
Fisher, J., Firkins, P., Reeves, E.A., Hailey, J.L., Isaac, G.H., (1995) Proc. Inst. Mech. Eng., 209, p. 263; 
Sheeja, D., Tay, B.K., Lau, S.P., Nung, L.N., (2001) Surf. Coat. Technol., p. 410; 
Tiainen, V.-M., (2001) Diamond Relat. Mater., 10 (2), p. 153; 
O{\~n}ate, J.I., Comin, M., Braceras, I., Garcia, A., Viviente, J.L., Brizuela, M., Garagorri, N., Alava, J.I., (2001) Surf. Coat. Technol., p. 1056; 
Shi, B., Ajayi, O.O., Fenske, G., Erdemir, A., Liang, H., (2003) Wear, 255, p. 1015; 
Lappalainen, R., Selenius, M., Anttila, A., Konttinen, Y.T., Santavirta, S.S., (2003) J. Appl. Biomater., 66 B, p. 410; 
Sheeja, D., Tay, B.K., Nung, L.N., (2004) Diamond Relat. Mater., 13, p. 184; 
Xu, T., Pruitt, L., (1999) J. Mater. Sci. Mater. Med., 10, p. 83; 
Lappalainen, R., Anttila, A., Heinonen, H., (1998) Clin. Orthop., 352, p. 118; 
Saikko, V., Ahlroos, T., Calonius, O., Ker{\"a}nen, J., (2001) Biomaterials, 22, p. 1507; 
Sheeja, D., Tay, B.K., Lau, S.P., Nung, L.N., (2001) Surf. Coat. Technol., 146, p. 410; 
Affatato, S., Frigo, M., Toni, A., (2000) J. Biomed. Mater. Res., 53 (3), p. 221; 
Scholes, S.C., Unsworth, A., Goldsmit, A.A.J., (2000) Phys. Med. Biol., 45, p. 3721; 
Ahlroos, T., Saikko, V., (1997) Wear, 211, p. 113; 
Fedel, M., Motta, A., Maniglio, D., (2009) J. Biomed. Mater. Res., 90 B (1), p. 338; 
Lousiniana, S., Logothetidis, S., Laskarakisa, A., Gioti, M., (2007) Biomolec. Eng., 24 (1), p. 107; 
Li, B.G., Kang, Y.Q., Yin, G.F., Zheng, C.Q., (2007) Key Eng. Mater. High-Performance Ceram. IV, p. 1814; 
Fenske, G.R., (2009) Presented to Chicago Section STLE meeting, , M; 
Eryilmaz, O.L., Erdemir, A., (2008) Surf. Coat. Technol., 203, p. 750; 
Gerwin, N., Hops, C., Lucke, A., (2006) Adv. Drug Deliv. Rev., 58 (2), p. 226; 
Visco, A.M., Torrisi, L., Campo, N., Emanuele, U., Trifiro, A., Trimarchi, M., (2009) J. Biomed. Mater. Res. B Appl. Biomater., 89 B, p. 55; 
Ferrari, A.C., Robertson, J., (2001) Phys. Rev. B, 64, p. 75414; 
Ferrari, A.C., Robertson, J., (2000) Phys. Rev. B, 61, p. 14095; 
Zhou, B., Prorok, B.C., Erdemir, A., Eryilmaz, O., (2007) Diamond Relat. Mater., 16, p. 342; 
Zhou, B., Prorok, B.C., Erdemir, A., Eryilmaz, O., (2006) Diamond Relat. Mater., 15, p. 2051; 
Beeman, D., Silverman, J., Lynds, R., Anderson, M.R., (1984) Phys. Rev. B., 30 (2), p. 870; 
Cola{\c c}o, R., Serro, A.P., Eryilmaz, O.L., Erdemir, E., (2009) J. Phys. D Appl. Phys., 42, p. 85307; 
Paul, R., Das, S.N., Dalui, S., Gayen, R.N., Roy, R.K., Bhar, R., Pal, A.K., (2008) J. Phys. D Appl. Phys., 41 (5), p. 55309; 
Serro, A.P., Fernandes, A.C., Saramago, B., (1997) Colloids Surf. A Physicochem. Eng. Aspects, 125, p. 209; 
Serro, A.P., Degiampietro, K., Cola{\c c}o, R., Saramago, B., (2010) Saramago, Adsorption of albumin and sodium hyaluronate on UHMWPE: A QCM-D and AFM study, Colloids and Surfaces B: Biointerfaces, 78, p. 1; 
de Feijter, J.A., Benjamins, J., Veer, F.A., (1978) Biopolymers, 17, p. 1759; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., (2008) Surf. Int. Anal., 40 (12), p. 1529; 
Hook, F., Kasemo, B., Nylander, T., Fant, C., Sott, K., Elwing, H., (2001) Anal. Chem., 73 (24), p. 5796; 
Hook, F., Voros, J., Rodahl, M., Kurrat, R., Boni, P., Ramsden, J.J., Textor, M., Kasemo, B., (2002) Colloids Surf. B Biointerfaces, 24, p. 155; 
Cola{\c c}o, R., Gispert, M.P., Serro, A.P., Saramago, B., (2007) Tribol. Lett., 26 (2), p. 119; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., (2006) Wear, 260, p. 149; 
Serro, A.P., Completo, C., Cola{\c c}o, R., Dos Santos, F., Lobato da Silva, C., Cabral, J.M.S., Ara{\'u}jo, H., Saramago, B., (2009) Surf. Coat. Technol., 203, p. 3701; 
Widmer, M.R., Heuberger, M., V{\"o}r{\"o}s, J., Spencer, N.D., (2001) Tribol. Lett., 10, p. 111; 
Chen, X.M., Jin, Z.M., Fisher, J., (2008) Proc. Inst. Mech. Eng. Part J. Eng. Tribol., 222 (3), p. 513; 
Kumar, P., Oka, M., Ikeuchi, K., Shimizu, K., Yamamuro, T., Okumura, H., Kotoura, Y., (1991) J. Biomater. Mater. Res., 25, p. 813; 
Roba, M., Naka, M., Gautier, E., Spencer, N.D., Crockett, R., (2009) Biomaterials, 30, p. 2072; 
Karuppiah, K.S.K., Sundararajan, S., Xu, Z.-H., Li, X., (2006) Tribol. Lett., 22 (2), p. 181; 
Yang, C.-B., Fang, H.-W., Liu, H.-L., Chang, C.-H., Hsieh, M.-C., Lee, W.-M., Huang, H.-T., (2006) Chem. Phys. Lett., 431, p. 380; 
Fang, H.-W., Wang, H.-C., Tsai, T.-H., Tsai, W.-B., Hou, S.-Y., Liu, H.-L., Lee, W.-H., Huang, C.-H., (2008) J. Appl. Polym. Sci., 108, p. 2428; 
Zappone, B., Rosenberg, K.J., Israelachvili, J., (2007) Tribol. Lett., 26 (3), p. 191; 
Grill, A., (1997) Surf. Coat. Technol., (1-3), p. 507; 
Fontaine, J., Belin, M., Le Mogne, T., Grill, A., (2004) Tribol. Int., 37, p. 869; 
Sanchez-Lopez, J.C., Erdemir, A., Donnet, C., Rojas, T.C., (2003) Surf. Coat. Technol., 163, p. 444; 
Robertson, J., (2002) Mater. Sci. Eng., 37, p. 129
},
	month = {2010///},
	pages = {3451 - 3458},
	abstract = {A commonly used strategy for reduction of wear and friction of the sliding surfaces in prosthetic pairs is the use of hard coatings. DLCs (diamond like carbons) offer a promising solution, because these coatings, already used in other biomedical applications, exhibit a unique combination of chemical inertness, mechanical and tribological properties. In the present work we investigated the role of albumin, the most abundant protein in the periprosthetic fluid, on the tribological behavior of two DLCs with different compositions, against ultra-high molecular weight polyethylene (UHMWPE). The results are compared with those obtained with uncoated stainless steel surfaces. We have characterized the coatings by atomic force microscopy, X-ray diffraction, Raman spectroscopy, ultramicrohardness and wettability measurements. The adsorption of albumin onto the surfaces was investigated by ellipsometry and using a quartz crystal microbalance. Both DLCs adsorb a larger amount of protein than stainless steel. The tribological tests were performed in microtribometer aiming for a deeper understanding of the tribological mechanisms which occur at the submicrometric contact scale. Results showed no significant differences between the tribological behavior of the two coatings. Under the tested conditions, the tribological performance does not improve significantly with the application of the DLCs. Moreover, the presence of albumin on the lubricant leads to higher friction of the polymer against all counterfaces. This effect was attributed to the formation of bridges between the two sliding surfaces by the denaturated protein. The significant differences in the adsorbed amounts of protein onto the stainless steel and the two types of DLCs did not affect considerably the tribological behavior of these materials. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {Adsorbed amount, Adsorption, Albumin adsorption, Atomic force microscopy, Atomic spectroscopy, Biomedical applications, Chemical inertness, Corrosion resistant alloys, Diamond like carbon, DLC coatings, Friction, Hard coatings, Joint prostheses, Mechanical and tribological properties, Mechanical properties, Microtribology, Microtribometer, Nonmetallic matrix composites, Oxide minerals, Polyethylenes, Proteins, Quartz, Raman spectroscopy, Scanning electron microscopy, Sliding surface, Stainless steel, Stainless steel surface, Steel corrosion, Surfaces, Tribological behaviors, Tribological mechanism, Tribological performance, Tribological tests, Tribology, UHMW-PE, UHMWPE, Ultramicrohardness, Wear and friction, Wettability measurements, X ray diffraction},
	isbn = {02578972 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953715334\&partnerID=40\&md5=d724bda7404d809fed9bafb6a5a337e3},
	author = {Carapeto, A.P. and Serro, A.P. and Nunes, B.M.F. and Martins, M.C.L. and Todorovic, S. and Duarte, M.T. and Andr{\'e}, V. and Cola{\c c}o, R. and Saramago, B.}
}
@article {2244,
	title = {Chitosan-based gene delivery vectors targeted to the peripheral nervous system},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32874PubMed ID: 20734332Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; chitosan, 9012-76-4; imidazole, 1467-16-9, 288-32-4; macrogol, 25322-68-3; Cations; Chitosan, 9012-76-4; DNA, 9007-49-2; Polyethylene GlycolsTradenames: Chimarin, medicarb, SwedenManufacturers: medicarb, SwedenReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov Today, 1, pp. 177-185;Apfel, S.C., Neurotrophic factors in peripheral neuropathies: Therapeutic implications (1999) Brain Pathol, 9, pp. 393-413; 
Apfel, S.C., Is the therapeutic application of neurotrophic factors dead? (2002) Ann Neurol, 51, pp. 8-11; 
Glorioso, J.C., Mata, M., Fink, D.J., Therapeutic gene transfer to the nervous system using viral vectors (2003) J Neurovirol, 9, pp. 165-172; 
Fishman, P.S., Carrigan, D.R., Retrograde transneuronal transfer of the C-fragment of tetanus toxin (1987) Brain Res, 406, pp. 275-279; 
Evinger, C., Erichsen, J.T., Transsynaptic retrograde transport of fragment C of tetanus toxin demonstrated by immunohistochemical localization (1986) Brain Res, 380, pp. 383-388; 
Figueiredo, D.M., Hallewell, R.A., Chen, L.L., Fairweather, N.F., Dougan, G., Savitt, J.M., Parks, D.A., Fishman, P.S., Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport (1997) Exp Neurol, 145 (2 PART 1), pp. 546-554; 
Fishman, P.S., Savitt, J.M., Farrand, D.A., Enhanced CNS uptake of systemically administered proteins through conjugation with tetanus C-fragment (1990) J Neurol Sci, 98, pp. 311-325; 
Coen, L., Osta, R., Maury, M., Brulet, P., Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system (1997) Proc Natl Acad Sci USA, 94, pp. 9400-9405; 
Knight, A., Carvajal, J., Schneider, H., Coutelle, C., Chamberlain, S., Fairweather, N., Non-viral neuronal gene delivery mediated by the HC fragment of tetanus toxin (1999) Eur J Biochem, 259, pp. 762-769; 
Oliveira, H., Fernandez, R., Pires, L.R., Sim{\~o}es, S., Martins, M.C.L., Barbosa, M.A., P{\^e}go, A.P., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethilenimine) and tetanus toxin fragment c (2010) J Control Release, 143, pp. 350-358; 
Neu, M., Fischer, D., Kissel, T., Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives (2005) J Gene Med, 7, pp. 992-1009; 
Tang, G.P., Guo, H.Y., Alexis, F., Wang, X., Zeng, S., Lim, T.M., Ding, J., Wang, S., Low molecular weight polyethylenimines linked by beta-cyclodextrin for gene transfer into the nervous system (2006) J Gene Med, 8, pp. 736-744; 
Chollet, P., Favrot, M.C., Hurbin, A., Coll, J.L., Side-effects of a systemic injection of linear polyethylenimine-DNA complexes (2002) J Gene Med, 4, pp. 84-91; 
Shi, C., Zhu, Y., Ran, X., Wang, M., Su, Y., Cheng, T., Therapeutic potential of chitosan and its derivatives in regenerative medicine (2006) J Surg Res, 133, pp. 185-192; 
Mansouri, S., Lavigne, P., Corsi, K., Benderdour, M., Beaumont, E., Fernandes, J.C., Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: Strategies to improve transfection efficacy (2004) Eur J Pharm Biopharm, 57, pp. 1-8; 
Moreira, C., Oliveira, H., Pires, L.R., Simoes, S., Barbosa, M.A., Pego, A.P., Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone (2009) Acta Biomater, 5, pp. 2995-3006; 
Verma, A., Stellacci, F., Effect of surface properties on nanoparticle-cell interactions (2010) Small, 6, pp. 12-21; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
(1987) Guideline on Validation of the Limulus Amebocyte Lysate Test As An End-product Endotoxin Test for Human and Animal Parental Drugs, Biological Products, and Medical Devices, , US Department of Health and Human Services. US Department of Health and Human Services; 
MacUlotti, K., Genta, I., Perugini, P., Imam, M., Bernkop-Schnurch, A., Pavanetto, F., Preparation and in vitro evaluation of thiolated chitosan microparticles (2005) J Microencapsul, 22, pp. 459-470; 
Mao, H., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., August, J.T., Leong, K.W., Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency (2001) J Control Release, 70, pp. 399-421; 
Innes, N.P.T., Ogden, G.R., A technique for the study of endocytosis in human oral epithelial cells (1999) Arch Oral Biol, 44, pp. 519-523; 
O{\textquoteright}Brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) Eur J Biochem, 267, pp. 5421-5426; 
Ellman, G.L., Tissue sulfhydryl groups (1959) Arch Biochem Biophys, 82, pp. 70-77; 
Lee, D., Zhang, W., Shirley, S.A., Kong, X., Hellermann, G.R., Lockey, R.F., Mohapatra, S.S., Thiolated chitosan/DNA nanocomplexes exhibit enhanced and sustained gene delivery (2007) Pharm Res, 24, pp. 157-167; 
Loretz, B., Thaler, M., Bernkop-Schnurch, A., Role of sulfhydryl groups in transfection? A case study with chitosan-NAC nanoparticles (2007) Bioconjug Chem, 18, pp. 1028-1035; 
Deinhardt, K., Berninghausen, O., Willison, H.J., Hopkins, C.R., Schiavo, G., Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin 1 (2006) J Cell Biol, 174, pp. 459-471; 
Williamson, L.C., Neale, E.A., Bafilomycin A1 inhibits the action of tetanus toxin in spinal-cord neurons in cell-culture (1994) J Neurochem, 63, pp. 2342-2345; 
Bohnert, S., Schiavo, G., Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation (2005) J Biol Chem, 280, pp. 42336-42344
},
	month = {2010///},
	pages = {801 - 810},
	abstract = {A non-toxic, targeted, simple and efficient system that can specifically transfect peripheral sensorial neurons can pave the way towards the development of new therapeutics for the treatment of peripheral neuropathies. In this study chitosan (CH), a biodegradable polymer, was used as the starting material in the design of a multicomponent vector targeted to the peripheral nervous system (PNS). Polycation-DNA complexes were optimized using imidazole-and thiol-grafted CH (CHimiSH), in order to increase transfection efficiency and allow the formation of ligand conjugated nanocomplexes, respectively. The 50 kDa non-toxic fragment from the tetanus toxin (HC), shown to interact specifically with peripheral neurons and undergo retrograde transport, was grafted to the binary complex via a bi-functional poly(ethylene glycol) (HC-PEG) reactive for the thiol moieties present in the complex surface. The targeting of the developed nanocomplexes was assessed by means of internalization and transfection studies in the ND7/23 (neuronal) vs. NIH 3T3 (fibroblast) cell lines. Targeted transfection was further confirmed in dorsal root ganglion dissociated primary cultures. A versatile, multi-component nanoparticle system that successfully targets and transfects neuronal cell lines, as well as dorsal root ganglia (DRG) primary neuron cultures was obtained for the 1.0 (w/w) HC-PEG/DNA formulation. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {animal cell, Animals, article, Binary complexes, Biodegradable polymers, Carbohydrate Conformation, Carbohydrate Sequence, Cations, Cell culture, Cell lines, cell population, Cells, Cultured, Chitin, chitosan, Complex surface, DNA, DNA complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Ethylene Glycol, Female, Ganglia, Spinal, Gene delivery, gene delivery system, Gene delivery vectors, Gene therapy, Gene transfer, Gene Transfer Techniques, genetic transfection, Genetic Vectors, Grafting (chemical), Humans, imidazole, macrogol, materials testing, Mice, Molecular Sequence Data, Molecular Structure, mouse, Multicomponents, Nanocomplexes, Nanoparticle systems, nanoparticles, nerve cell culture, Neuron culture, Neuronal cell, NIH 3T3 Cells, nonhuman, Nontoxic fragment of tetanus toxin, Peripheral nervous system, Peripheral neuropathy, Polycations, Polyethylene Glycols, Polyethylene oxides, Pregnancy, Primary culture, rat, Rats, Rats, Wistar, spinal ganglion, Starting materials, Targeted, Tetanus toxin, Toxic materials, Transfection, Transfection efficiency},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78249240032\&partnerID=40\&md5=af9c3ca5ca712d53abb5e80f45ed09c0},
	author = {Oliveira, H. and Pires, L.R. and Fernandez, R. and Martins, M.C.L. and Sim{\~o}es, S. and P{\^e}go, A.P.}
}
@article {2250,
	title = {Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth},
	journal = {Nano LettersNano Lett.},
	volume = {10},
	year = {2010},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: Scopusdoi: 10.1021/nl1016909Language of Original Document: EnglishCorrespondence Address: Schlosshauer, B.; NMI Natural and Medical Sciences Institute, University of T{\"u}bingen, Markwiesenstrasse 55, 72770 Reutlingen, Germany; email: schlosshauer@nmi.deChemicals/CAS: chitosan, 9012-76-4; Chitosan, 9012-76-4; RNA, Small Interfering; rhoA GTP-Binding Protein, 3.6.5.2References: Terzis, J.K., Skoulis, T.G., Soucacos, P.N., (1995) Int. Angiol., 14, pp. 264-77;Schmidt, C.E., Leach, J.B., (2003) Annu. Rev. Biomed. Eng., 5, pp. 293-347; 
Stokols, S., Tuszynski, M.H., (2004) Biomaterials, 25 (27), pp. 5839-46; 
Goraltchouk, A., Scanga, V., Morshead, C.M., Shoichet, M.S., (2006) J. Controlled Release, 110 (2), pp. 400-7; 
Wu, J., Chiu, D.T., (1999) Ann. Plast. Surg., 43, pp. 661-667; 
Chen, M.S., Huber, A.B., Van Der Haar, M.E., Frank, M., Schnell, L., Spillmann, A.A., Christ, F., Schwab, M.E., (2000) Nature, 403 (6768), pp. 434-9; 
Grandpre, T., Nakamura, F., Vartanian, T., Strittmatter, S.M., (2000) Nature, 403 (6768), pp. 439-44; 
Liu, B.P., Fournier, A., Grandpre, T., Strittmatter, S.M., (2002) Science, 297 (5584), pp. 1190-3; 
McKerracher, L., David, S., Jackson, D.L., Kottis, V., Dunn, R.J., Braun, P.E., (1994) Neuron, 13 (4), pp. 805-11; 
Mukhopadhyay, G., Doherty, P., Walsh, F.S., Crocker, P.R., Filbin, M.T., (1994) Neuron, 13 (3), pp. 757-67; 
Wang, K.C., Koprivica, V., Kim, J.A., Sivasankaran, R., Guo, Y., Neve, R.L., He, Z., (2002) Nature, 417 (6892), pp. 941-4; 
Mi, S., Lee, X., Shao, Z., Thill, G., Ji, B., Relton, J., Levesque, M., Pepinsky, R.B., (2004) Nat. Neurosci., 7 (3), pp. 221-8; 
Park, J.B., Yiu, G., Kaneko, S., Wang, J., Chang, J., He, X.L., Garcia, K.C., He, Z., (2005) Neuron, 45 (3), pp. 345-51; 
Shao, Z., Browning, J.L., Lee, X., Scott, M.L., Shulga-Morskaya, S., Allaire, N., Thill, G., Mi, S., (2005) Neuron, 45 (3), pp. 353-9; 
Wang, K.C., Kim, J.A., Sivasankaran, R., Segal, R., He, Z., (2002) Nature, 420 (6911), pp. 74-8; 
Wong, S.T., Henley, J.R., Kanning, K.C., Huang, K.H., Bothwell, M., Poo, M.M., (2002) Nat. Neurosci., 5 (12), pp. 1302-8; 
Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., Tuschl, T., (2001) Nature, 411 (6836), pp. 494-8; 
Higuchi, H., Yamashita, T., Yoshikawa, H., Tohyama, M., (2003) Biochem. Biophys. Res. Commun., 301 (3), pp. 804-9; 
Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., Mello, C.C., (1998) Nature, 391 (6669), pp. 806-11; 
Tijsterman, M., Plasterk, R.H., (2004) Cell, 117 (1), pp. 1-3; 
De Fougerolles, A., Vornlocher, H.P., Maraganore, J., Lieberman, J., (2007) Nat. Rev. Drug Discovery, 6 (6), pp. 443-53; 
Shrivastava, N., Srivastava, A., (2008) Biotechnol. J., 3 (3), pp. 339-53; 
Soutschek, J., Akinc, A., Bramlage, B., Charisse, K., Constien, R., Donoghue, M., Elbashir, S., Vornlocher, H.P., (2004) Nature, 432 (7014), pp. 173-8; 
Takeshita, F., Ochiya, T., (2006) Cancer Sci., 97 (8), pp. 689-96; 
Bitko, V., Musiyenko, A., Shulyayeva, O., Barik, S., (2005) Nat. Med., 11 (1), pp. 50-5; 
Fjose, A., Drivenes, O., (2006) Birth Defects Res., C, 78 (2), pp. 150-71; 
Whitehead, K.A., Langer, R., Anderson, D.G., (2009) Nat. Rev. Drug Discovery, 8 (2), pp. 129-38; 
Liu, X., Howard, K.A., Dong, M., Andersen, M.O., Rahbek, U.L., Johnsen, M.G., Hansen, O.C., Kjems, J., (2007) Biomaterials, 28 (6), pp. 1280-8; 
Howard, K.A., Rahbek, U.L., Liu, X., Damgaard, C.K., Glud, S.Z., Andersen, M.O., Hovgaard, M.B., Kjems, J., (2006) Mol. Ther., 14 (4), pp. 476-84; 
Sell, S.A., McClure, M.J., Barnes, C.P., Knapp, D.C., Walpoth, B.H., Simpson, D.G., Bowlin, G.L., (2006) Biomed. Mater., 1 (2), pp. 72-80; 
Janes, K.A., Calvo, P., Alonso, M.J., (2001) Adv. Drug Delivery Rev., 47 (1), pp. 83-97; 
Pouton, C.W., Seymour, L.W., (2001) Adv. Drug Delivery Rev., 46 (1-3), pp. 187-203; 
Nel, A.E., Madler, L., Velegol, D., Xia, T., Hoek, E.M., Somasundaran, P., Klaessig, F., Thompson, M., (2009) Nat. Mater., 8 (7), pp. 543-57; 
Ishii, T., Okahata, Y., Sato, T., (2001) Biochim. Biophys. Acta, 1514 (1), pp. 51-64; 
Huang, M., Fong, C.W., Khor, E., Lim, L.Y., (2005) J. Controlled Release, 106 (3), pp. 391-406; 
Richardson, S.C., Kolbe, H.V., Duncan, R., (1999) Int. J. Pharm., 178 (2), pp. 231-43; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., (2003) Biomaterials, 24 (7), pp. 1255-64; 
Moghimi, S.M., Symonds, P., Murray, J.C., Hunter, A.C., Debska, G., Szewczyk, A., (2005) Mol. Ther., 11 (6), pp. 990-5; 
Koping-Hoggard, M., Tubulekas, I., Guan, H., Edwards, K., Nilsson, M., Varum, K.M., Artursson, P., (2001) Gene Ther., 8 (14), pp. 1108-21; 
Moreira, C., Oliveira, H., Pires, L.R., Simoes, S., Barbosa, M.A., Pego, A.P., (2009) Acta Biomater., 5 (8), pp. 2995-3006; 
Lietz, M., Dreesmann, L., Hoss, M., Oberhoffner, S., Schlosshauer, B., (2006) Biomaterials, 27 (8), pp. 1425-36; 
Ribeiro-Resende, V.T., Koenig, B., Nichterwitz, S., Oberhoffner, S., Schlosshauer, B., (2009) Biomaterials, 30 (29), pp. 5251-9; 
Andersen, M.O., Howard, K.A., Paludan, S.R., Besenbacher, F., Kjems, J., (2008) Biomaterials, 29 (4), pp. 506-12; 
Anchordoquy, T.J., Allison, S.D., Molina, M.C., Girouard, L.G., Carson, T.K., (2001) Drug Discovery Today, 6 (9), pp. 463-470; 
Atala, A., Bauer, S.B., Soker, S., Yoo, J.J., Retik, A.B., (2006) Lancet, 367 (9518), pp. 1241-6; 
Lin, C.Y., Schek, R.M., Mistry, A.S., Shi, X., Mikos, A.G., Krebsbach, P.H., Hollister, S.J., (2005) Tissue Eng., 11 (9-10), pp. 1589-98; 
Suggate, E.L., Ahmed, Z., Read, M.L., Eaton-Charnock, K., Douglas, M.R., Gonzalez, A.M., Berry, M., Logan, A., (2009) Mol. Cell Neurosci., 40 (4), pp. 451-62; 
Ahmed, Z., Dent, R.G., Suggate, E.L., Barrett, L.B., Seabright, R.J., Berry, M., Logan, A., (2005) Mol. Cell Neurosci., 28 (3), pp. 509-23; 
Fournier, A.E., Takizawa, B.T., Strittmatter, S.M., (2003) J. Neurosci., 23 (4), pp. 1416-23
},
	month = {2010///},
	pages = {3933 - 3939},
	abstract = {Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75\% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal, Animals, article, Cell Line, cell viability, chemistry, Chitin, chitosan, cytology, Drug delivery, genetic transfection, genetics, Local delivery, metabolism, Microstructured, nanobiofunctionalized implants, nanoparticle, nanoparticles, nerve cell, nerve regeneration, Nerve repair, neurite, Neurite outgrowth, Neurites, Neurons, Polymer filaments, Prostheses and Implants, prostheses and orthoses, rhoA GTP-Binding Protein, RhoA guanine nucleotide binding protein, RNA, RNA, Small Interfering, siRNA, small interfering RNA, Spinal cords, Transfection, ultrastructure},
	isbn = {15306984 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77958033530\&partnerID=40\&md5=6862e82e01c3aedf5423ebb14248c6ec},
	author = {Mittnacht, U. and Hartmann, H. and Hein, S. and Oliveira, H. and Dong, M. and P{\^e}go, A.P. and Kjems, J. and Howard, K.A. and Schlosshauer, B.}
}
@article {2283,
	title = {Comparison of a computer system evaluation of intrapartum cardiotocographic events and a consensus of clinicians.},
	journal = {Journal of perinatal medicineJ Perinat Med},
	volume = {38},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1515/JPM.2010.030PubMed ID: 20121542Language of Original Document: EnglishCorrespondence Address: Costa, M.A.email: cosantonia@gmail.com},
	month = {2010///},
	pages = {191 - 195},
	abstract = {AIMS: To compare between computer analysis of intrapartum cardiotocography (CTG) features by the Omniview-SisPorto 3.5 and a consensus of clinicians. METHODS: Agreement study using 50 consecutively acquired tracings (206 h of signals) with >60 min duration, <10\% signal loss and recorded in labor at term by internal fetal heart rate (FHR) monitoring. Tracings were divided into 10-min segments and independently analyzed by three experienced clinicians, in order to estimate the FHR baseline and identify periodic events. A consensus was reached using a three round Delphi procedure. Results were compared with the analysis provided by the Omniview-SisPorto 3.5 system. RESULTS: For baseline estimation, agreement between the computer and the consensus was high [intraclass correlation coefficient (ICC)=0.85; 95\% confidence interval (CI) 0.46-0.93], with a mean difference of 3.7 bpm (limits of agreement -4.4-11.9 bpm), and 99\% of differences under 15 bpm. A concordant identification was observed in 71\% of accelerations (95\% CI: 69\%-73\%), 68\% of decelerations (95\% CI: 66\%-70\%), and 87\% of uterine contractions (95\% CI: 85\%-89\%). CONCLUSIONS: A high agreement was observed between the Omniview-SisPorto 3.5 and a consensus of clinicians in evaluation of intrapartum CTG baseline, accelerations, decelerations and uterine contractions.},
	keywords = {algorithm, Algorithms, article, Cardiotocography, comparative study, computer assisted diagnosis, Diagnosis, Computer-Assisted, Female, Fetal Heart, Fetal monitoring, fetus heart, fetus monitoring, human, Humans, instrumentation, methodology, physician, Physicians, physiology, Pregnancy},
	isbn = {16193997 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953038696\&partnerID=40\&md5=bca187de4ffea0b2f2c4d2dcfb2140d9},
	author = {Costa, M.A. and Ayres-de-Campos, D. and Machado, A.P. and Santos, C.C. and Bernardes, J.}
}
@article {2243,
	title = {Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping},
	journal = {Microscopy and MicroanalysisMicrosc. Microanal.},
	volume = {16},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: MIMIFdoi: 10.1017/S1431927610094158PubMed ID: 20964878Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Faculdade de Engenharia, Departamento de Engenharia Metalrgica e Materiais, Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; Silicon Dioxide, 7631-86-9References: Albrektsson, T., Sennerby, L., Wennerberg, A., State of the art of oral implants (2000) Periodontology, 47, pp. 15-26;Albrektsson, T., Wennerberg, A., Oral implant surfaces: Part 1-Review focusing on topographic and chemical properties of different surfaces and in vivo responses to them (2004) Int J Prosthodont, 17 (5), pp. 536-543; 
Batista, L.H., Junior, J.G., Silva, M.F., Tonholo, J., Atomic force microscopy of removal of dentin smear layers (2007) Microsc Microanal, 13 (4), pp. 245-250; 
Baudino, T.A., McFadden, A., Fix, C., Hastings, J., Price, R., Borg, T.K., Cell patterning: Interaction of cardiac myocytes and fibroblasts in three-dimensional culture (2008) MicroscMicroanal, 14 (2), pp. 117-125; 
Bohil, A.B., Robertson, B.W., Cheney, R.E., Myosin-X is a molecular motor that functions in filopodia formation (2006) Proc Natl Acad Sci USA, 103 (33), pp. 12411-12416; 
Brunette, D.M., Kenner, G.S., Gould, T.R., Grooved titanium surfaces orient growth and migration of cells from human gingival explants (1983) J Dent Res, 62 (10), pp. 1045-1048; 
Curtis, A., Wilkinson, C., Topographical control of cells (1997) Biomaterials, 18 (24), pp. 1573-1583; 
Curtis, A.S., Cell reactions with biomaterials: The microscopies (2001) Eur Cell Mater, 1, pp. 59-65; 
Dalby, M.J., Riehle, M.O., Johnstone, H., Affrossman, S., Curtis, A.S., Investigating the limits of filopodial sensing: A brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia (2004) Cell Biol Int, 28 (3), pp. 229-236; 
Dur{\'a}n, A., Conde, A., Coedo, A.G., Dorado, T., Garc{\'\i}a, C., Cer{\'e}, S., Sol-gel coatings for protection and bioactiva- tion of metals used in orthopaedic devices (2004) J Mater Chem, 14 (14), pp. 2282-2290; 
Falconnet, D., Csucs, G., Grandin, H.M., Textor, M., Surface engineering approaches to micropattern surfaces for cell-based assays (2006) Biomaterials, 27 (16), pp. 3044-3063; 
Gallardo, J., Galliano, P., Dur{\'a}n, A., Thermal evolution of hybrid sol-gel silica coatings: A structural analysis (2000) J Sol-Gel Sci Technol, 19 (1), pp. 393-397; 
Gallardo, J., Galliano, P., Moreno, R., Dur{\'a}n, A., Bioactive sol-gel coatings for orthopaedic prosthesis (2000) J Sol-Gel Sci Technol, 19 (1-3), pp. 107-111; 
Gallego, D., Ferrell, N., Sun, Y., Hansford, D., Multilayer micromolding of degradable polymer tissue engineering scaffolds (2008) Mater Sci Eng C, 28 (3), pp. 353-358; 
Gallego-Perez, D., Higuita-Castro, N., Sharma, S., Reen, R.K., Palmer, A.F., Gooch, K.J., Lee, L.J., Hansford, D.J., High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform (2010) Lab Chip, 10 (6), pp. 775-782; 
Garcia, C., Cer{\'e}, S., Dur{\'a}n, A., Bioactive coatings prepared by sol-gel on stainless steel 316L (2004) J Non-Cryst Solids, 348, pp. 218-224; 
Healy, K.E., Thomas, C.H., Rezania, A., Kim, J.E., McKeown, P.J., Lom, B., Hockberger, P.E., Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry (1996) Biomaterials, 17 (2), pp. 195-208; 
Ito, Y., Surface micropatterning to regulate cell functions (1999) Biomaterials, 20 (23-24), pp. 2333-2342; 
Kim, E.J., Boehm, C.A., Mata, A., Fleischman, A.J., Muschler, G.F., Roy, S., Post microtextures accelerate cell proliferation and osteogenesis (2010) Acta Biomater, 6 (1), pp. 160-169; 
Lee, E.-J., Lee, S.H., Kim, H.W., Kong, Y.M., Kim, H.-E., Fluoridated apatite coatings on titanium obtained by electronbeam deposition (2005) Biomaterials, 26 (18), pp. 3843-3851; 
Lim, J.Y., Topographic control of cell response to synthetic materials (2009) Tissue Eng Regen Med, 6 (1), pp. 365-370; 
Marzolin, C., Smith, S.P., Prentiss, M., Whitesides, G.M., Fabrication of glass microstructures by micro-molding of sol-gel precursors (1998) Adv Mater, 10 (8), pp. 571-574; 
Mata, A., Boehm, C., Fleischman, A.J., Muschler, G., Roy, S., Growth of connective tissue progenitor cells on microtextured polydimethylsiloxane surfaces (2002) J Biomed Mater Res, 62 (4), pp. 499-506; 
Mata, A., Kim, E.J., Boehm, C.A., Fleischman, A.J., Muschler, G.F., Roy, S., A three-dimensional scaffold with precise micro-architecture and surface micro-textures (2009) Biomaterials, 30 (27), pp. 4610-4617; 
Meirelles, L., Currie, F., Jacobsson, M., Albrektsson, T., Wennerberg, A., The effect of chemical and nanotopographical modifications on the early stages of osseointegration (2008) Int J Oral Maxillofac Implants, 23 (4), pp. 641-647; 
Nayab, S.N., Jones, F.H., Olsen, I., Effects of calcium ion implantation on human bone cell interaction with titanium (2005) Biomaterials, 26 (23), pp. 4717-4727; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Cells spreading on micro-fabricated silica thin film coatings (2009) Microsc Microanal, 15 (S3), pp. 77-78; 
Perrie, W., Rushton, A., Gill, M., Fox, P., O{\textquoteright}Neill, W., Femtosecond laser micro-structuring of alumina ceramic (2005) Appl Surf Sci, 248 (1-4), pp. 213-217; 
Rhee, S.W., Taylor, A.M., Tu, C.H., Cribbs, D.H., Cotman, C.W., Jeon, N.L., Patterned cell culture inside microfluidic devices (2005) Lab Chip, 5 (1), pp. 102-107; 
Rompen, E., Domken, O., Degidi, M., Pontes, A.E., Piattelli, A., The effect of material characteristics, of surface topography and of implant components and connections on soft tissue integration: A literature review (2006) Clin Oral Implants Res, 17 (S2), pp. 55-67; 
Sennerby, L., Dental implants: Matters of course and controversies (2000) Periodontol, 47, pp. 9-14; 
Silva, P.L., Santos, J.D., Monteiro, F.J., Knowles, J.C., Adhesion and microstructural characterization of plasmasprayed hydroxyapatite/glass ceramic coatings onto Ti-6A1-4V substrates (1998) Surf Coat Technol, 102 (3), pp. 191-196; 
Tan, J., Saltzman, W.M., Biomaterials with hierarchically defined micro- and nanoscale structure (2004) Biomaterials, 25 (17), pp. 3593-3601; 
Teixeira, S., Monteiro, F.J., Ferraz, M.P., Vilar, R., Eugenio, S., Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies (2007) J Biomed Mater Res A, 81 (4), pp. 920-929; 
Vorobyev, A.Y., Guo, C., Femtosecond laser structuring of titanium implants (2007) Appl Surf Sci, 253 (17), pp. 7272-7280; 
Wang, Y.C., Ho, C.C., Micropatterning of proteins and mammalian cells on biomaterials (2004) Faseb J, 18 (3), pp. 525-527; 
Wennerberg, A., Albrektsson, T., Andersson, B., Krol, J.J., A histomorphometric study of screw shaped and removal torque titanium implants with three different surface topographies (1995) Clin Oral Impl Res, 6 (1), p. 24; 
Xia, Y., Whitesides, G.M., Soft lithography (1998) Ann Rev Mater Sci, 28 (1), pp. 153-184; 
Xia, Y., Whitesides, G.M., Soft lithography (1998) Angew Chem Int Edit, 37 (5), pp. 550-575; 
Zinger, O., Zhao, G., Schwartz, Z., Simpson, J., Wieland, M., Landolt, D., Boyan, B., Differential regulation of osteoblasts by substrate microstructural features (2005) Biomaterials, 26 (14), pp. 1837-1847
},
	month = {2010///},
	pages = {670 - 676},
	abstract = {While there has been rapid development of microfabrication techniques to produce high-resolution surface modifications on a variety of materials in the last decade, there is still a strong need to produce novel alternatives to induce guided tissue regeneration on dental implants. High-resolution microscopy provides qualitative and quantitative techniques to study cellular guidance in the first stages of cell-material interactions. The purposes of this work were (1) to produce and characterize the surface topography of isotropic and anisotropic microfabricated silica thin films obtained by sol-gel processing, and (2) to compare the in vitro biological behavior of human bone marrow stem cells on these surfaces at early stages of adhesion and propagation. The results confirmed that a microstamping technique can be used to produce isotropic and anisotropic micropatterned silica coatings. Atomic force microscopy analysis was an adequate methodology to study in the same specimen the sintering derived contraction of the microfabricated coatings, using images obtained before and after thermal cycle. Hard micropatterned coatings induced a modulation in the early and late adhesion stages of cell-material and cell-cell interactions in a geometry-dependent manner (i.e., isotropic versus anisotropic), as it was clearly determined, using scanning electron and fluorescence microscopies. {\textcopyright} Microscopy Society of America 2010.},
	keywords = {AFM, Atomic force microscopy, audiovisual equipment, cell adhesion and proliferation, Cell Culture Techniques, cell proliferation, conference paper, culture technique, cytology, Fluorescence microscopy, Guided tissue regeneration, human, Humans, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Microscopy, Fluorescence, microstamping, Motion Pictures as Topic, myeloid progenitor cell, Myeloid Progenitor Cells, Scanning electron microscopy, SEM, silica sol-gel films, silicon dioxide, Soft lithography, Surface Properties, surface property, Tissue regeneration, tissue scaffold, Tissue Scaffolds},
	isbn = {14319276 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79451471917\&partnerID=40\&md5=c68d4037bb3535a092d46c2602a8b4bd},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Ferrell, N. and Fernandes, M.H. and Hansford, D. and Monteiro, F.J.}
}
@article {2279,
	title = {The effect of immobilization of thrombin inhibitors onto self-assembled monolayers on the adsorption and activity of thrombin},
	journal = {BiomaterialsBiomaterials},
	volume = {31},
	year = {2010},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2010.01.097PubMed ID: 20153046Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: thrombin, 9002-04-4; Albumins; Gold, 7440-57-5; Immobilized Proteins; Iodine Radioisotopes; Peptides; Solutions; Thrombin, 3.4.21.5References: Gorbet, M.B., Sefton, M.V., Biomaterial-associated thrombosis: roles of coagulation factors, complement, platelets and leukocytes (2004) Biomaterials, 25 (26), pp. 5681-5703;Ratner, B.D., Hoffman, A.F., Schoen, F.J., Lemons, J.L., (2004) Biomaterials science - an introduction to materials in medicine. 2nd ed., , Elsevier Academic Press, San Diego, CA, USA; 
Bode, W., Structure and interaction modes of thrombin (2006) Blood Cells Mol Dis, 36 (2), pp. 122-130; 
Huntington, J.A., Molecular recognition mechanisms of thrombin (2005) J Thromb Haemost, 3 (8), pp. 1861-1872; 
Schwienhorst, A., Direct thrombin inhibitors - a survey of recent developments (2006) Cell Mol Life Sci, 63 (23), pp. 2773-2791; 
Tseng, P.-Y., Rele, S.S., Sun, X.-L., Chaikof, E.L., Membrane-mimetic films containing thrombomodulin and heparin inhibit tissue factor-induced thrombin generation in a flow model (2006) Biomaterials, 27 (12), pp. 2637-2650; 
Sperling, C., Salchert, K., Streller, U., Werner, C., Covalently immobilized thrombomodulin inhibits coagulation and complement activation of artificial surfaces in vitro (2004) Biomaterials, 25 (21), pp. 5101-5113; 
Lin, J.C., Tseng, S.M., Surface characterization and platelet adhesion studies on polyethylene surface with hirudin immobilization (2001) J Mater Sci Mater Med, 12 (9), pp. 827-832; 
Wendel, H.P., Ziemer, G., Coating-techniques to improve the hemocompatibility of artificial devices used for extracorporeal circulation (1999) Eur J Cardiothorac Surg, 16 (3), pp. 342-350; 
Werner, C., Maitz, M.F., Sperling, C., Current strategies towards hemocompatible coatings (2007) J Mater Chem, 17 (32), pp. 3376-3384; 
Weitz, J.I., Crowther, M., Direct thrombin inhibitors (2002) Thromb Res, 106 (3), pp. V275-V284; 
Di Nisio, M., Middeldorp, S., B{\"u}ller, H.R., Direct thrombin inhibitors (2005) N Engl J Med, 353 (10), pp. 1028-1040; 
Stone, S.R., Hofsteenge, J., Kinetics of the inhibition of thrombin by hirudin (1986) Biochemistry, 25 (16), pp. 4622-4628; 
Grutter, M.G., Priestle, J., Rahuel, J., Grossenbacher, H., Bode, W., Hofsteenge, J., Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition (1990) EMBO J, 9 (8), pp. 2361-2365; 
Lahann, J., Pl{\"u}ster, W., Klee, D., Gattner, H.G., H{\"o}cker, H., Immobilization of the thrombin inhibitor r-hirudin conserving its biological activity (2001) J Mater Sci Mater Med, 12 (9), pp. 807-810; 
Phaneuf, M.D., Berceli, S.A., Bide, M.J., Quist, W.C., LoGerfo, F.W., Covalent linkage of recombinant hirudin to poly(ethylene terephthalate) (Dacron): creation of a novel antithrombin surface (1997) Biomaterials, 18 (10), pp. 755-765; 
Wyers, M.C., Phaneuf, M.D., Rzucidlo, E.M., Contreras, M.A., Logerfo, F.W., Quist, W.C., In~vivo assessment of a novel Dacron surface with covalently bound recombinant hirudin (1999) Cardiovasc Pathol, 8 (3), pp. 153-159; 
Maraganore, J.M., Bourdon, P., Jablonski, J., Ramachandran, K.L., Fenton Ii, J.W., Design and characterization of hirulogs: a novel class of bivalent peptide inhibitors of thrombin (1990) Biochemistry, 29 (30), pp. 7095-7101; 
Maraganore, J.M., Chao, B., Joseph, M.L., Jablonski, J., Ramachandran, K.L., Anticoagulant activity of synthetic hirudin peptides (1989) J Biol Chem, 264 (15), pp. 8692-8698; 
Sun, X., Sheardown, H., Tengvall, P., Brash, J.L., Peptide modified gold-coated polyurethanes as thrombin scavenging surfaces (2000) J Biomed Mater Res, 49 (1), pp. 66-78; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111 (1), pp. 321-335; 
Herrwerth, S., Eck, W., Reinhardt, S., Grunze, M., Factors that determine the protein resistance of oligoether self-assembled monolayers - internal hydrophilicity, terminal hydrophilicity, and lateral packing density (2003) J Am Chem Soc, 125 (31), pp. 9359-9366; 
Lottenberg, R., Christensen, U., Jackson, C.M., Coleman, P.L., Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates (1981) Meth Enzymol, 80 (PART C), pp. 341-361; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) J~Biomed Mater Res A, 67 A (1), pp. 158-171; 
Martins, M.C.L., Curtin, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res A, 88 (1), pp. 162-173; 
(1993) Iodine-125. A guide to radioiodination techniques, , Amersham Life Science, Little Chalfort; 
Rahmelow, K., Hubner, W., Ackermann, T., Infrared absorbances of protein side chains (1998) Anal Biochem, 257 (1), pp. 1-11; 
Barth, A., The infrared absorption of amino acid side chains (2000) Prog Biophys Mol Biol, 74 (3-5), pp. 141-173; 
Li, L., Chen, S., Zheng, J., Ratner, B.D., Jiang, S., Protein adsorption on oligo(ethylene glycol)-terminated alkanethiolate self-assembled monolayers: the molecular basis for nonfouling behavior (2005) J Phys Chem B, 109 (7), pp. 2934-2941; 
Beamson, G., Briggs, D., (1992) High resolution XPS of organic polymers, , John Wiley and Sons, Ltd;, New York; 
Adden, N., Gamble, L.J., Castner, D.G., Hoffmann, A., Gross, G., Menzel, H., Phosphonic acid monolayers for binding of bioactive molecules to titanium surfaces (2006) Langmuir, 22 (19), pp. 8197-8204; 
Sousa, S.R., Moradas-Ferreira, P., Saramago, B., Viseu Melo, L., Barbosa, M.A., Human serum albumin adsorption on TiO 2 from single protein solutions and from plasma (2004) Langmuir, 20 (22), pp. 9745-9754; 
Di Cera, E., Thrombin interactions (2003) Chest, 124 (SUPPL. 3), pp. 11S-17S; 
Markwardt, F., Historical perspective of the development of thrombin inhibitors (2002) Pathophysiol Haemost Thromb, 32 (SUPPL. 3), pp. 15-22; 
Hearn, M.T., 1,1'-Carbonyldiimidazole-mediated immobilization of enzymes and affinity ligands (1987) Methods Enzymol, 135, pp. 102-117; 
St{\"o}llner, D., Scheller, F.W., Warsinke, A., Activation of cellulose membranes with 1,1'-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors (2002) Anal Biochem, 304 (2), pp. 157-165; 
Martins, M.C.L., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on Cibacron Blue F3G-A immobilized onto oligo(ethylene glycol)- terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14 (11), pp. 945-954; 
Dee, K.C., Puleo, D.A., Bizios, R., (2002) An introduction to tissue-biomaterial interactions, , Wiley-Liss, Inc; 
Malmsten, M., Lassen, B., Competitive adsorption at hydrophobic surfaces from binary protein systems (1994) J Colloid Interface Sci, 166 (2), pp. 490-498
},
	month = {2010///},
	pages = {3772 - 3780},
	abstract = {Thrombus formation is the major problem associated with biomaterials for blood contact medical devices. The immobilization of inhibitors to thrombin, a serine protease that plays a central role on the coagulation system, on the surface of biomaterials should be a good strategy to avoid blood clotting and increase their hemocompatibility. The aim of this work is the design of a nanostructured surface with capacity to adsorb and inactivate thrombin. The pentapeptide sequence d-Phenylalanine-Proline-Arginine-Proline-Glycine (fPRPG), that was described as a thrombin inhibitor, was immobilized onto tetra(ethylene glycol) terminated self-assembled monolayers (EG4-SAMs). Surface containing different amounts of fPRPG were prepared using different concentrations of N,N'-Carbonyldiimidazole (CDI) during immobilization. The efficiency of fPRPG immobilization was followed using ellipsometry, contact angle measurements, Infrared reflection absorption spectroscopy (IRRAS) and X-ray photoelectron spectroscopy (XPS). Thrombin adsorption was quantified using radiolabelled thrombin and its activity in solution and after adsorption on the developed surfaces was assessed using a chromogenic assay. It was found that, although the immobilization of fPRPG on to EG4-SAMs does not increase its selectivity to thrombin, the activity of the adsorbed thrombin was inhibited in a peptide concentration dependent way. We concluded that SAMs with fPRPG immobilized in high amounts can be used as thrombin-inhibitor surfaces, which is a good step on the development of new surfaces for blood contact devices. {\textcopyright} 2010 Elsevier Ltd. All rights reserved.},
	keywords = {Absorption spectroscopy, Adsorption, adsorption kinetics, Albumins, Amino Acid Sequence, Amino acids, Angle measurement, article, Biomaterials, Blood, Blood clotting, Blood contact, Carbonyldiimidazole, Coagulation, Coagulation system, concentration (parameters), Concentration-dependent, Contact angle, D-phenylalanine, ellipsometry, Ethylene, Ethylene Glycol, ethylene glycol derivative, gold, Hemocompatibility, Humans, imidazole derivative, Immobilized Proteins, Infrared reflection absorption spectroscopy, infrared spectroscopy, Iodine Radioisotopes, isotope labeling, Medical Devices, monolayer culture, nanoanalysis, Nanostructured surface, peptide, Peptide immobilization, Peptides, phenylalanylprolylarginylprolylglycine, Phosphatases, priority journal, Protein adsorption, protein assembly, protein function, protein immobilization, quantitative study, Sams, Self assembled monolayers, Self-assembled monolayers, Serine protease, Solubility, Solutions, Spectroscopy, Fourier Transform Infrared, Surface functionalization, Surface Properties, Tetra, tetra(ethylene glycol), Thrombin, thrombin inhibitor, Thrombin inhibitors, Thrombus formation, unclassified drug, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77649271932\&partnerID=40\&md5=f7180daf23d326319cf6d8dbf0eac77c},
	author = {Freitas, S.C. and Barbosa, M.A. and Martins, M.C.L.}
}
@article {2284,
	title = {Effect of nanoscale topography on fibronectin adsorption, focal adhesion size and matrix organisation},
	journal = {Colloids and Surfaces B: BiointerfacesColloids Surf. B Biointerfaces},
	volume = {77},
	year = {2010},
	note = {Cited By (since 1996): 25Export Date: 21 November 2012Source: ScopusCODEN: CSBBEdoi: 10.1016/j.colsurfb.2010.01.021PubMed ID: 20185279Language of Original Document: EnglishCorrespondence Address: Rico, P.; Center for Biomaterials and Tissue Engineering, Universidad Polit{\'e}cnica de Valencia, 46022 Valencia, Spain; email: parico@upvnet.upv.esChemicals/CAS: fibronectin, 86088-83-7; polylactic acid, 26100-51-6; polystyrene, 9003-53-6; Fibronectins; ProteinsReferences: Spie, J., (2002) Ann. N. Y. Acad. Sci., 961, p. 1;Griffin, L., Naughton, G., (2002) Science, 259, p. 1009; 
Grinnell, F., (1986) J. Cell Biol., 103, p. 2697; 
Hynes, R.O., (2002) Cell, 110, p. 673; 
Wong, J.Y., Leach, J.B., Brown, X.Q., (2004) Surf. Sci., 570, p. 119; 
Schwarz, U.S., Bischofs, I.B., (2005) Med. Eng. Phys., 27, p. 763; 
Ohara, P.T., Buck, R.C., (1979) Exp. Cell Res., 121, p. 235; 
Lim, J.Y., Donahue, H.J., (2007) Tissue Eng., 13, p. 1879; 
Affrossman, S., Jerome, R., O{\textquoteright}Neill, S.A., Schmitt, T., Stamm, M., (2000) Colloid Polym. Sci., 278, p. 888; 
Denis, F.A., Hanarp, P., Sutherland, D.S., Dufrene, Y.F., (2002) Nanoletters, 2, p. 1419; 
Vieu, C., Carcenac, F., Pepin, A., Chen, Y., Mejias, M., Lebib, A., Manin-Ferlazzo, L., Launois, H., (2000) Appl. Surf. Sci., 164, p. 111; 
Kriparamanan, R., Aswath, P., Zhou, A., Tang, L., Nguyen, K.T., (2006) J. Nanosci. Nanotechnol., 6, p. 1905; 
Dalby, M.J., Giannaras, D., Riehle, M.O., Gadegaard, N., Affrossman, S., Curtis, A.S.G., (2004) Biomaterials, 25, p. 77; 
Lim, J.Y., Dreiss, D., Zhou, Z., Hansen, J.C., Siedlecki, C.A., Hengstebeck, R.W., Cheng, J., Donahue, H.J., (2007) Biomaterials, 28, p. 1787; 
Dalby, M.J., Hart, A., Yarwood, S.J., (2008) Biomaterials, 29, p. 282; 
Zinger, O., Zhao, G., Schwartz, Z., Simpson, J., Wieland, M., Landlolt, D., Boyan, B., (2005) Biomaterials, 26, p. 1837; 
Dalby, M.J., Gadegaard, N., Tare, R., Andar, A., Riehle, M.O., Herzyk, P., Wilkinson, C.D.W., Oreffo, R.O.C., (2007) Nat. Mater., 9, p. 997; 
Dalby, M.J., McCloy, D., Robertson, M., Wilkinson, C.D.W., Oreffo, R.O.C., (2006) Biomaterials, 27, p. 1306; 
Pirouz-Dolatshahi, A., Rechendorff, K., Hovgaard, M.B., Foss, M., Chevallier, J., Besenbacher, F., (2008) Colloid Surf. B, 66, p. 53; 
Khor, H.L., Kukula, H., Tamada, K., Knoll, W., Moeller, M., Hutmacher, D.W., (2007) Biomacromolecules, 8, p. 1530; 
Cai, K., Bossert, J., Jandt, K., (2006) Colloid Surf. B, 49, p. 136; 
(1993), p. 64. , Iodine-125, Amersham International, Little Chalfont, Buckinghamshire, U.K.Sousa, S.R., Br{\'a}s, M., Moradas-Ferreira, P., Barbosa, M.A., (2007) Langmuir, 23, p. 7046; 
Otsu, N.A., (1979) IEEE Trans. Syst. Man Cybern., 9, p. 62; 
Gonzalez, R.C., Woods, R.E., Eddins, S.L., (2003) Digital Image Processing Using MATLAB, , Prentice-Hall, Upper Saddle River, NJ; 
Gonzalez, R.C., Woods, R.E., (2007) Digital Image Processing, , Prentice-Hall, Upper Saddle River, NJ; 
(2006), MATLAB Image Processing Toolbox User{\textquoteright}s Guide, The MathWorks, Inc., Natick, MAAllen, L.T., Tosetto, M., Miller, I.S., O{\textquoteright}Connor, D.P., Penney, S.C., Lynch, I., Keenan, A.K., Gallagher, W.M., (2006) Biomaterials, 27, p. 3096; 
Gugutkov, D., Altankov, G., Rodr{\'\i}guez Hern{\'a}ndez, J.C., Monle{\'o}n Pradas, M., Salmer{\'o}n S{\'a}nchez, M., (2010) J. Biomed. Mater. Res. A, 92, p. 322; 
Rico, P., Rodr{\'\i}guez Hern{\'a}ndez, J.C., Moratal, D., Monle{\'o}n Pradas, M., Salmer{\'o}n S{\'a}nchez, M., (2009) Macromol. Biosci., 9, p. 766; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J., (2003) J. Biomed. Mater. Res. A, 66, p. 247; 
Lee, M.H., Ducheyne, P., Lynch, L., Boettiger, D., Composto, R.J., (2006) Biomaterials, 27, p. 1907; 
Costa Mart{\'\i}nez, E., Rodr{\'\i}guez Hern{\'a}ndez, J.C., Machado, M., Mano, J., G{\'o}mez Ribelles, J.L., Monle{\'o}n Pradas, M., Salmer{\'o}n S{\'a}nchez, M., (2008) Tissue Eng., 14, p. 1751; 
Lim, J.Y., Hansen, J.C., Siedlecki, C.A., Hengstebeck, R.A., Cheng, J., Winograd, N., (2005) Biomacromolecules, 6, p. 3319; 
Liu, X., Lim, J.Y., Donahue, H.J., Dhurjati, R., Maestro, A.M., Vogler, E.A., (2007) Biomaterials, 28, p. 4535; 
Dalby, M.J., Yarwood, S.J., Riehle, M.O., Johnstone, H.J., Affressman, S., Curtis, A.S., (2002) Exp. Cell Res., 276, p. 1; 
Dalby, M.J., Childs, S., Riehle, M.O., Johnston, H.J.H., Affrossman, S., (2003) Biomaterials, 24, p. 927; 
Dalby, M.J., McCloy, D., Robertson, M., Agheli, H., Sutherland, D., Affrossman, S., Oreffo, R.O.C., (2006) Biomaterials, 27, p. 2980; 
Dalby, M.J., (2005) Med. Eng. Phys., 27, p. 730; 
Galbraith, C.G., Yamada, K.M., Sheetz, M.P., (2002) J. Cell Biol., 159, p. 695; 
Balaban, N.Q., Schwarz, U.S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., (2001) Nat. Cell Biol., 3, p. 466; 
Pegueroles, M., Aparicio, C., Bosio, M., Engel, E., Gil, F.J., Planell, J.A., Altankov, G., Acta Biomater. (). doi:10.1016/j.actbio.2009.07.030Altankov, G., Groth, T., (1994) J. Mater. Sci. Mater. Med., 5, p. 732; 
Altankov, G., Groth, T., (1996) J. Mater. Sci. Mater. Med., 7, p. 425; 
Altankov, G., Grinnell, F., Groth, T., (1996) J. Biomed. Mater. Res., 30, p. 385; 
Tzoneva, R., Groth, T., Altankov, G., Paul, D., (2002) J. Mater. Sci. Mater. Med., 13, p. 1235; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., (2005) Biomaterials, 26, p. 3891; 
Luthen, F., Lange, R., Becker, P., Rychly, J., Beck, U., Nebe, J.G.B., (2005) Biomaterials, 26, p. 2423; 
Geiger, B., Bershadsky, A., Pankov, R., Yamada, K.M., (2001) Nat. Rev. Mol. Cell Biol., 2, p. 793; 
Altankov, G., Groth, T., (1996) J. Biomater. Sci. Polym. Ed., 8, p. 299
},
	month = {2010///},
	pages = {181 - 190},
	abstract = {Phase separation of PLLA/PS (50/50, w/w) solutions during a spin-casting process gives rise to well-defined nanotopographies of 14, 29 and 45. nm deep pits depending on the concentration of the solution. Their influence on the biological activity of fibronectin (FN) was investigated. FN adsorption was quantified by radiolabelling the protein. The amount of adsorbed FN was higher on the 14. nm deep pit nanotopography than on the other two surfaces. FN distribution between valleys and peaks was investigated by AFM combined with image analysis. FN tends to adsorb preferentially on the valleys of the nanotopography only for the 14. nm system and when adsorbed from solutions of concentration lower than 10μg/ml. Higher concentration of the FN solution leads to evenly distribution of the protein throughout the surface; moreover, there is no difference in the distribution of the protein between valleys and peaks for the other two systems (29 and 45. nm) irrespective of the concentration of the FN solution. The biological activity of the adsorbed protein layer was assessed by investigating MC3T3 osteoblast-like cells adhesion, FN reorganisation and late matrix formation on the different substrates. Even if initial cell adhesion is excellent for every substrate, the size of the focal adhesion plaques increases as the size of the pits in the nanotopography does. This is correlated to FN reorganisation, which only takes places on the 29 and 45. nm deep pits surfaces, where enhanced late matrix production was also found. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {3T3 Cells, Adhesion, Adsorption, AFM, animal cell, Animals, article, Atomic force microscopy, Bioactivity, Biological activities, biological activity, Casting process, cell adhesion, Cell Line, concentration (parameters), Concentration (process), Concentration of, Deep pit, Different substrates, Electric currents, extracellular matrix, Fibronectin, Fibronectin adsorption, Fibronectins, focal adhesion, Focal adhesion plaques, Focal adhesions, Humans, Image analysis, Image Processing, Computer-Assisted, isotope labeling, Landforms, matrix, Matrix formation, Mice, Microscopy, Atomic Force, nanomaterial, Nanoscale topography, Nanotechnology, Nanotopographies, Nanotopography, nonhuman, osteoblast, Osteoblast-like cells, Osteoblasts, Phase separation, polylactic acid, polystyrene, priority journal, Protein adsorption, Protein layers, protein localization, Proteins, Radiolabelling, Reorganisation, Substrates, Surface Properties},
	isbn = {09277765 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77950863692\&partnerID=40\&md5=7fe3bdcc0f69cb64fb3ba7e86fe7a80a},
	author = {Gonz{\'a}lez-Garc{\'\i}a, C. and Sousa, S.R. and Moratal, D. and Rico, P. and Salmer{\'o}n-S{\'a}nchez, M.}
}
@article {2269,
	title = {Heparinized hydroxyapatite/collagen three-dimensional scaffolds for tissue engineering},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {21},
	year = {2010},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-010-4097-2PubMed ID: 20596760Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: collagen, 9007-34-5; cyanamide, 151-51-9, 420-04-2; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; hydroxyapatite, 1306-06-5, 51198-94-8; n hydroxysuccinimide, 6066-82-6; trinitrobenzenesulfonic acid, 16655-63-3, 2508-19-2; Biocompatible Materials; Bone Substitutes; Collagen, 9007-34-5; Cross-Linking Reagents; Durapatite, 1306-06-5; Heparin, 9005-49-6; PolymersReferences: Rose, F.R., Cyster, L.A., Grant, D.M., Scotchford, C.A., Howdle, S.M., Shakesheff, K.M., In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel (2004) Biomaterials, 25 (24), pp. 5507-5514. , DOI 10.1016/j.biomaterials.2004.01.012, PII S0142961204000134;Hong, Z., Zhang, P., He, C., Qiu, X., Liu, A., Chen, L., Chen, X., Jing, X., Nano-composite of poly(L-lactide) and surface grafted hydroxyapatite: Mechanical properties and biocompatibility (2005) Biomaterials, 26 (32), pp. 6296-6304. , DOI 10.1016/j.biomaterials.2005.04.018, PII S0142961205003297; 
Ignjatovic, N., Tomic, S., Dakic, M., Miljkovic, M., Plavsic, M., Uskokovic, D., Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials (1999) Biomaterials, 20 (9), pp. 809-816. , DOI 10.1016/S0142-9612(98)00234-8, PII S0142961298002348; 
Kothapalli, C.R., Shaw, M.T., Wei, M., Biodegradable HA-PLA 3-D porous scaffolds: Effect of nano-sized filler content on scaffold properties (2005) Acta Biomaterialia, 1 (6), pp. 653-662. , DOI 10.1016/j.actbio.2005.06.005, PII S1742706105000978; 
Liao, S., Wang, W., Uo, M., Ohkawa, S., Akasaka, T., Tamura, K., Cui, F., Watari, F., A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration (2005) Biomaterials, 26 (36), pp. 7564-7571. , DOI 10.1016/j.biomaterials.2005.05.050, PII S0142961205004199; 
Maeda, H., Kasuga, T., Hench, L.L., Preparation of poly(L-lactic acid)-polysiloxane-calcium carbonate hybrid membranes for guided bone regeneration (2006) Biomaterials, 27 (8), pp. 1216-1222. , DOI 10.1016/j.biomaterials.2005.08.010, PII S0142961205007829; 
Kong, L., Gao, Y., Cao, W., Gong, Y., Zhao, N., Zhang, X., Preparation and characterization of nano-hydroxyapatite/chitosan composite scaffolds (2005) Journal of Biomedical Materials Research - Part A, 75 (2), pp. 275-282. , DOI 10.1002/jbm.a.30414; 
Li, X.M., Feng, Q.L., Cui, F.Z., In vitro degradation of porous nano-hydroxyapatite/collagen/PLLA scaffold reinforced by chitin fibres (2006) Mater Sci Eng C-Biomim Supramol Syst, 26 (4), pp. 716-720. , 1:CAS:528:DC\%2BD28XjvFyntr4\%3D; 
Sachlos, E., Gotora, D., Czernuszka, J.T., Collagen scaffolds reinforced with biomimetic composite nano-sized carbonate-substituted hydroxyapatite crystals and shaped by rapid prototyping to contain internal microchannels (2006) Tissue Engineering, 12 (9), pp. 2479-2487. , DOI 10.1089/ten.2006.12.2479; 
Zhu, X., Eibl, O., Scheideler, L., Geis-Gerstorfer, J., Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors (2006) Journal of Biomedical Materials Research - Part A, 79 (1), pp. 114-127. , DOI 10.1002/jbm.a.30706; 
Pieper, J.S., Hafmans, T., Veerkamp, J.H., Van Kuppevelt, T.H., Development of tailor-made collagen-glycosaminoglycan matrices: EDC/NHS crosslinking, and ultrastructural aspects (2000) Biomaterials, 21 (6), pp. 581-593. , DOI 10.1016/S0142-9612(99)00222-7, PII S0142961299002227; 
Pieper, J.S., Oosterhof, A., Dijkstra, P.J., Veerkamp, J.H., Van Kuppevelt, T.H., Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate (1999) Biomaterials, 20 (9), pp. 847-858. , DOI 10.1016/S0142-9612(98)00240-3, PII S0142961298002403; 
Pieper, J.S., Van Wachem, P.B., Van Luyn, M.J.A., Brouwer, L.A., Hafmans, T., Veerkamp, J.H., Van Kuppevelt, T.H., Attachment of glycosaminoglycans to collagenous matrices modulates the tissue response in rats (2000) Biomaterials, 21 (16), pp. 1689-1699. , DOI 10.1016/S0142-9612(00)00052-1, PII S0142961200000521; 
Pieper, J.S., Hafmans, T., Van Wachem, P.B., Van Luyn, M.J.A., Brouwer, L.A., Veerkamp, J.H., Van Kuppevelt, T.H., Loading of collagen-heparan sulfate matrices with bFGF promotes angiogenesis and tissue generation in rats (2002) Journal of Biomedical Materials Research, 62 (2), pp. 185-194. , DOI 10.1002/jbm.10267; 
Wissink, M.J.B., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H.M., Beugeling, T., Immobilization of heparin to EDC/NHS-crosslinked collagen. Characterization and in vitro evaluation (2001) Biomaterials, 22 (2), pp. 151-163. , 10.1016/S0142-9612(00)00164-2 1:CAS:528:DC\%2BD3cXovFGnu7g\%3D 11101159; 
Wissink, M.J.B., Beernink, R., Poot, A.A., Engbers, G.H.M., Beugeling, T., Van Aken, W.G., Feijen, J., Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices (2000) Journal of Controlled Release, 64 (1-3), pp. 103-114. , DOI 10.1016/S0168-3659(99)00145-5, PII S0168365999001455; 
Wissink, M.J.B., Beernink, R., Scharenborg, N.M., Poot, A.A., Engbers, G.H.M., Beugeling, T., Van Aken, W.G., Feijen, J., Endothelial cell seeding of (heparinized) collagen matrices: Effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors (2000) Journal of Controlled Release, 67 (2-3), pp. 141-155. , DOI 10.1016/S0168-3659(00)00202-9, PII S0168365900002029; 
Gcm, S., Yao, C., Prevel, P., Markowicz, M., Schenck, P., Noah, E.M., Modulation of angiogenic potential of collagen matrices by covalent incorporation of heparin and loading with vascular endothelial growth factor (2004) Tissue Eng, pp. 1502-9; 
Yao, C., Markowicz, M., Pallua, N., Magnus Noah, E., Steffens, G., The effect of cross-linking of collagen matrices on their angiogenic capability (2008) Biomaterials, 29 (1), pp. 66-74. , DOI 10.1016/j.biomaterials.2007.08.049, PII S0142961207007284; 
Jiao, Y.Y., Ubrich, N., Hoffart, V., Marchand-Arvier, M., Vigneron, C., Hoffman, M., Preparation and characterization of heparin-loaded polymeric microparticles (2002) Drug Dev Ind Pharm, 28 (8), pp. 1033-1041. , 10.1081/DDC-120014740 1:CAS:528:DC\%2BD38XnslGnt7c\%3D 12378958; 
Jiao, Y., Ubrich, N., Marchand-Arvier, M., Vigneron, C., Hoffman, M., Lecompte, T., Maincent, P., In vitro and in vivo evaluation of oral heparin-loaded polymeric nanoparticles in rabbits (2002) Circulation, 105 (2), pp. 230-235. , DOI 10.1161/hc0202.101988; 
Lee, K.W., Yoon, J.J., Lee, J.H., Kim, S.Y., Jung, H.J., Kim, S.J., Joh, J.W., Lee, S.K., Sustained release of vascular endothelial growth factor from calcium-induced alginate hydrogels reinforced by heparin and chitosan (2004) Transplantation Proceedings, 36 (8), pp. 2464-2465. , DOI 10.1016/j.transproceed.2004.08.078, PII S0041134504009480; 
Schroeder-Tefft, J.A., Bentz, H., Estridge, T.D., Collagen and heparin matrices for growth factor delivery (1997) Journal of Controlled Release, 48 (1), pp. 29-33. , DOI 10.1016/S0168-3659(97)00055-2, PII S0168365997000552; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci, 19 (2), pp. 855-859. , 1:CAS:528:DC\%2BD1cXhs1Wktb8\%3D; 
Edlund, U., Danmark, S., Albertsson, A.-C., A strategy for the covalent functionalization of resorbable polymers with heparin and osteoinductive growth factor (2008) Biomacromolecules, 9 (3), pp. 901-905. , DOI 10.1021/bm701267u; 
Teixeira, S., Oliveira, S., Ferraz, M., Monteiro, F.J., Three dimensional macroporous calcium phosphates scaffolds for bone tissue engineering (2008) Key Eng Mater Trans Tech, pp. 947-50; 
Yamamoto, M., Tabata, Y., Hong, L., Miyamoto, S., Hashimoto, N., Ikada, Y., Bone regeneration by transforming growth factor β1 released from a biodegradable hydrogel (2000) Journal of Controlled Release, 64 (1-3), pp. 133-142. , DOI 10.1016/S0168-3659(99)00129-7, PII S0168365999001297; 
Teixeira, S., Rodriguez, M.A., Pena, P., De Aza, A.H., De Aza, S., Ferraz, M.P., Monteiro, F.J., Physical characterization of hydroxyapatite porous scaffolds for tissue engineering (2009) Mater Sci Eng C, 29 (5), pp. 1510-1514. , 10.1016/j.msec.2008.09.052 1:CAS:528:DC\%2BD1MXmtVarurg\%3D
},
	month = {2010///},
	pages = {2385 - 2392},
	abstract = {Currently, in bone tissue engineering research, the development of appropriate biomaterials for the regeneration of bony tissues is a major concern. Bone tissue is composed of a structural protein, collagen type I, on which calcium phosphate crystals are enclosed. For tissue engineering, one of the most applied strategies consists on the development and application of three dimensional porous scaffolds with similar composition to the bone. In this way, they can provide a physical support for cell attachment, proliferation, nutrient transport and new bone tissue infiltration. Hydroxyapatite is a calcium phosphate with a similar composition of bone and widely applied in several medical/dentistry fields. Therefore, in this study, hydroxyapatite three dimensional porous scaffolds were produced using the polymer replication method. Next, the porous scaffolds were homogeneously coated with a film of collagen type I by applying vacuum force. Yet, due to collagen degradability properties, it was necessary to perform an adequate crosslinking method. As a result, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was employed as an efficient and non-toxic crosslinking method in this research. The composites were characterized by means of SEM, DSC and TNBS. Furthermore, heparin was incorporated in order to accomplish sustained delivery of a growth factor of interest namely, bone morphogenetic proteins (BMP-2). BMP-2 binding and release of non-heparinized and heparinized scaffolds was evaluated at specific time points. The incorporation of heparin leads to a reduced initial burst phase when compared to the non heparinized materials. The results show a beneficial effect with the incorporation of heparin and its potential as a localized drug delivery system for the sustained release of growth factors. {\textcopyright} 2010 Springer Science+Business Media, LLC.},
	keywords = {Achilles Tendon, Animals, Apatite, article, Beneficial effects, Binding and release, Biocompatible Materials, Biological materials, Biomaterials, Bone, Bone and Bones, bone morphogenetic protein 2, Bone morphogenetic proteins, Bone Substitutes, bone tissue, Bone tissue engineering, Bony tissue, Calcium, calcium phosphate, Calcium phosphate crystals, Cattle, Cell attachments, Collagen, collagen degradation, collagen type 1, Collagen type I, controlled study, cross linking, Cross-Linking Reagents, Crosslinking, cyanamide, Degradability, differential scanning calorimetry, Drug delivery, drug delivery system, Durapatite, Grafts, Growth factor, heparin, Hydrogels, hydroxyapatite, Microscopy, Electron, Scanning, n (3 dimethyllaminopropyl) n{\textquoteright} ethylcarbodiimide, n hydroxysuccinimide, N-hydroxysuccinimide, Nutrient transport, Peptides, polymer, Polymer replication, Polymers, Polysaccharides, porosity, Porous scaffold, priority journal, protein binding, Scaffolds, Scanning electron microscopy, SEM, Specific time, Structural proteins, Sustained release, Three dimensional, Three-dimensional porous scaffolds, Three-dimensional scaffolds, Tissue engineering, tissue scaffold, Tissue Scaffolds, trinitrobenzenesulfonic acid, unclassified drug, vacuum, Vacuum force},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956420058\&partnerID=40\&md5=a723fdb7ea856a5286080a1a4aab82a2},
	author = {Teixeira, S. and Yang, L. and Dijkstra, P.J. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2273,
	title = {In vitro study of the proliferation and growth of human bone marrow cells on apatite-wollastonite-2M glass ceramics},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {6},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.12.027Language of Original Document: EnglishCorrespondence Address: De Aza, A. H.; Instituto de Cer{\'a}mica y Vidrio, CSIC, C/Kelsen 5, 28049 Madrid, Spain; email: aaza@icv.csic.esChemicals/CAS: apatite, 64476-38-6; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium silicate, 1344-95-2, 13983-17-0; silicate, 12627-13-3; Apatites; Bone Substitutes; Calcium Compounds; Ceramics; Silicates; calcium silicate, 1344-95-2References: Hench, L.L., Wilson, J., (1993) An Introduction to Bioceramics, , London: World Scientific;Hench, L.L., Bioceramics: From concept to clinic (1991) J. Am. Ceram. Soc., 74, pp. 1487-1510; 
Best, S.M., Porter, A.E., Thian, E.S., Huan, J., Bioceramics: Past, present and for the future (2008) J. Eur. Ceram. Soc., 28, pp. 1319-1327; 
Chevalier, J., Gremillard, L., Ceramics for medical applications: A picture for the next 20 years (2009) J. Eur. Ceram. Soc., 29, pp. 1245-1255; 
Teixeira, S., Oliveira, S., Ferraz, M.P., Monteiro, F.J., Mesenchymal stem cell proliferation and morphological analysis on interconnected macroporous structures (2007) Key Eng. Mater., 330-332, pp. 1129-1132; 
Temenoff, J.S., Mikos, A.G., (2008) Biomaterials: The Intersection of Biology and Materials Science, p. 471. , London: Pearson International; 
Carlisle, E.M., Biochemical and morphological changes associated with long bone abnormalities in silicon deficiency (1980) J. Nutr., 110, pp. 1046-1055; 
Carlisle, E.M., A silicon requirement for normal skull formation (1980) J. Nutr., 110, pp. 352-359; 
Reffitt, M., Ogston, N., Jugdaohsingh, R., Cheung, H.F., Evans, B.A., Thompson, R.P., Orthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro (2003) Bone, 32, pp. 127-135; 
Brown, L., Luklinska, Z., De Aza, P.N., De Aza, S., Anseau, M., Hughes, F.J., Mechanism of osteoinduction by pseudowollastonite (psW) ceramic (2004) 7th World Biomaterials Congress, , Paper presented at the, Sydney; 
De Aza, P.N., De Aza, A.H., De Aza, S., Crystalline bioceramic materials (2005) Bol Soc. Esp. Ceram. V, 44, pp. 135-145; 
De Aza, P.N., De Aza, A.H., Pena, P., De Aza, S., Bioactive glasses and glass-ceramics (2007) Bol Soc. Esp. Ceram. V, 46, pp. 45-55; 
Kokubo, T., Ito, S., Sakka, S., Formation of a high-strength bioactive glass-ceramic in the system MgO-CaO-SiO 2-P 2O 5 (1986) J. Mat. Sci., 21, pp. 536-540; 
De Aza, P.N., Guiti{\'a}n, F., De Aza, S., Bioeutectic: A new ceramic material for human bone replacement (1997) Biomaterials, 18, pp. 1285-1291; 
De Aza, P.N., Guiti{\'a}n, F., De Aza, S., A new bioactive material which transforms in situ into hydroxyapatite (1998) Act Mater., 46, pp. 2541-2549; 
De Aza, P.N., Guiti{\'a}n, F., De Aza, S., Bioeutectics (1994) Internacional Trademark, 780 (1), p. 697. , Universidad de Santiago de Compostela; 
De Aza, P.N., Guiti{\'a}n, F., De Aza, S., Bioeutectics: New bioceramic materials (1999) Advances in Science and Technology, 28, pp. 25-32. , Vicenzini P, editor, Materials in clinical applications, Faenza: Publi Techna Italia; 
De Aza, P.N., Luklinska, Z.B., Anseau, M.R., Guitian, F., De Aza, S., Reactivity of wollastonite-tricalcium phosphate ceramic (Bioeutectic {\textregistered}) in parotid human saliva (2000) Biomaterials, 21, pp. 1735-1741; 
De Aza, A.H., Velasquez, P., Alemany, M.I., Pena, P., De Aza, P.N., In situ bone-like apatite formation from a Bioeutectic ceramic in SBF dynamic flow (2007) J. Am. Ceram. Soc., 90, pp. 1200-1207; 
S{\'a}nchez, F.M., D{\'\i}az-G{\"u}emes, I., Enciso, S., Moreno, B., De Aza, A.H., Carrodeguas, R.G., Evaluaci{\'o}n de una cer{\'a}mica bioactiva de p-wollastonita como sustituto {\'o}seo en el modelo ovino (2009) XVII Congreso Internacional de la Sociedad Espa{\~n}ola de Cirug{\'\i}a Veterinaria, , C{\'a}ceres; 6-8 November, Paper Presented at the; 
Llorca, J., Orera, V., Directionally solidified eutectic ceramic oxides (2006) Prog. Mater. Sci., 51, pp. 711-809; 
Magallanes-Perdomo, M., Carrodeguas, R.G., Pena, P., De Aza, P.N., De Aza, S., De Aza, A.H., Non-isothermal devitrification study of wollastonite-tricalcium phosphate Bioeutectic {\textregistered} glass (2009) Key Eng. Mat., 396-398, pp. 127-130; 
Magallanes-Perdomo, M., Pena, P., De Aza, P.N., Carrodeguas, R.G., Rodriguez, M.A., Turrillas, X., Devitrification studies of wollastonite-tricalcium phosphate eutectic glass (2009) Acta Biomater, 5, pp. 3057-3066; 
De Aza, P.N., Guiti{\'a}n, F., De Aza, S., Phase diagram of wollastonite-tricalcium phosphate (1995) J. Am. Ceram. Soc., 78, pp. 1653-1656; 
Young, R.A., (1993) The Rietveld Method, p. 336. , Oxford: Oxford University Press; 
Larson, A.C., Von Dreele, R.B., (1994) Los Alamos National Laboratory Report No. LA-UR-86-748, , Los Alamos, NM: Los Alamos National Laboratory; 
Finger, L.W., Cox, D.E., Jephcoat, A.P., A correction for powder diffraction peak asymmetry due to axial divergence (1994) J. Appl. Cryst., 27, pp. 892-900; 
Wilson, R.M., Elliott, J.C., Dowker, S.E., Rodriguez-Lorenzo, L.M., Rietveld refinements and spectroscopic studies of the structure of Ca-deficient apatite (2005) Biomaterials, 26, pp. 1317-1327; 
Wilson, R.M., Elliott, J.C., Dowker, S.E., Rodriguez-Lorenzo, L.M., Ca 9.303 (PO 4) 6 (OH) 0.6061.97 (H 2O) (2005) ICSD Collection Code 152190, Inorganic Crystal Structure Database, , Karlsruhe: Fachonformationszentrum; 
Trojer, F.J., The crystal structure of parawollastonite (1968) Zeitschrift fur Kristallographie Kristallgeometrie Kristallphysik Kristallchemie, 127, pp. 291-308; 
Trojer, F.J., CaSiO 3-wollastonite-2M (1968) ICSD Collection Code 34908, Inorganic Crystal Structure Database, , Karlsruhe: Fachonformationszentrum; 
De La, T.A.G., Bruque, S., Aranda, M.A.G., Rietveld quantitative amorphous content analysis (2001) J. Appl. Cryst., 34, pp. 196-202; 
De Aza, A.H., De La Torre, A.G., Aranda, M.A.G., Valle, F.J., De Aza, S., Rietveld quantitative analysis of Buen Retiro porcelains (2004) J. Am. Ceram. Soc., 87, pp. 449-454; 
Magallanes-Perdomo, M., Luklinska, Z.B., De Aza, A.H., Carrodeguas, R.G., De Aza, S., Pena, P., Bone like apatite forming ability of apatite-wollastonite glass ceramic (2009) 11th International Conference and Exhibition of the European Ceramic Society, , Krakow, Poland; 21-25 June, Paper Presented at the; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., HA and double-layer HA-P 2O 3/CaO glass coatings: Influence of chemical composition on human bone marrow cells osteoblastic behavior (2001) J. Mater. Sci. Mater. Med., 12, pp. 629-638; 
Meseguer-Olmo, L., Bernabeu-Esclapez, A., Ros-Martinez, E., Sanchez-Salcedo, S., Padilla, S., Martin, A.I., In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO 2-CaO-P 2O 5 system (2008) Acta Biomater, 4, pp. 1104-1113; 
Muller, P., Bulnheim, U., Diener, A., Luthen, F., Teller, M., Klinkenberg, E.D., Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells (2008) J. Cell. Mol. Med., 12, pp. 281-291; 
Saldana, L., Sanchez-Salcedo, S., Izquierdo-Barba, I., Bensiamar, F., Munuera, L., Vallet-Regi, M., Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells (2009) Acta Biomater, 5, pp. 1294-1305; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Effect of titanium surface-roughness on proliferation, differentiation, and protein-synthesis of human osteoblast-like cells (MG63) (1995) J. Biomed. Mater. Res., 29, pp. 389-401; 
Bowers, K.T., Keller, J.C., Randolph, B.A., Wick, D.G., Michaels, C.M., Optimization of surface micromorphology for enhanced osteoblast responses in vitro (1992) Int. J. Oral. Maxillofac. Implants, 7, pp. 302-310; 
Boyan, B.D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D.L., Szmuckler-Moncler, S., Titanium surface roughness alters responsiveness of MG63 osteoblastlike cells to 1 alpha, 25-(OH) (2) D-3 (1998) J. Biomed. Mater. Res., 39, pp. 77-85; 
Mateus, A.Y.P., Barrias, C.C., Ribeiro, C., Ferraz, M.P., Monteiro, F.J., Comparative study of nanohydroxyapatite microspheres for medical applications (2008) J. Biomed. Mater. Res. A, 86, pp. 483-493; 
Ni, S.Y., Chang, J., Chou, L., Zhai, W.Y., Comparison of osteoblast-like cell responses to calcium silicate and tricalcium phosphate ceramics in vitro (2007) J. Biomed. Mater. Res. B. Appl. Biomater, 80, pp. 174-183; 
Sarmento, C., Luklinska, Z.B., Brown, L., Anseau, M., De Aza, P.N., De Aza, S., In vitro behavior of osteoblastic cells cultured in the presence of pseudowollastonite ceramic (2004) J. Biomed. Mater. Res. A, 69 A, pp. 351-358
},
	month = {2010///},
	pages = {2254 - 2263},
	abstract = {This study concerns the preparation and in vitro characterization of an apatite-wollastonite-2M bioactive glass ceramic which is intended to be used for the regeneration of hard tissue (i.e. in dental and craniomaxillofacial surgery). This bioglass ceramic has been obtained by appropriate thermal treatment through the devitrification (crystallization) of a glass with a stoichiometric eutectic composition within the Ca                        3(PO 
                        4)2-CaSiO
                        3 binary system. Crack-free specimens of the bioglass ceramic were immersed in human bone marrow cell cultures for 3, 7, 14 and 21 days, in order to study biocompatibility. Cell morphology, proliferation and colonization were assessed by scanning electron microscopy and confocal laser scanning microscopy. A total protein content assay was used to evaluate the viability and proliferation of cultured bone marrow cells. The results showed that the cells were able to adhere and proliferate on the designed material due to the essentiality of silicon and calcium as accessory factors for cell activity stimulation. {\textcopyright} 2009 Acta Materialia Inc. All rights reserved.
},
	keywords = {Apatite, Apatites, article, Bioceramics, Biocompatibility, bone marrow cell, bone prosthesis, Bone Substitutes, Calcium Compounds, calcium derivative, calcium phosphate, calcium silicate, cell activity, cell adhesion, Cell culture, cell differentiation, cell enlargement, cell growth, cell proliferation, cell structure, cell viability, Cells, Cultured, ceramics, chemistry, confocal laser microscopy, crystal structure, Crystallization, cytology, glass, Glass ceramics, human, human cell, Humans, in vitro study, materials testing, Mesenchymal stem cell, Mesenchymal Stem Cells, methodology, osteoblast, Osteoblasts, physiology, priority journal, protein content, Scanning electron microscopy, silicate, Silicates, Surface Properties, surface property, Tissue regeneration, Wollastonite, X ray fluorescence},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956625645\&partnerID=40\&md5=426d361faf4a2e3f988754fc5822ea56},
	author = {Magallanes-Perdomo, M. and De Aza, A.H. and Mateus, A.Y. and Teixeira, S. and Monteiro, F.J. and De Aza, S. and Pena, P.}
}
@article {2276,
	title = {In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {93},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32532PubMed ID: 19591232Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Ceramics; Durapatite, 1306-06-5References: Jones, A.C., Milthorpe, B., Averdunk, H., Limaye, A., Senden, T.J., Sakellariou, A., Sheppard, A.P., Dwdw, H., Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging (2004) Biomaterials, 25, pp. 4947-4954;Aizawa, M., Shinoda, H., Uchida, H., Itatani, K., Okada, I., Matsumoto, M., Toyama, Y., Development and biological evaluation of apatite fibre scaffolds with large pore size and high porosity for bone regeneration (2003) Bioceramics, 240-242, pp. 647-650; 
Ebaretonbofa, E., Evans, J.R.G., High porosity hydroxyapatite foam scaffolds for bone substitute (2002) J Porous Mater, 9, pp. 257-263; 
Wissink, M.J.B., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H.M., Beugeling, T., Van Aken, W.G., Feijen, J., Immobilization of heparin to EDC/NHS-crosslinked collagen (2001) Characterization and in Vitro Evaluation. Biomaterials, 22, pp. 151-163; 
Lee, C.H., Singla, A., Lee, Y., Biomedical applications of collagen (2001) Int J Pharm, 221, pp. 1-22; 
Jensen, T.B., Overgaard, S., Lind, M., Rahbek, O., B{\"u}nger, C., S{\o}balle, K., Osteogenic protein 1 device increases bone formation and bone graft resorption around cementless implants (2002) Acta Orthop Scand, 73, pp. 31-39; 
Niemeyer, P., Krause, U., Fellenberg, J., Kasten, P., Seckinger, A., Ho, A.D., Simank, H.G., Evaluation of mineralized collagen and alpha-tricalcium phosphate as scaffolds for tissue engineering of bone using human mesenchymal stem cells (2004) Cells Tissues Organs, 177, pp. 68-78; 
Yang, X.B., Bhatnagar, R.S., Li, S., Oreffo, R.O., Biomimetic collagen scaffolds for human bone cell growth and differentiation (2004) Tissue Eng, 10, pp. 1148-1159; 
Xu, C., Wang, Y., Yu, X., Chen, X., Li, X., Yang, X., Li, S., Xiang, A.P., Evaluation of human mesenchymal stem cells response to biomimetic bioglass-collagen-hyaluronic acidphosphatidylserine composite scaffolds for bone tissue engineering (2009) J Biomed Mater Res A, 88, pp. 264-273; 
Kikuchi, M., Matsumoto, H.N., Yamada, T., Koyama, Y., Takakuda, K., Tanaka, J., Glutaraldehyde cross-linked hydroxyapatite/collagen self-organized nanocomposites (2004) Biomaterials, 25, pp. 63-69; 
Liao, S., Wang, W., Uo, M., Ohkawa, S., Akasaka, T., Tamura, K., Cui, F., Watari, F., A three-layered nano-carbonated hydroxyapatite/ collagen/PLGA composite membrane for guided tissue regeneration (2005) Biomaterials, 26, pp. 7564-7571; 
Liao, S., Watari, F., Zhu, Y., Uo, M., Akasaka, T., Wang, W., Xu, G., Cui, F., The degradation of the three layered nano-carbonated hydroxyapatite/ collagen/PLGA composite membrane in vitro (2007) Dent Mater, 23, pp. 1120-1128; 
Zhang, S.M., Cui, F.Z., Liao, S.S., Zhu, Y., Han, L., Synthesis and biocompatibility of porous nano-hydroxyapatite/collagen/ alginate composite (2003) J Mater Sci, 14, pp. 641-645; 
Glowacki, J., Mizuno, S., Collagen scaffolds for tissue engineering (2008) Biopolymers, 89, pp. 338-344; 
Hao, W., Hu, Y.Y., Wei, Y.Y., Pang, L., Lv, R., Bai, J.P., Xiong, Z., Jiang, M., Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold (2008) Cells Tissues Organs, 187, pp. 89-102; 
Liuyun, J., Yubao, L., Li, Z., Jianguo, L., Preparation and properties of a novel bone repair composite: Nano-hydroxyapatite/ chitosan/carboxymethyl cellulose (2008) J Mater Sci, 19, pp. 981-987; 
Zhou, G., Li, Y., Zhang, L., Zuo, Y., Jansen, J.A., Preparation and characterization of nano-hydroxyapatite/chitosan/konjac glucomannan composite (2007) J Biomed Mater Res, 83, pp. 931-939; 
Hua, Y., Ning, C., Xiaoying, L., Buzhong, Z., Wei, C., Xiaoling, S., Natural hydroxyapatite/chitosan composite for bone substitute materials (2005) Conf Proc IEEE Eng Med Biol Soc, 5, pp. 4888-4891; 
Oliveira, J.M., Rodrigues, M.T., Silva, S.S., Malafaya, P.B., Gomes, M.E., Viegas, C.A., Dias, I.R., Reis, R.L., Novel hydroxyapatite/ chitosan bilayered scaffold for osteochondral tissue- Engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells (2006) Biomaterials, 27, pp. 6123-6137; 
Kong, L., Gao, Y., Cao, W., Gong, Y., Zhao, N., Zhang, X., Preparation and characterization of nano-hydroxyapatite/chitosan composite scaffolds (2005) J Biomed Mater Res, 75, pp. 275-282; 
Zhao, F., Yin, Y., Lu, W.W., Leong, J.C., Zhang, W., Zhang, J., Zhang, M., Yao, K., Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds (2002) Biomaterials, 23, pp. 3227-3234; 
Everaerts, F., Torrianni, M., Hendriks, M., Feijen, J., Biomechanical properties of carbodiimide crosslinked collagen: Influence of the formation of ester crosslinks (2008) J Biomed Mater Res A, 85, pp. 547-555; 
Vrana, N.E., Builles, N., Kocak, H., Gulay, P., Justin, V., Malbouyres, M., Ruggiero, F., Hasirci, V., EDC/NHS cross-linked collagen foams as scaffolds for arti.cial corneal stroma (2007) J Biomater Sci, 18, pp. 1527-1545; 
Wissink, M.J.B., Beernink, R., Scharenborg, N.M., Poot, A.A., Engbers, G.H.M., Beugeling, T., Van Aken, W.G., Feijen, J., Endothelial cell seeding of (heparinized) collagen matrices: Effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors (2000) J Control Release, 67, pp. 141-155; 
Wissink, M.J.B., Van Luyn, M.J.A., Beernink, R., Dijk, F., Poot, A.A., Engbers, G.H.M., Beugeling, T., Feijen, J., Endothelial cell seeding on crosslinked collagen: Effects of crosslinking on endothelial cell proliferation and functional parameters (2000) Thrombosis Haemostasis, 84, pp. 325-331; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci Mater Med, 19, pp. 855-859; 
Wissink, M.J.B., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H.M., Beugeling, T., Van Aken, W.G., Feijen, J., Binding and release of basic fibroblast growth factor from heparinized collagen matrices (2001) Biomaterials, 22, pp. 2291-2299; 
Wissink, M.J.B., Beernink, R., Poot, A.A., Engbers, G.H.M., Beugeling, T., Van Aken, W.G., Feijen, J., Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices (2000) J Control Release, 64, pp. 103-114; 
Habibovic, P., Yuan, H., Van Den Doel, M.A., Sees, T.M., Van Blitterswijk, C.A., De Groot, K., Relevance of osteoinductive biomaterials in critical-sized orthotopic defect (2006) J Orthop Res, 24, pp. 867-876; 
Habibovic, P., Sees, T.M., Van Den Doel, M.A., Van Blitterswijk, C.A., De Groot, K., Osteoinduction by biomaterials-physicochemical and structural influences (2006) J Biomed Mater Res A, 77, pp. 747-762; 
Both, S.K., Van Der Muijsenberg, A.J., Van Blitterswijk, C.A., De Boer, J., De Bruijn, J.D., A rapid and ef.cient method for expansion of human mesenchymal stem cells (2007) Tissue Eng, 13, pp. 3-9; 
Habibovic, P., Gbureck, U., Doillon, C.J., Bassett, D.C., Van Blitterswijk, C.A., Barralet, J.E., Osteoconduction and osteoinduction of low-temperature 3D printed bioceramic implants (2008) Biomaterials, 29, pp. 944-953; 
Teixeira, S., Oliveira, S., Ferraz, M.P., Monteiro, F.J., Three dimensional macroporous calcium phosphate scaffolds for bone tissue engineering (2008) Key Eng Mater, 361-363 (2), pp. 947-950; 
Teixeira, S., Rodriguez, M.A., Pena, P., De Aza, A.H., De Aza, S., Ferraz, M.P., Monteiro, F.J., Physical characterization of hydroxyapatite porous scaffolds for tissue engineering (2009) Mat Sci Eng: C, 29, pp. 1510-1514; 
Kim, H.W., Knowles, J.C., Kim, H.E., Hydroxyapatite porous scaffold engineered with biological polymer hybrid coating for antibiotic Vancomycin release (2005) J Mater Sci, 16, pp. 189-195; 
Gibson, I.R., Best, S.M., Bonfield, W., Chemical characterization of silicon-substituted hydroxyapatite (1999) J Biomed Mater Res, 44, pp. 422-428; 
Siddappa, R., Fernandes, H., Liu, J., Van Blitterswijk, C., De Boer, J., The response of human mesenchymal stem cells to osteogenic signals and its impact on bone tissue engineering (2007) Curr Stem Cell Res Ther, 2, pp. 209-220; 
Olivo, C., Alblas, J., Verweij, V., Van Zonneveld, A.J., Dhert, W.J., Martens, A.C., In vivo bioluminescence imaging study to monitor ectopic bone formation by luciferase gene marked mesenchymal stem cells (2008) J Orthop Res, 26, pp. 901-909; 
Yuan, H., Van Blitterswijk, C.A., De Groot, K., De Bruijn, J.D., A comparison of bone formation in biphasic calcium phosphate (BCP) and hydroxyapatite (HA) implanted in muscle and bone of dogs at different time periods (2006) J Biomed Mater Res A, 78, pp. 139-147; 
Habibovic, P., Li, J., Van Der Valk, C.M., Meijer, G., Layrolle, P., Van Blitterswijk, C.A., Groot, K., Biological performance of uncoated and octacalcium phosphate-coated Ti6Al4V (2005) Biomaterials, 26, pp. 23-36; 
Habibovic, P., Yuan, H., Van Der Valk, C.M., Meijer, G., Van Blitterswijk, C.A., De Groot, K., 3D microenvironment as essential element for osteoinduction by biomaterials (2005) Biomaterials, 26, pp. 3565-3575
},
	month = {2010///},
	pages = {567 - 575},
	abstract = {During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA based porous scaffolds with collagen type I. The results indicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds. {\textcopyright} 2009 Wiley Periodicals, Inc.},
	keywords = {animal cell, animal experiment, animal model, Animals, article, Biocompatibility, Biocompatible Materials, Biodegradation, Bone, Bone and Bones, Bone formation, bone tissue, Calcium, Calcium alloys, calcium phosphate, Calcium phosphates, Cell Count, Cell culture, Cell Culture Techniques, Cells, Cultured, cellular distribution, Ceramic materials, ceramics, Collagen, collagen type 1, Durapatite, Energy dispersive spectroscopy, Flowcharting, Fourier transform infrared spectroscopy, Humans, hydroxyapatite, Hydroxylation, in vitro study, In vivo bone formation, in vivo study, In-vivo, infrared spectroscopy, Macroporous, Macroporous scaffolds, male, materials testing, Mesenchymal stem cell, Mesenchymal Stem Cells, Mice, Microscopy, Electron, Scanning, nonhuman, ossification, Osteogenesis, porous polymer, rat, Rats, Rats, Wistar, Scaffolds, Scanning electron microscopy, Spectroscopy, Fourier Transform Infrared, Stem cells, Tissue engineering, tissue scaffold, Tissue Scaffolds},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77950190285\&partnerID=40\&md5=612e26b56020935c7b0b1c564bffa89a},
	author = {Teixeira, S. and Fernandes, H. and Leusink, A. and Van Blitterswijk, C. and Ferraz, M.P. and Monteiro, F.J. and De Boer, J.}
}
@article {2248,
	title = {Influence of crystallite size of nanophased hydroxyapatite on fibronectin and osteonectin adsorption and on MC3T3-E1 osteoblast adhesion and morphology},
	journal = {Journal of Colloid and Interface ScienceJ. Colloid Interface Sci.},
	volume = {351},
	year = {2010},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: JCISAdoi: 10.1016/j.jcis.2010.08.013PubMed ID: 20810127Language of Original Document: EnglishCorrespondence Address: Ribeiro, N.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: nribeiro@ineb.up.ptChemicals/CAS: fibronectin, 86088-83-7; hydroxyapatite, 1306-06-5, 51198-94-8; osteonectin, 104052-78-0; protein, 67254-75-5; Ceramics; Durapatite, 1306-06-5; Fibronectins; OsteonectinReferences: Griffith, L.G., Naughton, G., (2002) Science, 295, p. 1009;Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., (2000) J. Biomed. Mater. Res., 51, pp. 475-483; 
Webster, T.J., Siegel, R.W., Bizios, R., (1999) Biomaterials, 20, pp. 1221-1227; 
Jun, I.K., Jang, J.H., Kim, H.W., Kim, H.E., (2005) J. Mater. Sci., 40, pp. 2891-2895; 
Geiger, B., Bershadsky, A., Pankov, R., Yamada, K.M., (2001) Nat. Rev. Mol. Cell Biol., 2, pp. 793-805; 
Globus, R.K., Doty, S.B., Lull, J.C., Holmuhamedov, E., Humphries, M.J., Damsky, C.H., (1998) J. Cell Sci., 111, pp. 1385-1393; 
Moursi, A.M., Damsky, C.H., Lull, J., Zimmerman, D., Doty, S.B., Aota, S.I., Globus, R.K., (1996) J. Cell Sci., 109, pp. 1369-1380; 
Garcia, A.J., Vega, M.D., Boettiger, D., (1999) Mol. Biol. Cell, 10, pp. 785-798; 
Petrie, T.A., Capadona, J.R., Reyes, C.D., Garcia, A.J., (2006) Biomaterials, 27, pp. 5459-5470; 
Dolatshahi-Pirouz, A., Jensen, T., Foss, M., Chevallier, J., Besenbacher, F., (2009) Langmuir, 25, pp. 2971-2978; 
Shen, J.-W., Wu, T., Wang, Q., Pan, H.-H., (2008) Biomaterials, 29, pp. 513-532; 
Brekken, R.A., Sage, E.H., (2000) Matrix Biol., 19, pp. 569-580; 
Termine, J.D., Kleinman, H.K., Whitson, S.W., Conn, K.M., McGarvey, M.L., Martin, G.R., (1981) Cell, 26, pp. 99-105; 
Liao, S., Ngiam, M., Chan, C.K., Ramakrishna, S., (2009) Biomed. Mater., 4; 
Sarvestani, A.S., He, X., Jabbari, E., (2008) Eur. Biophys. J. Biophys. Lett., 37, pp. 229-234; 
Majeska, R.J., Cell Biology of Bone (1934), pp. 2-3. , in: S.C. Cowin (Ed.), Bone Mechanics HandbookKoblinski, J.E., Wu, M., Demeler, B., Jacob, K., Kleinman, H.K., (2005) J. Cell Sci., 118, pp. 2965-2974; 
O{\textquoteright}Brien, J., Wilson, I., Orton, T., Pognan, F., (2000) Eur. J. Biochem., 267, pp. 5421-5426; 
Ribeiro, C.C., Gibson, I., Barbosa, M.A., (2006) Biomaterials, 27, pp. 1749-1761; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., (1999) J. Biomed. Mater. Res., 45, pp. 370-375; 
Suzuki, T., Nishizawa, K., Yokogawa, Y., Nagata, F., Kawamoto, Y., Kameyama, T., (1996) J. Ferment. Bioeng., 81, pp. 226-232; 
Suzuki, T., Yamamoto, T., Toriyama, M., Nishizawa, K., Yokogawa, Y., Mucalo, M.R., Kawamoto, Y., Kameyama, T., (1997) J. Biomed. Mater. Res., 34, pp. 507-517; 
Holland, N.B., Marchant, R.E., (2000) J. Biomed. Mater. Res., 51, pp. 307-315; 
Kothapalli, C., Wei, M., Vasiliev, A., Shaw, M.T., (2004) Acta Mater., 52, pp. 5655-5663; 
Sousa, S.R., Manuela Bras, M., Moradas-Ferreira, P., Barbosa, M.A., (2007) Langmuir, 23, pp. 7046-7054; 
Rouahi, M., Champion, E., Gallet, O., Jada, A., Anselme, K., (2006) Colloids Surf., B - Biointerfaces, 47, pp. 10-19; 
dos Santos, E.A., Farina, M., Soares, G.A., Anselme, K., (2008) J. Mater. Sci. - Mater. Med., 19, pp. 2307-2316; 
Amaral, I.F., Unger, R.E., Fuchs, S., Mendonca, A.M., Sousa, S.R., Barbosa, M.A., Pego, A.P., Kirkpatrick, C.J., (2009) Biomaterials, 30, pp. 5465-5475; 
Sousa, S.R., Moradas-Ferreira, P., Barbosa, M.A., (2005), pp. 1173-1178. , TiO2 type influences fibronectin adsorption, in: 19th Conference of the European-Society-for-Biomaterials, Springer, Sorrento, ItalyGoldstein, A.S., DiMilla, P.A., (2002) J. Biomed. Mater. Res., 59, pp. 665-675; 
Michael, K.E., Vernekar, V.N., Keselowsky, B.G., Meredith, J.C., Latour, R.A., Garcia, A.J., (2003) Langmuir, 19, pp. 8033-8040; 
Anselme, K., Bigerelle, M., (2005) Acta Biomater., 1, pp. 211-222; 
Anselme, K., Bigerelle, M., Noel, B., Dufresne, E., Judas, D., Iost, A., Hardouin, P., (2000) J. Biomed. Mater. Res., 49, pp. 155-166; 
Wang, C., Duan, Y., Markovic, B., Barbara, J., Rolfe Howlett, C., Zhang, X., Zreiqat, H., (2004) Biomaterials, 25, pp. 2949-2956; 
Smith, I.O., McCabe, L.R., Baumann, M.J., (2006) Int. J. Nanomed., 1, pp. 189-194; 
Bernards, M.T., Qin, C.L., Jiang, S.Y., Bone osteopontin, and bovine serum albumin (2008) Colloids Surf., B - Biointerfaces, 64, pp. 236-247; 
Durrieu, M.C., Pallu, S., Guillemot, F., Bareille, R., Amedee, J., Baquey, C., Labrugere, C., Dard, M., (2004) J. Mater. Sci. - Mater. Med., 15, pp. 779-786; 
Itoh, D., Yoneda, S., Kuroda, S., Kondo, H., Umezawa, A., Ohya, K., Ohyama, T., Kasugai, S., (2002) J. Biomed. Mater. Res., 62, pp. 292-298; 
Funk, S.E., Sage, E.H., (1993) J. Cell. Physiol., 154, pp. 53-63; 
Hagan, S., Hiscott, P., Sheridan, C., Wong, D., Grierson, I., McGalliard, J., (2003) Mol. Vis., 9, pp. 87-92; 
Deligianni, D., Korovessis, P., Porte-Derrieu, M.C., Amedee, J., (2005) J. Spinal Disord. Technol., 18, pp. 257-262; 
Nelson, M., Balasundaram, G., Webster, T.J., (2006) Int. J. Nanomed., 1, pp. 339-349
},
	month = {2010///},
	pages = {398 - 406},
	abstract = {The characteristic topographical features (crystallite dimensions, surface morphology and roughness) of bioceramics may influence the adsorption of proteins relevant to bone regeneration. This work aims at analyzing the influence of two distinct nanophased hydroxyapatite (HA) ceramics, HA725 and HA1000 on fibronectin (FN) and osteonectin (ON) adsorption and MC3T3-E1 osteoblast adhesion and morphology. Both substrates were obtained using the same hydroxyapatite nanocrystals aggregates and applying the sintering temperatures of 725{\textdegree}C and 1000{\textdegree}C, respectively. The two proteins used in this work, FN as an adhesive glycoprotein and ON as a counter-adhesive protein, are known to be involved in the early stages of osteogenesis (cell adhesion, mobility and proliferation). The properties of the nanoHA substrates had an important role in the adsorption behavior of the two studied proteins and clearly affected the MC3T3-E1 morphology, distribution and metabolic activity. HA1000 surfaces presenting slightly larger grain size, higher root-mean-square roughness (Rq), lower surface area and porosity, allowed for higher amounts of both proteins adsorbed. These substrates also revealed increased number of exposed FN cell-binding domains as well as higher affinity for osteonectin. Regarding the osteoblast adhesion results, improved viability and cell number were found for HA1000 surfaces as compared to HA725 ones, independently of the presence or type of adsorbed protein. Therefore the osteoblast adhesion and metabolic activity seemed to be more sensitive to surfaces morphology and roughness than to the type of adsorbed proteins. {\textcopyright} 2010 Elsevier Inc.},
	keywords = {Adhesion, Adhesive proteins, Adsorption, Adsorption behavior, Animals, Apatite, article, Bioceramics, Biological materials, Biomaterials, bone development, Bone Regeneration, cell adhesion, Cell Count, cell metabolism, cell motility, Cell number, cell proliferation, cell strain 3T3, cell structure, Cell-binding domain, Cells, Cultured, cellular distribution, ceramics, controlled study, Crystallite dimension, Crystallite size, Crystallization, Durapatite, Fibronectin, Fibronectin adsorption, Fibronectins, glycoprotein, grain, Grain size, human, human cell, hydroxyapatite, Hydroxyapatite nanocrystals, MC3T3-E1, Metabolic activity, metabolism, Mice, morphology, Nano-HA, nanocrystal, nanoparticles, Nanophased hydroxyapatite, osteoblast, Osteoblast adhesion, Osteoblast morphology, Osteoblasts, Osteogenesis, osteonectin, Osteonectin adsorption, Particle Size, porosity, priority journal, protein, Proteins, Root mean square roughness, Sintering, Sintering temperatures, Surface area, Surface Properties, surface property, Surface roughness, temperature, Topographical features},
	isbn = {00219797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77957328472\&partnerID=40\&md5=c4a13ccec89e04dbfe1920fd42fec3f5},
	author = {Ribeiro, N. and Sousa, S.R. and Monteiro, F.J.}
}
@article {2245,
	title = {Innovative macroporous granules of nanostructured-hydroxyapatite agglomerates: Bioactivity and osteoblast-like cell behaviour},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32916PubMed ID: 20845490Language of Original Document: EnglishCorrespondence Address: Laranjeira, M. S.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: dce06004@fe.up.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; colony stimulating factor 1, 81627-83-0; hydroxyapatite, 1306-06-5, 51198-94-8; osteoprotegerin, 205944-50-9; polyurethan, 61789-63-7; Biocompatible Materials; Biological Markers; Durapatite, 1306-06-5; PolyurethanesReferences: Ferraz, M., Monteiro, F., Manuel, C., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80;Murugan, R., Ramakrishna, S., Development of nanocomposites for bone grafting (2005) Compos Sci Technol, 65, pp. 2385-2406; 
Wahl, D., Czernuszka, J.T., Collagen-hydroxyapatite composites for hard tissue repair (2006) Eur Cell Mater, 11, pp. 43-46; 
Chen, Q.Z., Wong, C.T., Lu, W.W., Cheung, K.M., Leong, J.C., Luk, K.D., Strengthening mechanisms of bone bonding to crystalline hydroxyapatite in vivo (2004) Biomaterials, 25, pp. 4243-4254; 
Watari, F., Yokoyama, A., Gelinsky, M., Pompe, W., Conversion of functions by nanosizing from osteoconductivity to bone substitutional properties in apatite (2007) Interface Oral Health Sci, 23, pp. 139-148; 
Nihouannen, D., Duval, L., Lecomte, A., Julien, M., Guicheux, J., Daculsi, G., Layrolle, P., Interactions of total bone marrow cells with increasing quantities of macroporous calcium phosphate ceramic granules (2007) J Mater Sci Mater Med, 18, pp. 1983-1990; 
Le Nihouannen, D., Daculsi, G., Saffarzadeh, A., Gauthier, O., Delplace, S., Pilet, P., Layrolle, P., Ectopic bone formation by microporous calcium phosphate ceramic particles in sheep muscles (2005) Bone, 36, pp. 1086-1093; 
Kalita, S.J., Bhardwaj, A., Bhatt, H.A., Nanocrystalline calcium phosphate ceramics in biomedical engineering (2007) Mater Sci Eng C, 27, pp. 441-449; 
Kong, L.B., Ma, J., Boey, F., Nanosized hydroxyapatite powders derived from coprecipitation process (2002) J Mater Sci, 37, pp. 1131-1134; 
Habibovic, P., Kruyt, M.C., Juhl, M.V., Clyens, S., Martinetti, R., Dolcini, L., Theilgaard, N., Blitterswijk, C., Comparative in vivo study of six hydroxyapatite-based bone graft substitutes (2008) J Orthop Res, 26, pp. 1363-1370; 
Simon, J.L., Michna, S., Lewis, J.A., Rekow, E.D., Thompson, V.P., Smay, J.E., Yampolsky, A., Ricci, J.L., In vivo bone response to 3D periodic hydroxyapatite scaffolds assembled by direct ink writing (2007) J Biomed Mater Res A, 83, pp. 747-758; 
Clarke, S.A., Hoskins, N.L., Jordan, G.R., Henderson, S.A., Marsh, D.R., In vitro testing of advanced JAX TM bone void filler system: Species differences in the response of bone marrow stromal cells to b tri-calcium phosphate and carboxymethylcellulose gel (2007) J Mater Sci Mater Med, 18, pp. 2283-2290; 
Wei, G., Ma, P.X., Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering (2004) Biomaterials, 25, pp. 4749-4757; 
Xu, T., Zhang, N., Nichols, H.L., Shi, D., Wen, X., Modification of nanostructured materials for biomedical applications (2007) Mater Sci Eng C, 27, pp. 579-594; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration (2007) Key Eng Mater, 330-332, pp. 243-246; 
Potoczek, M., Zima, A., Paszkiewicz, Z., Slosarczyk, A., Manufacturing of highly porous calcium phosphate bioceramics via gel-casting using agarose (2009) Ceram Int, 35, pp. 2249-2254; 
Okamoto, M., Dohi, Y., Ohgushi, H., Shimaoka, H., Ikeuchi, M., Matsushima, A., Yonemasu, K., Hosoi, H., Influence of the porosity of hydroxyapatite ceramics on in vitro and in vivo bone formation by cultured rat bone marrow stromal cells (2006) J Mater Sci Mater Med, 17, pp. 327-336; 
Tas, A.C., Preparation of porous apatite granules from calcium phosphate cement (2008) J Mater Sci Mater Med, 19, pp. 2231-2239; 
Joschek, S., Nies, B., Krotz, R., Gokpferich, A., Chemical and physicochemical characterization of porous hydroxyapatite ceramics made of natural bone (2000) Biomaterials, 21, pp. 1645-1658; 
Turhani, D., Cvikl, B., Watzinger, E., Wei{\ss}enb{\"o}ck, M., Yerit, K., Thurnher, D., Lauer, G., Ewers, R., In vitro growth and differentiation of osteoblast-like cells on hydroxyapatite ceramic granule calcified from red algae (2005) J Oral Maxillofac Surg, 63, pp. 793-799; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci Mater Med, 19, pp. 855-859; 
Kim, Z., Oak, J., Kimura, H., Goto, T., Inoue, A., Yoon, S., Architecture of porous hydroxyapatite scaffolds using polymer foam process (2009) J Biomech Sci Eng, 4, pp. 377-383; 
Chevalier, E., Viana, M., Cazalbou, S., Makein, L., Dubois, J., Chulia, D., Ibuprofen-loaded calcium phosphate granules: Combination of innovative characterization methods to relate mechanical strength to drug location (2010) Acta Biomater, 6, pp. 266-274; 
Taboas, J.M., Maddox, R.D., Krebsbach, P.H., Hollister, S.J., Indirect solid free form fabrication of local and global porous, biomimetic and composite 3D polymer-ceramic scaffolds (2003) Biomaterials, 24, pp. 181-194; 
Wang, X., Ruan, J., Chen, Q., Effects of surfactants on the microstructure of porous ceramic scaffolds fabricated by foaming for bone tissue engineering (2009) Mater Res Bull, 44, pp. 1275-1279; 
Mateus, A.Y.P., Barrias, C.C., Ribeiro, C., Ferraz, M.P., Monteiro, F.J., Comparative study of nanohydroxyapatite microspheres for medical applications (2008) J Biomed Mater Res A, 86, pp. 483-493; 
Gibson, I.R., Best, S.M., Bonfield, W., Chemical characterization of silicon-substituted hydroxyapatite (1999) J Biomed Mater Res, 44, pp. 422-428; 
Panda, R.N., Hsieh, M.F., Chung, R.J., Chin, T.S., FTIR, XRD, SEM and solid state NMR investigations of carbonate-containing hydroxyapatite nano-particles synthesized by hydroxide-gel technique (2003) J Phys Chem Solids, 64, pp. 193-199; 
Penel, G., Leroy, G., Rey, C., Sombret, B., Huvenne, J.P., Bres, E., Infrared and Raman microspectrometry study of fluor-fluor-hydroxy and hydroxy-apatite powders (1997) J Mater Sci Mater Med, 8, pp. 271-276; 
Elliott, J.C., Holcomb, D.W., Young, R.A., Infrared determination of the degree of substitution of hydroxyl by carbonate ions in human dental enamel (1985) Calcif Tissue Int, 37, pp. 372-375; 
Rouahi, M., Gallet, O., Champion, E., Dentzer, J., Hardouin, P., Anselme, K., Influence of hydroxyapatite microstructure on human bone cell response (2006) J Biomed Mater Res A, 78, pp. 222-235; 
Fierz, F.C., Beckmann, F., Huser, M., Irsen, S.H., Leukers, B., Witte, F., Degistirici, O., Muller, B., The morphology of anisotropic 3D-printed hydroxyapatite scaffolds (2008) Biomaterials, 29, pp. 3799-3806; 
Singh, R., Lee, P.D., Lindley, T.C., Dashwood, R.J., Ferrie, E., Imwinkelried, T., Characterization of the structure and permeability of titanium foams for spinal fusion devices (2009) Acta Biomater, 5, pp. 477-487; 
Wei, J., Jia, J., Wu, F., Wei, S., Zhou, H., Zhang, H., Shin, J.W., Liu, C., Hierarchically microporous/macroporous scaffold of magnesium-calcium phosphate for bone tissue regeneration (2010) Biomaterials, 31, pp. 1260-1269; 
Rucker, M., Laschke, M.W., Junker, D., Carvalho, C., Tavassol, F., Mulhaupt, R., Gellrich, N.C., Menger, M.D., Vascularization and biocompatibility of scaffolds consisting of different calcium phosphate compounds (2007) J Biomed Mater Res A, 68, pp. 1002-1011; 
Klenke, F.M., Liu, Y., Yuan, H., Hunziker, E.B., Siebenrock, K.A., Hofstetter, W., Impact of pore size on the vascularization and osseointegration of ceramic bone substitutes in vivo (2007) J Biomed Mater Res A, 85, pp. 777-786; 
Lee, S.J., Choi, J.S., Park, K.S., Khang, G., Lee, Y.M., Lee, H.B., Response of MG63 osteoblast-like cells onto polycarbonate membrane surfaces with different micropore sizes (2004) Biomaterials, 25, pp. 4699-4707; 
Kim, H., Lee, E., Kim, H., Salih, V., Knowles, J.C., Effect of fluoridation of hydroxyapatite in hydroxyapatite- polycaprolactone composites on osteoblast activity (2005) Biomaterials, 25, pp. 4395-4404; 
Son, E., Do, H., Joo, H., Pyo, S., Induction of alkaline phosphatase activity by L-ascorbic acid in human osteoblastic cells: A potential role for CK2 and Ikaros (2007) Nutrition, 23, pp. 745-753; 
Guo, X., Gough, J.E., Xiao, P., Liu, J., Shen, Z., Fabrication of nanostructured hydroxyapatite and analysis of human osteoblastic cellular response (2007) J Biomed Mater Res A, 82, pp. 1022-1032; 
Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., Enhanced functions of osteoblasts on nanophase ceramics (2000) Biomaterials, 21, pp. 1803-1810; 
Mendon{\c c}a, G., Mendon{\c c}a, D.B., Sim{\~o}es, L.G., Ara{\'u}jo, A.L., Leite, E.R., Duarte, W.R., Arag{\~a}o, F.J., Cooper, L.F., The effects of implant surface nanoscale features on osteoblast-specific gene expression (2009) Biomaterials, 30, pp. 4053-4062
},
	month = {2010///},
	pages = {891 - 900},
	abstract = {To modulate the biological response of implantable granules, two types of bioactive porous granules composed of nanostructured-hydroxyapatite (HA) agglomerates and microstructured-HA, respectively, were prepared using a polyurethane sponge impregnation and burnout method. The resulting granules presented a highly porous structure with interconnected porosity. Both types of granules were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and mercury intrusion porosimetry. Results showed that nanostructed-HA granules presented higher surface area and porosity than microstructured-HA granules. In vitro testing using MG63 human osteoblast-like cells showed that on both types of surfaces cells were able to adhere, proliferate, and migrate through the macropores, and a higher growth rate was achieved on nanostructured-HA granules than on microstructured-HA granules (76 and 40\%, respectively). In addition, these cells maintained similar expression levels of osteoblastic-associated markers namely collagen type I, alkaline phosphatase, bone morphogenetic protein-2, macrophage colony-stimulating factor, and osteoprotegerin. These innovative nanostructured-HA granules may be considered as promising bioceramic alternative matrixes for bone regeneration and drug release application. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {Agglomeration, alkaline phosphatase, Apatite, article, Bioceramics, Biocompatible Materials, Biological Markers, Biological materials, Biological response, Biomaterials, Bone, bone morphogenetic protein 2, Bone morphogenetic protein-2, Bone Regeneration, cell adhesion, cell function, cell granule, cell migration, cell proliferation, Cells, Cultured, collagen type 1, Collagen type I, colony stimulating factor 1, Colony-stimulating factor, drug activity, Drug release, Durapatite, Expression levels, Fourier transform infrared spectroscopy, Fourier transforms, Granulation, Granules, growth rate, human, human cell, Human osteoblast-like cells, Humans, hydroxyapatite, In-vitro, infrared spectroscopy, Interconnected porosity, Macro-porosity, Macropores, Macroporosity, Macroporous, materials testing, matrix, Mercury (metal), Mercury intrusion porosimetry, Microscopy, Electron, Scanning, Microstructured, Nano-materials, Nano-structured, nanomaterial, Nanomaterials, Nanostructured materials, Nanostructures, osteoblast, Osteoblast response, Osteoblast-like cells, Osteoblasts, Osteoprotegerin, Phosphatases, polyurethan, Polyurethane sponge, Polyurethanes, porosity, Porous structures, Reverse Transcriptase Polymerase Chain Reaction, Scanning electron microscopy, SEM, Spectroscopy, Fourier Transform Infrared, Surface area, surface property, X ray diffraction, X-Ray Diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78249252998\&partnerID=40\&md5=09d51d6c49d9f10e6c2d479b1fe4ace0},
	author = {Laranjeira, M.S. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {2259,
	title = {An intrauterine pressure generator for educational simulation of labour and delivery},
	journal = {Medical Engineering and PhysicsMed. Eng. Phys.},
	volume = {32},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: MEPHEdoi: 10.1016/j.medengphy.2010.04.015PubMed ID: 20494606Language of Original Document: EnglishCorrespondence Address: Bastos, L.F.; Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Rua Dr. Roberto Frias, s/n, I302, 4200-465 Porto, Portugal; email: lbastos@fe.up.ptReferences: Carlin, A., Alfirevic, Z., Intrapartum foetal emergencies (2006) Semin Foetal Neonatal Med, 11, pp. 150-157;Draycott, T., Sibanda, T., Owen, L., Akande, V., Winter, C., Reading, S., Does training in obstetric emergencies improve neonatal outcome? (2006) BJOG, 113, pp. 177-182; 
Daniels, K., Lipman, S., Harney, K., Arafeh, J., Druzin, M., Use of simulation based team training for obstetric crises in resident education (2008) Simul Healthc, 3, pp. 154-160; 
Gardner, R., Walzer, T.B., Simon, R., Raemer, D.B., Obstetric simulation as a risk control strategy: course design and evaluation (2008) Simul Healthc, 3, pp. 119-127; 
Pliego, J.F., Wehbe-Janek, H., Rajab, M.H., Browning, J.L., Fothergill, R.E., Ob/Gyn boot camp using high-fidelity human simulators: enhancing residents{\textquoteright} perceived competency, confidence in taking a leadership role, and stress hardiness (2008) Simul Healthc, 3, pp. 82-89; 
Crofts, J.F., Bartlett, C., Ellis, D., Hunt, L.P., Fox, R., Draycott, T.J., Training for shoulder dystocia: a trial of simulation using low-fidelity and high-fidelity mannequins (2006) Obstet Gynecol (New York, 1953), 108, pp. 1477-1485; 
Gardner, R., Raemer, D.B., Simulation in obstetrics and gynecology (2008) Obstet Gynecol Clin North Am, 35, pp. 97-127; 
(2001) The use of electronic foetal monitoring: the use and interpretation of cardiotocography in intrapartum foetal surveillance, , Royal-College-of-Obstetricians-and-Gynaecologists, RCOG, London; 
(2006) Intrapartum foetal surveillance: clinical guidelines, , Royal-Australian-and-New-Zealand-College-of-Obstetricians-and-Gynaecologists, RANZCOG, Melbourne; 
Bracero, L.A., Roshanfekr, D., Byrne, D.W., Analysis of antepartum foetal heart rate tracing by physician and computer (2000) J Matern Foetal Med, 9, pp. 181-185; 
Doyle, C., Angelotti, T., Diagnosis of an unsuspected maternal hemorrhage via foetal heart rate tracing (2004) J Clin Anesth, 16, pp. 465-468; 
Donn, S.M., Sinha, S.K., Chiswick, M.L., (2002) Birth asphyxia and the brain: basic science and clinical implications, , Blackwell Publishing; 
Laros, J.R.K., Presidential address: medical-legal issues in obstetrics and gynecology (2005) Am J Obstet Gynecol, 192, pp. 1883-1889; 
Cusick, W., Smulian, J.C., Vintzileos, A.M., Intrapartum use of foetal heart rate monitoring, contraction monitoring, and amnioinfusion (1995) Clin Perinatol, 22, pp. 875-906; 
Vanner, T., Gardosi, J., Intrapartum assessment of uterine activity (1996) Baillieres Clin Obstet Gynaecol, 10, pp. 243-257; 
Bakker, P.C.A.M., Van Rijswijk, S., Van Rijsiwijk, S., van Geijn, H.P., Uterine activity monitoring during labor (2007) J Perinat Med, 35, pp. 468-477; 
Bakker, P.C.A.M., van Geijn, H.P., Uterine activity: implications for the condition of the foetus (2008) J Perinat Med, 36, pp. 30-37; 
Andersen, H.F., Barclay, M.L., A computer-model of uterine contractions based on discrete contractile elements (1995) Obstet Gynecol, 86, pp. 108-111. , J; 
Young, R.C., A computer model of uterine contractions based on action potential propagation and intercellular calcium waves (1997) Obstet Gynecol, 89, pp. 604-608. , A; 
Wray, S., Uterine contraction and physiological mechanisms of modulation (1993) Am J Physiol, 264, pp. C1-C18. , J; 
Wray, S., Kupittayanant, S., Shmygol, A., Smith, R.D., Burdyga, T., The physiological basis of uterine contractility: a short review (2001) Exp Physiol, 86, pp. 239-246; 
Vauge, C., Carbonne, B., Papiernik, E., Ferre, F., A mathematical model of uterine dynamics and its application to human parturition (2000) Acta Biotheor, 48, pp. 95-105. , J; 
Moreau, R., Pham, M.T., Redarce, T., Dupuis, O., Simulation of forceps extraction on a childbirth simulator (2008) Proceedings of the IEEE International Conference on Robotics and Automation (ICRA), pp. 1100-1105; 
Klavan, M., Laver, A.T., Boscola, M.A., (1977) Clinical concepts of foetal heart rate monitoring, , Hewlett-Packard Waltham, Mass; 
Parer, J.T., (1997) Handbook of foetal heart rate monitoring, , W.B. Saunders Company, Philadelphia; 
Maul, H., Maner, W.L., Olson, G., Saade, G.R., Garfield, R.E., Non-invasive transabdominal uterine electromyography correlates with the strength of intrauterine pressure and is predictive of labor and delivery (2004) J Matern Foetal Neonatal Med, 15, pp. 297-301; 
Cobo, E., Quintero, C.A., Strada, G., Zambrana, M.A., Uterine behavior in abruptio placentae. I. Contraction patterns and their reactivity to oxytocin (1965) Am J Obstet Gynecol, 93, pp. 1151-1156; 
Seitchik, J., Quantitating uterine contractility in clinical context (1981) Obstet Gynecol, 57, pp. 453-457; 
Burchell, H.J., Odendaal, H.J., de Wet, J.I., Badenhorst, P.N., Uterine contraction patterns in abruptio placentae (1985) S Afr Med J, 67, pp. 48-50; 
Harbert, G.M., Assessment of uterine contractility and activity (1992) Clin Obstet Gynecol, 35, pp. 546-558; 
Bruce, E.N., (2001) Biomedical signal processing and signal modeling, , Wiley-Interscience; 
Levinson, N., The Wiener RMS (root mean square) error criterion in filter design and prediction (1947) J Math Phys, 25, pp. 261-278; 
Paul, M.J., Smeltzer, J.S., Relationship of measured external tocodynamometry with measured internal uterine activity (1991) Am J Perinatol, 8, pp. 417-420; 
Miles, A.M., Monga, M., Richeson, K.S., Correlation of external and internal monitoring of uterine activity in a cohort of term patients (2001) Am J Perinatol, 18, pp. 137-140; 
van Meurs, W.L., Good, M.L., Lampotang, S., Functional anatomy of full-scale patient simulators (1997) J Clin Monit Comput, 13, pp. 317-324; 
van Meurs, W.L., Nikkelen, E., Good, M.L., Pharmacokinetic-pharmacodynamic model for educational simulations (1998) IEEE Trans Biomed Eng, 45, pp. 582-590
},
	month = {2010///},
	pages = {740 - 745},
	abstract = {Simulation provides a risk free and controllable environment for training of healthcare providers. The limited realism of available simulators and training programs impedes immersive training in obstetric emergencies. In developed countries, intrapartum monitoring in high-risk cases involves continuous evaluation of foetal heart rate and uterine contractions signals. We present an essential component of a high-fidelity simulator for normal and critical situations in labour and delivery, namely an intrauterine pressure generator. The signal model behind the generator consists of a truncated Gaussian curve with the programmable features: amplitude, frequency, duration, and resting tone. Through analysis of 44. h of physiological data, we demonstrate that the natural variability of these features and of the baseline pressure can be approximated by deterministic trends and stationary stochastic processes. Signal parameters can be controlled by simulation instructors, scripts, or other models to reflect different patients, pathologies, and evolving clinical situations. Twelve 40-min tracings reflecting three different patients in labour were presented to three clinical experts, who attributed similar realism scores to simulated and to real tracings. {\textcopyright} 2010 IPEM.},
	keywords = {article, Clinical situations, Computer simulation, delivery, Delivery, Obstetric, Developed countries, Educational simulations, Essential component, Female, Fetal monitoring, Gaussian curves, generator, Health care providers, Heart Rate, Fetal, Heart rates, High-fidelity simulators, Humans, Immersive, intrauterine pressure, labor, Labor, Obstetric, Labour and delivery, medical education, Medical educational simulation, muscle tone, Natural variability, normal distribution, Obstetrics, Physiological data, Pregnancy, Prenatal Diagnosis, Pressure, Pressure generators, priority journal, Random processes, Risk free, Signal generator, Signal generators, Signal models, Signal parameters, Signal Processing, Computer-Assisted, simulation, Stationary stochastic process, statistical model, statistical parameters, statistics, Stochastic Processes, Time Factors, Training program, Uterine activities, Uterine activity, Uterine Contraction, Uterine Monitoring, uterus contraction},
	isbn = {13504533 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955249563\&partnerID=40\&md5=42013058b547a71f970a3f7f1374caaf},
	author = {Bastos, L.F. and Lobo, M.F. and van Meurs, W.L. and Ayres-de-Campos, D.}
}
@article {2253,
	title = {The MEE principle in data classification: A perceptron-based analysis},
	journal = {Neural ComputationNeural Comp.},
	volume = {22},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1162/NECO_a_00013PubMed ID: 20569178Language of Original Document: EnglishCorrespondence Address: Silva, L.M.; Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Porto 4200-465, Portugal; email: lmsilva@fe.up.ptReferences: Alexandre, L.A., Marques de S{\'a}, J., Error entropy minimization for LSTM training (2006) Proceedings of the 16th International Conference on Artificial Neural Networks, pp. 244-253. , Berlin: Springer;Asuncion, A., Newman, D., (2007) UCI machine learning repository. Irvine University of California, Irvine, , http://www.ics.uci.edu/~mlearn/MLRepository.html, School of Information and Computer Sciences. Available online at; 
Bishop, C., (1995) Neural networks for pattern recognition, , New York: OxfordUniversity Press; 
Erdogmus, D., Hild K., II., Pr{\'\i}ncipe, J.C., Blind source separation using R{\'e}nyi{\textquoteright}s α-marginal entropies (2002) Neurocomputing, 49, pp. 25-38; 
Erdogmus, D., Pr{\'\i}ncipe, J.C., Comparison of entropy and mean square error criteria in adaptive system training using higher order statistics (2000) Proceedings of the Intl. Conf. on ICA and Signal Separation, pp. 75-80. , Berlin: Springer-Verlag; 
Erdogmus, D., Pr{\'\i}ncipe, J.C., An error-entropy minimization algorithm for supervised training of nonlinear adaptive systems (2002) IEEE Transactions on Signal Processing, 50 (7), pp. 1780-1786; 
Erdogmus, D., Pr{\'\i}ncipe, J.C., Convergence properties and data efficiency of the minimum error entropy criterion in adaline training (2003) IEEE Transactions on Signal Processing, 51 (7), pp. 1966-1978; 
Gokcay, E., Pr{\'\i}ncipe, J.C., Information theoretic clustering (2002) IEEE Trans. on Pattern Analysis and Machine Learning, 24 (2), pp. 158-171; 
Han, L., Kernel partial least squares (K-PLS) for scientific data mining. Unpublished doctoral dissertation (2007) Rensselaer Polytechnic Institute; 
Hild, I.I., Erdogmus, K., Pr{\'\i}ncipe D., J.C., Blind source separation using R{\'e}nyi{\textquoteright}s mutual information (2001) IEEE Signal Processing Letters, 8, pp. 174-176; 
Jacobs, R., Jordan, M., Nowlan, S., Hinton, G., Adaptive mixtures of local experts (1991) Neural Computation, 3, pp. 79-87; 
Jenssen, R., Hild, K., Erdogmus, D., Pr{\'\i}ncipe, J.-C., Eltoft, T., Clustering using R{\'e}nyis entropy (2003) Proceedings of the Int. Joint Conference on Neural Networks, pp. 523-528. , Piscataway, NJ: IEEE Press; 
Johnson, N., Kotz, S., (1970) Continuous univariate distributions, , Hoboken, NJ:Wiley; 
Kapur, J., Maximum-entropy models in science and engineering (1993), (rev. ed.). Hoboken, NJ: WileyLagarias, J., Reeds, J.A., Wright, M.H., Wright, P.E., Convergence properties of the nelder-mead simplex method in low dimensions (1998) SIAM Journal of Optimization, 9 (1), pp. 112-147; 
Linsker, R., Self-organization in a perceptual network (1988) IEEE Computer, 21, pp. 105-117; 
Liu, W., Pokharel, P., Pr{\'\i}ncipe, J., Correntropy: Properties and applications in non-gaussian signal processing (2007) IEEE Transactions on Signal Processing, 55 (11), pp. 5286-5298; 
Parzen, E., On the estimation of a probability density function and the mode (1962) Annals of Mathematical Statistics, 33, pp. 1065-1076; 
Pr{\'\i}ncipe, J.C., Xu, D., Fisher, J., Information theoretic learning (2000) Unsupervised adaptive filtering, 1, pp. 265-319. , S. Haykin (Ed.), Blind source separation, Hoboken, NJ: Wiley; 
R{\'e}nyi, A., (1970) Probability theory, , Dordrecht: North-Holland; 
Richard, M., Lippmann, R., Neural network classifiers estimate Bayesian a posteriori probabilities (1991) Neural Computation, 3, pp. 461-483; 
Santos, J., (2007) Data classification with neural networks and entropic criteria, , Unpublished doctoral dissertation, University of Porto; 
Santos, J.M., Marques de S{\'a}, J., Alexandre, L.A., Batch-sequential algorithm for neural networks trained with entropic criteria (2005) Proceedings of the International Conference on Artificial Neural Networks, pp. 91-96. , Berlin: Springer-Verlag; 
Santos, J.M., Marques de S{\'a}, J., Alexandre, L.A., Legclust-a clustering algorithm based on layered entropic subgraphs (2007) IEEE Transactions on Pattern Analysis and Machine Intelligence, 30 (1), pp. 62-75; 
Santos, J.M., Marques de S{\'a}, J., Alexandre, L.A., Sereno, F., Optimization of the error entropy minimization algorithm for neural network classification (2004) Intelligent engineering systems through artificial neural networks, pp. 81-86. , C. Dagli, A. Buczak, D. Enke, M. Embrechts, \& O. Ersoy (Eds.), Washington, DC: ASME; 
Silva, L.M., (2008) Neural networks with error-density risk functionals for data classification, , Unpublished doctoral dissertation, University of Porto; 
Silva, L.M., Embrechts, M., Santos, J.M., Marques de S{\'a}, J., The influence of the risk functional in data classification with MLPs (2008) Proceedings of the International Conference on Artificial Neural Networks, pp. 185-194. , Berlin: Springer-Verlag; 
Silva, L.M., Felgueiras, C., Alexandre, L.A., Marques de S{\'a}, J., Error entropy in classification problems: A univariate data analysis (2006) Neural Computation, 18 (9), pp. 2036-2061; 
Silva, L.M., Marques de S{\'a}, J., Alexandre, L.A., Neural network classification using Shannon{\textquoteright}s entropy (2005) Proceedings of the European Symposium on Artificial Neural Networks, pp. 217-222. , Bruges: d-side; 
Stoller, D., Univariate two-population distribution free discrimination (1954) Journal of the American Statistical Association, 49, pp. 770-777; 
Thompson, J., Tapia, R., (1978) Nonparametric probability density estimation, , Baltimore, MD: Johns Hopkins University Press; 
Watanabe, S., Pattern recognition as a quest for minimum entropy (1981) Pattern Recognition, 13 (5), pp. 381-387
},
	month = {2010///},
	pages = {2698 - 2728},
	abstract = {This letter focuses on the issue of whether risk functionals derived from information-theoretic principles, such as Shannon or R{\'e}nyi{\textquoteright}s entropies, are able to cope with the data classification problem in both the sense of attaining the risk functional minimum and implying the minimum probability of error allowed by the family of functions implemented by the classifier, here denoted by min Pe. The analysis of this so-called minimization of error entropy (MEE) principle is carried out in a single perceptron with continuous activation functions, yielding continuous error distributions. In spite of the fact that the analysis is restricted to single perceptrons, it reveals a large spectrum of behaviors that MEE can be expected to exhibit in both theory and practice. In what concerns the theoretical MEE, our study clarifies the role of the parameters controlling the perceptron activation function (of the squashing type) in often reaching the minimum probability of error. Our study also clarifies the role of the kernel density estimator of the error density in achieving the minimum probability of error in practice. {\textcopyright} 2010 Massachusetts Institute of Technology.},
	keywords = {algorithm, Algorithms, artificial intelligence, artificial neural network, automated pattern recognition, Computer simulation, entropy, letter, Mathematical Concepts, mathematical phenomena, Models, Statistical, Neural Networks (Computer), Pattern Recognition, Automated, standard, statistical model},
	isbn = {08997667 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78149334887\&partnerID=40\&md5=97c7445cfa4693d406d979afc4b42319},
	author = {Silva, L.M. and Marques de S{\'a}, J. and Alexandre, L.A.}
}
@article {2288,
	title = {A model for educational simulation of hemodynamic transitions at birth},
	journal = {Pediatric ResearchPediatr. Res.},
	volume = {67},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: PEREBdoi: 10.1203/PDR.0b013e3181c2def3Language of Original Document: EnglishCorrespondence Address: Van Meurs, W. L.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUP, Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: wvanmeurs@ineb.up.ptReferences: Van Meurs, W.L., Good, M.L., Lampotang, S., Functional anatomy of full-scale patient simulators (1997) J Clin Monit, 13, pp. 317-324;S{\'a} Couto, C.D., Van Meurs, W.L., Goodwin, J.A., Andriessen, P., A model for educational simulation of neonatal cardiovascular pathophysiology (2006) Simul Healthc, 1, pp. 4-9; 
Zijlmans, M., S{\'a}-Couto, C.D., Van Meurs, W.L., Goodwin, J.A., Andriessen, P., Corrected and improved model for educational simulation of neonatal cardiovascular pathophysiology (2009) Simul Healthc, 4, pp. 49-53; 
Goodwin, J.A., Van Meurs, W.L., S{\'a} Couto, C.D., Beneken, J.E., Graves, S.A., A model for educational simulation of infant cardiovascular physiology (2004) Anesth Analg, 99, pp. 1655-1664; 
M{\'e}nigault, E., Vieyres, P., Lepoivre, B., Durand, A., Pourcelot, L., Berson, M., Fetal heart modelling based on a pressure-volume relationship (1997) Med Biol Eng Comput, 35, pp. 715-721; 
Pennati, G., Bellotti, M., Fumero, R., Mathematical modeling of the human foetal cardiovascular system based on Doppler ultrasound data (1997) Med Eng Phys, 19, pp. 327-335; 
Huikeshoven, F., Coleman, T.G., Jongsma, H.W., Mathematical model of the fetal cardiovascular system: The uncontrolled case (1980) Am J Physiol, 239, pp. R317-R325; 
Mielke, G., Benda, N., Cardiac output and central distribution of blood flow in the human fetus (2001) Circulation, 103, pp. 1662-1668; 
Cunningham, G., Leveno, K., MacDonald, P., Gilstrap III, L., Gant, N., Hankins, G., Clark, S., The morphological and functional development of the fetus (1996) Williams Obstetrics, pp. 151-190. , Cunningham G, Leveno K, MacDonald P, Gilstrap III L, Gant N, Hankins G, Clark S (eds) 20th Ed. Appleton \& Lange, CT; 
Huikeshoven, F.J., Hope, I.D., Power, G.G., Gilbert, R.D., Longo, L.D., Mathematical model of fetal circulation and oxygen delivery (1985) Am J Physiol, 249, pp. R192-R202; 
Rasanen, J., Wood, D.C., Weiner, S., Ludomirski, A., Huhta, J.C., Role of the pulmonary circulation in the distribution of human fetal cardiac output during the second half of pregnancy (1996) Circulation, 94, pp. 1068-1073; 
Assali, N.S., Morris, J.A., Beck, R., Cardiovascular hemodynamics in the fetal lamb before and after lung expansion (1965) Am J Physiol, 208, pp. 122-129; 
Vilos, G.A., Liggins, G.C., Intrathoracic pressures in fetal sheep (1982) J Dev Physiol, 4, pp. 247-256; 
Hooper, S.B., Wallace, M.J., Role of the physicochemical environment in lung development (2006) Clin Exp Pharmacol Physiol, 33, pp. 273-279; 
Greenspan, J.S., Shaffer, T.H., Fox, W.W., Spitzer, A.R., Assisted ventilation: Physiologic implications and complications (1998) Fetal and Neonatal Physiology, 2. , Polin RA, Fox WW (eds) W.B. Saunders Company, Philadelphia, PA; 
Friedman, A.H., Fahey, J.T., The transitions from fetal to neonatal circulation: Normal responses and implications for infants with heart disease (1993) Semin Perinatol, 17, pp. 106-121; 
Ghanayem, N.S., Gordon, J.B., Modulation of pulmonary vasomotor tone in the fetus and neonate (2001) Respir Res, 2, pp. 139-144; 
Dawes, G.S., Mott, J.C., Widdicombe, J.G., Wyatt, D.G., Changes in the lungs of the new-born lamb (1953) J Physiol, 121, pp. 141-162; 
Brook, M.M., Heymann, M.A., Teitel, D.F., The heart (2001) Care of the High-Risk, , Klaus MH, Fanaroff AA (eds) 5th ed. W.B. Saunders Company, Philadelphia, PA; 
Gutyon, A.C., Fetal and neonatal physiology (1991) Textbook of Medical Physiology, , Guyton AC (ed) 8th ed. W.B. Saunders Company Philadelphia, PA; 
Lagercrantz, H., Bistoletti, P., Catecholamine release in the newborn infant at birth (1977) Pediatr Res, 11, pp. 889-893; 
Padbury, J.F., Diakomanolis, E.S., Hobel, C.J., Perelman, A., Fisher, D.A., Neonatal adaptation: Sympatho-adrenal response to umbilical cord cutting (1981) Pediatr Res, 15, pp. 1483-1487; 
Minoura, S., Gilbert, R.D., Postnatal change of cardiac function in lambs: Effects of ganglionic block and afterload (1987) J Dev Physiol, 9, pp. 123-135; 
Coskun, S., Y{\"u}ksel, H., Bilgi, Y., Lacin, S., Tansug, N., Onag, A., Non-invasive evaluation of the adaptations of cardiac function in the neonatal period: A comparison of healthy infants delivered by vaginal route and caesarean section (2001) Acta Med Okayama, 55, pp. 213-218; 
Kishkurno, S., Takahashi, Y., Harada, K., Ishida, A., Tamura, M., Takada, G., Postnatal changes in left ventricular volume and contractility in healthy term infants (1997) Pediatric Cardiology, 18 (2), pp. 91-95. , DOI 10.1007/s002469900123; 
Shiota, T., Harada, K., Takada, G., Left ventricular systolic and diastolic function during early neonatal period using transthoracic echocardiography (2002) Tohoku J Exp Med, 197, pp. 151-158; 
Walther, F.J., Benders, M.J., Leighton, J.O., Early changes in the neonatal circulatory transition (1993) J Pediatr, 123, pp. 625-632; 
Hiraishi, S., Misawa, H., Oguchi, K., Kadoi, N., Saito, K., Fujino, N., Hojo, M., Yashiro, K., Two-dimensional Doppler echocardiographic assessment of closure of the ductus arteriosus in normal newborn infants (1987) J Pediatr, 111, pp. 755-760; 
Migliavacca, F., Dubini, G., Pennati, G., Pietrabissa, R., Fumero, R., Hsia, T.Y., De Leval, M.R., Computational model of the fluid dynamics in systemic-to-pulmonary shunts (2000) J Biomech, 33, pp. 549-557; 
Teitel, D.F., Iwamoto, H.S., Rudolph, A.M., Effects of birth-related events on central blood flow patterns (1987) Pediatr Res, 22, pp. 557-566; 
Anderson, D.F., Bissonnette, J.M., Faber, J.J., Thornburg, K.L., Central shunt flows and pressures in the mature fetal lamb (1981) Am J Physiol, 241, pp. H60-H66; 
Agata, Y., Hiraishi, S., Oguchi, K., Misawa, H., Horiguchi, Y., Fujino, N., Yashiro, K., Shimada, N., Changes in left ventricular output from fetal to early neonatal life (1991) J Pediatr, 119, pp. 441-445; 
Harada, K., Shiota, T., Takahashi, Y., Tamura, M., Takada, G., Changes in the volume and performance of the left ventricle in the early neonatal period (1994) Early Hum Dev, 39, pp. 201-209; 
Moss, A.J., Emmanouilides, G.C., Adams, F.H., Chuang, K., Response of ductus arteriosus and pulmonary and systemic arterial pressure to changes in oxygen environment in newborn infants (1964) Pediatrics, 33, pp. 937-944; 
Emmanouilides, G.C., Moss, A.J., Monset-Couchard, M., Marcano, B.A., Rzeznic, B., Cardiac output in newborn infants (1970) Biol Neonate, 15, pp. 186-197; 
Emmanouilides, G.C., Moss, A.J., Duffie Jr., E.R., Adams, F.H., Pulmonary arterial pressure changes in human newborn infants from birth to 3 days of age (1964) J Pediatr, 65, pp. 327-333; 
Soni, N., Williams, P., Positive pressure ventilation: What is the real cost? (2008) Br J Anaesth, 101, pp. 446-457; 
Moss, A.J., Emmanouilides, G.C., Duffie Jr., E.R., Closure of the ductus arteriosus in the newborn infant (1963) Pediatrics, 32, pp. 25-30
},
	month = {2010///},
	pages = {158 - 165},
	abstract = {Birth is characterized by swift and complex transitions in hemodynamic and respiratory variables. Unrecognized pathologies or incidents may quickly become fatal or cause permanent damage. This article introduces an essential component of an acute perinatal care simulator, namely a model for educational simulation of normal hemodynamic transitions seen during and shortly after birth. We explicitly formulate educational objectives and adapt a preexisting model for the simulation of neonatal cardiovascular physiology to include essential aspects of fetal hemodynamics. From the scientific literature, we obtain model parameters that characterize these aspects quantitatively. The fetal model is controlled by a time-and event-based script of changes occurring at birth, such as onset of breathing and cord clamping, and the transitory phase up to 24 h after birth. Comparison of simulation results with published target data confirms that realistic simulated hemodynamic vital signs are achieved. Copyright {\textcopyright} 2010 International Pediatric Research Foundation, Inc.},
	keywords = {article, artificial ventilation, biological model, birth, Blood flow, blood flow velocity, blood pressure, breathing, breathing mechanics, cardiovascular function, clamp, comparative study, Computer simulation, Constriction, education, evaluation, fetus, hemodynamics, human, Humans, Infant, Newborn, ligation, Models, Cardiovascular, newborn, Parturition, perinatal care, priority journal, Regional Blood Flow, reproducibility, Reproducibility of Results, Respiration, Artificial, Respiratory Mechanics, scientific literature, simulator, stimulation, time, Time Factors, umbilical cord, vascularization, vital sign},
	isbn = {00313998 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77449090648\&partnerID=40\&md5=8b519ac5d166273410a6d24ec4d23578},
	author = {S{\'a}-Couto, C.D. and Andriessen, P. and van Meurs, W.L. and Ayres-de-Campos, D. and S{\'a}-Couto, P.M.}
}
@book {2263,
	title = {Optical flow based Arabidopsis thaliana root meristem cell division detection},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6112 LNCS},
	year = {2010},
	note = {Conference code: 81097Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-13775-4_22Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Campilho, A., Garcia, B., Toorn, H.V.D., Wijk, H.V., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48 (4), pp. 619-627. , DOI 10.1111/j.1365-313X.2006.02892.x;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res., 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A., Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo (2007) Motion{\textquoteright}07, p. 13; 
Beemster, G.T.S., Baskin, T.I., Analysis of Cell Division and Elongation Underlying the Developmental Acceleration of Root Growth in Arabidopsis thaliana (1998) Plant Physiology, 116 (4), pp. 1515-1526; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Rohr, K., Automated analysis of mitotic cell nuclei in 3rd fluorescence microscopy image sequences Workshop on Bio-Image Inf.: Bio. Imag., Comp. Vision and Data Mining (2008); 
Yang, F., Mackey, M.A., Ianzini, F., Gallardo, G., Sonka, M., Cell segmentation, tracking, and mitosis detection using temporal context (2005) Medical Image Computing and Computer-Assisted Intervention (MICCAI), pp. 302-309; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Comp. in Biology and Medicine, 39, pp. 785-793; 
Marcuzzo, M., Guichard, T., Quelhas, P., Maria Mendon{\c c}a, A., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Araujo, H., Mendon{\c c}a, A.M., Pinho, A.J., Torres, M.I. (eds.) IbPRIA 2009. Springer, Heidelberg; 
Pereira, C.S., Mendon{\c c}a, A.M., Campilho, A., Evaluation of contrast enhancement filters for lung nodule detection (2007) LNCS, 4633, pp. 878-888. , Kamel, M.S., Campilho, A. (eds.) ICIAR 2007. Springer, Heidelberg; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of arabidopsis thaliana root meristem in confocal microscopy (2004) LNCS, 3212, pp. 166-174. , Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. Springer, Heidelberg; 
Hartley, R., Zisserman, A., (2003) Multiple View Geometry in Computer Vision, , Cambridge University Press, Cambridge; 
Lowe, D.G., Distinctive image features from scale-invariant keypoints (2004) International Journal of Computer Vision, 60, pp. 91-110; 
Brox, T., Bruhn, A., Papenberg, N., Weickert, J., High accuracy optical flow estimation based on a theory for warping (2004) LNCS, 3024, pp. 25-36. , Pajdla, T., Matas, J(G.) (eds.) ECCV 2004. Springer, Heidelberg
},
	month = {2010///},
	pages = {217 - 226},
	address = {Povoa de Varzim},
	abstract = {The study of cell division and growth is a fundamental aspect of plant biology research. In this research the Arabidopsis thaliana plant is the most widely studied model plant and research is based on in vivo observation of plant cell development, by time-lapse confocal microscopy. The research herein described is based on a large amount of image data, which must be analyzed to determine meaningful transformation of the cells in the plants. Most approaches for cell division detection are based on the morphological analysis of the cells{\textquoteright} segmentation. However, cells are difficult to segment due to bad image quality in the in vivo images. We describe an approach to automatically search for cell division in the Arabidopsis thaliana root meristem using image registration and optical flow. This approach is based on the difference of speeds of the cell division and growth processes (cell division being a much faster process). With this approach, we can achieve a detection accuracy of 96.4\%. {\textcopyright} 2010 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Biology, Biology image processing, cell division detection, Cell divisions, cell proliferation, confocal microscopy, Detection accuracy, Growth process, Image data, Image quality, Image segmentation, Imaging systems, In-vivo, In-vivo images, Metadata, Morphological analysis, Optical data processing, Optical flows, Plant biology, Plant cells, research, Root meristem},
	isbn = {03029743 (ISSN); 3642137741 (ISBN); 9783642137747 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955346994\&partnerID=40\&md5=22ef87a91388dfe96acf9e4ac6ad4377},
	author = {Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2261,
	title = {Plasma surface modification of polycarbonate and poly(propylene) substrates for biomedical electrodes},
	journal = {Plasma Processes and PolymersPlasma Processes Polym.},
	volume = {7},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: Scopusdoi: 10.1002/ppap.200900176Language of Original Document: EnglishCorrespondence Address: Vaz, F.; Universidade do Minho, Centro de Fisica, Campus de Azur{\'e}m, 4800-058 Guimar{\~a}es, Portugal; email: fvaz@fisica.uminho.ptReferences: Moon, M.-W., Vaziri, A., (2009) Scripta Mater., 60, p. 44;Pandiyaraj, K., Selvarajan, V., Deshmukh, R.R., Gao, C., (2009) Appl. Surf. Sci., 255, p. 3965; 
Pandiyaraj, K., Selvarajan, V., Deshmukh, R.R., Gao, C., (2009) Vacuum, 83, p. 332; 
Tavares, C.J., Marques, S.M., Rebouta, L., Lanceros-M{\'e}ndez, S., Sencadas, V., Costa, C.M., Alves, E., Fernandes, A.J., (2008) Thin Solid Films, 517, p. 1161; 
Eom, J.S., Kim, S.H., (2008) Thin Solid Films, 516, p. 4530; 
Di Mundo, R., De Benedictis, V., Palumbo, F., D{\textquoteright}Agostino, R., (2009) Appl. Surf. Sci., 255, p. 5461; 
{\"O}zenbas, M., Tan, C., (1986) J. Cryst Growth, 78, p. 519; 
Goddard, J.M., Hotchkiss, J.H., (2007) Prog. Potym. Sci., 32, p. 698; 
Nathawat, R., Kumar, A., Kulshrestha, V., Vijay, Y.K., Kobayashi, T., Kanjilal, D., (2008) Nuel. Instrum. Methods Phys. Res. B, 266, p. 4749; 
Shin, Y.N., Kim, B.S., Ahn, H.H., Lee, J.H., Kim, K.S., Lee, J.Y., Kim, M.S., Lee, H.B., (2008) Appl. Surf. Sci., 255, p. 293; 
Lisboa, P., Gilliland, D., Ceccone, G., Valsesia, A., Rossi, F., (2006) Appl. Surf. Sci., 252, p. 4397; 
Muck, A., Svatos, A., (2007) Talanta, 74, p. 333; 
Sandoval-Paz, M.G., Ramirez-Bon, R., (2008) Thin Solid Films, 517, p. 6747; 
Park, S.K., Han, J.I., Kim, W.K., Kwak, M.G., (2001) Thin Solid Films, 397, p. 49; 
Pihan, S.A., Tsukruk, T., F{\"o}rch, R., (2009) Surf. Coat. Technol., 13, p. 1856; 
Dayss, E., Leps, G., Meinhardt, J., (1999) Surf. Coat Teehnol., 116, p. 986; 
Pandiyaraj, K.N., Selvarajan, V., Deshmukh, R.R., Bousmina, M., (2008) Surf. Coat Teehnol., 202, p. 4218; 
Cheng, C., Liye, Z., Zhan, R.-J., (2006) Surf. Coat Teehnol., 200, p. 6659; 
Lai, J., Sunderland, B., Xue, J., Yan, S., Zhao, W., Folkard, M., Michael, B.D., Wang, Y., (2006) Appl. Surf. Sci., 252, p. 3375; 
Phenton, M.J., Lovell-Hoare, M.C., Stevens, G.C., (2001) J. Phys. D: Appl. Phys., 34, p. 2754; 
Ozeki, K., Hirakuri, K.K., (2008) Appl. Surf. Sci., 254, p. 1614; 
Vallon, S., Dr{\'e}villon, B., Poncin-Epaillard, F., KlembergSapieha, J.E., Martinu, L., (1996) J. Vac. Sci. Teehnol. A, 14, p. 3194; 
Mirabedini, S.M., Arabi, H., Salem, A., Asiaban, S., (2007) Prog. Org. Coat., 60, p. 105; 
Kogelschatz, U., Elliason, B., Egli, W., (1999) Pure Appl. Chem., 71, p. 1819; 
Dorai, R., Kushner, M.J., (2003) J. Phys. D: Appl. Phys., 36, p. 666; 
Corval, G.J., Gray, D.G., Goring, D.A.I., (1976) J. Polym. Sci., Polym. Lett, 14, p. 231; 
Guimond, S., Radu, I., Czeremuszkin, G., Caisson, D.J., Wertheimer, M.R., (2002) Plasmas Polym., 7, p. 71; 
Guimond, S., Radu, I., Czeremuszkin, G., Wertheimer, M.R., (2002) Proc. 8th Int. Symp. High Pressure Low Temp. Plasma Chem., p. 400. , P{\"u}haj{\"a}rve, Estland; 
Kogelschatz, U., Elliason, B., Egli, W., (1999) Pure Appl. Chem., 71, p. 1819; 
Cho, J.-S., Kim, K.-H., Han, S., Beag, Y.-W., Koh, S.-K., (2003) Prog. Org. Coat., 48, p. 251; 
Baek, J.-Y., An, J.-H., Choi, J.-M., Park, K.-S., Lee, S.-H., (2008) Sens. Actuators A, 143, p. 423; 
Gruetzmann, A., Hansen, S., M{\"u}ller, J., (2007) Physiol Meas., 28, p. 1375; 
Ribeiro, E., Malczyk, A., Carvalho, S., Rebouta, L., Fernandes, I.V., Alves, E., Miranda, A.S., (2002) Surf. Coat. Teehnol, 151-152, p. 515; 
Sharma, R., Holcomb, E., Trigwell, S., Mazumder, M., (2007) J. Electrostat., 65, p. 269; 
Cunha, L.T., Pedrosa, P., J.Tavares, C., Alves, E., Vaz, F., Fonseca, C., (2009) Electrochim. Acta, 55, p. 59; 
M{\"u}ller, D.J.M., Janovjak, H., Lehto, T., Kuerschner, L., Anderson, K., (2002) Prog. Biophys. Mol. Biol., 79, p. 1; 
Wolansky, G., Marmur, A., (1999) Colloids Surf. A, 156, p. 381; 
Mohamed, S.H., Kappertz, O., Ngaruiya, J.M., Niemeier, T., Drese, R., Wakkad, M., Wuttig, M., (2004) Phys. Stat. Sol., 202, p. 90; 
Carrino, L., Moroni, G., Polini, W., (2002) J. Mater. Proc. Technol., 121, p. 373
},
	month = {2010///},
	pages = {676 - 686},
	abstract = {The effects of surface plasma treatments on the properties of PP and PC substrates were studied. Main results showed that the 0.48 Pa Ar treatment promoted the most favorable changes: roughness increased from 15 to 17 nm for PC and from 12 to 30 nm for PP, while the CA lowered from 95{\textdegree} to 59{\textdegree} and from 87{\textdegree} to 35{\textdegree} for PC and PP, respectively. The effects of the activation treatment on adhesion were checked by depositing a thin layer of TiN on both untreated and 0.48 Pa Ar plasma treated PC samples, and the adhesion to both substrates compared using the standard X-cut tape test. The Ar activated samples displayed better adhesion of the TiN film than the untreated ones, confirming the benefits of the surface changes induced by the plasma treatments. (Figure Presented) {\textcopyright} 2010 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {Activation treatment, Adhesion, Ar plasmas, biomedical electrodes, Deposition, Electrodes, Functional polymers, Plasma surface modifications, Plasma treatment, Plasma treatments, Plasmas, Polymers, Propylene, Surface changes, Surface modification, Surface plasma treatment, Thin layers, TiN films, Titanium nitride},
	isbn = {16128850 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956235411\&partnerID=40\&md5=e0146f15b9c6dbbc01005ef48ca837e0},
	author = {Pedrosa, P. and Chapp{\'e}, J.-M. and Fonseca, C. and Machado, A.V. and N{\'o}brega, J.M. and Vaz, F.}
}
@article {2252,
	title = {Proliferation and mineralization of bone marrow cells cultured on macroporous hydroxyapatite scaffolds functionalized with collagen type I for bone tissue regeneration},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32600Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Divis{\~a}o de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: 1 (3 dimethylaminopropyl) 3 ethylcarbodiimide, 1892-57-5, 25952-53-8, 7084-11-9; DNA, 9007-49-2; hydroxyapatite, 1306-06-5, 51198-94-8; methylene blue, 61-73-4; n hydroxysuccinimide, 6066-82-6; Collagen Type I; Cross-Linking Reagents; DNA, 9007-49-2; Durapatite, 1306-06-5; Methylene Blue, 61-73-4Manufacturers: SigmarecticelReferences: Nair, M.B., Suresh Babu, S., Varma, H.K., John, A., A triphasic ceramic-coated porous hydroxyapatite for tissue engineering application (2008) Acta Biomaterialia, 4 (1), pp. 173-181. , DOI 10.1016/j.actbio.2007.07.004, PII S174270610700102X;Navarro, M., Ginebra, M.P., Planell, J.A., Zeppetelli, S., Ambrosio, L., Development and cell response of a new biodegradable composite scaffold for guided bone regeneration (2004) Journal of Materials Science: Materials in Medicine, 15 (4), pp. 419-422. , DOI 10.1023/B:JMSM.0000021113.88702.9d; 
Nishikawa, M., Myoui, A., Ohgushi, H., Ikeuchi, M., Tamai, N., Yoshikawa, H., Bone tissue engineering using novel interconnected porous hydroxyapatite ceramics combined with marrow mesenchymal cells: Quantitative and three-dimensional image analysis (2004) Cell Transplant, 13, pp. 367-376; 
Fredericks, D.C., Bobst, J.A., Petersen, E.B., Nepola, J.V., Dennis, J.E., Caplan, A.I., Burgess, A.V., Schulz, O.H., Cellular interactions and bone healing responses to a novel porous tricalcium phosphate bone graft material (2004) Orthopedics, 27 (1 SUPPL.), pp. s167-s173; 
Wang, Y., Uemura, T., Dong, J., Kojima, H., Tanaka, J., Tateishi, T., Application of Perfusion Culture System Improves in Vitro and in Vivo Osteogenesis of Bone Marrow-Derived Osteoblastic Cells in Porous Ceramic Materials (2003) Tissue Engineering, 9 (6), pp. 1205-1214. , DOI 10.1089/10763270360728116; 
Wilson, C.E., De Bruijn, J.D., Van Blitterswijk, C.A., Verbout, A.J., Dhert, W.J., Design and fabrication of standardized hydroxyapatite scaffolds with a defined macro-architecture by rapid prototyping for bone-tissue-engineering research (2004) J Biomed Mater Res, 68, pp. 123-132; 
Rice, J.M., Hunt, J.A., Gallagher, J.A., Quantitative evaluation of the biocompatible and osteogenic properties of a range of biphasic calcium phosphate (BCP) granules using primary cultures of human osteoblasts and monocytes (2003) Calcif Tissue Int, 72, pp. 726-736; 
Zheng, Q., Zhu, T., Du, J., Wang, H., Li, S., Studies on the ossification of compound transplantation of porous TCP ceramics and bone marrow (1996) J Tongji Med Univ, 16, pp. 236-240; 
Goshima, J., Goldberg, V.M., Caplan, A.I., The osteogenic potential of culture-expanded rat marrow mesenchymal cells assayed in vivo in calcium phosphate ceramic blocks (1991) Clin Orthop Relat Res, 262, pp. 298-311; 
Cheung, H.S., Haak, M.H., Growth of osteoblasts on porous calcium phosphate ceramic: An in vitro model for biocompatibility study (1989) Biomaterials, 10, pp. 63-67; 
Barrere, F., Van Blitterswijk, C.A., De Groot, K., Bone regeneration: Molecular and cellular interactions with calcium phosphate ceramics (2006) Int J Nanomedicine, 1, pp. 317-332; 
Grynpas, M.D., Pilliar, R.M., Kandel, R.A., Renlund, R., Filiaggi, M., Dumitriu, M., Porous calcium polyphosphate scaffolds for bone substitute applications in vivo studies (2002) Biomaterials, 23, pp. 2063-2070; 
Heilmann, F., Standard, O.C., Muller, F.A., Hoffman, M., Development of graded hydroxyapatite/CaCO(3) composite structures for bone ingrowth (2007) J Mater Sci, 18, pp. 1817-1824; 
Hsu, Y.H., Turner, I.G., Miles, A.W., Mechanical characterization of dense calcium phosphate bioceramics with interconnected porosity (2007) J Mater Sci, 18, pp. 2319-2329; 
Huang, X., Miao, X., Novel porous hydroxyapatite prepared by combining H 2O 2 foaming with PU sponge and modified with PLGA and bioactive glass (2007) Journal of Biomaterials Applications, 21 (4), pp. 351-374. , DOI 10.1177/0885328206063905; 
Ito, Y., Tanaka, N., Fujimoto, Y., Yasunaga, Y., Ishida, O., Agung, M., Ochi, M., Bone formation using novel interconnected porous calcium hydroxyapatite ceramic hybridized with cultured marrow stromal stem cells derived from Green rat (2004) J Biomed Mater Res A, 69, pp. 454-461; 
Li, S.H., De Wijn, J.R., Layrolle, P., De Groot, K., Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering (2002) J Biomed Mater Res A, 61, pp. 109-120; 
Navarro, M., Del Valle, S., Martinez, S., Zeppetelli, S., Ambrosio, L., Planell, J.A., Ginebra, M.P., New macroporous calcium phosphate glass ceramic for guided bone regeneration (2004) Biomaterials, 25 (18), pp. 4233-4241. , DOI 10.1016/j.biomaterials.2003.11.012, PII S0142961203010780; 
Ramay, H.R.R., Zhang, M., Biphasic calcium phosphate nanocomposite porous scaffolds for load-bearing bone tissue engineering (2004) Biomaterials, 25 (21), pp. 5171-5180. , DOI 10.1016/j.biomaterials.2003.12.023, PII S0142961203011724; 
Wang, C., Kasuga, T., Nogami, M., Macroporous calcium phosphate glass-ceramic prepared by two-step pressing technique and using sucrose as a pore former (2005) JMater Sci, 16, pp. 739-744; 
Xin, R., Leng, Y., Chen, J., Zhang, Q., A comparative study of calcium phosphate formation on bioceramics in vitro and in vivo (2005) Biomaterials, 26 (33), pp. 6477-6486. , DOI 10.1016/j.biomaterials.2005.04.028, PII S0142961205003030; 
De Bruijn, J.D., Van Den Brink, I., Mendes, S., Dekker, R., Bovell, Y.P., Van Blitterswijk, C.A., Bone induction by implants coated with cultured osteogenic bone marrow cells (1999) Adv Dent Res, 13, pp. 74-81; 
De Groot, K., Effect of porosity and physicochemical properties on the stability, resorption, and strength of calcium phosphate ceramics (1988) Ann N Y Acad Sci, 523, pp. 227-233; 
De Groot, K., Wolke, J.G., Jansen, J.A., Calcium phosphate coatings for medical implants (1998) Proc Inst Mech Eng, 212, pp. 137-147; 
Van Blitterswijk, C.A., Grote, J.J., De Groot, K., Daems, W.T., Kuijpers, W., The biological performance of calcium phosphate ceramics in an infected implantation site. I. Biological performance of hydroxyapatite during Staphylococcus aureus infection (1986) J Biomed Mater Res, 20, pp. 989-1002; 
Arinzeh, T.L., Tran, T., Mcalary, J., Daculsi, G., A comparative study of biphasic calcium phosphate ceramics for human mesenchymal stem-cell-induced bone formation (2005) Biomaterials, 26 (17), pp. 3631-3638. , DOI 10.1016/j.biomaterials.2004.09.035, PII S0142961204008646; 
Kim, H.W., Knowles, J.C., Kim, H.E., Porous scaffolds of gelatin-hydroxyapatite nanocomposites obtained by biomimetic approach: Characterization and antibiotic drug release (2005) J Biomed Mater Res B Appl Biomater, 74, pp. 686-698; 
Legeros, R.Z., Properties of osteoconductive biomaterials: Calcium phosphates (2002) Clin Orthop Relat Res, 395, pp. 81-98; 
Mao, X.Z., Zhou, J.N., Hu, J.Z., Ruan, J.M., Wang, W.C., Ni, J.D., Hydroxyapatite/ tricalcium phosphate matrix scaffold as cell carriers in vitro (2004) Zhong Nan Da Xue Xue Bao Yi Xue Ban, 29, pp. 371-375; 
Rodriguez-Lorenzo, L.M., Vallet-Regi, M., Ferreira, J.M., Ginebra, M.P., Aparicio, C., Planell, J.A., Hydroxyapatite ceramic bodies with tailored mechanical properties for different applications (2002) J Biomed Mater Res A, 60, pp. 159-166; 
Tancred, D.C., Mccormack, B.A., Carr, A.J., A quantitative study of the sintering and mechanical properties of hydroxyapatite/ phosphate glass composites (1998) Biomaterials, 19, pp. 1735-1743; 
Rusnah, M., Andanastuti, M., Idris, B., The influence of sintering temperature on the porosity and strength of porous hydroxyapatite ceramics (2004) Med J Malaysia, 59 (SUPPL. B), pp. 158-159; 
Sanchez-Salcedo, S., Werner, J., Vallet-Regi, M., Hierarchical pore structure of calcium phosphate scaffolds by a combination of gel-casting and multiple tape-casting methods (2008) Acta Biomaterialia, 4, pp. 913-922; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci, 19, pp. 855-899; 
Gelse, K., P{\"o}schl, E., Aigner, T., Collagens - Structure, function, and biosynthesis (2003) Adv Rev, 55, pp. 1531-1546; 
Friess, W., Collagen - Biomaterial for drug delivery (1998) Eur J Pharm Biopharm, 45, pp. 113-136; 
Weadock, K.S., Miller, E.J., Bellincampi, L.D., Zawadsky, J.P., Dunn, M.G., Physical crosslinking of collagen fibers: Comparison of ultraviolet irradiation and dehydrothermal treatment (1995) J Biomed Mater Res, 29, pp. 1373-1379; 
Yoshioka, S.A., Goissis, G., Thermal and spectrophotometric studies of new crosslinking method for collagen matrix with glutaraldehyde acetals (2008) Journal of Materials Science: Materials in Medicine, 19 (3), pp. 1215-1223. , DOI 10.1007/s10856-007-0151-0; 
Wissink, M.J., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Binding and release of basic fibroblast growth factor from heparinized collagen matrices (2001) Biomaterials, 22, pp. 2291-2299; 
Wissink, M.J., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Immobilization of heparin to EDC/NHS-crosslinked collagen. Characterization and in vitro evaluation (2001) Biomaterials, 22, pp. 151-163; 
Wissink, M.J., Beernink, R., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices (2000) J Control Release, 64, pp. 103-114; 
Wissink, M.J., Beernink, R., Scharenborg, N.M., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Endothelial cell seeding of (heparinized) collagen matrices: Effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors (2000) J Control Release, 67, pp. 141-155; 
Wissink, M.J.B., Van Luyn, M.J.A., Beernink, R., Dijk, F., Poot, A.A., Engbers, G.H.M., Beugeling, T., Feijen, J., Endothelial cell seeding on crosslinked collagen: Effects of crosslinking on endothelial cell proliferation and functional parameters (2000) Thrombosis and Haemostasis, 84 (2), pp. 325-331; 
Teixeira, S., Oliveira{\l}, S., Ferraz{\l}, M.P., Monteiro, F.J., Three dimensional macroporous calcium phosphate scaffolds for bone tissue engineering (2008) Key Eng Mater, 361-363, pp. 947-950; 
Teixeira, S., Rodriguez, M., Pena, P., De Aza, A., De Aza, S., Ferraz, M., Monteiro, F., Physical characterization of hydroxyapatite porous scaffolds for tissue engineering (2009) Mater Sci Eng: C, 29, pp. 1510-1514
},
	month = {2010///},
	pages = {1 - 8},
	abstract = {This study concerns the preparation and in vitro characterization of functionalized hydroxyapatite (HA) porous scaffolds, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite scaffolds were prepared using the polymer replication method, and, after being submitted to a specific sintering cycle, collagen Type I was incorporated on the surface. After the coating procedure, collagen was crosslinked using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) conjugation method. In this study, hydroxyapatite scaffolds with uncrosslinked and cross-linked Type I collagen were evaluated. Cell morphology and deposition of extracellular matrix were assessed by scanning electron microscopy, whereas cell distribution was visualized by means of methylene blue staining. MTS and total DNA quantification assays were used to evaluate the viability and proliferation of human bone marrow cells cultured on all the materials for 28 days. Results showed that the cells were able to adhere, proliferate, and form a mineralized matrix on the surface of all the materials. Furthermore, the cells were able to spread from one pore to another and form cell clusters. The results show that these scaffolds are good candidates to serve as drug delivery vehicles and for tissue engineering purposes. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {1 (3 dimethylaminopropyl) 3 ethylcarbodiimide, adult, Animal cell culture, Apatite, article, Automobile exhibitions, Bone, bone marrow cell, Bone Marrow Cells, bone matrix, bone mineralization, Bone Regeneration, bone tissue, Bone tissue regeneration, Calcification, Physiologic, cell adhesion, Cell clusters, Cell culture, Cell distribution, Cell morphology, cell proliferation, cell structure, cell viability, Cells, Cultured, cellular distribution, Ceramic materials, chemistry, Coating procedures, Collagen, collagen type 1, Collagen type I, conjugation, controlled study, cross linking, cross linking reagent, Cross-Linking Reagents, Crosslinked, cytology, Cytotoxicity, DNA, DNA quantification, drug conjugation, Drug delivery, drug delivery system, Drug delivery vehicles, drug effect, Drug-delivery systems, Durapatite, Extracellular matrices, extracellular matrix, Functionalized, human, Human bone marrow cells, human cell, Humans, hydroxyapatite, in vitro study, In-vitro, Macroporous, Macroporous ceramics, male, matrix, metabolism, methylene blue, Methylene blue staining, Microscopy, Electron, Scanning, molecular scaffold, n hydroxysuccinimide, N-hydroxysuccinimide, polymer, Polymer replication, porosity, Porous scaffold, Scaffolds, Scanning electron microscopy, Sintering, Sintering cycles, spectrometry, Spectrometry, X-Ray Emission, Tissue engineering, tissue scaffold, Tissue Scaffolds, Type I collagen, Young Adult},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956483928\&partnerID=40\&md5=de85a8bfe5af88cbc45e80302ab4dfd4},
	author = {Teixeira, S. and Fernandes, M.H. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2255,
	title = {The stability of self-assembled monolayers with time and under biological conditions},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {94},
	year = {2010},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32746PubMed ID: 20336761Language of Original Document: EnglishCorrespondence Address: Barbosa, M. A.; Divis{\~a}o de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: mbarbosa@ineb.up.ptChemicals/CAS: sulfur, 13981-57-2, 7704-34-9; Culture Media; Ethanol, 64-17-5; Sulfhydryl Compounds; Sulfur, 7704-34-9; Water, 7732-18-5References: Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man- Made surfaces with proteins and cells (1996) Annual Review of Biophysics and Biomolecular Structure, 25, pp. 55-78;Mrksich, M., Dike, L.E., Tien, J., Ingber, D.E., Whitesides, G.M., Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver (1997) Exp Cell Res, 235, pp. 305-313; 
Flynn, N.T., Tran, T.N.T., Cima, M.J., Langer, R., Long-term stability of self-assembled monolayers in biological media (2003) Langmuir, 19, pp. 10909-10915; 
Willey, T.M., Vance, A.L., Van Buuren, T., Bostedt, C., Terminello, L.J., Fadley, C.S., Rapid degradation of alkanethiol-based self-assembled monolayers on gold in ambient laboratory conditions (2005) Surf Sci, 576, pp. 188-196; 
Jones, J.A., Qin, L.A., Meyerson, H., Kwon, I.K., Matsuda, T., Anderson, J.M., Instability of self-assembled monolayers as a model material system for macrophage/FBGC cellular behavior (2008) J Biomed Mater Res Part A, 86 A, pp. 261-268; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- And methyl-terminated alkanethiols self-assembled monolayers (2003) Journal of Biomedical Materials Research - Part a, 67 (1), pp. 158-171; 
Martins, M.C.L., Fonseca, C., Barbosa, M.A., Ratner, B.D., Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry (2003) Biomaterials, 24, pp. 3697-3706; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Sastry, M., A note on the use of ellipsometry for studying the kinetics of formation of self-assembled monolayers (2000) Bull Mater Sci, 23, pp. 159-163; 
Castner, D.G., Hinds, K., Grainger, D.W., X-ray photoelectron spectroscopy sulfur 2p study of organic thiol and disulfide binding interactions with gold surfaces (1996) Langmuir, 12, pp. 5083-5086; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy, and electrochemistry (1987) J Am Chem Soc, 109, pp. 3559-3568; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res A, 89, pp. 642-653; 
Martins, M.C.L., Curtin, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res Part A, 88 A, pp. 162-173; 
Chidsey, C.E.D., Loiacono, D.N., Chemical functionality in self-assembled monolayers: Structural and electrochemical properties (1990) Langmuir, 6, pp. 682-691; 
Martins, M.C., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on cibacron blue F3G-A immobilized onto oligo(ethylene glycol)- Terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14, pp. 945-954; 
Ahn, H.S., Cuong, P.D., Park, S., Kim, Y.W., Lim, J.C., Effect of molecular structure of self-assembled monolayers on their tribological behaviors in nano- and microscales (2003) Wear, 255, pp. 819-825; 
Barrias, C.C., Martins, C.L., Miranda, C.S., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: Effect of surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
Martins, M.C., Fonseca, C., Barbosa, M.A., Ratner, B.D., Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry (2003) Biomaterials, 24, pp. 3697-3706; 
Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-terminated self-assembled monolayers (2005) Biomaterials, 26, pp. 3891-3899; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Adhesion of human leukocytes to biomaterials: An in vitro study using alkanethiolate monolayers with different chemically functionalized surfaces (2003) J Biomed Mater Res A, 65, pp. 429-434; 
Cavalleri, O., Gonella, G., Terreni, S., Vignolo, M., Pelori, P., Floreano, L., Morgante, A., Rolandi, R., High resolution XPS of the S 2p core level region of the L-cysteine/gold interface (2004) J Phys: Condens Matter, 16, pp. S2477; 
Petrovykh, D.Y., Kimura-Suda, H., Opdahl, A., Richter, L.J., Tarlov, M.J., Whitman, L.J., Alkanethiols on platinum: Multicomponent selfassembled monolayers (2006) Langmuir, 22, pp. 2578-2587; 
Rieley, H., Price, N.J., Smith, T.L., Yang, S.H., Photo-oxidation and photo-reduction in alkylthiol monolayers self-assembled on gold (1996) J Chem Soc Faraday Trans, 92, pp. 3629-3634; 
Raiber, K., Terfort, A., Benndorf, C., Krings, N., Strehblow, H.-H., Removal of self-assembled monolayers of alkanethiolates on gold by plasma cleaning (2005) Surf Sci, 595, pp. 56-63; 
Molina, R., Espinos, J.P., Yubero, F., Erra, P., Gonzalez-Elipe, A.R., XPS analysis of down stream plasma treated wool: Influence of the nature of the gas on the surface modification of wool (2005) Appl Surf Sci, 252, pp. 1417-1429; 
Love, J.C., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1170; 
Allara, D.L., Critical issues in applications of self-assembled monolayers (1995) Biosens Bioelectron, 10, pp. 771-783; 
Horn, A.B., Russell, D.A., Shorthouse, L.J., Simpson, T.R.E., Ageing of alkanethiol self-assembled monolayers (1996) J Chem Soc Faraday Trans, 92, pp. 4759-4762; 
Li, Y., Huang, J., McIver, R.T., Hemminger, J.C., Characterization of thiol self-assembled films by laser desorption Fourier transform mass spectrometry (1992) J Am Chem Soc, 114, pp. 2428-2432; 
Schoenfisch, M.H., Pemberton, J.E., Air stability of alkanethiol self-assembled monolayers on silver and gold surfaces (1998) J Am Chem Soc, 120, pp. 4502-4513; 
Mani, G., Johnson, D.M., Marton, D., Dougherty, V.L., Feldman, M.D., Patel, D., Ayon, A.A., Agrawal, C.M., Stability of self-assembled monolayers on titanium and gold (2008) Langmuir, 24, pp. 6774-6784; 
Tarlov, M.J., Newman, J.G., Static secondary ion mass spectrometry of self-assembled alkanethiol monolayers on gold (1992) Langmuir, 8, pp. 1398-1405; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Huang, J., Dahlgren, D.A., Hemminger, J.C., Photopatterning of self-assembled alkanethiolate monolayers on gold: A simple monolayer photoresist utilizing aqueous chemistry (1994) Langmuir, 10, pp. 626-628
},
	month = {2010///},
	pages = {833 - 843},
	abstract = {The stability of self-assembled monolayers (SAMs) of different functionalities (CH                        3
},
	keywords = {Animals, article, Binding energy, Biological conditions, Biological studies, Body fluids, Cattle, Cell culture, Culture Media, Ethanol, Female, Fetal bovine serum, In-cell, materials testing, measurement, molecular stability, Nitrogen chamber, nonhuman, Organic polymers, Oxidation, Oxidation-Reduction, Oxidized sulfur, Photodegradation, Photoelectron spectroscopy, Sams, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, Stability, Storage condition, Sulfhydryl Compounds, Sulfur, Surface Properties, Water, wettability, X ray photoelectron spectroscopy, XPS, XPS spectra},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77954806701\&partnerID=40\&md5=9c561e8b43c045cdeb8d80a42148c6ca},
	author = {Maciel, J. and Martins, M.C.L. and Barbosa, M.A.}
}
@article {2256,
	title = {Synthesis and characterization of HAp nanorods from a cationic surfactant template method},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {21},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-010-4122-5PubMed ID: 20623177Language of Original Document: EnglishCorrespondence Address: Moreira, J. A.; IFIMUP, IN-Institute of Nanoscience and Nanotechnology, Universidade Do Porto, Rua do Campo Alegre 687, Porto 4169-007, Portugal; email: jamoreir@fc.up.ptChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; hydroxyapatite, 1306-06-5, 51198-94-8; Cations; Cetrimonium Compounds; Surface-Active Agents; cetrimonium, 6899-10-1References: Rao, C.N.R., Cheetham, A.K., Materials science at the nanoscale (2006) Nanomaterials Handbook, pp. 1-2. , Y. Gogotsi (eds). CRC Press, Taylor \& Francis Group New York;Guo, Y., Shi, D., Lian, J., Zdw, W., Cho, H., Liu, G., Quantum dot conjugated hydroxylapatite nanoparticles for in vivo imaging (2008) Nanotechnology, 19, 6p. , 175102; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Production of porous hydroxyapatite with potential for controlled drug delivery (2004) Mater Sci Forum, 455-456, pp. 358-360. , 10.4028/www.scientific.net/MSF.455-456.358; 
Vallet-Regi, M., Ceramics for medical applications (2001) Journal of the Chemical Society, Dalton Transactions, (2), pp. 97-108. , DOI 10.1039/b007852m; 
Dorozhkin, S.V., Epple, M., Biological and medical significance of calcium phosphates (2002) Angewandte Chemie - International Edition, 41 (17), pp. 3130-3146. , DOI 10.1002/1521-3773(20020902)41:17<3130::AID-ANIE3130>3.0.CO;2-1; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.M., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80. , 1:CAS:528:DC\%2BD2cXnvFWiurk\%3D; 
Cengiz, B., Gokce, Y., Yildiz, N., Aktas, Z., Calimi, A., Synthesis and characterization of hydroxyapatite nanoparticles (2008) Colloids Surf A Physichochem Eng Aspects, 322, pp. 29-33. , 1:CAS:528:DC\%2BD1cXmt1OqtbY\%3D 10.1016/j.colsurfa.2008.02.011; 
Ying, J.Y., Mehnert, P.C., Wong, M.S., Synthesis and application of supramolecular-templated mesoporous materials (1999) Angew Chem Int Ed, 38, pp. 56-77. , 1:CAS:528:DyaK1MXpsValtg\%3D\%3D 10.1002/(SICI)1521-3773(19990115)38:1/ 2<56::AID-ANIE56>3.0.CO;2-E; 
Cates, M.E., Fielding, S.M., Rheology of giant micelles (2006) Advances in Physics, 55 (7-8), pp. 799-879. , DOI 10.1080/00018730601082029, PII T6J4U87W3G5RTN2J; 
Nagarajan, R., Molecular thermodynamics of giant micelles (2007) Giant Micelles-properties and Applications, pp. 2-5. , R. Zana E.W. Kaler (eds). CRC Pess, Taylor \& Francis Group New York; 
Li, Y., Tjandra, W., Tam, K.C., Synthesis of nanoporous hydroxyapatite using cationic surfactants as templates (2008) Mater Res Bull, 43, pp. 2318-2326. , 1:CAS:528:DC\%2BD1cXnsVSrtr4\%3D 10.1016/j.materresbull.2007.08.008; 
Yao, J., Tjandra, W., Chen, Y.Z., Tam, K.C., Mab, J., Soh, B., Hydroxyapatite nanostructure material derived using cationic surfactant as a template (2003) J Mater Chem, 13, pp. 3053-3057. , 1:CAS:528:DC\%2BD3sXptlemsro\%3D 10.1039/b308801d; 
Berret, J.F., Rheology of wormlike micelles: Equilibrium properties and shear banding transition (2006) Molecular Gels. Materials with Self-assembled Fibrillar Networks, pp. 667-720. , R.G. Weiss P. Terech (eds). Springer Dordrecht; 
Kresge, C.T., Leonowicz, M.E., Roth, W.J., Vartuli, J.C., Beck, J.S., Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism (1992) Nature, 359 (6397), pp. 710-712. , DOI 10.1038/359710a0; 
Beck, J.S., Vartuli, J.C., Roth, W.J., Leonowicz, M.E., Kresge, C.T., Schmitt, K.D., A new family of mesoporous molecular sieves prepared with liquid crystal templates (1992) J Am Chem Soc, 114, pp. 10834-10843. , 1:CAS:528:DyaK38Xms1entr8\%3D 10.1021/ja00053a020; 
Li, Y.Y., Li, J., Nakajima, B., Nanostructured porous biomaterials for controlled drug release systems (2008) Biomaterials-fabrication and Processing, pp. 196-206. , K.C. Paul L. Xuanyong (eds). CRC Press, Taylor \& Francis Group New York. 10.1201/9780849379741; 
Herzberg, G., (1945) The Infrared and Raman Spectra of Poliatomic Molecules, , D van Nostrand Company, Inc. New York; 
Awonusi, A., Morris, M.D., Tecklenburg, M.M.J., Carbonate assignment and calibration in the Raman spectrum of apatite (2007) Calcified Tissue International, 81 (1), pp. 46-52. , DOI 10.1007/s00223-007-9034-0; 
Fowler, B.O., Infrared studies of apatites. I. Vibrational assignments for calcium, strontium, and barium hydroxyapatites utilizing isotopic substitution (1974) Inorg Chem, 13, pp. 194-207. , 1:CAS:528:DyaE2cXlsVWktw\%3D\%3D 10.1021/ic50131a039; 
Aza, P.N., Santos, C., Pazo, A., Aza, S., Cusc{\'o}, R., Artus, L., Vibrational properties of calcium phospate coumpounds. 1. Raman spectrum of β-tricalcium phosphate (1997) Chem Mater, 9, pp. 912-915. , 10.1021/cm960425d
},
	month = {2010///},
	pages = {2543 - 2549},
	abstract = {Hydroxyapatite (HAp) [Ca                        10(PO
                        4)
                        6(OH) 
                        2] nanorods were synthesized using a surfactant templating method, with cetyltrimethylammonium bromide (CTAB) micelles acting as template for HAp growth. The effects of the sintering temperature on the morphological and crystallographic characteristics and on chemical composition of the "as-prepared" structures are discussed. The experimental results show that low heat-treatment temperatures are preferred in order to obtain high quality nanorods, with diameters ranging between 20 and 50 nm. High heat-treatment temperatures enhance the thermal decomposition of HAp into other calcium phosphate compounds, and the sintering of particles into micrometer ball-like structures. The stability of aqueous suspensions of HAp nanorods is also discussed. {\textcopyright} 2010 Springer Science+Business Media, LLC.
