



@article {3391,
	title = {Coffee silverskin: A possible valuable cosmetic ingredient},
	journal = {Pharmaceutical Biology},
	volume = {53},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {386-394},
	isbn = {1388-0209},
	url = {<Go to ISI>://WOS:000348831200011},
	author = {Rodrigues, Francisca and Palmeira-de-Oliveira, Ana and das Neves, Jose and Sarmento, Bruno and Helena Amaral, M. and Oliveira, M. Beatriz P. P.}
}
@article {3387,
	title = {Finite element analysis of pectus carinatum surgical correction via a minimally invasive approach},
	journal = {Computer Methods in Biomechanics and Biomedical Engineering},
	volume = {18},
	year = {2015},
	note = {Times Cited: 0Neves, Sara/A-5993-2015
Neves, Sara/0000-0001-6154-2821
},
	month = {2015},
	pages = {711-720},
	isbn = {1025-5842},
	url = {<Go to ISI>://WOS:000345141700003},
	author = {Neves, Sara C. and Pinho, A. C. M. and Fonseca, Jaime C. and Rodrigues, Nuno F. and Henriques-Coelho, Tiago and Correia-Pinto, Jorge and Vilaca, Joao L.}
}
@article {3232,
	title = {Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated PLGA nanoparticles for theranostic application},
	journal = {International Journal of Nanomedicine},
	volume = {9},
	year = {2014},
	month = {2014},
	pages = {4911-4922},
	url = {<Go to ISI>://WOS:000343406300001},
	author = {Moura, Catarina Costa and Segundo, Marcela A. and das Neves, Jose and Reis, Salette and Sarmento, Bruno}
}
@article {3409,
	title = {Differences between external and internal fetal heart rate monitoring during the second stage of labor: a prospective observational study},
	journal = {Journal of Perinatal Medicine},
	volume = {42},
	year = {2014},
	month = {2014},
	pages = {493-498},
	url = {<Go to ISI>://WOS:000338934800012},
	author = {Nunes, Ines and Ayres-de-Campos, Diogo and Costa-Santos, Cristina and Bernardes, Joao}
}
@article {3406,
	title = {Endoplasmic Reticulum Degradation-Enhancing alpha-Mannosidase-like Protein 1 Targets Misfolded HLA-B27 Dimers for Endoplasmic Reticulum-Associated Degradation},
	journal = {Arthritis \& Rheumatology},
	volume = {66},
	year = {2014},
	month = {2014},
	pages = {2976-2988},
	url = {<Go to ISI>://WOS:000344349500008},
	author = {Guiliano, David B. and Fussell, Helen and Lenart, Izabela and Tsao, Edward and Nesbeth, Darren and Fletcher, Adam J. and Campbell, Elaine C. and Yousaf, Nasim and Williams, Sarah and Santos, Susana and Cameron, Amy and Towers, Greg J. and Kellam, Paul and Hebert, Daniel N. and Gould, Keith G. and Powis, Simon J. and Antoniou, Antony N.}
}
@article {3294,
	title = {Eradication of Helicobacter pylori: Past, present and future},
	journal = {Journal of Controlled Release},
	volume = {189},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {169 - 186},
	abstract = {Helicobacter pylori is the major cause of chronic gastritis and peptic ulcers. Since the classification as a group 1 carcinogenic by International Agency for Research on Cancer, the importance of the complete H. pylori eradication has obtained a novel meaning. Hence, several studies have been made in order to deepen the knowledge in therapy strategies. However, the current therapy presents unsatisfactory eradication rates due to the lack of therapeutic compliance, antibiotic resistance, the degradation of antibiotics at gastric pH and their insufficient residence time in the stomach. Novel approaches have been made in order to overcome these limitations. The purpose of this review is to provide an overview about the current therapy and its limitations, while highlighting the possibility of using micro- and nanotechnology to develop gastric drug delivery systems, overcoming these difficulties in the future. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {Drug delivery, Helicobacter pylori, Microparticles, nanoparticles, Treatment},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904904149\&partnerID=40\&md5=96cfe2d7f96a7c7a16bb7eb733c2b71c},
	author = {Lopes, D. and Nunes, C. and Martins, M.C.L. and Sarmento, B. and Reis, S.}
}
@article {3410,
	title = {Formulation and delivery of anti-HIV rectal microbicides: Advances and challenges},
	journal = {Journal of Controlled Release},
	volume = {194},
	year = {2014},
	month = {2014},
	pages = {278-294},
	url = {<Go to ISI>://WOS:000344229800027},
	author = {Nunes, Rute and Sarmento, Bruno and das Neves, Jose}
}
@article {3303,
	title = {Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure},
	journal = {ACS Nano},
	volume = {8},
	year = {2014},
	note = {Cited By (since 1996):4Export Date: 21 November 2014},
	month = {2014},
	pages = {2033 - 2047},
	abstract = {Stem cell responsiveness to extracellular matrix (ECM) composition and mechanical cues has been the subject of a number of investigations so far, yet the molecular mechanisms underlying stem cell mechano-biology still need full clarification. Here we demonstrate that the paralog proteins YAP and TAZ exert a crucial role in adult cardiac progenitor cell mechano-sensing and fate decision. Cardiac progenitors respond to dynamic modifications in substrate rigidity and nanopattern by promptly changing YAP/TAZ intracellular localization. We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design. {\textcopyright} 2014 American Chemical Society.},
	keywords = {adult cardiac progenitor cell, cardiac differentiation, mechano- transduction, substrate nanotopography, YAP/TAZ},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896911335\&partnerID=40\&md5=c15ea687a167227f6d91e71dcc812590},
	author = {Mosqueira, D. and Pagliari, S. and Uto, K and Ebara, M and Romanazzo, S. and Escobedo-Lucea, C and Nakanishi, J and Taniguchi, A and Franzese, O and Di Nardo, P. and Goumans, MJ and Traversa, E and Pinto-Do-{\'O}, P. and Aoyagi, T. and Forte, G.}
}
@article {3305,
	title = {Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms},
	journal = {Stem Cell Research and Therapy},
	volume = {5},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	abstract = {Introduction. Among the plethora of cells under investigation to restore a functional myocardium, mesenchymal stromal cells (MSCs) have been granted considerable interest. However, whereas the beneficial effects of bone marrow MSCs (BM-MSCs) in the context of the diseased heart are widely reported, data are still scarce on MSCs from the umbilical cord matrix (UCM-MSCs). Herein we report on the effect of UCM-MSC transplantation to the infarcted murine heart, seconded by the dissection of the molecular mechanisms at play. Methods. Human umbilical cord tissue-derived MSCs (UCX{\textregistered}), obtained by using a proprietary technology developed by ECBio, were delivered via intramyocardial injection to C57BL/6 females subjected to permanent ligation of the left descending coronary artery. Moreover, medium produced by cultured UCX{\textregistered} preconditioned under normoxia (CM) or hypoxia (CMH) was collected for subsequent in vitro assays. Results: Evaluation of the effects upon intramyocardial transplantation shows that UCX{\textregistered} preserved cardiac function and attenuated cardiac remodeling subsequent to myocardial infarction (MI). UCX{\textregistered} further led to increased capillary density and decreased apoptosis in the injured tissue. In vitro, UCX{\textregistered}-conditioned medium displayed (a) proangiogenic activity by promoting the formation of capillary-like structures by human umbilical vein endothelial cells (HUVECs), and (b) antiapoptotic activity in HL-1 cardiomyocytes subjected to hypoxia. Moreover, in adult murine cardiac Sca- 1{\textsection}ssup{\textsection}+{\textsection}esup{\textsection} progenitor cells (CPCs), conditioned medium enhanced mitogenic activity while activating a gene program characteristic of cardiomyogenic differentiation. Conclusions: UCX{\textregistered} preserve cardiac function after intramyocardial transplantation in a MI murine model. The cardioprotective effects of UCX{\textregistered} were attributed to paracrine mechanisms that appear to enhance angiogenesis, limit the extent of the apoptosis, augment proliferation, and activate a pool of resident CPCs. Overall, these results suggest that UCX{\textregistered} should be considered an alternative cell source when designing new therapeutic approaches to treat MI. {\textcopyright} 2014 Nascimento et al.; licensee BioMed Central Ltd.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896713037\&partnerID=40\&md5=22fdca81d834ec78c8196552c3d250ae},
	author = {Nascimento, D.S. and Mosqueira, D. and Sousa, L.M. and Teixeira, M. and Filipe, M. and Resende, TP and Ara{\'u}jo, A.F. and Valente, M. and Almeida, J. and Martins, J.P. and Santos, J.M. and B{\'a}rcia, R.N. and Cruz, P. and Cruz, H. and Pinto-Do-{\'O}, P.}
}
@booklet {3260,
	title = {Impact of 3-D printed PLA- and chitosan-based scaffolds on human monocyte/macrophage responses: Unraveling the effect of 3-D structures on inflammation},
	howpublished = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {613 - 622},
	abstract = {Recent studies have pointed towards a decisive role of inflammation in triggering tissue repair and regeneration, while at the same time it is accepted that an exacerbated inflammatory response may lead to rejection of an implant. Within this context, understanding and having the capacity to regulate the inflammatory response elicited by 3-D scaffolds aimed for tissue regeneration is crucial. This work reports on the analysis of the cytokine profile of human monocytes/macrophages in contact with biodegradable 3-D scaffolds with different surface properties, architecture and controlled pore geometry, fabricated by 3-D printing technology. Fabrication processes were optimized to create four different 3-D platforms based on polylactic acid (PLA), PLA/calcium phosphate glass or chitosan. Cytokine secretion and cell morphology of human peripheral blood monocytes allowed to differentiate on the different matrices were analyzed. While all scaffolds supported monocyte/macrophage adhesion and stimulated cytokine production, striking differences between PLA-based and chitosan scaffolds were found, with chitosan eliciting increased secretion of tumor necrosis factor (TNF)-α, while PLA-based scaffolds induced higher production of interleukin (IL)-6, IL-12/23 and IL-10. Even though the material itself induced the biggest differences, the scaffold geometry also impacted on TNF-α and IL-12/23 production, with chitosan scaffolds having larger pores and wider angles leading to a higher secretion of these pro-inflammatory cytokines. These findings strengthen the appropriateness of these 3-D platforms to study modulation of macrophage responses by specific parameters (chemistry, topography, scaffold architecture). {\textcopyright} 2013 Acta Materialia Inc.},
	keywords = {3-D scaffolds, Human macrophages, Inflammation, Rapid prototyping, Tissue engineering},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896505907\&partnerID=40\&md5=948f87b684be751bc6eb444e28ce6025},
	author = {Almeida, C.R. and Serra, T. and Oliveira, M.I. and Planell, J.A. and Barbosa, M.A. and Navarro, M.}
}
@article {3265,
	title = {Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation},
	journal = {Journal of Biomaterials Applications},
	volume = {28},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1325 - 1335},
	abstract = {Nanohydroxyapatite (nanoHA), due to its chemical properties, has appeared as an exceptionally promising bioceramic to be used as bone regeneration material. Staphylococcus epidermidis have emerged as major nosocomial pathogens associated with infections of implanted medical devices. In this work, the purpose was to study the influence of the nanoHA surface characteristics on S. epidermidis RP62A biofilm formation. Therefore, two different initial inoculum concentrations (Ci) were used in order to check if these would affect the biofilm formed on the nanoHA surfaces. Biofilm formation was followed by the enumeration of cultivable cells and by scanning electron microscopy. Surface topography, contact angle, total surface area and porosimetry of the biomaterials were studied and correlated with the biofilm data. The surface of nanoHA sintered at 830 (nanoHA830) showed to be more resistant to S. epidermidis attachment and accumulation than that of nanoHA sintered at 1000 (nanoHA1000). The biofilm formed on nanoHA830 presented differences in terms of structure, surface coverage and EPS production when compared to the one formed on nanoHA1000 surface. It was observed that topography and surface area of nanoHA surfaces had influence on the bacterial attachment and accumulation. Ci influenced bacteria attachment and accumulation on nanoHA surfaces over time. The choice of the initial inoculum concentration was relevant proving to have an effect on the extent of adherence thus being a critical point for human health if these materials are used in implantable devices. This study showed that the initial inoculum concentration and surface material properties determine the rate of microbial attachment to substrata and consequently are related to biofilm-associated infections in biomaterials. {\textcopyright} The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.},
	keywords = {biofilm formation, biomaterial roughness, biomaterial surface area, Inoculum concentration, Nanohydroxyapatite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84890640286\&partnerID=40\&md5=0f7d5eb37db34b3d0a1e4fb334846340},
	author = {Barros, J. and Grenho, L. and Manuel, C.M. and Ferreira, C. and Melo, L. and Nunes, O.C. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {3304,
	title = {Mad2 checkpoint gene silencing using epidermal growth factor receptor-targeted chitosan nanoparticles in non-small cell lung cancer model},
	journal = {Molecular Pharmaceutics},
	volume = {11},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {3515 - 3527},
	abstract = {RNA interference has emerged as a powerful strategy in cancer therapy because it allows silencing of specific genes associated with tumor progression and resistance. Mad2 is an essential mitotic checkpoint component required for accurate chromosome segregation during mitosis, and its complete abolition leads to cell death. We have developed an epidermal growth factor receptor (EGFR)-targeted chitosan system for silencing the Mad2 gene as a strategy to efficiently induce cell death in EGFR overexpressing human A549 non-small cell lung cancer cells. Control and EGFR-targeted chitosan nanoparticles loaded with small interfering RNAs (siRNAs) against Mad2 were formulated and characterized for size, charge, morphology, and encapsulation efficiency. Qualitative and quantitative intracellular uptake studies by confocal imaging and flow cytometry, respectively, showed time-dependent enhanced and selective intracellular internalization of EGFR-targeted nanoparticles compared to nontargeted system. Targeted nanoparticles showed nearly complete depletion of Mad2 expression in A549 cells contrasting with the partial depletion in the nontargeted system. Accordingly, Mad2-silencing-induced apoptotic cell death was confirmed by cytotoxicity assay and flow cytometry. Our results demonstrate that EGFR-targeted chitosan loaded with Mad2 siRNAs is a potent delivery system for selective killing of cancer cells.},
	keywords = {chitosan nanoparticles, Epidermal growth factor receptor, Mad2 gene, non-small cell lung cancer, RNA interference, tumor targeting},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84908009342\&partnerID=40\&md5=bc0130ca0bcdb576b7de6801c3517e93},
	author = {Nascimento, A.V. and Singh, A. and Bousbaa, H. and Ferreira, D. and Sarmento, B. and Amiji, M.M.}
}
@article {3413,
	title = {Microfluidics co-culture systems for studying tooth innervation},
	journal = {Frontiers in Physiology},
	volume = {5},
	year = {2014},
	note = {Times Cited: 0Lamghari, Meriem/K-2159-2013
Lamghari, Meriem/0000-0002-2500-4548
},
	month = {2014},
	isbn = {1664-042X},
	url = {<Go to ISI>://WOS:000347083900001},
	author = {Pagella, Pierfrancesco and Neto, Estrela and Jimenez-Rojo, Lucia and Lamghari, Meriem and Mitsiadis, Thimios A.}
}
@article {3421,
	title = {Mucoadhesive polymers in the design of nano-drug delivery systems for administration by non-parenteral routes: A review},
	journal = {Progress in Polymer Science},
	volume = {39},
	year = {2014},
	note = {Times Cited: 1},
	month = {2014},
	pages = {2030-2075},
	isbn = {0079-6700},
	url = {<Go to ISI>://WOS:000347134000004},
	author = {Sosnik, Alejandro and das Neves, Jose and Sarmento, Bruno}
}
@article {3408,
	title = {Prolonged saltatory fetal heart rate pattern leading to newborn metabolic acidosis},
	journal = {Clinical and Experimental Obstetrics \& Gynecology},
	volume = {41},
	year = {2014},
	month = {2014},
	pages = {507-511},
	url = {<Go to ISI>://WOS:000343961800003},
	author = {Nunes, I. and Ayres-de-Campos, D. and Kwee, A. and Rosen, K. G.}
}
@article {3326,
	title = {Sca-1+cardiac progenitor cells and heart-making: A critical synopsis},
	journal = {Stem Cells and Development},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2263 - 2273},
	abstract = {The identification, in the adult, of cardiomyocyte turnover events and of cardiac progenitor cells (CPCs) has revolutionized the field of cardiovascular medicine. However, the low rate of CPCs differentiation events reported both in vitro and in vivo, even after injury, raised concerns on the biological significance of these subsets. In this Comprehensive Review, we discuss the current understanding of cardiac Lin-Sca-1+cells in light of what is also known for cellular compartments with similar phenotypes in other organs. The Lin-Sca-1+heart subset is heterogeneous and displays a mesenchymal profile, characterized by a limited ability to generate cardiomyocytes in vitro and in vivo, even after injury. There is no evidence for Sca-1 expression in embryonic cardiovascular progenitors. In other organs, Sca-1 expression is mainly observed on mesoderm-derived cells, although it is not restricted to stem/progenitor cell populations. It is urgent to determine, at a single cell level, to which extent cardiac Lin-Sca-1+cells overlap with the fibroblast compartment.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84907432293\&partnerID=40\&md5=f861431ef4750c567f4dff89b4bc013c},
	author = {Valente, M. and Nascimento, D.S. and Cumano, A. and Pinto-Do-{\'O}, P.}
}
@article {3306,
	title = {Sensory neurons and osteoblasts: Close partners in a microfluidic platform},
	journal = {Integrative Biology (United Kingdom)},
	volume = {6},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {586 - 595},
	abstract = {Innervation has proven to be critical in bone homeostasis/regeneration due to the effect of soluble factors, produced by nerve fibers, associated with changes in the activity of bone cells. Thus, in this study, we have established and characterized a coculture system comprising sensory neurons and osteoblasts to mimic the in vivo scenario where nerve fibers can be found in a bone microenvironment. Embryonic or adult primary dorsal root ganglion (DRG) and MC3T3-E1 osteoblastic cells were cocultured in compartmentalized microfluidic platforms and morphological and functional tests were performed. The time of adhesion and readout of axonal outgrowth were improved by the alignment of DRG with the axis of microgrooves, which showed to be a crucial step for the designed experiments. Cocultures of entire DRG from adult origin with osteoblasts were performed, showing extended DRG projections towards the axonal compartment, reaching osteoblastic cells. Immunocytochemistry showed that the neurites present within the osteoblastic compartment were immunoreactive to synapsin and calcitonin gene-related peptide suggesting the presence of specialized structures involved in this crosstalk. This evidence was further confirmed by electron microscopy where varicosities were detected as well as electron dense structures in neurite membranes. Aiming to mimic the properties of tissue extracellular matrices, MC3T3-E1 cells were seeded in the axonal side upon laminin, collagen or within 3D functionalized alginate matrices and axonal outgrowth was clearly observed. In order to analyze and quantify data with reproducible image analysis, a semi-automated algorithm was also developed. The collagen and laminin substrates displayed a higher amount of axons reaching the axonal side. Overall, the established method revealed to be a suitable tool to study the interaction between the peripheral nervous system and bone cells in different contexts mimicking the in vivo scenario. {\textcopyright} 2014 the Partner Organisations 2014.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84901624359\&partnerID=40\&md5=0ca57755478aaa339ef3b3cc61479fc3},
	author = {Neto, E. and Alves, C.J. and Sousa, D.M. and Alencastre, I.S. and Louren{\c c}o, A.H. and Leit{\~a}o, L. and Ryu, H.R. and Jeon, N.L. and Fernandes, R. and Aguiar, P. and Almeida, R.D. and Lamghari, M.}
}
@article {3398,
	title = {Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model},
	journal = {Journal of Clinical Investigation},
	volume = {124},
	year = {2014},
	note = {Bestas, Burcu Moreno, Pedro M. D. Blomberg, K. Emelie M. Mohammad, Dara K. Saleh, Amer F. Sutlu, Tolga Nordin, Joel Z. Guterstam, Peter Gustafsson, Manuela O. Kharazi, Shabnam Piatosa, Barbara Roberts, Thomas C. Behlke, Mark A. Wood, Matthew J. A. Gait, Michael J. Lundin, Karin E. El Andaloussi, Samir Mansson, Robert Berglof, Anna Wengel, Jesper Smith, C. I. Edvard},
	month = {2014},
	pages = {4067-4081},
	isbn = {0021-9738},
	url = {<Go to ISI>://WOS:000341168100038},
