



@article {3390,
	title = {Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach},
	journal = {Industrial Crops and Products},
	volume = {63},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {167-174},
	isbn = {0926-6690},
	url = {<Go to ISI>://WOS:000347499000025},
	author = {Rodrigues, F. and Pereira, C. and Pimentel, F. B. and Alves, R. C. and Ferreira, M. and Sarmento, B. and Helena Amaral, M. and Oliveira, M. Beatriz P. P.}
}
@article {3391,
	title = {Coffee silverskin: A possible valuable cosmetic ingredient},
	journal = {Pharmaceutical Biology},
	volume = {53},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {386-394},
	isbn = {1388-0209},
	url = {<Go to ISI>://WOS:000348831200011},
	author = {Rodrigues, Francisca and Palmeira-de-Oliveira, Ana and das Neves, Jose and Sarmento, Bruno and Helena Amaral, M. and Oliveira, M. Beatriz P. P.}
}
@article {3385,
	title = {Combined C4d and CD3 immunostaining predicts immunoglobulin (Ig)A nephropathy progression},
	journal = {Clinical and Experimental Immunology},
	volume = {179},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {354-361},
	isbn = {0009-9104},
	url = {<Go to ISI>://WOS:000347233800020},
	author = {Faria, B. and Henriques, C. and Matos, A. C. and Daha, M. R. and Pestana, M. and Seelen, M.}
}
@article {3383,
	title = {Cortical and trabecular bone in pediatric end-stage kidney disease},
	journal = {Pediatric Nephrology},
	volume = {30},
	year = {2015},
	month = {2015},
	pages = {497-502},
	isbn = {0931-041X},
	url = {<Go to ISI>://CCC:000349440700016},
	author = {Carvalho, Catarina G. and Pereira, Renata C. and Gales, Barbara and Salusky, Isidro B. and Wesseling-Perry, Katherine}
}
@article {3387,
	title = {Finite element analysis of pectus carinatum surgical correction via a minimally invasive approach},
	journal = {Computer Methods in Biomechanics and Biomedical Engineering},
	volume = {18},
	year = {2015},
	note = {Times Cited: 0Neves, Sara/A-5993-2015
Neves, Sara/0000-0001-6154-2821
},
	month = {2015},
	pages = {711-720},
	isbn = {1025-5842},
	url = {<Go to ISI>://WOS:000345141700003},
	author = {Neves, Sara C. and Pinho, A. C. M. and Fonseca, Jaime C. and Rodrigues, Nuno F. and Henriques-Coelho, Tiago and Correia-Pinto, Jorge and Vilaca, Joao L.}
}
@article {3388,
	title = {The interactions between municipal socioeconomic status and age on hip fracture risk},
	journal = {Osteoporosis International},
	volume = {26},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {489-498},
	isbn = {0937-941X},
	url = {<Go to ISI>://WOS:000349018700008},
	author = {Oliveira, C.M. and Economou, T. and Bailey, T. and Mendona, D. and Pina, M.F.}
}
@article {3389,
	title = {The role of the surface on microglia function: implications for central nervous system tissue engineering},
	journal = {Journal of the Royal Society, Interface / the Royal Society},
	volume = {12},
	year = {2015},
	month = {2015},
	url = {<Go to ISI>://MEDLINE:25540243},
	author = {Pires, Liliana R. and Rocha, Daniela N. and Ambrosio, Luigi and Pego, Ana Paula}
}
@article {3263,
	title = {Age-period-cohort effects in the incidence of hip fractures: Political and economic events are coincident with changes in risk},
	journal = {Osteoporosis International},
	volume = {25},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {711 - 720},
	abstract = {Summary: An age-period cohort model was fitted to analyse time effects on hip fracture incidence rates by sex (Portugal, 2000-2008). Rates increased exponentially with age (age effect). Incidence rates decreased after 2004 for women and were random for men (period effect). New but comprehensive fluctuations in risk were coincident with major political/economic changes (cohort effect). Introduction: Healthcare improvements have allowed prevention but have also increased life expectancy, resulting in more people being at risk. Our aim was to analyse the separate effects of age, period and cohort on incidence rates by sex in Portugal, 2000-2008. Methods: From the National Hospital Discharge Register, we selected admissions (aged >=49 years) with hip fractures (ICD9-CM, codes 820.x) caused by low/moderate trauma (falls from standing height or less), readmissions and bone cancer cases. We calculated person-years at risk using population data from Statistics Portugal. To identify period and cohort effects for all ages, we used an age-period-cohort model (1-year intervals) followed by generalised additive models with a negative binomial distribution of the observed incidence rates of hip fractures. Results: There were 77,083 hospital admissions (77.4\% women). Incidence rates increased exponentially with age for both sexes (age effect). Incidence rates fell after 2004 for women and were random for men (period effect). There