},
	keywords = {Ammonium compounds, Aqueous suspensions, article, Bromine compounds, Calcium, calcium phosphate, cationic surfactant, Cationic surfactants, Cations, Cetrimonium Compounds, Cetyltrimethylammonium bromide, chemical composition, Chemical compositions, Chemical structure, Crystallographic characteristics, Dyes, Heat treatment temperature, High quality, hydroxyapatite, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, nanorod, Nanorods, Nanotubes, Phosphate compounds, priority journal, Pyrolysis, Scanning electron microscopy, Sintering, Sintering temperatures, Surface-Active Agents, Surfactant template method, Surfactant templating, Suspensions (fluids), synthesis, Synthesis and characterizations, Thermal decompositions, X-Ray Diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956933849\&partnerID=40\&md5=e2701a45f87b57009484629157a4bc95},
	author = {Coelho, J.M. and Moreira, J.A. and Almeida, A. and Monteiro, F.J.}
}
@article {2275,
	title = {Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {143},
	year = {2010},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2010.01.018PubMed ID: 20093157Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; macrogol, 25322-68-3; polyethyleneimine, 74913-72-7; DNA, 9007-49-2; Imines; Peptide Fragments; Polyethylenes; Tetanus Toxin; poly(ethylene imine); tetanus toxin fragment CReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov. Today, 1 (2), pp. 177-185;Sahenk, Z., Neurotrophins and peripheral neuropathies (2006) Brain Pathol., 16 (4), pp. 311-319; 
Apfel, S.C., Kessler, J.A., Neurotrophic factors in the therapy of peripheral neuropathy (1995) Baillieres Clin. Neurol., 4 (3), pp. 593-606; 
Apfel, S.C., Is the therapeutic application of neurotrophic factors dead? (2002) Ann. Neurol., 51 (1), pp. 8-11; 
Glorioso, J.C., Mata, M., Fink, D.J., Therapeutic gene transfer to the nervous system using viral vectors (2003) J. Neurovirol., 9 (2), pp. 165-172; 
Boussif, O., Lezoualc{\textquoteright}h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B., A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine (1995) Proc. Natl. Acad. Sci. U.S.A., 92 (16), pp. 7297-7301; 
Coll, J.L., Chollet, P., Brambilla, E., Desplanques, D., Behr, J.P., Favrot, M., In vivo delivery to tumors of DNA complexed with linear polyethylenimine (1999) Hum. Gene Ther., 10 (10), pp. 1659-1666; 
Schaffer, D.V., Lauffenburger, D.A., Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery (1998) J. Biol. Chem., 273 (43), pp. 28004-28009; 
Zanta, M.A., Boussif, O., Adib, A., Behr, J.P., In vitro gene delivery to hepatocytes with galactosylated polyethylenimine (1997) Bioconjug. Chem., 8 (6), pp. 839-844; 
Diebold, S.S., Lehrmann, H., Kursa, M., Wagner, E., Cotten, M., Zenke, M., Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection (1999) Hum. Gene Ther., 10 (5), pp. 775-786; 
Blessing, T., Kursa, M., Holzhauser, R., Kircheis, R., Wagner, E., Different strategies for formation of PEGylated EGF-conjugated PEI/DNA complexes for targeted gene delivery (2001) Bioconjug. Chem., 12 (4), pp. 529-537; 
Erbacher, P., Remy, J.S., Behr, J.P., Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway (1999) Gene Ther., 6 (1), pp. 138-145; 
Kircheis, R., Kichler, A., Wallner, G., Kursa, M., Ogris, M., Felzmann, T., Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery (1997) Gene Ther., 4 (5), pp. 409-418; 
Park, I.K., Lasiene, J., Chou, S.H., Horner, P.J., Pun, S.H., Neuron-specific delivery of nucleic acids mediated by Tet1-modified poly(ethylenimine) (2007) J. Gene Med., 9 (8), pp. 691-702; 
Kwon, E.J., Bergen, J.M., Park, I.K., Pun, S.H., Peptide-modified vectors for nucleic acid delivery to neurons (2008) J. Control. Release, 132 (3), pp. 230-235; 
Price, D.L., Griffin, J., Young, A., Peck, K., Stocks, A., Tetanus toxin: direct evidence for retrograde intraaxonal transport (1975) Science, 188 (4191), pp. 945-947; 
Figueiredo, D.M., Hallewell, R.A., Chen, L.L., Fairweather, N.F., Dougan, G., Savitt, J.M., Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport (1997) Exp. Neurol., 145 (2 PART 1), pp. 546-554; 
Fishman, P.S., Savitt, J.M., Farrand, D.A., Enhanced CNS uptake of systemically administered proteins through conjugation with tetanus C-fragment (1990) J. Neurol. Sci., 98 (2-3), pp. 311-325; 
Coen, L., Osta, R., Maury, M., Brulet, P., Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system (1997) Proc. Natl. Acad. Sci. U.S.A., 94 (17), pp. 9400-9405; 
Knight, A., Carvajal, J., Schneider, H., Coutelle, C., Chamberlain, S., Fairweather, N., Non-viral neuronal gene delivery mediated by the HC fragment of tetanus toxin (1999) Eur. J. Biochem., 259 (3), pp. 762-769; 
Carlisle, R.C., Etrych, T., Briggs, S.S., Preece, J.A., Ulbrich, K., Seymour, L.W., Polymer-coated polyethylenimine/DNA complexes designed for triggered activation by intracellular reduction (2004) J. Gene Med., 6 (3), pp. 337-344; 
Goula, D., Remy, J.S., Erbacher, P., Wasowicz, M., Levi, G., Abdallah, B., Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system (1998) Gene Ther., 5 (5), pp. 712-717; 
Sinha, K., Box, M., Lalli, G., Schiavo, G., Schneider, H., Groves, M., Analysis of mutants of tetanus toxin Hc fragment: ganglioside binding, cell binding and retrograde axonal transport properties (2000) Mol. Microbiol., 37 (5), pp. 1041-1051; 
Ellman, G.L., Tissue sulfhydryl groups (1959) Arch. Biochem. Biophys., 82 (1), pp. 70-77; 
(1993) Iodine-125, a Guide to Radioionation Techniques, , Amersham Life Science; 
O{\textquoteright}brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) Eur. J. Biochem., 267 (17), pp. 5421-5426; 
Pollard, H., Remy, J.S., Loussouarn, G., Demolombe, S., Behr, J.P., Escande, D., Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells (1998) J. Biol. Chem., 273 (13), pp. 7507-7511; 
Neu, M., Fischer, D., Kissel, T., Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives (2005) J. Gene Med., 7 (8), pp. 992-1009; 
Moffatt, S., Papasakelariou, C., Wiehle, S., Cristiano, R., Successful in vivo tumor targeting of prostate-specific membrane antigen with a highly efficient J591/PEI/DNA molecular conjugate (2006) Gene Ther., 13 (9), pp. 761-772; 
Kircheis, R., Ostermann, E., Wolschek, M.F., Lichtenberger, C., Magin-Lachmann, C., Wightman, L., Tumor-targeted gene delivery of tumor necrosis factor-alpha induces tumor necrosis and tumor regression without systemic toxicity (2002) Cancer Gene Ther., 9 (8), pp. 673-680; 
Bellomo, G., Vairetti, M., Stivala, L., Mirabelli, F., Richelmi, P., Orrenius, S., Demonstration of nuclear compartmentalization of glutathione in hepatocytes (1992) Proc. Natl. Acad. Sci. U.S.A., 89 (10), pp. 4412-4416; 
Liu, H.S., Jan, M.S., Chou, C.K., Chen, P.H., Ke, N.J., Is green fluorescent protein toxic to the living cells? (1999) Biochem. Biophys. Res. Commun., 260 (3), pp. 712-717; 
Baens, M., Noels, H., Broeckx, V., Hagens, S., Fevery, S., Billiau, A.D., The dark side of EGFP: defective polyubiquitination (2006) PLoS One, 1, pp. e54; 
Taghizadeh, R.R., Sherley, J.L., CFP and YFP, but not GFP, provide stable fluorescent marking of rat hepatic adult stem cells (2008) J. Biomed. Biotechnol., 2008. , 453590; 
Liu, J.K., Tenga, Q.S., Garrity-Moses, M., Federici, T., Tanase, D., Imperiale, M.J., A novel peptide defined through phage display for therapeutic protein and vector neuronal targeting (2005) Neurobiol. Dis., 19 (3), pp. 407-418; 
Fishman, P.S., Carrigan, D.R., Retrograde transneuronal transfer of the C-fragment of tetanus toxin (1987) Brain Res., 406 (1-2), pp. 275-279
},
	month = {2010///},
	pages = {350 - 358},
	abstract = {A simple, safe and efficient system that can specifically transfect peripheral sensorial neurons can bring new answers to address peripheral neuropathies. A multi-component non-viral gene delivery vector targeted to peripheral nervous system cells was developed, using poly(ethylene imine) (PEI) as starting material. A binary DNA/polymer complex based on thiolated PEI (PEISH) was optimized, considering complex size and zeta potential and the ability to transfect a sensorial neuron cell line (ND7/23). The 50. kDa non-toxic fragment from tetanus toxin (HC), which has been previously shown to interact specifically with peripheral neurons and to undergo retrograde transport, was grafted to the complex core via a bifunctional PEG (HC-PEG) reactive for the thiol moieties present in the complex surface. Several formulations of HC-PEG ternary complexes were tested for targeting, by assessing the extent of cellular internalization and levels of transfection, in both the ND7/23 and NIH 3 T3 (fibroblast) cell lines. Targeted gene transfer to the neuronal cell line was observed for the complex formulations containing 5 and 7.5. Μg of HC-PEG. Finally, our results demonstrate that the developed ternary vectors are able to transfect primary cultures of dorsal root ganglion dissociated neurons in a targeted manner and elicit the expression of a relevant neurotrophic factor. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {Aberrations, animal cell, Animals, article, Bifunctional, Cell culture, Cell Line, Tumor, Cell lines, cell strain 3T3, Cells, Cultured, Cellular internalization, Chemical modification, Complex cores, Complex formulations, Complex surface, controlled study, DNA, DNA protein complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Female, Gene transfer, genetic transfection, Imines, internalization, macrogol, Mice, mouse, Multicomponents, nanoparticle, nanoparticles, neuroblastoma cell, Neuronal cell, Neurons, neurotrophic factor, Neurotrophic factors, NIH 3T3 Cells, Nitrogen compounds, Non-viral, Non-viral gene delivery, nonhuman, Nonviral, nonviral gene delivery system, Nucleic acids, Particle Size, Peptide Fragments, Peripheral nervous system, Peripheral neuropathy, Plasmids, Polyethylene Glycols, Polyethylene oxides, Polyethyleneimine, Polyethylenes, Primary culture, priority journal, process optimization, protein expression, rat, Rats, Rats, Wistar, Regenerative Medicine, Self-assembled, sensory nerve cell, spinal ganglion, Starting materials, Targeted, Targeted gene delivery, Ternary complex, Ternary vectors, Tetanus toxin, tetanus toxin fragment c, Transfection, unclassified drug, Zeta potential},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77952239026\&partnerID=40\&md5=077b03d49cf392defc3d0c77f2f249d1},
	author = {Oliveira, H. and Fernandez, R. and Pires, L.R. and Martins, M.C.L. and Sim{\~o}es, S. and Barbosa, M.A. and P{\^e}go, A.P.}
}
@article {2300,
	title = {Automated Arabidopsis plant root cell segmentation based on SVM classification and region merging},
	journal = {Computers in Biology and MedicineComput. Biol. Med.},
	volume = {39},
	year = {2009},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: CBMDAdoi: 10.1016/j.compbiomed.2009.06.008PubMed ID: 19604506Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUP, Portugal; email: marcuzzo@fe.up.ptReferences: Baeza-Yates, R., Ribeiro-Neto, B., (1999) Modern Information Retrieval, , ACM Press;Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370; 
M. Brown, W. Grundy, L.D.N. Cristianini, C. Sugnet, T. Furey, M. Ares, D. Haussler, Knowledge-based analysis of microarray gene expression data using support vector machines, in: Proceedings of the National Academy of Sciences, 97 (1), 1999, pp. 262-267Burges, C., A tutorial on support vector machines for pattern recognition (1998) Data Mining and Knowledge Discovery, 2 (2), pp. 121-167; 
Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48, pp. 619-627; 
Chang, C., Lin, C., (2001) Libsvm: A library for support vector machines, , http://www.csie.ntu.edu.tw/\~{}cjlin/libsvm/faq.html$\#$f203, Software available online at; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Transactions on Biomedical Engineering, 53 (4), pp. 762-766; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Transactions on Image Processing, 16 (8), pp. 2080-2095; 
(2004) Kernel Methods in Computational Biology, Computational Molecular Biology, , B. Sch{\"o}lkopf, K. Tsuda, J.-P. Vert, Eds, MIT Press, Cambridge, MA; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of Arabidopsis thaliana root meristem in confocal microscopy (2004) Springer Lecture Notes in Computer Science, 3212, pp. 166-174. , Proceedings of the International Conference on Image Analysis and Recognition, Springer, Berlin; 
Grandjean, O., Vernoux, T., Laufs, P., Belcram, K., Mizukami, Y., Traas, J., In vivo analysis of cell division, cell growth, and differentiation at the shoot apical meristem in Arabidopsis (2004) Plant Cell, 16 (1), pp. 74-87; 
Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in Arabidopsis thaliana provided by a simple mathematical model (2006) Journal of Plant Research, 119, pp. 85-93; 
Kittler, J., Hatef, M., Duin, R.P.W., Matas, J., On combining classifiers (1998) IEEE Transactions on Pattern Analysis and Machine Intelligence, 20 (3), pp. 226-239; 
P. Kovesi, Symmetry and asymmetry from local phase, in: Proceedings of the 10th Australian Joint Conference on Artificial Intelligence, December 1997, pp. 185-190P. Kovesi, Edges are not just steps, in: Proceedings of the Fifth Asian Conference on Computer Vision, January 2002, pp. 822-827Liao, L., Noble, W.S., Combining pairwise sequence similarity and support vector machines for remote protein homology detection (2002) Proceedings of the Sixth Annual International Conference on Computational Molecular Biology, pp. 225-232. , ACM Press; 
Luc, V., Soille, P., Watersheds in digital spaces: an efficient algorithm based on immersion simulations (1991) IEEE Transactions on Pattern Analysis and Machine Intelligence, 13 (6), pp. 583-598; 
Mao, K., Zhao, P., Tan, P.-H., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Transactions on Biomedical Engineering, 53 (6), pp. 1153-1163; 
M. Marcuzzo, P. Quelhas, A. Campilho, A.M. Mendon{\c c}a, A.C. Campilho, Automatic cell segmentation from confocal microscopy images of the Arabidopsis root, in: IEEE International Symposium on Biomedical Imaging, May 2008, pp. 712-715Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A.C., A hybrid approach for cell image segmentation (2008) Springer Lecture Notes in Computer Science, 5112, pp. 739-749. , Proceedings of the International Conference on Image Analysis and Recognition, Springer, Berlin; 
Meyer, F., Beucher, S., Morphological segmentation (1990) Journal of Visual Communication and Image Representation, 1 (1), pp. 21-46; 
Perona, P., Malik, J., Scale-space and edge detection using anisotropic diffusion (1990) IEEE Transactions on Pattern Analysis and Machine Intelligence, 7 (12), pp. 629-639; 
T. Roberts, S. Mckenna, N. Wuyts, T. Valentine, A. Bengough, Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo, in: IEEE Workshop on Motion \& Video Computing, February 2007, pp. 13-21Sathiya Keerthi, S., Lin, C.-J., Asymptotic behaviors of support vector machines with Gaussian kernel (2003) Neural Computation, 15 (7), pp. 1667-1689; 
Vapnik, V., (1998) Statistical Learning Theory, , Wiley, New York
},
	month = {2009///},
	pages = {785 - 793},
	abstract = {To obtain development information of individual plant cells, it is necessary to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Automation of cell detection/marking process is important to provide research tools in order to ease the search for special events, such as cell division. In this paper we discuss an automatic cell detection approach for Arabidopsis thaliana based on segmentation, which selects the best cell candidates from a starting watershed-based image segmentation and improves the result by merging adjacent regions. The selection of individual cells is obtained using a support vector machine (SVM) classifier, based on a cell descriptor constructed from the shape and edge strength of the cells{\textquoteright} contour. In addition we proposed a novel cell merging criterion based on edge strength along the line that connects adjacent cells{\textquoteright} centroids, which is a valuable tool in the reduction of cell over-segmentation. The result is largely pruned of badly segmented and over-segmented cells, thus facilitating the study of cells. When comparing the results after merging with the basic watershed segmentation, we obtain 1.5\% better coverage (increase in F-measure) and up to 27\% better precision in correct cell segmentation. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.},
	keywords = {accuracy, Arabidopsis, Arabidopsis plant, Arabidopsis thaliana, article, artificial intelligence, automation, Bioinformatics, Biological cells, Biomedical image processing, Cell detection, Cell divisions, Cell membranes, Cell merging, cell proliferation, Cell segmentation, Cell Shape, Cell Wall, Computer simulation, confocal microscopy, controlled study, Descriptor, F-measure, Image analysis, image processing, Image Processing, Computer-Assisted, Image segmentation, Imaging systems, In-Vivo imaging, Individual cells, Landforms, Merging, Models, Biological, nonhuman, Over segmentation, Plant cells, plant root, Plant roots, Plants (botany), priority journal, Region-merging, Research tools, root morphology, Segmented cell, signal noise ratio, support vector machine, Support vector machines, SVM classification, Watershed segmentation, Watersheds},
	isbn = {00104825 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67849119398\&partnerID=40\&md5=8029bce67aff136ccc5e0f7e4f9e0ded},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Maria Mendon{\c c}a, A. and Campilho, A.}
}
@article {2322,
	title = {Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement},
	journal = {Proceedings of the National Academy of Sciences of the United States of AmericaProc. Natl. Acad. Sci. U. S. A.},
	volume = {106},
	year = {2009},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: PNASAdoi: 10.1073/pnas.0808932106PubMed ID: 19126683Language of Original Document: EnglishCorrespondence Address: Mooney, D. J.; School of Engineering and Applied Sciences, Harvard University, 40 Oxford Street, Cambridge, MA 02138, United States; email: mooneyd@seas.harvard.eduChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; interleukin 8, 114308-91-7; vasculotropin, 127464-60-2; Hydrogels; Integrins; Interleukin-8; Vascular Endothelial Growth Factor AReferences: Debnath, J., Brugge, J.S., Modelling glandular epithelial cancers in 3-dimensional cultures (2005) Nat Rev Cancer, 5, pp. 675-688;Wang, F., Reciprocal interactions between β1-integrin and epidermal growth factor receptor in 3-dimensional basement membrane breast cultures: A different perspective in epithelial biology (1998) Proc Natl Acad Sci USA, 95, pp. 14821-14826; 
Fischbach, C., Engineering tumors with 3D scaffolds (2007) Nat Methods, 4, pp. 855-860; 
Paszek, M.J., Tensional homeostasis and the malignant phenotype (2005) Cancer Cell, 8, pp. 241-254; 
Hanahan, D., Weinberg, R.A., The hallmarks of cancer (2000) Cell, 100, pp. 57-70; 
De, S., VEGF-integrin interplay controls tumor growth and vascularization (2005) Proc Natl Acad Sci USA, 102, pp. 7589-7594; 
Lowrie, A.G., Salter, D.M., Ross, J.A., Latent effects of fibronectin, α5β1 integrin, αvβ5 integrin, and the cytoskeleton regulate pancreatic carcinoma cell IL-8 secretion (2004) Br J Cancer, 91, pp. 1327-1334; 
Cukierman, E., Pankov, R., Stevens, D.R., Yamada, K.M., Taking cell matrix adhesions to the third dimension (2001) Science, 294, pp. 1708-1712; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., Alginate hydrogels as synthetic extracellular matrix materials (1999) Biomaterials, 20, pp. 45-53; 
Lingen, M.W., Angiogenesis in the development of head and neck cancer and its inhibition by chemopreventive agents (1999) Crit Rev Oral Biol Med, 10, pp. 153-164; 
Fischbach, C., Mooney, D., Polymeric systems for bioinspired delivery of angiogenic molecules (2006) Adv Polym Sci, 203, pp. 191-221; 
Ruoslahti, E., Pierschbacher, M.D., New perspectives in cell adhesion: RGD and integrins (1987) Science, 238, pp. 491-497; 
Kong, H.J., Boontheekul, T., Mooney, D.J., Quantifying the relation between adhesion ligand-receptor bond formation and cell phenotype (2006) Proc Natl Acad Sci USA, 103, pp. 18534-18539; 
Harris, A.L., Hypoxia: A key regulatory factor in tumour growth (2002) Nat Rev Cancer, 2, pp. 38-47; 
Xu, L., Hypoxia-induced elevation in interleukin-8 expression by human ovarian carcinoma cells (1999) Cancer Res, 59, pp. 5822-5829; 
Weaver, V.M., Reversion of the malignant phenotype of human breast cells in three-dimensional cultureandin vivo by integrin-blocking antibodies (1997) J Cell Biol, 137, pp. 231-245; 
Chung, J., Hypoxia-induced vascular endothelial growth factor transcription and protection from apoptosis are dependent on α6β1 integrin in breast carcinoma cells (2004) Cancer Res, 64, pp. 4711-4716; 
Ghosh, S., Three-dimensional culture of melanoma cells profoundly affects gene expression profile: A high density oligonucleotide array study (2005) J Cell Physiol, 204, pp. 522-531; 
De Larco, J.E., A potential role for interleukin-8 in the metastatic phenotype of breast carcinoma cells (2001) Am J Pathol, 158, pp. 639-646; 
Nemeth, Z.H., Disruption of the actin cytoskeleton results in nuclear factor-κB activation and inflammatory mediator production in cultured human intestinal epithelial cells (2004) J Cell Physiol, 200, pp. 71-81; 
Xie, K., Interleukin-8 and human cancer biology (2001) Cytokine Growth Factor Rev, 12, pp. 375-391; 
Casanovas, O., Hicklin, D.J., Bergers, G., Hanahan, D., Drug resistance by evasion of antiangiogenic targeting of VEGF signaling in late-stage pancreatic islet tumors (2005) Cancer Cell, 8, pp. 299-309; 
Mizukami, Y., Induction of interleukin-8 preserves the angiogenic response in HIF-1α-deficient colon cancer cells (2005) Nat Med, 11, pp. 992-997; 
Esposito, I., Inflammatory cells contribute to the generation of an angiogenic phenotype in pancreatic ductal adenocarcinoma (2004) J Clin Pathol, 57, pp. 630-636; 
Ferrara, N., Henzel, W.J., Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells (1989) Biochem Biophys Res Commun, 161, pp. 851-858; 
Gaertner, H.F., Offord, R.E., Site-specific attachment of functionalized poly(ethylene glycol) to the amino terminus of proteins (1996) Bioconjug Chem, 7, pp. 38-44; 
Goerges, A.L., Nugent, M.A., Regulation of vascular endothelial growth factor binding and activity by extracellular pH (2003) J Biol Chem, 278, pp. 19518-19525; 
Kuschert, G.S., Identification of a glycosaminoglycan binding surface on human interleukin-8 (1998) Biochemistry, 37, pp. 11193-11201; 
Kerbel, R., Folkman, J., Clinical translation of angiogenesis inhibitors (2002) Nat Rev Cancer, 2, pp. 727-739; 
Fischbach, C., Mooney, D.J., Polymers for pro- and antiangiogenic therapy (2007) Biomaterials, 28, pp. 2069-2076; 
Alsberg, E., Engineering growing tissues (2002) Proc Natl Acad Sci USA, 99, pp. 12025-12030; 
Simmons, C.A., Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells (2004) Bone, 35, pp. 562-569; 
Comisar, W.A., Kazmers, N.H., Mooney, D.J., Linderman, J.J., Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: A combined computational and experimental approach (2007) Biomaterials, 28, pp. 4409-4417; 
Alsberg, E., Regulating bone formation via controlled scaffold degradation (2003) J Dent Res, 82, pp. 903-908; 
Richardson, T.P., Peters, M.C., Ennett, A.B., Mooney, D.J., Polymeric system for dual growth factor delivery (2001) Nat Biotechnol, 19, pp. 1029-1034
},
	month = {2009///},
	pages = {399 - 404},
	abstract = {Three-dimensional culture alters cancer cell signaling; however, the underlying mechanisms and importance of these changes on tumor vascularization remain unclear. A hydrogel system was used to examine the role of the transition from 2D to 3D culture, with and without integrin engagement, on cancer cell angiogenic capability. Three-dimensional culture recreated tumor microenvironmental cues and led to enhanced interleukin 8 (IL-8) secretion that depended on integrin engagement with adhesion peptides coupled to the polymer. In contrast, vascular endothelial growth factor (VEGF) secretion was unaffected by 3D culture with or without substrate adhesion. IL-8 diffused greater distances and was present in higher concentrations in the systemic circulation, relative to VEGF. Implantation of a polymeric IL-8 delivery system into GFP bone marrow-transplanted mice revealed that localized IL-8 up-regulation was critical to both the local and systemic control of tumor vascularization in vivo. In summary, 3D integrin engagement within tumor microenvironments regulates cancer cell angiogenic signaling, and controlled local and systemic blockade of both IL-8 and VEGF signaling may improve antiangiogenic therapies. {\textcopyright} 2009 by The National Academy of Sciences of the USA.},
	keywords = {animal experiment, animal model, Animals, arginylglycylaspartic acid, article, bone marrow transplantation, cancer cell, cancer cell culture, Cell Culture Techniques, concentration (parameters), controlled study, cytokine release, Diffusion, Drug delivery, ECM, Humans, hydrogel, Hydrogels, IL-8, implantation, in vivo study, integrin, Integrins, interleukin 8, Interleukin-8, Mice, microenvironment, Models, Biological, mouse, Mus, Neoplasms, Neovascularization, Pathologic, nonhuman, polymer, priority journal, signal transduction, systemic circulation, Tumor vascularization, upregulation, Vascular Endothelial Growth Factor A, vasculotropin},
	isbn = {00278424 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849121351\&partnerID=40\&md5=acc07042d6f5801f7b704a3f355917cf},
	author = {Fischbach, C. and Hyun, J.K. and Hsiong, S.X. and Evangelista, M.B. and Yuen, W. and Mooney, D.J.}
}
@article {2307,
	title = {Cancer invasion and metastasis: Interacting ecosystems},
	journal = {Virchows ArchivVirchows Arch.},
	volume = {454},
	year = {2009},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: VARCEdoi: 10.1007/s00428-009-0784-0PubMed ID: 19471961Language of Original Document: EnglishCorrespondence Address: Madani, I.; Department of Radiotherapy, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium; email: indira.madani@ugent.beReferences: Tarin, D., New insights into the pathogenesis of breast cancer metastasis (2006) Breast Disease, 26 (1), pp. 13-25;Stafford, L.J., Vaidya, K.S., Welch, D.R., Metastasis suppressors genes in cancer (2008) Int J Biochem Cell Biol, 40, pp. 874-891; 
Oliveira, M.J., (2004) The Effect of Bacteria on Colon Cancer Cell Invasion: Molecular Mechanisms Associated, , Faculty of Medicine and Health Sciences. Ghent University. PhD thesis; 
Madani, I., De Neve, W., Mareel, M., Does ionizing radiation stimulate cancer invasion and metastasis? (2008) Bull Cancer, 95, pp. 292-300; 
De Wever, O., Mareel, M., Role of tissue stroma in cancer cell invasion (2003) Journal of Pathology, 200 (4), pp. 429-447. , DOI 10.1002/path.1398; 
Bracke, M.E., Vanhoecke, B.W.A., Derycke, L., Bolca, S., Possemiers, S., Heyerick, A., Stevens, C.V., Parmar, V.S., Plant polyphenolics as anti-invasive cancer agents (2008) Anti-Cancer Agents in Medicinal Chemistry, 8 (2), pp. 171-185. , http://www.ingentaconnect.com/content/ben/acamc/2008/00000008/00000002/ art00004, DOI 10.2174/187152008783497037; 
Ottewell, P.D., Coleman, R.E., Holen, I., From genetic abnormality to metastases: Murine models of breast cancer and their use in the development of anticancer therapies (2006) Breast Cancer Res Treat, 96, pp. 101-113; 
Langley, R.R., Fidler, I.J., Tumor cell-organ microenvironment interactions in the pathogenesis of cancer metastasis (2007) Endocrine Reviews, 28 (3), pp. 297-321. , http://edrv.endojournals.org/cgi/reprint/28/3/297.pdf, DOI 10.1210/er.2006-0027; 
Kuperwasser, C., Chavarria, T., Wu, M., Magrane, G., Gray, J.W., Carey, L., Richardson, A., Weinberg, R.A., Reconstruction of functionally normal and malignant human breast tissues in mice (2004) Proceedings of the National Academy of Sciences of the United States of America, 101 (14), pp. 4966-4971. , DOI 10.1073/pnas.0401064101; 
Hoffman, R.M., Real-time subcellular imaging in live animals: New visible targets for cancer drug discovery (2006) IDrugs, 9 (9), pp. 632-635; 
Fomchenko, E.I., Holland, E.C., Mouse models of brain tumors and their applications in preclinical trials (2006) Clinical Cancer Research, 12 (18), pp. 5288-5297. , DOI 10.1158/1078-0432.CCR-06-0438; 
Gingrich, J.R., Barrios, R.J., Morton, R.A., Boyce, B.F., DeMayo, F.J., Finegold, M.J., Angelopoulou, R., Greenberg, N.M., Metastatic prostate cancer in a transgenic mouse (1996) Cancer Research, 56 (18), pp. 4096-4102; 
Peri, A.-K., Wilgenbus, P., Dahl, U., Semb, H., Christofori, G., A causal role for E-cadherin in the transition from adenoma to carcinoma (1998) Nature, 392 (6672), pp. 190-193. , DOI 10.1038/32433; 
Ambartsumian, N., Grigorian, M., Lukanidin, E., Genetically modified mouse models to study the role of metastasis-promoting S100A4(mts1) protein in metastatic mammary cancer (2005) Journal of Dairy Research, 72 (SPEC. ISS.), pp. 27-33. , DOI 10.1017/S0022029905001093; 
Scherer, R.L., McIntyre, J.O., Matrisian, L.M., Imaging matrix metalloproteinases in cancer (2008) Cancer Metastasis Rev, 27, pp. 679-690; 
Espina, V., Heiby, M., Pierobon, M., Liotta, L.A., Laser capture microdissection technology (2007) Expert Review of Molecular Diagnostics, 7 (5), pp. 647-657. , http://www.future-drugs.com/doi/pdf/10.1586/14737159.7.5.647, DOI 10.1586/14737159.7.5.647; 
Eccles, S.A., Welch, D.R., Metastasis: recent discoveries and novel treatment strategies (2007) Lancet, 369 (9574), pp. 1742-1757. , DOI 10.1016/S0140-6736(07)60781-8, PII S0140673607607818; 
Brenton, J.D., Carey, L.A., Ahmed, A., Caldas, C., Molecular classification and molecular forecasting of breast cancer: Ready for clinical application? (2005) Journal of Clinical Oncology, 23 (29), pp. 7350-7360. , DOI 10.1200/JCO.2005.03.3845; 
Montel, V., Huang, T.-Y., Mose, E., Pestonjamasp, K., Tarin, D., Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model (2005) American Journal of Pathology, 166 (5), pp. 1565-1579; 
Karpf, A.R., Jones, D.A., Reactivating the expression of methylation silenced genes in human cancer (2002) Oncogene, 21 (35 REV. ISS. 3), pp. 5496-5503. , DOI 10.1038/sj.onc.1205602; 
Feng, Y., Sun, B., Li, X., Zhang, L., Niu, Y., Xiao, C., Ning, L., Hao, X., Differentially expressed genes between primary cancer and paired lymph node metastases predict clinical outcome of node-positive breast cancer patients (2007) Breast Cancer Research and Treatment, 103 (3), pp. 319-329. , DOI 10.1007/s10549-006-9385-7; 
Van{\textquoteright}t Veer, L.J., Dai, H., Van De Vijver, M.J., He, Y.D., Hart, A.A.M., Mao, M., Peterse, H.L., Friend, S.H., Gene expression profiling predicts clinical outcome of breast cancer (2002) Nature, 415 (6871), pp. 530-536. , DOI 10.1038/415530a; 
Wang, W., Klijn, J.G., Zhang, Y., Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer (2005) Lancet, 415, pp. 530-536; 
Eckhardt, B.L., Parker, B.S., Van Laar, R.K., Restall, C.M., Natoli, A.L., Tavaria, M.D., Stanley, K.L., Anderson, R.L., Genomic analysis of a spontaneous model of breast cancer metastasis to bone reveals a role for the extracellular matrix (2005) Molecular Cancer Research, 3 (1), pp. 1-13; 
Adib, T.R., Henderson, S., Perrett, C., Hewitt, D., Bourmpoulia, D., Ledermann, J., Boshoff, C., Predicting biomarkers for ovarian cancer using gene-expression microarrays (2004) British Journal of Cancer, 90 (3), pp. 686-692. , DOI 10.1038/sj.bjc.6601603; 
Suzuki, M., Tarin, D., Gene expression profiling of human lymph node metastases and matched primary breast carcinomas: Clinical implications (2007) Molecular Oncology, 1 (2), pp. 172-180. , DOI 10.1016/j.molonc.2007.03.005, PII S1574789107000154; 
Chang, H.Y., Nuyten, D.S.A., Sneddon, J.B., Hastie, T., Tibshirani, R., Sorlie, T., Dai, H., Van De Vijver, M.J., Robustness, scalability, and integration of a wound-response gene expression signature in predicting breast cancer survival (2005) Proceedings of the National Academy of Sciences of the United States of America, 102 (10), pp. 3738-3743. , DOI 10.1073/pnas.0409462102; 
Finak, G., Bertos, N., Pepin, F., Sadekova, S., Souleimanova, M., Zhao, H., Chen, H., Park, M., Stromal gene expression predicts clinical outcome in breast cancer (2008) Nature Medicine, 14 (5), pp. 518-527. , DOI 10.1038/nm1764, PII NM1764; 
Clark, I.M., Swingler, T.E., Sampieri, C.L., The regulation of matrix metalloproteinases and their inhibitors (2008) Int J Biochem Cell Biol, 40, pp. 1362-1378; 
Li, Y., Liang, J., Kang, S., Dong, Z., Wang, N., Xing, H., Zhou, R., Zhao, X., E-cadherin gene polymorphisms and haplotype associated with the occurrence of epithelial ovarian cancer in Chinese (2008) Gynecologic Oncology, 108 (2), pp. 409-414. , DOI 10.1016/j.ygyno.2007.10.024, PII S0090825807008517; 
Brendle, A., Lei, H., Brandt, A., Polymorphisms in predicted microRNA-binding sites in integrin genes and breast cancer: ITGB4 as prognostic marker (2008) Carcinogenesis, 29, pp. 1394-1399; 
Hunter, K.W., Crawford, N.P., Germ line polymorphism in metastatic progression (2006) Cancer Research, 66 (3), pp. 1251-1254. , DOI 10.1158/0008-5472.CAN-05-3705; 
Crawford, N.P., Ziogas, A., Peel, D.J., Germline polymorphisms in SIPA1 are associated with metastasis and other indicators of poor prognosis in breast cancer (2006) Breast Cancer Res, 8, p. 16; 
Van Roy, F., Berx, G., The cell-cell adhesion molecule E-cadherin (2008) Cell Mol Life Sci, 65, pp. 3756-3788; 
Bartel, D.P., Chen, C.-Z., Micromanagers of gene expression: The potentially widespread influence of metazoan microRNAs (2004) Nature Reviews Genetics, 5 (5), pp. 396-400; 
Ma, L., Weinberg, R.A., Micromanagers of malignancy: Role of microRNAs in regulating metastasis (2008) Trends Genet, 24, pp. 448-456; 
Graff, J.R., Zimmer, S.G., Translational control and metastatic progression: Enhanced activity of the mRNA cap-binding protein eIF-4E selectively enhances translation of metastasis-related mRNAs (2003) Clinical and Experimental Metastasis, 20 (3), pp. 265-273. , DOI 10.1023/A:1022943419011; 
Hao, X., Sun, B., Hu, L., Lahdesmaki, H., Dunmire, V., Feng, Y., Zhang, S.-W., Zhang, W., Differential Gene and Protein Expression in Primary Breast Malignancies and Their Lymph Node Metastases as Revealed by Combined cDNA Microarray and Tissue Microarray Analysis (2004) Cancer, 100 (6), pp. 1110-1122. , DOI 10.1002/cncr.20095; 
Hakomori, S.-I., Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism (1996) Cancer Research, 56 (23), pp. 5309-5318; 
Yoshimura, M., Ihara, Y., Matsuzawa, Y., Taniguchi, N., Aberrant glycosylation of E-cadherin enhances cell-cell binding to suppress metastasis (1996) Journal of Biological Chemistry, 271 (23), pp. 13811-13815. , DOI 10.1074/jbc.271.23.13811; 
McLachlan, R.W., Yap, A.S., Not so simple: The complexity of phosphotyrosine signaling at cadherin adhesive contacts (2007) Journal of Molecular Medicine, 85 (6), pp. 545-554. , DOI 10.1007/s00109-007-0198-x, Special Topic: Tumor Cell Invasion and Metastasis; 
Wipff, P.J., Hinz, B., Integrins and the activation of latent transforming growth factor beta1-an intimate relationship (2008) Eur J Cell Biol, 87, pp. 601-615; 
Paredes, J., Correia, A.L., Ribeiro, A.S., P-cadherin expression in breast cancer: A review (2007) Breast Cancer Res, 9, p. 214; 
Van Marck, V., Stove, C., Van Den Bossche, K., Stove, V., Paredes, J., Vander Haeghen, Y., Bracke, M., P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma (2005) Cancer Research, 65 (19), pp. 8774-8783. , DOI 10.1158/0008-5472.CAN-04-4414; 
Wheelock, M.J., Shintani, Y., Maeda, M., Fukumoto, Y., Johnson, K.R., Cadherin switching (2008) Journal of Cell Science, 121 (6), pp. 727-735. , DOI 10.1242/jcs.000455; 
De Wever, O., Westbroek, W., Verloes, A., Bloemen, N., Bracke, M., Gespach, C., Bruyneel, E., Mareel, M., Critical role of N-cadherin in myofibroblast invasion and migration in vitro stimulated by colon-cancer-cell-derived TGF-β or wounding (2004) Journal of Cell Science, 117 (20), pp. 4691-4703. , DOI 10.1242/jcs.01322; 
Alexander, N.R., Tran, N.L., Rekapally, H., N-cadherin gene expression in prostate carcinoma is modulated by integrin-dependent nuclear translocation of Twist1 (2006) Cancer Res, 66, pp. 3365-3369; 
Stove, C., Bracke, M., Roles for neuregulins in human cancer (2005) Clinical and Experimental Metastasis, 21 (8), pp. 665-684. , DOI 10.1007/s10585-004-6917-6; 
Kerbel, R.S., Tumor angiogenesis (2008) New England Journal of Medicine, 358 (19), pp. 2039-2049. , http://content.nejm.org/cgi/reprint/358/19/2039.pdf, DOI 10.1056/NEJMra0706596; 
Chabottaux, V., Noel, A., Breast cancer progression: Insights into multifaceted matrix metalloproteinases (2007) Clinical and Experimental Metastasis, 24 (8), pp. 647-656. , DOI 10.1007/s10585-007-9113-7; 
Noel, A., Jost, M., Maquoi, E., Matrix metalloproteinases at cancer tumor-host interface (2008) Seminars in Cell and Developmental Biology, 19 (1), pp. 52-60. , DOI 10.1016/j.semcdb.2007.05.011, PII S1084952107000821, Matrix Metalloproteinases; 
Jones, R.B., Gordus, A., Krall, J.A., MacBeath, G., A quantitative protein interaction network for the ErbB receptors using protein microarrays (2006) Nature, 439 (7073), pp. 168-174. , DOI 10.1038/nature04177, PII NATURE04177; 
Pawson, T., Nash, P., Assembly of cell regulatory systems through protein interaction domains (2003) Science, 300 (5618), pp. 445-452. , DOI 10.1126/science.1083653; 
Kopfstein, L., Christofori, G., Metastasis: Cell-autonomous mechanisms versus contributions by the tumor microenvironment (2006) Cell Mol Life Sci, 63, pp. 449-468; 
Paget, S., The distribution of secondary growths in cancer of the breast (1889) Lancet, 1, pp. 571-573; 
Mareel, M.M., Behrens, J., Birchmeier, W., Down-regulation of E-cadherin expression in Madin Darby canine kidney (MDCK) cells inside tumors of nude mice (1991) Int J Cancer, 47, pp. 922-928; 
Creighton, C.J., Bromberg-White, J.L., Misek, D.E., Monsma, D.J., Brichory, F., Kuick, R., Giordano, T.J., Hanash, S.M., Analysis of tumor-host interactions by gene expression profiling of lung adenocarcinoma xenografts identifies genes involved in tumor formation (2005) Molecular Cancer Research, 3 (3), pp. 119-129. , DOI 10.1158/1541-7786.MCR-04-0189; 
Dimanche-Boitrel, M.T., Vakaet Jr., L., Pujuguet, P., Chauffert, B., Martin, M.S., Hammann, A., Van Roy, F., Martin, F., In vivo and in vitro invasiveness of a rat colon-cancer cell line maintaining E-cadherin expression: An enhancing role of tumor-associated myofibroblasts (1994) International Journal of Cancer, 56 (4), pp. 512-521; 
Lin, E.Y., Pollard, J.W., Tumor-associated macrophages press the angiogenic switch in breast cancer (2007) Cancer Research, 67 (11), pp. 5064-5066. , http://cancerres.aacrjournals.org/cgi/reprint/67/11/5064, DOI 10.1158/0008-5472.CAN-07-0912; 
Korc, M., Pancreatic cancer-associated stroma production (2007) Am J sur, 194 (4 SUPPL.), pp. 84-S86; 
He, Y., Rajantie, I., Pajusola, K., Jeltsch, M., Holopainen, T., Yla-Herttuala, S., Harding, T., Alitalo, K., Vascular endothelial cell growth factor receptor 3-mediated activation of lymphatic endothelium is crucial for tumor cell entry and spread via lymphatic vessels (2005) Cancer Research, 65 (11), pp. 4739-4746. , DOI 10.1158/0008-5472.CAN-04-4576; 
Mahabeleshwar, G.H., Byzova, T.V., Angiogenesis in Melanoma (2007) Seminars in Oncology, 34 (6), pp. 555-565. , DOI 10.1053/j.seminoncol.2007.09.009, PII S0093775407001832, Malignant Melanoma; 
Benoy, I.H., Salgado, R., Elst, H., Relative microvessel area of the primary tumour, and not lymph node status, predicts the presence of bone marrow micrometastases detected by reverse transcriptase polymerase chain reaction in patients with clinically non-metastatic breast cancer (2005) Breast Cancer Res, 7, pp. 210-R219; 
Hicklin, D.J., Ellis, L.M., Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis (2005) Journal of Clinical Oncology, 23 (5), pp. 1011-1027. , DOI 10.1200/JCO.2005.06.081; 
Schoppmann, S.F., Bayer, G., Aumayr, K., Taucher, S., Geleff, S., Rudas, M., Kubista, E., Horvat, R., Prognostic value of lymphangiogenesis and lymphovascular invasion in invasive breast cancer (2004) Annals of Surgery, 240 (2), pp. 306-312. , DOI 10.1097/01.sla.0000133355.48672.22; 
Tobler, N.E., Detmar, M., Tumor and lymph node lymphangiogenesis - Impact on cancer metastasis (2006) Journal of Leukocyte Biology, 80 (4), pp. 691-696. , http://www.jleukbio.org/cgi/reprint/80/4/691, DOI 10.1189/jlb.1105653; 
Nathanson, S.D., Leong, S., Preclinical models of regional lymph node tumor metastasis (2007) Cancer Metastasis and the Lymphvascular System: Basis for Rational Therapy, pp. 129-156. , Springer New York; 
Wissmann, C., Detmar, M., Pathways targeting tumor lymphangiogenesis (2006) Clinical Cancer Research, 12 (23), pp. 6865-6868. , DOI 10.1158/1078-0432.CCR-06-1800; 
Rafii, D.C., Psaila, B., Butler, J., Jin, D.K., Lyden, D., Regulation of vasculogenesis by platelet-mediated recruitment of bone marrow-derived cells (2008) Arteriosclerosis, Thrombosis, and Vascular Biology, 28 (2), pp. 217-222. , DOI 10.1161/ATVBAHA.107.151159, PII 0004360520080200000004; 
Mantovani, A., Sica, A., Sozzani, S., Allavena, P., Vecchi, A., Locati, M., The chemokine system in diverse forms of macrophage activation and polarization (2004) Trends in Immunology, 25 (12), pp. 677-686. , DOI 10.1016/j.it.2004.09.015, PII S1471490604002959; 
Mantovani, A., Romero, P., Palucka, A.K., Tumour immunity: Effector response to tumour and role of the microenvironment (2008) Lancet, 371, pp. 771-783; 
Talmadge, J.E., Donkor, M., Scholar, E., Inflammatory cell infiltration of tumors: Jekyll or Hyde (2007) Cancer and Metastasis Reviews, 26 (3-4), pp. 373-400. , DOI 10.1007/s10555-007-9072-0, Forty Years of Metastasis Research: A Tribute to Dr. Isaiah J. Fidler; 
Lewis, C.E., Pollard, J.W., Distinct role of macrophages in different tumor microenvironments (2006) Cancer Research, 66 (2), pp. 605-612. , DOI 10.1158/0008-5472.CAN-05-4005; 
Condeelis, J., Pollard, J.W., Macrophages: Obligate partners for tumor cell migration, invasion, and metastasis (2006) Cell, 124 (2), pp. 263-266. , DOI 10.1016/j.cell.2006.01.007, PII S0092867406000559; 
Wyckoff, J.B., Wang, Y., Lin, E.Y., Li, J.-F., Goswami, S., Stanley, E.R., Segall, J.E., Condeelis, J., Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors (2007) Cancer Research, 67 (6), pp. 2649-2656. , DOI 10.1158/0008-5472.CAN-06-1823; 
Mahadevan, D., Von Hoff, D.D., Tumor-stroma interactions in pancreatic ductal adenocarcinoma (2007) Molecular Cancer Therapeutics, 6 (4), pp. 1186-1197. , DOI 10.1158/1535-7163.MCT-06-0686; 
De Wever, O., Demetter, P., Mareel, M., Stromal myofibroblasts are drivers of invasive cancer growth (2008) Int J Cancer, 123, pp. 2229-2238; 
Massagu{\`e}, J., TGFbeta in cancer (2008) Cell, 134, pp. 215-230; 
Hu, M., Yao, J., Carroll, D.K., Weremowicz, S., Chen, H., Carrasco, D., Richardson, A., Polyak, K., Regulation of In Situ to Invasive Breast Carcinoma Transition (2008) Cancer Cell, 13 (5), pp. 394-406. , DOI 10.1016/j.ccr.2008.03.007, PII S1535610808000913; 
Lerwill, M.F., Current practical applications of diagnostic immunohistochemistry in breast pathology (2004) American Journal of Surgical Pathology, 28 (8), pp. 1076-1091. , DOI 10.1097/01.pas.0000126780.10029.f0; 
Allinen, M., Beroukhim, R., Cai, L., Brennan, C., Lahti-Domenici, J., Huang, H., Porter, D., Polyak, K., Molecular characterization of the tumor microenvironment in breast cancer (2004) Cancer Cell, 6 (1), pp. 17-32. , DOI 10.1016/j.ccr.2004.06.010, PII S1535610804001783; 
Hu, M., Yao, J., Cai, L., Bachman, K.E., Van Den Brule, F., Velculescu, V., Polyak, K., Distinct epigenetic changes in the stromal cells of breast cancers (2005) Nature Genetics, 37 (8), pp. 899-905. , DOI 10.1038/ng1596; 
Barsky, S.H., Karlin, N.J., Myoepithelial cells: Autocrine and paracrine suppressors of breast cancer progression (2005) J Mammary Gland Biol Neoplasia, 10, pp. 249-260; 
Polyak, K., Hu, M., Do myoepithelial cells hold the key for breast tumor progression? (2005) J Mammary Gland Biol Neoplasia, 10, pp. 231-247; 
Mantyh, P.W., Clohisy, D.R., Koltzenburg, M., Hunt, S.P., Molecular mechanisms of cancer pain (2002) Nature Reviews Cancer, 2 (3), pp. 201-209; 
Ayala, G.E., Dai, H., Powell, M., Cancer-related axonogenesis and neurogenesis in prostate cancer (2008) Clin Cancer Res, 14, pp. 7593-7603; 
Wang, W., Zhao, H., Zhang, S., Al, E., Patterns of expression and function of the p75(NGFR) protein in pancreatic cancer cells and tumours (2008) Eur J Surg Oncol, , Nov 26 (in press); 
Marchesi, F., Piemonti, L., Fedele, G., The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma (2008) Cancer Res, 68, pp. 9060-9069; 
Zhang, S., Qi, L., Li, M., Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer (2008) J Exp Clin Cancer Res, 27, p. 62; 
Andarawewa, K.L., Motrescu, E.R., Chenard, M.-P., Gansmuller, A., Stoll, I., Tomasetto, C., Rio, M.-C., Stromelysin-3 is a potent negative regulator of adipogenesis participating to cancer cell-adipocyte interaction/crosstalk at the tumor invasive front (2005) Cancer Research, 65 (23), pp. 10862-10871. , DOI 10.1158/0008-5472.CAN-05-1231; 
Caers, J., Van Valckenborgh, E., Menu, E., Unraveling the biology of multiple myeloma disease: Cancer stem cells, acquired intracellular changes and interactions with the surrounding micro-environment (2008) Bull Cancer, 95, pp. 301-313; 
Vona-Davis, L., Rose, D.P., Adipokines as endocrine, paracrine, and autocrine factors in breast cancer risk and progression (2007) Endocrine-Related Cancer, 14 (2), pp. 189-206. , DOI 10.1677/ERC-06-0068; 
Kunduzova, O., Alet, N., Delesque-Touchard, N., Apelin/APJ signaling system: A potential link between adipose tissue and endothelial angiogenic processes (2008) FASEB J, 22, pp. 4146-4153; 
Cirillo, D., Rachiglio, A.M., La Montagna, R., Leptin signaling in breast cancer: An overview (2008) J Cell Biochem, 105, pp. 956-964; 
Jaffe, T., Schwartz, B., Leptin promotes motility and invasiveness in human colon cancer cells by activating multiple signal-transduction pathways (2008) Int J Cancer, 123, pp. 2543-2556; 
Horiguchi, A., Sumitomo, M., Asakuma, J., Asano, T., Zheng, R., Asano, T., Nanus, D.M., Hayakawa, M., Leptin Promotes Invasiveness of Murine Renal Cancer Cells Via Extracellular Signal-Regulated Kinases and Rho Dependent Pathway (2006) Journal of Urology, 176 (4), pp. 1636-1641. , DOI 10.1016/j.juro.2006.06.040, PII S0022534706014169; 
Bussard, K.M., Gay, C.V., Mastro, A.M., The bone microenvironment in metastasis; What is special about bone? (2008) Cancer Metastasis Rev, 27, pp. 41-55; 
Vakaet, L.A.M.-L., Boterberg, T., Pain control by ionizing radiation of bone metastasis (2004) International Journal of Developmental Biology, 48 (5-6), pp. 599-606. , DOI 10.1387/ijdb.041817lv, Invasion in Cancer and Embryonic Development; 
Oliveira, M.J., Van Damme, J., Lauwaet, T., De Corte, V., De Bruyne, G., Verschraegen, G., Vaneechoutte, M., Leroy, A., β-casein-derived peptides, produced by bacteria, stimulate cancer cell invasion and motility (2003) EMBO Journal, 22 (22), pp. 6161-6173. , DOI 10.1093/emboj/cdg586; 
Oliveira, M.J., Costa, A.C., Costa, A.M., Henriques, L., Suriano, G., Atherton, J.C., Machado, J.C., Figueiredo, C., Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system-dependent manner (2006) Journal of Biological Chemistry, 281 (46), pp. 34888-34896. , http://www.jbc.org/cgi/reprint/281/46/34888, DOI 10.1074/jbc.M607067200; 
Oliveira, M.J., Costa, A.M., Costa, A.C., Al, E., CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell invasive phenotype (2009) J Infect Dis, , in press; 
Miller, M.J., Wei, S.H., Parker, I., Cahalan, M.D., Two-photon imaging of lymphocyte motility and antigen response in intact lymph node (2002) Science, 296 (5574), pp. 1869-1873. , DOI 10.1126/science.1070051; 
Mullen, J.T., Wang, H., Yao, J.C., Carcinoid tumors of the duodenum (2005) Surgery, 138, pp. 971-977; 
Hoon, D.S.B., Kitago, M., Kim, J., Mori, T., Piris, A., Szyfelbein, K., Mihm Jr., M.C., Pantel, K., Molecular mechanisms of metastasis (2006) Cancer and Metastasis Reviews, 25 (2), pp. 203-220. , DOI 10.1007/s10555-006-8500-x; 
Muller, A., Homey, B., Soto, H., Ge, N., Catron, D., Buchanan, M.E., McClanahan, T., Zlotnik, A., Involvement of chemokine receptors in breast cancer metastasis (2001) Nature, 410 (6824), pp. 50-56. , DOI 10.1038/35065016; 
Hirakawa, S., Brown, L.F., Kodama, S., Paavonen, K., Alitalo, K., Detmar, M., VEGF-C-induced lymphangiogenesis in sentinel lymph nodes promotes tumor metastasis to distant sites (2007) Blood, 109 (3), pp. 1010-1017. , http://www.bloodjournal.org/cgi/reprint/109/3/1010, DOI 10.1182/blood-2006-05-021758; 
Van Den Eynden, G.G., Vandenberghe, M.K., Van Dam, P.-J.H., Colpaert, C.G., Van Dam, P., Dirix, L.Y., Vermeulen, P.B., Van Marck, E.A., Increased sentinel lymph node lymphangiogenesis is associated with nonsentinel axillary lymph node involvement in breast cancer patients with a positive sentinel node (2007) Clinical Cancer Research, 13 (18), pp. 5391-5397. , http://clincancerres.aacrjournals.org/cgi/reprint/13/18/5391, DOI 10.1158/1078-0432.CCR-07-1230; 
Dimeo, T.A., Kuperwasser, C., The evolving paradigm of tissue-specific metastasis (2006) Breast Cancer Res, 8, p. 301; 
Andre, F., Cabioglu, N., Assi, H., Sabourin, J.C., Delaloge, S., Sahin, A., Broglio, K., Cristofanilli, M., Expression of chemokine receptors predicts the site of metastatic relapse in patients with axillary node positive primary breast cancer (2006) Annals of Oncology, 17 (6), pp. 945-951. , DOI 10.1093/annonc/mdl053; 
Stessels, F., Van Den Eynden, G., Van Der Auwera, I., Salgado, R., Van Den Heuvel, E., Harris, A.L., Jackson, D.G., Vermeulen, P.B., Breast adenocarcinoma liver metastases, in contrast to colorectal cancer liver metastases, display a non-angiogenic growth pattern that preserves the stroma and lacks hypoxia (2004) British Journal of Cancer, 90 (7), pp. 1429-1436. , DOI 10.1038/sj.bjc.6601727; 
Mendes, O., Kim, H.-T., Lungu, G., Stoica, G., MMP2 role in breast cancer brain metastasis development and its regulation by TIMP2 and ERK1/2 (2007) Clinical and Experimental Metastasis, 24 (5), pp. 341-351. , DOI 10.1007/s10585-007-9071-0; 
Kaplan, R.N., Psaila, B., Lyden, D., Bone marrow cells in the {\textquoteright}pre-metastatic niche{\textquoteright}: Within bone and beyond (2006) Cancer and Metastasis Reviews, 25 (4), pp. 521-529. , DOI 10.1007/s10555-006-9036-9, Special issue on Bone Metastasis and Cancer; 
Hiratsuka, S., Watanabe, A., Aburatani, H., Maru, Y., Tumour-mediated upregulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis (2006) Nature Cell Biology, 8 (12), pp. 1369-1375. , DOI 10.1038/ncb1507, PII NCB1507; 
Kaplan, R.N., Psaila, B., Lyden, D., Niche-to-niche migration of bone-marrow-derived cells (2007) Trends in Molecular Medicine, 13 (2), pp. 72-81. , DOI 10.1016/j.molmed.2006.12.003, PII S1471491406002681; 
Pantel, K., Brakenhoff, R.H., Dissecting the metastatic cascade (2004) Nature Reviews Cancer, 4 (6), pp. 448-456; 
Vincent-Salomon, A., Bidard, F.C., Pierga, J.Y., Bone marrow micrometastasis in breast cancer: Review of detection methods, prognostic impact and biological issues (2008) Journal of Clinical Pathology, 61 (5), pp. 570-576. , DOI 10.1136/jcp.2007.046649; 
Bidard, F.C., Vincent-Salomon, A., Gomme, S., Disseminated tumor cells of breast cancer patients: A strong prognostic factor for distant and local relapse (2008) Clin Cancer Res, 14, pp. 3306-3311. , Institut Curie Breast Cancer Study Group et al; 
Ceelen, W.P., Bracke, M.E., Peritoneal minimal residual disease in colorectal cancer: Mechanisms, prevention, and treatment (2009) Lancet Oncol, 10, pp. 72-79; 
Bidard, F.C., Pierga, J.Y., Vincent-Salomon, A., A "class action" against the microenvironment: Do cancer cells cooperate in metastasis? (2008) Cancer Metastasis Rev, 27, pp. 5-10; 
Bertolini, F., Mancuso, P., Shaked, Y., Kerbel, R.S., Molecular and cellular biomarkers for angiogenesis in clinical oncology (2007) Drug Discovery Today, 12 (19-20), pp. 806-812. , DOI 10.1016/j.drudis.2007.08.011, PII S1359644607003534; 
Shields, J.D., Fleury, M.E., Yong, C., Tomei, A.A., Randolph, G.J., Swartz, M.A., Autologous Chemotaxis as a Mechanism of Tumor Cell Homing to Lymphatics via Interstitial Flow and Autocrine CCR7 Signaling (2007) Cancer Cell, 11 (6), pp. 526-538. , DOI 10.1016/j.ccr.2007.04.020, PII S1535610807001456; 
Nierodzik, M.L., Karpatkin, S., Thrombin induces tumor growth, metastasis, and angiogenesis: Evidence for a thrombin-regulated dormant tumor phenotype (2006) Cancer Cell, 10 (5), pp. 355-362. , DOI 10.1016/j.ccr.2006.10.002, PII S1535610806002868; 
Podsypanina, K., Du, Y.C., Jechlinger, M., Seeding and propagation of untransformed mouse mammary cells in the lung (2008) Science, 321, pp. 1841-1844; 
Aguirre-Ghiso, J.A., Models, mechanisms and clinical evidence for cancer dormancy (2007) Nature Reviews Cancer, 7 (11), pp. 834-846. , DOI 10.1038/nrc2256, PII NRC2256; 
Brackstone, M., Townson, J.L., Chambers, A.F., Tumour dormancy in breast cancer: An update (2007) Breast Cancer Res, 9, p. 208; 
O{\textquoteright}Reilly, M.S., Holmgren, L., Shing, Y., Chen, C., Rosenthal, R.A., Moses, M., Lane, W.S., Folkman, J., Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma (1994) Cell, 79 (2), pp. 315-328. , DOI 10.1016/0092-8674(94)90200-3; 
Norton, L., Massague, J., Is cancer a disease of self-seeding? (2006) Nature Medicine, 12 (8), pp. 875-878. , DOI 10.1038/nm0806-875, PII NM0806875; 
Bidard, F.C., (2008) Interactions en Coop{\'e}Rations Cellulaires dans le Processus M{\'e}tastatique, , MSc thesis. L{\textquoteright}Universit{\'e} Paris XI; 
Benvenuti, S., Comoglio, P.M., The MET receptor tyrosine kinase in invasion and metastasis (2007) Journal of Cellular Physiology, 213 (2), pp. 316-325. , DOI 10.1002/jcp.21183; 
Mareel, M., Leroy, A., Clinical, cellular, and molecular aspects of cancer invasion (2003) Physiological Reviews, 83 (2), pp. 337-376; 
Christofori, G., New signals from the invasive front (2006) Nature, 441 (7092), pp. 444-450. , DOI 10.1038/nature04872, PII NATURE04872; 
Sabbah, M., Emami, S., Redeuilh, G., Molecular signature and therapeutic perspective of the epithelial-to-mesenchymal transitions in epithelial cancers (2008) Drug Resist Updat, 11, pp. 123-151; 
Thiery, J.P., Sleeman, J.P., Complex networks orchestrate epithelial-mesenchymal transitions (2006) Nature Reviews Molecular Cell Biology, 7 (2), pp. 131-142. , DOI 10.1038/nrm1835, PII NRM1835; 
Yang, J., Weinberg, R.A., Epithelial-Mesenchymal Transition: At the Crossroads of Development and Tumor Metastasis (2008) Developmental Cell, 14 (6), pp. 818-829. , DOI 10.1016/j.devcel.2008.05.009, PII S1534580708002098; 
Christiansen, J.J., Rajasekaran, A.K., Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis (2006) Cancer Research, 66 (17), pp. 8319-8326. , DOI 10.1158/0008-5472.CAN-06-0410; 
Brabletz, T., Jung, A., Spaderna, S., Hlubek, F., Kirchner, T., Migrating cancer stem cells - An integrated concept of malignant tumour progression (2005) Nature Reviews Cancer, 5 (9), pp. 744-749. , DOI 10.1038/nrc1694, PII N1694; 
Tarin, D., The fallacy of epithelial mesenchymal transition in neoplasia (2005) Cancer Research, 65 (14), pp. 5996-6000. , DOI 10.1158/0008-5472.CAN-05-0699; 
Garber, K., Epithelial-to-mesenchymal transition is important to metastasis, but questions remain (2008) J Natl Cancer Inst, 100, pp. 232-239; 
De Wever, O., Derycke, L., Hendrix, A., De Meerleer, G., Godeau, F., Depypere, H., Bracke, M., Soluble cadherins as cancer biomarkers (2007) Clinical and Experimental Metastasis, 24 (8), pp. 685-697. , DOI 10.1007/s10585-007-9104-8; 
Bellahcene, A., Castronovo, V., Ogbureke, K.U.E., Fisher, L.W., Fedarko, N.S., Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): Multifunctional proteins in cancer (2008) Nature Reviews Cancer, 8 (3), pp. 212-226. , DOI 10.1038/nrc2345, PII NRC2345; 
Gimona, M., Buccione, R., Adhesions that mediate invasion (2006) International Journal of Biochemistry and Cell Biology, 38 (11), pp. 1875-1892. , DOI 10.1016/j.biocel.2006.05.003, PII S1357272506001580; 
Gimona, M., Buccione, R., Courtneidge, S.A., Assembly and biological role of podosomes and invadopodia (2008) Curr Opin Cell Biol, 20, pp. 235-241; 
Lopez-Otin, C., Matrisian, L.M., Emerging roles of proteases in tumour suppression (2007) Nature Reviews Cancer, 7 (10), pp. 800-808. , DOI 10.1038/nrc2228, PII NRC2228; 
Martin, M.D., Matrisian, L.M., The other side of MMPs: Protective roles in tumor progression (2007) Cancer and Metastasis Reviews, 26 (3-4), pp. 717-724. , DOI 10.1007/s10555-007-9089-4, Forty Years of Metastasis Research: A Tribute to Dr. Isaiah J. Fidler; 
Sahai, E., Mechanisms of cancer cell invasion (2005) Current Opinion in Genetics and Development, 15 (1), pp. 87-96. , DOI 10.1016/j.gde.2004.12.002, PII S0959437X04001881, Oncogenes and Cell Proliferation; 
Wolf, K., Mazo, I., Leung, H., Engelke, K., Von Andrian, U.H., Deryugina, E.I., Strongin, A.Y., Friedl, P., Compensation mechanism in tumor cell migration: Mesenchymal-amoeboid transition after blocking of pericellular proteolysis (2003) Journal of Cell Biology, 160 (2), pp. 267-277. , DOI 10.1083/jcb.200209006; 
Wolf, K., Friedl, P., Molecular mechanisms of cancer cell invasion and plasticity (2006) Br J Dermatol, 154 (SUPPL. 1), pp. 11-15; 
Yilmaz, M., Christofori, G., Lehembre, F., Distinct mechanisms of tumor invasion and metastasis (2007) Trends in Molecular Medicine, 13 (12), pp. 535-541. , DOI 10.1016/j.molmed.2007.10.004, PII S1471491407001888; 
Wicki, A., Christofori, G., The potential role of podoplanin in tumour invasion (2007) British Journal of Cancer, 96 (1), pp. 1-5. , DOI 10.1038/sj.bjc.6603518, PII 6603518; 
Yamaguchi, H., Condeelis, J., Regulation of the actin cytoskeleton in cancer cell migration and invasion (2007) Biochimica et Biophysica Acta - Molecular Cell Research, 1773 (5), pp. 642-652. , DOI 10.1016/j.bbamcr.2006.07.001, PII S0167488906001558; 
Sahai, E., Garcia-Medina, R., Pouyssegur, J., Vial, E., Smurf1 regulates tumor cell plasticity and motility through degradation of RhoA leading to localized inhibition of contractility (2007) Journal of Cell Biology, 176 (1), pp. 35-42. , http://www.jcb.org/cgi/reprint/176/1/35, DOI 10.1083/jcb.200605135; 
Gentile, A., Trusolino, L., Comoglio, P.M., The Met tyrosine kinase receptor in development and cancer (2008) Cancer Metastasis Rev, 27, pp. 85-94; 
Lengyel, E., Sawada, K., Salgia, R., Tyrosine kinase mutations in human cancer (2007) Current Molecular Medicine, 7 (1), pp. 77-84. , http://www.ingentaconnect.com/content/ben/cmm/2007/00000007/00000001/ art00007, DOI 10.2174/156652407779940486; 
Funasaka, T., Raz, A., The role of autocrine motility factor in tumor and tumor microenvironment (2007) Cancer and Metastasis Reviews, 26 (3-4), pp. 725-735. , DOI 10.1007/s10555-007-9086-7, Forty Years of Metastasis Research: A Tribute to Dr. Isaiah J. Fidler; 
Hehlgans, S., Haase, M., Cordes, N., Signalling via integrins: Implications for cell survival and anticancer strategies (2007) Biochimica et Biophysica Acta - Reviews on Cancer, 1775 (1), pp. 163-180. , DOI 10.1016/j.bbcan.2006.09.001, PII S0304419X06000588; 
Douma, S., Van Laar, T., Zevenhoven, J., Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB (2004) Nature, 430, pp. 1034-1039; 
Geiger, T.R., Peeper, D.S., The neurotrophic receptor TrkB in anoikis resistance and metastasis: A perspective (2005) Cancer Research, 65 (16), pp. 7033-7036. , DOI 10.1158/0008-5472.CAN-05-0709; 
Sandri, M.T., Circulating tumor cells (2007) IEO-Scientific Report 2007-ongoing Research 2008, pp. 192-194; 
Gnerlich, J., Jeffe, D.B., Deshpande, A.D., Beers, C., Zander, C., Margenthaler, J.A., Surgical removal of the primary tumor increases overall survival in patients with metastatic breast cancer: Analysis of the 1988-2003 SEER data (2007) Annals of Surgical Oncology, 14 (8), pp. 2187-2194. , DOI 10.1245/s10434-007-9438-0; 
Salama, J.K., Chmura, S.J., Mehta, N., An initial report of a radiation dose-escalation trial in patients with one to five sites of metastatic disease (2008) Clin Cancer Res, 14, pp. 5255-5259; 
Dolle, L., Depypere, H.T., Bracke, M.E., Anti-invasive and anti-metastasis strategies: New roads, new tools and new hopes (2006) Current Cancer Drug Targets, 6 (8), pp. 729-751. , http://docstore.ingenta.com/cgi-bin/ds_deliver/1/u/d/ISIS/33724480.1/ben/ ccdt/2006/00000006/00000008/art00007/9C146EDF9740EFD9116485319359E89FF5E8D1E22C. pdf?link=http://www.ingentaconnect.com/error/delivery\&format=pdf, DOI 10.2174/156800906779010263; 
Fox, S.B., Generali, D.G., Harris, A.L., Breast tumour angiogenesis (2007) Breast Cancer Res, 9, p. 216; 
Shaked, Y., Kerbel, R.S., Antiangiogenic strategies on defense: On the possibility of blocking rebounds by the tumor vasculature after chemotherapy (2007) Cancer Research, 67 (15), pp. 7055-7058. , http://cancerres.aacrjournals.org/cgi/reprint/67/15/7055, DOI 10.1158/0008-5472.CAN-07-0905; 
Formenti, S.C., Demaria, S., Local control by radiotherapy: Is that all there is? (2008) Breast Cancer Res, 10, p. 215; 
Husemann, Y., Geigl, J.B., Schubert, F., Musiani, P., Meyer, M., Burghart, E., Forni, G., Klein, C.A., Systemic Spread Is an Early Step in Breast Cancer (2008) Cancer Cell, 13 (1), pp. 58-68. , DOI 10.1016/j.ccr.2007.12.003, PII S1535610807003728; 
Denys, H., Derycke, L., Hendrix, A., Differential impact of TGF-beta and EGF on fibroblast differentiation and invasion reciprocally promotes colon cancer cell invasion (2008) Cancer Lett, 266, pp. 263-274; 
Milas, L., Mason, K., Liao, Z., Nieder, C., Milas, L., Ang, K.K., Role of cyclooxygenase-2 (COX-2) and its inhibition in tumor biology and radiotherapy (2003) Modification of Radiation Response, pp. 241-258. , Springer Berlin; 
Opdenakker, G., Van Damme, J., The countercurrent principle in invasion and metastasis of cancer cells. Recent insights on the roles of chemokines (2004) International Journal of Developmental Biology, 48 (5-6), pp. 519-527. , DOI 10.1387/ijdb.041796go, Invasion in Cancer and Embryonic Development; 
Nugent, J.L., Bunn Jr., P.A., Matthews, M.J., CNS metastases in small cell bronchogenic carcinoma. Increasing frequency and changing pattern with lengthening survival (1979) Cancer, 44 (5), pp. 1885-1893; 
Camphausen, K., Moses, M.A., Beecken, W.-D., Khan, M.K., Folkman, J., O{\textquoteright}Reilly, M.S., Radiation therapy to a primary tumor accelerates metastatic growth in mice (2001) Cancer Research, 61 (5), pp. 2207-2211; 
Ramaswamy, S., Ross, K.N., Lander, E.S., Golub, T.R., A molecular signature of metastasis in primary solid tumors (2003) Nature Genetics, 33 (1), pp. 49-54. , DOI 10.1038/ng1060; 
Chen, Y.-J., Lin, S.-C., Kao, T., Chang, C.-S., Hong, P.-S., Shieh, T.-M., Chang, K.-W., Genome-wide profiling of oral squamous cell carcinoma (2004) Journal of Pathology, 204 (3), pp. 326-332. , DOI 10.1002/path.1640; 
Perou, C.M., Sorile, T., Eisen, M.B., Van De Rijn, M., Jeffrey, S.S., Ress, C.A., Pollack, J.R., Botstein, D., Molecular portraits of human breast tumours (2000) Nature, 406 (6797), pp. 747-752. , DOI 10.1038/35021093; 
LaTulippe, E., Satagopan, J., Smith, A., Scher, H., Scardino, P., Reuter, V., Gerald, W.L., Comprehensive gene expression analysis of prostate cancer reveals distinct transcriptional programs associated with metastatic disease (2002) Cancer Research, 62 (15), pp. 4499-4506; 
Lapointe, J., Li, C., Giacomini, C.P., Salari, K., Huang, S., Wang, P., Ferrari, M., Pollack, J.R., Genomic profiling reveals alternative genetic pathways of prostate tumorigenesis (2007) Cancer Research, 67 (18), pp. 8504-8510. , http://cancerres.aacrjournals.org/cgi/reprint/67/18/8504, DOI 10.1158/0008-5472.CAN-07-0673; 
Lips, E.H., Van Eijk, R., De Graaf, E.J., Integrating chromosomal aberrations and gene expression profiles to dissect rectal tumorigenesis (2008) BMC Cancer, 8, p. 314; 
Cromer, A., Carles, A., Millon, R., Ganguli, G., Chalmel, F., Lemaire, F., Young, J., Wasylyk, B., Identification of genes associated with tumorigenesis and metastatic potential of hypopharyngeal cancer by microarray analysis (2004) Oncogene, 23 (14), pp. 2484-2498. , DOI 10.1038/sj.onc.1207345; 
Couvelard, A., Hu, J., Steers, G., O{\textquoteright}Toole, D., Sauvanet, A., Belghiti, J., Bedossa, P., Pezzella, F., Identification of Potential Therapeutic Targets by Gene-Expression Profiling in Pancreatic Endocrine Tumors (2006) Gastroenterology, 131 (5), pp. 1597-1610. , DOI 10.1053/j.gastro.2006.09.007, PII S0016508506020014; 
Bertucci, F., Finetti, P., Rougemont, J., Charafe-Jauffret, E., Nasser, V., Loriod, B., Camerlo, J., Viens, P., Gene expression profiling for molecular characterization of inflammatory breast cancer and prediction of response to chemotherapy (2004) Cancer Research, 64 (23), pp. 8558-8565. , DOI 10.1158/0008-5472.CAN-04-2696
},
	month = {2009///},
	pages = {599 - 622},
	abstract = {Malignant tumors invade and metastasize. They consist of cancer cells, evolving through genetic and epigenetic modulation, mixed with tumor-associated host cells, emerging from resident or bone marrow-derived precursors. These cells establish ecosystems to activate cellular programs for local invasion and distant metastasis. Characteristic of such malignancy-related activities is communication inside ecosystems between cells, ligands, receptor protein complexes, and signaling pathways as well as between ecosystems comprising the primary tumor, lymph node and distant metastasis, bone marrow and blood and lymph circulation. Complexity is another characteristic, resulting from: heterogeneity of the cell populations; the numbers of promoter and suppressor genes, their levels of regulation, and the pleiotropic activities of their products; biological redundancy of the molecular mechanisms underpinning invasion-related activities. Clinical attention is paid to putative new targets, namely host cells, individual molecules and their signaling pathways, as well as the effects of current treatment on invasion and metastasis. {\textcopyright} 2009 Springer-Verlag.},
	keywords = {bone marrow, cancer cell, cancer invasion, carcinogenesis, cell activity, Circulation, ecosystem, epigenetics, extracellular matrix, gene control, host cell, human, Humans, lymph node metastasis, metastasis, Metastatic site, microarray analysis, Molecular biology, molecular imaging, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms, nonhuman, pleiotropy, primary tumor, priority journal, Promoter and suppressor genes, promoter region, review, signal transduction, suppressor gene, Therapy guidance, Tumor-associated host cells},
	isbn = {09456317 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67649116432\&partnerID=40\&md5=4c02a29f53858424b4c3191dcd86256c},
	author = {Mareel, M. and Oliveira, M.J. and Madani, I.}
}
@article {2306,
	title = {Cationic liposome-DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cells},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.02.019PubMed ID: 19332382Language of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; CEBIMED, Faculdade de Ci{\^e}ncias da Sa{\'u}, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal; email: mpferraz@ufp.ptChemicals/CAS: 1,2 dioleoyl 3 trimethylammoniopropane, 144189-73-1; DNA, 9007-49-2; transferrin, 82030-93-1; DNA, 9007-49-2; LiposomesReferences: Luo, J., Sun, M.H., Kang, Q., Peng, Y., Jiang, W., Luu, H.H., Gene therapy for bone regeneration (2005) Curr Gene Ther, 5 (2), pp. 167-179;Oreffo, R.O.C., Triffitt, J.T., Future potentials for using osteogenic stem cells and biomaterials in orthopedics (1999) Bone, 25 (2 SUPPL. 1), pp. 5S-9S; 
Laurencin, C.T., Attawia, M.A., Lu, L.Q., Borden, M.D., Lu, H.H., Gorum, W.J., Poly(lactide-co-glycolide)/hydroxyapatite delivery of BMP-2-producing cells: a regional gene therapy approach to bone regeneration (2001) Biomaterials, 22 (11), pp. 1271-1277; 
Katz, J.L., (1996) Applications of materials in medicine and dentistry: orthopedic applications, , Academic Press, San Diego, CA p. 335-46; 
Bucholz, R., Nonallograft osteoconductive bone grafts substitutes (2002) Clin Orthop, 395, pp. 44-52; 
Kirker-Head, C.A., Potential applications and delivery strategies of bone morphogenetic proteins (2000) Adv Drug Deliv Rev, 43, pp. 65-92; 
Chen, Y., Luk, K.D.K., Cheung, K.M.C., Xu, R., Lin, M.C., Lu, W.W., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors (2003) Gene Ther, 10 (16), pp. 1345-1353; 
Tseng, W.-C., Huang, L., Liposome-based gene therapy (1998) Res Focus, 1 (5); 
Leach, J.K., Mooney, D., Bone engineering by controlled delivery of osteoinductive molecules and cells (2004) Expert Opin, 4 (7); 
Dragoo, J.L., Choi, J.Y., Lieberman, J.R., Huang, J., Zuk, P.A., Zhang, J., Bone induction by BMP-2 transduced stem cells derived from human fat (2003) J Orthop Res, 21 (4), pp. 622-629; 
Eliyahu, H., Barenholz, Y., Domb, A.J., Polymers for DNA delivery (2005) Molecules, 10, pp. 34-64; 
Penque, D., Terapia g{\'e}nica: um objectivo ou uma realidade (2000) Boletim Biotecnol, 66, pp. 16-19; 
Winn, S.R., Hu, Y., Sfeir, C., Hollinger, J.O., Gene therapy approaches for modulating bone regeneration (2000) Adv Drug Deliv Rev, 42 (1-2), pp. 121-138; 
Lima, N., Mota, M., (2003) Biotecnologia, fundamentos e aplica{\c c}{\~o}es, , Lidel, Lisboa p. 368-73; 
Luo, D., Saltzman, W.M., Synthetic DNA delivery systems (2000) Nat Biotechnol, 18, pp. 33-37; 
Kikuchi, H., Suzuki, N., Ebihara, K., Morita, H., Ishii, Y., Kikuchi, A., Gene delivery using liposome technology (1999) J Control Release, 62 (1-2), pp. 269-277; 
Liu, F., Huang, L., Development of non-viral vectors for systemic gene delivery (2002) J Control Release, 78 (1-3), pp. 259-266; 
Girao da Cruz, M.T., Simoes, S., Pires, P.P.C., Nir, S., Pedroso de Lima, M.C., Kinetic analysis of the initial steps involved in lipoplex-cell interactions: effect of various factors that influence transfection activity (2001) Biochim Biophys Acta (BBA) - Biomembr, 1510 (1-2), pp. 136-151; 
Zuidam, N.J., Barenholz, Y., Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery (1998) Biochim Biophys Acta (BBA) - Biomembr, 1368 (1), pp. 115-128; 
Tros de Ilarduya, C., Arangoa, M.A., Moreno-Aliaga, M.J., Duzgunes, N., Enhanced gene delivery in vitro and in vivo by improved transferrin-lipoplexes (2002) Biochim Biophys Acta (BBA) - Biomembr, 1561 (2), pp. 209-221; 
Simoes, S., Pires, P., Cruz, M.T.G.d., Duzgunes, N., Pedroso de Lima, M.C., Gene delivery by cationic liposome-DNA complexes containing transferrin or serum albumin (2003) Methods Enzymol, 373, pp. 369-383; 
Woo-Jeong, C., Jin-Ki, K., Sung-Hee, C., Jeong-Sook, P., Woong Shick, A., Kima, C.-K., Low toxicity of cationic lipid-based emulsion for gene transfer (2004) Biomaterials, 25 (27), pp. 5893-5903; 
Koe, G.S., Way, H.L., Quentingco, G.M., Smith, J.G., Wedeking, T., Niven, R.W., The effect of mixing on the formation of DNA/liposome complexes (1997) Pharmacy, p. 14; 
Lasic, D.D., Templeton, N.S., Liposomes in gene therapy (1996) Adv Drug Deliv Rev, 20 (2-3), pp. 221-266; 
Pedroso de Lima, M.C., Simoes, S., Pires, P., Faneca, H., Duzgunes, N., Cationic lipid-DNA complexes in gene delivery: from biophysics to biological applications (2001) Adv Drug Deliv Rev, 47 (2-3), pp. 277-294; 
Hirsch-Lerner, D., Zhang, M., Eliyahu, H., Ferrari, M.E., Wheeler, C.J., Barenholz, Y., Effect of "helper lipid" on lipoplex electrostatics (2005) Biochim Biophys Acta (BBA) - Biomembr, 1714 (2), pp. 71-84; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57 (1), pp. 57-62; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles (2003) Biomaterials, 24 (7), pp. 1255-1264; 
Mislick, K.A., Baldeschwieler, J.D., Evidence for role of proteoglycans in cation-mediated gene transfer (1996) Proc Natl Acad Sci USA, 93 (22), pp. 12349-12354; 
Duzgunes, N., Nir, S., Mechanisms and kinetics of liposome-cell interactions (1999) Adv Drug Deliv Rev, 40 (1-2), pp. 3-18; 
Radwan Almofti, M., Harashima, H., Shinohara, Y., Almofti, A., Baba, Y., Kiwada, H., Cationic liposome-mediated gene delivery: biophysical study and mechanism of internalization (2003) Arch Biochem Biophys, 410 (2), pp. 246-253; 
Sim{\~o}es, S., Filipe, A., Faneca, H., Mano, M., Penacho, N., Duzgunes, N., Cationic liposomes for gene delivery (2005) Expert Opin, 2 (2); 
Rejman, J., Bragonzi, A., Conese, M., Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes (2005) Mol Ther, 12 (3), pp. 468-474
},
	month = {2009///},
	pages = {2142 - 2151},
	abstract = {Modulation of the biological pathways responsible for fracture repair and osteogenisis may accelerate regeneration. Gene therapy is an alternative method for the release of osteogenisis-stimulating proteins into tissues. The development of vectors for gene release is still a problem in terms of ethics and techniques. In this work we evaluated whether cationic liposomes constitute a valuable strategy for the release of genetic material into bone tissue cells as non-viral vectors. Liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium propane (DOTAP)-2-dioleoyl-sn-glycero-3-phosphatidylethanolamine and DOTAP-cholesterol, and characterized according to their size, zeta potential, DNA protection capacity and cytotoxicity. Transfection studies were also carried out using pCMVβ-gal plasmid in two osteoblastic cell lines (MG63 and MC3T3-E1) and in the 294T line, varying the charge ratio and the applied DNA dose. Inclusion of transferrin to increase the expression was also tested. The results suggest that there is great dependency between the transfection activity and the lipid formulation, the charge ratios of the complexes, the applied DNA dose and the cell type. There were even some differences concerning both osteoblastic lines under study. The cells of the MC3T3-E1 line present greater expression levels than the cells of the MG-63 line. The conjugation of the transferrin with the complexes contributes to the increase in transfection levels, possibly due to an increase in internalization of complexes. It is thus a good strategy for inducing the expression of specific genes in osteoblast-like cells. {\textcopyright} 2009 Acta Materialia Inc.},
	keywords = {1,2 dioleoyl 3 trimethylammoniopropane, animal cell, Animals, article, bone cell, bone development, bone tissue, Cationic liposomes, Cell Line, cell type, complex formation, controlled study, Cytotoxicity, DNA, drug mechanism, gene expression regulation, Gene therapy, Gene transfer, genetic transfection, Genetic Vectors, human, human cell, internalization, lipoplex, liposome, Liposomes, materials testing, Mice, nonhuman, nonviral gene delivery system, osteoblast, Osteoblasts, Osteogenesis, Particle Size, plasmid, priority journal, Transfection, transferrin, Zeta potential},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67349188484\&partnerID=40\&md5=b675ab7e6e97523c5d8923fa6e7f0d8b},
	author = {Oliveira, A.C. and Ferraz, M.P. and Monteiro, F.J. and Sim{\~o}es, S.}
}
@book {2302,
	title = {Cell division detection on the arabidopsis thaliana root},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5524 LNCS},
	year = {2009},
	note = {Conference code: 77027Cited By (since 1996): 5Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02172-5_23Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, p. 13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. on Circuits and Systems I-Regul. Papers, 53 (11), pp. 2405-2414; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3d fluorescence microscopy image sequences (2008) Workshop on Bio-Image Informatics: Biological Imaging, Computer Vision and Data Mining; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proceedings of the Int. Conference on Image Analysis and Processing, pp. 757-761; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A.C.: Detection of lung nodule candidates in chest radiographs. In: Mart{\'\i}, J., Bened{\'\i}, J.M., Mendon{\c c}a, A.M., Serrat, J. (eds.) IbPRIA 2007. LNCS, 4478, pp. 170-177. Springer, Heidelberg (2007)Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., A hybrid approach for cell image segmentation (2008) LNCS, 5112, pp. 739-749. , Campilho, A, Kamel, M.S, eds, ICIAR 2008, Springer, Heidelberg
Sponsors: International Association for Pattern Recognition (IAPR); Fundacao para a Ciencia e a Tecnologia (FCT)},
	month = {2009///},
	pages = {168 - 175},
	address = {Povoa de Varzim},
	abstract = {The study of individual plant cells and their growth structure is an important focus of research in plant genetics. To obtain development information at cellular level, researchers need to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Within this research field it is important to understand mechanisms like cell division and elongation of developing cells. We describe a tool to automatically search for cell division in the Arabidopsis thaliana using information of nuclei shape. The nuclei detection is based on a convergence index filter. Cell division detection is performed by an automatic classifier, trained through cross-validation. The results are further improved by a stability criterion based on the Mahalanobis distance of the shape of the nuclei through time. With this approach, we can achieve a correct detection rate of 94.7\%. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {A-stability, Arabidopsis thaliana, Automatic classifiers, Bioinformatics, Cell divisions, Cell membranes, cell proliferation, Cellular levels, confocal microscopy, Cross validation, Detection rates, Growth structures, Image analysis, In-plants, In-Vivo imaging, Mahalanobis distances, Pattern recognition, Plant cells, research, Research fields, Stability criteria},
	isbn = {03029743 (ISSN); 3642021719 (ISBN); 9783642021718 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68749115481\&partnerID=40\&md5=2c491950d3b00a9dd873aeaefdf5edcb},
	author = {Marcuzzo, M. and Guichard, T. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2319,
	title = {Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {396-398},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAEdoi: 10.4028/0-87849-353-0.303Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do PortoPortugal; email: alejopv@ineb.up.ptReferences: Warashina, H., Sakano, S., Kitamura, S., Yamauchi, K.I., (2003) Biomaterials, 24, pp. 3655-3661;Scarano, A., Piattelli, M., Caputi, S., Favero, G.A., Piattelli, A., (2004) J Periodontol, 75, pp. 292-296; 
Manicone, P.F., Rossi Iommetti, P., Raffaelli, L., (2007) J Dent, 35, pp. 819-826; 
Covacci, V., Bruzzese, N., Maccauro, G., Andreassi, C., (1999) Biomaterials, 20, pp. 371-376; 
Silva, V.V., Lameiras, F.S., Lobato, Z.I., (2002) J Biomed Mater Res, 63, pp. 583-590; 
Oliva, J., Oliva, X., Oliva, J., (2007) Int J Oral Maxillofac Implants, 22, pp. 430-435; 
Klinge, B., Hultin, M., Berglundh, T., (2005) Dent Clin North Am, 49, pp. 661-676; 
Wennerberg, A., Albrektsson, T., Andersson, B., Krol, J.J., (1995) Clin Oral Impl Res, 6, pp. 24-30; 
Brunette, D.M., Kenner, G.S., Gould, T.R., (1983) J Dent Res, 62, pp. 1045-1048; 
Pelaez, A., Pareja, A., Garcia, C., Marquez, M., (2005) Key Eng Mater, 284, pp. 593-596; 
Curtis, A., Wilkinson, C., (1997) Biomaterials, 18, pp. 1573-1583; 
Ito, Y., (1999) Biomaterials, 20, pp. 2333-2342; 
Tan, J., Saltzman, W.M., (2004) Biomaterials, 25, pp. 3593-3601; 
Gallego, D., Ferrell, N., Sun, Y., Hansford, D.J., (2008) Mater Sci Eng C, 28, pp. 353-358; 
Xia, Y., Whitesides, G.M., (1998) Angew. Chem. Int. Ed, 37, pp. 550-575
},
	month = {2009///},
	pages = {303 - 306},
	abstract = {Cell behaviour such as adhesion, morphology, proliferation and functional activity are highly influenced by surface properties including hydrophobicity, roughness, texture and morphology. These surface properties may be controlled using a mixture of additive coating techniques to produce glass coatings by sol-gel process and soft lithography on dental ceramics. The purpose of this work was to compare cell adhesion and early orientation of Human Bone Marrow (HBM) cells cultured on micro-patterned (micro-PGC) and on flat glass coatings (FGC) produced by sol-gel processing. Spin coating was used to apply SiO                        2 flat coatings on glass substrates as model surfaces. Photolithography was applied to produce master patterns with microscale dimensions. A moulding technique was used to print micropatterned SiO 
                        2 glass coatings produced by a sol-gel process. The coatings were then sintered, sterilized and cultured with HBM cells derived from primary cultures, using a standardized protocol, for 1 and 7 days. Cell morphology and orientation were observed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Flat and MPGC with line shaped features were produced. Cells presented a typical osteoblastic morphology on flat surfaces while slimmer, preferentially oriented and more elongated morphologies could be seen on line micro-patterned surfaces. HBM cells cultured on flat glass coatings showed increased tendency to spread and to assume more randomized proliferation when compared to the cells on the micro-patterned glass coatings. Micro-patterned glass coatings showed higher orientation control and smaller delay in the rate of proliferation, in early stages of in vitro culture as compared to flat coatings. These preliminary studies revealed that Micro-PGC induce significant morphological changes and controlled orientation of HBM cells during early stages of cell proliferation.
},
	keywords = {Adhesion, cell adhesion, Cell behaviours, Cell culture, Cell morphologies, cell proliferation, Cells, Coatings, Colloids, Confocal laser scanning microscopies, confocal microscopy, Contact guidance, Controlled orientations, cytology, Dental ceramics, Dental Materials, Flat glass, Flat surfaces, Functional activities, Gelation, Gels, glass, Glass ceramics, Glass coatings, Glass substrates, In-vitro, Micro-patterned, Micro-patterned surfaces, Micro-scale, Micromachining, Micropatterning, Model surfaces, Morphological changes, morphology, Nanostructured materials, Orientation controls, Primary cultures, Scanning, Scanning electron microscopy, Scanning electrons, SEM, Silicon compounds, Soft lithographies, Sol-gel, Sol-gel process, Sol-gel processing, Sol-gels, Sols, Surface Properties, Thermal spraying, Trace analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849141765\&partnerID=40\&md5=7a4c975e741442308a8db69108047673},
	author = {Pelaez-Vargas, A. and Ferrell, N. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
@book {2301,
	title = {Chromatographic pattern recognition using optimized one-class classifiers},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5524 LNCS},
	year = {2009},
	note = {Conference code: 77027Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02172-5_58Language of Original Document: EnglishCorrespondence Address: Sousa, A. V.; Instituto de Engenharia Biom{\'e}dica, Rua Roberto Frias, Porto 4200-465, Portugal; email: ats@isep.ipp.ptReferences: Skurichina, M., Raudys, S., Duin, R.P.W., K-Nearest Neighbors Directed Noise Injection in Multilayer Perceptron Training (2000) IEEE Transactions on Neural Networks, 11;Chawla, N.V., Japkowicz, N., Kolcz, A., Editorial: Special Issue: Learning from Imbal-anced Data Sets (2004) Sigkdd Explorations, 6, pp. 1-6; 
Tax, D.M.J., (2001) One-class Classification; Concept-learning in the Absence of Counterexamples, , Ph.D. thesis: Delft University of Technology; 
Durand, G., Seta, N., Protein Glycosylation and Diseases: Blood and Urinary Oligosac-charides as Markers for Diagnosis and Therapeutic Monitoring (2000) Clin. Chem, 46, pp. 795-805; 
Sewell, A.C., Urinary Oligosaccharides. Techniques in Diagnostic Human (1991) Biochemical Genetics, pp. 219-231; 
Pekalska, E., Duin, R.P.W., Dissimilarity Representations Allow for Building Good Classifiers (2002) Pattern Recogn. Lett, 23, pp. 943-956; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Dissimilarity-based Classification of Chromatographic Profiles (2008) Pattern Analysis and Applications, 11 (3-4), pp. 409-423; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Minimizing the Imbalance Problem in Chromatographic Profile Classification with One-class Classifiers (2008) LNCS, 5112, pp. 413-422. , Campilho, A, Kamel, M.S, eds, ICIAR 2008, Springer, Heidelberg; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Chromatographic Pattern Classification (2008) IEEE Trans. on Biomedical Engineering, 55 (6), pp. 1687-1696; 
Visa, S., Ralescu, A., Learning Imbalanced and Overlapping Classes Using Fuzzy Sets (2003) Workshop on Learning from Imbalanced Datasets II, ICML, , Washington DC
Sponsors: International Association for Pattern Recognition (IAPR); Fundacao para a Ciencia e a Tecnologia (FCT)},
	month = {2009///},
	pages = {449 - 456},
	address = {Povoa de Varzim},
	abstract = {This paper presents a method for automating the selection of the rejection rate of one-class classifiers aiming at optimizing the classifier performance. These classifiers are used in a new classification approach to deal with class imbalance in Thin-Layer Chromatography (TLC) patterns, which is due to the huge difference between the number of normal and pathological cases, as a consequence of the rarity of Lysosomal Storage Disorders (LSD) diseases. The classification is performed in two decision stages, both implemented using optimized one-class classifiers: the first stage aims at recognizing most of the normal samples; the outliers of this first decision level are presented to the second stage, which is a multiclassifier prepared to deal with both pathological and normal patterns. The results that were obtained proved that the proposed methodology is able to overcome some of the difficulties associated with the choice of the rejection rate of one-class classifiers, and generally contribute to the minimization of the imbalance problem in TLC pattern classification. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {Chromatographic analysis, Class imbalance, Classification approach, Classifier performance, Classifiers, Decision levels, Image analysis, Imbalance problem, Learning systems, Lysosomal storage disorder, Multi-classifier, One-class classifier, Optimization, Pathological case, Pattern classification, Pattern recognition, Rejection rates, Thin layer chromatography},
	isbn = {03029743 (ISSN); 3642021719 (ISBN); 9783642021718 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68749107350\&partnerID=40\&md5=17cc4075de5306819dcd1bfc005171d6},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2312,
	title = {Contextual classification of image patches with latent aspect models},
	journal = {Eurasip Journal on Image and Video ProcessingEurasip J. Image Video Process.},
	volume = {2009},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusArt. No.: 602920doi: 10.1155/2009/602920Language of Original Document: EnglishCorrespondence Address: Monay, F.; Idiap Research Institute Martigny, 1920 Martigny, Switzerland; email: florent.monay@idiap.chReferences: Kumar, S., Hebert, M., Discriminative random fields: A discriminative framework for contextual interaction in classification, 2, pp. 1150-1157. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France;Lazebnik, S., Schmid, C., Ponce, J., Affine-invariant local descriptors and neighborhood statistics for texture recognition, 1, pp. 649-655. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Vogel, J., Schiele, B., Natural scene retrieval based on a semantic modeling step, 3115, pp. 207-215. , Proceedings of the 3rd International Conference on Image and Video Retrieval (CIVR 04) July 2004 Dublin, Ireland Lecture Notes in Computer Science; 
Shotton, J., Winn, J., Rother, C., Criminisi, A., TextonBoost: Joint appearance, shape and context modeling for multi-class object recognition and segmentation, 3951, pp. 1-15. , Proceedings of the 9th European Conference on Computer Vision (ECCV 06) May 2006 Graz, Austria Lecture Notes in Computer Science; 
Lowe, D.G., Distinctive image features from scale-invariant keypoints (2004) International Journal of Computer Vision, 60 (2), pp. 91-110; 
Dorko, G., Schmid, C., Selection of scale-invariant parts for object class recognition, 1, pp. 634-640. , Proceedings of the 9th IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Fei-Fei, L., Perona, P., A Bayesian hierarchical model for learning natural scene categories, 2, pp. 524-531. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 05) June 2005 San Diego, Calif, USA; 
Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., Van Gool, L., Modeling scenes with local descriptors and latent aspects, 1, pp. 883-890. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Sivic, J., Russell, B.C., Efros, A.A., Zisserman, A., Freeman, W.T., Discovering object categories in image collections, , Proceedings of the 10th IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Li, S.Z., (1995) Markov Random Field Modeling in Computer Vision, , New York, NY, USA Springer; 
Kumar, S., Hebert, M., Man-made structure detection in natural images using a causal multiscale random field, 1, pp. 119-126. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 03) June 2003 Madison, Wis, USA; 
Murphy, K., Torralba, A., Freeman, W., Using the forest to see the trees: A graphical model relating features, objects and scenes, , Proceedings of the Neural Information Processing Systems December 2003 Vancouver, Canada; 
Geman, S., Geman, D., Stochastic relaxation, Gibbs distributions, and the Bayesian restoration of images (1984) IEEE Transactions on Pattern Analysis and Machine Intelligence, 6 (6), pp. 721-741; 
Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., A thousand words in a scene (2007) IEEE Transactions on Pattern Analysis and Machine Intelligence, 29 (9), pp. 1575-1589; 
Quelhas, P., Odobez, J.-M., Multi-level local descriptor quantization for bag-of-visterms image representation, pp. 242-249. , Proceedings of the 6th ACM International Conference on Image and Video Retrieval (CIVR 07) July 2007 Amsterdam, The Netherlands; 
Csurka, G., Dance, C., Fan, L., Willamowski, J., Bray, C., Visual categorization with bags of keypoints, pp. 59-74. , Proceedings of the European Conference on Computer Vision (ECCV 04) May 2004 Prague, Czech Republic; 
Marszaek, M., Schmid, C., Spatial weighting for bag-of-features, 2, pp. 2118-2125. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Wang, G., Zhang, Y., Fei-Fei, L., Using dependent regions for object categorization in a generative framework, 2, pp. 1597-1604. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Sivic, J., Zisserman, A., Video Google: A text retrieval approach to object matching in videos, 2, pp. 1470-1477. , Proceedings of the 9th IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Willamowski, J., Arregui, D., Csurka, G., Dance, C.R., Fan, L., Categorizing nine visual classes using local appearance descriptors, , Proceedings of the Workshop on Learning for Adaptable Visual Systems (LAVS), in Conjunction with the International Conference on Pattern Recognition (ICPR 04) August 2004 Cambridge, UK; 
Bosch, A., Zisserman, A., Muoz, X., Scene classification via pLSA, 3954, pp. 517-530. , Proceedings of the 9th European Conference on Computer Vision (ECCV 06) May 2006 Graz, Austria Lecture Notes in Computer Science; 
Hofmann, T., Unsupervised learning by probabilistic latent semantic analysis (2001) Machine Learning, 42 (12), pp. 177-196; 
Blei, D.M., Ng, A.Y., Jordan, M.I., Latent Dirichlet allocation (2003) Journal of Machine Learning Research, 3 (45), pp. 993-1022; 
Pal, N.R., Pal, S.K., A review on image segmentation techniques (1993) Pattern Recognition, 26 (9), pp. 1277-1294; 
Carson, C., Thomas, M., Belongie, S., Hellerstein, J.M., Malik, J., Blob-world: A system for region-based image indexing and retrieval, pp. 509-516. , Proceedings of the 3rd International Conference on Visual Information Systems (VISUAL 99) June 1999 Amsterdam, The Netherlands Springer; 
Duygulu, P., Barnard, K., De Freitas, J.F.G., Forsyth, D.A., Object recognition as machine translation: Learning a lexicon for a fixed image vocabulary, 2353, pp. 97-112. , Proceedings of the 7th European Conference on Computer Vision (ECCV 02) May 2002 Copenhagen, Denmark Lecture Notes in Computer Science; 
Vogel, J., Schiele, B., A semantic typicality measure for natural scene categorization, 3175, pp. 195-203. , Proceedings of the 26th DAGM Pattern Recognition Symposium August-September 2004 Tbingen, Germany Lecture Notes in Computer Science; 
Verbeek, J.J., Triggs, B., Region classification with Markov field aspect models, pp. 1-8. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 07) June 2007 Minneapolis, Minn, USA; 
Shi, J., Malik, J., Normalized cuts and image segmentation, pp. 731-737. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 97) June 1997 San Juan, Puerto Rico, USA; 
Kumar, M.P., Torr, P.H.S., Zisserman, A., Obj cut, 1, pp. 18-25. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 05) June 2005 San Diego, Calif, USA; 
Leibe, B., Leonardis, A., Schiele, B., Combined object categorization and segmentation with an implicit shape model, pp. 17-32. , Proceedings of the 8th European Conference on Computer Vision (ECCV 04) May 2004 Prague, Czech Republic; 
Russell, B.C., Freeman, W.T., Efros, A.A., Sivic, J., Zisserman, A., Using multiple segmentations to discover objects and their extent in image collections, 2, pp. 1605-1612. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Monay, F., Quelhas, P., Odobez, J.-M., Gatica-Perez, D., Integrating co-occurrence and spatial contexts on patch-based scene segmentation, p. 14. , Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshop (CVPR 06) June 2006 New York, NY, USA; 
Cao, L., Fei-Fei, L., Spatially coherent latent topic model for concurrent object segmentation and classification, , Proceedings of the 11th IEEE International Conference on Computer Vision (ICCV 07) October 2007 Rio de Janeiro, Brazil; 
Liu, D., Chen, T., Background cutout with automatic object discovery, 4, pp. 345-348. , Proceedings of IEEE International Conference on Image Processing (ICIP 07) September 2007 San Antonio, Tex, USA; 
Tuytelaars, T., Van Gool, L., Content-based image retrieval based on local affinely invariant regions, 1614, pp. 493-500. , Proceedings of the 3rd International Conference on Visual Information and Information Systems June 1999 Amsterdam, The Netherlands Lecture Notes in Computer Science; 
Mikolajczyk, K., Schmid, C., A performance evaluation of local descriptors, 2, pp. 257-263. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 03) June 2003 Madison, Wis, USA; 
Mikolajczyk, K., Tuytelaars, T., Schmid, C., A comparison of affine region detectors (2005) International Journal of Computer Vision, 65 (12), pp. 43-72; 
Baeza-Yates, R., Ribeiro-Neto, B., (1999) Modern Information Retrieval, , New York, NY, USA ACM Press; 
Monay, F., Quelhas, P., Gatica-Perez, D., Odobez, J.-M., Constructing visual models with a latent space approach, 3940, pp. 115-126. , Proceedings of the Subspace, Latent Structure and Feature Selection, Statistical and Optimization, Perspectives Workshop (SLSFS 05) February 2006 Bohinj, Slovenia Lecture Notes in Computer Science; 
Fergus, R., Fei-Fei, L., Perona, P., Zisserman, A., Learning object categories from Googles image search, 2, pp. 1816-1823. , Proceedings of the 10th IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Buntine, W.L., Variational extensions to em and multinomial PCA, 2430, pp. 23-34. , Proceedings of the 13th European Conference on Machine Learning August 2002 Helsinki, Finland Lecture Notes in Computer Science; 
Bishop, C., (1995) Neural Networks for Pattern Recognition, , Oxford, UK Oxford University
},
	month = {2009///},
	abstract = {We present a novel approach for contextual classification of image patches in complex visual scenes, based on the use of histograms of quantized features and probabilistic aspect models. Our approach uses context in two ways: (1) by using the fact that specific learned aspects correlate with the semantic classes, which resolves some cases of visual polysemy often present in patch-based representations, and (2) by formalizing the notion that scene context is image-specificwhat an individual patch represents depends on what the rest of the patches in the same image are. We demonstrate the validity of our approach on a man-made versus natural patch classification problem. Experiments on an image collection of complex scenes show that the proposed approach improves region discrimination, producing satisfactory results and outperforming two noncontextual methods. Furthermore, we also show that co-occurrence and traditional (Markov random field) spatial contextual information can be conveniently integrated for further improved patch classification.},
	isbn = {16875176 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-61449241990\&partnerID=40\&md5=b909ab8585061d7873ec0fe14a47dba8},
	author = {Monay, F. and Quelhas, P. and Odobez, J.-M. and Gatica-Perez, D.}
}
@article {2315,
	title = {Corrected and improved model for educational simulation of neonatal cardiovascular pathophysiology},
	journal = {Simulation in HealthcareSimul. Healthc.},
	volume = {4},
	year = {2009},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: Scopusdoi: 10.1097/SIH.0b013e31818b27a8Language of Original Document: EnglishCorrespondence Address: Zijlmans, M.; INEB-Instituto de Engenharia Biom{\'e}dica, Porto, PortugalReferences: S{\'a} Couto, C.D., Van Meurs, W.L., Goodwin, J.A., Andriessen, P., A model for educational simulation of neonatal cardiovascular pathophysiology (2006) Simul Healthc, 1, pp. 4-12;Goodwin, J.A., Van Meurs, W.L., S{\'a} Couto, C.D., Beneken, J.E.W., Graves, S.A., A model for educational simulation of infant cardiovascular physiology (2004) Anesth Analg, 99, pp. 1655-1664; 
Beneken, J.E.W., A mathematical approach to cardiovascular function (1965) The Uncontrolled Human System, , Thesis, University of Utrecht, The Netherlands; 
Andriessen, P., Bambang Oetomo, S., Peters, C., Vermeulen, B., Wijn, P.F.F., Blanco, C.E., Baroreceptor reflex sensitivity in human neonates: The effect of postmenstrual age (2005) J Physiol, 568, pp. 333-341; 
Segar, J.L., Roghair, R.D., Segar, E.M., Bailey, M.C., Scholz, T.D., Lamb, F.S., Early gestation dexamethasone alters baroreflex and vascular responses in newborn lambs before hypertension (2006) Am J Physiol Regul Integr Comp Physiol, 291, pp. R481-R488; 
Wallgren, G., Barr, M., Rudhe, U., Hemodynamic studies of induced acute hypo- and hypervolemia in the newborn infant (1964) Acta Paediatr, 53, pp. 1-12
},
	month = {2009///},
	pages = {49 - 53},
	abstract = {We identified errors in the software implementation of the mathematical model presented in: S{\'a} Couto CD, van Meurs WL, Goodwin JA, Andriessen P. A model for educational simulation of neonatal cardiovascular pathophysiology. Simul Healthcare 2006;1:4-12. Simulation results obtained with corrected code are presented for future reference. All but one of the simulation results do not differ by more than 9\% from the previously published results. The heart rate response to acute loss of 30\% of blood volume, simulated with corrected code is stronger than published target data. This modeling error was masked by errors in code implementation. We improved this response and the model by adjusting the gains and adding thresholds and saturations in the baroreflex model. General considerations on identification of model and code errors and model validity are presented. Copyright {\textcopyright} 2009 Society for Simulation in Healthcare.},
	keywords = {aorta coarctation, article, Baroreflex, bleeding, blood pressure, blood volume, Cardiac Output, cardiovascular disease, Cardiovascular Diseases, Cardiovascular physiology, computer program, Computer simulation, Congenital heart defect, congenital heart malformation, diastolic blood pressure, Education, Medical, Erratum, Fallot tetralogy, great vessels transposition, heart output, Heart rate, heart right ventricle pressure, human, Humans, Infant, Newborn, lung pressure, mathematical model, mean arterial pressure, medical education, medical error, methodology, Neonate, Neonatology, newborn, patent ductus arteriosus, pathophysiology, physiology, pressoreceptor reflex, priority journal, simulation, Software Design, systolic blood pressure, Validation, validity, vascular resistance, Verification, vital sign},
	isbn = {15592332 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-62849083921\&partnerID=40\&md5=8cf0edc0b85c1bd093cd9b3a8c70de77},
	author = {Zijlmans, M. and S{\'a}-Couto, C.D. and van Meurs, W.L. and Goodwin, J.A. and Andriessen, P.}
}
@article {2323,
	title = {The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 41Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2008.09.048PubMed ID: 18952279Language of Original Document: EnglishCorrespondence Address: Barrias, C.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, R. Campo Alegre 823, 4150-180 Porto, Portugal; email: ccbarrias@ineb.up.ptChemicals/CAS: fibronectin, 86088-83-7; gold, 7440-57-5; thiol derivative, 13940-21-1; Biocompatible Materials; Fibronectins; VitronectinReferences: Wilson, C.J., Clegg, R.E., Leavesley, D.I., Pearcy, M.J., Mediation of biomaterial-cell interactions by adsorbed proteins: a review (2005) Tissue Eng, 11, pp. 1-18;Garc{\'\i}a, A.J., Vega, M.D., Boettiger, D., Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation (1999) Mol Biol Cell, 10, pp. 785-798; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res A, 66, pp. 247-259; 
Underwood, P.A., Bennett, F.A., A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin (1989) J Cell Sci, 93, pp. 641-649; 
Steele, J.G., Johnson, G., Underwood, P.A., Role of serum vitronectin and fibronectin in adhesion of fibroblasts following seeding onto tissue culture polystyrene (1992) J~Biomed Mater Res, 26, pp. 861-884; 
Steele, J.G., Dalton, B.A., Johnson, G., Underwood, P.A., Polystyrene chemistry affects vitronectin activity: an explanation for cell attachment to tissue culture polystyrene but not to unmodified polystyrene (1993) J Biomed Mater Res, 27, pp. 927-940; 
Underwood, P.A., Steele, J.G., Dalton, B.A., Effects of polystyrene surface chemistry on the biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies (1993) J Cell Sci, 104, pp. 793-803; 
Suzuki, S., Oldberg, A., Hayman, E.G., Pierschbacher, M.D., Ruoslahti, E., Complete amino acid sequence of human vitronectin deduced from cDNA. Similarity of cell attachment sites in vitronectin and fibronectin (1985) EMBO J, 4, pp. 2519-2524; 
Lee, M.H., Ducheyne, P., Lynch, L., Boettiger, D., Composto, R.J., Effect of biomaterial surface properties on fibronectin-alpha5beta1 integrin interaction and cellular attachment (2006) Biomaterials, 27, pp. 1907-1916; 
Grinnell, F., Feld, M.K., Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium (1982) J Biol Chem, 257, pp. 4888-4893; 
Sousa, S.R., Lamghari, M., Sampaio, P., Moradas-Ferreira, P., Barbosa, M.A., Osteoblast adhesion and morphology on TiO 2 depends on the competitive preadsorption of albumin and fibronectin (2008) J Biomed Mater Res A, 84, pp. 281-290; 
Fabrizius-Homan, D.J., Cooper, S.L., Competitive adsorption of vitronectin with albumin, fibrinogen, and fibronectin on polymeric biomaterials (1991) J Biomed Mater Res, 25, pp. 953-971; 
Bale, M.D., Wohlfahrt, L.A., Mosher, D.F., Tomasini, B., Sutton, R.C., Identification of vitronectin as a major plasma protein adsorbed on polymer surfaces of different copolymer composition (1989) Blood, 74, pp. 2698-2706; 
Thomas, C.H., McFarland, C.D., Jenkins, M.L., Rezania, A., Steele, J.G., Healy, K.E., The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry (1997) J Biomed Mater Res, 37, pp. 81-93; 
McFarland, C.D., Thomas, C.H., DeFilippis, C., Steele, J.G., Healy, K.E., Protein adsorption and cell attachment to patterned surfaces (2000) J Biomed Mater Res, 49, pp. 200-210; 
Love, J.C., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1169; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-78; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Faucheux, N., Schweiss, R., L{\"u}tzow, K., Werner, C., Groth, T., Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies (2004) Biomaterials, 25, pp. 2721-2730; 
Bernards, M.T., Jiang, S., pH-induced conformation changes of adsorbed vitronectin maximize its bovine aortic endothelial cell binding ability (2008) J Biomed Mater Res A; 
Martins, M.C., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) J~Biomed Mater Res A, 67, pp. 158-171; 
Rodrigues, S.N., Gon{\c c}alves, I.C., Martins, M.C., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Chamberlain, J., Yamagami, T., Colletti, E., Theise, N.D., Desai, J., Frias, A., Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep (2007) Hepatology, 46, pp. 1935-1945; 
(1993) Iodine-125. A guide to radioiodination techniques, , Amersham Life Science, Little Chalfort; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, pp. 183-214. , Williams D.F. (Ed), CRC Press Inc., Harcover; 
Arima, Y., Iwata, H., Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers (2007) Biomaterials, 28, pp. 3074-3082; 
Capadona, J.R., Collard, D.M., Garcia, A.J., Fibronectin adsorption and cell adhesion to mixed monolayers of tri(ethylene glycol)- and methyl-terminated alkanethiols (2003) Langmuir, 19, pp. 1847-1852; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (2000) J Biomed Mater Res, 49, pp. 435-447; 
Wertz, C.F., Santore, M.M., Fibrinogen adsorption on hydrophilic and hydrophobic surfaces: geometrical and energetic aspects of interfacial relaxations (2002) Langmuir, 18, pp. 706-715; 
Pitt, W.G., Fabrizius-Homan, D.J., Mosher, D.F., Cooper, S.L., Vitronectin adsorption on polystyrene and oxidized polystyrene (1989) J Colloid Interface Sci, 129, pp. 231-239; 
Seiffert, D., Smith, J.W., The cell adhesion domain in plasma vitronectin is cryptic (1997) J Biol Chem, 272, pp. 13705-13710
},
	month = {2009///},
	pages = {307 - 316},
	abstract = {The objective of this study was to compare the biological effects of two key cell-adhesive proteins, fibronectin (FN) and vitronectin (VN), upon adsorption onto molecularly-designed model surfaces. Single-component and mixed self-assembled monolayers (SAMs) of alkanethiols on gold with OH and CH                        3 terminal groups were prepared at 100\%, 65\%, 36\% and 0\% of OH at the surface, to generate a range of surfaces with a simple chemistry and a wettability gradient. FN and VN were adsorbed under non-competitive (single-protein solutions) and competitive (multi-protein solutions) conditions, and compared at different levels: adsorbed amount (radiolabelling), elution, functional presentation of cell-binding domains (ELISA), and role in mediating cell adhesion (antibody-based assay). The observed trends were related to mesenchymal stem cell response in terms of adhesion and overall cell morphology. Under non-competitive conditions, adsorption of both proteins increased with surface hydrophobicity. The presence of competitive proteins significantly decreased the adsorbed amounts, although both proteins were still detected in all SAMs. Adsorption of FN followed a trend similar to that of non-competitive conditions, while adsorption of VN was higher on 100\%OH-SAMs. Concerning elution, retention of adsorbed VN was always higher than that of FN. For both proteins, functional presentation of cell-binding domains was more effective on the more hydrophilic 100\%OH-SAMs. This fact, coupled to the ability of this type of SAMs to selectively recruit and retain VN in the presence of competitive serum proteins, seems to correlate with the better cell response observed on these surfaces, as compared with hydrophobic 0\%OH(100\%CH
                        3)-SAMs. {\textcopyright} 2008 Elsevier Ltd. All rights reserved.