	author = {Bestas, B. and Moreno, P.M.D. and Blomberg, K. E. M. and Mohammad, D. K. and Saleh, A. F. and Sutlu, T. and Nordin, J. Z. and Guterstam, P. and Gustafsson, M. O. and Kharazi, S. and Piatosa, B. and Roberts, T. C. and Behlke, M. A. and Wood, M. J. A. and Gait, M. J. and Lundin, K.E. and El Andaloussi, S. and Mansson, R. and Berglof, A. and Wengel, J. and Smith, C.I.E.}
}
@article {3283,
	title = {Stable phenotype and function of immortalized Lin-Sca-1 + cardiac progenitor cells in long-term culture: A step closer to standardization},
	journal = {Stem Cells and Development},
	volume = {23},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1012 - 1026},
	abstract = {Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cardiac cell-based therapies. Although two distinct populations of CPCs reached the clinical setting, more detailed studies are required to portray the optimal cell type and therapeutic setting to drive robust cell engraftment and cardiomyogenesis after injury. Owing to the scarcity of the CPCs and the need for reproducibility, the generation of faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized Lin-Sca-1+ CPCs (iCPC Sca-1) represent their native-cell counterpart, thereby constituting a robust in vitro model system for standardized investigation in the cardiac field. iCPCSca-1 were established in vitro as plastic adherent cells endowed with robust self-renewal capacity while preserving a stable phenotype in long-term culture. iCPCSca-1 differentiated into cardiomyocytic-, endothelial-, and smooth muscle-like cells when subjected to appropriate stimuli. The cell line consistently displayed features of Lin -Sca-1+ CPCs in vitro, as well as in vivo after intramyocardial delivery in the onset of myocardial infarction (MI). Transplanted iCPCSca-1 significantly attenuated the functional and anatomical alterations caused by MI while promoting neovascularization. iCPCSca-1 are further shown to engraft, establish functional connections, and differentiate in loco into cardiomyocyte- and vasculature-like cells. These data validate iCPCSca-1 as an in vitro model system for Lin-Sca-1+ progenitors and for systematic dissection of mechanisms underlying CPC subsets engraftment/differentiation in vivo. Moreover, iCPCSca-1 can be regarded as a ready-to-use CPCs source for pre-clinical bioengineering studies toward the development of novel strategies for restoration of the damaged myocardium. {\textcopyright} Copyright 2014, Mary Ann Liebert, Inc. 2014.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896901797\&partnerID=40\&md5=35b7014573b8734d5c21f2089cdc038d},
	author = {Freire, AG and Nascimento, D.S. and Forte, G. and Valente, M. and Resende, TP and Pagliari, S. and Abreu, C and Carvalho, I and Nardo, P.D. and Pinto-Do-{\'O}, P.}
}
@article {3414,
	title = {What do portuguese women prefer regarding vaginal products? Results from a cross-sectional web-based survey},
	journal = {Pharmaceutics},
	volume = {6},
	year = {2014},
	note = {25337676},
	month = {2014},
	pages = {543-56},
	isbn = {1999-4923},
	url = {<Go to ISI>://MEDLINE:25337676},
	author = {Palmeira-de-Oliveira, Rita and Duarte, Paulo and Palmeira-de-Oliveira, Ana and das Neves, Jose and Amaral, Maria Helena and Breitenfeld, Luiza and Martinez-de-Oliveira, Jose}
}
@article {2759,
	title = {Adsorbed fibrinogen leads to improved bone regeneration and correlates with differences in the systemic immune response},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 23 May 2013Source: ScopusArticle in Press},
	month = {07/2013},
	pages = {7209-7217},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876834487\&partnerID=40\&md5=2d00f0bc55f461c9c979548276f0e53d},
	author = {Santos, S.G. and Lamghari, M. and Almeida, C.R. and Oliveira, M.I. and Neves, N. and Ribeiro, A.C. and Barbosa, J.N. and Barros, R. and Maciel, J. and Martins, M.C.L. and Gon{\c c}alves, R.M. and Barbosa, M.A.}
}
@article {2800,
	title = {An overview of central fetal monitoring systems in labour},
	year = {2013},
	month = {2013},
	author = {Nunes, In{\^e}s and Ayres-de-Campos, Diogo and Figueiredo, Catarina and Bernardes, Jo{\~a}o \%@ 1619-3997}
}
@article {2836,
	title = {Brain targeting effect of camptothecin-loaded solid lipid nanoparticles in rat after intravenous administration},
	journal = {European Journal of Pharmaceutics and Biopharmaceutics},
	volume = {85},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {488 - 502},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84888387689\&partnerID=40\&md5=e40e0a0c4221bfbc77f793ace6e47dcd},
	author = {Martins, S.M. and Sarmento, B. and Nunes, C. and L{\'u}cio, M. and Reis, S. and Ferreira, D.C.}
}
@article {2869,
	title = {Cetuximab conjugated O-carboxymethyl chitosan nanoparticles for targeting EGFR overexpressing cancer cells},
	journal = {Carbohydrate Polymers},
	volume = {93},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {661 - 669},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84874136100\&partnerID=40\&md5=71075b389cbeb450f68caf7cbb271c08},
	author = {Maya, S. and Kumar, L.G. and Sarmento, B. and Sanoj Rejinold, N. and Menon, D. and Nair, S.V. and Jayakumar, R.}
}
@article {2885,
	title = {Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {5208 - 5215},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84872069015\&partnerID=40\&md5=22eea6321dd7764e20a4460b47a72ba8},
	author = {Nogueira, F. and Gon{\c c}alves, I.C. and Martins, M.C.L.}
}
@article {2880,
	title = {Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7-10)},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {5643 - 5652},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873199566\&partnerID=40\&md5=f548695261ada2fc57ece26bff1f45d0},
	author = {Amaral, I.F. and Neiva, I. and Ferreira da Silva, F. and Sousa, S.R. and Piloto, A.M. and Lopes, C.D.F. and Barbosa, M.A. and Kirkpatrick, C.J. and P{\^e}go, A.P.}
}
@article {2847,
	title = {Modulation of stability and mucoadhesive properties of chitosan microspheres for therapeutic gastric application},
	journal = {International Journal of Pharmaceutics},
	volume = {454},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {116 - 124},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84884163785\&partnerID=40\&md5=1d040067777b023c63bb769260b66dcd},
	author = {Fernandes, M. and Gon{\c c}alves, I.C. and Nardecchia, S. and Amaral, I.F. and Barbosa, M.A. and Martins, M.C.L.}
}
@article {2886,
	title = {Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability},
	journal = {International Journal of Nanomedicine},
	volume = {8},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {177 - 187},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84872351206\&partnerID=40\&md5=fc5d64a3ada5d6918b225c107d60049e},
	author = {Neves, A.R. and L{\'u}cio, M. and Martins, S. and Lima, J.L.C. and Reis, S.}
}
@article {2874,
	title = {Osteoporotic hip fractures: Bisphosphonates sales and observed turning point in trend. A population-based retrospective study},
	journal = {Bone},
	volume = {53},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {430 - 436},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873288549\&partnerID=40\&md5=da493b87546091513eb8b54c3748cda6},
	author = {Alves, S.M. and Economou, T. and Oliveira, C. and Ribeiro, A.I. and Neves, N. and Gom{\'e}z-Barrena, E. and Pina, M.F.}
}
@article {2887,
	title = {An overview of central fetal monitoring systems in labour},
	journal = {Journal of Perinatal Medicine},
	volume = {41},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {93 - 99},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84881633207\&partnerID=40\&md5=f9c824ddb554c46a92331e20852ef509},
	author = {Nunes, I. and Ayres-de-Campos, D. and Figueiredo, C. and Bernardes, J.}
}
@article {2900,
	title = {Stable phenotype and function of immortalized Lin-Sca-1+ cardiac progenitor cells in long-term culture: a step closer to standardization.},
	journal = {Stem Cells Dev},
	volume = {Epub ahead of print},
	year = {2013},
	month = {12-2013},
	isbn = {PMID: 24367889 },
	url = {http://www.ncbi.nlm.nih.gov/pubmed/24367889},
	author = {Freire, AG and Nascimento, D.S. and Forte, G. and Valente, M. and Resende, TP and Pagliari, S. and Abreu, C and Carvalho, I and Di Nardo, P. and Pinto-Do-{\'O}, P.}
}
@conference {2192,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical ImagingIEEE Comput. Soc. Conf. Comput. Vis. Pattern Recogn.},
	series = {2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012},
	year = {2012},
	note = {Conference code: 91746Export Date: 21 November 2012Source: ScopusArt. No.: 6235546doi: 10.1109/ISBI.2012.6235546Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Lutgens, E., Daemen, M., Muinck, E., Chronic myocardial infarction in the mouse: Cardiac structural and functional changes (1998) Cardiovascular Research, 41, pp. 586-593; 
Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Nascimento, D., Valente, M., Esteves, T., Pina, M.F., Guedes, J.G., Freire, A., Quelhas, P., Pinto-Do, O.P., Miquant - Semi-automation of infarct size assessment in models of cardiac ischemic injury (2011) Plos One, 6 (9), pp. 1-8; 
Esteves, T., Valente, M., Nascimento, D., Pinto-Do, O.P., Quelhas, P., Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model (2011) Proceedings of IbPRIA, LNCS, 6669, pp. 151-158; 
Pfeffer, M., Pfeffer, J., Fishbein, M., Myocardial infarct size and ventricular function in rats (1979) Circulation Research, 44, pp. 503-512
Sponsors: The Institute of Electrical and Electronic Engineers; Engineering in Medicine and Biology Society; Signal Processing Society},
	month = {2012///},
	pages = {310 - 313},
	address = {Barcelona},
	abstract = {Experimental rodent models of induced ischemic injury have been extensively used in biomedical research to study molecular, cellular and histological alterations following myocar-dial infarction. These models are increasingly employed to assess the potential of newly developed therapies for functional restoration of the damaged heart. Such studies are based on myocardial infarction induction followed by different therapeutic interventions and subsequent analysis of the infarct size. This analysis is used to evaluate the extent to which such interventions meet recovery of the lost myocardial tissue. Infarct size is defined as the percentage of the left ventricle affected by coronary artery occlusion. The infarct size is traditionally estimated manually delineating the infarcted and normal tissue areas in the left ventricle of the excised heart. However, this is a time-consuming, arduous and prone to bias process. Herein, we developed an anatomic model, adapted through expectation maximization, which allows for fully automatic analysis of the data. Experimental validation is performed comparing the proposed approach with manual annotation. The results obtained through anatomical model adaptation were coherent with those manually obtained and the differences where never higher than 10\%. {\textcopyright} 2012 IEEE.},
	keywords = {Anatomic models, anatomical model, Anatomical models, Automatic analysis, Biomedical research, Coronary arteries, expectation maximization, Experimental validations, Image segmentation, Infarct size, Ischemic injuries, Left ventricles, Manual annotation, Maximum principle, Medical imaging, Murine model, Myocardial Infarction, Myocardial infarction size, Myocardial tissue, Normal tissue, Rodent models, Therapeutic intervention, Tissue},
	isbn = {19457928 (ISSN); 9781457718588 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=087bcb09e5d5319029623283cba34c34},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@conference {2817,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical Imaging},
	year = {2012},
	note = {Export Date: 2 October 2013Source: ScopusArt. No.: 6235546},
	month = {2012///},
	pages = {310 - 313},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=6716e8eb5d812d3e0ffe54d87369d746},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@article {2819,
	title = {Tissue Engineering and Regenerative Medicine},
	journal = {Tissue Engineering and Regenerative Medicine},
	volume = {6},
	year = {2012},
	month = {2012},
	pages = {110,15.P24},
	publisher = {Blackwell Publishing Ltd},
	url = {http://dx.doi.org/10.1002/term.1586},
	author = {Nascimento D, Silva A, Oliveira MJ Barbosa MA Pinto-do-{\'O} P.}
}
@booklet {2186,
	title = {Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.09.011Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 4150email: cmartins@ineb.up.pt},
	month = {2012///},
	abstract = {Helicobacter pylori infection has been associated with several gastric diseases. This bacterium colonizes the gastric mucosa of half of the world{\textquoteright}s population, and available treatments are unsuccessful in practically one in every five patients. Mucoadhesive polymers, such as chitosan, are being investigated as gastric drug delivery systems. However, since chitosan is also known for its antimicrobial properties, this work aims to evaluate H. pylori interactions with chitosan under simulated gastric environments, namely using various pHs (2.6, 4 and 6), pepsin and urea. To enable the visualization of adherent bacteria, ultrathin chitosan films were produced by spin-coating on gold/glass surfaces, cross-linked with genipin and characterized by Fourier transform infrared reflection absorption spectroscopy, ellipsometry and electrokinetic analysis. Films with homogeneous thickness of 11.7 {\textpm} 0.6 nm were produced, and were stable and protonated at all the pHs used. Furthermore, they adsorbed pepsin in all these pHs, in contrast to urea, of which a small adsorption was only observed at pH 6. H. pylori binding to chitosan was higher at pH 2.6 although most of adherent bacteria were dead. The presence of pepsin decreased bacterial adhesion, but increased its viability while in a more stressed morphology (coccoid form). The presence of urea did not affect the amount, morphology or viability of chitosan-adherent bacteria. In suspension, the decrease in pH changed H. pylori zeta potential from negative to positive. Moreover, bacteria were only culturable when incubated in pH 6 with and without urea (without pepsin). This work demonstrates that chitosan has the capacity to bind and kill H. pylori in a range of pHs independently of urea. This opens new perspectives for the application of chitosan-based materials to the elimination of H. pylori gastric colonization, though pepsin might appear to be an obstacle. {\textcopyright} 2012 Acta Materialia Inc.},
	keywords = {Bacterial adhesion, Biomaterials, chitosan, Helicobacter pylori, Thin films},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867507239\&partnerID=40\&md5=defd903a9833b81e1d4e9e377550ce07},
	author = {Nogueira, F. and Gon{\c c}alves, I.C. and Martins, M.C.L.}
}
@booklet {2179,
	title = {Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII                    7-10)
},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.10.029Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 41email: iamaral@ineb.up.pt},
	month = {2012///},
	abstract = {The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4\% and 15\%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII                        7-10). Immobilized rhFNIII 
                        7-10 was characterized in terms of amount ( 
                        125I-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII 
                        7-10 with rhFNIII 
                        7-10 concentration, and, for the same concentration, higher amounts of rhFNIII 
                        7-10 on DA 4\% compared with DA 15\%. Moreover, rhFNIII 
                        7-10 concentrations as low as 5 and 20 μg ml 
},
	keywords = {Protein conformation, Protein radiolabelling, Spinal cord injury, Surface grafting, Three-dimensional scaffolds},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868680582\&partnerID=40\&md5=6599fd2565f4511b94e944978afc8073},
	author = {Amaral, I.F. and Neiva, I. and Ferreira da Silva, F. and Sousa, S.R. and Piloto, A.M. and Lopes, C.D.F. and Barbosa, M.A. and Kirkpatrick, C.J. and P{\^e}go, A.P.}
}
@article {2797,
	title = {Osteoporotic hip fractures: Bisphosphonates sales and observed turning point in trend. A population-based retrospective study},
	year = {2012},
	month = {2012},
	publisher = {Elsevier},
	author = {Alves, Sandra Maria and Economou, Theodoros and Oliveira, Carla and Ribeiro, Ana Isabel and Neves, Nuno and Gom{\'e}z-Barrena, Enrique and de Pina, Maria de F{\'a}tima}
}
@article {2241,
	title = {The Arabidopsis D-Type Cyclin CYCD2;1 and the Inhibitor ICK2/KRP2 Modulate Auxin-Induced Lateral Root Formation},
	journal = {Plant CellPlant Cell},
	volume = {23},
	year = {2011},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: ScopusCODEN: PLCEEdoi: 10.1105/tpc.110.080002PubMed ID: 21357490Language of Original Document: EnglishCorrespondence Address: Murray, J. A. H.; Cardiff School of Biosciences, Cardiff University, CF10 3AX Cardiff, United Kingdom; email: murrayja1@cardiff.ac.ukChemicals/CAS: proteasome, 140879-24-9; ATP dependent 26S protease, 3.4.99.-; Arabidopsis Proteins; Cell Cycle Proteins; CycD2 protein, Arabidopsis; Cyclins; ICK2 protein, Arabidopsis; Indoleacetic Acids; Proteasome Endopeptidase Complex, 3.4.25.1References: Achard, P., Gusti, A., Cheminant, S., Alioua, M., Dhondt, S., Coppens, F., Beemster, G.T., Genschik, P., Gibberellin signaling controls cell proliferation rate in Arabidopsis (2009) Curr. Biol., 19, pp. 1188-1193;Alonso, J.M., Genome-wide insertional mutagenesis of Arabidopsis thaliana (2003) Science, 301, pp. 653-657; 
Baluska, F., Mancuso, S., Volkmann, D., Barlow, P.W., Root apex transition zone: A signalling-response nexus in the root (2010) Trends Plant Sci., 15, pp. 402-408; 
Bechtold, N., Pelletier, G., In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration (1998) Methods Mol. Biol., 82, pp. 259-266; 
Bird, D.A., Buruiana, M.M., Zhou, Y., Fowke, L.C., Wang, H., Arabidopsis cyclin-dependent kinase inhibitors are nuclearlocalized and show different localization patterns within the nucleoplasm (2007) Plant Cell Rep., 26, pp. 861-872; 
Boerjan, W., Cervera, M.T., Delarue, M., Beeckman, T., Dewitte, W., Bellini, C., Caboche, M., Inz{\'e}, D., Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction (1995) Plant Cell, 7, pp. 1405-1419; 
Boisnard-Lorig, C., Colon-Carmona, A., Bauch, M., Hodge, S., Doerner, P., Bancharel, E., Dumas, C., Berger, F., Dynamic analyses of the expression of the HISTONE:YFP fusion protein in Arabidopsis show that syncytial endosperm is divided in mitotic domains (2001) Plant Cell, 13, pp. 495-509; 
Boniotti, M.B., Gutierrez, C., A cell-cycle-regulated kinase activity phosphorylates plant retinoblastoma protein and contains, in Arabidopsis, a CDKA/cyclin D complex (2001) Plant J., 28, pp. 341-350; 
Boruc, J., Van den Daele, H., Hollunder, J., Rombauts, S., Mylle, E., Hilson, P., Inz{\'e}, D., Russinova, E., Functional modules in the Arabidopsis core cell cycle binary proteinprotein interaction network (2010) Plant Cell, 22, pp. 1264-1280; 
Brady, S.M., Orlando, D.A., Lee, J.Y., Wang, J.Y., Koch, J., Dinneny, J.R., Mace, D., Benfey, P.N., A high-resolution root spatiotemporal map reveals dominant expression patterns (2007) Science, 318, pp. 801-806; 
Casimiro, I., Beeckman, T., Graham, N., Bhalerao, R., Zhang, H., Casero, P., Sandberg, G., Bennett, M.J., Dissecting Arabidopsis lateral root development (2003) Trends Plant Sci., 8, pp. 165-171; 
Casimiro, I., Marchant, A., Bhalerao, R.P., Beeckman, T., Dhooge, S., Swarup, R., Graham, N., Bennett, M., Auxin transport promotes Arabidopsis lateral root initiation (2001) Plant Cell, 13, pp. 843-852; 
Chevray, P.M., Nathans, D., Protein interaction cloning in yeast: Identification of mammalian proteins that react with the leucine zipper of Jun (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 5789-5793; 
Churchman, M.L., SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana (2006) Plant Cell, 18, pp. 3145-3157; 
Cleary, A., Fowke, L., Wang, H., John, P., The effect of ICK1, a plant cyclin-dependent kinase inhibitor, on mitosis in living plant cells (2002) Plant Cell Rep., 20, pp. 814-820; 
Clough, S.J., Bent, A.F., Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana (1998) Plant J., 16, pp. 735-743; 
Cockcroft, C.E., den Boer, B.G., Healy, J.M., Murray, J.A.H., Cyclin D control of growth rate in plants (2000) Nature, 405, pp. 575-579; 
de Smet, I., Vanneste, S., Inz{\'e}, D., Beeckman, T., Lateral root initiation or the birth of a new meristem (2006) Plant Mol. Biol., 60, pp. 871-887; 
de Smet, I., Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis (2007) Development, 134, pp. 681-690; 
de Smet, I., Bimodular auxin response controls organogenesis (2010) Proc. Natl. Acad. Sci. USA, 107, pp. 2705-2710; 
de Veylder, L., Beeckman, T., Beemster, G.T., de Almeida Engler, J., Ormenese, S., Maes, S., Naudts, M., Inz{\'e}, D., Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor (2002) EMBO J., 21, pp. 1360-1368; 
de Veylder, L., Beeckman, T., Beemster, G.T., Krols, L., Terras, F., Landrieu, I., van der Schueren, E., Inz{\'e}, D., Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis (2001) Plant Cell, 13, pp. 1653-1668; 
de Veylder, L., de Almeida Engler, J., Burssens, S., Manevski, A., Lescure, B., Van Montagu, M., Engler, G., Inz{\'e}, D., A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation (1999) Planta, 208, pp. 453-462; 
Dewitte, W., Murray, J.A.H., The plant cell cycle (2003) Annu. Rev. Plant Biol., 54, pp. 235-264; 
Dewitte, W., Riou-Khamlichi, C., Scofield, S., Healy, J.M., Jacqmard, A., Kilby, N.J., Murray, J.A.H., Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3 (2003) Plant Cell, 15, pp. 79-92; 
Dewitte, W., Scofield, S., Alcasabas, A.A., Maughan, S.C., Menges, M., Braun, N., Collins, C., Murray, J.A.H., Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses (2007) Proc. Natl. Acad. Sci. USA, 104, pp. 14537-14542; 
Dharmasiri, N., Dharmasiri, S., Weijers, D., Karunarathna, N., Jurgens, G., Estelle, M., AXL and AXR1 have redundant functions in RUB conjugation and growth and development in Arabidopsis (2007) Plant J., 52, pp. 114-123; 
Ditengou, F.A., Teale, W.D., Kochersperger, P., Flittner, K.A., Kneuper, I., van der Graaff, E., Nziengui, H., Palme, K., Mechanical induction of lateral root initiation in Arabidopsis thaliana (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 18818-18823; 
Dubrovsky, J.G., Rost, T.L., Col{\'o}n-Carmona, A., Doerner, P., Early primordium morphogenesis during lateral root initiation in Arabidopsis thaliana (2001) Planta, 214, pp. 30-36; 
Dubrovsky, J.G., Sauer, M., Napsucialy-Mendivil, S., Ivanchenko, M.G., Friml, J., Shishkova, S., Celenza, J., Benkov{\'a}, E., Auxin acts as a local morphogenetic trigger to specify lateral root founder cells (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 8790-8794; 
Duttweiler, H.M., A highly sensitive and non-lethal beta-galactosidase plate assay for yeast (1996) Trends Genet., 12, pp. 340-341; 
Ebel, C., Mariconti, L., Gruissem, W., Plant retinoblastoma homologues control nuclear proliferation in the female gametophyte (2004) Nature, 429, pp. 776-780; 
Fukaki, H., Tameda, S., Masuda, H., Tasaka, M., Lateral root formation is blocked by a gain-of-function mutation in the SOLITARY-ROOT/IAA14 gene of Arabidopsis (2002) Plant J., 29, pp. 153-168; 
Fukaki, H., Tasaka, M., Hormone interactions during lateral root formation (2009) Plant Mol. Biol., 69, pp. 437-449; 
Healy, J.M., Menges, M., Doonan, J.H., Murray, J.A.H., The Arabidopsis D-type cyclins CycD2 and CycD3 both interact in vivo with the PSTAIRE cyclin-dependent kinase Cdc2a but are differentially controlled (2001) J. Biol. Chem., 276, pp. 7041-7047; 
Himanen, K., Boucheron, E., Vanneste, S., de Almeida Engler, J., Inz{\'e}, D., Beeckman, T., Auxin-mediated cell cycle activation during early lateral root initiation (2002) Plant Cell, 14, pp. 2339-2351; 
Himanen, K., Vuylsteke, M., Vanneste, S., Vercruysse, S., Boucheron, E., Alard, P., Chriqui, D., Beeckman, T., Transcript profiling of early lateral root initiation (2004) Proc. Natl. Acad. Sci. USA, 101, pp. 5146-5151; 
Hirano, H., Harashima, H., Shinmyo, A., Sekine, M., Arabidopsis RETINOBLASTOMA-RELATED PROTEIN 1 is involved in G1 phase cell cycle arrest caused by sucrose starvation (2008) Plant Mol. Biol., 66, pp. 259-275; 
Huntley, R., The maize retinoblastoma protein homologue ZmRb-1 is regulated during leaf development and displays conserved interactions with G1/S regulators and plant cyclin D (CycD) proteins (1998) Plant Mol. Biol., 37, pp. 155-169; 
Inz{\'e}, D., De Veylder, L., Cell cycle regulation in plant development (2006) Annu. Rev. Genet., 40, pp. 77-105; 
Jakoby, M.J., Weinl, C., Pusch, S., Kuijt, S.J., Merkle, T., Dissmeyer, N., Schnittger, A., Analysis of the subcellular localization, function, and proteolytic control of the Arabidopsis cyclin-dependent kinase inhibitor ICK1/KRP1 (2006) Plant Physiol., 141, pp. 1293-1305; 
James, P., Halladay, J., Craig, E.A., Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast (1996) Genetics, 144, pp. 1425-1436; 
Jasinski, S., Riou-Khamlichi, C., Roche, O., Perennes, C., Bergounioux, C., Glab, N., The CDK inhibitor NtKIS1a is involved in plant development, endoreduplication and restores normal development of cyclin D3;1-overexpressing plants (2002) J. Cell Sci., 115, pp. 973-982; 
Karimi, M., Depicker, A., Hilson, P., Recombinational cloning with plant gateway vectors (2007) Plant Physiol., 145, pp. 1144-1154; 
Kohalmi, S.E., Nowak, J., Crosby, W.L., The yeast twohybrid system (1997) Differentially Expressed Genes in Plants: A Bench Manual, pp. 63-82. , In, E. Hansen and G. Harper, eds (London: Taylor \& Francis); 
Laskowski, M., Grieneisen, V.A., Hofhuis, H., Hove, C.A., Hogeweg, P., Mar{\'e}e, A.F., Scheres, B., Root system architecture from coupling cell shape to auxin transport (2008) PLoS Biol., 6, pp. e307; 
Laskowski, M.J., Williams, M.E., Nusbaum, H.C., Sussex, I.M., Formation of lateral root meristems is a two-stage process (1995) Development, 121, pp. 3303-3310; 
Lechner, E., Xie, D., Grava, S., Pigaglio, E., Planchais, S., Murray, J.A.H., Parmentier, Y., Genschik, P., The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects (2002) J. Biol. Chem., 277, pp. 50069-50080; 
Lincoln, C., Britton, J.H., Estelle, M., Growth and development of the axr1 mutants of Arabidopsis (1990) Plant Cell, 2, pp. 1071-1080; 
L{\'o}pez-Bucio, J., Cruz-Ram{\'\i}rez, A., Herrera-Estrella, L., The role of nutrient availability in regulating root architecture (2003) Curr. Opin. Plant Biol., 6, pp. 280-287; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated Arabidopsis plant root cell segmentation based on SVM classification and region merging (2009) Comput. Biol. Med., 39, pp. 785-793; 
Masubelele, N.H., Dewitte, W., Menges, M., Maughan, S., Collins, C., Huntley, R., Nieuwland, J., Murray, J.A.H., D-type cyclins activate division in the root apex to promote seed germination in Arabidopsis (2005) Proc. Natl. Acad. Sci. USA, 102, pp. 15694-15699; 
Menges, M., de Jager, S.M., Gruissem, W., Murray, J.A.H., Global analysis of the core cell cycle regulators of Arabidopsis identifies novel genes, reveals multiple and highly specific profiles of expression and provides a coherent model for plant cell cycle control (2005) Plant J., 41, pp. 546-566; 
Menges, M., Samland, A.K., Planchais, S., Murray, J.A.H., The D-type cyclin CYCD3;1 is limiting for the G1-to-S-phase transition in Arabidopsis (2006) Plant Cell, 18, pp. 893-906; 
Minet, M., Dufour, M.E., Lacroute, F.B., Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs (1992) Plant J., 2, pp. 417-422; 
Moreno-Risueno, M.A., Van Norman, J.M., Moreno, A., Zhang, J., Ahnert, S.E., Benfey, P.N., Oscillating gene expression determines competence for periodic Arabidopsis root branching (2010) Science, 329, pp. 1306-1311; 
Nieuwland, J., Maughan, S., Dewitte, W., Scofield, S., Sanz, L., Murray, J.A.H., The D-type cyclin CYCD4;1 modulates lateral root density in Arabidopsis by affecting the basal meristem region (2009) Proc. Natl. Acad. Sci. USA, 106, pp. 22528-22533; 
Okushima, Y., Fukaki, H., Onoda, M., Theologis, A., Tasaka, M., ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis (2007) Plant Cell, 19, pp. 118-130; 
P{\'e}ret, B., De Rybel, B., Casimiro, I., Benkov{\'a}, E., Swarup, R., Laplaze, L., Beeckman, T., Bennett, M.J., Arabidopsis lateral root development: An emerging story (2009) Trends Plant Sci., 14, pp. 399-408; 
Petersson, S.V., Johansson, A.I., Kowalczyk, M., Makoveychuk, A., Wang, J.Y., Moritz, T., Grebe, M., Ljung, K., An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis (2009) Plant Cell, 21, pp. 1659-1668; 
Petroski, M.D., Deshaies, R.J., Function and regulation of cullin-RING ubiquitin ligases (2005) Nat. Rev. Mol. Cell Biol., 6, pp. 9-20; 
Planchais, S., Samland, A.K., Murray, J.A.H., Differential stability of Arabidopsis D-type cyclins: CYCD3;1 is a highly unstable protein degraded by a proteasome-dependent mechanism (2004) Plant J., 38, pp. 616-625; 
Qi, R., John, P.C., Expression of genomic AtCYCD2;1 in Arabidopsis induces cell division at smaller cell sizes: Implications for the control of plant growth (2007) Plant Physiol., 144, pp. 1587-1597; 
Ren, H., Santner, A., del Pozo, J.C., Murray, J.A.H., Estelle, M., Degradation of the cyclin-dependent kinase inhibitor KRP1 is regulated by two different ubiquitin E3 ligases (2008) Plant J., 53, pp. 705-716; 
Richter, G.L., Monshausen, G.B., Krol, A., Gilroy, S., Mechanical stimuli modulate lateral root organogenesis (2009) Plant Physiol., 151, pp. 1855-1866; 
Riou-Khamlichi, C., Huntley, R., Jacqmard, A., Murray, J.A.H., Cytokinin activation of Arabidopsis cell division through a D-type cyclin (1999) Science, 283, pp. 1541-1544; 
Riou-Khamlichi, C., Menges, M., Healy, J.M., Murray, J.A.H., Sugar control of the plant cell cycle: Differential regulation of Arabidopsis D-type cyclin gene expression (2000) Mol. Cell. Biol., 20, pp. 4513-4521; 
Sabatini, S., Beis, D., Wolkenfelt, H., Murfett, J., Guilfoyle, T., Malamy, J., Benfey, P., Scheres, B., An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root (1999) Cell, 99, pp. 463-472; 
Schnittger, A., Sch{\"o}binger, U., Bouyer, D., Weinl, C., Stierhof, Y.D., H{\"u}lskamp, M., Ectopic D-type cyclin expression induces not only DNA replication but also cell division in Arabidopsis trichomes (2002) Proc. Natl. Acad. Sci. USA, 99, pp. 6410-6415; 
van Leene, J., Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana (2010) Mol. Syst. Biol., 6, p. 397; 
Vanneste, S., Cell cycle progression in the pericycle is not sufficient for SOLITARY ROOT/IAA14-mediated lateral root initiation in Arabidopsis thaliana (2005) Plant Cell, 17, pp. 3035-3050; 
Verkest, A., Manes, C.L., Vercruysse, S., Maes, S., van der Schueren, E., Beeckman, T., Genschik, P., De Veylder, L., The cyclin-dependent kinase inhibitor KRP2 controls the onset of the endoreduplication cycle during Arabidopsis leaf development through inhibition of mitotic CDKA;1 kinase complexes (2005) Plant Cell, 17, pp. 1723-1736; 
Verkest, A., Weinl, C., Inz{\'e}, D., De Veylder, L., Schnittger, A., Switching the cell cycle. Kip-related proteins in plant cell cycle control (2005) Plant Physiol., 139, pp. 1099-1106; 
Wang, H., Qi, Q., Schorr, P., Cutler, A.J., Crosby, W.L., Fowke, L.C., ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid (1998) Plant J., 15, pp. 501-510; 
Wildwater, M., Campilho, A., Perez-Perez, J.M., Heidstra, R., Blilou, I., Korthout, H., Chatterjee, J., Scheres, B., The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots (2005) Cell, 123, pp. 1337-1349; 
Willemse, J., Kulikova, O., de Jong, H., Bisseling, T., A new whole-mount DNA quantification method and the analysis of nuclear DNA content in the stem-cell niche of Arabidopsis roots (2008) Plant J., 55, pp. 886-894; 
Winter, D., Vinegar, B., Nahal, H., Ammar, R., Wilson, G.V., Provart, N.J., An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets (2007) PLoS ONE, 2, pp. e718; 
Zhou, Y., Fowke, L.C., Wang, H., Plant CDK inhibitors: Studies of interactions with cell cycle regulators in the yeast twohybrid system and functional comparisons in transgenic Arabidopsis plants (2002) Plant Cell Rep., 20, pp. 967-975; 
Zhou, Y., Niu, H., Brandizzi, F., Fowke, L.C., Wang, H., Molecular control of nuclear and subnuclear targeting of the plant CDK inhibitor ICK1 and ICK1-mediated nuclear transport of CDKA (2006) Plant Mol. Biol., 62, pp. 261-278; 
Zhou, Y., Wang, H., Gilmer, S., Whitwill, S., Fowke, L.C., Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana (2003) Planta, 216, pp. 604-613
},
	month = {2011///},
	pages = {641 - 660},
	abstract = {The integration of cell division in root growth and development requires mediation of developmental and physiological signals through regulation of cyclin-dependent kinase activity. Cells within the pericycle form de novo lateral root meristems, and D-type cyclins (CYCD), as regulators of the G                        1-to-S phase cell cycle transition, are anticipated to play a role. Here, we show that the D-type cyclin protein CYCD2;1 is nuclear in Arabidopsis thaliana root cells, with the highest concentration in apical and lateral meristems. Loss of CYCD2;1 has a marginal effect on unstimulated lateral root density, but CYCD2;1 is rate-limiting for the response to low levels of exogenous auxin. However, while CYCD2;1 expression requires sucrose, it does not respond to auxin. The protein Inhibitor-Interactor of CDK/Kip Related Protein2 (ICK2/KRP2), which interacts with CYCD2;1, inhibits lateral root formation, and ick2/krp2 mutants show increased lateral root density. ICK2/ KRP2 can modulate the nuclear levels of CYCD2;1, and since auxin reduces ICK2/KRP2 protein levels, it affects both activity and cellular distribution of CYCD2;1. Hence, as ICK2/KRP2 levels decrease, the increase in lateral root density depends on CYCD2;1, irrespective of ICK2/CYCD2;1 nuclear localization. We propose that ICK2/KRP2 restrains root ramification by maintaining CYCD2;1 inactive and that this modulates pericycle responses to auxin fluctuations. {\textcopyright} 2011 American Society of Plant Biologists.
},
	keywords = {Arabidopsis, Arabidopsis protein, Arabidopsis Proteins, Arabidopsis thaliana, article, ATP dependent 26S protease, cell cycle, cell cycle protein, Cell Cycle Proteins, cell nucleus, CycD2 protein, Arabidopsis, cycline, Cyclins, gene expression regulation, Gene Expression Regulation, Plant, genetics, growth, development and aging, ICK2 protein, Arabidopsis, indoleacetic acid derivative, Indoleacetic Acids, metabolism, mutation, plant root, Plant roots, proteasome, Proteasome Endopeptidase Complex},
	isbn = {10404651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953123085\&partnerID=40\&md5=f1355f64db2bcf9f2d881104f23005eb},
	author = {Sanz, L. and Dewitte, W. and Forzani, C. and Patell, F. and Nieuwland, J. and Wen, B. and Quelhas, P. and de Jager, S. and Titmus, C. and Campilho, A. and Ren, H. and Estelle, M. and Wang, H. and Murray, J.A.H.}
}
@book {2222,
	title = {Arabidopsis thaliana automatic cell file detection and cell length estimation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6754 LNCS},
	year = {2011},
	note = {Conference code: 85546Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21596-4_1Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB - Divis{\~a}o de Sinal e Imagem, Instituto de Engenharia Biom{\'e}dica, Porto, PortugalReferences: Terpstra, I., Heidstra, R., Stem cells: The root of all cells (2009) Semin. Cell Dev. Biol., 20 (9), pp. 1089-1096;Bennett, T., Scheres, B., Root development-two meristems for the price of one? (2010) Curr. Top Dev. Bio., 91, pp. 67-102; 
De Smet, I., Tetsumura, T., De Rybel, B., Frey, N.F., Laplaze, L., Casimiro, I., Swarup, R., Beeckman, T., Auxin-dependent regulation of lateral root positioning in the basal meristem of arabidopsis (2007) Development, 134 (4), pp. 681-690; 
Nieuwland, J., Maughan, S., Dewitte, W., Scofield, S., Sanz, L., Murray, J.A., The d-type cyclin cycd4;1 modulates lateral root density in arabidopsis by affecting the basal meristem region (2009) Proc. Natl. Acad. Sci. U S A, 106 (52), pp. 22528-22533; 
Vanstraelen, M., Baloban, M., Da Ines, O., Cultrone, A., Lammens, T., Boudolf, V., Brown, S.C., Kondorosi, E., Apc/c-ccs52a complexes control meristem maintenance in the arabidopsis root (2009) Proc. Natl. Acad. Sci. U S A, 106 (28), pp. 11806-11811; 
Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res., 119, pp. 85-93; 
Immink, R., Gadella, T., Ferrario, S., Busscher, M., Angenent, G., Analysis of mads box protein-protein interactions in living plant cells (2002) Proc. Natl. Acad. Sci. USA, 99, pp. 2416-2421; 
Willemse, J., Kulikova, O., Jong, H., Bisseling, T., A new whole-mount dna quantification method and the analysis of nuclear dna content in the stem-cell niche of arabidopsis roots (2008) The Plant Journal, 10, pp. 1365-1374; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Comp. in Biology and Medicine, 39, pp. 785-793; 
Marcuzzo, M., Guichard, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Araujo, H., Mendon{\c c}a, A.M., Pinho, A.J., Torres, M.I. (eds.) IbPRIA 2009. Springer, Heidelberg; 
Kovesi, P., Symmetry and asymmetry from local phase Proc. of the 10th Australian Joint Conf. on Artificial Intelligence (December 1997); 
Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Transactions on Systems, Man, and Cybernetics, SMC-9 (1), pp. 62-66
},
	month = {2011///},
	pages = {1 - 11},
	address = {Burnaby, BC},
	abstract = {In plant development biology, the study of the structure of the plant{\textquoteright}s root is fundamental for the understanding of the regulation and interrelationships of cell division and cellular differentiation. This is based on the high connection between cell length and progression of cell differentiation and the nuclear state. However, the need to analyse a large amount of images from many replicate roots to obtain reliable measurements motivates the development of automatic tools for root structure analysis. We present a novel automatic approach to detect cell files, the main structure in plant roots, and extract the length of the cells in those files. This approach is based on the detection of local cell file characteristic symmetry using a wavelet based image symmetry measure. The resulting detection enables the automatic extraction of important data on the plant development stage and of characteristics for individual cells. Furthermore, the approach presented reduces in more than 90\% the time required for the analysis of each root, improving the work of the biologist and allowing the increase of the amount of data to be analysed for each experimental condition. While our approach is fully automatic a user verification and editing stage is provided so that any existing errors may be corrected. Given five test images it was observed that user did not correct more than 20\% of all automatically detected structure, while taking no more than 10\% of manual analysis time to do so. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Automatic extraction, Automatic tools, Bio-imaging, BioImaging, cell differentiation, Cell divisions, Cell lengths, cell proliferation, Cells, Cellular differentiation, Experimental conditions, Image analysis, image symmetry, Individual cells, Main structure, Manual analysis, Nuclear state, Plant development, Plant roots, Plants (botany), Reliable measurement, Root structure, Test images, User verification, Wavelet analysis, Wavelet-based images},
	isbn = {03029743 (ISSN); 9783642215957 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960337749\&partnerID=40\&md5=ef49700082788511730e38d4eda9a566},
	author = {Quelhas, P. and Nieuwland, J. and Dewitte, W. and Mendon{\c c}a, A.M. and Murray, J. and Campilho, A.}
}
@book {2224,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6669 LNCS},
	year = {2011},
	note = {Conference code: 85429Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21257-4_19Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: meb09026@fe.up.ptReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Alattar, M., Osman, N., Fahmy, A., Myocardial segmentation using constrained multi-seeded region growing (2010) LNCS, 6112, pp. 89-98. , Campilho, A., Kamel, M. (eds.) ICIAR 2010 Springer, Heidelberg; 
Wu, Q., Merchant, F., Castleman, K., (1996) Microscope Image Processing, , ch. 7. Elsevier, Amsterdam; 
Gonzalez, R., Woods, R., Eddins, S., (2004) Digital Image Processing Using MATLAB, , ch. 9. Pearson Education, London; 
Sharma, N., Aggarwal, L., Automated medical image segmentation techniques (2010) Journal of Medical Physics, 35 (1), pp. 3-14; 
Pham, D., Xu, C., A survey of current methods in medical image segmentation (1998) Annual Review of Biomedical Engineering, 2, pp. 315-338; 
Hamarneh, G., Li, X., Watershed segmentation using prior shape and appearance knowledge (2009) Image and Vision Comp, 27 (1-2), pp. 59-68; 
Khadir, S., Ahamed, R., Moving toward region-based image segmentation techniques: A study (2009) Journal of Theoretical and Applied Information Technology, 5 (1), pp. 1-7; 
Ahmed, M., Mohamad, D., Segmentation of brain mr images for tumor extraction by combining kmeans clustering and perona-malik anisotropic diffusion model (2010) International Journal of Image Processing, 2 (1), pp. 1-8; 
Mayer, A., Greenspan, H., An adaptive mean-shift framework for MRI brain segmentation (2009) IEEE Trans. on Medical Imaging, 28 (8), pp. 1-12; 
Dabov, K., Foi, A., Katkovnik, V., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 1-16; 
Otsu, N., A threshold selection method from gray level histograms (1979) IEEE Trans. Systems, Man and Cybernetics, 9 (1), pp. 62-66; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Computers in Biology and Medicine, 39 (9), pp. 1-9; 
Bleau, A., Leon, J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370
},
	month = {2011///},
	pages = {151 - 158},
	address = {Las Palmas de Gran Canaria},
	abstract = {Rodent models of myocardial infarction (MI) have been extensively used in biomedical research towards the implementation of novel regenerative therapies. Permanent ligation of the left anterior descending (LAD) coronary artery is a commonly used method for inducing MI both in rat and mouse. Post-mortem evaluation of the heart, particularly the MI extension assessment performed on histological sections, is a critical parameter for this experimental setting. MI extension, which is defined as the percentage of the left ventricle affected by the coronary occlusion, has to be estimated by identifying the infarcted- and the normal-tissue in each section. However, because it is a manual procedure it is time-consuming, arduous and prone to bias. Herein, we introduce semi-automatic and automatic approaches to perform segmentation which is then used to obtain the infarct extension measurement. Experimental validation is performed comparing the proposed approaches with manual annotation and a total error not exceeding 8\% is reported in all cases. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Extension evaluations, Image analysis, Image segmentation, Infarct extension evaluation, k-means, Mammals, meanshift, otsu, Pattern recognition, region growing, Tissue, watershed},
	isbn = {03029743 (ISSN); 9783642212567 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=360bb21a8c1c3a5fdd394c647fcadbbc},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@book {2818,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)},
	volume = {6669 LNCS},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: Scopus},
	month = {2011///},