},
	keywords = {Adhesion, Adhesiveness, Adsorption, alkane, Animals, article, Biocompatible Materials, Biological Assay, bone marrow, Cattle, cell adhesion, Cell culture, Cell Movement, Cells, Cells, Cultured, cytology, cytoskeleton, Electric currents, Electron energy levels, enzyme linked immunosorbent assay, Fibronectin, Fibronectins, Focal adhesions, Functional groups, Functionalization, gold, human, human cell, Humans, hydrophilicity, hydrophobicity, Hydroxylation, isotope labeling, Mesenchymal stem cell, Mesenchymal Stem Cells, Microscopy, Confocal, Missile bases, Molecular interactions, monoclonal antibody, priority journal, Protein adsorption, protein analysis, protein binding, Protein Structure, Tertiary, Proteins, Self assembled monolayers, Self-assembled monolayers, stem cell, Stem cells, Surface Properties, thiol derivative, Vitronectin},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-55249093830\&partnerID=40\&md5=132fa4efe300c4f76764942bfe3bce30},
	author = {Barrias, C.C. and Martins, M.C.L. and Almeida-Porada, G. and Barbosa, M.A. and Granja, P.L.}
}
@article {2317,
	title = {Engineering endochondral bone: In vivo studies},
	journal = {Tissue Engineering - Part ATissue Eng. Part A},
	volume = {15},
	year = {2009},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: Scopusdoi: 10.1089/ten.tea.2008.0052Language of Original Document: EnglishCorrespondence Address: Teixeira, C. C.; Department of Basic Science and Craniofacial Biology, College of Dentistry, New York University, 345 East. 24th St., New York, NY 10010; email: ct40@nyu.eduChemicals/CAS: chitosan, 9012-76-4; Chitosan, 9012-76-4; MineralsReferences: Yelin, E., Callahan, L.F., The economic cost and social and psychological impact of musculoskeletal conditions. National Arthritis Data Work Groups (1995) Arthritis Rheum, 38, p. 1351;Service, R.F. Tissue engineers build new bone. Science 289,1498, 2000Dupraz, A., Delecrin, J., Moreau, A., Pilet, P., Passuti, N., Long-term bone response to particulate injectable ceramic (1998) J Biomed Mater Res, 42, p. 368; 
Gauthier, O., Khairoun, I., Bosco, J., Obadia, L., Bourges, X., Rau, C., Magne, D., Daculsi, G., Noninvasive bone replacement with a new injectable calcium phosphate biomaterial (2003) J Biomed Mater Res, 66 A, p. 47; 
Maroudas, A., Bayliss, M.T., Uchitel-Kaushansky, N., Schneiderman, R., Gilav, E., Aggrecan turnover in human articular cartilage: Use of aspartic acid racemization as a marker of molecular age (1998) Arch Biochem Biophys, 350, p. 61; 
Ferguson, C., Alpern, E., Miclau, T., Helms, J.A., Does adult fracture repair recapitulate embryonic skeletal formation? (1999) Mech Dev, 87, p. 57; 
Iwamoto, M., Higuchi, Y., Enomoto-Iwamoto, M., Kurisu, K., Koyama, E., Yeh, H., Rosenbloom, J., Pacifici, M., The role of ERG (ets related gene) in cartilage development (2001) Osteoarthr Cartil, 9 (SUPPL. A), pp. S41; 
Montufar-Solis, D., Nguyen, H.C., Nguyen, H.D., Horn, W.N., Cody, D.D., Duke, P.J., Using cartilage to repair bone: An alternative approach in tissue engineering (2004) Ann Biomed Eng, 32, p. 504; 
Pountos, I., Jones, E., Tzioupis, C., McGonagle, D., Giannoudis, P.V., Growing bone and cartilage: The role of mesenchymal stem cells (2006) J Bone Joint Surg Br, 88, p. 421; 
Valimaki, V.V., Yrjans, J.J., Vuorio, E.I., Aro, H.T., Molecular biological evaluation of bioactive glass microspheres and adjunct bone morphogenetic protein 2 gene transfer in the enhancement of new bone formation (2005) Tissue Eng, 11, p. 387; 
Huang, Y.C., Simmons, C., Kaigler, D., Rice, K.G., Mooney, D.J., Bone regeneration in a rat cranial defect with delivery of PEI-condensed plasmid DNA encoding for bone morphogenetic protein-4 (BMP-4) (2005) Gene Ther, 12, p. 418; 
Bikram, M., Fouletier-Dilling, C., Hipp, J.A., Gannon, F., Davis, A.R., Olmsted-Davis, E.A., West, J.L., Endochondral bone formation from hydrogel carriers loaded with BMP2-transduced cells (2007) Ann Biomed Eng, 35, p. 796; 
Alsberg, E., Anderson, K.W., Albeiruti, A., Rowley, J.A., Mooney, D.J., Engineering growing tissues (2002) Proc Natl Acad Sci USA, 99, p. 12025; 
Deckers, M.M., Karperien, M., van der Bent, C., Yamashita, T., Papapoulos, S.E., Lowik, C.W., Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation (2000) Endocrinology, 141, p. 1667; 
Petersen, W., Tsokos, M., Pufe, T., Expression of VEGF121 and VEGF165 in hypertrophic chondrocytes of the human growth plate and epiphyseal cartilage (2002) J Anat, 201, p. 153; 
Nakagawa, M., Kaneda, T., Arakawa, T., Morita, S., Sato, T., Yomada, T., Hanada, K., Hakeda, Y., Vascular endothelial growth factor (VEGF) directly enhances osteoclastic bone resorption and survival of mature osteoclasts (2000) FEBS Lett, 473, p. 161; 
Gerber, H.P., Vu, T.H., Ryan, A.M., Kowalski, J., Werb, Z., Ferrara, N., VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation (1999) Nat Med, 5, p. 623; 
Oliveira, S.M., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., (2007) Engineering endochondral bone: In vitro studies; 
Hasegawa, S., Ishii, S., Tamura, J., Furukawa, T., Neo, M., Matsusue, Y., Shikinami, Y., Nakamura, T., A 5-7 year in vivo study of high-strength hydroxyapatite/ poly(L-lactide) composite rods for the internal fixation of bone fractures (2006) Biomaterials, 27, p. 1327; 
Gauthier, O., Muller, R., von Stechow, D., Lamy, B., Weiss, P., Bouler, J.M., Aguado, E., Daculsi, G., In vivo bone regeneration with injectable calcium phosphate biomaterial: A three-dimensional micro-computed tomographic, biomechanical and SEM study (2005) Biomaterials, 26, p. 5444; 
Chan, C., Thompson, I., Robinson, P., Wilson, J., Hench, L., Evaluation of Bioglass/dextran composite as a bone graft substitute (2002) Int J Oral Maxillofac Surg, 31, p. 73; 
Trojani, C., Boukhechba, F., Scimeca, J.C., Vandenbos, F., Michiels, J.F., Daculsi, G., Boileau, P., Rochet, N., Ectopic bone formation using an injectable biphasic calcium phosphate/Si-HPMC hydrogel composite loaded with undifferentiated bone marrow stromal cells (2006) Biomaterials, 27, p. 3256; 
van Gaalen, S.M., Dhert, W.J.A., van den Muysenberg, A., Oner, F.C., van Blitterswijk, C., Verbout, A.J., de Bruijn, J.D., Bone tissue engineering for spine fusion: An experimental study on ectopic and orthotopic implants in rats (2004) Tissue Eng, 10, p. 231; 
Kim, H., Suh, H., Jo, S.A., Kim, H.W., Lee, J.M., Kim, E.H., Reinwald, Y., Jo, I., In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphate (2005) Biochem Biophys Res Commun, 332, p. 1053; 
Krebsbach, P.H., Kuznetsov, S.A., Satomura, K., Emmons, R.V.B., Rowe, D.W., Robey, P.G., Bone formation in vivo: Comparison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts (1997) Transplantation, 63, p. 1059; 
Musgrave, D.S., Bosch, P., Lee, J.Y., Pelinkovic, D., Ghivizzani, S.C., Whalen, J., Niyibizi, C., Huard, J., Ex vivogene therapy to produce bone using different cell types (2000) Clin Orthop Relat Res, 378, p. 290; 
Kojima, K., Bonassar, L.J., Ignotz, R.A., Syed, K., Cortiella, J., Vacanti, C.A., Comparison of tracheal and nasal chondrocytes for tissue engineering of the trachea (2003) Ann Thorac Surg, 76, p. 1884; 
van Susante, J.L., Buma, P., Homminga, G.N., van den Berg, W.B., Veth, R.P., Chondrocyte-seeded hydroxyapatite for repair of large articular cartilage defects: A pilot study in the goat (1998) Biomaterials, 19, p. 2367; 
Lu, J.X., Prudhommeaux, F., Meunier, A., Sedel, L., Guillemin, G., Effects of chitosan on rat knee cartilages (1999) Biomaterials, 20, p. 1937; 
Dorotka, R., Windberger, U., Macfelda, K., Bindreiter, U., Toma, C., Nehrer, S., Repair of articular cartilage defects treated by microfracture and a three-dimensional collagen matrix (2005) Biomaterials, 26, p. 3617; 
Marijnissen, W.J.C.M., van Osch, G.J.V.M., Aigner, J., Verwoerd-Verhoef, H.L., Verhaar, J.A.N., Tissueengineered cartilage using serially passaged articular chondrocytes. Chondrocytes in alginate, combined in vivo with a synthetic (E210) or biologic biodegradable carrier (DBM) (2000) Biomaterials, 21, p. 571; 
Case, N.D., Duty, A.O., Ratcliffe, A., Muller, R., Guldberg, R.E., Bone formation on tissue-engineered cartilage constructs in vivo: Effects of chondrocyte viability and mechanical loading (2003) Tissue Eng, 9, p. 587; 
Cancedda, R., Cancedda, F.D., Castagnola, P., Chondrocyte differentiation (1995) Int Rev Cytol, 159, p. 265; 
Hunziker, E.B., Mechanism of longitudinal bone-growth and its regulation by growth-plate chondrocytes (1994) Microsc Res Tech, 28, p. 505; 
Kirsch, T., Nah, H.D., Shapiro, I.M., Pacifici, M., Regulated production of mineralization-competent matrix vesicles in hypertrophic chondrocytes (1997) J Cell Biol, 137, p. 1149; 
Anderson, H.C., Molecular-biology of matrix vesicles (1995) Clin Orthop Relat Res, 314, p. 266; 
Yaylaoglu, M.B., Yildiz, C., Korkusuz, F., Hasirci, V., A novel osteochondral implant (1999) Biomaterials, 20, p. 1513; 
Garimella, R., Bi, X.H., Camacho, N., Sipe, J.B., Anderson, H.C., Primary culture of rat growth plate chondrocytes: An in vitro model of growth plate histotype, matrix vesicle biogenesis and mineralization (2004) Bone, 34, p. 961; 
Boskey, A., Camacho, N.P., FT-IR imaging of native and tissue-engineered bone and cartilage (2007) Biomaterials, 28, p. 2465; 
Jikko, A., Aoba, T., Murakami, H., Takano, Y., Iwamoto, M., Kato, Y., Characterization of the mineralization process in cultures of rabbit growth plate chondrocytes (1993) Dev Biol, 156, p. 372; 
Cadet, E.R., Gafni, R.I., McCarthy, E.F., McCray, D.R., Bacher, J.D., Barnes, K.M., Baron, J., Mechanisms responsible for longitudinal growth of the cortex: Coalescence of trabecular bone into cortical bone (2003) J Bone Joint Surg Am, 85 A, p. 1739; 
Lee, C., Grad, S., Wimmer, M., Alini, M., The influence of mechanical stimuli on articular cartilage tissue engineering (2006) Cartilage Tissue Eng, 2, p. 1; 
Trepczik, B., Lienau, J., Schell, H., Epari, D.R., Thompson, M.S., Hoffmann, J.E., Kadow-Romacker, A., Duda, G.N., Endochondral ossification in vitro is influenced by mechanical bending (2007) Bone, 40, p. 597; 
Tanck, E., Hannink, G., Ruimerman, R., Buma, P., Burger, E.H., Huiskes, R., Cortical bone development under the growth plate is regulated by mechanical load transfer (2006) J Anat, 208, p. 73; 
Dong, J., Uemura, T., Kojima, H., Kikuchi, M., Tanaka, J., Tateishi, T., Application of low-pressure system to sustain in vivo bone formation in osteoblast/porous hydroxyapatite composite (2001) DNLM, 17, p. 37; 
Stevens, M., Marini, R., Schaefer, D., Aronson, J., Langer, R., Shastri, V., In vivo engineering of organs: The bone bioreactor (2005) Proc Natl Acad Sci USA, 102, p. 11450; 
Rinaudo, M., Chitin and chitosan: Properties and applications (2006) Prog Polym Sci, 31, p. 603
},
	month = {2009///},
	pages = {635 - 643},
	abstract = {The use of biomaterials to replace lost bone has been a common practice for decades. More recently, the demands for bone repair and regeneration have pushed research into the use of cultured cells and growth factors in association with these materials. Here we report a novel approach to engineer new bone using a transient cartilage scaffold to induce endochondral ossification. Chondrocyte/chitosan scaffolds (both a transient cartilage scaffold- experimental-and a permanent cartilage scaffold-control) were prepared and implanted subcutaneously in nude mice. Bone formation was evaluated over a period of 5 months. Mineralization was assessed by Faxitron, micro computed tomography, backscatter electrons, and Fourier transform infrared spectroscopy analyses. Histological analysis provided further information on tissue changes in and around the implanted scaffolds. The deposition of ectopic bone was detected in the surface of the experimental implants as early as 1 month after implantation. After 3 months, bone trabeculae and bone marrow cavities were formed inside the scaffolds. The bone deposited was similar to the bone of the mice vertebra. Interestingly, no bone formation was observed in control implants. In conclusion, an engineered transient cartilage template carries all the signals necessary to induce endochondral bone formation in vivo. {\textcopyright} Copyright 2009, Mary Ann Liebert, Inc. 2009.},
	keywords = {animal, animal experiment, animal tissue, Animals, article, Biological materials, Biomaterials, Bone, Bone and Bones, Bone formation, bone marrow, bone mineralization, Bone repair, bone transplantation, Calcification, Physiologic, Cartilage, cartilage cell, chick embryo, chitosan, Chondrocytes, Computerized tomography, controlled study, Cultured cell, cytology, drug effect, Ectopic bones, ectopic tissue, electron, enchondral ossification, Endochondral ossification, Fourier transform infrared spectroscopy, Growth (materials), Growth factor, Histological analysis, histology, implantation, in vivo study, In-control, In-vivo, infrared spectroscopy, Ligaments, male, metabolism, methodology, Mice, Mice, Nude, micro-computed tomography, Microcomputed tomography, Microscopy, Electron, Scanning, mineral, Minerals, mouse, Mus, Mus musculus, nonhuman, Nude mice, nude mouse, ossification, physiology, priority journal, Prosthesis Implantation, Scaffolds, Scanning electron microscopy, Spectroscopic analysis, Spectroscopy, Spectroscopy, Fourier Transform Infrared, Tissue changes, Tissue engineering, tissue scaffold, Tissue Scaffolds, Tomography, trabecular bone, vertebra},
	isbn = {19373341 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-64549096039\&partnerID=40\&md5=d389cb8b10be8945007a5b6996b8896f},
	author = {Oliveira, S.M. and Mijares, D.Q. and Turner, G. and Amaral, I.F. and Barbosa, M.A. and Teixeira, C.C.}
}
@book {2295,
	title = {Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5627 LNCS},
	year = {2009},
	note = {Conference code: 77746Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02611-9_81Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica Divis{\~a}o de Sinal e Imagem, Campus FEUPReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, p. 13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Willemse, J., Kulikova, O., de Jong, H., Bisseling, T., A new whole-mount dna quantification method and the analysis of nuclear dna content in the stem-cell niche of arabidopsis roots (2008) The Plant Journal, 10, pp. 1365-1374; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Schmitt, M.H.O., Morphological multiscale decomposition of connected regions with emphasis on cell clusters (2009) Computer Vision and Image Understanding, 113, pp. 188-201; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. on Circuits and Systems I-Regul. Papers, 53 (11), pp. 2405-2414; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proceedings of the Int. Conference on Image Analysis and Processing, pp. 757-761; 
},
	month = {2009///},
	pages = {824 - 831},
	address = {Halifax, NS},
	abstract = {The study of cell nuclei is fundamental for plant cell Biology research. To obtain information at cellular level, researchers image cells{\textquoteright} nuclei which were modified with fluorescence proteins, through laser scanning confocal microscopy. These images are normally noisy and suffer from high background fluorescence, making grey-scale segmentation approaches inadequate for a usable detection. To obtain a successful detection even at low contrast we investigate the use of a particular convergence filter, the Symmetric Sliding Band filter (SSBF), for cell detection. This filter is based on gradient convergence and not intensity. As such it can detect low contrast cell nuclei which otherwise would be lost in the background noise. Due to the characteristics of cell nuclei morphology, a symmetry constrain is integrated in the filter which corrects some inadequate detections and results in a filter response that is more discriminative. We evaluate the use of this filter for cell nuclei detection on the Arabidopsis thaliana root tip, where the nuclei were stained using yellow fluorescence protein. The resulting cell nuclei detection precision is 89\%. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {Arabidopsis thaliana, Background noise, Band filters, Bioinformatics, Biology imaging, Cell detection, Cell membranes, cell nucleus, Cellular levels, confocal microscopy, Convergence filters, cytology, Filter response, Fluorescence, Fluorescence proteins, Fluorescent images, Image analysis, Laser scanning confocal microscopy, Low contrast, Plant cell culture, Plant cells, Positron emission tomography, Root tip, Yellow fluorescence proteins},
	isbn = {03029743 (ISSN); 3642026109 (ISBN); 9783642026102 (ISBN)},
	author = {Marcuzzo, M. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2297,
	title = {Fibronectin-mediated endothelialisation of chitosan porous matrices},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2009.06.056PubMed ID: 19615736Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; fibronectin, 86088-83-7; Biocompatible Materials; Chitosan, 9012-76-4; FibronectinsReferences: Moon, J.J., West, J.L., Vascularization of engineered tissues: approaches to promote angiogenesis in biomaterials (2008) Current Topics in Medicinal Chemistry, 8 (4), pp. 300-310;Blitterswijk, C.A., Moroni, L., Rouwkema, J., Siddappa, R., Sohier, J., Tissue engineering - an introduction (2008) Tissue engineering, , Blitterswijk C.A. (Ed), Elsevier; 
Ehrbar, M., Zeisberger, S.M., Raeber, G.P., Hubbell, J.A., Schnell, C., Zisch, A.H., The role of actively released fibrin-conjugated VEGF for VEGF receptor 2 gene activation and the enhancement of angiogenesis (2008) Biomaterials, 29 (11), pp. 1720-1729; 
Elbjeirami, W.M., West, J.L., Angiogenesis-like activity of endothelial cells co-cultured with VEGF-producing smooth muscle cells (2006) Tissue Engineering, 12 (2), pp. 381-390; 
Unger, R.E., Peters, K., Wolf, M., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Endothelialization of a non-woven silk fibroin net for use in tissue engineering: growth and gene regulation of hunian endothelial cells (2004) Biomaterials, 25 (21), pp. 5137-5146; 
Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials (2006) Biomaterials, 27 (31), pp. 5399-5408; 
Kirkpatrick, C.J., Unger, R.E., Krump-Konvalinkova, V., Peters, K., Schmidt, H., Kamp, G., Experimental approaches to study vascularization in tissue engineering and biomaterial applications (2003) Journal of Materials Science, 14 (8), pp. 677-681; 
Calonder, C., Matthew, H.W.T., Van Tassel, P.R., Adsorbed layers of oriented fibronectin: a strategy to control cell-surface interactions (2005) Journal of Biomedical Materials Research Part A, 75 A (2), pp. 316-323; 
Kouvroukoglou, S., Dee, K.C., Bizios, R., McIntire, L.V., Zygourakis, K., Endothelial cell migration on surfaces modified with immobilized adhesive peptides (2000) Biomaterials, 21 (17), pp. 1725-1733; 
Burdick, J.A., Khademhosseini, A., Langer, R., Fabrication of gradient hydrogels using a microfluidics/photopolymerization process (2004) Langmuir, 20 (13), pp. 5153-5156; 
Garcia, A.J., Get a grip: integrins in cell-biomaterial interactions (2005) Biomaterials, 26, pp. 7525-7529; 
Tomihata, K., Ikada, Y., In vitro and in vivo degradation of films of chitin and its deacetylated derivatives (1997) Biomaterials, 18 (7), pp. 567-575; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cells osteogenic differentiation and fibronectin adsorption on chitosan membranes: the effect of the degree of acetylation (2005) Journal of Biomedical Materials Research Part A, 75 A (2), pp. 387-397; 
Nettles, D.L., Elder, S.H., Gilbert, J.A., Potential use of chitosan as a cell scaffold material for cartilage tissue engineering (2002) Tissue Engineering, 8 (6), pp. 1009-1016; 
Oliveira, S.M., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., Engineering endochondral bone: in vitro studies (2009) Tissue Engineering, 15 (3), pp. 625-634; 
Freier, T., Koh, H.S., Kazazian, K., Shoichet, M.S., Controlling cell adhesion and degradation of chitosan films by N-acetylation (2005) Biomaterials, 26 (29), pp. 5872-5878; 
Wang, X.H., Cui, F.Z., Feng, Q.L., Li, J.C., Zhang, Y.H., Preparation and characterization of collagen/chitosan matrices as potential biomaterials (2003) Journal of Bioactive and Compatible Polymers, 18 (6), pp. 453-467; 
Chupa, J.M., Foster, A.M., Sumner, S.R., Madihally, S.V., Matthew, H.W.T., Vascular cell responses to polysaccharide materials: in vitro and in vivo evaluations (2000) Biomaterials, 21 (22), pp. 2315-2322; 
Chung, T.W., Lu, Y.F., Wang, S.S., Lin, Y.S., Chu, S.H., Growth of human endothelial cells on photochemically grafted Gly-Arg-Gly-Asp (GRGD) chitosans (2002) Biomaterials, 23 (24), pp. 4803-4809; 
Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., Adhesive protein interactions with chitosan: consequences for valve endothelial cell growth on tissue-engineering materials (2003) Journal of Biomedical Materials Research Part A, 67 A (2), pp. 538-547; 
Huang, Y., Onyeri, S., Siewe, M., Moshfeghian, A., Madihally, S.V., In vitro characterization of chitosan-gelatin scaffolds for tissue engineering (2005) Biomaterials, 26 (36), pp. 7616-7627; 
Huang, Y., Siewe, M., Madilhally, S.V., Effect of spatial architecture on cellular colonization (2006) Biotechnology and Bioengineering, 93 (1), pp. 64-75; 
Kirsebom, H., Aguilar, M.R., Roman, J.S., Fernandez, M., Prieto, M.A., Bondar, B., Macroporous scaffolds based on chitosan and bioactive molecules (2007) Journal of Bioactive and Compatible Polymers, 22 (6), pp. 621-636; 
Cao, W.L., Jing, D.H., Li, J.M., Gong, Y.D., Zhao, N.M., Zhang, X.F., Effects of the degree of deacetylation on the physicochemical properties and Schwann cell affinity of chitosan films (2005) Journal of Biomaterials Applications, 20 (2), pp. 157-177; 
Amaral, I.F., Sampaio, P., Barbosa, M.A., Three-dimensional culture of human osteoblastic cells in chitosan sponges: the effect of the degree of acetylation (2006) Journal of Biomedical Materials Research Part A, 76 A (2), pp. 335-346; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42 (8), pp. 3569-3580; 
Vachoud, L., Zydowicz, N., Domard, A., Formation and characterisation of a physical chitin gel (1997) Carbohydrate Research, 302 (3-4), pp. 169-177; 
Montembault, A., Viton, C., Domard, A., Physico-chemical studies of the gelation of chitosan in a hydroalcoholic medium (2005) Biomaterials, 26 (8), pp. 933-943; 
Amaral, I.F., Cordeiro, A.L., Sampaio, P., Barbosa, M.A., Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation (2007) Journal of Biomaterials Science Polymer Edition, 18 (4), pp. 469-485; 
Krump-Konvalinkova, V., Bittinger, F., Unger, R.E., Peters, K., Lehr, H.A., Kirkpatrick, C.J., Generation of human pulmonary microvascular endothelial cell lines (2001) Laboratory Investigation, 81 (12), pp. 1717-1727; 
Unger, R.E., Krump-Konvalinkova, V., Peters, K., Kirkpatrick, C.J., In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R (2002) Microvascular Research, 64 (3), pp. 384-397; 
Jaffe, E.A., Nachman, R.L., Becker, C.G., Minick, C.R., Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria (1973) J Clin Invest, 52 (11), pp. 2745-2756; 
O{\textquoteright}Brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) European Journal of Biochemistry, 267 (17), pp. 5421-5426; 
Teixeira, C.C., Hatori, M., Leboy, P.S., Pacifici, M., Shapiro, I.M., A rapid and ultrasensitive method for measurement of DNA, calcium and protein-content, and alkaline-phosphatase activity of chondrocyte cultures (1995) Calcified Tissue International, 56 (3), pp. 252-256; 
Barbosa, J.N., Amaral, I.F., {\'A}guas, A.P., Barbosa, M.A., Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds Journal of Biomedical Materials Research Part A, , in press, doi:10.1002/jbm.a.32499; 
Marshall, A.J., Barker, T., Sage, E.H., Hauch, K.D., Ratner, B.D., (2004) Pore size controls angiogenesis in subcutaneously implanted porous matrices. 7th World Biomaterials Congress; 2004, , Australian Society for Biomaterials Inc., Sydney, Australia p. 710; 
Unger, R.E., Sartoris, A., Peters, K., Motta, A., Migliaresi, C., Kunkel, M., Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials (2007) Biomaterials, 28 (27), pp. 3965-3976; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13 (13), pp. 3404-3413; 
Wendt, D., Marsano, A., Jakob, M., Heberer, M., Martin, I., Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity (2003) Biotechnology and Bioengineering, 84 (2), pp. 205-214; 
Santos, M.I., Fuchs, S., Gomes, M.E., Unger, R.E., Reis, R.L., Kirkpatrick, C.J., Response of micro- and macrovascular endothelial cells to starch-based fiber meshes for bone tissue engineering (2007) Biomaterials, 28 (2), pp. 240-248; 
Vestweber, D., Molecular mechanisms that control endothelial cell contacts (2000) Journal of Pathology, 190 (3), pp. 281-291; 
Mori, T., Irie, Y., Nishimura, S., Tokura, S., Matsuura, M., Okumura, M., Endothelial cell responses to chitin and its derivatives (1998) Journal of Biomedical Materials Research, 43 (4), pp. 469-472; 
Panetti, T.S., Hannah, D.F., Avraamides, C., Gaughan, J.P., Marcinkiewicz, Z., Huttenlocher, A., Extracellular matrix molecules regulate endothelial cell migration stimulated by lysophosphatidic acid (2004) Journal of Thrombosis and Haemostasis, 2 (9), pp. 1645-1656; 
Garcia, A.J., Boettiger, D., Integrin-fibronectin interactions at the cell-material interface: initial integrin binding and signaling (1999) Biomaterials, 20 (23-24), pp. 2427-2433; 
Danen, E.H.J., Sonnenberg, A., Integrins in regulation of tissue development and function (2003) Journal of Pathology, 200 (4), pp. 471-480; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) Journal of Biomedical Materials Research Part A, 66 A (2), pp. 247-259; 
Renner, L., Jorgensen, B., Markowski, M., Salchert, K., Werner, C., Pompe, T., Control of fibronectin displacement on polymer substrates to influence endothelial cell behaviour (2004) Journal of Materials Science-Materials in Medicine, 15 (4), pp. 387-390; 
Garcia, A.J., Vega, M.D., Boettiger, D., Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation (1999) Molecular Biology of the Cell, 10 (3), pp. 785-798; 
Koenig, A.L., Gambillara, V., Grainger, D.W., Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates (2003) Journal of Biomedical Materials Research Part A, 64 A (1), pp. 20-37; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding (2004) Biomaterials, 25, pp. 5947-5954; 
Pompe, T., Kobe, F., Salchert, K., Jorgensen, B., Oswald, J., Werner, C., Fibronectin anchorage to polymer substrates controls the initial phase of endothelial cell adhesion (2003) Journal of Biomedical Materials Research Part A, 67 A (2), pp. 647-657
},
	month = {2009///},
	pages = {5465 - 5475},
	abstract = {Chitosan (Ch) porous matrices were investigated regarding their ability to be colonized by human microvascular endothelial cells (HPMEC-ST1.6R cell line) and macrovascular endothelial cells namely HUVECs. Specifically we assessed if previous incubation of Ch in a fibronectin (FN) solution was effective in promoting endothelial cell (EC) adhesion to Ch matrices with different degrees of acetylation (DAs). Upon FN physiadsorption, marked differences were found between the two DAs investigated, namely DA 4\% and 15\%. While cell adhesion was impaired on Ch with DA 15\%, ECs were able to not only adhere to Ch with DA 4\%, but also to spread and colonize the scaffolds, with retention of the EC phenotype and angiogenic potential. To explain the observed differences between the two DAs, protein adsorption studies using                        125I-FN and immunofluorescent labelling of FN cell-binding domains were carried out. In agreement with the higher cell numbers found, scaffolds with DA 4\% revealed a higher number of exposed FN cell-binding domains as well as greater ability to adsorb FN and to retain and exchange adsorbed FN in the presence of competitive proteins. These findings suggest that the DA is a key parameter modulating EC adhesion to FN-coated Ch by influencing the adsorbed protein layer. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.
},
	keywords = {Acetylation, Adhesion, Adsorption, angiogenesis, article, Biocompatible Materials, Blood vessel prostheses, cell adhesion, Cell culture, cell invasion, Cell Line, Cell lines, Cell membranes, Cell number, cell proliferation, Cell size, cell spreading, cell survival, Cell-binding domain, Chitin, Chitin/chitosan, chitosan, colony formation, controlled study, Degrees of acetylation, Electric conductivity measurement, Electric currents, Endothelial cell, Endothelial Cells, Endothelialisation, endothelium, endothelium cell, epithelization, Fibronectin, Fibronectins, human, human cell, Human microvascular endothelial cells, Humans, immunofluorescence test, Key parameters, Labels, materials testing, Matrix algebra, Neovascularization, Physiologic, phenotype, porosity, Porous matrixs, priority journal, Protein adsorption, Protein layers, Scaffold, Scaffolds, Self assembly, Tissue engineering, tissue scaffold, umbilical vein},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68549107574\&partnerID=40\&md5=9babb57c1a4f2ae6eb7e7e0643bffe48},
	author = {Amaral, I.F. and Unger, R.E. and Fuchs, S. and Mendon{\c c}a, A.M. and Sousa, S.R. and Barbosa, M.A. and P{\^e}go, A.P. and Kirkpatrick, C.J.}
}
@article {2296,
	title = {Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.04.021PubMed ID: 19427930Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; chitosan, 9012-76-4; imidazole, 1467-16-9, 288-32-4; Biocompatible Materials; Buffers; Chitosan, 9012-76-4; DNA, 9007-49-2; Drug CarriersReferences: Kirkpatrick, C.J., Fuchs, S., Peters, K., Brochhausen, C., Hermanns, M.I., Unger, R.E., Visions for regenerative medicine: interface between scientific fact and science fiction (2006) Artif Organs, 30, pp. 822-827;Sim{\~o}es, S., Filipe, A., Faneca, H., Mano, M., Penacho, N., Duzgunes, N., Cationic liposomes for gene delivery (2005) Expert Opin Drug Deliv, 2, pp. 237-254; 
Lungwitz, U., Breunig, M., Blunk, T., Gopferich, A., Polyethylenimine-based non-viral gene delivery systems (2005) Eur J Pharm Biopharm, 60, pp. 247-266; 
Filion, M.C., Phillips, N.C., Major limitations in the use of cationic liposomes for DNA delivery (1998) Int J Pharm, 162, pp. 159-170; 
Lv, H., Zhang, S., Wang, B., Cui, S., Yan, J., Toxicity of cationic lipids and cationic polymers in gene delivery (2006) J Control Release, 114, pp. 100-109; 
Itaka, K., Ohba, S., Miyata, K., Kawaguchi, H., Nakamura, K., Takato, T., Bone regeneration by regulated in vivo gene transfer using biocompatible polyplex nanomicelles (2007) Mol Ther, 15, pp. 1655-1662; 
Borchard, G., Chitosans for gene delivery (2001) Adv Drug Deliv Rev, 52, pp. 145-150; 
Khalil, I.A., Kogure, K., Akita, H., Harashima, H., Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery (2006) Pharmacol Rev, 58, pp. 32-45; 
Behr, J.P., The proton sponge: a trick to enter cells the viruses did not exploit (1997) Chimia, 51, pp. 34-36; 
Kim, T.H., Kim, S.I., Akaike, T., Cho, C.S., Synergistic effect of poly(ethylenimine) on the transfection efficiency of galactosylated chitosan/DNA complexes (2005) J Control Release, 105, pp. 354-366; 
Midoux, P., Monsigny, M., Efficient gene transfer by histidylated polylysine/pDNA complexes (1999) Bioconjug Chem, 10, pp. 406-411; 
Benns, J.M., Choi, J.S., Mahato, R.I., Park, J.S., Kim, S.W., pH-sensitive cationic polymer gene delivery vehicle: N-Ac-poly(l-histidine)-graft-poly(l-lysine) comb shaped polymer (2000) Bioconjug Chem, 11, pp. 637-645; 
Putnam, D., Gentry, C.A., Pack, D.W., Langer, R., Polymer-based gene delivery with low cytotoxicity by a unique balance of side-chain termini (2001) Proc Natl Acad Sci USA, 98, pp. 1200-1205; 
Dubruel, P., Christiaens, B., Vanloo, B., Bracke, K., Rosseneu, M., Vandekerckhove, J., Physicochemical and biological evaluation of cationic polymethacrylates as vectors for gene delivery (2003) Eur J Pharm Sci, 18, pp. 211-220; 
Dubruel, P., Dekie, L., Schacht, E., Poly-l-glutamic acid derivatives as multifunctional vectors for gene delivery. Part A. Synthesis and physicochemical evaluation (2003) Biomacromolecules, 4, pp. 1168-1176; 
Kim, T.H., Ihm, J.E., Choi, Y.J., Nah, J.W., Cho, C.S., Efficient gene delivery by urocanic acid-modified chitosan (2003) J Control Release, 93, pp. 389-402; 
Dubruel, P., Christiaens, B., Rosseneu, M., Vandekerckhove, J., Grooten, J., Goossens, V., Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery (2004) Biomacromolecules, 5, pp. 379-388; 
Juusola, P., Minkkinen, P., Leino, L., Laihia, J.K., Determination of the dissociation constants of urocanic acid isomers in aqueous solutions (2007) Monatsh Chem, 138, pp. 951-965; 
Roberts, J.D., Yu, C., Flanagan, C., Birdseye, T.R., A nitrogen-15 nuclear magnetic resonance study of the acid-base and tautomeric equilibria of 4-substituted imidazoles and its relevance to the catalytic mechanism of alfa-lytic protease (1982) J Am Chem Soc, 104, pp. 3945-3949; 
Roberts, G.A.F., (1992) Chitin chemistry, , Palgrave Macmillan, London; 
Nakagawa, Y., Murai, T., Hasegawa, C., Hirata, M., Tsuchiya, T., Yagami, T., Endotoxin contamination in wound dressings made of natural biomaterials (2003) J Biomed Mater Res B Appl Biomater, 66, pp. 347-355; 
(1987) Guideline on validation of the limulus amebocyte lysate test as an end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices, , U.S. Department of Health and Human Services, Public Health Service, Food and Drug Administration; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., Molecular parameters of chitosans depolymerized with the aid of papain (1996) Carbohydr Polym, 29, pp. 63-68; 
Sehgal, D., Vijay, I.K., A method for the high-efficiency of water-soluble carbodiimide-mediated amidation (1994) Anal Biochem, 218, pp. 87-91; 
Leong, K.W., Mao, H.Q., Truong-Le, V.L., Roy, K., Walsh, S.M., August, J.T., DNA-polycation nanospheres as non-viral gene delivery vehicles (1998) J Control Release, 53, pp. 183-193; 
Lee, M., Nah, J.W., Kwon, Y., Koh, J.J., Ko, K.S., Kim, S.W., Water-soluble and low molecular weight chitosan-based plasmid DNA delivery (2001) Pharm Res, 18, pp. 427-431; 
van Kuppeveld, F.J., van der Logt, J.T., Angulo, A.F., van Zoest, M.J., Quint, W.G., Niesters, H.G., Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification (1992) Appl Environ Microbiol, 58, pp. 2606-2615; 
van Kuppeveld, F.J., van der Logt, J.T., Angulo, A.F., van Zoest, M.J., Quint, W.G., Niesters, H.G., Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification (1993) Appl Environ Microbiol, 59, p. 655; 
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays (1983) J Immunol Methods, 65, pp. 55-63; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Godbey, W.T., Ku, K.K., Hirasaki, G.J., Mikos, A.G., Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency (1999) Gene Ther, 6, pp. 1380-1388; 
Pouton, C.W., Lucas, P., Thomas, B.J., Uduehi, A.N., Milroy, D.A., Moss, S.H., Polycation-DNA complexes for gene delivery: a comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids (1998) J Control Release, 53, pp. 289-299; 
Mao, H.Q., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency (2001) J Control Release, 70, pp. 399-421; 
Poole, B., Ohkuma, S., Effect of weak bases on the intralysosomal pH in mouse peritoneal-macrophages (1981) J Cell Biol, 90, pp. 665-669; 
Bowman, E.J., Siebers, A., Altendorf, K., Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells (1988) Proc Natl Acad Sci USA, 85, pp. 7972-7976; 
van Deurs, B., Holm, P.K., Sandvig, K., Inhibition of the vacuolar H+ATPase with bafilomycin reduces delivery of internalized molecules from mature multivesicular endosomes to lysosomes in HEp-2 cells (1996) Eur J Cell Biol, 69, pp. 343-350; 
Merdan, T., Kopecek, J., Kissel, T., Prospects for cationic polymers in gene and oligonucleotide therapy against cancer (2002) Adv Drug Deliv Rev, 54, pp. 715-758; 
Mansouri, S., Lavigne, P., Corsi, K., Benderdour, M., Beaumont, E., Fernandes, J.C., Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy (2004) Eur J Pharm Biopharm, 57, pp. 1-8; 
Erbacher, P., Zou, S.M., Bettinger, T., Steffan, A.M., Remy, J.S., Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability (1998) Pharm Res, 15, pp. 1332-1339; 
Kiang, T., Wen, J., Lim, H.W., Leong, K.W., The effect of the degree of chitosan deacetylation on the efficiency of gene transfection (2004) Biomaterials, 25, pp. 5293-5301; 
Ishii, T., Okahata, Y., Sato, T., Mechanism of cell transfection with plasmid/chitosan complexes (2001) BBA-Biomembranes, 1514, pp. 51-64; 
Lavertu, M., Methot, S., Tran-Khanh, N., Buschmann, M.D., High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation (2006) Biomaterials, 27, pp. 4815-4824; 
de Smedt, S.C., Demeester, J., Hennink, W.E., Cationic polymer based gene delivery systems (2000) Pharm Res, 17, pp. 113-126; 
Kichler, A., Leborgne, C., Coeytaux, E., Danos, O., Polyethylenimine-mediated gene delivery: a mechanistic study (2001) J Gene Med, 3, pp. 135-144; 
Wattiaux, R., Laurent, N., Wattiaux-De Coninck, S., Jadot, M., Endosomes, lysosomes: their implication in gene transfer (2000) Adv Drug Deliv Rev, 41, pp. 201-208; 
Pelkmans, L., Helenius, A., Endocytosis via caveolae (2002) Traffic, 3, pp. 311-320; 
Felgner, P.L., Gadek, T.R., Holm, M., Roman, R., Chan, H.W., Wenz, M., Lipofection-a highly efficient, lipid-mediated DNA-transfection procedure (1987) Proc Natl Acad Sci USA, 84, pp. 7413-7417; 
Conner, S.D., Schmid, S.L., Regulated portals of entry into the cell (2003) Nature, 422, pp. 37-44; 
Koping-Hoggard, M., Tubulekas, I., Guan, H., Edwards, K., Nilsson, M., Varum, K.M., Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo (2001) Gene Ther, 8, pp. 1108-1121; 
Boussif, O., Zanta, M.A., Behr, J.P., Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold (1996) Gene Ther, 3, pp. 1074-1080
},
	month = {2009///},
	pages = {2995 - 3006},
	abstract = {Chitosan was functionalized with imidazole moieties (CHimi) with the aim of improving its buffering capacity and promoting the endosomal escape ability of chitosan-DNA complexes, ultimately increasing their transfection efficiency. 5.6\%, 12.9\% and 22.1\% of the glucosamine residues of chitosan were substituted. Complexes with different molar ratios of primary amines to DNA phosphate anion (N/P) were prepared by a coacervation method. For an N/P \&gt; 3, CHimi polymers are able to complex electrostatically with DNA and condense it into positively charged nanostructures (average size 260 nm and zeta potential +16 mV at pH 5.5). In the concentration range 2.5-100 μg ml},
	keywords = {article, beta galactosidase, Biocompatible Materials, Buffers, Cell Line, cell transport, Chemical structure, chitosan, coacervation, complex formation, controlled study, Cytotoxicity, Diffusion, DNA, Drug Carriers, Drug Compounding, endosome, Gene delivery, Gene transfer, genetic transfection, human, human cell, Humans, imidazole, Kidney, materials testing, nanomaterial, nanoparticle, priority journal, protein expression, proton transport, Tissue engineering, Transfection},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70349162856\&partnerID=40\&md5=fb6672dd0cf06d6ced21eec115b597d2},
	author = {Moreira, C. and Oliveira, H. and Pires, L.R. and Sim{\~o}es, S. and Barbosa, M.A. and P{\^e}go, A.P.}
}
@article {2291,
	title = {Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2009.09.010PubMed ID: 19783294Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R.M.; INEB, Biomaterials Division, NEWTherapies Group, R. Campo Alegre, 823, 4150-180 Porto, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: Calcium-Binding Proteins; Gold, 7440-57-5; Immunoglobulin G; Intercellular Signaling Peptides and Proteins; Membrane Proteins; Receptors, Fc; Receptors, Notch; Recombinant Fusion Proteins; Serrate proteins, 134324-36-0References: Artavanis-Tsakonas, S., Rand, M.D., Lake, R.J., Notch signaling: cell fate control and signal integration in development (1999) Science, 284, pp. 770-776;Leong, K., Karsan, A., Recent insights in the role of notch signaling in tumorigenesis (2006) Blood, 107 (6), pp. 2223-2233; 
Chiba, S., Concise review: notch signaling in stem cell systems (2006) Stem Cells, 24, pp. 2437-2447; 
Lowell, S., Benchoua, A., Heavey, B., Smith, A.G., Notch promotes neural lineage entry by pluripotent embryonic stem cells (2006) PLoS Biol, 4 (5); 
Hellstr{\"o}m, M.P.L., Hofmann, J.J., Wallgard, E., Coultas, L., Lindblom, P., Alva, J., Dll4 signalling through notch1 regulates formation of tip cells during angiogenesis (2007) Nature, 15 (445), pp. 776-780; 
Hofmeister, C., Zhang, J., Knight, K.L., Le, P., Stiff, P.J., Ex vivo expansion of umbilical cord blood stem cells for transplantation: growing knowledge from the hematopoietic niche (2007) Bone Marrow Transplant, 39 (1), pp. 11-23; 
Hilton, M., Tu, X., Wu, X., Bai, S., Zhao, H., Kobayashi, T., F. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation (2008) Nat Med, 14 (3), pp. 306-314; 
Stylianou, S., Clarke, R.B., Brennan, K., Aberrant activation of notch signaling in human breast cancer (2006) Cancer Res, 66 (3), pp. 1517-1525; 
Demarest, R., Ratti, F., Capobianco, A.J., It{\textquoteright}s T-ALL about notch (2008) Oncogene, 27 (38), pp. 5082-5091; 
Fre, S., Huyghe, M., Mourikis, P., Robine, S., Louvard, D., Artavanis-Tsakonas, S., Notch signals control the fate of immature progenitor cells in the intestine (2005) Nature, 16 (435), pp. 964-968; 
Fischer, A., Gessler, M., Delta-notch-and then? Protein interactions and proposed modes of repression by Hes and Hey bHLH factors (2007) Nucleic Acids Res, 35 (14), pp. 4583-4596; 
Zweidler-McKay, P.A., He, Y., Xu, L., Rodriguez, C.G., Karnell, F.G., Carpenter, A.C., Notch signaling is a potent inducer of growth arrest and apoptosis in a wide range of B-cell malignancies (2005) Blood, 106 (12), pp. 3898-3906; 
Beckstead, B., Santosa, D.M., Giachelli, C.M., Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation (2006) J Biomed Mater Res, 79 A, pp. 94-103; 
Varnum-Finney, B., Wu, L., Yu, M., Brashem-Stein, C., Staats, S., Flowers, D., Immobilization of Notch ligand, Delta-1, is required for induction of notch signaling (2000) J Cell Sci, 113 (23), pp. 4313-4318; 
Delaney, C., Varnum-Finney, B., Aoyama, K., Brashem-Stein, C., Bernstein, I.D., Dose-dependent effects of the notch ligand delta1 on ex vivo differentiation and in vivo marrow repopulating ability of cord blood cells (2005) Blood, 106, pp. 2693-2699; 
Lefort, N., Bennea, C., Leli{\`e}vrea, J.D., Dorivala, C., Balboa, M., Sakanoc, S., Short exposure to notch ligand delta-4 is sufficient to induce T-cell differentiation program and to increase the T cell potential of primary human CD34+ cells (2006) Exp Hematol, 34, pp. 1720-1729; 
Taqvi, S., Dixit, L., Krishnendu, R., Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells (2006) J Biomed Mater Res, 79 A, pp. 689-697; 
Bernstein, I., Delaney, C., Dallas, M., Heimfeld, S., Varnum-Finney, B., Notch signaling in hematopoiesis: implication for improving stem cell transplantation (2008) Biol Blood Marrow Transplant, 14, pp. 94-99; 
De Smedt, M., Hoebeke, I., Reyvoet, K., Leclercq, G., Plum, J., Different thresholds of~Notch signaling bias human precursor cells toward B-, NK-, monocytic/dendritic-, or T-cell lineage in thymus microenvironment (2005) Blood, 106, pp. 3498-3506; 
Mrksich, M., What can surface chemistry do for cell biology? (2002) Curr Opin Chem Biol, 6, pp. 794-797; 
France, R., Short, R.D., Dawsonb, R.A., MacNeilb, S., Attachment of human keratinocytes to plasma copolymers of acrylic acid/octa-1,7-diene and allyl amine/octa-1,7-diene (1998) J Mater Chem, 81 (1), pp. 37-42; 
Neves, H., Weerkamp, F., Gomes, A.C., Naber, B., Gameiro, P., Becker, J.L., Effects of delta1 and jagged1 on early human hematopoiesis: correlation with expression of notch signaling-related genes in CD34+ Cells (2006) Stem Cells, 24, pp. 1328-1337; 
Sokolova, V., Epple, M., Inorganic nanoparticles as carriers of nucleic acids into cells (2008) Angewandte Chemie, 47, pp. 1382-1395; 
Yao, J., Duan, L., Fan, M., Yuan, J., Wu, X., Notch1 induces cell cycle arrest and apoptosis in human cervical cancer cells: involvement of nuclear factor kappa B inhibition (2007) Int J Gynecol Cancer, 17, pp. 502-510; 
Chadwick, N., Nostro, M.C., Baron, M., Mottram, R., Brady, G., Buckle, A., Notch signaling induces apoptosis in primary human CD34+ hematopoietic progenitor cells (2007) Stem Cells, 25, pp. 203-210; 
Love, J., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1169; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Martins, M.C.L., Ratner, B., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl- terminated self-assembled monolyers (2003) J Biomed Mater Res, 67 (A), pp. 158-171; 
Martins, M.C.L., Curtins, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res A, 88 (1), pp. 162-173; 
Barrias, C.C., Martins, M.C.L., Bidarra, S., S{\'a} Miranda, M.C., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: effect of the surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
Iodine-125. A guide to radioiodination techniques. In: techniques I-Agtr, ed.: Little Chalfort: Amersham Life Science 1993Horbett, T., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, pp. 183-214. , DF W. (Ed), CRC Press Inc, Harcover; 
Ohishi, K., Varnum-Finney, B., Bernstein, I.D., Delta-1 enhances marrow and thymus repopulating ability of human CD34+ CD38- cord blood cells (2002) J Clin Invest, 110, pp. 1165-1174; 
Li, L., Chen, S., Zheng, J., Ratner, B.D., Jiang, S., Protein adsorption on oligo(ethylene glycol)-terminated alkanethiolate self-assembled monolayers: the molecular basis for nonfouling behavior (2005) J Phys Chem B, 109 (7), pp. 2934-2941; 
Tohda, S., Murata-Ohsawa, M., Sakano, S., Nara, N., Notch ligands, Delta-1 and Delta-4 supress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines (2003) Int J Oncol, 22 (5), pp. 1073-1079; 
Tohda, S.K.H., Murakami, N., Sakano, S., Nara, N., Diverse effects of the Notch ligands Jagged1 and Delta1 on the growth and differentiation of primary acute myeloblastic leukemia cells (2005) Exp Hematol, 33, pp. 558-563; 
Aoyama, K., Delaney, C., Varnun-Finney, B., Kohn, A.D., Moon, R.T., Bernstein, I.D., The interaction of the Wnt and notch pathways modulates natural killer versus T cell differentiation (2007) Stem Cells, 25, pp. 2488-2497; 
Beckstead B, Tung JC, Liang KJ, Tavakkol Z, Usui ML, Olerud JE, et~al. Methods to promote notch signaling at the biomaterial interface and evaluation in a rafted organ culture model. J Biomed Mater Res A, in press. [Epub ahead of print]Semler, E., Dasgupta, A., Moghe, P.V., Cytomimetic engineering of hepatocyte morphogenesis and function by substrate-based presentation of acellular E-cadherin (2005) Tissue Eng, 11 (5-6), pp. 734-750; 
Zhao, Z., Matsui, H., Accurate immobilization of antibody-functionalized peptide nanotubes on protein-patterned arrays by optimizing their ligand-receptor interactions (2007) Small, 3 (8), pp. 1390-1393; 
Longo, G., Szleifer, Ligand-receptor interactions in tethered polymer layers (2005) Langmuir, 21, pp. 11342-11351; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption and clotting time of pHEMA hydrogels modified with C18 ligands to adsorb albumin selectively and reversibly (2009) Biomaterials, 30 (29), pp. 5541-5551
},
	month = {2009///},
	pages = {6879 - 6887},
	abstract = {Notch signaling is a key mechanism during mammal development and stem cell regulation. This study aims to target and control Notch signaling by ligands immobilization using self-assembled monolayers (SAMs) as model surfaces. Non-fouling substrates were prepared by immersion of gold substrates in (1-Mercapto-11-undecyl)tetra(ethylene glycol) thiol solutions. These surfaces were activated with N,N'-carbonyldiimidazole (CDI) at different concentrations (0, 0.03, 0.3, 3 and 30 mg/ml) and an anti-human IgG, Fc specific fragment antibody (Ab) was covalently bound to EG4-SAMs to guarantee the correct exposure of the Notch ligand Jagged-1/Fc chimera (Jag-1). The presence of Ab and Jag-1 was confirmed by radiolabeling, X-ray photoelectron spectroscopy (XPS), ellipsometry and ELISA. The biological activity of Jag-1-Ab-SAMs was assessed by real-time PCR for Hes-1 family gene expression, a Notch pathway target gene, in HL-60 cell line. Results have shown an increase of the amount of immobilized Ab with increasing surface activator concentrations. Jag-1 concentration also increases with Ab concentration. Interestingly, a higher Jagged-1 exposure and fold increase in Hes-1 expression were obtained for surfaces activated with the lowest concentration of CDI (0.03 mg/ml). These results illustrate the great importance of ligands orientation and exposure, when compared with density. This investigation brings new insights into Notch signaling mechanisms. In particular, Jag-1-Ab-SAMs have shown to be adequate model surfaces to study Notch pathway activation and may provide a basis to develop new interfaces in biomaterials to control Notch mechanism in different cell systems. {\textcopyright} 2009 Elsevier Ltd.},
	keywords = {Activator concentration, Adequate models, Adsorption, Animals, article, Bioactivity, Biological activities, Biological materials, biomaterial, Calcium-Binding Proteins, Carbonyldiimidazole, Cell culture, Cell Line, Cell membranes, cell strain HL 60, Cell system, Chemical activation, Concentration (process), Concentration of, controlled study, Covalently bound, Ethylene, Ethylene Glycol, gene expression, gold, Gold substrates, HL-60 cell line, human, human cell, Human IgG, Humans, Immunoassay, immunoglobulin Fc fragment, Immunoglobulin G, immunoglobulin G antibody, Intercellular Signaling Peptides and Proteins, Jagged1, Ligands, Mammalia, Membrane Proteins, Model surface, Molecular Structure, Nanostructured materials, Nanostructured surfaces, Nonfouling, Notch receptor, Notch signaling, Photoelectron spectroscopy, priority journal, Protein adsorption, protein immobilization, Radiolabeling, Real-time PCR, Receptors, Fc, Receptors, Notch, Recombinant Fusion Proteins, Sams, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, signal transduction, Signaling, stem cell, Surface Properties, Target genes, Tetra, transcription factor HES 1, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70350188310\&partnerID=40\&md5=6c55d29cf8ae55288f3e87880171ec70},
	author = {Gon{\c c}alves, R.M. and Martins, M.C.L. and Almeida-Porada, G. and Barbosa, M.A.}
}
@article {2325,
	title = {Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {88},
	year = {2009},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31849PubMed ID: 18286636Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomaterials, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: carbonyldiimidazole, 530-62-1; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; imidazole, 1467-16-9, 288-32-4; leucine, 61-90-5, 7005-03-0; lysine, 56-87-1, 6899-06-5, 70-54-2; tetraethylene glycol, 112-60-7; Ethylene Glycol, 107-21-1; Heparin, 9005-49-6; Leucine, 61-90-5; Lysine, 56-87-1; PeptidesReferences: Dailey J. Blood. Ipswich, MA, USA: Medical Consulting Group; 1998Section, V., Chronic intermittent haemodialysis and prevention of clotting in the extracorporal system (2002) Nephrol Dialysis Transplant, 17, pp. 63-71;Zhang, Y.H., Singh, V.K., Yang, V.C., Novel approach for optimizing the capacity and efficacy of a protamine filter for clinical extracorporeal heparin removal (1998) Asaio J, 44, pp. M368-M373; 
Tao, W., Deyo, D.J., Brunston, R.L., Vertrees, R.A., Zwischenberger, J.B., Extracorporeal heparin adsorption following cardiopulmonary bypass with a heparin removal device - An alternative to protamine (1998) Critical Care Med, 26, pp. 1096-1102; 
Ameer, G.A., Barabino, G., Sasisekharan, R., Harmon, W., Cooney, C.L., Langer, R., Ex vivo evaluation of a Taylor-Couette flow, immobilized heparinase I device for clinical application (1999) Proc Natl Acad Sci USA, 96, pp. 2350-2355; 
Ostuni, E., Yan, L., Whitesides, G.M., The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver (1999) Colloids Surf B-Biointerfaces, 15, pp. 3-30; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers that present oligo(ethylene glycol) groups to control the interactions of proteins with surfaces. Poly(Ethylene Glycol) (1997) ACS Symposium Series, 680, pp. 361-373; 
Cardin, A.D., Weintraub, H.J.R., Molecular modeling of proteinglycosaminoglycan interactions (1989) Arteriosclerosis, 9, pp. 21-32; 
Kricheldorf, H.R., (1987) α-Amino Acid N-Carboxyanhydrides and Related Heterocycles, , New York: Springer Verlag;; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolayers (2003) J Biomed Mater Res A, 67, pp. 158-171; 
Lee, H.J., Nedelkov, D., Corn, R.M., Surface plasmon resonance imaging measurements of antibody arrays for the multiplexed detection of low molecular weight protein biomarkers (2006) Anal Chem, 78, pp. 6504-6510; 
Roeges, N.P.G., (1994) A Guide to the Complete Interpretation of Infrared Spectra of Organic Structures, , England: Wiley;; 
Li, L.Y., Chen, S.F., Zheng, J., Ratner, B.D., Jiang, S.Y., Protein adsorption on oligo(ethylene glycol)-terminated alkanethiolate self-assembled monolayers: The molecular basis for non-fouling behavior (2005) J Phys Chem B, 109, pp. 2934-2941; 
Beamson, G., High, B.D., (1992) Resolution XPS of Organic Polymers, , Chichester: Wiley;; 
Sousa, S.R., Moradas, F.P., Saramago, B., Viseu, M.L., Barbosa, M.A., Human serum albumin adsorption on TiO2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies: Tables and Charts, , Chichester: Wiley;; 
Capila, I., Linhardt, R.J., Heparin - Protein interactions (2002) Angewandte Chemie-Int Ed, 41, pp. 391-112; 
Snell, C.R.F.G., Kinetics and thermodynamics of the alpha Helix - β Transconformation of poly(L-lysine) and L-leucine copolymers. A compensation phenomenon (1973) Biochemistry, 12, pp. 1017-1025; 
Hearn, M.T.W., 1,1'-Carbonyldiimidazole-mediated immobilization of enzymes and affinity ligands (1987) Methods Enzymol, 135, pp. 102-117; 
Stollner, D., Scheller, F.W., Warsinke, A., Activation of cellulose membranes with 1,1'-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors (2002) Anal Biochem, 304, pp. 157-165
},
	month = {2009///},
	pages = {162 - 173},
	abstract = {Systemic heparinization, used during haemodialysis to prevent blood clotting on the extracorporeal circuit, leads to a high incidence of hemorrhagic complications. The adverse reactions associated with heparin neutralization using protamine sulphate justify the development of an alternative system for blood deheparinization. The main objective of this work is to design nanostructured surfaces with the capacity to bind heparin from blood in a selective way. A heparin-binding polypeptide, composed of L-lysine and L-leucine (pKL), was synthesized and immobilized, in different concentrations, onto self-assembled monolayers (SAMs) terminated with tetra(ethylene-glycol) (EG4 SAMs). Immobilization was performed using a fixed concentration of pKL after surface activation to different degrees using a range of CDI (N,N'-carbonyldiimidazole) concentrations. Results demonstrated that the presence of pKL increases heparin adsorption to EG4-SAMs, independently of the pKL concentration and the way of immobilization (adsorption or covalent bound). Selectivity towards heparin was successfully achieved on SAMs with low concentrations of immobilized pKL (9-17\% of pKL). Surfaces were characterized using ellipsometry, contact angle measurements, Fourier transform infrared reflection absorption spectroscopy (IRAS), atomic force microscopy, and X-ray photoelectron spectroscopy. Heparin adsorption was assessed using IRAS and N-sulphonate-                        35S-heparin. Therefore, this study could give a good contribution for the design of blood deheparinization devices. {\textcopyright} 2008 Wiley Periodicals, Inc.
},
	keywords = {Absorption spectroscopy, Adsorption, Alternative systems, Amines, Amino acids, Angle measurement, article, Atomic force microscopy, Atomic forces, Atomic spectroscopy, Binding peptides, Blood, Blood clotting, Carbonyl diimidazole, Carbonyldiimidazole, Contact angle, deheparinization, device, Dialysis, Electron energy levels, ellipsometry, Ethylene, Ethylene Glycol, Fourier Transform Infrared, Fourier transforms, Glycols, Haemodialysis, heparin, heparin binding protein, Heparinization, High incidences, Humans, imidazole, immobilization, infrared spectroscopy, leucine, Low concentrations, lysine, Methods, Missile bases, Monolayers, nanomaterial, Nanostructured surfaces, Nanostructures, Peptide immobilization, Peptides, Photoelectron spectroscopy, Polysaccharides, Protein adsorption, protein binding, Renal Dialysis, Self assembled monolayers, Self-assembled monolayers, Sulphate, Surface activations, Surface functionalization, Surface Properties, surface property, tetraethylene glycol, unclassified drug, X ray photoelectron spectroscopy, X-ray photoelectron spectroscopies},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-57449118932\&partnerID=40\&md5=8f442ccbde0273f4ed4e04024a75fd37},
	author = {Martins, M.C.L. and Curtin, S.A. and Freitas, S.C. and Salgueiro, P. and Ratner, B.D. and Barbosa, M.A.}
}
@conference {2292,
	title = {A new method for the detection of singular points in fingerprint images},
	booktitle = {2009 Workshop on Applications of Computer Vision, WACV 2009Workshop Appl. Comput. Vis., WACV},
	series = {2009 Workshop on Applications of Computer Vision, WACV 2009},
	year = {2009},
	note = {Conference code: 79821Export Date: 21 November 2012Source: ScopusArt. No.: 5403106doi: 10.1109/WACV.2009.5403106Language of Original Document: EnglishCorrespondence Address: Magalh{\~a}es, F.; INESC, Porto, Portugal; email: filipe.magalhaes@inescporto.ptReferences: Drets, G.A., Liljenstr{\"o}m, H.G., (1999) Intelligent Biometric Techniques in Fingerprint and Face Recognition, , CRC Press, New York;Hong, L., Wan, Y., Fingerprint image enhancement: Algorithm and performance evaluation (1998) IEEE Transactions On, Pattern Analysis and Machine Intelligence, 20 (8), pp. 777-789; 
Jain, A., Hong, L., Bolle, R., On-line fingerprint verification (1997) IEEE Transactions on Pattern Analysis and Machine Intelligence, 19 (4), pp. 302-314; 
Jinwei, G., Jie, Z., A novel model for orientation field of fingerprints (2003) Proceedings IEEE Computer Society Conference on Computer Vision and Pattern Recognition, 2, pp. II493-II498; 
Kara, K., Jain, A.K., Fingerprint classification (1996) Pattern Recognition, 29 (3), pp. 389-404; 
Kass, M., Witkin, A., Analyzing oriented patterns (1987) Cornput. Vision Graph. Image Process, 37 (3), pp. 362-385. , 27996; 
Kawagoe, M., Tojo, A., Fingerprint pattern classification (1984) Pattern Recognition, 17 (3), pp. 295-303; 
Maio, D., Maltoni, D., Cappelli, R., Wayman, J.L., Jain, A.K., FVC2002: Second fingerprint verification competition (2002) Proceedings - International Conference on Pattern Recognition, 16 (3), pp. 811-814; 
Maio, D., Maltoni, D., Cappelli, R., Wayman, J.L., Jain, A.K., Fvc2004: Third fingerprint verification competition (2004) Proceedings International Conference on Biometric Authentication (ICBA), pp. 1-7. , Hong Kong; 
Pankanti, S., Prabhakar, S., Jain, A.K., On the individuality of fingerprints (2002) IEEE Transactions on Pattern Analysis and Machine Intelligence, 24 (8), pp. 1010-1025; 
Sherlock, B.G., Monro, D.M., A model for interpreting fingerprint topology (1993) Pattern Recognition, 26 (7), pp. 1047-1055; 
Srinivasan, V.S., Murthy, N.N., Detection of singular points in fingerprint images (1992) Pattern Recognition, 25 (2), pp. 139-153; 
Zhou, J., Chen, F., Gu, J., A novel algorithm for detecting singular points from fingerprint images (2009) IEEE Transactions on Pattern Analysis and Machine Intelligence, 31 (7), pp. 1239-1250
},
	month = {2009///},
	address = {Snowbird, UT},
	abstract = {Automatic biometric identification based on fingerprints is still one of the most reliable identification method in criminal and forensic applications. A critical step in fingerprint analysis without human intervention is to automatically and reliably extract singular points from the input fingerprint images. These singular points (cores and deltas) not only represent the characteristics of local ridge patterns but also determine the topological structure (i.e., fingerprint type) and largely influence the orientation field. Poincar{\'e} Index-based methods are one of the most common for singular points detection. However, these methods usually result in many spurious detections. Therefore, we propose an enhanced version of the method presented by Zhou et al. [13] that introduced a feature called DORIC to improve the detection. Our principal contribution lies in the adoption of a smoothed orientation field and in the formulation of a new algorithm to analyze the DORIC feature. Experimental results show that the proposed algorithm is accurate and robust, giving better results than the best reported results so far, with improvements in the range of 5\% to 7\%. {\textcopyright} 2009 IEEE.},
	keywords = {Algorithms, Biometric identifications, Biometrics, Computer applications, Computer vision, Critical steps, Fingerprint analysis, Fingerprint images, Forensic applications, Human intervention, Identification method, Orientation fields, Poincare, Ridge patterns, Singular points, Structural optimization, Technical presentations, Topological structure},
	isbn = {9781424454976 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77951158127\&partnerID=40\&md5=718a6649dbc13fcb895f23ffff1363d4},
	author = {Magalh{\~a}es, F. and Oliveira, H.P. and Campilho, A.C.}
}
@article {2308,
	title = {Physical characterization of hydroxyapatite porous scaffolds for tissue engineering},
	journal = {Materials Science and Engineering CMater. Sci. Eng. C},
	volume = {29},
	year = {2009},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.msec.2008.09.052Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: smsilva@ineb.up.ptReferences: Jones, A.C., Milthorpe, B., Averdunk, H., Limaye, A., Senden, T.J., Sakellariou, A., (2004) Biomaterials, 25 (20), p. 4947;Karageorgiou, V., Kaplan, D., (2005) Biomaterials, 26 (27), p. 5474; 
Sepulveda, P., Ortega, F.S., Innocentini, M.D.M., Pandolfelli, V.C., (2000) Journal of the American Ceramic Society, 83 (12), p. 3021; 
Woodard, J.R., Hilldore, A.J., Lan, S.K., Park, C.J., Morgan, A.W., Eurell, J.A., (2007) Biomaterials, 28 (1), p. 45; 
Harris, L.D., Kim, B.S., Mooney, D.J., (1998) Journal of Biomedical Materials Research, 42 (3), p. 396; 
Cyster, L.A., Grant, D.M., Howdle, S.M., Rose, F.R., Irvine, D.J., Freeman, D., (2005) Biomaterials, 26 (7), p. 697; 
Fu, Q., Rahaman, M.N., Bal, B.S., Huang, W., Day, D.E., (2007) Journal of Biomedical Materials Research A, 82 (1), p. 222; 
Chang, B.S., Lee, C.K., Hong, K.S., Youn, H.J., Ryu, H.S., Chung, S.S., (2000) Biomaterials, 21 (12), p. 1291; 
Chu, T.M., Orton, D.G., Hollister, S.J., Feinberg, S.E., Halloran, J.W., (2002) Biomaterials, 23 (5), p. 1283; 
Fu, Q., Rahaman, M.N., Dogan, F., Bal, B.S., (2007) Journal of Biomedical Materials Research B, Applied Biomaterials; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2004) Advanced Materials Forum Ii, 455-456, p. 358; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2004) Bioceramics, 254-2, p. 997. , (16); 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., (2008) Journal of Materials Science-Materials in Medicine, 19 (2), p. 855; 
de Groot, K., (1980) Biomaterials, 1 (1), p. 47; 
Heise, U., Osborn, J.F., Duwe, F., (1990) International Orthopaedics, 14 (3), p. 329; 
Kuboki, Y., Takita, H., Kobayashi, D., Tsuruga, E., Inoue, M., Murata, M., (1998) Journal of Biomedical Materials Research, 39 (2), p. 190; 
Ramay, H.R., Zhang, M., (2003) Biomaterials, 24 (19), p. 3293; 
Kim, H.W., Knowles, J.C., Kim, H.E., (2005) Journal of Materials Science, 16 (3), p. 189; 
Iwano, T., Kurosawa, H., Murase, K., Takeuchi, H., Ohkubo, Y., (1991) Clinical Orthopaedics and Related Research, 268, p. 243; 
Tieliewuhan, Y., Hirata, I., Sasaki, A., Minagi, H., Okazaki, M., (2004) Dental Materials Journal, 23 (3), p. 258; 
Yunoki, S., Ikoma, T., Tsuchiya, A., Monkawa, A., Ohta, K., Sotome, S., (2007) Journal of Biomedical Materials Research B, Applied Biomaterials, 80 (1), p. 166; 
Zhang, Q.Q., Ren, L., Wang, C., Liu, L.R., Wen, X.J., Liu, Y.H., (1996) Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 24 (6), p. 693; 
Zhang, S.M., Cui, F.Z., Liao, S.S., Zhu, Y., Han, L., (2003) Journal of Materials Science, 14 (7), p. 641; 
Yunoki, S., Ikoma, T., Monkawa, A., Marukawa, E., Sotome, S., Shinomiya, K., (2007) Journal of Biomaterials Science, 18 (4), p. 393; 
Teixeira, S., Oliveira, S., Ferraz, M.P., Monteiro, F.J., (2008) Key Engineering Materials, 361-363 (II), p. 947; 
Gibson, I.R., Best, S.M., Bonfield, W., (1999) Journal of Biomedical Materials Research, 44 (4), p. 422; 
Kamitakahara, M., Ohtsuki, C., Kozaka, Y., Ogata, S., Tanihara, M., Miyazaki, T., (2006) Journal of the Ceramic Society of Japan, 114 (1325), p. 82; 
Kim, H.W., Deng, Y., Miranda, P., Pajares, A., Kim, D.K., Kim, H.E., (2001) Effect of Flaw State on the Strength of Brittle Coatings on Soft Substrates, p. 2377
},
	month = {2009///},
	pages = {1510 - 1514},
	abstract = {The present study refers to the preparation and characterization of porous hydroxyapatite scaffolds to be used as matrices for bone regeneration or as specific release vehicles. Ceramics are widely used for bone tissue engineering purposes and in this study, hydroxyapatite porous scaffolds were produced using the polymer replication method. Polyurethane sponges were used as templates and impregnated with a ceramic slurry at different ratios, and sintered at 1300~{\textdegree}C following a specific thermal cycle. The characteristics of the hydroxyapatite porous scaffolds and respective powder used as starting material, were investigated by using scanning electron microscopy, particle size distribution, X-ray diffraction, Fourier transformed infrared spectroscopy and compressive mechanical testing techniques. It was possible to produce highly porous hydroxyapatite scaffolds presenting micro and macropores and pore interconnectivity. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Apatite, Bone, Bone regenerations, Bone tissue engineerings, Ceramic materials, Ceramic slurries, Fourier-transformed infrared spectroscopies, hydroxyapatite, infrared spectroscopy, Macro-pores, Mechanical testing, Physical characterizations, Polymer blends, Polymer replications, Polymers, Polyurethane sponges, Pore interconnectivity, Porous hydroxyapatites, Porous scaffolds, Scaffolds, Scanning electron microscopy, Spectroscopic analysis, Starting materials, Thermal cycles, Tissue engineering, X- ray diffractions},
	isbn = {09284931 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67349181295\&partnerID=40\&md5=ea36fd01a8565ecbd7230948b317995d},
	author = {Teixeira, S. and Rodriguez, M.A. and Pena, P. and De Aza, A.H. and De Aza, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2298,
	title = {Protein adsorption and clotting time of pHEMA hydrogels modified with C18 ligands to adsorb albumin selectively and reversibly},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2009.06.025PubMed ID: 19595453Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, I.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: icastro@ineb.up.ptChemicals/CAS: fibrinogen, 9001-32-5; human serum albumin, 9048-49-1; polymacon, 25053-81-0, 25249-16-5, 98932-78-6; polystyrene, 9003-53-6; Biocompatible Materials; Coated Materials, Biocompatible; Hydrogels; Polyhydroxyethyl Methacrylate, 25249-16-5; Serum AlbuminReferences: Kottkemarchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., Effect of albumin coating on the in vitro blood compatibility of dacron arterial prostheses (1989) Biomaterials, 10 (3), pp. 147-155;Ji, J., Feng, L.X., Barbosa, M.A., Stearyl poly(ethylene oxide) grafted surfaces for preferential adsorption of albumin (2001) Biomaterials, 22 (22), pp. 3015-3023; 
Keogh, J.R., Eaton, J.W., Albumin-binding surfaces for biomaterials (1994) The Journal of Laboratory and Clinical Medicine, 124 (4), pp. 537-545; 
Keogh, J.R., Velander, F.F., Eaton, J.W., Albumin-binding surfaces for implantable devices (1992) Journal of Biomedical Materials Research, 26 (4), pp. 441-456; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces (2003) Journal of Biomaterials Science Polymer Edition, 14 (5), pp. 439-455; 
McFarland, C.D., De Filippis, C., Jenkins, M., Tunstell, A., Rhodes, N.P., Williams, D.F., Albumin-binding surfaces: in vitro activity (1998) Journal of Biomaterials Science Polymer Edition, 9 (11), pp. 1227-1239; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., Alkyl substituted polymers with enhanced albumin affinity (1981) Transactions American Society for Artificial Internal Organs, 27, pp. 499-503; 
Tsai, C.C., Huo, H.H., Kulkarni, P., Eberhart, R.C., Biocompatible coatings with high albumin affinity (1990) ASAIO Transactions, 36 (3), pp. 307-310; 
Wang, D.A., Chen, B.L., Ji, J., Feng, L.X., Selective adsorption of serum albumin on biomedical polyurethanes modified by a poly(ethylene oxide) coupling-polymer with Cibacron blue (F3G-A) endgroups (2002) Bioconjugate Chemistry, 13 (4), pp. 792-803; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) Journal of Biomedical Materials Research Part A, 89 A (3), pp. 642-653; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) Journal of the American Chemical Society, 111 (1), pp. 321-335; 
Kwok, S.C., Mourad, P.D., Crum, L.A., Ratner, B.D., Surface modification of polymers with self-assembled molecular structures: multitechnique surface characterization (2000) Biomacromolecules, 1, pp. 139-148; 
Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-terminated self-assembled monolayers (2005) Biomaterials, 26 (18), pp. 3891-3899; 
Ratner, B.D., Hoffman, A.F., Schoen, F.J., Lemons, J.L., (2004) Biomaterials science - an introduction to materials in medicine. 2nd ed., , Elsevier Academic Press, San Diego, CA, USA; 
Ratner, B.D., Miller, I.F., Interaction of urea with poly(2-hydroxyethyl methacrylate) hydrogels (1972) Journal of Polymer Science Part a-1-Polymer Chemistry, 10 (8), pp. 2425-2445; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of biocompatibility testing, pp. 183-214. , Williams D.F. (Ed), Harcover: CRC Press; 
Cao, L., Chang, M., Lee, C.Y., Castner, D.G., Sukavaneshvar, S., Ratner, B.D., Plasma-deposited tetraglyme surfaces greatly reduce total blood protein adsorption, contact activation, platelet adhesion, platelet procoagulant activity, and in vitro thrombus deposition (2007) Journal of Biomedical Materials Research Part A, 81 (4), pp. 827-837; 
Stovbun, E.V., Kuzaev, A.I., Baturin, S.M., (1996) Polymer Science Series A, 38, pp. 691-695; 
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Yuu, K., Shiroya, T., Blood compatible aspects of poly(2-methoxyethylacrylate) (PMEA) - relationship between protein adsorption and platelet adhesion on PMEA surface (2000) Biomaterials, 21 (14), pp. 1471-1481; 
Castner, D.G., Ratner, B.D., Hirao, A., Nakahama, S., Characterization of poly(2-hydroxyethyl methacrylate) (PHEMA) by XPS (1996) Surface Science Spectra, 4 (1-4), p. 14; 
L{\'o}pez, G.P., Ratner, B.D., Rapoza, R.J., Horbett, T.A., Plasma deposition of ultrathin films of poly(2-hydroxyethyl methacrylate): surface analysis and protein adsorption measurements (1993) Macromolecules, 26 (13), pp. 3247-3253; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., Albumin and fibrinogen adsorption on PU-PHEMA surfaces (2003) Biomaterials, 24 (12), pp. 2067-2076; 
Tsukagoshi, T., Kondo, Y., Yoshino, N., Protein adsorption on polymer-modified silica particle surface (2007) Colloids and Surfaces B Biointerfaces, 54 (1), pp. 101-107; 
Ratner, B.D., Biomedical applications of hydrogels: review and critical appraisal (1981) Biocompatibility of clinical implant materials, , Williams D.F. (Ed), CRC Press; 
H{\"o}{\"o}k, F., V{\"o}r{\"o}s, J., Rodahl, M., Kurrat, R., B{\"o}ni, P., Ramsden, J.J., A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation (2002) Colloids and Surfaces B Biointerfaces, 24 (2), pp. 155-170; 
Stryer, L., (1998) Biochemistry. 4th ed., , WH Freeman, New York; 
Garrett, Q., Chatelier, R.C., Griesser, H.J., Milthorpe, B.K., Effect of charged groups on the adsorption and penetration of proteins onto and into carboxymethylated poly(HEMA) hydrogels (1998) Biomaterials, 19 (23), pp. 2175-2186; 
Mequanint, K., Sheardown, H., 2-Methacryloyloxyethyl N-butylcarbamate: a new co-monomer for synthesis of polyurethane hydrogels with improved mechanical properties for biomedical applications (2005) Journal of Biomaterials Science Polymer Edition, 16 (10), pp. 1303-1318; 
Horbett, T.A., Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. II. Compositional analysis with the prelabeled protein technique (1981) Journal of Biomedical Materials Research, 15 (5), pp. 673-695; 
Feng, L., Andrade, J.D., Protein adsorption on low-temperature isotropic carbon. 3. isotherms, competitivity, desorption and exchange of human albumin and fibrinogen (1994) Biomaterials, 15 (5), pp. 323-333; 
Pitt, W.G., Cooper, S.L., Albumin adsorption on alkyl chain derivatized polyurethanes. 1. The effect of C-18 alkylation (1988) Journal of Biomedical Materials Research, 22 (5), pp. 359-382; 
Markiewski, M.M., Nilsson, B., Ekdahl, K.N., Mollnes, T.E., Lambris, J.D., Complement and coagulation: strangers or partners in crime? (2007) Trends in Immunology, 28 (4), pp. 184-192; 
Zhang, Z., Zhang, M., Chen, S., Horbett, T.A., Ratner, B.D., Jiang, S., Blood compatibility of surfaces with superlow protein adsorption (2008) Biomaterials, 29 (32), pp. 4285-4291; 
Grunkemeier, J.M., Tsai, W.B., Horbett, T.A., Hemocompatibility of treated polystyrene substrates: contact activation, platelet adhesion, and procoagulant activity of adherent platelets (1998) Journal of Biomedical Materials Research, 41 (4), pp. 657-670; 
Gorbet, M.B., Sefton, M.V., Biomaterial-associated thrombosis: roles of coagulation factors, complement, platelets and leukocytes (2004) Biomaterials, 25 (26), pp. 5681-5703
},
	month = {2009///},
	pages = {5541 - 5551},
	abstract = {This work intended to create a nanostructured biomaterial that would bind albumin in a selective and reversible way in order to inhibit the adsorption of other blood proteins and therefore minimize activation of coagulation. Different levels of C18 ligand have been immobilized on poly(2-hydroxyethyl methacrylate) (pHEMA). We hypothesize that samples with intermediate amounts of C18 ligand would allow albumin to recognize them and bind through its hydrophobic pockets specific for long chain fatty acids. Surface characterization has confirmed increasing amounts of C18 ligand on pHEMA as the percentage of C18 in solution increases, with maximum coverage achieved in 10\% C18 samples. Adsorption from pure albumin solution revealed a small decrease in albumin adsorption from pHEMA to 1\% C18 and 2.5\% C18 samples, but on surfaces with 5\% or higher C18 the amount of adsorbed albumin increased as the percentage of C18 increased. Competitive adsorption studies in the presence of both albumin and fibrinogen, and in the presence of all plasma proteins showed that 1\% C18 and 2.5\% C18 were the only surfaces selective for albumin, and that the presence of all plasma proteins may even potentiate albumin adsorption. Reversibility studies demonstrated that both 2.5\% C18 and 5\% C18 samples exchange                        125I-albumin selectively in the presence of both unlabeled albumin and plasma, but 2.5\% C18 samples presented higher exchangeability rates (58\%). Clotting times using recalcified plasma revealed that samples with none or small amounts of C18 (pHEMA to 5\% C18) did not shorten the clotting time compared to the negative control (polystyrene), indicating low activation of the intrinsic coagulation cascade. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.
},
	keywords = {Adsorption, article, Biocompatible Materials, Biomaterials, blood clotting time, Blood Coagulation Tests, Body fluids, Clotting time, Coagulation, Coated Materials, Biocompatible, concentration response, controlled study, Fatty acids, fibrinogen, human serum albumin, hydrogel, Hydrogels, hydrophilicity, Ligands, long chain fatty acid, materials testing, Plasmas, PolyHEMA, Polyhydroxyethyl Methacrylate, polymacon, Polymer blends, polystyrene, Polystyrenes, priority journal, Protein adsorption, protein binding, Proteins, Serum Albumin, Surface modification, surface property, Surface treatment},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68549112719\&partnerID=40\&md5=aa1dd37881210211ef398092a3be6174},
	author = {Gon{\c c}alves, I.C. and Martins, M.C.L. and Barbosa, M.A. and Ratner, B.D.}
}
@article {2309,
	title = {Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {89},
	year = {2009},
	note = {Cited By (since 1996): 11Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32011PubMed ID: 18442116Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, I. C.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Run do Campo Alegre, 823, 4150-180 Porto, Portugal; email: icastro@ineb.up.ptChemicals/CAS: fibrinogen, 9001-32-5; glutaraldehyde, 111-30-8, 37245-61-7; human serum albumin, 9048-49-1; Carbon, 7440-44-0; Ethylene Glycol, 107-21-1; Ligands; Serum AlbuminReferences: Nath, N., Hyun, J., Ma, H., Chilkoti, A., Surface engineering strategies for control of protein and cell interactions (2004) Surf Sci, 570, pp. 98-110;Mao, C., Qiu, Y.Z., Sang, H.B., Mei, H., Zhu, A.P., Shen, J., Lin, S.C., Various approaches to modify biomaterial surfaces for improving hemocompatibility (2004) Adv Colloid Interface Sci, 110, pp. 5-17; 
Gorbet, M.B., Sefton, M.V., Biomaterial-associated thrombosis: Roles of coagulation factors, complement, platelets and leukocytes (2004) Biomaterials, 25, pp. 5681-5703; 
Kottkemarchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., Effect of albumin coating on the in vitro blood compatibility of dacron arterial prostheses (1989) Biomaterials, 10, pp. 147-155; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., Alkyl substituted polymers with enhanced albumin affinity (1981) Trans Am Soc Artif Intern Organs, 27, pp. 499-503; 
Eberhart, R.C., Munro, M.S., Williams, G.B., Kulkarni, P.V., Shannon, W.A., Brink, B.E., Fry, W.J., Albumin adsorption and retention on C-18-alkyl-derivatized polyurethane vascular grafts (1987) Artif Organs, 11, pp. 375-382; 
Frautschi, J.R., Munro, M.S., Lloyd, D.R., Eberhart, R.C., Alkyl derivatized cellulose-acetate membranes with enhanced albumin affinity (1983) Trans Am Soc Artif Intern Organs, 29, pp. 242-244; 
Pitt WG, Cooper SL. Albumin adsorption on alkyl chain derivatized polyurethanes. I. The effect of C-18 alkylation. T Biomed Mater Res 1988;22:359-382Johnston, E., Ratner, B.D., Protein Adsorption: Friend or Foe? (1998) Immobilized biomolecules in analysis: A practical approach, pp. 79-94. , Cass T, Ligler FS, editors, Oxford: Oxford University Press;; 
Ji, J., Feng, L.X., Barbosa, M.A., Stearyl poly(ethylene oxide) grafted surfaces for preferential adsorption of albumin (2001) Biomaterials, 22, pp. 3015-3023; 
Martins, M.C.L., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on cibacron blue F3G-A immobilized onto oligo (ethylene glycol)-terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14, pp. 945-954; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) J Am Chem Soc, 120, pp. 3464-3473; 
Palegrosdemange, C., Simon, E.S., Prime, K.L., Whitesides, G.M., Formation of self-assembled monolayers by chemisorption of derivatives of oligo(ethylene glycol) of structure Hs(Ch2)11 (Och2ch2)meta-Oh on gold (1991) J Am Chem Soc, 113, pp. 12-20; 
Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-ter- minated self-assembled monolayers (2005) Biomaterials, 26, pp. 3891-3899; 
Martins MCL, Ratner BD, Barbosa MA. Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolavers. I Biomed Mater Res 2003;67A: 158-171Owens, D.K., Wendt, R.C., Estimation of surface free energy of polymers (1969) J Appl Polym Sci, 13, pp. 1741-1747; 
Sousa, S.R., Moradas Ferreira, P., Saramago, B., Viseu Melo, L., Barbosa, M.A., Human serum albumin adsorption on TiO 2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Sastry, M., A note on the use of ellipsometry for studying the kinetics of formation of self-assembled monolayers (2000) Bull Mater Sci, 23, pp. 159-163; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ralner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Frank, R.D., Dresbach, H., Thelen, H., Sieberth, H.G., Glutardialde- hyde induced fluorescence technique (GIFT): A new method for the imaging of platelet adhesion on biomaterials (2000) J Biomed Mater Res, 52, pp. 374-381; 
Herrwerth, S., Eck, W., Reinhardt, S., Grunze, M., Factors that determine the protein resistance of oligoether self-assembled monolayers-internal hydrophilicity, terminal hydrophilicity, and lateral packing density (2003) J Am Chem Soc, 125, pp. 9359-9366; 
Harder, P., Grunze, M., Dahint, R., Whitesides, G.M., Laibinis, P.E., Molecular conformation in oligo(ethylene glycol)-terminated self-assembled monolayers on gold and silver surfaces deter-mines their ability to resist protein adsorption (1998) J Phys Chem B, 102, pp. 426-436; 
Schwendel, D., Dahint, R., Herrwerth, S., Schloerholz, M., Eck, W., Grunze, M., Temperature dependence of the protein resistance of poly- and oligo(ethylene glycol)-terminated alkanethiolate monolayers (2001) Langmuir, 17, pp. 5717-5720; 
Zhu, B., Eurell, T., Gunawan, R., Leckband, D., Chain-length dependence of the protein and cell resistance of oligo(ethylene glycol)-terminated self-assembled monolayers on gold (2001) J Biomed Mater Res, 56, pp. 406-416; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (1999) J Biomed Mater Res, 49, pp. 435-447; 
Sperling, C., Schweiss, R.B., Streller, U., Werner, C., In vitro hemocompatibility of self-assembled monolayers displaying various functional groups (2005) Biomaterials, 26, pp. 6547-6557; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethio- lates on gold (2004) Biomaterials, 25, pp. 2557-2563
},
	month = {2009///},
	pages = {642 - 653},
	abstract = {This study focuses on the selective binding of albumin to a nanostructured surfaces to inhibit other blood proteins from adsorbing thereby reducing platelet adhesion and activation. Tetra (ethylene-glycol)-terminated self- assembled monolayers (EG4 SAMs) with different percentages of CI 8 ligands on the surface were characterized by contact angle measurements, X-ray photoelectron microscopy, infrared reflection-absorption spectroscopy, and ellipsometry. A specific surface (2.5\% C18 SAM) was found to be selective for human serum albumin (HSA) in the presence of both albumin and fibrinogen (HFG). The importance of this concentration of C18 ligands was stressed in reversibility studies since that surface exchanged almost all the preadsorbed HSA by HSA in solution, but not by HFG. The effect of protein adsorption in the subsequent adhesion and activation of platelets was studied by pre-immersing the surfaces in albumin and plasma before contact with platelets. Scanning electron microscopy and glutaraldehyde induced fluorescence technique images showed that as surfaces got more hydrophobic due to the immobilization of C18 ligands, the number of adherent platelets increased and their morphology changed from round to fully spread. Pre-immersion in HSA led to an 80{\textdegree}/o decrease in platelet adhesion and reduction of activation. Pre-immersion in 1\% plasma was only relevant in 2.5\% CI 8 SAMs since this was the only surface that demonstrated less adhesion of platelets comparing with buffer pre-immersion. However, they still adsorb more platelets then when HSA was preadsorbed. This was confirmed in competition studies between HSA and plasma that suggested that other plasma proteins were also adsorbing to this surface. {\textcopyright} 2008 Wiley Periodicals, Inc.},
	keywords = {Absorption spectroscopy, Acoustic microscopes, Adhesion, Adsorption, Albumin, Aldehydes, Angle measurement, article, Biological materials, Biomaterials, Blood Platelets, Blood protein, Blood substitutes, Carbon, Cell Shape, chemical composition, Concentration of, Contact angle, controlled study, Ethylene, Ethylene Glycol, ethylene glycol derivative, fibrinogen, Fluorescence technique, glutaraldehyde, gold nanoparticle, human, human serum albumin, Human serum albumins, Humans, hydrophobicity, Infrared reflection absorption spectroscopy, infrared spectroscopy, ligand, Ligands, Nanostructured surface, normal human, Oligo(ethylene glycol), Photoelectron spectroscopy, plasma, Plasma diagnostics, Plasma protein, Plasmas, Platelet adhesion, Platelet Adhesiveness, Platelets, Protein adsorption, protein binding, Proteins, Sams, Scanning electron microscopy, Selective binding, Selective protein adsorption, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, Serum Albumin, Specific surface, Spectrophotometry, Infrared, Surface Properties, surface property, thrombocyte activation, thrombocyte adhesion, thrombocyte shape, X ray photoelectron spectroscopy, X-ray photoelectron microscopy},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-66249128976\&partnerID=40\&md5=0be21ad44fe5cf556598975ea1655bed},
	author = {Gon{\c c}alves, I.C. and Martins, M.C.L. and Barbosa, M.A. and Naeemi, E. and Ratner, B.D.}
}
@article {2304,
	title = {Skin adhesive versus subcuticular suture for perineal skin repair after episiotomy - A randomized controlled trial},
	journal = {Acta Obstetricia et Gynecologica ScandinavicaActa Obstet. Gynecol. Scand.},
	volume = {88},
	year = {2009},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: AOGSAdoi: 10.1080/00016340902883133Language of Original Document: EnglishCorrespondence Address: Mota, R.; Servi{\c c}o de Ginecologia e Obstetr{\'\i}cia, Hospital de S. Jo{\~a}o, 4200-319 Porto, Portugal; email: sisporto@med.up.ptChemicals/CAS: Tissue Adhesives; cyanoacrylic acid octyl ester, 6701-17-3; polyglactin, 26780-50-7, 34346-01-5Tradenames: Dermabond, EthiconManufacturers: EthiconReferences: Kettle, C., Hills, R.K., Jones, P., Darby, L., Gray, R., Johanson, R., Continuous versus interrupted perineal repair with standard or rapidly absorbed sutures after spontaneous vaginal birth: A randomized controlled trial (2002) Lancet, 359, pp. 2217-2223;Gordon, B., Mackrodt, C., Fern, E., Truesdale, A., Ayers, S., Grant, A., The Ipswich Childbirth Study: 1. A randomized evaluation of two stage postpartum perineal repair leaving the skin unsutured (1998) Br J Obstet Gynaecol, 105, pp. 435-440; 
Mackrodt, C., Gordon, B., Fern, E., Ayers, S., Truesdale, A., Grant, A., The Ipswich Childbirth Study: 2. A randomized comparison of polyglactin 910 with chromic catgut for postpartum perineal repair (1998) Br J Obstet Gynaecol, 105, pp. 441-445; 
Kettle, C., Johanson, R.B., Continuous versus interrupted sutures for perineal repair (2000) Cochrane DataBase Syst Rev, (2). , CD000947; 
Kettle, C., Hills, R.K., Ismail, K.M.K., Continuous versus interrupted sutures for repair of episiotomy or second degree tears (2007) Cochrane DataBase Syst Rev, (4). , CD000947; 
Gemynthe, A., Langhoff-Roos, J., Sahl, S., Knudsen, J., New VICRYL formulation: An improved method of perineal repair? (1996) Br J Midwifery, 4, pp. 230-234; 
Oboro, V.O., Tabowei, T.O., Loto, O.M., Bosah, J.O., A multicentre evaluation of the two-layered repair of postpartum perineal trauma (2003) J Obstet Gynaecol, 23, pp. 5-8; 
Kindberg, S., Stehouwer, M., Hvidman, L., Henriksen, T.B., Postpartum perineal repair performed by midwives: A randomised trial comparing two suture techniques leaving the skin unsutured (2008) Br J Obstet Gynaecol, 115, pp. 472-479; 
Gennari, R., Rotmensz, N., Ballardini, B., Scevola, S., Perego, E., Zanini, V., A prospective, randomized, controlled clinical trial of tissue adhesive (2-octylcyanoacrylate) versus standard wound closure in breast surgery (2004) Surgery, 136, pp. 593-599; 
Bernard, L., Doyle, J., Friedlander, S.F., Eichenfield, L.F., Gibbs, N.F., Cunningham, B.B., A prospective comparison of octylcya-noacrylate tissue adhesive (dermabond) and suture for the closure of excisional wounds in children and adolescents (2001) Arch Dermatol, 137, pp. 1177-1180; 
Matin, S.F., Prospective randomized trial of skin adhesive versus sutures for closure of 217 laparoscopic port-site incisions (2003) J Am Coll Surg, 196, pp. 845-853; 
Bhende, S., Rothenburger, S., Spangler, D.J., Dito, M., In vitro assessment of microbial barrier properties of Dermabond( topical skin adhesive (2002) Surg Infect, 3, pp. 251-257; 
Rogerson, L., Mason, G.C., Roberts, A.C., Preliminary experience with twenty perineal repairs using Indermil tissue adhesive (2000) Eur J Obstet Gynecol Reprod Biol, 88, pp. 139-142; 
Bowen, M.L., Selinger, M., Episiotomy closure comparing enbucrilate tissue adhesive with conventional sutures (2002) Int J Gynecol Obstet, 78, pp. 201-205; 
Adoni, A., Anteby, E., The use of Histoacryl for the episiotomy repair (1991) Br J Obstet Gynaecol, 98, pp. 476-478; 
Man, J., Quinn, J., Cyanoacrylate tissue adhesives (1997) Am J Cosm Surg, 14, pp. 413-416; 
Maw, J.L., Quinn, J.V., Wells, G.A., Ducic, Y., Odell, P.F., Lamothe, A., A prospective comparison of octylcyanoacrylate tissue adhesive and suture for the closure of head and neck incisions (1997) J Otolaryngol, 26, pp. 26-30
},
	month = {2009///},
	pages = {660 - 666},
	abstract = {Objective. Surgical repair of perineal lesions after delivery is frequently associated with pain and discomfort, interfering with the normal activities of the puerperium. The aim of this study was to compare perineal skin repair after episiotomy with adhesive glue versus a subcuticular suture, regarding the incidence of pain and wound complications. Study design. Randomized clinical trial. Setting. Tertiary care university hospital. Material and methods. One hundred women having mediolateral episiotomy at vaginal delivery were enrolled. They were randomized to receive skin adhesive (n = 53) or subcuticular suture (n = 47) for closure of perineal skin. The main outcome measure was self-evaluated pain in the 30 days following delivery. Secondary outcome measures were technical difficulties reported with the procedure, duration of surgical repair, wound complications observed at 42-68 hours post-partum and re-initiation of sexual activity by 30 days post-partum. Results. No significant differences were observed between the two groups in incidence of technical difficulties and failed procedures, pain during the procedure, wound complications at hospital discharge, self-evaluated measures of pain at 7 and 30 days or re-initiation of sexual activity by 30 days post-partum. The skin adhesive group had a significantly shorter mean duration of the procedure (four minutes less). Conclusion. Perineal skin closure using adhesive glue is faster than subcuticular suture, and associated with a similar incidence of complications and pain in the first 30 days.},
	keywords = {adult, article, clinical trial, comparative study, controlled clinical trial, controlled study, cyanoacrylic acid octyl ester, edema, episiotomy, Episiotomy closure, Female, granuloma, human, Humans, injury, major clinical study, obstetric care, pain, pain assessment, Perineal repair, perineum, perineum injury, polyglactin, priority journal, questionnaire, randomized controlled trial, sexual intercourse, Skin, Skin adhesive, suture, Suture Techniques, suturing method, Tissue adhesive, Tissue Adhesives, treatment outcome, vaginal delivery, wound closure, wound dehiscence, Wounds and Injuries},
	isbn = {00016349 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-66149113694\&partnerID=40\&md5=2a28b4b09b79b1c79c4cf903af92a16c},
	author = {Mota, R. and Costa, F. and Amaral, A. and Oliveira, F. and Santos, C.C. and Ayres-de-Campos, D.}
}
@conference {2338,
	title = {Automatic cell segmentation from confocal microscopy images of the Arabidopsis root},
	booktitle = {2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBIIEEE Int. Symp. Biomed. Imaging: Nano Macro, Proc., ISBI},
	series = {2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI},
	year = {2008},
	note = {Conference code: 73366Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusArt. No.: 4541095doi: 10.1109/ISBI.2008.4541095Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A., Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo (2007) Motion07, pp. 13-13; 
Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of arabidopsis thaliana root meristem in confocal microscopy (2004) Proc. of the Inter. Conf. on Image Analysis and Recognition, pp. 166-174; 
Perona, P., Malik, J., Scale-space and edge detection using anisotropic diffusion (1990) IEEE Trans. on PAMI, 7 (12), pp. 629-639; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095. , August; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations IEEE Trans. on PAMI, 13 (6); 
Meyer, F., Beucher, S., Morphological segmentation (1990) JVCIR, 1 (1), pp. 21-46. , Sept; 
Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Underst, 77 (3), pp. 317-370; 
Kovesi, P., Symmetry and asymmetry from local phase (1997) Proc. of the Tenth Australian Joint Conf. on Artificial Intelligence, , December; 
Burges, C., A tutorial on support vector machines for pattern recognition (1998) Data Mining and Knowledge Discovery, 2 (2), pp. 121-167
Sponsors: Institute of Electrical and Electronic Engineers; Engineering in Medicine and Biology Society; Signal Processing Society},
	month = {2008///},
	pages = {712 - 715},
	address = {Paris},
	abstract = {In vivo observation and tracking of cell division in the Arabidopsis thaliana root meristem, by time-lapse confocal microscopy, is central to biology research. The research herein described is based on large amount of image data, which must be analyzed to determine the location and state of cells. The possibility of automating the process of cell detection/marking is an important step to provide research tools to the biologists in order to ease the search for a special event as cell division. This paper discusses an automatic cell segmentation method, which selects the best cell candidates from a starting watershed based image segmentation. The selection of individual cells is obtained using a Support Vector Machine (SVM) classifier, based on the shape and edge strength of the cells{\textquoteright} contour. The resulting segmentation is largely pruned of badly segmented cells, which can reduce the false positive detection of cell division. This is a good result on its own and a starting point for improvement of cell segmentation methodology. {\textcopyright}2008 IEEE.},
	keywords = {Biological cells, Biomedical image processing, Cell divisions, cell proliferation, Cell segmentation, Cells, confocal microscopy, image processing, Image segmentation, Microscopic examination, Support vector machines, Technical presentations},
	isbn = {9781424420032 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-51049123600\&partnerID=40\&md5=9e4652fa61936d105b4059db95e63f78},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2358,
	title = {Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {19},
	year = {2008},
	note = {Cited By (since 1996): 23Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-007-3005-xPubMed ID: 17665126Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: smsilva@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Ceramics; Durapatite, 1306-06-5References: Jones, A.C., (2004) Biomaterials, 25, p. 4947;Karaggeorgiou, V., (2005) Biomaterials, 27, p. 5474; 
Leon, C.A., Leon, Y., (1998) Adv. Colloid. Interface Sci., 76-77, p. 341; 
Kuboki, Y., Takita, H., Kobayashi, D., Tsuruga, E., Inoue, M., Murata, M., (1998) J. Biomed. Mater. Res., 39, p. 190. , 2; 
Heise, U., Osborn, J.F., Duwe, F., (1990) Int. Orthop., 14, p. 329; 
Bucholz, R.W., Carlton, A., Holmes, R.E., (1987) Orthop. Clin. North. Am., 18, p. 323; 
Hill, P.A., (1998) Br. J. Orthod., 25, p. 101; 
Martin, R.B., (1999) Mater. Sci. Forum, 293, p. 5; 
Nunes, C.R., Simske, S.J., Sachdeva, R., Wolford, L.M., (1997) J. Biomed. Mater. Res., 36, p. 560; 
Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Key Eng. Mater., 254-256, p. 997; 
Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Mater. Sci. Forum, 455-456, p. 358; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Porous, H.A., (2005) Key Eng. Mater., 284-286; 
Teixeira, S.M., Queiroz, A.C., Monteiro, F.J., Ferraz, M.P., In vitro analysis of macroporous ceramic matrices: Preliminary results (2005) 19th European Conference on Biomaterials, , PP-P173; Naples, Italy, Sept 11-15
},
	month = {2008///},
	pages = {855 - 859},
	abstract = {Hydroxyapatite porous scaffolds can be used for tissue engineering applications since they can serve as templates for cell adhesion, proliferation and ultimately for tissue repair. One way to address this issue is to evaluate the cell adhesion using several characterization techniques namely, cytotoxicity assays and cell visualization. On the other hand, when using highly macroporous scaffolds some techniques may not be adequate for evaluation, such as MTT. In this work, cytotoxicity assay (MTS), scanning electron microscopy (SEM) and Confocal laser scanning microscopy (CLSM) were used to evaluate cell adhesion in highly macroporous hydroxyapatite scaffolds. It was possible to observe that some techniques were not suitable to evaluate cell adhesion. In addition, it was shown that for this kind of scaffolds, confocal laser scanning microscopy is a powerful tool for cell adhesion and proliferation evaluation. {\textcopyright} 2007 Springer Science+Business Media, LLC.},
	keywords = {article, Bioassay, Biocompatibility, Biocompatible Materials, cell adhesion, Cell Line, Tumor, cell proliferation, cell structure, Ceramic materials, ceramics, confocal laser microscopy, controlled study, Cytotoxicity, Cytotoxicity assay, Durapatite, human, human cell, Humans, hydroxyapatite, Macroporous ceramic scaffolds, Microscopy, Confocal, Microscopy, Electron, Scanning, Osteoblasts, priority journal, Scanning electron microscopy, Tissue engineering, Tissue Scaffolds},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-39049162092\&partnerID=40\&md5=500dfec2998ea8943e9a38309d2b7e5b},
	author = {Teixeira, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2348,
	title = {Chromatographic pattern classification},
	journal = {IEEE Transactions on Biomedical EngineeringIEEE Trans. Biomed. Eng.},
	volume = {55},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusArt. No.: 8CODEN: IEBEAdoi: 10.1109/TBME.2008.919852PubMed ID: 18714832Language of Original Document: EnglishCorrespondence Address: Sousa, A. V.; Instituto de Engenharia Biom{\'e}dica (INEB), Instituto Superior de Engenharia Do Porto, 4200-465 Porto, Portugal; email: ats@isep.ipp.ptChemicals/CAS: Hydrolases, EC 3.-References: Keir, G., Winchester, B., Clayton, P., Carbohydrate-deficient glycoprotein syndromes: Inborn errors of protein glycosylation (1999) Ann. Clin. Biochem, 36 (1), pp. 20-36;Durand, G., Seta, N., Protein glycosylation and diseases: Blood and urinary oligosaccharides as markers for diagnosis and therapeutic monitoring (2000) Clin. Chem, 46, pp. 795-805; 
Scriver, S., Beaudet, A., Sly, W., Valle, D., (2001) The Metabolic Bases of Inherited Disease, 3. , New-York: McGraw-Hill; 
Pinto, R., Caseiro, C., Lemos, M., Lopes, L., Fontes, A., Miranda, M., Prevalence of lysosomal storage diseases in Portugal (2004) Eur. J. Hum. Genet, 12, pp. 87-92; 
Friedman, R., Williams, M., Moser, H., Kolodny, E., Improved thin-layer chromatographic method in the diagnosis of mannosidosis (1978) Clin. Chem, 24, pp. 1576-1577; 
Kurczynski, T., Kendzierski, K., Sewell, A., Kuczynski, T., Urinary oligosaccharides in pregnant or lactating women: Pitfall in screening (1993) Clin. Chem, 39, pp. 2346-2347; 
Sewell, A., Urinary oligosaccharides (1991) Techniques Diagnostic Human Biochemical Genetics, pp. 219-231. , New York: Wiley; 
Reich, E., Blatter, A., Modern TLC: A key technique for identification and quality control of botanicals and dietary supplements (2005) J. Planar Chromatogr. - Modern TLC, 18, pp. 34-37. , Jan./Feb; 
Duvdevani-Bar, S., Edelman, S., Visual recognition and categorization on the basis of similarities to multiple class prototypes (1999) Int. J. Comput. Vis, 33, pp. 201-228; 
Pekalska, E., Duin, R., Dissimilarity representations allow for building good classifiers (2002) Pattern Recognit. Lett, 23, pp. 943-956; 
Goldstone, R., Son, J., Similarity (2005) Cambridge Handbook of Thinking and Reasoning, pp. 13-36. , K. Holyoak and R. Morrison, Eds. Cambridge, MA: Cambridge Univ. Press; 
Lavine, B., Clustering and classification of analytical data (2000) Encyclopedia of Analytical Chemistry: Instrumentation and Applications, pp. 9689-9710. , R. Meyers, Ed. Chichester, U.K, Wiley; 
Japkowicz, N., Learning from imbalanced data sets: A comparison of various strategies (2000) Proc. Learn. Imbalanced Data, pp. 10-15; 
Weiss, G., Mining with rarity: A unifying framework (2004) Sigkdd Explor, 6, pp. 7-19; 
Kubat, M., Holte, R., Matwin, S., Machine learning for the detection of oil spills in satellite radar images (1998) Mach. Learn, 30, pp. 195-215; 
Tax, D., One-class classification; concept-learning in the absence of counter-examples, (2001), ASCI, pp. 1-190. , Ph.D. dissertation, Delft Univ. Technol, Dissertation Ser, ppSousa, A., Aguiar, R., Mendon{\c c}a, A., Campilho, A., Automatic lane and band detection in images of thin layer chromatography (2004) Lecture Notes in Computer Science, 3212, pp. 158-165. , Image Analysis and Recognition, New York: Springer-Verlag; 
Schwedt, G., (1997) The Essential Guide to Analytical Chemistry, , New York: Wiley; 
Bajla, I., Hollander, I., Fluch, S., Burg, K., Kollar, M., An alternative method for electrophoretic gel image analysis in the GelMaster software (2005) Comput. Methods Programs Biomed, 77, pp. 209-231; 
Eibrand, R., Kennedy, P., Cotter, D., MacEvilly, U., Wu, B., Analysis of Atlantic salmon skin mucus: COPS - A computer-based system for protein pattern analysis of 1D SDS-PAGE gels (2003) Proc. IEEE Symp. Bioinf. BioEng, pp. 45-52; 
Gerasimov, A., Use of the software processing of scanned chromatogram images in quantitative planar chromatography (2004) J. Anal. Chem, 59, pp. 348-353; 
Goulding, P., (2000) Electrophoresis Gel Analysis Software: Important Aspects, p. 14. , Cambridge, U.K, UVItec Ltd; 
Jedra, M., El Khattabi, N., Limouri, M., Essaid, A., Recognition of seed varieties using a time-delay neural network: Analysis of electrophoretic images (1999) Comput. Electron. Agric, 22, pp. 1-10; 
Machado, A., Campos, M., Siqueira, A., Carvalho, O., An iterative algorithm for segmenting lanes in gel electrophoresis images (1997) Proc. Comput. Graph. Image Process. X Braz. Symp, pp. 140-146. , Campos do Jordao, Brazil, Oct; 
Ye, X., Suen, C., Cheriet, M., Wang, E., A recent development in image analysis of electrophoresis gels (1999) Proc. Vis. Interface, pp. 432-438; 
Shadle, S., Allen, D., Guo, H., Pogozelski, W., Bashkin, J., Tullius, T., Quantitative analysis of electrophoresis data: Novel curve fitting methodology and its application to the determination of a protein-DNA binding constant (1997) Nucleic Acids Res, 25, pp. 850-860; 
Bajla, I., Hollander, I., Burg, K., Improvement of electrophoretic gel image analysis (2001) Meas. Sci. Rev, 1, pp. 5-10; 
Bajla, I., Hollander, I., Burg, K., Fluch, S., A novel approach to quantitative analysis of electrophoretic gel images of DNA fragments (2002) Proc. IEEE Int. Symp. Biomed. Imag, pp. 899-902; 
Lonni, A., Scarminio, I., Silva, L., Ferreira, D., Differentiation of species of Baccharis Genus by HPLC and chemometrics methods (2003) Anal. Sci, 19, pp. 1013-1017; 
Beltr{\'a}n, N., Duarte-Mermoud, M., Salah, S., Bustos, M., Feature selection algorithms using Chilean wine chromatograms as examples (2005) J. Food Eng, 67, pp. 483-490; 
Landgrebe, D., Information extraction principles and methods for multispectral and hyperspectral image data (2000) Information Processing for Remote Sensing, , Hackensack, NJ: World Scientific, ch. 1; 
Landgrebe, D., Hyperspectral image data analysis as a high dimensional signal processing problem (2002) IEEE Signal Process. Mag, 19 (1), pp. 17-28. , Jan; 
Jimenez, L., Landgrebe, D., Hyperspectral data analysis and feature reduction via projection pursuit (1999) IEEE Trans. Geosci. Remote Sens, 37 (6), pp. 2653-2667. , Nov; 
Jimenez, L., Landgrebe, D., Supervised classification in high- dimensional space: Geometrical, statistical, and asymptotical properties of multivariate data (1998) IEEE Trans. Syst., Man, Cybern, 28 (1), pp. 39-54. , Pt. C, Feb; 
Marques deS{\'a}, J., (2001) Pattern Recognition - Concepts, Methods and Applications, , Berlin, Germany: Springer-Verlag; 
Heijden, F., Duin, R., Ridder, D., Tax, D., (2004) Classification, Parameter Estimation and State Estimation, , New York: Wiley; 
Dudoit, S., Fridlyand, J., Speed, T., Comparison of discrimination methods for the classification of tumors using gene expression data (2002) J. Amer. Stat. Assoc, 97, pp. 77-87; 
Zomer, S., Brereton, R., Carter, J., Eckers, C., Support vector machines for the discrimination of analytical chemical data: Application to the determination of tablet production by pyrolysis-gas chromatography-mass spectrometry (2004) Analyst, 129, pp. 175-181; 
Zomer, S., Guillo, C., Brereton, R., Hanna-Brown, M., Toxicological classification of urine samples using pattern recognition techniques and capilarity electrophoresis (2003) Anal. Bioanal. Chem, 378, pp. 2008-2020; 
Fan, X., Bai, J., Shen, P., Diagnosis of breast cancer using HPLC metabonomics fingerprints coupled with computational methods (2005) 27th Annu. Conf. Eng. Med. Biol, pp. 6081-6084. , Shanghai, China; 
Skurichina, M., Raudys, S., Duin, R., K-nearest neighbors directed noise injection in multilayer perceptron training (2000) IEEE Trans. Neural Netw, 11 (2), pp. 504-511. , Mar; 
Ha, T., Bunke, H., Off-line, handwritten numeral recognition by perturbation method (1997) IEEE Trans. Pattern Anal. Mach. Intell, 19, pp. 535-539; 
Lee, S., Noisy replication in skewed binary classification (2000) Comput. Stat. Data Anal, 34, pp. 165-191; 
Demant, C., Streicher-Abel, B., Waszkewitz, P., (1999) Industrial Image Processing, , New York: Springer-Verlag; 
Dempster, A., Laird, N., Rubin, D., Maximum likelihood from incomplete data via the EM algorithm (1977) J. Roy. Stat. Soc. B, 39 (1), pp. 1-38; 
Mor{\'e}, J., Sorensen, D., Computing a trust region step (1983) SIAM J. Sci. Stat. Comput, 4, pp. 553-572; 
Byrd, R., Schnabel, R., Shultz, G., Approximate solution of the trust region problem by minimization over two-dimensional subspaces (1988) Math. Program, 40, pp. 247-263; 
Sousa, A., Mendon{\c c}a, A., Campilho, A., Aguiar, R., Miranda, C., Feature extraction for classification of thin-layer chromatography images (2005) Lecture Notes in Computer Science, 3656, pp. 974-981. , Image Analysis and Recognition, New York: Springer-Verlag; 
Ripley, B., (2004) Pattern Recognition and Neural Networks, , New York: Cambridge Univ. Press; 
Visa, S., Ralescu, A., Learning imbalanced and overlapping classes using fuzzy sets (2003) ICML 2003, Workshop Learn. Imbalanced Datasets, 2. , presented at the, Washington, DC
},
	month = {2008///},
	pages = {1687 - 1696},
	abstract = {In this paper, we propose and evaluate methodologies for the classification of images from thin-layer chromatography. Each individual sample is characterized by an intensity profile that is further represented into a feature space. The first steps of this process aim at obtaining a robust estimate of the intensity profile by filtering noise, reducing the influence of background changes, and by fitting a mixture of Gaussians. The resulting profiles are represented by a set of appropriate features trying to characterize the state of nature, here spread out over four classes, one for normal subjects and the other three corresponding to lysosomal diseases, which are disorders responsible for severe nerve degeneration. For classification purposes, a novel solution based on a hierarchical structure is proposed. The main conclusion of this paper is that an automatically generated decision tree presents better results than more conventional solutions, being able to deal with the natural imbalance of the data that, as consequence of the rarity of lysosomal disorders, has very few representative cases in the disease classes when compared with the normal population. {\textcopyright} 2008 IEEE.},
	keywords = {Acoustic variables control, Algorithms, article, Chromatographic analysis, Chromatographic pattern features, Chromatographic profiles, Chromatography, Chromatography, Thin Layer, Classification, Diagnosis, Computer-Assisted, Gaussian noise (electronic), Hierarchical classifier, Humans, Hydrolases, imaging, Lysosomal Storage Diseases, lysosome storage disease, mathematical analysis, nerve degeneration, noise, normal distribution, Pattern recognition, Pattern Recognition, Automated, Reproducibility of Results, sensitivity and specificity, Urinalysis},
	isbn = {00189294 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-44349183146\&partnerID=40\&md5=df677f44d528c936310d8c547624a550},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2364,
	title = {A clinical report of bone regeneration in maxillofacial surgery using bonelike{\textregistered} synthetic bone graft},
	journal = {Journal of Biomaterials ApplicationsJ. Biomater. Appl.},
	volume = {22},
	year = {2008},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JBAPEdoi: 10.1177/0885328207078260Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: Bone SubstitutesReferences: Mohamed, A., Sudha, K., Kim, F., Karl, K., Scott, P.B., Bone Graft Substitutes (2003) Cell Based Approaches for Bone Graft Substitutes, p. 127. , In: Laurencin, C.T. (ed.), Chapter 7 ASTM - International, USA;Mellonig, J.T., Prewett, A.B., Moyer, M.P., HIV Inactivation in a Bone Allograft (1992) J. Periodontol., 63, p. 979; 
Buck, B.E., Malinin, T.I., Brown, M.D., Bone Transplantation and Human Immunodeficiency Virus. An Estimate of Risk of Acquired Immunodeficiency Syndrome (AIDS) (1989) Clin. Orthop., 240, p. 129; 
Mary, E.A.R., Raymond, A.Y., Bone Replacement Grafts - The Bone Substitutes (1998) Dent. Clin. North Am., 42 (3), p. 491; 
Doron, I.I., Amy, L.L., Bone Graft Substitutes (2002) Oper. Tech. Plast. Reconsr. Surg., 9 (4), p. 151; 
Wright, S., Commentary The Bone-Graft Market in Europe, in Emerging Technologies in Orthopedics I: Bone Graft Substitutes (1999) Bone Growth Stimulators and Bone Growth Factors By Datamonitor Plc, p. 591; 
Knaack, D., Goad, M.E.P., Aiolova, M., Christian, R., Ali, T., Promod, C., Duke Lee, D., Resorbable Calcium Phosphate Bone Substitute (1998) J. Biomed. Mater. Res., 43, p. 399; 
Frayssinet, P., Trouillet, J.L., Rouquet, N., Azimus, E., Autefage, A., Osseointegration Macroporous Calcium Phosphate Ceramics Having a Different Chemical Composition (1993) Biomaterials, 14, p. 423; 
Klein, C.P.A.T., Driessen, A.A., Groot de, K., van den Hooff, A., Biodegradation Behavior of Various Calcium Phosphate Materials in Bone Tissue (1983) J. Biomed. Mater. Res., 17, p. 769; 
Daculsi, G., Biphasic Calcium Phosphate Concept Applied to Artificial Bone, Implant Coating and Injectable Bone Substitute (1998) Biomaterials, 19, p. 1473; 
Santos, J.D., Hastings, G.W., Knowles, J.C., Sintered Hydroxyapatite Compositions and Method for the Preparation Thereof (1999) Worldwide Applications, , (PCT), Patent No. 1 189 851; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass Reinforced Hydroxyapatite: A Comprehensive Study of the Effect of Glass Composition on the Crystallography of the Composite (1998) J. Biomed. Mater. Res., 39, p. 244; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass-reinforced Hydroxyapatite Composites: Fracture Toughness and Hardness Dependence on Microstructural Characteristics (1999) Biomaterials, 20, p. 2085; 
Lopes, M.A., Silva, R.F., Monteiro, F.J., Santos, J.D., Microstructural Dependence of Young{\textquoteright}s and Shear Moduli of P 2 O 2 Glass Reinforced Hydroxyapatite for Biomedical Applications (2000) Biomaterials, 21, p. 749; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Push-out Testing and Histological Evaluation of Glass Reinforced Hydroxyapatite Composites Implanted in the Tibia of Rabbits (2001) J. Biomed. Mater. Res., 54, p. 463; 
Lobato, J.V., Hussain, N.S., Botelho, C.M., Rodrigues, J.M., Lu{\'\i}s, A.L., Maur{\'\i}cio, A.C., Lopes, M.A., Santos, J.D., Assessment of the Potential of Bonelike{\textregistered} Graft for Bone Regeneration by using an Animal Model (2005) Key. Eng. Mater., 877, pp. 284-286; 
Lobato, J.V., Hussain, N.S., Botelho, C.M., Mauricio, A.C., Afonso, A., Ali, N., Santos, J.D., Assessment of Bonelike{\textregistered} Graft with a Resorbable Matrix using an Animal Model (2006) Thin Solid Films, 515, p. 362; 
Duarte, F., Santos, J.D., Afonso, A., Medical Applications of Bonelike{\textregistered} in Maxillofacial Surgery (2004) Mater. Sci. Forum, 370, pp. 455-456; 
Gutierres, M., Hussain, N.S., Afonso, A., Almeida, L., Cabral, T., Lopes, M.A., Santos, J.D., Biological Behaviour of Bonelike{\textregistered} Graft Implanted in Tibia of Humans (2005) Key Eng. Mater., 1041, pp. 284-286; 
LeGeros, R.Z., Properties of Osteoconductive Biomaterials: Calcium Phosphates (2002) Clin. Orthop., 395, p. 81; 
Robert, D.A.G., Hanneke, G.T., Ronald, J.H., Buma, P., Mechanism of Bone Incorporation of β-TCP Bone Substitute in Open Wedge Tibial Osteotomy in Patients (2005) Biomaterials, 26, p. 6713; 
Hirotsugu, H., Norio, A., Kimimitsu, O., Noritaka, F., Ohnishi, H., Okada, A., Nomura, S., Maeda, T., A Histological Evaluation on Self setting α-tricalcium Phosphate Applied in the Rat Bone Cavity (2004) Biomaterials, 25, p. 431; 
Meinel, L., Betz, O., Fajardo, R., Hofmann, S., Nazarian, A., Cory, E., Hilbe, M., Kirker-Head, C., Silk Based Biomaterials to Heal Critical Sized Femur Defects (2006) Bone, 39, p. 922; 
Lim, S.C., Lee, M.J., Yeo, H.H., Effects of Various Implant Materials on Regeneration of Calvarial Defects in Rats (2000) Pathol. Int., 50, p. 594; 
Carano, R.A.D., Filvaroff, E.H., Angiogenesis and Bone Repair (2003) Drug Discov. Today, 21, p. 980; 
Gutierres, M., Hussain, N.S., Lopes, M.A., Afonso, A., Cabral, A.T., Almeida, L., Santos, J.D., Histomorphometric Measurements Histological and SEM Analyses of bone/ implant Interface: Clinical Trials using Bonelike{\textregistered} Granules (2006) J. Orthop. Res., 24, p. 953
},
	month = {2008///},
	pages = {373 - 385},
	abstract = {The objective of this study is to evaluate the osteoconductivity and bioactivity of the Bonelike{\textregistered} graft in repairing surgical cystic bone defects. Bonelike{\textregistered} is implanted in 11 patients, aged between 24 and 53 years with a mean age of 36 years, consisting of 5 men and 6 women. According to the standard follow up protocols, radiological examinations are performed and Bonelike{\textregistered} /bone retrieved samples have been analyzed histologically using non-decalcified sections obtained perpendicular to bone length axis. Radiographic examination and histological results clearly demonstrate an extensive new bone formation apposed on Bonelike{\textregistered} granules with a significant degree of maturation. These clinical applications in maxillary bone defects indicate perfect bone bonding between new bone formed and Bonelike{\textregistered} granules, along with partial surface biodegradation. This quick and effective osteoconductive response from Bonelike{\textregistered} may reduce the time needed to reconstruct the bone defected area of patients. {\textcopyright} 2008 Sage Publications.},
	keywords = {adult, article, Bioactivity, Bone, bone prosthesis, Bone Regeneration, Bone Substitutes, bone transplantation, Bonelike{\textregistered}, clinical trial, facial bone, Facial Bones, Female, Histological studies, histology, human, Humans, Implants (surgical), instrumentation, male, Maxillofacial surgery, methodology, Middle Aged, oral surgery, Pathology, Radiography, Radiology, Regeneration, Surgery, Surgery, Oral, Synthetic bone graft, treatment outcome},
	isbn = {08853282 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-37249042186\&partnerID=40\&md5=ab2c9e9614d3f4f521445c149cff57ec},
	author = {Sousa, R.C. and Lobato, J.V. and Maur{\'\i}cio, A.C. and Hussain, N.S. and Botelho, C.M. and Lopes, M.A. and Santos, J.D.}
}
@article {2342,
	title = {Comparative study of nanohydroxyapatite microspheres for medical applications},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {86},
	year = {2008},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31634PubMed ID: 17975824Language of Original Document: EnglishCorrespondence Address: Mateus, A. Y. P.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium hydroxide, 1305-62-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium, 7440-70-2; dodecyl sulfate sodium, 151-21-3; neutral red, 553-24-2; phosphoric acid, 7664-38-2; Biocompatible Materials; Durapatite, 1306-06-5; PowdersReferences: Jarcho, M., Calcium phosphates ceramics as hard tissue prosthetics (1981) Clin Orthop Relat Res, 157, pp. 259-278;Bucholz, R., Carlton, A., Holmes, R., Hydroxyapatite and tricalcium phosphate bone graft substitutes (1987) Orthop Clin N Am, 18, pp. 323-334; 
Daculsi, G., Passuti, N., Martins, S., Deudon, C., LeGeros, R., Raher, S., Macroporous calcium phosphate ceramic for ling bone surgery in humans and dogs: Clinical and histological study (1990) J Biomed Mater Res, 24, pp. 379-396; 
Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone in growth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Yuan, H., Kurashina, K., de Bruijn, J., Li, Y., de Groot, K., Zhang, X., A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics (1999) Biomaterials, 20, pp. 1799-1806; 
Liou, S., Chen, S., Liu, D., Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios (2003) Biomaterials, 24, pp. 3981-3988; 
Earl, J.S., Wood, D.J., Mile, S.J., Hydrothermal synthesis of hydroxyapatite (2006) J Phys Conf, 26, pp. 268-271; 
Han, J.K., Song, H.Y., Fumio, S., Lee, B.T., Synthesis of high purity nano-sized hydroxyapatite powder by microwave-hydrothermal method (2006) Mater Chem Phys, 99, pp. 235-239; 
Yoshimura, M., Sujaridworakun, P., Koh, F., Fujiwara, T., Pongkao, D., Ahniyaz, A., Hydrothermal conversion of calcite crystals to hydroxyapatite (2004) Mater Sci Eng C, 24, pp. 521-525; 
Bogdanoviciene, I., Beganskiene, A., Tonsuaadu, K., Glaser, J., Meyer, H.J., Kareiva, A., Calcium hydroxyapatite Ca 10(PO 4) 6 (OH) 2 ceramics prepared by aqueous sol-gel processing (2006) Mater Res Bull, 41, pp. 1754-1762; 
Liu, D.M., Troczynski, T., Tseng, W.J., Water-based sol-gel synthesis of hydroxyapatite: Process development (2001) Biomaterials, 22, pp. 1721-1730; 
Bezzi, G., Celotti, G., Landi, E., La Torretta, T.M.G., Sopyan, I., Tampieri, A., A novel sol-gel technique for hydroxyapatite preparation (2003) Mater Chem Phys, 78, pp. 816-824; 
An, G.H., Wang, H.J., Kim, B.H., Jeong, Y.G., Choa, Y.H., Fabrication and characterization of a hydroxyapatite nanopowder by ultrasonic spray pyrolysis with salt-assisted decomposition (2007) Mater Sci Eng A, pp. 449-451,821-824; 
Varma, H.K., Babu, S., Synthesis of calcium phosphate bioceramics by citrate gel pyrolisis method (2005) Ceram Int, 31, pp. 109-114; 
Jarudilokkula, S., Tanthapanichakoonb, W., Boonamnuayvittayaa, V., Synthesis of hydroxyapatite nanoparticles using an emulsion liquid membrane system Colloids Surf A, , Forthcoming; 
Bose, S., Saha, S.K., Synthesis and characterization of hydroxyapatite nanopowders by emulsion technique (2003) Chem Mater, 15, pp. 4464-4469; 
Nayar, S., Sihna, M.K., Basu, D., Sinha, A., Synthesis and sintering of biomimetic hydroxyapatite nanoparticles for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 1063-1068; 
Tas, A.C., Synthesis of biomimetic Ca-hydroxyapatite powders at 37{\textdegree}C in synthetic body fluids (2000) Biomaterials, 21, pp. 1429-1438; 
Wanga, A., Yina, H., Liua, D., Wub, H., Wadac, Y., Rena, M., Xua, Y., Chenga, X., Effects of organic modifiers on the size-controlled synthesis of hydroxyapatite nanorods (2007) Appl Surf Sci, 253, pp. 3311-3316; 
Zhang, H.G., Zhu, Q., Surfactant-assisted preparation of fluoride-substituted hydroxyapatite nanorods (2005) Mater Lett, 59, pp. 3054-3058; 
Liu, Y., Houa, D., Wangb, G., A simple wet chemical synthesis and characterization of hydroxyapatite nanorods (2004) Mater Chem Phys, 86, pp. 69-73; 
Pang, Y.X., Bao, X., Influence of temperature, ripening time and calcination on the morphology and crystallinity of hydroxyapatite nanoparticles (2003) J Eur Ceram Soc, 23, pp. 1697-1704; 
Gitzhofer, B.E., Boulos, M.I., Morpholgical study of hydroxyapatite nanocrystal suspension (2000) J Mater Sci: Mater Med, 11, pp. 523-531; 
Barbosa, M., Granja, P., Barrias, C., Amaral, I., Polysaccharides as scaffolds for bone regeneration (2005) ITBM-RBM, 26, pp. 212-217; 
Hari, P., Chandy, T., Sharma, C., Chitosan/calcium-alginate beads for oral delivery of insulin (1996) J Appl Polym Sci, 59, pp. 1796-1801; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Key Eng Mater, pp. 254-262,877-880; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 455-463; 
Bowersock, T., Martins, S., Vaccine delivery to animals (1999) Adv Drug Deliv Rev, 32, pp. 167-194; 
Kong, H., Smith, M., Mooney, D., Designing alginate hydrogels to maintain viability of immobilized cells (2003) Biomaterials, 24, pp. 4023-4029; 
Legallais, C., David, B., Dor{\'e}, E., Bioartificial livers (BAL): Current technological aspects and future developments (2001) J Membr Sci, 181, pp. 81-95; 
Liu, X.D., Yu, W.Y., Zhang, Y., Xue, W.M., Yu, W.T., Xiong, Y., Ma, X.J., Yuan, Q., Characterization of structure and diffusion behaviour of Ca-alginate beads prepared with external or internal calcium sources (2002) J Microencapsul, 19 (6 -1), pp. 775-782; 
Taqieddin, E., Amiji, M., Enzyme immobilization in novel alginate-chitosan coreshell microcapsules (2004) Biomaterials, 25, pp. 1937-1945; 
Ribeiro, C., Barrias, C., Barbosa, M., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Kim, Y.J., Park, H.G., Yang, Y.L., Yoon, Y., Kim, S., Oh, E., Multifunctional drug delivery system using starch-alginate beads for controlled release (2005) Biol Pharm Bull, 28, pp. 394-397; 
Barrias, C., Ribeiro, C., Lamghari, M., Sa, C., Barbosa, M., Proliferation, activity and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) J Biomed Mater Res A, 72, pp. 57-66; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration (2007) Key Eng Mater, pp. 330-332,243-246; 
Mankani, M., Fowler, B., Kingman, A., Robey, P., In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape (2001) Biotechonol Bioeng, 72, pp. 96-107; 
Higashi, T., Okamoto, H., Influence of particle size of calcium phosphate ceramics as a capping agent on the formation of a hard tissue barrier in amputated dental pulp (1996) J Endodoncy, 22, pp. 281-283; 
Guo, G., Xun, Y., Wnag, Z., Guo, H., Preparation of hydroxyapatite nanoparticles by reverse microemulsion (2005) Ceram Int, 331, pp. 869-872; 
Sun, X.M., Chen, X., Deng, Z.X., Li, Y.D., A CTAB-assited hydrothermal orientation growth of ZnO nanorods (2002) Mater Chem Phys, 78, pp. 99-104; 
Uota, M., Arakawa, H., Kitamura, N., Yoshimura, T., Tanaka, J., Kijima, T., Synthesis of high surface area hydroxyapatite nanoparticles by mixed surfactant-mediated approach (2005) Langmuir, 21, pp. 4724-4728; 
Balasundaram, G., Sato, M., Webster, T.J., Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD (2006) Biomaterials, 27, pp. 2798-2805; 
Wutticharoenmongkol, P., Sanchavanakit, N., Pavasant, P., Supaphol, P., Novel bone scaffolds or electrospun polycaprolactone fibers filled with nanoparticles (2006) J Nanosci Nanotechnol, 6, pp. 514-522; 
Gonzalez-McQuire, R., Green, D., Walsh, D., Hall, S., Chane-Ching, J., Oreffo, R.O.C., Mann, S., Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating (2005) Biomaterials, 26, pp. 6652-6656; 
Sun, W.B., Wu, Y.F., Ding, Y., Chu, C.L., Liu, T.J., The expression and activity of alkaline phosphatase in human periodontal ligament cells with nanometer hydroxyapatite (2006) Zhonghua Kou Qiang Yi Xue Za Zhi, 41, pp. 348-349; 
Bowers, K., Keller, J.C., Randolph, B., Wick, D., Michaels, C., Optimization of surface micromorphology for enhanced osteoblast responses in vitro (1992) Int J Oral Maxillofac Impl, 7, pp. 302-310; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford Jr, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401; 
Boyan, B.D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D.L., Szmuckler-Monclers, S., Dean, D.D., Schwartz, Z., Titanium surface roughness alters responsiveness of MG63 osteoblastic-like cells to 1α,25-(OH) 2D 3 (1998) J Biomed Mater Res, 39, pp. 77-85
},
	month = {2008///},
	pages = {483 - 493},
	abstract = {This study concerns the preparation, physical, and in vitro characterization of two different types of hydroxyapatite (HA) microspheres, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using the chemical precipitation synthesis with H                        3PO
                        4, Ca(OH) 
                        2, and a surfactant, SDS (sodium dodecyl sulfate), as starting reagents. The HA powders were dispersed in a sodium alginate solution, and spherical particles were obtained by droplet extrusion coupled with ionotropic gelation in the presence of Ca
                        2+. These were subsequently sintered to produce HA-1 and HA-2 microspheres with a uniform size and interconnected microporosity. Both powders and microspheres were characterized using FTIR and X-ray diffraction. Moreover, SEM and mercury intrusion porosimetry were used to analyze the microspheres, and TEM was used to analyze the powders. Results showed that pure HA and mixtures of HA/β-TCP in the nanometer range and needlelike shape were obtained for HA-1 and HA-2 powders, respectively. Neutral Red, scanning electron microscopy and confocal microscopy were used to evaluate the behavior of osteoblastic-like MG-63 cells cultured on HA microspheres surfaces for 7 days. Results showed that good adhesion and proliferation of osteoblasts on the HA microspheres surface. Cells built bridges between adjacent microspheres, forming microspheres-cells clusters in both types of materials. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {alginic acid, analytic method, Apatite, article, Biocompatible Materials, Biological materials, biomaterial, Bone Regeneration, Calcium, calcium hydroxide, calcium phosphate, cell adhesion, Cell Line, cell proliferation, Chemical precipitation, Chemotherapy, Comparative studies, comparative study, controlled study, dodecyl sulfate sodium, Drug delivery, Drug delivery system (DDS), Drug Delivery Systems, Drug dosage, Durapatite, Gelation, human, human cell, Humans, hydroxyapatite, Hydroxyapatite (HAP), Hydroxylation, In vitro characterization, in vitro study, infrared spectroscopy, materials testing, Medical applications, Mercury intrusion porosimetry, microsphere, Microspheres, Nano-hydroxyapatite (n-HA), Nanohydroxyapatite, nanoparticle, nanoparticles, neutral red, osteoblast, Osteoblasts, Particle Size, phosphoric acid, porosity, powder, Powders, precipitation, Precipitation (chemical), reagent, Regeneration (renovating), Scanning electron microscopy, surfactant, synthesis, Synthesis (chemical), X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47049103112\&partnerID=40\&md5=138b3e2f3a97d8b398546d3f85567a0f},
	author = {Mateus, A.Y.P. and Barrias, C.C. and Ribeiro, C. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2335,
	title = {Data classification with multilayer perceptrons using a generalized error function},
	journal = {Neural NetworksNeural Netw.},
	volume = {21},
	year = {2008},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: NNETEdoi: 10.1016/j.neunet.2008.04.004PubMed ID: 18572384Language of Original Document: EnglishCorrespondence Address: Silva, L.M.; INEB-Instituto de Engenharia Biom{\'e}dica, Porto, Portugal; email: lmsilva@fe.up.ptReferences: Baum, E.B., Wilczek, F., Supervised learning of probability distributions by neural networks (1987) NIPS, pp. 52-61. , Anderson D.Z. (Ed), American Institute of Physics;Bishop, C., (1995) Neural networks for pattern recognition, , Oxford University Press; 
Blake, C., \& Merz, C. (1998). UCI repository of machine learning databases. University of California, Irvine, Dept. of Information and Computer SciencesForina, M., Armanino, C., Eigenvector projection and simplified non-linear mapping of fatty acid content of italian olive oils (1981) Annali di Chimica (Rome), 50, pp. 127-155; 
Gish, H. (1990). A probabilistic approach to the understanding and training of neural network classifiers. In Proceedings of the 1990 IEEE international conference on acoustics, speech, and signal processingHampshire, J., Waibel, A., A novel objective function for improved phoneme recognition using time-delay neural networks (1990) IEEE Transactions on Neural Networks, 1 (2), pp. 216-228; 
Hinton, G.E., Connectionist learning procedures (1989) Artificial Intelligence, 40 (1-3), pp. 185-234; 
Jacobs, R., Jordan, M., Nowlan, S., Hinton, G., Adaptive mixtures of local experts (1991) Neural Computation, 3, pp. 79-87; 
M{\o}ller, M. (1993). Efficient training of feed-forward neural networks. Ph.D. thesis. Computer Science Department - Aarhus UniversityMarques de S{\'a}, J., (2001) Pattern recognition: Concepts, methods and applications, , Springer Verlag; 
Matsuoka, K., \& Yi, J. (1991). Backpropagation based on the logarithmic error function and elimination of local minima. In Proceedings of the 1990 IEEE international joint conference on neural networksRichard, M., Lippmann, R., Neural network classifiers estimate bayesian a posteriori probabilities (1991) Neural Computation, 3, pp. 461-483; 
Santos, J., Alexandre, L., \& Marques~de S{\'a}, J. (2004). The error entropy minimization algorithm for neural network classification. In int. conf. on recent advances in soft computingSilva, L., Alexandre, L., Marques de S{\'a}, J., Neural network classification: Maximizing zero-error density (2005) LNCS, 3686, pp. 127-135. , ICAPR 2005; 
Silva, L., Alexandre, L.A., \& Marques~de S{\'a}, J. (2006). New developments of the Z-EDM algorithm. In Proceedings of the sixth international conference intelligent systems design and applications: Vol.~1 (pp. 1067-1072)Silva, L., Marques~de S{\'a}, J., \& Alexandre, L. (2005). Neural network classification using Shannon{\textquoteright}s entropy. In European symposium on artificial neural networksSilverman, B., (1986) Density estimation for statistics and data analysis, , Chapman \& Hall; 
Solla, S.A., Levin, E., Fleisher, M., Accelerated learning in layered neural networks (1988) Complex Systems, 2 (6), pp. 625-639
},
	month = {2008///},
	pages = {1302 - 1310},
	abstract = {The learning process of a multilayer perceptron requires the optimization of an error function E (y, t) comparing the predicted output, y, and the observed target, t. We review some usual error functions, analyze their mathematical properties for data classification purposes, and introduce a new one, E                        Exp, inspired by the Z-EDM algorithm that we have recently proposed. An important property of E
                        Exp is its ability to emulate the behavior of other error functions by the sole adjustment of a real-valued parameter. In other words, E
                        Exp is a sort of generalized error function embodying complementary features of other functions. The experimental results show that the flexibility of the new, generalized, error function allows one to obtain the best results achievable with the other functions with a performance improvement in some cases. {\textcopyright} 2008 Elsevier Ltd. All rights reserved.