	pages = {151 - 158},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=58726bd7fc58cf587a13af9e3ba0c151},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@article {2815,
	title = {MIQuant - semi-automation of infarct size assessment in models of cardiac ischemic injury},
	journal = {PLoS ONE},
	volume = {6},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: ScopusArt. No.: e25045},
	month = {2011///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80053583980\&partnerID=40\&md5=757f7f25dcffa46f5fd66ccee74f13f0},
	author = {Nascimento, D.S. and Valente, M. and Esteves, T. and De F{\'a}tima De Pina, M. and Guedes, J.G. and Freire, A. and Quelhas, P. and Pinto-Do-{\'O}, P.}
}
@article {2265,
	title = {Characterization of two DLC coatings for joint prosthesis: The role of albumin on the tribological behavior},
	journal = {Surface and Coatings TechnologySurf. Coat. Technol.},
	volume = {204},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.surfcoat.2010.04.022Language of Original Document: EnglishCorrespondence Address: Serro, A.P.; Centro de Qu{\'\i}mica Estrutural, Complexo I, Instituto Superior T{\'e}cnico, Av. Rovisco Pais, TU Lisbon, 1049-001 Lisboa, Portugal; email: anapaula.serro@ist.utl.ptReferences: Donnet, C., Erdemir, A., (2009), Springer Science \& Business Media, New YorkGutensohn, K., Beythien, C., Bau, J., Fenner, T., Grewe, P., Koester, R., Padmanaban, K., Kuehnl, P., (2000) Thromb. Res., 99, p. 577;Cadosch, D., Chan, E., Gautschi, O.P., Simmen, H.-P., Filgueira, L., (2009) J. Orthop. Res., 27, p. 841; 
Gillespie, W.L., Frampton, C.M.A., Henderson, R.J., Ryan, P.M., (1988) J. Bone Joint Surg. Br., 70 (4), p. 539; 
Roy, R.K., Lee, K.-R., (2007) J. Biomed. Mater. Res. B Appl. Biomater., 83 B (1), p. 72; 
Alakoski, E., Tiainen, V.-M., Soininen, A., Konttinen, Y.T., (2008) Open Orthop. J., 2, p. 43; 
Fisher, J., Firkins, P., Reeves, E.A., Hailey, J.L., Isaac, G.H., (1995) Proc. Inst. Mech. Eng., 209, p. 263; 
Sheeja, D., Tay, B.K., Lau, S.P., Nung, L.N., (2001) Surf. Coat. Technol., p. 410; 
Tiainen, V.-M., (2001) Diamond Relat. Mater., 10 (2), p. 153; 
O{\~n}ate, J.I., Comin, M., Braceras, I., Garcia, A., Viviente, J.L., Brizuela, M., Garagorri, N., Alava, J.I., (2001) Surf. Coat. Technol., p. 1056; 
Shi, B., Ajayi, O.O., Fenske, G., Erdemir, A., Liang, H., (2003) Wear, 255, p. 1015; 
Lappalainen, R., Selenius, M., Anttila, A., Konttinen, Y.T., Santavirta, S.S., (2003) J. Appl. Biomater., 66 B, p. 410; 
Sheeja, D., Tay, B.K., Nung, L.N., (2004) Diamond Relat. Mater., 13, p. 184; 
Xu, T., Pruitt, L., (1999) J. Mater. Sci. Mater. Med., 10, p. 83; 
Lappalainen, R., Anttila, A., Heinonen, H., (1998) Clin. Orthop., 352, p. 118; 
Saikko, V., Ahlroos, T., Calonius, O., Ker{\"a}nen, J., (2001) Biomaterials, 22, p. 1507; 
Sheeja, D., Tay, B.K., Lau, S.P., Nung, L.N., (2001) Surf. Coat. Technol., 146, p. 410; 
Affatato, S., Frigo, M., Toni, A., (2000) J. Biomed. Mater. Res., 53 (3), p. 221; 
Scholes, S.C., Unsworth, A., Goldsmit, A.A.J., (2000) Phys. Med. Biol., 45, p. 3721; 
Ahlroos, T., Saikko, V., (1997) Wear, 211, p. 113; 
Fedel, M., Motta, A., Maniglio, D., (2009) J. Biomed. Mater. Res., 90 B (1), p. 338; 
Lousiniana, S., Logothetidis, S., Laskarakisa, A., Gioti, M., (2007) Biomolec. Eng., 24 (1), p. 107; 
Li, B.G., Kang, Y.Q., Yin, G.F., Zheng, C.Q., (2007) Key Eng. Mater. High-Performance Ceram. IV, p. 1814; 
Fenske, G.R., (2009) Presented to Chicago Section STLE meeting, , M; 
Eryilmaz, O.L., Erdemir, A., (2008) Surf. Coat. Technol., 203, p. 750; 
Gerwin, N., Hops, C., Lucke, A., (2006) Adv. Drug Deliv. Rev., 58 (2), p. 226; 
Visco, A.M., Torrisi, L., Campo, N., Emanuele, U., Trifiro, A., Trimarchi, M., (2009) J. Biomed. Mater. Res. B Appl. Biomater., 89 B, p. 55; 
Ferrari, A.C., Robertson, J., (2001) Phys. Rev. B, 64, p. 75414; 
Ferrari, A.C., Robertson, J., (2000) Phys. Rev. B, 61, p. 14095; 
Zhou, B., Prorok, B.C., Erdemir, A., Eryilmaz, O., (2007) Diamond Relat. Mater., 16, p. 342; 
Zhou, B., Prorok, B.C., Erdemir, A., Eryilmaz, O., (2006) Diamond Relat. Mater., 15, p. 2051; 
Beeman, D., Silverman, J., Lynds, R., Anderson, M.R., (1984) Phys. Rev. B., 30 (2), p. 870; 
Cola{\c c}o, R., Serro, A.P., Eryilmaz, O.L., Erdemir, E., (2009) J. Phys. D Appl. Phys., 42, p. 85307; 
Paul, R., Das, S.N., Dalui, S., Gayen, R.N., Roy, R.K., Bhar, R., Pal, A.K., (2008) J. Phys. D Appl. Phys., 41 (5), p. 55309; 
Serro, A.P., Fernandes, A.C., Saramago, B., (1997) Colloids Surf. A Physicochem. Eng. Aspects, 125, p. 209; 
Serro, A.P., Degiampietro, K., Cola{\c c}o, R., Saramago, B., (2010) Saramago, Adsorption of albumin and sodium hyaluronate on UHMWPE: A QCM-D and AFM study, Colloids and Surfaces B: Biointerfaces, 78, p. 1; 
de Feijter, J.A., Benjamins, J., Veer, F.A., (1978) Biopolymers, 17, p. 1759; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., (2008) Surf. Int. Anal., 40 (12), p. 1529; 
Hook, F., Kasemo, B., Nylander, T., Fant, C., Sott, K., Elwing, H., (2001) Anal. Chem., 73 (24), p. 5796; 
Hook, F., Voros, J., Rodahl, M., Kurrat, R., Boni, P., Ramsden, J.J., Textor, M., Kasemo, B., (2002) Colloids Surf. B Biointerfaces, 24, p. 155; 
Cola{\c c}o, R., Gispert, M.P., Serro, A.P., Saramago, B., (2007) Tribol. Lett., 26 (2), p. 119; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., (2006) Wear, 260, p. 149; 
Serro, A.P., Completo, C., Cola{\c c}o, R., Dos Santos, F., Lobato da Silva, C., Cabral, J.M.S., Ara{\'u}jo, H., Saramago, B., (2009) Surf. Coat. Technol., 203, p. 3701; 
Widmer, M.R., Heuberger, M., V{\"o}r{\"o}s, J., Spencer, N.D., (2001) Tribol. Lett., 10, p. 111; 
Chen, X.M., Jin, Z.M., Fisher, J., (2008) Proc. Inst. Mech. Eng. Part J. Eng. Tribol., 222 (3), p. 513; 
Kumar, P., Oka, M., Ikeuchi, K., Shimizu, K., Yamamuro, T., Okumura, H., Kotoura, Y., (1991) J. Biomater. Mater. Res., 25, p. 813; 
Roba, M., Naka, M., Gautier, E., Spencer, N.D., Crockett, R., (2009) Biomaterials, 30, p. 2072; 
Karuppiah, K.S.K., Sundararajan, S., Xu, Z.-H., Li, X., (2006) Tribol. Lett., 22 (2), p. 181; 
Yang, C.-B., Fang, H.-W., Liu, H.-L., Chang, C.-H., Hsieh, M.-C., Lee, W.-M., Huang, H.-T., (2006) Chem. Phys. Lett., 431, p. 380; 
Fang, H.-W., Wang, H.-C., Tsai, T.-H., Tsai, W.-B., Hou, S.-Y., Liu, H.-L., Lee, W.-H., Huang, C.-H., (2008) J. Appl. Polym. Sci., 108, p. 2428; 
Zappone, B., Rosenberg, K.J., Israelachvili, J., (2007) Tribol. Lett., 26 (3), p. 191; 
Grill, A., (1997) Surf. Coat. Technol., (1-3), p. 507; 
Fontaine, J., Belin, M., Le Mogne, T., Grill, A., (2004) Tribol. Int., 37, p. 869; 
Sanchez-Lopez, J.C., Erdemir, A., Donnet, C., Rojas, T.C., (2003) Surf. Coat. Technol., 163, p. 444; 
Robertson, J., (2002) Mater. Sci. Eng., 37, p. 129
},
	month = {2010///},
	pages = {3451 - 3458},
	abstract = {A commonly used strategy for reduction of wear and friction of the sliding surfaces in prosthetic pairs is the use of hard coatings. DLCs (diamond like carbons) offer a promising solution, because these coatings, already used in other biomedical applications, exhibit a unique combination of chemical inertness, mechanical and tribological properties. In the present work we investigated the role of albumin, the most abundant protein in the periprosthetic fluid, on the tribological behavior of two DLCs with different compositions, against ultra-high molecular weight polyethylene (UHMWPE). The results are compared with those obtained with uncoated stainless steel surfaces. We have characterized the coatings by atomic force microscopy, X-ray diffraction, Raman spectroscopy, ultramicrohardness and wettability measurements. The adsorption of albumin onto the surfaces was investigated by ellipsometry and using a quartz crystal microbalance. Both DLCs adsorb a larger amount of protein than stainless steel. The tribological tests were performed in microtribometer aiming for a deeper understanding of the tribological mechanisms which occur at the submicrometric contact scale. Results showed no significant differences between the tribological behavior of the two coatings. Under the tested conditions, the tribological performance does not improve significantly with the application of the DLCs. Moreover, the presence of albumin on the lubricant leads to higher friction of the polymer against all counterfaces. This effect was attributed to the formation of bridges between the two sliding surfaces by the denaturated protein. The significant differences in the adsorbed amounts of protein onto the stainless steel and the two types of DLCs did not affect considerably the tribological behavior of these materials. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {Adsorbed amount, Adsorption, Albumin adsorption, Atomic force microscopy, Atomic spectroscopy, Biomedical applications, Chemical inertness, Corrosion resistant alloys, Diamond like carbon, DLC coatings, Friction, Hard coatings, Joint prostheses, Mechanical and tribological properties, Mechanical properties, Microtribology, Microtribometer, Nonmetallic matrix composites, Oxide minerals, Polyethylenes, Proteins, Quartz, Raman spectroscopy, Scanning electron microscopy, Sliding surface, Stainless steel, Stainless steel surface, Steel corrosion, Surfaces, Tribological behaviors, Tribological mechanism, Tribological performance, Tribological tests, Tribology, UHMW-PE, UHMWPE, Ultramicrohardness, Wear and friction, Wettability measurements, X ray diffraction},
	isbn = {02578972 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953715334\&partnerID=40\&md5=d724bda7404d809fed9bafb6a5a337e3},
	author = {Carapeto, A.P. and Serro, A.P. and Nunes, B.M.F. and Martins, M.C.L. and Todorovic, S. and Duarte, M.T. and Andr{\'e}, V. and Cola{\c c}o, R. and Saramago, B.}
}
@article {2258,
	title = {Neuropeptide y and osteoblast differentiation - The balance between the neuro-osteogenic network and local control: Review Article},
	journal = {FEBS JournalFEBS J.},
	volume = {277},
	year = {2010},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: Scopusdoi: 10.1111/j.1742-4658.2010.07774.xPubMed ID: 20681986Language of Original Document: EnglishCorrespondence Address: Sousa, M. M.; IBMC, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: msousa@ibmc.up.ptChemicals/CAS: neuropeptide Y, 82785-45-3, 83589-17-7; Leptin; Neuropeptide Y; Protein Isoforms; Receptors, Neuropeptide YReferences: Bellido, T., Ali, A.A., Gubrij, I., Plotkin, L.I., Fu, Q., O{\textquoteright}Brien, C.A., Manolagas, S.C., Jilka, R.L., Chronic elevation of parathyroid hormone in mice reduces expression of sclerostin by osteocytes: A novel mechanism for hormonal control of osteoblastogenesis (2005) Endocrinology, 146, pp. 4577-4583;Keller, H., Kneissel, M., SOST is a target gene for PTH in bone (2005) Bone, 37, pp. 148-158; 
O{\textquoteright}Brien, C.A., Plotkin, L.I., Galli, C., Goellner, J.J., Gortazar, A.R., Allen, M.R., Robling, A.G., Turner, C.H., Control of bone mass and remodeling by PTH receptor signaling in osteocytes (2008) PLoS ONE, 3, p. 2942; 
Robling, A.G., Niziolek, P.J., Baldridge, L.A., Condon, K.W., Allen, M.R., Alam, I., Mantila, S.M., Harris, S.E., Mechanical stimulation of bone in vivo reduces osteocyte expression of Sost/sclerostin (2008) J Biol Chem, 283, pp. 5866-5875; 
Tatsumi, S., Ishii, K., Amizuka, N., Li, M., Kobayashi, T., Kohno, K., Ito, M., Ikeda, K., Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction (2007) Cell Metab, 5, pp. 464-475; 
McDonald, A.C., Schuijers, J.A., Shen, P.J., Gundlach, A.L., Grills, B.L., Expression of galanin and galanin receptor-1 in normal bone and during fracture repair in the rat (2003) Bone, 33, pp. 788-797; 
Pacifici, R., Cytokines, estrogen, and postmenopausal osteoporosis - The second decade (1998) Endocrinology, 139, pp. 2659-2661; 
You, L., Temiyasathit, S., Lee, P., Kim, C.H., Tummala, P., Yao, W., Kingery, W., Jacobs, C.R., Osteocytes as mechanosensors in the inhibition of bone resorption due to mechanical loading (2008) Bone, 42, pp. 172-179; 
Lerner, U.H., The role of skeletal nerve fibers in bone metabolism (2000) Endocrinologist, 10, pp. 377-382; 
Sherman, M.S., The nerves of bone (1963) J Bone Joint Surg Am, 45, pp. 522-528; 
Kuntz, A., Richins, C.A., Innervation of the bone marrow (1945) J Comp Neurol, 83, pp. 213-222; 
Miller, M.R., Kasahara, M., Observations on innervation of human long bones (1963) Anat Rec, 145, pp. 13-23; 
Thurston, T.J., Distribution of nerves in long bones as shown by silver impregnation (1982) J Anat, 134, pp. 719-728; 
Calvo, W., Fortezav, J., On development of bone marrow innervation in new-born rats as studied with silver impregnation and electron microscopy (1969) Am J Anat, 126, pp. 355-371; 
Sisask, G., Bjurholm, A., Ahmed, M., Kreicbergs, A., The development of autonomic innervation in bone and joints of the rat (1996) J Auton Nerv Syst, 59, pp. 27-33; 
Gajda, M., Litwin, J.A., Cichocki, T., Timmermans, J.P., Adriaensen, D., Development of sensory innervation in rat tibia: Co-localization of CGRP and substance P with growth-associated protein 43 (GAP-43) (2005) J Anat, 207, pp. 135-144; 
Jackman, A., Fitzgerald, M., Development of peripheral hindlimb and central spinal cord innervation by subpopulations of dorsal root ganglion cells in the embryonic rat (2000) J Comp Neurol, 418, pp. 281-298; 
Kosaras, B., Jakubowski, M., Kainz, V., Burstein, R., Sensory innervation of the calvarial bones of the mouse (2009) J Comp Neurol, 515, pp. 331-348; 
Jones, K.B., Mollano, A.V., Morcuende, J.A., Cooper, R.R., Saltzman, C.L., Bone and brain: A review of neural, hormonal, and musculoskeletal connections (2004) Iowa Orthop J, 24, pp. 123-132; 
Alberius, P., Skagerberg, G., Adrenergic innervation of the calvarium of the neonatal rat. Its relationship to the sagittal suture and developing parietal bones (1990) Anat Embryol (Berl), 182, pp. 493-498; 
Herskovits, M.S., Hallas, B.H., Singh, I.J., Study of sympathetic innervation of cranial bones by axonal transport of horseradish peroxidase in the rat: Preliminary findings (1993) Acta Anat (Basel), 147, pp. 178-183; 
Hill, E.L., Elde, R., Distribution of CGRP-, VIP-, D beta H-, SP-, and NPY-immunoreactive nerves in the periosteum of the rat (1991) Cell Tissue Res, 264, pp. 469-480; 
Kruger, L., Silverman, J.D., Mantyh, P.W., Sternini, C., Brecha, N.C., Peripheral patterns of calcitonin-gene-related peptide general somatic sensory innervation: Cutaneous and deep terminations (1989) J Comp Neurol, 280, pp. 291-302; 
Silverman, J.D., Kruger, L., Calcitonin-gene-related-peptide-immunoreactive innervation of the rat head with emphasis on specialized sensory structures (1989) J Comp Neurol, 280, pp. 303-330; 
Ducy, P., Amling, M., Takeda, S., Priemel, M., Schilling, A.F., Beil, F.T., Shen, J., Karsenty, G., Leptin inhibits bone formation through a hypothalamic relay: A central control of bone mass (2000) Cell, 100, pp. 197-207; 
Robertson, S.A., Leinninger, G.M., Myers, Jr.M.G., Molecular and neural mediators of leptin action (2008) Physiol Behav, 94, pp. 637-642; 
Ahima, R.S., Flier, J.S., Leptin (2000) Annu Rev Physiol, 62, pp. 413-437; 
Tartaglia, L.A., Dembski, M., Weng, X., Deng, N., Culpepper, J., Devos, R., Richards, G.J., Deeds, J., Identification and expression cloning of a leptin receptor, OB-R (1995) Cell, 83, pp. 1263-1271; 
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., Friedman, J.M., Positional cloning of the mouse obese gene and its human homologue (1994) Nature, 372, pp. 425-432; 
Takeda, S., Elefteriou, F., Levasseur, R., Liu, X., Zhao, L., Parker, K.L., Armstrong, D., Karsenty, G., Leptin regulates bone formation via the sympathetic nervous system (2002) Cell, 111, pp. 305-317; 
Stephens, T.W., Basinski, M., Bristow, P.K., Bue-Valleskey, J.M., Burgett, S.G., Craft, L., Hale, J., Kriauciunas, A., The role of neuropeptide y in the antiobesity action of the obese gene product (1995) Nature, 377, pp. 530-532; 
Chronwall, B.M., Dimaggio, D.A., Massari, V.J., Pickel, V.M., Ruggiero, D.A., O{\textquoteright}Donohue, T.L., The anatomy of neuropeptide-Y-containing neurons in rat brain (1985) Neuroscience, 15, pp. 1159-1181; 
Tatemoto, K., Carlquist, M., Mutt, V., Neuropeptide y - A novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide (1982) Nature, 296, pp. 659-660; 
Pedrazzini, T., Pralong, F., Grouzmann, E., Neuropeptide Y: The universal soldier (2003) Cell Mol Life Sci, 60, pp. 350-377; 
Erickson, J.C., Hollopeter, G., Palmiter, R.D., Attenuation of the obesity syndrome of ob/ob mice by the loss of neuropeptide y (1996) Science, 274, pp. 1704-1707; 
Allison, S.J., Baldock, P.A., Herzog, H., The control of bone remodeling by neuropeptide y receptors (2007) Peptides, 28, pp. 320-325; 
Bjurholm, A., Kreicbergs, A., Terenius, L., Goldstein, M., Schultzberg, M., Neuropeptide Y-, tyrosine hydroxylase- and vasoactive intestinal polypeptide-immunoreactive nerves in bone and surrounding tissues (1988) J Auton Nerv Syst, 25, pp. 119-125; 
Ahmed, M., Bjurholm, A., Kreicbergs, A., Schultzberg, M., Neuropeptide Y, tyrosine hydroxylase and vasoactive intestinal polypeptide-immunoreactive nerve fibers in the vertebral bodies, discs, dura mater, and spinal ligaments of the rat lumbar spine (1993) Spine, 18, pp. 268-273; 
Hill, E.L., Turner, R., Elde, R., Effects of neonatal sympathectomy and capsaicin treatment on bone remodeling in rats (1991) Neuroscience, 44, pp. 747-755; 
Lindblad, B.E., Nielsen, L.B., Jespersen, S.M., Bjurholm, A., Bunger, C., Hansen, E.S., Vasoconstrictive action of neuropeptide y in bone. The porcine tibia perfused in vivo (1994) Acta Orthop Scand, 65, pp. 629-634; 
Ahmad, T., Ugarph-Morawski, A., Li, J., Bileviciute-Ljungar, I., Finn, A., Ostenson, C.G., Kreicbergs, A., Bone and joint neuropathy in rats with type-2 diabetes (2004) Regul Pept, 119, pp. 61-67; 
Ericsson, A., Schalling, M., McIntyre, K.R., Lundberg, J.M., Larhammar, D., Seroogy, K., Hokfelt, T., Persson, H., Detection of neuropeptide y and its mRNA in megakaryocytes: Enhanced levels in certain autoimmune mice (1987) Proc Natl Acad Sci USA, 84, pp. 5585-5589; 
Ahmed, M., Srinivasan, G.R., Theodorsson, E., Bjurholm, A., Kreicbergs, A., Extraction and quantitation of neuropeptides in bone by radioimmunoassay (1994) Regul Pept, 51, pp. 179-188; 
Bjurholm, A., Neuroendocrine peptides in bone (1991) Int Orthop, 15, pp. 325-329; 
Bjurholm, A., Kreicbergs, A., Schultzberg, M., Lerner, U.H., Neuroendocrine regulation of cyclic AMP formation in osteoblastic cell lines (UMR-106-01, ROS 17/2.8, MC3T3-E1, and Saos-2) and primary bone cells (1992) J Bone Miner Res, 7, pp. 1011-1019; 
Lundberg, P., Allison, S.J., Lee, N.J., Baldock, P.A., Brouard, N., Rost, S., Enriquez, R.F., Simmons, P., Greater bone formation of Y2 knockout mice is associated with increased osteoprogenitor numbers and altered Y1 receptor expression (2007) J Biol Chem, 282, pp. 19082-19091; 
Nakamura, M., Sakanaka, C., Aoki, Y., Ogasawara, H., Tsuji, T., Kodama, H., Matsumoto, T., Noma, M., Identification of two isoforms of mouse neuropeptide Y-Y1 receptor generated by alternative splicing. Isolation, genomic structure, and functional expression of the receptors (1995) J Biol Chem, 270, pp. 30102-30110; 
Togari, A., Arai, M., Mizutani, S., Koshihara, Y., Nagatsu, T., Expression of mRNAs for neuropeptide receptors and beta-adrenergic receptors in human osteoblasts and human osteogenic sarcoma cells (1997) Neurosci Lett, 233, pp. 125-128; 
Igwe, J.C., Jiang, X., Paic, F., Ma, L., Adams, D.J., Baldock, P.A., Pilbeam, C.C., Kalajzic, I., Neuropeptide y is expressed by osteocytes and can inhibit osteoblastic activity (2009) J Cell Biochem, 108, pp. 621-630; 
Nunes, A.F., Liz, M.A., Franquinho, F., Teixeira, L., Sousa, V., Chenu, C., Lamghari, M., Sousa, M.M., Neuropeptide y expression and function during osteoblast differentiation - Insights from transthyretin knockout mice (2010) FEBS J, 277, pp. 263-275; 
Baldock, P.A., Sainsbury, A., Couzens, M., Enriquez, R.F., Thomas, G.P., Gardiner, E.M., Herzog, H., Hypothalamic Y2 receptors regulate bone formation (2002) J Clin Invest, 109, pp. 915-921; 
Ducy, P., Schinke, T., Karsenty, G., The osteoblast: A sophisticated fibroblast under central surveillance (2000) Science, 289, pp. 1501-1504; 
Allison, S.J., Baldock, P., Sainsbury, A., Enriquez, R., Lee, N.J., Lin, E.J., Klugmann, M., Li, M., Conditional deletion of hypothalamic Y2 receptors reverts gonadectomy-induced bone loss in adult mice (2006) J Biol Chem, 281, pp. 23436-23444; 
Clegg, D.J., Brown, L.M., Zigman, J.M., Kemp, C.J., Strader, A.D., Benoit, S.C., Woods, S.C., Geary, N., Estradiol-dependent decrease in the orexigenic potency of ghrelin in female rats (2007) Diabetes, 56, pp. 1051-1058; 
Zengin, A., Zhang, L., Herzog, H., Baldock, P.A., Sainsbury, A., Neuropeptide y and sex hormone interactions in humoral and neuronal regulation of bone and fat (2010) Trends Endocrinol Metab, 21, pp. 411-418; 
Hosaka, H., Nagata, A., Yoshida, T., Shibata, T., Nagao, T., Tanaka, T., Saito, Y., Tatsuno, I., Pancreatic polypeptide is secreted from and controls differentiation through its specific receptors in osteoblastic MC3T3-E1 cells (2008) Peptides, 29, pp. 1390-1395; 
Teixeira, L., Sousa, D.M., Nunes, A.F., Sousa, M.M., Herzog, H., Lamghari, M., NPY revealed as a critical modulator of osteoblast function in vitro: New insights into the role of Y1 and Y2 receptors (2009) J Cell Biochem, 107, pp. 908-916; 
Sainsbury, A., Schwarzer, C., Couzens, M., Herzog, H., Y2 receptor deletion attenuates the type 2 diabetic syndrome of ob/ob mice (2002) Diabetes, 51, pp. 3420-3427; 
Wilding, J.P., Gilbey, S.G., Bailey, C.J., Batt, R.A., Williams, G., Ghatei, M.A., Bloom, S.R., Increased neuropeptide-Y messenger ribonucleic acid (mRNA) and decreased neurotensin mRNA in the hypothalamus of the obese (ob/ob) mouse (1993) Endocrinology, 132, pp. 1939-1944; 
Baldock, P.A., Allison, S., McDonald, M.M., Sainsbury, A., Enriquez, R.F., Little, D.G., Eisman, J.A., Herzog, H., Hypothalamic regulation of cortical bone mass: Opposing activity of Y2 receptor and leptin pathways (2006) J Bone Miner Res, 21, pp. 1600-1607; 
Baldock, P.A., Sainsbury, A., Allison, S., Lin, E.J., Couzens, M., Boey, D., Enriquez, R., Gardiner, E.M., Hypothalamic control of bone formation: Distinct actions of leptin and y2 receptor pathways (2005) J Bone Miner Res, 20, pp. 1851-1857; 
Baldock, P.A., Allison, S.J., Lundberg, P., Lee, N.J., Slack, K., Lin, E.J., Enriquez, R.F., During, M.J., Novel role of Y1 receptors in the coordinated regulation of bone and energy homeostasis (2007) J Biol Chem, 282, pp. 19092-19102; 
Lee, N.J., Doyle, K.L., Sainsbury, A., Enriquez, R.F., Hort, Y.J., Riepler, S.J., Baldock, P.A., Herzog, H., Critical role for Y1 receptors in mesenchymal progenitor cell differentiation and osteoblast activity (2010) J Bone Miner Res, , doi:; 
Allison, S.J., Baldock, P.A., Enriquez, R.F., Lin, E., During, M., Gardiner, E.M., Eisman, J.A., Herzog, H., Critical interplay between neuropeptide y and sex steroid pathways in bone and adipose tissue homeostasis (2009) J Bone Miner Res, 24, pp. 294-304; 
Sainsbury, A., Baldock, P.A., Schwarzer, C., Ueno, N., Enriquez, R.F., Couzens, M., Inui, A., Gardiner, E.M., Synergistic effects of Y2 and Y4 receptors on adiposity and bone mass revealed in double knockout mice (2003) Mol Cell Biol, 23, pp. 5225-5233; 
Elefteriou, F., Takeda, S., Liu, X., Armstrong, D., Karsenty, G., Monosodium glutamate-sensitive hypothalamic neurons contribute to the control of bone mass (2003) Endocrinology, 144, pp. 3842-3847; 
Baldock, P.A., Lee, N.J., Driessler, F., Lin, S., Allison, S., Stehrer, B., Lin, E.J., Wong, I.P., Neuropeptide y knockout mice reveal a central role of NPY in the coordination of bone mass to body weight (2009) PLoS ONE, 4, p. 8415; 
Sainsbury, A., Cusin, I., Doyle, P., Rohner-Jeanrenaud, F., Jeanrenaud, B., Intracerebroventricular administration of neuropeptide y to normal rats increases obese gene expression in white adipose tissue (1996) Diabetologia, 39, pp. 353-356; 
Sainsbury, A., Herzog, H., Inhibitory effects of central neuropeptide y on the somatotropic and gonadotropic axes in male rats are independent of adrenal hormones (2001) Peptides, 22, pp. 467-471; 
Nunes, A.F., Saraiva, M.J., Sousa, M.M., Transthyretin knockouts are a new mouse model for increased neuropeptide y (2006) FASEB J, 20, pp. 166-168; 
Prigge, S.T., Mains, R.E., Eipper, B.A., Amzel, L.M., New insights into copper monooxygenases and peptide amidation: Structure, mechanism and function (2000) Cell Mol Life Sci, 57, pp. 1236-1259; 
Eipper, B.A., Stoffers, D.A., Mains, R.E., The biosynthesis of neuropeptides: Peptide alpha-amidation (1992) Annu Rev Neurosci, 15, pp. 57-85; 
Marques, F., Sousa, J.C., Oliveira, P., Oliveira, H.C., Palha, J.A., The absence of transthyretin does not impair regulation of lipid and glucose metabolism (2007) Horm Metab Res, 39, pp. 529-533
},
	month = {2010///},
	pages = {3664 - 3674},
	abstract = {Accumulating evidence has contributed to a novel view in bone biology: bone remodeling, specifically osteoblast differentiation, is under the tight control of the central and peripheral nervous systems. Among other players in this neuro-osteogenic network, the neuropeptide Y (NPY) system has attracted particular attention. At the central nervous system level, NPY exerts its function in bone homeostasis through the hypothalamic Y2 receptor. Locally in the bone, NPY action is mediated by its Y1 receptor. Besides the presence of Y1, a complex network exists locally: not only there is input of the peripheral nervous system, as the bone is directly innervated by NPY-containing fibers, but there is also input from non-neuronal cells, including bone cells capable of NPY expression. The interaction of these distinct players to achieve a multilevel control system of bone homeostasis is still under debate. In this review, we will integrate the current knowledge on the impact of the NPY system in bone biology, and discuss the mechanisms through which the balance between central and the peripheral NPY action might be achieved. {\textcopyright} 2010 FEBS.},
	keywords = {Animals, Bone and Bones, bone cell, bone innervation, bone mass, cell differentiation, Homeostasis, Humans, immunoreactivity, leptin, Nerve Net, neuropeptide Y, neuropeptide Y receptor, neuropeptide Y2 receptor, nonhuman, NPY, NPY receptors, osteoblast, Osteoblasts, phenotype, priority journal, protein expression, protein interaction, Protein Isoforms, Receptors, Neuropeptide Y, review},
	isbn = {1742464X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956337408\&partnerID=40\&md5=2d7cd3f7e81e95ea895958fff6902b28},
	author = {Franquinho, F. and Liz, M.A. and Nunes, A.F. and Neto, E. and Lamghari, M. and Sousa, M.M.}
}
@article {2289,
	title = {Neuropeptide y expression and function during osteoblast differentiation - Insights from transthyretin knockout mice},
	journal = {FEBS JournalFEBS J.},
	volume = {277},
	year = {2010},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: Scopusdoi: 10.1111/j.1742-4658.2009.07482.xPubMed ID: 19954489Language of Original Document: EnglishCorrespondence Address: Sousa, M. M.; IBMC, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: msousa@ibmc.up.ptChemicals/CAS: neuropeptide Y, 82785-45-3, 83589-17-7; Amides; DNA Primers; Neuropeptide Y; Prealbumin; RNA, MessengerReferences: McDonald, A.C., Schuijers, J.A., Shen, P.J., Gundlach, A.L., Grills, B.L., Expression of galanin and galanin receptor-1 in normal bone and during fracture repair in the rat (2003) Bone, 33, pp. 788-797;Pacifici, R., Cytokines, estrogen, and postmenopausal osteoporosis - The second decade (1998) Endocrinology, 139, pp. 2659-2661; 
You, L., Temiyasathit, S., Lee, P., Kim, C.H., Tummala, P., Yao, W., Kingery, W., Jacobs, C.R., Osteocytes as mechanosensors in the inhibition of bone resorption due to mechanical loading (2008) Bone, 42, pp. 172-179; 
Chenu, C., Marenzana, M., Sympathetic nervous system and bone remodeling (2005) Joint Bone Spine, 72 (6), pp. 481-483. , DOI 10.1016/j.jbspin.2005.10.007, PII S1297319X05001661; 
Baldock, P.A., Sainsbury, A., Couzens, M., Enriquez, R.F., Thomas, G.P., Gardiner, E.M., Herzog, H., Hypothalamic Y2 receptors regulate bone formation (2002) J Clin Invest, 109, pp. 915-921; 
Baldock, P.A., Allison, S.J., Lundberg, P., Lee, N.J., Slack, K., Lin, E.J., Enriquez, R.F., During, M.J., Novel role of Y1 receptors in the coordinated regulation of bone and energy homeostasis (2007) J Biol Chem, 282, pp. 19092-19102; 
Ducy, P., Amling, M., Takeda, S., Priemel, M., Schilling, A.F., Beil, F.T., Shen, J., Karsenty, G., Leptin inhibits bone formation through a hypothalamic relay: A central control of bone mass (2000) Cell, 100, pp. 197-207; 
Elefteriou, F., Takeda, S., Liu, X., Armstrong, D., Karsenty, G., Monosodium glutamate-sensitive hypothalamic neurons contribute to the control of bone mass (2003) Endocrinology, 144, pp. 3842-3847; 
Elefteriou, F., Neuronal signaling and the regulation of bone remodeling (2005) Cell Mol Life Sci, 62, pp. 2339-2349; 
Bjurholm, A., Kreicbergs, A., Terenius, L., Goldstein, M., Schultzberg, M., Neuropeptide Y-, tyrosine hydroxylase- and vasoactive intestinal polypeptide-immunoreactive nerves in bone and surrounding tissues (1988) J Auton Nerv Syst, 25, pp. 119-125; 
Hill, E.L., Elde, R., Distribution of CGRP-, VIP-, D beta H-, SP-, and NPY-immunoreactive nerves in the periosteum of the rat (1991) Cell Tissue Res, 264, pp. 469-480; 
Tabarowski, Z., Gibson-Berry, K., Felten, S.Y., Noradrenergic and peptidergic innervation of the mouse femur bone marrow (1996) Acta Histochem, 98, pp. 453-457; 
Ahmed, M., Srinivasan, G.R., Theodorsson, E., Bjurholm, A., Kreicbergs, A., Extraction and quantitation of neuropeptides in bone by radioimmunoassay (1994) Regul Pept, 51, pp. 179-188; 
Ericsson, A., Schalling, M., McIntyre, K.R., Lundberg, J.M., Larhammar, D., Seroogy, K., Hokfelt, T., Persson, H., Detection of neuropeptide y and its mRNA in megakaryocytes: Enhanced levels in certain autoimmune mice (1987) Proc Natl Acad Sci USA, 84, pp. 5585-5589; 
Lundberg, P., Allison, S.J., Lee, N.J., Baldock, P.A., Brouard, N., Rost, S., Enriquez, R.F., Simmons, P., Greater bone formation of y2 knockout mice is associated with increased osteoprogenitor numbers and altered y1 receptor expression (2007) J Biol Chem, 282, pp. 19082-19091; 
Stephens, T.W., Basinski, M., Bristow, P.K., Bue-Valleskey, J.M., Burgett, S.G., Craft, L., Hale, J., Kriauciunas, A., The role of neuropeptide y in the antiobesity action of the obese gene product (1995) Nature, 377, pp. 530-532; 
Baldock, P.A., Sainsbury, A., Allison, S., Lin, E.J., Couzens, M., Boey, D., Enriquez, R., Gardiner, E.M., Hypothalamic control of bone formation: Distinct actions of leptin and y2 receptor pathways (2005) J Bone Miner Res, 20, pp. 1851-1857; 
Allison, S., Baldock, P., Enriquez, R., Lin, E., During, M., Gardiner, E., Eisman, J., Herzog, H., Critical interplay between neuropeptide y and sex steroid pathways in bone and adipose tissue homeostasis (2008) J Bone Miner Res, 24, pp. 294-304; 
Nunes, A.F., Saraiva, M.J., Sousa, M.M., Transthyretin knockouts are a new mouse model for increased neuropeptide y (2006) FASEB J, 20, pp. 166-168; 
Mains, R.E., Cullen, E.I., May, V., Eipper, B.A., The role of secretory granules in peptide biosynthesis (1987) Ann NY Acad Sci, 493, pp. 278-291; 
Prigge, S.T., Mains, R.E., Eipper, B.A., Amzel, L.M., New insights into copper monooxygenases and peptide amidation: Structure, mechanism and function (2000) Cell Mol Life Sci, 57, pp. 1236-1259; 
Eipper, B.A., Stoffers, D.A., Mains, R.E., The biosynthesis of neuropeptides: Peptide alpha-amidation (1992) Annu Rev Neurosci, 15, pp. 57-85; 
Teixeira, L., Sousa, D.M., Nunes, A.F., Sousa, M.M., Herzog, H., Lamghari, M., NPY revealed as a critical modulator of osteoblast function in vitro: New insights into the role of Y1 and Y2 receptors (2009) J Cell Biochem, 107, pp. 908-916; 
Amano, S., Arai, M., Goto, S., Togari, A., Inhibitory effect of NPY on isoprenaline-induced osteoclastogenesis in mouse bone marrow cells (2007) Biochim Biophys Acta, 1770, pp. 966-973; 
Baldock, P.A., Allison, S., McDonald, M.M., Sainsbury, A., Enriquez, R.F., Little, D.G., Eisman, J.A., Herzog, H., Hypothalamic regulation of cortical bone mass: Opposing activity of Y2 receptor and leptin pathways (2006) J Bone Miner Res, 21, pp. 1600-1607; 
Saper, C.B., Loewy, A.D., Swanson, L.W., Cowan, W.M., Direct hypothalamo-autonomic connections (1976) Brain Res, 117, pp. 305-312; 
Navia, J.M., Harris, S.S., Vitamin A influence on calcium metabolism and calcification (1980) Ann NY Acad Sci, 355, pp. 45-57; 
Wang, A., Ding, X., Sheng, S., Yao, Z., Retinoic acid inhibits osteogenic differentiation of rat bone marrow stromal cells (2008) Biochem Biophys Res Commun, 375, pp. 435-439; 
Takahashi, T., Kamiya, N., Kawabata, N., Takagi, M., The effect of retinoic acid on a zinc finger transcription factor, AJ18, during differentiation of a rat clonal preosteoblastic cell line, ROB-C20, into osteoblasts (2008) Arch Oral Biol, 53, pp. 87-94; 
Grouzmann, E., Comoy, E., Walker, P., Burnier, M., Bohuon, C., Waeber, B., Brunner, H., Production and characterization of four anti-neuropeptide y monoclonal antibodies (1992) Hybridoma, 11, pp. 409-424; 
Wei, S., Episkopou, V., Piantedosi, R., Maeda, S., Shimada, K., Gottesman, M.E., Blaner, W.S., Studies on the metabolism of retinol and retinol-binding protein in transthyretin-deficient mice produced by homologous recombination (1995) J Biol Chem, 270, pp. 866-870; 
Karga, H., Papapetrou, P.D., Korakovouni, A., Papandroulaki, F., Polymeris, A., Pampouras, G., Bone mineral density in hyperthyroidism (2004) Clin Endocrinol, 61, pp. 466-472; 
Episkopou, V., Maeda, S., Nishiguchi, S., Shimada, K., Gaitanaris, G.A., Gottesman, M.E., Robertson, E.J., Disruption of the transthyretin gene results in mice with depressed levels of plasma retinol and thyroid hormone (1993) Proc Natl Acad Sci USA, 90, pp. 2375-2379; 
Palha, J.A., Episkopou, V., Maeda, S., Shimada, K., Gottesman, M.E., Saraiva, M.J., Thyroid hormone metabolism in a transthyretin-null mouse strain (1994) J Biol Chem, 269, pp. 33135-33139; 
Palha, J.A., Hays, M.T., Morreale De Escobar, G., Episkopou, V., Gottesman, M.E., Saraiva, M.J., Transthyretin is not essential for thyroxine to reach the brain and other tissues in transthyretin-null mice (1997) Am J Physiol, 272, pp. 485-E493; 
Palha, J.A., Fernandes, R., De Escobar, G.M., Episkopou, V., Gottesman, M., Saraiva, M.J., Transthyretin regulates thyroid hormone levels in the choroid plexus, but not in the brain parenchyma: Study in a transthyretin-null mouse model (2000) Endocrinology, 141, pp. 3267-3272; 
Kingery, W.S., Offley, S.C., Guo, T.Z., Davies, M.F., Clark, J.D., Jacobs, C.R., A substance P receptor (NK1) antagonist enhances the widespread osteoporotic effects of sciatic nerve section (2003) Bone, 33, pp. 927-936; 
Hosaka, H., Nagata, A., Yoshida, T., Shibata, T., Nagao, T., Tanaka, T., Saito, Y., Tatsuno, I., Pancreatic polypeptide is secreted from and controls differentiation through its specific receptors in osteoblastic MC3T3-E1 cells (2008) Peptides, 29, pp. 1390-1395; 
Fleming, C.E., Saraiva, M.J., Sousa, M.M., Transthyretin enhances nerve regeneration (2007) J Neurochem, 103, pp. 831-839; 
Sainsbury, A., Cusin, I., Doyle, P., Rohner-Jeanrenaud, F., Jeanrenaud, B., Intracerebroventricular administration of neuropeptide y to normal rats increases obese gene expression in white adipose tissue (1996) Diabetologia, 39, pp. 353-356; 
Sainsbury, A., Herzog, H., Inhibitory effects of central neuropeptide y on the somatotropic and gonadotropic axes in male rats are independent of adrenal hormones (2001) Peptides, 22, pp. 467-471; 
Marques, F., Sousa, J.C., Oliveira, P., Oliveira, H.C., Palha, J.A., The absence of transthyretin does not impair regulation of lipid and glucose metabolism (2007) Horm Metab Res, 39, pp. 529-533; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Salingcarnboriboon, R., Tsuji, K., Komori, T., Nakashima, K., Ezura, Y., Noda, M., Runx2 is a target of mechanical unloading to alter osteoblastic activity and bone formation in vivo (2006) Endocrinology, 147, pp. 2296-2305
},
	month = {2010///},
	pages = {263 - 275},
	abstract = {To better understand the role of neuropeptide Y (NPY) in bone homeostasis, as its function in the regulation of bone mass is unclear, we assessed its expression in this tissue. By immunohistochemistry, we demonstrated, both at embryonic stages and in the adult, that NPY is synthesized by osteoblasts, osteocytes, and chondrocytes. Moreover, peptidylglycine α-amidating monooxygenase, the enzyme responsible for NPY activation by amidation, was also expressed in these cell types. Using transthyretin (TTR) KO mice as a model of augmented NPY levels, we showed that this strain has increased NPY content in the bone, further validating the expression of this neuropeptide by bone cells. Moreover, the higher amidated neuropeptide levels in TTR KO mice were related to increased bone mineral density and trabecular volume. Additionally, RT-PCR analysis established that NPY is not only expressed in MC3T3-E1 osteoblastic cells and bone marrow stromal cells (BMSCs), but is also detectable by RIA in BMSCs undergoing osteoblastic differentiation. In agreement with our in vivo observations, in vitro, TTR KO BMSCs differentiated in osteoblasts had increased NPY levels and exhibited enhanced competence in undergoing osteoblastic differentiation. In summary, this work contributes to a better understanding of the role of NPY in the regulation of bone formation by showing that this neuropeptide is expressed in bone cells and that increased amidated neuropeptide content is related to increased bone mass. {\textcopyright} 2009 FEBS.},
	keywords = {3T3 Cells, alpha amidating enzyme, Amidated neuropeptide, amidation, Amides, animal cell, animal tissue, Animals, article, Base Sequence, Bone Density, bone development, bone marrow cell, Bone Marrow Cells, Bone marrow stromal cells, bone mass, cartilage cell, cell differentiation, Cells, Cultured, Chondrocytes, controlled study, developmental stage, DNA Primers, embryo, embryo development, Homeostasis, immunohistochemistry, male, Mice, Mice, Knockout, mouse, Mus, neuropeptide Y, nonhuman, NPY, ossification, osteoblast, Osteoblastic differentiation, Osteoblasts, osteocyte, Osteocytes, peptide synthesis, prealbumin, priority journal, protein expression, protein function, radioimmunoassay, regulatory mechanism, reverse transcription polymerase chain reaction, RNA, Messenger, stroma cell, Stromal Cells, trabecular bone},
	isbn = {1742464X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-73649127668\&partnerID=40\&md5=c25c87a8e1206fc9d2b878f9611581dd},
	author = {Nunes, A.F. and Liz, M.A. and Franquinho, F. and Teixeira, L. and Sousa, V. and Chenu, C. and Lamghari, M. and Sousa, M.M.}
}
@article {2261,
	title = {Plasma surface modification of polycarbonate and poly(propylene) substrates for biomedical electrodes},
	journal = {Plasma Processes and PolymersPlasma Processes Polym.},
	volume = {7},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: Scopusdoi: 10.1002/ppap.200900176Language of Original Document: EnglishCorrespondence Address: Vaz, F.; Universidade do Minho, Centro de Fisica, Campus de Azur{\'e}m, 4800-058 Guimar{\~a}es, Portugal; email: fvaz@fisica.uminho.ptReferences: Moon, M.-W., Vaziri, A., (2009) Scripta Mater., 60, p. 44;Pandiyaraj, K., Selvarajan, V., Deshmukh, R.R., Gao, C., (2009) Appl. Surf. Sci., 255, p. 3965; 
Pandiyaraj, K., Selvarajan, V., Deshmukh, R.R., Gao, C., (2009) Vacuum, 83, p. 332; 
Tavares, C.J., Marques, S.M., Rebouta, L., Lanceros-M{\'e}ndez, S., Sencadas, V., Costa, C.M., Alves, E., Fernandes, A.J., (2008) Thin Solid Films, 517, p. 1161; 
Eom, J.S., Kim, S.H., (2008) Thin Solid Films, 516, p. 4530; 
Di Mundo, R., De Benedictis, V., Palumbo, F., D{\textquoteright}Agostino, R., (2009) Appl. Surf. Sci., 255, p. 5461; 
{\"O}zenbas, M., Tan, C., (1986) J. Cryst Growth, 78, p. 519; 
Goddard, J.M., Hotchkiss, J.H., (2007) Prog. Potym. Sci., 32, p. 698; 
Nathawat, R., Kumar, A., Kulshrestha, V., Vijay, Y.K., Kobayashi, T., Kanjilal, D., (2008) Nuel. Instrum. Methods Phys. Res. B, 266, p. 4749; 
Shin, Y.N., Kim, B.S., Ahn, H.H., Lee, J.H., Kim, K.S., Lee, J.Y., Kim, M.S., Lee, H.B., (2008) Appl. Surf. Sci., 255, p. 293; 
Lisboa, P., Gilliland, D., Ceccone, G., Valsesia, A., Rossi, F., (2006) Appl. Surf. Sci., 252, p. 4397; 
Muck, A., Svatos, A., (2007) Talanta, 74, p. 333; 
Sandoval-Paz, M.G., Ramirez-Bon, R., (2008) Thin Solid Films, 517, p. 6747; 
Park, S.K., Han, J.I., Kim, W.K., Kwak, M.G., (2001) Thin Solid Films, 397, p. 49; 
Pihan, S.A., Tsukruk, T., F{\"o}rch, R., (2009) Surf. Coat. Technol., 13, p. 1856; 
Dayss, E., Leps, G., Meinhardt, J., (1999) Surf. Coat Teehnol., 116, p. 986; 
Pandiyaraj, K.N., Selvarajan, V., Deshmukh, R.R., Bousmina, M., (2008) Surf. Coat Teehnol., 202, p. 4218; 
Cheng, C., Liye, Z., Zhan, R.-J., (2006) Surf. Coat Teehnol., 200, p. 6659; 
Lai, J., Sunderland, B., Xue, J., Yan, S., Zhao, W., Folkard, M., Michael, B.D., Wang, Y., (2006) Appl. Surf. Sci., 252, p. 3375; 
Phenton, M.J., Lovell-Hoare, M.C., Stevens, G.C., (2001) J. Phys. D: Appl. Phys., 34, p. 2754; 
Ozeki, K., Hirakuri, K.K., (2008) Appl. Surf. Sci., 254, p. 1614; 
Vallon, S., Dr{\'e}villon, B., Poncin-Epaillard, F., KlembergSapieha, J.E., Martinu, L., (1996) J. Vac. Sci. Teehnol. A, 14, p. 3194; 
Mirabedini, S.M., Arabi, H., Salem, A., Asiaban, S., (2007) Prog. Org. Coat., 60, p. 105; 
Kogelschatz, U., Elliason, B., Egli, W., (1999) Pure Appl. Chem., 71, p. 1819; 
Dorai, R., Kushner, M.J., (2003) J. Phys. D: Appl. Phys., 36, p. 666; 
Corval, G.J., Gray, D.G., Goring, D.A.I., (1976) J. Polym. Sci., Polym. Lett, 14, p. 231; 
Guimond, S., Radu, I., Czeremuszkin, G., Caisson, D.J., Wertheimer, M.R., (2002) Plasmas Polym., 7, p. 71; 
Guimond, S., Radu, I., Czeremuszkin, G., Wertheimer, M.R., (2002) Proc. 8th Int. Symp. High Pressure Low Temp. Plasma Chem., p. 400. , P{\"u}haj{\"a}rve, Estland; 
Kogelschatz, U., Elliason, B., Egli, W., (1999) Pure Appl. Chem., 71, p. 1819; 
Cho, J.-S., Kim, K.-H., Han, S., Beag, Y.-W., Koh, S.-K., (2003) Prog. Org. Coat., 48, p. 251; 
Baek, J.-Y., An, J.-H., Choi, J.-M., Park, K.-S., Lee, S.-H., (2008) Sens. Actuators A, 143, p. 423; 
Gruetzmann, A., Hansen, S., M{\"u}ller, J., (2007) Physiol Meas., 28, p. 1375; 
Ribeiro, E., Malczyk, A., Carvalho, S., Rebouta, L., Fernandes, I.V., Alves, E., Miranda, A.S., (2002) Surf. Coat. Teehnol, 151-152, p. 515; 
Sharma, R., Holcomb, E., Trigwell, S., Mazumder, M., (2007) J. Electrostat., 65, p. 269; 
Cunha, L.T., Pedrosa, P., J.Tavares, C., Alves, E., Vaz, F., Fonseca, C., (2009) Electrochim. Acta, 55, p. 59; 
M{\"u}ller, D.J.M., Janovjak, H., Lehto, T., Kuerschner, L., Anderson, K., (2002) Prog. Biophys. Mol. Biol., 79, p. 1; 
Wolansky, G., Marmur, A., (1999) Colloids Surf. A, 156, p. 381; 
Mohamed, S.H., Kappertz, O., Ngaruiya, J.M., Niemeier, T., Drese, R., Wakkad, M., Wuttig, M., (2004) Phys. Stat. Sol., 202, p. 90; 
Carrino, L., Moroni, G., Polini, W., (2002) J. Mater. Proc. Technol., 121, p. 373
},
	month = {2010///},
	pages = {676 - 686},