},
	keywords = {algorithm, Algorithms, analytical error, article, calculation, Classification, Complementary features, Cybernetics, data analysis, Data classification, Data classifications, Data Interpretation, Statistical, Electric loads, entropy, Error functions, learning, Learning processes, mathematical analysis, Mathematical properties, Models, Statistical, Multilayer neural networks, Multilayer perceptron, Multilayer perceptrons, Multilayers, Neural networks, Neural Networks (Computer), Pattern recognition systems, perceptron, Performance improvements, priority journal, Probability density function},
	isbn = {08936080 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-54449100428\&partnerID=40\&md5=30f8de18c0272f4a440395045bd3c164},
	author = {Silva, L.M. and Marques de S{\'a}, J. and Alexandre, L.A.}
}
@article {2340,
	title = {Dissimilarity-based classification of chromatographic profiles},
	journal = {Pattern Analysis and ApplicationsPattern Anal. Appl.},
	volume = {11},
	year = {2008},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1007/s10044-008-0113-2Language of Original Document: EnglishCorrespondence Address: Sousa, A. V.; Instituto Superior de Engenharia do Porto, Rua Dr. Ant{\'o}nio Bernardino Almeida, 431, 4200-072 Porto, Portugal; email: ats@isep.ipp.ptReferences: Reich, E., Blatter, A., Modern TLC: A key technique for identification and quality control of botanicals and dietary supplements (2004) Inside Laboratory Management-AOC International;Sousa, A.V., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic lane and band detection in images of thin layer chromatography (2004) LNCS, 3212, pp. 158-165. , Proceedings of image analysis and recognition: international conference, ICIAR 2004, Porto Springer, Heidelberg; 
Keir, G., Winchester, B.G., Clayton, P., Carbohydrate-deficient glycoprotein syndromes: Inborn errors of protein glycosylation (1999) Ann Clin Biochem, 36, pp. 20-36. , Pt 1; 
Durand, G., Seta, N., Protein glycosylation and diseases: Blood and urinary oligosaccharides as markers for diagnosis and therapeutic monitoring (2000) Clin Chem, 46, pp. 795-805; 
Schwedt, G., (1997) The Essential Guide to Analytical Chemistry, , Wiley New York; 
Bajla, I., Hollander, I., Fluch, S., Burg, K., Kollar, M., An alternative method for electrophoretic gel image analysis in the GelMaster software (2005) Comput Methods Programs Biomed, 77, pp. 209-231; 
Eibrand, R., Kennedy, P., Cotter, D., MacEvilly, U., Wu, B., Analysis of atlantic salmon skin mucus: COPS-a computer-based system for protein pattern analysis of 1D SDS-PAGE gels (2003) Proceedings of the Third IEEE Symposium on Bioinformatics and Bioengeneering; 
Gerasimov, A.V., Use of the software processing of scanned chromatogram images in quantitative planar chromatography (2004) J Anal Chem, 59, pp. 348-353; 
(2000) Gel Analysis Software: Important Aspects, , Goulding electrophoresis PN UVItec Ltd; 
Jedra, M., El Khattabi, N., Limouri, M., Essaid, A., Recognition of seed varieties using a time-delay neural network: Analysis of electrophoretic images (1999) Comput Electron Agric, 22, pp. 1-10; 
MacHado, A.M.C., Campos, M.F.M., Siqueira, A.M., De Carvalho, O.S.F., An iterative algorithm for segmenting lanes in gel electrophoresis images. Computer graphics and image processing (1997) Proceedings of X Brazilian Symposium, pp. 140-146; 
Ye, X., Suen, C.Y., Cheriet, M., Wang, E., A recent development in image analysis of electrophoresis gels (1999) Vision Iterface 99, , Trois-Rivieres, Canada; 
Wang, D., Keller, J.M., Carson, C.A., Pulsed-field gel electrophoresis pattern recognition of bacterial DNA: A systemic approach (2001) Pattern Anal Appl, 4, pp. 244-255; 
Shadle, S.E., Allen, D.F., Guo, H., Pogozelski, W.K., Bashkin, J.S., Tullius, T.D., Quantitative analysis of electrophoresis data: Novel curve fitting methodology and its application to the determination of a protein-NA binding constant (1997) Nucleic Acids Res, 25, pp. 850-860; 
Bajla, I., Hollander, I., Burg, K., Improvement of electrophoretic gel image analysis (2001) Measurement Science Review, 1; 
Bajla, I., Hollander, I., Burg, K., Fluch, S., (2002) A Novel Approach to Quantitative Analysis of Electrophoretic Gel Images of DNA Fragments, pp. 899-902. , IEEE international symposium on biomedical imaging Washington; 
Lavine, B.K., Meyers, R.A., Clustering and classification of analytical data (2000) Encyclopedia of Analytical Chemistry: Instrumentation and Applications, pp. 9689-9710. , Wiley Chichester; 
Lonni, A., Scarminio, I., Silva, L., Ferreira, D., Differentiation of species of bacharis genus by HPLC and chemometrics methods (2003) Analytical Sciences, 19; 
Beltr{\'a}n, N.H., Duarte-Mermoud, M.A., Salah, S.A., Bustos, M.A., Feature selection algorithms using Chilean wine chromatograms as examples (2005) J Food Eng, 67, pp. 483-490; 
Landgrebe, D., Information extraction principles and methods for multispectral and hyperspectral image data (1999) Proceedings of Information Processing for Remote Sensing, , World Scientific, Singapore; 
Landgrebe, D., Hyperspectral image data analysis as a high dimensional signal processing problem (2002) IEEE Signal Process Mag, 19, pp. 17-28; 
Jimenez, L., Landgrebe, D., Hyperspectral data analysis and feature reduction via projection pursuit (1999) IEEE Trans Geosci Remote Sens, 37, pp. 2653-2667; 
Jimenez, L.O., Landgrebe, D.A., Supervised classification in high-dimensional space: Geometrical, statistical, and asymptotical properties of multivariate data (1998) IEEE Transaction on Systems Man, and Cybernetics-Part C: Applications and Reviews, 28; 
Paclik, P., Duin, R.P.W., Dissimilarity-based classification of spectra: Computational issues (2003) Real-Time Imaging, 9, pp. 237-244; 
Paclik, P., Duin, R.P.W., Classifying spectral data using relational representation (2003) Proceedings of Spectral Imaging Workshop, , Graz; 
Pekalska, E., Paclik, P., Duin, R.P.W., A generalized kernel approach to dissimilarity-based classification (2001) J Mach Learn Res, 2, pp. 175-211; 
Pekalska, E., Duin, R.P.W., Dissimilarity representations allow for building good classifiers (2002) Pattern Recogn Lett, 23, pp. 943-956; 
Pekalska, E., Duin, R.P.W., (2005) The Dissimilarity Representation for Pattern Recognition-foundations and Applications, 64. , World Scientific, Singapore; 
Pekalska, E., Duin, R.P.W., Paclik, P., Prototype selection for dissimilarity-based classifiers (2006) Pattern Recogn, 39, pp. 189-208; 
Harol, A., Lai, C., Pekalska, E., Duin, R.P.W., Pairwise feature evaluation for constructing reduced representations (2007) Pattern Anal Appl, 10, pp. 55-68; 
Dubuisson, M.-P., Jain, A.K., A modified Hausdorff distance for object matching (1994) Proceedings of International Conference on Pattern Recognition, pp. 566-568. , Jerusalem; 
Heijden, F., Robert, P.W.D., Ridder, D., Tax, D.M.J., (2004) Classification, Parameter Estimation and State Estimation, , Wiley, New York; 
Dasarathy, B.V., S{\'a}nchez, J.S., Townsend, S., Nearest neighbour editing and condensing tools-synergy exploitation (2000) Pattern Anal Appl, 3, pp. 19-30; 
Devijver, P., Kittler, J., (1982) Pattern Recognition: A Statistical Approach, , Prentice-Hall, New Jersey; 
Cheng, Y., Mean shift, mode seeking, and clustering (1995) IEEE Trans Pattern Anal Mach Intell, 17, pp. 790-799; 
Vijaya, P.A., Murty, M.N., Subramanian, D.K., Efficient median based clustering and classification techniques for protein sequences (2006) Pattern Anal Appl, 9, pp. 243-255; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Aguiar, R., Miranda, C.S., Feature extraction for classification of thin-layer chromatography images (2005) Proceedings of Image Analysis and Recognition, Second International Conference, ICIAR 2005, 3656, pp. 974-981. , LNCS Springer, Toronto; 
Dempster, A., Laird, N., Rubin, D., Maximum likelihood from incomplete data via the em algorithm (1977) J R Stat Soc, B-39, pp. 1-38. , 1; 
Mor{\'e}, J.J., Sorensen, D.C., Computing a trust region step (1983) SIAM J Sci Stat Comput, 4, pp. 553-572; 
Byrd, R.H., Schnabel, R.B., Shultz, G.A., Approximate solution of the trust region problem by minimization over two-dimensional subspaces (1988) Math Program, 40, pp. 247-263; 
Visa, S., Ralescu, A., Learning imbalanced and overlapping classes using fuzzy sets (2003) Workshop on Learning from Imbalanced Datasets II, , ICML, Washington DC; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A.C., The class imbalance problem in TLC image classification (2006) Proceedings of Image Analysis and Recognition, Third International Conference, ICIAR 2006, 4142, pp. 513-523. , September 18-20, 2006, proceedings, Part II, P{\'o}voa de Varzim. LNCS Springer, Portugal
},
	month = {2008///},
	pages = {409 - 423},
	abstract = {This paper proposes a non-parametric method for the classification of thin-layer chromatographic (TLC) images from patterns represented in a dissimilarity space. Each pattern corresponds to a mixture of Gaussian approximation of the intensity profile. The methodology comprises various phases, including image processing and analysis steps to extract the chromatographic profiles and a classification phase to discriminate among two groups, one corresponding to normal cases and the other to three pathological classes. We present an extensive study of several dissimilarity-based approaches analysing the influence of the dissimilarity measure and the prototype selection method on the classification performance. The main conclusions of this paper are that, Match and Profile-difference dissimilarity measures present better results, and a new prototype selection methodology achieves a performance similar or even better than conventional methods. Furthermore, we also concluded that simplest classifiers, such as k-NN and linear discriminant classifiers (LDCs), present good performance being the overall classification error less than 10\% for the four-class problem. {\textcopyright} 2008 Springer-Verlag London Limited.},
	keywords = {Chromatographic images, Dissimilarity representation, Nearest neighbour classifiers, Prototype selection},
	isbn = {14337541 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-50549105086\&partnerID=40\&md5=2a5bd02f0caacb5e7ccb1267a861db75},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2346,
	title = {A hybrid approach for arabidopsis root cell image segmentation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5112 LNCS},
	year = {2008},
	note = {Conference code: 72695Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-540-69812-8_73Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, pp. 13-13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic Tracking of Arabidopsis thaliana Root Meristem in Confocal Microscopy (2004) LNCS, 3212, pp. 166-174. , Campilho, A.C, Kamel, M, eds, ICIAR 2004, Springer, Heidelberg; 
Perona, P., Malik, J., Scale-space and edge detection using anisotropic diffusion (1990) IEEE Trans. on PAMI, 7 (12), pp. 629-639; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. on PAMI, 13 (6), pp. 583-598; 
Meyer, F., Beucher, S., Morphological segmentation (1990) JVCIR, 1 (1), pp. 21-46; 
Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Underst, 77 (3), pp. 317-370; 
Kovesi, P.: Symmetry and asymmetry from local phase. In: Proc. of the Tenth Australian Joint Conf. on Artificial Intelligence (December 1997)Burges, C., A tutorial on support vector machines for pattern recognition (1998) Data Mining and Knowledge Discovery, 2 (2), pp. 121-167
},
	month = {2008///},
	pages = {739 - 749},
	address = {Povoa de Varzim},
	abstract = {In vivo observation and tracking of the Arabidopsis thaliana root meristem, by time-lapse confocal microscopy, is important to understand mechanisms like cell division and elongation. The research herein described is based on a large amount of image data, which must be analyzed to determine the location and state of cells. The automation of the process of cell detection/marking is an important step to provide research tools for the biologists in order to ease the search for special events, such as cell division. This paper discusses a hybrid approach for automatic cell segmentation, which selects the best cell candidates from a starting watershed-based image segmentation and improves the result by merging adjacent regions. The selection of individual cells is obtained using a Support Vector Machine (SVM) classifier, based on the shape and edge strength of the cells{\textquoteright} contour. The merging criterion is based on edge strength along the line that connects adjacent cells{\textquoteright} centroids. The resulting segmentation is largely pruned of badly segmented and over-segmented cells, thus facilitating the study of cell division. {\textcopyright} 2008 Springer-Verlag Berlin Heidelberg.},
	keywords = {Arabidopsis (Arabidopsis thaliana), Arabidopsis thaliana (ELIP), Cell divisions, Cell image segmentation, cell proliferation, Cell segmentation, Cells, confocal microscopy, Digital image storage, Heidelberg (CO), Hybrid approaches, Image analysis, Image data, image processing, Image segmentation, Imaging techniques, In vivo observation, Individual (PSS 544-7), International conferences, Merging, Microscopic examination, Research tools, Root meristem, Strength (IGC: D5/D6), Support Vector Machine (SVM), Support vector machines, Time lapse confocal microscopy},
	isbn = {03029743 (ISSN); 3540698116 (ISBN); 9783540698111 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47749134578\&partnerID=40\&md5=3b05fc0d5d004216e681d9c3e2199aac},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2362,
	title = {Hybrid chitosan membranes tested in sheep for guided tissue regeneration},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {361-363 II},
	year = {2008},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Cortez, P.P.; Centro de Estudos de Ci{\^e}ncia Animal (CECA), Instituto de Ci{\^e}ncias e Tecnologias Agr{\'a}rias e Agro-Alimentares (ICETA), Campus Agr{\'a}rio de Vair{\~a}o, Rua P. Armando Quintas, 4485-661, Vair{\~a}o, Portugal; email: pcortez@mail.icav.up.ptReferences: Nasser, N.J., Friedman, A., Friedman, M., Moor, E., Mosheiff, R., (2005) Injury, 36, p. 1460;Chandy, T., Sharma, C.P., (1990) Biomater. Artif. Cells Artif. Organs, 18, p. 1; 
Domard, A., Rinaudo, M., (1983) Int J Biol Macromol, 5, p. 49; 
Shirosaki, Y., Tsuru, K., Hayakawa, S., Osaka, A., Lopes, M.A., Santos, J.D., Fernandes, M.H., (2004) Key Eng. Mat, 254-256, p. 857; 
Shirosaki, Y., Tsuru, K., Hayakawa, S., Osaka, A., Lopes, M.A., Santos, J.D., Fernandes, M.H., (2005) Biomaterials, 26, p. 485; 
Ueno, H., Yamada, H., Tanaka, I., Kaba, N., Matsuura, M., Okumura, M., Kadosawa, T., Fujinaga, T., (1999) Biomaterials, 20, p. 1407
},
	month = {2008///},
	pages = {1265 - 1268},
	abstract = {Previous in vitro studies confirmed an improved cytocompatibility of chitosan-silicate hybrid membranes over chitosan membranes. The main goal of this study was to assess the in vivo histocompatibility of both membranes through subcutaneous implantations at different time periods, 1 week, 1, 2 and 3 months, using a sheep model. Chitosan membranes elicited an exuberant inflammatory response and were consequently rejected. The hybrid chitosan membranes were not rejected and the degree of inflammatory response decreased gradually until the third month of implantation. Histological evaluation also showed that these membranes can be resorbed in vivo. This study demonstrates that the incorporation of silicate into the chitosan solution improves its histocompatibility, indicating that the hybrid chitosan-silicate membranes are suitable candidates to be used in clinical applications.},
	keywords = {biodegradability, Biodegradable membrane, Biodegradable membranes, chitosan, Chitosan-silicate hybrid, Guided tissue regeneration (GTR), histology, in vivo study, Membranes, Silicates, Tissue culture},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38149018476\&partnerID=40\&md5=9a6734a40aa84667fe014796596e1ef3},
	author = {Cortez, P.P. and Shirosaki, Y. and Botelho, C.M. and Sim{\~o}es, M.J. and Gartner, F. and Da Costa, R.M.G. and Tsuru, K. and Hayakawa, S. and Osaka, A. and Lopes, M.A. and Santos, J.D. and Maur{\'\i}cio, A.C.}
}
@article {2366,
	title = {Jaw avascular osteonecrosis after treatment of multiple myeloma with zoledronate},
	journal = {Journal of Plastic, Reconstructive and Aesthetic SurgeryJ. Plast. Reconstr. Aesthetic Surg.},
	volume = {61},
	year = {2008},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.bjps.2006.06.016PubMed ID: 18068658Language of Original Document: EnglishCorrespondence Address: Maur{\'\i}cio, A.C.; Centro de Estudos de Ci{\^e}ncia Animal (CECA), Instituto de Ci{\^e}ncias, Tecnologias Agr{\'a}rias e Agro-Alimentares (ICETA), Campus Agrario Vairao, Rua Padre Armando Quintas, 4485-661 Vair{\~a}o, Portugal; email: ana.colette@mail.icav.up.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; cyclophosphamide, 50-18-0; cyclosporin, 79217-60-0; dexamethasone, 50-02-2; erythropoietin, 11096-26-7; thalidomide, 50-35-1; zoledronic acid, 118072-93-8, 131654-46-1, 165800-06-6, 165800-07-7; Bone Density Conservation Agents; Diphosphonates; Imidazoles; zoledronic acid, 118072-93-8References: Mundy, G.R., Bertoline, D.R., Bone destruction and hypercalcemia in plasma cell myeloma (1986) Semin Oncol, 13 (3), pp. 291-299;Belch, A.R., Bergsagel, D.E., Wilson, K., Effect of daily etidronate on the osteolysis of multiple myeloma (1991) J Clin Oncol, 9 (8), pp. 1397-1402; 
Kyle, R.A., Jowsey, J., Kelly, P.J., Multiple myeloma bone disease. The comparative effect of sodium fluoride and calcium carbonate or placebo (1975) N Engl J Med, 293 (26), pp. 1334-1338; 
Kyle, R.A., Multiple myeloma: review of 869 cases (1975) Mayo Clin Proc, 50 (1), pp. 29-40; 
Roodman, G.D., Pathogenesis of myeloma bone disease (2004) Blood Cells Mol Dis, 32 (2), pp. 290-292; 
Berenson, J.R., Myeloma bone disease (2005) Best Pract Res Clin Haematol, 18 (4), pp. 653-672; 
Ruggerio, S.L., Mekrotra, B., Rosenberg, T.J., Osteonecrosis of the jaws associated with the use of bisphosphonates: a review of 63 cases (2004) J Oral Maxillofac Surg, 62, pp. 527-534; 
Rodan, G.A., Fleisch, H.A., Bisphosphonates: mechanism of action (1996) J Clin Invest, 97 (12), pp. 2692-2696; 
Coleman, R.E., Metastatic bone disease: clinical features, pathophysiology and treatment strategies (2001) Cancer Treat Rev, 27 (3), pp. 165-176; 
Sietsema, W.K., Ebetino, F.H., Salvagno, A.M., Antiresorptive dose-response relationship across three generations of bisphosphonates (1989) Drugs Exp Clin Res, 15 (9), pp. 389-396; 
Schenk, R., Eggli, P., Fleisch, H., Quantitative morphometric evaluation of the inhibitory activity of new amino-bisphosphonates on bone resorption in the rat (1986) Calcif Tissue Int, 38, pp. 342-349; 
Jung, A., Bisaz, S., Fleisch, H., The binding of pyrophosphate and two diphosphonates by hydroxyapatite crystals (1973) Calcif Tissue Res, 11 (4), pp. 269-280; 
Coukell, A.J., Markham, A., Pamidronate. A review of its use in the management of osteolytic bone metastases, tumor-induced~hypercalcaemia and Paget{\textquoteright}s disease of bone (1998) Drugs Aging, 12 (2), pp. 149-168; 
Hughes, D.E., MacDonald, B.R., Russel, R.G.G., Inhibition of osteoclast-like cell formation by bisphosphonates in long-term cultures of human bone marrow (1989) J Clin Invest, 83 (6), pp. 1930-1935; 
Hughes, D.E., Wright, K.R., Uy, H.L., Bisphosphonates promote apoptosis in murine osteoclasts in vitro and in vivo (1995) J Bone Miner Res, 10 (10), pp. 1478-1487; 
Murakami, H., Takahashi, N., Sasaki, T., A possible mechanism of the specific action of bisphosphonates on osteoclasts: tiludronate preferentially affects polarized osteoclasts having ruffled borders (1995) Bone, 17 (2), pp. 137-144; 
Sahni, M., Guenther, H.L., Fleisch, H., Bisphosphonates act on rat bone resorption through the mediation of osteoblasts (1993) J Clin Invest, 91 (5), pp. 2004-2011; 
Fleisch, H., Bisphosphonates: mechanisms of action (1998) Endocr Rev, 19 (1), pp. 80-100; 
Shinoda, H., Adamek, G., F{\'e}lix, R., Structure-activity relationships of various bisphosphonates (1983) Calcif Tissue Int, 35 (1), pp. 87-99; 
Pereira, J., Mancini, I., Walker, P., The role of bisphosphonates in malignant bone pain: a review (1998) J Palliat Care, 14 (2), pp. 25-36; 
Purohit, O.P., Radstone, C.R., Anthony, C., A randomized double-blind comparison of intravenous pamidronate and clodronate in the hypercalcemia of malignancy (1995) Br J Cancer, 72 (5), pp. 1289-1293; 
Hillner, B.E., Ingle, J.N., Chlebowski, R.T., American Society of Clinical Oncology 2003 update on the role of bisphosphonates and bone health issues in women with breast cancer (2003) J Clin Oncol, 21 (21), pp. 4042-4057. , [Erratum in J Clin Oncol 2004;22(7):1351]; 
Hillner, B.E., Ingle, J.N., Berenson, J.R., American Society of Clinical Oncology guideline on the role of bisphosphonates in breast cancer. American Society of Clinical Oncology Bisphosphonates Expert Panel (2000) J Clin Oncol, 18 (6), pp. 1378-1391; 
Lacerna, L., Hohneker, J., Zoledronic acid for the treatment of bone metastases in patients with breast cancer and other solid tumours (2003) Semin Oncol, 30 (5 SUPPL. 16), pp. 150-160; 
Lipton, A., Costa, L., Ali, S., Use of markers of bone turnover for monitoring bone metastases and the response to therapy (2001) Semin Oncol, 28 (4 SUPPL. 11), pp. 54-59; 
Brincker, H., Westin, J., Abildgaard, N., Failure of oral pamidronate to reduce skeletal morbidity in multiple myeloma: a double-blind placebo-controlled trial. Danish-Swedish Co-operative Study Group (1998) Br J Haematol, 101 (2), pp. 3280-3286; 
Berenson, J.R., Lichtenstein, A., Porter, L., Long-term pamidronate treatment of advanced multiple myeloma patients reduces skeletal events. Myeloma Aredia Study Group (1998) J Clin Oncol, 16 (7), pp. 593-602; 
Berenson, J.R., Vescio, R.A., Lee, S.R., Phase I dose-ranging trial of monthly infusions of zoledronic acid for the treatment of osteolytic bone metastases (2001) Clin Cancer Res, 7 (3), pp. 478-485; 
Van Poznak, C.H., The use of bisphosphonates in patients with breast cancer (2002) Cancer Control, 9 (6), pp. 480-489; 
Marx, R.E., Pamidronate (Aredia) and zoledronate (Zometa) induced avascular necrosis of the jaws: a growing epidemic (2003) J Oral Maxillofac Surg, 61 (9), pp. 1115-1117; 
Vannucchi, A.M., Ficarra, G., Antonioli, E., Osteonecrosis of the jaw associated with zoledronate therapy in patient with multiple myeloma (2005) Br J Haematol, 128 (6), pp. 738-739; 
Wang, J., Goodger, N.M., Pogrel, M.A., Osteonecrosis of the jaws associated with cancer chemotherapy (2003) J Oral Maxillofac Surg, 61 (9), pp. 1104-1107; 
Purcell, P.M., Boyd, I.W., Bisphosphonates and osteonecrosis of the jaw (2005) Med J Aust, 182 (8), pp. 417-418; 
Carter, G., Goss, A.N., Doecke, C., Bisphosphonates and avascular necrosis of the jaw: a possible association (2005) Med J Aust, 182 (8), pp. 413-415; 
Durie, B., Katz, M., Crowley, J., Osteonecrosis of the jaw and bisphosphonates (2005) N Engl J Med, 353, pp. 99-102; 
Migliorati, C.A., Bisphosphonates and oral cavity avascular bone necrosis (2003) J Clin Oncol, 21 (22), pp. 4253-4254; 
Robinson, N.A., Yeo, J.F., Bisphosphonates - a word of caution (2004) Ann Acad Med Singapore, 33 (4), pp. 48-49; 
Starck, W.J., Epker, B.N., Failure of osseointegrated dental implants after diphosphonate therapy for osteoporosis: a case report (1995) Int J Oral Maxillofac Implants, 10 (1), pp. 74-78; 
Melo, M., Obeid, G., Osteonecrosis of the maxilla in a patient with a history of bisphosphonate therapy (2005) J Can Dent Assoc, 71 (2), pp. 111-113
},
	month = {2008///},
	pages = {99 - 106},
	abstract = {Purpose: Multiple myeloma, the second most common haematopoietic cancer, which represents the collection of plasma-cell neoplasms that invariably becomes fatal when self-renewing myeloma cells begin unrestrained proliferation. The major clinical manifestation of multiple myeloma is related to the loss of bone through osteolysis. This can lead to pathologic fractures, spinal cord compression, hypercalcaemia, and pain. It is also a major cause of morbidity and mortality in these patients, who frequently require radiation therapy, surgery and analgesic medications. Bisphosphonates are specific inhibitors of osteoclastic activity, and are currently used to prevent bone complications and to treat malignant hypercalcaemia in patients with multiple myeloma, or bone metastases from breast and prostate cancers. Hence, osteonecrosis of the mandible has been reported in three patients from Centro Hospitalar de Vila Nova de Gaia (CHVNG) with multiple myeloma treated for over 18-48 months with intravenous zoledronate, commonly prescribed for multiple myeloma therapy. Although, this report alerts clinicians about the potential complication of bone necrosis in patients receiving bisphosphonate therapy, many questions remain concerning the underlying pathogenesis of this process. Patients and methods: The medical and dental records of three patients with multiple myeloma, who were treated in CHVNG in the past 4 years, were reviewed. These three patients presented exposed bone and osteonecrosis of the mandible, and shared one common clinical feature: all of them were treated with bisphosphonate zoledronate, administered intravenously for long periods. Sequential orthopantomograms (OPGs) and histological evaluation have been analysed from the biopsies of the non healing dental extraction sites of these patients. Results: After a routine dental extraction, these patients developed avascular osteonecrosis of the mandible and secondary bone infection with Actinomyces israelii (actinomycotic osteomyelitis), with no evidence of metastatic disease evaluated by biopsy. In these three described clinical cases, surgical debridment without flap elevation, intensive antibiotherapy and the suspension of the zoledronate treatment allowed a partial recovery of the patients. The purpose of this clinical report is to point out that patients suffering from multiple myeloma can develop bone osteonecrosis induced by treatment with bisphosphonates. Research to determine the mechanism of this dental phenomenon is needed to fully validate and substantiate the possible link between bisphosphonate treatment of multiple myeloma or other cancer diseases and avascular osteonecrosis of the jaws. Until then, clinicians involved in the care of patients at risk should consider this possible complication. {\textcopyright} 2006 British Association of Plastic, Reconstructive and Aesthetic Surgeons.},
	keywords = {Actinomyces israelii, adult, aged, amoxicillin, antibiotic therapy, article, avascular necrosis, Avascular osteonecrosis, biopsy, Bisphosphonates, Bone Density Conservation Agents, case report, clinical feature, cyclophosphamide, cyclosporin, dexamethasone, Diphosphonates, drug induced disease, drug inhibition, drug withdrawal, erythropoietin, Female, histology, human, Humans, hypercalcemia, Imidazoles, Jaw, jaw avascular osteonecrosis, male, Mandibular Diseases, morbidity, mortality, Multiple myeloma, osteolysis, Osteonecrosis, pain, panoramic radiography, pathologic fracture, priority journal, Radiography, Panoramic, risk assessment, spinal cord compression, thalidomide, tooth extraction, zoledronic acid},
	isbn = {17486815 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-36649035515\&partnerID=40\&md5=3cc23473fdb164be7f8e067bea9e436e},
	author = {Lobato, J.V. and Maur{\'\i}cio, A.C. and Rodrigues, J.M. and Cavaleiro, M.V. and Cortez, P.P. and Xavier, L. and Botelho, C. and Hussain, N.S. and Santos, J.D.}
}
@book {2329,
	title = {A list scheduling algorithm for scheduling multi-user jobs on clusters},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5336 LNCS},
	year = {2008},
	note = {Conference code: 75089Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-540-92859-1_13Language of Original Document: EnglishCorrespondence Address: Barbosa, J.; Faculdade de Engenharia, Departamento de Engenharia Inform{\'a}tica, Universidade Do Porto, Rua Dr. Roberto Frias, Porto 4200-465, Portugal; email: jbarbosa@fe.up.ptReferences: Amoura, A.K., Bampis, E., K{\"o}nig, J.-C., Scheduling algorithms for parallel gaussian elimination with communication costs (1998) IEEE Transactions on Parallel and Distributed Systems, 9 (7), pp. 679-686;Barbosa, J., Morais, C., Nobrega, R., Monteiro, A.P., Static scheduling of dependent parallel tasks on heterogeneous clusters. In: Heteropar 2005 (2005) IEEE Computer Society, pp. 1-8. , Los Alamitos; 
Barbosa, J., Padilha, A.J., Algorithm-dependent method to determine the optimal number of computers in parallel virtual machines (1999) LNCS, 1573, pp. 508-521. , Hern{\'a}ndez, V, Palma, J.M.L.M, Dongarra, J, eds, VECPAR 1998, Springer, Heidelberg; 
Barbosa, J., Tavares, J., Padilha, A.J., Linear algebra algorithms in a heterogeneous cluster of personal computers (2000) Proceedings of 9th Heterogeneous Computing Workshop, pp. 147-159. , IEEE CS Press, Los Alamitos; 
Berman, F., Wolski, R., Figueira, S., Schopf, J., Shao, G., Application-level scheduling on distributed heterogeneous networks (1996) Supercomputing 1996; 
Blazewicz, J., Dell{\textquoteright}Olmo, P., Drozdowski, M., Maczka, P., Scheduling multiprocessor tasks on parallel processors with limited availability (2003) European journal of Operational Research, 149, pp. 377-389; 
Blazewicz, J., Machowiak, M., Weglarz, J., Kovalyov, M., Trystram, D., Scheduling malleable tasks on parallel processors to minimize the makespan (2004) Annals of Operations Research, 129, pp. 65-80; 
Shivle, S., Mapping of subtasks with multiple versions in a heterogeneous ad hoc grid environment (2004) Heteropar 2004. IEEE Computer Society, , Los Alamitos; 
Kim, J.-K., Dynamically mapping tasks with priorities and multiple deadlines in a heterogeneous environment (2007) Journal of Parallel and Distributed Computing, 67, pp. 154-169; 
Geijn, R., Watts, J., Summa: Scalable universal matrix multiplication algorithm (1995), Technical Report CS-95-286, University of Tennessee, KnoxvilleGerasoulis, A., Yang, T., On the granularity and clustering of directed acyclic task graphs (1993) IEEE Transactions on Parallel and Distributed Systems, 686-701. , June; 
Jansen, K., Scheduling malleable parallel tasks: An asymptotic fully polynomial time approximation scheme (2004) Algorithmica, 39, pp. 59-81; 
Kwok, Y., Ahmad, I., Static scheduling algorithms for allocating directed task graphs to multiprocessors (1999) ACM Computing Surveys, 31 (4), pp. 406-471; 
Kwok, Y., Ahmad, I., On multiprocessor task scheduling using efficient state space search approaches (2005) Journal of Parallel and Distributed Computing, 65, pp. 1515-1532; 
Lep{\`e}re, R., Mouni{\'e}, G., Trystram, D., An approximation algorithm for scheduling trees of malleable tasks (2002) European journal of Operational Research, 142, pp. 242-249; 
Oh-Heum, C.K.-Y., Scheduling parallel tasks with individual deadlines (1999) Theoretical Computer Science, 215, pp. 209-223; 
Park, G.-L., Performance evaluation of a list scheduling algorithm in distributed memory multiprocessor systems (2004) Future Generation Computer Systems, 20, pp. 249-256; 
Shirazi, B., Wang, M., Pathak, G., Analysis and evaluation of heuristic methods for static task scheduling (1990) Journal of Parallel and Distributing Computing, 10, pp. 222-232; 
Sinnen, O., Sousa, L., List scheduling: Extension for contention awareness and evaluation of node priorities for heterogeneous cluster architectures (2004) Parallel Computing, 30, pp. 81-101; 
Topcuoglu, H., Hariri, S., Wu, M.-Y., Performance-effective and low-complexity task scheduling for heterogeneous computing (2002) IEEE Transactions on Parallel and Distributed Systems, 13 (3), pp. 260-274; 
Trystram, D., Scheduling parallel applications using malleable tasks on clusters (2001) 15th International Conference on Parallel and Distributed Processing Symposium
Sponsors: Pole de Competivite "Aerospace Valley"; European Centre for Research and Advanced Training in ENSEEIHT; Institut National Polytechmique de Toulouse; Institut de Recherche en Informatique de Toulouse; Universidade do Porto; Universite Paul Sabatier},
	month = {2008///},
	pages = {123 - 136},
	address = {Toulouse},
	abstract = {This paper addresses the problem of scheduling multi-user jobs on clusters, both homogeneous and heterogeneous. A user job is composed by a set of dependent tasks and it is described by a direct acyclic graph (DAG). The aim is to maximize the resource usage by allowing a floating mapping of processors to a given job, instead of the common mapping approach that assigns a fixed set of processors to a user for a period of time. The simulation results show a better cluster usage. The scheduling algorithm minimizes the total length of the schedule (makespan) of a given set of parallel jobs, whose priorities are represented in a DAG. The algorithm is presented as producing static schedules although it can be adapted to a dynamic behavior as discussed in the paper. {\textcopyright} 2008 Springer Berlin Heidelberg.},
	keywords = {Cluster computing, Dynamic behaviors, Electric load shedding, High performance liquid chromatography, List scheduling, Paper addresses, Parallel algorithms, Parallel task, Resource usage, Scheduling, Scheduling algorithms, Simulation results, Static and dynamic scheduling, Total length},
	isbn = {03029743 (ISSN); 3540928588 (ISBN); 9783540928584 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58449125218\&partnerID=40\&md5=2287e7e01d8bab29c2c0bea44372233b},
	author = {Barbosa, J. and Monteiro, A.P.}
}
@book {2343,
	title = {Minimizing the imbalance problem in chromatographic profile classification with one-class classifiers},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5112 LNCS},
	year = {2008},
	note = {Conference code: 72695Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-540-69812-8_41Language of Original Document: EnglishCorrespondence Address: Sousa, A. V.; Instituto de Engenharia Biom{\'e}dica, Rua Roberto Frias, 4200-465 Porto, Portugal; email: ats@isep.ipp.ptReferences: Keir, G., Winchester, B.G., Clayton, P., Carbohydrate-deficient Glycoprotein Syndromes: Inborn Errors of Protein Glycosylate (1999) Ann. Clin. Biochem, 36 (PART 1), pp. 20-36;Durand, G., Seta, N., Protein Glycosylation and Diseases: Blood and Urinary Oligosaccharides as Markers for Diagnosis and Therapeutic Monitoring (2000) Clin. Chem, 46, pp. 795-805; 
Scriver, S.R., Beaudet, A.L., Sly, W.S., Valle, D., (2001) The Metabolic Bases of Inherited Disease, 3. , MCGraw-Hill, New-York; 
Friedman, R.B., Williams, M.A., Moser, H.W., Kolodny, E.H., Improved Thin-Layer Chromatographic Method in the Diagnosis of Mannosidosis (1978) Clinical Chemistry, 24, pp. 1576-1577; 
Kurczynski, T.W., Kendzierski, K.S., Sewell, A.C., Kuczynski, T.W., Urinary Oligosaccharides in Pregnant or Lactating Women: Pitfall in Screening (1993) Clin. Chem, 39, pp. 2346-2347; 
Sewell, A.C., Urinary Oligosaccharides. Techniques in Diagnostic Human (1991) Biochemical Genetics, 219-231; 
Duvdevani-Bar, S., Edelman, S., Visual Recognition and Categorization on the Basis of Similarities to Multiple Class Prototypes (1999) Int. J. Comput. Vision, 33, pp. 201-228; 
Pekalska, E., Duin, R.P.W., Dissimilarity Representations Allow for Building Good Classifiers (2002) Pattern Recogn. Lett, 23, pp. 943-956; 
Goldstone, R.L., Son, J.Y., Similarity (2005) Cambridge Handbook of Thinking and Reasoning, pp. 13-36. , Holyoak, K, Morrison, R, eds, Cambridge University Press, Cambridge; 
Pekalska, E., Duin, R.P.W., (2005) The Dissimilarity Representation for Pattern Recognition -Fundations and Applications, 64. , World Scientific Publishing, Singapore; 
Lavine, B.K., Clustering and Classification of Analytical Data (2000) Encyclopedia of Analytical Chemistry: Instrumentation and Applications, pp. 9689-9710. , Meyers, R.A, ed, John Wiley \& Sons, Chichester; 
Kubat, M., Holte, R., Matwin, S.: Learning when Negative Examples Abound. In: van Someren, M., Widmer, G. (eds.) ECML 1997. LNCS, 1224, pp. 146-153. Springer, Heidelberg (1997)Skurichina, M., Raudys, S., Duin, R.P.W., K-Nearest Neighbors Directed Noise Injection in Multilayer Perceptron Training (2000) IEEE Transactions on Neural Networks, 11; 
Chawla, N.V., Japkowicz, N., Kolcz, A., Editorial: Special Issue: Learning from Imbalanced Data Sets (2004) Sigkdd Explorations, 6, pp. 1-6; 
Tax, D.M.J., (2001) One-class Classification; Concept-learning in the Absence of Counter-examples, , Ph.D. thesis: Delft University of Technology; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Chromatographic Pattern Classification (2007) IEEE Trans. on Biomedical Engineering, , doi:10.1109/TBME.2008.919852; 
Paclik, P., Duin, R.P.W., Dissimilarity-based Classification of Spectra: Computational Issues (2003) Real-Time Imaging, 9, pp. 237-244; 
Paclik, P., Duin, R.P.W., Classifying Spectral Data using Relational Representation (2003) Proc. of Spectral Imaging Workshop, , Graz, Austria; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Dissimilarity-based Classification of Chromatographic Profiles (2007) Pattern Analysis and Applications, , doi:10.1007/sl0044-008-0113-2; 
Pekalska, E., Paclik, P., Duin, R.P.W., A Generalized Kernel Approach to Dissimilarity-based Classifcation (2001) Journal of Machine Learning Research, 2, pp. 175-211; 
Ripley, B.D., (2004) Pattern Recognition and Neural Networks, , Cambridge University Press, Cambridge; 
Visa, S., Ralescu, A., Learning Imbalanced and Overlapping Classes Using Fuzzy Sets (2003) Workshop on Learning from Imbalanced Datasets II, ICML, , Washington DC
},
	month = {2008///},
	pages = {413 - 422},
	address = {Povoa de Varzim},
	abstract = {This paper presents a new classification approach to deal with class imbalance in TLC patterns, which is due to the huge difference between the number of normal and pathological cases as a consequence of the rarity of LSD diseases. The proposed architecture is formed by two decision stages: the first is implemented by a one-class classifier aiming at recognizing most of the normal samples; the second stage is a hierarchical classifier which deals with the remaining outliers that are expected to contain the pathological cases and a small percentage of normal samples. We have also evaluated this architecture by a forest of classifiers, using the majority voting as a rule to generate the final classification. The results that were obtained proved that this approach is able to overcome some of the difficulties associated with class imbalance. {\textcopyright} 2008 Springer-Verlag Berlin Heidelberg.},
	keywords = {Class imbalances, Classification (of information), Classification approach, Classifiers, Heidelberg (CO), Hierarchical classifiers, Image analysis, Imaging techniques, Imbalance problems, International conferences, Learning systems, Majority voting, Pathological cases},
	isbn = {03029743 (ISSN); 3540698116 (ISBN); 9783540698111 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47849088119\&partnerID=40\&md5=df911723c51c6f540287fdd675267725},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2359,
	title = {Osteoblast adhesion and morphology on TiO                    2 depends on the competitive preadsorption of albumin and fibronectin
},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {84},
	year = {2008},
	note = {Cited By (since 1996): 34Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31201PubMed ID: 17607748Language of Original Document: EnglishCorrespondence Address: Sousa, S.R.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, R. do Campo Alegre, 823, 4150-180 Porto, Portugal; email: ssousa@ibmc.up.ptChemicals/CAS: fibronectin, 86088-83-7; human serum albumin, 9048-49-1; hydrogen peroxide, 7722-84-1; iodine 125, 14158-31-7, 22822-81-7; titanium dioxide, 1317-70-0, 1317-80-2, 13463-67-7, 51745-87-0; Albumins; Coloring Agents; Fibronectins; Iodine Radioisotopes; Neutral Red, 553-24-2; titanium dioxide, 13463-67-7; Titanium, 7440-32-6References: Anselme, K., Osteoblast adhesion on biomaterials (2000) Biomaterials, 21, pp. 667-681;Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkane-thiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Malmsten, M., Lassen, B., Competitive adsorption at hydrophobic surfaces from binary protein systems (1994) J Colloid Interface Sci, 166, pp. 490-498; 
Koenig, A.L., Gambillara, V., Grainger, D.W., Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates (2003) J Biomed Mater Res A, 64, pp. 20-37; 
Horbett, T.A., The role of adsorbed proteins in animal cell adhesion (1994) Colloids Surf B, 2, pp. 225-240; 
Huang, H.H., Ho, C.T., Lee, T.H., Lee, T.L., Liao, K.K., Chen, F.L., Effect of surface roughness of ground titanium on initial cell adhesion (2004) Biomol Eng, 21, pp. 93-97; 
Vitte, J., Benoliel, A.M., Pierres, A., Bongrand, P., Is there a predictable relationship between surface physical-chemical properties and cell behaviour at the interface? (2004) Eur Cell Mater, 7, pp. 52-63; 
Tsai, W.B., Grunkemeier, J.M., McFarland, C.D., Horbett, T.A., Platelet adhesion to polystyrene-based surfaces preadsorbed with plasmas selectively depleted in fibrinogen, fibronectin, vitronectin, or von Willebrand{\textquoteright}s factor (2002) J Biomed Mater Res, 60, pp. 348-359; 
Grainger, D.W., Pavon-Djavid, G., Migonney, V., Josefowicz, M., Assessment of fibronectin conformation adsorbed to polytetrafluoroethylene surfaces from serum protein mixtures and correlation to support of cell attachment in culture (2003) J Biomater Sci Polym Ed, 14, pp. 973-988; 
Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-terminated self-assembled monolayers (2005) Biomaterials, 26, pp. 3891-3899; 
Culp, L.A., Sukenik, C.N., Cell type-specific modulation of fibronectin adhesion functions on chemically-derivatized self-assembled monolayers (1998) J Biomater Sci Polym Ed, 9, pp. 1161-1176; 
Baugh, L., Vogel, V., Structural changes of fibronectin adsorbed to model surfaces probed by fluorescence resonance energy transfer (2004) J Biomed Mater Res A, 69, pp. 525-534; 
McClary, K.B., Ugarova, T., Grainger, D.W., Modulating fibroblast adhesion, spreading, and proliferation using self-assembled monolayer films of alkylthiolates on gold (2000) J Biomed Mater Res, 50, pp. 428-439; 
Sousa, S.R., Moradas-Ferreira, P., Melo, L.V., Saramago, B., Barbosa, M.A., Human serum albumin adsorption on TiO 2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Gehanno, V., Freitas, P.P., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Soares, J.C., Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Iodine-125. A Guide to Radioiodination Techniques. Little Chalfont, UK: Amersham Life Science; 1993. p 64Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
Sousa, S.R., Moradas-Ferreira, P., Barbosa, M., TiO 2 type influences fibronectin adsorption (2005) J Mater Sci Mater Med, 16, pp. 1173-1178; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., Attachment of cultured human bone cells to novel polymers (1999) J Biomed Mater Res, 44, pp. 1-11; 
McFarland, C.D., Thomas, C.H., DeFilippis, C., Steele, J.G., Healy, K.E., Protein adsorption and cell attachment to patterned surfaces (2000) J Biomed Mater Res, 49, pp. 200-210; 
Yang, Y.Z., Cavin, R., Ong, J.L., Protein adsorption on titanium surfaces and their effect on osteoblast attachment (2003) J Biomed Mater Res A, 67, pp. 344-349; 
MacDonald, D.E., Rapuano, B.E., Deo, N., Stranick, M., Somasundaran, P., Boskey, A.L., Thermal and chemical modification of titanium-aluminum-vanadium implant materials: Effects on surface properties, glycoprotein adsorption, and MG63 cell attachment (2004) Biomaterials, 25, pp. 3135-3146; 
Steele, J.G., Johnson, G., McFarland, C., Dalton, B.A., Gengenbach, T.R., Chatelier, R.C., Underwood, P.A., Griesser, H.J., Roles of serum vitronectin and fibronectin in initial attachment of human vein endothelial-cells and dermal fibroblasts on oxygen-containing and nitrogen-containing surfaces made by radiofrequency plasmas (1994) J Biomater Sci Polym Ed, 6, pp. 511-532; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res A, 66, pp. 247-259; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (1999) J Biomed Mater Res, 49, pp. 435-447; 
Webb, K., Hlady, V., Tresco, P.A., Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces (2000) J Biomed Mater Res, 49, pp. 362-368; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cell osteogenic differentiation and fibronectin adsorption on chitosan membranes: The effect of the degree of acetylation (2005) J Biomed Mater Res A, 75, pp. 387-397; 
Scotchford, C.A., Gilmore, C.P., Cooper, E., Leggett, G.J., Downes, S., Protein adsorption and human osteoblast-like cell attachment and growth on alkylthiol on gold self-assembled monolayers (2002) J Biomed Mater Res, 59, pp. 84-99; 
Pettit, D.K., Horbett, T.A., Hoffman, A.S., Influence of the substrate binding characteristics of fibronectin on corneal epithelial cell outgrowth (1992) J Biomed Mater Res, 26, pp. 1259-1275; 
Steele, J.G., Johnson, G., Underwood, P.A., Role of serum vitronectin and fibronectin in adhesion of fibroblasts following seeding onto tissue-culture polystyrene (1992) J Biomed Mater Res, 26, pp. 861-884
},
	month = {2008///},
	pages = {281 - 290},
	abstract = {This study aims at assessing the influence of the competitive preadsorption of human serum albumin (HSA) and human plasma fibronectin (FN) from binary solutions and 10\% plasma on MC3T3-E1 osteoblast adhesion and morphology on two types of TiO                        2 substrates. One was commercially pure titanium with a titanium oxide layer formed in an H
                        2O
                        2 solution and the other TiO
                        2 sputtered on Si (Sousa et al., Langmuir 2004; 20:9745-9754.). The strategy applied in the present investigation was to compare osteoblast adhesion to surfaces preadsorbed with HSA, FN, HSA/FN = 1, HSA/FN = 200, and 10\% plasma. The adsorption of proteins was evaluated measuring the amount and the effectiveness of binding with radiolabeled proteins, 
                        125I-FN and 
                        125I-HSA. Our results indicated that MC3T3-E1 osteoblast adhesion correlates well with the amounts of FN and HSA adsorbed on TiO
                        2 surfaces. Also, we found that fewer osteoblasts adhered to both substrates preadsorbed with HSA, HSA/FN = 200, and 10\% plasma, after 4 and 24 h, than to the surfaces preadsorbed with FN and HSA/FN = 1. For the latter, FN was able to compensate the inhibitory effect of HSA on osteoblast adhesion. Therefore, the presence of lower amounts of coadsorbed albumin may improve presentation of FN in a more integrin-recognized conformation, suggesting that some degree of molecular packing prevents loss of integrin-binding activity. FN reversibility does not seem to be dependent on the HSA/FN adsorption mass ratio in solution, suggesting that FN competitively adsorbs to TiO
                        2 in a favorable conformation and does not suffers subsequent conformational changes allowing exchange with other FN molecules in solution. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {2, 3T3 Cells, Adsorption, Albumins, animal cell, Animals, article, binding kinetics, cell adhesion, Coloring Agents, Competitive protein adsorption, controlled study, cytoskeleton, Data Interpretation, Statistical, Fibronectin, Fibronectins, FN, HSA, human serum albumin, hydrogen peroxide, iodine 125, Iodine Radioisotopes, isotope labeling, Mice, Microscopy, Confocal, mouse, neutral red, nonhuman, osteoblast, Osteoblast adhesion, Osteoblasts, Protein radiolabeling, Proteins, Radiology, Solutions, Sterilization, surface property, TiO, Titanium, Titanium dioxide},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38049002555\&partnerID=40\&md5=dd5a4dee91d3fe1e9eb9a6ba31553c0a},
	author = {Sousa, S.R. and Lamghari, M. and Sampaio, P. and Moradas-Ferreira, P. and Barbosa, M.A.}
}
@article {2354,
	title = {PLD bioactive ceramic films: The influence of CaO-P                    2O 
                    5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells
},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {19},
	year = {2008},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-007-3331-zPubMed ID: 18058197Language of Original Document: EnglishCorrespondence Address: Oliveira, G. M.; Universidade Fernando Pessoa, Pra{\c c}a Nove de Abril 349, 4249-004 Porto, Portugal; email: gisela@ufp.ptChemicals/CAS: calcium oxide, 1305-78-8; hydroxyapatite, 1306-06-5, 51198-94-8; oxide, 16833-27-5; Biocompatible Materials; Calcium Compounds; Ceramics; Durapatite, 1306-06-5; Oxides; Phosphorus Compounds; Polystyrenes; lime, 1305-78-8; phosphorus pentoxide, 1314-56-3References: Hench, L.L., (1991) J. Am. Ceram. Soc., 74, p. 1487;Hench, L.L., (1998) Biomaterials, 19, p. 1419; 
Park, S.H., Llin{\'a}s, A., Goel, V.K., Keller, J.C., (2000) The Biomedical Engineering Handbook, , Edited by: J. D. BRONZINO (CRC Press, LLC, Boca Raton); 
Hench, L.L., (1994) 7th International Symposium on Ceramics in Medicine, p. 3. , Edited by: {\"O}. H. A. A. Y. URPO (Butterworth-Heinemann Ltd Oxford, England); 
Kokubo, T., (1991) Biomaterials, 12, p. 155; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, p. 6. , JAAS Takayan Press. Ishiyaku euroAmerica, Inc Tokyo; 
Orlovskii, V.P., Komlev, V.S., Barinov, S.M., (2002) Inorg. Mater., 38, p. 973; 
Kenny, S.M., Buggy, M., (2003) J. Mater. Sci. Mat. Med., 14, p. 923; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., (1995) J. Mat. Sci. Mat. Med., 6, p. 348; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., (2001) J. Mater. Sci.: Mater. Med., 12, p. 629; 
Ferraz, M.P., Monteiro, F.J., Gi{\~a}o, D., L{\'e}on, B., Gonzalez, P., Liste, S., Serra, J., Perez-Amor, M., (2004) Key Eng. Mat., 254-256, p. 347; 
Porter, A.E., Hobbs, L.W., Benezra Rosen, V., Spector, M., (2002) Biomaterials, 23, p. 725; 
Kim, H.-K., Jang, J.-W., Lee, C.-H., (2004) J. Mater. Sci.: Mater. Med., 15, p. 825; 
Harada, Y., Wang, J.-T., Doppalapudi, V.A., Willis, A.A., Jasty, M., Harris, W.H., Nagase, M., Goldring, S.R., (1996) J. Biomed. Mater. Res., 31, p. 19; 
Arias, J.L., Mayor, M.B., Pou, J., L{\'e}on, B., P{\'e}rez-Amor, M., (2000) Appl. Surf. Sci., 154-155, p. 434; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., (1996) J. Mater. Sci.: Mater. Med., 7, p. 187; 
Arias, J.L., Mayor, M.B., Pou, J., Leng, Y., L{\'e}on, B., P{\'e}rez-Amor, M., (2003) Biomaterials, 24, p. 3403; 
Arias, J.L., Mayor, M.B., Pou, J., L{\'e}on, B., P{\'e}rez-Amor, M., (2002) Appl. Surf. Sci., 182, p. 448; 
Arias, J.L., Mayor, M.B., Pou, J., L{\'e}on, B., P{\'e}rez-Amor, M., (2002) Vaccum, 67, p. 653; 
Arias, J.L., Garcia-Sanz, F.J., Mayor, M.B., Chiussi, S., Pou, J., L{\'e}on, B., P{\'e}rez-Amor, M., (1998) Biomaterials, 19, p. 883; 
Mayor, M.B., Arias, J.L., Chiussi, S., Garcia, F., Pou, J., L{\'e}on, B., P{\'e}rez-Amor, M., (1998) Thin Solid Films, 317, p. 363; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsuki, T., Yamamuro, T., (1990) J. Biomed. Mater. Res., 24, p. 721; 
Monteiro, F.J., Ferraz, M.P., Gi{\~a}o, D., Leon, B., Gonzalez, P., Liste, S., Serra, J., Perez-Amor, M., (2002) 17th European Conference on Biomaterials, pp. P65. , Edited by: E. S. F. Biomaterials (Barcelona, Spain); 
Lossd{\"o}rfer, S., Schwartz, Z., Wang, L., Lohmann, C.H., Turner, J.D., Wieland, M., Cochran, D.L., Boyan, B.D., (2004) J. Biomed. Mater. Res, 70, p. 361; 
Bannister, S.R., Lohmann, C.H., Liu, Y., Sylvia, V.L., Cochran, D.L., Dean, D.D., Boyan, B.D., Schwartz, Z., (2002) J. Biomed. Mater. Res., 60, p. 164; 
Cleries, L., Fernandez-Pradas, J.M., Sardin, G., Morenza, J.L., (1998) Biomaterials, 19, p. 1483; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., (1996) J. Mater. Sci.: Mater. Med., 7, p. 21; 
Knabe, C., Klar, F., Fitzner, R., Radlanski, R.J., Gross, U., (2002) Biomaterials, 23, p. 3235; 
Garcia, F., Arias, J.L., Mayor, M.B., Pou, J., Rehman, I., Knowles, J., Best, S., Bonefield, W., (1998) J. Biomed. Mater. Res. (Appl. Biomater), 43, p. 69; 
Peng, P., Kumar, S., Voelcker, N.H., Szili, E., Smart, R.S.C., Griesser, H.J., (2006) J. Biomed. Mater. Res., 76, p. 347; 
Borsari, V., Giavaresi, G., Fini, M., Torriceli, P., Salito, A., Chiesa, R., Chiusoli, L., Giardino, R., (2005) J. Biomed. Mater. Res. Part B: Appl. Biomater., 75, p. 359; 
Massaro, C., Baker, M.A., Cosentino, F., Ramires, P.A., Klose, S., Milella, E., (2001) J. Biomed. Mater. Res., 58, p. 651; 
Bigi, A., Bracci, B., Cuisinier, F., Elkaim, R., Fini, M., Mayer, I., Mihailescu, I.N., Torricelli, P., (2005) Biomaterials, 26, p. 2381; 
Zeng, H., Lacefield, W.R., (2000) J. Biomed. Mater. Res., 50, p. 239; 
Cl{\`e}ries, L., Fernandez-Pradas, J.M., Morenza, J.L., (2000) Biomaterials, 21, p. 1861; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res., 45, p. 376; 
Li, P., Ducheyne, P., (1998) J. Biomed. Mater. Res., 41, p. 341; 
Weng, J., Liu, Q., Wolke, J.G., Zhang, X., De Groot, K., (1997) Biomaterials, 18, p. 1027; 
Tas, A.C., (2000) Biomaterials, 21, p. 1429; 
Kokubo, T., Kim, H.-M., Kawashita, M., (2003) Biomaterials, 24, p. 2161; 
Dewez, J.-L., Doren, A., Schneider, Y.-J., Rouxhet, P.G., (1999) Biomaterials, 20, p. 547; 
Anselme, K., Linez, P., Bigerelle, M., Le Maguer, D., Le Maguer, A., Hardouin, P., Hildebrand, H.F., Leroy, J.M., (2000) Biomaterials, 21, p. 1567; 
Okumura, A., Goto, M., Goto, T., Yoshinari, M., Masuko, S., Katsuki, T., Tanaka, T., (2001) Biomaterials, 22, p. 2263; 
Kim, H.J., Kim, S.H., Kim, M.S., Lee, E.J., Oh, H.G., Oh, W.M., Park, S.W., Dean, D.D., (2005) J. Biomed. Mater. Res., 74, p. 366
},
	month = {2008///},
	pages = {1775 - 1785},
	abstract = {This work consists on the evaluation of the in vitro performance of Ti6Al4V samples PLD (pulsed laser deposition) coated with hydroxyapatite, both pure and mixed with a CaO-P                        2O
                        5 glass. Previous studies on immersion of PLD coatings in SBF, showed that the immersion apatite films did not present the usual cauliflower morphology but replicated the original columnar structure and exhibited good bioactivity. However, the influence of glass associated to hydroxyapatite concerning adhesion, proliferation and morphology of MG63 cells on the films surface was unclear. In this study, the performance of these PLD coated samples was evaluated, not only following the physical-chemical transformations resulting from the SBF immersion, but also evaluating the cytocompatibility in contact with osteoblast-like MG63 cells. SEM and AFM confirmed that the bioactive ceramic PLD films reproduce the substrate{\textquoteright}s surface topography and that the films presented good adherence and uniform surface roughness. Physical-chemical phenomena occurring during immersion in SBF did not modify the original columnar structure. In contact with MG63 cells, coated samples exhibited very good acceptance and cytocompatibility when compared to control. The glass mixed with hydroxyapatite induced higher cellular proliferation. Cells grown on these samples presented many filipodia and granular structures, typical features of osteoblasts. {\textcopyright} 2007 Springer Science+Business Media, LLC.
},
	keywords = {Apatite, Apatite films, Bioactive ceramic films, Bioactive glass, Bioactivity, Bioceramics, Biocompatible Materials, Biofilms, Brassica oleracea var. botrytis, Calcium Compounds, calcium oxide, Cauliflower morphology, cell adhesion, cell proliferation, cell structure, cell survival, ceramics, Columnar structure, conference paper, controlled study, Durapatite, glass, human, human cell, Humans, hydroxyapatite, Lasers, materials testing, Microscopy, Atomic Force, Microscopy, Electron, Scanning, morphology, osteoblast, Osteoblasts, oxide, Oxides, phosphorous pentoxide, Phosphorus Compounds, Polystyrenes, priority journal, Pulsed laser deposition, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-40949140642\&partnerID=40\&md5=de09d5133a0616668cb5e57135d91ae6},
	author = {Oliveira, G.M. and Ferraz, M.P. and Gonz{\'a}lez, P.G. and Serra, J. and Leon, B. and P{\`e}rez-Amor, M. and Monteiro, F.J.}
}
@book {2344,
	title = {Region and graph-based motion segmentation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5112 LNCS},
	year = {2008},
	note = {Conference code: 72695Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-540-69812-8_60Language of Original Document: EnglishCorrespondence Address: Monteiro, F. C.; INEB - Instituto de Engenharia Biom{\'e}dicaPortugalReferences: Ayer, S., Sawhney, U.S.: Layered representation of motion video using robust maximum-likelihood estimation of mixture models and mdl encoding. In: Proc. IEEE International Conference on Computer Vision, June 1995, pp. 777-784 (1995)Brox, T., (2005) From pixels to regions: Partial differential equation in image analysis, , PhD thesis, Department of Mathematics and Computer Science, Saarland University, Germany;Brox, T., Bruhn, A., Papenberg, N., Weickert, J.: High Accuracy Optical Flow Estimation Based on a, Theory for Warping. In: Pajdla, T., Matas, J(G.) (eds.) ECCV 2004. LNCS, 3024, pp. 25-36. Springer, Heidelberg (2004)Bruhn, A., Weickert, J., Schn{\"o}rr, C., Lucas/Kanade meets Horn/ Schunck: Combining local and global optic flow methods (2005) International Journal of Computer Vision, 61 (3), pp. 1-21; 
Chang, S.-F., Sikora, T., Puri, A., Overview of the MPEG-7 standard (2001) IEEE Transactions on Circuits and Systems for Video Technology, 11 (6), pp. 688-695; 
Horn, B.K.P., Schunck, B.G., Determining optical flow (1981) Artificial Intelligence, 17 (1-3), pp. 185-203; 
Monteiro, F.C., (2007) Region-based spatial and temporal image segmentation, , PhD thesis, Faculdade de Engenharia da Universidade do Porto, Portugal; 
Monteiro, F.C., Campilho, A.: Spectral Methods in Image Segmentation: A Combined Approach. In: Marques, J.S., P{\'e}rez de la Blanca, N., Pina, P. (eds.) IbPRIA 2005. LNCS, 3523, pp. 191-198. Springer, Heidelberg (2005)MPEG4. MPEG-4 video verification model, version 15.0. ISO/IEC/JTC1/SC29/WG11 N3093 1999Papenberg, N., Bruhn, A., Brox, T., Didas, S., Weickert, J., Highly accurate optic flow computation with theoretically justified warping (2006) Int. Journal of Computer Vision, 67 (2), pp. 141-158; 
Shi, J., Malik, J., Motion segmentation and tracking using normalized cuts (1998) Proc. of IEEE Int. Conference on Computer Vision, pp. 1154-1160; 
Smith, P., (2001) Edge-based motion segmentation, , PhD thesis, Department of Engineering, University of Cambridge; 
Vasconcelos, N., Lippman, A., Empirical bayesian motion segmentation (2001) IEEE Transactions on Pattern Analysis and Machine Intelligence, 23 (2), pp. 217-221; 
Wang, J., Adelson, E., Representing moving images with layers (1994) IEEE Transactions on Image Processing, 3 (5), pp. 625-638; 
Weiss, Y., Adelson, E.H., A unified mixture framework for motion segmentation: Incorporating spatial coherence and estimating the number of models (1996) Proc. of IEEE International Conference on Computer Vision and Pattern Recognition, pp. 321-326. , San Francisco, USA, June
},
	month = {2008///},
	pages = {609 - 618},
	address = {Povoa de Varzim},
	abstract = {This paper describes an approach for integrating motion estimation and region clustering techniques with the purpose of obtaining precise multiple motion segmentation. Motivated by the good results obtained in static segmentation we propose a hybrid approach where motion segmentation is achieved within a region-based clustering approach taken the initial result of a spatial pre-segmentation and extended to include motion information. Motion vectors are first estimated with a multiscale variational method applied directly over the input images and then refined by incorporating segmentation results into a region-based warping scheme. The complete algorithm facilitates obtaining spatially continuous segmentation maps which are closely related to actual object boundaries. A comparative study is made with some of the best known motion segmentation algorithms. {\textcopyright} 2008 Springer-Verlag Berlin Heidelberg.},
	keywords = {Clustering approach, Comparative studies, Conformal mapping, Estimation, Flow of solids, Graph-based, Heidelberg (CO), Hybrid approaches, Image analysis, Image segmentation, Imaging techniques, Input Images, International conferences, Maps, Motion estimation, Motion information, Motion segmentation, Motion vector (MV), Multi scaling, Multiple motion segmentation, Object boundaries, Region clustering, Region-based, Segmentation maps, Segmentation results, Static segmentation, Variational methods},
	isbn = {03029743 (ISSN); 3540698116 (ISBN); 9783540698111 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47749121099\&partnerID=40\&md5=99c29c64b01f394f71f2626a8ac6ce1a},
	author = {Monteiro, F.C. and Campilho, A.}
}
@book {2363,
	title = {Three dimensional macroporous calcium phosphate scaffolds for bone tissue engineering},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {361-363 II},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB, Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Rose, F.R., Cyster, L.A., Grant, D.M., Scotchford, C.A., Howdle, S.M., Shakesheff, K.M.K.M., In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel (2004) Biomaterials, 25 (24), pp. 5507-5514;Anthony, C., Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging (2004) Biomaterials, 25 (20), pp. 4947-4954; 
Teixeira, S., Fernandes, H.M., de Boer, J., Ferraz, M.P., Monteiro, F.J., Mesenchymal stem cell proliferation and morphological analysis on interconnected macroporous structures (2007) Key Engineering Materials, 330-332, pp. 1129-1132. , Vols
},
	month = {2008///},
	pages = {947 - 950},
	abstract = {Calcium phosphate ceramics are widely used as bone substitutes since they are biocompatible and bioactive. Having a chemical composition close to natural bone, calcium phosphate ceramics are promising bone substitute materials in orthopaedics, maxillofacial surgery and dentistry. Hydroxyapatite (HA) and tricalcium phosphate (TCP) are the most commonly used calcium phosphates, because their calcium/phosphorus (Ca/P) ratios are close to that of natural bone and they are relatively stable in physiological environment. HA is a major constituent of bone materials and is resorbed after a long time of residence in the body. In this work, highly porous hydroxyapatite scaffolds were produced by polymer replication method and their properties evaluated by Scanning Electron Microscopy (SEM) and micro computerized tomography (μ-CT).},
	keywords = {Biocompatibility, Bone, calcium phosphate, Calcium phosphates, Computerized tomography, Maxillofacial prostheses, Maxillofacial surgery, Micro computerized tomography, Porous, Scaffolds, Scanning electron microscopy, Tissue engineering},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38149045804\&partnerID=40\&md5=d137e6232587d31ba95a0c04c781c90a},
	author = {Teixeira, S. and Oliveira, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@conference {2333,
	title = {Tracking of Arabidopsis thaliana root cells in time-lapse microscopy},
	booktitle = {Proceedings - International Conference on Pattern RecognitionProc. Int. Conf. Pattern Recognit.},
	series = {2008 19th International Conference on Pattern Recognition, ICPR 2008},
	year = {2008},
	note = {Conference code: 81859Export Date: 21 November 2012Source: ScopusArt. No.: 4761746CODEN: PICRELanguage of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526;Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095. , August; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of arabidopsis thaliana root meristem in confocal microscopy (2004) Proc. of the Inter. Conf. on Image Analysis and Recognition, pp. 166-174. , Springer LCNS 3212; 
Harris, C., Stephens, M., A combined corner and edge detector (1988) Proc. of the 4th Alvey Vision Conf.; 
Hartley, R., Zisserman, A., (2003) Multiple View Geometry in Computer Vision, , Cambridge University Press; 
Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res., 119, pp. 85-93; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. on PAMI, 13 (6), pp. 583-598; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., A hybrid approach for cell image segmentation (2008) Proc. of the ICIAR, pp. 739-749. , Springer LCNS 5112; 
Meyer, F., Beucher, S., Morphological segmentation (1990) JVCIR, 1 (1), pp. 21-46. , Sept; 
Mohinder, S., Angus, P., (1993) Kalman Filtering Theory and Practice, , Prentice Hall; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A., Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo (2007) Motion07, pp. 13-13
},
	month = {2008///},
	address = {Tampa, FL},
	abstract = {In vivo observation of cells in the Arabidopsis thaliana root, by time-lapse confocal microscopy, is central to biology research. The research herein described is based on large amount of image data, which must be analyzed to determine the location and state of individual cells. Automating the process of cell tracking is an important step to create tools which will facilitate the analysis of cells{\textquoteright} evolution through time. Here we introduce a confocal tracking system designed in two stages. At the image acquisition stage, we track the area under analysis based on point-to-point correspondences and motion estimation. After image acquisition, we compute cell-to-cell correspondences through time. The final result is a temporal structured information about each cell. {\textcopyright} 2008 IEEE.},
	keywords = {Arabidopsis thaliana, Bioinformatics, Cell tracking, confocal microscopy, Image acquisition, Image data, In-vivo, Individual cells, Motion estimation, Pattern recognition, Point correspondence, Root cells, Structured information, Time-lapse microscopy, Tracking system, Two stage},
	isbn = {10514651 (ISSN); 9781424421756 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77957963663\&partnerID=40\&md5=9794c92c760039ecd3cab6b8a89fa8e3},
	author = {Marcuzzo, M. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@conference {2327,
	title = {Watershed framework to region-based image segmentation},
	booktitle = {Proceedings - International Conference on Pattern RecognitionProc. Int. Conf. Pattern Recognit.},
	series = {2008 19th International Conference on Pattern Recognition, ICPR 2008},
	year = {2008},
	note = {Conference code: 81859Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusArt. No.: 4761587CODEN: PICRELanguage of Original Document: EnglishCorrespondence Address: Monteiro, F. C.; INEB - Instituto de Engenharia Biom{\'e}dicaPortugal; email: monteiro@ipb.ptReferences: Callaghan, R.O., Bull, D., Combined morphologicalspectral unsupervised image segmentation (2005) IEEE Transactions on Image Processing, 14 (1), pp. 49-62;Comaniciu, D., Meer, P., Mean shift: A robust approach toward feature space analysis (2002) IEEE Trans. Pattern Analysis and Machine Intelligence, 24 (5), pp. 603-619; 
Cour, T., Benezit, F., Shi, J., Spectral segmentation with multiscale graph decomposition (2005) Proc. of IEEE Int. Conf. on Computer Vision and Pattern Recognition, 2, pp. 1124-1131. , June; 
De Bock, J., De Smet, P., Philips, W., Image segmentation using watersheds and normalized cuts (2005) Proceedings of SPIE - The International Society for Optical Engineering, 5675, pp. 164-173. , Imaging - Vision Geometry XIII; 
Deng, Y., Manjunath, B., Unsupervised segmentation of color-texture regions in images and video (2001) IEEE Transactions on Pattern Analysis and Machine Intelligence, 23 (8), pp. 800-810; 
Martin, D., Fowlkes, C., Tal, D., Malik, J., A database of human segmented natural images and its application to evaluating segmentation algorithms and measuring ecological statistics (2001) Proc. of IEEE Int. Conference on Computer Vision, 2, pp. 416-423. , http://www.cs.berkeley.edu/projects/vision/grouping/segbench/, online at; 
Monteiro, F., (2007) Region-based Spatial and Temporal Image Segmentation, , PhD thesis, Faculdade de Engenharia da Universidade do Porto, Portugal
},
	month = {2008///},
	address = {Tampa, FL},
	abstract = {This paper proposes a new framework to image segmentation which combines edge- and region-based information with spectral techniques through the morphological algorithm of watersheds. A pre-processing step is used to reduce the spatial resolution without losing important image information. An initial partitioning of the image into primitive regions is set by applying a rainfalling watershed algorithm on the image gradient magnitude. This initial partition is the input to a computationally efficient region segmentation process which produces the final segmentation. The latter process uses a region-based similarity graph representation of the image regions. The experimental results clearly demonstrate the effectiveness of the proposed approach to produce simpler segmentations and to compare favourably with state-of-the-art methods. {\textcopyright} 2008 IEEE.},
	keywords = {Computationally efficient, Edge detection, Graph representation, Image gradients, Image information, Image regions, Image segmentation, Landforms, Pre-processing step, Process use, Rainfalling watershed, Region segmentation, Region-based, Spatial resolution, Spectral techniques, State-of-the-art methods, Watersheds},
	isbn = {10514651 (ISSN); 9781424421756 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77957964931\&partnerID=40\&md5=3c1ab50936b31063f75f5b6337ed3335},
	author = {Monteiro, F.C. and Campilho, A.}
}
@conference {2374,
	title = {Automatic vertebra detection in x-ray images},
	booktitle = {Proceedings of the International Symposium CompIMAGE 2006 - Computational Modelling of Objects Represented in Images: Fundamentals, Methods and ApplicationsProc. Int. Symp. CompIMAGE - Comput. Model. Objects Represent. Images: Fundam., Methods Appl.},
	series = {International Symposium CompIMAGE 2006 - Computational Modelling of Objects Represented in Images: Fundamentals, Methods and Applications},
	year = {2007},
	note = {Conference code: 75470Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Moura, D. C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Sinal e Imagem, Porto, PortugalReferences: Benameur, S., Mignotte, M., Labelle, H., Guise, J.A.D., A hierarchical statistical modeling approach for the unsupervised 3-d biplanar reconstruction of the scoliotic spine (2005) IEEE Trans Biomed Eng, 52 (12), pp. 2041-2057. , December;de Bruijne, M. and M. Nielsen (2004a). Image segmentation by shape particle filtering, Chapter III, pp. 722-725. International Conference on Pattern Recognition. IEEE Computer Society Pressde Bruijne, M., Nielsen, M., Shape particle filtering for image segmentation (2004) Lecture Notes in Computer Science, 3216, pp. 168-175. , of, Chapter I, pp, Springer; 
Ghebreab, S., Smeulders, A., Combining strings and necklaces for interactive threedimensional segmentation of spinal images using an integral deformable spine model (2004) IEEE Trans Biomed Eng, 51 (10), pp. 1821-1829. , October; 
Pomero, V., Mitton, D., Laporte, S., de Guise b, J.A., Skalli, W., Fast accurate stereoradiographic 3d-reconstruction of the spine using a combined geometric and statistic model (2004) Clinical Biomechanics, 19 (3), pp. 240-247. , March; 
Scott, I., Cootes, T., Taylor, C., Shape particle filtering for image segmentation (2003) Lecture Notes in Computer Science, 2732, pp. 258-269. , of, Springer; 
Smyth, P., Taylor, C., Adams, J., Vertebral shape: Automatic measurement with active shape models (1999) Radiology, 211 (2), pp. 571-578; 
Zamora, G., Sari-Sarrafa, H., Long, R., Hierarchical segmentation of vertebrae from x-ray images (2003) Med Imaging: Image Process, 5032, pp. 631-642. , SPIE Press
},
	month = {2007///},
	pages = {307 - 312},
	address = {Coimbra},
	abstract = {In this paper we will describe our experiments with x-ray image analysis for vertebra detection in juvenile/adolescent patients with idiopathic scoliotic spines. We will focus on detecting vertebrae location in a anterior-posterior x-ray image in a fully automatic way. For accomplishing this, we propose a set of techniques for (i) isolating the spine by removing other bone structures (e.g. ribs), (ii) detecting vertebrae location along the spine using an hierarchical and progressive threshold analysis, and (iii) detecting vertebrae lateral boundaries. {\textcopyright} 2007 Taylor \& Francis Group.},
	keywords = {Anterior posteriors, Bone structures, Image analysis, Lateral boundaries, Mammals, Threshold analysis, X ray analysis, X ray apparatus, X-ray images},
	isbn = {9780415433495 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-60749117244\&partnerID=40\&md5=94ba6d9db7e15f538851da273b28d8aa},
	author = {Moura, D.C. and Correia, M.V. and Barbosa, J.G. and Reis, A.M. and Laranjeira, M. and Gomes, E.}
}
@book {2373,
	title = {Detection of lung nodule candidates in chest radiographs},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4478 LNCS},
	year = {2007},
	note = {Conference code: 70962Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pereira, C.S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Sinal e Imagem, Campus FEUP (Faculdade de Engenharia da Universidade do Porto), Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: cmsp@fe.up.ptReferences: Wei, J., Hagihara, Y., Shimizu, A., Kobatake, H., Optimal image feature set for detecting lung nodules on chest X-ray images (2002) CARS 2002;Keserci, B., Yoshida, H., Computerized detection of pulmonary nodules in chest radiographs based on morphological features and wavelet snake model (2002) Medical Image Analysis, 6, pp. 431-447; 
Yoshida, H., Local contralateral subtraction based on bilateral symmetry of lung for reduction of false positives in computerized detection of pulmonary nodules, Biomedical Engineering (2004) IEEE Transactions, 51, pp. 778-789; 
Suzuki, K., Shiraishi, J., Abe, H., MacMahon, H., Doi, K., False-positive reduction in computer-aided diagnostic scheme for detecting nodules in chest radiographs by means of massive training artificial neural network (2005) Academic Radiology, 12, pp. 191-201; 
Schilham, A., van Ginneken, B., Loog, M., Multi-scale Nodule Detection in Chest Radiographs (2003) LNCS, 2878, pp. 602-609. , Ellis, R.E, Peters, T.M, eds, MICCAI 2003, Springer, Heidelberg; 
Schilham, A., van Ginneken, B., Loog, M., A computer-aided diagnosis system for detection of lung nodules in chest radiographs with an evaluation on a public database (2006) Medical Image Analysis, 10, pp. 247-258; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Transactions on Image Processing, 8 (8); 
Shiraishi, J., Katsuragawa, S., Kezoe, S., Matsumoto, T., Kobayashi, T., Komatsu, K., Matsui, M., Doi, K., Development of a Digital Image Database for Chest Radiographs With and Without a Lung Nodule: Receiver Operating Characteristic Analysis of Radiologists{\textquoteright} Detection of Pulmonary Nodules (2000) American Journal of Roentgenology, 174, pp. 71-74; 
Kobatake, H., Murakami, M., Adaptive filter to detect rounded convex regions: Iris filter (1996) Int. Conf. Pattern Recognition, 11, pp. 340-344; 
Wei, J., Hagihara, Y., Kobatake, H.: Detection of cancerous tumors on chest X-ray images - Candidate detection filter and its evaluation, ICIP99, no. 27AP4.2 (1999)Kobatake, H., Wei, J., Yoshinaga, Y., Hagihara, Y., Shimizo, A., Nonlinear Adaptine Convergence Index Filters and Their Characteristics (2000) Int. Conf. Pattern Recognition, pp. 522-525
},
	month = {2007///},
	pages = {170 - 177},
	address = {Girona},
	abstract = {This paper presents an automated method for the selection of a set of lung nodule candidates, which is the first stage of a computeraided diagnosis system for the detection of pulmonary nodules. An innovative operator, called sliding band filter (SBF), is used for enhancing the lung field areas. In order to reduce the influence of the blood vessels near the mediastinum, this filtered image is multiplied by a mask that assigns to each lung field point an a priori probability of belonging to a nodule. The result is further processed with a watershed segmentation method that divides each lung field into a set of non-overlapping areas. Suspicious nodule locations are associated with the regions containing the highest regional maximum values. The proposed method, whose result is an ordered set of lung nodule candidate regions, was evaluated on the 247 images of the JSRT database with very promising results. {\textcopyright} Springer-Verlag Berlin Heidelberg 2007.},
	keywords = {Blood Vessels, Computer aided diagnosis, Database systems, Lung nodule candidates, Non-overlapping areas, Pulmonary diseases, Pulmonary nodules, Radiography, Sliding band filter (SBF)},
	isbn = {03029743 (ISSN); 9783540728481 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38049105791\&partnerID=40\&md5=9af5e55fe146cd0455d2302e5383e14c},
	author = {Pereira, C.S. and Fernandes, H. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2372,
	title = {Evaluation of contrast enhancement filters for lung nodule detection},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4633 LNCS},
	year = {2007},
	note = {Conference code: 70855Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pereira, C.S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Sinal e Imagem, Campus FEUP (Faculdade de Engenharia da Universidade do Porto), Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: cmsp@fe.up.ptReferences: Kobatake, H., Murakami, M., Adaptive filter to detect rounded convex regions: Iris filter (1996) Int. Conf. Pattern Recognition, 2, pp. 340-344;Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Transactions on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H.: Detection of cancerous tumors on chest X-ray images - Candidate detection filter and its evaluation. In: ICIP99, 27AP4.2 (1999)Kobatake, H., Wei, J., Yoshinaga, Y., Hagihara, Y., Shimizo, A., Nonlinear Adaptinc Convergence Index Filters and Their Characteristics (2000) Int. Conf. Pattern Recognition, pp. 522-525; 
Shiraishi, J., Katsuragawa, S., Kezoe, S., Matsumoto, T., Kobayashi, T., Komatsu, K., Matsui, M., Doi, K., Development of a Digital Image Database for Chest Radiographs With and Without a Lung Nodule: Receiver Operating Characteristic Analysis of Radiologists{\textquoteright} Detection of Pulmonary Nodules (2000) American Journal of Roentgenology, 174, pp. 71-74; 
Keserci, B., Yoshida, H., Computerized detection of pulmonary nodules in chest radiographs based on morphological features and wavelet snake model (2002) Medical Image Analysis, 6, pp. 431-447; 
Song, X., Pogue, B., Jiang, S., Doyley, M., Dehghani, H., Tosteson, T., Paulsen, K., Automated region detection based on the contrast-to-noise ratio in near-infrared tomography (2004) Applied Optics, 43 (5), pp. 1053-1062; 
Singh, S., Bovis, K., An evaluation of contrast enhancement techniques for mammographic breast masses (2005) IEEE Transactions On Information Technology in Biomedicine, 9 (1), pp. 109-119; 
Wei, J., Hagihara, Y., Shimizu, A., Kobatake, H., Optimal image feature set for detecting lung nodules on chest X-ray images (2002) CARS; 
Suzuki, K., Shiraishi, J., Abe, H., MacMahon, H., Doi, K., False-positive reduction in computer-aided diagnostic scheme for detecting nodules in chest radiographs by means of massive training artificial neural network (2005) Academic Radiology, 12, pp. 191-201; 
},
	month = {2007///},
	pages = {878 - 888},
	address = {Montreal},
	abstract = {The aim of this paper is to evaluate and compare the performance of three convergence index (CI) filters when applied to the enhancement of chest radiographs, aiming at the detection of lung nodules. One of these filters, the sliding band filter (SBF), is the proposal of an innovative operator, while the other two CI class members, the iris filter (IF) and the adaptive ring filter (ARF), are already known in this application. To demonstrate the adequacy of the new filter for the enhancement of chest x-ray images, we calculated several figures of merit with the goal of comparing (i) the contrast enhancement capability of the filters, and (ii) the behavior of the filters for the detection of lung nodules. The results obtained for 154 images with nodules of the JSRT database show that the SBF outperforms both the IF and ARF. The proposed filter demonstrated to be a promising enhancement method, thus justifying its use in the first stage of a computer-aided diagnosis system for the detection of lung nodules. {\textcopyright} Springer-Verlag Berlin Heidelberg 2007.},
	keywords = {Adaptive systems, Contrast enhancement filters, Database systems, Image segmentation, Iris filter (IF), Medical imaging, Radiography, X rays},
	isbn = {03029743 (ISSN); 9783540742586 (ISBN)},
	author = {Pereira, C.S. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@conference {2371,
	title = {A Gait Analysis Laboratory for rehabilitation of patients with musculoskeletal impairments},
	booktitle = {Proceedings of the International Symposium CompIMAGE 2006 - Computational Modelling of Objects Represented in Images: Fundamentals, Methods and ApplicationsProc. Int. Symp. CompIMAGE - Comput. Model. Objects Represent. Images: Fundam., Methods Appl.},
	series = {International Symposium CompIMAGE 2006 - Computational Modelling of Objects Represented in Images: Fundamentals, Methods and Applications},
	year = {2007},
	note = {Conference code: 75470Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Sousa, D. S. S.; INEGI, Instituto de Engenharia Mecǎnica e Gest{\~a}o Industrial, Porto, PortugalReferences: Baker, R., Gait analysis methods in rehabilitation (2006) Journal of Neuro Engineering and Rehabilitation, 3 (1), p. 4;Cappozzo, A., Delia Croce, U., Human movement analysis using stereophotogrammetry. Part I: Theoretical background (2005) Gait \& Posture, 21, pp. 186-196; 
Chau, T., A review of analytical techniques for gait data. Part 1: Fuzzy, statistical and fractal methods (2001) Gait \& Posture, 13 (1), pp. 49-66; 
Davis, R.B., Reflections on clinical gait analysis (1997) Journal of Electromyography and Kinesiology, 7 (4), pp. 251-257; 
Davis III, R.B., Clinical gait analysis (1988) Engineering in Medicine and Biology Magazine, IEEE, 7 (3), pp. 35-40; 
Figueroa, P.J., Leite, N.J., A flexible software for tracking of markers used in human motion analysis (2003) Computer Methods and Programs in Biomedicine, 72, pp. 155-165; 
Gavrila, D., The visual analysis of human movement: A survey (1999) Computer Vision and Image Understanding, 73 (1), pp. 82-98; 
Merletti, R., Parker, P., (2004) Electromyography: Physiology, engineering and noninvasive applications, , Wiley-IEEE Press; 
(1978) NASA Reference Publication, , NASA, Springfield: NASA; 
Orlin, M., McPoil, T., Plantar pressure assessment (2000) Physical therapy, 80 (4), pp. 399-409; 
Perry, J., The contribution of dynamic electromyography to gait analysis (1998) Monograph 002: Gait analysis in the science of rehabilitation. 1998, , J. DeLisa ed, Washington, DC; 
Pinho, R., Tavares, J., A Movement Tracking Management Model with Kalman Filtering, Global Optimization Techniques and Mahalanobis Distance (2005) ICCMSE 2005 - International Conference of Computational Methods in Sciences and Engineering; Loutraki, 21-26 October 2005, , Korinthos: Greece; 
Pinho, R., Tavares, J., Human Movement Tracking and Analysis with Kalman Filtering and Global Optimization Techniques (2005) ICCB2005 - II International Conference on Computational Bioengineering; Instituto Superior T{\'e}cnico, Lisboa, 14-16 September 2005, , Lisboa: Portugal; 
Rosenbaum, D., Becker, H., Plantar pressure distribution measurements. Technical background and clinical applications (1997) Foot and Ankle Surgery, 3 (1), pp. 1-14; 
Roy, K., Force, pressure and motion measurements in the foot: Current concepts (1988) Clinics in Podiatric Medicine and Surgery, 5, pp. 491-508; 
Schmalz, T., Blumentrit, S., Energy expenditure and biomechanical characteristics of lower limb amputee gait: The influence of prosthetic alignment and different prosthetic components (2002) Gait \& Posture, 16, pp. 255-263; 
Shabana, A., (2001) Computational Dynamics, , NY: John Wiley; 
Sisto, S. 1998. An overview of the value of information resulting from instrumented gait analysis for the physical therapist. In J. DeLisa (ed.) Monograph 002: Gait analysis in the science of rehabilitation. 1998. Washington, DC. Sutherland, D. 2001. The evolution of clinical gait analysis part 1: kinesiological EMG. Gait \& Posture 14(1): 61-70Sutherland, D., The evolution of clinical gait analysis: Part II Kinematics (2002) Gait \& Posture, 16 (2), pp. 159-179; 
Tavares, J., Bastos, L., Improvement of Modal Matching Image Objects in Dynamic Pedobarography using Optimization Techniques (2005) Electronic Letters on Computer Vision and Image Analysis, special issue on Articuled Motion \& Deformable Objects, 5, pp. 1-20; 
Tavares, J., Barbosa, J., Matching Image Objects in Dynamic Pedobarography (2000) RecPad 2000 - 11th Portuguese Conference on Pattern Recognition; Porto, 11-12 Maio 2000, , Porto, Portugal; 
Urry, S., Plantar pressure-measurement sensors (1999) Measurement Science and Technology, 10, pp. 16-32; 
Whittle, M., Clinical gait analysis: A review (1996) Human Movement Science, 15, pp. 369-387; 
Whittle, M., (2003) Gait analysis an introduction, , Oxford, Boston: Butterworth-Heinemann; 
Winter, D., (2005) Biomechanics and motor control of human movement, , Hoboken: John Wiley \& Sons
},
	month = {2007///},
	pages = {417 - 422},
	address = {Coimbra},
	abstract = {This paper describes the technology (software \& hardware) used in the Gait Analysis Laboratory of CRPG - Centro de Reabilita{\c c}{\~a}o Projissional de Gaia. This laboratory is mainly intended to support CRPG with quantitative data for clinical rehabilitation of patients with musculoskeletal pathologies, namely in the lower limbs. Usually, for the gait analysis is considered data obtained from several acquisition sensors, such as: image cameras, electromyographic devices, pressure devices and force plates. This paper also presents briefly how quantitative motion analysis can be used for a clinical point of view and it presents the influence of image algorithms in gait rehabilitation. {\textcopyright} 2007 Taylor \& Francis Group.},
	keywords = {Acquisition sensors, Electromyographic, Force plates, Gait analysis, Gait rehabilitations, Image algorithms, Lower limbs, Motion analysis, Patient rehabilitation, Pressure devices, Quantitative datum},
	isbn = {9780415433495 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-60749114216\&partnerID=40\&md5=097e0d95d00419c881b3139811e93686},
	author = {Sousa, D.S.S. and Tavares, J.M.R.S. and Correia, M.V. and Barbosa, J.G. and Ch{\~a}, C.V. and Mendes, E.}
}
@article {2378,
	title = {Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {81},
	year = {2007},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31073PubMed ID: 17252543Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Ceramics; Durapatite, 1306-06-5Manufacturers: Plasma BiotalReferences: Teixeira S, Queiroz AC, Monteiro FJ, Ferraz MP, Vilar R, Eugenio S. Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results. Bioceramics 2006;18 (Parts 1, 2):309-311, 105-108Fernandez-Pradas, J.M., Cleries, L., Martinez, E., Sardin, G., Esteve, J., Morenza, J.L., Influence of thickness on the properties of hydroxyapatite coatings deposited by KrF laser ablation (2001) Biomaterials, 22, pp. 2171-2175;Fernandez-Pradas, J.M., Cleries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the deposition rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys A: Mater Sci Process, 72, pp. 613-618; 
Foltyn, S.R., Dye, R., Ott, K.C., Peterson, E., Hubbard, K.M., Hutchinson, W., Muenchausen, R.E., Wu, X.D., Target modification in the excimer laser deposition of YBa 2Cu 3O 7-x thin films (1991) Appl Phys Lett, 59, pp. 594-596; 
Khor, K.A., Vreeling, A., Dong, Z.L., Cheang, P., Laser treatment of plasma sprayed HA coatings (1999) Mater Sci Eng A: Struct Mater Prop Microstruct Process, 266 (1-2), pp. 1-7; 
Ricci, J.L., Alexander, H., Laser microtexturing of implant surfaces for enhanced tissue integration (2001) Key Engineering Mater, pp. 198-199,179-202; 
Galli, C., Guizzardi, S., Passeri, G., Martini, D., Tinti, A., Mauro, G., Macaluso, G.M., Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces (2005) J Periodontol, 76, pp. 364-372; 
Oliveira, V., Conde, O., Vilar, R., UV laser micromachining of ceramic materials: Formation of columnar topographies (2001) Adv Eng Mater, 3 (1-2), pp. 75-81; 
Serra, P., FernandezPradas, J., Sardin, G., Morenza, J.L., Interaction effects of an excimer laser beam with hydroxyapatite targets (1997) Appl Surf Sci, 110, pp. 384-388; 
Serra, P., Morenza, J.L., Fluence dependence of hydroxyapatite laser ablation plumes (1998) Thin Solid Films, 335 (1-2), pp. 43-48; 
Duncan, A.C., Weisbuch, F., Rouais, F., Lazare, S., Baquey, C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosens Bioelectron, 17, pp. 413-426; 
Walboomers, X.F., Croes, H.J.E., Ginsel, L.A., Jansen, J.A., Contact guidance of rat fibroblasts on various implant materials (1999) J Biomed Mater Res, 47, pp. 204-212; 
Carvalho, P., Manzanares, M.C., Franch, J., Belmonte, A.M., Lafuente, M.P., Serra, I., Fernandez-Pradas, J.M., Morenza, J.L., Osteointegration of calcium phosphate coatings obtained by pulsed laser deposition (2003) J Dent Res, 82, p. 573; 
Ahmad, M., McCarthy, M., Gronowicz, G., An in vitro model for mineralization of human osteoblast-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220; 
Matsuura, T., Hosokawa, R., Okamoto, K., Kimoto, T., Akagawa, Y., Diverse mechanisms of osteoblast spreading on hydroxyapatite and titanium (2000) Biomaterials, 21, pp. 1121-1127; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Giardino, R., Comparative interspecies investigation on osteoblast cultures: Data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57, pp. 57-62; 
Boyan, B.D., Lossdoerfer, S., Wang, L., Zhao, G., Lohmann, C.H., Cochran, D.L., Schwartz, Z., Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies (2003) Eur Cell Mater, 6, pp. 22-27; 
Anselme, K., Bigerelle, M., Topography effects of pure titanium substrates on human osteoblast long-term adhesion (2005) Acta Biomater, 1, pp. 211-222; 
Anselme, K., Linez, P., Bigerelle, M., Le Maguer, D., Le Maguer, A., Hardouin, P., Hildebrand, H.F., Leroy, J.M., The relative influence of the topography and chemistry of TiAl 6V 4 surfaces on osteoblastic cell behaviour (2000) Biomaterials, 21, pp. 1567-1577; 
Anselme, K., Noel, B., Hardouin, P., Human osteoblast adhesion on titanium alloy, stainless steel, glass and plastic substrates with same surface topography (1999) J Mater Sci: Mater Med, 10, pp. 815-819; 
Cleries L, Fernandez-Pradas JM, Morenza JL. Bone growth on and resorption of calcium phosphate coatings obtained by pulsed laser deposition. J Biomed Mater Res 2000;49:43. Erratum 2001;57:473Deligianni, D.D., Katsala, N., Ladas, S., Sotiropoulou, D., Amedee, J., Missirlis, Y.F., Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption (2001) Biomaterials, 22, pp. 1241-1251; 
dos Santos, E.A., Farina, M., Soares, G.A., Specific proliferation rates of human osteoblasts on calcium phosphate surfaces with variable concentrations of α-TCP (2007) Mater Sci Eng, 27, pp. 61-66; 
Hao, L., Lawrence, J., Laser surface treatment of magnesia partially stabilized zirconia for enhanced human skin fibroblast cell response (2004) J Laser Appl, 16, pp. 55-64; 
Kieswetter, K., Schwartz, Z., Dean, D.D., Boyan, B.D., The role of implant surface characteristics in the healing of bone (1996) Crit Rev Oral Biol Med, 7, pp. 329-345; 
Salda{\~n}a, L., Gonz{\'n}lez-Carrasco, J.L., Rodriguez, M., Munuera, L., Vilaboa, N., Osteoblast response to plasma-spray porous Ti 6AL 4V coating on substrates of identical alloy (2006) J Biomed Mater Res A, 77, pp. 608-617; 
Lincks, J., Boyan, B.D., Blanchard, C.R., Lohmann, C.H., Liu, Y., Cochran, D.L., Dean, D.D., Schwartz, Z., Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition (1998) Biomaterials, 19, pp. 2219-2232; 
Martin, J.Y., Dean, D.D., Cochran, D.L., Simpson, J., Boyan, B.D., Schwartz, Z., Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces (1996) Clin Oral Implants Res, 7, pp. 27-37; 
Noth, U., Hendrich, C., Merklein, F., Altvater, T., Rader, C.P., Schutze, N., Eulert, J., Thull, R., Standardised testing of bone-implant surfaces using an osteoblast cell culture system. II. Titanium surfaces of varying degrees of roughness (1999) Biomedizinische Technik, 44 (1-2), pp. 6-11; 
Ponsonnet, L., Reybier, K., Jaffrezic, N., Comte, V., Lagneau, C., Lissac, M., Martelet, C., Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour (2003) Mater Sci Eng C: Biomimetic Supramolecular Syst, 23, pp. 551-560; 
Ramires, P.A., Romito, A., Cosentino, F., Milella, E., The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour (2001) Biomaterials, 22, pp. 1467-1474; 
Redey, S.A., Nardin, M., Bernache-Assolant, D., Rey, C., Delannoy, P., Sedel, L., Marie, P.J., Behavior of human osteoblastic cells on stoichiometric hydroxyapatite and type A carbonate apatite: Role of surface energy (2000) J Biomed Mater Res, 50, pp. 353-364; 
Rosa, A.L., Beloti, M.M., van Noort, R., Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography (2003) Dent Mater, 19, pp. 768-772; 
Schmidt, C., Ignatius, A.A., Claes, L.E., Proliferation and differentiation parameters of human osteoblasts on titanium and steel surfaces (2001) J Biomed Mater Res, 54, pp. 209-215; 
Schwartz, Z., Kieswetter, K., Dean, D.D., Boyan, B.D., Underlying mechanisms at the bone-surface interface during regeneration (1997) J Periodontal Res, 32, pp. 166-171; 
Schwartz, Z., Martin, J.Y., Dean, D.D., Simpson, J., Cochran, D.L., Boyan, B.D., Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation (1996) J Biomed Mater Res, 30, pp. 145-155; 
Wang, J., de Boer, J., de Groot, K., Preparation and characterization of electrodeposited calcium phosphate/chitosan coating on Ti 6Al 4V plates (2004) J Dent Res, 83, pp. 296-301; 
Xavier, S.P., Carvalho, P.S.P., Beloti, M.M., Rosa, A.L., Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments (2003) J Dent, 31, pp. 173-180
},
	month = {2007///},
	pages = {920 - 929},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture, and microchemistry. The surface topography eventually affects the nature and the intensity of the interactions that occur at biomaterial- biological interface (cell adhesion, mobility, spreading, and proliferation). Therefore, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (in this case, osteoblasts). There are a number of substrates that already have been studied in terms of surface topography; however, few studies are related to hydroxyapatite (HA) substrates. As it is well established, HA is a well-known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense HA substrates was altered by using KFr excimer laser. The surface was characterized by atomic force microscopy and contact angle measurements, while the cell distribution and morphology was assessed by scanning electron microscopy and confocal laser scanning microscopy. Results revealed that the surface is characterized by a homogeneous columnar structure with high specific area. Moreover, cells were able to attach and spread on the surface of the samples, and gradually grow into nearly confluent monolayers. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {article, Atomic force microscopy, Biocompatibility, biomaterial, cell adhesion, Cell Communication, Cell Line, Tumor, cell motility, cell proliferation, cell spreading, cellular distribution, ceramic prosthesis, ceramics, chemistry, confocal laser microscopy, controlled study, Durapatite, Excimer laser, Excimer lasers, human, human cell, Humans, hydroxyapatite, laser, Lasers, material coating, Microchemistry, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Electron, Scanning, Microtexture, monolayer culture, osteoblast, Osteoblasts, reproducibility, Scanning electron microscopy, Surface microtopography, Surface modification, Surface Properties, surface property, Surface topography, Surface treatment, Tissue engineering},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249742544\&partnerID=40\&md5=b6cb0aaa7b8e4a267b97d91d7171862f},
	author = {Teixeira, S. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@book {2383,
	title = {Mesenchymal stem cell proliferation and morphological analysis on interconnected macroporous structures},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 II},
	year = {2007},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre,823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Mastrogiacomo, M., (2006) Biomaterials, 27, p. 3230;Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Materials, pp. 455,358. , Science Forum; 
Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Key Engineering Materials, pp. 254,997; 
Moroni, L., (2006) Biomaterials, 27, p. 4911
},
	month = {2007///},
	pages = {1129 - 1132},
	abstract = {Calcium phosphate ceramics are widely used as bone substitutes since they are biocompatible and bioactive. Given that their chemical composition is close to natural bone, calcium phosphate ceramics are promising bone substitute materials in orthopaedics, maxillofacial surgery and dentistry. Hydroxyapatite (HA) and tricalcium phosphate (TCP) are the most commonly used calcium phosphates, because their calcium/phosphorus (Ca/P) ratios are close to that of natural bone and they are relatively stable in physiological environment. Furthermore, other critical parameters must be accomplished when designing a biomaterial for bone regeneration, namely: pore size, shape and interconnectivity [1]. Porosity is one of the most important factors since it influences the adhesion, migration nutrient supply and ultimately, proliferation of mesenchymal stem cells. In this study, HA scaffolds with controlled porosity were obtained and their capacity to support human and rat mesenchymal stem cells attachment and proliferation was evaluated.},
	keywords = {Biomaterials, Calcium Compounds, cell adhesion, cytology, hydroxyapatite, Macroporous ceramics, Mesenchymal Stem Cells, orthopedics, Phase composition, Physiological models, porosity, Tricalcium phosphate (TCP)},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846317063\&partnerID=40\&md5=ba9f628d2ad403a6ab70a617a1c9b273},
	author = {Teixeira, S. and Fernandes, H.M. and De Boer, J. and Ferraz, M.P. and Monteiro, F.J.}
}
@book {2384,
	title = {Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 I},
	year = {2007},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Pataquiva Mateus, A.Y.; INEB - Institute de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptReferences: Liou, S., Chen, S., Liu, D., (2003) Biomaterials, 24, p. 3981;Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., (1999) Journal of Biomedical Materials Research, 47, p. 28; 
Grimaldi, G., Weiss, P., Millot, F., Daculsi, G., (1998) Journal of Biomedical Materials Research, 39, p. 660; 
Termenoff, J., Mikos, A., (2000) Biomaterials, 21, p. 2405; 
Best, S., Bonfield, W., Doyle, C., (1989) Bioceramics, p. 68. , Oonishi H, Aoki H, Sawai K, editors, Tokyo: Ishiyaku Euro-America; 
Pataquiva-Mateus, A.Y., Ferraz, M.P., Monteiro, F.J., Journal of Biomedical Materials Research, , Submitted paper
},
	month = {2007///},
	pages = {243 - 246},
	abstract = {This study concerns the preparation and characterisation of microspheres associating alginate and two different types of hydroxyapatite (HA), which are intended to be used as drug delivery systems and bone regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using a chemical precipitation synthesis based on H3PO4, Ca(OH)2 and a surfactant, SDS (sodium dodecylsulphate), as starting reagents. These two powders of nanoHA and alginate were used to prepare two different types of microspheres. Both powders and microspheres were characterised using FTIR, TEM, SEM, mercury porosimetry analysis and X-ray diffraction Results show that pure hydroxyapatite (HA) and mixtures of HA/β-TCP in the nanometre range were obtained from both HA syntheses. Microspheres with different characteristics were obtained from these two types of hydroxyapatite.},
	keywords = {Alginate, Bone, Bone Regeneration, Chemical precipitation, Controlled drug delivery, Fourier transform infrared spectroscopy, hydroxyapatite, Microspheres, Microstructure, nanoparticles, Nanostructured materials, Scanning electron microscopy, Surface active agents, transmission electron microscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846333853\&partnerID=40\&md5=f694c3d331dea2c82aba11cef4dddd59},
	author = {Pataquiva Mateus, A.Y. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2377,
	title = {Nanohydroxyapatite microspheres as delivery system for antibiotics: Release kinetics, antimicrobial activity, and interaction with osteoblasts},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {81},
	year = {2007},
	note = {Cited By (since 1996): 32Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31151PubMed ID: 17252559Language of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mpferraz@ufp.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; amoxicillin plus clavulanic acid, 74469-00-4, 79198-29-1; clindamycin, 18323-44-9; erythromycin, 114-07-8, 70536-18-4; hydroxyapatite, 1306-06-5, 51198-94-8; metronidazole, 39322-38-8, 443-48-1; minocycline, 10118-90-8, 11006-27-2, 13614-98-7; tetracycline, 23843-90-5, 60-54-8, 64-75-5; Anti-Bacterial Agents; Durapatite, 1306-06-5References: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical-evaluation of porous and nonporous hydroxyapatite in the treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528;Kinane, D.F., Lindhe, J., Pathogenesis of periodontitis (2002) Clinical Periodontology and Implant Dentistry, pp. 189-225. , Lindhe J, Karring T, Lang NP, editors, Copenhagen: Munksgaard;; 
Slots, J., Armitage, G.C., Cochran, D., Cohen, R.E., Greenstein, G., Mariotti, A., Rethman, M.P., and others, Systemic antibiotics in periodontics (1996) J Periodontol, 67, pp. 831-838; 
Mombelli, A., Antibiotics in periodontal therapy (2002) Clinical Periodontology and Implant Dentistry, , Lindhe J, Karring T, Lang NP, editors, Copenhagen: Munksgaard;; 
Etienne, D., Locally delivered antimicrobials for the treatment of chronic periodontitis (2003) Oral Dis, 9, pp. 45-50; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Porous HA scaffolds for drug releasing (2005) Bioceramics, 17, pp. 407-410; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Porous hydroxyapatite and glass reinforced hydroxyapatite for controlled release of sodium ampicillin (2004) Bioceramics, 16, pp. 997-1000; 
Hannes, P.J., Purvis, J.P., Local anti-infective therapy: Pharmacological agents. A systemic review (2003) Ann Periodontol, 8, pp. 79-98; 
Soskolne, W.A., Heasman, P.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis: A multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Greenstein, G., Polson, A., The role of local drug delivery in the management of periodontal diseases: A comprehensive review (1998) J Periodontol, 69, pp. 507-520; 
Killoy, W.J., Chemical treatment of periodontitis: Local delivery of antimicrobials (1998) Int Dent J, 48, pp. 305-315; 
Schwach-Abdellaoui, K., Vivien-Castioni, N., Gurny, R., Local delivery of antimicrobial agents for the treatment of periodontal diseases (2000) Eur J Pharm Biopharm, 50, pp. 83-99; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Development of a system to adsorb drugs onto calcium phosphate materials (2005) J Mater Sci-Mater Med, 16, pp. 641-646; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Adsorption isotherm of sodium ampicillin onto dense and porous hydroxyapatite (2005) Bioceramics, 17, pp. 387-390; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., da Silva, MHP dissolution studies of hydroxyapatite and glass-reinforced hydroxyapatite ceramics (2003) Mater Char, 50, pp. 197-202; 
Rauschmann, M.A., Wichelhaus, T.A., Stirnal, V., Dingeldein, E., Zichner, L., Schnettler, R., Alt, V., Nanocrystalline hydroxyapatite and calcium sulphate as biodegradable composite carrier material for local delivery of antibiotics in bone infections (2005) Biomaterials, 26, pp. 2677-2684; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Production of porus hydroxyapatite with potential for controlled drug delivery (2004) Mater Sci Forum, 455-456, pp. 358-360; 
Daculsi, G., Passuti, N., Effect of the macroporosity for osseous substitution of calcium-phosphate ceramics (1990) Biomaterials, 11, pp. 86-87; 
Gauthier, O., Bouler, J.M., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics (2000) J Biomed Mater Res, 51, pp. 475-483; 
Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., Enhanced functions of osteoblasts on nanophase ceramics (2000) Biomaterials, 21, pp. 1803-1810; 
Webster, T.J., Siegel, R.W., Bizios, R., Enhanced surface and mechanical properties of nanophase ceramics to achieve orthopaedic/dental implant efficacy (2000) Bioceramics, 13, pp. 321-324; 
Liou, S.C., Chen, S.Y., Liu, D.M., Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios (2003) Biomaterials, 24, pp. 3981-3988; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.D., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80; 
Barrias CC, Ribeiro CC, Martins MCL, Barbosa MA, Rodrigues D, Miranda MCS. Calcium phosphate microspheres for localised delivery of a therapeutic enzyme. Bioceramics 2006;18 (Parts 1/2):903-906Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Socransky, S.S., Haffajee, A.D., Microbiology of periodontal disease (2002) Clinical Periodontology and Implant Dentistry, , Lindhe J, editor, Copenhagen: Munksgaard;; 
Slots, J., Selection of antimicrobial agents in periodontal therapy (2002) J Periodontal Res, 37, pp. 389-398; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J Mater Sci-Mater Med, 17, pp. 455-463; 
Barrias, C.C., Ribeiro, C.C., Lamghari, M., S{\'a}, C., Barbosa, M.A., Proliferation, activity and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) J Biomed Mater Res A, 72, pp. 57-66; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Comparative study of injectable material synthesised from alginate and two types of hydroxyapatite Submitted for publicationFerraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P2O5 glass hydroxyapatite double layer plasma sprayed coatings: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Nakamura, T., Kitsogi, T., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J Biomed Mater Res, 24, pp. 471-488; 
Gautier, H., Daculsi, G., Merle, C., Association of vancomycin and calcium phosphate by dynamic compaction: In vitro characterization and microbiological activity (2001) Biomaterials, 22, pp. 2481-2487; 
Kimakhe, S., Bohic, S., Larrose, C., Reynaud, A., Pilet, P., Giumelli, B., Heymann, D., Daculsi, G., Biological activities of sustained polymyxin B release from calcium phosphate biomaterial prepared by dynamic compaction: An in vitro study (1999) J Biomed Mater Res, 47, pp. 18-27; 
Korkusuz, F., Uchida, A., Shinto, Y., Araki, N., Inoue, K., Ono, K., Experimental implant-related osteomyelitis treated by antibiotic-calcium hydroxyapatite ceramic composites (1993) J Bone Joint Surg-Br, 75, pp. 111-114; 
Otsuka, M., Matsuda, Y., Kokubo, T., Yoshihara, S., Nakamura, T., Yamamuro, T., A novel skeletal drug-delivery system using self-setting bioactive glass bone-cement. II. Drug-release from a novel self-setting bioactive glass bone-cement containing cephalexin and its physicochemical properties (1995) J Biomed Mater Res, 29, pp. 33-38; 
Shinto, Y., Uchida, A., Korkusuz, F., Araki, N., Ono, K., Calcium hydroxyapatite ceramic used as a delivery system for antibiotics (1992) J Bone Joint Surg Br, 74, pp. 600-604; 
Yamamura, K., Iwata, H., Yotsuyanagi, T., Synthesis of antibiotic-loaded hydroxyapatite beads and invitro drug release testing (1992) J Biomed Mater Res, 26, pp. 1053-1064; 
Clover, J., Gowen, M., Are Mg-63 and Hos Te85 human osteosarcoma cell-lines representative models of the osteoblastic phenotype? (1994) Bone, 15, pp. 585-591
},
	month = {2007///},
	pages = {994 - 1004},
	abstract = {Severe periodontitis treatment, where massive alveolar bone loss occurs, involves bone defect filling and intensive systemic log-term antibiotics administration. This study aims at developing novel injectable drug delivery systems (nanohydroxyapatite microspheres) with the drug releasing capability for periodontitis treatment and simultaneously initiating the osteointegration process. Materials were characterized by XRD, SEM, inverted stand optical microscope analysis, and mercury porosimetry method. Amoxicillin, amoxicillin + clavulanic acid, and erythromycin were the antibiotics used. Release properties during 28 days from the hydroxyapatite (HA) granules, and two types of nanoHA microspheres were investigated. Biocompatibility was assessed by cytotoxicity assays. HA granules were inadequate, releasing all antibiotic during the first hours. The concentration of antibiotics released in the first days from HA-2 was higher than from HA-1 microspheres, because of the increased porosity and surface area. The release profiles (fast initial release followed by long-term sustained release) of effective doses of antibiotics make these systems good alternatives for antibiotics delivery. Osteoblasts proliferated well on both types of microspheres, being cell growth enhanced in the presence of antibiotics. Erythromycin presented the most beneficial effect. Combining the sustained antibiotic release with the osteoconduction, resorbability, and potential use as injectable bone filling material of porous HA microspheres, these systems provided a forth fold beneficial effect. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {alveolar bone, amoxicillin, amoxicillin plus clavulanic acid, Anti-Bacterial Agents, Antibacterial, antibiotic agent, antibiotic resistance, Antibiotics administration, antimicrobial activity, article, Bacteria, Cell Communication, Cell Death, cell growth, cell kinetics, Cell Line, cell proliferation, clindamycin, concentration response, controlled study, Cytotoxicity, Drug delivery, drug delivery system, Drug Delivery Systems, Drug release, Durapatite, erythromycin, Escherichia coli, human, human cell, Humans, hydroxyapatite, Kinetics, materials testing, metronidazole, Microbial Sensitivity Tests, microsphere, Microspheres, minocycline, Nanohydroxyapatite, nanoparticle, nanoparticles, nonhuman, Optical microscope analysis, osteoblast, Osteoblasts, osteolysis, periodontitis, porosity, Staphylococcus aureus, surface property, sustained drug release, Tetracycline, unclassified drug, X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249689569\&partnerID=40\&md5=5474dac8549e9cd037a96eba13ac9605},
	author = {Ferraz, M.P. and Mateus, A.Y. and Sousa, J.C. and Monteiro, F.J.}
}
@article {2386,
	title = {A novel dry active electrode for EEG recording},
	journal = {IEEE Transactions on Biomedical EngineeringIEEE Trans. Biomed. Eng.},
	volume = {54},
	year = {2007},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: ScopusArt. No.: 23CODEN: IEBEAdoi: 10.1109/TBME.2006.884649PubMed ID: 17260869Language of Original Document: EnglishCorrespondence Address: Fonseca, C.; INEB Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cfonseca@ibmc.up.ptChemicals/CAS: Biocompatible Materials; Titanium, 7440-32-6; titanium dioxide, 13463-67-7References: Portnoy, W., David, R., Akers, L., Insulated ECG electrodes (1974) Biomedical Electrode Technology, , H. A. Miller and D. C. Harrison, Eds. New York: Academic;Nishimura, S., Tomita, Y., Horiuchi, T., Clinical application of an active electrode using an operational amplifier (1992) IEEE Trans. Biomed. Eng, 39 (10), pp. 1090-1099. , Oct; 
Taheri, A., Knight, R., Smith, R., A dry electrode for EEG recording (1994) Electroencephalogr. Clin. Neurophysiol, 90, pp. 376-383; 
Searle, A., Kirkup, L., A direct comparison of wet, dry and insulating bioelectric recording electrodes (2000) Physiol. Meas, 22, pp. 71-83; 
Fonseca, C., Vaz, F., Barbosa, M.A., Electrochemical behaviour of titanium coated stainless steel by R.F. sputtering in synthetic sweat solutions for electrode applications (2004) Corrosion Sci, 46 (12), pp. 3005-3018; 
Matsuo, T., IInuma, K., Esashi, M., Barium titanate ceramics capacitive type EEG elecrode (1973) IEEE Trans. Biomed. Eng, BME-20, pp. 299-300; 
Perez, J., Vilarinho, P., Kholkin, A., High-quality Pbzr0.52Ti0. 48O3 films prepared by modified sol-gel route at low temperature (2004) Thin Solid Films, 449 (1-2), pp. 20-24; 
Rosell, J., Colominas, J., Riu, P., Pallas-Areny, R., Webster, J., Skin impedance from 1 Hz to 1 MHz (1988) IEEE Trans. Biomed. Eng, 35 (8), pp. 649-651. , Aug; 
Ferree, T., Luu, P., Russell, G., Tucker, D., Scalp electrode impedance, infection risk and EEG data quality (2001) Clin. Neurophysiol, 112 (3), pp. 536-544
},
	month = {2007///},
	pages = {162 - 165},
	abstract = {The design and testing of a "dry" active electrode for electroencephalographic recording is described. A comparative study between the EEG signals recorded in human volunteers simultaneously with the classical Ag-AgCl and "dry" active electrodes was carried out and the reported preliminary results are consistent with a better performance of these devices over the conventional Ag-AgCl electrodes. {\textcopyright} 2007 IEEE.},
	keywords = {article, Biocompatible Materials, Bioelectric phenomena, Bioelectric signal recording, bioenergy, biomedical electrodes, Biomedical engineering, Data recording, Dry electrodes, Electric Impedance, Electrochemical electrodes, electrode, Electrodes, electroencephalogram, Electroencephalographic recording, electroencephalography, Equipment Design, Equipment Failure Analysis, Feasibility Studies, Pilot Projects, signal transduction, Silver compounds, Titanium},
	isbn = {00189294 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846389173\&partnerID=40\&md5=0273ef7699807f6e20f95dd6bacb3c9b},
	author = {Fonseca, C. and Silva Cunha, J.P. and Martins, R.E. and Ferreira, V.M. and Marques De S{\'a}, J.P. and Barbosa, M.A. and Martins Da Silva, A.}
}
@article {2376,
	title = {Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix},
	journal = {BiomaterialsBiomaterials},
	volume = {28},
	year = {2007},
	note = {Cited By (since 1996): 39Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2007.04.028PubMed ID: 17532040Language of Original Document: EnglishCorrespondence Address: Evangelista, M.B.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mbe@ineb.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; alkaline phosphatase, 9001-78-9; arginylglycylaspartic acid, 99896-85-2; DNA, 9007-49-2; osteocalcin, 136461-80-8; Alginates; alginic acid, 9005-32-7; Alkaline Phosphatase, EC 3.1.3.1; arginyl-glycyl-aspartic acid, 99896-85-2; Glucuronic Acid, 576-37-4; Hexuronic Acids; Oligopeptides; Osteocalcin, 104982-03-8References: de Vos, P., de Haan, B., Wolters, G.H.J., VanSchilfgaarde, R., Factors influencing the adequacy of microencapsulation of rat pancreatic islets (1996) Transplantation, 62 (7), pp. 888-893;Brissova, M., Lacik, I., Powers, A.C., Anilkumar, A.V., Wang, T., Control and measurement of permeability for design of microcapsule cell delivery system (1998) J Biomed Mater Res, 39 (1), pp. 61-70; 
Strand, B.L., Gaserod, O., Kulseng, B., Espevik, T., Skjak-Braek, G., Alginate-polylysine-alginate microcapsules: effect of size reduction on capsule properties (2002) J Microencapsul, 19 (5), pp. 615-630; 
Chang, T.M.S., Artificial liver support based on artificial cells with emphasis on encapsulated hepatocytes (1992) Artif Organs, 16 (1), pp. 71-74; 
Uludag, H., Sefton, M.V., Microencapsulated human hepatoma (HepG2) cells-in-vitro growth and protein release (1993) J Biomed Mater Res, 27 (10), pp. 1213-1224; 
Canaple, L., Rehor, A., Hunkeler, D., Improving cell encapsulation through size control (2002) J Biomater Sci Polym Ed, 13 (7), pp. 783-796; 
Temenoff, J.S., Mikos, A.G., Injectable biodegradable materials for orthopedic tissue engineering (2000) Biomaterials, 21 (23), pp. 2405-2412; 
LeBaron, R.G., Athanasiou, K.A., Extracellular matrix cell adhesion peptides: functional applications in orthopedic materials (2000) Tissue Eng, 6 (2), pp. 85-103; 
Gumbiner, B.M., Cell adhesion: the molecular basis of tissue architecture and morphogenesis (1996) Cell, 84 (3), pp. 345-357; 
Majmudar, G., Bole, D., Goldstein, S.A., Bonadio, J., Bone cell-culture in a 3-dimensional polymer bead stabilizes the differentiated phenotype and provides evidence that osteoblastic cells synthesize type-III collagen and fibronectin (1991) J Bone Miner Res, 6 (8), pp. 869-881; 
Payne, R.G., Yaszemski, M.J., Yasko, A.W., Mikos, A.G., Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 1. Encapsulation of marrow stromal osteoblasts in surface crosslinked gelatin microparticles (2002) Biomaterials, 23 (22), pp. 4359-4371; 
Lee, K.Y., Alsberg, E., Mooney, D.J., Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering (2001) J Biomed Mater Res, 56 (2), pp. 228-233; 
Alsberg, E., Anderson, K.W., Albeiruti, A., Franceschi, R.T., Mooney, D.J., Cell-interactive alginate hydrogels for bone tissue engineering (2001) J Dental Res, 80 (11), pp. 2025-2029; 
Burdick, J.A., Anseth, K.S., Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering (2002) Biomaterials, 23 (22), pp. 4315-4323; 
Smidsrod, O., Skjak-Braek, G., Alginate as immobilization matrix for cells (1990) Trends Biotechnol, 8 (3), pp. 71-78; 
Boontheekul, T., Kong, H.J., Mooney, D.J., Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution (2005) Biomaterials, 26 (15), pp. 2455-2465; 
Kong, H.J., Alsberg, E., Kaigler, D., Lee, K.Y., Mooney, D.J., Controlling degradation of hydrogels via the size of cross-linked junctions (2004) Adv Mater, 16 (21), pp. 1917-1921; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., Alginate hydrogels as synthetic extracellular matrix materials (1999) Biomaterials, 20 (1), pp. 45-53; 
Bouhadir, K.H., Lee, K.Y., Alsberg, E., Damm, K.L., Anderson, K.W., Mooney, D.J., Degradation of partially oxidized alginate and its potential application for tissue engineering (2001) Biotechnol Prog, 17 (5), pp. 945-950; 
Kong, H.J., Smith, M.K., Mooney, D.J., Designing alginate hydrogels to maintain viability of immobilized cells (2003) Biomaterials, 24 (22), pp. 4023-4029; 
Bouhadir, K.H., Hausman, D.S., Mooney, D.J., Synthesis of cross-linked poly(aldehyde guluronate) hydrogels (1999) Polymer, 40 (12), pp. 3575-3584; 
Labarca, C., Paigen, K., Simple, rapid, and sensitive DNA assay procedure (1980) Anal Biochem, 102 (2), pp. 344-352; 
Caron, J.M., Kirschner, M.W., Autoregulation of tubulin synthesis (1986) Bioessays, 5 (5), pp. 211-216; 
Poole, C.A., Brookes, N.H., Clover, G.M., Keratocyte networks visualised in the living cornea using vital dyes (1993) J Cell Sci, 106, pp. 685-691; 
Li, R.H., Altreuter, D.H., Gentile, F.T., Transport characterization of hydrogel matrices for cell encapsulation (1996) Biotechnol Bioeng, 50 (4), pp. 365-373; 
Dulieu, C., Poncelet, D., Neufeld, R.J., Encapsulation and immobilization techniques (1999) Cell encapsulation technology and therapeutics, pp. 3-17. , K{\"u}htreiber W.M., Lanza R.P., and Chick W.L. (Eds), Birkh{\"a}user, Boston; 
Peirone, M., Ross, C.J.D., Hortelano, G., Brash, J.L., Chang, P.L., Encapsulation of various recombinant mammalian cell types in different alginate microcapsules (1998) J Biomed Mater Res, 42 (4), pp. 587-596; 
Kong, H.J., Kaigler, D., Kim, K., Mooney, D.J., Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution (2004) Biomacromolecules, 5 (5), pp. 1720-1727; 
Boninsegna, S., Dal Toso, R., Dal Monte, R., Alginate microspheres loaded with animal cells and coated by a siliceous layer (2003) J Sol-Gel Sci Technol, 26 (1-3), pp. 1151-1157; 
Rokstad, A.M., Donati, I., Borgogna, M., Oberholzer, J., Strand, B.L., Espevik, T., Cell-compatible covalently reinforced beads obtained from a chemoenzymatically engineered alginate (2006) Biomaterials, 27 (27), pp. 4726-4737; 
Lu, M.Z., Lan, H.L., Wang, F.F., Chang, S.J., Wang, Y.J., Cell encapsulation with alginate and alpha-phenoxycinnamylidene-acetylated poly(allylamine) (2000) Biotechnol Bioeng, 70 (5), pp. 479-483; 
Stein, G.S., Lian, J.B., Molecular mechanisms mediating proliferation differentiation interrelationships during progressive development of the osteoblast phenotype (1993) Endocrine Rev, 14 (4), pp. 424-442; 
Siebers, M.C., ter Brugge, P.J., Walboomers, X.F., Jansen, J.A., Integrins as linker proteins between osteoblasts and bone replacing materials. A critical review (2005) Biomaterials, 26 (2), pp. 137-146; 
Hynes, R.O., Integrins: bidirectional, allosteric signaling machines (2002) Cell, 110 (6), pp. 673-687; 
Giancotti, F.G., Ruoslahti, E., Transduction-integrin signaling (1999) Science, 285 (5430), pp. 1028-1032; 
Payne, R.G., McGonigle, J.S., Yaszemski, M.J., Yasko, A.W., Mikos, A.G., Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly(propylene fumarate) (2002) Biomaterials, 23 (22), pp. 4373-4380; 
Franceschi, R.T., Iyer, B.S., Relationship between collagen-synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells (1992) J Bone Miner Res, 7 (2), pp. 235-246; 
Franceschi, R.T., The developmental control of osteoblast-specific gene expression: role of specific transcription factors and the extracellular matrix environment (1999) Crit Rev Oral Biol Med, 10 (1), pp. 40-57; 
Gerstenfeld, L.C., Chipman, S.D., Kelly, C.M., Hodgens, K.J., Lee, D.D., Landis, W.J., Collagen expression, ultrastructural assembly, and mineralization in cultures of chicken-embryo osteoblasts (1988) J Cell Biol, 106 (3), pp. 979-989; 
Dudley, H.R., Spiro, D., Fine structure of bone cells (1961) J Biophys Biochem Cytol, 11 (3), pp. 627-649; 
Holtrop, M.E., The ultrastructure of bone (1975) Ann Clin Lab Sci, 5 (4), pp. 264-271; 
Rhodin, J.A.G., (1963) An atlas of ultrastructure, , W. B. Saunders Company, Philadelphia; 
Trojani, C., Weiss, P., Michiels, J.F., Vinatier, C., Guicheux, J., Daculsi, G., Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel (2005) Biomaterials, 26 (27), pp. 5509-5517; 
Kneser, U., Voogd, A., Ohnolz, J., Buettner, O., Stangenberg, L., Zhang, Y.H., Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute (2005) Cells Tissues Organs, 179 (4), pp. 158-169; 
Bancroft, J.D., Gamble, M., (2002) Theory and practice of histological techniques. 5th ed, , Elsevier Science, Edinburgh
},
	month = {2007///},
	pages = {3644 - 3655},
	abstract = {There is a need for new therapeutic strategies to treat bone defects caused by trauma, disease or tissue loss. Injectable systems for cell transplantation have the advantage of allowing the use of minimally invasive surgical procedures, and thus for less discomfort to patients. In the present study, it is hypothesized that Arg-Gly-Asp (RGD)-coupled in a binary (low and high molecular weight) injectable alginate composition is able to influence bone cell differentiation in a three-dimensional (3D) structure. Viability, metabolic activity, cytoskeleton organization, ultrastructure and differentiation (alkaline phosphatase (ALP), von Kossa, alizarin red stainings and osteocalcin quantification) of immobilized cells were assessed. Cells within RGD-modified alginate microspheres were able to establish more interactions with the synthetic extracellular matrix as visualized by confocal laser scanning microscope and transmission electron microscopy imaging, and presented a much higher level of differentiation (more intense ALP and mineralization stainings and higher levels of osteocalcin secretion) when compared to cells immobilized within unmodified alginate microspheres. These findings demonstrate that peptides covalently coupled to alginate were efficient in influencing cell behavior within this 3D system, and may provide adequate preparation of osteoblasts for cell transplantation. {\textcopyright} 2007 Elsevier Ltd. All rights reserved.},
	keywords = {3T3 Cells, Alginate, Alginates, alginic acid, alkaline phosphatase, animal cell, Animals, arginylglycylaspartic acid, article, Bone, bone cell, Bone Regeneration, calvaria, cell adhesion, cell differentiation, Cell encapsulation, Cell immobilization, cell metabolism, cell proliferation, cell survival, cell ultrastructure, cell viability, confocal laser microscopy, controlled study, cytology, cytoskeleton, DNA, DNA content, ECM (extracellular matrix), enzyme activity, extracellular matrix, Glucuronic Acid, Hexuronic Acids, hydrogel, immobilized cell, metabolism, Mice, Microscopy, Confocal, Microscopy, Electron, Transmission, microsphere, Microspheres, nonhuman, Oligopeptides, osteoblast, Osteoblasts, osteocalcin, Patient treatment, priority journal, rat, RGD peptide, structure analysis, three dimensional imaging, Tissue engineering, transmission electron microscopy, Transplantation (surgical)},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249870130\&partnerID=40\&md5=4476a4b1ee80320328c1bf37054037bb},
	author = {Evangelista, M.B. and Hsiong, S.X. and Fernandes, R. and Sampaio, P. and Kong, H.-J. and Barrias, C.C. and Salema, R. and Barbosa, M.A. and Mooney, D.J. and Granja, P.L.}
}
@article {2406,
	title = {Adsorption of albumin on prosthetic materials: Implication for tribological behavior},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {78},
	year = {2006},
	note = {Cited By (since 1996): 25Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30754PubMed ID: 16739106Language of Original Document: EnglishCorrespondence Address: Serro, A.P.; Centro de Qu{\'\i}mica Estrutural, Instituto Superior T{\'e}cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; email: anapaula.serro@ist.utl.ptChemicals/CAS: aluminum oxide, 1302-74-5, 1318-23-6, 1344-28-1, 14762-49-3; iodine 125, 14158-31-7, 22822-81-7; molybdenum, 7439-98-7; protein, 67254-75-5; stainless steel, 12597-68-1; Albumins; Biocompatible MaterialsTradenames: CHIRULEN, Poly Hi Solidur, GermanyManufacturers: Maret, Switzerland; MediMet, Germany; Poly Hi Solidur, GermanyReferences: Widmer, M.R., Heuberger, M., V{\"o}r{\"o}s, J., Spencer, N.D., Influence of polymer surface chemistry on frictional properties under protein-lubrication conditions: Implications for hip-implant design (2001) Tribol Lett, 10, pp. 111-116;Saikko, V., Effect of lubricant protein concentration on the wear of ultrahigh molecular weight polyethylene sliding against a CoCr counterface (2003) Trans ASME, 125, pp. 638-642; 
Sawae, Y., Murakami, T., Chen, J., Effect of synovia constituents on friction and wear of ultra-high molecular weight polyethylene sliding against prosthetic joint materials (1998) Wear, 216, pp. 213-219; 
Kumar, P., Oka, M., Ikeuchi, K., Shimizu, K., Yamamuro, T., Okumura, H., Kotoura, Y., Low wear rate of UHMWPE against zirconia ceramic (Y-PSZ) in comparison to alumina ceramic and SUS 316L alloy (1991) J Biomed Mater Res, 25, pp. 813-828; 
Kitano, T., Ateshian, G.A., Mow, V.C., Kadoya, Y., Yamano, Y., Constituents and pH changes in protein rich hyaluronan solution affect the biotribological properties of artificial cartilage (2001) J Biomech, 34, pp. 1031-1037; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., Friction and wear mechanisms in hip prosthesis: Comparison of joint materials behaviour in several lubricants (2006) Wear, 260, pp. 149-158; 
Van Enckevort, H.J., Dass, D.V., Langdon, A.G., The adsorption of bovine serum albumin at the stainless-steel/aqueous solution interface (1984) J Colloid Interface Sci, 98, pp. 138-143; 
Fukuzaki, S., Urano, H., Nagata, K., Adsorption of protein onto stainless-steel surfaces (1995) J Ferment Bioeng, 80, pp. 6-11; 
Rubio, C., Costa, D., Bellon-Fontaine, M.N., Relkin, P., Pradier, C.M., Marcus, P., Characterization of bovine serum albumin adsorption on chromium and AISI 304 stainless steel, consequences for the Pseudomonas fragi K1 adhesion (2002) Colloid Surf B Biointerface, 24, pp. 193-205; 
Sakiyama, T., Tomura, J., Imamura, K., Nakanishi, K., Adsorption characteristics of bovine serum albumin and its peptide fragments on a stainless steel surface (2004) Colloid Surf B Biointerface, 33, pp. 77-84; 
Fukuzaki, S., Urano, H., Yamaguchi, T., Effect of modification of alumina surfaces by ozone on adsorption behaviour of bovine serum albumin at alumina-water interfaces (1997) J Ferment Bioeng, 84, pp. 407-413; 
Urano, H., Fukuzaki, S., Conformation of adsorbed bovine serum albumin governing its desorption behavior at alumina-water interfaces (2000) J Ferm Bioeng, 90, pp. 105-110; 
Williams, R.L., Williams, D.F., Albumin adsorption on metal surfaces (1998) Biomaterials, 9, pp. 206-212; 
Haynes, C.A., Norde, W., Globular proteins at solid/liquid interfaces (1994) Colloid Surf B Biointerface, 2, pp. 517-566; 
Owens, D.K., Wendt, R.C., Estimation of the surface free energy of polymers (1969) J Appl Polym Sci, 13, pp. 1741-1747; 
Michalski, M.C., Hardy, J., Saramago, B., On the surface tension of PVC/EVA polymer blends: Comparison of different calculation methods (1998) J Colloid Interface Sci, 208, pp. 319-328; 
Cheng, P., Li, D., Boruvka, L., Rotenberg, Y., Neumann, A.W., Automation zof axisymmetric drop shape analysis for measurements of interfacial tensions and contact angles (1990) Colloid Surf, 43, pp. 151-197; 
Cheng, P., Neumann, A.W., Computational evaluation of Axisymmetric Drop Shape Analysis - Profile (ADSA-P) (1992) Colloid Surf, 62, pp. 297-306; 
(1990) Practical Surface Analysis, Vol. 1: Auger and X-Ray Photoelectron Spectroscopy, 1. , Briggs D, Seah MP, editors. Cichester: Wiley; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
(1993) Iodine-125, a Guide to Radioiodination Techniques, p. 64. , Little Chalfont, Buckinghamshire, UK: Amersham Life Science; 
Feng, L., Andrade, J.D., Protein adsorption on low temperature isotropic carbon. III. Isotherms, competitivity, desorption and exchange of albumin and fibrinogen (1994) Biomaterials, 15, pp. 323-333; 
Paynter, R.W., Ratner, B.D., Horbett, T.A., Thomas, H.R., XPS studies on the organization of adsorbed protein films on fluoropolymers (1984) J Colloid Interface Sci, 101, pp. 233-245; 
Paynter, R.W., Ratner, B.D., The study of interfacial proteins and biomolecules by X-ray photoelectron spectroscopy (1985) Surface and Interfacial Aspects of Biomedical Polymers, Vol. 2: Protein Adsorption, 2, pp. 189-216. , Andrade JD, editor. New York: Plenum; 
Seah, M.P., Dench, W.A., Quantitative electron spectroscopy of surfaces: A standard data base for electron inelastic mean free paths in solids (1979) Surf Interface Anal, 1, pp. 2-11; 
Tanuma, S., Powell, C.J., Penn, D.R., Calculations of electron inelastic mean free paths. II. Data for 27 elements over the 50-2000 eV range (1991) Surf Interface Anal, 17, pp. 911-926; 
Carter, D.C., He, X.M., Munson, S.H., Twigg, P.D., Gernet, K.M., Broom, M.B., Miller, T.Y., Three-dimensional structure of human serum albumin (1989) Science, 244, pp. 1195-1198; 
Pradier, C.M., Costa, D., Rubio, C., Comp{\`e}re, C., Marcus, P., Role of salts on BSA adsorption on stainless steel in aqueous solutions. I. FT-IRRAS and XPS characterization (2002) Surf Interface Anal, 34, pp. 50-54; 
Lori, J.A., Hanawa, T., Adsorption characteristics of albumin on gold and titanium metals in Hanks{\textquoteright} solution using EQCM (2004) Spectroscopy, 18, pp. 545-552; 
Sousa, S.R., Moradas-Ferreira, P., Saramago, B., Melo, L.V., Barbosa, M.A., Human serum albumin adsorption on TiO 2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Uyen, H.M.W., Schakenraad, J.M., Sjollema, J., Noordmans, J., Jongebloed, W.L., Stokroos, I., Busscher, H.J., Amount and different surface structure of albumin adsorbed to solid substrata with different wettabilities in a parallel plate flow cell (1990) J Biomed Mater Res, 24, pp. 1599-1614; 
Young, B.R., Pitt, W.G., Cooper, S.L., Protein adsorption on polymeric biomaterials. II. Adsoption kinetics (1988) J Colloid Interface Sci, 125, pp. 246-260; 
Dulm, P.V., Norde, W., The adsorption of human plasma albumin on solid surfaces, with special attention to the kinetic aspects (1983) J Colloid Interface Sci, 91, pp. 248-255; 
Malmsten, M., Formation of adsorbed protein layers (1998) J Colloid Interface Sci, 207, pp. 186-199; 
Smith, I.O., Baumann, M.J., McCabe, L.R., Electrostatic interactions as a predictor for osteoblast attachment to biomaterials (2004) J Biomed Mater Res A, 70, pp. 436-441
},
	month = {2006///},
	pages = {581 - 589},
	abstract = {The orthopedic prosthesis used to substitute damaged natural joints are lubricated by a pseudosynovial fluid that contains biological macromolecules with potential boundary lubrication properties. Proteins are some of those macromolecules whose role in the lubrication process is not yet completely understood. In a previous work, we investigated the influence of the presence of albumin, the major synovial protein, upon the tribological behavior of three of the most used pairs of artificial joint materials: ultra high molecular weight polyethylene (UHMWPE) against counterfaces of alumina, CoCrMo alloy, and 316L stainless steel. Albumin was found to cause a significant decrease in the friction coefficient when the counterfaces were metallic because transfer of UHMWPE was avoided, but this effect was much weaker in the case of alumina. The objective of the present work was to look for an explanation for these differences in tribological behavior in terms of albumin adsorption. With this goal, studies on adsorption of bovine serum albumin (BSA) on the counterface materials, from a biological model fluid (Hanks{\textquoteright} balanced salt solution), were carried out using radiolabeled albumin (                        125I-BSA), X-ray photoelectron spectroscopy, and atomic force microscopy. The conclusion from all techniques is that the driving force for albumin adsorption is higher on the metals than on alumina. These results confirm that the greater the amount of protein adsorbed on the counterface, the more efficient is the protection against the transfer of polymeric film to the counterface. {\textcopyright} 2006 Wiley Periodicals, Inc.
},
	keywords = {Adsorption, Albumin, Albumin adsorption, Albumins, Alumina, aluminum oxide, article, Atomic force microscopy, balanced salt solution, Biocompatible Materials, Body fluids, bovine serum albumin, chromium derivative, Cobalt alloy, Cobalt alloys, cobalt derivative, force, Friction, iodine 125, isotope labeling, joint fracture, Joints (anatomy), lubrication, macromolecule, metal, molecular weight, molybdenum, orthopedic prosthesis, orthopedics, Polyethylenes, polymer, Prostheses and Implants, prosthesis material, Prosthetics, protein, Proteins, Pseudosynovial fluid, Stainless steel, Thermodynamics, Tribology, ultra high molecular weight polyethylene, Ultra high molecular weight polyethylene (UHMWPE), X ray photoelectron spectroscopy},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33747426950\&partnerID=40\&md5=1728518c691365be3d5b3733dec3d60f},
	author = {Serro, A.P. and Gispert, M.P. and Martins, M.C.L. and Brogueira, P. and Cola{\c c}o, R. and Saramago, B.}
}
@article {2402,
	title = {Assessment of Bonelike{\textregistered} graft with a resorbable matrix using an animal model},
	journal = {Thin Solid FilmsThin Solid Films},
	volume = {515},
	year = {2006},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: THSFAdoi: 10.1016/j.tsf.2005.12.153Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptReferences: Hench, L.L., Wilson, J., (1993) An Introduction to Bioceramics, Singapore, , Scientific W. (Ed);Hench, L.L., (1998) Biomaterials, 19, p. 1419; 
Giannoudis, P.V., Dinopoulos, H., Tsiridis, E., (2005) Injury, 36 S, pp. S20; 
Murugan, R., Ramakrishma, S., (2005) Compos. Sci. Technol., 65, p. 2385; 
Ewers, R., Simons, B., (1992) Biomaterials-Hard tissue repair and replacement, pp. 67-80. , Elsiever, The Netherlands; 
Ilan, D.I., Ladd, A.L., (2002) Oper. Tech. Plast. Reconstr. Surg., 9, p. 151; 
Mary, E.A.R., Raymond, A.Y., (1998) Dent. Clin. North Am., 42, p. 491; 
Keating, J., McQueen, M., (2001) J. Bone Jt. Surg., 83 B, p. 3; 
Summers, B.M., Eisenstein, S.M., (2001) J. Bone Jt. Surg., 71 B, p. 677; 
Younger, E.M., Chapman, M.W., (1989) J. Orthop. Trauma, 3, p. 192; 
LeGeros, R.Z., (2002) Clin. Orthop. Res., 395, p. 81; 
Daculsi, G., (1998) Biomaterials, 19, p. 1473; 
Bohner, M., van Lenthe, G.H., Grunenfelder, S., Hirsiger, W., Evison, R., Muller, R., (2005) Biomaterials, 26, p. 6099; 
LeGeros, R.Z., (1981) Prog. Cryst. Growth Charact., 1-2, p. 1; 
LeGeros, R.Z., (1993) Dense Hydroxyapatite, An Introduction to Bioceramics, , World Scientific, Singapore; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., (1998) J. Biomed. Mater. Res., 39, p. 244; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) Biomaterials, 20, p. 2085; 
Lopes, M.A., Silva, R.F., Monteiro, F.J., Santos, J.D., (1999) Biomaterials, 21, p. 749; 
Lopes, M.A., Knowles, J.C., Santos, J.D., (2000) Biomaterials, 21, p. 1905; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Oslen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (2000) Biomaterials, 21, p. 1165; 
Mathiasen, R.A., Cruz, R.M., (2004) Otolaryngol. Head Neck Surg., 131, p. 601; 
User, H.M., Robert, B.N., (2003) Urology, 62, p. 342; 
Kheirabadi, B.S., Field-Redley, A., Pearson, R., MacPhee, M., Drohan, W., Tuthill, D., (2002) J. Surg. Res., 106, p. 99; 
Gill, I.S., Ramani, A.P., Spaliviero, M., Xu, M., Finelli, A., Kaouk, J.H., Desai, M.M., (2005) Urology, 65, p. 463; 
Weaver, F.A., Hood, D.B., Zatina, M., Messina, L., Badduke, B., (2002) Ann. Vasc. Surg., 16, p. 286; 
Ettinger, B., Genant, H.K., Cann, C.E., (1985) Ann. Intern. Med., 102, p. 319; 
Manolagas, S., Kousteni, S., Jilka, R., (2002) Recent Prog. Horm. Res., 57, p. 385; 
Trontelj, J., Vovk, T., Bogataj, M., Mrhar, A., (2005) Pharmacol. Res., 52, p. 334; 
Bryant, H., Glasebrook, A.L., Yang, N.N., Sato, M., (1999) J. Steroid Biochem. Mol. Biol., 69, p. 37; 
Bjarnason, N.H., Haarbo, J., Byrjalsen, I., Alexandersen, P., Kauffman, R.F., Christiansen, C., (2001) Atherosclerosis, 154, p. 97; 
Somjen, D., Yoles, I., (2003) J. Steroid Biochem. Mol. Biol., 86, p. 93; 
Buelke-Sam, J., Bryant, H.U., Francis, P.C., (1998) Reprod. Toxicol., 12, p. 217; 
Black, L.J., Sato, M., Rowley, E.R., Magee, D.E., Bekele, A., Williams, D.C., Cullinan, G.J., Bryant, H.U., (1994) J. Clin. Invest., 93, p. 63; 
Delmas, P.D., Bjarnason, N.H., Mitlak, B.H., Ravoux, A.C., Shah, A.S., Huster, W.J., Draper, M., Christiansen, C., (1997) N. Engl. J. Med., 337, p. 1641; 
Lobato, J.V., Sooraj Hussain, N., Botelho, C.M., Rodrigues, J.M., Luis, A.L., Mauricio, A.C., Lopes, M.A., Santos, J.D., (2005) Key Eng. Mater., 284-286, p. 877; 
Gutierres, M., Sooraj Hussain, N., Afonso, A., Almeida, L., Cabral, T., Lopes, M.A., Santos, J.D., (2005) Key Eng. Mater., 284-286, p. 1041; 
Costa, M.A., Gutierres, M., Almeida, L., Lopes, M.A., Santos, J.D., Fernandes, M.H., (2004) Key Eng. Mater., 254-256, p. 821; 
Duarte, F.M., Santos, J.D., Afonso, A., (2004) Mat. Sci. Forum, 455-456, p. 370; 
Ross, M.H., Romrell, L.J., Kaye, G.I., (1995) Histology a Text and Atlas, New York
},
	month = {2006///},
	pages = {362 - 367},
	abstract = {Synthetic bone grafts have been developed to provide an alternative to autografts and allografts. Bonelike{\textregistered} is a patented synthetic osteoconductive bone graft that mimics the mineral composition of natural bone. In the present preliminary animal studies a user-friendly version of synthetic bone graft Bonelike{\textregistered} have been developed by using a resorbable matrix, Floseal{\textregistered}, as a vehicle and raloxifene hydrochloride as a therapeutic molecule, that is known to decrease osteoclast activity and therefore enhanced bone formation. From histological and scanning electron microscopy evaluations, the use of Bonelike{\textregistered} associated with Floseal{\textregistered} and raloxifene hydrochloride showed that new bone was rapidly apposed on implanted granules and also that the presence of the matrix and therapeutic molecule does not alter the proven highly osteoconductivity properties of Bonelike{\textregistered}. Therefore, this association may be one step-forward for the clinical applications of Bonelike{\textregistered} scaffolds since it is much more easy-to-handle when compared to granular materials. {\textcopyright} 2006 Elsevier B.V. All rights reserved.},
	keywords = {animal model, Bone, Bonelike{\textregistered}, FloSeal{\textregistered}, Grafting (chemical), Histological analysis, Mathematical models, Raloxifene hydrochloride, Scanning electron microscopy},
	isbn = {00406090 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33750074198\&partnerID=40\&md5=f24aac62995c1d96ae0e344ccd428ea5},
	author = {Lobato, J.V. and Hussain, N.S. and Botelho, C.M. and Maur{\'\i}cio, A.C. and Afonso, A. and Ali, N. and Santos, J.D.}
}
@book {2414,
	title = {Calcium phosphate microspheres for localised delivery of a therapeutic enzyme},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {309-311 II},
	year = {2006},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Barrias, C.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, R. Campo Alegre 823, 4150-180 Porto, Portugal; email: ccbarrias@ineb.up.ptReferences: Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., (2004) Key Eng. Mater., 254-256, p. 877;Barrias, C.C., Ribeiro, C.C., Lamghari, M., Miranda, C.S., Barbosa, M.A., (2005) J. Biomed. Mater. Res. A, 72 A, p. 57; 
Scriver, S.R., (2001) The Metabolic Bases of Inherited Disease, , McGraw-Hill, New York; 
Sa Miranda, M.C., Aerts, J.M., Pinto, R., Fontes, A., (1990) Clin. Genet., 38, p. 218; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., (2005) Key Eng. Mater, 284-285, p. 689; 
Qi, X.Y., Grabowski, G.A., (1998) Biochem., 38, p. 11544; 
Barrias, C.C., Martins, M.A., Miranda, M.A., Barbosa, M.A., (2005) Biomaterials, 26, p. 2695
},
	month = {2006///},
	pages = {903 - 906},
	abstract = {The ability of calcium titanium phosphate (CTP) and hydroxyapatite (HAp) microspheres to reversibly adsorb the enzyme glucocerebrosidase (GCR) while preserving its biological activity, and efficiently deliver it to Gaucher disease (GD) fibroblasts was investigated. CTP microspheres adsorbed ca. 3.6-fold more GCR than HAp microspheres. The activity of adsorbed GCR was higher than the free enzyme in the case of CTP microspheres and lower when HAp was used. GCR release from both types of microspheres was characterized by the initial elution of a large percentage of enzyme followed by a delayed release that extended for at least 30 days. Released GCR was internalized by GD fibroblasts increasing their intracellular enzymatic activity. In cells treated with the same amount of GCR-loaded CTP microspheres or free-GCR a higher intracellular activity was detected in the former case, suggesting an improved efficacy.},
	keywords = {Adsorption, Biocompatibility, biological activity, Biomaterials, Calcium Compounds, Calcium titanium phosphate, Calcium titanium phosphate (CTP), Cells, Enzyme adsorption, Enzymes, hydroxyapatite, Microspheres},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645396489\&partnerID=40\&md5=de3cf8530e8c690b254fed782b964340},
	author = {Barrias, C.C. and Ribeiro, C.C. and Martins, M.C.L. and Barbosa, M.A. and Rodrigues, D. and Miranda, M.C.S.}
}
@conference {2388,
	title = {Characterization of fetal heart rate irregularity using approximate entropy and wavelet filtering},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {2006 Computers in Cardiology, CIC},
	volume = {33},
	year = {2006},
	note = {Conference code: 73228Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusArt. No.: 4512006CODEN: COCADLanguage of Original Document: EnglishCorrespondence Address: Marques De S{\'a}, J. P.; Faculdade de Engenharia, DEEC, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: jmsa@fe.up.ptReferences: Pincus, S., Approximate entropy as a measure of system complexity (1991) Proc. Natl. Acad. Sci. U.S.A, 88, pp. 2297-2301;Pincus, S., Randomness and degrees of irregularity (1996) Proc. Natl. Acad. Sci. U.S.A, 93, pp. 2083-2088; 
Pincus, S.M., Viscarello, R.R., Approximate entropy: A regularity measure for fetal heart rate (1992) Obstet. Gynecol, 2, pp. 249-255; 
Fleisher, L.A., Dipietro, J.A., Johnson, T.R., Pincus, S., Complementary and non-coincident increases in heart rate variability and irregularity during fetal development (1997) Clin. Sci, 4, pp. 345-349; 
van Leeuwen, P., Lange, S., Betterman, H., Gronemeyer, D., Hatzmann, W., Fetal heart rate variability and complexity in the course of pregnancy (1999) Early Human Develop, 54, pp. 259-269; 
Pincus, S., Assessing Serial Irregularity and its Implications for Health (2001) Ann N Y Acad Sci, 954, pp. 245-267; 
Cysarz, D., Betterman, H., van Leeuwen, P., Entropies of short binary sequences in heart period dynamics (2000) Am J Physiol Heart Circ Physiol, 6, pp. 2163-2172; 
Signorini, M.G., Magenes, G., Cerutti, S., Arduini, D., Linear and Nonlinear Parameters for the Analysis of Fetal Heart Rate Signal from Cardiotocographic Recordings (2003) IEEE Tr. BME, 50, pp. 365-373; 
Ayres-de-Campos, D., Bernardes, J., Garrido, A., Marques-de-S{\'a}, J., Pereira-Leite, L., SisPorto 2.0 - A Program for Automated Analysis of Cardiotocograms (2000) J Matern Fetal Med, 9, pp. 311-318; 
S{\'a} Couto, P.M., van Meurs, W.L., Bernardes, J.F., Marques de, S.J., Goodwin, J.A., Combined Model-Driven and Rule-Based Approach for Educational Simulation of Fetal Heart Rate (2002) Proc. 16th Biennial Int. Conf. Europ. Assoc. for Speech, Signal and Image Processing, pp. 400-402. , EURASIP, Brno, Czech Rep, pp; 
Marques de S{\'a}, J.P., Characterization of fetal heart rate using approximate entropy (2005) Proc. IEEE Comp. in Cardiology 2005, 32, pp. 671-673
},
	month = {2006///},
	pages = {933 - 936},
	address = {Valencia},
	abstract = {Approximate Entropy (ApEn) has been used by several researchers as a means to characterize the irregularity of fetal heart rate (FHR), a major contributor to fetal risk assessment. The present work starts by analyzing the influence of accelerative and decelerative (AD) components of the FHR signal in the computation of ApEn. Having shown that the AD components may completely distort the ApEn evaluation we proceed to present two methods of eliminating the influence of the AD components: wavelet filtering; AD detection using specific algorithms based on well-established physiological criteria. We obtained good results with the first method when applied to simulated signals; however it proved unreliable in the practical application of discriminating normal from pathological fetal FHR. With the second method we were able to achieve this discrimination with high statistical significance.},
	keywords = {Approximate entropy, Cardiology, Fetal heart rate, Neonatal monitoring, Occupational risks, Physiological models, risk assessment, Risk management, Safety engineering, Simulated signals, Statistical significance, Wavelet filtering},
	isbn = {02766574 (ISSN); 1424425328 (ISBN); 9781424425327 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-50149100681\&partnerID=40\&md5=51e2dd8109d66e30498d7c2f8689c820},
	author = {Magalh{\~a}es, F. and Marques De S{\'a}, J.P. and Bernardes, J. and Ayres-de-Campos, D.}
}
@book {2398,
	title = {The class imbalance problem in TLC image classification},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4142 LNCS},
	year = {2006},
	note = {Conference code: 68310Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Sousa, A.V.; Instituto Superior de Engenharia do Porto, Rua Dr. Antonio Bernardino de A. 431, 4200-072 Porto, Portugal; email: ats@isep.ipp.ptReferences: Kubat, M., Holte, R., Matwin, S., Learning when negative examples abound (1997) European Conference on Machine Learning, pp. 146-153;Lazarevi{\'c}, A., Srivastava, J., Kumar, V., Data mining for analysis of rare events: A case study in security, financial and medical applications (2004) PAKDD-2004; 
Skurichina, M., Raudys, S., Duin, R.P.W., K-nearest neighbors directed noise injection in multilayer perceptron training (2000) IEEE Transactions on Neural Networks, 11 (2); 
Chawla, N.V., Japkowicz, N., Kolcz, A., Editorial: Special issue on learning from imbalanced data sets (2004) Sigkdd Explorations, 6 (1), pp. 1-6; 
Japkowicz, N., Class imbalances: Are we focusing on the right issue? Workshop on Learning from Imbalanced Datasets II, p. 6. , (2003) ICML, Washington DC; 
Chawla, N.V., Lazarevi{\'c}, A., Hall, L.O., Bowyer, K.W., SMOTEBoost: Improving the prediction of minority class in boosting (2003) 7th European Conference on Principles and Practice of Knowledge Discovery in Databases (PKDD), pp. 107-119; 
Domingos, P., MetaCost: A general method for making classifiers cost-sensitive (1999) Conference on Knowledge Discovery in Data Proceedings of the Fifth ACM SIGKDD International Conference on Knowledge Discovery and Data Mining, pp. 155-164; 
Fan, W., Stolfo, S., Zhang, J., Chan, P., Adacost: Misclassication cost-sensitive boosting (1999) Proceedings of Sixteenth International Conference on Machine Learning, pp. 983-990; 
Tax, D.M.J., One-class classification; Concept-learning in the absence of counterexamples (2001) ASCI Dissertation Series, 65, pp. 1-190. , PhD thesis Delft University of Technology; 
Ridder, D., Tax, D.M.J., Duin, R.P.W., An experimental comparison of one-class classification methods (1998) Proc ASCI{\textquoteright}98, 4th Annual Conf of the Advanced School for Computing and Imaging, pp. 213-218. , (Lommel, Belgium, June 9-11), ASCI, Delft; 
Lazarevic, A., Ertoz, L., Ozgur, A., Srivastava, J., Kumar, V., A comparative study of anomaly detection schemes in network intrusion detection (2003) Proceedings of Third SIAM Conference on Data Mining, , San Francisco; 
Weiss, G.M., Mining with rarity: A unifying framework (2004) Sigkdd Explorations, 6 (1), pp. 7-19; 
Lewis, D.D., Gale, W.A., A sequential algorithm for training text classifiers (1994) Proceedings of International ACM Conference on Research and Development in Information Retrieval, pp. 3-12; 
Visa, S., Ralescu, A., Learning imbalanced and overlapping classes using fuzzy sets (2003) Workshop on Learning from Imbalanced Datasets II, , ICML, Washington DC; 
Sousa, A.V., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic lane and band detection in images of thin layer chromatography (2004) LNCS, 3212. , Springer; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Aguiar, R., Miranda, C.S., Feature extraction for classification of thin-layer chromatography images (2005) LNCS, 3656. , Springer; 
Japkowicz, N., Learning from imbalanced data sets: A comparison of various strategies (2000) Proceedings of Learning from Imbalanced Data, pp. 10-15; 
Kubat, M., Holte, R., Matwin, S., Machine learning for the detection of oil spills in satellite radar images (1998) Machine Learning, 30, pp. 195-215; 
Ha, T., Bunke, H., Off-Line, handwritten numeral recognition by perturbation method (1997) Pattern Analysis and Machine Intelligence, 19 (5), pp. 535-539; 
Lee, S., Noisy replication in skewed binary classification (2000) Computational Statistics \& Data Analysis; 
Heijden, F., Robert, P.W.D., Ridder, D., Tax, D.M.J., (2004) Classification, Parameter Estimation and State Estimation, , Wiley; 
Demant, C., Streicher-Abel, B., Waszkewitz, P., (1999) Industrial Image Processing, , Springer-Verlag; 
Chawla, N.V., Bowyer, K., Hall, L., Kegelmeyer, W., SMOTE: Synthetic minority over-sampling technique (2002) Jounal of Artificial Intelligence Research, 16, pp. 321-357
},
	month = {2006///},
	pages = {513 - 523},
	address = {Povoa de Varzim},
	abstract = {The paper presents the methodology developed to solve the class imbalanced problem that occurs in the classification of Thin-Layer Chromatography (TLC) images. The proposed methodology is based on resampling, and consists in the undersampling of the majority class (normal class), while the minority classes, which contain Lysosomal Storage Disorders (LSD) samples, are oversampled with the generation of synthetic samples. For image classification two approaches are presented, one based on a hierarchical classifier and another uses a multiclassifier system, where both classifiers are trained and tested using balanced data sets. The results demonstrate a better performance of the multiclassifier system using the balanced sets. {\textcopyright} Springer-Verlag Berlin Heidelberg 2006.},
	keywords = {Chromatographic analysis, Class imbalanced problem, Classification (of information), Hierarchical systems, Lysosomal Storage Disorders (LSD), Medical imaging, Multiclassifier system, Problem solving, Thin-Layer Chromatography (TLC) images},
	isbn = {03029743 (ISSN); 3540448942 (ISBN); 9783540448945 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749667604\&partnerID=40\&md5=3b4a717035de29f4bed59a04613836cd},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2404,
	title = {Differentiation of mononuclear precursors into osteoclasts on the surface of Si-substituted hydroxyapatite},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {78},
	year = {2006},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30726PubMed ID: 16739170Language of Original Document: EnglishCorrespondence Address: Brooks, R.A.; Orthopaedic Research Unit, Box 180, Addenbrooke{\textquoteright}s Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom; email: rb10003@cam.ac.ukChemicals/CAS: calcium, 7440-70-2; hydroxyapatite, 1306-06-5, 51198-94-8; phosphate, 14066-19-4, 14265-44-2; silicon, 7440-21-3; Antigens, CD14; Durapatite, 1306-06-5; Silicon, 7440-21-3References: Schilling, A., Linhart, W., Filke, S., Gebauer, M., Schinke, T., Rueger, J., Amling, M., Resorbability of bone substitute biomaterials by human osteoclasts (2004) Biomaterials, 25, pp. 3963-3972;Miller, P., Baram, D., Bilezkian, J., Greenspan, S., Lindsay, R., Riggs, B., Watts, N., Practical clinical application of biochemical markers of bone turnover: Consensus of an expert panel (1999) J Clin Densitom, 2, pp. 323-342; 
Flanagan, A., Massey, H., Wilson, C., Vellodi, A., Horton, M.A., Steward, C., Macrophage colony-stimulating factor and receptor activator NF-κ B ligand fail to rescue osteoclast-poor human malignant infantile osteopetrosis in vitro (2002) Bone, 30, pp. 85-90; 
Boyle, W., Simonet, W., Lacey, D.L., Osteoclast differentiation and activation (2003) Nature, 423, pp. 337-342; 
Susa, M., Luong-Nguyen, N.H., Cappellen, D., Zamurovic, N., Gamse, R., Human primary osteoclasts in vitro generation and applications as a pharmacological and clinical assay (2004) J Transl Med, 2, p. 6; 
Anderson, D., Maraskovsky, E., Bilingsley, W., Dougal, W., Tometsko, M., Roux, E., Teepe, M., Gilbert, L., A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function (1997) Nature, 390, pp. 175-179; 
Matsuzaki, K., Udagawa, N., Takahashi, N., Yamaguchi, K., Yasuda, H., Shima, N., Morinaga, T., Suda, T., Osteoclast differentiation factor (ODF) induces osteoclast-like cell formation in human peripheral blood mononuclear cell cultures (1998) Biochem Biophys Res Commun, 246, pp. 199-204; 
Quinn, J., Elliot, J., Gillespie, M., Martin, T., A combination of Osteoclast differentiation factor and macrophage-colony stimulating factor is sufficient for both human and mouse osteoclast formation in vitro (1998) Endocrinology, 139, pp. 4424-4427; 
Massey, H., Flanagan, A., Human osteoclasts derive from CD14-positive monocytes (1999) Br J Haematol, 106, pp. 167-170; 
Horton, M., The α vβ 3 integrin "vitronectin receptor" (1997) Int J Biochem Cell Biol, 29, pp. 721-725; 
Doi, Y., Shibutani, T., Moriwaki, Y., Kajimoto, T., Iwayama, Y., Sintered carbonate apatites as bioresorbable bone substitutes (1998) J Biomed Mater Res, 39, pp. 603-610; 
Langstaff, S., Sayer, M., Smith, T., Pugh, S., Resorbable bioceramics based on stabilized calcium phosphates. Part II: Evaluation of biological response (2001) Biomaterials, 22, pp. 135-150; 
Patel, N., Best, S.M., Gibson, I.R., Hing, K.A., Damien, E., Revell, P.A., A comparative study on the in vivo behaviour of hydroxyapatite and silicon-substituted hydroxyapatite granules (2002) J Mater Sci Mater Med, 13, pp. 1199-1206; 
Botelho, C.M., Brooks, R.A., Lopes, M.A., Santos, J.D., Best, S.M., Rushton, N., Bonfield, W., Biological and physical-chemical characterization of phase pure hydroxyapatite and silicon-substituted hydroxyapatite by different microscopy techniques (2004) Key Eng Mater, 254-256, pp. 845-849; 
Gibson, I.R., Hung, J., Best, S.M., Bonfield, W., Enhanced in vitro cell activity and surface apatite layer formation on novel silicon-substituted hydroxyapatites (1999) Bioceramics, 12, pp. 191-194. , Ohgushi H, Hastings GW, Yoshikawa T, editors. Nara, Japan: World Scientific Publishing; 
Jha, L.J., Best, S.M., Santos, J.D., Gibson, I.R., Bonfield, W., Silicon-substituted apatites and process for the preparation thereof. Worldwide Pat PCT/GB97/02325, 1999Gibson, I., Best, S.M., Bonfield, W., Chemical characterization of silicon-substituted hydroxyapatite (1999) J Biomed Mater Res, 44 B, pp. 422-428; 
Botelho, C.M., Lopes, M.A., Gibson, I.R., Best, S.M., Santos, J.D., Structural analysis of Si-substituted hydroxyapatite: Zeta potential and X-ray photoelectron spectroscopy (XPS) (2002) J Mater Sci: Mater Med, 13, pp. 1123-1127; 
Winchester, R.J., Ross, G., Methods for enumerating lymphocyte populations (1976) Manual of Clinical Immunology, pp. 64-76. , Rose NR, Friedman H, editors. Washington, DC: American Society for Microbiology; 
Burstone, M.S., Histochemical demonstration of acid phosphatases with naphthol AS-phosphate (1958) J Natl Cancer Inst, 21, pp. 423-539; 
Monchau, F., Lefevre, A., Descamps, M., Belquin-Myrdyez, A., Laffargue, P., Hildebrand, H.F., In vitro studies of human and rat osteoclast activity on hydroxyapatite, β-tricalcium phosphate, calcium carbonate (2002) Biomol Eng, 19, pp. 143-152; 
Chambers, T.J., The regulation of osteoclastic development and function (1988) Ciba Foundation Symposium, 136, pp. 92-107; 
Lakkakorpi, P., Tuukkanen, J., Hentunen, J., Jarvelin, K., Vaananen, K., Organization of osteoclast microfilaments during the attachment to bone surface in vitro (1989) J Bone Miner Res, 4, pp. 817-825; 
Nesbitt, S., Horton, M.A., Trafficking of matrix collagens through bone-resorbing osteoclasts (1997) Science, 276, pp. 266-269; 
Salo, J., Lehenkari, P., Removal of osteoclast bone resorption products by transcytosis (1997) Science, 276, pp. 270-273; 
Vaananen, K., Horton, M.A., The osteoclast clear zone is a specialized cell extracellular matrix adhesion structure (1995) J Cell Sci, 108, pp. 2729-2732; 
Kong, Y.Y., Feige, U., Sarosi, I., Bolon, B., Tafuri, A., Morony, S., Cappareli, C., Boyle, W.J., OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis (1999) Nature, 402, pp. 304-309; 
Wittrant, Y., Thepleyre, S., Couillaud, S., Dunstan, C., Heymann, D., R{\'e}dini, F., Relevance of an in vitro osteoclastogenesis system to study receptor activator of NF-κ B ligand and osteoprotegerin biological activities (2004) Exp Cell Res, 93, pp. 292-301; 
Heymann, D., gp130 cytokine family and bone cells (2000) Cytokine, 12, pp. 1455-1468; 
Heymann, D., Gouin, F., Passuti, N., Daculsi, G., Cytokines, growth factors and osteoclasts (1998) Cytokine, 10, pp. 155-168; 
Burgess, T., Qian, Y., Kaufman, S., Ring, B., Van, G., Capparelli, C., The ligand for osteoprotegerin (OPGL) directly activates mature osteoclasts (1999) J Cell Biol, 145, pp. 527-538; 
Teitelbaum, S., Bone resorption by osteoclasts (2000) Science, 289, pp. 1504-1508; 
Udagawa, N., Takahashi, N., Jimi, E., Matsuzaki, K., Tsurukai, T., Itoh, K., Nakagawa, N., Goto, M., Osteoblasts/stromal cells stimulate osteoclast activation through expression of osteoclast differentiation factor/RANKL but not macrophage colony-stimulating factor (1999) Bone, 25, pp. 517-523; 
Zhang, Y.M., Cai, Y.R., Monchau, F., Lefevre, A., Zhao, Y.M., Hildebrand, H.F., Biodegradation of synthetic bioglass with different crystallinity in vitro (2004) Seventh World Biomaterials Congress, p. 1904. , Sydney; 
Gomi, K., Lowenberg, B., Shapiro, G., Davies, J.E., Resorption of sintered synthetic hydroxyapatite by osteoclasts in vitro (1993) Biomaterials, 2, pp. 91-96; 
De Bruijin, J.D., Bovel, Y.P., Davies, J.E., Van Blitterswijik, C.A., Osteoclastic resorption of calcium phosphate is potentiated in postosteogenic culture conditions (1994) J Biomed Mater Res, 28, pp. 105-112; 
Botelho, C.M., Brooks, R.A., Kawai, T., Ogata, S., Ohtsuki, C., Best, S.M., Lopes, M.A., Bonfield, W., In vitro analysis of protein adhesion to phase pure hydroxyapatite and silicon-substituted hydroxyapatite (2005) Key Eng Mat, 284-286, pp. 461-464
},
	month = {2006///},
	pages = {709 - 720},
	abstract = {In healthy bone, resorption and synthesis are in perfect coordination. In previous studies we demonstrated that the incorporation of silicon into the hydroxyapatite (HA) lattice enhances the proliferation and differentiation of human osteoblasts. Therefore, the aim of this study was to demonstrate the effect of silicon-substituted HA (0.8 and 1.5 wt \% Si-HA) on the differentiation of mononuclear cells into osteoclasts, using two different starting cultures, peripheral blood mononuclear cells (PBMC) and monocytes expressing the CD14 antigen (CD14                        +). Through this study, it was possible to demonstrate that Si-HA allows the differentiation of mononuclear cells into mature osteoclasts, independent of the starting culture, PBMC or CD14
                        +. Most of the cells on the surface of the materials expressed osteoclastic markers: actin rings, several nuclei, positivity for tartrate-resistant acid phosphatase (TRAP), and vitronectin receptor. In the presence of osteoclasts, a higher release of calcium and phosphate into the medium from the 1.5 wt \% Si-HA substrate was detected when compared to the HA substrate; therefore, these results indicate higher osteoclastic resorptive activity on the 1.5 wt \% Si-HA surface. Si-HA can be resorbed by cellular mechanisms and have a stimulatory effect on osteoclasts, although the underlying mechanism is still poorly understood. {\textcopyright} 2006 Wiley Periodicals, Inc.