	abstract = {The effects of surface plasma treatments on the properties of PP and PC substrates were studied. Main results showed that the 0.48 Pa Ar treatment promoted the most favorable changes: roughness increased from 15 to 17 nm for PC and from 12 to 30 nm for PP, while the CA lowered from 95{\textdegree} to 59{\textdegree} and from 87{\textdegree} to 35{\textdegree} for PC and PP, respectively. The effects of the activation treatment on adhesion were checked by depositing a thin layer of TiN on both untreated and 0.48 Pa Ar plasma treated PC samples, and the adhesion to both substrates compared using the standard X-cut tape test. The Ar activated samples displayed better adhesion of the TiN film than the untreated ones, confirming the benefits of the surface changes induced by the plasma treatments. (Figure Presented) {\textcopyright} 2010 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {Activation treatment, Adhesion, Ar plasmas, biomedical electrodes, Deposition, Electrodes, Functional polymers, Plasma surface modifications, Plasma treatment, Plasma treatments, Plasmas, Polymers, Propylene, Surface changes, Surface modification, Surface plasma treatment, Thin layers, TiN films, Titanium nitride},
	isbn = {16128850 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956235411\&partnerID=40\&md5=e0146f15b9c6dbbc01005ef48ca837e0},
	author = {Pedrosa, P. and Chapp{\'e}, J.-M. and Fonseca, C. and Machado, A.V. and N{\'o}brega, J.M. and Vaz, F.}
}
@article {2303,
	title = {NPY revealed as a critical modulator of osteoblast function in vitro: New insights into the role of Y1 and Y2 receptors},
	journal = {Journal of Cellular BiochemistryJ. Cell. Biochem.},
	volume = {107},
	year = {2009},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: JCEBDdoi: 10.1002/jcb.22194PubMed ID: 19459152Language of Original Document: EnglishCorrespondence Address: Lamghari, M.; Instituto de Engenharia Biom{\'e}dica (INEB), Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: lamghari@ineb.up.ptChemicals/CAS: calcium, 14092-94-5, 7440-70-2; osteoclast differentiation factor, 200145-93-3; osteoprotegerin, 205944-50-9; Alkaline Phosphatase, 3.1.3.1; Neuropeptide Y; Osteoprotegerin; RANK Ligand; Receptors, Neuropeptide YReferences: Ahmed, M., Srinivasan, G.R., Theodorsson, E., Bjurholm, A., Kreicbergs, A., Extraction and quantitation of neuropeptides in bone by radioimmunoassay (1994) Regul Pept, 51, pp. 179-188;Allison, S.J., Herzog, H., NPY and bone (2006) EXS, 95, pp. 171-182; 
Allison, S.J., Baldock, P., Sainsbury, A., Enriquez, R., Lee, N.J., Lin, E.J., Klugman, M., Gardiner, E.M., Conditional deletion of hypothalamic Y2 receptors reverts gonadectomy-induced bone loss in adult mice (2006) J Biol Chem, 281, pp. 23436-23444; 
Amano, S., Arai, M., Goto, S., Togari, A., Inhibitory effect of NPY on isoprenaline-induced osteoclastogenesis in mouse bone marrow cells (2007) Biochim Biophys Acta, 1770, pp. 966-973; 
Baldock, P.A., Sainsbury, A., Couzens, M., Enriquez, R.F., Ghomas, G.P., Gardiner, E.M., Herzog, H., Hypothalamic Y2 receptors regulate bone formation (2002) J Clin Investig, 109, pp. 915-921; 
Baldock, P.A., Sainsbury, A., Allison, S., Lin, E.J., Couzens, M., Boey, D., Enriquez, R., Gardiner, E.M., Hypothalamic control of bone formation: Distinct actions of leptin and y2 receptor pathways (2005) J Bone Miner Res, 20, pp. 1851-1857; 
Baldock, P.A., Allison, S.J., Lundberg, P., Lee, N.J., Slack, K., Lin, E.J., Enriquez, R.F., Herzog, H., Novel role of Y1 receptors in the coordinated regulation of bone and energy homeostasis (2007) J Biol Chem, 282, pp. 19092-19102; 
Chenu, C., Role of innervation in the control of bone remodelling (2004) J Musculoskel Neuron Interact, 4, pp. 132-134; 
Chenu, C., Marenzana, M., Sympathetic nervous system and bone remodeling (2005) Joint Bone Spine, 72, pp. 481-483; 
Clark, J.T., Kalra, P.S., Kalra, S.P., Neuropeptide Y stimulates feeding but inhibits sexual behavior in rats (1985) Endocrinology, 117, pp. 2435-2442; 
Dumont, Y., Jacques, D., Bouchard, P., Quirion, R., Species differences in the expression and distribution of the neuropeptide Y Y1, Y2, Y4, and Y5 receptors in rodents, guinea pig, and primates brains (1998) J Comp Neurol, 402, pp. 372-384; 
Elefteriou, F., Neuronal signalling and the regulation of bone remodelling (2005) Cell Mol Life Sci, 62, pp. 2339-2349; 
Ericsson, A., Schalling, M., McIntyre, K.R., Lundberg, J.M., Larhammar, D., Seroogy, K., Hokfelt, T., Persson, H., Detection of neuropeptide Y and its mRNA in megakaryocytes: Enhanced levels in certain autoimmune mice (1987) Proc Natl Acad Sci USA, 84, pp. 5585-5589; 
Fu, Q., Manolagas, S.C., O{\textquoteright}Brien, C.A., Parathyroid hormone controls receptor activator of NF-kappaB ligand gene expression via a distant transcriptional enhancer (2006) Mol Cell Biol, 26, pp. 6453-6468; 
Gori, F., Hofbauer, L.C., Dunstan, C.R., Spelsberg, T.C., Khosla, S., Riggs, B.L., The expression of osteoprotegerin and RANK ligand and the support of osteoclast formation by stromal-osteoblast lineage cells is developmentally regulated (2000) Endocrinology, 141, pp. 4768-4776; 
Herzog, H., Neuropeptide Y and energy homeostasis: Insights from Y receptor knockout models (2003) Eur J Pharmacol, 480 (1-3), pp. 21-29; 
Hill, E.L., Turner, R., Elde, R., Effects of neonatal sympathectomy and capsaicin treatment on bone remodeling in rats (1991) Neuroscience, 44 (3), pp. 747-755; 
Holliday, N.D., Michel, M.C., Cox, H.M., NPY receptor subtypes and their signal transduction (2004) Neuropeptide Y and related peptides, Handbook of experimental pharmacology, 162, pp. 45-74. , Michel MC, editor, Berlin: Springer-Verlag, Heidelberg, pp; 
Hosaka, H., Nagata, A., Yoshida, T., Shibata, T., Nagao, T., Tanaka, T., Saito, Y., Tatsuno, I., Pancreatic polypeptide is secreted from and controls differentiation through its specific receptors in osteoblastic MC3T3-E1 cells (2008) Peptides, 29 (8), pp. 1390-1395; 
Imai, S., Matsusue, Y., Neuronal regulation of bone metabolism and anabolism: Calcitonin gene-related peptide-, substance P-, and tyrosine hydroxylase-containing nerves and the bone (2002) Microsc Res Tech, 58, pp. 61-69; 
Kondo, H., Guo, J., Bringhurst, F.R., Cyclic adenosine monophosphate/protein kinase A mediates parathyroid hormone/parathyroid hormone-related protein receptor regulation of osteoclastogenesis and expression of RANKL and osteoprotegerin mRNAs by marrow stromal cells (2002) J Bone Miner Res, 17, pp. 1667-1679; 
Kuo, L.E., Kitlinska, J.B., Tilan, J.U., Li, L., Baker, S.B., Johnson, M.D., Lee, E.W., Zukowska, Z., Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome (2007) Nat Med, 13, pp. 803-811; 
Lee, S.K., Lornezo, J.A., Regulation of receptor activator of nuclear factor-kappaB ligand and osteoprotegerin mRNA expression by parathyroid hormone is predominantly mediated by the protein kinase a pathway in murine bone marrow cultures (2002) Bone, 31, pp. 252-259; 
Lerner, U.H., Lundberg, P., Kinins, neuro-osteogenic factors (2002) Principles of bone biology, pp. 773-799. , Bilezikian JP, Raisz LG, Rodan GA, editors, 2nd edition. San Diego: Academic Press. pp; 
Li, J., Ahmad, T., Spetea, M., Ahmed, M., Kreicbergs, A., Bone reinnervation after fracture: A study in the rat (2001) J Bone Miner Res, 16, pp. 1505-1510; 
Lundberg, P., Lerner, U.H., Expression and regulatory role of receptors for vasoactive intestinal peptide in bone cells (2002) Microsc Res Tech, 58, pp. 98-103; 
Lundberg, P., Bostr{\"o}m, I., Mukohyama, H., Bjurholm, A., Smans, K., Lerner, U.H., Neuro-hormonal control of bone metabolism: Vasoactive intestinal peptide stimulates alkaline phosphatase activity and mRNA expression in mouse calvarial osteoblasts as well as calcium accumulation mineralized bone nodules (1999) Regul Pept, 85, pp. 47-58; 
Lundberg, P., Allison, S.J., Lee, N.J., Baldock, P.A., Brouard, N., Rost, S., Enriquez, R.F., Herzog, H., Greater bone formation of Y2 knockout mice is associated with increased osteoprogenitor numbers and altered Y1 receptor expression (2007) J Biol Chem, 282, pp. 19082-19091; 
Michel, M.C., Beck-Sickinger, A., Cox, H., Doods, H.N., Herzog, H., Larhammar, D., Quirion, R., Westfall, T., XVI. International Union of Pharmacology recommendations for the nomenclature of neuropeptide Y, peptide YY, and pancreatic polypeptide receptors (1998) Pharmacol Rev, 50, pp. 143-150; 
Movafagh, S., Hobson, J.P., Spiegel, S., Kleinman, H.K., Zukowska, Z., Neuropeptide Y induces migration, proliferation, and tube formation of endothelial cells bimodallyvia Y1, Y2, and Y5 receptors (2006) FASEB J, 20, pp. 1327-1337; 
Sainsbury, A., Baldock, P.A., Schwarzer, C., Ueno, N., Enriquez, R.F., Couzens, M., Inui, A., Gardiner, E.M., Synergistic effects of Y2 and Y4 receptors on adiposity and bone mass revealed in double knockout mice (2003) Mol Cell Biol, 23, pp. 5225-5233; 
Serre, C.M., Farlay, D., Delmas, P.D., Chenu, C., Evidence for a dense and intimate innervation of the bone tissue, including glutamate-containing fibers (1999) Bone, 25, pp. 623-629; 
Simonet, W.S., Lacey, D.L., Dunstan, C.R., Kelley, M., Chang, M.S., L{\"u}thy, R., Nguyen, H.Q., Boyle, W.J., Osteoprotegerin: A novel secreted protein involved in the regulation of bone density (1997) Cell, 89, pp. 309-319; 
Sudo, H., Kodama, H.A., Amagai, Y., Yamamoto, S., Kasai, S., In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria (1983) J Cell Biol, 96, pp. 191-198; 
Szczesny, G., Molecular aspects of bone healing, remodelling (2002) Pol J Pathol, 53, pp. 145-153; 
Tatemoto, K., Neuropeptide Y: Complete amino acid sequence of the brain peptide (1982) Proc Natl Acad Sci USA, 79, pp. 5485-5489; 
Thiriet, N., Deng, X., Solinas, M., Ladenheim, B., Curtis, W., Goldberg, S.R., Palmiter, R.D., Cadet, J.L., Neuropeptide Y protects against methamphetamine-induced neuronal apoptosis in the mouse striatum (2005) J Neurosci, 25, pp. 5273-5279; 
Wittmann, W., Loacker, S., Kapeller, I., Herzog, H., Schwarzer, C., Y1-receptors regulate the expression of Y2-receptors in distinct mouse forebrain areas (2005) Neuroscience, 136, pp. 241-250; 
Wortley, K.E., Garcia, K., Okamoto, H., Thabet, K., Anderson, K.D., Shen, V., Herman, J.P., Sleeman, M.W., Peptide YY regulates bone turnover in rodents (2007) Gastroenterology, 133, pp. 1534-1543
},
	month = {2009///},
	pages = {908 - 916},
	abstract = {Neuropeptide Y (NPY) has recently emerged as a potential regulator of bone homeostasis. However, the relevance of NPY{\textquoteright}s role in osteoblast activity and the biological functions involving NPY receptors in bone homeostasis remain to be clarified. Here we report that chronically elevated NPY levels leaded to a modulation of the level of Y2 receptor expression marked with a transient down and upregulation according to the stage of osteoblast differentiation. We also show that NPY is a negative regulator of Y1 receptor expression. The pharmalogical activation of Y2 receptor with its agonist resulted in similar effect. Functional analysis also revealed the osteogenic potential of NPY with osteoblast phenotype markers being significantly enhanced in osteoprogenitor cells stimulated by NPY, probably due to the down-regulation of Y1 receptor. In contrasts, these cells exhibit a reduction in calcium deposition in extracellular matrix most likely mediated via Y2 receptor signalling. Furthermore, we show that NPY modulates receptor activator of nuclear factor kB (NF-kB) (RANK) ligand and osteoprotegerin, two key factors regulating bone remodelling. Specifically, NPY inhibits the transcriptional activity of RANKL promoter in osteoprogenitor cells and enhances OPG expression in osteoblasts at early stages of differentiation. However, NPY effect on OPG seemed to be unrelated to Y2 receptor activation. Taken together the present data supported the contribution of NPY pathway in bone homeostasis via a direct action on osteoblasts cells. {\textcopyright} 2009 Wiley-Liss, Inc.},
	keywords = {alkaline phosphatase, animal cell, animal tissue, Animals, article, Bone, Bone Marrow Cells, bone remodeling, Calcium, cell activity, cell differentiation, cell function, Cell Line, cell stimulation, controlled study, down regulation, extracellular matrix, gene expression regulation, genetic transcription, Homeostasis, immunoglobulin enhancer binding protein, in vitro study, Mice, neuropeptide Y, neuropeptide Y1 receptor, neuropeptide Y2 receptor, nonhuman, NPY, nuclear factor Y, osteoblast, Osteoblasts, osteoclast differentiation factor, Osteoprotegerin, phenotype, priority journal, promoter region, protein expression, RANK Ligand, Receptors, Neuropeptide Y, Reverse Transcriptase Polymerase Chain Reaction, signal transduction, stem cell, Stromal Cells, upregulation, Y1 receptor, Y2 receptor},
	isbn = {07302312 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67749108153\&partnerID=40\&md5=3fac97e90b88592291766e499000ae54},
	author = {Teixeira, L. and Sousa, D.M. and Nunes, A.F. and Sousa, M.M. and Herzog, H. and Lamghari, M.}
}
@article {2309,
	title = {Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {89},
	year = {2009},
	note = {Cited By (since 1996): 11Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32011PubMed ID: 18442116Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, I. C.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Run do Campo Alegre, 823, 4150-180 Porto, Portugal; email: icastro@ineb.up.ptChemicals/CAS: fibrinogen, 9001-32-5; glutaraldehyde, 111-30-8, 37245-61-7; human serum albumin, 9048-49-1; Carbon, 7440-44-0; Ethylene Glycol, 107-21-1; Ligands; Serum AlbuminReferences: Nath, N., Hyun, J., Ma, H., Chilkoti, A., Surface engineering strategies for control of protein and cell interactions (2004) Surf Sci, 570, pp. 98-110;Mao, C., Qiu, Y.Z., Sang, H.B., Mei, H., Zhu, A.P., Shen, J., Lin, S.C., Various approaches to modify biomaterial surfaces for improving hemocompatibility (2004) Adv Colloid Interface Sci, 110, pp. 5-17; 
Gorbet, M.B., Sefton, M.V., Biomaterial-associated thrombosis: Roles of coagulation factors, complement, platelets and leukocytes (2004) Biomaterials, 25, pp. 5681-5703; 
Kottkemarchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., Effect of albumin coating on the in vitro blood compatibility of dacron arterial prostheses (1989) Biomaterials, 10, pp. 147-155; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., Alkyl substituted polymers with enhanced albumin affinity (1981) Trans Am Soc Artif Intern Organs, 27, pp. 499-503; 
Eberhart, R.C., Munro, M.S., Williams, G.B., Kulkarni, P.V., Shannon, W.A., Brink, B.E., Fry, W.J., Albumin adsorption and retention on C-18-alkyl-derivatized polyurethane vascular grafts (1987) Artif Organs, 11, pp. 375-382; 
Frautschi, J.R., Munro, M.S., Lloyd, D.R., Eberhart, R.C., Alkyl derivatized cellulose-acetate membranes with enhanced albumin affinity (1983) Trans Am Soc Artif Intern Organs, 29, pp. 242-244; 
Pitt WG, Cooper SL. Albumin adsorption on alkyl chain derivatized polyurethanes. I. The effect of C-18 alkylation. T Biomed Mater Res 1988;22:359-382Johnston, E., Ratner, B.D., Protein Adsorption: Friend or Foe? (1998) Immobilized biomolecules in analysis: A practical approach, pp. 79-94. , Cass T, Ligler FS, editors, Oxford: Oxford University Press;; 
Ji, J., Feng, L.X., Barbosa, M.A., Stearyl poly(ethylene oxide) grafted surfaces for preferential adsorption of albumin (2001) Biomaterials, 22, pp. 3015-3023; 
Martins, M.C.L., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on cibacron blue F3G-A immobilized onto oligo (ethylene glycol)-terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14, pp. 945-954; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) J Am Chem Soc, 120, pp. 3464-3473; 
Palegrosdemange, C., Simon, E.S., Prime, K.L., Whitesides, G.M., Formation of self-assembled monolayers by chemisorption of derivatives of oligo(ethylene glycol) of structure Hs(Ch2)11 (Och2ch2)meta-Oh on gold (1991) J Am Chem Soc, 113, pp. 12-20; 
Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-ter- minated self-assembled monolayers (2005) Biomaterials, 26, pp. 3891-3899; 
Martins MCL, Ratner BD, Barbosa MA. Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolavers. I Biomed Mater Res 2003;67A: 158-171Owens, D.K., Wendt, R.C., Estimation of surface free energy of polymers (1969) J Appl Polym Sci, 13, pp. 1741-1747; 
Sousa, S.R., Moradas Ferreira, P., Saramago, B., Viseu Melo, L., Barbosa, M.A., Human serum albumin adsorption on TiO 2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Sastry, M., A note on the use of ellipsometry for studying the kinetics of formation of self-assembled monolayers (2000) Bull Mater Sci, 23, pp. 159-163; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ralner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Frank, R.D., Dresbach, H., Thelen, H., Sieberth, H.G., Glutardialde- hyde induced fluorescence technique (GIFT): A new method for the imaging of platelet adhesion on biomaterials (2000) J Biomed Mater Res, 52, pp. 374-381; 
Herrwerth, S., Eck, W., Reinhardt, S., Grunze, M., Factors that determine the protein resistance of oligoether self-assembled monolayers-internal hydrophilicity, terminal hydrophilicity, and lateral packing density (2003) J Am Chem Soc, 125, pp. 9359-9366; 
Harder, P., Grunze, M., Dahint, R., Whitesides, G.M., Laibinis, P.E., Molecular conformation in oligo(ethylene glycol)-terminated self-assembled monolayers on gold and silver surfaces deter-mines their ability to resist protein adsorption (1998) J Phys Chem B, 102, pp. 426-436; 
Schwendel, D., Dahint, R., Herrwerth, S., Schloerholz, M., Eck, W., Grunze, M., Temperature dependence of the protein resistance of poly- and oligo(ethylene glycol)-terminated alkanethiolate monolayers (2001) Langmuir, 17, pp. 5717-5720; 
Zhu, B., Eurell, T., Gunawan, R., Leckband, D., Chain-length dependence of the protein and cell resistance of oligo(ethylene glycol)-terminated self-assembled monolayers on gold (2001) J Biomed Mater Res, 56, pp. 406-416; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (1999) J Biomed Mater Res, 49, pp. 435-447; 
Sperling, C., Schweiss, R.B., Streller, U., Werner, C., In vitro hemocompatibility of self-assembled monolayers displaying various functional groups (2005) Biomaterials, 26, pp. 6547-6557; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethio- lates on gold (2004) Biomaterials, 25, pp. 2557-2563
},
	month = {2009///},
	pages = {642 - 653},
	abstract = {This study focuses on the selective binding of albumin to a nanostructured surfaces to inhibit other blood proteins from adsorbing thereby reducing platelet adhesion and activation. Tetra (ethylene-glycol)-terminated self- assembled monolayers (EG4 SAMs) with different percentages of CI 8 ligands on the surface were characterized by contact angle measurements, X-ray photoelectron microscopy, infrared reflection-absorption spectroscopy, and ellipsometry. A specific surface (2.5\% C18 SAM) was found to be selective for human serum albumin (HSA) in the presence of both albumin and fibrinogen (HFG). The importance of this concentration of C18 ligands was stressed in reversibility studies since that surface exchanged almost all the preadsorbed HSA by HSA in solution, but not by HFG. The effect of protein adsorption in the subsequent adhesion and activation of platelets was studied by pre-immersing the surfaces in albumin and plasma before contact with platelets. Scanning electron microscopy and glutaraldehyde induced fluorescence technique images showed that as surfaces got more hydrophobic due to the immobilization of C18 ligands, the number of adherent platelets increased and their morphology changed from round to fully spread. Pre-immersion in HSA led to an 80{\textdegree}/o decrease in platelet adhesion and reduction of activation. Pre-immersion in 1\% plasma was only relevant in 2.5\% CI 8 SAMs since this was the only surface that demonstrated less adhesion of platelets comparing with buffer pre-immersion. However, they still adsorb more platelets then when HSA was preadsorbed. This was confirmed in competition studies between HSA and plasma that suggested that other plasma proteins were also adsorbing to this surface. {\textcopyright} 2008 Wiley Periodicals, Inc.},
	keywords = {Absorption spectroscopy, Acoustic microscopes, Adhesion, Adsorption, Albumin, Aldehydes, Angle measurement, article, Biological materials, Biomaterials, Blood Platelets, Blood protein, Blood substitutes, Carbon, Cell Shape, chemical composition, Concentration of, Contact angle, controlled study, Ethylene, Ethylene Glycol, ethylene glycol derivative, fibrinogen, Fluorescence technique, glutaraldehyde, gold nanoparticle, human, human serum albumin, Human serum albumins, Humans, hydrophobicity, Infrared reflection absorption spectroscopy, infrared spectroscopy, ligand, Ligands, Nanostructured surface, normal human, Oligo(ethylene glycol), Photoelectron spectroscopy, plasma, Plasma diagnostics, Plasma protein, Plasmas, Platelet adhesion, Platelet Adhesiveness, Platelets, Protein adsorption, protein binding, Proteins, Sams, Scanning electron microscopy, Selective binding, Selective protein adsorption, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, Serum Albumin, Specific surface, Spectrophotometry, Infrared, Surface Properties, surface property, thrombocyte activation, thrombocyte adhesion, thrombocyte shape, X ray photoelectron spectroscopy, X-ray photoelectron microscopy},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-66249128976\&partnerID=40\&md5=0be21ad44fe5cf556598975ea1655bed},
	author = {Gon{\c c}alves, I.C. and Martins, M.C.L. and Barbosa, M.A. and Naeemi, E. and Ratner, B.D.}
}
@article {2339,
	title = {Linear and nonlinear heart-rate analysis in a rat model of acute anoxia},
	journal = {Physiological MeasurementPhysiol. Meas.},
	volume = {29},
	year = {2008},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: PMEAEdoi: 10.1088/0967-3334/29/9/010PubMed ID: 18784391Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, H.; Departamento de Matem{\'a}tica Aplicada, Faculdade de Ci{\^e}ncias, Universidade Do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal; email: hernani.goncalves@fc.up.ptReferences: American College of Obstetricians and Gynecologists. ACOG Practice Bulletin. Clinical Management Guidelines for Obstetrician-Gynecologists, Number 70, December 2005 (2005) Obstet. Gynecol., 106, pp. 1453-1460;Ayres-De-Campos, D., Bernardes, J., Garrido, A., Marques-De-S{\'a}, J., Pereira-Leite, L., SisPorto 2.0: A program for automated analysis of cardiotocograms (2000) J. Mat. Fet. Med., 9 (5), pp. 311-318; 
Ayres-De-Campos, D., Santos, C., Bernardes, J., Prediction of neonatal state by computer analysis of fetal heart rate tracings: The antepartum arm of the SisPorto{\textregistered} validation study (2005) Eur. J. Obstet. Gynecol. Reprod. Biol., 118 (1), pp. 52-60; 
Beckers, F., Verheyden, B., Aubert, A.E., Aging and nonlinear heart rate control in a healthy population (2006) Am. J. Physiol. Heart Circ. Physiol., 290 (6), pp. 2560-H2570; 
Beckers, F., Verheyden, B., Ramaekers, D., Swinghedauw, B., Aubert, A.E., Effects of autonomic blockade on non-linear cardiovascular variability indices in rats (2006) Clin. Exp. Pharmacol. Physiol., 33 (5-6), pp. 431-439; 
Bernardes, J., Ayres-De-Campos, D., Costa-Pereira, A., Pereira-Leite, L., Garrido, A., Objective computerized fetal heart rate analysis (1998) Int. J. Gynecol. Obstet., 62 (2), pp. 141-147; 
Bernardes, J., Moura, C., Marques-De-S{\'a}, J.P., Pereira-Leite, L., The Porto system for automated cardiotocographic signal analysis (1991) J. Perinat. Med., 19, pp. 61-65; 
Cerutti, C., Gustin, M.P., Paultre, C.Z., Lo, M., Julien, C., Vincent, M., Sassard, J., Autonomic nervous-system and cardiovascular variability in rats-a spectral-analysis approach (1991) Am. J. Physiol., 261, pp. 1292-H1299; 
Chaffin, D.G., Barnard, J.M., Phernetton, T., Reed, K.L., Decreased approximate entropy of heart rate variability in the hypoxic ovine fetus (1998) J. Matern. Fetal Invest., 8, pp. 23-26; 
Dudewicz, E., Mishra, S., (1988) Modern Mathematical Statistics; 
Felgueiras, C., Marques-De-S{\'a}, J.P., Bernardes, J., Gama, S., Fractal characterisation of fetal heart rate sequences (1998) Biomedical Eng., 36, pp. 197-201; 
Gon{\c c}alves, H., Bernardes, J., Rocha, A.P., Ayres-De-Campos, D., Linear and nonlinear analysis of heart rate patterns associated with fetal behavioral states in the antepartum period (2007) Early Human Develop., 83 (9), pp. 585-591; 
Gon{\c c}alves, H., Rocha, A.P., Ayres-De-Campos, D., Bernardes, J., Internal versus external intrapartum foetal heart rate monitoring: The effect on linear and nonlinear parameters (2006) Physiol. Meas., 27 (3), pp. 307-319; 
Gon{\c c}alves, H., Rocha, A.P., Ayres-De-Campos, D., Bernardes, J., Linear and nonlinear fetal heart rate analysis of normal and acidemic fetuses in the minutes preceding delivery (2006) Med. Biol. Eng. Comput., 44 (10), pp. 847-855; 
Hayes, M., (1996) Statistical Digital Signal Processing and Modelling; 
Henriques-Coelho, T., Correia-Pinto, J., Roncon-Albuquerque, R., Baptista, M.J., Lourenco, A.P., Oliveira, S.M., Brandao-Nogueira, A., Leite-Moreira, A.F., Endogenous production of ghrelin and beneficial effects of its exogenous administration in monocrotaline-induced pulmonary hypertension (2004) Am. J. Physiol. Heart Circ. Physiol., 287 (6), pp. 2885-H2890; 
Kuwahara, M., Yayou, K., Ishii, K., Hashimoto, S., Tsubone, H., Sugano, S., Power spectral analysis of heart rate variability as a new method for assessing autonomic activity in the rat (1994) J. Electrocardiology, 27 (4), pp. 333-337; 
Li, X., Tang, D., Zhou, S., Zhou, G., Wang, C., Zhuang, Y., Wu, G., Shen, L., Redistribution of power spectrum of heart rate variability during acute umbilical artery embolism and hypoxemia in late-gestation fetal sheep (2004) Eur. J. Obstet. Gynecol. Reprod. Biol., 114 (2), pp. 137-143; 
Louren{\c c}o, A.P., Roncon-Albuquerque, R., Bras-Silva, C., Faria, B., Wieland, J., Henriques-Coelho, T., Correia-Pinto, J., Leite-Moreira, A.F., Myocardial dysfunction and neurohumoral activation without remodeling in left ventricle of monocrotaline-induced pulmonary hypertensive rats (2006) Am. J. Physiol. Heart Circ. Physiol., 291 (4), pp. 1587-H1594; 
Magenes, G., Signorini, M.G., Arduini, D., Classification of cardiotocographic records by neural networks (2000) IJCNN 2000: Proc. IEEE-INNS-ENNS Int. Joint Conf. on Neural Network, 3, pp. 637-641; 
Martinez, W.L., Martinez, A.R., (2002) Computational Statistics Handbook with MATLAB; 
Moraru, L., Cimponeriu, L., Tong, S., Thakor, N., Bezerianos, A., Characterization of heart rate variability changes following asphyxia in rats (2004) Methods Inf. Med., 43 (1), pp. 118-121; 
Moraru, L., Tong, S., Malhotra, A., Geocadin, R., Thakor, N., Bezerianos, A., Investigation of the effects of ischemic preconditioning on the HRV response to transient global ischemia using linear and nonlinear methods (2005) Med. Eng. Phys., 27 (6), pp. 465-473; 
Murasato, Y., Hirakawa, H., Harada, Y., Nakamura, T., Hayashida, Y., Effects of systemic hypoxia on R-r interval and blood pressure variabilities in conscious rats (1998) Am. J. Physiol., 275, pp. 797-H804; 
Niklasson, U., Wiklund, U., Bjerle, P., Olofsson, B.O., Heart-rate variation-what are we measuring? (1993) Clin. Physiol., 13 (1), pp. 71-79; 
Pincus, S., Aproximate entropy (ApEn) as a complexity measure (1995) Chaos, 5 (1), pp. 110-117; 
Pincus, S.M., Gevers, E.F., Robinson, I.C.A.F., Vandenberg, G., Roelfsema, F., Hartman, M.L., Veldhuis, J.D., Females secrete growth hormone with more process irregularity than males in both humans and rats (1996) Am. J. Physiol., 270, pp. 107-E115; 
Pincus, S., Viscarello, R., Approximate Entropy: A regularity measure for fetal heart rate analysis (1992) Obstet. Gynecol., 79, pp. 249-255; 
Richman, J.S., Moorman, J.R., Physiological time-series analysis using approximate entropy and sample entropy (2000) Am. J. Physiol. Heart Circ. Physiol., 278, pp. 2039-H2049; 
Signorini, M.G., Magenes, G., Cerrutti, S., Arduini, D., Linear and nonlinear parameters for the analysis of fetal heart rate signal from cardiotacographic recordings (2003) IEEE Trans. Biomed. Eng., 50 (3), pp. 365-373; 
Siira, S.M., Ojala, T.H., Vahlberg, T.J., Jalonen, J.O., Valimaki, I.A., Rosen, K.G., Ekholm, E.M., Marked fetal acidosis and specific changes in power spectrum analysis of fetal heart rate variability recorded during the last hour of labour (2005) BJOG, 112 (4), pp. 418-423; 
Task force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology, Heart rate variability-standards of measurement, physiological interpretation and clinical use (1996) Circulation, 93, pp. 1043-1065; 
Vieira, P., Marques-De-S{\'a}, J.P., Bernardes, J., Fractal analysis of foetal heart rate (2004) Proc. Biosignal 2004, pp. 53-55
},
	month = {2008///},
	pages = {1133 - 1143},
	abstract = {The objective of this study was the assessment of heart-rate (HR) dynamics with linear and nonlinear methods during episodes of mechanical ventilation and acute anoxia in rats. Namely, to assess whether linear and nonlinear HR analysis was able to discriminate acute anoxia from baseline in rats and if this was consistent with human foetal and adult studies. Five HR segments of 1 min duration, during baseline recording, mechanical ventilation and first, second and third minutes of induced acute anoxia, were analysed in ten adult Wistar rats. Linear time and frequency domain and nonlinear methods were used, namely mean HR (mHR), long-term irregularity (LTI), interval index (II), low frequency (LF) and high frequency (HF), approximate entropy (ApEn) and sample entropy (SampEn). New parameters for the entropy indices are proposed for the analysis of rats{\textquoteright} HR. Bootstrap percentile confidence intervals and nonparametric statistical tests were used in the evaluation of the differences between segments. During mechanical ventilation a clear spectral band was detectable at the ventilation rate, but mHR, II and the {\textquoteright}new{\textquoteright} entropy indices were the only significantly changed indices. In the transition from baseline-mechanical- ventilation to mechanical-ventilation-induced anoxia, a statistically significant decrease of mHR, II and entropy indices was observed, clearly discriminating these two instances, whereas most linear indices increased. With continued anoxia, most linear indices decreased significantly, whereas entropy remained stably low. These results are consistent with other foetal human and non-human studies and evidence that the rat model may be used for further research on linear and nonlinear analysis of heart-rate dynamics. The transition from baseline to acute anoxia was encompassed by signs of increased activation of the autonomic nervous system sympathetic branch, and decreased or blunted activity of the HR complexity regulatory centres. {\textcopyright} 2008 Institute of Physics and Engineering in Medicine.},
	keywords = {acute disease, animal experiment, animal model, Animals, Anoxia, article, artificial ventilation, bootstrapping, controlled study, entropy, Female, Fetal monitoring, frequency analysis, Heart rate, Heart rate variability, Humans, Linear analysis, linear system, lung ventilation, Models, Cardiovascular, nonhuman, nonlinear system, priority journal, rat, Rats, Rats, Wistar, Rattus, Rattus norvegicus, Respiration, Artificial, Statistical analysis, Variability, Wistar rat, Wistar rats},
	isbn = {09673334 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-51849120545\&partnerID=40\&md5=56be327bb0d959060a2f4a0f080ae264},
	author = {Gon{\c c}alves, H. and Henriques-Coelho, T. and Bernardes, J. and Rocha, A.P. and Nogueira, A. and Leite-Moreira, A.}
}
@article {2351,
	title = {Surface characterization and cell response of a PLA/CaP glass biodegradable composite material},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {85},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31546PubMed ID: 17729262Language of Original Document: EnglishCorrespondence Address: Ginebra, M. P.; Department of Materials Science, Institute of Bioengineering of Catalonia (IBEC), Technical University of Catalonia, Avda Diagonal 647, 08028-Barcelona, Spain; email: maria.pau.ginebra@upc.eduChemicals/CAS: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, 298-93-1; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; osteocalcin, 136461-80-8; polylactic acid, 26100-51-6; Alkaline Phosphatase, EC 3.1.3.1; Bone Substitutes; calcium phosphate, 10103-46-5; Calcium Phosphates; Lactic Acid, 50-21-5; Osteocalcin, 104982-03-8; poly(lactic acid), 26100-51-6; PolymersReferences: Barrows, T., Degradable implants materials: A review of synthetic absorbable polymers and their applications (1986) Clin Mater, 1, pp. 233-267;Ciccone, W., Motz, C., Bentley, C., Tasto, J., Bioabsorbable implants in orthopaedics: New developments and clinical applications (2001) J Am Acad Orthop Surg, 9, pp. 280-288; 
Dunn, R.L., Clinical applications and update on the poly (alpha-hydroxy acids) (1995) Biomedical Applications of Synthetic Biodegradable Polymers, pp. 17-31. , Hollinger JO, editor, Boca Raton: CRC Press;; 
Middleton, J.C., Tipton, A.J., Synthetic biodegradable polymers as orthopedic devices (2000) Biomaterials, 21, pp. 2335-2346; 
B{\"o}stman O Current concepts review. Absorbable implants for fracture fixation. J Bone Joint Surgery 1991;73A:148-153Ashammakhi, N., Suuronen, R., Tiainen, J., Tormala, P., Waris, T., Spotlight on naturally absorbable osteofixation devices (2003) J Craniofac Surg, 14, pp. 247-259; 
Hou, Q.P., Grijpma, D.W., Feijen, J., Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique (2003) Biomaterials, 24, pp. 1937-1947; 
Kellomaki, M., Niiranen, H., Puumanen, K., Ashammakhi, N., Waris, T., Tormala, P., Bioabsorbable scaffolds for guided bone regeneration and generation (2000) Biomaterials, 21, pp. 2495-2505; 
Mooney, D.J., Park, S., Kaufmann, P.M., Sano, K., Mcnamara, K., Vacanti, J.P., Langer, R., Biodegradable sponges for hepatocyte transplantation (1995) J Biomed Mater Res, 29, pp. 959-965; 
Ragel, C.V., Vallet-Regi, M., In vitro bioactivity and gentamicin release from glass-polymer-antibiotic composites (2000) J Biomed Mater Res, 51, pp. 424-429; 
Kohn, J., Langer, R., Bioabsorbable and bioerodible materials (1996) Biomaterials Science, pp. 64-72. , Ratner BD, Hoffman A, Schoen F, Lemons J, editors, New York: Academic Press;; 
Navarro, M., Ginebra, M.P., Clement, J., Martinez, S., Avila, G., Planell, J.A., Physicochemical degradation of titania-stabilized soluble phosphate glasses for medical applications (2003) J Am Ceram Soc, 86, pp. 1345-1352; 
Navarro, M., Ginebra, M.P., Planell, J.A., Barrias, C.C., Barbosa, M., In vitro degradation behavior of a novel bioabsorbable composite material based on PLA and a soluble CaP glass (2005) Acta Biomaterialia, 1, pp. 411-419; 
Stephansson, S.N., Byers, B.A., Garcia, A.J., Enhanced expression of the osteoblastic phenotype on substrates that modulate fibronectin conformation and integrin receptor binding (2002) Biomaterials, 23, pp. 2527-2534; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res A, 66, pp. 247-259; 
Beumer, G., van Blitterswijk, C., Bakker, D., Ponec, M., Cell-seeding and in vitro biocompatibility evaluation of polymeric matrices of PEO/PBT copolymers and PLLA (1993) Biomaterials, 14, pp. 598-604; 
Ishaug, S., Yaszemski, M., Bizios, R., Mikos, A., Osteoblast function on synthetic biodegradable polymers (1994) J Biomed Mater Res, 28, pp. 1445-1453; 
Navarro, M., Ginebra, M.P., Planell, J.A., Cellular response to calcium phosphate glasses with controlled solubility (2003) J Biomed Mater Res A, 67, pp. 1009-1015; 
Huffman, K.R., Casey, D.J., Effect of carboxyl end groups on hydrolysis of polyglycolic acid (1985) J Polym Sci A: Polym Chem, 23, pp. 1939-1954; 
Ishaug-Riley, S.L., Okun, L.E., Prado, G., Applegate, M.A., Ratcliffe, A., Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films (1999) Biomaterials, 20, pp. 2245-2256; 
Park, A., Griffith, L., In vitro cell response to differences in poly-1-lactide crystallinity (1996) Biomaterials, 31, pp. 117-130; 
Cai, K.Y., Yao, K.D., Lin, S.B., Yang, Z.M., Li, X.Q., Xie, H.Q., Poly (D, L-lactic acid) surfaces modified by silk fibroin: Effects on the culture of osteoblast in vitro (2002) Biomaterials, 23, pp. 1153-1160; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tension, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Vogler, E.A., Structure and reactivity of water at biomaterial surfaces (1998) Adv Colloid Interface Sci, 74, pp. 69-117; 
Vogler, E.A., Interfacial chemistry in biomaterials science (1993) Surfactant Science Series, pp. 183-250. , Berg JC, editor. Wettability, Marcel Dekker, New York; 
Charles-Harris, M., Navarro, M., Engel, E., Aparicio, C., Ginebra, M.P., Planell, J., A Surface characterisation of completely degradable composite scaffolds (2005) J Mater Svi: Mater Med, 16, pp. 1125-1130; 
Lampin, M., WarocquierClerout, R., Legris, C., Degrange, M., SigotLuizard, M.F., Correlation between substratum roughness and wettability, cell adhesion, and cell migration (1997) J Biomed Mater Res, 36, pp. 99-108; 
Khang, G., Jeon, J.H., Lee, L.W., Cho, S.C., Lee, H.B., Cell and platlet adhesion on plasma glow discharge-treated poly(lactide-co-glycolide) (1997) Biomed Mater Engl, 7, pp. 357-368; 
Webb, K., Hlady, V., Tresco, P.A., Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization (1998) J Biomed Mater Res, 41, pp. 422-430; 
Owen, T.A., Aronow, M., Shalhoub, V., Barone, L.M., Wilming, L., Tassinari, M.S., Progressive development of the rat osteoblast phenotype in vitro - Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular-matrix (1990) J Cell Physiol, 143, pp. 420-430; 
Lian, J.B., Stein, G.S., Concepts of osteoblast growth and differentiation - Basis for modulation of bone cell-development and tissue formation (1992) Crit Rev Oral Biol Med, 3, pp. 269-305; 
Quarles, L.D., Yohay, D.A., Lever, L.W., Caton, R., Wenstrup, R.J., Distinct proliferative and differentiated stages of murine Mc3T3-E1 cells in culture - An in vitro model of osteoblast development (1992) J Bone Miner Res, 7, pp. 683-692; 
Boyan, B.D., Dean, D.D., Lohmann, C.H., Cochran, D.L., Sylvia, V.L., Schwartz, Z., The titanium-bone cell interface in vitro: The role of the surface in promoting osteointegration (2001) Titanium in Medicine, pp. 562-585. , Brunette D, Tengvall P, Textor M, Thomsen P, editors, Berlin: Springer-Verlag;; 
Gronowicz, G., McCarthy, M.B., Ahmad, M., Direct integrin-mediated attachment of human osteoblasts to implants (1996) J Bone Miner Res, 11, pp. S323; 
Boyan, B.D., Hummert, T.W., Kieswetter, K., Schraub, D., Dean, D.D., Schwartz, Z., Effect of titanium surface characteristics on chondrocytes and osteoblasts in vitro (1995) Scan Electron Microsc, 5, pp. 323-335; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Effect of titanium surface-roughness on proliferation. Differentiation, and protein-synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401
},
	month = {2008///},
	pages = {477 - 486},
	abstract = {Bioabsorbable materials are of great interest for bone regeneration applications, since they are able to degrade gradually as new tissue is formed. In this work, a fully biodegradable composite material containing polylactic acid (PLA) and calcium phosphate (CaP) soluble glass particles has been characterized in terms of surface properties and cell response. Cell cultures were performed in direct contact with the materials and also with their extracts, and were evaluated using the MTT assay, alkaline phosphatase activity, and osteocalcin measurements. The CaP glass and PLA were used as reference materials. No significant differences were observed in cell proliferation with the extracts containing the degradation by-products of the three materials studied. A relation between the materials wettability and the material-cell interactions at the initial stages of contact was observed. The most hydrophilic material (CaP glass) presented the highest cell adhesion values as well as an earlier differentiation, followed by the PLA/glass material. The incorporation of glass particles into the PLA matrix increased surface roughness. SEM images showed that the heterogeneity of the composite material induced morphological changes in the cells cytoskeleton. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, Absorbable Implants, alkaline phosphatase, article, Biodegradable composite materials, biodegradable implant, Biodegradation, Bone, Bone Regeneration, Bone Substitutes, calcium phosphate, Calcium phosphates, cell adhesion, Cell culture, cell function, cell interaction, Cell Line, cell proliferation, Cell response, Composite materials, cytoskeleton, glass, human, human cell, Humans, hydrophilicity, hydrophobicity, In vitro test, Lactic Acid, materials testing, Osteoblasts, osteocalcin, polylactic acid, Polymers, scanning electron microscope, Surface analysis, Surface Properties, wettability},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-42149187233\&partnerID=40\&md5=8942b2e00b94ff0f723261e1893d04b5},
	author = {Navarro, M. and Engel, E. and Planell, J.A. and Amaral, I. and Barbosa, M. and Ginebra, M.P.}
}
@article {2450,
	title = {In vitro degradation behavior of a novel bioresorbable composite material based on PLA and a soluble CaP glass},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {1},
	year = {2005},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2005.03.004PubMed ID: 16701822Language of Original Document: EnglishCorrespondence Address: Ginebra, M.P.; Reference Centre for Bioengineering of Catalonia (CREBEC), Department of Materials Science, Universitat Polit{\`e}cnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, Spain; email: maria.pau.ginebra@upc.esChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; polylactic acid, 26100-51-6; Bone Substitutes; calcium phosphate, 10103-46-5; Calcium Phosphates; Lactic Acid, 50-21-5; poly(lactic acid), 26100-51-6; PolymersManufacturers: Purac, NetherlandsReferences: Rokkanen, P., Bioabsorbable fixation in orthopaedic surgery and traumatology (2000) Biomaterials, 21, pp. 2607-2613;Middleton, J.C., Tipton, A.J., Synthetic biodegradable polymers as orthopedic devices (2000) Biomaterials, 21 (23), pp. 2335-2346; 
Adriano, K.P., Daniels, A.U., Smutz, W.P., Wyatt, R.W.B., Heller, J., Preliminary biocompatibility screening of several biodegradable phosphate fiber reinforced polymers (1993) J Appl Biomater, 4, pp. 1-12; 
Nazhat, S.N., Kellomaki, M., Tormala, P., Tanner, K.E., Bonfield, W., Dynamic mechanical characterization of biodegradable composites of hydroxyapatite and polylactides (2001) J Biomed Mater Res, 58 (4), pp. 335-343; 
Dupraz, A.M.P., de Wijn, J.R., v.d. Meer, S.A.T., de Groot, K., Characterization of silane-treated hydroxyapatite powders for use as filler in biodegradable composites (1996) J Biomed Mater Res, 30, pp. 231-238; 
Kasuga, T., Maeda, H., Kato, K., Nogami, M., Hata, K.-I., Ueda, M., Preparation of poly (lactic acid) composites containing calcium carbonate (vaterite) (2003) Biomaterials, 24, pp. 3247-3253; 
Ylinen, P., Suuronen, R., Taurio, R., Tormala, P., Rokkanen, P., Use of hydroxylapatite/polymer-composite in facial bone augmentation. An experimental study (2002) Int J Oral Maxillofac Surg, 31 (4), pp. 405-409; 
Lu, H.H., El-Amin, S., Scott, K., Laurencin, C., Three-dimensional, bioactive, biodegradable, polymer-bioactive glass composite scaffolds with improved mechanical properties support collagen synthesis and mineralization of human osteoblast-like cells in vitro (2003) J Biomed Mater Res, 64 A, pp. 465-474; 
Navarro, M., Ginebra, M.P., Planell, J.A., Zeppetelli, S., Ambrosio, L., Development and cell response of a new biodegradable composite scaffold for guided bone regeneration (2004) J Mater Sci-Mater Med, 15 (4), pp. 419-422; 
Klein, C.P.A.T., Driessen, A.A., Degroot, K., Vandenhooff, A., Biodegradation behavior of various calcium-phosphate materials in bone tissue (1983) J Biomed Mater Res, 17 (5), pp. 769-784; 