},
	keywords = {acid phosphatase tartrate resistant isoenzyme, actin, Antigens, CD14, article, Biomarkers, Calcium, CD14 antigen, cell differentiation, Cells, controlled study, Differentiation, Durapatite, human, human cell, hydroxyapatite, Microscopy, Fluorescence, monocyte, Monocytes, Monocytes CD14 positive, Osteoblasts, osteoclast, Osteoclasts, osteolysis, peripheral blood mononuclear cell, Peripheral blood mononuclear cells, phosphate, Silicon, Silicon-substituted hydroxyapatite, Sorption, Substitution reactions, Substrates, surface property, Synthesis (chemical), vitronectin receptor},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33748188416\&partnerID=40\&md5=57eb54a8d9a9fb85ada3a8fa45d323fa},
	author = {Botelho, C.M. and Brooks, R.A. and Spence, G. and McFarlane, I. and Lopes, M.A. and Best, S.M. and Santos, J.D. and Rushton, N. and Bonfield, W.}
}
@article {2405,
	title = {Error entropy in classification problems: A univariate data analysis},
	journal = {Neural ComputationNeural Comp.},
	volume = {18},
	year = {2006},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: Scopusdoi: 10.1162/neco.2006.18.9.2036PubMed ID: 16846386Language of Original Document: EnglishCorrespondence Address: Silva, L.M.; Institute de Engenharia Biom{\'e}dica, Laborat{\'o}rio Sinal e Imagem Biom{\'e}dica, 4200-465, Porto, Portugal; email: lmsilva@fe.up.ptReferences: Devroye, L., Gyorfi, L., Lugosi, G., (1996) A Probabilistic Theory of Pattern Recognition, , Berlin: Springer-Verlag;Erdogmus, D., Principe, J., Comparison of entropy and mean square error criteria in adaptive system training using higher order statistics (2000) Proceedings of the Intl. Conf., on ICA and Signal Separation, pp. 75-80. , Helsinki, Finland; 
Erdogmus, D., Principe, J.C., An error-entropy minimization algorithm for supervised training of nonlinear adaptive systems (2002) IEEE Transactions on Signal Processing, 50 (7), pp. 1780-1786; 
Kapur, J., (1993) Maximum-entropy Models in Science and Engineering (Rev. Ed), , New York: Wiley; 
Kullback, S., (1959) Statistics and Information Theory, , New York: Wiley; 
Linsker, R., Self-organization in a perceptual network (1988) IEEE Computer, 21, pp. 105-117; 
Marques De Sa, J., (2001) Pattern Recognition: Concepts, Methods and Applications, , Berlin: Springer-Verlag; 
Newman, D., Hettich, S., Blake, C., Merz, C., (1998) UCI Repository of Machine Learning Databases, , http://www.ics.uci.edu/~mlearn/MLRepository.html, Irvine: University of California, Irvine, Department of Information and Computer Sciences; 
Parzen, E., On the estimation of a probability density function and the mode (1962) Annals of Mathematical Statistics, 33, pp. 1065-1076; 
Press, W., Teukolsky, S., Vetterling, W., Flannery, B., (1992) Numerical Recipes in C: The Art of Scientific Computing, , Cambridge: Cambridge University Press; 
Principe, J.C., Xu, D., Fisher, J., Information theoretic learning (2000) Unsupervised Adaptive Filtering, Vol. 1: Blind Source Separation, 1, pp. 265-319. , S. Haykin (Ed.), New York: Wiley; 
Santos, J., Alexandre, L., Marques De S{\'a}, J., The error entropy minimization algorithm for neural network classification (2004) Int. Conf. on Recent Advances in Soft Computing, , Nottingham, U.K.: Nottingham Trent University; 
Santos, J., Marques De Sa, J., Alexandre, L., Sereno, F., Optimization of the error entropy minimization algorithm for neural network classification (2004) Intelligent Engineering Systems Through Artificial Neural Networks, 14, pp. 81-86. , C. Dagli, A. Buczak, D. Enke, M. Embrechts, \& O. Ersoy (Eds.), New York: American Society of Mechanical Engineers; 
Shannon, C.E., A mathematical theory of communication (1948) Bell System Technical Journal, 27, pp. 379-423; 
Silva, L., Marques De Sa, J., Alexandre, L., Neural network classification using Shannon{\textquoteright}s entropy (2005) European Symposium on Artificial Neural Networks, , Bruges, Belgium: d-side publications; 
Silverman, B., (1986) Density Estimation for Statistics and Data Analysis, , London: Chapman \& Hall; 
Stoller, D., Univariate two-population distribution free discrimination (1954) Journal of the American Statistical Association, 49, pp. 770-777
},
	month = {2006///},
	pages = {2036 - 2061},
	abstract = {Entropy-based cost functions are enjoying a growing attractiveness in unsupervised and supervised classification tasks. Better performances in terms both of error rate and speed of convergence have been reported. In this letter, we study the principle of error entropy minimization (EEM) from a theoretical point of view. We use Shannon{\textquoteright}s entropy and study univariate data splitting in two-class problems. In this setting, the error variable is a discrete random variable, leading to a not too complicated mathematical analysis of the error entropy. We start by showing that for uniformly distributed data, there is equivalence between the EEM split and the optimal classifier. In a more general setting, we prove the necessary conditions for this equivalence and show the existence of class configurations where the optimal classifier corresponds to maximum error entropy. The presented theoretical results provide practical guidelines that are illustrated with a set of experiments with both real and simulated data sets, where the effectiveness of EEM is compared with the usual mean square error minimization. {\textcopyright} 2006 Massachusetts Institute of Technology.},
	keywords = {article, Classification, entropy, methodology, Models, Theoretical, normal distribution, theoretical model},
	isbn = {08997667 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33748288090\&partnerID=40\&md5=47b51b3d90bc273df156889a92fef01d},
	author = {Silva, L.M. and Felgueiras, C.S. and Alexandre, L.A. and Marques de S{\'a}, J.}
}
@book {2395,
	title = {Error entropy minimization for LSTM training},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4131 LNCS - I},
	year = {2006},
	note = {Conference code: 68317Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Alexandre, L.A.; Department of Informatics, IT-Networks and Multimedia Group, University of Beira Interior, Covilh{\~a}, Portugal; email: lfbaa@di.ubi.ptReferences: Hochreiter, S., Schmidhuber, J., Long short-term memory (1997) Neural Computation, 9 (8), pp. 1735-1780;Gers, F., Schmidhuber, J., Cummins, F., Learning to forget: Continual prediction with LSTM (2000) Neural Computation, 12 (10), pp. 2451-2471; 
Gers, F., Schmidhuber, J., Recurrent nets that time and count (2000) Proc. IJCNN{\textquoteright}2000, Int. Joint Conf. on Neural Networks, , Como, Italy; 
P{\'e}rez-Ortiz, J., Gers, F., Eck, D., Schmidhuber, J., Kalman filters improve LSTM network performance in problems unsolvable by traditional recurrent nets (2003) Neural Networks, 16 (2), pp. 241-250; 
Haykin, S., (1999) Neural Networks: A Comprehensive Foundation, 2nd Edition, , Prentice Hall; 
Erdogmus, D., Principe, J., An error-entropy minimization algorithm for supervised training of nonlinear adaptive systems (2002) IEEE Trans. Signal Processing, 50 (7), pp. 1780-1786; 
Santos, J., Alexandre, L., Sereno, F., Marques De S{\'a}, J., Optimization of the error entropy minimization algorithm for neural network classification (2004) ANNIE 2004, Intelligent Engineering Systems Through Artificial Neural Networks, 14, pp. 81-86. , St.Louis, USA, ASME Press Series; 
Santos, J., Alexandre, L., Marques De S{\'a}, J., The error entropy minimization algorithm for neural network classification (2004) Proceedings of the 5th International Conference on Recent Advances in Soft Computing, pp. 92-97. , Lofti, A., ed.: Nottingham, United Kingdom; 
Silva, L., Marques De S{\'a}, J., Alexandre, L., Neural network classification using Shannon{\textquoteright}s entropy (2005) 13th European Symposium on Artificial Neural Networks - ESANN 2005, pp. 217-222. , Bruges, Belgium
},
	month = {2006///},
	pages = {244 - 253},
	address = {Athens},
	abstract = {In this paper we present a new training algorithm for the Long Short-Term Memory (LSTM) recurrent neural network. This algorithm uses entropy instead of the usual mean squared error as the cost function for the weight update. More precisely we use the Error Entropy Minimization approach, were the entropy of the error is minimized after each symbol is present to the network. Our experiments show that this approach enables the convergence of the LSTM more frequently than with the traditional learning algorithm. This in turn relaxes the burden of parameter tuning since learning is achieved for a wider range of parameter values. The use of EEM also reduces, in some cases, the number of epochs needed for convergence. {\textcopyright} Springer-Verlag Berlin Heidelberg 2006.},
	keywords = {Algorithms, Convergence of numerical methods, Cost function, entropy, Error Entropy Minimization, Errors, Long Short-Term Memory (LSTM), Neural networks, Optimization},
	isbn = {03029743 (ISSN); 3540386254 (ISBN); 9783540386254 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749837658\&partnerID=40\&md5=5f40159a3f1faf4760405dd581e79bf7},
	author = {Alexandre, L.A. and Marques De S{\'a}, J.P.}
}
@article {2392,
	title = {Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers},
	journal = {BiomaterialsBiomaterials},
	volume = {27},
	year = {2006},
	note = {Cited By (since 1996): 70Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2006.06.010PubMed ID: 16842847Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: fibrinogen, 9001-32-5; glutaraldehyde, 111-30-8, 37245-61-7; gold, 7440-57-5; thiol derivative, 13940-21-1; Alkanes; Biocompatible Materials; Fibrinogen, 9001-32-5; hydroxide ion, 14280-30-9; Hydroxides; Sulfhydryl CompoundsReferences: Savage, B., Ruggeri, Z.M., Selective recognition of adhesive sites in surface-bound fibrinogen by glycoprotein-iib-iiia on nonactivated platelets (1991) J Biol Chem, 266 (17), pp. 11227-11233;Tsai, W.B., Grunkemeier, J.M., McFarland, C.D., Horbett, T.A., Platelet adhesion to polystyrene-based surfaces preadsorbed with plasmas selectively depleted in fibrinogen, fibronectin, vitronectin, or von Willebrand{\textquoteright}s factor (2002) J Biomed Mater Res, 60 (3), pp. 348-359; 
Kottkemarchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., Effect of albumin coating on the invitro blood compatibility of dacron arterial prostheses (1989) Biomaterials, 10 (3), pp. 147-155; 
Margel, S., Vogler, E.A., Firment, L., Watt, T., Haynie, S., Sogah, D.Y., Peptide, protein, and cellular interactions with self-assembled monolayer model surfaces (1993) J Biomed Mater Res, 27 (12), pp. 1463-1476; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13 (13), pp. 3404-3413; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-terminated self-assembled monolayers (2005) Biomaterials, 26 (18), pp. 3891-3899; 
Peters, T., (1996) All about albumin: biochemistry, genetics and medical applications, , Academic Press Inc., San Diego, CA; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolavers (2003) J Biomed Mater Res A, 67 A (1), pp. 158-171; 
Frank, R.D., Dresbach, H., Thelen, H., Sieberth, H.G., Glutaraldehyde induced fluorescence technique (GIFT): a new method for the imaging of platelet adhesion on biomaterials (2000) J Biomed Mater Res, 52 (2), pp. 374-381; 
Feng, L., Andrade, J.D., Protein adsorption on low-temperature isotropic carbon. 3. Isotherms, competitivity, desorption and exchange of human albumin and fibrinogen (1994) Biomaterials, 15 (5), pp. 323-333; 
(1993) Iodine-125. A guide to radioiodination techniques, , Amersham Life Science, Little Chalfort; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., Spontaneously organized molecular assemblies. 4. Structural characterization of normal-alkyl thiol monolayers on gold by optical ellipsometry, infrared-spectroscopy, and electrochemistry (1987) J Am Chem Soc, 109 (12), pp. 3559-3568; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) J Am Chem Soc, 120 (14), pp. 3464-3473; 
Goodman, S.L., Sheep, pig, and human platelet-material interactions with model cardiovascular biomaterials (1999) J Biomed Mater Res, 45 (3), pp. 240-250; 
Slack, S.M., Horbett, T.A., Changes in fibrinogen adsorbed to segmented polyurethanes and hydroxyethylmethacrylate-ethylmethacrylate copolymers (1992) J Biomed Mater Res, 26 (12), pp. 1633-1649; 
Andrade, J.D., Hlady, V., Protein adsorption and materials biocompatibility-a tutorial review and suggested hypotheses (1986) Adv Polym Sci, 79, pp. 1-63; 
Roach, P., Farrar, D., Perry, C.C., Interpretation of protein adsorption: surface-induced conformational changes (2005) J Am Chem Soc, 127 (22), pp. 8168-8173; 
Silin, V., Weetall, H., Vanderah, D.J., SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs) (1997) J Colloid Interf Sci, 185 (1), pp. 94-103; 
Lin, Y., Wang, J., Wan, L.J., Fang, X.H., Study of fibrinogen adsorption on self-assembled monolayers on Au (1 1 1) by atomic force microscopy (2005) Ultramicroscopy, 105 (1-4), pp. 129-136; 
Norde, W., Favier, J.P., Structure of adsorbed and desorbed proteins (1992) Colloid Surf, 64 (1), pp. 87-93; 
Alfonsi-Hourdin, S., Longchamp, S., Gallet, O., Nigretto, J.M., Electrochemical processing of fibrinogen modified-graphite surfaces: effect on plasmin generation from adsorbed plasminogen (2006) Biomaterials, 27 (1), pp. 52-60; 
Tsai, W.B., Shi, Q., Grunkemeier, J.M., McFarland, C., Horbett, T.A., Platelet adhesion to radiofrequency glow-discharge-deposited fluorocarbon polymers preadsorbed with selectively depleted plasmas show the primary role of fibrinogen (2004) J Biomater Sci-Polym E, 15 (7), pp. 817-840; 
Stryer, L., (1998) Biochemistry. 4th ed., , WH Freeman, New York; 
Doolittle, R.F., Watt, K.W.K., Cottrell, B.A., Strong, D.D., Riley, M., Amino-acid sequence of the alpha-chain of human-fibrinogen (1979) Nature, 280 (5722), pp. 464-468; 
Kloczewiak, M., Timmons, S., Lukas, T.J., Hawiger, J., Platelet receptor recognition site on human-fibrinogen-synthesis and structure-function relationship of peptides corresponding to the carboxy-terminal segment of the gamma-chain (1984) Biochemistry, 23 (8), pp. 1767-1774; 
Tsai, W.B., Grunkemeier, J.M., Horbett, T.A., Variations in the ability of adsorbed fibrinogen to mediate platelet adhesion to polystyrene-based materials: a multivariate statistical analysis of antibody binding to the platelet binding sites of fibrinogen (2003) J Biomed Mater Res A, 67 A (4), pp. 1255-1268; 
Salsmann, A., Schaffner-Reckinger, E., Kabile, F., Plancon, S., Kieffer, N., A new functional role of the fibrinogen RGD motif as the molecular switch that selectively triggers integrin alpha IIb beta 3-dependent RhoA activation during cell spreading (2005) J Biol Chem, 280 (39), pp. 33610-33619; 
Lin, J.C., Chuang, W.H., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionality (2000) J Biomed Mater Res, 51 (3), pp. 413-423; 
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Kasori, Y., Shiroya, T., Blood compatible aspects of poly(2-methoxyethylacrylate) (PMEA)-relationship between protein adsorption and platelet adhesion on PMEA surface (2000) Biomaterials, 21 (14), pp. 1471-1481; 
Barbucci, R., Lamponi, S., Magnani, A., Fibrinogen conformation and platelet reactivity in relation to material-blood interaction: effect of stress hormones (2003) Biomacromolecules, 4 (6), pp. 1506-1513; 
Hylton, D.M., Shalaby, S.W., Latour, R.A., Direct correlation between adsorption-induced changes in protein structure and platelet adhesion (2005) J Biomed Mater Res A, 73 A (3), pp. 349-358; 
Brash, J.L., Tenhove, P., Protein adsorption studies on standard polymeric materials (1993) J Biomat Sci-Polym E, 4 (6), pp. 591-599
},
	month = {2006///},
	pages = {5357 - 5367},
	abstract = {The effect of surface wettability on fibrinogen adsorption, platelet adhesion and platelet activation was investigated using self-assembled monolayers (SAMs) containing different ratios of longer chain methyl- and shorter chain hydroxyl-terminated alkanethiols (C15CH3 vs. C11OH) on gold. Protein adsorption studies were performed using radiolabeled human fibrinogen (HFG). Platelet adhesion and activation studies with and without pre-adsorbed fibrinogen, albumin and plasma were assessed using scanning electron microscopy (SEM) and a glutaraldehyde-induced fluorescence technique (GIFT). Results demonstrated a linear decrease of HFG adsorption with the increase of OH groups on the monolayer (increase of the hydrophilicity). Platelet adhesion and activation also decrease with increase of hydrophilicity of surface. Concerning SAMs pre-immersed in proteins, fibrinogen adsorption was related with high platelet adhesion and activation. The passivant effect of albumin on platelet adhesion and activation was only demonstrated on SAMs contained C11OH. When all the blood proteins are present (plasma) platelet adhesion was almost absent on SAMs with 65\% and 100\% C11OH. This could be explained by the higher albumin affinity of the SAMs with 65\% C11OH and the lower total protein adsorption associated with SAMs with 100\% C11OH. {\textcopyright} 2006 Elsevier Ltd. All rights reserved.},
	keywords = {Adsorption, Albumin, alkane derivative, Alkanes, article, binding affinity, Biocompatible Materials, Blood Platelets, Cells, Cultured, Chemical reactions, controlled study, Crystallization, fibrinogen, Fluorescence, fluorescence analysis, glutaraldehyde, gold, human, human cell, Humans, hydrophilicity, Hydroxides, hydroxyl group, materials testing, methyl group, Methylation, monolayer culture, Monolayers, normal human, Organic compounds, plasma, Plasma protein, Platelet activation, Platelet adhesion, Platelet Adhesiveness, priority journal, Protein adsorption, protein binding, Proteins, Radiolabeled human fibrinogen (HFG), Scanning electron microscopy, Self assembled, SEM, Sulfhydryl Compounds, surface property, thiol derivative, thrombocyte adhesion, wettability},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33746216936\&partnerID=40\&md5=c2c54ad0448df431fbd884394915eb51},
	author = {Rodrigues, S.N. and Gon{\c c}alves, I.C. and Martins, M.C.L. and Barbosa, M.A. and Ratner, B.D.}
}
@article {2399,
	title = {Functionalization of chitosan membranes through phosphorylation: Atomic force microscopy, wettability, and cytotoxicity studies},
	journal = {Journal of Applied Polymer ScienceJ. Appl. Polym. Sci.},
	volume = {102},
	year = {2006},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JAPNAdoi: 10.1002/app.23737Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; Laborat{\'o}rio de Biomateriais, Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptReferences: Tomihata, K., Ikada, Y., (1997) Biomaterials, 18, p. 567;VandeVord, P.J., Matthew, H.W.T., DeSilva, S.P., Mayton, L., Wu, B., Wooley, P.H., (2002) J Biomed Mater Res, 59, p. 585; 
Khor, E.C., (2001) Fulfilling a Biomaterials Promise, , Elsevier: Oxford; 
Peluso, G., Petillo, O., Ranieri, M., Santin, M., Ambrosio, L., Calabro, D., Avallone, B., Balsamo, G., (1994) Biomaterials, 15, p. 1215; 
Muzzarelli, R.A.A., (1997) CeUul Mol Life Sci, 53, p. 131; 
Ishihara, M., Nakanishi, K., Ono, K., Sato, M., Kikuchi, M., Saito, Y., Yura, H., Kurita, A., (2002) Biomaterials, 23, p. 833; 
Muzzarelli, R.A.A., Zucchini, C., Ilari, P., Pugnaloni, A., Belmonte, M.M., Biagini, G., Castaldini, C., (1993) Biomaterials, 14, p. 925; 
Muzzarelli, R.A.A., Mattiolibelmonte, M., Tietz, C., Biagini, R., Ferioli, G., Brunelli, M.A., Fini, M., Biagini, G., (1994) Biomaterials, 15, p. 1075; 
Madihally, S.V., Matthew, H.W.T., (1999) Biomaterials, 20, p. 1133; 
Zhao, F., Yin, Y.J., Lu, W.W., Leong, J.C., Zhang, W.J., Zhang, J.Y., Zhang, M.F., Yao, K.D., (2002) Biomaterials, 23, p. 3227; 
Lahiji, A., Sohrabi, A., Hungerford, D.S., Frondoza, C.G., (2000) J Biomed Mater Res, 51, p. 586; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., (2005) J Biomed Mater Res Part A, 75, p. 387; 
Muzzarelli, R.A.A., Muzzarelli, C., (2002) Chitosan in Pharmacy and Chemistry, p. 233. , Muzzarelli, R. A. A.; Muzzarelli, C., Eds.; Atec Edizioni: Grottammare, Italy; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., (2002) J Controlled Release, 78, p. 187; 
Ho, M.H., Wang, D.M., Hsieh, H.J., Liu, H.C., Hsien, T.Y., Lai, J.Y., Hou, L.T., (2005) Biomaterials, 26, p. 3197; 
Amaral, I.F., Cordeiro, A., Sampaio, P., Barbosa, M.A., J Biomat Sci: Polym, , accepted; 
Chatelet, C., Damour, O., Domard, A., (2001) Biomaterials, 22, p. 261; 
Hidaka, Y., Ito, M., Mori, K., Yagasaki, H., Kafrawy, A.H., (1999) J Biomed Mater Res, 46, p. 418; 
Mucalo, M.R., Yokogawa, Y., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., (1995) J Mater Sci: Mater Med, 6, p. 658; 
Tanahashi, M., Matsuda, T., (1997) J Biomed Mater Res, 34, p. 305; 
Yokogawa, Y., Rayes, J.P., Mucalo, M.R., Toriyama, M., Kawamoto, Y., Suzuki, T., Nishizawa, K., Kamayama, T., (1997) J Mater Sci: Mater Med, 8, p. 407; 
Granja, P.L., Barbosa, M.A., Pouysegu, L., De Jeso, B., Rouais, F., Baquey, C., (2001) J Mater Sci, 36, p. 2163; 
Amaral, I.F., Granja, P.L., Barbosa, M.A., (2005) J Biomater Sci Polym Ed, 16, p. 1575; 
Amaral, I.F., Granja, P.L., Barbosa, M.A., (2004) Key Eng Mater, 254, p. 577; 
Granja, P.L., Pouysegu, L., Petraud, M., De Jeso, B., Baquey, C., Barbosa, M.A., (2001) J Appl Polym Sci, 82, p. 3341; 
Touey, G.P., Kingsport, T., (1956), U.S. Pat. 2,759,924Fricain, J.C., Granja, P.L., Barbosa, M.A., De Jeso, B., Barthe, N., Baquey, C., (2002) Biomaterials, 23, p. 971; 
Sakaguchi, T., Horikoshi, T., Nakajima, A., (1981) Agric Biol Chem, 45, p. 2191; 
Sakairi, N., Shirai, A., Miyazaki, S., Tashiro, H., Tsuji, Y., Kawahara, H., Yoshida, T., Tokura, S., (1998) Kobunshi Ronbunshu, 55, p. 212; 
Lee, Y.M., Shin, E.M., (1991) J Membr Sci, 64, p. 145; 
Varma, H.K., Yokogawa, Y., Espinosa, F.F., Kawamoto, Y., Nishizawa, K., Nagata, F., Kameyama, T., (1999) Biomaterials, 20, p. 879; 
Yokogawa, Y., Nishizawa, K., Nagata, F., Kamayama, T., (1999) Bioceramics, p. 129. , Ohgushi, H.; Hastings, G. W.; Yoshikawa, T., Eds.; World Scientific: Singapore; 
Anselme, K., (2000) Biomaterials, 21, p. 667; 
Northup, S.J., Cammack, J., (1999) Handbook of Biomaterials Evaluation: Scientific, Technical, and Clinical Testing of Implant Materials, p. 325. , Recum, A. F., Ed.; Taylor \& Francis: Philadelphia, PA; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., (2001) Polymer, 42, p. 3569; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., (1996) Carbohydr Polym, 29, p. 63; 
Grundke, K., Bogumil, T., Werner, C., Janke, A., Poschel, K., Jacobasch, H.J., (1996) Colloids Surf A, 116, p. 79; 
Kerkeb, M.L., Gonzalezcaballero, F., Chibowski, E., (1993) J Colloid Interface Sci, 159, p. 439; 
Andrade, J.D., Smith, L.M., Gregonis, D.E., (1985) Surface and Interfacial Aspects of Biomedical Polymers, p. 249. , Andrade, J. D., Ed.; Plenum: New York; 
Owens, D.K., Wendt, R.C., (1969) J Appl Polym Sci, 13, p. 1741; 
Berridge, M.V., Tan, A.S., McCoy, K.D., Wang, R., (1996) Biochemica, 4, p. 14; 
Mosmann, T., (1983) J Immunol Methods, 65, p. 55; 
Huang, R.Y.M., Moon, G.Y., Pal, R., (2001) J Membr Sci, 184, p. 1; 
Domke, J., Dannohl, S., Parak, W.J., Muller, O., Aicher, W.K., Radmacher, M., (2000) Colloids Surf B, 19, p. 367; 
MacDonald, D.E., Markovic, B., Allen, M., Somasundaran, P., Boskey, A.L., (1998) J Biomed Mater Res, 41, p. 120; 
Roberts, G.A.F., (1992) Chitin Chemistry, p. 350. , Macmillan: London; 
Andrade, J.D., (1992) Clin Mater, 11, p. 19; 
Wu, S., (1982) Polymer Interface and Adhesion, , Marcel Dekker: New York; 
Granja, P.L., Pouysegu, L., Deffieux, D., Daude, G., De Jeso, B., Labrugere, C., Baquey, C., Barbosa, M.A., (2001) J Appl Polym Sci, 82, p. 3354; 
Ratner, B.D., Caster, D.G., (1997) Surface Analysis - The Principal Techniques, p. 43. , Vickerman, J. C., Ed.; Wiley: Chichester, England; 
Barnes, A., Corkhill, P.H., Tighe, B.J., (1988) Polymer, 29, p. 2191; 
Domard, A., (1997) Advances in Chitin Science, p. 410. , Domard, A.; Roberts, G.; V{\r a}rum, K., Eds.; Jacques Andr{\'e}: Lyon, France; 
Denuziere, A., Ferrier, D., Damour, O., Domard, A., (1998) Biomaterials, 19, p. 1275; 
Wang, X.H., Ma, J.B., Feng, Q.L., Cui, F.Z., (2002) Biomaterials, 23, p. 4591; 
Wang, X.H., Ma, J.B., Wang, Y.N., He, B.L., (2002) Biomaterials, 23, p. 4167; 
Hamano, T., Chiba, D., Nakatsuka, K., Nagahata, M., Teramoto, A., Kondo, Y., Hachimori, A., Abe, K., (2002) Polym Adv Technol, 13, p. 46
},
	month = {2006///},
	pages = {276 - 284},
	abstract = {Grafting negatively charged groups such as phosphates is a well-known strategy for inducing the deposition of apatite-like layers under simulated physiological conditions. In this investigation, chitosan was phosphorylated in an attempt to enhance its osteoconduction. Chitosan membranes were phosphorylated at 30{\textdegree}C with the H                        3PO
                        4/ Et 
                        3PO
                        4/P
                        2O
                        5/butanol reaction system for periods up to 48 h. This method is an alternative to the phosphoric acid/ urea/dimethylformamide phosphorylation method, which involves the use of much higher temperatures. In this study, the effects of the phosphorylation reaction time on the surface morphology and surface free energy of phosphorylated membranes were investigated with atomic force microscopy and static-contact-angle measurements, respectively. In addition, the modified membranes were evaluated with respect to their cytotoxiciry toward bone cells through the incubation of human osteoblastic cells with extracts of the materials for two different periods: 24 and 120 h. The results revealed a reduction of the average surface roughness at the nanometer scale with increasing phosphorylation reaction time. Wettability studies showed an increase in the polar component of the surface free energy with increasing reaction time as a result of the increase in the phosphate surface concentration. Cytotoxicity studies revealed no cytotoxic effect of phosphorylated membranes on osteoblastic cells, regardless of the incubation period. {\textcopyright} 2006 Wiley Periodicals, Inc.
},
	keywords = {Atomic force microscopy, Atomic force microscopy (AFM), Biocompatibility, Biological applications, Biological applications of polymers, Biomaterials, Chitin, chitosan, Chitosan membranes, grafting, Grafting (chemical), Osteoblastic cells, Phosphorylation, Polymeric membranes, polysaccharide, Polysaccharides, surface property, toxicity, Urea},
	isbn = {00218995 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749478403\&partnerID=40\&md5=c365a50e3e767b1da90ad4ccd331440b},
	author = {Amaral, I.F. and Granja, P.L. and Melo, L.V. and Saramago, B. and Barbosa, M.A.}
}
@article {2420,
	title = {The influence of functional groups of self-assembled monolayers on fibrous capsule formation and cell recruitment},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {76},
	year = {2006},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30602Language of Original Document: EnglishCorrespondence Address: Barbosa, J.N.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: judite@ibmc.up.ptChemicals/CAS: gold, 7440-57-5; thiol derivative, 13940-21-1; Biocompatible Materials; Gold, 7440-57-5; Sulfhydryl CompoundsReferences: Babensee, J.E., Anderson, J.M., McIntire, L.V., Mikos, A.G., Host response to tissue engineered devices (1998) Adv Drug Deliv Rev, 33, pp. 111-139;Anderson, J.M., In vivo biocompatibility of implantable delivery systems and biomaterials (1994) Eur J Pharm Biopharm, 40, pp. 1-8; 
Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110; 
Tang, L., Eaton, J.W., Inflammatory responses to biomaterials (1995) Am J Clin Pathol, 103, pp. 466-471; 
Mrksich, M., Whitesides, G.M., Interactions of SAM{\textquoteright}s with proteins (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-57; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Mrkisich, M., Tailored substrates for studies of attached cell culture (1998) Cell Mol Life Sci, 54, pp. 653-662; 
Pan, S., Castner, D.G., Ratner, B.D., Multitechnique surface characterization of derivatization efficiences for hydroxyl-terminated self-assembled monolayers (1998) Langmuir, 14, pp. 3545-3550; 
Sedwick, A.D., Sin, Y.M., Edwards, J.C., Willoughby, D.A., Increased inflammatory reactivity in newly formed lining tissue (1983) J Pathol, 141, pp. 483-495; 
Castro, A.G., Esaguy, N., Macedo, P.M., {\'A}guas, A.P., Silva, M.T., Live but not heat-killed mycobacteria cause rapid chemotaxis of large numbers of eosinophils in vivo and are ingested by the attracted granulocytes (1991) Infect Immun, 59, pp. 3009-3014; 
Isaji, M., Momose, Y., Naito, J., Enhancement of inflammatory reactions in a non-immunological air pouch model in rats (1989) Br J Exp Pathol, 70, pp. 705-716; 
Martins, M.C.L., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on cibacron blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14, pp. 945-954; 
Lin, J., Chuang, W., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionality (2000) J Biomed Mater Res, 51, pp. 413-423; 
Thomsen, P., Gretzer, C., Macrophage interactions with modified materials surfaces (2001) Curr Opin Solid State Mater Sci, 5, pp. 163-176; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Tsai, M., Lin, J., Surface characterization and platelet adhesion studies of self-assembled monolayers with phosphonate ester and phosphonic acid functionalities (2001) J Biomed Mater Res, 55, pp. 554-565; 
Ostuni, E., Yan, L., Whitesides, G.M., The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver (1999) Colloids Surf B, 15, pp. 3-30; 
Yang, S., Ren, W., Park, Y., Sieving, A., Hsu, S., Nasser, S., Wooley, P.H., Diverse cellular and apoptotic responses to variant shapes of UHMWPE particles in a murine model of inflammation (2002) Biomaterials, 23, pp. 3535-3543; 
Sieving, A., Wu, B., Mayton, L., Nasser, S., Wooley, P.H., Morphological characteristics of total joint arthroplasty-derived ultrahigh molecular weight polyethylene (UHMWPE) wear debris that provoke inflammation in a murine model of inflammation (2003) J Biomed Mater Res A, 64, pp. 457-464; 
Barbosa, J.N., Barbosa, M.A., {\'A}guas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold (2004) Biomaterials, 25, pp. 2557-2563; 
Anderson, D.C., Springer, T.A., Leukocyte adhesion deficiency: An inherited defect in the Mac-1, LFA-1 and p150, 95 glycoproteins (1987) Annu Rev Med, 38, pp. 175-184; 
Murphy, R.T., Foley, J.B., Crean, P., Walsh, M.J., Reciprocal activation of leukocyte-endothelial adhesion molecules in acute coronary syndromes (2003) Int J Cardiol, 90, pp. 247-252; 
Tang, L., Wu, Y., Timmons, R.B., Fibrinogen adsorption and host tissue responses to plasma functionalized surfaces (1998) J Biomed Mater Res, 42, pp. 156-163; 
Barbosa, J.N., Madureira, P., Barbosa, M.A., {\'A}guas, A.P., The attraction of Mac-1 + phagocytes during acute inflammation by methyl-coated self-assembled monolayers (2005) Biomaterials, 26, pp. 3021-3027
},
	month = {2006///},
	pages = {737 - 743},
	abstract = {The contribution of the surface chemistry of an implant to the thickness of the fibrous capsule formed after implantation was herein investigated. For that, self-assembled monolayers (SAMs) of alkanethiols on gold with different terminal functional groups (COOH, OH, and CH                        3) were used. These surfaces were implanted in subcutaneous air pouches of BALB/c mice and the ensuing fibrous capsules were evaluated and compared with the initial inflammatory response caused by the implant. The thickness of the fibrous capsules that are under organization around the implant was measured 1 week after implantation by histology. Inflammatory exudates were collected from the air pouches 24 h after the implantation of SAMs and were analyzed by flow cytometry. A significant increase in the thickness of fibrous capsules was seen around implanted CH
                        3-terminated SAMs, and also in gold surfaces, in comparison with the air pouch wall of sham-operated mice and of COOH- and OH-covered SAMs. The CH
                        3-coated implants also recruited higher numbers of inflammatory cells; this enhancement involved a significant number of Mac-1
                        + cells. Our data indicate that implant surfaces coated with CH
                        3 induce thick fibrous capsules and this may be the result of the stronger inflammatory effect of CH
                        3 in comparison with COOH or OH chemical groups. {\textcopyright} 2005 Wiley Periodicals, Inc.
},
	keywords = {animal, animal cell, animal experiment, animal model, animal tissue, Animals, article, Bagg albino mouse, Biocompatible Materials, biomaterial, chemistry, controlled study, flow cytometry, functional group, gold, histology, implantation, Inflammation, male, Mice, Mice, Inbred BALB C, Monolayers, mouse, nonhuman, Self assembly, Self-assembled monolayers, Sulfhydryl Compounds, Surface chemistry, thiol derivative, tissue reaction, Tissue responses},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33745564665\&partnerID=40\&md5=6cbc5f976e86764eea35724a4af706f9},
	author = {Barbosa, J.N. and Madureira, P. and Barbosa, M.A. and {\'A}guas, A.P.}
}
@article {2423,
	title = {A model for educational simulation of neonatal cardiovascular pathophysiology.},
	journal = {Simulation in healthcare : journal of the Society for Simulation in HealthcareSimul Healthc},
	volume = {1 Spec no.},
	year = {2006},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusPubMed ID: 19088566Language of Original Document: EnglishCorrespondence Address: S{\'a} Couto, C.D.},
	month = {2006///},
	pages = {4 - 9},
	abstract = {Full-body patient simulators provide a technological basis for clinical education without risk to real patients. In a previous study, we described a model for educational simulation of infant cardiovascular physiology. Using essentially the same methodology, we derive a mathematical model for the cardiovascular system of a healthy 1-week-old neonate. Computer simulations of this model result in vital signs that are close to target hemodynamic variables. Simulated systemic arterial pressure waveform and left ventricular pressure-volume loop are realistic, and the system reacts appropriately to blood loss. We also adapt the model structure and change its parameters to reflect the congenital heart defects: patent ductus arteriosus, tetralogy of Fallot, complex coarctation of the aorta with patent foramen ovale, and transposition of the great arteries. Simulated vital signs are again close to target hemodynamic variables. The resulting model for neonatal cardiovascular pathophysiology is an essential step in attaining a full-body, model-driven neonatal acute care simulator.},
	keywords = {article, cardiovascular disease, Cardiovascular Diseases, cardiovascular function, Cardiovascular Physiological Phenomena, cardiovascular system, Computer simulation, congenital heart malformation, Education, Medical, Graduate, educational model, Heart Defects, Congenital, human, Humans, infant welfare, Infant, Newborn, medical education, methodology, Models, Educational, Models, Theoretical, newborn, theoretical model},
	isbn = {15592332 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58949093432\&partnerID=40\&md5=d38d3b54969381f1264feb1d4b45c8d1},
	author = {S{\'a} Couto, C.D. and van Meurs, W.L. and Goodwin, J.A. and Andriessen, P.}
}
@book {2397,
	title = {A multiclassifier approach for lung nodule classification},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4142 LNCS},
	year = {2006},
	note = {Conference code: 68310Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pereira, C.S.; Instituto de Engenharia Biom{\'e}dica, Rua Roberto Frias, 4200-465 Porto, Portugal; email: cpereira@estgl.ipv.ptReferences: Jemal, A., Murray, T., Ward, E., Samuels, A., Tiwari, R.C., Ghafoor, A., Feuer, E.J., Thun, M.J., (2005) Cancer Statistics, 2005, 55, pp. 10-30. , CA Cancer J Clin;Keserci, B., Yoshida, H., Computerized detection of pulmonary nodules in chest radiographs based on morphological features and wavelet snake model (2002) Medical Image Analysis, 6, pp. 431-447; 
Van Ginneken, B., Romeny, B.M.H., Viergever, M.A., Computer-aided diagnosis in chest radiography: A survey (2001) IEEE Transactions on Medical Imaging, 20 (12), pp. 1228-1241; 
Wei, J., Hagihara, Y., Shimizu, A., Kobatake, H., Optimal image feature set for detecting lung nodules on chest X-ray images (2002) CARS 2002; 
Van Ginneken, B., Katsuragawa, S., Ter Haar Romeny, B.M., Kunio, D., Viergever, M.A., Automatic detection of abnormalities in chest radiographs using local texture analysis (2002) IEEE Transactions on Medical Imaging, 21, pp. 139-149; 
Schilham, A.M.R., Van Ginneken, B., Loog, M., Multi-scale nodule detection in chest radiographs (2003) Lecture Notes in Computer Science, LNCS 2878, pp. 602-609; 
Shiraishi, J., Katsuragawa, S., Ikezoe, J., Matsumoto, T., Kobayashi, T., Matsu, K.-I.K., Matsui, M., Doi, K., Development of a digital image database for chest radiographs with and without a lung nodule: Receiver operating characteristic analysis of radiologists{\textquoteright} detection of pulmonary nodules (2000) American Journal of Roentgenology, 174, pp. 71-74; 
Yoshida, H., Local contralateral subtraction based on bilateral symmetry of lung for reduction of false positives in computerized detection of pulmonary nodules (2004) IEEE Transactions on Biomedical Engineering, 51, pp. 778-789; 
Suzuki, K., Shiraishi, J., Abe, H., MacMahon, H., Doi, K., False-positive reduction in computer-aided diagnostic scheme for detecting nodules in chest radiographs by means of massive training artificial neural network (2005) Academic Radiology, 12, pp. 191-201; 
Randen, T., Husoy, J.H., Filtering for texture classification: A comparative study (1999) IEEE Transactions on Pattern Analysis and Machine Intelligence, 21 (4); 
Grigorescu, S.E., Petkov, N., Kruizinga, P., Comparison of texture features based on Gabor filters (2002) IEEE Transactions on Image Processing, 11 (10), pp. 195-203; 
Kumar, A., Pang, G.K.H., Defect detection in textured materials using Gabor filters (2002) IEEE Transactions on Industry Applications, 38 (2); 
Varma, M., Zisserman, A., Unifying statistical texture classification frameworks (2004) Image and Vision Computing, 22, pp. 1175-1183; 
Leung, T., Malik, J., Representing and recognizing the visual appearance of materials using three-dimensional textons (2001) International Journal of Computer Vision, 43, pp. 29-44; 
Alexandre, L.A., Campilho, A.C., Kamel, M., On combining classifiers using sum and product rules (2001) Pattern Recognition Letters, 22, pp. 1283-1289
},
	month = {2006///},
	pages = {612 - 623},
	address = {Povoa de Varzim},
	abstract = {The aim of this paper is to examine a multiclassifier approach to the classification of the lung nodules in X-ray chest radiographs. The approach investigated here is based on an image region-based classification whose output is the information of the presence or absence of a nodule in an image region. The classification was made, essentially, in two steps: firstly, a set of rotation invariant features was extracted from the responses of a multi-scale and multi-orientation filter bank; secondly, different classifiers (multi-layer perceptrons) are designed using different features sets and trained in different data. These classifiers are further combined in order to improve the classification performance. The obtained results are promising and can be used for reducing the false-positives nodules detected in a computer-aided diagnosis system. {\textcopyright} Springer-Verlag Berlin Heidelberg 2006.},
	keywords = {Biological organs, Classification (of information), Computer aided diagnosis, False-positives nodules, Medical imaging, Multi-orientation filter bank, Radiography, X rays, X-ray chest radiographs},
	isbn = {03029743 (ISSN); 3540448942 (ISBN); 9783540448945 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749658606\&partnerID=40\&md5=f6e83e868f5b1ee08b400ee9191138cd},
	author = {Pereira, C.S. and Alexandre, L.A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@conference {2389,
	title = {New developments of the Z-EDM algorithm},
	booktitle = {Proceedings - ISDA 2006: Sixth International Conference on Intelligent Systems Design and ApplicationsProc. ISDA Sixth Int. Conf. Intelligent Syst. Design Applic.},
	series = {ISDA 2006: Sixth International Conference on Intelligent Systems Design and Applications},
	volume = {1},
	year = {2006},
	note = {Conference code: 69992Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusArt. No.: 4021587doi: 10.1109/ISDA.2006.204Language of Original Document: EnglishCorrespondence Address: Silva, L.M.; INEB - Instituto de Engenharia Biom{\'e}dica, Porto, Portugal; email: lmsilva@fe.up.ptReferences: Bishop, C., (1995) Neural Networks for Pattern Recognition, , Oxford University Press;Blake, C., Merz, C., (1998) UCI Repository of machine learning databases University of California, , http://www.ics.uci.edu/~mlearn/MLRepository.html, Irvine, Dept. of Information and Computer Sciences; 
Erdogmus, D., Principe, J.C., An Error-Entropy Minimization Algorithm for Supervised Training of Nonlinear Adaptive Systems (2002) IEEE Transactions on Signal Processing, 50 (7), pp. 1780-1786; 
Forina, M., Armanino, C., Eigenvector projection and simplified non-linear mapping of fatty acid content of Italian olive oils (1981) Ann. Chim. (Rome), 50, pp. 127-155; 
Marques de S{\'a}, J., (2001) Pattern Recognition: Concepts, Methods and Applications, , Springer Verlag; 
J. Santos, L. Alexandre, and J. Marques de S{\'a}. The Error Entropy Minimization Algorithm for Neural Network Classification. In Int. Conf. on Recent Advances in Soft Computing, Nottingham, United Kingdom, 2004Silva, L., Alexandre, L., Marques de S{\'a}, J., Neural network classification: Maximizing zero-error density (2005) LNCS, 3686, pp. 127-135. , ICAPR 2005; 
Silva, L., Marques de S{\'a}, J., Alexandre, L., Neural Network Classification using Shannon{\textquoteright}s Entropy (2005) European Symposium on Artificial Neural Networks; 
Silverman, B., (1986) Density Estimation for Statistics and Data Analysis, , Chapman \& Hall
},
	month = {2006///},
	pages = {1067 - 1072},
	address = {Jinan},
	abstract = {In this paper we address some open questions on the recently proposed Zero-Error Density Maximization algorithm for MLP training. We propose a new version of the cost function that solves a training problem encountered in previous work and prove that the use of a nonparametric density estimator preserves the optimal solution. Some experiments are reported comparing this cost function to the usual mean-square error and cross entropy cost junctions. {\textcopyright} 2006 IEEE.},
	keywords = {Algorithms, Cost functions, Mean square error, MLP training, Nonparametric density estimators, Optimization, Personnel training, Problem solving, Zero-Error Density Maximization algorithm},
	isbn = {0769525288 (ISBN); 9780769525280 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34547496988\&partnerID=40\&md5=65cadef393b0843f5b21f6534bc122ff},
	author = {Silva, L.M. and Marques de S{\'a}, J. and Alexandre, L.A.}
}
@article {2417,
	title = {The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain},
	journal = {Journal of Molecular BiologyJ. Mol. Biol.},
	volume = {356},
	year = {2006},
	note = {Cited By (since 1996): 34Export Date: 21 November 2012Source: ScopusCODEN: JMOBAdoi: 10.1016/j.jmb.2005.12.002PubMed ID: 16403517Language of Original Document: EnglishCorrespondence Address: Azevedo, J.E.; Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jazevedo@ibmc.up.ptChemicals/CAS: Amino Acids; peroxisome-targeting signal 1 receptor; Receptors, Cytoplasmic and NuclearReferences: Brown, L.A., Baker, A., Peroxisome biogenesis and the role of protein import (2003) J. Cell. Mol. Med., 7, pp. 388-400;Heiland, I., Erdmann, R., Biogenesis of peroxisomes. Topogenesis of the peroxisomal membrane and matrix proteins (2005) FEBS J., 272, pp. 2362-2372; 
Lazarow, P.B., Peroxisome biogenesis: Advances and conundrums (2003) Curr. Opin. Cell Biol., 15, pp. 489-497; 
Moyersoen, J., Choe, J., Fan, E., Hol, W.G., Michels, P.A., Biogenesis of peroxisomes and glycosomes: Trypanosomatid glycosome assembly is a promising new drug target (2004) FEMS Microbiol. Rev., 28, pp. 603-643; 
Weller, S., Gould, S.J., Valle, D., Peroxisome biogenesis disorders (2003) Annu. Rev. Genomics Hum. Genet., 4, pp. 165-211; 
Gould, S.J., Keller, G.A., Hosken, N., Wilkinson, J., Subramani, S., A conserved tripeptide sorts proteins to peroxisomes (1989) J. Cell Biol., 108, pp. 1657-1664; 
Klein, A.T., Barnett, P., Bottger, G., Konings, D., Tabak, H.F., Distel, B., Recognition of peroxisomal targeting signal type 1 by the import receptor Pex5p (2001) J. Biol. Chem., 276, pp. 15034-15041; 
Lametschwandtner, G., Brocard, C., Fransen, M., Van Veldhoven, P., Berger, J., Hartig, A., The difference in recognition of terminal tripeptides as peroxisomal targeting signal 1 between yeast and human is due to different affinities of their receptor Pex5p to the cognate signal and to residues adjacent to it (1998) J. Biol. Chem., 273, pp. 33635-33643; 
Brocard, C., Kragler, F., Simon, M.M., Schuster, T., Hartig, A., The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL (1994) Biochem. Biophys. Res. Commun., 204, pp. 1016-1022; 
Dodt, G., Braverman, N., Wong, C., Moser, A., Moser, H.W., Watkins, P., Mutations in the PTS1 receptor gene, PXR1, define complementation group 2 of the peroxisome biogenesis disorders (1995) Nature Genet., 9, pp. 115-125; 
Fransen, M., Brees, C., Baumgart, E., Vanhooren, J.C., Baes, M., Mannaerts, G.P., Van Veldhoven, P.P., Identification and characterization of the putative human peroxisomal C-terminal targeting signal import receptor (1995) J. Biol. Chem., 270, pp. 7731-7736; 
Gatto Jr., G.J., Geisbrecht, B.V., Gould, S.J., Berg, J.M., A proposed model for the PEX5-peroxisomal targeting signal-1 recognition complex (2000) Proteins: Struct. Funct. Genet., 38, pp. 241-246; 
Otera, H., Setoguchi, K., Hamasaki, M., Kumashiro, T., Shimizu, N., Fujiki, Y., Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: Conserved Pex5p WXXXF/Y motifs are critical for matrix protein import (2002) Mol. Cell. Biol., 22, pp. 1639-1655; 
Terlecky, S.R., Nuttley, W.M., McCollum, D., Sock, E., Subramani, S., The Pichia pastoris peroxisomal protein PAS8p is the receptor for the C-terminal tripeptide peroxisomal targeting signal (1995) EMBO J., 14, pp. 3627-3634; 
Gatto Jr., G.J., Geisbrecht, B.V., Gould, S.J., Berg, J.M., Peroxisomal targeting signal-1 recognition by the TPR domains of human PEX5 (2000) Nature Struct. Biol., 7, pp. 1091-1095; 
Agne, B., Meindl, N.M., Niederhoff, K., Einwachter, H., Rehling, P., Sickmann, A., Pex8p: An intraperoxisomal organizer of the peroxisomal import machinery (2003) Mol. Cell., 11, pp. 635-646; 
Albertini, M., Girzalsky, W., Veenhuis, M., Kunau, W.H., Pex12p of Saccharomyces cerevisiae is a component of a multi-protein complex essential for peroxisomal matrix protein import (2001) Eur. J. Cell Biol., 80, pp. 257-270; 
Reguenga, C., Oliveira, M.E., Gouveia, A.M., Sa-Miranda, C., Azevedo, J.E., Characterization of the mammalian peroxisomal import machinery: Pex2p. Pex5p, Pex12p, and Pex14p are subunits of the same protein assembly (2001) J. Biol. Chem., 276, pp. 29935-29942; 
Huhse, B., Rehling, P., Albertini, M., Blank, L., Meller, K., Kunau, W.-H., Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery (1998) J. Cell Biol., 140, pp. 49-60; 
Liu, H., Tan, X., Russell, K.A., Veenhuis, M., Cregg, J.M., PER3, a gene required for peroxisome biogenesis in Pichia pastoris, encodes a peroxisomal membrane protein involved in protein import (1995) J. Biol. Chem., 270, pp. 10940-10951; 
Rehling, P., Skaletz-Rorowski, A., Girzalsky, W., Voorn-Brouwer, T., Fransen, M.M., Distel, B., Pex8p, an intraperoxisomal peroxin of Saccharomyces cerevisiae required for protein transport into peroxisomes binds the PTS1 receptor pex5p (2000) J. Biol. Chem., 275, pp. 3593-3602; 
Smith, J.J., Szilard, R.K., Marelli, M., Rachubinski, R.A., The peroxin Pex17p of the yeast Yarrowia lipolytica is associated peripherally with the peroxisomal membrane and is required for the import of a subset of matrix proteins (1997) Mol. Cell. Biol., 17, pp. 2511-2520; 
Waterham, H.R., Titorenko, V.I., Haima, P., Cregg, J.M., Harder, W., Veenhuis, M., The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals (1994) J. Cell Biol., 127, pp. 737-749; 
Costa-Rodrigues, J., Carvalho, A.F., Gouveia, A.M., Fransen, M., Sa-Miranda, C., Azevedo, J.E., The N terminus of the peroxisomal cycling receptor, Pex5p, is required for redirecting the peroxisome-associated peroxin back to the cytosol (2004) J. Biol. Chem., 279, pp. 46573-46579; 
Dodt, G., Gould, S.J., Multiple, P.E.X., Genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: Evidence that PTS1 protein import is mediated by a cycling receptor (1996) J. Cell Biol., 135, pp. 1763-1774; 
Gouveia, A.M., Guimaraes, C.P., Oliveira, M.E., Reguenga, C., Sa-Miranda, C., Azevedo, J.E., Characterization of the peroxisomal cycling receptor, Pex5p, using a cell-free in vitro import system (2003) J. Biol. Chem., 278, pp. 226-232; 
Oliveira, M.E., Gouveia, A.M., Pinto, R.A., Sa-Miranda, C., Azevedo, J.E., The energetics of Pex5p-mediated peroxisomal protein import (2003) J. Biol. Chem., 278, pp. 39483-39488; 
Glover, J.R., Andrews, D.W., Rachubinski, R.A., Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer (1994) Proc. Natl Acad. Sci. USA, 91, pp. 10541-10545; 
McNew, J.A., Goodman, J.M., An oligomeric protein is imported into peroxisomes in vivo (1994) J. Cell Biol., 127, pp. 1245-1257; 
Stewart, M.Q., Esposito, R.D., Gowani, J., Goodman, J.M., Alcohol oxidase and dihydroxyacetone synthase, the abundant peroxisomal proteins of methylotrophic yeasts, assemble in different cellular compartments (2001) J. Cell Sci., 114, pp. 2863-2868; 
Walton, P.A., Hill, P.E., Subramani, S., Import of stably folded proteins into peroxisomes (1995) Mol. Biol. Cell, 6, pp. 675-683; 
Saidowsky, J., Dodt, G., Kirchberg, K., Wegner, A., Nastainczyk, W., Kunau, W.H., Schliebs, W., The di-aromatic pentapeptide repeats of the human peroxisome import receptor PEX5 are separate high affinity binding sites for the peroxisomal membrane protein PEX14 (2001) J. Biol. Chem., 276, pp. 34524-34529; 
Schliebs, W., Saidowsky, J., Agianian, B., Dodt, G., Herberg, F.W., Kunau, W.H., Recombinant human peroxisomal targeting signal receptor PEX5. Structural basis for interaction of PEX5 with PEX14 (1999) J. Biol. Chem., 274, pp. 5666-5673; 
Costa-Rodrigues, J., Carvalho, A.F., Fransen, M., Hambruch, E., Schliebs, W., Sa-Miranda, C., Azevedo, J.E., Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein (2005) J. Biol. Chem., 280, pp. 24404-24411; 
Elgersma, Y., Elgersma-Hooisma, M., Wenzel, T., McCaffery, J.M., Farquhar, M.G., Subramani, S., A mobile PTS2 receptor for peroxisomal protein import in Pichia pastoris (1998) J. Cell Biol., 140, pp. 807-820; 
Rehling, P., Marzioch, M., Niesen, F., Wittke, E., Veenhuis, M., Kunau, W.H., The import receptor for the peroxisomal targeting signal 2 (PTS2) in Saccharomyces cerevisiae is encoded by the PAS7 gene (1996) EMBO J., 15, pp. 2901-2913; 
Zhang, J.W., Lazarow, P.B., Peb1p (Pas7p) is an intraperoxisomal receptor for the NH2-terminal, type 2, peroxisomal targeting sequence of thiolase: Peb1p itself is targeted to peroxisomes by an NH2-terminal peptide (1996) J. Cell Biol., 132, pp. 325-334; 
Dodt, G., Warren, D., Becker, E., Rehling, P., Gould, S.J., Domain mapping of human PEX5 reveals functional and structural similarities to Saccharomyces cerevisiae Pex18p and Pex21p (2001) J. Biol. Chem., 276, pp. 41769-41781; 
Einwachter, H., Sowinski, S., Kunau, W.H., Schliebs, W., Yarrowia lipolytica Pex20p Saccharomyces cerevisiae Pex18p/Pex21p and mammalian Pex5pL fulfil a common function in the early steps of the peroxisomal PTS2 import pathway (2001) EMBO Rep., 2, pp. 1035-1039; 
Matsumura, T., Otera, H., Fujiki, Y., Disruption of the interaction of the longer isoform of Pex5p, Pex5pL, with Pex7p abolishes peroxisome targeting signal type 2 protein import in mammals. Study with a novel Pex5-impaired Chinese hamster ovary cell mutant (2000) J. Biol. Chem., 275, pp. 21715-21721; 
Gouveia, A.M., Guimaraes, C.P., Oliveira, M.E., Sa-Miranda, C., Azevedo, J.E., Insertion of Pex5p into the peroxisomal membrane is cargo protein-dependent (2003) J. Biol. Chem., 278, pp. 4389-4392; 
De La Torre, J.G., Hydrodynamics of segmentally flexible macromolecules (1994) Eur. Biophys. J., 23, pp. 307-322; 
Denning, D.P., Uversky, V., Patel, S.S., Fink, A.L., Rexach, M., The Saccharomyces cerevisiae nucleoporin Nup2p is a natively unfolded protein (2002) J. Biol. Chem., 277, pp. 33447-33455; 
Permyakov, S.E., Millett, I.S., Doniach, S., Permyakov, E.A., Uversky, V.N., Natively unfolded C-terminal domain of caldesmon remains substantially unstructured after the effective binding to calmodulin (2003) Proteins: Struct. Funct. Genet., 53, pp. 855-862; 
Uversky, V.N., Natively unfolded proteins: A point where biology waits for physics (2002) Protein Sci., 11, pp. 739-756; 
Lydakis-Simantiris, N., Hutchison, R.S., Betts, S.D., Barry, B.A., Yocum, C.F., Manganese stabilizing protein of photosystem II is a thermostable, natively unfolded polypeptide (1999) Biochemistry, 38, pp. 404-414; 
Sreerama, N., Woody, R.W., Estimation of protein secondary structure from circular dichroism spectra: Comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set (2000) Anal. Biochem., 287, pp. 252-260; 
Stanley, W.A., Sokolova, A., Brown, A., Clarke, D.T., Wilmanns, M., Svergun, D.I., Synergistic use of synchrotron radiation techniques for biological samples in solution: A case study on protein-ligand recognition by the peroxisomal import receptor Pex5p (2004) J. Synchrotron Radiat., 11, pp. 490-496; 
Oberg, K.A., Fink, A.L., A new attenuated total reflectance Fourier transform infrared spectroscopy method for the study of proteins in solution (1998) Anal. Biochem., 256, pp. 92-106; 
Navea, S., Tauler, R., De Juan, A., Application of the local regression method interval partial least-squares to the elucidation of protein secondary structure (2005) Anal. Biochem., 336, pp. 231-242; 
Obradovic, Z., Peng, K., Vucetic, S., Radijovac, P., Dunker, A.K., Exploiting heterogeneous sequence properties improves prediction of protein disorder (2006) Proteins, , In the press; 
Gould, S.J., Collins, C.S., Opinion: Peroxisomal-protein import: Is it really that complex? (2002) Nature Rev. Mol. Cell. Biol., 3, pp. 382-389; 
Shoemaker, B.A., Portman, J.J., Wolynes, P.G., Speeding molecular recognition by using the folding funnel: The fly-casting mechanism (2000) Proc. Natl Acad. Sci. USA, 97, pp. 8868-8873; 
Pontius, B.W., Close encounters: Why unstructured, polymeric domains can increase rates of specific macromolecular association (1993) Trends Biochem. Sci., 18, pp. 181-186; 
Tompa, P., Intrinsically unstructured proteins (2002) Trends Biochem. Sci., 27, pp. 527-533; 
Gouveia, A.M., Reguenga, C., Oliveira, M.E., Sa-Miranda, C., Azevedo, J.E., Characterization of peroxisomal Pex5p from rat liver. Pex5p in the Pex5p-Pex14p membrane complex is a transmembrane protein (2000) J. Biol. Chem., 275, pp. 32444-32451; 
Dammai, V., Subramani, S., The human peroxisomal targeting signal receptor, Pex5p, is translocated into the peroxisomal matrix and recycled to the cytosol (2001) Cell, 105, pp. 187-196; 
Imanaka, T., Small, G.M., Lazarow, P.B., Translocation of acyl-CoA oxidase into peroxisomes requires ATP hydrolysis but not a membrane potential (1987) J. Cell Biol., 105, pp. 2915-2922; 
Wendland, M., Subramani, S., Cytosol-dependent peroxisomal protein import in a permeabilized cell system (1993) J. Cell Biol., 120, pp. 675-685; 
Azevedo, J.E., Costa-Rodrigues, J., Guimaraes, C.P., Oliveira, M.E., Sa-Miranda, C., Protein translocation across the peroxisomal membrane (2004) Cell Biochem. Biophys., 41, pp. 451-468; 
Wang, D., Visser, N.V., Veenhuis, M., Van Der Klei, I.J., Physical interactions of the peroxisomal targeting signal 1 receptor pex5p, studied by fluorescence correlation spectroscopy (2003) J. Biol. Chem., 278, pp. 43340-43345; 
Oliveira, M.E., Reguenga, C., Gouveia, A.M., Guimaraes, C.P., Schliebs, W., Kunau, W.H., Mammalian Pex14p: Membrane topology and characterisation of the Pex14p-Pex14p interaction (2002) Biochim. Biophys. Acta, 1567, pp. 13-22; 
Fransen, M., Wylin, T., Brees, C., Mannaerts, G.P., Van Veldhoven, P.P., Human pex19p binds peroxisomal integral membrane proteins at regions distinct from their sorting sequences (2001) Mol. Cell. Biol., 21, pp. 4413-4424; 
Sreerama, N., Venyaminov, S.Y., Woody, R.W., Estimation of protein secondary structure from circular dichroism spectra: Inclusion of denatured proteins with native proteins in the analysis (2000) Anal. Biochem., 287, pp. 243-251; 
Vigano, C., Margolles, A., Van Veen, H.W., Konings, W.N., Ruysschaert, J.M., Secondary and tertiary structure changes of reconstituted LmrA induced by nucleotide binding or hydrolysis. a Fourier transform attenuated total reflection infrared spectroscopy and tryptophan fluorescence quenching analysis (2000) J. Biol. Chem., 275, pp. 10962-10967; 
Laurent, T.C., Killander, J., Theory of gel filtration + its experimental verification (1964) J. Chromatog., 14, pp. 217-230; 
},
	month = {2006///},
	pages = {864 - 875},
	abstract = {Targeting of most newly synthesised peroxisomal matrix proteins to the organelle requires Pex5p, the so-called PTS1 receptor. According to current models of peroxisomal biogenesis, Pex5p interacts with these proteins in the cytosol, transports them to the peroxisomal membrane and catalyses their translocation across the membrane. Presently, our knowledge on the structural details behind the interaction of Pex5p with the cargo proteins is reasonably complete. In contrast, information regarding the structure of the Pex5p N-terminal half (a region containing its peroxisomal targeting domain) is still limited. We have recently observed that the Stokes radius of this Pex5p domain is anomalously large, suggesting that this portion of the protein is either a structured elongated domain or that it adopts a low compactness conformation. Here, we address this issue using a combination of biophysical and biochemical approaches. Our results indicate that the N-terminal half of Pex5p is best described as a natively unfolded pre-molten globule-like domain. The implications of these findings on the mechanism of protein import into the peroxisome are discussed. {\textcopyright} 2005 Elsevier Ltd. All rights reserved.},
	keywords = {Amino acids, amino terminal sequence, article, biogenesis, Calorimetry, Differential Scanning, cell membrane, cell receptor, Chromatography, Gel, circular dichroism, cytosol, differential scanning calorimetry, human, Humans, infrared spectroscopy, matrix protein, Natively unfolded, Peroxisomal protein import, peroxisome, Pex5p, priority journal, Protein conformation, protein degradation, protein domain, protein folding, protein interaction, protein Pex5p, protein structure, Protein Structure, Secondary, Protein Structure, Tertiary, protein transport, pts1 receptor, PTS1-receptor, Receptors, Cytoplasmic and Nuclear, Spectroscopy, Fourier Transform Infrared, unclassified drug},
	isbn = {00222836 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-31344439778\&partnerID=40\&md5=f9fba113faa8d9af200ff33c457a7698},
	author = {Carvalho, A.F. and Costa-Rodrigues, J. and Correia, I. and Costa Pessoa, J. and Faria, T.Q. and Martins, C.L. and Fransen, M. and S{\'a}-Miranda, C. and Azevedo, J.E.}
}
@book {2413,
	title = {Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {309-311 I},
	year = {2006},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterlais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Duncan, A.C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosensors and Bioelectronics, 17, pp. 413-426;Walboomers, X.F., Contact guidance of rat fibroblasts on various implant materials (1999) Journal of Biomedical Materials Research, 47, pp. 204-212; 
Queiroz, A.C., Laser surface modifications of hydroxyapatite and glass-reinforced hydroxyapatite (2004) Biomaterials, 25, pp. 4607-4614; 
Ahmad, M., McCarthy, M.B., Gronowicz, G., An in vitro model for mineralization of human osteoblasts-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220
},
	month = {2006///},
	pages = {105 - 108},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture and microchemistry. The surface topography ultimately affects the nature and the strength of the interactions that occur at biomaterial-biological environment (cell adhesion, mobility, spreading and proliferation). Thus, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (for instance, cells). [1] There are a number of substrates that already have been studied (such as silicone, polystyrene, poly-L-lactic acid and titanium coated polystyrene) in terms of surface topography. [2] However, few studies are related to hydroxyapatite substrates. As it is well established, hydroxyapatite is a well known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense hydroxyapatite substrates was altered by using KFr excimer laser. Excimer lasers produce high-intensity, pulsed ultraviolet radiation and are especially well suited for materials processing due to their large beam cross-section area, which permits using mask projection technologies to process relatively large areas in a single step.[3].},
	keywords = {Bone, cell adhesion, Cells, Ceramic materials, Excimer laser, Excimer lasers, hydroxyapatite, Microtexture, Microtopography, morphology, Strength of materials, Surface modification, Surface topography, Surface treatment},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645413875\&partnerID=40\&md5=0234521c5cb850a0ff51ca4c64c39074},
	author = {Teixeira, S. and Queiroz, A.C. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@article {2401,
	title = {Titanium dental implants coated with Bonelike{\textregistered}: Clinical case report},
	journal = {Thin Solid FilmsThin Solid Films},
	volume = {515},
	year = {2006},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: THSFAdoi: 10.1016/j.tsf.2005.12.151Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptReferences: Murugan, R., Ramakrishna, S., (2005) Compos. Sci. Technol., 65, p. 2385;Boyle, W.J., Simonet, W.S., Lacey, D.L., (2003) Nature, 423, p. 337; 
Karabuda, C., Ozdemir, O., Tosun, T., Anil, A., Olgac, V., (2001) J. Periodontol., 72, p. 1436; 
Placko, H.E., Mishra, S., Weimer, J.J., Lucas, L.C., (2000) Int. J. Oral Maxillofac. Implants, 15, p. 355; 
Jung, Y.C., Han, C.H., Lee, I.S., Kim, H.E., (2001) Int. J. Oral Maxillofac. Implants, 16, p. 809; 
Kong, Y.M., Kim, D.H., Kim, H.E., Heo, S.J., Koak, J.Y., (2002) J. Biomed. Mater. Res., Part B Appl. Biomater., 63, p. 714; 
Schutz, R.W., Thomas, D.E., (1987) Metals Handbook. ed. 9, 13, p. 669. , Davis J.R. (Ed), American Society for Metals, Cleveland, OH; 
Tsui, Y.C., Doyle, C., Clyne, T.W., (1998) Biomaterials, 19, p. 2015; 
Lavos-Valereto, I.C., Wolynec, S., Deboni, M.C.Z., Konig Jr., B., (2001) J. Biomed. Mater. Res., Part B Appl. Biomater., 58, p. 727; 
Lacefield, W.R., (1993) An Introduction to Bioceramics, , Hench L.L., and Wilsion J. (Eds), World Scientific, Singapore; 
Golec, T.S., Krauser, J.T., (1992) Dent. Clin. North Am., 36, p. 39; 
Block, M.S., Kkent, J.N., (1992) Dent. Clin. North Am., 36, p. 27; 
Yukna, R.A., (1992) Dent. Clin. North Am., 36, p. 97; 
Baltag, I., Watanabe, K., Kusakari, H., Taoyuki, N., Miyakawa, O., Kobayashi, M., Ito, N., (2000) J. Biomed. Mater. Res., Part B Appl. Biomater., 53, p. 76; 
Wheeler, S.L., (1996) Int. J. Oral Maxillofac. Implants, 11, p. 340; 
Tsui, Y.C., Doyle, C., Clyne, T.W., (1998) Biomaterials, 19, p. 2031; 
(1981) Science and Medical Applications of Hydroxyapatite, , Conish H., Aoki H., and Sawai K. (Eds), Takyama Press Systems Centre Co., Tokyo; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Santos, J.D., Lakhan, J.J., Monteiro, F.J., (1995) Biomaterials, 16, p. 521; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., (1998) J. Biomed. Mater. Res., 39, p. 244; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) Biomaterials, 20, p. 2085; 
Lopes, M.A., Silva, R.F., Monteiro, F.J., Santos, J.D., (1999) Biomaterials, 21, p. 749; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (2000) Biomaterials, 21, p. 1165; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Oslen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., (2001) J. Mater. Sci., Mater. Med., 12, p. 629; 
Ferraz, M.P., Monteiro, F.J., Serro, A.P., Saramago, B., Gibson, I.R., Santos, J.D., (2001) Biomaterials, 22, p. 3105; 
Silva, P.L., Santos, J.D., Monteiro, F.J., Knowles, J.C., (1998) Surf. Coat. Technol., 102, p. 191; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., (1999) J. Mater. Sci., Mater. Med., 10, p. 567; 
Lobato, J.V., Sooraj Hussain, N., Sousa, R.C., Maur{\'\i}cio, A.C., Santos, J.D., (2005) Br. J. Oral Maxillofac. Surg., , (Communicated); 
Br{\"a}nemark, P.I., Svensson, B., van Steenberghe, D., (1995) Clin. Oral Implants Res., 6 (4), p. 227; 
Strnad, Z., Strand, J., Povysil, C., Urban, K.K., (2000) Int. J. Oral Maxillofac. Implants, 15, p. 483; 
Ross, M.H., Romrell, L.J., Kaye, G.I., (1995) Histology, a Text and Atlas. ed. 3, , Williams \& Wilkins, New York
},
	month = {2006///},
	pages = {279 - 284},
	abstract = {The aim of the study was to evaluate the direct bone bonding and osteointegration of the commercial pure (cp Ti) implants coated with Bonelike{\textregistered} synthetic bone graft by plasma spraying. The Bonelike{\textregistered} coated implant was placed in the mandible of a 40-year-old patient and it was removed after a healing period of 3 months with a trephine of 6 mm diameter. The structure of the coating and new bone/implant interface of retrieved samples were evaluated using scanning electron microscopy (SEM) and histological analysis using light microscopy. In vivo microstructure observations of Bonelike{\textregistered} coated retrieved implants showed excellent bone remnants on its surface without any tissue and inflammatory signs observed. The reported Bonelike{\textregistered} coated (cp Ti) implants improved primary stability, which may increase the lifetime of the implant. Bonelike{\textregistered} coated dental implants proved to be highly bioactive with extensive new bone formation and strongly bonded to Bonelike{\textregistered} coating. {\textcopyright} 2005 Elsevier B.V. All rights reserved.},
	keywords = {Bone, Bone Regeneration, Bone/implant interface, Bonelike{\textregistered} coatings, Clinical applications, Dental Implants, Dental prostheses, Hip prostheses, Medical applications, Optical microscopy, Plasma spraying},
	isbn = {00406090 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33750227720\&partnerID=40\&md5=868e14c9913670ea7c14afbf49c8959e},
	author = {Lobato, J.V. and Sooraj Hussain, N. and Botelho, C.M. and Maur{\'\i}cio, A.C. and Lobato, J.M. and Lopes, M.A. and Afonso, A. and Ali, N. and Santos, J.D.}
}
@book {2429,
	title = {Adsorption isotherm of sodium ampicillin onto dense and porous hydroxyapatite},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, Rua do Campo Alegre, 823, 4150-160 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Nikolakakis, I., Newton, J.M., Malamataris, S., (2002) European Journal of Pharmaceutical Sciences, 17 (4-5), pp. 229-238;Barroug, A., Glimcher, M.J., (2002) Journal of Orthopaedic Research, 20 (2), pp. 274-280; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterials, 22 (11), pp. 1393-1400; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2004) Key Enginnering Materials, 254-256, pp. 997-1000
},
	month = {2005///},
	pages = {387 - 390},
	abstract = {The adsorption behaviour of sodium ampicillin to dense and porous hydroxyapatite as been extensively studied through the obtention of an adsorption isotherm. This isotherm was produced at body temperature, and a wide range of antibiotic solutions were used. The adsorption behaviour was measured by UV spectroscopy. The shape of the isotherm for the two forms of the material,dense and porous, indicates that the adsorption behaviour of the antibiotic seems to be different, however this is due to the adsorption taking place within the pores of the porous material, thus showing a larger amount of antibiotic adsorbed.},
	keywords = {Adsorption, Adsorption behavior, Adsorption isotherms, ampicillin, Antibiotics, hydroxyapatite, Porous hydroxyapatite, Porous materials, Sodium ampicillin, Sodium compounds, Ultraviolet spectroscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249009270\&partnerID=40\&md5=1a365e0d97db937727266c9387789cdc},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J.}
}
@book {2425,
	title = {Assessment of the potential of Bonelike{\textregistered} graft for bone regeneration by using an animal model},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Lobato, J.V.; ICBAS-Instituto de Ci{\^e}ncias Biom{\'e}dicas de Abel Salazar, Universidade do Porto, Largo Professor Abel Salazar 2, PT-4099-003 Porto, Portugal; email: jvlobato@netcabo.ptReferences: Santos, J.D., Hastings, G.W., Knowles, J.C., Sintered Hydroxyapatite Compositions and Method for the Preparation Thereof European Patent WO 0068164Lopes, M.A., Santos, J.D., Monteiro, F.J., Push-out Testing and Histological Evaluation of Glass Reinforced Hydroxyapatite Composites Implanted in the Tibia of Rabbits (2001) J.Biomed Mater Res, 54, pp. 463-469;Lopes, M.A., Santos, J.D., Glass Reinforced Hydroxyapatite Composites; Secondary Phases Proportions and Densification Effects on Biaxial Bending Strength (1999) J.Biomed Mater Res, 48, pp. 734-740; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass Reinforced Hydroxyapatite Composites: Fracture Tougfiness and Hardness Dependence on Microstructural Characteristics (1999) Biomaterials, 2, pp. 2085-2090
},
	month = {2005///},
	pages = {877 - 880},
	abstract = {Bonelike{\textregistered} graft that mimics the inorganic composition of bone tissue has been developed and characterized over the last decade. To evaluate the osteoconductivity of Bonelike{\textregistered} two granule size ranges, one ranging from 150-250μm and the other from 250-500μm were implanted in the femurs of New Zealand White rabbits, aiming at being clinically used in different medical applications, such as dentistry and orthopaedics. In order to facilitate the medical application of the Bonelike{\textregistered} graft the use of a commercially available polymeric vehicle was also analyzed. Radiological examination, histological studies and scanning electron microscopy (SEM) analyses revealed that the surface of Bonelike{\textregistered} granules was almost completely surrounded by new bone formation after 12 weeks of implantation, which proves its highly osteoconductive behaviour.},
	keywords = {animal model, Animal models, Animals, Biomaterials, Biomedical engineering, Biomimetic materials, Bone, bone tissue, Bonelike graft, Bonelike{\textregistered} graft, Granular materials, In vivo studies, Scanning electron microscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33744512103\&partnerID=40\&md5=8f2f8e274f848eb0b105fca4880c0db5},
	author = {Lobato, J.V. and Hussein, N.S. and Botelho, C.M. and Rodrigues, J.M. and Lu{\'\i}s, A.L. and Maur{\'\i}cio, A.C. and Lopes, M.A. and Santos, J.D.}
}
@article {2458,
	title = {Bonelike{\textregistered}/PLGA hybrid materials for bone regeneration: Preparation route and physicochemical characterisation},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {16},
	year = {2005},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-005-6687-yLanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB - Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; lactic acid, 113-21-3, 50-21-5; polyglycolic acid, 26124-68-5; cyclohexane, 110-82-7; methanol, 67-56-1; polyglactin, 26780-50-7, 34346-01-5; silane, 7803-62-5; water, 7732-18-5; Durapatite, 1306-06-5; Lactic Acid, 50-21-5; Polyglycolic Acid, 26009-03-0; polylactic acid-polyglycolic acid copolymer; Polymers; SilanesTradenames: Bonelike, AldrichManufacturers: AldrichReferences: Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , JAAS;Glimcher, M.J., (1976) Handbook of Physiology, Endocrinology, , edited by R. O. Greep and E. B. Astwood (American Physiological Society, Washington D.C.); 
Shors, E.C., Holmes, R.E., (1993) An Introduction to Bioceramics, p. 181. , edited by L. L. Hench and J. Wilson (World Scientific, Hong-Kong); 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., (1998) J. Biomed. Mater. Res., 39, p. 244; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res., 48, p. 734; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) Biomaterials, 20, p. 2085; 
Lopes, M.A., Silva, R.F., Monteiro, F.J., Santos, J.D., (2000) Biomaterials, 21, p. 749; 
Lopes, M.A., Knowles, J.C., Santos, J.D., (2000) Biomaterials, 21, p. 1905; 
Jha, L.J., Santos, J.D., Knowles, J.C., (1996) J. Biomed. Mater. Res., 31, p. 481; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., (2001) J. Mater. Sci.: Mater. Med., 12, p. 629; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (2000) Biomaterials, 21, p. 1165; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mater. Res., 54, p. 463; 
Wang, M., Deb, S., Bonfield, W., (2000) Mater. Lett., 44, p. 119; 
George, I., Viel, P., Bureau, C., Suski, J., L{\'e}cayon, G., (1996) Surf. Interf. Anal., 24, p. 774; 
Liu, Q., De Wijn, J.R., De Groot, K., Van Blitterwijk, C.A., (1998) Biomaterials, 19, p. 1067; 
Ara, M., Watanabe, M., Imai, Y., (2002) Biomaterials, 23, p. 2479; 
Agrawal, C.M., Ray, R.B., (2001) J. Biomed. Mater. Res., 55, p. 141; 
Arnold, U., Lindenhayn, K., Perka, C., (2002) Biomaterials, 23, p. 2303; 
Rizzi, S.C., Heath, D.J., Coombes, A.G.A., Bock, N., Textor, M., Downes, S., (2001) J. Biomed. Mater. Res., 55, p. 475; 
Simon Jr., C.G., Khatri, C.A., Wight, S.A., Wang, F.W., (2002) J. Orthop. Res., 20, p. 473; 
Mikos, A.G., Temenoff, J.S., (2000) EJB Electr. J. Biotechn., 3 (2); 
Laurencin, C.T., Attawia, M.A., Lu, L.Q., Borden, M.D., Lu, H.H., Gorum, W.J., Lieberman, J.R., (2001) Biomaterials, 22, p. 1271; 
Takenaga, M., Yamaguchi, Y., Kitagawa, A., Ogawa, Y., Mizushima, Y., Igarashi, R., (2002) J. Contr. Rel., 79, p. 81; 
Dupraz, A.M.P., De Wijn, J.R., Van De Meer, S.A.T., De Groot, K., (1996) J. Biomed. Mater. Res., 30, p. 231; 
Sheldon, R.P., (1982) Appl. Sci. Publ., 1, p. 1; 
Zhu, P.X., Ishikawa, M., Seo, W.S., Hozumi, A., Yokogawa, Y., Koumoto, K., (2002) J. Biomed. Mater. Res., 59, p. 294; 
Hooshmand, T., Van Noort, R., Keshvad, A., (2002) Dent. Mater., 18, p. 179; 
Venhoven, B.A.M., De Gee, A.J., Werner, A., Davidson, C.L., (1994) Biomaterials, 15, p. 1152; 
Yerby, S.A., Paal, A.F., Young, P.M., Beaupr{\'e}, G.S., Ohashi, K.L., Goodman, S.B., (2000) J. Biomed. Mater. Res., 49, p. 127; 
Wang, M., Bonfield, W., (2001) Biomaterials, 22, p. 1311; 
Santos, C., Luklinska, Z.B., Clarke, R.L., Davy, K.W.M., (2001) J. Mater. Sci.: Mat. Med., 12, p. 565; 
Santos, J.D., Knowles, J.C., Hastings, G.W., in "Sintered Hydroxyapatite Compositions and Method for the Preparation Thereof," European Patent No. WO0068164 (2000)Afonso, A., Santos, J.D., Vasconcelos, M., Branco, R., Cavalheiro, J., (1996) J. Mater. Sci.: Mater. Med., 7, p. 507; 
Hoffman, R., Westheim, J.G.F., Pouwel, I., Fransen, T., Gellings, P.J., (1996) Surf. Interf. Anal., 24, p. 1; 
Rider, A.N., Arnott, D.R., (1996) Surf. Interf. Anal., 24, p. 583; 
Eufinger, S., Van Ooij, W.J., Conners, K.D., (1996) Surf. Interf. Anal., 24, p. 841
},
	month = {2005///},
	pages = {253 - 259},
	abstract = {Bonelike{\textregistered}/PLGA hybrid materials have been developed using γ-MPS as silane-coupling agent between the inorganic and organic phases for controlled drug delivery applications. Silanization showed to be more effective when cyclohexane was used as a non-polar solvent (nP method) due to a chemical interaction between Bonelike{\textregistered}, and the silane film, while by using a 95/5 (V/V) methanol/water as a polar solvent (P method), a much thinner film was achieved. Functional groups of PLGA, such as the carbonyl group (C=O), were identified using Raman and FTIR-ATR analysis and therefore these groups may be used to link therapeutic molecules. These novel hybrid materials prepared by combining silanization and post-hybridisation processes are expected to find use in medical applications of bone regeneration and as drug delivery carrier for therapeutic molecules. {\textcopyright} 2005 Springer Science + Business Media, Inc.},
	keywords = {analytic method, article, Biological materials, Bone, Bone Regeneration, carbonyl derivative, chemical bond, chemical interaction, chemical reaction, Chemical reactions, chemistry, Chemistry, Physical, cyclohexane, Drug delivery, drug delivery system, Drug therapy, Durapatite, film, Fourier transform infrared spectroscopy, Hybrid materials, hybridization, hydroxyapatite, infrared spectroscopy, Lactic Acid, methanol, methodology, Microscopy, Electron, Scanning, Molecules, physical chemistry, polyglactin, polyglycolic acid, polylactic acid polyglycolic acid copolymer, polylactic acid-polyglycolic acid copolymer, polymer, Polymers, priority journal, Raman spectrometry, Raman spectroscopy, reaction analysis, Scanning electron microscopy, silane, silane derivative, Silanes, Silanization, solvent effect, Solvents, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman, synthesis, Water, X ray, X-Rays},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-15244353590\&partnerID=40\&md5=55644ee9e5b6fa323ca5693fafe0d8d7},
	author = {Oliveira, J.M. and Miyazaki, T. and Lopes, M.A. and Ohtsuki, C. and Santos, J.D.}
}
@conference {2435,
	title = {Characterization of fetal heart rate using approximate entropy},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {Computers in Cardiology, 2005},
	volume = {32},
	year = {2005},
	note = {Conference code: 69149Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusArt. No.: 1588190CODEN: COCADdoi: 10.1109/CIC.2005.1588190Language of Original Document: EnglishCorrespondence Address: Marques de S{\'a}, J.P.; Faculdade de Engenharia, DEEC, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: jmsa@fe.up.ptReferences: Felgueiras, C., Marques de, S.J., Bernardes, J., Gama, S., Classification of Foetal Heart Rate Sequences Based on Fractal Features (1998) Medical \& Biological Engineering \& Computing, 2, pp. 197-201;Pincus, S., Approximate entropy as a measure of system complexity (1991) Proc. Natl. Acad. Sci. U.S.A, 88, pp. 2297-2301; 
Pincus, S.M., Viscarello, R.R., Approximate entropy: A regularity measure for fetal heart rate (1992) Obstet. Gynecol, 2, pp. 249-255; 
Pincus, S., Approximate Entropy (ApEn) as a Complexity Measure (1995) Chaos, 5, pp. 110-117; 
Pincus, S., Randomness and degrees of irregularity (1996) Proc. Natl. Acad. Sci. U.S.A, 93, pp. 2083-2088; 
Pincus, S., Kalman, R.E., Not all (possibly) "random" sequences are created equal (1997) Proc. Natl. Acad. Sci. U.S.A, 94, pp. 3513-3518; 
Fleisher, L.A., Dipietro, J.A., Johnson, T.R., Pincus, S., Complementary and non-coincident increases in heart rate variability and irregularity during fetal development (1997) Clin. Sci, 4, pp. 345-349; 
van Leeuwen, P., Lange, S., Betterman, H., Gronemeyer, D., Hatzmann, W., Fetal heart rate variability and complexity in the course of pregnancy (1999) Early Human Develop, 54, pp. 259-269; 
Pincus, S., Assessing Serial Irregularity and its Implications for Health (2001) Ann N Y Acad Sci, 954, pp. 245-267; 
Cysarz, D., Betterman, H., van Leeuwen, P., Entropies of short binary sequences in heart period dynamics (2000) Am J Physiol Heart Circ Physiol, 6, pp. 2163-2172; 
Ayres-de-Campos, D., Bernardes, J., Garrido, A., Marques-de-S{\'a}, J., Pereira-Leite, L., SisPorto 2.0 - A Program for Automated Analysis of Cardiotocograms (2000) J Matern Fetal Med, 9, pp. 311-318
},
	month = {2005///},
	pages = {671 - 673},
	address = {Lyon},
	abstract = {Approximate entropy (ApEn) is an interesting measure for assessing the irregularity of time series and in particular for characterizing heart rate variability. In the present paper we apply ApEn to the characterization of three patterns of fetal heart rate (FHR): calm sleep; calm vigilance; pathological flat-sinusoidal condition. We conclude that ApEn can perfectly discriminate the pathological FS pattern from the normal A and C patterns and is far better for FHR pattern discrimination than classic time-domain variability indexes used in clinical practice. {\textcopyright} 2005 IEEE.},
	keywords = {Approximate entropy (ApEn), Cardiology, Fetal heart rate, Fetal monitoring, Heart rate variability, Pathology, Sleep research, Time domain analysis},
	isbn = {02766574 (ISSN); 0780393376 (ISBN); 9780780393370 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33847143708\&partnerID=40\&md5=9c0b28217d5f8a40e2e1741bba8c4561},
	author = {Marques De S{\'a}, J.P.}
}
@article {2445,
	title = {Data parallel scheduling of operations in linear algebra on heterogeneous clusters},
	journal = {WSEAS Transactions on ComputersWSEAS Trans. Comput.},
	volume = {4},
	year = {2005},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Morais, C.; Instituto Polit{\'e}cnico de Bragan{\c c}a, Bragan{\c c}a, Portugal; email: celmorais@ipb.ptReferences: Amoura, A.K., Bampis, E., K{\"o}nig, J.-C., Scheduling algorithms for parallel gaussian elimination with communication costs (1998) IEEE Transactions on Parallel and Distributed Systems, 9 (7), pp. 679-686. , July;Bal, H., Haines, M., Approaches for integrating task and data parallelism (1998) IEEE Concurrency, 6 (3), pp. 74-84; 
Barbosa, J., Tavares, J., Padilha, A., A group block distribution strategy for a heterogeneous machine (2002) Applied Informatics, pp. 378-383. , M. H. Hamza, editor. IASTED, ACTA Press, February; 
Barbosa, J., Tavares, J., Padilha, A.J., Linear algebra algorithms in a heterogeneous cluster of personal computers (2000) Proceedings of 9th Heterogeneous Computing Workshop, pp. 147-159. , IEEE CS Press, May; 
Biazewicz, J., Dell{\textquoteright}Olmo, P., Drozdowski, M., Maczka, P., Scheduling multiprocessor tasks on parallel processors with limited availability (2003) European Journal of Operational Research, 149, pp. 377-389; 
Boudet, V., Rastello, F., Robert, Y., A proposal for a heterogeneous cluster ScaLAPACK(dense linear solvers) (1999) Proceedings of PDPTA, , CSREA Press; 
Boudet, V., Robert, Y., Scheduling heuristics for heterogeneous processors (2001) Proceedings of the International Conference on Parallel and Distributed Processing Techniques End Applications (PDPTA 2001), pp. 2109-2115. , Arabnia HR, editor, June; 
Boulet, P., Dongarra, J., Rastello, F., Robert, Y., Vivien, F., Algorithmic issues on heterogeneous computing platforms (1999) Parallel Processing Letters, 9 (2), pp. 197-213; 
Braun, T., Siegel, H., Beck, N., B{\~o}l{\~o}ni, L., Maheswaran, M., Reuther, A., Robertson, J., Yao, B., A comparison of eleven static heuristics for mapping a class of independent tasks onto heterogeneous distributed computing systems (2001) Parallel and Distributed Computing, 61, pp. 810-837; 
Choi, J., Dongarra, J., Ostrouchov, L.S., Petitet, A.P., Walker, D.W., Whaley, R.C., The design and implementation of the ScaLAPACK LU, QR, and CHOLESKY factorization routines (1996) Scientific Programming, 5, pp. 173-184; 
Dongarra, J., Walker, D., The design of linear algebra libraries for high performance computers (1993), Technical Report LAPACK Working Note 58, University of Tennessee, Knoxville, JuneGarey, M., Johnson, D., (1979) Computers and Intractability, A Guide to the Theory of NP-Completeness, , W.H. Freeman; 
Hopper, E., Turton, B.C.H., An empirical investigation of meta-heuristic and heuristic algorithms for a 2d packing problem (2001) European Journal of Operational Research, 128, pp. 34-57; 
Jansen, K., Scheduling malleable parallel tasks: An asymptotic fully polynomial time approximation scheme (2004) Algorithmica, 39, pp. 59-81; 
Kwok, Y.-K., Parallel program execution on a heterogeneous pc cluster using task duplication (2000) Proceedings of 9th Heterogeneous Computing Workshop, pp. 364-374. , IEEE CS Press, May; 
Lep{\`e}re, R., Mouni{\'e}, G., Trystram, D., An approximation algorithm for scheduling trees of malleable tasks (2002) European Journal of Operational Research, (142), pp. 242-249; 
Oh, H., Ha, S., A static scheduling heuristic for heterogeneous processors (1996) Proceedings of Europar{\textquoteright}96, 1123. , Springer Verlag, August; 
Oh-Heum, Chwa, K.-Y., Scheduling parallel tasks with individual deadlines (1999) Theoretical Computer Science, 215, pp. 209-223; 
Ramaswamy, S., Sapatnekar, S., Banerjee, P., A framework for exploiting task and data parallelism on distributed memory multicomputers (1997) IEEE Transactions on Parallel and Distributed Systems, 8 (11), pp. 1098-1116. , November; 
Sarkar, V., (1989) Partitionning and Scheduling Parallel Programs for Execution on Multiprocessors, , MIT Press, Cambridge MA; 
Shirazi, B., Wang, M., Pathak, G., Analysis and evaluation of heuristic methods for static task scheduling (1990) Journal of Parallel and Distributing Computing, 10, pp. 222-232; 
Sih, G.C., Lee, E.A., A compile-time scheduling heuristic for interconnection-constrained heterogeneous processor architectures (1993) IEEE Transactions Os Parallel and Distributed Systems, 4 (2), pp. 175-187; 
Sinnen, O., Sousa, L., Comparison of contention aware list scheduling heuristics for cluster computing (2001) Workshop on Scheduling and Resource Management for Cluster Computing (ICPP), pp. 382-387. , IEEE CS Press, September; 
Topcuoglu, H., Hariri, S., Wu, M.-Y., Task scheduling algorithms for heterogeneous processors (1999) 8th Heterogeneous Computing Workshop, , IEEE CS Press; 
Topcuoglu, H., Hariri, S., Wu, M.-Y., Performance-effective and low-complexity task scheduling for heterogeneous computing (2002) IEEE Transactions on Parallel and Distributed Systems, 13 (3), pp. 260-274. , March; 
Trystram, D., Scheduling parallel applications using malleable tasks on clusters (2001) 15th International Conference on Parallel and Distributed Processing Symposium
},
	month = {2005///},
	pages = {1440 - 1448},
	abstract = {The aim of data and task parallel scheduling for dense linear algebra kernels is to minimize the processing time of an application composed by several linear algebra kernels. The scheduling strategy presented here combines the task parallelism used when scheduling independent tasks and the data parallelism used for linear algebra kernels. This problem has been studied for scheduling independent tasks on homogeneous machines. Here it is proposed a methodology for heterogeneous clusters and it is shown that significant improvements can be achieved with this strategy.},
	keywords = {Computational complexity, Computer simulation, Heterogeneous clusters, Linear algebra, Mathematical models, Optimization, Parallel algorithms, Parallel processing systems, Parallel tasks, Static scheduling},
	isbn = {11092750 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-24644509861\&partnerID=40\&md5=d5dd6aa387a7cabccd8935372bdb5a12},
	author = {Morais, C. and Barbosa, J. and Tadeu, P.}
}
@article {2451,
	title = {Development of a system to adsorb drugs onto calcium phosphate materials},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {16},
	year = {2005},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-005-2535-3PubMed ID: 15965596Language of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: aqueiroz@ineb.up.ptChemicals/CAS: ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; hydroxyapatite, 1306-06-5, 51198-94-8; metronidazole, 39322-38-8, 443-48-1; Ampicillin, 69-53-4; Anti-Infective Agents; calcium phosphate, 10103-46-5; Calcium Phosphates; Coated Materials, Biocompatible; Delayed-Action Preparations; Drug Carriers; Metronidazole, 443-48-1References: Seymour, R.A., Heasman, P.A., Macgregor, I.D.M., (1992) Drugs, Diseases, and the Periodontium, 1. , edited by R. A. Seymour and P. A. Heasman Oxford University Press, Oxford;Carnevale, R.A., Greco, G.W., Bullard, C.M., Hanker, J.S., (1987) Biomedical Materials and Devices, p. 247. , edited by J. S. Hanker and B. L. Giammara Materials Research Society, Pittsburgh; 
Friedman, M., Steinberg, D., (1990) Pharm. Res., 7, p. 313; 
Hagiwara, S., Takamatsu, N., Tominaga, Y., Umeda, M., (1998) J. Period., 69, p. 62; 
Wright, T.L., Ellen, R.P., Lacroix, J.M., Sinnadurai, S., Mittelman, M.W., (1997) J. Period. Res., 32, p. 473; 
Golomb, G., Friedman, M., Soskolne, A., Stabholz, A., Sela, M.N., (1984) J. Dent. Res., 63, p. 1149; 
Goodson, J.M., Hogan, P.E., Dunham, S.L., (1984) J. Period., 55, p. 81; 
Somayaji, B.V., Jariwala, U., Jayachandran, P., Vidyalakshim, K., Dudhani, R.V., (1998) J. Period., 69, p. 409; 
Stoltze, K., (1992) J. Clin. Period., 19, p. 698; 
Goodson, J.M., Holborow, D., Dunn, R.L., Hogan, P., Dunham, S., (1983) J. Period., 54, p. 575; 
Yu, D., Wong, J., Matsuda, Y., Fox, J.L., Higuchi, W.I., Otsuka, M., (1992) J. Pharm. Sci., 81, p. 529; 
Nishihara, K., (1993) Clin. Mater., 12, p. 159; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mat. Res., 45, p. 376; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mat. Res., 47, p. 603; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mat. Res., 54, p. 463; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterials, 22, p. 1393; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Knowles, J.C., Santos, J.D., Hastings, G.W., World Patent, WO0068164 (1994)Myers, H.M., Tochon-Danguy, H.J., Baud, C.A., (1983) Calcif. Tissue Int., 35, p. 745; 
Ratier, A., Gibson, I.R., Best, S.M., Freche, M., Lacout, J.L., Rodrigues, F., (2001) Biomaterials, 22, p. 897; 
Ando, D.J., (1996) Ultraviolet and Visible Spectroscopy, p. 16. , John Wiley \& Sons, West Sussex
},
	month = {2005///},
	pages = {641 - 646},
	abstract = {Several studies were carried out in order to reduce the systemic use of antibiotics due to the high concentration required to provide the minimum inhibitory concentration (MIC) at infected sites. The aim of this study was to develop a system of drug adsorption onto commercial hydroxyapatite (HA, Ca                        10(PO
                        4)
                        6(OH)
                        2) and glass reinforced hydroxyapatite (GR-HA) granules. The drug will then be released for the local treatment of periodontitis. The antibiotics used in this study were metronidazole, a specific antibiotic indicated for the systemic treatment of periodontitis, and ampicillin, a wide spectrum antibiotic. UV spectroscopy was used to measure the amount of drug adsorbed onto HA and GR-HA granules. Results showed that metronidazole was unable to adsorb on the material{\textquoteright}s surface, as opposed to ampicillin which adsorbed both onto HA and GR-HA. Preliminary release kinetics studies were carried out using a flow through dissolution system. Results are discussed in terms of the influence of the different surface characteristics of the materials on the adsorption processes. {\textcopyright} 2005 Springer Science + Business Media, Inc.
},
	keywords = {Adsorption, ampicillin, Anti-Infective Agents, Antibiotics, article, Calcium Compounds, calcium phosphate, Calcium phosphates, Coated Materials, Biocompatible, concentration response, Delayed-Action Preparations, Diffusion, Dissolution, drug absorption, drug adsorption, Drug Carriers, Drug release, drug stability, glass, Glass reinforced hydroxyapatie (GR-HA), human, Humans, hydroxyapatite, Kinetics, Material{\textquoteright}s surface, Materials science, materials testing, metronidazole, minimum inhibitory concentration, Minimum inhibitory concentration (MIC), periodontitis, priority journal, Reaction kinetics, reduction, surface property, Ultraviolet spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-21344438213\&partnerID=40\&md5=5963a17bf67c04ab22d05ce113fa12b0},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J.}
}
@book {2426,
	title = {Effect of calcium phosphate addition to alginate microspheres: modulation of enzyme release kinetics and improvement of cell adhesion},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Barrias, C.C.; INEB - Institute de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, R. Campo Alegre 823, 4150-180 Porto, Portugal; email: ccbarrias@ineb.up.ptReferences: Graopwski, G.A., Leslie, N., Wenstrup, R., (1998) Blood Rev, 12, p. 115;Rowley, J.A., Madlambayan, G., Mooney, D.J., (1999) Biomaterials, 20, p. 45; 
Dalby, M.J., Di Silvio, L., Harper, E.J., Bonfield, W., (2002) Biomaterials, 23, p. 569
},
	month = {2005///},
	pages = {689 - 692},
	abstract = {In this study, the addition of calcium phosphate powders to an alginate matrix was evaluated as a strategy to modulate enzyme release-kinetics from alginate microspheres and, simultaneously, to improve cell adhesion to the polymer. Pre-adsorption of the enzyme to the ceramic powders resulted in a more adequate release pattern. The ratio of ceramic-to-polymer had a pronounced effect on osteoblast adhesion to microspheres. Cells were only able to spread on microspheres with the highest percentage of ceramic (0.4 w/w using a 1.5\% w/v alginate solution).},
	keywords = {Alginate, Bioceramics, calcium phosphate, Calcium titanium phosphate, cell adhesion, Enzyme kinetics, Enzyme release, hydroxyapatite, Microspheres, Osteoblasts},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-20744433251\&partnerID=40\&md5=30ce8fc67d1e3e37074cc056f650b798},
	author = {Barrias, C.C. and Ribeiro, C.C. and Rodrigues, D. and Miranda, M.C.S. and Barbosa, M.A.}
}
@book {2439,
	title = {Feature extraction for classification of thin-layer chromatography images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {3656 LNCS},
	year = {2005},
	note = {Conference code: 67124Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1007/11559573_118Language of Original Document: EnglishCorrespondence Address: Sousa, A.V.; Instituto de Engenharia Biom{\'e}dica, Rua Roberto Frias, 4200-465 Porto, Portugal; email: ats@isep.ipp.ptReferences: Bajla, I., Holl{\"a}nder, I., Burg, K., Improvement of electrophoretic gel image analysis (2001) M. S.R, 1 (1);Ye, X., Suen, C., Cheriet, M., Wang, E., A recent development in image analysis of electrophoresis gels (1999) Vision Interface 99, , Canada; 
Shadle, S., Allen, D., Guo, H., Pogozelski, W., Bashkin, J., Tullius, T., Quantitative analysis of electrophoresis data: Novel curve fitting methodology and its application to the determination of aprotein-DNA binding constant (1997) Nucleic Acids Research, 25 (4); 
Sousa, A., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic lane and band detection in images of thin layer chromatography (2004) Springer Lecture Notes on Computer Science, LNCS, 3212, pp. 372-380; 
Heijden, F., Duin, R., Ridder, D., Tax, D., (2004) Classification, Parameter Estimation and State Estimation, , John Wiley and Sons, England; 
Sewell, A., (1991) Urinary Oligosaccharides. Techniques in Diagnostic Human Biochemical Genetics, pp. 219-231. , Willey-Liss, Inc; 
Thomas, G., Disorders of Glycoprotein Degradation. α-Mahnosidosis, β-Mannosidosis, Fucosidosis and Sialidosis. Disorders of Glycoprotein Degradation, , Chap. 140; 
Durant, G., Seta, N., Protein glycosylation and diseases: Blood and urinary oligosaccharides as markers for diagnosis and therapeutic monitoring (2000) Clinical Chemistry, 46 (6), pp. 795-805; 
Marques De S{\'a}, J., (2003) Applied Statistics Using SPSS, STATISTICA and MATLAD, , Springer Verlag
},
	month = {2005///},
	pages = {974 - 981},
	address = {Toronto},
	abstract = {Thin-Layer Chromatography images are used to detect and identify the presence of specific oligosaccharides, expressed by the existence, at different positions, of bands in the gel image. ID gaussian deconvolution, commonly used for band detection, does not produce good results due to the large curvature observed in the bands. To overcome this uncertainty on the band position, we propose a novel feature extraction methodology that allows an accurate modeling of curved bands. The features are used to classify the data into two different classes, to differentiate normal from pathologic cases. The paper presents the developed methodology together with the analysis and discussion of the results. {\textcopyright} Springer-Verlag Berlin Heidelberg 2005.},
	keywords = {Edge detection, Feature extraction, Image analysis, Image segmentation, Oligosaccharides, Thin layer chromatography, Thin-Layer Chromatography images},
	isbn = {03029743 (ISSN); 3540290699 (ISBN); 9783540290698 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645996661\&partnerID=40\&md5=6ef97f4dfec52add55258aec9e98bb52},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A. and Aguiar, R. and S{\'a} Miranda, C.}
}
@article {2441,
	title = {Improving the adhesion of poly(ethylene terephthalate) fibers to poly(hydroxyethyl methacrylate) hydrogels by ozone treatment: Surface characterization and pull-out tests},
	journal = {PolymerPolymer},
	volume = {46},
	year = {2005},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: POLMAdoi: 10.1016/j.polymer.2005.08.033Language of Original Document: EnglishCorrespondence Address: Ferreira, L.; Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: lino@mit.eduReferences: Hart, B.L., Deyo, R.A., Cherkin, D.C., (1995) Spine, 20, pp. 11-19;Bao, Q.-B., McCullen, G.M., Higham, P.A., Dumbleton, J.H., Yuan, H.A., (1996) Biomaterials, 17, pp. 1157-1167; 
Martz, E.O., Goel, V.K., Pope, M.H., Park, J.B., (1997) J Biomed Mater Res (Appl Biomater), 38, pp. 267-288; 
Strobel, M., Walzak, M.J., Hill, J.M., Lin, A., Karbashewski, E., Lyons, C.S., (1995) J Adhes Sci Technol, 9 (3), pp. 365-383; 
Le, Q.T., Pireaux, J.J., Caudano, R., (1997) J Adhes Sci Technol, 11, pp. 735-751; 
O{\textquoteright}Hare, L.-A., Smith, J.A., Leadley, S.R., Parbhoo, B., Goodwin, A.J., Watts, J.F., (2002) Surf Interface Anal, 33, pp. 617-625; 
Walzak, M.J., Flynn, S., Foerch, R., Hil, J.M., Karbashewski, E., Lin, A., (1995) J Adhes Sci Technol, 9, pp. 1229-1248; 
Hill, J.M., Karbashewski, E., Lin, A., Strobel, M., Walzak, M.J., (1995) J Adhes Sci Technol, 9 (12), pp. 1575-1591; 
Blais, P., Day, M., Wiles, D.M., (1973) J Appl Polym Sci, 17, pp. 1895-1907; 
Day, M., Wiles, D.M., (1972) J Appl Polym Sci, 16, pp. 175-189; 
Nissen, K.E., Stevens, M.G., Stuart, B.H., Baker, A.T., (2001) J Polym Sci, Part B: Polym Phys, 39, pp. 623-633; 
Chen, W., McCarthy, T.J., (1998) Macromolecules, 31, pp. 3648-3655; 
Mougenot, P., Marchand-Brynaert, J., (1996) Macromolecules, 29, pp. 3552-3559; 
Hebeish, A., Shalaby, S.E., Bayazeed, A.M., (1981) J Appl Polym Sci, 26, pp. 3253-3269; 
Degert, C., Dupuy, B., Labat, B., Baquey, C., (1993) Biomat Art Cells Immobil Biotech, 21, pp. 553-561; 
Ambrosio, L., Netti, P.A., Iannace, S., Huang, S.J., Nicolais, L., (1996) J Mater Sci: Mater Int Med, 7, pp. 251-254; 
Rule, M., Physical constants of poly(oxyethylene-oxyterephthaloyl) (poly(ethylene terephthalate)) (1999) Polymer Handbook, pp. V/113-V/118. , J. Brandrup E.H. Immergut E.A. Grulke Wiley New York; 
Seah, M.P., Dench, W.A., (1979) Surf Interface Anal, 1, pp. 2-11; 
Strobel, M., Lyons, C., Strobel, J.M., Kapaun, R.S., (1992) J Adhes Sci Technol, 6 (4), pp. 429-443; 
Harrick, N.J., (1979) Internal Reflection Spectroscopy, , 2nd ed Wiley New York; 
Andrade, J.D., Smith, L.M., Gregonis, D.E., (1985) The Contact Angle and Interface Energetics, , Plenum press New York; 
Peeling, J., Clark, D.T., (1983) J Polym Sci: Polym Chem Ed, 21, pp. 2047-2055; 
Peeling, J., Courval, G., Jazzar, M.S., (1984) J Polym Sci: Polym Chem Ed, 22, pp. 419-428; 
Razumovskii, S.D., Kefeli, A.A., Zaikov, G.E., (1971) Eur Polym J, 7, pp. 275-285; 
Wang, Y., Lehmann, S., (1999) Appl Spectrosc, 53, pp. 914-918; 
Liang, C.Y., Krimm, S., (1959) J Mol Spectrosc, 3, pp. 554-574; 
Pavia, D.L., Lampman, G.M., Kriz, G.S., (1996) Introduction to Spectroscopy, , 2nd ed Harcourt Brace College Publishers New York; 
Carlsson, D.J., Wiles, D.M., (1969) Macromolecules, 2 (6), pp. 597-606; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies: Tables and Charts, , 3rd ed Wiley New York; 
Rabek, J.F., Lucki, J., R{\r a}nby, Y., Watanabe, Y., Qu, B.J., (1988) Photoozination of Polypropylene, , American Chemical Society Washington, DC; 
Ton-That, C., Teare, D.O.H., Campbell, P.A., Bradley, R.H., (1999) Surf Sci, 433-435, pp. 278-282; 
Tanaka, K., Minoshima, K., Grela, W., Komai, K., (2002) Compos Sci Technol, 62, pp. 2169-2177; 
Sadiku, E.R., Sanderson, R.D., (2001) Macromol Mater Eng, 286, pp. 535-545; 
Pisanova, E., Zhandarov, S., (2001) J Adhes, 75, pp. 89-127; 
Owens, D.K., (1975) J Appl Polym Sci, 19, pp. 3315-3326; 
Briggs, D., Rance, D.G., Kendall, C.R., Blythe, A.R., (1980) Polymer, 21, pp. 895-900; 
Fu, S.-Y., Yue, C.-Y., Hu, X., Mai, Y.-W., (2000) Compos Sci Technol, 60, pp. 569-579
},
	month = {2005///},
	pages = {9840 - 9850},
	abstract = {This work reports a methodology to improve the adhesion between poly(ethylene terephthalate) (PET) fibers and poly(hydroxyethyl methacrylate) (pHEMA) hydrogels by treating PET with ozone. The surface chemistry of PET was examined by water contact angle measurements, X-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRAS) and attenuated total reflectance infrared spectroscopy (ATR-IR) yielding information about the chemical functionalities at depths upon 0.6 μm. Ozone treatment introduces several polar groups in the surface of PET through oxidation and chain scission resulting in increased wettability. These groups include mostly carboxylic and anhydride groups and in small extent hydroxyl groups. Atomic force microscopy (AFM) analysis shows that the surface of ozone-treated PET films is fully covered with spherical particles that are removed after washing the film with water. During the washing step carboxylic functionalities were removed preferentially, as demonstrated by XPS and IR analysis. According to pull-out tests, PET monofilaments and bundles treated by ozone had a higher adhesion to pHEMA hydrogels than untreated ones. The apparent interfacial shear strength is 65\% higher on pHEMA hydrogel containing an ozonated than an untreated PET monofilament. In addition, the force to pull-out an ozone-treated PET bundle from pHEMA hydrogel is ca. 81\% higher than the one observed for the untreated bundle. {\textcopyright} 2005 Elsevier Ltd. All rights reserved.},
	keywords = {Adhesion, Atomic force microscopy, Contact angle, hydrogel, Hydrogels, infrared spectroscopy, Oxidation, Ozone, ozone treatment, Ozone treatments, Poly(ethylene terephthalate), polyethylene terephthalate fiber, Polyethylene terephthalates, Polymethyl methacrylates, pull-out, Pull-out tests, Surface characterization, Surface chemistry, surface property, X ray photoelectron spectroscopy},
	isbn = {00323861 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-26844540116\&partnerID=40\&md5=bebe6186e9e73c41648eedee7d5c0f61},
	author = {Ferreira, L. and Evangelista, M.B. and Martins, M.C.L. and Granja, P.L. and Esteves, J.L. and Barbosa, M.A.}
}
@conference {2442,
	title = {Neural network classification: Maximizing zero-error density},
	booktitle = {Lecture Notes in Computer ScienceLect. Notes Comput. Sci.},
	series = {Third International Conference on Advances in Patten Recognition, ICAPR 2005},
	volume = {3686},
	year = {2005},
	note = {Conference code: 65875Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Silva, L.M.; Instituto de Engenharia Biom{\'e}dica, Lab. de Sinai e Imagem Biom{\'e}dica, Campus da FEUP, Rua Dr. Roberto Frias, 4200 - 465 Porto, PortugalReferences: Erdogmus, D., Principe, J.C., An error-entropy minimization algorithm for supervised training of nonlinear adaptive systems (2002) IEEE Transactions on Signal Processing, 50 (7), pp. 1780-1786;Erdogmus, D., Principe, J., Generalized information potential criterion for adaptive system training (2002) IEEE Transactions on Neural Networks, 13 (5), pp. 1035~1044; 
Santos, J.M., Alexandre, L.A., Marques De S{\'a}, J., The error entropy minimization algorithm for neural network classification (2004) Int. Conf. on Recent Advances in Soft Computing, , Nottingham, United Kingdom; 
Santos, J.M., Alexandre, L.A., De Sa, J.M., Optimization of the error entropy minimization algorithm for neural network classification (2004) Intelligent Engineering Systems Through Artificial Neural Networks, 14, pp. 81-86. , C. Dagli, A. Buczak, D. Enke, M. Embrechts, and O. Ersoy, editors, ASME Press Series; 
Silva, L.M., De Sa, J.M., Alexandre, L.A., Neural network classification using shannon{\textquoteright}s entropy (2005) European Symposium on Artificial Neural Networks; 
Jacobs, R., Jordan, M., Nowlan, S., Hinton, G., Adaptive mixtures of local experts (1991) Neural Computation, 3, pp. 79-87; 
Blake, C.L., Merz, C.J., (1998) UCI Repository of Machine Learning Databases, , http://www.ics.uci.edu/~mlearn/MLRepository.html, University of California, Irvine, Dept. of Information and Computer Sciences
},
	month = {2005///},
	pages = {127 - 135},
	address = {Bath},
	abstract = {We propose a new cost function for neural network classification: the error density at the origin. This method provides a simple objective function that can be easily plugged in the usual backpropagation algorithm, giving a simple and efficient learning scheme. Experimental work shows the effectiveness and superiority of the proposed method when compared to the usual mean square error criteria in four well known datasets. {\textcopyright} Springer-Verlag Berlin Heidelberg 2005.},
	keywords = {Algorithms, Backpropagation, Backpropagation algorithm, Classification (of information), Costs, Error analysis, Function evaluation, Learning systems, Mean square error, Neural network classification, Neural networks, Zero-error density},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27244450344\&partnerID=40\&md5=73ee3c0c073fb3bc7bc85013bf7dcce6},
	author = {Silva, L.M. and Alexandre, L.A. and De S{\'a}, J.M.},
	editor = {Singh S. and Singh M. and Apte C. and Perner P.}
}
@article {2457,
	title = {Neural networks trained with the EEM algorithm: Tuning the smoothing parameter},
	journal = {WSEAS Transactions on SystemsWSEAS Trans. Syst.},
	volume = {4},
	year = {2005},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Santos, J.M.; Intituto de Engenharia Biom{\'e}dica, Porto, Portugal; email: jms@isep.ipp.ptReferences: Erdogmus, D., Principe, J., "An Error-Entropy Minimization Algorithm for Supervised Training of Nonlinear Adaptive Systems" (2002) Trans. On Signal Processing, 50 (7), pp. 1780-1786;Santos, J.M., Alexandre, L.A., Marques de S{\'a}, J., "The error entropy minimization algorithm for neural network classification" (2004) Proceedings of the 5th International Conference on Recent Advances in Soft Computing, pp. 92-97. , A. Lofti, Ed. Nottingham Trent University; 
Santos, J.M., Marques de S{\'a}, J., Alexandre, L.A., Sereno, F., "Optimization of the error entropy minimization algorithm for neural network classification" (2004) Intelligent Engineering Systems Through Artificial Neural Networks, 14, pp. 81-86. , C.H. Dagli, A.L. Buczak, D.L. Enke, M.J. Embrechts, and O. Ersoy, Eds., ASME Press; 
Erdogmus, D., Principe, J., "Entropy Minimization Algorithm for Neural Networks" (2001) Proceedings of the Intl. Joint Conf. on Neural Networks, pp. 3003-3008; 
Xu, D., Princpe, J., "Training mlps layer-by-layer with the information potential" (1999) Intl. Joint Conf. on Neural Networks, pp. 1716-1720; 
Silverman, B.W., (1986) Density Estimation for Statistics and Data Analisys, 26. , Chapman \& Hall; 
Bowman, A.W., Azzalini, A., (1997) Applied Smooting Techniques for Data Analysis, , London: Oxford University Press; 
Jacobs, R., Jordan, M., Nowlan, S., Hinton, G., "Adaptive mixtures of local experts" (1991) Neural Computation, pp. 79-87
},
	month = {2005///},
	pages = {295 - 299},
	abstract = {The training of Neural Networks and particularly Multi-Layer Perceptrons (MLP{\textquoteright}s) is made by minimizing an error function usually known as "cost function". In our previous works, we apply the Error Entropy Minimization (EEM) algorithm in classification and its optimized version using, as cost function, the entropy of the errors between the outputs and the desired targets of the neural network. One of the difficulties in implementing the EEM algorithm is the choice of the smoothing parameter, also known as window size, in the Parzen Window probability density function estimation for the computation of the entropy and its gradient. We present here a formula yielding the value of the smoothing parameter, depending on the number of data samples and on the neural network output dimension. Several experiments with real data sets were made in order to show the validity of the proposed formula.},
	keywords = {Cost function, entropy, Error entropy minimization (EEM) algorithm, Learning algorithms, Learning systems, Neural networks, Optimization, Parzen, Probability density function, Smoothing parameter},
	isbn = {11092777 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-21844450579\&partnerID=40\&md5=31c652daee111a68194cd4f006d7ca7b},
	author = {Santos, J.M. and Marques de S{\'a}, J. and Alexandre, L.A.}
}
@book {2424,
	title = {Porous HA scaffolds for drug releasing},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB, Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Queiroz, A.C., (2001) Biomaterials, 22, p. 1393},
	month = {2005///},
	pages = {407 - 410},
	abstract = {Dense and porous hydroxyapatite materials aimed at being used in controlled drug delivery, were characterised and studied in order to evaluate their ability to adsorb and release sodium ampicillin in a controlled manner, as a model for a drug delivery system, potentially applicable associated to surgery for the treatment of bone defects. These porous materials should also have adequate mechanical strength to withstand manipulation and sculpturing in surgery room. Adsorption and release profiles were obtained for a range of porous materials, leading to higher adsorption rates and more adequate release profiles than for dense materials.},
	keywords = {Adsorption, ampicillin, Controlled drug delivery, Drug release, hydroxyapatite, orthopedics, Porous hydroxyapatite, Porous hydroxyapatites, Porous materials, Scaffolds, Sodium ampicillin, Surgery, Surgery rooms},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34248995543\&partnerID=40\&md5=db9f063f6d3b98c88a9d87c693ada572},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J.}
}
@conference {2446,
	title = {Spectral methods in image segmentation: A combined approach},
	booktitle = {Lecture Notes in Computer ScienceLect. Notes Comput. Sci.},
	series = {Second Iberian Conference on Pattern Recognition and Image Analysis, IbPRIA 2005},
	volume = {3523},
	year = {2005},
	note = {Conference code: 65649Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Monteiro, F.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Campus de Santa Apol{\'o}nia, Apartado 134, 5301-857 Bragan{\c c}a, Portugal; email: monteiro@ipb.ptReferences: Fowlkes, C., Belongie, S., Chung, F., Malik, J., Spectral grouping using nystr{\"o}m method (2004) Trans. on Pattern Analysis and Machine Intelligence, 26 (2), pp. 214-225;Malik, J., Belongie, S., Leung, T., Shi, T., Contour and texture analysis for image segmentation (2001) International Journal of Computer Vision, 43 (1), pp. 7-27; 
Martin, D., Fowlkes, C., Tal, D., Malik, J., A database of human segmented natural images and its application to evaluating segmentation algorithms and measuring ecological statistics (2001) IEEE Int. Conf. on Computer Vision, 2, pp. 416-424; 
Meila, M., Shi, J., Learning segmentation by random walks (2000) Neural Information Processing Systems, pp. 873-879; 
Ng, A., Jordan, M., Weiss, Y., On spectral clustering: Analysis and an algorithm (2002) Advances in Neural Information Processing Systems, 14, pp. 849-856; 
Scott, D.S., Solving sparse symmetric generalized eigenvalue without factorization (1981) SIAM Journal of Numerical Analisys, 18, pp. 102-110. , http://www.netlib.org/laso/snlaso.f; 
Scott, G.L., Longuet-Higgins, H.C., Feature grouping by "relocalisation" of eigenvectors of the proximity matrix (1990) Proc. British Machine Vision Conf., pp. 103-108; 
Shi, J., Malik, J., Normalized cuts and image segmentation (2000) IEEE Trans. on Pattern Analysis and Machine Intelligence, 22 (8), pp. 888-905; 
Weiss, Y., Segmentation using eigenvectors: A unifying view (1999) International Conference on Computer Vision, pp. 975-982; 
Wertheimer, M., Laws of organization in perceptual forms (1938) A Sourcebook of Gestalt Psychology, pp. 71-88. , W. B. Ellis Editor; 
Wu, Z., Leahy, R., An optimal graph theoretic approach to data clustering: Theory and its application to image segmentation (1993) IEEE Trans. on Pattern Analysis and Machine Intelligence, 15, pp. 1101-1113; 
Yu, S., Shi, J., Multiclass spectral clustering (2003) International Conference on Computer Vision, pp. 313-319. , Nice, France
},
	month = {2005///},
	pages = {191 - 198},
	address = {Estoril},
	abstract = {Grouping and segmentation of images remains a challenging problem in computer vision. Recently, a number of authors have demonstrated a good performance on this task using spectral methods that are based on the eigensolution of a similarity matrix. In this paper, we implement a variation of the existing methods that combines aspects from several of the best-known eigenvector segmentation algorithms to produce a discrete optimal solution of the relaxed continuous eigensolution. {\textcopyright} Springer-Verlag Berlin Heidelberg 2005.},
	keywords = {Algorithms, Computer vision, Discrete optimal solution, Eigensolution, Eigenvalues and eigenfunctions, Image segmentation, Matrix algebra, Problem solving, Similarity matrix, Spectral methods},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-25144437374\&partnerID=40\&md5=5b6351af4ac58a16c41b5558a48ae9e2},
	author = {Monteiro, F.C. and Campilho, A.C.},
	editor = {Marques J.S. and Perez de la Blanca N. and Pina P.}
}
@article {2440,
	title = {Synthesis and characterization of new injectable and degradable dextran-based hydrogels},
	journal = {PolymerPolymer},
	volume = {46},
	year = {2005},
	note = {Cited By (since 1996): 49Export Date: 21 November 2012Source: ScopusCODEN: POLMAdoi: 10.1016/j.polymer.2005.07.089Language of Original Document: EnglishCorrespondence Address: Maia, J.; Departamento de Engenharia Qu{\'\i}mica, Universidade de Coimbra, Pinhal de Marrocos, 3030 Coimbra, Portugal; email: joao.rm.silva@networkcontacto.comReferences: Ferreira, L., Carvalho, R., Gil, M.H., Dordick, J.S., (2002) Biomacromolecules, 3, p. 333;Ferreira, L., Gil, M.H., Dordick, J.S., (2002) Biomaterials, 23, p. 3957; 
Ferreira, L., Rafael, A., Lamghari, M., Barbosa, M.A., Gil, M.H., Cabrita, A.M.S., (2004) J Biomed Mater Res, 68, p. 584; 
Lee, K.Y., Mooney, D.J., (2001) Chem Rev, 101 (7), p. 1869; 
Peppas, N.A., Bures, P., Leobandung, W., Ichikawa, H., (2000) Eur J Pharma Biopharm, 50, p. 27; 
Wong, W.H., Mooney, D.J., (1997) Synthetic Biodegradable Polymer Scaffolds, pp. 49-82. , A. Atala D.J. Mooney Birkh{\"a}user Boston; 
Mehvar, R., (2000) J Controlled Release, 69, p. 1; 
Nguyen, K.T., West, J.L., (2002) Biomaterials, 23, p. 4307; 
Liu, V.A., Bhatia, S.N., (2002) Biomed Microdevices, 4 (4), p. 257; 
Williams, C.G., Malik, A.N., Kim, T.K., Manson, P.N., Elisseeff, J.H., (2005) Biomaterials, 26, p. 1211; 
Bouhadir, K.H., Hausman, D.S., Mooney, D.J., (1999) Polymer, 40, p. 3575; 
Lee, K.Y., Alsberg, E., Mooney, D.J., (2001) J Biomed Mater Res, 56, p. 228; 
Lee, K.Y., Bouhadir, K.M., Mooney, D.J., (2000) Macromolecules, 33, p. 97; 
Kim, S.-H., Chu, C.C., (2000) J Biomed Mater Res, Appl Biomater, 53, p. 258; 
Meyvis, T., De Smedt, S., Stubbe, B., Hennink, W.E., Demeester, J., (2001) Pharm Res, 18 (11), p. 1593; 
Dziubla, T.D., Torjman, M.C., Joseph, J.I., Murphy-Tatum, M., Lowman, A.M., (2001) Biomaterials, 22, p. 2893; 
Jen, A.C., Wake, M.C., Mikos, A.G., (1996) Biotechnol Bioeng, 50, p. 357; 
Schneerson, R., Barrera, O., Sutton, A., Robbis, J.B., (1980) J Exp Med, 152, p. 361; 
Jia, X., Burdick, J.A., Kobler, J., Clifton, R.J., Rosowski, J.J., Zeitels, S.M., (2004) Macromolecules, 37, p. 3239; 
Edwards-L{\'e}vy, F., Andry, M.C., L{\'e}vy, M.C., (1993) Int J Pharm, 96, p. 85; 
Meyvis, T.K.L., De Smedt, S.C., Demeester, J., Hennin, W.E., (1999) J Rheol, 43, p. 933; 
Behravesh, E., Jo, S., Zygourakis, K., Mikos, A.G., (2002) Biomacromolecules, 3, p. 374; 
Washburn, E.W., (1921) Proc Natl Acad Sci, 7, p. 115; 
Ishak, M.F., Painter, T.J., (1978) Carbohyd Res, 64, p. 189; 
Tiziani, S., Sussich, F., Ces{\'a}ro, A., (2003) Carbohyd Res, 338, p. 1083; 
Van Dijk-Wolthuis, W.N.E., Franssen, O., Talsma, H., Van Steenbergen, M.J., Kettenes-Van Den Bosch, J.J., Hennink, W.E., (1995) Macromolecules, 28, p. 6317; 
Yu, R.J., Bishop, C.T., (1967) Can J Chem, 45, p. 2195; 
Domb, A.J., Linden, G., Polacheck, I., Benita, S., (1996) J Polym Sci, Part A: Polym Chem, 34, p. 1229; 
Pavia, D.L., Lampma, G.M., Kriz, G.S., (1996) Introduction to Spectroscopy, , 2nd ed Saunders College Publishing London; 
Vervoort, L., Vinckier, I., Moldenaers, P., Van Den Mooter, G., Augustijns, P., Kinget, R., (1999) J Pharm Sci, 88, p. 209; 
Smedt, S.C.D., Lauwers, A., Demeester, J., Van Steenbergen, M.J., Hennink, W.E., Roefs, S.P.F.M., (1995) Macromolecules, 28, p. 5082; 
Bell, C.L., Peppas, N.A., (1995) Adv Polym Sci, 122, p. 125; 
Van Dijk-Wolthuis, W.N.E., Hoogeboom, J.A.M., Van Steenbergen, M.J., Tsang, S.K.Y., Hennink, W.E., (1997) Macromolecules, 30, p. 4639; 
Dawlee, S., Sugandhi, A., Balakrishnan, B., Labarre, D., Jayakrishnan, A., (2005) Biomacromolecules, 6, p. 2040; 
Peppas, N.A., Hydrogels (1996) Biomaterials Science: An Introduction to Materials in Medicine, p. 60. , B.D. Ratner A.S. Hoffman F.J. Schoen J.E. Lemons Academic Press London; 
Pirard, R., Rigacci, A., Mar{\'e}chal, J.C., Quenard, D., Chevalier, B., Achard, P., (2003) Polymer, 44, p. 4881; 
Ferreira, L., Gil, M.H., Cabrita, A.M.S., Dordick, J.S., (2005) Biomaterials, 26, p. 4707
},
	month = {2005///},
	pages = {9604 - 9614},
	abstract = {Injectable and degradable hydrogels are very interesting networks for drug delivery and cell transplantation applications since they can be administered in the human body in a minimally invasive way. In most cases, the crosslinking reaction occurs by photopolymerisation or free radical polymerisation; however, the use of chemical initiators may promote cell death. In the current work, injectable and degradable dextran-based hydrogels were prepared without the use of initiators. Dextran, a natural glucose-containing polysaccharide, was oxidized with sodium periodate (dexOx) and the derivatives characterized by NMR and FTIR spectroscopy{\textquoteright}s as well as by colorimetric techniques. The oxidized derivatives were crosslinked with adipic acid dihydrazide (AAD), forming a gel within 2-4 min. The obtained hydrogels were characterized by their mechanical properties, swelling and degradation behavior under physiologic conditions. In addition, the hydrogel interior morphology as well as porous structure was evaluated by scanning electron microscopy (SEM) and mercury intrusion porosimetry (MIP). MIP analysis showed that dexOx hydrogels crosslinked with 10\% of AAD were macroporous with pore sizes ranging from 0.32 to 0.08 μm. As expected, the average pore size increased during hydrogel degradation as confirmed by SEM and MIP studies. {\textcopyright} 2005 Elsevier Ltd. All rights reserved.},
	keywords = {Cell Death, Cell transplantation, Colorimetry, Crosslinking, Degradation, Drug delivery, Drug dosage, Fourier transform infrared spectroscopy, Free radical polymerization, hydrogel, Hydrogels, Mercury intrusion porosimetry (MIP), morphology, Nuclear magnetic resonance spectroscopy, Photopolymerization, Polysaccharides, Pore size, Porosimeters, Scanning electron microscopy, Synthesis (chemical)},
	isbn = {00323861 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-26844528911\&partnerID=40\&md5=134ba47873a69e30382b727af0d99863},
	author = {Maia, J. and Ferreira, L. and Carvalho, R. and Ramos, M.A. and Gil, M.H.}
}
@article {2431,
	title = {TiO                    2 type influences fibronectin adsorption
},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {16},
	year = {2005},
	note = {Cited By (since 1996): 28Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-005-4725-4Language of Original Document: EnglishCorrespondence Address: Sousa, S.R.; INEB, Instituto de Engenharia Biom{\'e}dica, Lab. Biomateriais, R. do Campo Alegre, 823, 4150-180 Porto, Portugal; email: ssousa@ibmc.up.ptChemicals/CAS: fibronectin, 86088-83-7; titanium dioxide, 1317-70-0, 1317-80-2, 13463-67-7, 51745-87-0; titanium, 7440-32-6; hydrogen peroxide, 7722-84-1; iodine 125, 14158-31-7, 22822-81-7; Fibronectins; Solutions; titanium dioxide, 13463-67-7; Titanium, 7440-32-6References: Horbett, T.A., (1994) Colloids and Surfaces B: Biointerfaces, 2, p. 225;Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., (2005) J Biomed Mater Res., 75 A, p. 387; 
Johnson, K.J., Sage, H., Briscoe, G., Erickson, H.P., (1999) J. Biol. Chem., 274, p. 15473; 
Bergkvist, M., Carlsson, J., Oscarsson, S., (2003) J. Biomed. Mater. Res. Part A., 64 A, p. 349; 
Ugarova, T.P., Zamarron, C., Veklich, Y., Bowditch, R.D., Ginsberg, M.H., Weisel, J.W., Plow, E.F., (1995) Biochemistry, 34, p. 4457; 
Sukenik, C.N., Balachander, N., Culp, L.A., Lewandowska, K., Merritt, K., (1990) J. Biomed. Mater. Res., 24, p. 1307; 
Sousa, S.R., Moradas- Ferreira, P., Melo, L.V., Saramago, B., Barbosa, M.A., (2004) Langmuir, 20, p. 9745; 
Gehanno, V., Freitas, P.P., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Soares, J.C., Silva, M.F., (1999) IEEE Trans. Magn., 35, p. 4361; 
(2003) Iodine-125, A Guide to Radioiodination Techniques - Amersham Life Science, p. 64. , Amersham International plc, Amersham Place, Little Chalfont, Bucks, HP7 9NA, England, 199; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., (2001) J. Biomed. Mater. Res., 55, p. 45; 
Yu, X.J., Brash, J.L., (1993) Test Procedures for the Blood Compatibility of Biomaterials, Dordrecht, p. 287. , edited by S. Dawids (Kluwer Academic Publishers, Dordrecht); 
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., (1985) Analytical Biochemistry, 150, p. 76; 
Lindberg, B., Maripuu, R., Siegbahn, K., Larsson, R., Golander, C.G., Eriksson, J.C., (1983) J. Colloid Interface Sci., 95, p. 308; 
Auditore, A., Satriano, C., Coscia, U., Ambrosone, G., Parisi, V., Marletta, G., (2002) Biomolecular Engineering, 19, p. 85; 
Miller, R., Treppo, S., Voigt, A., Zingg, W., Neumann, A.W., (1993) Colloids and Surfaces, 69, p. 203; 
Serro, A.P.V., Fernandes, A.C., Saramago, B.D.V., (1997) Colloids and Surfaces A-Physicochemical and Engineering Aspects, 125, p. 209; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., (1997) Langmuir, 13, p. 3404; 
Matsuura, T., Hosokawa, R., Okamoto, K., Kimoto, T., Akagawa, Y., (2000) Biomaterials, 21, p. 1121; 
Ferraz, M.P., Monteiro, F.J., Serro, A.P., Saramago, B., Gibson, I.R., Santos, J.D., (2001) Biomaterials, 22, p. 3105; 
Do Serro, A., Fernandes, A.C., Saramago, B.D.V., Norde, W., (1999) J. Biomed. Mater. Res., 46, p. 376; 
Norde, W., Anusiem, A.C.I., (1992) Colloids and Surfaces, 66, p. 73; 
Rouxhet, L., Duhoux, F., Borecky, O., Legras, R., Schneider, Y.J., (1998) Journal of Biomaterials Science-Polymer Edition, 9, p. 1279; 
Norde, W., (1986) Advances in Colloid and Interface Science, 25, p. 267; 
Tamada, Y., Ikada, Y., (1993) J. Colloid Interface Sci., 155, p. 334
},
	month = {2005///},
	pages = {1173 - 1178},
	abstract = {Human fibronectin (FN) plays a key role in the biointegration of implants as the success depends on adsorption of proteins like FN [1]. Indeed FN can be an intermediary between the biomaterial surface and cells. The adsorption of human fibronectin (FN) on commercially pure titanium with a titanium oxide layer formed in a H                        2O
                        2 solution (TiO
                        2 cp) and TiO
                        2 sputtered on Si (TiO
                        2 sp) was studied. Adsorption isotherms and the work of adhesion were assessed by wettability studies, X-ray photoelectron spectroscopy (XPS), and by radiolabelling of FN with 
                        125I, 
                        125I-FN. Exchangeability of bound FN by free FN, was also evaluated by the radiolabelling technique. Contact angle determinations have shown that FN displays higher affinity for the TiO
                        2 cp surface than for the TiO
                        2 sp. As expected from the surface free energy values, the work of adhesion of FN is higher for the TiO
                        2 cp substrate, the more hydrophilic one, and lower for the TiO
                        2 sp substrate, the more hydrophobic one. The adsorption isotherms were evaluated by two different techniques: radiolabelling of FN (
                        125I-FN) and XPS. TiO
                        2 cp adsorbs more FN than the TiO
                        2 sp surfaces as shown by the radiolabelling data. FN molecules are also more strongly attached to the former surface as indicated by the work of adhesion and by the exchangeability studies. Results using 
                        125I-FN also suggests that FN adsorbs as a multilayer for FN concentrations in solution higher than 100 μg/mL. {\textcopyright} 2005 Springer Science + Business Media, Inc.
},
	keywords = {Adsorption, adsorption kinetics, article, binding affinity, Biointegration, Biomaterials, Cells, chemistry, conference paper, Contact angles, Fibronectin, Fibronectins, human, Human fibronectin (FN), Humans, hydrogen peroxide, hydrophilicity, hydrophobicity, Implants (surgical), iodine 125, isotherm, Isotherms, isotope labeling, physical chemistry, priority journal, protein binding, Proteins, Radiolabelling, solution and solubility, Solutions, surface property, Titanium, Titanium dioxide, wettability, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-29144439699\&partnerID=40\&md5=f98f8448d034d8f8627d752516e3afbd},
	author = {Sousa, S.R. and Moradas-Ferreira, P. and Barbosa, M.A.}
}
@conference {2463,
	title = {Automatic delimitation of lung fields on chest radiographs},
	booktitle = {2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to NanoIEEE Int. Symp. Biomed. Imaging Macro Nano},
	series = {2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano},
	volume = {2},
	year = {2004},
	note = {Conference code: 64593Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Mendon{\c c}a, A.M.; Inst. de Engenharia Biom{\'e}dica, Lab. de Sinai e Imagem Biomedica, Campus da FEUP, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, PortugalReferences: Tsujii, O., Freedman, M., Mun, S., Automated segmentation of anatomic regions in chest radiographs using an adaptive-sized hybrid neural network (1998) Medical Physics, 25, pp. 998-1007;Armato III, S., Giger, M., MacMahon, H., Computerized detection of abnormal asymetry in digital lateral chest radiographs (1994) Medical Physics, 21, pp. 1761-1768; 
Armato III, S., Giger, M., Ashizawa, K., MacMahon, H., Automated lung segmentation in digital lateral chest radiographs (1998) Medical Physics, 25, pp. 1507-1520; 
Carrascal, F., Carreira, J., Souto, M., Automatic calculation of total lung capacity from automatically traced lung boundaries in posterior-anterior and lateral digital chest radiographs (1998) Medical Physics, 25, pp. 1118-1131; 
Ginneken, B., Romeny, B., Automatic segmentation of lung fields in chest radiographs (2000) Medical Physics, 27, pp. 2445-2455; 
Li, L., Zheng, Y., Kallergi, M., Clark, R., Improved method for automatic identification of lung regions on chest radiographs (2001) Academic Radiology, 8, pp. 629-638; 
Monteiro, M., Mendon{\c c}a, A.M., Campilho, A., Lung field detection on chest radiographs (2002) Proceedings of VIIP02, IASTED International Conference on Visualization, Imaging and Image Processing, pp. 367-372. , J. Villanueva (eds.), Spain
Sponsors: IEEE},
	month = {2004///},
	pages = {1287 - 1290},
	address = {Arlington, VA},
	abstract = {The purpose of the research herein presented is the automatic delimitation of lung fields in posterior-anterior digital chest radiographs. In a computer-aided diagnosis system the precise location of the lungs is important as it allows the reduction of the region under analysis, decreasing the computation time and facilitating data compression. Furthermore, it allows the delimitation of the search area, easing the selective tuning of the abnormalities detection algorithms. The results produced by the automatic method were validated by comparison with manual contours traced by experienced radiologists. Two programs with friendly interfaces were developed for this purpose. {\textcopyright} 2004 IEEE.},
	keywords = {Algorithms, Chest radiographs, Computer aided diagnosis, Contour detection, Data compression, Edge detection, Image segmentation, Lung fields, Pulmonary diseases, Radiography, X ray analysis, X ray imaging},
	isbn = {0780383885 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17144373368\&partnerID=40\&md5=4f13427d02a5013364f4d761ccf5a7b8},
	author = {Mendon{\c c}a, A.M. and Da Silva, J.A. and Campilho, A.}
}
@book {2465,
	title = {Automatic lane and band detection in images of thin layer chromatography},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {3212},
	year = {2004},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Sousa, A.V.; Institute de Engenharia Biom{\'e}dica, Rua Roberto Prias, 4200-465 Porto, Portugal; email: ats@isep.ipp.ptReferences: Sewell, A., (1991) Urinary Oligosaccharides. Techniques in Diagnostic Human Biochemical Genetics, pp. 219-231. , Willey-Liss, Inc;Thomas, G., Disorders of Glycoprotein Degradation. α-Mannosidosis, β-Mannosidosis, Fucosidosis and Sialidosis, , Disorders of Glycoprotein Degradation (Chap. 140); 
Starr, C., Masada, R., Hague, C., Klock, J., Fluorophore-assisted Carbohydrate Electrophoresis in the Separation, Analysis, and Sequencing of Carbohydrates (1996) Journal of Chromatography A, 720, pp. 295-321; 
Klein, A., Lebreton, A., Lemoine, J., P{\'e}rini, J., Roussel, P., Michalski, J., Identification of Urinary Oligosaccharides by Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (1998) Clinical Chemistry, 44 (12), pp. 2422-2428; 
Dempster, A., Laird, N., Rubin, D., Maximum Likelihood from Incomplete Data via the em Algorithm (1977) Journal of the Royal Statistical Society, B, 39 (1), pp. 1-38; 
Schwedt, G., The Essential Guide to Analytical Chemistry, , John Wiley \& Sons; 
Dennis, J., Nonlinear Least-squares, State of the Art in Numerical Analysis, , D. Jacobs Academic Press; 
Byrd, R., Schnabel, R., Shultz, G., Approximate Solution of the Trust Region Problem by Minimization over Two-Dimensional Subspaces (1988) Mathematical Programming, 40, pp. 247-263; 
Branch, M., Coleman, T., Li, Y., A Subspace, Interior, and Conjugate Gradient Method for Large-Scale Bound-Constrained Minimization Problems (1999) SIAM SISC, 21 (1), pp. 1-23; 
Nabney, I., Netlab Algorithms for Pattern Recognition (2003) Advances in Pattern Recognition, , Springer; 
Marques, J., Reconhecimento de Padr{\~o}es, , M{\'e}todos Estatisticos e Neuronais, IST-Press; 
Machado, A., Campos, M., Siqueira, A., Carvalho, O., (1997) An Iterative Algorithm for Segmenting Lanes in Gel Electrophoresis Images; 
},
	month = {2004///},
	pages = {158 - 165},
	abstract = {This work aims at developing an automatic method for the analysis of TLC images for measuring a set of features that can be used for the characterization of the distinctive patterns that result from the separation of oligosaccharides contained in human urine. This paper describes the methods developed for the automatic detection of the lanes contained in TLC images, and for the automatic separation of bands for each detected lane. The extraction of quantitative information related with each band was accomplished with two methods: the EM expectation-maximization and nonlinear least squares trustregion algorithms. The results of these methods, as well as additional quantitative information related with each band, are also presented. {\textcopyright} Springer-Verlag 2004.},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645961076\&partnerID=40\&md5=ef452eea389056fa97b81d62d8f451b9},
	author = {Sousa, A.V. and Aguiar, R. and Mendonca, A.M. and Campilho, A.}
}
@book {2488,
	title = {Biological Activity of Two Glass Ceramics in the Meta- and Pyrophosphate Region: A Comparative Study},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Dias, A.G.; Faculdade de Engenharia, Universidade do Porto, DEMM, Rua Dr. Roberta Frias, 4200 Porto, PortugalReferences: Dias, A.G., Lopes, M.A., Gibson, I., Santos, J.D., J. Non-Crystal. Solids, , in press;Dias, A.G., Lopes, M.A., Tsuru, K., Hayakawa, S., Santos, J.D., Osaka, A., Physiscs and Chemistry of Glasses, , in press; 
Dias, A.G., Lopes, M.A., Santos, J.D., Advance Materials Science, , in press; 
Rykke, M., Rolla, G., Sonju, T., (1988) Scand. J. Dent. Res., 96, pp. 517-522
},
	month = {2004///},
	pages = {825 - 828},
	address = {Porto},
	abstract = {Based upon the CaO-P                        2O
                        5 glass system, two glass ceramics were prepared in the metaand pyrophosphate regions. The present work describes preliminary results concerning the biological activity of MK5B (45CaO-45P
                        2O
                        5-5MgO-5K20, in mol \%) and MT13B (45CaO-37P
                        2O
                        55-5MgO-13TiO
                        2, in mol \%) using MG63 osteoblast-like cells. Both materials supported cell growth and proliferation which increased with the incubation time. However, cell growth was significantly lower on seeded MK5B samples. SEM observation showed that cell adhesion and spreading were hampered on this material. Evident degradation of the material surface was observed with simultaneous cell proliferation and material precipitation. By contrast, MT13B presented a relatively stable surface throughout the culture time. Results suggest that the in vitro biological behaviour of MK5B and MT13B is related with differences in the degradation rates of the two materials during the culture time.
},
	keywords = {Adhesion, Biodegradable glass ceramics, Biodegradation, Bone, cell proliferation, Cells, Crystallization, Degradation, Glass ceramics, incubation time, MG63 osteoblast-like cells, Scanning electron microscopy, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0348146001\&partnerID=40\&md5=5245524d4dfb5615946aadcf5eb7c37a},
	author = {Dias, A.G. and Costa, M.A. and Lopes, M.A. and Santos, J.D. and Fernandas, M.H.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2486,
	title = {CaO-P                    2O
                    5 Glass-Hydroxyapatite Thin Films Obtained by Laser Ablation: Characterisation and In Vitro Bioactivity Evaluation
},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: mpferraz@ufp.ptReferences: Arias, J.L., (1997) J Mater. Sci. Mater. Med., 8, pp. 873-876;Peraire, C., (1997) 13 th European Conference on Biomaterials, p. 75. , G{\"o}teborg, Sweden, 4-7 Sept; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., Fernandes, M.H., Trigo-Cabral, A., Santos, J.D., Monteiro, F.J., (1999) J. Mat Sci: Mat. Med, 10 (9), p. 567
},
	month = {2004///},
	pages = {347 - 350},
	address = {Porto},
	abstract = {Hydroxyapatite (HA) coatings have been applied to improve adhesion of non-cemented implants to host bone. Plasma spraying is the most common technique leading to thick calcium phosphate films (\&gt;120μm). Pulse laser deposition (PLD), is a possible alternative method to obtain thin (\&lt;10 μm), well adherent hydroxyapatite (HA) films. Similarly to synthetic HA, biological apatites contain Ca                        2+, PO
                        4
                        3- and OH
},
	keywords = {2O, 5 glass, Adhesion, Bioactivity, Bone, Calcium Compounds, CaO-P, Coatings, Deposition, Energy dispersive spectroscopy, Homogeneous mixtures, Hydroxylapatite thin films, Implants (surgical), In vitro bioactivity, Laser ablation, Plasma spraying, Scanning electron microscopy, Thin films, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516195\&partnerID=40\&md5=ddd47b73956669a35aa9041ce9102612},
	author = {Ferraz, M.P. and Monteiro, F.J. and Gi{\~a}o, D. and Leon, B. and Gonzalez, P. and Liste, S. and Serra, J. and Arias, J. and Perez-Amor, M.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2494,
	title = {Combining Foaming and Starch Consolidation Methods to Develop Macroporous Hydroxyapatite Implants},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 10Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Lemos, A.F.; Depto. de Engenharia Cer{\^a}mica, CICEO, Campus de Santiago, 3810-193 Aveiro, Portugal; email: ilemos@cv.ua.ptReferences: De Groot, K., (1983) Bioceramics of Calcium Phosphates, , CRC Press, Boca Raton, FL;Hench, L.L., Wilson, J., (1993) An Introduction to Bioceramics, , World Scientific, London, UK; 
Ohgushi, H., Okumura, M., Yoshikawa, T., Inoue, K., Senpuku, N., Tamai, S., Shors, E.C., (1992) J. Biomed. Mater. Res., 26, p. 885; 
Minnear, W.P., (1992) Processing of Foamed Ceramics, Ceramic Transactions, Forming Science and Technology for Ceramics Vol. 26, 26. , ed. M.J. Cima, The American Ceramic Society, USA; 
Omatete, O., Janney, M.A., Strehlow, R.A., (1991) Am. Ceram Soc.Bull., 70, p. 1641; 
Kosmac, T., Novak, S., Sajko, M., (1995) Fourth Euro Ceramics, Vol. 1, Basic Science Developments, 1. , ed. C. Galassi. Gruppo Editoriale Faenza Editrice S.p.a., Italy; 
Graule, T.J., Baader, F.H., Gauckler, L.J., (1994) Cfi/Ber. DKH., 71, p. 317; 
Novich, B.E., Sundback, C.A., Adams, R.W., (1992) Ceramic Transactions, 26. , ed. M. J. Cima. American Ceramic Society, USA; 
Rives, R.D., U. S. Patent 4113480 (1976)Lycfeldt, O., Brandt, J., Lesca, S., (2000) J. Eur. Ceram. Soc., 20 (14-15), p. 2551; 
Olhero, S.M., Tar{\`\i}, G., Coimbra, M.A., Ferreira, J.M.F., (2000) J. Eur. Ceram. Soc., 20 (4), p. 423; 
Lycfeldt, O., Ferreira, J.M.F., (1998) J. Eur. Ceram. Soc., 18, p. 131; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., (2003) Advanced Materials Forum, , in press
},
	month = {2004///},
	pages = {1041 - 1044},
	address = {Porto},
	abstract = {Hydroxyapatite (HA) is the calcium-phosphate material with composition closest to that of bone, what makes it suitable for osseous implant purposes. This material was used to produce macroporous structures with pores larger than 100 μm, which are believed to be suitable for allowing bone ingrowth. The macroporous structures were generated and consolidated by combining foaming and starch consolidation methods. The porous structures could be tailored according to the final application by varying the proportion of different foaming agents, foam-bath concentrate (FBC) and sodium lauril sulphate (SLS). Playing with these proportions it was also possible to improve foam stability and model the size of pores and pore interconnections in order to reproduce the pore structure of natural bone.},
	keywords = {Bioceramics, Biocompatibility, Bone, Composition, Foaming method, hydroxyapatite, Hydroxyapatlte, Implants (surgical), Macroporous structures, Microstructure, Pore size, Porous ceramics, Porous materials, Starch, Starch consolidation},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346885485\&partnerID=40\&md5=63e5b33a0a2185af44b629c565e5240f},
	author = {Lemos, A.F. and Ferreira, J.M.F.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2495,
	title = {Designing of Bioceramics with Bonelike Structures Tailored for Different Orthopaedic Applications},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Lemos, A.F.; Depto. de Engenharia Cer{\^a}mica, CICECO, Campus de Santiago, 3810-193 Aveiro, Portugal; email: ilemos@cv.ua.ptReferences: Hing, K.A., Best, S.M., Tanner, K.E., Bondfield, W., Revell, P.A., (1997) J. Mater. Sci: Mater Med., 8, p. 731;Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Kon, E., Muraglia, A., Corsi, A., Bianco, P., Marcacci, M., Martin, I., Boyde, A., Quarto, R., (2000) J. Biomed. Mater. Res., 49 (3), p. 328; 
Prado da Silva, M.H., Lemos, A.F., Lopes, M.A., Ferreira, J.M.F., Santos, J.D., (2002) Journal of Non-crystalline Solids, 304, p. 286; 
Schwartzwalder, K., Somers, A.V., U.S. Patent n{\textdegree} 3 090 094, (1963)Saggio-Woyanski, J., Scott, C.E., Minnear, W.P., (1992) Amer. Ceram. Soc. Bull., 71 (11), p. 1674; 
Olhero, S.M., Tar{\`\i}, G., Coimbra, M.A., Ferreira, J.M.F., (2000) J. Eur. Ceram. Soc., 20 (4), p. 423; 
Lyckfeldt, O., Ferreira, J.M.F., (1998) J. Eur. Ceram. Soc., 18, p. 131; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., (2003) Advanced Materials Forum, , in press; 
Lyckfeldt, O., Brandt, J., Lesca, S., (2000) J. Eur. Ceram. Soc., 20 (14-15), p. 2551
},
	month = {2004///},
	pages = {1037 - 1040},
	address = {Porto},
	abstract = {Innovative methods for the production of porous bioceramics for implants with tailored pore volume fractions, pore sizes, pore size distributions and pore interconnectivity have been developed. The average pore sizes can vary from a few micrometers to more than one millimeter, therefore fulfilling all the needs required for components used in implantology. The methods combine in a suitable way the knowledge coming from different areas in the field of colloid and interface science, namely, on the processing of ceramic materials by traditional colloidal shaping techniques such as slip casting with a number of new direct consolidation techniques, which transform the fluid hydroxyapatite (HA) based suspensions into rigid bodies, and particularly the expertise in the production of porous ceramics by different techniques. Slip casting was preferably used for designing the cortical part of the bone, while direct consolidation techniques were used to "freeze" tailored foamy structures or suspensions containing different kinds of organic inclusions of suitable sizes and amounts that generate pores after burning out.},
	keywords = {Bioceramics, Biocompatibility, Bone, Cells, Ceramic materials, Consolidation techniques, hydroxyapatite, morphology, orthopedics, Pore size, Porous ceramics, Porous materials, Sintering, Slip casting},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516107\&partnerID=40\&md5=1bc01982f338c175b41fd08698d3da1c},
	author = {Lemos, A.F. and Ferreira, J.M.F.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2464,
	title = {Detection of Rib Borders on X-ray Chest Radiographs},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {3212},
	year = {2004},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: INEB - Instituto de Engenharia Biomedica, Laborat{\'o}ries de Sinai, Imagem Biom{\'e}dicaPortugalReferences: Volgelsang, F., Weiler, F., Dahmen, J., Kilbinger, M., Wein, B., G{\"u}nther, R.W., (1998) Detection and Compensation of Rib Structures in Chest Radiographs for Diagnose Assistance, Proceedings of SPIE, 3338, pp. 774-785;Park, M., Jin, J.S., Wilson, L.S., Detection and labeling ribs on expiration chest radiographs (2003) The Phisics of Medical Imaging Conference, SPIE, pp. 1021-1031. , 15-20 Feb; 
Ginneken, B., Romeny, Bart, M.H., Automatic delineation of ribs in frontal chest radiographs (2000) Medical Imaging 2000:Image Processing, Proceedings of SPIE, 3979, pp. 825-836; 
Monteiro, M., Mendon{\c c}a, A.M., Campilho, A., Lung field detection on chest radiographs (2002) Proceedings of VIIP02, pp. 367-372. , IASTED International Conference on Visualization, Imaging and Image Processing, Spain; 
Mendon{\c c}a, A.M., Suva, J., Campilho, A., Automatic delimitation of lung fields on chest radiographs (2004) Proceeding of ISBI04, IEEE International Symposium on Biomedical Imaging, , Arlington, USA; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Transactions on Systems, Man, and Cybernetics, 9 (1), pp. 62-66
},
	month = {2004///},
	pages = {108 - 115},
	abstract = {The purpose of the research herein presented is the automatic detection of the rib borders in posterior-anterior (PA) digital chest radiographs. In a computer-aided diagnosis system, the precise location of the ribs is important as it allows reducing the false positive in the detection of abnormalities such as nodules, rib lesions and lung lesions. We adopted an edge based approach aiming at detecting the lower border of each rib. For this purpose, the rib geometric model is described as a parabola. For each rib, the upper limit is obtained using the position of the corresponding lower border. {\textcopyright} Springer-Verlag 2004.},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-35048903160\&partnerID=40\&md5=8bc25ba1f1b611ae05e55a3597ccdef2},
	author = {Moreira, R. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2493,
	title = {Effect of the Solvents on the Solution Mixture Derived Polylactide/hydroxyapatite Composites},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Weng, W.; Dept. of Mat. Sci. and Engineering, Zhejiang University, Hangzhou 310027, China; email: wengwj@zju.edu.cnReferences: Hench, L.L., (1998) Journal of American Ceramic Society, 81, p. 1705;Wang, M., (2003) Biomaterials, 24, p. 2133; 
Hao, J., Yuan, M., Deng, X., (2001) Biomaterials, 22, p. 2867; 
Ignjatovic, N., Tomic, S., Dakic, M., Miljkovic, M., Plavsic, M., Uskokovic, D., (1999) Biomaterials, 20, p. 809; 
Misra, D.N., (1988) Langmuir, 4, p. 953; 
Dean, J.A., (1999) Lange{\textquoteright}s Handbook of Chemistry (15th Edition), , McGraw-HILL, INC. New York
},
	month = {2004///},
	pages = {477 - 480},
	address = {Porto},
	abstract = {Polylactide/hydroxyapatite composites were prepared by a solution mixture method. Chloroform and tetrahydrofuran were used as solvents. The effect of the solvents on the polylactide/hydroxyapatite composites was investigated by TEM, mechanical properties, sedimentation time and UV-Vis transmission spectra. The results indicate that the polylactide/hydroxyapatite prepared using tetrahydrofuran as solvent has better dispersion of the hydroxyapatite filler in the matrix and larger bend strength than the one using chloroform as solvent. This is ascribed to the proper interaction between the solvent and hydroxyapatite filler.},
	keywords = {Bend strength, Composite, Composite materials, dispersion, Dispersions, Fillers, Heat treatment, Hydroxvapatite, hydroxyapatite, Polarity, polylactide, Sedimentation, Solutions, Solvent, Solvents, transmission electron microscopy, Ultraviolet radiation},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346885571\&partnerID=40\&md5=4f0c563b563f23ea5102e4ef36cfd243},
	author = {Li, Y. and Weng, W. and Cheng, K. and Du, P. and Shen, G. and Han, G. and Lopes, M.A. and Santos, J.D.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2485,
	title = {Effects of some variables on R.F. Magnetron Sputtered Hydroxyapatite Coatings on Titanium},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Silva, R.A.; Inst. Sup. de Engenharia do Porto, Rua Dr. Antonio Bernardino A., 431, 4200-072 Porto, Portugal; email: ras@isep.ipp.ptReferences: Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., (1997) Journal of Materials Science: Materials in Medicine, 8, pp. 731-736;Raynaud, S., Champion, E., Bernache-Assolant, D., Tetard, D., (1998) Journal of Materials Science: Materials in Medicine, 9, pp. 221-227; 
Weng, J., Liu, X., Zhang, X., De Groot, K., (1996) Journal of Biomedical Materials Research, 30, pp. 5-11; 
Gross, K.A., Gross, V., Berndt, C.C., (1998) Journal of the American Ceramic Society, 81 (1), pp. 106-112; 
Edwards, J.T., Brunski, J.B., Higuchi, (1997) Journal of Biomedical Materials Research, 36, pp. 454-468; 
Yang, C.Y., Lin, R.M., Wang, B.C., Lee, T.M., Chang, E., Hang, Y.S., Chen, P.Q., (1997) Journal of Biomedical Materials Research, 37, pp. 335-345; 
Ogiso, M., Yamamura, M., Kuo, P.T., Borgese, D., Matsumoto, T., (1998) Journal of Biomedical Materials Research, 39, pp. 364-372; 
Van Dijk, K., Schaeken, H.G., Wolke, J.G.C., Maree, C.H.M., Habraken, F.H.P.M., Jansen, J.A., (1995) Materials in Clinical Applications, pp. 249-256. , P. Vincenzini, (ed.); 
Yoshinari, M., Hayakawa, T., Wolke, J.G.C., Nemoto, K., Jansen, J.A., (1997) Journal of Biomedical Materials Research, 37, pp. 60-67; 
Wolke, J.G.C., De Groot, K., Jansen, J.A., (1998) Journal of Biomedical Materials Research, 39, pp. 524-530
},
	month = {2004///},
	pages = {323 - 326},
	address = {Porto},
	abstract = {RF sputtering may be used to produce thin, well adherent bioactive coatings with long term stability to ensure contact with bone tissue. RF Sputtered Hydroxyapatite (HA) coatings were produced onto Ti at 200 and 150 W. S.E.M. analysis indicated that films obtained with 200 W were thicker than those with 150 W. Significant variations were observed, depending on the location of the substrates with respect to the sample holder in the sputtering chamber, with thickness ranging from 1.8 to 3.2 μm. EDS analysis of 200 W films revealed the presence of Ca and P, in ratios typical of HA. XPS of thinner films obtained at 150 W, showed Ca and P, but also some carbonate probably partially substituting OH. Anodic polarisation curves of samples coated at 200 W and 150 W and for different sputtering times, compared to Ti substrate, indicate that HA films were protective and that the degree of protection increased with film thickness, i.e., with power intensity and time of deposition.},
	keywords = {Bioactive coatings, Bone, Coatings, Corrosion protection, hydroxyapatite, Magnetron sputtering, Plasma spraying, Polarization, RF sputtering, Scanning electron microscopy, Sputter deposition, Sputtering chambers, Thin films, Titanium},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347659048\&partnerID=40\&md5=3b3caf6fcde1c71242d25a74b296f870},
	author = {Silva, R.A. and Sousa, M. and Monteiro, F.J. and Barral, R. and Santos, J.D. and Lopes, M.A.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2472,
	title = {Human serum albumin adsorption on TiO                    2 from single protein solutions and from plasma
},
	journal = {LangmuirLangmuir},
	volume = {20},
	year = {2004},
	note = {Cited By (since 1996): 58Export Date: 21 November 2012Source: ScopusCODEN: LANGDdoi: 10.1021/la049158dPubMed ID: 15491210Language of Original Document: EnglishCorrespondence Address: Sousa, S.R.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: ssousa@ibmc.up.ptChemicals/CAS: serum albumin, 9048-46-8; titanium dioxide, 1317-70-0, 1317-80-2, 13463-67-7, 51745-87-0; titanium, 7440-32-6; Serum Albumin; Solutions; titanium dioxide, 13463-67-7; Titanium, 7440-32-6References: Nakabayashi, N., Iwasaki, Y., (2000) European White Book on Fundamental Research in Materials Science, pp. pp70-71. , R{\"u}hle, M., Dosch, H., Mittemeijer, E. J.Van de Voorde, M. H., Eds.; Max Plank Institut fur Metallforschung: Stuttgart, Germany;Windler, M., Klabunde, R., (2001) Titanium in Medicine; Material Science, Surface Science, Engineering, Biological Responses and Medical Applications, pp. 703-746. , Brunette, D. M., Tengvall, P., Textor, M., Thomsen, P., Eds.; Springer-Verlag: Berlin, Germany; 
Larsson, C., Esposito, M., Liao, H., Thomsen, P., (2001) Titanium in Medicine: Material Science, Surface Science, Engineering, Biological Responses and Medical Applications, pp. 587-648. , Brunette, D.M., Tengvall, P., Textor, M., Thomsen, P., Eds.; Springer-Verlag: Berlin, Germany; 
Tengvall, P., Lundstrom, I., (1992) Clin. Mater., 9, pp. 116-134; 
Ostumi, E., Chapman, R.G., Liang, M.N., Meluleni, G., Pier, G., Ingberg, D.E., Whitesides, G.M., (2001) Langmuir, 17, pp. 6336-6343; 
Gehanno, V., Freitas, P.P., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Soares, J.C., Silva, M.F., (1999) IEEE Trans. Magn., 35, pp. 4361-4367; 
Owens, D.K., Wendt, E.C., (1969) J. Appl. Polym. Sci., 13, pp. 1741-1747; 
Kerkeb, M.L., Gonz{\'a}lez-Caballero, F., Chibowski, E., (1993) J. Colloid Interface Sci., 159, pp. 439-443; 
(1993) A Guide to Radioiodination Techniques; Amersham Life Science: Little Chalfont, p. 64. , Iodine-125, Buckinghamshire, U.K; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., (2001) J. Biomed. Mater. Res., 55, pp. 45-53; 
Williams, D.F., Askill, I.N., Smith, R., (1985) J. Biomed. Mater. Res., 19, pp. 313-320; 
Sheardown, H., Cornelius, R.M., Brash, J.L., (1997) Colloids Surf., B, 10, pp. 29-33; 
Du, Y.J., Cornelius, R.M., Brash, J.L., (2000) Colloids Surf., 17, pp. 59-67; 
Yu, X.J., Brash, J.L., (1993) Test Procedures for the Blood Compatibility of Biomaterials, pp. 287-330. , Dawids, S., Ed.; Kluwer Academic Publishers: Dordrecht, The Netherlands; 
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., (1985) Anal. Biochem., 150, pp. 76-85; 
Shirkhanzadeh, M., (1996) J. Mater. Sci.: Mater. Med., 6, pp. 206-210; 
Kilpadi, D.V., Raikar, G.N., Liu, J., Lemons, J.E., Vohra, Y., Gregory, J.C., (1998) J. Biomed. Mater. Res., 40, pp. 646-659; 
Lausmaa, J., Kasemo, B., Mattsson, H., (1990) Appl. Surf. Sci., 44, pp. 133-146; 
Armstrong, N.R., Quinn, R.K., (1977) Surf. Sci., 67, pp. 451-468; 
Leitao, E., Barbosa, M.A., Degroot, K., (1997) J. Mater. Sci.: Mater. Med., 8, pp. 423-426; 
Ramqvist, L., Hamrin, K., Johansso, G., Fahlman, A., Nordling, C., (1969) J. Phys. Chem. Solids, 30, pp. 1835-1847; 
Hanawa, T., Ota, M., (1992) Appl. Surf. Sci., 55, pp. 269-276; 
Kuznetsov, M.V., Zhuravlev, J.F., Gubanov, V.A., (1992) J. Electron Spectrosc. Relat. Phenom., 58, pp. 169-176; 
Ikeo, N., Iijima, Y., Niimura, N., Sigematsu, M., Tazava, T., Matsumoto, S., Kojima, K., Nagasawa, Y., (1991) Handbook of X-ray Photo-electron Spectroscopy JEOL, , Tokyo, Japan; 
Porte-Durrieu, M.C., Labrugere, C., Villars, F., Lefebvre, F., Dutoya, S., Guette, A., Bordenave, L., Baquey, C., (1999) J. Biomed. Mater. Res., 46, pp. 368-375; 
Wagner, C.D., Riggs, W.M., Davis, L.E., Moulder, J.F., (1979) Handbook of X-ray Photoelectron Spectroscopy, , Mulenberg, G. E., Ed.; Perkin Elmer Corp: Eden Prairie, MN; 
Barr, T.L., (1991) J. Vac. Sci. Technol., A, 9, pp. 1793-1805; 
W{\"a}livaara, B., Aronsson, B.O., Rodahl, M., Lausmaa, J., Tengvall, P., (1994) Biomaterials, 15, pp. 827-834; 
Oji, M.O., Wood, J.V., Downes, S., (1999) J. Mater. Sci.: Mater. Med., 10, pp. 869-872; 
Pan, J., Liao, H., Leygraf, C., Thierry, D., Li, J., (1998) J. Biomed. Mater. Res., 40, pp. 244-256; 
Ratner, B.D., Horbett, T.A., Shuttleworth, D., Thomas, H.R., (1981) J. Colloid Interface Sci., 83, pp. 630-642; 
Lindberg, B., Maripuu, R., Siegbahn, K., Larsson, R., Golander, C.G., Eriksson, J.C., (1983) J. Colloid Interface Sci., 95, pp. 308-321; 
Auditore, A., Satriano, C., Coscia, U., Ambrosone, G., Parisi, V., Marietta, G., (2002) Biomol. Eng., 19, pp. 85-90; 
Miller, B., Treppo, S., Voigt, A., Zingg, W., Neumann, A.W., (1993) Colloids Surf., 69, pp. 203-208; 
Serro, A.P.V., Fernandas, A.C., Saramago, B.D.V., (1997) Colloids Surf., A, 125, pp. 209-219; 
Lopes, M., Monteiro, F., Santos, J., Serro, A., Saramago, B., (1999) J. Biomed. Mater. Res., 45, pp. 370-375; 
Ferraz, M.P., Monteiro, F.J., Serro, A.P., Saramago, B., Gibson, I.R., Santos, J.D., (2001) Biomaterials, 22, pp. 3105-3112; 
Vogler, E.A., (1993) Surfactant Science Series, 49, pp. 183-250. , Wettability; Berg, J. C., Ed.; Marcel Dekker: New York; 
Feng, B., Weng, J., Yang, B.C., Qu, S.X., Zhang, X.D., (2003) Biomaterials, 24, pp. 4663-4670; 
Morra, M., Cassinelli, C., Cascardo, G., Cahalan, P., Cahalan, L., Fini, M., Giardino, R., (2003) Biomaterials, 24, pp. 4639-4654; 
W{\"a}livaara, B., Lundstrom, I., Tengvall, P., (1993) Clin. Mater., 12, pp. 141-148; 
Lincks, J., Boyan, B.D., Blanchard, C.R., Lohmann, C.H., Liu, Y., Cochran, D.L., Dean, D.D., Schwartz, Z., (1998) Biomaterials, 19, pp. 2219-2232; 
Ong, J.L., Carnes, D.L., Cardenas, H.L., Cavin, R., (1997) Implant Dent., 6, pp. 19-24; 
Ricci, J.L., Spivak, J.M., Blumenthal, N.C., (1991) The Bone Biomaterial Interface, pp. 334-349. , Davis, J. E., Ed.; University of Toronto Press: Toronto; 
Busscher, H.J., Vanpelt, A.W.J., Deboer, P., Dejong, H.P., Arends, (1984) J. Colloids Surf., 9, pp. 319-331; 
Hitchcock, S.J., Carroll, N.T., Nicholas, M.G., (1981) J. Mater. Sci., 16, pp. 714-732; 
Rakhorst, G., Van Der Mei, H.C., Van Oeveren, W., Spijker, H.T., Int, B.H.J., (1999) J. Artif. Organs, 22, pp. 35-39; 
Martins, M.C., Ratner, B.D., Barbosa, M.A., (2003) J. Biomed. Mater. Res., 67 A, pp. 158-171; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., (1998) J. Am. Chem. Soc., 120, pp. 3464-3473; 
Prime, K.L., Whitesides, G.M., (1991) Science, 252, pp. 1164-1167; 
Vandulm, P., Norde, W., (1983) J. Colloid Interface Sci., 91, pp. 248-255; 
Williams, R.L., Williams, D.F., (1988) J. Colloid Interface Sci., 126, pp. 596-603; 
Serro, A.P.V.A., Fernandes, A.C., Saramago, B.D.V., Norde, W., (1999) J. Biomed. Mater. Res., 46, pp. 376-381; 
Norde, W., Anusiem, A.C.I., (1992) Colloids Surf., 86, pp. 73-80; 
Young, B.R., Pitt, W.G., Cooper, S.L., (1988) J. Colloid Interface Sci., 125, pp. 246-260; 
Feng, L., Andrade, J.D., (1994) Biomaterials, 5, pp. 323-333; 
Norde, W., (1986) Adv. Colloid Interface Sci., 25, pp. 267-340; 
Kurrat, R., Prenosil, J.E., Ramsden, J.J., (1987) J. Colloid Interface Sci., 186, pp. 1-8; 
Brash, J.L., Uniyal, S., Samak, Q., (1974) Trans. Am. Soc. Artif. Intern. Organs, 20 A, pp. 69-76
},
	month = {2004///},
	pages = {9745 - 9754},
	abstract = {In the present work, the adsorption of human serum albumin (HSA) on commercially pure titanium with a titanium oxide layer formed in a H                        2O
                        2 solution (TiO
                        2 cp) and on TiO
                        2 sputtered on Si (TiO
                        2 sp) was analyzed. Adsorption isotherms, kinetic studies, and work of adhesion determinations were carried out. HSA exchangeability was also evaluated. Surface characterization was performed by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and wettability studies. The two TiO
                        2 surfaces have very distinct roughnesses, the TiO
                        2 sp having a mean R
                        a value 14 times smaller than the one of TiO
                        2 cp. XPS analysis revealed consistent peaks representative of TiO
                        2 on sputtered samples as well as on Ti cp substrate after 48 h of H
                        2O
                        2 immersion. Nitrogen was observed as soon as protein was present, while sulfur, present in disulfide bonds in HSA, was observed for concentrations of protein higher than 0.30 mg/mL. The work of adhesion was determined from contact angle measurements. As expected from the surface free energy values, the work of adhesion of HSA solution is higher for the TiO
                        2 cp substrate, the more hydrophilic one, and lower for the TiO
                        2 sp substrate, the more hydrophobic one. The work of adhesion between plasma and the substrates assumed even higher values for the TiO
                        2 cp surface, indicating a greater interaction between the surface and the complex protein solutions. Adsorption studies by radiolabeling of albumin (
                        125I-HSA) suggest that rapid HSA adsorption takes place on both surfaces, reaching a maximum value after ~60 min of incubation. For the higher HSA concentrations in solution, a multilayer coverage was observed on both substrates. After the adsorption step from single HSA solutions, the exchangeability of adsorbed HSA molecules by HSA in solution was evaluated. The HSA molecules adsorbed on TiO
                        2 sp seem to be more easily exchanged by HSA itself than those adsorbed on TiO
                        2 cp after 24 h. In contrast, after 72 h, nearly all the adsorbed albumin molecules effectively exchange with other albumin molecules.