Denissen, H.W., Degroot, K., Makkes, P.C., Vandenhooff, A., Klopper, P.J., Tissue-response to dense apatite implants in rats (1980) J Biomed Mater Res, 14 (6), pp. 713-721; 
Navarro, M., Ginebra, M.P., Clement, J., Martinez, S., Avila, G., Planell, J.A., Physicochemical degradation of titania-stabilized soluble phosphate glasses for medical applications (2003) J Am Ceram Soc, 86 (8), pp. 1345-1352; 
Navarro, M., Ginebra, M.P., Planell, J.A., Cellular response to calcium phosphate glasses with controlled solubility (2003) J Biomed Mater Res Part A, 67 A (3), pp. 1009-1015; 
Li, S.M., Hydrolytic degradation characteristics of aliphatic polyesters derived from lactic and glycolic acids (1999) J Biomed Mater Res, 48 (3), pp. 342-353; 
Burg, K.J.L., Shalaby, S.W., Physicochemical changes in degrading polylactide films (1997) J Biomat Sci Polym, 9 (1), pp. 15-29; 
Li, S.M., Vert, M., Hydrolytic degradation of coral/poly(DL-lactic acid) bioresorbable material (1996) J Biomat Sci-Polym, 7 (9), pp. 817-827; 
Agrawal, C.M., Athanasiou, K.A., Technique to control pH in vicinity of biodegrading PLA-PGA implants (1997) J Biomed Mater Res, 38 (2), pp. 105-114; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., Solutions able to reproduce invivo surface-structure changes in bioactive glass-ceramic A-W3 (1990) J Biomed Mater Res, 24 (6), pp. 721-734; 
Fischer, E.W., Sterzel, H.J., Wegner, G., Investigation of the structure of solution grown crystals of lactide copolymers by means of chemical reactions (1973) Kolloid-Zeitschrift and Z Polymere, 251, pp. 978-990; 
Driessens, F.C.M., Fernandez, E., Ginebra, M.P., Boltong, M.G., Planell, J.A., Calcium phosphates and ceramic bone cements vs. acrylic cements (1997) Anales Quimica, 93 (1), pp. S38-S43; 
Berry, E.E., Baddiel, C.B., Some assignments in infra-red spectrum of octacalcium phosphate (1967) Spectrochim Acta Part A - Mol Spectrosc A, 23 (6), p. 1781; 
Fowler, B.O., Moreno, E.C., Brown, W.E., Infra-red spectra of hydroxyapatite octacalcium phosphate and pyrolysed octacalcium phosphate (1966) Arch Oral Biol, 11 (5), p. 477; 
Elliot, J.C., General chemistry of the calcium orthophosphates. Structure and chemistry of the apatites and other calcium orthophosphates (1994), pp. 1-61. , Elsevier AmsterdamStutman, J.M., Termine, J.D., Posner, A.S., Vibrational spectra and structure of phosphate ion in some calcium phosphates (1965) Trans New York Acad Sci, 27 (6), p. 669; 
Cornilsen, B.C., Condrate, R.A., The vibrational spectra of β-Ca 2P 2 O 7 and γ-Ca 2P 2O 7 (1979) J Inorg Nucl Chem, 41, pp. 602-605; 
Cornilsen, B.C., Condrate, R.A., Vibrational spectra of α-alkaline earth pyrophosphates (1978) J Solid State Chem, 23, pp. 375-382; 
de F{\'a}tima Gimenez, I., Mazali, I.O., Alves, O.L., Application of Raman spectroscopy to the study of the phase composition of phosphate based glass-ceramics (2001) J Phys Chem Solids, 62, pp. 1251-1255; 
Kasuga, T., Abe, Y., Calcium phosphate invert glasses with soda and titania (1999) J Non-Cryst Solids, 243, pp. 70-74; 
Imai, Y., Fukuzawa, A., Watanabe, M., Effect of blending tricalcium phosphate on hydrolytic degradation of a block polyester containing poly(L-lactic acid) segment (1999) J Biomat Sci - Polym, 10 (7), pp. 773-786; 
Huffman, K.R., Casey, D.J., Effect of carboxyl end groups on hydrolysis of polyglycolic acid (1985) J Polym Sci Part A - Polym Chem, 23 (7), pp. 1939-1954; 
Termine, J.D., Eanes, E.D., Comparative chemistry of amorphous and apatitic calcium phosphate preparations (1972) Calcif Tissue Res, 10 (3), p. 171; 
Zahidi, E., Lebugle, A., Bonel, G., On a new class of biomaterials for osseous or dental prosthesis (1985) Bull Soc Chim France, (4), pp. 523-527; 
Eanes, E.D., Gillesse, I.H., Gosner, A.S., Intermediate states in precipitation of hydroxyapatite (1965) Nature, 208 (5008), p. 365; 
Tung, M.S., Brown, W.E., An intermediate state in hydrolysis of amorphous calcium-phosphate (1983) Calcif Tissue Int, 35 (6), pp. 783-790; 
Wuthier, R.E., Rice, G.S., Wallace, J.E.B., Weaver, R.L., Legeros, R.Z., Eanes, E.D., Invitro precipitation of calcium-phosphate under intracellular conditions - Formation of brushite from an amorphous precursor in the absence of Atp (1985) Calcif Tissue Int, 37 (4), pp. 401-410; 
Root, M.J., Inhibition of the amorphous calcium-phosphate phase-transformation reaction by polyphosphates and metal-ions (1990) Calcif Tissue Int, 47 (2), pp. 112-116; 
},
	month = {2005///},
	pages = {411 - 419},
	abstract = {Poly(α-hydroxy acids), and in particular polylactic acid (PLA), are nowadays amongst the most used bioabsorbable materials. However, this polymer may not meet some application requirements due to inadequate mechanical properties and or its degradation characteristics. A possible strategy to tackle this problem is the incorporation of an inorganic phase into the polymeric matrix. In this work a new fully biodegradable composite material made with PLA and calcium phosphate soluble glass particles has been developed. The behaviour of the PLA/ glass composite has been analysed during its degradation in simulated physiological conditions by means of weight loss, molecular weight and thermal properties analysis and electron microscopy observation. The results showed that the incorporation of phosphate glass particles into the polymer significantly accelerated the degradation of the PLA and induced the formation of calcium phosphate precipitates at the composite surface. {\textcopyright} 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Absorbable Implants, Absorption, article, biodegradability, Biodegradable composite, Biomimetic materials, Body fluids, Bone Regeneration, Bone Substitutes, calcium phosphate, Calcium phosphates, composite material, Degradation, electron microscopy, glass, In vitro degradation, in vitro study, Lactic Acid, materials testing, molecular weight, observation, Phosphate glass, polylactic acid, Polymers, precipitation, priority journal, reaction analysis, simulation, Solubility, Surface Properties, surface property, temperature, weight reduction},
	isbn = {17427061 (ISSN)},
	author = {Navarro, M. and Ginebra, M.P. and Planell, J.A. and Barrias, C.C. and Barbosa, M.A.}
}
@conference {2446,
	title = {Spectral methods in image segmentation: A combined approach},
	booktitle = {Lecture Notes in Computer ScienceLect. Notes Comput. Sci.},
	series = {Second Iberian Conference on Pattern Recognition and Image Analysis, IbPRIA 2005},
	volume = {3523},
	year = {2005},
	note = {Conference code: 65649Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Monteiro, F.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Campus de Santa Apol{\'o}nia, Apartado 134, 5301-857 Bragan{\c c}a, Portugal; email: monteiro@ipb.ptReferences: Fowlkes, C., Belongie, S., Chung, F., Malik, J., Spectral grouping using nystr{\"o}m method (2004) Trans. on Pattern Analysis and Machine Intelligence, 26 (2), pp. 214-225;Malik, J., Belongie, S., Leung, T., Shi, T., Contour and texture analysis for image segmentation (2001) International Journal of Computer Vision, 43 (1), pp. 7-27; 
Martin, D., Fowlkes, C., Tal, D., Malik, J., A database of human segmented natural images and its application to evaluating segmentation algorithms and measuring ecological statistics (2001) IEEE Int. Conf. on Computer Vision, 2, pp. 416-424; 
Meila, M., Shi, J., Learning segmentation by random walks (2000) Neural Information Processing Systems, pp. 873-879; 
Ng, A., Jordan, M., Weiss, Y., On spectral clustering: Analysis and an algorithm (2002) Advances in Neural Information Processing Systems, 14, pp. 849-856; 
Scott, D.S., Solving sparse symmetric generalized eigenvalue without factorization (1981) SIAM Journal of Numerical Analisys, 18, pp. 102-110. , http://www.netlib.org/laso/snlaso.f; 
Scott, G.L., Longuet-Higgins, H.C., Feature grouping by "relocalisation" of eigenvectors of the proximity matrix (1990) Proc. British Machine Vision Conf., pp. 103-108; 
Shi, J., Malik, J., Normalized cuts and image segmentation (2000) IEEE Trans. on Pattern Analysis and Machine Intelligence, 22 (8), pp. 888-905; 
Weiss, Y., Segmentation using eigenvectors: A unifying view (1999) International Conference on Computer Vision, pp. 975-982; 
Wertheimer, M., Laws of organization in perceptual forms (1938) A Sourcebook of Gestalt Psychology, pp. 71-88. , W. B. Ellis Editor; 
Wu, Z., Leahy, R., An optimal graph theoretic approach to data clustering: Theory and its application to image segmentation (1993) IEEE Trans. on Pattern Analysis and Machine Intelligence, 15, pp. 1101-1113; 
Yu, S., Shi, J., Multiclass spectral clustering (2003) International Conference on Computer Vision, pp. 313-319. , Nice, France
},
	month = {2005///},
	pages = {191 - 198},
	address = {Estoril},
	abstract = {Grouping and segmentation of images remains a challenging problem in computer vision. Recently, a number of authors have demonstrated a good performance on this task using spectral methods that are based on the eigensolution of a similarity matrix. In this paper, we implement a variation of the existing methods that combines aspects from several of the best-known eigenvector segmentation algorithms to produce a discrete optimal solution of the relaxed continuous eigensolution. {\textcopyright} Springer-Verlag Berlin Heidelberg 2005.},
	keywords = {Algorithms, Computer vision, Discrete optimal solution, Eigensolution, Eigenvalues and eigenfunctions, Image segmentation, Matrix algebra, Problem solving, Similarity matrix, Spectral methods},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-25144437374\&partnerID=40\&md5=5b6351af4ac58a16c41b5558a48ae9e2},
	author = {Monteiro, F.C. and Campilho, A.C.},
	editor = {Marques J.S. and Perez de la Blanca N. and Pina P.}
}
@article {2509,
	title = {Albumin adsorption on Cibacron Blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {14},
	year = {2003},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1026394431100Language of Original Document: EnglishCorrespondence Address: Martins, Ma.C.L.; INEB - Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: cibacron blue f3ga, 12236-82-7, 53127-08-5; ethylene glycol, 107-21-1; fibrinogen, 9001-32-5; gold, 7440-57-5; human serum albumin, 9048-49-1; iodine 125, 14158-31-7, 22822-81-7; macrogol, 25322-68-3; thiol derivative, 13940-21-1; water, 7732-18-5References: Ratner, B.D., (1996) J. Mol. Recognit., 9, p. 617;Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., (1998) Biomaterials, 10, p. 147; 
Dequeiroz, A.A.A., Barrak, E.R., Gil, H.A.C., Higa, O.Z., (1997) J. Biomater. Sci. Polym. Ed., 8, p. 667; 
Kamath, K.R., Park, K., (1994) J. Appl. Biomater., 5, p. 163; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., (1981) Trans. Am. Soc. Artif. Intern. Organs, 27, p. 499; 
Ji, J., Feng, L., Barbosa, M.A., (2001) Biomaterials, 22, p. 3015; 
Keogh, J.R., Eaton, J.W., (1994) J. Lab. Clin. Med., 121, p. 537; 
Wang, D., Chen, B., Ji, J., Feng, L., (2002) Bioconjugate Chem., 13, p. 792; 
Ji, J., Barbosa, M.A., Feng, L., Shen, J., (2002) J. Mat. Sci. Mat. Med., 13, p. 677; 
Mcfarland, C.D., Jenkins, M., Griesser, H.J., Chatelier, R.C., Steele, J.G., Underwood, P.A., (1998) J. Biomater. Sci. Polym. Ed., 9, p. 1207; 
Mcfarland, C.D., Filippis, C.D., Jenkins, M., Tunstell, A., Rhodes, N.P., Williams, D.F., Steele, J.G., (1998) J. Biomater. Sci. Polym. Ed., 9, p. 1277; 
Kopperschlager, G., Bohme, H.J., Hofmann, E., (1982) Adv. Biochem. Eng., 25, p. 101; 
Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannell, R., (1976) Biochem. J., 157, p. 301; 
Leatherbarrow, R.L., Dean, P.D.G., (1980) Biochem. J., 189, p. 27; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., (2003) J. Biomater. Sci. Polym. Ed., 14, p. 439; 
Dockal, M., Carter, D.C., Ruker, F., (1999) J. Biol. Chem., 274, p. 29303; 
Ostuni, E., Yan, L., Whitesides, G.M., (1999) Colloid Surf. B-Biointerfaces, 15, p. 3; 
Ulman, A., (1991) An Introduction to Ultrathin Organic Films: From Langmuir-Blodgett to Self-Assembled, , Academic Press Inc: San Diego, CA, USA; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., (1989) J. Am. Chem. Soc., 111, p. 321; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., (1987) J. Am. Chem. Soc., 109, p. 3559; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., (1998) J. Am. Chem. Soc., 120, p. 3464; 
Seigal, R.R., Harder, P., Dahint, R., Grunze, M., (1997) Anal. Chem., 69, p. 3321; 
L{\'o}pez, G.P., Biebuyck, H.A., Harter, R., Kumar, A., Whitesides, G.M., (1993) J. Am. Chem. Soc., 115, p. 10774; 
Prime, K., Whitesides, G.M., (1991) Science, 252, p. 1164; 
Chinn, J.A., Horbett, T.A., Ratner, B.D., (1991) Thromb. Haemost., 65, p. 608; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Silva, M.F., (1999) IEEE Trans. Magn., 35, p. 4361; 
Pale-Grosdemange, C., Simon, E.S., Prime, K.L., Whitesides, G.M., (1991) J. Am. Chem. Soc., 113, p. 12; 
Castner, D.G., Hinds, K., Grainger, D.W., (1996) Langmuir, 12, p. 5083; 
Lin, J.C., Chuang, W.H., (2000) J. Biomed. Mater. Res., 51, p. 413; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., (2001) J. Biomed. Mater. Res., 55, p. 45; 
(1993) Iodine-125, a Guide to Radioiodination Techniques - Amersham Life Science, p. 64. , Amersham International plc, Amersham Place, Little Chalfont, Bucks. HP7 9NA, England; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of Biocompatibility Tests, p. 183. , edited by D. F. Williams (Harcover, CRC Pr); 
Du, Y.J., Cornelius, R.M., Brash, J.L., (2000) Colloid Surf. B-Biointerfaces, 17, p. 59; 
Beamson, G., Briggs, D., (1992) High Resolution XPS of Organic Polymers - The Scienta ESCA300 Database, , John Wiley \& Sons Ltd, Chichester, England; 
htpp://www.lasurface.com/Tarlov, M.J., Burgess Jr., D.R.F., Gillen, G., (1993) J. Am. Chem. Soc., 115, p. 5305; 
Tegoulia, V.A., Cooper, S.L., (2000) J. Biom. Mater. Res., 50, p. 291; 
Ishida, T., Tsuneda, S., Nishida, N., Hara, M., Sasabe, H., Knoll, W., (1997) Langmuir, 13, p. 4638; 
Silver, J.H., Hergenrother, R.W., Lin, J.C., Lim, F., Lin, H.B., Okada, T., Chaudhury, M.K., Cooper, S.L., (1995) J. Biom. Mater. Res., 29, p. 535; 
Smith, T., (1980) J. Colloid. Interf. Sci., 75, p. 51; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., (1997) Langmuir, 13, p. 3404; 
Silin, V., Weetall, H., Vanderah, D., (1997) J. Colloid. Interf. Sci., 185, p. 94; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., (2003) J. Biom. Mater. Res., 67 A, p. 158; 
Schlereth, D.D., (1997) Sens. Actuator B-Chem., 43, p. 78; 
Mrksich, M., Grunwell, J.R., Whitesides, G.M., (1995) J. Am. Chem. Soc., 117, p. 12009; 
Hodneland, C.D., Lee, Y.S., Min, D.H., Mrksich, M., (2002) PNAS, 99, p. 5048; 
Malmsten, M., Lassen, B., (1994) J. Colloid. Interf. Sci., 166, p. 490; 
Feng, L., Andrade, J.D., (1994) Biomaterials, 15, p. 323; 
Bertilsson, L., Butt, H.J., Nelles, G., Schlereth, D.D., (1997) Biosens. Bioelectron., 12, p. 839
},
	month = {2003///},
	pages = {945 - 954},
	abstract = {Self-assembled monolayers can be tailored with specific ligands to a certain protein and at the same time prevent the non-specific adsorption of other proteins. Cibacron Blue F3G-A (CB-thiol) was successfully immobilized onto tetra(ethylene glycol)-terminated alkanethiol (CB-thiol). The affinity of human serum albumin (HSA) to immobilized Cibacron Blue F3G-A was studied using mixed thiolate self-assembled monolayers on gold with different n-alkyl chain lengths and functional terminal groups (CH                        3-; OH- and tetra(ethylene glycol)). Surfaces were characterized using X-ray photoelectron spectroscopy and water contact angle measurements. Albumin adsorption and exchangeability of the adsorbed albumin molecules with other albumin molecules in solution were evaluated using 
                        125I-radiolabeled HSA. Competitive adsorption between albumin and fibrinogen to the different self-assembled monolayers (SAMs) was also investigated. Results showed that the incorporation of CB-thiol on the monolayers does not increase the HSA adsorption and reversibility on the SAMs. However, although specific adsorption of HSA to the immobilized Cibacron Blue F3G-A was not demonstrated, the presence of CB-thiol decreases the affinity of fibrinogen to the OH-terminated SAMs. {\textcopyright} 2003 Kluwer Academic Publishers.
},
	keywords = {Adsorption, Albumin, aqueous solution, article, Cell immobilization, cibacron blue f3ga, Contact angle, controlled study, Drug products, Ethylene Glycol, fibrinogen, functional group, Glycols, gold, human, human serum albumin, iodine 125, isotope labeling, ligand, macrogol, molecule, priority journal, protein assembly, protein binding, protein immobilization, protein structure, Proteins, radioassay, Self assembly, surface property, synthesis, tetra(ethylene glycol) undecanethiol, thiol, thiol derivative, unclassified drug, Water, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0344629191\&partnerID=40\&md5=6ebb3020ea2ade3febc3906ce0672cc1},
	author = {Martins, Ma.C.L. and Naeemi, E. and Ratner, B.D. and Barbosa, M.A.}
}
@article {2576,
	title = {Miniaturization and application of the MTT assay to evaluate metabolic activity of protozoa in the presence of toxicants},
	journal = {Journal of Basic MicrobiologyJ. Basic Microbiol.},
	volume = {39},
	year = {1999},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JBMIEdoi: 10.1002/(SICI)1521-4028(199905)39:2<103::AID-JOBM103>3.0.CO;2-DPubMed ID: 10335603Language of Original Document: EnglishCorrespondence Address: Lima, N.; Centro de Engenharia Biologica, Universidade do Minho, 4700 Braga, Portugal; email: nelson@iec.uminho.ptChemicals/CAS: Copper, 7440-50-8; Cycloheximide, 66-81-9; Octoxynol, 9002-93-1; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1; Zinc, 7440-66-6References: Berridge, M.V., Tan, S., Characterization of the cellular reduction of using 3-[4,5-dimethyl-thiazol-2-y1]-2,5-diphenyl-tetrazolium bromide-MTT: Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction (1993) Arch. Biochem. Biophysics, 303, pp. 474-482;Berridge, M.V., Tan, S., McCoy, K.D., Wang, R., The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts (1996) Biochemica, 4, pp. 15-20; 
Carvalho, G.S., Castanheira, M., Diogo, I., Abreu, A.M., Sousa, J.P., Loon, J.A., Van Blitterswijk, C.A., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1996) J. Mat. Science: Mat. in Medicine, 7, pp. 77-83; 
Ciappeti, G., Cenni, E., Pratelli, L., Pizzoferrato, A., In vitro evaluation of cell/biomaterial interaction by MTT assay (1993) Biomaterials, 14, pp. 359-364; 
Colgan, D.J., Spectrophotometric quantitation of DNA on blots after ethanol-solubilization of the MTT-formazan from anti-digoxigenin-based detection of nucleic acids (1993) Appl. Theor. Electrophoresis, 3, pp. 219-222; 
Cote, J., Kamen, A.A., Andre, G., Protein-free culture medium improvement. Testing additives and their interactive effects in 96-well plates (1993) Appl. Microb. Biotechnol., 39, pp. 577-584; 
Dias, N., Lima, N., Carvalho, G.S., Mota, M., Miniaturiza{\c c}{\~a}o do ensaio do MTT para avalia{\c c}{\~a}o da actividade metab{\'o}lica dos protozo{\'a}rios (1997) Congresso Nacional de Microbiologia-MICRO{\textquoteright} 97, , Tomar; 
Hanel, T., Gareis, M., Kollarczik, B., Citotoxicity of mycotoxins evaluated by the MTT-cell culture Assay (1994) Mycopathologia, 128, pp. 167-174; 
Lemieux, P., Michaud, M., Pag{\'e}, M., A new formazan amplified clonogenic assay for cytotoxicity testing (1993) Biotechnol. Techniques, 7, pp. 597-602; 
Martin, A., Clynes, C., Comparison of 5 microplate assays for in vitro cytotoxicity testing and cell proliferation assays (1993) Cytotechnology, 11, pp. 49-58; 
Mossman, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J. Immunol. Methods, 65, pp. 55-63; 
Roberts, Ch.T., Orias, E., A cycloheximide-resistant mutant of Tetrahymena pyriformis (1973) Exp. Cell Res., 81, pp. 312-316; 
Sasaki, C.Y., Passaniti, A., Identification of anti-invasive but noncytotoxic chemotherapeutic agents using the tetrazolium dye MTT to quantitative viable cells in matrigel (1998) Bio-techniques, 24, pp. 1038-1043; 
Stowe, R.P., Koenig, D.W., Mishra, S.K., Pierson, D.L., Non destructive and continuous spectrophotometric measurement of cell respiration using a tetrazolium-formazan microemulsion (1995) J. Microbiol. Methods, 22, pp. 283-292; 
Wilson, A.P., Citotoxicity and viability assays (1995) Animal Cell Culture - A Practical Approach. 2nd Ed., pp. 277-289. , (R. I. FRESHNEY, Editor). Oxford University Press, Oxford
},
	month = {1999///},
	pages = {103 - 108},
	abstract = {This paper describes a critical evaluation of a miniaturised colorimetric assay, using MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) reduction, applied to protozoan viability testing. The toxic substances used were copper, zinc, Triton X-100 (a membrane surfactant) and cycloheximide (an inhibitor of the protein synthesis). The viability assay of the ciliate protozoan Tetrahymena pyriformis was optimised in terms of MTT concentration and incubation time. Since protozoa are non adherent cells the MTT assay was modified in order to maintain the medium in the well. MTT proved to be effective in the measurement of Tetrahymena pyriformis viability. Four hours of MTT incubation followed by 30 minutes of incubation with DMSO were found to be the best incubation times for optical density reading. Furthermore, 10 mg/ml of MTT solution was the concentration that gave higher values of optical densities with minor medium interference.},
	keywords = {Animals, Ciliophora, Colorimetry, Copper, Cycloheximide, MTT assay, Octoxynol, Protozoa, Tetrahymena pyriformis, Tetrazolium Salts, Thiazoles, Zinc},
	isbn = {0233111X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032910642\&partnerID=40\&md5=81e222339d4ee5c21619858027047e83},
	author = {Dias, N. and Nicolau, A. and Carvalho, G.S. and Mota, M. and Lima, N.}
}