},
	keywords = {Adsorption, article, Atomic force microscopy, Blood, chemistry, human, Human serum albumin (HSA), Humans, hydrophobicity, Multilayers, plasma, Plasmas, Plastic surgery, Proteins, Radiolabeling, Serum Albumin, solution and solubility, Solutions, Substrates, Surface characterization, Surface Properties, surface property, Titanium, Titanium dioxide, X ray photoelectron spectroscopy},
	isbn = {07437463 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-8344221996\&partnerID=40\&md5=60496aebf9155224cadbbb1456ff8a25},
	author = {Sousa, S.R. and Moradas-Ferreira, P. and Saramago, B. and Viseu Melo, L. and Barbosa, M.A.}
}
@conference {2470,
	title = {"In vitro" bioactivity evaluation of CaO-P                    2O 
                    5 glass-hydroxylapatite thin coatings produced by pulsed laser deposition
},
	booktitle = {Transactions - 7th World Biomaterials CongressTrans. World Biomater. Congr.},
	series = {Transactions - 7th World Biomaterials Congress},
	year = {2004},
	note = {Conference code: 64310Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Arias, J.L.; Depto. de F{\'\i}sica Aplicada, Universidade de Vigo, Lagoas-Marcosende 9, E-36200 Vigo, SpainReferences: Arias, J.L., (1997) J Mater Sci Mater Med, 8, p. 873;Ferraz, M.P., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., (1999) J. Mat Sci Mat Med, 10, p. 567
},
	month = {2004///},
	pages = {1774},
	address = {Sydney},
	abstract = {The synthesis of coatings based on hydroxyapatites (HA) and glass modified hydroxyapatite targets by pulsed laser deposition, was presented. It was observed that coatings produced from HA targets keep their crystallinity through all their thickness, while there is a loss of crystallinity towards the surface of the HA+1.5G coatings. On immersion in SBF for three days, the coatings produced from HA targets suffered a loss of crystallinity near the surfaces, while the crystallinity of the coatings produced from HA+1.5G targets increased. It was also observed that addition of 1.5wt\% of CaO-P                        2O
                        5 glasses to HA thin coatings produced by pulsed laser deposition leads to a significant structural change of the coating surface.
},
	keywords = {Apatites, Bioactivity, Calcium Compounds, Coatings, Crystalline materials, Crystalline phases, hydroxyapatite, Mixtures, Phosphorus Compounds, Pressure effects, Pulsed laser deposition, Scanning electron microscopy, Substrates, Thin coatings, X ray diffraction, X ray photoelectron spectroscopy},
	isbn = {1877040193 (ISBN); 9781877040191 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-13844299568\&partnerID=40\&md5=869dc43fe34d9345a4c3b944a06faad1},
	author = {Arias, J.L. and Liste, S. and Gonz{\'a}lez, P. and Serra, J. and Le{\'o}n, B. and Ferraz, M.P. and Monteiro, F.J. and Giao, D. and P{\'e}rez-Amor, M.}
}
@book {2489,
	title = {In Vitro Cytocompatibility of Osteoblastic Cells Cultured on Chitosan-Organosiloxane Hybrid Membrane},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Shirosaki, Y.; Biomaterial Laboratory, Faculty of Engineering, Okayama University, Tsushima, Okayama-shi 700-8530, JapanReferences: Chandy, T., Sharma, P.C., (1990) Biomat Art Cells Art Org, 18, pp. 1-24;Jameela, S.R., Jayakrishnan, A., (1995) Biomaterials, 16, pp. 769-775; 
Nishi, C., Nakajima, N., Ikada, Y., (1995) J Biomed Mater Res., 29, pp. 829-834; 
Ren, L., Tsuru, K., Hayajkawa, S., Osaka, A., (2002) Biomaterials, 23, pp. 4765-4773
},
	month = {2004///},
	pages = {857 - 860},
	address = {Porto},
	abstract = {Chitosan-silicate hybrids were synthesized using γ-glycidoxypropyltrimethoxysilane (GPSM) as the agent to cross-link the chitosan chains. Fourier-transform infrared (PT-IR) spectroscopy was used to analyze the structures of the hybrids. The swelling ability and the cytocompatibility of the hybrids were investigated as a function of the GPSM concentration. The swelling of the hybrids was suppressed by addition of GPSM. MG63 human osteoblastic cells were cultured on the hybrids. The adhesion and proliferation of the MG63 cells cultured on the chitosan-GPSM hybrid surface were improved comparing to that on chitosan membrane, regardless the GPSM concentration.},
	keywords = {Biocompatibility, Biological membranes, biosynthesis, Cell culture, Cells, chitosan, Chitosan-inorganic hybrid, Crosslinking, Cytocompatibility, Fourier transform infrared spectroscopy, Nuclear magnetic resonance spectroscopy, Organic polymers, Osteoblastic cell (MG63), Structural analysis, Synthetic polymers, Tissue, Tissue engineering, γ-Glycidoxypropyltrimethoxysilane (GPSM)},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346885502\&partnerID=40\&md5=80d45c86bb14c57c3b1a6b94b435e45d},
	author = {Shirosaki, Y. and Tsuru, K. and Hayakawa, S. and Osaka, A. and Lopes, M.A. and Santos, J.D. and Fernandes, M.H.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2499,
	title = {In Vitro Mineralisation of Human Bone Marrow Cells Cultured on Bonelike{\textregistered}},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Costa, M.A.; Inst. Cie. Blomed. de Abel Salazar, Universidade do Porto, Largo Abel Salazar, 4000 Porto, PortugalReferences: Santos, J.D., Campbell, J.K., Winston, G.H., European Patent N{\textdegree} WO0068164, 2000Lopes, M.A., Knowles, J.C., Santos, J.D., (2000) Biomaterials, 21, p. 1905;Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res.:App. Biomater., 48, p. 734; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (2000) Biomaterials, 21, p. 1165; 
Ferraz, M.P., Fernandes, M., Santos, J.D., Monteiro, F.J., (2001) J. Mat. Sci.: Mat. Med., 12, p. 629; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mater. Res., 54, p. 463; 
Duarte, F., Santos, J.D., Afonso, A., Advanced Materials Forum, , in press; 
Coelho, M.J., Fernandes, M.H., (2000) Biomaterials, 21, p. 1095; 
Kleerekoper, M., (1996) Principles of Bone Biology, p. 1053. , Academic Press
},
	month = {2004///},
	pages = {821 - 824},
	address = {Porto},
	abstract = {Bonelike{\textregistered} is a CaO-P                        2O
                        5 based glass-reinforced hydroxyapatite (HA) designed to mimic the inorganic composition of the bone tissue. This work evaluates the response of human bone marrow cells to Bonelike{\textregistered} concerning cell proliferation and osteoblast differentiation. HA was used as control material. Results showed that Bonelike{\textregistered} allowed the proliferation of bone marrow cells and their complete differentiation, as evidenced by the formation of cell-mediated mineralisation. In comparison with HA, Bonelike{\textregistered} had a positive effect on the expression of alkaline phosphatase and also on the formation of a mineralised matrix, two osteoblast markers.
},
	keywords = {Bone, Bonelike{\textregistered}, Cell culture, cell proliferation, Composition, Grafts, Human bone marrow cells, hydroxyapatite, In vitro mineralisation, mineralization, Scanning electron microscopy, Sintering, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346255201\&partnerID=40\&md5=470ac94fd75d1a53b887d44f59fbf7d1},
	author = {Costa, M.A. and Gutierres, M. and Almeida, L. and Lopes, M.A. and Santos, J.D. and Fernandes, M.H.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2487,
	title = {In Vivo Behaviour of Bonelike{\textregistered}/PLGA Hybrid: Histological Analysis and Peripheral Quantitative Computed Tomography (pQ-CT) Evaluation},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Oliveira, J.M.; Inst. de Engenharia Biom{\'e}dica, Lab. de Biomaterials, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jmoliv@fe.up.ptReferences: Solheim, E., Pinholt, E., Bang, G., (1992) Journal Neurosurgery, 76, pp. 275-279;Laurecin, C.T., Attawia, M.A., Lu, L.Q., Borden, M.D., Lu, H.H., Gorum, W.J., Lieberman, J.R., (2001) Biomaterials, 22, pp. 1271-1277; 
Nielsen, F.F., Karring, T., Gogolewski, S., (1992) Acta Orthop Scandinavica, 63, pp. 66-69; 
Jiang, G., Woo, B.H., Kang, F., Singh, J., Deluca, P.P., (2002) Journal of Controlled Release, 79, pp. 137-145; 
Duarte, F., Lopes, M.A., Santos, J.D., European Cells and Materials, , in press; 
Duarte, F., Afonso, A., Santos, J.D., Advanced Materials Forum, , in press; 
Oliveira, J.M., Lopes, M.A., Kawai, T., Ohtsuki, C., Santos, J.D., Afonso, A., Advanced Materials Forum, , in press; 
Santos, J.D., Campbell, J.K., Winton, G.H., European Patent N{\textdegree} W00068164 (2000)Oliveira, J.M., Miyazaki, T., Lopes, M.A., Ohtsuki, C., Santos, J.D., Biomaterials, , submitted
},
	month = {2004///},
	pages = {565 - 568},
	address = {Porto},
	abstract = {The present work consisted in assessing the in vivo biological behaviour of novel Bonelike{\textregistered}/PLGA hybrid materials through subcutaneous and tibiae implantations for a 4 week period using an animal model. No sign of inflammation was observed as a result of subcutaneous implantation, which reveals that these materials were well-tolerated by the soft tissues. pQ-CT analysis demonstrated that the implanted materials were highly osteointegrated in bone tissue. SEM analysis at bone defect area revealed that Bonelike{\textregistered} resorption occurred after 4 weeks of implantation. Although the osteoconductive properties of Bonelike{\textregistered} have been reported in literature, the present work allowed to demonstrate that the silane-coupling agent and the PLGA treatments do not significantly modify the biological performance of Bonelike{\textregistered}. This result is strongly encouraging since Bonelike{\textregistered}/PLGA hybrid materials are being developed for bone tissue healing/regeneration purposes with simultaneous delivery of therapeuthic molecules.},
	keywords = {Biodegradation, Bonelike{\textregistered}, Computerized tomography, Histological analysis, histology, hydroxyapatite, In vivo studies, Organic polymers, PLGA, pQ-CT, Silanes, Sintering, Synthetic polymers, Tibiae implantations, Tissue},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346255223\&partnerID=40\&md5=1d0c1ad508daa6b14d2d3aeabf5fff65},
	author = {Oliveira, J.M. and Kawai, T. and Lopes, M.A. and Ohtsuki, C. and Santos, J.D. and Afonso, A.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2475,
	title = {Laser surface modification of hydroxyapatite and glass-reinforced hydroxyapatite},
	journal = {BiomaterialsBiomaterials},
	volume = {25},
	year = {2004},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2003.11.054PubMed ID: 15120506Language of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB-Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: aqueiroz@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Durapatite, 1306-06-5; Macromolecular SubstancesReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical evaluation of porous and nonporous hydroxyapatite in the treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, pp. 1-10. , I.D.M. MacGregor. Oxford: Oxford University Press; 
Systemic antibiotics in periodontics (1996) J Periodontol, 67, pp. 831-838; 
Steinberg, D., Friedman, M., Soskolne, A., Sela, M.N., A new degradable controlled release device for treatment of periodontal disease: In vitro release study (1990) J Periodontol, 61, pp. 393-398; 
Soskolne, W.A., Heasman, H.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis: A multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Prado Da Silva, M.H., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., Porous glass reinforced hydroxyapatite materials produced with different organic additives (2002) J Non-Cryst Solids, 304, pp. 286-292; 
Barinov, S.M., Baschenko, Y.V., Application of ceramic composits as implants: Result and problem (1992) Bioceramics and the Human Body, pp. 206-210. , A. Krajewski. UK: Elsevier Science Publishers; 
Koshino, T., Murase, T., Takagi, T., Saito, T., New bone formation around porous hydroxyapatite wedge implanted in opening wedge high tibial osteotomy in patients with osteoarthritis (2001) Biomaterials, 22, pp. 1579-1582; 
Nishihara, K., Studies on peri-root tissue formation around new type artificial root made of dense hydroxyapatite (1993) Clin Mater, 12, pp. 159-167; 
Chang, B., Lee, C., Hong, K., Youn, H., Ryu, H., Chung, S., Park, K., Osteoconduction at porous hydroxyapatite with various pore configurations (2000) Biomaterials, 21, pp. 1291-1298; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., Reinforcement of hydroxyapatite by adding P 2O 5-CaO glasses with Na 2O, K 2O and MgO (1996) J Mater Sci: Mater Med, 7, pp. 187-189; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings (1999) J Biomed Mater Res, 47, pp. 603-611; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P2O5 Glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J Biomed Mater Res, 54, pp. 463-469; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., Adsorption and release of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites (2001) Biomaterials, 22, pp. 1393-1400; 
Cl{\`e}ries, L., Fern{\'a}ndez-Pradas, J.M., Morenza, J.L., Behaviour in simulated body fluid of calcium phosphate coating obtained by laser ablation (2000) Biomaterials, 21, pp. 1861-1865; 
D{\textquoteright}Alession, L., Teghil, R., Zaccardo, I., Ferro, D., Marotta, V., Pulsed laser ablation and deposition of bioactive glass as coating material for biomedical applications (1999) Appl Surf Sci, 138-139, pp. 527-532; 
Fern{\'a}ndez-Pradas, J.M., Cl{\`e}ries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the depositon rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys a, 72, pp. 613-618; 
Zeng, H., Lacefield, W.R., XPS, EDX and FTIR analysis of pulsed laser deposited calcium phosphate bioceramic coatings: The effects of various process parameters (2000) Biomaterials, 21, pp. 23-30; 
Serra, P., Fern{\'a}ndez-Pradas, J.M., Sardin, G., Morenza, J.L., Interaction effects of an excimer laser beam with hydroxyapatite targets (1997) Appl Surf Sci, 109-110, pp. 384-388; 
Serra, P., Morenza, J.L., Fluence dependence of hydroxyapatite laser ablantion plumes (1998) Thin Solid Films, 335, pp. 43-48; 
Foltyn, S.R., Dye, R.C., Ott, K.C., Peterson, E., Hubbard, K.M., Hutchinson, W., Muenchausen, R.E., Wu, X.D., Target modification in the excimer laser deposition of YBa 2Cu 3O 7-x thin films (1991) Appl Phys Lett, 59, pp. 594-596; 
Oliveira, V., Conde, O., Vilar, R., UV laser micromachining of ceramic materials: Formation of columnar topographies (2001) Adv Eng Mater, 3, pp. 75-81; 
Ricci, J.L., Alexander, H., Laser microtexturing of implant surfaces for enhanced tissue integration (2001) Key Eng Mater, 198-199, pp. 179-202; 
Khor, K.A., Vreeling, A., Dong, Z.L., Cheang, P., Laser treatment of plasma sprayed HA coatings (1999) Mater Sci Eng a, 266, pp. 1-7; 
Sobol, E.M., (1995) Phase transformations and ablation in laser-treated solids, , New York: Wiley; 
Underwood, E.E., (1970) Quantitative Stereology, , Reading: Addison-Wesley;; 
Underwood, E.E., Banerji, K., Quantitative fractography (1987) ASM-Metals Handbook, 12; 
Rey, C., Shimizu, M., Collins, B., Glimcher, M.J., Resolution enhanced Fourier transform infrared spectroscopy study of the environment of phosphate ion in the early deposits of a solid phase calcium phosphate in bone and enamel and their evolution with age: Investigations in the ν 4 PO 4 3- domain (1990) Calcif Tissue Int, 46, pp. 384-394; 
Rey, C., Shimizu, M., Collins, B., Glimcher, M.J., Resolution enhanced Fourier transform infrared spectroscopy study of the environment of phosphate ion in the early deposits of a solid phase calcium phosphate in bone and enamel and their evolution with age: Investigations in the ν 3 PO 4 3- domain (1991) Calcif Tissue Int, 49, pp. 383-388; 
Elliott, J.C., Structure and chemistry of apatites and other calcium orthophosphates (1994) Inorganic Chemistry, 18, p. 50. , Elliott J.C. Amsterdam: Elsevier Science; 
Dupraz, A., Nguyen, T.P., Richard, M., Dalcusi, G., Passuti, N., Influence of a cellulosic ether carrier on the structure of biphasic calcium phosphate ceramic particles in an injectable composite material (1999) Biomaterials, 20, pp. 663-673; 
Franke, R., Chass{\'e}, Th., Streubel, P., Meisel, A., Auger parameters and relaxation energies of phosphorus in solid compounds (1991) J Electron Spectrosc Relat Phemon, 56, pp. 381-388; 
Heitz, J., Pedarning, J.D., Bauerle, D., Petzow, G., Excimer laser ablation and micro-patterning of ceramic Si 3N 4 (1997) Appl Phys a, 65, pp. 259-261; 
Foltyn, S.R., Surface modification of materials by cumulative laser irradiation (1994) Pulsed Laser Deposition of Thin Films, pp. 89-113. , D.B. Chrisey, \& G.K. Hubler. New York: Wiley; 
Lappalainen, J., Frantti, J., Lanto, V., Surface structure and particulate generation in Pb 0.97Nd 0.02(Zr 0.55Ti 0.45)O 3 ablation targets during pulsed laser deposition (1999) J Am Cer Soc, 82, pp. 889-896; 
{\'O}brien, T.P., Lawler, J.F., Lunney, J.G., Blau, W.J., The effect of laser fluence on the ablation and deposition of YBa 2Cu 3O 7 (1992) Mater Sci Eng, 13, pp. 9-13; 
Man, H.C., Zhang, X.M., Yue, T.M., Lau, W.S., Excimer-laser surface modification of engineering ceramics for adhesive bonding (1997) J Mater Proc Techn, 66, pp. 123-129; 
S{\'a}nchez, F., Morenza, J.L., Trtik, V., Characterization of the progressive growth of columns by excimer laser irradiation of silicon (1999) Appl Phys Lett, 75, pp. 3303-3305; 
Pedraza, A., Fowlkes, J.D., Lowndes, D.H., Self organized silicon microcolumns arrays generated by pulsed laser irradiation (1999) Appl Phys a, 69, pp. 731-S734; 
Usoskin, A., Freyhardt, H.C., Krebs, H.U., Influence of light scattering on the development of laser induced ridge-cone structure on target surfaces (1999) Appl Phys a, 69, pp. 823-S826
},
	month = {2004///},
	pages = {4607 - 4614},
	abstract = {Surface treatment of materials with excimer laser radiation often results in the formation of a rough columnar or cone-shaped surface topography, which leads to a considerable increase in the surface area. As a result, the search for a non-porous bioactive material with adequate mechanical properties and a high surface to volume ratio, similar to porous materials, which could be used for drug delivery in the treatment of periodontitis, justified assessing excimer laser surface treatment to promote controlled roughning of hydroxyapatite (HA) and glass-reinforced hydroxyapatite (GR-HA). A KrF excimer laser with 248nm radiation wavelength and 30ns pulse duration was used for surface modification. The laser treatment was carried out in air, using wide ranges of radiation fluence and number of laser pulses. In order to identify the physico-chemical changes induced by the laser treatment and the column formation mechanisms in these materials, the treated surfaces were characterised by laser profilometry, scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infra-red spectroscopy (FTIR). Laser processing induced the formation of a surface topography consisting of cone-shaped features. The constitution of the surface layer was also modified, as revealed by FTIR, XPS and XRD. This work has shown that laser surface modification increases the surface area of HA and GR-HA and is a promising technique to increase the reactivity and drug delivery capability of both materials. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {article, Biocompatible Materials, Biomaterials, drug delivery system, Durapatite, Excimer laser, Excimer lasers, Fourier transform infrared spectroscopy, FTIR, glass, hydroxyapatite, infrared spectroscopy, laser, Lasers, Macromolecular Substances, materials testing, Molecular Conformation, Non-porous bioactive materials, periodontitis, physical chemistry, porosity, priority journal, Profilometry, Radiation Dosage, Reinforcement, Scanning electron microscopy, Surface area, Surface modification, Surface Properties, Surface treatment, topography, X ray diffraction, X ray diffraction analysis, X ray photoelectron spectroscopy, XPS, XRD},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2342427815\&partnerID=40\&md5=24da3c08c0c7b636d9d5039812944b52},
	author = {Queiroz, A.C. and Santos, J.D. and Vilar, R. and Eug{\'e}nio, S. and Monteiro, F.J.}
}
@book {2491,
	title = {Mineralised membranes for bone regeneration},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Mesquita, P.; Inst. de Engenharia Biom{\'e}dica, R. do Campo Alegre 823, 4150-180 Porto, PortugalReferences: Baron, M., Haas, R., D{\"o}rtbudak, O., Watzek, G., Experimentally induced peri-implantitis: A review (2000) Int J Oral Maxillofac Implants, 15, pp. 533-544;Hammerle, C.H., Jung, R.E., Feloutzis, A., A systematic review of the survival of implants in bone sites augmented with barrier membranes (guided bone regeneration) in partially edentulous patients (2002) J Clin. Periodontol., 29 (SUPPL. 3), pp. 226-231; 
Brunel, G., Brocard, D., Duffort, J.F., Jacquet, E., Justumus, P., Simonet, T., Benque, E., Bioabsorbable materials for guided bone regeneration prior to implant placement and 7-year followup: Report of 14 cases (2001) J Periodontol., 72 (2), pp. 257-264; 
Karapati, S., Hugson, A., Kugelberg, C.F., Healing following GTR treatment of bone defects distal to mandibular 2 nd molars after removal of impacted 3 rd molars (2000) J. Clin. Periodontol., 27, pp. 325-332; 
Sim{\~o}es, R., Fonseca, L., Vasconcelos, M., Afonso, A., Cavalheiro, J., Modified exoskeleton of shrimp for guided bone regeneration (2002) 17 th ESB, , Barcelona, Spain, September
},
	month = {2004///},
	pages = {1091 - 1094},
	address = {Porto},
	abstract = {Guided bone regeneration can be achieved using filling biomaterials and protective membranes. The aim of this work is to study a new membrane prepared from shrimp shells, without modification of the natural concave shape. The "in vitro" bioactivity was evaluated using a simulated body fluid prepared with substitution of calcium by strontium (SBR-Sr). The bioactivity behaviour was increased using a surface treatment with sodium silicate. The "in vitro" and "in vivo" tests allow to conclude that these membranes are bioactive and can induce bone growth on their surfaces.},
	keywords = {Biological membranes, biomaterial, Biomaterials, Bone, Bone Regeneration, Calcium, Ceramic materials, Deproteinization, Membrane, Mineralised membranes, Natural material, Optical microscopy, Shear strength, Sodium compounds, Strontium, Substitution reactions},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0348145956\&partnerID=40\&md5=51d6fa431f03c745e2def45321a0dfa0},
	author = {Mesquita, P. and Branco, R. and Afonso, A. and Vasconcelos, M. and Cavalheiro, J.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2466,
	title = {A model for educational simulation of infant cardiovascular physiology},
	journal = {Anesthesia and AnalgesiaAnesth. Analg.},
	volume = {99},
	year = {2004},
	note = {Cited By (since 1996): 19Export Date: 21 November 2012Source: ScopusCODEN: AACRAdoi: 10.1213/01.ANE.0000134797.52793.AFPubMed ID: 15562049Language of Original Document: EnglishCorrespondence Address: Goodwin, J.A.; Nemours Children{\textquoteright}s C., 807 Children{\textquoteright}s Way, Jacksonville, FL 32207, United States; email: jgoodwin@nemours.orgReferences: Good, M.L., Gravenstein, J.S., Training for safety in an anesthesia simulator (1993) Semin Anesth, 12, pp. 235-250;Gaba, D.M., DeAnda, A., The response of anesthesia trainees to simulated critical incidents (1989) Anesth Analg, 68, pp. 444-451; 
Howard, S.K., Gaba, D.M., Fish, K.J., Anesthesia crisis resource management training: Teaching anesthesiologists to handle critical incidents (1992) Aviat Space Environ Med, 63, pp. 763-770; 
Euliano, T.Y., Caton, D., Van Meurs, W., Good, M.L., Modeling obstetric cardiovascular physiology on a full-scale patient simulator (1997) J Clin Monit, 13, pp. 293-297; 
Halamek, L.P., Kaegi, D.M., Gaba, D.M., Time for a new paradigm in pediatric medical education: Teaching neonatal resuscitation in a simulated delivery room environment (2000) Pediatrics, 106, pp. E45; 
Beneken, J.E.W., (1965) A Mathematical Approach to Cardiovascular Function: The Uncontrolled Human System, , [PhD thesis]. Medisch Fysisch Institut TNO, Utrecht, The Netherlands; 
Beneken, J.E.W., DeWit, B., A physical approach to hemodynamic aspects of the human cardiovascular system (1967) Physical Bases of Circulatory Transport: Regulation and Exchange, pp. 1-45. , Reeve EB, Guyton AC, eds. Philadelphia: Saunders; 
Wesseling, K.H., Settels, J.J., Baromodulation explains short-term blood pressure variability (1985) Psychophysiology of Cardiovascular Control, pp. 69-97. , Orlebeke JF, Mulder G, van Doornen LJP, eds. New York: Plenum Press; 
Van Meurs, W.L., Good, M.L., Lampotang, S., Functional anatomy of full-scale patient simulators (1997) J Clin Monit, 13, pp. 317-324; 
Pruitt, A.W., Gersony, W.M., The cardiovascular system (1992) Nelson Textbook of Pediatrics. 14th Ed., pp. 1125-1227. , Behrman RE, ed. Philadelphia: Saunders; 
Gregory, G.A., Monitoring during surgery (1994) Pediatric Anesthesia, pp. 261-279. , Gregory GA, ed. New York: Churchill Livingstone; 
Graham Jr., T.P., Jarmakani, M.M., Evaluation of ventricular function in infants and children (1971) Pediatr Clin North Am, 18, pp. 1109-1132; 
Wodey, E., Plady, P., Copin, C., Comparative hemodynamic depression of sevoflurane versus halothane in infants (1997) Anesthesiology, 87, pp. 795-800; 
Suga, H., Sagawa, K., Shoukas, A.A., Load independence of the instantaneous pressure-volume ratio of the canine left ventricle and effects of epinephrine and heart rate on the ratio (1973) Circ Res, 2, pp. 314-322; 
Ten Voorde, B., (1992) Modeling the Baroreflex: A System Analysis Approach, , [PhD thesis]. Vakgroep Medische Fysica, Amsterdam, The Netherlands; 
Cunningham, M.D., Bedside pulmonary function testing of infants (1990) Essentials of Pediatric Intensive Care, pp. 878-883. , Levin DL, Morriss FC, eds. St. Louis: Quality Medical; 
Strafford, M.A., Cardiovascular physiology (1996) Smith{\textquoteright}s Anesthesia for Infants and Children, pp. 69-104. , Motoyama EK, Davis PJ, eds. St. Louis: Mosby; 
Lake, C.L., Cardiovascular anatomy and physiology (1992) Clinical Anesthesia, pp. 989-1020. , Barash PG, Cullen BF, Stoelting RK, eds. Philadelphia: Lippincott; 
Hsieh, K., Chen, P., Fu, S., A simple, noninvasive method to investigate vascular characteristics in children (1996) Angiology, 47, pp. 361-367; 
Stoelting, R.K., Miller, R.D., (2000) Basics of Anesthesia, pp. 364-375. , Philadelphia: Churchill Livingstone; 
Thilenius, O.G., Argilla, R.A., Angiographic right and left ventricular volume determination in normal infants and children (1974) Pediatr Res, 8, pp. 67-74; 
Drouin, E., Gournay, V., Calamel, J., Assessment of spontaneous baroreflex sensitivity in neonates (1997) Arch Dis Child Fetal Neonatal Ed, 76, pp. F108-12
},
	month = {2004///},
	pages = {1655 - 1664},
	abstract = {Full-body patient simulators provide the technology and the environment necessary for excellent clinical education while eliminating risk to the patient. The extension of simulator-based training into management of basic and critical situations in complex patient populations is natural. We describe the derivation of an infant cardiovascular model through the redefinition of a complete set of parameters for an existing adult model. Specifically, we document a stepwise parameter estimation process, explicit simplifying assumptions, and sources for these parameters. The simulated vital signs are within the target hemodynamic variables, and the simulated systemic arterial pressure wave form and left ventricular pressure volume loop are realistic. The system reacts appropriately to blood loss, and incorporation of aortic stenosis is straightforward. This infant cardiovascular model can form the basis for screen-based educational simulations. The model is also an essential step in attaining a full-body, model-driven infant simulator.},
	keywords = {aging, Algorithms, Anesthesiology, aorta stenosis, Aortic Valve Stenosis, arterial pressure, article, Baroreflex, biological model, bleeding, Blood Loss, Surgical, cardiovascular function, Cardiovascular physiology, Computer simulation, education, heart ventricle pressure, hemodynamic parameters, Hemodynamic Processes, Humans, Infant, Models, Biological, priority journal, Pulmonary Circulation, simulation, Ventricular Function, Left, Ventricular Function, Right},
	isbn = {00032999 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-9244231824\&partnerID=40\&md5=afb81c88d315abadfc3022fc3eba159a},
	author = {Goodwin, J.A. and van Meurs, W.L. and S{\'a} Couto, C.D. and Beneken, J.E.W. and Graves, S.A.}
}
@book {2492,
	title = {New Method for the Incorporation of Soluble Bioactive Glasses to Reinforce Porous HA Structures},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Lemos, A.F.; Dept. of Ceramic Engineering, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal; email: ilemos@cv.ua.ptReferences: De Groot, K., (1983) Bioceramics of Calcium Phosphates, , CRC Press, Boca Raton, FL;Wilson, L.L., Hench, J., (1993) An Introduction to Bioceramics, , World Scientific, UK; 
Hench, L.L., (1998) J. Am. Ceram. Soc., 81, p. 1075; 
Kohri, M., Miki, K., Waite, D.E., Nakajima, H., Okabe, T., (1993) Biomaterials, 14, p. 299; 
Krivak, N., Tas, A.C., (1998) J. Am. Ceram. Soc., 81, p. 2245; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., (2003) Advanced Materials Forum
},
	month = {2004///},
	pages = {1033 - 1036},
	address = {Porto},
	abstract = {The most widely used calcium-phosphate ceramic in clinical applications is hydroxyapatite, Ca                        10(PO
                        4)
                        6(OH)
                        2, however its dissolution rate in the human body after implantation is too low to achieve good results and it has poor mechanical properties. Recently, glass reinforced hydroxyapatite composites (GR-HA) have been developed, in order to improve the mechanical properties and to increase the similarity between implants and bone. The addition of a P
                        2O
                        5 based glass to a hydroxyapatite matrix, promotes the liquid phase sintering, improving the mechanical properties of the final bodies, and enabling the formation of two other calcium-phosphate phases, which have a higher dissolution rate, more compatible with bone regeneration. However, some of these glass compositions are soluble in water, making their incorporation in aqueous slurry impossible. Therefore, the aim of the present work is to study a new alternative method for the incorporation of soluble P
                        2O
                        5 based glass into macroporous structures obtained by different methods.
},
	keywords = {Bioactivation, Bioactive glass, Biocompatibility, Bone, Dissolution, glass, hydroxyapatite, Liquid phase sintering, Macroporous structures, Microstructure, Porous materials, Reinforced hydroxyapatite, Sintering, Slurries},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0348145989\&partnerID=40\&md5=18598c198e7e03dbf785151b7033c392},
	author = {Lemos, A.F. and Santos, J.D. and Ferreira, J.M.F.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2497,
	title = {Porous Hydroxyapatite and Glass Reinforced Hydroxyapatite for Controlled Release of Sodium Ampicillin},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., (1987) J Periodontol, 58, pp. 521-528;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, pp. 1-10. , MacGregor IDM, editor. Oxford: Oxford University Press; 
Steinberg, D., Friedman, M., Soskolne, A., Sela, M.N., (1990) J Periodontol, 61, pp. 393-398; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., (1996) J Mater Sci: Mater Med, 7, pp. 187-189; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J Biomed Mat Res, 45, pp. 376-383; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Key Engineering Materials, , submitted; 
Nishihara, K., (1993) Clin Materials, 12, pp. 159-167; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterials, 22, pp. 1393-1400
},
	month = {2004///},
	pages = {997 - 1000},
	address = {Porto},
	abstract = {Porous hydroxyapatite (HA) and glass reinforced hydroxyapatite (GRHA) with adequate macro and microporous structure were developed, aiming at being used as drug delivery carrier of antibiotics, for the in situ treatment of periodontitis. Materials were characterised by XRD and FTIR, presenting no changes from similar dense materials. Mercury intrusion porosimetry revealed micropores of less than 1 μm, accounting for 15\% of the total porosity. Compression tests have shown close values for HA and GRHA, with the former showing slightly higher values of strength. Ampicillin adsorption was more effective on porous than on dense HA, and was similar for HA and GRHA.},
	keywords = {Adsorption, Antibiotics, Coatings, Dense materials, Diseases, Drug delivery, Drug dosage, Fourier transform infrared spectroscopy, glass, Glass reinforced hydroxyapatite, hydroxyapatite, Mercury (metal), Porous, Porous materials, Scanning electron microscopy, Sodium, Strength of materials, Ultraviolet spectroscopy, X ray diffraction analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516111\&partnerID=40\&md5=a85b6393d2a4a2d3ee4b384bf4c3de78},
	author = {Queiroz, A.C. and Teixeira, S. and Santos, J.D. and Monteiro, F.J.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2490,
	title = {Preparation and Characterization of Calcium Phosphate Nanoparticles},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Manuel, C.M.; Inst. de Engenharia Biom{\'e}dica, Lab. de Biomaterials, Universidade do Porto, Rua do Campo Alegre, 823, 4150 -180 Porto, Portugal; email: cmmanuel@fe.up.ptReferences: Manuel, C.M., Ferraz, M.P., Monteiro, F.J., (2003) Key Engineering Materials, pp. 240-242;Jarcho, M., Bolen, C.H., Thomas, M.B., Bobick, J., Doremus, R.H., (1976) J Mat Sci, pp. 2027-2035; 
Jarcho, M., Salsbury, R.L., Thomas, M.B., Doremus, R.H., (1979) J Mat Sci, pp. 142-150; 
Puleo, D.A., Holleran, L.A., Doremus, R.H., Bizios, R., (1991) J Biomed Mat Res, 25, pp. 711-723; 
Fowler, B.O., Moreno, E.C., Brown, W.E., (1966) Arch. Oral Biol., 11, pp. 477-492
},
	month = {2004///},
	pages = {903 - 906},
	address = {Porto},
	abstract = {In this work, CaP nanoparticles were prepared by wet chemical precipitation using three different approaches and were characterised and quantified in terms of their composition. Different precipitating, ageing and drying temperatures were applied and affected HA to TCP ratio. Sintering induced formation of large agglomerates and affected the relative composition, specifically, increased concentrations of TCP as proved by SEM, FTIR and XRD. Different reactants also affected chemical final product composition. Contacts with fibroblasts and osteoblasts revealed better adhesion to sintered substrates.},
	keywords = {Agglomeration, Bioceramics, Biocompatibility, Bone, Calcium Compounds, Cell culture, Cytocompatibility, Fourier transform infrared spectroscopy, hydroxyapatite, nanoparticles, Nanostructured materials, orthopedics, Precipitation (chemical), Scanning electron microscopy, Synthesis (chemical), Tricalcium phosphate, Wet chemical precipitation, X ray diffraction analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346885495\&partnerID=40\&md5=a9f5b3807570c06984809bcb048ffc33},
	author = {Manuel, C.M. and Foster, M. and Monteiro, F.J. and Ferraz, M.P. and Doremus, R.H. and Bizios, R.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2477,
	title = {Production of porous hydroxyapatite with potential for controlled drug delivery},
	series = {Materials Science ForumMater Sci Forum},
	volume = {455-456},
	year = {2004},
	note = {Conference code: 63332Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: MSFOELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB, Inst. de Engenharia Biom{\'e}dica, R do Campo Alegre, 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., (1987) J. Periodontology, 58, p. 521;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, , Oxford University Press; 
(1996) Journal of Periodontology, 67, p. 831; 
Soskolne, W.A., Heasman, H.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., (1997) Journal of Periodontology, 68, p. 32; 
Da Silva, M.H.P., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., (2002) J. of Non-crystaline Solids, 304, p. 286; 
Chang, B., Lee, C., Hong, K., Youn, H., Ryu, H., Chung, S., Park, K., (2000) Biomat., 21, p. 1291; 
Ewers, R., Simons, B., (1992) Biomaterials - Hard Tissue Repair and Replacement, , Elsevier Sc; 
Ohgushi, H., Okumura, M., Yoshikawa, T., Inoue, K., Senpuku, N., Tamai, S., Shors, E.C., (1992) Journal of Biomedical Materials Research, 26, p. 885; 
Barinov, S.M., Baschenko, Y.V., (1992) Bioceramics and the Human Body, , Elsevier Sc; 
Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., (1997) J. Mater. Sc.: Materials in Medicine, 8, p. 731; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2003) Bioceramics, , accepted for; 
Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterial, 22, p. 1393
},
	month = {2004///},
	pages = {358 - 360},
	address = {Caparica},
	abstract = {Porous hydroxyapatite was prepared in order to be used as a drug carrier and promote bone ingrowth. For this purpose, the microstructure should have interconnective pores and micro as well as macro porosity, for bone cell adhesion and bone ingrowth. The porous hydroxyapatite prepared by the burn-out method exhibits all these features. For measuring porosity, SEM analysis, mercury intrusion porosimetry and BET were used. FT-IR and XRD analysis revealed that only hydroxyapatite phase was present, being this result similar to the one found for dense samples. The morphology obtained appears to be well adapted to bone growth and drug adsorption.},
	keywords = {Adsorption, Antibiotics, Bioceramics, Controlled drug delivery, Drug Carriers, Drug delivery, Fourier transform infrared spectroscopy, hydroxyapatite, morphology, Polyurethanes, porosity, Porous, Porous materials, Scanning electron microscopy, X ray diffraction analysis},
	isbn = {02555476 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17044442244\&partnerID=40\&md5=7baa6f22534360d57deb7862546fb275},
	author = {Queiroz, A.C. and Teixeira, S. and Santos, J.D. and Monteiro, F.J.},
	editor = {Martins R. and Fortunato E. and Ferreira I. and Dias C.}
}
@conference {2469,
	title = {Scheduling parallel tasks on heterogeneous clusters},
	booktitle = {Proceedings of the IASTED International Conference on Parallel and Distributed Computing and SystemsProc. IASTED INt. Conf. Parall. Distrib. Comput. Syst.},
	series = {Proceedings of the 16th IASTED International Conference on Parallel and Distributed Computing and Systems},
	volume = {16},
	year = {2004},
	note = {Conference code: 64182Export Date: 21 November 2012Source: ScopusArt. No.: 439-085Language of Original Document: EnglishCorrespondence Address: Barbosa, J.; Faculdade de Engenharia, Universidade do Porto, Inst. de Engenharia Biom{\'e}dica, Porto, Portugal; email: jbarbosa@fe.up.ptReferences: Amoura, A.K., Bampis, E., K{\"o}nig, J.-C., Scheduling algorithms for parallel gaussian elimination with communication costs (1998) IEEE Transactions on Parallel and Distributed Systems, 9 (7), pp. 679-686. , July;Barbosa, J., Padilha, A.J., Algorithm-dependent method to determine the optimal number of computers in parallel virtual machines (1998) VECPAR{\textquoteright}98, 1573. , Springer-Verlag LNCS; 
Barbosa, J., Tavares, J., Padilha, A., A group block distribution strategy for a heterogeneous ma chine (2002) Applied Informatics, pp. 378-383. , M. H. Hamza, editor. IASTED, ACTA Press, February; 
Barbosa, J., Tavares, J., Padilha, A.J., Linear algebra algorithms in a heterogeneous cluster of personal computers (2000) Proceedings of 9th Heterogeneous Computing Workshop, pp. 147-159. , IEEE CS Press, May; 
Berman, F., Wolski, R., Figueira, S., Schopf, J., Shao, G., Application-level scheduling on distributed heterogeneous networks (1996) Supercomputing, 96; 
Blazewicz, J., Dell{\textquoteright}Olmo, P., Drozdowski, M., Maczka, P., Scheduling multiprocessor tasks on parallel processors with limited availability (2003) European Journal of Operational Research, (149), pp. 377-389; 
Boudet, V., Rastello, F., Robert, Y., A proposal for a heterogeneous cluster ScaLAPACK(dense linear solvers) (1999) Proceedings of PDPTA, , CSREA Press; 
Boulet, P., Dongarra, J., Rastello, F., Robert, Y., Vivien, F., Algorithmic issues on heterogeneous computing platforms (1999) Parallel Processing Letters, 9 (2), pp. 197-213; 
Choi, J., Dongarra, J., Ostrouchov, L.S., Petitet, A.P., Walker, D.W., Whaley, R.C., The design and implementation of the ScaLAPACK LU, QR, and CHOLESKY factorization routines (1996) Scientific Programming, 5, pp. 173-184; 
Geijn, R., Watts, J., Summa: Scalable universal matrix multiplication algorithm (1995) Technical Report, CS-95-286. , University of Tennessee, Knoxville; 
Hopper, E., Turton, B.C.H., An empirical investigation of meta-heuristic and heuristic algorithms for a 2d packing problem (2001) European Journal of Operational Research, 128, pp. 34-57; 
Jansen, K., Scheduling malleable parallel tasks: An asymptotic fully polynomial time approximation scheme (2004) Algorithmica, 39, pp. 59-81; 
Lep{\`e}re, R., Mouni{\'e}, G., Trystram, D., An approximation algorithm for scheduling trees of malleable tasks (2002) European Journal of Operational Research, (142), pp. 242-249; 
Oh-Heum, Chwa, K.-Y., Scheduling parallel tasks with individual deadlines (1999) Theoretical Computer Science, 215, pp. 209-223; 
Shirazi, B., Wang, M., Pathak, G., Analysis and evaluation of heuristic methods for static task scheduling (1990) Journal of Parallel and Distributing Computing, 10, pp. 222-232; 
Topcuoglu, H., Hariri, S., Wu, M.-Y., Performance-effective and low-complexity task scheduling for heterogeneous computing (2002) IEEE Transactions on Parallel and Distributed Systems, 13 (3), pp. 260-274. , March; 
Trystram, D., Scheduling parallel applications using malleable tasks on clusters (2001) 15th International Conference on Parallel and Distributed Processing Symposium
Sponsors: Int. Association of Sci. and Technol. for Dev., IASTED; World Modelling and Simulation Forum (WMSF)},
	month = {2004///},
	pages = {449 - 454},
	address = {Cambridge, MA},
	abstract = {Parallel tasks, also called malleable tasks, are tasks that can be executed on any number of processors with its execution time being a function of the number of processors alloted to it. The scheduling of independent parallel tasks on homogeneous machines is a problem that has been extensively studied. For heterogeneous machines, with a diverse CPU capacity, new challenges arise, namely the selection of the set of CPUs that optimizes the processing time of a given task. This paper presents a methodology to determine the best allotment for each task that minimizes its processing time on a given heterogeneous machine. The aim is to improve the global processing time of a complex algorithm composed by several linear algebra kernels, by scheduling a number of parallel tasks in the heterogeneous machine. The results presented compare this approach to the simpler data parallel execution of each task on the heterogeneous cluster, i.e. executing one task at a time using the data parallel programming model.},
	keywords = {Computational complexity, Computer programming, CPU capacity, Data handling, Directed acyclic graphs (DAG), Graph theory, Heterogeneous clusters, Linear algebra, Parallel algorithms, Parallel processing systems, Parallel/malleable tasks, Program processors, Scheduling},
	isbn = {10272658 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-11844282098\&partnerID=40\&md5=c196fdfb0cc8b4c8a37688dd289071ca},
	author = {Barbosa, J. and Morais, C. and Monteiro, A.P.},
	editor = {Gonzalez T.}
}
@book {2496,
	title = {Study of a Silicate Cement for Dental Applications},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Pereira, S.; Fac. de Medicina Dent{\'a}ria, Universidade do Porto, R. Dr. Manuel Pereira da Silva, 4200-393 Porto, PortugalReferences: Craig, R.G., Rovers, J.M., (2002) Restorative Dental Materials. 9th Ed., pp. 593-627. , St Louis: Mosby;Gladwin, M., Bagby, M., (2000) Clinical Aspects of Dental Materials. First Edition, pp. 81-92. , Philadelphia: Lippincott Williams \& Wilkins; 
Leinfelder, K.F., Changing Restorative Traditions: The Use of Bases and Liners (1994) JADA, 125, pp. 65-67; 
Macchi, R.L., (2000) Mat. Dentales. 3 a Ed., pp. 129-135. , Buenos Aires: Edit. M{\'e}dica P.americana S.A; 
Wise, D.L., (1995) Encyclopedic Handbook of Biomaterials and Bioengineering, pp. 1398-1411. , New York: Marcel Dekver; 
Gordan, V.V., Mj{\"o}r, I.A., Hucke, R.D., Smith, G.E., Effect of Different Liner Treatments on Postoperative Sensivity of Amalgam Restorations (1999) Quintessence Int, 30, pp. 55-59; 
Me, M., Nakai, H., Effect of Immersion in Water on Linear Expansion and Strength of Three Base/Liner Materials (1995) Dent Mater, 14, pp. 70-77; 
Leevailoj, C., Cochran, M.A., Matis, B.A., Moore, B.K., Platt, J.A., Microleakage of Posterior Packable Resin Composites with and without Flowable Liners (2001) Oper Dent, 26, pp. 302-307; 
Weiner, R.S., Weiner, L.K., Kugel, G., Teaching the Use of Bases and Liners: A Survey of the North American Dental Schools (1996) JADA, 127, pp. 1640-1645; 
Pereira, S., Branco, R., Cavalheiro, J., Afonso, A., Vasconcelos, M., (2003) Sealing Ability of a New Dental Base Material, , Bioeng; 
(1991) Specification for Dental Water-based Cements, , ISO 9917 (F)
},
	month = {2004///},
	pages = {659 - 662},
	address = {Porto},
	abstract = {Dental cements have a wide field of applications in clinical practice. The use as a base material is a very important one in dentistry. The aim of this study is to assess some relevant properties of a silicate cement (SC) developed for dental applications, comparing it with two other cements already commercialised, a zinc phosphate cement De Trey Zinc Cement Improved{\textregistered} (DT) and ProRootMTA{\textregistered}(MTA). Their compressive strength, dimensional changes and weight variation in water after setting, according to ISO standards were compared. The mean compressive strength of DT, MTA and SC were, respectively, 134MPa, 31MPa and 46MPa. There were no statistically significant differences for the three tested cements at the various time intervals, in respect to their weight variation or dimensional changes. However, there was a little loss of weight for DT (0.15\%) and for MTA (3\%) and a gain of 3\% for the SC. Regarding the dimensional changes, all materials undergo little expansion, more significant in the MTA and in the SC samples of 2,07\% and 0,4\%, respectively. The new Silicate Cement, presented enough compressive strength, low weight variation, very small dimensional changes and good adaptation to the surface of dental cavity. For these reasons this new cement could be a valuable proposal as a base material for dental applications.},
	keywords = {Biocompatibility, Body fluids, Ceramic materials, ceramics, Compressive Strength, Dental cement, Dental cements, Dental prostheses, Dimensional stability, Precipitation (chemical), Scanning electron microscopy, Silicate cements, Silicates, Stainless steel, Weight variation},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346255102\&partnerID=40\&md5=b43c6052d3f4c9fcaff59b75a9cad543},
	author = {Pereira, S. and Cavalheiro, J. and Branco, R. and Afonso, A. and Vasconcelos, M.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2498,
	title = {The Valences of Egg White for Designing Smart Porous Bloceramics: As Foaming and Consolidation Agent},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Lemos, A.F.; Ceramic Engineering Department, Campus de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal; email: ilemos@cv.ua.ptReferences: De Groot, K., (1980) Biomaterials, 1, p. 47;Ohgushi, H., Okumura, M., Yoshikawa, T., Inoue, K., Senpuku, N., Tamai, S., Shors, E.C., (1992) J. Biomed. Mater. Res., 26, p. 885; 
Omatete, O., Janney, M.A., Strehlow, R.A., (1991) Am. Ceram Soc.Bull., 70, p. 1641; 
Kosmac, T., Novak, S., Sajko, M., (1995) Fourth Euro Ceramics Vol. 1, Basic Science: Developments, 1. , ed. C. Galassi. Gruppo Editoriale Faenza Editrice S.p.a., Italy; 
Graule, T.J., Baader, F.H., Gauckler, L.J., (1994) Cfi/Ber. DKH., 71, p. 317; 
Novich, B.E., Sundback, C.A., Adams, R.W., (1992) Ceramic Transactions, 26. , ed. M. J. Cima. American Ceramic Society, USA; 
Lycfeldt, O., Ferreira, J.M.F., (1998) J. Eur. Ceram. Soc., 18, p. 131; 
Rives, R.D., U. S. Patent 4113480 (1976)Lycfeldt, O., Brandt, J., Lesca, S., (2000) J. Eur. Ceram. Soc., 20 (14-15), p. 2551; 
Olhero, S.M., Tar{\`\i}, G., Coimbra, M.A., Ferreira, J.M.F., (2000) J. Europ. Ceram. Soc., 20 (4), p. 423; 
Dhara, S., Bhargava, P., (2001) J. Am. Ceram. Soc., 84 (12), p. 3048; 
Janney, M.A., Omatete, O., Walls, C., Nunn, S., Ogle, R., Westmoreland, G., (1998) J. Am. Ceram. Soc., 81 (3), p. 581; 
Lumry, R., Eyring, H., (1954) J. Phys. Chem., 58, p. 110; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., (2003) Advanced Materials Forum
},
	month = {2004///},
	pages = {1045 - 1048},
	address = {Porto},
	abstract = {Porous hydroxyapatite bioceramics were developed by a new direct consolidation method based on the foaming capability of egg white and on its gelling properties on heating, thus acting both as pore former and consolidator agent. The bubble forming capability of fresh egg white, due to the presence of a globular protein - albumen, allows the formation of a stable foam when added to a ceramic suspension. Adding a sufficient amount of the globular protein to the suspension revealed to be crucial to control either the pore volume fraction as well as the gelling behaviour and the rigidity of the green bodies. This consolidation method can be performed in nonporous moulds, enabling materials with controlled porosity to be obtained by varying the egg white content in the slip composition. After drying, burnout and sintering, materials with ultimate porosities close to 75 \% were obtained.},
	keywords = {Bioceramics, Biodegradation, Bone-grafting, Ceramic materials, Consolidation technique, Consolidation techniques, Egg-white, heating, hydroxyapatite, morphology, Pore size, porosity, Porous ceramics, Porous materials, Proteins, Slurries, Solidification},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516109\&partnerID=40\&md5=e6413ccdd076de29b881e380890e42ac},
	author = {Lemos, A.F. and Ferreira, J.M.F.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@conference {2468,
	title = {XRD and FT-IR characterisation of hydroxyapatite sputtered films on titanium},
	booktitle = {Transactions - 7th World Biomaterials CongressTrans. World Biomater. Congr.},
	series = {Transactions - 7th World Biomaterials Congress},
	year = {2004},
	note = {Conference code: 64310Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Silva, R.A.; Inst. Sup. de Engenharia do Porto, Rua Dr.Antonio Bernardino A., 431, 4200- 072 Porto, Portugal; email: ras@isep.ipp.ptReferences: Wolke, J.G.C., (1998) J.Biomed. Mat. Res, 39, pp. 524-530;Silva, R.A., Proceedings of Bioceramics 16, , to be published
},
	month = {2004///},
	pages = {163},
	address = {Sydney},
	abstract = {XRD and FT-IR were used to characterize hydroxyapatite (HA) films, which were prepared using rf sputtering. The morphology of the sample films was analyzed using SEM. From the FT-IR spectrum, sputtered HA films showed structural changes, namely the decrease of the strength of the liberational mode of OH group, indicating the presence of a poorly crystalline phase. XRD results of films obtained at 500 W and 400 W showed that the crystallinity of the 500 W 4h e 2h samples was low, just showing a very incipient degree of crystalline structural structural arrangement, while in the 400 W-4h and 2h samples there was no crystalline structure, with only a slight diffraction of the 002 and 301 HA planes.},
	keywords = {Absorption bands, Adsorption, Bioactive films, Biomaterials, Bone, crystal structure, Crystallinity, Deposition, Fourier transform infrared spectroscopy, hydroxyapatite, Hydroxyapatite films, morphology, Scanning electron microscopy, Sintering, Sputtering, Surface topography, Thin films, Titanium, X ray diffraction analysis},
	isbn = {1877040193 (ISBN); 9781877040191 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-13844280872\&partnerID=40\&md5=4b0c92bd00c80cb724aa79ae88c5976e},
	author = {Silva, R.A. and Sousa, M. and Monteiro, F.J. and Barrai, R. and Lopes, M.A.}
}
@article {2512,
	title = {Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(03)00244-8PubMed ID: 12818541Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB - Inst. de Engenharia Biomed., Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: gold, 7440-57-5; thiol derivative, 13940-21-1; water, 7732-18-5; Albumins; Alkanes; Biocompatible Materials; Gold, 7440-57-5; Serum Albumin; Sulfhydryl CompoundsReferences: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials science - an introduction to materials in medicine, , San Diego, CA, USA: Academic Press;Nakanishi, K., Sakiyama, T., Imamura, K., On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon (2001) J Biosci Bioeng, 91, pp. 233-244; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-78; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) J Am Chem Soc, 120, pp. 3464-3473; 
Taborelli, M., Eng, L., Descouts, P., Ranieri, J.P., Bellamkonda, R., Aebisher, P., Bovine serum albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopy (1995) J Biomed Mater Res, 29, pp. 707-714; 
Silin, V., Weetall, H., Vanderah, D., SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs) (1997) J Colloid Interface Sci, 185, pp. 94-103; 
Margel, S., Vogler, E.A., Firment, L., Watt, T., Haynie, S., Sogah, D.Y., Peptide, protein, and cellular interactions with self-assembled monolayer model surfaces (1993) J Biomed Mater Res, 27, pp. 1463-1476; 
Wadu-Mesthrige, K., Amro, N.A., Liu, G., Immobilization of proteins on self-assembled monolayers (2000) Scanning, 22, pp. 380-388; 
Takahara, A., Hara, Y., Kojio, K., Kajiyama, T., Plasma protein adsorption behaviour onto the surface of phase-separated organosilane monolayers on the basis of scanning force microscopy (2002) Colloids Surf B: Biointerfaces, 23, pp. 141-152; 
Kalltorp, M., Carl{\'e}n, A., Thomsen, P., Olsson, J., Tengvall, P., Analysis of rat plasma proteins desorbed from gold and methyl- and hydroxyl-terminated alkane thiols on gold surfaces (2000) J Mater Sci: Mater Med, 11, pp. 191-199; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Endothelial cell growth and protein adsorption on terminal functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Ulman, A., (1991) An introduction to ultrathin organic films: From Langmuir-Blodgett to self-assembled, , San Diego, CA, USA: Academic Press; 
Lin, J., Chuang, W., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionlity (2000) J Biomed Mater Res, 51, pp. 413-423; 
Mirsky, V.M., Riepl, M., Wolfbeis, O.S., Capacitive monitoring of protein immobilization and antigen/antibody reactions on monomolecular alkylthiol films on gold electrodes (1997) Biosensors Bioelectron, 12, pp. 977-989; 
Viana, A.S., Jones, A.H., Abrantes, L.M., Kalaji, M., Redox induced orientational changes in a series of short chain ferrocenyl alkyl thiols self-assembled on gold(1 1 1) electrodes (2001) J Electroanal Chem, 500, pp. 290-298; 
Kondo, T., Okamura, M., Uosaki, K., Anion effect on the electrochemical characteristics of a gold electrode modified with a self-assembled monolayer of ferrocenylhexanethiol in aqueous and dichlomethane solutions (2001) J Organometal Chem, 637-639, pp. 841-844; 
Dong, S., Li, J., Self-assembled monolayers of thiols on gold electrodes for bioelectrochemistry and biosensors (1997) Bioelectrochem Bioenergetics, 42, pp. 7-14; 
Gooding, J.J., Hibbert, D.B., The application of alkanethiol self-assembled monolayers to enzyme electrodes (1999) Trends Anal Chem, 18, pp. 525-533; 
Spangler, B.D., Tyler, B.J., Capture agents for quartz crystal microbalance-continuous flow biosensor: Functionalized self-assembled monolayers on gold (1999) Anal Chem Acta, 399, pp. 51-62; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Tengvall, P., Lundstrom, I., Liedberg, B., Protein adsorption studies on model organic surfaces: An ellipsometric and infrared spectroscopic approach (1998) Biomaterials, 19, pp. 407-422; 
Shen, D., Huang, M., Chow, L., Yang, M., Kinetic profile of the adsorption and conformational change of lysozyme on self-assembled monolayers as revealed by quartz crystal resonator (2001) Sensor Actuators B, 77, pp. 664-670; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, 2, pp. 183-214. , Williams D.F. Harcover: CRC Press; 
Du, Y.J., Cornelius, R.M., Brash, J.L., Measurement of protein adsorption to gold surface by radioiodination methods: Suppression of free iodide sorption (2000) Colloid Surf B, 17, pp. 59-67; 
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., Measurement of protein using bicinchoninic acid (1985) Anal Biochem, 150, pp. 756-785; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, p. 64. , Amersham Life Science. Little Chalfort, UK: Amersham International plc; 
Bain, C.D., Evall, J., Whitesides, G.M., Formation of monolayer coadsorption of thiols on gold: Variation in the head group, tail group and solvent (1989) J Am Chem Soc, 111, pp. 7155-7164; 
Castner, D.G., Hinds, K., Grainger, D.W., X-ray photoelectron spectroscopy sulfur 2p study of organic thiol and disulfide binding interactions with gold surfaces (1996) Langmuir, 12, pp. 5083-5086; 
Keller, H., Schrepp, W., Fuchs, H., Self-assembled organic films on gold and silver (1992) Thin Solid Films, 210-211, pp. 799-802; 
Chidsey, C.E.D., Loiacono, D.N., Chemical functionality in self-assembled monolayers: Structural and electrochemical properties (1990) Langmuir, 6, pp. 682-691; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy and electrochemistry (1987) J Am Chem Soc, 109, pp. 3559-3568; 
Miller, C., Cuendet, P., Gratzel, M., Adsorbed ω-hydroxy thiol monolayers on gold electrodes: Evidence for electron tunneling to redox species in solution (1991) J Phys Chem, 95, pp. 877-886; 
Krysinski, P., Brzostowska-Smolska, M., Capacitance characteristics of self-assembled monolayers on gold electrode (1998) Bioelectrochem Bioenergetics, 44, pp. 163-168; 
Guo, B., Anzai, J., OSA, T., Adsorption behaviour of serum albumin on electrodes surfaces and the effects of electrode potential (1996) Chem Pharm Bull, 44, pp. 800-803; 
Dent, A.H., Aslam, M., Other categories of protein coupling (1998) Bioconjugation, protein coupling techniques for the biomedical sciences, pp. 504-569. , M. Aslam, \& A.H. Dent. London: Macmillan; 
Seigal, R.R., Harder, P., Dahint, R., Grunze, M., On-line detection of nonspecific protein adsorption at artificial surfaces (1997) Anal Chem, 69, pp. 3321-3328; 
Norde, W., Giacomelli, C.E., BSA structural changes during homomolecular exchange between the adsorbed and the dissolved states (2000) J Biotechnol, 79, pp. 259-268; 
Peters T., Jr., (1996) All about albumin: Biochemistry, genetics, and medical applications, , San Diego, CA, USA: Academic Press; 
Feng, L., Andrade, J.D., Protein adsorption on low temperature isotropic carbon. III. Isoterms, competitivity, desorption and exchange of albumin and fibrinogen (1994) Biomaterials, 15, pp. 323-333
},
	month = {2003///},
	pages = {3697 - 3706},
	abstract = {Chronopotentiometry was used to study the adsorption of human serum albumin (HSA) to self-assembled monolayers with the following terminal functional groups: CH                        3, COOH and OH. Surfaces were characterized by X-ray photoelectron spectroscopy, water contact angle measurements and cyclic voltammetry. HSA coverage of the different SAMs was investigated by chronopotentiometry and the total amount of adsorbed protein was determined using radiolabelled albumin. Both techniques have demonstrated that HSA adsorption to the different SAM-modified electrodes increases in the following order: OH\&lt;COOH\&lt;CH
                        3-terminated SAMs. A good correlation between coverage and total amount of HSA adsorbed was observed for long adsorption times (900s). {\textcopyright} 2003 Elsevier Science Ltd. All rights reserved.
},
	keywords = {Adsorption, Albumin, Albumin adsorption, Albumins, alkane, Alkanes, article, Biocompatible Materials, biomaterial, Biomaterials, carboxyl group, Chemical modification, Chronopotentiometry, Contact angle, correlation analysis, cyclic potentiometry, Cyclic voltammetry, electrochemistry, electrode, Electron Probe Microanalysis, Functional groups, gold, Humans, hydroxyl group, isotope labeling, methyl group, Monolayers, potentiometry, priority journal, protein analysis, Self assembly, Self-assembled monolayers, Serum Albumin, Sulfhydryl Compounds, Surface modification, Surface Properties, surface property, temperature, thiol derivative, Time Factors, Water, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038802804\&partnerID=40\&md5=d9b75bc199d3c909e08c53f4de329ce5},
	author = {Martins, M.C.L. and Fonseca, C. and Barbosa, M.A. and Ratner, B.D.}
}
@article {2509,
	title = {Albumin adsorption on Cibacron Blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {14},
	year = {2003},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1026394431100Language of Original Document: EnglishCorrespondence Address: Martins, Ma.C.L.; INEB - Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: cibacron blue f3ga, 12236-82-7, 53127-08-5; ethylene glycol, 107-21-1; fibrinogen, 9001-32-5; gold, 7440-57-5; human serum albumin, 9048-49-1; iodine 125, 14158-31-7, 22822-81-7; macrogol, 25322-68-3; thiol derivative, 13940-21-1; water, 7732-18-5References: Ratner, B.D., (1996) J. Mol. Recognit., 9, p. 617;Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., (1998) Biomaterials, 10, p. 147; 
Dequeiroz, A.A.A., Barrak, E.R., Gil, H.A.C., Higa, O.Z., (1997) J. Biomater. Sci. Polym. Ed., 8, p. 667; 
Kamath, K.R., Park, K., (1994) J. Appl. Biomater., 5, p. 163; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., (1981) Trans. Am. Soc. Artif. Intern. Organs, 27, p. 499; 
Ji, J., Feng, L., Barbosa, M.A., (2001) Biomaterials, 22, p. 3015; 
Keogh, J.R., Eaton, J.W., (1994) J. Lab. Clin. Med., 121, p. 537; 
Wang, D., Chen, B., Ji, J., Feng, L., (2002) Bioconjugate Chem., 13, p. 792; 
Ji, J., Barbosa, M.A., Feng, L., Shen, J., (2002) J. Mat. Sci. Mat. Med., 13, p. 677; 
Mcfarland, C.D., Jenkins, M., Griesser, H.J., Chatelier, R.C., Steele, J.G., Underwood, P.A., (1998) J. Biomater. Sci. Polym. Ed., 9, p. 1207; 
Mcfarland, C.D., Filippis, C.D., Jenkins, M., Tunstell, A., Rhodes, N.P., Williams, D.F., Steele, J.G., (1998) J. Biomater. Sci. Polym. Ed., 9, p. 1277; 
Kopperschlager, G., Bohme, H.J., Hofmann, E., (1982) Adv. Biochem. Eng., 25, p. 101; 
Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannell, R., (1976) Biochem. J., 157, p. 301; 
Leatherbarrow, R.L., Dean, P.D.G., (1980) Biochem. J., 189, p. 27; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., (2003) J. Biomater. Sci. Polym. Ed., 14, p. 439; 
Dockal, M., Carter, D.C., Ruker, F., (1999) J. Biol. Chem., 274, p. 29303; 
Ostuni, E., Yan, L., Whitesides, G.M., (1999) Colloid Surf. B-Biointerfaces, 15, p. 3; 
Ulman, A., (1991) An Introduction to Ultrathin Organic Films: From Langmuir-Blodgett to Self-Assembled, , Academic Press Inc: San Diego, CA, USA; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., (1989) J. Am. Chem. Soc., 111, p. 321; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., (1987) J. Am. Chem. Soc., 109, p. 3559; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., (1998) J. Am. Chem. Soc., 120, p. 3464; 
Seigal, R.R., Harder, P., Dahint, R., Grunze, M., (1997) Anal. Chem., 69, p. 3321; 
L{\'o}pez, G.P., Biebuyck, H.A., Harter, R., Kumar, A., Whitesides, G.M., (1993) J. Am. Chem. Soc., 115, p. 10774; 
Prime, K., Whitesides, G.M., (1991) Science, 252, p. 1164; 
Chinn, J.A., Horbett, T.A., Ratner, B.D., (1991) Thromb. Haemost., 65, p. 608; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Silva, M.F., (1999) IEEE Trans. Magn., 35, p. 4361; 
Pale-Grosdemange, C., Simon, E.S., Prime, K.L., Whitesides, G.M., (1991) J. Am. Chem. Soc., 113, p. 12; 
Castner, D.G., Hinds, K., Grainger, D.W., (1996) Langmuir, 12, p. 5083; 
Lin, J.C., Chuang, W.H., (2000) J. Biomed. Mater. Res., 51, p. 413; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., (2001) J. Biomed. Mater. Res., 55, p. 45; 
(1993) Iodine-125, a Guide to Radioiodination Techniques - Amersham Life Science, p. 64. , Amersham International plc, Amersham Place, Little Chalfont, Bucks. HP7 9NA, England; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of Biocompatibility Tests, p. 183. , edited by D. F. Williams (Harcover, CRC Pr); 
Du, Y.J., Cornelius, R.M., Brash, J.L., (2000) Colloid Surf. B-Biointerfaces, 17, p. 59; 
Beamson, G., Briggs, D., (1992) High Resolution XPS of Organic Polymers - The Scienta ESCA300 Database, , John Wiley \& Sons Ltd, Chichester, England; 
htpp://www.lasurface.com/Tarlov, M.J., Burgess Jr., D.R.F., Gillen, G., (1993) J. Am. Chem. Soc., 115, p. 5305; 
Tegoulia, V.A., Cooper, S.L., (2000) J. Biom. Mater. Res., 50, p. 291; 
Ishida, T., Tsuneda, S., Nishida, N., Hara, M., Sasabe, H., Knoll, W., (1997) Langmuir, 13, p. 4638; 
Silver, J.H., Hergenrother, R.W., Lin, J.C., Lim, F., Lin, H.B., Okada, T., Chaudhury, M.K., Cooper, S.L., (1995) J. Biom. Mater. Res., 29, p. 535; 
Smith, T., (1980) J. Colloid. Interf. Sci., 75, p. 51; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., (1997) Langmuir, 13, p. 3404; 
Silin, V., Weetall, H., Vanderah, D., (1997) J. Colloid. Interf. Sci., 185, p. 94; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., (2003) J. Biom. Mater. Res., 67 A, p. 158; 
Schlereth, D.D., (1997) Sens. Actuator B-Chem., 43, p. 78; 
Mrksich, M., Grunwell, J.R., Whitesides, G.M., (1995) J. Am. Chem. Soc., 117, p. 12009; 
Hodneland, C.D., Lee, Y.S., Min, D.H., Mrksich, M., (2002) PNAS, 99, p. 5048; 
Malmsten, M., Lassen, B., (1994) J. Colloid. Interf. Sci., 166, p. 490; 
Feng, L., Andrade, J.D., (1994) Biomaterials, 15, p. 323; 
Bertilsson, L., Butt, H.J., Nelles, G., Schlereth, D.D., (1997) Biosens. Bioelectron., 12, p. 839
},
	month = {2003///},
	pages = {945 - 954},
	abstract = {Self-assembled monolayers can be tailored with specific ligands to a certain protein and at the same time prevent the non-specific adsorption of other proteins. Cibacron Blue F3G-A (CB-thiol) was successfully immobilized onto tetra(ethylene glycol)-terminated alkanethiol (CB-thiol). The affinity of human serum albumin (HSA) to immobilized Cibacron Blue F3G-A was studied using mixed thiolate self-assembled monolayers on gold with different n-alkyl chain lengths and functional terminal groups (CH                        3-; OH- and tetra(ethylene glycol)). Surfaces were characterized using X-ray photoelectron spectroscopy and water contact angle measurements. Albumin adsorption and exchangeability of the adsorbed albumin molecules with other albumin molecules in solution were evaluated using 
                        125I-radiolabeled HSA. Competitive adsorption between albumin and fibrinogen to the different self-assembled monolayers (SAMs) was also investigated. Results showed that the incorporation of CB-thiol on the monolayers does not increase the HSA adsorption and reversibility on the SAMs. However, although specific adsorption of HSA to the immobilized Cibacron Blue F3G-A was not demonstrated, the presence of CB-thiol decreases the affinity of fibrinogen to the OH-terminated SAMs. {\textcopyright} 2003 Kluwer Academic Publishers.
},
	keywords = {Adsorption, Albumin, aqueous solution, article, Cell immobilization, cibacron blue f3ga, Contact angle, controlled study, Drug products, Ethylene Glycol, fibrinogen, functional group, Glycols, gold, human, human serum albumin, iodine 125, isotope labeling, ligand, macrogol, molecule, priority journal, protein assembly, protein binding, protein immobilization, protein structure, Proteins, radioassay, Self assembly, surface property, synthesis, tetra(ethylene glycol) undecanethiol, thiol, thiol derivative, unclassified drug, Water, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0344629191\&partnerID=40\&md5=6ebb3020ea2ade3febc3906ce0672cc1},
	author = {Martins, Ma.C.L. and Naeemi, E. and Ratner, B.D. and Barbosa, M.A.}
}
@article {2515,
	title = {Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly (hydroxyethylmethacrylate)) surfaces},
	journal = {Journal of Biomaterials Science, Polymer EditionJ. Biomater. Sci. Polym. Ed.},
	volume = {14},
	year = {2003},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: JBSEEdoi: 10.1163/156856203766652057PubMed ID: 12807146Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: cibacron blue f3ga, 12236-82-7, 53127-08-5; dextran, 87915-38-6, 9014-78-2; fibrinogen, 9001-32-5; polymacon, 25053-81-0, 25249-16-5, 98932-78-6; polyurethan, 61789-63-7; Albumins; Biocompatible Materials; Cibacron Blue F 3GA, 12236-82-7; Fibrinogen, 9001-32-5; Membranes, Artificial; Polyhydroxyethyl Methacrylate, 25249-16-5; TriazinesReferences: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials Science - an Introduction to Materials in Medicine, , (Eds), Academic Press, San Diego, CA;Greco, R.S., (1994) Implantation Biology, the Host Response and Biomedical Devices, , (Ed.), CRC Press, Ann Arbour, MI; 
Sapatnekar, S., Anderson, J.M., (1999) Handbook of Biomaterials Evaluation. Scientific, Technical and Clinical Testing of Implant Materials, p. 353. , A. F. von Recum (Ed.), Taylor \& Francis, Philadelphia, PA; 
Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., (1998) Biomaterials, 10, p. 147; 
Keogh, J.R., Eaton, J.W., (1994) J. Lab. Clin. Med., 121, p. 537; 
Sapatnekar, S., Kieswetter, K.M., Merritt, K., Anderson, J.M., Cahalan, L., Verhoeven, M., Hendriks, M., Cahalan, P., (1995) J. Biomed. Mater Res., 29, p. 247; 
Paulsson, M., Kober, M., Freij-Larsson Stollenwerk, C., Wessl{\'e}n, B., Ljungh, {\r A}., (1993) Biomaterials, 14, p. 845; 
Kopperschlager, G., Bohme, H.J., Hofmann, E., (1982) Adv. Biochem. Eng., 25, p. 101; 
Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannell, R., (1976) Biochem. J., 157, p. 301; 
Arica, M.Y., Testereci, H.N., Denizli, A., (1998) J. Chromatogr A., 799, p. 83; 
Denizli, A., Salih, B., Senel, S., Arica, M.Y., (1998) J. Appl. Polym. Sci., 68, p. 657; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., (2003) Biomaterials, 24. , (in press); 
Leatherbarrow, R.L., Dean, P.D.G., (1980) Biochem. J., 189, p. 27; 
Horbett, T.A., (1986) Techniques of Biocompatibility Tests, 2, p. 183. , D. F. Williams (Ed.), CRC Press, Boca Raton, FL; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., (2001) J. Biomed. Mater. Res., 55, p. 45; 
(1993) Iodine-125, A Guide to Radioiodination Techniques, 64. , Amersham Life Science; 
Schrader, B., (1995) Infrared and Raman Spectroscopy, , (Ed.), VCH, Weinheim; 
Lamba, N.M.K., Woodhouse, K.A., Cooper, S.L., (1998) Polyurethanes in Biomedical Applications, , (Eds), CRC Press, Boca Raton, FL; 
http://www.lasurface.com/Beamson, G., Briggs, D., (1992) High Resolution XPS of Organic Polymers - The Scienta ESCA300 Database, , (Eds), Wiley, Chichester; 
Morra, M., Cassinelli, C., (1995) J. Biomed. Mater. Res., 29, p. 39; 
Compagnini, A., Fisichella, S., Foti, S., Maccarrone, G., Saletti, R., (1996) J. Chromatogr. A, 736, p. 115; 
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Kasori, Y., Shiroya, T., Shimura, K., Mochizuki, A., (2000) Biomaterials, 21, p. 1471; 
Feng, L., Andrade, J.D., (1994) Biomaterials, 15, p. 323; 
Peters T., Jr., (1996) All About Albumin. Biochemistry, Genetics and Medical Applications, , (Ed.), Academic Press, San Diego, CA; 
Dockal, M., Carter, D.C., R{\"u}ker, F., (1999) J. Biol. Chem., 274, p. 29303; 
Denizli, A., Kocakulak, M., Piskin, E., (1998) J. Chromatogr. B., 707, p. 25; 
Salih, B., Zenobi, R., (1998) Anal. Chem., 70, p. 1536; 
{\"O}sterberg, E., Bergstr{\"o}m, K., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., (1995) J. Biomed. Mater. Res., 29, p. 741; 
Beeskow, T., Kroner, K.H., Anspach, F.B., (1997) J. Colloid. Interf. Sci., 196, p. 278; 
Frazier, R.A., Matthijs, G., Davies, M.C., Roberts, C.J., Schacht, E., Tendler, S.J.B., (2000) Biomaterials, 21, p. 957; 
Bertilsson, L., Butt, H.J., Nelles, G., Schlereth, D.D., (1997) Biosens. Bioelectron., 12, p. 839; 
Kao, J.M., Rose, R., Yousef, M., Hunter, S.K., Rodgers, V.G.J., (1999) J. Biomed. Mater. Res., 47, p. 537; 
Tegoulia, V.A., Cooper, S.L., (2002) Colloid. Surf. B: Biointerf., 24, p. 217
},
	month = {2003///},
	pages = {439 - 455},
	abstract = {In the present work, it is intended to study the effect of Cibacron blue F3G-A (CB) immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces on protein adsorption and bacterial adhesion. CB immobilisation was carried out by covalent binding between its triazine ring and the hydroxyl groups of the polymer. Characterisation of the films was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR), contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). CB efficiency was evaluated using radiolabelled albumin and fibrinogen from pure solutions, mixtures of both and plasma. Bacterial adhesion tests before and after albumin pre-coating were also performed. The presence of CB increases albumin and fibrinogen adsorption to PU-PHEMA surfaces. The incorporation of CB onto the PU-PHEMA surface also increases bacterial adhesion. Although albumin pre-coating decreases bacterial adhesion onto PU (67\% decrease) and PU-PHEMA-CB (80\%), bacterial adhesion is always lower on PU and PU-PHEMA surfaces than on PU-PHEMA-CB. These results demonstrate that, in contrast to what has been described for CB bound to dextran, CB immobilisation on PU-PHEMA surfaces presents low selectivity to albumin and increased bacterial adhesion relatively to PU and PU-PHEMA surfaces.},
	keywords = {Adsorption, Albumin, Albumins, article, Bacterial adhesion, bacterium adherence, Biocompatible Materials, Chemical structure, Cibacron blue, cibacron blue f3ga, Contact angle, controlled study, covalent bond, dextran, fibrinogen, hydroxyl group, immobilization, infrared spectroscopy, isotope labeling, Membranes, Artificial, Microscopy, Electron, Scanning, nonhuman, PHEMA, Polyhydroxyethyl Methacrylate, polymacon, polyurethan, priority journal, Protein adsorption, Scanning electron microscopy, Spectroscopy, Fourier Transform Infrared, Staphylococcus epidermidis, Surface Properties, triazine derivative, Triazines, X ray photoelectron spectroscopy, XPS},
	isbn = {09205063 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038316702\&partnerID=40\&md5=6f911799b67ba3289c643eb874879e1f},
	author = {Martins, M.C.L. and Wang, D. and Ji, J. and Feng, L. and Barbosa, M.A.}
}
@article {2517,
	title = {Albumin and fibrinogen adsorption on PU-PHEMA surfaces},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 41Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(03)00002-4PubMed ID: 12628827Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; Laborat{\'o}rio de Biomateriais, Inst. de Engenharia Biom{\'e}dica, Rua do Campo Algre 823, Porto 4150-180, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: acrylic acid ethyl ester, 140-88-5; fibrinogen, 9001-32-5; poly(2 hydroxyethyl acrylate), 26022-14-0; polyetherurethan, 76600-67-4; polymacon, 25053-81-0, 25249-16-5, 98932-78-6; protein, 67254-75-5; Albumins; Biocompatible Materials; Fibrinogen, 9001-32-5; poly(2-hydroxyethyl acrylate), 26022-14-0; Polyhydroxyethyl Methacrylate, 25249-16-5; Water, 7732-18-5References: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials science - An introduction to materials in medicine, , CA, USA, San Diego: Academic Press;Greco, R.S., (1994) Implantation biology, the host response and biomedical devices, , Ann Arbour, USA: CRC Press; 
Sapatnekar, S., Anderson, J.M., Hemocompatibility: Effects on humoral elements (1999) Handbook of biomaterials evaluation, Scientific technical and clinical testing of implant materials, pp. 353-365. , A.F. von Recum. Philadelphia, PA, USA: Taylor \& Francis; 
Lopez, G.P., Ratner, B.D., Tidwell, C.D., Haycox, C.L., Rapoza, R.J., Horbett, T.A., Glow discharge plasma deposition of tetraethylene glycol dimethyl ether for fouling-resistant biomaterials surfaces (1992) J Biomed Mater Res, 26, pp. 415-439; 
Johnston, E.E., Bryers, J.D., Ratner, B.D., Interactions between Pseudomonas Aeruginosa and plasma-deposited PEO-like thin films during initial attachment and growth (1997) ACS Polym Prep, 38, pp. 1016-1017; 
Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., Effect of albumin coating on the in vitro blood compatibility of Dacron arterial prostheses (1998) Biomaterials, 10, pp. 147-155; 
Sapatnekar, S., Kieswetter, K.M., Merritt, K., Anderson, J.M., Cahalan, L., Verhoeven, M., Hendriks, M., Cahalan, P., Blood-biomaterial interactions in a flow system in the presence of bacterial, effect of protein adsorption (1995) J Biomed Mater Res, 29, pp. 247-256; 
Paulsson, M., Kober, M., Freij-Larsson, C., Stollenwerk Wessl{\'e}n, B., Ljungh, {\r A}., Adhesion of staphylococci to chemically modified and native polymers, and the influence of preadsorbed fibronectin, vitronectin and fibrinogen (1993) Biomaterials, 14, pp. 845-853; 
Keogh, J.R., Eaton, J.W., Albumin binding surfaces for biomaterials (1994) J Lab Clin Med, 121, pp. 537-545; 
Keogh, J.R., Velander, F.F., Eaton, J.W., Albumin-binding surfaces for implantable devices (1992) J Biomed Mater Res, 26, pp. 441-456; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., Alkyl substituted polymers with enhanced albumin affinity (1981) Trans Am Soc Artif Intern Organs, 27, pp. 499-503; 
Kamath, K.R., Park, K., Surface modification of polymeric biomaterials by albumin grafting using y-radiation (1994) J Appl Biomater, 5, pp. 163-173; 
McFarland, C.D., Filippis, C.D., Jenkins, M., Tunstell, A., Rhodes, N.P., Williams, D.F., Steele, J.G., Albumin binding surfaces: In vitro activity (1998) J Biomat Sci Polymer Ed, 9, pp. 1239-1277; 
Ji, J., Feng, L., Barbosa, M.A., Stearyl poly(ethylene oxide) grafted surfaces for preferential adsorption of albumin (2001) Biomaterials, 22, pp. 3015-3023; 
Tsai, C.C., Huo, H.H., Kulkarni, P., Eberhart, R.C., Biocompatible coatings with high albumin affinity (1990) ASAIO Trans, 36, pp. 307-M310; 
Bekos, E.J., Ranieri, J.P., Aebisher, P., Gardella J.A., Jr., Bright, F.V., Structural changes of bovine serum albumin upon adsorption to modified fluoropolymer substrates used for neural cell attachment studies (1995) Langmuir, 11, pp. 984-989; 
Bailly, A., Laurent, A., Lu, H., Elalami, I., Jacob, P., Mundler, O., Merland, J., Soria, C., Fibrinogen binding and platelet retention: Relationship with the thrombogenicity of catheters (1996) J Biomed Mater Res, 30, pp. 101-108; 
Montheard, J.P., Chatzopoulos, M., Chappard, D., 2-Hydroxyethyl methacrylate (HEMA): Chemical properties and applications in biomedical fields (1992) J M S-Rev Macromol Chem Phys, C32, pp. 1-34; 
Feng, X.D., Sun, Y.H., Qiu, K.Y., Reactive site and Mechanism of graft copolymerisation onto poly(ether urethane) with Ceric ion as initiator (1985) Macromolecules, 18, pp. 2105-2109; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, 2, pp. 183-214. , Williams DF, editor. Harcover: CRC Press; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, p. 64. , Amersham Life Science, Little Chalfont, England: Amersham International plc; 
Schrader, B., (1995) Infrared and Raman spectroscopy: methods and applications, , Weinheim: VCH Publishers, Inc., (Federal Republic of German); 
Lamba, N.M.K., Woodhouse, K.A., Cooper, S.L., (1998) Polyurethanes in biomedical applications, , Boca Raton, FL, USA: CRC Press; 
Bandekar, J., Sawyer, A., FT-IR spectroscopic studies of polyurethanes: IV studies of the effect of the presence of processing aids on the hemocompatibility of polyurethanes (1995) J Biomater Sci Polym Ed, 7, pp. 485-501; 
Tingey, K.G., Andrade, J.D., McGary C.W., Jr., Zdrahala, R.J., Surface analysis of commercial biomedical polyurethanes (1988) Polymer surface dynamics, , Andrade JD, editor. New York: Plenum Press; 
Beamson, G., Briggs, D., (1992) High resolution XPS of organic polymers - the scienta ESCA300 database, , Chichester, England: Wiley; 
Hsiue, G., Lee, S., Wang, C., Shiue, M.H., Chang, P.C., Plasma-induced graft copolymerisation of HEMA onto silicone rubber and TPX film improving rabbit corneal epithelial cell attachment and growth (1994) Biomaterials, 15, pp. 163-171; 
Lee, J.H., Ju, Y.M., Lee, W.K., Park, K.D., Kim, Y.H., Platelet adhesion onto segmented polyurethane surfaces modified by PEO - And sulfonated PEO-containing block copolymer additives (1998) J Biomed Mater Res, 40, pp. 314-323; 
Morra, M., Occhiello, E., Garbassi, F., On the wettability of poly(2-hydroxyethylmetacrylate) (1992) J Colloid Interface Sci, 149, pp. 84-91; 
Morra, M., Cassinelli, C., Surface field of forces and protein adsorption behavior of poly(hydroxyethylmethacrylate) films deposited from plasma (1995) J Biomed Mater Res, 29, pp. 39-45; 
Elam, J., Nygren, Adsorption of coagulation proteins from whole blood on to polymer materials: Relation to platelet activation (1992) Biomaterials, 13, pp. 3-8; 
Chin, J.A., Horbett, T.A., Ratner, B.D., Baboon fibrinogen adsorption and platelet adhesion to polymeric materials (1991) Thromb Haemostas, 65, pp. 608-617; 
Horbett, T.A., Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. II Compositional analysis with the prelabeled protein technique (1981) J Biom Mater Res, 15, pp. 673-695; 
Garrett, Q., Chatelier, R.C., Griesser, H.J., Milthorpe, B.K., Effect of charged groups on the adsorption and penetration of proteins onto and into carboxymethylated poly(HEMA) hydrogels (1998) Biomaterials, 19, pp. 2175-2186; 
Feng, L., Andrade, J.D., Protein adsorption on low temperature isotropic carbon. III. Isoterms, competitivity, desorption and exchange of albumin and fibrinogen (1994) Biomaterials, 15, pp. 323-333; 
Bohnert, J.L., Horbett, T.A., Changes in adsorbed fibrinogen and albumin interactions with polymers indicated by decreases in detergent elutability (1986) J Colloid Interface Sci, 111, pp. 363-377; 
Slack, S.M., Horbett, T.A., Changes in fibrinogen adsorbed to segmented polyurethanes and hydroxyethylmethacrylate-ethylmethacrylate copolymers (1992) J Biom Mater Res, 26, pp. 1633-1649; 
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Kasori, Y., Shiroya, T., Shimura, K., Mochizuki, A., Blood compatible aspects of poly(hydroxyethylacrylate) (PMEA) - Relationship between protein adsorption and platelet adhesion on PHEA surface (2000) Biomaterials, 21, pp. 1471-1481; 
Vroman, L., Adams, A.L., Fisher, G.C., Munoz, P.C., Interaction of high molecular weight kininogen, Factor XII and fibrinogen in plasma at interfaces (1980) Blood, 55, pp. 156-159; 
Vroman, L., Adams, A.L., Adsorption of proteins out of plasma and solutions in narrow spaces (1986) J Colloid Interface Sci, 111, pp. 391-402; 
Andrade, J.D., Hlady, V., Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses (1986) Adv Polym Sci, 79, pp. 1-63; 
Andrade, J.D., Hlady, V., Vroman effects, techniques and philosophies (1991) J Biomater Sci Polym Ed, 2, pp. 161-172; 
Brash, J.L., Studies of protein adsorption relevant to blood compatible materials (1991) Modern aspects of protein adsorption on biomaterials, p. 23. , Y.F. Missirlis, \& W. Lemm. Kluwer Netherland: Academic Publishers; 
Brash, J.L., Hove, P.T., Protein adsorption studies on standard polymeric materials (1993) J Biomater Sci Polymer Ed, 4, pp. 591-599
},
	month = {2003///},
	pages = {2067 - 2076},
	abstract = {Materials that adsorb specific proteins may find a variety of applications in the biomedical field. The aim of this study was the preparation of a hydrophilic surface, with low protein adsorption, to be used in the future as a support for the immobilisation of several species, e.g. Cibacron Blue F3G-A, which has been described to induce specific albumin adsorption. Poly(hydroxyethylmethacrylate) (PHEMA) and poly(hydroxyethylacrylate) (PHEA) were chosen as the hydrophilic surface because they can be easily polymerised and possess hydroxyl groups that can be used for the immobilisation of different compounds. Thin films of PHEMA and PHEA were successfully graft polymerised onto the surface of a commercial poly(etherurethane) (PU) using ceric ion as initiator. Grafting polymerisations were followed by mass gain and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Since stability tests demonstrated that only PU-PHEMA was stable in alkaline solutions, a necessary condition to future immobilisations, the investigation was focused on the coating of PU with PHEMA. PU-PHEMA films were characterised in detail using several techniques as mass gain, ATR-FTIR, contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Protein adsorption was evaluated using radiolabelled albumin and fibrinogen from pure solutions and from mixtures of both proteins. PU surfaces modified with PHEMA have demonstrated low protein adsorption, showing their potential use as substrates. This opens the possibly of exploring the advantages of selective adsorption by appropriate immobilisation of specific molecules. {\textcopyright} 2003 Elsevier Science Ltd. All rights reserved.},
	keywords = {acrylic acid ethyl ester, Adsorption, Albumin, Albumins, article, ATR-FTIR, Biocompatible Materials, Chemical modification, Dose-Response Relationship, Drug, fibrinogen, film, Fourier transform infrared spectroscopy, Graft polymerisation, Humans, Hydrogen-Ion Concentration, Hydrophilic surfaces, hydrophilicity, hydroxyl group, infrared spectroscopy, isotope labeling, Microscopy, Electron, Scanning, molecular stability, Pellethane, PHEMA, poly(2 hydroxyethyl acrylate), polyetherurethan, Polyhydroxyethyl Methacrylate, polymacon, Polymerization, priority journal, protein, Protein adsorption, Proteins, Scanning electron microscopy, Spectrometry, X-Ray Emission, Spectroscopy, Fourier Transform Infrared, surface property, Thin films, unclassified drug, Water, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037400503\&partnerID=40\&md5=dc989d7fe65e35d20f250cf3e7c245f9},
	author = {Martins, M.C.L. and Wang, D. and Ji, J. and Feng, L. and Barbosa, M.A.}
}
@conference {2503,
	title = {Detection and validation of lung field contours on chest radiographs},
	booktitle = {Proceedings of the IASTED International Conference on Biomedical EngineeringProc. IASTED Internat. Conf. Biomed. Eng.},
	series = {Proceedings of the IASTED International Conference on Biomedical Engineering},
	year = {2003},
	note = {Conference code: 63063Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Mendon{\c c}a, A.M.; INEB, Inst. de Engenharia Biom{\'e}dica, Campus da FEUP, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal; email: amendon@fe.up.ptReferences: Tsujii, O., Freedman, M., Mun, S., Automated segmentation of anatomic regions in chest radiographs using an adaptive-sized hybrid neural network (1998) Medical Physics, 25, pp. 998-1007;Armato III, S., Giger, M., MacMahon, H., Computerized detection of abnormal asymetry in digital lateral chest radiographs (1994) Medical Physics, 21, pp. 1761-1768; 
Armato III, S., Giger, M., Ashizawa, K., MacMahon, H., Automated lung segmentation in digital lateral chest radiographs (1998) Medical Physics, 25, pp. 1507-1520; 
Carrascal, F., Carreira, J., Souto, M., Automatic calculation of total lung capacity from automatically traced lung boundaries in posterior-anterior and lateral digital chest radiographs (1998) Medical Physics, 25, pp. 1118-1131; 
Ginneken, B., Romeny, B., Automatic segmentation of lung fields in chest radiographs (2000) Medical Physics, 27, pp. 2445-2455; 
Li, L., Zheng, Y., Kallergi, M., Clark, R., Improved method for automatic identification of lung regions on chest radiographs (2001) Academic Radiology, 8, pp. 629-638; 
Monteiro, M., Mendon{\c c}a, A.M., Campilho, A., Lung field detection on chest radiographs (2002) Proceedings of VIIP02, IASTED International Conference on Visualization, Imaging and Image Processing, pp. 367-372. , J. Villanueva (ed): Spain
Sponsors: IASTED},
	month = {2003///},
	pages = {183 - 188},
	address = {Salzburg},
	abstract = {The purpose of the research herein presented is the automatic detection of lung boundaries in posterior-anterior digital chest radiographs. The precise location of the two lungs is important in a computer-aided diagnosis system as it allows the reduction of the region under analysis, decreasing the computation time and facilitating data compression. Furthermore, it allows the delimitation of the search area, easing the selective tuning of the abnormalities detection algorithms. The results produced by the automatic method were validated by comparison with manual contours traced by an experienced radiologist. For this particular purpose, two programs with friendly interfaces were developed. The achieved comparison results demonstrate the good performance of the automatic method.},
	keywords = {Algorithms, automation, Biological organs, Chest radiographs, Computational time, Computer aided diagnosis, Computer-aided diagnosis, Data compression, Image analysis, Medical image processing, Medical imaging, Radiography, Regions of interest (ROI)},
	isbn = {0889863539 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2642575173\&partnerID=40\&md5=83abd9849c03da498665141b65efa36c},
	author = {Mendon{\c c}a, A.M. and Alves Da Silva, J. and Campilho, A.},
	editor = {Hamza M.H.}
}
@article {2507,
	title = {Development of foetal and neonatal simulators at the University of Porto},
	journal = {Medical Education, SupplementMed. Educ. Suppl.},
	volume = {37},
	year = {2003},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: MESECPubMed ID: 14641636Language of Original Document: EnglishCorrespondence Address: Van Meurs, W.L.; INEB - Inst. de Engenharia Biomedica, Lab. de Sinal e Imagem Biomedica, Edificio I-Poente, Rua Roberto Prias, s/n, 4200-465 Porto, Portugal; email: wvanmeurs@ineb.up.ptChemicals/CAS: oxygen, 7782-44-7References: Kohn, L.T., Corrigan, J.M., Donaldson, M.S., (2000) To Err Is Human, Building a Safer Health Care System, , Washington, DC: National Academic Press;Denson, J.S., Abrahamson, S., A computer-controlled patient simulator (1969) JAMA, 208, pp. 504-508; 
Gaba, D.M., DeAnda, A., A comprehensive anesthesia simulation environment. Re-creating the operating room for research and training (1988) Anesthesiology, 69, pp. 387-394; 
Good, M.L., Gravenstein, J.S., Anesthesia simulators and training devices (1989) Int Anesthesiol Clin, 27, pp. 161-168; 
Gaba, D., Howard, S., Fish, K., Smith, B., Sowb, Y., Simulation-based training in anesthesia crisis resource management (ACRM): A decade of experience (2001) Simulation Gaming, 32, pp. 175-193; 
Gordon, J.A., {\textquoteright}Practicing{\textquoteright} medicine without risk: Students{\textquoteright} and educators{\textquoteright} responses to high-fidelity patient simulation (2001) Acad Med, 76, pp. 469-472; 
Ma, O., Simulation saves lives (2001) Newsletter of the American Society of Anesthesiologists, , Park Ridge: American Society of Anesthesiologists; October; 
Van Meurs, W.L., Good, M.L., Lampotang, S., Functional anatomy of full-scale patient simulators (1997) J Clin Monit, 13, pp. 317-324; 
Van Meurs, W.L., Nikkelen, E., Good, M.L., Pharmacokinetic-pharmacodynamic model for educational simulations (1998) IEEE Trans Biomed Eng, 45, pp. 582-590; 
{\"O}hrn, M.A.K., Van Oostrom, J.H., Van Meurs, W.L., A comparison of traditional textbook and interactive computer learning of neuromuscular block (1997) Anesth Analg, 84, pp. 657-661; 
Euliano, T.Y., Caton, D., Van Meurs, W.L., Good, M.L., Modeling obstetric cardiovascular physiology on a full-scale patient simulator (1997) J Clin Monit, 13, pp. 293-297; 
S{\'a} Couto, P.M., Van Meurs, W.L., Bernardes, J.F., Marques De S{\'a}, J.P., Goodwin, J.A., Mathematical model for educational simulation of the oxygen delivery to the fetus (2002) Cont Eng Prac, 10, pp. 59-66; 
S{\'a} Couto, P.M., Van Meurs, W.L., Bernardes, J.F., Marques De S{\'a}, J.P., Goodwin, J.A., Combined model-driven and rule-based approach for educational simulation of fetal heart rate (2002) BIOSIGNAL 2002. Proceedings of the 16th Biennial International Conference of the European Association for Speech, Signal and Image Processing (EURASIP), 26-28 June 2002, Brno, Czech Republic, pp. 400-402. , Jan J, Kozumpl{\'\i}k J, Provazn{\'\i}k I, eds. Brno: Vutium Press; 
Itskovitz, J., LaGamma, E.F., Rudolph, A.M., Heart rate and blood pressure responses to umbilical cord compression in fetal lambs with special references to the mechanism of variable deceleration (1983) Am J Obstet Gynecol, 147, pp. 451-457; 
Lee, S.T., Hon, E.H., Fetal hemodynamic response to umbilical cord compression (1963) J Obstet Gynecol, 22, pp. 553-562; 
Goodlin, R.C., Lowe, E.W., A functional umbilical cord occlusion heart rate pattern - The significance of overshoot (1974) Obstet Gynecol, 43, pp. 22-30; 
Scheffs, J., Vasicka, A., Li, C., Solomon, N., Siler, W., Uterine blood flow during labor (1971) Obstet Gynecol, 38, pp. 15-24; 
Brotanek, V., Hendricks, C.H., Yoshida, T., Importance of changes in uterine blood flow in initiation of labor (1969) Am J Obstet Gynecol, 105, pp. 535-546; 
Butler, L.A., Longo, L.D., Power, G.G., Placental blood flows and oxygen transfer during uterine contractions: A mathematical model (1976) J Theor Biol, 61, pp. 81-95; 
Felgueiras, C., Marques De S{\'a}, J.P., Bernardes, J., Gama, S., Classification of foetal heart rate sequences based on fractal features (1998) Med Biol Eng Comput, 36, pp. 197-201; 
S{\'a} Couto, C.D., Van Meurs, W.L., Goodwin, J.A., Graphical and mathematical representation of congenital heart disease (2002) Proceedings of the SESAM Annual Meeting, 10-11 May 2002; Centro de Entrenamiento en Situaciones Cr{\'\i}ticas, Santander, Spain, p. 18; 
Beneken, J.E.W., (1965) A Mathematical Approach to Cardiovascular Function: The Uncontrolled Human System, , PhD dissertation. Utrecht, The Netherlands: Rijksuniversiteit Utrecht; 
Huikeshoven, F., Coleman, T.G., Jongsma, H.W., Mathematical model of the fetal cardiovascular system: The uncontrolled case (1980) Am J Physiol, 239, pp. R317-25; 
Klaus, M.H., Fanaroff, A.A., (2001) Care of the High-Risk Neonate, 5th Edn., , Philadelphia: WB Saunders
},
	month = {2003///},
	pages = {29 - 33},
	abstract = {Background: Human error can be expected to play a significant role in adverse outcomes in perinatal acute care. In acute care of the adult, simulator based training is recommended and used to improve patient safety. Aims: Our general goal is to develop model-driven foetal and neonatal educational simulators and curricula. The specific objectives of this paper are to introduce the reader to the perinatal acute care context and simulator design considerations, as well as to give initial results and describe ongoing developments. A brief description of adult simulators and simulation engines is followed by a more detailed description of a foetal distress simulator (FDS) and of models for a neonatal simulator developed at the University of Porto. Ongoing developments involve the modelling of foetal baroreflexes, the effect of uterine contractions, and an adapted method for estimating foetal heart rate variability parameters. Results: We present FDS simulation results reflecting the changes in oxygen supply to the foetus and the foetal heart rate in response to a reduction in uterine blood flow. We also present a structural diagram of a model for the educational simulation of congenital heart disease and preliminary simulation results reflecting a patent ductus arteriosus. Conclusion: We expect that, after initial clinical and educational validation, the presented models and simulators will play a role in simulator-based educational programmes contributing to increased perinatal safety.},
	keywords = {article, audiovisual equipment, blackstarPatient simulation, clinical competence, Clinical competence/blackstarstandards/economics, competence, Curriculum, Education, Medical, Undergraduate, Education, medical, undergraduate/blackstarmethods, Equipment Safety, fetus distress, human, Humans, Manikins, medical education, model, oxygen, Patient Simulation, perinatal care, Perinatal care/ blackstarstandards/methods, Portugal, safety, simulator, training},
	isbn = {13523929 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0242550943\&partnerID=40\&md5=4ad77934dd61ea164105b57b431c82a1},
	author = {van Meurs, W.L. and S{\'a} Couto, P.M. and S{\'a} Couto, C.D. and Bernardes, J.F. and Ayres-de-Campos, D.}
}
@article {2519,
	title = {Dissolution studies of hydroxyapatite and glass-reinforced hydroxyapatite ceramics},
	journal = {Materials CharacterizationMater Charact},
	volume = {50},
	year = {2003},
	note = {Conference code: 61615Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: MACHEdoi: 10.1016/S1044-5803(03)00092-5Language of Original Document: EnglishCorrespondence Address: Prado da Silva, M.H.; DEMP, UFC, Campus PICI, Bl. 714, Fortaleza, CE 60455-760, Brazil; email: mhprado@dem.ufc.brReferences: Barinov, S.M., Baschenko, Y.V., Application of ceramic composites as implants: Result and problem (1992) Bioceramics and the Human Body, pp. 206-210. , A. Ravaglioli, \& A. Krajewski. United Kingdom: Elsevier;Nishihara, K., Studies on peri-root tissue formation around new type artificial root made of dense hydroxyapatite (1993) Clin. Mater., 12, pp. 159-167; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J. Biomed. Mater. Res., 45, pp. 370-375; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., Reinforcement of hydroxyapatite by adding P 2O 5-CaO glasses with Na 2O, K 2O, MgO (1996) J. Mater. Sci. Mater. Med., 7, pp. 187-189; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings (1999) J. Biomed. Mater. Res., 47, pp. 603-611; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P 2O 5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J. Biomed. Mater. Res., 45, pp. 376-383; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J. Biomed. Mater. Res., 54, pp. 463-469; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J. Biomed. Mater. Res., 41, pp. 649-656; 
Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., Ca, P-rich layer formed on high-strength bioactive glass-ceramic A-W (1990) J. Biomed. Mater. Res., 24, pp. 331-343; 
Kokubo, T., Kushitani, H., Kitsugi, T., Yamamuro, T., Solution able to reproduce in vivo surface-structure changes of bioactive glass-ceramic A-W (1990) J. Biomed. Mater. Res., 24, pp. 721-734; 
Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., Features of calcium phosphate plasma-sprayed coatings: An in vitro study (1994) J. Biomed. Mater. Res., 28, pp. 961-967; 
Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., Calcium phosphate plasma-sprayed coatings and their stability: An in vivo study (1994) J. Biomed. Mater. Res., 28, pp. 909-917; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Yamamuro, T., Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid (1992) J. Mater. Sci. Mater. Med., 3, pp. 79-83; 
Zhang, H., Li, S., Yan, Y., Dissolution behaviour of hydroxyapatite powder in hydrothermal solution (2001) Ceram. Int., 27, pp. 451-454; 
LeGeros, R.Z., LeGeros, J.P., Calcium phosphate biomaterials in medical applications (1996) Bioceramics, 9, pp. 7-10. , T. Kokubo, T. Nakamura, \& F. Miyaji. Otsu, Japan: Elsevier; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., Adsorption and release studies of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites (2001) Biomaterials, 22, pp. 1393-1400; 
Langenbucher, F., Benz, D., K{\"u}rth, W., M{\"o}ller, H., Otz, M., Standardized flow-cell method as an alternative to existing pharmacopoeial dissolution testing (1989) Pharm. Ind., 51 (11), pp. 1276-1281; 
Kokubo, T., (1990) CRC Handbook of Bioactive Ceramics, 1, p. 41. , T. Yamamuro, L.L. Hench, \& J. Wilson. Boca Raton (FL): CRC Press; 
International Standard ISO/FDIS 10993-14, Biological evaluation of medical devices: Part 14 (2001) Identification and Quantification of Degradation Products from Ceramics
},
	month = {2003///},
	pages = {197 - 202},
	address = {Rio de Janeiro},
	abstract = {In the continuous agitation assays, glass-reinforced hydroxyapatite (GR-HA) was shown to form a calcium phosphate (CaP) layer, but hydroxyapatite (HA) only formed dispersed precipitates. The formation of this layer was first detected on the GR-HA with a 7.5\% glass addition (7.5 GR-HA) after only 3 days of immersion in simulated body fluid (SBF). The time required for layer formation decreased as the amount of glass added to the HA increased. The dissolution rate of the materials followed a similar pattern, i.e. the dissolution rate for GR-HA was higher than for HA, and increased with the addition of glass. The immersion of 7.5 GR-HA in water showed almost linear dissolution kinetics over the immersion periods (3, 7, 15, 30 and 60 days). The concentration of calcium ions in solution and the scanning electron microscopy (SEM) analysis of the 7.5 GR-HA specimens immersed in water and in SBF revealed a clear competition between the material dissolution and the precipitation of a CaP phase. Fourier transformed infrared spectroscopy with alternated total reflectance (FTIR-ATR) analysis indicated that the CaP phase that formed during longer immersion times (30 and 60 days) could be a carbonate-substituted CaP precipitate. As expected from previous work, the GR-HA behavior in terms of its in vitro bioactivity is higher than HA because a homogeneous CaP layer is formed and the precipitation occurs faster. From the dissolution test and in accordance with the chemical composition of the samples, GR-HA was more soluble than HA. {\textcopyright} 2003 Elsevier Inc. All rights reserved.},
	keywords = {Ceramic materials, Dissolution, glass, Glass-reinforced hydroxyapatite, hydroxyapatite, Immersion times, Scanning electron microscopy},
	isbn = {10445803 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0141895021\&partnerID=40\&md5=9226464f75e2ecd20d23f9d42e61d69e},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J. and Prado da Silva, M.H.},
	editor = {Solorzano G. and Freire Jr. F.L. and Soares G.A. and Souza P.L.}
}
@article {2510,
	title = {Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {67},
	year = {2003},
	note = {Cited By (since 1996): 63Export Date: 21 November 2012Source: ScopusCODEN: JBMRCPubMed ID: 14517873Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB-Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: human serum albumin, 9048-49-1; thiol derivative, 13940-21-1; Biocompatible Materials; Gold, 7440-57-5; Proteins; Sulfhydryl CompoundsReferences: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials Science - An Introduction to Materials in Medicine, , San Diego, CA: Academic;Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., Effect of albumin coating on the in vitro blood compatibility of Dacron arterial prostheses (1989) Biomaterials, 10, pp. 147-155; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., Alkyl substituted polymers with enhanced albumin affinity (1981) Trans Am Soc Artif Intern Organs, 27, pp. 499-503; 
Kamath, K.R., Park, K., Surface modification of polymeric biomaterials by albumin grafting using γγρ;-radiation (1994) J Appl Biomater, 5, pp. 163-173; 
McFarland, C.D., Filippis, C.D., Jenkins, M., Tunstell, A., Rhodes, N.P., Williams, D.F., Steele, J.G., Albumin binding surfaces: In vitro activitv (1998) J Biomater Sci Polym Ed, 9, pp. 1277-1239; 
Ji, J., Feng, L., Barbosa, M.A., Stearyl poly(ethylene oxide) grafted surfaces for preferential adsorption of albumin (2001) Biomaterials, 22, pp. 3015-3023; 
McFarland, C.D., Jenkins, M., Griesser, H.J., Chatelier, R.C., Steele, J.G., Underwood, P.A., Albumin binding surfaces: Synthesis and characterization (1998) J Biomater Sci Polym Ed, 9, pp. 1207-1225; 
Keogh, J.R., Eaton, J.W., Albumin binding surfaces for biomaterials (1994) J Lab Clin Med, 121, pp. 537-545; 
Tsai, C.C., Huo, H.H., Kulkarni, P., Eberhart, R.C., Biocompatible coatings with high albumin affinity (1990) ASAIO Trans, 36, pp. M307-M310; 
Bekos, E.J., Ranieri, J.P., Aebisher, P., Gardella Jr., J.A., Bright, F.V., Structural changes of bovine serum albumin upon adsorption to modified fluoropolymer substrates used for neural cell attachment studies (1995) Langmuir, 11, pp. 984-989; 
Bailly, A., Laurent, A., Lu, H., Elalami, I., Jacob, P., Mundler, O., Merland, J., Soria, C., Fibrinogen binding and platelet retention: Relationship with the thrombogenicity of catheters (1996) J Biomed Mater Res, 30, pp. 101-108; 
Margel, S., Vogler, E.A., Firment, L., Watt, T., Haynie, S., Sogah, D.Y., Peptide, protein, and cellular interactions with self-assembled monolayer model surfaces (1993) J Biomed Mater Res, 27, pp. 1463-1476; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-78; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Endothelial cell growth and protein adsorption on terminal functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Ulman, A., (1991) An Introduction to Ultrathin Organic Films: From Langmuir-blodgett to Self-assembled, , San Diego, CA: Academic; 
Lestelius, M., (1996) Tailor-made Monolayer Assemblies for in Vitro Studies of Blood Protein-surface Interactions, , PhD Thesis. Link{\"o}ping University, Sweden; 
Kalltorp, M., Carl{\'e}n, A., Thomsen, P., Olsson, J., Tengvall, P., Analysis of rat plasma proteins desorbed from gold and methyl- and hydroxyl-terminated alkane thiols on gold surfaces (2000) J Mater Sci Mater Med, 11, pp. 191-199; 
Tengvall, P., Lundstrom, I., Liedberg, B., Protein adsorption studies on model organic surfaces: An ellipsometric and infrared spectroscopic approach (1998) Biomaterials, 19, pp. 407-422; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) J Am Chem Soc, 120, pp. 3464-3473; 
L{\'o}pez, G.P., Biebuyck, H.A., Harter, R., Kumar, A., Whitesides, G.M., Fabrication and imaging of two-dimensional patterns of proteins adsorbed on self-assembled monolayers by scanning electron microscopy (1993) J Am Chem Soc, 115, pp. 10774-10781; 
Silin, V., Weetall, H., Vanderah, D., SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs) (1997) J Colloid Interface Sci, 185, pp. 94-103; 
Bain, C.D., Whitesides, G.M., Formation of monolayers by the coadsorption of thiols on gold: Variation in the length of the alkyl chain (1989) J Am Chem Soc, 111, pp. 7164-7175; 
Bain, C.D., Evall, J., Whitesides, G.M., Formation of monolayer coadsorption of thiols on gold: Variation in the head group, tail group and solvent (1989) J Am Chem Soc, 111, pp. 7155-7164; 
Bain, C.D., Whitesides, G.M., Correlations between wettability and structure in monolayers of alkanethiols adsorbed on gold (1988) J Am Chem Soc, 110, pp. 3665-3666; 
Folkers, J.P., Laibirds, P.E., Whitesides, G.M., Self-assembled monolayers of alkanethiols on gold: Comparisons of monolayers containing mixtures of short- and long-chain constituents with CH 3 and CH 2OH terminal groups (1992) Langmuir, 8, pp. 1330-1341; 
Atre, S.V., Liedberg, B., Allara, D.L., Chain length dependence of the structure and wetting properties in binary composition monolayers of OH- and CH 3-terminated alkanethiolates on gold (1995) Langmuir, 11, pp. 3882-3893; 
Pan, S., Belu, A.M., Ratner, B.D., Self assembly of 16-mercapto-1-hexandecanol on gold: Surface characterization and kinetics (1997) Mat Sci Eng, C7, pp. 51-58; 
Adamson, A.W., Gast, A.P., (1997) Physical Chemistry of Surfaces, , New York: Wiley; 
Vogler, E.A., (1993) Interfacial Chemistry in Biomaterials Science. Surfactant Science, Series 49, pp. 183-250. , Beng JC, editor. New York: Marcel Dekker; 
Miller, R., Treppo, S., Voight, A., Zingg, W., Neumann, A.W., Contact angle kinetics of human albumin solutions at solid surfaces (1993) Colloids Surf, 69, pp. 203-208; 
Van Der Vegt, W., Van Der Mei, H.C., Busscher, H.J., Interfacial free energies in protein solution droplets on FEP-teflon by axisymmetric drop shape analysis by profile-IgG versus BSA (1993) J Colloid Interface Sci, 156, pp. 129-136; 
Serro, A.P.V., Fernandes, A.C., Saramago, B.J.V., Dynamic interfacial behaviour of bovine serum albumin solutions on titanium surfaces (1997) Colloid Surf A, 125, pp. 209-219; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of Biocompatibility Tests, 2, pp. 183-214. , Williams DF, editor. Boca Raton, FL: CRC Press; 
Du, Y.J., Cornelius, R.M., Brash, J.L., Measurement of protein adsorption to gold surface by radioiodination methods: Suppression of free iodide sorption (2000) Colloid Surf B, 17, pp. 59-67; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Feng, L., Andrade, J.D., Protein adsorption on low temperature isotropic carbon. III. Isoterms, competitivity, desorption and exchange of human albumin and fibrinogen (1994) Biomaterials, 15, pp. 323-333; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, p. 64. , Little Chalfont, England: Amersham Life Sciences, Amersham International, PLC; 
Ishida, T., Tsuneda, S., Nishida, N., Hara, M., Sasabe, H., Knoll, W., Surface-conditioning effect of gold substrates on octadecanethiol self-assembled monolayer growth (1997) Langmuir, 13, pp. 4638-4643; 
Smith, T., The hydrophilic nature of a clean gold surface (1980) J Colloid Interface Sci, 75, pp. 51-55; 
Castner, D.G., Hinds, K., Grainger, D.W., X-ray photoelectron spectroscopy sulfur 2p study of organic thiol and disulfide binding interactions with gold surfaces (1996) Langmuir, 12, pp. 5083-5086; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy and electrochemistry (1987) J Am Chem Soc, 109, pp. 3559-3568; 
Nuzzo, R.G., Dubois, L.H., Allara, D.L., Fundamental studies of microscopic wetting on organic surfaces. Formation and structural characterization of a self consistent series of polyfunctional organic monolayers (1990) J Am Chem Soc, 112, pp. 558-569; 
Chidsey, C.E.D., Loiacono, D.N., Chemical functionality in self-assembled monolayers: Structural and electrochemical properties (1990) Langmuir, 6, pp. 682-691; 
Roeges, N.P.G., (1994) A Guide to the Complete Interpretation of Infrared Spectra of Organic Structures, , Chichester, England: John Wiley \& Sons, Inc; 
Van Dulm, P., Norde, W., The adsorption of human plasma albumin on solid surfaces, with special attention to the kinetic aspects (1983) J Colloid Interface Sci, 91, pp. 248-255; 
Lee, J.H., Kim, H.W., Pak, P.K., Kim, S.S., Lee, H.B., Protein behaviour on polymer surfaces with hydrophilic functional groups (1994) Korea Polym J, 2, pp. 32-39; 
Peters, T., Serum albumin (1985) Adv Protein Chem, 37, pp. 161-240; 
Prime, K.L., Whitesides, G.M., Self-assembled organic monolayers: Model systems for studying adsorption of proteins at surfaces (1991) Science, 252, pp. 1164-1167; 
Malmsten, M., Lassen, B., Competitive adsorption at hydrophobic surfaces from binary protein systems (1994) J Colloid Interface Sci, 166, pp. 490-498; 
Petrash, S., Cregger, T., Zhao, B., Pokidysheva, E., Foster, M.D., Brittain, W.J., Sevastianov, V., Majkrzak, C.F., Changes in protein adsorption on self-assembled monolayers with monolayer order: Comparison of human serum albumin and human gamma globulin (2001) Langmuir-on Line, A, pp. G; 
Kurrat, R., Prenosil, J.E., Ramsden, J.J., Kinetics of human and bovine serum albumin adsorption at silica-titania surfaces (1997) J Colloid Interface Sci, 185, pp. 1-8; 
Andrade, J.D., Hlady, V., Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses (1986) Advances Polymer Science, , Dusek K, editor. Heidelberg: Springer-Verlag
},
	month = {2003///},
	pages = {158 - 171},
	abstract = {The effect of surface composition and wettability on the adsorption of human serum albumin (HSA) was studied. Self-assembled monolayers (SAMs) containing mixtures of longer chain methyl- and shorter chain hydroxyl-terminated alkanethiols on gold were used to produce a range of surfaces with different wettabilities and exposed functional groups. Different SAMs were characterized by X-ray photoelectron spectroscopy, water contact angles, and Fourier transform infrared reflection absorption spectroscopy (IRAS). HSA adsorption onto the different SAMs was evaluated by contact angle measurements (wetting tension determinations), radiolabeling of proteins, and IRAS. Concerning HSA adsorption, all the techniques demonstrated higher HSA adsorption on more hydrophobic surfaces. The wetting tension measurements and IRAS suggested a gradual decrease of the HSA adsorption with increases of surface hydrophilicity. Radiolabeled albumin measurements also demonstrated a significant decrease of HSA adsorption on the pure hydroxyl-terminated SAMs. However, no significant differences were detected between mixed and pure methyl-terminated SAMs. Studies of HSA exchangeability with human fibrinogen have suggested that an ideal percentage of hydroxyl groups on the surface may increase albumin affinity without fibrinogen adsorption. {\textcopyright} 2003 Wiley Periodicals, Inc.},
	keywords = {Absorption spectroscopy, Adsorption, article, Biocompatible Materials, Contact angle, Contact angles, fourier transform infrared reflection absorption spectroscopy, Fourier transform infrared spectroscopy, Functional groups, gold, human serum albumin, hydrophilicity, hydrophobicity, IRAS, isotope labeling, measurement, Mixtures, monolayer culture, Monolayers, Protein adsorption, Proteins, Self-assembled monolayers, Spectrum Analysis, Sulfhydryl Compounds, surface property, tension, thiol derivative, Time Factors, wettability, X ray photoelectron spectroscopy, XPS},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0344306504\&partnerID=40\&md5=cc1823cda1406a9f9572da09315251f7},
	author = {Martins, M.C.L. and Ratner, B.D. and Barbosa, M.A.}
}
@book {2518,
	title = {Synthesis of hydroxyapatite and tricalcium phosphate nanoparticles - Preliminary studies},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {240-242},
	year = {2003},
	note = {Conference code: 60907Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Manuel, C.M.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: candida@ibmc.up.ptReferences: Rhee, S.-H., (2002) Biomaterials, 23, pp. 1147-1152;Yeong, K.C.B., Wang, J., Ng, S.C., (2001) Biomaterials, 22, pp. 2705-2712; 
Fowler, B.O., Moreno, E.C., Brown, W.E., (1966) Arch. Oral Biol., 11, pp. 477-492
},
	month = {2003///},
	pages = {555 - 558},
	address = {Sydney, NSW},
	abstract = {The wet mechanochemical process (MC) and chemical precipitation (CP) used to prepare hydroxyapatite (HA) and tricalcium phosphate (TCP) nanoparticles proved to be successful. In mechanochemical treatment, water and ethanol were used as a solvent but the last one improved particles agglomeration and decreased HA formation. This effect was also observed when aging temperature was decreased in wet chemical precipitation.},
	keywords = {Agglomeration, Calcium Compounds, Chemical precipitation, hydroxyapatite, Mechanochemical treatment, nanoparticles, Nanostructured materials, Precipitation (chemical), Synthesis (chemical), Tricalcium phosphate},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037246970\&partnerID=40\&md5=a4396f71d0ffef576b372e3227b9bb41},
	author = {Manuel, C.M. and Ferraz, M.P. and Monteiro, F.J.},
	editor = {Ben - Nissan B. and Sher D. and Walsh W.}
}
@article {2534,
	title = {Mathematical model for educational simulation of the oxygen delivery to the fetus},
	journal = {Control Engineering PracticeControl Eng. Pract.},
	volume = {10},
	year = {2002},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: COEPEdoi: 10.1016/S0967-0661(01)00133-2Language of Original Document: EnglishCorrespondence Address: Marques de S{\'a}, J.P.; Instituto de Engenharia Biomedica, Faculdade de Engenharia, Universidade do Porto, Rua Roberto Frias, Porto 4200-465, Portugal; email: jmsa@fe.up.ptReferences: Beneken, J.E.W., Rideout, V.C., The use of multiple models in cardiovascular system studies: Transport and perturbation methods (1968) IEEE Transactions on Bio-Medical Engineering, 15 (4), pp. 281-289;Cunningham, G., (1997) Williams Obstretrics, , (20th ed.). London: Prentice-Hall; 
Delivoria-Papadopoulos, M., McGowan, J.E., Oxygen transport and delivery (1998) Fetal and Neonatal Physiology, pp. 1105-1117. , R. A. Polin and W. W. Fox (Eds.), (2nd ed.) London: W.B. Saunders Company; 
Euliano, T.Y., Caton, D., van Meurs, W.L., Good, M.L., Modeling obstetric cardiovascular physiology on a full-scale patient simulator (1997) Journal Clinical Monitoring, 13 (5), pp. 293-297; 
Fukui, Y., Smith, N.T., Interactions among ventilation, the circulation, and the uptake and distribution of halothane-use of a hybrid computer multiple model: I the basic model (1981) Anesthesiology, 54, pp. 107-118; 
Fukui, Y., Smith, N.T., Interactions among ventilation, the circulation, and the uptake and distribution of halothane-use of a hybrid computer multiple model: II spontaneous vs. controlled ventilation, and the effects of CO 2 (1981) Anesthesiology, 54, pp. 119-124; 
Gaba, D.M., De Anda, A., A comprehensive anesthesia simulation environment: Recreating the operating room for research and training (1988) Anesthesiology, 69, pp. 387-394; 
Good, M.L., Gravenstein, J.S., Anesthesia simulators and training devices (1989) International Anesthesiololy Clinics, 27 (3), pp. 161-168; 
Guyton, A.C., (1991) Medical Physiolology, , (8th ed.). London: W.B. Saunders Company; 
Henson, L., Lee, A., (1998) Simulators in Anesthesiology Education, , New York: Plenum; 
Huikeshoven, F.J., Hope, I.D., Power, G.G., Gilbert, R.D., Longo, L.D., Mathematical model of fetal circulation and oxygen delivery (1985) American Journal of Physiology, 249, pp. R192-R202; 
Itskovitz, J., LaGamma, E., Rudolph, A.M., The effect of reducing umbilical blood flow on fetal oxygenation (1983) American Journal of Obstetric and Gynecology, 145 (7), pp. 813-818; 
Polin, R.A., Fox, W.W., (1998) Fetal and Neonatal Physiology, , (2nd ed.). London: W.B. Saunders Company; 
Schwid, H.A., Flight simulator for general anesthesia training (1987) Computers and Biomedical Research, 20, pp. 64-75; 
Schwid, H.A., O{\textquoteright}Donnell, D., The anesthesia simulator- recorder: A device to train and evaluate anesthesiologists{\textquoteright} responses to critical incidents (1990) Anesthesiology, 72, pp. 191-197; 
Severinghaus, J.W., Simple, accurate equations for human blood O 2 dissociation computations (1979) Journal of Applied Physiology, 46 (3), pp. 599-602; 
Skillman, C.A., Plemessinger, M.A., Woods, J.R., Clark, K.E., The effect of graded reductions in uteroplacental blood flow on fetal lamb (1985) American Journal of Physiology, 249 (18), pp. H1098-H1105; 
Sukumar, N., Morin III, F.C., Response of the fetal circulation to stress (1998) Fetal and Neonatal Physiology, pp. 1014-1023. , (2nd ed.) London: WB Saunders Company; 
van Meurs, W.L., Good, M., Lampotang, S., Functional anatomy of full-scale patient simulators (1997) Journal of Clinical Monitoring, 13 (5), pp. 317-324; 
Zwart, A., Smith, N.T., Beneken, J.E.W., Multiple model approach to uptake and distribution of halothane: The use of an analog computer (1972) Computers and Biomedical Research, 5, pp. 228-238
},
	month = {2002///},
	pages = {59 - 66},
	abstract = {Critical situations in obstetrics and anesthesia of the pregnant woman are rare and associated with a high risk to the woman and fetus involved. Therefore, simulation is a valuable tool in teaching the diagnostic and therapeutic skills in this context. We derive a mathematical model for the oxygen supply to the fetus that was specifically designed for educational simulations. The model reflects the hemodynamics and the oxygen transport. Parameter values for human patients are derived from the literature data. We validate the response of the model, with parameters reflecting the fetal lamb, to various reductions of the uterine and umbilical blood flow. {\textcopyright} 2002 Elsevier Science Ltd. All rights reserved.},
	keywords = {Anesthesiology, Blood, Computer simulation, control, Diagnosis, education, Fetal monitoring, fetus, hemodynamics, mathematical model, Mathematical models, medical application, Obstetrics, oxygen, Oxygen supply, simulator, Therapeutic skills},
	isbn = {09670661 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036141420\&partnerID=40\&md5=5b294e0689357e03c548b7ccf608b476},
	author = {S{\'a} Couto, P.M. and van Meurs, W.L. and Bernardes, J.F. and Marques De S{\'a}, J.P. and Goodwin, J.A.}
}
@article {2527,
	title = {Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima: A new insight in the biomineralization field},
	journal = {European Journal of BiochemistryEur. J. Biochem.},
	volume = {269},
	year = {2002},
	note = {Cited By (since 1996): 58Export Date: 21 November 2012Source: ScopusCODEN: EJBCAdoi: 10.1046/j.1432-1033.2002.03203.xPubMed ID: 12383258Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiologie Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Amino Acids; Calcium, 7440-70-2; Edetic Acid, 60-00-4; Extracellular Matrix Proteins; Glycosaminoglycans; Insect Proteins; SilkReferences: Crenshaw, M.A., The soluble matrix from Mercenaria mercenaria shell (1972) Biomineralization, 6, pp. 6-11;Krampitz, G., Engels, J., Cazaux, C., Biochemical studies on water-soluble proteins and related components of gastropod shells (1976) The Mechanisms of Mineralization in the Invertebrates and Plants, pp. 175-190. , Watabe, N. \& Wilbur, K.M., eds. The Barush B.W. Library in Marine Science no. 5, The University of South Carolina Press, Columbia; 
Weiner, S., Traub, W., Lowenstam, H.A., Organic matrix in calcified exoskeletons (1983) Biomineralization and Biological Metal Accumulation, pp. 205-224. , Westbroek, P. \& deJong, E.W., eds. Reidel, Dordrecht, Holland; 
Keith, J., Stockwell, D., Ball, D., Remillard, K., Kaplan, D., Thannhauser, T., Sherwood, R., Comparative analysis of macromolecules in mollusc shells (1993) Comp. Biochem. Physiol., 105 B, pp. 487-496; 
Levi-Kalisman, Y., Falini, G., Addadi, L., Weiner, S., Structure of the nacreous organic matrix of a bivalve mollusk shell examined in the hydrated state using cryo-TEM (2001) J. Struct. Biol., 135, pp. 8-17; 
Weiner, S., Hood, L., Soluble protein of the organic matrix of mollusk shells: A potential template for shell formation (1975) Science, 190, pp. 987-989; 
Weiner, S., Traub, W., Macromolecules in mollusc shells and their functions in biomineralization (1984) Phil. Trans. R. Soc. Lond. B, 304, pp. 425-434; 
Belcher, A.M., Wu, X.H., Christensen, R.J., Hansma, P.K., Stucky, G.D., Morse, D.E., Control of crystal phase switching and orientation by soluble mollusc-shell proteins (1996) Nature, 381, pp. 56-58; 
Kamat, S., Su, X., Ballarini, R., Heuer, A.H., Structural basis for the fracture toughness of the shell of the conch Strombus gigas (2000) Nature, 405, pp. 1036-1040; 
Hare, P.E., Amino acids in the proteins from aragonite and calcite in the shells of Mytilus californianus (1963) Science, 139, pp. 216-217; 
Hare, P.E., Abelson, P.H., Amino acid composition of some calcified proteins (1965) Carnegie Inst. Washington Year Book, 64, p. 223; 
Watabe, N., Shell (1984) Biology of the Integument, 1, pp. 448-485. , Invertebrates (Bereiterhahn, J., Matolsty, A.G. \& Richards, K.S., eds). Springer-Verlag, Berlin; 
Mann, S., Molecular recognition in biomineralization (1988) Nature, 332, pp. 119-124; 
Kono, M., Hayashi, N., Samata, T., Molecular mechanism of the nacreous layer formation in Pinctada maxima (2000) Biochem. Biophys. Res. Commun., 269, pp. 213-218; 
Lopez, E., Berland, S., Le Faou, A., Nacre, osteogenic and osteoinductive properties (1994) Bull. Inst. Oc{\'e}an Monaco, 14, pp. 49-57; 
Lopez, E., Vidal, B., Berland, S., Camprasse, C., Camprasse, G., Silve, C., Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro (1992) Tissue Cell, 24, pp. 667-679; 
Atlan, G., Balmain, N., Berland, S., Vidal, B., Lopez, E., Reconstruction of human maxillary defects with nacre powder: Histological evidence for bone regeneration (1997) C. R. Acad. Sci. Paris, Life Sci., 320, pp. 253-258; 
Atlan, G., Delattre, O., Berland, S., Le Faou, A., Nabias, G., Cot, D., Lopez, E., Interface between bone and nacre implants in sheep (1999) Biomaterials, 20, pp. 1017-1022; 
Lamghari, M., Almeida, M.J., Berland, S., Huet, H., Laurent, A., Milet, C., Lopez, E., Stimulation of bone marrow cells and bone formation by nacre: In vivo and in vitro studies (1999) Bone, 25 S, pp. 91S-94S; 
Almeida, M.J., Milet, C., Peduzzi, J., Pereira, L., Haigle, J., Barth{\'e}lemy, M., Lopez, E., The effect of water-soluble matrix fraction extracted from the nacre of Pinctada maxima on the alkaline phosphatase activity of cultured fibroblasts (2000) J. Exp. Zool. (Mol. Dev. Evol.), 288, pp. 327-334; 
Almeida, M.J., Pereira, L., Milet, C., Haigle, J., Barbosa, M., Lopez, E., Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts (2001) J. Biomed. Mater. Res., 57, pp. 306-312; 
Lamghari, M., Berland, S., Laurent, A., Huet, H., Lopez, E., Bone reactions to nacre injected percutaneously into the vertebrae of sheep (2001) Biomaterials, 22, pp. 555-562; 
Lopez, E., Giraud, M., Le Faou, A., Berland, S., Gutierrez, J., (1995), Proc{\'e}d{\'e} de pr{\'e}paration de substances actives {\`a} partir de la nacre, produits obtenus, utiles comme m{\'e}dicaments. Patent CNRS no. FR. 9515650, 28/12/1995, WO 97/24133-10/07/97Wheeler, A.P., Rusenko, K.W., George, J.W., Sikes, S., Evaluation of calcium binding by molluscan shell organic matrix and its relevance to biomineralization (1987) Comp. Biochem. Physiol., 87 B, pp. 953-960; 
Cohen-Solal, L., Lian, J.B., Kossiva, D., Glimcher, M.J., The identification of O-phosphothreonine with soluble non-collagenous phosphoproteins of bone matrix (1978) FEBS Lett., 89, pp. 107-110; 
Cohen, S.A., Strydom, D.J., Amino acid analyses utilizing phenylisothiocyanate derivatives (1988) Anal. Biochem., 174, pp. 1-16; 
Hewitt, B.R., Spectrophotometric determination of protein in alkaline solution (1958) Nature, 187, p. 246; 
Whiteman, P., The quantitative determination of glycosaminoglycans in urine with Alcian blue 8GX (1973) Biochem. J., 131, pp. 351-357; 
Whiteman, P., The quantitative measurement of Alcian blue-glycosaminoglycan complexes (1973) Biochem. J., 131, pp. 343-350; 
Gold, E.W., A simple spectrophotometric method for estimating glycosaminoglycan concentrations (1979) Anal. Biochem., 99, pp. 183-188; 
Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685; 
Morrissey, J.H., Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity (1981) Anal. Biochem., 117, pp. 307-310; 
Wheeler, A.P., Rusenko, K.W., Sikes, S., Organic matrix from carbonate biomineral as a regulator of mineralization (1988) Chemical Aspects of Regulation of Mineralization, , (Sikes, C.S. \& Wheeler, A.P., eds). University of South Alabama Publication Services, Mobile, Alabama; 
Weiner, S., Aspartic acid-rich proteins: Major components of the soluble organic matrix of mollusk shells (1979) Calcif. Tissue Int., 29, pp. 163-167; 
Wheeler, A.P., Rusenko, K.W., Swift, D.M., Sikes, S., Regulation of in vitro and in vivo CaCO 3 crystallization by fractions of oyster shell organic matrix (1988) Mar. Biol., 98, pp. 71-80; 
Halloran, B.A., Donachy, J.E., Characterization of organic matrix macromolecules from the shells of the antarctic scallop Adamussium colbecki (1995) Comp. Biochem. Physiol., 111 B, pp. 221-231; 
Scott, J.E., Quintarelli, G., Dellovo, M.C., The chemical and histochemical properties of Alcian blue staining (1964) Histochemie, 4, pp. 73-85; 
Nakamoto, K., (1986) IR and Raman Spectra of Inorganic and Coordination Compounds, , J. Wiley \& Sons, New York; 
Samata, T., Krampitz, G.P., Calcium binding polypeptides in oyster shells (1982) Malacologia, 22, pp. 225-233; 
Roeges, N.P.G., (1998) A Guide to the Complete Interpretation of Infrared Spectra of Organic Structures, , J. Wiley \& Sons, New York; 
Cariolou, M., Morse, D., Purification and characterization of calcium binding conchiolin shell peptides from the mollusc, Haliotis rufescens, as a function of development (1988) J. Comp. Physiol., 157 B, pp. 717-729; 
Krampitz, G.P., Structure of the organic matrix in mollusc shells and avian eggshells (1982) Biological Mineralization and Demineralization, pp. 219-232. , Nancollas, G.H., ed. Springer-Verlag, Berlin, Heidelberg, New York; 
Weiner, S., Addadi, L., Acidic macromolecules of mineralized tissues: The controllers of crystal formation (1991) Trends Biochem. Sci., 16, pp. 252-256; 
Marin, F., Pereira, L., Westbroek, P., Large-scale fractionation of molluscan shell matrix (2001) Protein Expr. Purif., 23, pp. 175-179; 
Balmain, J., Hannoyer, B., Lopez, E., Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analyses of mineral and organic matrix during heating of mother of pearl (nacre) from the shell of the mollusc Pinctada maxima (1999) J. Biomed. Mater. Res. Appl. Mat., 48, p. 754; 
Marxen, J.C., Hammer, M., Gehrke, T., Becker, W., Carbohydrates of the organic shell matrix and the shell forming tissue of the snail Biomphalaria glabrata (Say) (1998) Biol. Bull., 194, pp. 231-240; 
Miyazawa, T., Blout, E.R., The infrared spectra of polypeptides in various conformations - Amide I and II bands (1961) J. Am. Chem. Soc., 83, pp. 712-719; 
Worms, D., Weiner, S., Mollusk shell organic matrix: Fourier transform infrared study of the acidic macromolecules (1986) J. Exp. Zool., 237, pp. 11-20; 
Weiner, S., Traub, W., X-ray diffraction study of the insoluble organic matrix of mollusk shells (1980) FEBS Lett., 111, pp. 311-316; 
Pereira-Mouri{\`e}s, L., Almeida, M.J., Marin, F., Gay, M., Milet, C., Lopez, E., Soluble proteins of the nacre from the giant oyster Pinctada maxima with highly hydrophobic amino acids (2002) Biomineralization: Formation, Diversity, Evol. Appl., , in press; 
Nakahara, H., Kakei, M., Bevelander, G., Fine structure and amino acid composition of the organic {\textquoteright}envelope{\textquoteright} in the prismatic layer of some bivalve shells (1980) Venus Jpn. J. Malacol., 39, pp. 167-177; 
Falini, G., Albeck, S., Weiner, S., Addadi, L., Control of aragonite or calcite polymorphism by mollusk shell macromolecules (1996) Science, 271, pp. 67-69; 
Simkiss, K., The organic matrix of the oyster shell (1965) Comp. Biochem. Physiol., 16, pp. 427-435; 
Golberg, M., Takagi, M., Dentine proteoglycans: Composition, ultrastructure, and functions (1993) Histochem. J., 25, pp. 781-806; 
Wilbur, K.M., Recent studies of invertebrate mineralization (1976) The Mechanisms of Mineralization in Invertebrates and Plants, pp. 79-108. , Watabe, N. \& Wilbur, K.M., eds. The University of South Carolina Press, The Belle W. Baruch Library in Marine Science no. 5, Columbia; 
Addadi, L., Moradian, J., Shay, E., Maroudas, N.G., Weiner, S., A chemical model for the cooperation of sulfates and carboxylates in calcite crystal nucleation: Revelance to biomineralization (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 2732-2736; 
Marxen, J.C., Becker, W., Calcium binding constituents of the organic shell matrix from the freshwater snail Biomphalaria glabrata (2000) Comp. Biochem. Physiol., 127 B, pp. 235-242; 
Tanaka, Y., Adams, D.H., Shaw, S., Proteoglycans on endothelial cells present adhesion-inducing cytokines to leukocytes (1993) Immunol. Today, 14, pp. 111-115; 
Jackson, R.L., Bush, S.J., Cardin, A.D., Glycosaminoglycans: Molecular properties, protein interactions, and role in physiological processes (1991) Physiol. Rev., 71, pp. 481-539; 
Nakahara, H., Calcification of gastropod nacre (1983) Biomineralization and Biological Metal Accumulation, pp. 225-230. , Westbroek, P. \& deJong, E.W., eds. Reidel, Dordrecht, Holland; 
Zentz, F., B{\'e}douet, L., Almeida, M.J., Milet, C., Lopez, E., Giraud, M., Characterization and quantification of chitosan extracted from nacre of the abalone Haliotis tuberculata and the oyster Pinctada maxima (2001) Mar. Biotechnol., 3, pp. 36-44; 
Alzieu, C., Thibaud, Y., Heral, M., Boutier, B., Evaluation des risques dus a l{\textquoteright}emploi des peintures anti-sallissures dans les zones conchyoliques (1980) Rev. Trav. Inst. P{\^e}ches Marit., 44, pp. 301-348; 
Alzieu, C., Heral, M., Thibaud, Y., Dardignac, M.J., Feuillet, M., Influence des peintures anti-sallissures {\`a} base d{\textquoteright}organostanniques sur la calcification de la coquille de l{\textquoteright}hu{\^\i}tre Crassostrea gigas (1982) Rev. Trav. Inst. P{\^e}ches Marit., 45, pp. 101-116; 
Krampitz, G., Drolshagen, H., Deltreil, J.P., Soluble matrix components in malformed oyster shells (1983) Experientia, 39, pp. 1105-1106; 
Alzieu, C., Heral, M., Ecotoxicological effects of organotin coumpounds on oyster culture (1984) Ecotoxicological Testing for the Marine Environment, 2, pp. 187-196. , Persoone, G., Jaspers, E. \& Claus, C., eds. State University Ghent and Inst. Mar. Sci. Res., Bredene, Belgium; 
Almeida, M.J., (1996) Crescimento em Ostras: Factores que Afectam a Forma{\c c}{\~a}o e Estrutura da Concha, , PhD Thesis, Instituto de Ci{\^e}ncias Biom{\'e}dicas Abel Salazar, University of Porto, Portugal; 
Sudo, S., Fujikawa, T., Nagakura, T., Ohkubo, T., Sakaguchi, K., Tanaka, M., Nakashina, K., Takahashi, T., Structures of mollusc shell framework proteins (1997) Nature, 387, pp. 563-564; 
Shen, X., Belcher, A.M., Hansma, P.K., Stucky, G.D., Morse, D.E., Molecular cloning and characterization of Lustrin A, a matrix protein from shell and pearl nacre of Haliotis rufescens (1997) J. Biol. Chem., 272, pp. 32472-32481; 
Samata, T., Hayashi, N., Kono, M., Hasegawa, K., Horita, C., Akera, S., A new matrix protein family related to the nacreous layer formation of Pinctada fucata (1999) FEBS Lett., 462, pp. 225-229; 
Mann, S., Webb, J., Williams, R.J.P., (1989) Biomineralization: Chemical and Biochemical Perspectives, pp. 35-62. , VCH, Weinheim; 
Simkiss, K., Wilbur, K.M., (1989) Biomineralization - Cell Biology and Mineral Deposition, , Academic Press, San Diego; 
Sarashina, I., Endo, K., Primary structure of a soluble matrix protein of scallop shell: Implications for calcium carbonate biomineralization (1998) Am. Mineral., 83, pp. 1510-1515; 
Weiss, I., Kaufmann, S., Mann, K., Fritz, M., Purification and characterization of perlucin and perlustrin, two new proteins from the shell of the mollusc Haliotis laevigata (2000) Biochem. Biophys. Res. Commun., 267, pp. 17-21; 
Pereira-Mouri{\`e}s, L., Milet, C., Almeida, M.J., Rousseau, M., Borzeix, S., Lopez, E., Biological effect of the water-soluble matrix extract of the nacre shell layer of Pinctada maxima on vertebrate cell proliferation and differentiation (2001) Proceedings of the 3rd International Satellite Symposium on the Comparative Endocrinology of Calcium Regulation, , http://www.sci.port.ac.uk/-cecr, Gay, C.V., Dacke, C.G., Dancks, J.A. \& Cox, P.A., eds; 
Pereira-Mouri{\`e}s, L., Almeida, M.J., Milet, C., Berland, S., Lopez, E., Bioactivity of nacre water-soluble organic matrix from the bivalve mollusk Pinctada maxima in three mammalian cell types: Fibroblasts, bone marrow stromal cells and osteoblasts (2002) Comp. Biochem. Physiol., 132, pp. 215-217
},
	month = {2002///},
	pages = {4994 - 5003},
	abstract = {Nacre organic matrix has been conventionally classified as both {\textquoteright}water-soluble{\textquoteright} and {\textquoteright}water-insoluble{\textquoteright}, based on its solubility in aqueous solutions after decalcification with acid or EDTA. Some characteristics (aspartic acid-rich, silk-fibroin-like content) were specifically attributed to either one or the other. The comparative study on the technique of extraction (extraction with water alone vs. demineralization with EDTA) presented here, seems to reveal that this generally accepted classification may need to be reconsidered. Actually, the nondecalcified soluble organic matrix, extracted in ultra-pure water, displays many of the characteristics of what until now has been called {\textquoteright}insoluble matrix{\textquoteright}. We present the results obtained on this extract and on a conventional EDTA-soluble matrix, with various characterization methods: fractionation by size-exclusion and anion-exchange HPLC, amino acid analysis, glycosaminoglycan and calcium quantification, SDS/PAGE and FTIR spectroscopy. We propose that the model for the interlamellar matrix sheets of nacre given by Nakahara [In: Biomineralization and Biological Metal Accumulation, Westbroek, P. \& deJong, E.W., eds, (1983) pp. 225-230. Reidel, Dordrecht, Holland] and Weiner and Traub [Phil. Trans. R. Soc. Lond. B (1984) 304, 425-434] may no longer be valid. The most recent model, proposed by Levi-Kalisman et al. [J. Struct. Biol. (2001) 135, 8-17], seemed to be more in accordance with our findings.},
	keywords = {amino acid analysis, Amino acids, Animals, anion exchange chromatography, article, Biochemistry, biomineralization, bivalve, Bivalvia, Calcium, Chemical Fractionation, Chromatography, High Pressure Liquid, controlled study, demineralization, edetic acid, EDTA-soluble matrix, Electrophoresis, Polyacrylamide Gel, Extracellular Matrix Proteins, extraction, fractionation, gel permeation chromatography, glycosaminoglycan, Glycosaminoglycans, High performance liquid chromatography, hydrophobicity, infrared spectroscopy, Insect Proteins, model, Nacre, nonhuman, Ostreidae, Pinctada, Pinctada maxima, polyacrylamide gel electrophoresis, priority journal, Silk, Silk-fibroin-like-proteins, Solubility, Spectroscopy, Fourier Transform Infrared, Undecalcified soluble matrix, Water},
	isbn = {00142956 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036408881\&partnerID=40\&md5=7e99def46be711f02de5f4dd3dc8f63e},
	author = {Pereira-Mouri{\`e}s, L. and Almeida, M.-J. and Ribeiro, C. and Peduzzi, J. and Barth{\'e}lemy, M. and Milet, C. and Lopez, E.}
}
@article {2544,
	title = {Adsorption and release studies of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites},
	journal = {BiomaterialsBiomaterials},
	volume = {22},
	year = {2001},
	note = {Cited By (since 1996): 55Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(00)00296-9PubMed ID: 11336313Language of Original Document: EnglishCorrespondence Address: Knowles, J.C.; Department of Biomaterials, Eastman Dental Institute, University College London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United Kingdom; email: j.knowles@eastman.ucl.ac.ukChemicals/CAS: Ampicillin, 69-53-4; Durapatite, 1306-06-5; PenicillinsManufacturers: Plasma Biotal, United KingdomReferences: Farag, S.A., Simultaneous liquid chromatographic analysis of the β-lactam antibiotics cefazolin, cefadroxil, cepalexin, ampicillin, and cephradine in solution (1998) J AOAC Int, 81, pp. 381-385;Somayaji, B.V., Jariwala, U., Jayachandran, P., Vidyalakshmi, K., Dudhani, R.V., Evaluation of antimicrobial efficacy and release pattern of tetracycline and metronidazole using a local delivery system (1998) J Periodontol, 69, pp. 409-413; 
Soskolne, W.A., Heasman, P.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis; a multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Steinberg, D., Friedman, M., Soskolne, A., Sela, M.N., A new degradable controlled release device for treatment of periodontal disease; in vitro release study (1990) J Periodontol, 61, pp. 393-398; 
Stoltze, K., Concentration of metronidazole in periodontal pockets after application of a metronidazole 25\% dental gel (1992) J Clin Periodontol, 19, pp. 698-701; 
Ramaglia, L., Sbordone, L., Lenci, F., Gulletta, E., Amato, G., Kennedy, E.B., Microbiology of porous hydroxylapatite implants in human periodontal osseous defects (1986) Biomaterials and clinical applications, 7 vol., pp. 675-680. , Pizzoferrate A., Marachetti P.G., Ravaglioli A., Lee A.J.C. (Eds.), Netherlands: Elsevier; 
Wilson, J., Low, S., Fetner, A., Hench, L.L., Bioactive materials for periodontal treatment; a comparative study (1986) Biomaterials and clinical applications, 7 vol., pp. 223-228. , Pizzoferrate A., Marachetti P.G., Ravaglioli A., Lee A.J.C. (Eds.), Netherlands: Elsevier; 
Kenney, E.B., Lekovic, V., Sa Ferreira, J.C., Han, T., Dimitrijavic, B., Carranza F.A. Jr, Bone formation within porous hydroxyapatite implants in human periodontal defects (1986) J Periodontol, 57, pp. 76-83; 
Krejei, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical evaluation of porous and nonporous hydroxyapatite in treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., Reinforcement of hydroxyapatite by adding P 2O 5-CaO glasses with Na 2O, K 2O, MgO (1996) J Mater Sci Mater Med, 7, pp. 187-189; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Osteocompatibility and in vivo evaluation of glass reinforced hydroxyapatite composites (1999) Bioceramics, 12 vol., pp. 421-424. , Ohgushi H., Hastings G.W., Yoshikawa T. (Eds.), Japan: World Scientific; 
Santos, J.D., Vasconcelos, M., Reis, R.L., Afonso, A., Monteiro, F.J., Hastings, G.W., Glass reinforced hydroxyapatite composites; physical properties and preliminary histological studies in rabbits (1994) Bioceramics, 7 vol., pp. 243-248. , Andersson O.H., Yli-Urpo A. (Eds.), Finland: Pergamon Press; 
Klein, C.P.A.T., Wolke, J.G.C., de Blieck-Hogervorst, J.M.A., de Groot, K., Features of calcium phosphate plasma-sprayed coatings; an in vitro study (1994) J Biomed Mater Res, 28, pp. 961-967; 
Klein, C.P.A.T., Wolke, J.G.C., de Blieck-Hogervorst, J.M.A., de Groot, K., Calcium phosphate plasma-sprayed coatings and their stability; an in vivo study (1994) J Biomed Mater Res, 28, pp. 909-917; 
Langnbucher, F., Benz, D., K{\"u}rth, W., M{\"o}ller, H., Otz, M., Standardized Flow-cell method as an alternative to existing pharmacopoeial dissolution testing (1989) Pharm Ind, 51, pp. 1276-1281; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterization of glass-reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass-reinforced hydroxyapatite; a comprehensive study of the effect of glass composition on crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Thomas M. Ultraviolet and visible spectroscopy, second edition. In: Ando DJ, editor. England: Wiley, 1996. p. 16-30Itokazu, M., Itoh, Y., Fukuta, M., Miyamato, K., Ohara, A., Oshima, K., Nishimoto, Y., Shimizu, K., Treatment of osteomyelitis by using antibiotic loaded porous ceramic (1999) Bioceramics, 12 vol., pp. 3-6. , Ohgushi H., Hastings G.W., Yoshikawa T. (Eds.), Japan: World Scientific; 
Shinto, Y., Uchida, A., Korkusuz, F., Araki, N., Ono, K., Calcium hydroxyapatite ceramic used as a delivery system for antibiotics (1992) J Bone Jt Surg, 74 B, pp. 600-604; 
Downes, S., Clifford, C.J., Scotchford, C., Klein, C.P.A.T., Comparison of the release of growth hormone from hydroxyapatite, heat-treated hydroxyapatite, and fluoroapatite coatings on titanium (1995) J Biomed Mater Res, 29, pp. 1053-1060; 
Curtis, M.A., Griffiths, G.S., Johnson, N.W., The total protein concentration of gingival crevicular fluid. Variation with sampling time and gingival inflammation (1988) J Clin Periondontol, 15, pp. 628-632; 
Stoltze K. Concentration of metronidazole in periodontal pockets after application of a metronidazole 25\% dental gel. J Clin Periondontol 1992, 689-701Jones, A.A., Koenman, K.S., Newbold, D.A., Manwell, M.A., Clinical and microbiological effects of controlled release locally delivered minocycline in periodontitis (1994) J Periodontol, 65, pp. 1058-1066
},
	month = {2001///},
	pages = {1393 - 1400},
	abstract = {As a potential therapy for periodontitis, sodium ampicillin, a broad spectrum antibiotic, was adsorbed onto hydroxyapatite (HA) and glass-reinforced hydroxyaptite (GR-HA) composites, and was subsequently released in vitro. The sodium ampicillin, was adsorbed more on HA compared to the GR-HA composites. X-ray diffraction (XRD) and Rietveld analysis were used to identify and quantify the levels of HA and β-tricalcium phosphate (β-TCP) in the microstructure of the GR-HA composites. Lattice parameters changes were observed for the β-TCP phase dependant on the amount of glass added. The release kinetics were shown to be divided into three stages, the first of which where a large amount of sodium ampicillin is released, followed by a slower release rate and then a final stage where the release amount approaches zero, until no more sodium ampicillin was present. X-ray photoeletron spectroscopy (XPS) studies were carried out in order to ensure that the entire antibiotic adsorbed onto the materials had been released. These kinetics studies have indicated the possibility of using these materials as possible carriers for drug delivery. Copyright {\textcopyright} 2001 Elsevier Science Ltd.},
	keywords = {Adsorption, ampicillin, Antibiotics, article, calcium phosphate, composite material, controlled study, drug adsorption, Drug delivery, drug delivery system, Drug release, Durapatite, glass, Glass-reinforced hydroxyapatite, hydroxyapatite, Kinetics, Microstructure, Penicillins, periodontitis, priority journal, Reinforcement, Rietveld analysis, roentgen spectroscopy, Sodium ampicillin, Sodium compounds, structure analysis, unclassified drug, X ray diffraction, X ray diffraction analysis, X ray photoelectron spectroscopy, X-Ray Diffraction},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035372020\&partnerID=40\&md5=72e1b530ae04faed9cceb376c0246aa8},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J. and Gibson, I.R. and Knowles, J.C.}
}
@article {2540,
	title = {Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {57},
	year = {2001},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/1097-4636(200111)57:2<306::AID-JBM1172>3.0.CO;2-HPubMed ID: 11484195Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiol. Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; BMP2 protein, human; Bmp2 protein, rat; bone morphogenetic protein 2; Bone Morphogenetic Proteins; Dexamethasone, 50-02-2; Glucocorticoids; Transforming Growth Factor betaReferences: Lopez, E., Vidal, B., Berland, S., Camprasse, S., Camprasse, G., Silve, C., Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro (1992) Tissue Cell, 24, pp. 667-679;Lopez, E., Berland, S., Le Faou, A., Nacre, osteogenic and osteoinductive properties (1994) Bull Inst Oc{\'e}an Monaco, 14, pp. 49-57; 
Silve, C., Lopez, E., Vidal, B., Smith, D.C., Camprasse, S., Camprasse, G., Couly, G., Nacre initiates biomineralization by human osteoblasts maintained in vitro (1992) Calcif Tissue Int, 51, pp. 363-369; 
Atlan, G., Balmain, N., Berland, S., Vidal, B., Lopez, E., Reconstruction of human maxillary defects with nacre powder: Histological evidence for bone regeneration (1997) C R Acad Sci III, 320, pp. 253-258; 
Atlan, G., Delattre, O., Berland, S., Le Faou, A., Nabias, G., Cot, D., Lopez, E., Interface between bone and nacre implants in sheep (1999) Biomaterials, 20, pp. 1017-1022; 
Lamghari, M., Almeida, M.J., Berland, S., Huet, H., Laurent, A., Milet, C., Lopez, E., Stimulation of bone marrow cells and bone formation by nacre: In vivo and in vitro studies (1999) Bone, 25 S, pp. 91S-94S; 
Westbroek, P., Marin, F., A marriage of bone and nacre (1998) Nature, 392, pp. 861-862; 
Le Faou, A., Berland, S., Lallier, F., Lopez, E., Osteoinductive effects induced by nacre implanted in subcutaneous pouches in rat (1995) Calcif Tissue Int, 56 A, p. 156; 
Le Faou, A., Berland, S., Lallier, F., Lopez, E., Local regulation of the osteoinductive process induced by nacre in connective tissues (1996) Ann Endoc, 57 (SUPPL. 4A), p. 75; 
Aubin, J.E., Liu, F., Malaval, L., Gupta, A.K., Osteoblast and chondroblast differentiation (1995) Bone, (SUPPL. 2), pp. S77-S83; 
Huggins, C.B., Urist, M.R., Dentin matrix transformation: Rapid induction of alkaline phosphatase and cartilage (1970) Science, 167, pp. 896-897; 
Reddi, A.H., Huggins, C.B., Biochemical sequences in the transformation of normal fibroblasts in adolescent rats (1972) Proc Natl Acad Sci USA, 69, pp. 1601-1605; 
Glowacki, J., Mulliken, J.B., Demineralized bone implants (1985) Clin Plast Surg, 12 (2), pp. 233-241; 
Katagiri, T., Yamaguchi, A., Ikeda, T., Yoshiki, S., Wozney, J.M., Rosen, V., Wang, E.A., Suda, T., The nonosteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteobtastic cells by recombinant human bone morphogenetic protein-2 (1990) Biochem Biophys Res Commun, 172, pp. 295-299; 
McCulloch, C.A.G., Tenenbaum, H.C., Dexamethasone induces proliferation and terminal differentiation of osteogenic cells in tissue culture (1986) Anat Rec, 215, pp. 397-402; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Bellows, C.G., Aubin, J.E., Determination of numbers of osteoprogenitors present in isolated fetal calvarial cells in vitro (1989) Dev Biol, 133, pp. 8-13; 
Bhargava, U., Bar-Lev, M., Bellows, C.G., Aubin, J.E., Ultrastructural analyses of bone nodules formed in vitro by isolated fetal rat calvaria cells (1988) Bone, 9, pp. 155-163; 
Wiren, K.M., Rodan, G.A., Dissociation between the effects of glucocorticosteroids on cell spreading and phenotypic expression (1985) The chemistry and biology of mineralized tissues, pp. 320-325. , Butler WT, editor. Birmingham, AL: EBSCO Media; 
Kasugai, S., Todescan, R., Nagata, T., Yao, K.-L., Butler, W.T., Sodek, J., Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro: Inductive effects of dexamethasone on the osteoblastic phenotype (1991) J Cell Physiol, 147, pp. 111-120; 
Chen, T.L., Bates, R.L., Dudley, A., Hammonds, R.G., Amento, E.P., Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteobtast-like cells: Comparison with TGF-β1 (1991) J Bone Miner Res, 6, pp. 1387-1393; 
Hiraki, Y., Inoue, H., Shigeno, C., Sanma, Y., Bentz, H., Rosen, D.M., Asada, A., Suzuki, F., Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitro (1991) J Bone Miner Res, 6, pp. 1373-1385; 
Rosen, V., Thies, R.S., The BMP proteins in bone formation and repair (1992) Trends Genet, 8, pp. 97-102; 
Thies, R.S., Bauduy, M., Ashton, B.A., Kurtzberg, L., Wozney, J.M., Rosen, V., Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells (1992) Endocrinology, 130, pp. 1318-1324; 
Urist, M.R., Iwata, H., Ceccotti, P.L., Dorfman, R.L., Boyd, S.D., McDowell, R., Chien, C., Bone morphogenesis in implants of insoluble bone gelatin (1973) Proc Natl Acad Sci USA, 70, pp. 3511-3515; 
Almeida, M.J., Milet, C., Peduzzi, J., Pereira, L., Haigle, J., Barth{\'e}lemy, M., Lopez, E., The effect of water-soluble matrix fraction extracted from the nacre of Pinctada maxima on the alkaline phosphatase activity of cultured fibroblasts (2000) J Exp Zool, 288, pp. 327-334; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Owen, M., Marrow stromal stem cells (1988) J Cell Sci, (SUPPL. 10), pp. 63-76; 
Triffitt, J.T., Initiation and enhancement of bone formation (1987) Acta Orthop Scand, 58, pp. 673-684; 
Triffitt, J.T., The stem cell of the osteoblast (1996) Principles of bone biology, pp. 39-50. , Bilezikian JP, Raisz LG, Rodan GA, editors. New York: Academic Press; 
Wharton, S.A., Hipkiss, A.R., Degradation of peptides and proteins of different sizes by homogenates of human MRC5 lung fibroblasts (1985) FEBS Lett, 184 (2), pp. 249-253; 
Harada, K., Oida, S., Sasaki, S., Chondrogenesis and osteogenesis of bone marrow-derived cells by bone-inductive factor (1988) Bone, 9, pp. 177-183; 
Tucker, R.F., Shipley, G.D., Moses, H.L., Growth inhibitor from BSC-1 cells closely related to platelet type β transforming factor (1984) Science, 226, pp. 705-707; 
Noda, M., Rodan, G.A., Type-β transforming growth factor inhibits proliferation and expression of alkaline phosphatase in murine osteoblast-like cells (1986) Biochem Biophys Res Commun, 140, pp. 56-65; 
Tintut, Y., Parhami, F., Bostr{\"o}m, K., Jackson, S., Demer, L., cAMP stimulates osteoblast-like differentiation of calcifying vascular cells (1998) J Biol Chem, 273, pp. 7547-7553; 
Rickard, D.J., Sullivan, T.A., Shenker, B.J., Leboy, P.S., Kazhdan, I., Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2 (1994) Dev Biol, 161, pp. 218-228; 
Coelho, M.J., Fernandes, M.H., Human bone cell cultures in biocompatibility testing. Part II. Effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation (2000) Biomaterials, 21, pp. 1095-1102; 
Green, S., Chambon, P., A superfamily of potentially oncogenic hormone receptors (1986) Nature, 324, pp. 615-617; 
Grigoriadis, A.E., Heersche, J.N.M., Aubin, J.E., Differentiation of muscle, fat, cartilage and bone from progenitor cells present in a bone-derived clonal cell population: Effect of dexamethasone (1988) J Cell Biol, 106, pp. 2139-2151; 
Leboy, P.S., Beresford, J.N., Devlin, C., Owen, M.E., Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures (1991) J Cell Physiol, 146, pp. 370-378; 
Guerriero V., Jr., Florini, J.R., Dexamethasone effects on myoblast proliferation and differentiation (1980) Endocrinology, 106, pp. 1198-1202; 
Maor, G., Silbermann, M., Supraphysiological concentrations of dexamethasone induce elevation of calcium uptake and depression of [ 3H]-thymidine incorporation into DNA in cartilage in vitro (1986) Calcif Tissue Int, 39, pp. 284-290; 
Urist, M.R., Bone morphogenetic protein in biology and medicine (1996) Bone morphogenetic proteins: Biology, biochemistry and reconstructive surgery, pp. 7-27. , Lindholm TS, editor. Austin, TX: RG Landes Company; 
Wozney, J.M., Rosen, V., Byrne, T., Celeste, A.J., Moutsatsos, I., Wang, E.A., Growth factor influencing bone development (1990) J Cell Sci, 97 (SUPPL. 13), pp. 149-156; 
Takuwa, Y., Ohse, C., Wang, E.A., Wozney, J.M., Yamashita, K., Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3 (1991) Biochem Biophys Res Commun, 174, pp. 96-101; 
Jortikka, L., Target cells and receptors for bone morphogenetic protein (1996) Bone morphogenetic proteins: Biology, biochemistry and reconstructive surgery, pp. 65-73. , Lindholm TS, editor. Austin, TX: RG Landes Company; 
Canalis, E., Centrella, M., Urist, M.R., Effect of partially purified bone morphogenetic protein on DNA synthesis and cell replication in calvarial and fibroblast cultures (1985) Clin Orthop, 198, pp. 289-294; 
Centrella, M., Casinghino, S., Kim, J., Pham, T., Rosen, V., Wozney, J., McCarthy, T.L., Independent changes in type I and type II receptors for transforming growth factor β induced by bone morphogenetic protein 2 parallel expression of the osteoblast phenotype (1995) Mol Cell Biol, 15, pp. 3273-3281; 
Vukicevic, S., Luyten, F.P., Redi, A.H., Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin (1989) Proc Natl Acad Sci USA, 86, pp. 8793-8797
},
	month = {2001///},
	pages = {306 - 312},
	abstract = {In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 μg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. {\textcopyright} 2001 John Wiley \& Sons, Inc.},
	keywords = {alkaline phosphatase, Animals, article, Biomedical engineering, BMP-2, Bone, bone development, bone marrow, Bone Marrow Cells, Bone morphogenetic proteins, Cell culture, Cell Line, cell proliferation, Cells, concentration response, dexamethasone, differentiation inducing factor, dose response, Dose-Response Relationship, Drug, enzyme activity, fibroblast, Fibroblasts, Glucocorticoids, human, human cell, Humans, male, MRC-5 fibroblasts, Nacre, natural product, osteoblast, Ostreidae, oyster, phenotype, Proteins, Pulmonary diseases, Rats, Rats, Wistar, Solubility, stroma cell, time, Time Factors, Transforming Growth Factor beta, unclassified drug, Water, Water-soluble matrix},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034769134\&partnerID=40\&md5=f0ec664a074b3a67745c806152793d54},
	author = {Almeida, M.J. and Pereira, L. and Milet, C. and Haigle, J. and Barbosa, M. and Lopez, E.}
}
@article {2541,
	title = {Effect of chemical composition on hydrophobicity and zeta potential of plasma sprayed HA/CaO-P                    2O
                    5 glass coatings
},
	journal = {BiomaterialsBiomaterials},
	volume = {22},
	year = {2001},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(01)00059-XPubMed ID: 11603581Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Laborat{\'o}rio de Biomateriais, Inst. de Engenharia Biomedica (INEB), Rua do Campo Alegre 823, 4150 Porto, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: Calcium Compounds; Durapatite, 1306-06-5; lime, 1305-78-8; Oxides; Phosphorus Compounds; phosphorus pentoxide, 1314-56-3; Serum Albumin, BovineReferences: Klein, C.P.A.T., Patka, P., Van der Lubbe, H.B.M., Plasma-sprayed coatings of tetracalciumphosphate, hydroxyl-apatite, and α-TCP on titanium alloy: An interface study (1991) J Biomed Mater Res, 25, pp. 53-65;Jansen, J.A., Van der Waerden, J.P., Wolke, J.G., Histologic investigation of the biologic behavior of different hydroxyapatite plasma-sprayed coatings in rabbits (1993) J Biomed Mater Res, 14, pp. 603-610; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., Comparative biological properties of HA plasma-sprayed coatings having different crystallinities (1994) J Mater Sci: Mater Med, 5, pp. 11-17; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., Comparison of the early production of extracellular matrix on dense hydroxyapatite and hydroxyapatite-coated titanium in cell and organ culture (1995) Biomaterials, 16, pp. 489-495; 
Labat, B., Chanson, A., Frey, J., Effects of γ-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts (1995) J Biomed Mater Res, 29, pp. 1397-1401; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., Adult human bone cells derived from jaw bones cultured on plasma-sprayed or polished surfaces to titanium or hydroxyapatite discs (1996) J Mater Sci: Mater Med, 7, pp. 21-28; 
Silva, P.L., Santos, J.D., Monteiro, F.J., Knowles, J.C., Adhesion and microstructural characterisation of plasma-sprayed hydroxyapatite/glass ceramic coatings onto Ti-6Al-4V substrates (1998) Surf Coating Technol, 102, pp. 191-196; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P 2O 5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced HA plasma-sprayed coatings (1999) J Mater Sci: Mater Med, 10 (9), pp. 567-576; 
Lapin, M., Warocquier-Cl{\'e}rout, R., Legris, C., Degrange, M., Luizard, M.F.S., Correlation between roughness and wettability, cell adhesion and cell migration (1997) J Biomed Mater Res, 36, pp. 99-108; 
Williams, D.F., (1982) Fundamental aspects of biocompatibility, , Boca Raton: CRC Press; 
Adamson, W., (1990) Physical chemistry of surfaces, 5th ed., , New York: Wiley; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions: Structure and properties of the composites (1995) J Mater Sci: Mater Med, 6, pp. 348-352; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass-reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Chen, P., Li, D., Boruvka, L., Rotenberg, Y., Neumann, A.W., Automation of axisymmetric drop shape analysis for measurements of interfacial tensions and contact angles (1990) Coll and Surf, 43, pp. 151-197; 
Cheng, P., Neumann, A.W., Computational evaluation of axisymmetric drop shape analysis-profile (ASA-P) (1992) Coll and Surf, 62, pp. 297-306; 
Young, T., (1835) Miscellaneous works, 1, p. 418. , Peacock G, editor. London: Murray; 
Schrader, M.E., Young Dupr{\'e} revisited (1995) Langmuir, 11, p. 3585; 
Owens, D.K., Wendt, R.D., Estimation of the surface free energy of polymers (1969) J Appl Polym Sci, 13, pp. 1741-1769; 
Williams, R.L., Williams, D.F., Albumin adsorption on metal surfaces (1988) Biomaterials, 9, pp. 206-212; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tension, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Tripp, B.C., Magda, J.J., Andrade, J.D., Adsorption of globular proteins at the air/water interface as measured via dynamic surface tension: Concentration dependence, mass-transfer and adsorption kinetics (1995) J Coll Interface Sci, 173, pp. 16-27; 
Voigt, A., Thiel, O., Williams, D., Policova, Z., Zing, W., Neumann, A.W., Axisymmetric drop shape analysis (ADSA) applied to protein solutions (1991) Coll and Surf, 58, pp. 315-326; 
Suzuki, T., Nishizawa, K., Yokogawa, Y., Kameyama, T., Time-dependent variation of the surface structure of bioceramics in tissue culture medium and the effect of adhesiveness of cells (1996) J Ferment Bioeng, 81, pp. 226-232; 
Susuki, T., Yamamoto, T., Yoriyama, M., Kameyama, T., Surface instability of calcium phosphate ceramics in tissue culture medium and the effect on adhesion and growth of anchorage-dependent animal cells (1997) J Biomed Mater Res, 34, pp. 504-517; 
Haynes, C.A., Norde, W., Globular proteins at solid/liquid interfaces (1994) Coll and Surf B: Biointerfaces, 2, pp. 517-566; 
Kondori, K., Soito, M., Takebe, T., Yasukawa, A., Adsorption of bovine serum albumin on synthetic carbonate calcium hydroxyapatite (1995) J Coll Interface Sci, 184, pp. 124-129
},
	month = {2001///},
	pages = {3105 - 3112},
	abstract = {Multilayered plasma sprayed coatings on the surface of Ti-6Al-4V alloys have been prepared, which were composed of an underlayer of HA and a surface layer of a CaO-P                        2O
                        5 glass-HA composite, with 2 or 4 wt\% of glass. Contact angle and surface tension variation with time, for both water and a protein solution, were determined by the sessile and pendent drop methods respectively using the ADSA-P software. Wettability studies showed that hydrophobicity of the coatings increase with the glass addition. The work of adhesion of albumin was also altered in a controlled manner by the addition of the CaO-P
                        2O
                        5 glass, being lower on the composite coatings than on HA. Zeta potential (ZP) results showed that composite coatings presented a higher net negative charge than HA coatings and that ZP values were also influenced by the content of the glass. This study demonstrated that the surface properties of those coatings may be modified by the addition of CaO-P
                        2O
                        5 glass. {\textcopyright} 2001 Published by Elsevier Science Ltd.
},
	keywords = {Adhesion, alloy, analytic method, article, bovine serum albumin, Calcium Compounds, calcium oxide, chemical composition, Chemical modification, Coatings, Composition, computer program, Contact angle, Durapatite, glass, hydrophobicity, hydroxyapatite, Hydroxyapatite glass composites, oxide, Oxides, pentoxide, Phosphorus Compounds, priority journal, protein, Serum Albumin, Bovine, Sprayed coatings, Surface charge, Surface Properties, Surface tension, Surface tension zeta potential, time, unclassified drug, Water, wettability, Wetting, X-Ray Diffraction, Zeta potential},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034841180\&partnerID=40\&md5=552d64a5e7743f1d3c19fafc42a6e38d},
	author = {Ferraz, M.P. and Monteiro, F.J. and Serro, A.P. and Saramago, B. and Gibson, I.R. and Santos, J.D.}
}
@article {2543,
	title = {HA and double-layer HA-P                    2O
                    5/CaO glass coatings: Influence of chemical composition on human bone marrow cells osteoblastic behavior
},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {12},
	year = {2001},
	note = {Cited By (since 1996): 18Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1011245828046Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Rua Dr. Manuel Pereira da Silva, 4200 Porto, PortugalReferences: Klein, C.P.A.T., Patka, P., Van der Lubbe, H.B.M., (1991) J. Biomed. Mater. Res., 25, p. 53;Jansen, J.A., Van Der Waerden, J.P.C.M., Wolke, J.G.C., (1993) J. Mater. Sci Mater. Med., 4, p. 466; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., (1994) J. Mater. Sci. Mater. Med., 5, p. 11; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., (1995) Biomaterials, 16, p. 489; 
Labat, B., Chanson, A., Frey, J., (1995) J. Biomed. Mater. Res., 29, p. 1397; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., (1996) J. Mater. Sci. Mater. Med., 7, p. 21; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., (1999) J. Mater. Sci: Mater. Med., 10, p. 567; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mat. Res., 45, p. 376; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res., 47, p. 603; 
Anselme, K., Sharrock, P., Hardouin, P., Dard, M., (1997) J. Biomed. Mat. Res., 34, p. 247; 
Ong, J.L., Prince, C.W., Lucas, L.C., (1995) J. Biomed. Mat. Res., 29, p. 165; 
Gregoire, M., Orly, I., Kerebel, L.-M., Kerebel, B., (1987) Biol. Cell., 255, p. 59; 
Morgan, J., Holtman, K., Stanford, C., (1995) J. Dent. Res., 74, p. 190; 
Chou, L., Firth, J.D., Uitto, V.-J., Brunette, D.M., (1995) J. Cell. Sci., 108, p. 1563; 
Chou, L., Marek, B., Wagner, W.R., (1999) Biomaterials, 20, p. 977; 
Susuki, T., Nishizawa, K., Yokogawa, Y., Nagata, F., Kawamoto, Y., Kameyama, T., (1996) J. Ferment. Bioeng., 81, p. 226; 
Lampin, M., Warocquier-Clerout, R., Legris, C., Degrange, M., Sigot-Luizard, M.F., (1997) J. Biomed. Mater. Res., 36, p. 99; 
Best, S., Sim, B., Kayser, M., Downes, S., (1997) J. Mater. Sci. Mater. Med., 8; 
Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., (1999) J. Mater. Sci. Mater. Med., 10, p. 663; 
Maniatopoulos, C., Sodek, J., Melcher, A., (1988) Cell Tissue Res., 254, p. 317; 
Gundle, R., Joyner, C.Y., Triffit, J.T., (1995) Bone, 16, p. 597; 
Fernandes, M.H., Costa, M.A., Carvalho, G.S., (1997) J. Mater. Sci. Mater. Med., 8, p. 61; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., (1997) J. Mater. Sci. Mater. Med., 8, p. 233; 
Fernandes, M.H., (1999) J. Biomat. App., 14, p. 113; 
Martin, T.J., Findlay, D.M., Heath, J.K., Ng, K.W., (1993) Handbook of Experimental Pharmacology, p. 149. , edited by J. R. Mundy and T. J. Martin (Springer-Verlag, Berlin); 
Stein, G.S., Lian, J.B., (1993) Cellular and Molecular Biology of Bone, p. 47. , edited by M. Noda (Academic Press Inc., Tokyo); 
Coelho, M.J., Trigo Cabral, A., Fernandes, M.H., Biomaterials, , in press; 
Coelho, M.J., Fernandes, M.H., Biomaterials, , in press; 
Anderson, H.C., Morris, D.C., (1993) Handbook of Experimental Pharmacology, p. 267. , edited by J. R. Mundy and T. J. Martin (Springer-Verlag, Berlin); 
Krajewski, A., Piancastelli, A., Malavolti, R., (1998) Biomaterials, 19, p. 637; 
Krajewski, A., Malavolti, R., Piancastelli, A., (1996) Biomaterials, 17, p. 53; 
Ducheyne, P., Kim, C.S., Pollack, S.R., (1992) J. Biomed. Mater. Res., 26, p. 147; 
Susuki, T., Yamamoto, T., Toriyama, M., Nishizawa, K., Yokogawa, Y., Mucalo, M.R., Kawamoto, Y., Kameyama, T., (1997) J. Biomed. Mater. Res., 34, p. 507; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Biomaterials, , in press; 
Kokubo, T., (1993) Bone-Bonding Biomaterals, p. 31. , edited by P. Ducheyne, T. Kokubo and C. A. Blitterswijk (Reed Health-Care Communications, Leiden, The Netherlands); 
Daculsi, G., Legeros, R.Z., Henghebaert, M., Barbieux, I., (1990) Calcif. Tissue Int., 46, pp. 20-27; 
Bagambisa, F.B., Joos, U., Schilli, W., (1993) J. Biomed. Mat. Res., 27, pp. 1047-1055; 
De Bruijn, J.D., Van Blitterswijk, C.A., Davies, J.E., (1995) J. Biomed. Mat. Res., 29, pp. 89-99; 
Gwynn, I.A.P., (1994) J. Mater. Sci. Mater Med., 5, pp. 357-360; 
Ghannam, E.L., Ducheyne, P., Spapiro, I., (1995) J. Biomed Mat. Res., 29, pp. 359-370; 
Kirkpatrick, C.J., Wagner, M., Kohler, H., Bittinger, F., Otto, M., Klein, C.L., (1997) J. Mater. Sci. Mater Med., 8, pp. 131-141; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., J. Biomed. Mater. Res., , in press
},
	month = {2001///},
	pages = {629 - 638},
	abstract = {Human osteoblastic bone marrow derived cells were cultured for 28 days onto the surface of a glass reinforced hydroxyapatite (HA) composite and a commercial type HA plasma sprayed coatings, both in the "as-received" condition and after an immersion treatment with culture medium during 21 days. Cell proliferation and differentiation were analyzed as a function of the chemical composition of the coatings and the immersion treatment. Cell attachment, growth and differentiation of osteoblastic bone marrow cells seeded onto "as-received" plasma sprayed coatings were strongly affected by the time-dependent variation of the surface structure occurring during the first hours of culture. Initial interactions leading to higher amounts of adsorbed protein and zeta potential shifts towards negative charges appeared to result in surface structures with better biological performance. Cultures grown onto the pretreated coatings showed higher rate of cell proliferation and increased functional activity, as compared to those grown onto the corresponding "as-received" materials. However, the cell behavior was similar in the glass composite and HA coatings. The results showed that the glass composites present better characteristics for bone cell growth and function than HA. In addition, this work also provide evidence that the biological performance of the glass composites can be modulated and improved by manipulations in the chemical composition, namely in the content of glass added to HA. {\textcopyright} 2001 Kluwer Academic Publishers.},
	keywords = {Adsorption, alkaline phosphatase, article, Biomedical engineering, Bone, bone marrow, bone marrow cell, Calcium, calcium oxide, cell adhesion, Cell culture, cell differentiation, cell growth, cell proliferation, chemical composition, Coatings, controlled study, culture medium, enzyme activity, glass, Growth kinetics, human, human cell, hydroxyapatite, immersion, osteoblast, oxygen, performance, phosphorus, priority journal, protein binding, protein content, Proteins, Scanning electron microscopy, Surface charge, surface property, time, X ray diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034918167\&partnerID=40\&md5=3783f798f6eea84dfff83e16a393fe54},
	author = {Ferraz, M.P. and Fernandes, M.H. and Santos, J.D. and Monteiro, F.J.}
}
@article {2548,
	title = {Histological studies of double layer HA/CaO-P                    2O
                    5 glass plasma sprayed coatings using Rabbit model
},
	journal = {Key Engineering MaterialsKey Eng Mat},
	volume = {192-195},
	year = {2001},
	note = {Conference code: 57760Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; Instituto de Engenharia Biomedica, (INEB), Porto, PortugalReferences: Lacefield, W.R., (1988) Bioceramics: Material Characteristics Versus In Vivo Behaviour, pp. 72-80. , P. Ducheyne, J.E. Lemons. New York: New York Academy of Science Publishers;Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., (1994) J Biomed Mater Res, 28, pp. 961-967; 
Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., (1994) J Biomed Mater Res, 28, pp. 909-917; 
Burnie, J., Gilchrist, T., (1983) Ceramic in Surgery, pp. 169-176. , P. Vicenzini. Amsterdam: Elsevier Science Publishers; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabrai, A., Santos, J.D., Monteiro, F.J., (1999) J Mater Sci Mater Med, 10 (9), pp. 567-576; 
Oonishi, H., Hench, L.L., Wilson, J., Sugihara, F., Tsuji, E., Kushitani, S., Iwak, H., (1999) J Biomed Mater Res, 44, pp. 31-43; 
Kitsugi, T., Nakamura, T., Oka, M., Senaha, Y., Got, T., (1996) J Biomed Mater Res, 30, pp. 261-269; 
Klein, C.P.A.T., Patka, P., Van Der Lubbe, H.B.M., Wolke, J.G.V., De Groot, K., (1991) J Biomed Mater Res, 25, pp. 53-65
},
	month = {2001///},
	pages = {449 - 452},
	publisher = {Trans Tech Publ},
	address = {Bologna, Italy},
	abstract = {The in vivo biological performance of a double layer HA/CaO-P                        2O
                        5 glass and HA plasma sprayed coatings was assessed, using cylindrical implants that were placed in rabbits tibiae and histologically characterised after 2, 4, 8 and 16 weeks of implantation. For both types of coatings, long-term histological results indicated successful contact between implanted materials and bone, with organised new bone established at the interface. The main difference between the two coatings corresponded to the shorter implantation periods, where the composite coating induced faster osteointegration than HA coating. The faster formation of organised bone for composite coatings is discussed in terms of the presence of phase composition in the coatings structure.
},
	keywords = {Bond strength (materials), Bone, Double layer coatings, hydroxyapatite, Implants (surgical), In vivo evaluation, Interfaces (materials), morphology, Phase composition, Phosphates, Plasma sprayed coatings, Rabbit model, Scanning electron microscopy, Sprayed coatings, X ray diffraction analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035147691\&partnerID=40\&md5=1857cde1f297ab045f2369691bdbdc8c},
	author = {Ferraz, M.P. and Santos, J.D. and Afonso, A. and Vasconcelos, M. and Monteiro, F.J.}
}
@article {2545,
	title = {Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {54},
	year = {2001},
	note = {Cited By (since 1996): 40Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/1097-4636(20010315)54:4<463::AID-JBM10>3.0.CO;2-YPubMed ID: 11426590Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Laborat{\'o}rio de Biomateriais, INEB, Rua do Campo Alegre, 823, 4150-180 Porto, PortugalChemicals/CAS: Bone Substitutes; Composite Resins; Durapatite, 1306-06-5References: Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Nakamura, T., Kitsugi, T., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J Biomed Mater Res, 24, pp. 471-488;Klein, C.P.A.T., Driessen, A.A., De Groot, K., Van der Hooff, A., Biodegradation behavior of various calcium phosphate materials in bone tissue (1983) J Biomed Mater Res, 17, pp. 769-784; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural Characterization of Glass Reinforced Hydroxyapatite Composites (1994) Biomaterials, 15, pp. 5-10; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass Reinforced Hydroxyapatite: A Comprehensive Study of the Effect of Glass Composition on the Crystallography of the Composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Glass Reinforced Hydroxyapatite Composites: Secondary Phases Proportions and Densification Effects on Biaxial Bending Strength (1999) J Biomed Mater Res, 48, pp. 734-740; 
Lopes, M.A., Saramago, B., Santos, J.D., Monteiro, F.J., Hydrophobicity, Surface Tension and Zeta Potential of Glass Reinforced Hydroxyapatite Composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow Cytometry for Assessing Biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., CaP-rich layer formed on high-strength bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 331-343; 
Cho, S.B., Nakanishi, K., Kokubo, T., Soga, N., Ohtsuki, C., Nakamura, T., Kitsugi, T., Yamamuro, T., Dependence of Apatite Formation on Silica Gel on Its Structure: Effect of Heat Treatment (1975) J Am Ceram Soc, 78, pp. 1769-1774; 
Neo, M., Kotani, S., Fujita, Y., Nakamura, T., Yamamuro, T., Bando, Y., Ohtsuki, C., Kokubo, T., Differences in ceramic-bone interface between surface-active ceramics and resorbable ceramics: A study by scanning and transmission electron microscopy (1992) J Biomed Mater Res, 26, pp. 255-267; 
Hamson, K.R., Toth, J.M., Stiehl, J.B., Lynch, K.L., Preliminary experience with a novel model assessing in vivo mechanical strength of bone grafts and substitute materials (1995) Calcif Tissue Int, 57, pp. 64-68; 
Hing, K.A., (1996) Assessment of porous hydroxyapatite for bone replacement, , London: University of London; 
Albrektsson, T., Johansson, C., Quantitative bone tissue reactions to various metallic materials with reference to the so-called osseointegration concept (1991), pp. 357-363. , Davies JE, editor. The bonebiomaterial interface. Toronto: University of Toronto PressProbster, L., Voigh, C.H., Fuhrman, G., Gross, U.M., Tensile and torsional shear strength of the bone implant interface of titanium implants in the rabbit (1994) J Mater Sci: Mater Med, 5, pp. 314-319; 
Kokubo, T., Kushitani, H., Ebisawa, Y., Kitsugi, T., Kotani, S., Oura, K., Yamamuro, T., Apatite formation on bioactive ceramics in body environment (1989), 1, pp. 157-162. , Oonishi H, Aoki H, Sawai K, editors. Bioceramics. Tokyo: Ishiyaku EuroAmerica, IncLeGeros, R.Z., (1991), pp. 4-45. , Meyers HM, editor. Calcium phosphates in oral biology and medicine. New York: KargerOhtsuki, C., Kokubo, T., Neo, M., Kotani, S., Yamamuro, T., Nakamura, T., Bando, Y., Bone-Bonding Mechanism of Sintered β-3CaO-P 2O 5 (1991) Phosphorus Research Bulletin, 1, pp. 191-196; 
Kotani, S., Fujita, Y., Kitsugi, T., Nakamura, T., Yamamuro, T., Ohtsuki, C., Kokubo, T., Bone bonding mechanism of β-tricalcium phosphate (1991) J Biomed Mater Res, 25, pp. 1303-1315; 
Lu, J.X., Gallur, A., Flautre, B., Anselme, K., Descamps, M., Thierry, B., Hardouin, P., Comparative study of tissue reactions to calcium phosphate ceramics among cancellous, cortical, and medullar bone site in rabbits (1998) J Biomed Mater Res, 42, pp. 357-367; 
Kitsugi, T., Nakamura, T., Oka, M., Senaha, Y., Goto, T., Bonebonding behavior of plasma-sprayed coatings of bioglass, AW-glass ceramic, and tricalcium phosphate on titanium alloy (1996) J Biomed Mater Res, 30, pp. 261-269; 
Kitsugi, T., Yamamuro, T., Nakamura, T., Kotani, S., Kokubo, T., Takeuchi, H., Four calcium phosphate ceramics as bone substitutes for non-weight-bearing (1993) Biomaterials, 14, pp. 216-224; 
De Lauge, G.L., De Putter, C., De Wijs, F.L.J.A., Histological and ultrastructural appearance of the hydroxy apatite-bone interface (1990) J Biomed Mater Res, 24, pp. 829-845; 
Yamada, S., Nakamura, T., Kokubo, T., Oka, M., Yamamuro, T., Degradation of the apatite layer formed on bioactive ceramics and the underlying ceramic surface by osteoclasts in a culture system (1994) Cells and Materials, 4, pp. 347-356; 
Brunski, J.B., Influence of biomechanical factors at the bone-biomaterial interface (1990), pp. 391-405. , Davies JE, editor. The bone-biomaterial interface. Toronto: University of Toronto PressDhert, J.A., Thomsen, P., Blomgren, A.K., Esposito, M., Ericson, L.E., Verbout, A.J., Integration of press-fit implants in cortical bone: A study on interface kInetics (1998) J Biomed Mater Res, 41, pp. 574-583; 
Gao, T.J., Lindholm, T.S., Kommonen, B., Ragni, P., Paronzini, A., Lindholm, T.C., Microscopic evaluation of bone-implant contact beween HA, bioactive glass and tricalcium phosphate implanted in sheep diaphyseal defects (1995) Biomaterials, 16, pp. 1175-1179; 
Oonishi, H., Hench, L.L., Wilson, J., Sugihara, F., Tsuji, E., Kushitani, S., Iwaki, H., Comparative bone growth behavior in granules of bioceramic materials of various sizes (1999) J Biomed Mater Res, 44, pp. 31-43; 
Marie, P.J., De Vernejoul, M.C., Lomri, A., Stimulation of bone formation in osteoporosis patients treated with fluoride associated with increased DNA synthesis by osteoblast cells in vitro (1992) J Bone Miner Res, 7, pp. 103-113; 
Rodan, G.A., Introduction to bone biology (1992) Bone, 13, pp. S3-S6
},
	month = {2001///},
	pages = {463 - 469},
	publisher = {John Wiley \& Sons Inc},
	abstract = {In vitro and in vivo bioactivity studies were performed to assess the biocompatibility of CaO-P                        2O
                        5 glass-reinforced hydroxyapatite (GR-HA) composites. The ability to form an apatite layer by soaking in simulated body fluid (SBF) was examined and surfaces were characterized using FTIR reflection and thin-film X-ray diffraction analyses. Qualitative histology, histomorphometric measurements, and push-out testing were performed in a rabbit model for characterizing bone/implant bonding. Under the in vitro conditions using SBF, an apatite layer could not be formed on GR-HA composites within 8 weeks. Results of push-out testing showed bonding between the composites and bone, ranging from 130-145 N after 2 weeks of implantation. After the longest implantation period, 16 weeks, the GR-HA composite prepared with the higher content of CaO-P
                        2O
                        5 glass showed the highest bonding force, 606 {\textpm} 45 N, compared to 459 {\textpm} 30 N for sintered HA. Development of immature bone and modifications in the turnover of a more mature bone on the surface of GR-HA composites were similar to those on sintered HA. {\textcopyright} 2000 John Wiley \&amp; Sons, Inc.
In vitro and in vivo bioactivity studies were performed to assess the biocompatibility of CaO-P
                        2O
                        5 glass-reinforced hydroxyapatite (GR-HA) composites. The ability to form an apatite layer by soaking in simulated body fluid (SBF) was examined and surfaces were characterized using FTIR reflection and thin-film X-ray diffraction analyses. Qualitative histology, histomorphometric measurements, and push-out testing were performed in a rabbit model for characterizing bone/implant bonding. Under the in vitro conditions using SBF, an apatite layer could not be formed on GR-HA composites within 8 weeks. Results of push-out testing showed bonding between the composites and bone, ranging from 130-145 N after 2 weeks of implantation. After the longest implantation period, 16 weeks, the GR-HA composite prepared with the higher content of CaO-P
                        2O
                        5 glass showed the highest bonding force, 606 {\textpm} 45 N, compared to 459 {\textpm} 30 N for sintered HA. Development of immature bone and modifications in the turnover of a more mature bone on the surface of GR-HA composites were similar to those on sintered HA. {\textcopyright} 2000 John Wiley \&amp; Sons, Inc.
},
	keywords = {animal experiment, animal tissue, Animals, article, Biocompatibility, Body fluids, Bonding, Bone, Bone Substitutes, bone turnover, Ceramic materials, Composite Resins, Durapatite, Fiber reinforced materials, Fourier transform infrared spectroscopy, glass, Glass reinforced hydroxyapatite composites, Glass-reinforced hydroxyapatite composites, Histological studies, histology, Histomorphometric measurements, hydroxyapatite, implant, implantation, Implants (surgical), In vitro bioactivity, In vivo evaluation, Interfaces (materials), male, nonhuman, orthopedic equipment, ossification, Prosthesis Implantation, Push out testing, Push-out testing, rabbit, Rabbits, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, Surgical equipment, tibia, Time Factors, X ray diffraction, X ray diffraction analysis},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035869784\&partnerID=40\&md5=9464ca5ca74972a99d8092171252e0b3},
	author = {Lopes, M.A. and Santos, J.D. and Monteiro, F.J. and Ohtsuki, C. and Osaka, A. and Kaneko, S. and Inoue, H.}
}
@article {2563,
	title = {Classification of breast tissue by electrical impedance spectroscopy},
	journal = {Medical and Biological Engineering and ComputingMed. Biol. Eng. Comput.},
	volume = {38},
	year = {2000},
	note = {Cited By (since 1996): 42Export Date: 21 November 2012Source: ScopusCODEN: MBECDPubMed ID: 10829386Language of Original Document: EnglishCorrespondence Address: Marques de Sa, J.P.; INEB, Facul. Engenharia Universidade Porto, Porto, Portugal; email: jmsa@fe.op.ptReferences: Brown, B.H., Barber, D.C., Morice, A.H., Leathard, A.D., Cardiac and respiratory related electrical impedance changes in the human thorax (1994) IEEE Trans. Biomed. Eng., BME-41, pp. 729-734;Campbell, A.M., Land, D.V., Dielectric properties of female breast tissue measured in vitro at 3.2 GHz (1992) Phys. Med. Biol., 37, pp. 193-210; 
Duda, R., Hart, P., (1973) Pattern Classification and Scene Analysis, , Wiley, New York; 
Foley, D.H., Considerations of sample and feature size (1972) IEEE Trans. Inform. Theory, IT-18, pp. 618-626; 
Foster, K.R., Schwan, H.P., Dielectric properties of tissues and biological materials: A critical review (1989) CRC Crit. Reviews Biomed. Eng., 17, pp. 25-104; 
Fukunaga, K., (1972) Introduction to Statistical Pattern Recognition, , Academic Press, New York and London; 
Gersing, E., Sch{\"a}fer, M., Osypka, M., The appearance of positive phase angles in impedance measurements on extended biological subjects (1995) Innov. Techn. Biol. Med., 16 (SPEC. ISSUE 2), pp. 72-76. , Proc. {\textquoteright}CAIT Workshop on Electrode-Skin Interface in EIT{\textquoteright}; 
Heinitz, J., Minet, O., Dielectric properties of female breast tumors (1995) Proc. {\textquoteright}9th Int. Conf. Electrical Bioimpedance{\textquoteright}, pp. 356-359. , University of Heidelberg Press; 
Henderson, R.P., Webster, J.G., An impedance camera for spatially specific measurements of the thorax (1978) IEEE Trans. Biomed. Eng., BME-27, pp. 250-254; 
Jossinet, J., Variability of impedivity in normal and pathological breast tissue (1996) Med. Biol. Eng. Comput., 34, pp. 346-350; 
Jossinet, J., The impedivity of freshly excised human breast tissue (1998) Physiol. Meas., 19, pp. 61-75; 
Jossinet, J., Lavandier, B., The discrimination of excised cancerous breast tissue samples using impedance spectroscopy (1998) Bioelectrochem. Bioenerg., 45, pp. 161-167; 
Kubicek, W.G., Patterson, R.P., Witsoe, D.A., Impedance cardiography as a noninvasive method of monitoring cardiac function and other parameters of the cardiovascular system (1970) Ann. N.Y. Acad. Sciences, 170, pp. 24-732; 
Morimoto, T., Kinouchi, Y., Iritani, T., Kimura, S., Konishi, Y., Mitsuyama, N., Komaki, K., Monden, Y., Measurement of the electrical bio-impedance of breast tumors (1990) Eur. Surg. Res., 22, pp. 86-92; 
Raudys, S., Pikelis, V., On dimensionality, sample size, classification error and complexity of classification algorithm in pattern recognition (1980) IEEE Trans. Patt. Anal. Mach. Intel., PAMI-2, pp. 242-252; 
Shapiro, S.S., Wilk, S.S., Chen, S.W., A comparative study of various tests for normality (1968) J. Am. Stat. Ass., 63, pp. 1343-1372; 
Swain, P.H., The decision tree classifier: Design and potential (1977) IEEE Trans. Geosci. Electronics, GE-15, pp. 142-147; 
Schwan, H.P., Alternating current spectroscopy of biological substances (1959) Proc. IRE, 47, pp. 1841-1855; 
Surowiec, A.J., Stuchly, S.S., Barr, J.R., Swarup, A., Dielectric properties of breast carcinoma and the surrounding tissues (1988) IEEE Trans. Biomed. Eng., BME-35, pp. 257-263; 
Tachibana, S., Aguilar, J.A., Birzis, L., Scanning the interior of living brain by impedography (1970) J. of App. Physiol., 28, pp. 534-539
},
	month = {2000///},
	pages = {26 - 30},
	publisher = {Peter Peregrinus Ltd},
	abstract = {Electrical impedance spectroscopy is a minimally invasive technique that has clear advantages for living tissue characterisation owing to its low cost and ease of use. The present paper describes how this technique can be applied to breast tissue classification and breast cancer detection. Statistical analysis is used to derive a set of rules based on features extracted from the graphical representation of electrical impedance spectra. These rules are used hierarchically to discriminate several classes of breast tissue. Results of statistical classification obtained from a data set of 106 cases representing six classes of excised breast tissue show an overall classification efficiency of ~92\% with carcinoma discrimination > 86\%.Electrical impedance spectroscopy is a minimally invasive technique that has clear advantages for living tissue characterization owing to its low cost and ease of use. The present paper describes how this technique can be applied to breast tissue classification and breast cancer detection. Statistical analysis is used to derive a set of rules based on features extracted from the graphical representation of electrical impedance spectra. These rules are used hierarchically to discriminate several classes of breast tissue. Results of statistical classification obtained from a data set of 106 cases representing six classes of excised breast tissue show an overall classification efficiency of approximately 92\% with carcinoma discrimination >86\%.
},
	keywords = {article, Breast, breast cancer, Breast Neoplasms, cancer classification, Data classification, Discriminant Analysis, Electric Impedance, Electrical impedance spectroscopy, Electrodiagnosis, Feature extraction, Female, human, Humans, impedance, Impedance spectroscopy, major clinical study, Medical imaging, Signal Processing, Computer-Assisted, Spectroscopy, Statistical analysis, Statistical methods, Tissue, Tissue characterisation},
	isbn = {01400118 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033962139\&partnerID=40\&md5=1510d493688d338d985aad2a31d1caeb},
	author = {Estrela Da Silva, J. and Marques De S{\'a}, J.P. and Jossinet, J.}
}
@conference {2555,
	title = {A computational model of visual cortex receptive fields using spatio-temporal filters},
	booktitle = {Proceedings of the IEEE/EMBS Region 8 International Conference on Information Technology Applications in Biomedicine, ITABProc IEEE EMBS Reg 8 Int Conf Inf Technol Appl Biomed ITAB},
	series = {ITAB-ITIS 2000},
	year = {2000},
	note = {Conference code: 58015Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Milanova, M.G.; INEB, Inst. of Biomedical Engineering, University of Porto, Pra{\c c}a Coronel Pacheco, 1 4050 Porto, Portugal; email: milanova@ineb.fe.up.ptReferences: Hubel, D.H., Wiesel, T.N., Receptive fields, binocular interaction, and functional architecture in the cat{\textquoteright}s visual cortex (1962) Journal of Physiology (London), 160, pp. 106-154;DeAngelis, G.C., Ohzawa, I., Freeman, R.D., Receptive-field dynamic in the central visual pathways (1995) Trends in Neuroscience, 18, pp. 451-458; 
Atick, J., Redlich, N., What does the retina know about natural scenes? (1992) Neural Computation, 4, pp. 196-210; 
Barlow, H.B., What is the computational goal of the neocortex? (1994) Large-Scale Neuronal Theories of the Brain, pp. 1-22. , C. Koch and J. L. Davis, eds, Cambridge, MA, MIT Press; 
Olshausen, B.A., Field, D.J., Emergence of simple-cell receptive field properties by learning a sparse code for natural images (1996) Nature, 381, pp. 607-609; 
Rao, R.P.N., Ballard, D.H., Dynamic model of visual recognition predicts neural response properties in the visual cortex (1997) Neural Computation, 9, pp. 721-763; 
Olshausen, B.A., (2000) Sparse coding of time-varying natural images, , http://psyweb.ucdavis.edu/olshausen; 
Oja, E.E., Neural networks, principal components and subspaces (1989) International Journal of Neural Systems, 1, pp. 61-68; 
Field, D.J., What is the goal of sensory coding? (1994) Neural Computation, 6, pp. 559-601; 
Field, D.J., Relations between the statistics of natural images and the response properties of cortical cells (1987) J. Opt. Soc. Am.A, 4, pp. 2379-2394; 
Bell, A.J., Sejnowski, T.J., The independent components of natural scenes are edge filters (1997) Vision Research, 37, pp. 3327-3338; 
Smith, A.T., Snowdon, R.S., (1994) Visual Detection of Motion, , Academic Press Inc., San Diego; 
Zemel, R.S., Sejnowski, T.J., A model for encoding multiple object motions and self-motion in area MST of primate visual cortex (1998) Journal of Neuroscience, 18, pp. 531-547; 
Van Hateren, J.H., Ruderman, D.L., Independent component analysis of natural image sequences yields spatio-temporal filters similar to simple cells in primary visual cortex (1998) Proc. Royal Soc. London B, 265, pp. 2315-2320; 
Jones, J.P., Palmer, L.A., The two-dimensional spatial structure of simple receptive fields in cat striate cortex (1987) Journal of Neurophysiology, 58, pp. 1187-1211; 
Jones, J.P., Palmer, L.A., An Evaluation of the two-dimensional Gabor filter model of simple receptive fields in cat striate cortex (1987) Journal of Neurophysiology, 58, pp. 1233-1258; 
Jones, J.P., Stepnoski, A., Palmer, L.A., The two-dimensional spectral structure of simple receptive fields in cat striate cortex (1987) Journal of Neurophysiology, 58, pp. 1212-1232; 
Lewicki, M.S., Olshausen, B.A., Probabilistic framework for the adaptation and comparison of image codes (1999) Journal of the Optical Society of America, 16, pp. 1587-1601; 
Chua, L.O., Yang, L.L., Cellular Neural Networks: Theory and Applications (1988) IEEE Trans. on Circuits and Systems, 35, pp. 1257-1290
Sponsors: IEEE},
	month = {2000///},
	pages = {129 - 134},
	address = {Arlington, VA},
	abstract = {It is known that neurons in the primary visual cortex are characterized by receptive fields that are selective for specific spatial orientations. It is believed that the sense of object motion and velocity are also related to these fields, as objects in natural scenes are represented by a sparse set of statistically independent components, such as edges. Currently, computational models of receptive fields consider only spatial components, and thus cannot account for time-varying sensory stimuli. In this paper, a model based on independent components analysis and cellular neural networks is proposed. This approach extends existing models to incorporate temporal aspects of sequences of images of natural scenes.},
	keywords = {Cellular neural networks, Computational methods, ICA, Independent component analysis, Mathematical models, Neurology, Receptive fields, Receptive fields (RF), Spatial filters, Spatio-temporal filters, Visual modeling},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034451070\&partnerID=40\&md5=f1377b4b2f2403be91a21d0feb639fb7},
	author = {Milanova, M.G. and Elmaghraby, A.S. and Wachowiak, M.P. and Campilho, A.}
}
@article {2553,
	title = {Concept, design and fabrication of smart orthopedic implants},
	journal = {Medical Engineering and PhysicsMed. Eng. Phys.},
	volume = {22},
	year = {2000},
	note = {Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: MEPHEdoi: 10.1016/S1350-4533(00)00062-XPubMed ID: 11165144Language of Original Document: EnglishCorrespondence Address: Burny, F.; Service d{\textquoteright}Orthopedie-Traumatologie, Cliniques Universitaires Bruxelles, H{\^o}pital Erasme, Brussels, Belgium; email: fburny@ulb.ac.beChemicals/CAS: Biocompatible Materials; Ceramics; Metals; Stainless Steel, 12597-68-1References: Burny, F., Donkerwolcke, M., Moulart, F., Clinical application of the monitoring of orthopedic implants (1993), pp. 138-146. , Burny R, Puers F, editors. Monitoring of orthopedic implants. A biomaterials microelectronics - challenge. Amsterdam: ElsevierBurny, F., Zucman, J., Bourgois, R., Aubriot, J.H., Sedel, J., Utilisation des jauges extensom{\'e}triques pour la mesure de la consolidation des fractures du tibia trait{\'e}es par enclouage centrom{\'e}dullaire. Etude th{\'e}orique et premiers r{\'e}sultats (1971) Acta Orthop Belg, 37, pp. 266-277;Moulart, F., (1975) R{\'e}{\'e}ducation des fractures hautes du f{\'e}mur. Mesure des d{\'e}formations du mat{\'e}riel d{\textquoteright}ost{\'e}osynthese, , M{\'e}moire de Licence en Kin{\'e}sith{\'e}rapie, Universit{\'e} Libre de Bruxelles; 
Burny, F., Moulart, F., Hinsenkamp, M., Traitement des fractures m{\'e}tastatiques de la region sous-trochant{\'e}rienne. Etude biom{\'e}canique; cas cliniques (1976) Acta Orthop Belg, 42, pp. 31-41; 
Burny, F., Donkerwolcke, M., Telemetry in the musculoskeletal system (1991), pp. 391-394. , Payne PA, editor. Concise encyclopedia of biological and biomedical measurement systems. Oxford: Pergamon PressPuers, R., Burny, F., Biotelemetry in orthopaedics (1992), 3, pp. 285-306. , Muster D. et al., editors. European Materials Research Society Monographs. Biomaterials. Hard Tissue Repair and Replacement, Amsterdam: ElsevierKadefors, R., Kaiser, E., Petersen, I., Energizing implantable transmitters by means of coupled inductance coils (1969) IEEE Trans Biomed Eng, 16 BME, pp. 177-183; 
Ko, W.H., Liang, S.P., Fung, C.D.F., Design of radio-frequency powered coils for implant intruments (1977) Med Biol Eng Comput, 15, pp. 634-640; 
Donaldson, N., De, N., Perkins, T.A., Analysis of resonant coupled coils in the design of radio frequency trancutaneous links (1983) Med Biol Eng Comput, 21, pp. 612-627; 
Grossman, D.G., Machining a machinable glass-ceramic (1978) Vacuum, 28, pp. 55-61; 
Van Schuylenbergh, K., Puers, R., The influence of moisture leakage on implant strain measurements with resistive strain gauges (1993), pp. 125-137. , Burny R, Puers F, editors. Monitoring of orthopedic implants. A biomaterials microelectronics - challenge. Amsterdam: ElsevierWrangl{\'e}n, G., (1985) An introduction to corrosion and protection of metals, , London: Chapman \& Hall; 
Barbosa, M.A., Surface layers and the reactivity of metallic implants (1992), pp. 257-284. , Muster D, editor. Biomaterials - hard tissue repair and replacement, E-MRS Monographs. North-Holland: Elsevier ScienceNicholas, M.G., Active metal brazing (1986) British Ceramic Transactions Journal, 85, pp. 144-146; 
Pattee, H.E., Joining ceramics to metals and other materials (1972) Welding Research Council Bulletin, 178, pp. 2435-2442. , New York; 
Nicholas, M.G., Crispin, R.M., Brazing ceramics with alloys containing titanium (1989) Ceramic Engineering and Science Proceedings, 10, pp. 1602-1612; 
Rodes, F., Zardini, C., Devall, Y., Puers, R., Van Schuylenbergh, K., Burny, F., Donkerwolcke, M., Moulart, F., Direct digitization of low level transducer outputs for low power intracorporal telemetry systems (1993), pp. 401-409. , Mancini P, Fioretti S, editors. 12th Int. Symposium on Biotelemetry, Ancona. Pisa, Italy: Universitaria Litografia FeliciFlack, F.C., James, E.D., Schlapp, D.M., Mutual inductance of air-cored coils: Effect on design of radio-frequency coupled implants (1971) Med Biol Eng Comput, 9, pp. 79-85; 
},
	month = {2000///},
	pages = {469 - 479},
	abstract = {Extensive clinical experience has been built up using orthopaedic implants instrumented with strain gauges connected to a Wheatstone bridge by means of percutaneous leads. This research showed the medical relevance of the monitoring of the deformation of implants as a powerful tool to evaluate nursing and rehabilitation exercises, for tracing dangerous overloads and anticipating implant failure and also to observe the healing process. The IMPACT 3500 project focuses on the instrumentation of femoral implants with on board sensors: regular Benoist-Girard implants have been modified, to contain a {\textquoteright}sensing cell{\textquoteright}, and thoroughly tested in vitro and in vivo. The implant deformations are measured with resistive strain gauges, and the signal is transferred to a personal computer for processing and display, via a hard wired connection, or via a telemetry system. Two fully implantable wireless designs, called Linkstrain and Sealstrain, are powered from the outside by magnetic induction. As Sealstrain contains the whole telemetric system in its cavity, the highest miniaturization was required; this seriously deteriorates the efficiency of the inductive power link. {\textcopyright} 2001 IPEM.},
	keywords = {article, atomic absorption spectrometry, Biocompatible Materials, biomechanics, body movement, Bone Plates, ceramics, compression, Corrosion, Failure of implants, Femoral Fractures, femur, Fracture healing, healing, Humans, materials testing, metal, metal implantation, Metals, microcomputer, Microscopy, Electron, Scanning, Monitoring, Physiologic, Orthopedic implants, orthopedic surgery, priority journal, Prostheses and Implants, Prosthesis Design, prosthesis failure, Rehabilitation, Scanning electron microscopy, sensor, Sensors, Smart implants, Spectrum Analysis, Stainless steel, strain gauge transducer, Strain measurements, Stress, Mechanical, Telemetry, Weight-Bearing},
	isbn = {13504533 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-20244372369\&partnerID=40\&md5=456fea60c077d9bd4569169438349a07},
	author = {Burny, F. and Donkerwolcke, M. and Moulart, F. and Bourgois, R. and Puers, R. and Van Schuylenbergh, K. and Barbosa, M. and Paiva, O. and Rodes, F. and B{\'e}gueret, J.B. and Lawes, P.}
}
@article {2558,
	title = {Direct and indirect effects of P                    2O
                    5 glass reinforced-hydroxyapatite composites on the growth and function of osteoblast-like cells
},
	journal = {BiomaterialsBiomaterials},
	volume = {21},
	year = {2000},
	note = {Cited By (since 1996): 18Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(00)00007-7PubMed ID: 10817269Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University College London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United Kingdom; email: iolsen@eastman.ucl.ac.ukChemicals/CAS: Antigens; Biocompatible Materials; DNA, 9007-49-2; Hydroxyapatites; PhosphatesReferences: Kirkpatrick, C.J., A critical view of current and proposed methodologies for biocompatibility testing: Cytoxicity in vitro (1992) Regulatory Affairs, 4, pp. 13-32;Hanson, S., Lalor, P.A., Niemi, S.M., Ratner, B.D., (1996) Biomaterials Science. An Introduction to Materials in Medicine, pp. 215-242. , B.D. Ratner, \& A.S. Hoffman. Basel: Karger; 
Bou-Gharios, G., Osman, J., Atherton, A., Monoghan, P., Vancheeswaran, R., Black, C., Olsen, I., Expression of ectopeptidaes in scleroderma (1995) Ann Rheum Dis, 54, pp. 111-116; 
Goseki, T., Shimizu, N., Iwasawa, T., Takiguchi, H., Abiko, Y., Effects of in vitro cellular aging on alkaline phosphatase, cathepsin activities and collagen secretion of human periodontal ligament-derived cells (1996) Mech Age Dev, 91, pp. 1761-1783; 
Parkar, M.H., Kuru, L., O{\textquoteright}Hare, M., Newman, H.N., Hughes, F., Olsen, I., Retroviral transduction of periodontal cells with a temperature-sensitive SV40 large T antigen (1999) Archs Oral Biol, 44, pp. 823-834; 
Simon, L.V., Beauchamp, J.R., O{\textquoteright}Hare, M., Olsen, I., Establishment of long-term myogenic cultures from patients with Duchenne muscular dystrophy by retroviral transduction of a temperature-sensitive SV40 large T-antigen construct (1996) Exp Cell Res, 224, pp. 264-271; 
Clover, J., Gowen, M., Are MG-63 and HOS TE 85 human osteosarcoma cell lines representative models of the osteoblastic phenotype (1994) Bone, 15, pp. 585-591; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterisation of glass reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Di Silvio, L., A novel application of two biomaterial for the delivery of growth hormone and its effect on osteoblasts (1995) PhD Thesis, , Univ Lon: Univ Coll Lon Med Sch; 
Kapuscinski, J., Skoczylas, B., Simple and rapid fluorimetric method for DNA microassay (1977) Anal Biochem, 83, pp. 252-257; 
Nishizawa, K., Toriyama, M., Suzuki, T., Kawamoto, Y., Yokogawa, Y., Nagae, H., Effects of the surface wettability and zeta potential of bioceramics on the adhesiveness of anchorage-dependent animal cells (1993) J Ferm Bioengng, 75, pp. 435-437; 
Chesmel, K.D., Clark, C.C., Brighton, C.T., Black, J., Cellular response to chemical and morphologic aspects of biomaterial surfaces. II. The biosynthetic and migratory response of bone cell populations (1995) J Biomed Mater Res, 29, pp. 1101-1110; 
Harmand, M.F., Human cell culture and characterization of cell-biomaterial interface (1992) Clinical Materials, 11, pp. 145-150; 
Lincks, J., Boyan, B.D., Blanchard, C.R., Lohmann, C.H., Liu, Y., Cochran, D.L., Dean, D.D., Schawrtz, Z., Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition (1998) Biomaterials, 19, pp. 2219-2232; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Glass reinforced hydroxyapatite composites: Secondary phases proportions and densification effects on biaxial bending strength (1999) J Biomed Mater Res, 48, pp. 734-740; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Lopes, M.A., Saramago, B., Santos, J.D., Monteiro, F.J., Hydrophobicity and zeta potencial of glass reinforced hydroxyapatite composites (1999) J Biomed Mater Res: Appl Biomater, 45, pp. 370-375; 
Hall, B.K., (1992) Fracture Repair and Regeneration, pp. 33-66. , Boca Raton, FL, USA: CRC Press; 
Legeros, R.Z., (1991) Calcium Phosphates in Oral Biology and Medicine, , Basel: Karger; 
Altankov, G., Groth, T., Fibronectin matrix formation and the biocompatibility of materials (1996) J Mater Sci: Mater Med, 7, pp. 425-429; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Lopes, M.A., Knowles, J.C., Hing, K., Santos, J.D., Monteiro, F.J., Olsen, I., Sedel, L., Rey, C., Biological evaluation of glass reinforced hydroxyapatite by flow cytometry (1997) Bioceramics, 10, pp. 575-578. , Paris, Oxford, UK: Elsevier; 
Kirkpatrick, C.J., Mittermayer, C., Theoretical and practical aspects of testing potential biomaterials in vitro (1990) J Mater Sci: Mater Med, 1, pp. 9-13; 
Marie, P.J., De Vernejoul, M.C., Lomri, A., Stimulation of bone formation in osteoporosis patients treated with fluoride associated with increased DNA synthesis by osteoblastic cells in vitro (1992) J Bone Miner Res, 7, pp. 103-113; 
Davies, J.E., In vitro modelling of the bone-implant interface (1996) Anat Rec, 245, pp. 426-445; 
Verrier, S., Bareille, R., Rovira, A., Dard, M., Amdee, J., Human osteoprogenitor responses to orthopaedic implant: Mechanism of cell attachment and cell adhesion (1996) J Mater Sci: Mater Med, 17, pp. 46-51; 
Sumner, H., Abraham, D., Bou-Gharios, G., Plater-Zyberk, C., Olsen, I., Simultaneous measurement of cell surface and intracellular antigens by multiple flow cytometry (1991) J Immun Meth, 136, pp. 259-267; 
Bou-Gharios, G., Osman, J., Black, C., Olsen, I., Excess matrix accumulation in Scleroderma is caused partly by differential regulation of stromelysin and TIMP-1 synthesis (1994) Clin Chim Acta, 231, pp. 69-78; 
Kuru, L., Parkar, M.H., Griffiths, G.S., Newman, H.N., Olsen, I., Flow cytometry analysis of functionally distinct gingival and periodontal ligament cells (1998) J Dent Res, 77, pp. 555-564; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of the effects of glass on the biocompatibility of plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings (1999) J Biomed Mater Res, 47, pp. 603-611
},
	month = {2000///},
	pages = {1165 - 1172},
	publisher = {Elsevier Science Ltd},
	abstract = {Human osteoblast-like cells were plated on hydroxyapatite and P                        2O
                        5-glass reinforced hydroxyapatite composite discs. They were also cultured in the presence of media obtained by incubating the discs in the absence of cells. The effects of these direct and indirect interactions were examined by measuring cell proliferation and the expression of certain key extracellular matrix antigens. One composite was found to initially delay cell growth, while the extract of a different composite appeared to down-regulate DNA synthesis. Flow cytometry analysis showed that growth directly on the discs had little effect on collagen type I, but reduced fibronectin and osteocalcin levels. The extracts of the materials generally had less effect, although one extract obtained from the glass-reinforced hydroxyapatite significantly down-regulated fibronectin. These in vitro studies thus suggest that there were only few differences overall in the growth of the cells directly on the glass-reinforced compared with the hydroxyapatite discs and also only relatively small effects of the extracts on the cells. However, the flow cytometry results suggest that both the materials and the extracts may have a potentially important influence on connective tissue production, and that these effects are both material- and antigen-specific. Copyright (C) 2000 Elsevier Science Ltd.
Human osteoblast-like cells were plated on hydroxyapatite and P
                        2O
                        5-glass reinforced hydroxyapatite composite discs. They were also cultured in the presence of media obtained by incubating the discs in the absence of cells. The effects of these direct and indirect interactions were examined by measuring cell proliferation and the expression of certain key extracellular matrix antigens. One composite was found to initially delay cell growth, while the extract of a different composite appeared to down-regulate DNA synthesis. Flow cytometry analysis showed that growth directly on the discs had little effect on collagen type I, but reduced fibronectin and osteocalcin levels. The extracts of the materials generally had less effect, although one extract obtained from the glass-reinforced hydroxyapatite significantly down-regulated fibronectin. These in vitro studies thus suggest that there were only few differences overall in the growth of the cells directly on the glass-reinforced compared with the hydroxyapatite discs and also only relatively small effects of the extracts on the cells. However, the flow cytometry results suggest that both the materials and the extracts may have a potentially important influence on connective tissue production, and that these effects are both material- and antigen-specific.
},
	keywords = {Animal cell culture, antigen expression, Antigens, article, Biocompatibility, Biocompatible Materials, Biomaterials, Bone, Bone cells, cell antigen, Cell culture, Cell Division, cell function, cell growth, Cell Line, cell proliferation, cell structure, collagen type 1, controlled study, DNA, DNA Replication, DNA synthesis, extracellular matrix, Fibronectin, flow cytometry, glass, Glass fibers, Glass-reinforced hydroxyapatite, Growth kinetics, human, human cell, Humans, hydroxyapatite, Hydroxyapatites, immunofluorescence, In vitro, Microscopy, Electron, Scanning, osteoblast, Osteoblasts, osteocalcin, Phosphate minerals, Phosphates, phosphorus pentoxide, priority journal, Proteins, Reinforcement, Scanning electron microscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034059787\&partnerID=40\&md5=898e049d86a10d2797dbd2aa59029fd6},
	author = {Lopes, M.A. and Knowles, J.C. and Santos, J.D. and Monteiro, F.J. and Olsen, I.}
}
@article {2561,
	title = {Flow cytometry analysis of the effects of pre-immersion on the biocompatibility of glass-reinforced hydroxyapatite plasma-sprayed coatings},
	journal = {BiomaterialsBiomaterials},
	volume = {21},
	year = {2000},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(99)00249-5PubMed ID: 10721750Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University College London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United Kingdom; email: iolsen@eastman.ucl.ac.ukChemicals/CAS: Biocompatible Materials; bone sialoprotein; Coated Materials, Biocompatible; Durapatite, 1306-06-5; Fibronectins; SialoglycoproteinsManufacturers: Plasma Biotal, United KingdomReferences: Passuti, N., Daculsi, G., Martin, S., Macroporous calcium phosphate ceramics for long bone surgery in human and dogs - Clinical and histological studies (1990) Clinical Implant Materials, pp. 255-258. , V. Soltesz, \& A. Lee. Amsterdam: Elsevier Science Publishers;Legeros, R., Calcium phosphate materials in restorative dentistry (1988) A Review. Adv Dent Res, 2, pp. 164-180; 
Hench, L.L., Bioactive ceramics: From concept to clinic (1992) J Am Ceram Soc, 74, pp. 1487-1510; 
Conish, H., Aoki, H., Sawai, K., (1981) Science and Medical Applications of Hydroxyaptite, , editors Tokyo: Takyama Press System Centre Co; 
Raemdonck, W., Ducheyne, P., Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Materials, pp. 143-166. , P. Ducheyne, \& G.W. Hastings. Boca Raton, FL, USA: CRC Press; 
Rey, C., Freche, M., Heughebaert, M., Vignoles, M., Apatite chemistry in biomaterial preparation, shaping and biological behaviour (1991) Fourth Symposium on Ceramics in Medicine - Bioceramics 4, pp. 57-64. , G.W. Hastings, \& K.E. Tanner. London: Butterworth-Heinemann; 
Nagase, M., Abe, Y., Udagwa, E., Toxicity of silica-containing calcium phosphate glasses demonstrated in mice (1992) Biomaterials, 13, pp. 172-175; 
Burnie, J., Gilchrist, T., Controlled released glass - A new biomaterial (1983) Ceramics in Surgery, pp. 169-176. , P. Vicenzini. Amsterdam: Elsevier Science Publishers; 
Burnie, F., Gilchrist, T., (1983) Ceramics in Surgery, pp. 177-180. , P. Vicenzini. Amsterdam: Elsevier Science; 
Rawlings, R.D., Rogers, P.S., Stokes, P.M., Glass-ceramics containing apatite (1987) High Tech Ceramics, pp. 73-82. , P. Vicenzini. Amsterdam: Elsevier Science Publishers; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterization of glass-reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Santos, J.D., Lakhan, J.J., Monteiro, F.J., Surface modifications of glass-reinforced hydroxyapatite composites (1995) Biomaterials, 16, pp. 521-526; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., In vitro bioactivity and osteocompatibility evaluation of plasma sprayed hydroxyapatite/glass composite coatings onto Ti-6Al-4V substrates (1997) Ninth International Conference on Biomedical Engineering, pp. 557-559. , In: Goh JCH, editor Singapore; 
Klein, C.P.A.T., Patka, P., Van Der Lubbe, H.B.M., Plasma-sprayed coatings of tetracalciumphosphate, hydroxyl-apatite, and α-TCP on titanium alloy: An interface study (1991) J Biomed Mater Res, 25, pp. 53-65; 
Jansen, J.A., Van Der Waerden, J.P., Wolke, J.G., Histologic investigation of the biologic behavior of different hydroxyapatite plasma-sprayed coatings in rabbits (1993) J Biomed Mater Res, 4, pp. 603-610; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., The ultrastructure of the bone-hydroxyapatite interface in vitro (1992) J Biomed Mater Res, 26, pp. 1365-1382; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., Comparative biological properties of HA plasma-sprayed coatings having different crystallinities (1994) J Mater Sci: Mater Med, 5, pp. 11-17; 
Lugscheider, E., Knepper, M., Heimberg, B., Dekker, A., Kirkpatrick, C.J., Cytotoxicity investigations of plasma-sprayed calcium-phosphate coatings (1994) J Mater Sci: Mater Med, 5, pp. 371-375; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., Comparison of the early production of extracellular matrix on dense hydroxyapatite and hydroxyapatite-coated titanium in cell and organ culture (1995) Biomaterials, 16, pp. 489-495; 
Labat, B., Chanson, A., Frey, J., Effects of γ-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts (1995) J Biomed Mater Res, 29, pp. 1397-1401; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., Adult human bone cells derived from jaw bones cultured on plasma-sprayed or polished surfaces to titanium or hydroxyapatite discs (1996) J Mater Sci: Mater Med, 7, pp. 21-28; 
Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Ariola, C.R., Granchi, D., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin Mater, 15, pp. 173-190; 
Puleo, D.A., Preston, K.E., Shaffer, J.B., Bizios, R., Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques (1993) Biomaterials, 14, pp. 111-114; 
Clifford, C.J., Downes, S., A comparative study of the use of colorimetric assays in the assessment of biocompatibility (1996) J Mater Sci: Mater Med, 7, pp. 637-643; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P 2O 5 glass hydroxyapatite double layer plasma sprayed coatings: In vitro evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced HA plasma sprayed coatings (1999) J Mater Sci: Mater Med, 10, pp. 567-576; 
Macey, M.G., (1994) Flow Cytometry - Clinical Applications, , editor Oxford: Blackwell Scientific; 
Shapiro, H.M., (1995) Practical Flow Cytometry, , New York: Wiley-Liss; 
Melamed, M.R., Lindmo, T., Mandelsohn, M.L., (1990) Flow Cytometry and Sorting, , editors New York: Wiley-Liss; 
Muirhead, K.A., Horan, P.K., Applications of flow cytometry to tissue culture systems (1983) Adv Cell Culture, 3, pp. 57-91; 
Corver, W.E., Cornelisse, C.J., Fleuren, G.J., Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin and propidium iodide on a standard FACScan (1994) Cytometry, 15, pp. 117-128; 
Pande, G., Kumar, N.A., Manogaran, P.S., Flow cytometric study of changes in intracellular free calcium during the cell cycle (1996) Cytometry, 24, pp. 55-63; 
Kamiya, I., Okuda, K., Hara, K., Flow cytometric identification and detection of Porphyromonas gingivalis by a LPS specific monoclonal antibody (1994) J Periodont, 65, pp. 309-315; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of the effects of glass on the biocompatibility of plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings J Biomed Mater Res, , in press; 
Cowles, E.A., DeRome, M.E., Pastizzo, G., Brailey, L.L., Gronowicz, G.A., Mineralization and the expression of matrix proteins during in vivo bone development (1998) Calcif Tissue Int, 62, pp. 74-82; 
Globus, R.K., Dotty, S.B., Lull, J.C., Holmuhamedov, E., Humphries, M.J., Damsky, C.H., Fibronectin is a survival factor for differentiated osteoblasts (1998) J Cell Sci, 111, pp. 1385-1393; 
Clover, J., Gowen, M., Are MG63 and HOS TE 85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? (1994) Bone, 15, pp. 585-591; 
Wojciech, G., Melamed, M.R., Darzynkiewicz, Z., Analysis of apoptosis by flow cytometry (1998) Flow Cytometry Protocols, pp. 217-238. , M.J. Jaroszeski, \& R. Heller. Totowa, NJ, USA: Humana Press
},
	month = {2000///},
	pages = {813 - 820},
	publisher = {Elsevier Science Ltd},
	abstract = {Multilayered coatings composed of mixtures of HA and P                        2O
                        5-based bioactive glasses are of potential clinical benefit in orthopaedic and dental surgery. Pre-immersion of these materials has been reported to further enhance their efficacy in vivo, although the precise biological effects of this treatment are not yet known. In this study we have therefore prepared double-layer plasma-sprayed coatings and evaluated the effects of pre-immersion on the growth and function of human osteosarcoma cells in vitro, using the MTT assay and flow cytometry analysis, respectively. The results showed that the increase in numbers of viable cells was the same or elevated following incubation on the pre-immersed HA and glass-reinforced HA coatings compared with the non-immersed materials. In addition, the expression of bone sialoprotein and fibronectin, two key connective tissue antigens, was up-regulated in cultures grown on the pre-immersed surfaces compared with the non-treated materials. Moreover, cell numbers and antigen expression both improved as the proportion of glass increased, particularly in the pre-immersed samples. Our findings thus suggest that the immersion treatment of these materials appeared to improve the response of these bone-like cells. Copyright (C) 2000 Elsevier Science Ltd.
Multilayered coatings composed of mixtures of HA and P
                        2O
                        5-based bioactive glasses are of potential clinical benefit in orthopaedic and dental surgery. Pre-immersion of these materials has been reported to further enhance their efficacy in vivo, although the precise biological effects of this treatment are not yet known. In this study we have therefore prepared double-layer plasma-sprayed coatings and evaluated the effects of pre-immersion on the growth and function of human osteosarcoma cells in vitro, using the MTT assay and flow cytometry analysis, respectively. The results showed that the increase in numbers of viable cells was the same or elevated following incubation on the pre-immersed HA and glass-reinforced HA coatings compared with the non-immersed materials. In addition, the expression of bone sialoprotein and fibronectin, two key connective tissue antigens, was up-regulated in cultures grown on the pre-immersed surfaces compared with the non-treated materials. Moreover, cell numbers and antigen expression both improved as the proportion of glass increased, particularly in the pre-immersed samples. Our findings thus suggest that the immersion treatment of these materials appeared to improve the response of these bone-like cells.
},
	keywords = {antigen expression, article, Bioactive glasses, Biocompatibility, Biocompatible Materials, Biomaterials, Cell culture, Cell Division, cell growth, cell proliferation, Cell size, Coated Materials, Biocompatible, controlled study, cytology, Cytoplasmic Granules, Dental Materials, drug manufacture, Durapatite, Fibronectin, Fibronectins, flow cytometry, Flow cytometry analysis, glass, Glass fibers, Glass-reinforced hydroxyapatite, Growth kinetics, human, human cell, Humans, hydroxyapatite, in vitro study, materials testing, orthopedics, Osteosarcoma, osteosarcoma cell, Phosphates, Plasma spraying, Pre-immersion, priority journal, protein expression, Proteins, Sialoglycoproteins, sialoprotein, Sprayed coatings, Surface Properties, technique, Tumor Cells, Cultured},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034004660\&partnerID=40\&md5=4f46f4bb4619ff65dccbfcc0fdcd03b1},
	author = {Ferraz, M.P. and Knowles, J.C. and Olsen, I. and Monteiro, F.J. and Santos, J.D.}
}
@article {2560,
	title = {Microstructural dependence of Young{\textquoteright}s and shear moduli of P                    2O
                    5 glass reinforced hydroxyapatite for biomedical applications
},
	journal = {BiomaterialsBiomaterials},
	volume = {21},
	year = {2000},
	note = {Cited By (since 1996): 32Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(99)00248-3PubMed ID: 10711972Language of Original Document: EnglishCorrespondence Address: Lopes, M.A.; INEB Inst. de Engenharia Biomedica, Laboratorio de Biomaterials, Rua do Campo Alegre 823, 4150 Porto, PortugalChemicals/CAS: Biocompatible Materials; Hydroxyapatites; PhosphatesReferences: Burstein, A., Wright, T.M., (1994) Fundamentals of Orthopaedic Biomechanics, , Lippincott: Williams \& Wilkins;Carter, D.R., Giori, N.J., Effect of mechanical stresses on tissue differentiation in the bony implant bed (1991) In the Bone-material Interface, pp. 367-379. , J.E. Davies. Toronto: University of Toronto Press; 
(1995) E - Advanced Technical Ceramics, (PART 2). , European prestandard, ref. no. ENV 843-2:; 
Schreiber, E., Anderson, O.L., Soga, N., (1989) Elastic Constant and Their Measurements, , New York: McGraw-Hill; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterisation of glass reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Knowles, J.C., Talal, S., Santos, J.D., Sintering effects in a glass reinforced hydroxyapatite (1996) Biomaterials, 17, pp. 1437-1442; 
Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Biological evaluation of glass reinforced hydroxyapatite by flow cytometry (1998) Bioceramics, 10, pp. 575-578; 
Rice, R.W., Microstructure dependence of mechanical behaviour (1977) Treatise on Materials Science and Technology, 11, pp. 200-382. , MacCrone R.K. New York: Academic Press; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Murtage, B., (1981) Advanced Linear Programming, , New York: McGraw-Hill; 
Ch{\'a}tal, V., (1990) Linear Programming, , New York: W. H. Freeman; 
Tampieri, A., Celotti, G., Szontagh, F., Landi, E., Sintering and characterisation of hydroxyapatite and tricalcium phosphates with control of their strength and phase porosity (1997) J Mater Sci, 8, pp. 29-37; 
Knowles, J.K., Development of hydroxyapatite with enhanced mechanical properties: Effect of high glass additions on mechanical properties and phase stability of sintered hydroxyapatite (1994) Br Ceram Trans, 93, pp. 100-103; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , Japanese Association of Apatite Science. Tokyo: Takayama Press System Center Co. Inc; 
Sax, M., Zografou, C., Telle, R., Kalawrytimos, G., Properties of sintered apatite ceramics (1995) Ceramic Processing Science and Technology, Ceramic Transactions, 51, pp. 701-705. , In: Hausner H, editor; 
Katz, J.L., Akers, A.S., Germiller, J.A., Obremski, S.M., Singh, S., The elastic properties of apatites and tricalcium phosphates-calcite mixtures (1989) Bioceramics, 1, pp. 86-91; 
Dewith, G., Vandijk, J.A., Hattu, N., Prijs, K., Preparation, microstructure and mechanical properties of dense polycrystalline hydroxyapatite (1981) J Mater Sci, 16, pp. 1592-1598; 
Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812
},
	month = {2000///},
	pages = {749 - 754},
	publisher = {Elsevier Science Ltd},
	abstract = {P                        2O
                        5 glass reinforced hydroxyapatite composite materials were prepared through a liquid-phase sintering process. Secondary phases, β- and α- tricalcium phosphate (β-TCP and α-TCP), were formed in the microstructure of the composites, due to the reaction between the liquid glassy phase and the hydroxyapatite matrix. The dynamic Young{\textquoteright}s modulus (E) and shear modulus (G) of these composites were determined using an impulse excitation method. By applying the Duckworth-Knudsen equation, the elastic property results were correlated with the relative proportion of β-TCP and α-TCP phases and with the porosity percentage present in the microstructure. Glass reinforced hydroxyapatite composites showed lower Young{\textquoteright}s and shear moduli than unmodified hydroxyapatite, mainly due to presence of β-TCP phase. The Duckworth-Knudsen model demonstrated an exponential dependence of E and G modulus with porosity and mathematical equations were derived for composite materials with porosity correction factors (b) of 4.04 and 4.11, respectively, indicating that porosity largely decreased both E and G moduli. (C) 2000 Elsevier Science Ltd.
                        2O
                        5 glass reinforced hydroxyapatite composite materials were prepared through a liquid-phase sintering process. Secondary phases, β- and α-tricalcium phosphates (β-TCP and α-TCP), were formed in the microstructure of the composites, due to the reaction between the liquid glassy phase and the hydroxyapatite matrix. The dynamic Young{\textquoteright}s modulus (E) and shear modulus (G) of these composites were determined using an impulse excitation method. By applying the Duckworth-Knudsen equation, the elastic property results were correlated with the relative proportion of β-TCP and α-TCP phases and with the porosity percentage present in the microstructure. Glass reinforced hydroxyapatite composites showed lower Young{\textquoteright}s and shear moduli than unmodified hydroxyapatite, mainly due to the presence of β-TCP phase. The Duckworth-Knudsen model demonstrated an exponential dependence of E and G modulus with porosity and mathematical equations were derived for composite materials with porosity correction factors (b) of 4.04 and 4.11, respectively, indicating that porosity largely decreased both E and G moduli.
},
	keywords = {article, Biocompatible Materials, Ceramic matrix composites, Chemical analysis, chemical composition, composite graft, Duckworth-Knudsen model, Elastic moduli, Elasticity, glass, hydroxyapatite, Hydroxyapatite glass composites, Hydroxyapatites, Implants (surgical), Mathematical models, Microstructure, Molecular Structure, Phosphate minerals, Phosphates, porosity, priority journal, Shear modulus, shear stress, young modulus, Young{\textquoteright}s modulus},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033975337\&partnerID=40\&md5=3822598d9be6e02a2a07e3465efe1ab6},
	author = {Lopes, M.A. and Silva, R.F. and Monteiro, F.J. and Santos, J.D.}
}
@article {2562,
	title = {Object recognition in image sequences with cellular neural networks},
	journal = {NeurocomputingNeurocomputing},
	volume = {31},
	year = {2000},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: ScopusCODEN: NRCGEdoi: 10.1016/S0925-2312(99)00177-0Language of Original Document: EnglishCorrespondence Address: Buker, U.; Heinz Nixdorf Institute, University of Paderborn, Department of Electrical Engineering, 33095 Paderborn, Germany; email: bueker@get.uni-paderborn.deReferences: Bradski, G., Grossberg, S., Fast-learning VIEWNET architectures for recognizing three-dimensional objects from multiple two-dimensional views (1995) Neural Networks, 8 (7-8), pp. 1053-1080;B{\"u}ker, U., Hartmann, G., Knowledge based view control of a neural 3D object recognition system (1996) In: Proceedings of the 13th International Conference on Pattern Recognition, ICPR{\textquoteright}96, Wien, 4, pp. 24-29. , IEEE Computer Soc. Press, Los Alamitos; 
B{\"u}ker, U., Hybrid object models: combining symbolic and subsymbolic object recognition strategies (1998) In: Proceedings of the 4th International Conference on Information Systems, Analysis, and Synthesis, 1, pp. 444-451. , IIIS, Orlando; 
B{\"u}lthoff, H., Little, J., Poggio, T., A parallel algorithm for real-time computation of optical flow (1989) Nature, 337, pp. 549-553; 
B{\"u}lthoff, H., Edelman, S., Tarr, M., How are three-dimensional objects represented in the brain? (1994) Technical Report, A.I.Memo N1479, , Massachusetts Institute of Technology, A.I. Lab; 
Chua, L.O., Yang, L., Cellular neural networks: Theory (1988) IEEE Trans. Circuits Systems, 35, pp. 1257-1272; 
Chua, L.O., Roska, T., The CNN Paradigm (1993) IEEE Trans. Circuits Systems (Part I) CAS-40, 3, pp. 559-577; 
Fu, L.M., Analysis of the dimensionality of neural networks for pattern recognition (1990) Pattern Recognition, 23, pp. 1131-1140; 
Grassi, G., A new approach to design cellular neural networks for associative memories (1997) IEEE Trans. Circuits Systems - I Fundam. Theory Appl., 44 (9), pp. 362-366; 
Hartmann, G., Dr{\"u}e, S., Dunker, J., Kr{\"a}uter, K.O., Mertsching, B., Seidenberg, E., The SENROB Vision-System and its Philosophy (1994) In: Proceedings of the 12th International Conference on Pattern Recognition ICPR{\textquoteright}94, 2, pp. 573-576. , Jerusalem, IEEE Computer Soci. Press, Los Alamitos; 
Hartmann, G., B{\"u}ker, U., Dr{\"u}e, S., A hybrid neuro-artificial intelligence architecture (1999) Handbook on Computer Vision and Applications, 3, pp. 153-196. , in: B. J{\"a}hne et al. (Eds.), Academic Press, San Diego; 
Hebert, M., (1995) Object Recognition in Computer Vision, , (Eds.), Springer, New York; 
Kikuchi, M., Fukushima, K., Neural network model of the visual system: Binding: Form and motion (1996) Neural Networks, 9 (8), pp. 1417-1427; 
Kosslyn, S.M., (1995) Image and Brain, , Cambridge: MIT Press; 
Lin, W.G., Wang, S.S., A new neural model for invariant pattern recognition (1996) Neural Networks, 9 (5), pp. 899-913; 
Little, J., Boyd, J., Describing motion for recognition (1995) IEEE Symp. Comput. Vision, pp. 235-240; 
Little, J., Boyd, J., (1997) Recognizing People by their Gait: The Shape of Motion, , http://www-vision.ucsd.edu/, On-line document; 
Liu, D., Michel, A., Sparsely interconnected neural networks for associative memories with applications to cellular neural networks (1994) IEEE Trans. Circuits Systems - II Analog Digital Signal Process, 41 (4), pp. 295-307; 
Liu, D., Cloning template design of cellular neural networks for associative memories (1997) IEEE Trans. Circuits Systems - I Fundam. Theory Appl., 44 (7), pp. 646-650; 
Liu, D., Lu, Z., A new synthesis approach for feedback neural networks based on the perceptron training algorithm (1997) IEEE Trans. Neural Networks, 8 (6), pp. 1468-1482; 
Michel, A., Jie Si, Yen, G., Analysis and Synthesis of a Class of Discrete-Time Neural Networks Described on Hypercubes (1991) IEEE Trans. Neural Networks, 2 (1), pp. 32-46; 
Pandya, A., MacY, R., (1996) Pattern Recognition with Neural Networks in C++, , CRC Press, Florida; 
Rekecsky, C., Roska, T., Ushida, A., CNN-based difference-controlled adaptive non-linear image filters (1998) Internat. J. of Circuit Theory Appl., 26 (4), pp. 374-423; 
Roska, T., Vandewalle, J., (1993) Cellular Neural Networks, , John Wiley \& Sons, New York; 
Szolgay, P., Szatmari, I., Laszlo, K., A Fast Fixed Point Learning Method to Implement Associative Memory on CNN{\textquoteright}s (1997) IEEE Trans. Circuits Systems-I: Fundamental Theory and Applications, 44 (4), pp. 362-366; 
Treisman, A., A feature-integration theory of attention (1980) Cognitive Psychology, 12, pp. 97-136; 
Tn, S., Hao, J., Vanderwalle, J., Cellular neural networks as a model of associative memories (1990) Proceedings of the CNNA-90, pp. 26-35. , Budapest, IEEE, New York
},
	month = {2000///},
	pages = {125 - 141},
	publisher = {Elsevier Science B.V.},
	abstract = {In this paper, the application of CNN associative memories for 3D object recognition is presented. The main idea is to analyse the optical flow in an image sequence of an object. Several features of the optical flow between two succeeding images are calculated and merged to a time series of features for the whole image sequence. These features show several object specific characteristics and are used for a classification step in an object recognition system. Therefore, the feature vectors of an object set are learnt and recalled by an associative memory based on the paradigm of cellular neural networks (CNN). (C) 2000 Elsevier Science B.V.In this paper, the application of CNN associative memories for 3D object recognition is presented. The main idea is to analyze the optical flow in an image sequence of an object. Several features of the optical flow between two succeeding images are calculated and merged to a time series of features for the whole image sequence. These features show several object specific characteristics and are used for a classification step in an object recognition system. Therefore, the feature vectors of an object set are learnt and recalled by an associative memory based on the paradigm of cellular neural networks (CNN).
},
	keywords = {algorithm, artificial neural network, Associative memory, Associative storage, Cellular neural networks, Computational methods, computer system, Image analysis, Image sequences, mathematical computing, Object recognition, Optical flow, Optical flows, Pattern recognition, Pattern recognition systems, priority journal, review, Three dimensional computer graphics, Time series analysis, Vectors},
	isbn = {09252312 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0343852159\&partnerID=40\&md5=ecb858090fa38d7d39e6142515efac59},
	author = {Milanova, M. and B{\"u}ker, U.}
}
@conference {2554,
	title = {Remote foetal heart rate acquisition and analysis},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {Computers in Cardiology 2000},
	year = {2000},
	note = {Conference code: 45063Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: COCADLanguage of Original Document: EnglishCorrespondence Address: Marques, P.; INEB - Instituto de Engenharia, Biomedica, Porto, Portugal},
	month = {2000///},
	pages = {573 - 574},
	publisher = {IEEE},
	organization = {IEEE},
	address = {Cambridge, MA, USA},
	abstract = {The remote foetal monitoring and analysis is part of INTERCARE project - an integrated healthcare system applied to Electrocardiography and Cardiotocography. Its aim is to provide low cost and wide spread technological infrastructure that supports an integrated healthcare system to users and healthcare providers. This paper illustrates the work done in the cardiotocography area. We have developed a system for remote acquisition and analysis of FHR. The system uses a portable {\textquoteright}foetal detector{\textquoteright} which can be used by the pregnant woman to acquire the heart beats. This detector sends a modulated audio signal via the telephone line or a computer connected to Internet. At the processing end the signal received by the telephone can be switched to the computer sound acquisition board. The signal is then processed by an application that reads from the soundcard. Due to the noise added by devices and telephone lines, the signal is filtered using optimal FIR filters and is decimated until its band of interest covers the peaks of the heart sounds. Next the signal is processed by a specially developed adaptive correlation algorithm in order to obtain the heart beat instants. The whole process operates in real time. The adaptive correlation algorithm performs a continuous adjustment of the heart beat template and threshold detection levels.},
	keywords = {Adaptive algorithms, Adaptive correlation algorithm, Cardiology, Cardiotocography, Client server computer systems, Data acquisition, Data warehouse, Database systems, Electrocardiography, FIR filters, Foetal heart rate, Internet, Patient monitoring, Remote foetal monitoring, Signal filtering and prediction, Sonoscope, Spurious signal noise, Telephone},
	isbn = {02766574 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034474836\&partnerID=40\&md5=c85d04aba26fdb2f92645c8b762f8c40},
	author = {Marques, P. and Marques de Sa, J.P. and Bernardes, J. and Sousa, P.}
}
@article {2574,
	title = {CaO-P                    2O
                    5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation
},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {45},
	year = {1999},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19990615)45:4<376::AID-JBM13>3.0.CO;2-SLanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; Departamento Engenharia Metalurgica, FEUP, Universidade do Porto, R. dos Bragas, 4099 Porto Codex, PortugalChemicals/CAS: Alloys; Calcium, 7440-70-2; CaO-SiO2-P2O5-CaF2 glass; Coated Materials, Biocompatible; Durapatite, 1306-06-5; Phosphates; Powders; Resin Cements; Solutions; titanium alloy (TiAl6V4), 12743-70-3; Titanium, 7440-32-6References: Denissen, H., Mangano, C., Venini, G., (1985) Hydroxylapatite Implants., , Padua: Piccin Nuova Libraria, S.P.A;Van Raemdonck, W., Ducheyne, P., De Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Biomaterials, pp. 143-166. , Ducheyne P, Hastings GW, editors, Boca Raton, FL: CRC Press; 
Legeros, R.Z., Incorporation of magnesium in synthetic and biological apatites (1994) Tooth Enamel IV., pp. 32-36. , Fearnead RW, editor. Amsterdam: Elsevier Science; 
Jha, L.J., Best, S.M., Knowles, J.C., Rehman, I., Santos, J.D., Bonfield, W., Preparation and characterization of fluoride-substituted apatites (1997) J Mater Sci Mater Med, 8, pp. 185-191; 
Geesink, R.G.T., Degroot, K., Chemical implant fixation using hydroxyl-apatite coatings (1987) Clin Orthop, 2, pp. 147-170; 
Radin, S., Ducheyne, P., The effect of plasma sprayed induced changes in the characteristics on the in vitro stability of calcium phosphate ceramics (1990) Transactions of the 16th Annual Meeting of the Society for Biomaterials XIII, pp. 128-130; 
Cook, S.D., Thomas, K.A., Hydroxyapatite coated porous titanium for use as an orthopaedic biologic attachment (1989) Clin Orthop, 230, pp. 303-312; 
McPherson, R., Gane, N., Bastow, T.J., Structural characterization of plasma-sprayed hydroxylapatite coatings (1995) J Mater Sci Mater Med, 6, pp. 327-334; 
De Groot, K., Geesink, R., Klein, C.P.A.T., Serekian, P., Plasma sprayed coatings of hydroxylapatite (1987) J Biomed Mater Res, 21, pp. 1375-1381; 
Ravagliolli, A., Krajewszki, A., Laudadio, P., Presutti, L., Cunsolo, E., Martinetti, R., About ceramics for stapedial prostheses and the ossicular chain (1992) Fifth International Symposium on Ceramics in Medicine-Bioceramics 5, pp. 451-458. , Yamamuro T, Kokubo T, Nakamura T, editors. Kyoto: Kobunshi Kankokai; 
Hastings, G.W., Daily, D., Hydroxyapatite coatings (1989) First International Symposium on Ceramics in Medicine-Bioceramics 1, pp. 355-358. , Oonishi H, Aoki H, Sawai K, editors. Tokyo: Ishiyaku EuroAmerica; 
Aoki, H., Medical applications of hydroxyapatite (1991) Science and Medical Applications of Hydroxyaptite, pp. 175-192. , Aoki H, Sawai K, editors. Tokyo: Takyama Press System Centre; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Evans, G., Behiri, J., Currey J, B.W., Microhardness and young{\textquoteright}s modulus in cortical bone exhibiting a wide range of mineral volume fractions, and in a bone analogue (1990) J Mater Sci Mater Med, 1, pp. 38-43; 
Rey, C., Froche, M., Heughebaert, M., Heughebaert, J.C., Lacout, J.L., Vignoles, M., Apatite chemistry in biomaterial preparation, shaping and biological behaviour (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, pp. 57-60. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Knowles, J.C., Talal, S., Santos, J.D., Sintering effects in a glass reinforced hydroxyapatite (1996) Biomaterials, 17, pp. 1437-1442; 
Kokubo, T., Hayashi, T., Sakka, S., Kitsugi, T., Yamamuro, T., Takagi, M., Shibuya, T., Surface structure of a load bearable bioactive glass-ceramic A-W (1981) High Tech Ceramics, pp. 175-184. , Vicenzini P, editor. Amsterdam: Elsevier Science Publisher BV; 
Hench, L., Anderson, O.H., LaTorre, G.P., The kinetics of bioactive ceramics. Part III: Surface reactions for bioactive glasses compared with inactive glass (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 155-162; 
Daculsi, G., LeGeros, R.Z., Heughebart, M., Barbieux, I., Formation of a carbonate apatite crystals after implantation of calcium phosphate ceramics (1990) Calcif Tissue Int, 46, pp. 20-27; 
Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., Kitsugi, T., Yamamuro, T., Ca-P rich layer formed on high strength bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 331-343; 
Ducheyne, P., Radin, S., Ishikawa, K., Kim, C.S., Dissolution and precipitation of calcium phosphate phases on biomaterials correlates with in vivo bioactivity (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, pp. 135-144. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Rey, C., Hina, A., Amrah-Bouali, S., Ranz, X., Surface reactions of calcium-phosphate bioceramics, comparison with bone mineral surface chemistry (1995) Fourth Euro Ceramics., 8, pp. 301-312. , Ravaglioli A, editor. Faenza: Gruppo Editoriale Faenza Editrice; 
Van Blitterswijk, C.A., Grote, J.J., Kuypers, W., Blok-Van Hoek, C.J.G., Daems, W.T., Bioreactions at the tissue/hydroxyapatite interface (1985) Biomaterials, 6, pp. 243-251; 
Ducheyne, P., Bioceramics: Material characteristics versus in vivo behavior (1987) J Biomed Mater Res, 21, pp. 219-236; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Nakamura, T., Kitsogi, T., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J Biomed Mater Res, 24, pp. 471-488; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions: Structure and properties of the composites (1995) J Mater Sci Mater Med, 6, pp. 348-352; 
Tanahashi, M., Yao, T., Kokubo, T., Minoda, M., Miyamoto, T., Nakamura, T., Yamamuro, T., Apatite coated on organic polymers by biomimetic process: Improvement in adhesion to substrate by HC1 treatment (1995) J Mater Sci Mater Med, 6, pp. 319-326; 
Neo, M., Nakamura, T., Yamamuro, T., Ohtsuki, C., Kokubo, T., Bando, Y., A comparative study of ultrastructures of the interfaces between four kinds of surface-active ceramic and bone (1992) J Biomed Mater Res, 26, pp. 1419-1432; 
Li, P., Ohtsuki, C., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., Process of formation of bone-like apatite layer on silica gel (1993) J Mater Sci Mater Med, 4, pp. 127-131; 
Santos, J.D., Jha, L.J., Monteiro, F.J., Surface modifications of glass-reinforced hydroxyapatite composites (1995) Biomaterials, 16, pp. 521-526; 
Neo, M., Kotani, S., Fujita, Y., Yamamuro, T., Bando, Y., Ohtsuki, C., Kokubo, T., Differences in ceramic-bone interface between surface-active ceramics and resorbable ceramics: A study by scanning and transmission electron microscope (1992) J Biomed Mater Res, 26, pp. 255-267; 
Li, P., Yang, Q., Zhang, F., Kokubo, T., The effect of residual glassy phase in a bioactive glass-ceramic on the formation of its surface apatite layer in vitro (1992) J Mater Sci Mater Med, 3, pp. 452-456; 
Kotani, S., Fujita, Y., Kitsugi, T., Nakamura, T., Yamamuro, T., Bone bonding mechanism of β-tricalcium phosphate (1991) J Biomed Mater Res, 25, pp. 1303-1315; 
Daculsi, G., LeGeros Rz, H.M., Barbieux, I., Formation of carbonate-apatite crystals after implantation of calcium phosphate ceramics (1990) Calcif Tissue Int, 46, pp. 20-27; 
Silva, P.L., Monteiro, F.J., Santos, J.D., Knowles, J.C., Adhesion and microstructural characterisation of plasma sprayed hydroxyapatite-glass ceramic coatings onto Ti-6Al-4V substrates (1998) Surf Coatings Technol, 102, pp. 191-196; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Yamamuro, T., Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid (1992) J Biomed Mater Res, 3, pp. 79-83; 
Blakeslee, D.M., Dal-Favero, M.E., (1997) NIST X-ray Photoelectron Spectroscopy Database, , Gaitherburg, MD: National Institute of Standards and Technology; 
Anderson, O.H., Kangasniemi, I., Calcium phosphate formation at the surface of bioactive glass in vitro (1991) J Biomed Mater Res, 25, pp. 1019-1030; 
Santos, J.D., Jha, L.J., Monteiro, F.J., In vitro calcium phosphate formation on SiO 2-Na 2O-CaO-P 2O 5 glass reinforced hydroxyapatite composite: A study by XPS analysis (1996) J Mater Sci Mater Med, 7, pp. 181-185; 
Verhoeven, J.A.T., Van Doveren, H., An XPS investigation of the interface of methane, ethyne, ethene and ethane with barium surface (1982) Surf Sci, 123, pp. 369-383
},
	month = {1999///},
	pages = {376 - 383},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Double-layer composite coatings composed of a P                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
Double-layer composite coatings composed of a P
                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
},
	keywords = {alloy, Alloys, Apatite, article, Atomic absorption spectroscopy, Atomic spectroscopy, Bioactivity, Biocompatibility, biomaterial, Biomaterials, Biomedical engineering, Body fluids, Calcium, Calcium Compounds, CaO SiO2 P2O5 CaF2 glass, CaO-SiO2-P2O5-CaF2 glass, Chemical analysis, chemical composition, Coated Materials, Biocompatible, Double layer coatings, Double-layer coatings, Durapatite, film, glass, Glass plasma spraying, human, Humans, hydroxyapatite, immersion, implant, in vitro study, Ionic solution changes, Magnesium, materials testing, Molybdenum blue, Multilayers, Particle Size, phosphate, Phosphate minerals, Phosphates, Phosphorus Compounds, phosphorus pentoxide, Plasma spraying, potassium, powder, Powders, resin cement, Resin Cements, Scanning electron microscopy, Sodium, solution and solubility, Solutions, structure analysis, Substrates, Surface Properties, surface property, thickness, Tissue, Titanium, titanium alloy (TiAl6V4), Titanium alloys, X ray photoelectron spectroscopy},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033563315\&partnerID=40\&md5=8c0de1e25709302641b203efac50337b},
	author = {Ferraz, M.P. and Monteiro, F.J. and Santos, J.D.}
}
@conference {2566,
	title = {ECG noise filtering using wavelets with soft-thresholding methods},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {The 26th Annual Meeting: Computers in Cardiology 1999},
	year = {1999},
	note = {Conference code: 56519Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: COCADLanguage of Original Document: EnglishCorrespondence Address: Agante, P.M.; Inst Nacional de Engenharia, Biomedica, Oporto, PortugalReferences: Graps, A., An introduction to wavelets (1995) IEEE Computational Science and Engineering, 2 (2);Donoho, D.L., De-noising by soft-thresholding (1992) Symposium on Wavelet Theory, , Vanderbilt; 
Daubechies, I., The wavelet transform, time-frequency localization and signal analysis (1990) IEEE Transactions on Information Theory, 36 (5), pp. 961-1005; 
Chui, C.K., (1992) An Introduction to Wavelets, , Academic Press; 
Willems, J.L., CSE multilead atlas (1988) Common Standards for Quantitative Electrocardiography (European Concerted Action Project II.2.2), Div. Medical Informatics, , CSE Project Leader, Univ. Hosp. Gasthuisberg, Leuven; 
Coifman, R., Meyer, Y., Wickerhauser, V., Wavelet Analysis and Signal Processing, , ceres.math.yale.edu, Yale University, available by anonymous ftp at; 
},
	month = {1999///},
	pages = {535 - 538},
	publisher = {IEEE},
	organization = {IEEE},
	address = {Hannover, Ger},
	abstract = {In this paper we analyze algorithms for filtering white gaussian noise and 50 Hz power line noise from ECG signals. We used several wavelets to study their effect and efficiency in the filtering process. To deal with these different kinds of noises we used two distinct soft-thresholding techniques: the Donoho{\textquoteright}s statistical threshold estimator and a method developed by us. This last method exploits one of the wavelet processing main features: time-frequency relation. The de-noising methods led to good filtering results: noise reduction with only minor change of the ECG waveforms as confirmed by the high correlation values between the processed signal and the original one. These good results are due to a wavelet advantage over classical filtering - time-frequency relation - enabling the possibility of filtering noise in the same frequency band of the ECG signal with minimal interference.},
	keywords = {Algorithms, Electrocardiography, Signal filtering and prediction, Signal interference, Wavelet denoising, White noise},
	isbn = {02766574 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0342854378\&partnerID=40\&md5=11e3b634e77d95bc3486d33a9f2a4b2d},
	author = {Agante Da Silva, P.M.G. and Marques De S{\'a}, J.P.}
}
@article {2571,
	title = {Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P                    2O
                    5 coatings
},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {47},
	year = {1999},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19991215)47:4<603::AID-JBM18>3.0.CO;2-6PubMed ID: 10497297Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University of London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United KingdomChemicals/CAS: Bone Substitutes; Coated Materials, Biocompatible; HydroxyapatitesReferences: Passuti, N., Daculsi, G., Martin, S., Macroporous calcium phosphate ceramics for long bone surgery in human and dogs - Clinical and histological studies (1990) Clinical Implant Materials, pp. 255-258. , Soltesz V, Lee A, editors. Amsterdam: Elsevier Sciesnce Publishers;Legeros, R., Calcium phosphate materials in restorative dentistry. A review (1988) Adv Dent Res, 2, pp. 164-180; 
Hench, L.L., Bioactive ceramics: From concept to clinic (1992) J Am Ceram Soc, 74, pp. 1487-1510; 
Conish, H., Aoki, H., Sawai, K., (1981) Science and Medical Applications of Hydroxyapatite, , Tokyo: Takyama Press System Centre Co; 
Raemdonick, W., Ducheyne, P., Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Materials, pp. 143-166. , Ducheyne P, Hastings GW, editors. Boca Raton, FL: CRC Press; 
Rey, C., Freche, M., Heughebaert, M., Vignoles, M., Apatite chemistry in biomaterial preparation, shaping and biological behaviour (1991) 4th Symposium on Ceramics in Medicine - Bioceramics, 4, pp. 57-64. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Nagase, M., Abe, Y., Udagwa, E., Toxicity of silica-containing calcium phosphate glasses demonstrated in mice (1992) Biomaterials, 13, pp. 172-175; 
Burnie, J., Gilchrist, T., Controlled released glass - A new biomaterial (1983) Ceramics in Surgery, pp. 169-176. , Vicenzini P, editor. Amsterdam: Elsevier Science Publishers; 
Jha, L.J., Santos, J.D., Knowles, J.C., Characterization of apatite layer formation on P 2O 5-CaO, P 2O 5-CaO-Na 2O, P 2O 5-CaO-Na 2O-Al 2O 3 glass hydroxyapatite composites (1996) J Biomed Mater Res, 31, pp. 481-486; 
Rawlings, R.D., Rogers, P.S., Stokes, P.M., Glass-ceramics containing apatite (1987) High Tech Ceramics, pp. 73-82. , Vicenzini P, editor. Amsterdam: Elsevier Science Publishers; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterization of glass-reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Santos, J.D., Lakhan, J.J., Monteiro, F.J., Surface modifications of glass-reinforced hydroxyapatite composites (1995) Biomaterials, 16, pp. 521-526; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., In vitro bioactivity and osteocompatibility evaluation of plasma sprayed hydroxyapatite/glass composite coatings onto Ti-6AL-4V substrates (1997) 9th International Conference on Biomedical Engineering, pp. 557-559. , Goh JCH, editor. Singapore: BAC Printers; 
Klein, C.P.A.T., Patka, P., Van Der Lubbe, H.B.M., Plasma-sprayed coatings of tetracalcium phosphate, hydroxylapatite, and α-TCP on titanium alloy: An interface study (1991) J Biomed Mater Res, 25, pp. 53-65; 
Jansen, J.A., Van Der Waerden, J.P., Wolke, J.G., Histologic investigation of the biologic behavior of different hydroxyapatite plasma-sprayed coatings in rabbits (1993) J Biomed Mater Res, 14, pp. 603-610; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., The ultrastructure of the bone-hydroxyapatite interface in vitro (1992) J Biomed Mater Res, 26, pp. 1365-1382; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., Comparative biological properties of HA plasma-sprayed coatings having different crystallinities (1994) J Mater Sci Mater Med, 5, pp. 11-17; 
Lugscheider, E., Knepper, M., Heimberg, B., Dekker, A., Kirkpatrick, C.J., Cytotoxicity investigations of plasma-sprayed calcium-phosphate coatings (1994) J Mater Sci Mater Med, 5, pp. 371-375; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., Comparison of the early production of extracellular matrix on dense hydroxyapatite and hydroxyapatite-coated titanium in cell and organ culture (1995) Biomaterials, 16, pp. 489-495; 
Labat, B., Chanson, A., Frey, J., Effects of γ-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts (1995) J Biomed Mater Res, 29, pp. 1397-1401; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., Adult human bone cells derived from jaw bones cultured on plasma-sprayed or polished surfaces to titanium or hydroxyapatite discs (1996) J Mater Sci Mater Med, 7, pp. 21-28; 
Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Ariola, C.R., Granchi, D., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin Biomater, 15, pp. 173-190; 
Puleo, D.A., Preston, K.E., Shaffer, J.B., Bizios, R., Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques (1993) Biomaterials, 14, pp. 111-114; 
Clifford, C.J., Downes, S., A comparative study of the use of colorimetric assays in the assessment of biocompatibility (1996) J Mater Sci Mater Med, 7, pp. 637-643; 
Macey, M.G., (1994) Flow Cytometry - Clinical Applications, , Oxford, UK: Blackwell Scientific Publications; 
Shapiro, H.M., (1995) Practical Flow Cytometry, , New York: Wiley-Liss; 
Melamed, M.R., Lindmo, T., Mendelsohn, M.L., (1990) Flow Cytometry and Sorting, , New York: Wiley-Liss; 
Muirhead, K.A., Horan, P.K., Applications of flow cytometry to tissue culture systems (1983) Adv Cell Culture, 3, pp. 57-91; 
Corver, W.E., Cornelisse, C.J., Fleuren, G.J., Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin and propidium iodide on a standard FACScan (1994) Cytometry, 15, pp. 117-128; 
Pande, G., Kumar, N.A., Manogaran, P.S., Flow cytometric study of changes in intracellular free calcium during the cell cycle (1996) Cytometry, 24, pp. 55-63; 
Kamiya, I., Okuda, K., Hara, K., Flow cytometric identification and detection of Porphyromonas gingivalis by a lps specific monoclonal antibody (1994) J Periodontol, 65, pp. 309-315; 
Clover, J., Gowen, M., Are MG63 and HOS TE 85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? (1994) Bone, 15, pp. 585-591; 
Kuru, L., Parkar, M.H., Griffiths, G.S., Newman, H.N., Olsen, I., Flow cytometry analysis of functionally distinct gingival and periodontal ligament cells (1998) J Dent Res, 77, pp. 555-564; 
Abraham, D., Lupoli, S., McWhirter, A., Plater-Zyberk, C., Piela, T.H., Korn, J., Olsen, I., Black, C., Expression and function of surface antigens on scleroderma fibroblasts (1991) Arthritis Rheum, 34, pp. 1164-1172; 
Beauchamp, J.R., Abraham, D.J., Bou-Gharios, G., Partridge, T.A., Olsen, I., Expression and function of adhesion molecules during differentiation of human skeletal muscle in culture (1992) Am J Pathol, 140, pp. 387-401; 
Bou-Gharios, G., Osman, J., Black, C., Olsen, I., Excess matrix accumulation in scleroderma is caused partly by differential regulation of stromelysin and TIMP-1 synthesis (1994) Clin Chim Acta, 231, pp. 69-78; 
Lopes, M.A., Knowles, J.C., Hing, K.A., Santos, J.D., Monteiro, F.J., Olsen, I., Biological evaluation of glass reinforced hydroxyapatite by flow cytometry (1997) 10th Symposium on Ceramics in Medicine-bioceramics, 10, pp. 575-578. , Sedel L, Rey C, editors. Cambridge, UK: University Press; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Sandy, J., Davies, M., Prime, S., Farndale, R., Signal pathways that transduce growth factor-stimulated mitogenesis in bone cells (1998) Bone, 23, pp. 17-26
},
	month = {1999///},
	pages = {603 - 611},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Multilayered coatings composed of mixtures of hydroxyapatite (HA) and P                        2O
                        5-based bioactive glasses offer potential clinical benefits in orthopedic and dental surgery. In this study double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The cells were cultured on the materials and the effects on cell growth were determined using a spectrophometric assay of a mitochondrial enzyme that is active in viable cells. While none of the materials influenced the growth of the MG63 cells, the HOS cells appeared to undergo less proliferation on all the HA materials. Flow cytometry analysis was carried out using rabbit antibodies against osteonectin, osteopontin, bone sialoprotein, fibronectin, and collagen type I to measure the effects of the materials on key cellular functions. The results showed that the materials downregulated the expression of these extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells.
In this study, double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The materials downregulated the expression of the extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells.
},
	keywords = {Animals, Antibodies, antigen expression, article, Bioassay, Biomaterials, Bone cells, Bone sialoprotein, Bone Substitutes, calcium phosphate, cancer cell culture, Cell Division, cell function, cell granule, cell growth, cell proliferation, Cell size, Cells, Coated Materials, Biocompatible, Collagen, collagen type 1, connective tissue, controlled study, dentistry, enzyme activity, Enzymes, Fibronectin, flow cytometry, Flow cytometry analysis, glass, Growth kinetics, human, human cell, Humans, hydroxyapatite, Hydroxyapatite-glass, Hydroxyapatites, Incubation, Mitochondrial enzyme, orthopedics, Osteoblasts, osteonectin, Osteopontin, Osteosarcoma, osteosarcoma cell, Osteosarcoma cells, Phosphates, Proliferation, Proteins, Rabbits, sialoprotein, Spectrophometric assay, Tissue culture, Tumor Cells, Cultured},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031573504\&partnerID=40\&md5=c6bbcda19f6e76323d16484a9db1c0da},
	author = {Ferraz, M.P. and Knowles, J.C. and Olsen, I. and Monteiro, F.J. and Santos, J.D.}
}
@article {2569,
	title = {Glass-reinforced hydroxyapatite composites: Fracture toughness and hardness dependence on microstructural characteristics},
	journal = {BiomaterialsBiomaterials},
	volume = {20},
	year = {1999},
	note = {Cited By (since 1996): 65Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(99)00112-XPubMed ID: 10535820Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Laboratorio de Biomateriais, Instituto de Engenharia Biomedica, INEB, Rua do Campo Alegre 823, 4150 Porto, PortugalChemicals/CAS: alpha-tricalcium phosphate; Biocompatible Materials; calcium phosphate, 10103-46-5; Calcium Phosphates; Composite Resins; dicalcium phosphate anhydrous, 7757-93-9; Durapatite, 1306-06-5; monocalcium phosphate; tetracalcium phosphateReferences: Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812;Martin, R.I., Brown, P.W., Mechanical properties of hydroxyapatite formed at physiological temperature (1995) J Mater Sci Mater Med, 6, pp. 138-143; 
Aoki, H., (1994) Medical Applications of Hydroxyapatite, pp. 90-173. , Tokyo, Missouri, USA: Ishiyaku EuroAmerica Inc; 
Yamamuro, T., Hench, L., Wilson, J., Calcium phosphate and hydroxyapatite ceramics (1990) In: Handbook of Bioactive Ceramics, 2, pp. 273-335. , editors. Florida: CRC Press Inc; 
Cao, W., Hench, L.L., Bioactive materials (1996) Ceramics Int, 22, pp. 493-507; 
Davidge, R.W., (1979) Mechanical Behaviour of Ceramics, , Cambridge: Cambridge University Press; 
Orange, G., Liang, K.M., Crack resistance and fracture toughness of alumina and zirconia ceramics: comparisons of notched-beam and indent techniques (1988) Science of Ceramics, 14, pp. 709-714. , Amsterdam: Elsevier; 
Niihara, K., Morena, R., Hasselman, D.P.H., Evaluation of K IC of brittle solids by the indentation method with low crack-to-indent ratios (1982) J Mater Sci Lett, 1, pp. 13-16; 
Evans, G., Fracture toughness: The role of indentation techniques (1979) Fracture Mechanics Applied to Brittle Materials ASTM STP 678, pp. 112-135. , S.W. Freiman. Blasksbaurg: American Society for Testing and Materials; 
Anstis, G.R., Chantikul, P., Lawn, B.R., Marshall, D.B., A critical evaluation of indentation techniques for measuring fracture toughness: I. Direct crack measurements (1981) J Am Ceram Soc, 64 (9), pp. 533-538; 
Chantikul, P., Anstis, G.R., Lawn, B.R., Marshall, D.B., A critical evaluation of indentation techniques for measuring fracture toughness: II. Strength method (1981) J Am Ceram Soc, 64 (9), pp. 539-543; 
Evans, G., Charles, E.A., Fracture toughness determinations by indentation (1976) J Am Ceram Soc, 59 (78), pp. 371-372; 
Marion, R.H., Use of indentation fracture to determine fracture toughness (1979) Fracture Mechanics Applied to Brittle Materials, ASTM STP 678, pp. 103-111. , S.W. Freiman. Blasksbaurg: American Society for Testing and Materials; 
Knowles, J.C., Talal, S., Santos, J.D., Sintering effects in a glass reinforced hydroxyapatite (1996) Biomaterials, 17, pp. 1437-1442; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass reinforced hydroxyapatite composites: Secondary phase proportions and densification effects on biaxial bending strength (1999) J Appl Biomater, , in press; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 4, pp. 649-656; 
Santos, D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterisation of glass reinforced hydroxyapatite composites (1994) Biomaterials, 15 (1), pp. 5-10; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39 (2), pp. 244-251; 
Laugier, M.T., New formula for indentation toughness in ceramics (1987) J Mater Sci Lett, 6, pp. 355-356; 
Ampiro, R., (1958) Acta Anat, 3, p. 161; 
De With, G., Van Dijk, H.J.A., Hattu, N., Prijs, K., Preparation, microstructure and mechanical properties of dense polycrystalline hydroxyapatite (1981) J Mater Sci, 16, pp. 1592-1598; 
German, R.M., (1985) Liquid Phase Sintering, , Oxford: Plenum Press; 
Rice, R.W., Microstructure dependence of mechanical behaviour (1977) Treatise on Materials Science and Technology, 11. , MacCrone R.K. New York: Academic Press; 
Rice, R.W., Grain size and porosity dependence of ceramic fracture energy and toughness at 22{\textdegree}C (1996) J Mater Sci, 31, pp. 1969-1983; 
Lankford, J., Indentation microfracture in the Palmqvist crack regime: Implications for fracture toughness evaluation by the indentation method (1982) J Mater Sci Lett, 1, pp. 493-495; 
Li, Z., Ghosh, A.A., Kobayashi, A.S., Bradt, R.C., Indentation fracture toughness of sintered silicon carbide in the Palmqvist crack regime (1989) J Am Ceram Soc, 72 (6), pp. 904-911; 
Lawn, R., Marshall, D.B., Hardness, toughness and brittleness: An indentation analysis (1980) J Am Ceram Soc, 62 (78), pp. 347-350; 
Niihara, K., A fracture mechanics analysis of indentation-induced Palmqvist crack in ceramics (1983) J Mater Sci Lett, 2, pp. 221-223; 
Plannel, J.A., Vallet-Regi, M., Fernandez, E., Driessens, F.C.M., Fracture toughness evaluation of sintered hydroxyapatite (1994) Bioceramics 7, Proceedings of the Seventh International Symposium on Ceramics in Medicine, pp. 18-22. , In: Andersson OH, Yli-Urpo A, editors; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , Japanese Association of Apatite Science. Tokyo: Takayama Press System Center Co. Inc; 
Hauber, R.A., Anderson, R.M., Engineering properties of glass-matrix composites In: Ceramics and Glasses, Engineered Materials Handbook, pp. 858-869. , USA: ASM Publication; 
Barsoum, M., Mechanical properties: Fast fracture (1997) Fundamentals of Ceramics. Series in Materials Science and Engineering, pp. 391-439. , New York: McGraw-Hill; 
Slosarczyk, A., Bia Oskorski, J., Hardness and fracture toughness of dense calcium-phosphate based materials (1998) J Mater Sci Mater Med, 9, pp. 103-108
},
	month = {1999///},
	pages = {2085 - 2090},
	publisher = {Elsevier Science Ltd},
	abstract = {Fracture toughness and hardness properties of CaO-P                        2O
                        5 glass-reinforced hydroxyapatite composites have been assessed using indentation techniques and results calculated according to Laugier and Evans{\textquoteright} equations. Both properties showed to be dependent upon several microstructural characteristics, namely residual porosity and the percentage of secondary β and α tricalcium phosphate phases in the structure of the composites. Composites presented a Palmqvist-type indentation crack system, which is the specific crack system addressed by Laugier{\textquoteright}s approach. Fracture toughness determinations according to Evan{\textquoteright}s equation, which is a universal one and adapted to both median and Palmqvist crack systems, did not correlate well with Laugier determinations. Copyright (C) 1999 Elsevier Science Ltd.
Fracture toughness and hardness properties of CaO-P
                        2O
                        5 glass-reinforced hydroxyapatite composites have been assessed using indentation techniques and results calculated according to Laugier and Evans{\textquoteright} equations. Both properties showed to be dependent upon several microstructural characteristics, namely residual porosity and the percentage of secondary β and α tricalcium phosphate phases in the structure of the composites. Composites presented a Palmqvist-type indentation crack system, which is the specific crack system addressed by Laugier{\textquoteright}s approach. Fracture toughness determinations according to Evan{\textquoteright}s equation, which is a universal one and adapted to both median and Palmqvist crack systems, did not correlate well with Laugier determinations.
},
	keywords = {article, Biocompatible Materials, Biomaterials, calcium phosphate, Calcium phosphates, ceramics, Composite materials, Composite Resins, Cracks, Durapatite, Evan{\textquoteright}s equation, Fracture toughness, glass, hardness, hydroxyapatite, Hydroxyapatite-glass composites, Indentation techniques, Kinetics, Laugier equations, material state, mechanical stress, Microscopy, Electron, Scanning, Microstructure, Palmqvist crack systems, Phosphate minerals, porosity, priority journal, Stress, Mechanical, tensile strength, Tricalcium phosphate},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033231746\&partnerID=40\&md5=55066a0fcfb28ee10321c5820371f75a},
	author = {Lopes, M.A. and Monteiro, F.J. and Santos, J.D.}
}
@article {2572,
	title = {Glass-reinforced hydroxyapatite composites: Secondary phase proportions and densification effects on biaxial bending strength},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {48},
	year = {1999},
	note = {Cited By (since 1996): 28Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(1999)48:5<734::AID-JBM20>3.0.CO;2-2PubMed ID: 10490690Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB-Instituto Engenharia Biomedica, Laboratorio de Biomateriais, Rua do Campo Alegre, 823, 4150 Porto, PortugalChemicals/CAS: alpha-tricalcium phosphate; Biocompatible Materials; calcium phosphate, 10103-46-5; Calcium Phosphates; dicalcium phosphate anhydrous, 7757-93-9; Hydroxyapatites; monocalcium phosphate; tetracalcium phosphateReferences: Yamamuro, Y., Hench, L.L., Wilson, J., (1990) CRC Handbook of Bioactive Ceramics, , Boca Raton, FL: CRC;Rice, R.W., Microstructure dependence of mechanical behaviour (1977) Treatise on Materials Science and Technology, 11. , MacCrone RK, editor. New York: Academic; 
Rice, R.W., Grain size and porosity dependence of ceramic fracture energy and toughness at 22{\textdegree}C (1996) J Mat Sci, 31, pp. 1969-1983; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterisation of glass reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Wayne, S.F., Buljan, S.T., Microstructure and wear resistance of silicon nitride composites (1994) Friction and Wear of Ceramics, p. 261. , Jahanmir S, editor. New York: Marcel Dekker; 
Kaysser, W.A., Sprissler, M., Handwerker, C.A., Blendell, J.E., Effect of liquid phase on the morphology of grain growth of alumina (1987) J Am Ceram Soc, 70, pp. 339-343; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Soltesz, U., Ritcher, H., Kienzler, R., The concentric-ring test and its applications for determining the surface strength of ceramics (1987) High Tech Ceram, pp. 149-159. , Amsterdam: Elsevier; 
Murtage, B., (1981) Advance Linear Programming, , New York: McGraw-Hill; 
Cha{\textquoteright}tal, V., (1990) Linear Programming, , New York: Freeman; 
Davidge, R.W., (1986) Mechanical Behaviour of Ceramics, , Cambridge: Cambridge Univ Press; 
German, R.M., (1985) Liquid Phase Sintering, , New York: Plenum; 
Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812; 
Fanovich, M.A., Lopes, J.M.P., Influence of temperature and additives on the microstructure and sintering behaviour of hydroxyapatites with different Ca/P ratios (1998) J Mat Sci Mat Med, 9, pp. 53-60; 
Jarcho, M., Bolen, C.H., Bobik, J., Kay, J.F., Doremus, R.H., Hydroxyapatite synthesis and characterisation in dense polycrystalline form (1976) J Mat Sci, 11, pp. 2027-2035; 
Van Landuyt, P., Li, F., Keustermans, J.P., Streydio, J.M., Delnnay, F., Munting, E., The influence of high sintering temperatures on the mechanical properties of hydroxyapatite (1995) J Mat Sci Mat Med, 6, pp. 8-13; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , Japanese Association of Apatite Layer; 
Chawla, K.K., (1993) Ceramic Matrix Composites, , London, UK: Chapman Hall; 
Barsoum, M., Fundamentals of Ceramics, , New York: McGraw-Hill; 1997
},
	month = {1999///},
	pages = {734 - 740},
	publisher = {John Wiley \& Sons Inc},
	abstract = {CaO-P                        2O
                        5 glasses with additions of MgO and CaF
                        2 were used as a sintering aid of hydroxyapatite, and glass-reinforced hydroxyapatite composites obtained. Glasses promoted significant changes in the microstructure of the composites, namely with the formation of tricalcium phosphate secondary phases, β and α-TCP. Quantitative phase analysis was performed by the Rietveld method using General Structure Analysis Software. Grain size measurements were carried out on SEM photomicrographs, using a planimetric procedure according to ASTM E 112-88. Flexural bending strength was determined from concentric ring-on-ring testing. Flexural bending strength (FBS) of glass-reinforced hydroxyapatite composites was found to be about twice or three times higher than that of unreinforced hydroxyapatite and tended to depend more on porosity and β and α-TCP secondary phases, rather than on grain size. Traces of α-tricalcium phosphate significantly enhanced the strength of the composites. Using the rule of mixtures to estimate the zero porosity bending strength, the Duckworth-Knudsen model applied to the composites gave a porosity correction factor, b, with a value of 4.02. Weibull statistics were also used to analyze biaxial strength data and the level of reinforcement obtained by comparing failure probability for the composites and for the unreinforced hydroxyapatite. Lower activation energies for grain growth were observed for the composites compared to unreinforced hydroxyapatite, which should be attributed to the presence of a liquid glassy phase that promotes atomic diffusion during the sintering process.
CaO-P
                        2O
                        5 glasses with additions of MgO and CaF
                        2 were used as a sintering aid of hydroxyapatite, and glass-reinforced hydroxyapatite composites obtained. Glasses promoted significant changes in the microstructure of the composites, namely with the formation of tricalcium phosphate secondary phases, β and α-TCP. Quantitative phase analysis was performed by the Rietveld method using General Structure Analysis Software. Grain size measurements were carried out on SEM photomicrographs, using a planimetric procedure according to ASTM E 112-88. Flexural bending strength was determined from concentric ring-on-ring testing. Flexural bending strength (FBS) of glass-reinforced hydroxyapatite composites was found to be about twice or three times higher than that of unreinforced hydroxyapatite and tended to depend more on porosity and β and α-TCP secondary phases, rather than on grain size. Traces of α-tricalcium phosphate significantly enhanced the strength of the composites. Using the rule of mixtures to estimate the zero porosity bending strength, the Duckworth-Knudsen model applied to the composites gave a porosity correction factor, b, with a value of 4.02. Weibull statistics were also used to analyze biaxial strength data and the level of reinforcement obtained by comparing failure probability for the composites and for the unreinforced hydroxyapatite. Lower activation energies for grain growth were observed for the composites compared to unreinforced hydroxyapatite, which should be attributed to the presence of a liquid glassy phase that promotes atomic diffusion during the sintering process.
},
	keywords = {article, atom, Bending (deformation), Biocompatibility, Biocompatible Materials, Biomaterials, calcium fluoride, calcium phosphate, Calcium phosphates, Composite materials, Densification, Dental application, dentistry, Duckworth-Knudsen model, glass, Glass reinforced hydroxyapatite, Humans, hydroxyapatite, Hydroxyapatite-glass composites, Hydroxyapatites, Implants (surgical), Magnesium Oxide, mathematical analysis, Mechanical strength, Mechanics, Medical applications, Microstructure, orthopedics, Phase composition, physical chemistry, porosity, Scanning electron microscopy, Strength of materials, tensile strength, Tissue, Tricalcium phosphate},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032868650\&partnerID=40\&md5=e6fa831a838301db0336e9010a0800dd},
	author = {Lopes, M.A. and Monteiro, F.J. and Santos, J.D.}
}
@article {2575,
	title = {Hydrophobicity, surface tension, and zeta potential measurements of glass-reinforced hydroxyapatite composites},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {45},
	year = {1999},
	note = {Cited By (since 1996): 46Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19990615)45:4<370::AID-JBM12>3.0.CO;2-0PubMed ID: 10321710Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB, University of Porto, Rua do Campo Alegre, 823, 4150 Porto, PortugalChemicals/CAS: Biocompatible Materials; Calcium Phosphates; Hydroxyapatites; Proteins; VitronectinReferences: Yamamuro, Y., Hench, L.L., Wilson, J., (1990) CRC Handbook of Bioactive Ceramics, , Boca Raton, FL: CRC Press;Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterization of glass reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Aoki, H., Medical applications of hydroxyapatite (1991) Science and Medical Applications of Hydroxyapatite, pp. 1-214. , Aoki H, Sawai K, editors. Tokyo: Takyama Press System Center; 
Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812; 
Davies, J.E., The importance and measurement of surface charge species in cell behaviour at the biomaterial interface (1988) Surface Characterization of Biomaterials, pp. 219-234. , Ratner BD. New York: Elsevier; 
Ratner, B.D., Biomaterial surfaces (1987) J Biomed Mater Res Appl Biomat, 21, pp. 59-90; 
Manson, S.R., Harker, L.A., Ratner, B.D., Hoffman, A.S., Evaluation of artificial surfaces with a non human primate model of arterial thrombosis (1980) J Lab Clin Med, 95, pp. 289-304; 
Grinnell, F., Milamand, M., Srere, P.A., Studies on cell adhesion (1972) Arch biochem biophys, 153, pp. 193-198; 
Chang, S.K., Hum, O.S., Moscarello, Ma., Neumann, A.W., Zing, W., Leutheusser, M.J., Ruegsegger, B., Platelet adhesion to solid surfaces: The effect of plasma proteins and substrate wettability (1997) Med Progr Technol, 5, pp. 57-66; 
Agathopoulos, S., Nikolopoulos, P., Determination of the energy of the interactions oxides and biological liquids (1995) Fourth Euro Ceramics, 8, pp. 319-324. , Ravaglioli A, editor; 
Lapin, M., Warocquier-Cl{\'e}rout, R., Legris, C., Degrange, M., Luizard, M.F.S., Correlation between roughness and wettability, cell adhesion and cell migration (1997) J Biomed Mater Res, 36, pp. 99-108; 
Williams, D.F., Bagnall, R.D., Adsorption of proteins on polymers and its role in the response of soft tissue (1998) Fundamental Aspects of Biocompatibility, 2, pp. 113-127. , Williams DF, editor. Boca Raton, FL: CRC Press; 
Dimitrov, S., Krachevsky, P.A., Nikolov, A.D., Noshi, H., Matsumoto, M., Contact angle measurements with sessile drops and bubbles (1991) J Colloid Interface Sci, 145, pp. 279-282; 
Adamson, W., (1990) Physical chemistry of surfaces. 5th edition, pp. 4-52. , New York: Wiley; 
Young, T., (1835) Miscellaneous Works, 1, p. 418. , Peacock G, editor London: Murray; 
Young, T., (1805) Philos Trans R Soc Lond, 95, p. 65; 
Correia, N.T., Ramos, J.M., Saramago, B., Calado, J., Estimation of the surface tension of a solid: Application to a liquid crystalline (1997) J Colloid Interface Sci, 189, pp. 361-369; 
Owens, D.K., Wendt, R.D., Estimation of the surface free energy of polymers (1969) J Appl Polym Sci, 13, p. 1741; 
Schrader, M.E., Young dupr{\'e} revisited (1995) Langmuir, 11, p. 3585; 
Kowalchuk, R.M., Pollack, S.R., Ducheyne, P., King, L.A., Particle microelectrophoresis of calcium-deficient hydroxyapatite: Solution composition and kinetics effects (1993) J Biomed Mater Res, 27, pp. 783-790; 
Ducheyne, P., Kim, C.S., Pollack, S.R., The effect of phase differences on the time dependent variation of the zeta potential of hydroxyapatite (1992) J Biomed Mater Res, 26, pp. 147-168; 
Suzuki, T., Nishizawa, K., Yokogawa, Y., Kameyama, T., Time-dependent variation of the surface structure of bioceramics in tissue culture medium and the effect on adhesiveness of cells (1996) J Ferment Bioeng, 81, pp. 226-232; 
Susuki, T., Yamamoto, T., Yoriyama, M., Kameyama, T., Surface instability of calcium phosphates ceramics in tissue culture medium and the effect on adhesion and growth of anchorage-dependent animal cells (1997) J Biomed Mater Res, 34, pp. 507-517; 
Hay, H., (1991) Cell Biology of Extracellular Matrix. 2nd Edition, pp. 127-129. , New York: Plenum Press; 
Seiffert, D., Detection of vitronectin in mineralized bone matrix (1986) J Histochem Cytochem, 44, pp. 275-280; 
Rodan, G.A., Introduction to bone biology (1992) Bone, 12, p. 53; 
Young, M.E., Kerr, J.M., Ibaraki, K., Heegaard, A.M., Gehron, R.P., Structure, expression and regulation of the major matrix proteins of bone (1992) Clin Orthop Rel Res, 281, p. 275; 
Formigli, L., Zecchi, S., Benvenuti, S., Brandi, M.L., Cell to cell interaction on bone tissue (1992) Ann NY Acad Sci, 673, p. 120; 
Underwound, P.A., Bennett, F.A., A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin (1989) J Cell Sci, 93, pp. 641-649; 
Hayman, E.G., Pierschbacher, M.D., Suzuki, S., Ruoslahti, E., Vitronectin: A major cell attachment-promoting protein in foetal bovine serum (1985) Exp Cell Res, 160, pp. 245-250; 
Lopes, Ma., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Lopes, Ma., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Cheng, P., Li, D., Boruvka, L., Rotenberg, Y., Neumann, A.W., Automation of axisymmetric drop shape analysis for measurements of interfacial tensions and contact angles (1990) Colloids Surf, 43, pp. 151-167; 
Cheng, P., Neumann, A.W., Computational evaluation of axisymmetric drop shape analysis-profile (ADSA-P) (1992) Colloids Surf, 62, pp. 297-306; 
Saramago, B., Teixeira, M.J., Rosa, M.E., Bordadao, J.C., Influence of the wetting properties of polymeric adhesives on the mechanical behaviour of cork agglomerates (1996) A Adhes Sci Technol, 10, pp. 1111-1127; 
Voigt, A., Thiel, O., Williams, D., Policova, Z., Zingg, W., Neumann, A.W., Asymmetric drop shape analysis (ADSA) applied to protein solution (1991) Colloid Surf, 58, p. 315; 
Williams, R.L., Hunt, J.A., Tengvall, P., Fibroblast adhesion onto methyl-silica gradients with and without preadsorbed proteins (1995) J Biomed Mater Res, 29, pp. 1545-1555; 
Hench, L.L., Ethridge, E.C., (1982) Biomaterials: An Interfacial Approach, , New York: Academic Press; 
Horbert, T.A., Brash, J.L., Proteins at interfaces. II. Fundamentals and applications (1994) ACS Symposium, 602, pp. 1-23. , Chapter 1, Chapter 7; 
Vogler, E.A., Interfacial chemistry in biomaterials science (1993) Wettability, 49, pp. 183-250. , Berg JC, editor New York: Marcel Dekker; Chapter 4
},
	month = {1999///},
	pages = {370 - 375},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Wettability and zeta potential studies were performed to characterize the hydrophobicity, surface tension, and surface charge of P                        2O
                        5-glass- reinforced hydroxyapatite composites. Quantitative phase analysis was performed by the Rietveld method using GSAS software applied to X-ray diffractograms. Surface charge was assessed by zeta potential measurements. Protein adsorption studies were performed using vitronectin. Contact angles and surface tensions variation with time were determined by the sessile and pendent drop techniques, respectively, using ADSA-P software. The highest (- 18.1 mV) and lowest (-28.7 mV) values of zeta potential were found for hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), respectively, with composite materials presenting values in between. All studied bioceramic materials showed similar solid surface tension. For HA and β-TCP, solid surface tensions of 46.7 and 45.3 mJ/m
                        2, respectively, were obtained, while composites presented intermediate surface tension values. The dispersive component of surface tension was the predominant one for all materials studied. Adhesion work values between the vitronectin solution and HA and β- TCP were found to be 79.8 and 88.0 mJ/m
                        2, respectively, while the 4.0 wt \% glass composites showed slightly lower values than the 2.5 wt \% ones. The presence of β-TCP influenced surface charge, hydrophobicity, and protein adsorption of the glass-reinforced HA composites, and therefore indirectly affected cell-biomaterial interactions.
The hydrophobicity, surface tension, and surface charge of P
                        2O
                        5-glass-reinforced hydroxyapatite (HA) composites were characterized through wettability and zeta potential studies. The highest and lowest values of zeta potential were found for HA and β-TCP. All studied bioceramic materials showed similar solid surface tension. The presence of β-TCP indirectly affected cell-biomaterial interactions.
},
	keywords = {Adhesion, Adsorption, Algorithms, article, Biocompatible Materials, biomaterial, Biomaterials, Bone, Calcium Compounds, calcium phosphate, Calcium phosphates, Cells, Chemical analysis, Chemistry, Physical, Composite materials, Contact angle, glass, Glass reinforced hydroxyapatite composites, hydrophobicity, hydroxyapatite, Hydroxyapatite-glass composites, Hydroxyapatites, implant, Implants (surgical), Interfacial energy, Phosphorus Compounds, Protein adsorption, Proteins, Surface charge, Surface Properties, surface property, Surface tension, Tricalcium phosphate, Vitronectin, wettability, X-Ray Diffraction, Zeta potential},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033563979\&partnerID=40\&md5=3807a20c608ec3dcf8cce1d48213c1a3},
	author = {Lopes, M.A. and Monteiro, F.J. and Santos, J.D. and Serro, A.P. and Saramago, B.}
}
@article {2573,
	title = {In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {10},
	year = {1999},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1008924516146Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Institute of Biomedical Engineering, Faculties of Dental Medicine, Universidade do Porto, 4200 Porto, PortugalChemicals/CAS: alkaline phosphatase, 9001-78-9; calcium ion, 14127-61-8; hydroxyapatite, 1306-06-5, 51198-94-8; phosphorus, 7723-14-0; protein, 67254-75-5References: Passuti, N., Daculsi, G., Martin, S., (1990) Clinical Implant Materials, p. 255. , edited by V. Soltesz and A. Lee (Elsevier, Amsterdam);Legeros, R., (1988) Adv. Dental Res., 2, p. 164; 
Hench, L.L., (1992) J. Amer. Ceram. Soc., 74, p. 1487; 
Kokubo, T., (1993) Bone-Bonding Biomaterals, p. 31. , edited by P. Ducheyne, T. Kokubo and C. A. Blitterswijk (Reed Health-Care Comunications, Leiden, The Netherlands); 
Daculsi, G., Legeros, R.Z., Henghebaert, M., Barbieux, I., (1990) Calcif. Tissue Int., 46, p. 20; 
Bagambisa, F.B., Joos, U., Schilli, W., (1993) J. Biomed. Mater. Res., 27, p. 1047; 
De Bruijn, J.D., Van Blitterswijk, C.A., Davies, J.E., (1995) J. Biomed. Mater. Res., 29, p. 89; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , JAAS; 
Raemdonck, W., Ducheyne, P., Meester, P., (1984) Metal and Ceramic Materials, p. 143. , edited by P. Ducheyne and G. W. Hastings (CRC Press, Boca Raton, FL); 
Rey, C., Freche, M., Heughebaert, M., Vignoles, M., (1991) Bioceramics 4, p. 57. , edited by W. Bonfield, G. W. Hastings and K. E. Tanner (Butterworth-Heinemann, London); 
Nagase, M., Abe, Y., Udagawa, E., (1992) Biomaterials, 13, p. 172; 
Burnie, J., Gilchrist, T., (1993) Ceramics in Surgery, p. 169. , edited by P. Vincenzini (Elsevier, Amsterdam); 
Burnie, F., Gilchrist, T., Ceramics in Surgery, p. 177; 
Rawlings, R.D., Rogers, P.S., Stokes, P.M., (1987) High Tech Ceramics, p. 73. , edited by P. Vincenzini (Elsevier, Amsterdam); 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Santos, J.D., Lakhan, J.J., Monteiro, F.J., (1995) Biomaterials, 16, p. 521; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., (1995) J. Mater. Sci. Mater. Med., 6, p. 348; 
Klein, C.P.A.T., Patka, P., Van Der Lubbe, H.B.M., (1991) J. Biomed. Mater. Res., 25, p. 53; 
Jansen, J.A., Van Der Waerden, J.P.C.M., Wolke, J.G.C., (1993) J. Mater. Sci. Mater. Med., 4, p. 466; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., (1992) J. Biomed. Mater. Res., 26, p. 1365; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., (1994) J. Mater. Sci. Mater. Med., 5, p. 11; 
Lugscheider, E., Knepper, M., Heimberg, B., Dekker, A., Kirkpatrick, C.J., (1994) J. Mater. Sci. Mater. Med., 5, p. 371; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., (1995) Biomaterials, 16, p. 489; 
Labat, B., Chanson, A., Frey, J., (1995) J. Biomed. Mater. Res., 29, p. 1397; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., (1996) J. Mater. Sci. Mater. Med., 7, p. 21; 
Anselme, K., Sharrock, P., Hardouin, P., Dard, M., (1997) J. Biomed. Mater. Res., 34, p. 247; 
Ong, J.L., Prince, C.W., Lucas, L.C., (1995) J. Biomed. Mater. Res., 29, p. 165; 
Vrouwenvelder, W.C.A., Groot, C.G., De Groot, K., (1995) J. Mater. Sci. Mater. Med., 6, p. 144; 
Am{\'e}d{\'e}e, J., Bareille, R., Jeandot, R., Bordenave, L., R{\'e}my, M., Rouais, F., Baquey, C., (1994) Biomaterials, 15, p. 1029; 
Maniatopoulos, C., Sodek, J., Melcher, A., (1988) Cell Tissue Res., 254, p. 317; 
Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., (1990) J. Cell Phys., 143, p. 213; 
Gundle, R., Joyner, C.J., Triffit, J.T., (1995) Bone, 16, p. 597; 
Fernandes, M.H., Costa, M.A., Carvalho, G.S., (1997) J. Mater. Sci. Mater. Med., 8, p. 61; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., (1997) J. Mater. Sci. Mater. Med., 8, p. 233; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., (1996) J. Mater. Sci. Mater. Med., 7, p. 291; 
Morais, S., Dias, N., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., (1999) J. Biomed. Mater. Res., 44, p. 176; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., (1991) Bone and Min., 14, p. 27; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., J. Biomed. Mater. Res., , submitted; 
Owen, T.A., Aronow, M., Shalhoub, V., (1990) J. Cell Phys., 143, p. 420; 
Martin, T.J., Findlay, D.M., Heath, J.K., Ng, K.W., (1993) Handbook of Experimental Pharmacology, p. 149. , edited by J. R. Mundy and T. J. Martin Berlin (Springer-Verlag); 
Stein, G.S., Lian, J.B., (1993) Cellular and Molecular Biology of Bone, p. 47. , edited by M. Noda (Academic Press, Tokyo); 
Anderson, H.C., Morris, D.C., (1993) Handbook of Experimental Pharmacology, p. 267. , edited by J. R. Mundy and T. J. Martin Berlin (Springer-Verlag); 
Gwynn, I.A.P., (1994) J. Mater. Sci. Mater. Med., 5, p. 357; 
Kirkpatrick, C.J., Wagner, M., Kohler, H., Bittinger, F., Otto, M., Klein, C.L., (1997) J. Mater. Sci. Mater. Med., 8, p. 131; 
Legeros, R.Z., Orly, I., Gregoire, M., Daculsi, G., (1991) The Bone-Biomaterial Interface, p. 76. , edited by J. E. Davies (University of Toronto Press, Toronto); 
Chern Lin, J.H., Liu, M.L., Ju, C.P., (1994) J. Biomed. Mater. Res., 28, p. 723; 
Ghannam, E.L., Ducheyne, P., Spapiro, I., (1995) J. Biomed. Mater. Res., 29, p. 359; 
Radin, S.R., Ducheyne, P., (1993) J. Biomed. Mater. Res., 27, p. 35; 
Radin, S.R., Ducheyne, P., (1993) J. Biomed. Mater. Res., 27, p. 1047; 
Best, S., Sim, B., Kayser, M., Downes, S., (1997) J. Mater. Sci. Mater. Med., 8, p. 97
},
	month = {1999///},
	pages = {567 - 576},
	publisher = {Kluwer Academic Publishers},
	abstract = {Human osteoblastic bone marrow cells were cultured far periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the {\textquoteright}as-received{\textquoteright} condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorus in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the {\textquoteright}as-received{\textquoteright} HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, {\textquoteright}as-received{\textquoteright} HA hardly supported cell growth. Previously immersed material samples showed cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow eel Is was closer to that observed in control cultures.Human osteoblastic bone marrow cells were cultured for periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the {\textquoteright}as-received{\textquoteright} condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorous in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the {\textquoteright}as-received{\textquoteright} HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, {\textquoteright}as-received{\textquoteright} HA hardly supported cell growth. Previously immersed material samples cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow cells was closer to that observed in control cultures.
},
	keywords = {adult, alkaline phosphatase, article, Bone, bone marrow cell, Bone replacement materials, Calcium, calcium ion, case report, Cell culture, cell differentiation, cell growth, Cells, controlled study, culture medium, enzyme activity, Enzyme kinetics, glass, Growth kinetics, human, Human bone marrow cell, human cell, hydroxyapatite, male, osteoblast, Phosphates, phosphorus, priority journal, protein, protein determination, Proteins, scanning electron microscope, Scanning electron microscopy, Sprayed coatings},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033198788\&partnerID=40\&md5=ed28cf28c5da19665b6a3c3c916dad56},
	author = {Ferraz, M.P. and Fernandes, M.H. and Cabral, A.T. and Santos, J.D. and Monteiro, F.J.}
}
@article {2577,
	title = {In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {44},
	year = {1999},
	note = {Cited By (since 1996): 35Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(199902)44:2<176::AID-JBM8>3.0.CO;2-6PubMed ID: 10397919Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Universidade do Porto, Rua Dr Manuel Pereira da Silva, 4200 Porto, PortugalChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; Alloys; Biocompatible Materials; Calcium, 7440-70-2; Chromium, 7440-47-3; Iron, 7439-89-6; Metals; Nickel, 7440-02-0; Phosphorus, 7723-14-0; Proteins; Stainless Steel, 12597-68-1; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1References: Williams, D., Cahn, R.W., Bever, M.B., (1990) Concise Encyclopedia of Medical \& Dental Materials, pp. 4-70. , Oxford, New York, Beijing, Frankfurt, S{\~a}o Paulo, Sydney, Tokyo, Toronto: Permagon Press;Linden, J.V., Hopler, S.M., Gossling, H.R., Sunderman Jr., F.W., Blood nickel concentrations in patients with stainless-steel hip protheses (1985) Ann Clin Lab Sci, 15, pp. 459-464. , 1985; 
Weber, G.W., Reid, B.L., Nickel toxicity in young growing mice (1969) J Animal Sci, 28, pp. 620-623; 
O{\textquoteright}Dell, G.D., Miller, W.J., King, W.A., Moore, S.L., Blackmon, D.M., Nickel toxicity in the young bovine (1970) J Nutrition, 100, pp. 1447-1453; 
Louria, D.B., Joselow, M.M., Browder, A.A., The human toxicity of certain trace elements (1972) Ann Intern Med, 76, pp. 307-319; 
Whanger, P.D., Effects of dietary nickel on enzyme activities and mineral contents in rats (1973) Toxicol Appl Pharmacol, 25, pp. 323-331; 
Woodman, J.L., Black, J., Jimenez, S., Organometallic corrosion products of 316L SS and HS-21 in serum: In vitro and in vivo comparison in an acute rat model (1982) Biomaterials 1980, pp. 245-253. , Winter GD, Gibbon DF, Plenk Jr H, editors. Chichester, New York, Brisbone, Toronto, Singapore: John Wiley \& Sons; 
Parfitt, A.M., The physiological and clinical significance of bone histomorphometric data (1983) Bone Histomorphometry: Techniques and Interpretation, pp. 143-223. , Recker RR, editor. Boca Raton, Florida: CRC Press; 
Jilka, R.L., Manolagas, S.C., The cellular and biochemical basis of bone remodeling (1994) Osteoporosis, pp. 17-48. , Marcus R, editor. Boston: Blackwell Scientific Publications; 
Owen, M., Lineage of osteogenic cells and their relationship to the stromal system (1985) Bone and Mineral Research, 3, pp. 1-25. , Peck WA, editor. Amsterdam: Elsevier Science Publishers; 
Owen, T.A., Aronow, M., Shalhoub, V., Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix (1990) J Cell Physiol, 143, pp. 420-430; 
Morais, S., Carvalho, G.S., Sousa, J.P., Potentiometric determination of total and ionized Ca in osteoblast-like cell culture medium (1996) Electroanalysis, 8 (12), pp. 1174-1178; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9 (10), pp. 791-795; 
Morais, S., Carvalho, G.S., Sousa, J.P., Chromium determination in osteoblast-like cell culture medium by catalytic cathodic stripping voltammetry with a mercury microelectrode (1998) J Trace Elements Med Biol, 2, pp. 101-108; 
Morais, S., Carvalho, G.S., Sousa, J.P., Determination of Ni in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with mercury microelectrodes (1997) Electroanalysis, 9 (5), pp. 422-426; 
Morais, S., Carvalho, G.S., Sousa, J.P., Optimization study for nickel quantification in cell culture medium using mercury film microelectrodes (1996) Portugaliae Electrochimica Acta, 14, pp. 267-272; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tiss Res, 254, pp. 317-330; 
Berridge, V., Tan, A.S., Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction (1993) Arch Biochem Biophys, 303, pp. 474-482; 
Bessey, O., Lowry, O., Breck, M., A method for the rapid determination of alkaline phosphatase with five cubic millileters of serum (1946) J Biol Chem, 164, pp. 321-326; 
McGee-Russell, M., Histochemical methods for calcium (1958) J Histochem Cytochem, 6, pp. 22-42; 
Fiske, C.H., Subbarow, Y., The colorimetric determination of phosphorus (1925) J Biol Chem, 66, pp. 373-400; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells (1990) J Cell Phys, 143, pp. 213-221; 
Gotoh, Y., Hiraiwa, K., Narajama, M., In vitro mineralization of osteoblastic cells derived from human bone (1990) Bone and Miner, 8, pp. 239-250; 
Cheng, S.L., Yang, J.W., Riffas, L., Zhang, S.F., Avioli, L.V., Differentiation of human bone marrow osteogenic stromal cells in vitro. Induction of the osteoblast phenotype by dexamethasone (1994) Endocrinology, 134, pp. 277-286; 
Gundle, R., Joyner, C.J., Triffit, J.T., Human bone tissue formation in diffusion chamber culture in vivo by bone-derived cells and marrow stromal fibroblastic cells (1995) Bone, 16, pp. 597-601; 
Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Arcicla, C.R., Granchi, D.C., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin Mater, 15, pp. 173-190; 
Gerstenfeld, L.C., Chipman, S.D., Glowacki, J., Lian, J.B., Expression of differential function by mineralizing cultures of chicken osteoblasts (1987) Dev Biol, 122, pp. 46-60; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Initiation and progression of mineralization of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphate (1991) Bone and Miner, 14, pp. 27-40; 
Bellows, C.G., Heersche, J.N.M., Aubin, J.E., Inorganic phosphate added exogenously or released from β-glycerophosphate initiates mineralization of osteoid nodules in vitro (1992) Bone and Miner, 17, pp. 15-29; 
Chung, C.H., Golub, E.E., Forbes, E., Tokuoka, T., Shapiro, I.M., Mechanisms of action of β-glycerophosphate on bone cell mineralization (1992) Calcif Tissue Int, 51, pp. 305-311; 
Boskey, A.L., Stiner, D., Doty, S.B., Binderman, I., Levoy, P., Studies of mineralization in tissue culture: Optimal conditions for cartilage calcification (1992) Bone and Miner, 16, pp. 11-36; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., De Bruijn, J.D., Van Blitterswijk, C.A., Decreased consumption of Ca and P during in vitro biomineralization and biologically induced deposition of Ni and Cr in presence of stainless steel corrosion products (1998) J Biomed Mater Res, 42, pp. 199-212; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralization by osteoblast-like cells. 1. Retardation of tissue mineralization by metal salts (1998) Biomaterials, 19, pp. 13-21; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., De Bruijn, J.D., Van Blitterswijk, C.A., Effects of AISI 316L corrosion products in in vitro bone formation (1998) Biomaterials, 19, pp. 999-1007; 
Stein, G.S., Lian, J.B., Molecular mechanisms mediating developmental and hormone-regulated expression of genes in osteoblasts: An integrated relationship of cell growth and differentiation (1993) Cellular and Molecular Biology of Bone, pp. 47-95. , Noda M, editor. Tokyo: Academic Press Inc; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., The ultrastructure of the one-hydroxyapatite interface in vitro (1992) J Biomed Mater Res, 26, pp. 1365-1382; 
Stein, G.S., Lion, J.B., Molecular mechanisms mediated proliferation-differentiation interrelationships during progressive development of the osteoblast phenotype: Update 1995 (1995) Endocrin Rev, 4, pp. 290-297; 
Abe, Y., Akamine, A., Aida, Y., Maeda, K., Differentiation and mineralization in osteogenic precursor cells derived from fetal rat mandibular bone (1993) Calcif Tissue Int, 52, pp. 365-371; 
Lomri, A., Tran, M.P.V., Mott, M., Characterization of endosteal osteoblastic cells isolated from mouse caudal vertebrae (1988) Bone, 9, pp. 165-175; 
McCulloch, C.A.G., Fair, C.A., Tenebaum, H.C., Limeback, H., Homareau, R., Clonal distribution of osteoprogenitor cells in cultured chick peristoa: Functional relationship to bone formation (1990) Dev Biol, 140, pp. 352-361; 
Sun, Z.L., Wataha, J.C., Hanks, C.T., Effects of metal ions on osteoblast-like cell metabolism and differentiation (1997) J Biomed Mater Res, 34, pp. 29-37; 
Puleo, D.A., Huh, W.W., Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 109-116; 
Katz, S.A., Salem, H., The toxicology of Cr with respect to its chemical speciation: A review (1993) J Appl Toxicol, 13, pp. 217-224; 
Anderson, H.C., Morris, D.C., Mineralization (1993) Handbook of Experimental Pharmacology, pp. 267-298. , Mundy GR, Martin TJ, editors. Berlin: Springer-Verlag; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , Fraker AC, Griffin CD, editors. ASTM STP 859. Philadelphia: American Society for Testing and Materials; 
Rae, T., The toxicity of metals used in orthopaedic prostheses (1981) J Bone Joint Surg, 63 B, pp. 435-440; 
Webb, M., Weinzier, S.M., Uptake of 63Ni 2+ from its complexes with proteins and other ligands by mouse dermal fibroblasts in vitro (1972) Br J Cancer, 26, pp. 292-298; 
Merritt, K., Wortman, R.S., Millard, M., Brown, S.A., XPS analysis of 316LVM corroded in serum and saline (1983) Biomater Med Devices Artif Organs, 11, pp. 115-124; 
Bianchi, V., Toso, R.D., Debetto, P., Mechanisms of chromium toxicity in mammalian cell cultures (1980) Toxicology, 17, pp. 219-224; 
Lucas, L.C., Bearden, L.J., Lemons, J.E., Ultrastructural examination of in vitro and in vivo cells exposed to elements from type 316L stainless steel (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 208-222. , Fraker AC, Griffin CD, editors. ASTM STP 859. Philadelphia: American Society for Testing and Materials
},
	month = {1999///},
	pages = {176 - 190},
	publisher = {John Wiley \& Sons Inc},
	abstract = {For periods up to 21 days human bone marrow was cultured in control conditions that favor the proliferation and differentiation of osteoblastic cells. The effect of AISI 316L corrosion products and the corresponding major separate metal ions (Fe, Cr, and Ni) were studied in three different phases of the culture period in order to investigate the effects of metal ions in cell populations representative of osteoblastic cells in different stages of differentiation. Toxicity consequences of the presence of metal ions in bone marrow cultures were evaluated by biochemical parameters (enzymatic reduction of MTT, alkaline phosphatase activity, and total protein content), histochemical assays (identification of ALP-positive cells and Ca and phosphates deposits), and observation of the cultures by light and scanning electron microscopy. Culture media were analyzed for total and ionized Ca and P and also for metal ions (Fe, Cr, and Ni). The presence of AISI 316L corrosion products and Ni salt in bone marrow cultures during the first and second weeks of culture significantly disturbs the normal behavior of these cultures, interfering in the lag phase and exponential phase of cell growth and ALP expression. However, the presence of these species during the third week of culture, when expression of osteoblastic functions occurs (mineralization process), did not result in any detectable effect. Fe salt also disturbs the behavior of bone marrow cell cultures when present during the lag phase and proliferation phase, and a somewhat compromised response between the normal pattern (control cultures) and intense inhibition (AISI 316L corrosion products and Ni salt-added cultures) was observed. Fe did not affect the progression of the mineralization phase. Osteogenic cultures exposed to Cr salt (Cr                        3+) presented a pattern similar to the controls, indicating that this element does not interfere, in the concentration studied, in the osteoblastic differentiation of bone marrow cells. Quantification of metal ions in the culture media showed that Cr (originated from AISI 316L corrosion products but from not Cr
                        3+ salt) and Ni (originated from AISI 316L corrosion products and Ni salt) appear to be retained by the bone marrow cultures.
For periods up to 21 days human bone marrow was cultured in control conditions that favor the proliferation and differentiation of osteoblastic cells. The effect of AISI 316L corrosion products and the corresponding major separate metal ions (Fe, Cr, and Ni) were studied in three different phases of the culture period in order to investigate the effects of metal ions in cell populations representative of osteoblastic cells in different stages of differentiation. Toxicity consequences of the presence of metal ions in bone marrow cultures were evaluated by biochemical parameters (enzymatic reduction of MTT, alkaline phosphatase activity, and total protein content), histochemical assays (identification of ALP-positive cells and Ca and phosphates deposits), and observation of the cultures by light and scanning electron microscopy. Culture media were analyzed for total and ionized Ca and P and also for metal ions (Fe, Cr, and Ni). The presence of AISI 316L corrosion products and Ni salt in bone marrow cultures during the first and second weeks of culture significantly disturbs the normal behavior of these cultures, interfering in the lag phase and exponential phase of cell growth and ALP expression. However, the presence of these species during the third week of culture, when expression of osteoblastic functions occurs (mineralization process), did not result in any detectable effect. Fe salt also disturbs the behavior of bone marrow cell cultures when present during the lag phase and proliferation phase, and a somewhat compromised response between the normal pattern (control cultures) and intense inhibition (AISI 316L corrosion products and Ni salt-added cultures) was observed. Fe did not affect the progression of the mineralization phase. Osteogenic cultures exposed to Cr salt (Cr
                        3+) presented a pattern similar to the controls, indicating that this element does not interfere, in the concentration studied, in the osteoblastic differentiation of bone marrow cells. Quantification of metal ions in the culture media showed that Cr (originated from AISI 316L corrosion products but from not Cr
                        3+ salt) and Ni (originated from AISI 316L corrosion products and Ni salt) appear to be retained by the bone marrow cultures.
},
	keywords = {alkaline phosphatase, Alloys, article, Batch cell culture, Biocompatible Materials, biotechnology, Bone, Bone cells, bone marrow cell, Bone Marrow Cells, bone mineralization, bone prosthesis, Calcium, Cell culture, cell differentiation, Cell Division, cell population, Cells, Cultured, Chromium, controlled study, Corrosion, Corrosion products, Cytotoxicity, Differentiation, human, human cell, Human osteoblast-like cells, Humans, Implants (surgical), Ions, Iron, joint prosthesis, materials testing, metal ion, Metal ions, Metals, Nickel, osteoblast, Osteoblasts, phosphorus, Prostheses and Implants, Proteins, Stainless steel, Steel corrosion, Tetrazolium Salts, Thiazoles},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033080559\&partnerID=40\&md5=721fda55da342bbc327b233d73789014},
	author = {Morais, S. and Dias, N. and Sousa, J.P. and Fernandes, M.H. and Carvalho, G.S.}
}
@article {2576,
	title = {Miniaturization and application of the MTT assay to evaluate metabolic activity of protozoa in the presence of toxicants},
	journal = {Journal of Basic MicrobiologyJ. Basic Microbiol.},
	volume = {39},
	year = {1999},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JBMIEdoi: 10.1002/(SICI)1521-4028(199905)39:2<103::AID-JOBM103>3.0.CO;2-DPubMed ID: 10335603Language of Original Document: EnglishCorrespondence Address: Lima, N.; Centro de Engenharia Biologica, Universidade do Minho, 4700 Braga, Portugal; email: nelson@iec.uminho.ptChemicals/CAS: Copper, 7440-50-8; Cycloheximide, 66-81-9; Octoxynol, 9002-93-1; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1; Zinc, 7440-66-6References: Berridge, M.V., Tan, S., Characterization of the cellular reduction of using 3-[4,5-dimethyl-thiazol-2-y1]-2,5-diphenyl-tetrazolium bromide-MTT: Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction (1993) Arch. Biochem. Biophysics, 303, pp. 474-482;Berridge, M.V., Tan, S., McCoy, K.D., Wang, R., The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts (1996) Biochemica, 4, pp. 15-20; 
Carvalho, G.S., Castanheira, M., Diogo, I., Abreu, A.M., Sousa, J.P., Loon, J.A., Van Blitterswijk, C.A., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1996) J. Mat. Science: Mat. in Medicine, 7, pp. 77-83; 
Ciappeti, G., Cenni, E., Pratelli, L., Pizzoferrato, A., In vitro evaluation of cell/biomaterial interaction by MTT assay (1993) Biomaterials, 14, pp. 359-364; 
Colgan, D.J., Spectrophotometric quantitation of DNA on blots after ethanol-solubilization of the MTT-formazan from anti-digoxigenin-based detection of nucleic acids (1993) Appl. Theor. Electrophoresis, 3, pp. 219-222; 
Cote, J., Kamen, A.A., Andre, G., Protein-free culture medium improvement. Testing additives and their interactive effects in 96-well plates (1993) Appl. Microb. Biotechnol., 39, pp. 577-584; 
Dias, N., Lima, N., Carvalho, G.S., Mota, M., Miniaturiza{\c c}{\~a}o do ensaio do MTT para avalia{\c c}{\~a}o da actividade metab{\'o}lica dos protozo{\'a}rios (1997) Congresso Nacional de Microbiologia-MICRO{\textquoteright} 97, , Tomar; 
Hanel, T., Gareis, M., Kollarczik, B., Citotoxicity of mycotoxins evaluated by the MTT-cell culture Assay (1994) Mycopathologia, 128, pp. 167-174; 
Lemieux, P., Michaud, M., Pag{\'e}, M., A new formazan amplified clonogenic assay for cytotoxicity testing (1993) Biotechnol. Techniques, 7, pp. 597-602; 
Martin, A., Clynes, C., Comparison of 5 microplate assays for in vitro cytotoxicity testing and cell proliferation assays (1993) Cytotechnology, 11, pp. 49-58; 
Mossman, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J. Immunol. Methods, 65, pp. 55-63; 
Roberts, Ch.T., Orias, E., A cycloheximide-resistant mutant of Tetrahymena pyriformis (1973) Exp. Cell Res., 81, pp. 312-316; 
Sasaki, C.Y., Passaniti, A., Identification of anti-invasive but noncytotoxic chemotherapeutic agents using the tetrazolium dye MTT to quantitative viable cells in matrigel (1998) Bio-techniques, 24, pp. 1038-1043; 
Stowe, R.P., Koenig, D.W., Mishra, S.K., Pierson, D.L., Non destructive and continuous spectrophotometric measurement of cell respiration using a tetrazolium-formazan microemulsion (1995) J. Microbiol. Methods, 22, pp. 283-292; 
Wilson, A.P., Citotoxicity and viability assays (1995) Animal Cell Culture - A Practical Approach. 2nd Ed., pp. 277-289. , (R. I. FRESHNEY, Editor). Oxford University Press, Oxford
},
	month = {1999///},
	pages = {103 - 108},
	abstract = {This paper describes a critical evaluation of a miniaturised colorimetric assay, using MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) reduction, applied to protozoan viability testing. The toxic substances used were copper, zinc, Triton X-100 (a membrane surfactant) and cycloheximide (an inhibitor of the protein synthesis). The viability assay of the ciliate protozoan Tetrahymena pyriformis was optimised in terms of MTT concentration and incubation time. Since protozoa are non adherent cells the MTT assay was modified in order to maintain the medium in the well. MTT proved to be effective in the measurement of Tetrahymena pyriformis viability. Four hours of MTT incubation followed by 30 minutes of incubation with DMSO were found to be the best incubation times for optical density reading. Furthermore, 10 mg/ml of MTT solution was the concentration that gave higher values of optical densities with minor medium interference.},
	keywords = {Animals, Ciliophora, Colorimetry, Copper, Cycloheximide, MTT assay, Octoxynol, Protozoa, Tetrahymena pyriformis, Tetrazolium Salts, Thiazoles, Zinc},
	isbn = {0233111X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032910642\&partnerID=40\&md5=81e222339d4ee5c21619858027047e83},
	author = {Dias, N. and Nicolau, A. and Carvalho, G.S. and Mota, M. and Lima, N.}
}
@article {2586,
	title = {Adhesion and microstructural characterization of plasma-sprayed hydroxyapatite/glass ceramic coatings onto Ti-6Al-4V substrates},
	journal = {Surface and Coatings TechnologySurf. Coat. Technol.},
	volume = {102},
	year = {1998},
	note = {Cited By (since 1996): 41Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Silva, P.L.; INEB-Inst. de Engenharia Biomedica, Pra{\c c}a Coronel Pacheco 1, 4050 Porto, PortugalReferences: Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, p. 165. , Takayama Press System Center, JAAS, Tokyo;Barney, V.C., Levin, M.P., Adams, D.F., (1986) J. Periodont., 57, p. 764; 
Ducheyne, P., (1987) J. Biomed. Mater. Res. Appl. Biomater., 21, p. 219; 
Legeros, R.Z., (1988) Adv. Dent. Res., 2, p. 164; 
Williams, D., (1986) Medical and Dental Materials, , Pergamon Press; 
Akao, M., Haoki, K., Kato, (1981) J. Mater. Sci., 16, p. 809; 
With, G., Van Dijk, H., Hattu, N., Prijs, K., (1981) J. Mater. Sci., 16, p. 1592; 
Santos, J.D., Silva, P.L., Knowles, J.C., Monteiro, F.J., (1996) J. Mater. Sci. Mater. Med., 7, p. 187; 
Rey, C., (1990) Biomateriais, 11, p. 14; 
Evans, G., Behiri, J., Currey, J., Bonfield, B., (1990) J. Mater. Sci. Mater. Med., 1, p. 38; 
Matejka, D., Benko, B., (1989) Plasma Spraying of Metallic Materials, , Wiley, New York; 
Brown, S.D., (1984) Thin Sol. Films, 119, p. 127; 
Mittal, K.L., Adhesion Measurement of Thin Films, Thick Films and Bulk Coatings, , ASTM STP 840; 
ASTM D 1002, standard test method for strength properties of adhesive in shear by tension loading (1983) Annual Book of ASTM Standards; 
ASTM C 633-79, standard test method for adhesion or cohesive strength of flame sprayed coatings (1979) Annual Book of ASTM Standards Section 2; 
Ruiz, J., Gonz{\'a}lez, J.A., (1984) Rev. Metal., 20 (1). , Madrid
},
	month = {1998///},
	pages = {191 - 196},
	abstract = {Commercial hydroxyapatite (HA), Ca                        10(PO
                        4)
                        6(OH)
                        2, powders were reinforced with 4\% of a CaO-P
                        2O
                        5 based glass during its sintering process. Double layered coatings were obtained by Plasma Spraying and composed of 50 μm commercial HA, followed by a 50 μm (HA)/P
                        2O
                        5-CaO based glass composite coating. Samples were characterized by X-ray diffraction (XRD) analysis and it was shown that they were constituted of an HA matrix with small traces of tricalcium phosphate (β-TCP) phase. The XRD spectra were quite similar to those of commercial HA coatings. For coating to substrate adhesion two different tests were performed: adhesion tests according to ASTM C 633-79 and ASTM D 1002-83 standards. Higher values were obtained for ASTM C 633-79 than for ASTM D 1002-83, 35 and 15 MPa, respectively. This behavior is discussed in terms of the different stress effort applied to specimens. The results obtained with ASTM C 633-79 show that these double layered satisfy the stress requirements for load bearing biomedical applications. {\textcopyright} 1998 Published by Elsevier Science S.A.
},
	keywords = {Adhesion, Ceramic coatings, Characterization, coating, Hydroxyapatite glass ceramic coatings, materials testing, Mechanical properties, Microstructure, Phosphates, Plasma spraying, Stresses, Titanium aluminum vanadium substrates, X ray diffraction analysis, X-Ray Diffraction},
	isbn = {02578972 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032047796\&partnerID=40\&md5=17fc09096386c9573405b63de4522e69},
	author = {Silva, P.L. and Santos, J.D. and Monteiro, F.J. and Knowles, J.C.}
}
@article {2584,
	title = {Chromium determination in osteoblast-like cell culture medium by catalytic cathodic stripping voltammetry with a mercury microelectrode},
	journal = {Journal of Trace Elements in Medicine and BiologyJ. Trace Elem. Med. Biol.},
	volume = {12},
	year = {1998},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JTEBFPubMed ID: 9760419Language of Original Document: EnglishCorrespondence Address: Morais, S.; Inst. Engenharia Biomedica (INEB), Praca Coronel Pacheo 1, 4050 Porto, PortugalChemicals/CAS: Acetates; Chromium, 7440-47-3; Culture Media; Mercury, 7439-97-6; NitratesReferences: Ferguson Jr., A.B., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J. Bone Joint Surg., 42 A, pp. 77-90;Ferguson Jr., A.B., Akahoshi, Y., Laing, P.G., Hodge, E.S., Trace metal ion concentration in the liver, kidney, spleen and lung of normal rabbits (1962) J Bone Joint Surg., 44 A, pp. 317-322; 
Ferguson Jr., A.B., Akahoshi, Y., Laing, P.G., Hodge, E.S., Characteristics of trace ions released from embedded metal implants in the rabbit (1962) J. Bone Joint Surg. 1962, 44 A, pp. 323-336; 
Lux, F., Ziesler, R., Investigations of the corrosive deposition of components of metal implants and of the behaviour of biological trace elements in metallosis tissue by means of instrumental multi-elemental activation analysis (1974) J. Radioanal. Chem., 19, pp. 289-297; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 223-247. , ASTM STP 859 (Fraker, A. C. and Griffin, C. D. ,Eds.). American Society for Testing and Materials, Philadelphia; 
Minski, M.J., Dobbs, H.S., (1980) Trace Element Analytical Chemistry in Medicine and Biology, pp. 339-350. , Bratter, P., Schramel, P., Eds., Walter de Gruyter, Berlin; 
Smith, G.K., (1982) Systemic Transport and Distribution of Iron and Chromium from 316L Stainless Steel Implants, , Ph.D. Thesis, University of Pennsylvania, Philadelphia; 
Brown, S.A., Farnsworth, L.J., Merritt, K., Crowe, T.D., In vitro and in vivo metal ion release (1988) J. Biomed. Materials Research, 22, pp. 321-338; 
Lucas, L.C., Bearden, L.J., Lemons, J.E., Ultrastructural examination of in vitro and in vivo cells exposed to elements from type 316L stainless steel (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 208-222. , ASTM STP 859 (Fraker, A. C. and Griffin, C. D., Eds.), American Society for Testing and Materials, Philadelphia; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , ASTM STP859 (Fraker, A. C. and Griffin, C. D., Eds.), American Society for Testing and Materials, Philadelphia; 
Smith, G.K., Black, J., Models for systemic effects of metallic implants (1977) NBS Special Publication 472, pp. 23-28. , Weinstein, A., Eds., U. S. Government Printing Office, Washington D. C; 
Deutman, R., Mulder, T.J., Brian, R., Nater, J.P., Metal sensitivity before and after total hip arthroplasty (1977) J. Bone Jt. Surg., 59 A, pp. 862-865; 
Crosmun, S.T., Mueller, T.R., The determination of chromium (VI) in natural waters by differential pulse polarography (1975) Anal. Chim. Acta, 75, pp. 199-205; 
Golimowski, J., Valenta, P., Nurnberg, H.W., Trace determination of chromium in various water types by adsorption differential pulse polarography (1985) Fresenius Z. Anal. Chem., 322, pp. 315-322; 
Boussemart, M., Van Den Berg, C.M.G., Ghaddaf, M., The determination of the chromium speciation in sea water using catalytic cathodic stripping voltammetry (1992) Anal. Chim. Acta, 262, pp. 103-115; 
Paneli, M., Voulgaropoulos, A.V., Kalcher, K., The catalytic adsorptive stripping voltammetry determination of chromium with TTHA and nitrate (1993) Mikrochim. Acta, 110, pp. 205-215; 
Wang, J., Lu, J.A., Olsen, K., Measurement of ultratrace levels of chromium by adsorptive-catalytic stripping voltammetry in the presence of cupferron (1992) Analyst, 117, pp. 1913-1917; 
Nurnberg, H.W., Studies on toxic trace metals in the environment by advanced polarographic methods (1978) Proc. Analyt. Div. Chem. Soc., 15, pp. 275-283; 
Ottaway, J.M., Fell, G.S., Determination of chromium in biological materials (1986) Pure and Applied Chemistry, 58, pp. 1707-1720; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9, pp. 791-795; 
Morais, S., Carvalho, G.S., Sousa, J.P., Determination of nickel in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9, pp. 422-426; 
Morais, S., Carvalho, G.S., Sousa, J.P., Potentiometric determination of total and ionised calcium in osteoblast-like cell culture medium (1996) Electroanalysis, 8, pp. 1174-1178; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralisation by osteoblast-like cells: Part I- Retardation of tissue mineralisation by metal salts (1998) Biomaterials, 19, pp. 13-21; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., Bruijn, J.D., Van Blitterswijk, C.A., Decreased consumption of Ca and P during in vitro biomineralisation and biologically-induced deposition of Ni and Cr in the presence of stainless steel corrosion products (1998) J. Biomed. Mater. Res., , in press; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions (1998) J. Biomed. Mater. Res., , in press; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res., 254, pp. 317-330; 
Kotz, L., Henze, G., Kaiser, G., Pahlke, S., Veber, M., Tolg, G., Wet mineralisation of organic matrices in glassy carbon vessels in a pressure-bomb system for trace element analysis (1979) Talanta, 26, pp. 681-691; 
Peng, J., Jin, W., Anodic stripping voltammetric determination of trace lead in micro-samples with a mercury-ultramicroelectrode (1992) Anal. Chim. Acta, 264, pp. 213-219; 
Wehmeyer, K.R., Wightman, R.M., Cyclic voltammetry and anodic stripping voltammetry with mercury ultramicroelectrodes (1985) Anal. Chem., 57, pp. 1989-1993; 
Wightman, R.M., Wiptd, O., Voltammetry at ultramicroelectrodes (1989) Electroanalytical Chemistry, 15, pp. 335-336. , Bard, A. J., Ed., Marcel, Dekker, New York; 
Wurfels, M., Jackwerth, E., Stoeppler, M., Residues from biological materials after pressure decomposition with nitric acid Part 3 a (1989) Anal. Chim. Acta, 226, pp. 31-41; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralisation by osteoblast-like cells: Part II- Characterisation of cellular culture supernatants (1998) Biomaterials, 19, pp. 23-29; 
Wurfels, M., Jackwerth, E., Stoeppler, M., Residues from biological materials after pressure decomposition with nitric acid Part 2 a (1989) Anal. Chim. Acta, 226, pp. 17-30; 
Gilbert, T.R., Clay, A.M., Determination of chromium in sea water by atomic absorption spectrometry (1973) Anal. Chim. Acta, 67, pp. 289-295; 
Ping, L., Matsumoto, K., Fuwa, K., Determination of urinary chromium levels for healthy men and diabetic patients by electric thermal atomic absorption spectrometry (1983) Anal. Chim. Acta, 147, pp. 205-212; 
Torrance, K., Gatford, C., Determination of soluble chromium in simulated PWR coolant by differential-pulse adsorptive stripping voltammetry (1987) Talanta, 34, pp. 939-944; 
Lanza, P., Taddia, M., The differential pulse polarographic determination of chromium in gallium arsenide (1984) Anal. Chim. Acta, 157, pp. 37-44; 
Miller, J.N., Basic statistical methods for analytical chemistry. Part 2: Calibration and regression (1991) Analyst, 116, pp. 3-14; 
Ringbom, A., (1963) Complexation in Analytical Chemistry, pp. 332-333. , Elving, P. J. and Kolthoff, I. M., Eds, Interscience Publishers, New York/London; 
Black, J., Maitin, E.C., Gelman, H., Morris, D.M., Serum concentrations of Cr, Co, and Ni after total hip replacement: A six months study (1983) Biomaterials, 4, pp. 160-164; 
Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8; 
Dobbs, H.S., Minski, J., Metal ion release after total hip replacement (1980) Biomaterials, 1, pp. 193-198; 
Katz, S., Salem, H., The toxicology of chromium with respect to its speciation: A review (1993) J. Applied Toxicology, 13, pp. 217-224; 
Bianchi, V., Toso, R.D., Debetto, P., Mechanisms of chromium toxicity in mammalian cell cultures (1980) Toxicology, 17, pp. 219-224; 
Hopkins, L.L., Schwartz, K., Chromium (III) binding to serum proteins, specifically siderophilin (1964) Biochem. Biophys. Acta, 90, pp. 484-491; 
Nurnberg, H.W., The voltammetric approach in trace metal chemistry of natural waters and atmospheric precipitation (1984) Anal. Chim. Acta, 164, pp. 1-21
},
	month = {1998///},
	pages = {101 - 108},
	abstract = {A catalytic cathodic stripping voltammetric procedure for the determination of total chromium in osteoblast-like cell culture medium using a mercury film microelectrode (MFM) was optimised. The method is based on the pre-concentration of the Cr(III)-diethylenetriaminepentaacetic acid (DTPA) complex by adsorption at the potential of -1.00 V (vs. Ag/AgCl) in the presence of 10 x 10},
	keywords = {Acetates, animal cell, Animalia, Animals, article, atomic absorption spectrometry, Bone Marrow Cells, catalysis, Cell culture, Cells, Cultured, Chromium, complex formation, controlled study, Culture Media, culture medium, drug determination, electrochemistry, Electrodes, Hydrogen-Ion Concentration, in vitro study, Kinetics, Mercury, microelectrode, Microelectrodes, Nitrates, nonhuman, osteoblast, Osteoblasts, potentiometry, priority journal, Rabbits, Reproducibility of Results, sensitivity and specificity, Stromal Cells, technique},
	isbn = {0946672X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-7344255754\&partnerID=40\&md5=16bbb746d7dcdd04b95a01edcc764771},
	author = {Morais, S. and De Carvalho, G.S. and Sousa, J.P.}
}
@article {2579,
	title = {Decreased consumption of Ca and P during in vitro biomineralization and biologically induced deposition of Ni and Cr in presence of stainless steel corrosion products},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {42},
	year = {1998},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(199811)42:2<199::AID-JBM5>3.0.CO;2-KPubMed ID: 9773816Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Universidade do Porto, Rua Manuel Pereira da Silva, 4200 Porto, PortugalChemicals/CAS: Biocompatible Materials; Calcium, 7440-70-2; Chromium, 7440-47-3; Nickel, 7440-02-0; Phosphorus, 7723-14-0; Steel, 12597-69-2References: Ferguson Jr., B., Akahoshi, Y., Laing, P.G., Hodge, E.S., Trace metal ion concentration in the liver, kidney, spleen and lung of normal rabbits (1962) J. Bone Joint Surg., 44 A, pp. 317-322;Ferguson Jr., A.B., Akahoshi, Y., Laing, P.G., Hodge, E.S., Characteristics of trace ions released from embedded metal implants in the rabbit (1962) J. Bone Joint Surg., 44 A, pp. 323-336; 
Ferguson Jr., A.B., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J. Bone Joint Surg., 42 A, pp. 77-90; 
Lux, F., Ziesler, R., Investigations of the corrosive deposition of components of metal implants and of the behavior of biological trace elements in metallosis tissue by means of instrumental multi-elemental activation analysis (1974) J. Radioanal. Chem., 19, pp. 289-297; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, ASTM STP 859, pp. 223-247. , A. C. Fraker and C. D. Griffin (eds.), American Society for Testing and Materials, Philadelphia, Pennsylvania; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, ASTM STP 859, pp. 195-207. , A. C. Fraker and C. D. Griffin, (eds.), American Society for Testing and Materials, Philadelphia, Pennsylvania; 
Smith, G.K., Black, J., Models for systemic effects of corrosion products (1977) Retrieval and Analysis of Orthopedics Implants, pp. 23-28. , A. Weinstein D. Gibbons, S. A. Brown, and A. W. Ruff (eds.), NBS Special Publication 472, U.S. Government Printing Office, Washington, D.C; 
Deutman, R., Mulder, T.J., Brian, R., Nater, J.P., Metal sensitivity before and after total hip arthroplasty (1977) J. Bone Joint Surg., 59 A, pp. 862-865; 
Verne, J., Sannie, C., Etude de l{\textquoteright}action toxique des cations sur les fibroblastes cultiv{\'e}s in vitro (1933) Compt. Rend. Acad. Sci., 196, pp. 1246-1247; 
Kirkpatrick, C.J., A critical view of current and proposed methodologies for biocompatibility testing: Cytotoxicity in vitro (1992) Regul. Affairs, 4, pp. 13-22; 
Pereira, M.L., Silva, A., Tracana, R., Carvalho, G.S., Toxic effects caused by stainless steel corrosion products on mouse seminiferous cells (1994) Cytobios, 77, pp. 73-80; 
Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Chromium accumulation and ultrastructural changes in the mouse liver caused by stainless steel corrosion products (1995) J. Mater. Sci. Mater. Med., 6, pp. 523-527; 
Tracana, R.B., Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Stainless steel corrosion products cause alterations on mouse spleen cellular populations (1995) J. Mater. Sci. Mater. Med., 6, pp. 56-61; 
Bravo, I., Sousa, S.R., Carvalho, G.S., Barbosa, M., Metals electrochemically dissolved in human serum inhibit T-lymphocyte function (1992) Advances in Biomaterials, pp. 7-11. , P. J. Doherty et al. (eds.), Amsterdam, Elsevier; 
Carvalho, G.S., Castanheira, M., Diogo, I., Abreu, A.M., Sousa, J.P., Loon, J.A., Blitterswijk, C.A., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1995) J. Mater. Sci. Mater. Med., 7, pp. 77-83; 
Evans, E.M., Freeman, M.A.R., Miller, A.J., Vernon-Roberts, B., Metal sensitivity as a cause of bone necrosis and loosening of the prosthesis in total joint replacement (1974) J. Bone Joint Surg., 56 B, pp. 626-642; 
Betts, F., Wright, T., Salvati, E.A., Boskey, A., Mansal, M., Cobalt alloy metal debris in periarticular tissues from total hip revision arthoplasties (1992) Clin. Orthop., 278, pp. 75-82; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralization by osteoblast-like cells: Part 1-Retardation of tissue mineralization by metal salts (1998) Biomaterials, 19, pp. 13-21; 
Morais, S., Carvalho, G.S., Sousa, J.P., Potentiometric determination of total and ionized calcium in osteoblast-like cell culture medium (1996) Electroanalysis, 8, pp. 1174-1178; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9, pp. 791-795; 
Morais, S., Carvalho, G.S., Sousa, J.P., Electrochemical characterization of iron species in osteoblast-like cell culture medium (1995) Port. Electrochim. Acta, 13, pp. 483-487; 
Morais, S., Carvalho, G.S., Sousa, J.P., Determination of nickel in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with mercury microelectrodes (1997) Electroanalysis, 9, pp. 422-426; 
Morais, S., Carvalho, G.S., Sousa, J.P., Optimization study for nickel quantification in cell culture medium using mercury film microelectrodes (1996) Port. Electrochim. Acta, 14, pp. 267-272; 
Morais, S., Carvalho, G.S., Sousa, J.P., Chromium determination in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode J. Trace Elements Med. Biol., , in press; 
Nurnberg, H.W., The voltammetric approach in trace metal chemistry of natural waters and atmospheric precipitation (1984) Anal.Chim. Acta, 164, pp. 1-21; 
Merritt, K., Wortman, R.S., Millard, M., Brown, S.A., XPS analysis of 316 LVM corroded in serum and in saline (1983) Biomater. Med. Devices Artif. Org., 11, pp. 115-127; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res., 254, pp. 317-330; 
Fiske, H., Subbarow, Y., The colorimetric determination of phosphorus (1925) J. Biol. Chem., 66, pp. 373-400; 
Vrouwenvelder, W.C.A., Groot, C.G., De Groot, K., Histological and biochemical evaluation of osteoblasts cultured on bioactive glass, hydroxylapatite, titanium alloy, and stainless steel (1993) J. Biomed. Mater. Res., 27, pp. 465-475; 
Bessey, O.A., Lowry, O.H., Breck, M.J., A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum (1946) J. Biol. Chem., 164, pp. 321-326; 
Karster, U., Wollenberger, A., Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates (1977) Anal. Biochem., 77, pp. 464-470; 
McGee-Russell, S.M., Histochemical methods for calcium (1958) J. Histochem. Cytochem., 6, pp. 22-42; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulte formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., Tassinari, M.S., Stein, G.S., Lian, J.B., Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells (1990) J. Cell Physiol., 143, pp. 213-221; 
Gotoh, Y., Hiraiwa, K., Narajama, M., In vitro mineralization of osteoblastic cells derived from human bone (1990) Bone Miner., 8, pp. 239-250; 
Cheng, S.L., Yang, J.W., Rifas, L., Zhang, S.F., Avioli, L.V., Differentiation of human bone marrow osteogenic stromal cells in vitro. Induction of the osteoblast phenotype by dexamethasone (1994) Endocrinology, 134, pp. 277-286; 
Gundle, R., Joyner, C.J., Triffit, J.T., Human bone tissue formation in diffusion chamber culture in vivo by bone derived cells and marrow stromal fibroblastic cells (1995) Bone, 16, pp. 597-601; 
Berridge, V., Tan, A.S., Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction (1993) Arch. Biochem. Biophys, 303, pp. 474-482; 
Ciapetti, G., Cenni, E., Stea, S., Pizzoferrato, A., In vitro evaluation of cell/biomaterial interaction by MTT assay (1993) Biomaterials, 14, pp. 359-364; 
Sgouras, D., Duncan, R., Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1-Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity (1990) J. Mater. Sci. Mater. Med., 1, pp. 61-68; 
Martin, A., Clynes, M., Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays (1993) Cytotechnology, 11, pp. 49-58; 
Stein, G.S., Lion, J.B., Molecular mechanisms mediated proliferation-differentiation interrelationships during progressive development of the osteoblast phenotype: Update 1995 (1995) Endosc. Rev., 4, pp. 290-297; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., The ultradtructure of the bone hydroxyapatite interface in vitro (1992) J. Biomed, Mater. Res., 26, pp. 1365-1382; 
Davies, J.E., Chernecky, R., Lowenberg, B., Shiga, A., Deposition and resorption of calcified matrix in vitro by rat marrow cells (1991) Cells Mater., 1, pp. 3-15; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., De Bruijn, J.D., Van Blitterswijk, C.A., Effects of AISI 316L corrosion products in in vitro bone formation (1998) Biomaterials, 19, pp. 999-1007; 
Pohler, E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, pp. 158-228. , L. R. Rubin (ed.), C. V. Mosby, St. Louis, Missouri; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Initiation and progression of mineralization of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphate (1991) Bone Miner., 14, pp. 27-40; 
Bellows, C.G., Heersche, J.N.M., Aubin, J.E., Inorganic phosphate added exogenously or released from β-glycerophosphate initiates mineralization of osteoid nodules in vitro (1992) Bone Miner., 17, pp. 15-29; 
Chung, C.H., Golub, E.E., Forbes, E., Tokuoka, T., Shapiro, I.M., Mechanisms of action of β-glycerophosphate on bone cell mineralization (1992) Calcif. Tissue Int., 51, pp. 305-311; 
Boskey, A.L., Stiner, D., Doty, S.B., Binderman, I., Levoy, P., Studies of mineralization in tissue culture: Optimal conditions for cartilage calcification (1992) Bone Miner., 16, pp. 11-36; 
Rae, T., The toxicity of metals used in orthopaedic prostheses (1981) J. Bone Joint Surg., 63 B, pp. 435-440; 
Webb, M., Weinzierl, S.M., Uptake of 63Ni 2+ from its complexes with proteins and other ligands by mouse dermal fibroblasts in vitro (1972) Br. J. Cancer, 26, pp. 292-298; 
Katz, S.A., Salem, H., The toxicology of chromium with respect to its speciation: A review (1993) J. Appl.Toxicol., 13, pp. 217-224; 
Pules, D.A., Huh, W.W., Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells (1995) J. Appl. Biomater., 6, pp. 109-116; 
Thompson, G.J., Pules, D.A., Effects of sublethal metal ion concentrations on osteogenic cells derived from bone marrow stromal cells (1995) J. Appl. Biomater., 6, pp. 249-258
},
	month = {1998///},
	pages = {199 - 212},
	publisher = {John Wiley \& Sons Inc},
	abstract = {The purpose of this study was to investigate the effects of 316L stainless steel (SS) corrosion products on the in vitro biomineralization process, because tissue necrosis, bone loss, impaired bone mineralization, and loosening of orthopedic implants are associated with ions and debris resulting from biodegradation. Rat bone marrow cells were cultured in experimental conditions that favored the proliferation and differentiation of osteoblastic cells and were exposed to SS corrosion products obtained by electrochemical means for periods ranging from 1 to 21 days. Quantification of total and ionized Ca and P, as well as Fe, Cr, and Ni, ions in the culture media of control and metal added cultures during the incubation period was performed to study the influence of corrosion products on the Ca and P consumption that occurs during the mineralization process. Control cultures and metal effects on cultures were evaluated concerning DNA content, enzymatic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and alkaline phosphatase (ALP) activity. Histochemical detection of ALP, Ca, and phosphate deposition, and examination of the cultures by scanning and transmission electron microscopy (SEM and TEM) were also performed. The presence of SS corrosion products resulted in impairment of the normal behavior of rat bone marrow cultures. Levels of Cr and Ni in the medium of cultures exposed to 316L SS corrosion products decreased throughout the incubation period, suggesting a regular deposition of these species; these results were supported by TEM observation of the cultures. Cultures exposed to the corrosion products presented lower DNA content, MTT reduction, and ALP activity and failed to form mineralized areas. These cultures showed negative staining on histochemical reactions for the identification of calcium and phosphate deposition and SEM and TEM examination did not show mineral globular structures or mineralization foci, respectively, which is characteristic of cultures grown in control conditions. These results suggest that metal ions associated with 316L SS are toxic to osteogenic cells, affecting their proliferation and differentiation.The purpose of this study was to investigate the effects of 316L stainless steel (SS) corrosion products on the in vitro biomineralization process, because tissue necrosis, bone loss, impaired bone mineralization, and loosening of orthopedic implants are associated with ions and debris resulting from biodegradation. Rat bone marrow cells were cultured in experimental conditions that favored the proliferation and differentiation of osteoblastic cells and were exposed to SS corrosion products obtained by electrochemical means for periods ranging from 1 to 21 days. Quantification of total and ionized Ca and P, as well as Fe, Cr, and Ni, ions in the culture media of control and metal added cultures during the incubation period was performed to study the influence of corrosion products on the Ca and P consumption that occurs during the mineralization process. Control cultures and metal effects on cultures were evaluated concerning DNA content, enzymatic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and alkaline phosphatase (ALP) activity. Histochemical detection of ALP, Ca, and phosphate deposition, and examination of the cultures by scanning and transmission electron microscopy (SEM and TEM) were also performed. The presence of SS corrosion products resulted in impairment of the normal behavior of rat bone marrow cultures. Levels of Cr and Ni in the medium of cultures exposed to 316L SS corrosion products decreased throughout the incubation period, suggesting a regular deposition of these species; these results were supported by TEM observation of the cultures. Cultures exposed to the corrosion products presented lower DNA content, MTT reduction, and ALP activity and failed to form mineralized areas. These cultures showed negative staining on histochemical reactions for the identification of calcium and phosphate deposition and SEM and TEM examination did not show mineral globular structures or mineralization foci, respectively, which is characteristic of cultures grown in control conditions. These results suggest that metal ions associated with 316L SS are toxic to osteogenic cells, affecting their proliferation and differentiation.
},
	keywords = {3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, alkaline phosphatase, animal cell, Animal cell culture, Animals, article, Biocompatible Materials, Biodegradation, biomineralization, Bioprosthesis, Bone, Bone and Bones, bone atrophy, bone marrow, Calcification, Physiologic, Calcium, calcium ion, Catalyst activity, Cells, Cultured, Chromium, controlled study, Corrosion, Corrosion products, Enzymes, Implants (surgical), metal implantation, mineralization, Nickel, nonhuman, osteoblast, Osteoblast-like cells, phosphate, phosphorus, prosthesis loosening, rat, Rats, Scanning electron microscopy, Stainless steel, Stainless steel corrosion products, Steel, Steel corrosion, tissue necrosis, transmission electron microscopy},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032210768\&partnerID=40\&md5=5275a87eebeeda6fdce8b3382dc3eaa5},
	author = {Morais, S. and Sousa, J.P. and Fernandes, M.H. and Carvalho, G.S. and De Bruijn, J.D. and Van Blitterswijk, C.A.}
}
@article {2585,
	title = {Effects of AISI 316L corrosion products in in vitro bone formation},
	journal = {BiomaterialsBiomaterials},
	volume = {19},
	year = {1998},
	note = {Cited By (since 1996): 38Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(97)00234-2PubMed ID: 9692798Language of Original Document: EnglishCorrespondence Address: Morais, S.; Universidade do Porto, Porto, PortugalChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; Calcium Phosphates; Calcium, 7440-70-2; Phosphorus, 7723-14-0; Stainless Steel, 12597-68-1References: Spector, M., Cease, C., Tong-Li, X., The local tissue response to biomaterials (1989) Crit Rev Biocompat, 5, pp. 269-295;Van Blitterswijk, C.A., Bakker, D., Hesseling, S.C., Koerten, H.K., Reactions of cells at implant surfaces (1991) Biomaterials, 12, pp. 187-193; 
Bagambisa, F.B., Kappert, H.F., Schilli, W., Interfacial reactions of osteoblasts to dental and implant materials (1994) J Oral Maxillofac Surg, 52, pp. 52-56; 
Bruijn, J.D., Van Blitterswijk, C.A., Davies, J.E., Initial bone matrix formation at the hydroxyapatite interface in vivo (1995) J Biomed Mat Res, 29, pp. 89-99; 
Pilliar, R.M., Porous-surface metallic implants for orthopedic applications (1987) J Biomed Mat Res, 21 (A1), pp. 1-33; 
Bobyn, J.D., Tanzer, M., Drygier, J.J., Du Joune, A.R., Brooks, C.E., Concerns with modularity in total hip arthroplasty (1994) Clin Orthop, 298, pp. 27-36; 
Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8; 
Dorr, L.D., Blocbaum, R., Emmanual, J., Meldrum, R., Histologic, biochemical and ion analysis of tissue and fluids retrieved during total hip arthroplasty (1990) Clin Orthop, 261, pp. 82-95; 
Hennig, F.F., Raithel, H.J., Schaller, K.H., Dohler, J.R., Nickel-chromand cobalt-concentrations in human tissue and body fluids of hip prosthesis patients (1992) J Trace Elem Electrolytes Health Dis, 6, pp. 239-243; 
Evans, E.M., Freeman, M.A.R., Miller, A.J., Vernon-Roberts, B., Metal sensitivity as a cause of bone necrosis and loosening of the prosthesis in total joint replacement (1974) J Bone Jt Surg, 56 B, pp. 626-642; 
Deutman, R., Rulder, T.J., Brian, R., Nater, J.P., Metal sensitivity before and after total hip arthroplasty (1977) J Bone Jt Surg, 59 A, pp. 862-865; 
Huo, M.H., Salvati, E.A., Lieberman, Jr., Betts, F., Bansal, M., Metallic debris in femoral endoseolysis in failed cemented total hip arthroplasties (1992) Clin Orthop, 276, pp. 157-168; 
Kirkpatrick, C.J., Mittermayer, C., Theoretical and practical aspects of testing potential biomaterials in vitro (1990) J Mat Mat Med, 1, pp. 9-13; 
Kirkpatrick, C.J., A critical view of current and proposed methodologies for biocompatibility testing: Cytotoxicity in vitro (1992) Regulatory Affairs, 4, pp. 13-22; 
Pizzoferrato, A., Ciapetti, G., Stea, S., Cell culture methods for testing biocompatibility (1994) Clin Mat, 15, pp. 173-190; 
Bizios, R., Mini review: Osteoblasts: An in vitro model of bone-implant interactions (1994) Biotechnol and Bioeng, 43, pp. 582-585; 
Pules, D.A., Huh, W.W., Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 109-116; 
Thompson, G.J., Pules, D.A., Effects of sublethal metal ion concentrations on osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 249-258; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., Effects of Co-Cr corrosion products and corresponding separate metal ions on human osteoblast-like cell cultures (1996) J Mater Sc Mater Med, 7, pp. 291-296; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., The use of rat, rabbit or human bone marrow derived cells for cytocompatibility evaluation of metallic elements (1997) J Mater Sc Mater Med, 8, pp. 233-238; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralisation by osteoblast-like cells: Part 1 - Retardation of tissue mineralisation by metal salts Biomaterials 1998; 
Pereira, M.L., Silva, A., Tracana, R., Carvalho, G.S., Toxic effects caused by stainless steel corrosion products on mouse seminiferous cells (1994) Cytobios, 77, pp. 73-80; 
Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Chromium accumulation and ultrastructural changes in the mouse liver caused by stainless steel corrosion products (1995) J Mater Sci Mater Med, 6, pp. 523-527; 
Tracana, R.B., Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Stainless steel corrosion products cause alterations on mouse spleen cellular populations (1995) J Mater Sci Mater Med, 6, pp. 56-61; 
Bravo, I., Sousa, S.R., Carvalho, G.S., Barbosa, M., Metals electrochemically dissolved in human serum inhibit T-lymphocyte function (1992) Advances in Biomaterials, pp. 7-11. , Doherty PJ et al., editors. Amsterdam: Elsevier; 
Carvalho, G.S., Castanheira, M., Diogo, I., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1995) J Mater Sci Mater Med, 7, pp. 77-83; 
Pohler, O.E.M., Degradation of metallic orthopedic implants Biomaterials in Reconstructive Surgery, pp. 158-228. , Rubin LR, editor. St. Louis: C. V. Mosby, 1983; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tiss Res, 254, pp. 317-330; 
McGee-Russell, S.M., Histochemical methods for calcium (1958) J Histochem Cytochem, 6, pp. 22-42; 
Morais, S., Carvalho, G.S., Sousa, J.P., Potentiometric determination of total and ionized calcium in osteoblast-like cell culture medium (1996) Electroanalysis, 8, pp. 1174-1178; 
Fiske, C.H., Subbarow, Y., The colorimetric determination of phosphorus (1925) J Biol Chem, 66, pp. 373-400; 
Miller, J.C., Miller, J.N., (1993) Statistics for Analytical Chemistry (3rd Ed.), pp. 32-35. , Massen M, Tyson J, Stockwell P, Miller J, Chalmers R, Harwood E, editors. New York, London: Ellis Horwood, PTR Prentice Hall; 
De Bruijn, J.D., Cpat, K., De Groot, K., Van Blitterswijk, C.A., The ultrastructure of the one-hydroxyapatite interface in vitro (1992) J Biomed Mat Res, 26, pp. 1365-1382; 
Davies, J.E., Chernecky, R., Lowenberg, B., Shiga, A., Deposition and resorption of calcified matrix in vitro by rat marrow cells (1991) Cells and Mat, 1, pp. 3-15; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulte formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells (1990) J Cell Phys, 143, pp. 213-221; 
Gotoh, Y., Hiraiwa, K., Narajama, M., In vitro mineralization of osteoblastic cells derived from human bone (1990) Bone Min, 8, pp. 239-250; 
Cheng, S.L., Yang, J.W., Riffins, L., Differentiation of human bone marrow osteogenic stromal cells in vitro. Induction of the osteoblast phenotype by dexamethasone (1994) Endocrinology, 134, pp. 277-286; 
Gundle, R., Joyner, C.J., Triffit, J.T., Human bone tissue formation in diffusion chamber culture in vivo by bone derived cells and marrow stromal fibroblastic cells (1995) Bone, 16, pp. 597-601; 
Bellows, C.G., Heersche, J.N.M., Aubin, J.E., Inorganic phosphate added exogenously or released from β-glycerophosphate initiates mineralization of osteoid nodules in vitro (1992) Bone Min, 17, pp. 15-29; 
Boskey, A.L., Stiner, D., Doty, S.B., Binderman, I., Levoy, P., Studies of mineralization in tissue culture: Optimal conditions for cartilage calcification (1992) Bone Min, 16, pp. 11-36; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Initiation and progression of mineralization of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphate (1991) Bone Min, 14, pp. 27-40; 
Chung, C.H., Golub, E.E., Forbes, E., Mechanisms of action of β-glycerophosphate on bone cell mineralization (1992) Calcif Tissue Int, 51, pp. 305-311
},
	month = {1998///},
	pages = {999 - 1007},
	publisher = {Elsevier Sci Ltd},
	abstract = {Rat bone marrow cells were cultured in experimental conditions that favour the proliferation and differentiation of osteoblastic cells (i.e., 2.52 x 10},
	keywords = {Absorption spectroscopy, alkaline phosphatase, animal cell, Animal cell culture, Animals, article, ascorbic acid, atomic absorption spectrometry, Bioassay, Biomaterials, bone development, bone marrow cell, Bone Marrow Cells, Calcium, Calcium phosphates, Cell culture, Cell Death, Cells, Cultured, Corrosion, Corrosion products, culture medium, dexamethasone, electrochemical analysis, electrochemistry, Enzymes, glycerophosphate, histochemistry, Histocytochemistry, in vitro study, male, metal ion, Mineralisation, mineralization, nonhuman, ossification, osteoblast, Osteoblast-like cells, Osteoblasts, phosphate, Phosphate minerals, phosphorus, priority journal, rat, Rats, Rats, Wistar, Scanning electron microscopy, Slurries, Stainless steel, Stainless steel 316L, Stainless-steel corrosion products, Steel corrosion},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032102774\&partnerID=40\&md5=1b60be114642b919f64414bb11673a19},
	author = {Morais, S. and Sousa, J.P. and Fernandes, M.H. and Carvalho, G.S. and De Bruijn, J.D. and Van Blitterswijk, C.A.}
}
@article {2580,
	title = {Flow cytometry for assessing biocompatibility},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {41},
	year = {1998},
	note = {Cited By (since 1996): 33Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19980915)41:4<649::AID-JBM17>3.0.CO;2-9PubMed ID: 9697038Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University of London, 256 Gray{\textquoteright}s Inn Rd., London WC1X 8LD, United KingdomChemicals/CAS: Biocompatible Materials; Collagen, 9007-34-5; Dental Implants; Fibronectins; Osteocalcin, 104982-03-8References: Kirkpatrick, C.J., A critical view of current and proposed methodologies for biocompatibility testing: Cytotoxicity in vitro (1992) Regul. Affairs, 4, pp. 13-32;Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Ariola, C.R., Granchi, D., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin. Mater., 15, pp. 173-190; 
Puleo, D.A., Preston, K.E., Shaffer, J.B., Bizios, R., Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques (1993) Biomaterials, 14, pp. 111-114; 
Clifford, C.J., Downes, S., A comparative study of the use of colorimetric assays in the assessment of biocompatibility (1996) J. Mater. Sci. Mater. Med., 7, pp. 637-643; 
Macey, M.G., (1994) Flow Cytometry: Clinical Applications, , Blackwell Scientific Publications, Oxford; 
Salzman, G.C., Singham, S.B., Johnston, R.G., Bohren, C.F., Light scattering and cytometry (1990) Flow Cytometry and Sorting, pp. 81-107. , M. R. Melamed, T. Lindmo, and M. L. Mendelsohn (eds.), Wiley-Liss, New York; 
Corver, W.E., Cornelisse, C.J., Fleuren, G.J., Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin and propidium iodide on a standard FACScan (1994) Cytometry, 15, pp. 117-128; 
Macon, W.R., Salhany, K.E., TI- T-cell subset analysis of paraffin-embedded peripheral T-cell lymphomas (PTCLs) with correlation of CD4/CD8 results by flow cytometry (FACs) (1997) Lab. Invest., 76, p. 753; 
Kuru, L., Parkar, M.H., Griffiths, G.S., Newman, H.N., Olsen, I., Flow cytometry analysis of functionally distinct gingival and periodontal ligament cells (1998) J. Dent. Res., 77, pp. 555-564; 
Kamiya, I., Okuda, K., Hara, K., Flow cytometric identification and detection of Porphromonas gingivalis by an LPS specific monoclonal antibody (1994) J. Periodontol., 65, pp. 309-315; 
Sumner, H., Abraham, D., Bou-Gharios, G., Plater-Zyberk, C., Olsen, I., Simultaneous measurement of cell surface and intracellular antigens by multiple flow cytometry (1991) J. Immunol. Methods, 136, pp. 259-267; 
Beauchamp, J.R., Abraham, D.J., Bou-Gharios, G., Partridge, T.A., Olsen, I., Expression and function of adhesion molecules during differentiation of human skeletal muscle in culture (1992) Am. J. Pathol., 140, pp. 387-401; 
Abraham, D., Lupoli, S., McWhirter, A., Plater-Zyberk, C., Piela, T.H., Korn, J., Olsen, I., Black, C., Expression and function of surface antigens on scleroderma fibroblasts (1991) Arthritis Rheum., 34, pp. 1164-1172; 
Bou-Gharios, G., Osman, J., Black, C., Olsen, I., Excess matrix accumulation in scleroderma is caused partly by differential regulation of stromelysin and TIMP-1 synthesis (1994) Clin. Chim. Acta, 231, pp. 69-78; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterisation of glass-reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Best, S., Sim, B., Kayser, M., Downes, S., The dependence of osteoblastic response on variations in the chemical composition and physical properties of hydroxyapatite (1997) J. Mater. Sci. Mater. Med., 8, pp. 97-103; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation and protein synthesis of human osteoblast-like cells (MG-63) (1995) J. Biomed. Mater. Res., 29, pp. 389-401; 
Granchi, D., Stea, S., Ciapetti, G., Savarino, L., Cavedagna, D., Pizzoferatto, A., In vitro effects of bone cements on the cell cycle of osteoblast-like cells (1995) Biomaterials, 16, pp. 1187-1192; 
Clover, J., Gowen, M., Are MG63 and HOS TE 85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? (1994) Bone, 15, pp. 585-591; 
Scharla, S.H., Strong, D.D., Rosen, C., Mohan, S., Holick, M., Baylink, D.J., Linkhart, T.A., 1,25-Dihydroxyvitamin D3 increases secretion of insulin-like growth factor binding protein-4 (IGFBP-4) by human osteoblast-like cells in vitro and elevates IGFBP-4 serum levels in vivo (1993) J. Clin. Endocrinol. Metabol., 77, pp. 1190-1197; 
De Bart, A.C., Quax, P.H., Lowik, C.W., Verheijen, J.H., Regulation of plasminogen activation, matrix metalloproteinases and urokinase-type plasminogen activator-mediated extracellular matrix degradation in human osteosarcoma cell line MG63 by interleukin-1 alpha (1995) J. Bone Min. Res., 19, pp. 1374-1384; 
Barille, S., Collette, M., Bataille, R., Amiot, M., Myeloma cells upregulate interleukin-6 secretion in osteoblastic cells through cell-to-cell contact but downregulate osteocalcin (1995) Blood, 86, pp. 3151-3159; 
Maillard, C., Malaval, L., Delmas, P.D., Immunological screening of SPARC/osteonectin in nonmineralized tissue (1992) Bone, 13, pp. 257-264; 
Bou-Gharios, G., Osman, J., Atherton, A., Monoghan, P., Vancheeswaran, R., Black, C., Olsen, I., Expression of ectopeptidases in scleroderma (1995) Ann. Rheum. Dis., 54, pp. 111-116; 
Goseki, T., Shimizu, N., Iwasawa, T., Takiguchi, H., Abiko, Y., Effects of in vitro cellular aging on alkaline phosphatase, cathepsin activities and collagen secretion of human peridontal ligament-derived cells (1996) Mech. Age. Dev., 91, pp. 1761-11183; 
Hallab, N.J., Bundy, K.J., O{\textquoteright}Connor, K., Clark, R., Moses, R.L., Cell adhesion to biomaterials: Correlations between surface charge, surface roughness, adsorbed protein and cell morphology (1995) J. Long-Term Effects Med. Implants, 5, pp. 209-231; 
Kieswetter, K., Schwartz, Z., Hummert, T.W., Cochran, D.L., Simpson, J., Sean, D.P., Boyan, B.D., Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells (1996) J. Biomed. Mater. Res., 32, pp. 55-63; 
Altankov, G., Grinnell, F., Groth, T., Studies on the biocompatibility of materials: Fibroblast reorganization of substratumbound fibronectin on surfaces varying in wettability (1996) J, Biomed. Mater. Res., 30, pp. 385-391; 
Chesmel, K.D., Clark, C.C., Brighton, C.T., Black, J., Cellular response to chemical and morphologic aspects of biomaterial surfaces. II. The biosynthetic and migratory response of bone cell populations (1995) J. Biomed. Mater. Res., 29, pp. 1101-1110; 
Nishizawa, K., Toriyama, M., Suzuki, T., Kawamoto, Y., Yokogawa, Y., Nagae, H., Effects of the surface wettability and zeta potential of bioceramics on the adhesiveness of anchorage-dependent animal cells (1993) J. Ferment. Bioeng., 75, pp. 435-437; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J. Biomed. Mater. Res., 39, pp. 244-251
},
	month = {1998///},
	pages = {649 - 656},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Flow cytometry (FCM) was examined as a possible procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery. The human osteoblast-like cell line MG63 was grown on hydroxyapatite (HA) and P                        2O
                        5 glass-reinforced HA composite discs and compared with the same cells grown on polystyrene culture dishes. While morphological observation at the light and electron microscopic levels showed no major deleterious effects, FCM indicated that cell size was somewhat reduced, particularly by growth on the HA composite. Moreover, this material also appeared to delay the progression of the cells from the G0/G1 into the S phase of the cell cycle. In addition to this low level of inhibition of cell growth relative to control cultures, FCM analysis also demonstrated that the glass-reinforced HA caused some down-regulation of the expression of osteocalcin and fibronectin, two antigens which play a vital part in the integrity and function of bone and soft connective tissue, respectively. These results thus show, first, that although HA and the HA composite used in these experiments were generally biocompatible, they nevertheless had certain suboptimal effects on the cells; and second, that FCM could be a highly useful procedure for effectively screening and evaluating important biological responses to implant materials.
Flow cytometry (FCM) was examined as a possible procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery. The human osteoblast-like cell line MG63 was grown on hydroxyapatite (HA) and P
                        2O
                        5 glass-reinforced HA composite discs and compared with the same cells grown on polystyrene culture dishes. While morphological observation at the light and electron microscopic levels showed no major deleterious effects, FCM indicated that cell size was somewhat reduced, particularly by growth on the HA composite. Moreover, this material also appeared to delay the progression of the cells from the G0/G1 into the S phase of the cell cycle. In addition to this low level of inhibition of cell growth relative to control cultures, FCM analysis also demonstrated that the glass-reinforced HA caused some down-regulation of the expression of osteocalcin and fibronectin, two antigens which play a vital part in the integrity and function of bone and soft connective tissue, respectively. These results thus show, first, that although HA and the HA composite used in these experiments were generally biocompatible, they nevertheless had certain suboptimal effects on the cells; and second, that FCM could be a highly useful procedure for effectively screening and evaluating important biological responses to implant materials.
},
	keywords = {antigen expression, Antigens, Biocompatibility, Biocompatible Materials, biomaterial, Biomaterials, Biomedical engineering, Bone and Bones, bone prosthesis, Cell culture, cell cycle, cell cycle s phase, Cell Line, Cell size, cell ultrastructure, Cells, Collagen, conference paper, Dental Implants, dentistry, extracellular matrix, Fibronectin, Fibronectins, flow cytometry, Glass fiber reinforced plastics, growth inhibition, human, human cell, Humans, hydroxyapatite, Implants, Implants (surgical), Microscopy, Electron, Scanning, mitosis inhibition, morphology, orthopedics, Osteoblasts, osteocalcin, Polystyrenes, Prostheses and Implants, prosthesis material, Tissue, tooth prosthesis},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032531299\&partnerID=40\&md5=5ea21d124ffce48018967f988cac815c},
	author = {Lopes, M.A. and Knowles, J.C. and Kuru, L. and Santos, J.D. and Monteiro, F.J. and Olsen, I.}
}
@article {2594,
	title = {In vitro biomineralization by osteoblast-like cells I. Retardation of tissue mineralization by metal salts},
	journal = {BiomaterialsBiomaterials},
	volume = {19},
	year = {1998},
	note = {Cited By (since 1996): 39Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(97)00149-XPubMed ID: 9678845Language of Original Document: EnglishCorrespondence Address: Carvalho, G.S.; IEC, Universidade do Minho, Largo do Paco, 4719 Braga Codex, PortugalChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; Calcium, 7440-70-2; Cations; Chromium, 7440-47-3; Iron, 7439-89-6; Metals; Nickel, 7440-02-0; Phosphates; Stainless Steel, 12597-68-1; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1References: Evans, E.M., Freeman, M.A.R., Miller, A.J., Vernon-Roberts, B., Metal sensitivity as a cause of bone necrosis and loosening of the prosthesis in total joint replacement (1974) J Bone Jt Surg, 56 B, pp. 626-642;Deutman, R., Rulder, T.J., Brian, R., Nater, J.P., Metal sensitivity before and after total hip arthroplasty (1977) J Bone Jt Surg, 59 A, pp. 862-865; 
Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8; 
Dorr, L.D., Blocbaum, R., Emmanual, J., Meldrum, R., Histologic, biochemical and ion analysis of tissue and fluids retrieved during total hip arthroplasty (1990) Clin Orthop, 261, pp. 82-95; 
Henning, F.F., Raithel, H.J., Schaller, K.H., Dohler, J.R., Nickel-, chromium- and cobalt-concentrations in human tissue and body fluids of hip prosthesis patients (1992) J Trace Elem Electrolytes Health Dis, 6, pp. 239-243; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., Effects of Co-Cr corrosion products and corresponding separate metal ions on human osteoblast-like cell cultures (1996) J Mater Sci Mater Med, 7, pp. 291-296; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., The use of rat, rabbit or human bone marrow derived cells for cytocompatibility evaluation of metallic elements (1997) J Mater Sci Mater Med, 8, pp. 233-238; 
Pules, D.A., Huh, W.W., Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 109-116; 
Thompson, G.J., Pules, D.A., Effects of sublethal metal ion concentrations on osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 249-258; 
McGee-Russell, S.M., Histochemical methods for calcium (1958) J Histochem Cytochem, 6, pp. 22-42; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Wong, M., Rao, L.G., Li, H., Long-term effects of physiologic concentrations of dexamethasone on human bone derived cells (1990) J Bone Miner Res, 5, pp. 803-813; 
Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells (1990) J Cell Phys, 143, pp. 213-221; 
Gotoh, Y., Hiraiwa, K., Narajama, M., In vitro mineralisation of osteoblastic cells derived from human bone (1990) Bone Miner, 8, pp. 239-250; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Initiation and progression of mineralisation of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphate (1991) Bone Miner, 14, pp. 27-40; 
Bellows, C.G., Heersche, J.N.M., Aubin, J.E., Inorganic phosphate added exogenously or released from β-glycerophosphate initiates mineralisation of osteoid nodules in vitro (1992) Bone Miner, 17, pp. 15-29; 
Chung, C.H., Golub, E.E., Forbes, E., Mechanisms of action of β-glycerophosphate on bone cell mineralisation (1992) Calcif Tissue Int, 51, pp. 305-311; 
Schimdt, R., Kulbe, K.D., Long term cultivation of human osteoblasts (1993) Bone Miner, 20, pp. 211-221; 
Cheng, S.L., Yang, J.W., Riffins, L., Differentiation of human bone marrow osteogenic stromal cells in vitro. Induction of the osteoblast phenotype by dexamethasone (1994) Endocrinology, 134, pp. 277-286; 
Gundle, R., Joyner, C.J., Triffit, J.T., Human bone tissue formation in diffusion chamber culture in vivo by bone derived cells and marrow stromal fibroblastic cells (1995) Bone, 16, pp. 597-601; 
Fernandes, M.H., Costa, M.A., Carvalho, G.S., Mineralisation in serially passaged human alveolar bone cells (1997) J Mater Sci Mater Med, 8, pp. 73-76; 
Martin, A., Clynes, M., Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays (1993) Cytotechnology, 11, pp. 49-58; 
Sgouras, D., Duncan, R., Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use. 1. Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity (1990) J Mater Sci Mater Med, 1, pp. 61-68; 
Ciapetti, G., Cenni, E., Stea, S., Pizzoferrato, A., In vitro evaluation of cell/biomatrial interaction by MTT assay (1993) Biomaterials, 14, pp. 359-364; 
Yoshimura, T., Kurita, C., Hayata, M., Nagai, H., Diagnosis of drug allergy by the lymphocyte stimulation test with the MTT (3-(4,5-dimethyl thiazol-2-yl)-2,2-diphenyl tetrazolium bromide) assay (1993) Biol Pharm Bull, 16, pp. 686-689; 
Sasanaluckit, P., Albustany, K.R., Doherty, P.J., Williams, D.F., Biocompatibility of glass ionomer cements (1993) Biomaterials, 14, pp. 906-916; 
Carvalho, G.S., Castanheira, M., Diogo, I., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1996) J Mater Sci Mater Med, 7, pp. 77-83; 
Vrouwenvelder, W.C.A., Groot, C.G., De Groot, K., Histological and biochemical evaluation of osteoblasts cultured on bioactive glass, hydroxylapatite, titanium alloy and stainless steel (1993) J Biomed Mater Res, 27, pp. 465-475; 
Owen, M., Marrow stromal stem cells (1988) J Cell Sci, 10, pp. 63-76; 
Beresford, J.N., Osteogenic stem cells and the stromal system of bone and marrow (1989) Clin Orthop Rel Res, 240, pp. 270-280; 
Owen, T.A., Arohow, M., Shalhonb, V., Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation during formation of bone extracellular matrix (1990) J Cell Phys, 143, pp. 420-430; 
Malaval, L., Modrowski, D., Gupta, K., Aubin, J.E., Cellular expression of bone related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures (1994) J Cell Phys, 158, pp. 555-572; 
Stein, G.S., Lion, J.B., Molecular mechanisms mediated proliferation-differentiation interrelationships during progressive development of the osteoblast phenotype: Update 1995 (1995) End Rev, 4, pp. 290-297
},
	month = {1998///},
	pages = {13 - 21},
	publisher = {Elsevier Sci Ltd},
	abstract = {The cytocompatibility of stainless steel 316L (SS 316L) corrosion products was investigated with particular focus on the dose- and time-effect of electrochemically dissolved SS and the corresponding separate metal ions on osteogenic bone marrow derived cells. Type AISI 316L stainless steel (Fe 63.9\%, Cr 18.0\%, Ni 12.5\%, Mo 2.8\%, Si 1.2\%, Mn 1.6\% and C 0.025\%, weight for weight) was anodically dissolved in Hank{\textquoteright}s Balanced Salt Solution (HBSS) and diluted to the following concentrations: 500 μgml},
	keywords = {Absorption spectroscopy, alkaline phosphatase, animal cell, Animalia, Animals, article, ascorbic acid, atomic absorption spectrometry, balanced salt solution, Bioassay, Biocompatibility, biomaterial, Biomaterials, biomineralization, bone marrow cell, bone mineralization, Bovinae, Calcification, Physiologic, Calcium, calcium phosphate, Carbon, Cations, Cattle, Cell Division, cell proliferation, cell survival, cell viability, Cells, Cultured, Chromium, controlled study, Corrosion, dexamethasone, electrochemistry, enzyme activity, Enzyme kinetics, glycerol 2 phosphate, histochemistry, Iron, Kinetics, manganese, metal ion, Metal ions, Metals, molybdenum, Nickel, nonhuman, Oryctolagus cuniculus, osteoblast, Osteoblast cells, Osteoblast-like cells, Osteoblasts, Phosphates, priority journal, rabbit, Rabbits, serum, Silicon, Stainless steel, Steel corrosion, Tetrazolium Salts, Thiazoles, Tissue culture},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031812308\&partnerID=40\&md5=946525340a0c386c984ce614d56ae081},
	author = {Morais, S. and Sousa, J.P. and Fernandes, M.H. and Carvalho, G.S.}
}
@article {2593,
	title = {In vitro biomineralization by osteoblast-like cells II. Characterization of cellular culture supernatants},
	journal = {BiomaterialsBiomaterials},
	volume = {19},
	year = {1998},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(97)00150-6PubMed ID: 9678846Language of Original Document: EnglishCorrespondence Address: Carvalho, G.S.; IEC, Universidade do Minho, Largo do Paco, 4719 Braga Codex, PortugalChemicals/CAS: Calcium, 7440-70-2; Chromium, 7440-47-3; Culture Media; Iron, 7439-89-6; Metals; Nickel, 7440-02-0; Phosphorus, 7723-14-0; Stainless Steel, 12597-68-1References: Pereira, M.L., Silva, A., Tracana, R., Carvalho, G.S., Toxic effects caused by stainless steel corrosion products on mouse seminiferous cells (1994) Cytobios, 77, pp. 73-80;Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Chromium accumulation and ultrastructural changes in the mouse liver caused by stainless steel corrosion products (1995) J Mater Sci Mater Med, 6, pp. 523-527; 
Tracana, R.B., Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Stainless steel corrosion products cause alterations on mouse spleen cellular populations (1995) J Mater Sci Mater Med, 6, pp. 56-61; 
Bravo, I., Sousa, S.R., Carvalho, G.S., Barbosa, M., Metals electrochemically dissolved in human serum inhibit T-lymphocyte function (1992) Advances in Biomaterials, pp. 7-11. , Doherty PJ, et al., editors. Amsterdam: Elsevier; 
Carvalho, G.S., Castanheira, M., Diogo, I., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1995) J Mater Sci Mater Med, 7, pp. 77-83; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro bone formation in the presence of stainless steel corrosion products (1994) Poster Section, 11th European Conference on Biomaterials, , Pisa, Italy; 
Sousa, J.P., Requirements in materials for biosensors J Mater Sci Mater Med, , in press; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralisation by osteoblast like cells. I. Retardation of tissue mineralisation by metal salts (1998) Biomaterials, 19, pp. 13-21; 
Morais, S., Carvalho, G.S., Sousa, J.P., Determination of nickel in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with mercury microelectrodes (1997) Electroanalysis, 9 (5), pp. 422-426; 
Morais, S., Carvalho, G.S., Sousa, J.P., Optimization study for nickel quantification in cell culture medium using mercury film microelectrodes (1996) Portugaliae Electrochimica Acta, 14, pp. 267-272; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9, pp. 791-795; 
Morais, S., Carvalho, G.S., Sousa, J.P., Chromium determination in osteoblast-like cell culture medium by catalytic stripping voltammetry with a mercury microelectrode Electroanalysis, , submitted for publication; 
Morais, S., Carvalho, G.S., Sousa, J.P., Potentiometric determination of total and ionised calcium in osteoblast-like cell culture medium (1996) Electroanalysis, 8 (12), pp. 1174-1178; 
Morais, S., Carvalho, G.S., Sousa, J.P., Inorganic phosphorus determination in osteoblast-like cell culture for in vitro mineralisation study (1996) Poster Section, 15th Meeting of the Portuguese Chemical Society, , Porto; 
Pend, J., Jin, W., Anodic stripping voltammetric determination of trace lead in micro-samples with a mercury ultramicroelectrode (1992) Anal Chim Acta, 264, pp. 213-219; 
Pereira, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using mercury microelectrodes (1997) Electroanalysis, 9, p. 1501; 
Wurfels, W., Jackwerth, E., Stoeppler, M., Residues from biological materials after pressure decomposition with nitric acid. Part 3a (1989) Anal Chim Acta, 226, pp. 31-41; 
Wurfels, W., Jackwerth, E., Stoeppler, M., Residues from biological materials after pressure decomposition with nitric acid. Part 2a (1989) Anal Chim Acta, 226, pp. 17-30; 
Kotz, L., Henze, G., Kaiser, G., Pahlke, S., Veber, R., Tolg, G., Wet mineralisation of organic matrices in glassy carbon vessels in a pressure-bomb system for trace element analysis (1979) Talanta, 26, pp. 681-691; 
Nakamoto, K., (1970) Infrared Spectra of Inorganic and Coordination Compounds, 2nd Edn., , NY: Wiley Interscience; 
Lin-Vien, D., Colthup, N.B., Fateley, W.G., Grasselli, J.G., (1991) The Handbook of Infrared and Raman Characteristic Frequencies of Organic Molecules, , Boston: Academic Press; 
Walsh, W.R., Christiansen, D.L., Demineralized bone matrix as a template for mineral-organic composites (1995) Biomaterials, 16 (18), pp. 1363-1371; 
Koeneman, J., Lemons, J., Ducheyne, P., Lacefield, W., Magee, F., Calahan, K.J., Workshop on characterisation of calcium phosphate materials (1990) J Appl Biomater, 1, pp. 79-90; 
Mann, S., Webb, J., Williams, R.J.P., (1989) Biomineralization: Chemical and Biochemical Perspectives, , Germany: VCH, Weinheim; 
Evans, E.M., Freeman, M.A.R., Miller, A.J., Vernon-Roberts, B., Metal sensitivity as a cause of bone necrosis and loosening of the prosthesis in total joint replacement (1974) J Bone Jt Surg, 566, pp. 626-642; 
De Vernejoul, M.C., Pointillart, A., Golenzer, C.C., Metals in bone (1985) Lancaster, pp. 321-331. , Priest ND, editor. MTT; 
Kusec, R., Nikolic, V., Hancevic, J., Davila, S., Alkovic, V., Trace elements in periosteal callus and cortical bone after plating for fracture (1990) Periodicum Biologorum, 92 (3), pp. 325-327; 
Betts, F., Wright, T., Salvati, E.A., Boskey, A., Mansal, M., Cobalt alloy metal debris in periarticular tissues from total hip revision arthoplasties (1992) Clin Orthop, 278, pp. 75-82; 
Brien, W.W., Salvati, E.A., Betts, F., Metal levels in cemented total hip arthroplasty: A comparison of well-fixed and loose implants (1992) Clin Orthop, 276, pp. 66-74
},
	month = {1998///},
	pages = {23 - 29},
	publisher = {Elsevier Sci Ltd},
	abstract = {The quantification of total calcium, phosphorus, iron, chromium and nickel in cell culture medium by electrochemical or spectroscopic means may require digestion of samples. Nevertheless, when pH adjustment is performed for values higher than about 6.5, the formation of two phases occurs: a white precipitate and a clear solution. Analysing both phases using microelectrodes, atomic absorption spectrometry (AAS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy. X-ray dispersive (XRD) analysis, scanning electron microscopy (SEM) and energy dispersive spectroscopic (EDS) analysis, it was oberved that iron, chromium and nickel are not co-precipitating with the white solid phase. If quantification of calcium, phosphorus and magnesium is intended, a ten-fold dilution at least, must be performed to avoid most of these elements going into the precipitate. This knowledge is crucial if a mineralization study is going to be made.The quantification of total calcium, phosphorus, iron, chromium and nickel in cell culture medium by electrochemical or spectroscopic means may require digestion of samples. Nevertheless, when pH adjustment is performed for values higher than about 6.5, the formation of two phases occurs: a white precipitate and a clear solution. Analyzing both phases using microelectrodes, atomic absorption spectrometry (AAS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, X-ray dispersive (XRD) analysis, scanning electron microscopy (SEM) and energy dispersive spectroscopic (EDS) analysis, it was observed that iron, chromium and nickel are not co-precipitating with the white solid phase. If quantification of calcium, phosphorus and magnesium is intended, a ten-fold dilution at least, must be performed to avoid most of these elements going into the precipitate. This knowledge is crucial if a mineralization study is going to be made.
},
	keywords = {Absorption spectroscopy, Animals, article, atomic absorption spectrometry, Biomaterials, bone mineralization, Calcification, Physiologic, Calcium, Cell culture medium, Cells, Cultured, Chromium, Culture Media, culture medium, Diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, electrochemistry, Energy dispersive spectroscopic analysis, Fourier transform infrared spectroscopy, Hydrogen-Ion Concentration, infrared spectroscopy, Iron, Magnesium, Metals, microelectrode, Microelectrodes, Nickel, nonhuman, osteoblast, Osteoblast cells, Osteoblasts, pH effects, phosphorus, precipitation, Precipitation (chemical), priority journal, Rabbits, Scanning electron microscopy, Spectrophotometry, Atomic, Spectroscopic techniques, Spectroscopy, Stainless steel, supernatant, Supernatants, Tissue culture, X ray analysis, X ray dispersive analysis},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031841533\&partnerID=40\&md5=62f6b7869003505ce8adfdf96ca2a9f0},
	author = {Morais, S. and Carvalho, G.S. and Faria, J.L. and Gomes, H.T. and Sousa, J.P.}
}
@article {2592,
	title = {Semiautomated ultrasonographic measurement of fetal nuchal translucency using a computer software tool},
	journal = {Ultrasound in Medicine and BiologyUltrasound Med. Biol.},
	volume = {24},
	year = {1998},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: USMBAdoi: 10.1016/S0301-5629(97)00235-4PubMed ID: 9483771Language of Original Document: EnglishCorrespondence Address: Marques De Sa, J.; Instituto de Engenharia Biomedica, Pt. Coronel Pacheco 1, P-4050 Porto, Portugal; email: jmsa@garfield.fe.up.ptTradenames: Microsoft Visual C++, microsoft, United StatesManufacturers: microsoft, United StatesReferences: Jain, A.K., (1982) Fundamentals of Digital Image Processing, , New York: Prentice Hall International Editions;Neilson, J., Grant, A., Ultrasound in pregnancy (1989) A Guide to Effective Care in Pregnancy and Childbirth., pp. 48-56. , Enkin M, Keirse MJN, Chalmers I. eds. New York, London: Oxford University Press; 
Nicolaides, K.H., Azar, G., Byrne, D., Mansur, C., Marks, K., Fetal nuchal translucency: Ultrasound screening for chromosomal detects in first trimester of pregnancy (1992) BMJ, 304, pp. 867-869; 
Pandya, P.P., Brizot, M.L., Kuhn, P., Snijders, R.J.M., Nicolaides, K.H., First trimester fetal nuchal translucency thickness and risk for trisomies (1994) Obstet Gynecol, 84, pp. 420-423; 
Pandya, P.P., Kondylios, A., Hilbert, L., Snijders, R.J.M., Nicolaides, K.H., Chromosomal defects and outcome in 1015 fetuses with increased nuchal translucency (1995) Ultrasound Obstet Gynecol, 5, pp. 15-19; 
Pandya, P.P., Altman, D.G., Brizot, M.L., Pettersen, H., Nicolaides, K.H., Repeatability of measurement of fetal nuchal translucency thickness (1995) Ultrasound Obstet Gynecol, 5, pp. 334-337; 
Roberts, L.J., Bewley, S., Mackinson, A.M., Rodeck, C.H., First trimester fetal nuchal translucency: Problems of screening the general population (1995) Br J Obstet Gynaecol, 102, pp. 381-385; 
Zador, I.E., Salari, V., Sokol, R.J., Computer applications in fetal medicine (1994) The Fetus As A Patient, pp. 117-124. , Kurjak A, Chervenak FA, eds. London/ New York: Parthenon Publ
},
	month = {1998///},
	pages = {51 - 54},
	publisher = {Elsevier Sci Ltd},
	abstract = {Nuchal translucency (NT) thickness measurement has been recently proposed as a part of routine ultrasound scanning during the late first trimester of pregnancy, for the early screening of chromosomal abnormalities. Manual determination of NT is currently performed using electronic callipers placed by the operator in the middle of two echogenic fines displayed on the screen. Therefore, intraobserver and interobserver repeatability can be questioned. This paper presents a software tool that has been developed for achieving this goal in a semiautomatic way, improving the reproducibility of the method.Nuchal translucency (NT) thickness measurement has been recently proposed as a part of routine ultrasound scanning during the late first trimester of pregnancy, for the early screening of chromosomal abnormalities. Manual determination of NT is currently performed using electronic callipers placed by the operator in the middle of two echogenic lines displayed on the screen. Therefore, intraobserver and interobserver repeatability can be questioned. This paper presents a software tool that has been developed for achieving this goal in a semiautomatic way, improving the reproducibility of the method.
},
	keywords = {article, cervical spine, chromosome aberration, Chromosome Aberrations, Chromosome Disorders, Computer aided diagnosis, computer program, Computer software, computer system, Female, Fetal monitoring, Fetal nuchal translucency, fetus echography, first trimester pregnancy, Humans, Image analysis, Image Processing, Computer-Assisted, Image thresholding, Medical imaging, Neck, observer variation, Pregnancy, Pregnancy Trimester, First, priority journal, reproducibility, Reproducibility of Results, Software, Software package NUCA, Thickness measurement, Ultrasonic imaging, Ultrasonography, Prenatal, ultrasound},
	isbn = {03015629 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031974123\&partnerID=40\&md5=632da5e0661d27d445b101e03057ad56},
	author = {Bernardino, F. and Cardoso, R. and Montenegro, N. and Bernardes, J. and Marques de S{\'a}, J.}
}
@article {2607,
	title = {Iron Determination in Osteoblast-Like Cell Culture Medium by Adsorptive Cathodic Stripping Voltammetry with a Mercury Microelectrode},
	journal = {ElectroanalysisElectroanalysis},
	volume = {9},
	year = {1997},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: ELANELanguage of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Inst. de Engenharia Biomedica, Depto. de Engenharia Qu{\'\i}mica, Universidade do Porto, Pra{\c c}a Coronel Pacheco, 1, 4050 Porto, PortugalReferences: Cigada, A., (1994) Proc. Symp. Compatibility of Biomedical Implants, 94 (15), pp. 185-192. , (Eds.: P. Kovacs, N.S. Istephanous), The Electrochemical Society, Pennington, NJ;Pohler, O.E.M., (1983) Biomaterials in Reconstrutive Surgery, p. 158. , C.V. Mosby, St. Louis; 
Black, J., Oppenheimer, P., Morris, D.M., Peduto, A.M., Clark, C.C., (1987) J. Biomed. Mater. Res., 21, p. 1213; 
Sunderman, F.W., Hopper, S.M., Swift, T., Rezuke, W.N., Ziebra, L., Highman, P., Edwards, B., Gossling, H.R., (1989) J. Orthop. Res., 7, p. 307; 
Traisnel, M., Maguer, D., Hildebrand, H.F., (1990) Clin. Mater., 5, p. 309; 
Moore, R.M., Hamburger, S., Jeng, L.J., Hamilton, P.M., (1991) J. Appl. Biomater., 2, p. 127; 
Henning, E.F., Raithel, H.J., Schaller, K.H., Dohler, J.R., (1992) J. Trace Elem. Electrolytes Health Dis., 6, p. 239; 
Brown, S.A., Zhang, K., Merritt, K., Payer, J.H., (1993) J. Biomed. Mater. Res., 27, p. 1007; 
Albore-Saavedra, J., Vuitch, F., Delgado, R., Wiley, E., Hagler, H., (1994) Am. Surg. Pathol., 18, p. 83; 
Buechel, F.F., Drucker, D., Jasty, M., Jiranek, W., Harris, W.H., (1994) Clin. Orthop. Related Res., 298, p. 202; 
Valenta, P., Rutzel, H., Nurnberg, H.W., Stoeppler, M., (1977) Z. Anal. Chem., 285, p. 25; 
Nurnberg, H.W., (1977) Electrochim. Acta, 22, p. 935; 
Nurnberg, H.W., (1978) Proc. Anal. Div. Chem. Soc., 15, p. 275; 
Golimowski, S.J., Valenta, P., Stoepper, M., Nurnberg, H.W., (1979) Talanta, 26, p. 649; 
Cox, J.A., Majda, M., (1980) Anal. Chim. Acta, 118, p. 271; 
Van Den Berg, C.M.G., Huang, Z.Q., (1984) J. Electroanal. Chem., 177, p. 269; 
Wang, J., Mahmoud, J.S., (1986) J. Electroanal. Chem., 208, p. 383; 
Van Den Berg, C.M.G., Nimmo, N., Abollino, O., Mentasti, E., (1991) Eleclroanalysis, 3, p. 477; 
Sousa, J.P., Morais, S., Pereira, M.C., Pereira, M.L., Carvalho, G.S., Barbosa, M.A., (1994) Biomaterials, 15, p. 821; 
Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., (1995) J. Mater. Sci.: Mater. Med., 6, p. 523; 
Tracana, R.B., Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., (1995) J. Mater. Sci.: Mater. Med., 6, p. 56; 
Vilamitjana-Amedee, J., Bareille, R., Rouais, F., Caplan, A.I., Harmand, M.F., (1993) In Vitro Cell. Dev. Biol., 29 A, p. 699; 
Bizios, R., (1994) Biotechnol. Bioeng., 43, p. 582; 
Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Arciola, C.R., Granchi, D., Savarino, L., (1994) Clin. Mater., 15, p. 173; 
Winter, G.D., (1974) J. Biomed. Mater. Res. Symp., 5, p. 11; 
De Vernejoul, M.C., Pointillart, A., Golenzer, C.C., Morieux, C., Bielakoff, J., Modrowski, D., Miravet, L., (1985) Metals in Bone, pp. 321-331. , (Ed: N.D. Priest), MTT Press Dordrecht; 
Pend, J., Jin, W., (1992) Anal. Chim. Acta, 264, p. 213; 
Van Den Berg, C.M.G., (1984) Anal. Chim. Acta, 164, p. 195; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., (1988) Cell Tissue Res., 254, p. 317; 
Kotz, L., Henze, G., Kaiser, G., Pahlke, S., Veber, M., Tolg, G., (1979) Talanta, 26, p. 681; 
Huizenga, D.L., Rester, D.R., (1984) J. Electroanal. Chem., 164, p. 229; 
Capodaglio, G., Van Den Berg, C.M.G., Scarponi, G., (1987) J. Electroanal. Chem., 235, p. 275; 
Torrance, K., Gatford, C., (1986) Analyst, 111, p. 359; 
Wang, J., Lu, J., Olsen, K., (1992) Analyst, 117, p. 1913; 
Miller, J.N., (1991) Analyst, 116, p. 3; 
Morais, S., Sousa, J.P., Fernandes, H., Carvalho, G.S., (1994) Last Minute Posters Abstracts Book, 11th European Conference on Biomaterials, pp. 10-11. , Pisa, Italy; 
Lugowski, S., Smith, D.C., Van Loon, J.C., (1987) J. Biomed. Mater. Res., 21, p. 657
},
	month = {1997///},
	pages = {791 - 795},
	abstract = {Mercury film microelectrodes were used to determine iron in osteoblast-like cell culture medium by performing adsorptive cathodic stripping voltammetry in the presence of catechol. The optimized analytical conditions are a catechol concentration of 5.00{\texttimes}10},
	keywords = {Cell culture medium, Iron, Mercury film microelectrode},
	isbn = {10400397 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0001767651\&partnerID=40\&md5=b6108f6d2f2d7bbe73818c78d82a74eb},
	author = {Morais, S. and Carvalho, G.S. and Sousa, J.P.}
}
@article {2608,
	title = {Nickel Determination in Osteoblast-Like Cell Culture Medium by Adsorptive Cathodic Stripping Voltammetry with a Mercury Microelectrode},
	journal = {ElectroanalysisElectroanalysis},
	volume = {9},
	year = {1997},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: ELANELanguage of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Inst. de Engenharia Biomedica, Depto. de Engenharia Qu{\'\i}mica, Universidade do Porto, Pra{\c c}a Coronel Pacheco, 1, 4050 Porto, PortugalReferences: Williams, D.F., (1981) Biomaterials and Biocompatibility: An Introduction, in Fundamental Aspects of Biocompatibility, 1. , CRC Press, Boca Raton, FL;Tsalev, D.L., Zaprianov, Z.K., (1983) Atomic Absorption Spectrometry in Occupational and Environmental Health Practice, 1. , CRC Press, Boca Raton, FL; 
Fishbein, L., Mehlman, M.A., (1987) Advances in Modern Environmental Toxicology, pp. 145-183. , CRC Press, Boca Raton, FL; 
Costa, M., (1989) Env. Health. Perspect., 81, p. 73; 
Ward, J., Thornbury, D.D., Lemons, J.., Dunham, W.., (1990) Clin. Orthoped., 252, p. 299; 
Florence, T.M., (1970) J. Electroanal. Chem., 27, p. 273; 
Batley, J.E., Florence, T.M., (1976) J. Electroanal. Chem., 72, p. 121; 
Wang, J., Zadeii, Z., (1988) J. Electroanal. Chem., 246, p. 297; 
Daniele, S., Baldo, M.A., Ugo, P., Mazzocchin, G., (1989) Anal. Chim. Acta, 219, p. 9; 
Daniele, S., Baldo, M.A., Ugo, P., Mazzocchin, G., (1989) Anal. Chim. Acta, 219, p. 19; 
Eskilsson, H., Haraldsson, C., Jagner, D., (1985) Anal. Chim. Acta., 175, p. 79; 
Economou, A., Fielden, P.R., (1993) Analyst, 118, p. 47; 
Wightman, R.M., Wipt, D.O., (1989) Voltammetry at Ultramicroelectrodes, pp. 335-336. , Ed: A.J. Bard, Marcel Dekker, New York; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., (1995) Biomed. Lett., 52, p. 235; 
Sousa, J.P., Morais, S., Pereira, M.C., Pereira, M.L., Carvalho, G.S., Barbosa, M.A., (1994) Biomaterials, 15, p. 821; 
Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., (1995) J. Mater. Sci.: Mater. Med., 6, p. 523; 
Tracana, R.B., Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., (1995) J. Mater. Sci.: Mater. Med., 6, p. 56; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., (1988) Cell Tiss. Res., 254, p. 317; 
Morais, S., Sousa, J.P., Fernandes, H., Carvalho, G.S., (1994) Last Minute Posters Abstracts Book, 11th European Conference on Biomaterials, Pisa, Italy, pp. 10-11; 
Peng, J., Jin, W., (1992) Anal. Chim. Acta, 264, p. 213; 
Gunawardena, G., Hills, G., Montenegro, I., Scharifker, B., (1982) J. Electroanal. Chem., 138, p. 225; 
Milchev, A., Scharifker, B., Hills, G., (1981) J. Electroanal. Chem., 132, p. 277; 
Sousa, J.P., (1991), Ph.D. Dissertation, University of UtahBailey, F., Malinski, T., Kiechle, F., (1991) Anal. Chem., 63, p. 395; 
Wightman, R.M., (1981) Anal. Chem., 53, pp. 1125A; 
Turner, A.P.F., Karube, I., Wilson, G.S., (1987) Biosensors Fundamentals and Applications, , Oxford University Press, Oxford; 
Golas, J., Galus, Z., Osteryoung, J., (1987) Anal. Chem., 59, p. 389; 
Baranski, A.S., (1987) Anal. Chem., 59, p. 662; 
Kounaves, S.P., (1992) Anal. Chem., 64, p. 2998; 
Feinberg, J.S., Bowyer, W.J., (1993) Microchem. J., 47, p. 72; 
Kounaves, S.P., Deng, W., (1993) Anal. Chem., 65, p. 375; 
Bustin, D., Mesaros, S., Rievaj, M., Tomcik, P., (1995) Electroanalysis, 4, p. 7; 
Ostapczuk, P., Valenta, P., Nurnberg, H., (1986) J. Electroanal. Chem., 214, p. 51; 
Pihlar, B., Valenta, P., Nurnberg, H.W., (1981) Z. Anal. Chem., 307, p. 337; 
Gammelgaar, B., Andersen, J.R., (1985) Analyst, 110, p. 1197; 
Vos, L., Komy, Z., Reggers, G., Rockens, E., Van Grieken, R., (1986) Anal. Chim. Acta, 184, p. 271; 
Torrance, K., Gatford, C., (1985) Talanta, 32, p. 273; 
Braun, H., Metzgar, M., (1984) Z. Anal. Chem., 318, p. 321; 
Adeloju, S.B., Bond, A.M., Briggs, M.H., (1984) Anal. Chim. Acta, 164, p. 181; 
Wahdat, F., Neeb, R., (1985) Z. Anal. Chem., 320, p. 334; 
Kotz, L., Henze, G., Kaiser, G., Pahlke, S., Veber, R., Tolg, G., (1979) Talanta, 26, p. 681; 
Wurfels, M., Jackwerth, E., Stoeppler, M., (1989) Anal. Chim. Acta, 226, p. 17; 
Wurfels, M., Jackwerth, E., Stoeppler, M., (1989) Anal. Chim. Acta, 226, p. 31; 
Basu, B.J., Rajagopalan, S.R., (1992) Analyst, 117, p. 1623; 
Bond, A.M., Luscombe, D.L., (1986) J. Electroanal. Chem., 214, p. 21; 
Van Den Berg, C.M.G., (1991) Anal. Chim. Acta, 250, p. 265; 
Miller, J.N., (1991) Analyst, 116, p. 3; 
Wu, T.G., Wong, J.L., (1991) Anal. Chim. Acta, 246, p. 301; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., (1991) Bone and Mineral, 14, p. 27; 
Wehmeyer, K.R., Wightman, R.M., (1985) Anal. Chem., 57, p. 1989; 
Nurnberg, H.W., (1977) Electrochim. Acta, 22, p. 935
},
	month = {1997///},
	pages = {422 - 426},
	abstract = {The purpose of this study is to investigate the influence of nickel, which is an alloying element in commonly used metallic biomaterials, on the biomaterials mineralization process. An electrochemical method was developed to quantify this metal ion in osteoblast-like cell culture medium (OST) by performing adsorptive cathodic stripping voltammetry (CSV) with dimethylglyoxime (DMG) at a mercury film microelectrode (MFM). The optimized analytical conditions and the square-wave CSV parameters for the analysis are: DMG concentration: 5.00 {\texttimes} 10},
	keywords = {Cell culture medium, Mercury film microelectrode, Nickel},
	isbn = {10400397 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0010434908\&partnerID=40\&md5=2547683b89d88a2385cea47a77bed5e8},
	author = {Morais, S. and Carvalho, G.S. and Sousa, J.P.}
}
@article {2595,
	title = {Processing and in vitro degradation of starch/EVOH thermoplastic blends},
	journal = {Polymer InternationalPolym. Int.},
	volume = {43},
	year = {1997},
	note = {Cited By (since 1996): 77Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Reis, R.L.; Dept. of Metallurgical Engineering, University of Porto, FEUP, Rua dos Bragas, 4099 Porto Codex, PortugalReferences: Hayashi, T., (1994) Prog. Polym. Sci., 19, p. 663;Daniels, A.U., Andriano, K.P., Smutz, W.P., Chang, M.K.O., Heller, J., (1994) J. Appl. Biomater., 5, pp. 5l; 
Andriano, K.P., Pohjonen, T., Tormalla, P., (1994) J. Appl. Biomater., 5, p. 133; 
Knowles, J.C., Hastings, G.W., (1992) J. Mater. Sci.: Mater. Med., 3, p. 352; 
Rokkanen, P.U., (1991) Ann. Med., 23, p. 109; 
Reis, R.L., Cunha, A.M., (1995) J. Mater, Sci.: Mater. Med., 6, p. 786; 
Reis, R.L., Cunha, A.M., Allan, P.S., Bevis, M.J., (1996) J. Polym. Advanced Techniques, 1, p. 784; 
Bastioli, C., Bellotti, V., Del Giudice, L., Lombi, R., (1991) PCT Int. Pat. Appl, , WO91/02025; 
Bhattarcharya, M., Vaidya, U.R., Zhang, D., Narayan, R., (1995) J. Appl Polym. Sci., 57, p. 539; 
Bastioli, C., Bellotti, V., Del Guidice, L., Gilli, G., (1993) J. Environ. Polym. Degrad., 1, p. 181; 
Bastioli, C., Bellotti, V., Rallis, A., (1994) Rheol. Acta., 33, p. 307; 
Griffin, G.J.L., (1994) Polym. Degrad. Stabil, 45, p. 241; 
Ogbona, G.L., Kalay, G., Allan, P.S., Bevis, M.J., (1995) J. Appl. Polym. Sci., 58, p. 213; 
Allan, P.S., Bevis, M.J., (1987), Br. Pat., 2170-140-BAllan, P.S., Bevis, M.J., (1987) Plast. Rubb. Process. Applic., 1, p. 3; 
Allan, P.S., Bevis, M.J., (1990) Comp. Manuf., 2, p. 79; 
Reis, R.L., Cunha, A.M., Allan, P.S., Bevis, M.J., (1996) Polymers in Medicine and Surgery, p. 195. , eds J. Courtney et al. Institute of Materials, London; 
Bastioli, C., Bellotti, V., Camia, M., Del Giudice, L., Rallis, A., (1994) Biodegradable Plastics and Polymers, p. 200. , eds Y. Doi and K. Fukuda. Eisevier, Amsterdam
},
	month = {1997///},
	pages = {347 - 352},
	abstract = {This paper describes the processing dependence of the mechanical properties of three blends of starch/ethylene vinyl alcohol (EVOH) with potential uses as biomaterials. These blends exhibit a distinct rheological behaviour and mechanical performance. Using shear controlled orientation in injection moulding (Scorim) it was possible to induce anisotropy into the mouldings and to simultaneously enhance stiffness and ductility. Degradation was studied in simulated physiological solutions (Hank{\textquoteright}s balanced salt solution) with and without added bovine serum. Both the dry weight loss and the changes in mechanical properties were determined for ageing periods up to 80 days. The degradation behaviour proved to be strongly dependent on the formulation of the material studied, and on the addition of proteins. The susceptibility of the starch/EVOH blends to degradation when sterilised with ethylene oxide (EtO) was also studied, and showed that the polymers could stand one EtO sterilisation cycle. However, two consecutive cycles severely degraded the polymer structure and properties.},
	keywords = {Aging of materials, Anisotropy, Biodegradable polymers, Biodegradation, Biomaterials, Degradation, Ductility, Ethylene oxide, Ethylene vinyl alcohol, Hank{\textquoteright}s salt solution, Injection molding, Polyethylenes, Polymer blends, Polyvinyl alcohols, Processing, Proteins, rheology, Shear controlled orientation injection molding (Scorim), Starch, Sterilisation, Stiffness},
	isbn = {09598103 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031212625\&partnerID=40\&md5=554d54fa82a40d6b0a6dea9b9c12afa2},
	author = {Reis, R.L. and Mendes, S.C. and Cunha, A.M. and Bevis, M.J.}
}
@article {2615,
	title = {Amino acid and metal content of Crassostrea gigas shell infested by Polydora sp. in the prismatic layer insoluble matrix and blister membrane},
	journal = {Aquatic Living ResourcesAquatic Living Resour.},
	volume = {9},
	year = {1996},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: ALREELanguage of Original Document: EnglishCorrespondence Address: Almeida, M.J.; Laborat{\'o}rio de Fisiologia Aplicada, Instituto de Ci{\^e}ncias Biom{\'e}dicas Abel Salazar, 4050 Porto, PortugalReferences: Allen, J.A., Manganese deposition on the shell of living molluscs (1960) Nature, 185, pp. 336-337;Beedham, G.E., Repair of the shell in species of Anodonta (1965) Proc. Zool. Soc. London, 145, pp. 107-125; 
Boggess, R.K., Martin, R.B., Copper chelation by DOPA, epinephrine and other catechols (1975) J. Am. Soc. Chem., 97, pp. 3076-3081; 
Brown, C.H., (1975) Structural Materials in Animals, , Wiley, New York; 
Bubel, A., An electron microscope study of periostracum repair in Mytilus edulis (1973) Mar. Biol., 20, pp. 235-244; 
Carriker, M.R., Swann, C.P., Prezant, R.S., Counts, C.L., Chemical elements in the aragonitic and calcitic microstructural groups of shell of the oyster Crassostrea virginica: A proton probe study (1991) Mar. Biol., 109, pp. 289-297; 
Catherine, M., Blateau, D., Mazuri{\'e}, J., Le Bec, C., (1990) Anomalies des Coquilles D{\textquoteright}hu{\^\i}tres Creuses Crassostrea Gigas Observ{\'e}es sur le Littoral Fran{\c c}ais en Mai-juin 1989, Dues au ver Polydora et aux Peintures Antisalissures., pp. 13-14. , Rapp. Int. Dir. Ressources Vivantes, IFREMER Nantes; 
Degens, E.T., Spencer, D.W., Parker, R.H., Paleobiochemistry of Molluscan shell proteins (1967) Comp. Biochem. Physiol., 79, pp. 553-579; 
Dorsett, D.A., The behavior of Polydora ciliata (Johnst.). Tube building and burrowing (1961) J. Mar. Biol. Assoc. U.K., 41, pp. 577-590; 
Felix, C.C., Sealy, R.C., Electron spin resonance characterization of radicals from 3-4-dihydroxyphenylalanine: Semiquinone anions and their metal chelates (1981) J. Am. Chem. Soc., 103, pp. 2831-2835; 
Galstoff, P.S., The American oyster Crassostrea virginica (Gmelin) (1964) Fish. Bull. U.S., p. 64; 
Gergely, A., Kiss, T., Coordination chemistry of L-DOPA and related ligands (1979) Met. Ions Biol. Syst., 9, pp. 143-172; 
Haigler, S., Boring mechanism of Polydora websteri inhabiting Crassostrea virginica (1969) Am. Zool., 9, pp. 821-828; 
Jones, G.M., Saleuddin, A.S.M., Cellular mechanisms of periostracum formation in Physa spp. (Mollusca: Pulmonata) (1978) Can. J. Zool., 56, pp. 2299-2311; 
Kawaguchi, T., Watabe, N., The organic matrices of the shell of the American oyster Crassostrea virginica Gmelin (1993) J. Exp. Mar. Biol. Ecol., 170, pp. 11-28; 
Kawaguti, S., Ikemoto, N., Electron microscopy on the mantle of a bivalve Musculus senhousia, during regeneration of shell (1962) Biol. J. Okayama Univ., 8, pp. 31-42; 
Krampitz, G., Drolshagen, H., Hotta, S., Simultaneous binding of calcium and bicarbonate by conchiolin of oyster shells (1983) Experiemia, 39, pp. 1104-1105; 
Laukner, G., Diseases of Mollusca: Bivalvia (1983) Diseases of Marine Animals, Vol. II, Bivalvia to Scaphopoda, 2, pp. 477-978. , Biologische Anstalt Helgoland, O. Kinne ed. Hamburg; 
Levitt, M., A simplified representation of protein conformation for rapid simulation of protein folding (1976) J. Mol. Biol., 104, pp. 59-108; 
Linderstrom-Lang, K., Duspiva, F., Studies in enzymatic histochemistry. XVI. the digestion of keratin by larvae of clothes moth (Tineola biselliela Humm.) (1936) C. R. Trav. Lab. Carlsberg, Ser. Chim., 21, pp. 1-82; 
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein determination with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; 
Martius, K.V., Schwarzhans, K.E., Preparation of the Mn (II) complex with 3,4-dihydroxyphenylalanine (DOPA) (1978) Z Naturforsch., 33 B, p. 124; 
Meenaakshi, V.R., Hare, P.E., Watabe, N., Wilbur, K.M., The chemical composition of the periostracum of the molluscan shell (1969) Comp. Biochem. Physiol., 29, pp. 611-620; 
Nozaki, Y., Tanford, C., The solubility of amino acids and two glycyl peptides in aqueous ethanol and dioxane solutions (1971) J. Biol. Chem., 246, pp. 2211-2217; 
Saleuddin, A.S.M., Petit, H.P., The mode of formation and the structure of the periostracum (1983) The Mollusca, 4, pp. 199-234. , K. M. Wilbur ed. Academic Press, New York; 
Sato-Okoshi, W., Okoshi, K., Microstructure of scallop and oyster shells infested with boring Polydora (1993) Nippon Suisan Gakkaishi, 59, pp. 1243-1247; 
Segar, D.A., Collins, J.D., Riley, J.P., The distribution of the major and some minor elements in marine animals. Part II (1971) Molluscs. J. Mar. Biol. Assoc. U.K., 51, pp. 131-136; 
Swinehart, J.H., Smith, K.W., Iron and manganese deposition in the periostraca of several bivalve molluscs (1979) Biol. Bull., 156, pp. 369-381; 
Travis, D.F., Gonsalves, M., Comparative ultrastructure and organization of the prismatic region of two bivalves and its possible relation to the chemical mechanism of boring (1969) Am. Zool., 9, pp. 635-661; 
Tsujii, T., An electron microscopic study of the mantle epithelial cells of Anodonta sp. during shell regeneration (1976) The Mechanisms of Mineralization in the Invertebrates and Plants, pp. 339-353. , N. Watabe, K. M. Wilbur eds. Univ. South Carolina Press, Columbia; 
Vovelle, J., Scl{\'e}rotisation et min{\'e}ralisation des structures squellettiques chez les Mollusques (1974) Haliotis, 2, pp. 133-165; 
Waite, J.H., Evidence for the mode of sclerotization in a molluscan periostracum (1977) Comp. Biochem. Physiol., 58 B, pp. 157-162; 
Waite, J.H., Quinone-tanned scleroproteins (1983) The Mollusca, 1, pp. 467-504. , K. M. Wilbur ed. Academic Press, New York; 
Waite, J.H., Andersen, S.O., 3,4-dihydroxyphenyl-alanine in an insoluble shell protein of Mytilus edulis (1978) Biochem. Biophys. Acta, 541, pp. 107-114; 
Wheeler, A.P., Rusenko, K.W., Swift, D.M., Sikes, C.S., Regulation of in vitro and in vivo CaCO 3 crystallization by fractions of oyster shell organic matrix (1988) Mar. Biol., 98, pp. 71-80; 
Zottoli, R.A., Carriker, M.R., Burrow morphology, tube formation and microarchitecture of shell dissolution by the spionid polychaete Polydora websteri (1974) Mar. Biol., 27, pp. 307-316
},
	month = {1996///},
	pages = {179 - 186},
	abstract = {The spionid polychaete Polydora sp. can live and reproduce inside the oysters{\textquoteright} shell, excavating a U-shaped burrow the central portion of which is filled with detritus and particles of dissolved shell. The oyster responds by secreting an organic layer, confining the worm, and later covering it with calcitostracum. This process creates a characteristic "mud blister". Crassostrea gigas were analysed for the organic matrix amino acid content and 14 metals of the whole shell of normal oysters and severely Polydora infested ones. Amino acid, DOPA and FT-Raman analyses were also performed on isolated membranes from the characteristic mud blister and on prismatic layer insoluble matrix (PLIM) of infested oysters, with the aim of detecting any differences between the two organic matrices. Membranes and PLIM composition differ from whole normal shell organic matrix by having lower aspartate and glycine and higher levels of virtually all hydrophobic amino acids, especially alanine. There are no significant differences between membranes and PLIM in the ratio of charged to non-polar amino acids, respectively 0.54 and 0.59. L-DOPA content is also similar in the two matrices with 0.45 ({\textpm}0.18) and 0.57 ({\textpm}0.30) ng.mg},
	keywords = {Amino acids, Crassostrea gigas, Metals, Polydora sp., Shell organic matrix},
	isbn = {09907440 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0008495129\&partnerID=40\&md5=80ed4fe02d098797dec36a0481fbbecf},
	author = {Almeida, M.J. and Moura, G. and MacHado, J. and Coimbra, J. and Vilarinho, L. and Ribeiro, C. and Soares-Da-Silva, P.}
}
@article {2621,
	title = {Crystallinity and structural changes in HA plasma-sprayed coatings induced by cyclic loading in physiological media},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {7},
	year = {1996},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00122009Language of Original Document: EnglishCorrespondence Address: Reis, R.L.; Dept. of Metallurgical Engineering, FEUP, Univ. of Porto, Rua dos Bragas, 4099 Porto Codex, PortugalReferences: Ji, H., Marquis, P.M., (1993) Biomaterials, 14, p. 64;Pfaff, H.G., Willmann, G., Henne, R., (1993) Proceedings of the 10th European Conference on Biomaterials, p. 110. , Davos, Switzerland, 1993, edited by B. Rahn (European Society for Biomaterials); 
Brown, S.D., (1984) Thin Solid Films, 119, p. 127; 
Geesink, R.G.T., (1988), PhD thesis, Univ. Leiden, The NetherlandsVan Blitterswijk, C.A., Grote, J.J., Kuypers, W., Blok-Van Hoek, C.J.G., Daems, W.Th., (1985) Biomaterials, 6, p. 243; 
Ribeiro, C.C., Barbosa, M.A., (1991) Bioceramics 4, p. 145. , edited by W. Bonfield, G. W. Hastings and K. Tanner (Butterworth-Heinemann London); 
Ducheyne, P., Healy, K.E., (1988) J. Biomed. Mater. Res., 22, p. 1137; 
Whitehead, R.Y., Lucas, L.C., Lacefield, W.R., (1993) Clin. Mater., 12, p. 31; 
De Bruijn, J.D., Davies, J.E., Flach, J.S., De Groot, K., Van Blitterswijk, C.A., (1992) Proceedings of the 4th World Congress on Biomaterials - Workshop on Biomaterials and Tissue Interactions, p. 23. , edited by U. Gross (Berlin); 
Morita, M., Sasada, T., Hayashi, H., Tsukamoto, Y., (1988) J. Biomed. Mater. Res., 22, p. 529; 
Brown, S.A., Farsnworth, L.J., Merritt, K., Crowe, T.D., (1988) J. Biomed. Mater. Res., 22, p. 321; 
Kummer, F., Jaffe, W., (1992) J. Appl. Biomater., 3, p. 211; 
Reis, R.L., Monteiro, F.J., Garrido, A., (1992) Bioceramics 5, p. 25. , Kyoto, November, 1992, edited by T. Yamamura, T. Kokubo and T. Nakamura (Kobunshi Kankokai, Tokyo); 
Reis, R.L., Monteiro, F.J., (1993) Bioceramics 6, p. 413. , Philadelphia, USA, 1993, edited by P. Ducheyne and D. Christiansen (Butterworth-Heinemann. New York); 
Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) J. Mater. Sci. Mater. Med., 5, p. 457; 
Reis, R.L., Monteiro, F.J., (1995) Proceedings of the NATO/ASI on Materials Science and Implant Orthopaedic Surgery II, p. 163. , Chania, Greesce, June 1994. edited by R. Kossowsky and N. Kossowsky (May, NATO/ASI series E: Applied Sciences, Kluwer Press, Amsterdam); 
Ji, H., Ponton, C.B., Marquis, P.M., (1992) J. Mater. Sci. Mater. Med., 3, p. 283; 
Weng, J., Liu, X., Zhang, X., Ma, Z., Ji, X., Zyman, Z., (1993) Biomaterials, 14, p. 578; 
De Groot, K., Klein, C.A.P.T., Wolke, J.G.C., Blieck-Hogervorst, J.M.A., (1990) Handbook of Bio-active Ceramics, 2, p. 133. , edited by T. Yamamuro, L. L. Hench and J. Wilson (CRC Press, Boca Raton, FL); 
Del{\'e}crin, J., Szumuckler-Moncler, S., Daculsi, G., Rieu, J., Duquet, B., (1991) Bioceramics 4, p. 311. , London, September, 1991, edited by W. Bonfield, G. W. Hastings and K. E. Tanner (Butterworth-Heinemann, London); 
De Bruijn, J.D., Flach, J.S., Leenders, H., Van der Brink, J., Van Blitterswijk, C., (1992) Bioceramics 5, p. 259. , Kyoto, November, 1992, edited by T. Yamamura, T. Kokubo and T. Nakamura (Kobunshi Kankokai, Tokyo)
},
	month = {1996///},
	pages = {407 - 411},
	publisher = {Chapman \& Hall Ltd},
	abstract = {In this study hydroxyapatite plasma-sprayed Ti-6A1-4V was characterized by X-ray diffraction (XRD) after being cyclically loaded, while immersed in two different testing environments: Hank{\textquoteright}s balanced salt solution (HBSS) and an isotonic saline solution (0.15m NaCl). An original method was developed to test these systems under cyclic bending, while immersed in the testing solution. The crystallinity of the coating was calculated, before and after testing. Crystallinity changes were related to coating dissolution rates, measured by atomic absorption spectroscopy (AAS) and by a photocolourimetric method using ascorbic acid. Structural changes induced either by the plasma-spraying procedure or due to corrosion-fatigue tests, namely a preferential dissolution of the coating{\textquoteright}s amorphous phase, were also found in X-ray diffraction patterns. Long-term immersion tests, without any applied load, were carried out for assessing cyclic loading effects. An immersion for 2 years in static conditions was found to be equivalent to a cyclic test of 27.8 h in the same solution, as measured by the changes on the coating structure and by the amount of coating material lost to the solution.In this study hydroxyapatite plasma-sprayed Ti-6Al-4V was characterized by X-ray diffraction (XRD) after being cyclically loaded, while immersed in two different testing environments: Hank{\textquoteright}s balanced salt solution (HBSS) and an isotonic saline solution (0.15M NaCl). An original method was developed to test these systems under cyclic bending, while immersed in the testing solution. The crystallinity of the coating was calculated, before and after testing. Crystallinity changes were related to coating dissolution rates, measured by atomic absorption spectroscopy (AAS) and by a photocolourimetric method using ascorbic acid. Structural changes induced either by the plasma-spraying procedure or due to corrosion-fatigue tests, namely a preferential dissolution of the coating{\textquoteright}s amorphous phase, were also found in X-ray diffraction patterns. Long-term immersion tests, without any applied load, were carried out for assessing cyclic loading effects. An immersion for 2 years in static conditions was found to be equivalent to a cyclic test of 27.8 h in the same solution, as measured by the changes on the coating structure and by the amount of coating material lost to the solution.
},
	keywords = {Absorption spectroscopy, article, ascorbic acid, atomic absorption spectrometry, Atomic absorption spectroscopy, balanced salt solution, Bending (deformation), Calculations, Characterization, Corrosion fatigue tests, crystal structure, Crystallinity, Cyclic bending, Dissolution, Fatigue testing, Hanks balanced salt solution, hydroxyapatite, Hydroxyapatite plasma sprayed coatings, immersion, implant, Isotonic saline solution, loading test, Long term immersion tests, materials testing, Modification, Photocolourimetric method, photometry, Plasma spraying, priority journal, sodium chloride, Sprayed coatings, Titanium alloys, Titanium aluminum vanadium alloys, X ray crystallography, X ray diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030199724\&partnerID=40\&md5=b47480a26cc5b628bc98bc98034c6c98},
	author = {Reis, R.L. and Monteiro, F.J.}
}
@conference {2614,
	title = {Fracture toughness of hydroxyapatite reinforced with P                    2O
                    5-CaO glasses containing Na
                    2O, K
                    2O and MgO
},
	booktitle = {Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials SymposiumTrans Annu Meet Soc Biomater Int Biomater Symp},
	series = {Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2)},
	volume = {2},
	year = {1996},
	note = {Conference code: 45651Export Date: 21 November 2012Source: ScopusCODEN: TAMSELanguage of Original Document: EnglishCorrespondence Address: Silva, P.L.; INEB, Porto, Portugal},
	month = {1996///},
	pages = {58},
	publisher = {Soc for Biomaterials},
	organization = {Soc for Biomaterials},
	address = {Toronto, Can},
	abstract = {Calcium phosphate materials are widely used in biomedical applications but their poor strength and brittleness limit their use in load bearing applications. To improve their mechanical properties, hydroxyapatite (HA) ceramics were reinforced with P                        2O
                        5-CaO based glasses with additions of Na
                        2+, Mg
                        2+ and K
                        + oxides. A composite chemical composition that approaches the inorganic part of bone chemical composition was obtained using HA with calcium phosphate-based glasses. Fracture toughness was significantly enhanced by glass additions, and the decrease in fracture toughness at 1300 and 1350 {\textdegree}C was attributed to grain growth since it significantly increased with sintering temperature.
},
	keywords = {Biomaterials, Bone, Composition effects, Fracture toughness, glass, Grain growth, hardness, Hydroxyapatites, Lime, Magnesia, Phosphate minerals, Potassium compounds, Sodium compounds, Thermal effects},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030409631\&partnerID=40\&md5=d9b6e11fdb55db7fe76d61b3cb90b928},
	author = {Silva, P.L. and Santos, J.D. and Knowles, J.C. and Silva, R.F. and Monteiro, F.J.},
	editor = {Anon}
}
@article {2623,
	title = {In vitro calcium phosphate formation on SiO                    2-Na
                    2O-CaO-P
                    2O
                    5 glass reinforced hydroxyapatite composite: A study by XPS analysis
},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {7},
	year = {1996},
	note = {Cited By (since 1996): 42Export Date: 21 November 2012Source: ScopusCODEN: JSMMELanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; FEUP, Rua dos Bragas, 4099-Porto Codex, PortugalChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; hydroxyapatite, 1306-06-5, 51198-94-8References: Ducheyne, P., (1987) J. Biomed. Mater. Res.: Appl. Biomater., 21 (A2), p. 219;Hench, L.L., (1989) J. Biomed. Mater. Res., 23, p. 685; 
Hench, L.L., (1991) J. Amer. Ceram. Soc., 7, p. 1487; 
Jarcho, M., (1988) Bioceramics 1, p. 57. , edited by H. Oonishi, H. Aoki and K. Sawai Ishiyaku Euro America, Tokyo; 
De Groot, K., (1980) Biomaterials, 1, p. 47; 
Blitterswijk, C.A.V., Koerten, H.K., Grote, J.J., (1986) Biological and Biomechanical Performance of Biomaterials, p. 27. , edited by P. Cristel, A. Meunier and A. J. C. Lee Elsevier Science Publishers, Amsterdam; 
Ono, K., Yamamuro, T., Nakamura, T., Kokubo, T., Kotoura, Y., (1988) J. Biomed. Mater. Res., 22, p. 869; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , Japanese Association of Apatite Science (JAAS), Takayama Press System Centre Co., Inc; 
Manabe, A., Shigematsu, M., Kobayashi, S., (1987) High Tech Ceramics, p. 63. , edited by P. Vincenzini Elsevier Science Publishers, Amsterdam; 
Kitsugi, T., Yamamuro, T., Nakamura, T., Kokubo, T., Ono, M., (1987) J. Biomed. Mater. Res., 21, p. 1109; 
Kokubo, T., Ito, S., Shigematsu, M., Sakka, S., Yamamuro, T., (1985) J. Mater. Sci. Mater. Med., 20, p. 2001; 
Mestral, F., Drew, R.L.A., (1989) J. Eur. Ceram. Soc., 5, p. 47; 
Burnie, J., Gilchrist, T., Kobayashi, S., (1983) Ceramics in Surgery, p. 169. , edited by P. Vincenzini Elsevier Science Publishers, Amsterdam; 
Andersson, O.H., Liu, G., Karlsson, K.H., Niemi, L., Juhanoja, J., (1990) J. Mater. Sci. Mater. Med., 1, p. 219; 
Knowles, J.C., Rehman, I., Bonfield, W., (1994) Bioceramics 7, p. 85. , edited by O. H. Andersson, R. P. Happonen and A. Yli-Urpo Butterworth Heinemann, Turku, Finland; 
Santos, J.D., Reis, R.L., Knowles, J.C., Hastings, G.W., Monteiro, F.J., (1994) Biomaterials, 15, p. 5; 
Santos, J.D., Knowles, J.C., Monteiro, F.J., Hastings, G.W., (1992) Bioceramics 5, p. 35. , edited by T. Yamamuro, T. Kokubo and T. Nakamura Kokunshi Kankokai, Kyoto; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1985) J. Mater. Sci. Mater. Med., 6, p. 448; 
Wilson, J., Low, S., (1992) J. Appl. Biomater., 3, p. 123; 
Oonishi, H., Kushitani, S., Yasukawa, E., Hench, L.L., Wilson, J., Tsuji, E., Sugihara, T., (1994) Bioceramics 7, p. 139. , edited by O. H. Andersson, R.-P. Happonen and A. Yli-Urpo, Butterworth Heinemann, Turku, Finland; 
Neo, M., Nakamura, T., Yamamuro, T., Ohtsuki, C., Kokubo, T., Bando, Y., (1992) J. Biomed. Mater. Res., 26, p. 1419; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Kokubo, T., Kushitani, H., (1990) J. Biomed. Mater. Res., 24, p. 471; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., (1990) J. Biomed. Mater. Res., 24, p. 721; 
Santos, J.D., Jha, L.J., Monteiro, F.J., (1995) Biomaterials, 16, p. 521; 
Li, P., Ohtsuki, C., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., (1993) J. Appl. Biomater., 4, p. 221; 
Neo, M., Kotani, S., Fujita, Y., Yamamuro, T., Bando, Y., Ohtsuki, C., Kokubo, T., (1992) J. Biomed. Mater. Res., 26, p. 255; 
Li, P., Yang, Q., Zhang, F., Kokubo, T., (1992) J. Mater. Sci. Mater. Med., 3, p. 452; 
Kokubo, T., Kushitani, H., Kitsugi, T., Yamamuro, T., (1988) Bioceramics 1, p. 157. , edited by T. Yamamuro, T. Kokubo and T. Nakamura Kokunshi Kankokai, Kyoto; 
Landis, W.J., Martin, J.R., (1984) J. Vac. Sci. Technol., A2, p. 1108; 
Andersson, O.H., Kangasniemi, I., (1991) J. Biomed. Mater. Res., 25, p. 1019; 
Verhoeven, J.A.T., Van Doveren, H., (1982) Surf. Sci., 123, p. 369; 
Rehman, I., Hench, L.L., Bonfield, W., (1994) Bioceramics 7, p. 123. , edited by O. H. Andersson, R. P. Happonen and A. Yli-Urpo Butterworth Heinemann, Turku, Finland; 
Turner, N.H., Murday, J.S., Ramaker, D.E., (1980) Anal. Chem., 52, p. 84
},
	month = {1996///},
	pages = {181 - 185},
	publisher = {Chapman \& Hall Ltd},
	abstract = {In-vitro apatite layer formation on the surface of a newly developed glass reinforced hydroxyapatite composite was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDXA). After 1 week soaking in an acellular simulated body fluid the composite surface was entirely covered by a Ca, P-layer, suggesting a bioactive behaviour. XPS binding energy results revealed that this surface layer was a carbonated and non- stoichiometric apatite with Ca/P ratio of 1.3. This apatite layer was composed of very fine needle-like crystallites. Comparative studies on a commercially available hydroxyapatite showed that a similar apatite layer was also formed on its surface.In-vitro apatite layer formation on the surface of a newly developed glass reinforced hydroxyapatite composite was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDXA). After 1 week soaking in an acellular simulated body fluid the composite surface was entirely covered by a Ca, P-layer, suggesting a bioactive behaviour. XPS binding energy results revealed that this surface layer was a carbonated and non-stoichiometric apatite with Ca/P ratio of 1.3. This apatite layer was composed of very fine needle-like crystallites. Comparative studies on a commercially available hydroxyapatite showed that a similar apatite layer was also formed on its surface.
},
	keywords = {article, Binding energy, Biocompatibility, Biodegradation, biomaterial, Calcium, calcium phosphate, Characterization, Composite materials, Composition, Energy dispersive x ray analysis, glass, Glass reinforced hydroxyapetite composite, hydroxyapatite, implant, In vitro calcium phosphate formation, Ionic concentration, molecular interaction, Nucleation, Phosphates, phosphorus, priority journal, Scanning electron microscopy, Surfaces, X ray analysis, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030106254\&partnerID=40\&md5=725eba3e273e23b5c6519d07483495e8},
	author = {Santos, J.D. and Jha, L.J. and Monteiro, F.J.}
}
@article {2616,
	title = {Potentiometric Determination of Total and Ionized Calcium in Osteoblast-Like Cell Culture Medium},
	journal = {ElectroanalysisElectroanalysis},
	volume = {8},
	year = {1996},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: ELANELanguage of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Inst. de Engenharia Biom{\'e}dica, Faculdade de Engenharia, Universidade do Porto, Pra{\c c}a Coronel Pacheco, 1, P-4050 Porto, PortugalReferences: Nernst, W., (1886) Z. Phys. Chem., 2, p. 613;Nernst, W., (1889) Z. Phys. Chem., 4, p. 129; 
Morf, W.E., (1981) The Principle of Ion-Selective Electrodes and of Membrane Transport, , Elsevier, Amsterdam; 
Hulanicki, A., Trojanowicz, M., (1974) Anal. Chim. Acta, 68, p. 155; 
Anker, P., Wieland, E., Ammann, D., Dohner, R.E., Asper, R., Simon, W., (1981) Anal. Chem., 53, p. 1970; 
Manzoni, A., Premoli, P., (1985) Methodology and Clinical Applications of Ion-Selective Electrodes, pp. 131-136. , (Eds: A. H. J. Maas, A. B. T. J. Boink, N. E. L. Saris, R. Sprokholt, P. D. Wimberley), Radiometer, Copenhagen; 
Hulanicki, A., Lewandowski, R., Michalska, A., Lewenstam, A., (1990) Anal. Chim. Acta, 233, p. 269; 
Morais, S., Sousa, J.P., Fernandes, H., Carvalho, G.S., (1994) 11th European Conference on Biomaterials, Pisa, pp. 10-11. , Last Minute Posters Abstracts Book, European Society for Biomaterials; 
Ruzicka, J., Tjell, J.C., (1969) Anal. Chim. Acta, 47, p. 475; 
Ross, J.W., (1967) Science, 15, p. 1378; 
Rechnitz, G.A., Hseu, T.M., (1969) Anal. Chem., 41, p. 111; 
Oreskez, I., Hirsch, C., Douglas, K.S., Kupfer, S., (1968) Clin. Chim. Acta, 21, p. 203; 
Recomendations for Nomenclature of Ion-Selective Electrodes (1994) Pure Appl. Chem., 66, p. 2527; 
Ringbom, A., (1963) Complexation in Analytical Chemistry, , Interscience, New York; 
Russell Instruction Manual of Calcium Electrode Model 93-3209; 
Baudet, S., (1994) Methods in Cell Biology, pp. 93-113. , Academic Press, New York; 
Scharff, O., (1979) Anal. Chim. Acta, 109, p. 291
},
	month = {1996///},
	pages = {1174 - 1178},
	abstract = {The effect of the ionic strength and composition of solutions in the direct potentiometric determination of total and ionized calcium content using a calcium selective electrode was investigated. In the presence of a constant complexation buffer (CCB), the addition of sodium chloride and sodium bicarbonate causes a decrease in the limit of detection of the potentiometric response. When the procedure was tested in osteoblast-like cell culture medium no electrode interferences or method interferences were observed by the presence of proteins, i.e., the total and ionized calcium in this medium can be measured by potentiometric means.},
	keywords = {Cell culture medium, potentiometry, Total and ionized calcium},
	isbn = {10400397 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0000007614\&partnerID=40\&md5=e2532b414ac34fdd4bb8b71ac5cbabcd},
	author = {Morais, S. and Carvalho, G.S. and Sousa, J.P.}
}
@article {2624,
	title = {Reinforcement of hydroxyapatite by adding P                    2O
                    5-CaO glasses with Na
                    2O, K
                    2O and MgO
},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {7},
	year = {1996},
	note = {Cited By (since 1996): 49Export Date: 21 November 2012Source: ScopusCODEN: JSMMELanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; Dept. of Metallurgical Engineering, FEUP, University of Porto, Rua dos Bragas, 4099 Porto Codex, PortugalChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; magnesium oxide, 1309-48-4, 1317-74-4References: Barney, V.C., Levin, M.P., Adams, D.F., (1986) J. Perio Dont., 57, p. 764;Legeros, R.Z., (1988) Adv. Dent. Res., 2, p. 164; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, p. 165. , Takayama Press System Center, JAAS, Tokyo; 
Ducheyne, P., (1987) J. Biomed. Mater. Res. Appl. Biomater., 21, p. 219; 
Heughbaert, J.C., Bonel, G., (1986) Biological and Biomechanical Performance of Biomaterials, p. 9. , edited by P. Christel, A. Meunier and A. J. C. Lee Elsevier Science Publishers B.V., Amsterdam; 
Ellis, L.G., Nelson, D.G.A., Featherstone, J.D.B., (1988) J. Biomed. Mater. Res., 22, p. 541; 
Raemdonck, W.V., Ducheyne, P., Meester, P.D., (1984) Metal and Ceramic Biomaterials, p. 143. , edited by P. Ducheyne and G. W. Hashi CRC Press, Boca Raton, FL; 
Shors, E.C., Holmes, R.E., (1993) An Introduction to Bioceramics, p. 181. , edited by L. L. Hench and J. Wilson World Scientific, Hong Kong; 
Rey, C., (1990) Biomaterials, 11, p. 14; 
Evans, G., Behiri, J., Currey, J., Bonfield, W., (1990) J. Mater. Sci. Mater. Med., 1, p. 38; 
Rey, C., Fr{\`e}che, M., Heughebaert, M., Heughebaert, J.C., Lacout, J.L., Vignoles, M., Bioceramics 4, London, 1991, p. 57. , edited by W. Bonfield, G. W. Hastings and K. E. Tanner (Butterworth-Heinemann, London); 
With, G., Van Dijk, H., Hattu, N., Prijs, K., (1981) J. Mater. Sci., 16, p. 1592; 
Akao, M., Aoki, H., Kato, K., (1981) J. Mater. Sci., 16, p. 809; 
Santos, J., Knowles, J., Reis, R., Monteiro, F., Hastings, G., (1994) Biomaterials, 15, p. 5; 
Soltesz, U., Richter, H., Kienzler, R., (1987) High Technology Ceramics, p. 110. , Elsevier Science Publishers, B.V., Amsterdam; 
(1971) Determination Micrographique de la Grosseur du Grain Ferritique ou Aust{\'e}nitique des Aciers; 
Daculsi, G., LeGeros, R.G., Nery, E., Lynch, K., Kerebel, B., (1989) J. Biomed. Mater. Res., 23, p. 883
},
	month = {1996///},
	pages = {187 - 189},
	publisher = {Chapman \& Hall Ltd},
	abstract = {Commercial hydroxyapatite was reinforced by adding small amounts (2 and 4 wt\%) of P                        2O
                        5-based glasses during its sintering process. The composites prepared had a chemical composition closely related to the mineral part of bone tissues in terms of trace elements usually detected, such as Na, K and Mg. X-ray diffraction analysis (XRD) showed that the glass reinforced-HA composites were composed of a HA matrix and variable amounts of tricalcium phosphate phase, depending on sintering temperature and glass composition. These composites were shown to have much higher biaxial bending strength than sintered HA, 107 MPa for Ha/2\% of 35P
                        2O
                        5-35CAO-10Na
                        2O-10K
                        2O-10MgO glass composite and 28 MPa for sintered HA. The presence of β-tricalcium phosphate in the microstructure of the composites is an important factor in the reinforcement process.
Commercial hydroxyapatite was reinforced by adding small amounts (2 and 4 wt\%) of P
                        2O
                        5-based glasses during its sintering process. The composites prepared had a chemical composition closely related to the mineral part of bone tissues in terms of trace elements usually detected, such as Na, K and Mg. X-ray diffraction analysis (XRD) showed that the glass reinforced-HA composites were composed of a HA matrix and variable amounts of tricalcium phosphate phase, depending on sintering temperature and glass composition. These composites were shown to have much higher biaxial bending strength than sintered HA, 107 MPa for Ha/2\% of 35P
                        2O
                        5-35CaO-10Na
                        2O-10K
                        2O-10MgO glass composite and 28 MPa for sintered HA. The presence of β-tricalcium phosphate in the microstructure of the composites is an important factor in the reinforcement process.
},
	keywords = {article, Bending strength, Bone, chemical composition, Composite materials, Composition, Composition effects, glass, Glass composition, Glass reinforced hydroxapatite composite, hydroxyapatite, implant, Magnesium Oxide, metal oxide, Microstructure, molecular dynamics, Phosphates, potassium derivative, priority journal, Sintering, Sintering temperature, sodium derivative, Thermal effects, Trace Elements, Tricalcium phosphate phase, X ray diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030108677\&partnerID=40\&md5=e9c978c3e018a1dd2db9b534ef00ea1b},
	author = {Santos, J.D. and Silva, P.L. and Knowles, J.C. and Talal, S. and Monteiro, F.J.}
}
@article {2637,
	title = {Computerized fetal heart rate analysis in labour based on 2-s sampling. Can it proceed with confidence? [1]},
	journal = {European Journal of Obstetrics Gynecology and Reproductive BiologyEUR. J. OBSTET. GYNECOL. REPROD. BIOL.},
	volume = {63},
	year = {1995},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: EOGRAdoi: 10.1016/0301-2115(95)02215-SPubMed ID: 8674558Language of Original Document: EnglishCorrespondence Address: Bernardes, J.; Instituto Engenharia Biomedica, Praca Coronel Pacheco 1, 4000 Porto, Portugal},
	month = {1995///},
	pages = {105 - 107},
	keywords = {computer analysis, Computers, Female, Fetal monitoring, fetus, fetus heart rate, Heart Rate, Fetal, human, labor, Labor, Obstetric, letter, measurement, Pregnancy, priority journal, sampling, Time Factors},
	isbn = {03012115 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028856781\&partnerID=40\&md5=d0c302fff078192103c46bde7d37746f},
	author = {Bernardes, J. and Garrido, A. and Chang, A. and Sahota, D.S. and Reed, N.N. and James, D.K. and Mohajer, M.P.}
}
@article {2634,
	title = {Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions: Structure and properties of the composites},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {6},
	year = {1995},
	note = {Cited By (since 1996): 41Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00120303Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB-National Inst. Biomedical Engn., Department Metallurgical Engineering, FEUP, University of Porto, Rua dos Bragas 4099-Porto Codex, Portugal},
	month = {1995///},
	pages = {348 - 352},
	publisher = {Chapman \& Hall Ltd},
	abstract = {Phosphate- and silicate-based glasses were added to hydroxyapatite in order to improve its mechanical properties and to fabricate composites with different degrees of bioactivity. A strong chemical bonding was obtained between hydroxyapatite and the phosphate-based glasses leading to samples approaching theoretical density, according to density measurements and scanning electron microscopy. Bioglass{\textregistered} additions led to the formation of a complex calcium phosphate silicate which hampered the reinforcement process. The fracture toughness of the hydroxyapatite-glass composites was shown to be within the 1.1-1.2 MPam(1/2) range, which is double that determined for sintered hydroxyapatite. A 2 μm thick apatite layer was observed on the surface of the hydroxyapatite-glass composites after 48 h immersion in a simulated human blood plasma, whereas only a few apatite crystals were detected on sintered hydroxyapatite after 7 days immersion. From the results obtained we anticipate that the composites might show a higher rate of bone bonding, leading to enhanced bioactivity.Phosphate- and silicate-based glasses were added to hydroxyapatite in order to improve its mechanical properties and to fabricate composites with different degrees of bioactivity. A strong chemical bonding was obtained between hydroxyapatite and the phosphate-based glasses leading to samples approaching theoretical density, according to density measurements and scanning electron microscopy. Bioglass
                        (R) additions led to the formation of a complex calcium phosphate silicate which hampered the reinforcement process. The fracture toughness of the hydroxyapatite-glass composites was shown to be within the 1.1-1.2 MPa m
                        1/2 range, which is double that determined for sintered hydroxyapatite. A 2 μm thick apatite layer was observed on the surface of the hydroxyapatite-glass composites after 48 h immersion in a simulated human blood plasma, whereas only a few apatite crystals were detected on sintered hydroxyapatite after 7 days immersion. From the results obtained we anticipate that the composites might show a higher rate of bone bonding, leading to enhanced bioactivity.
},
	keywords = {Adhesion, article, Bioactivity, Bioglass, Biomaterials, Bone, bone prosthesis, calcium phosphate, chemical binding, Chemical bonds, chemical composition, Chemical structure, Complex calcium phosphate silicate, Composite materials, crystal, density, Density measurement (specific gravity), glass, Human blood plasma, hydroxyapatite, Liquid phase sintering, Mechanical properties, Mechanics, phosphate, Phosphates, plasma, priority journal, Scanning electron microscopy, silicate, Silicates, Sintering, Structure (composition), surface property, thickness},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029311049\&partnerID=40\&md5=433498e286bfad74a296d1fafc50c223},
	author = {Santos, J.D. and Reis, R.L. and Monteiro, F.J. and Knowles, J.C. and Hastings, G.W.}
}
@article {2629,
	title = {Modifications in the molecular structure of hydroxyapatite induced by titanium ions},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {6},
	year = {1995},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00134326Language of Original Document: EnglishCorrespondence Address: Ribeiro, C.C.; Instituto de Engenharia Biomedica, Praca do Coronel Pacheco, 1, 4050 Porto, Portugal},
	month = {1995///},
	pages = {829 - 834},
	publisher = {Chapman \& Hall Ltd},
	abstract = {The purpose of this study was to contribute to the understanding of the effect of titanium ions released from a metallic substrate on the molecular structure of hydroxyapatite. The effects of pH, time of incubation and concentration of titanium ions were investigated. The solids were analysed by X-ray diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, energy dispersive X-ray analysis and thermogravimetric analysis. The results clearly indicate the presence of a titanium phosphate, Ti(HPO                        4)
                        2{\textperiodcentered}nH
                        2O (n = 1-3), which probably has a double layered structure. The formation of this compound is dependent on the titanium concentration and its crystallinity increases with the time of incubation.
The purpose of this study was to contribute to the understanding of the effect of titanium ions released from a metallic substrate on the molecular structure of hydroxyapatite. The effects of pH, time of incubation and concentration of titanium ions were investigated. The solids were analysed by X-ray diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, energy dispersive X-ray analysis and thermogravimetric analysis. The results clearly indicate the presence of a titanium phosphate, Ti(HPO
                        4)
                        2{\textperiodcentered}nH
                        2O (n = 1-3), which probably has a double layered structure. The formation of this compound is dependent on the titanium concentration and its crystallinity increases with the time of incubation.
},
	keywords = {Calcium Compounds, Chemical structure, Coatings, conference paper, Crystallinity, Crystallization, Dissolution, Energy dispersive x ray analysis, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, hydroxyapatite, Implants (surgical), incubation time, infrared spectroscopy, Ions, metal ion, Molecular Structure, pH, pH effects, phosphate, Phosphates, priority journal, Raman spectrometry, Raman spectroscopy, Thermogravimetric analysis, thermogravimetry, time, Titanium, Titanium compounds, X ray analysis, X ray diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029583899\&partnerID=40\&md5=724de7ed1c6fddd7a7e258cd19ba3710},
	author = {Ribeiro, C.C. and Barbosa, M.A. and Machado, A.A.S.C. and Tudor, A. and Davies, M.C.}
}
@article {2633,
	title = {Plasma surface treatment and PACVD on Ti alloys for surgical implants},
	journal = {Surface and Coatings TechnologySurf. Coat. Technol.},
	volume = {74-75},
	year = {1995},
	note = {Cited By (since 1996): 49Export Date: 21 November 2012Source: Scopusdoi: 10.1016/0257-8972(95)08316-2Language of Original Document: EnglishCorrespondence Address: Rie, K.-T.; Institut f{\"u}r Oberfl{\"a}chentechnik und plasmatechnische Werkstoffentwicklung, Technische Universit{\"a}t Braunschweig, Bienroder Weg 53, D-38108 Braunschweig, Germany},
	month = {1995///},
	pages = {973 - 980},
	abstract = {To improve the poor surface hardness and wear resistance of titanium alloys used for surgical implants undergoing wear TiA16V4 and TiA15Fe2.5 have been plasma nitrided. TiN and other sublayers are formed by plasma nitriding. To increase the thickness of the outermost TiN layer the samples have been coated with TiN by PACVD following plasma nitriding. The produced layers have been characterized in terms of microstructure, layer composition, hardness depth profiles. The evaluation of corrosion resistance and biocompatibility revealed that the good properties of the untreated alloys have been well preserved. {\textcopyright} 1995 Elsevier Science S.A.},
	keywords = {Biocompatibility, Biomaterials, Characterization, Chemical vapor deposition, Corrosion resistance, hardness, Implants (surgical), Microstructure, Nitriding, PACVD, Plasma applications, Plasma assisted chemical vapor deposition, Plasma nitriding, Plasma surface treatment, Surface hardness, Surface treatment, Surfaces, Ti alloys, Titanium alloys, Wear resistance},
	isbn = {02578972 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029389521\&partnerID=40\&md5=289d2d683bcdc5c62d1ecdf56b33079a},
	author = {Rie, K.-T. and Stucky, T. and Silva, R.A. and Leitao, E. and Bordji, K. and Jouzeau, J.-Y. and Mainard, D.}
}
@article {2635,
	title = {Surface modifications of glass-reinforced hydroxyapatite composites},
	journal = {BiomaterialsBIOMATERIALS},
	volume = {16},
	year = {1995},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/0142-9612(95)91124-HPubMed ID: 7492715Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Dept Metallurgical Engineering, FEUP, University of Porto, Rua dos Bragas 4099-Porto Codex, PortugalChemicals/CAS: Biocompatible Materials; Calcium, 7440-70-2; Glass; Hydroxyapatites; Phosphorus, 7723-14-0; Silicon, 7440-21-3; Sodium, 7440-23-5},
	month = {1995///},
	pages = {521 - 526},
	publisher = {Butterworth-Heinemann Ltd},
	abstract = {Surface modifications of glass-reinforced hydroxyapatite composites immersed in a simulated physiological solution were studied using X-ray photoelectron spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy. In the first stages of the apatite formation process, calcium and silicon ions were leached out from the surface of the composites. After 12 days of immersion, apatite crystals were detected on the surface indicating bioactive behaviour. Sodium ions attributed to sodium sulphate were also found in the apatite layer. Bound water on the surface also increased with immersion time.Surface modifications of glass-reinforced hydroxyapatite composites immersed in a simulated physiological solution were studied using X-ray photoelectron spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy. In the first stages of the apatite formation process, calcium and silicon ions were leached out from the surface of the composites. After 12 days of immersion, apatite crystals were detected on the surface indicating bioactive behaviour. Sodium ions attributed to sodium sulphate were also found in the apatite layer. Bound water on the surface also increased with immersion time.
},
	keywords = {Apatite, article, Bioactive glass, Biocompatible Materials, Biomaterials, Calcium, chemical composition, Composite materials, Electron Probe Microanalysis, glass, Glass reinforced hydroxyapatite composites, Hydrogen-Ion Concentration, hydroxyapatite, Hydroxyapatites, immersion, Ions, Leaching, Microscopy, Electron, Scanning, Phosphates, phosphorus, priority journal, Scanning electron microscopy, Silicon, Sodium, sodium chloride, sodium sulfate, Spectrometry, X-Ray Emission, Spectroscopy, Surface modification, Surface Properties, surface property, Surface treatment, Water, X ray, X ray photoelectron spectroscopy, X ray spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029294502\&partnerID=40\&md5=2694e1944afd0945ac391ba41c4ec7b5},
	author = {Santos, J.D. and Jha, L.J. and Monteiro, F.J.}
}
@article {2639,
	title = {Stability of hydroxylapatite plasma-sprayed coated Ti-6Al-4V under cyclic bending in simulated physiological solutions},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {5},
	year = {1994},
	note = {Cited By (since 1996): 35Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00058983Language of Original Document: EnglishCorrespondence Address: Reis, R.L.; INEB-National Inst. Biomedical Engn., Faculty of Engineering, University of Porto, Rua dos Bragas, 4099 Porto Codex, Portugal},
	month = {1994///},
	pages = {457 - 462},
	publisher = {Chapman \& Hall Ltd},
	abstract = {The aim of this research was to study the stability of plasma-sprayed coated metal systems and to evaluate their susceptibility to the occurrence of corrosion fatigue. Hydroxylapatite plasma-sprayed coated samples of Ti-6Al-4V were studied under cyclic bending. During fatigue testing samples were immersed in a simulated physiological solution and mechanical and electrochemical degradation were monitored. Applied loads were intended to crack the ceramic coating and not the metal substrate. Electrochemical impedance spectroscopy was used to further characterize the electrochemical behaviour. No increase in tendency to corrode was detected in open-circuit corrosion fatigue testing. It appears as if the coating cracking does not increase metal substrate corrosion susceptibility. The coating integrity has been seriously affected, with marked decrease in thickness, due to the synergistic effect of load and presence of simulated body fluids environment. Impedance results, however, show a general tendency to an increase in corrosion kinetics after corrosion fatigue testing.The aim of this research was to study the stability of plasma-sprayed coated metal systems and to evaluate their susceptibility to the occurrence of corrosion fatigue. Hydroxylapatite plasma-sprayed coated samples of Ti-6Al-4V were studied under cyclic bending. During fatigue testing samples were immersed in a simulated physiological solution and mechanical and electrochemical degradation were monitored. Applied loads were intended to crack the ceramic coating and not the metal substrate. Electrochemical impedance spectroscopy was used to further characterize the electrochemical behaviour. No increase in tendency to corrode was detected in open-circuit corrosion fatigue testing. It appears as if the coating cracking does not increase metal substrate corrosion susceptibility. The coating integrity has been seriously affected, with marked decrease in thickness, due to the synergistic effect of load and presence of simulated body fluids environment. Impedance results, however, show a general tendency to an increase in corrosion kinetics after corrosion fatigue testing.
},
	keywords = {article, Bending (deformation), biomaterial, biomechanics, body fluid, Ceramic coatings, ceramics, controlled study, Corrosion, Corrosion fatigue, Corrosion susceptibility, Degradation, Electrochemical impedance spectroscopy, electrochemistry, fatigue, Fatigue testing, hydroxyapatite, impedance, mechanical stress, Physiological solutions, Plasma spraying, priority journal, sodium chloride, Solutions, Spectroscopy, Stability, Substrates, thickness, Titanium, Titanium alloys},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028447410\&partnerID=40\&md5=f863751be3003b31534dd128601835f8},
	author = {Reis, R.L. and Monteiro, F.J. and Hastings, G.W.}
}