was a general cohort effect similar in both sexes; risk of hip fracture altered from an increasing trend for those born before 1930 to a decreasing trend following that year. Risk alterations (not statistically significant) coincident with major political and economic change in the history of Portugal were observed around birth cohorts 1920 (stable-increasing), 1940 (decreasing-increasing) and 1950 (increasing-decreasing only among women). Conclusions: Hip fracture risk was higher for those born during major economically/politically unstable periods. Although bone quality reflects lifetime exposure, conditions at birthmay determine future risk for hip fractures. {\textcopyright} International Osteoporosis Foundation and National Osteoporosis Foundation 2013.},
	keywords = {Age-period-cohort, Hip fractures, Osteoporosis, Population-based study, Time trend},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84899095788\&partnerID=40\&md5=8a5587d6365266284e4fbc4e2be28e7d},
	author = {Alves, S.M. and Castiglione, D. and Oliveira, C.M. and de Sousa, B. and Pina, M.F.}
}
@article {3237,
	title = {Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand},
	journal = {Journal of the Royal Society, Interface / the Royal Society},
	volume = {11},
	year = {2014},
	month = {2014},
	url = {<Go to ISI>://MEDLINE:25320070},
	author = {Parreira, P. and Shi, Q. and Magalhaes, A. and Reis, C.A. and Bugaytsova, J. and Boren, T. and Leckband, D. and Martins, M.C.L.}
}
@article {3415,
	title = {Bioactivity of surface tethered Osteogenic Growth Peptide motifs},
	journal = {Medchemcomm},
	volume = {5},
	year = {2014},
	note = {Times Cited: 0},
	month = {2014},
	pages = {899-903},
	isbn = {2040-2503},
	url = {<Go to ISI>://WOS:000338431500007},
	author = {Panseri, Silvia and Russo, Laura and Montesi, Monica and Taraballi, Francesca and Cunha, Carla and Marcacci, Maurilio and Cipolla, Laura}
}
@article {3284,
	title = {Building and repairing the heart: What can we learn from embryonic development?},
	journal = {BioMed Research International},
	volume = {2014},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Mammalian heart formation is a complex morphogenetic event that depends on the correct temporal and spatial contribution of distinct cell sources. During cardiac formation, cellular specification, differentiation, and rearrangement are tightly regulated by an intricate signaling network. Over the last years, many aspects of this network have been uncovered not only due to advances in cardiac development comprehension but also due to the use of embryonic stem cells (ESCs) in vitro model system. Additionally, several of these pathways have been shown to be functional or reactivated in the setting of cardiac disease. Knowledge withdrawn from studying heart development, ESCs differentiation, and cardiac pathophysiology may be helpful to envisage new strategies for improved cardiac repair/regeneration. In this review, we provide a comparative synopsis of the major signaling pathways required for cardiac lineage commitment in the embryo and murine ESCs. The involvement and possible reactivation of these pathways following heart injury and their role in tissue recovery will also be discussed. {\textcopyright} 2014 Ana G. Freire et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84900022530\&partnerID=40\&md5=892677562f6828017ad5a18fe9e97e83},
	author = {Freire, AG and Resende, TP and Pinto-Do-{\'O}, P.}
}
@article {3325,
	title = {Collagen surface modified poly(ε-caprolactone) scaffolds with improved hydrophilicity and cell adhesion properties},
	journal = {Materials Letters},
	volume = {134},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {263 - 267},
	abstract = {Poly(ε-caprolactone) (PCL)/collagen scaffolds were produced using a four-step procedure comprising (i) the fabrication of PCL scaffolds through an additive bioextrusion system, (ii) the PCL surface modification using a plasma treatment, (iii) the acrylic acid (AAc) grafting by ultraviolet (UV) polymerization, and (iv) the immobilization of collagen into the scaffold surface through the carbodiimide chemistry. This procedure allowed the quick fabrication of highly porous scaffolds with interconnected pores, well-defined internal architecture and improved cell adhesion properties. The immobilization of collagen into the PCL surface led to a significant enhancement in the hydrophilicity, while the biological tests showed a further improvement in the adhesion of fibroblast cells. These results indicate that the collagen surface modification is an effective strategy to render the hydrophobic nature of PCL and overcome the limited cell interaction of 3D PCL scaffolds. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {BioExtruder, Collagen, poly(ε-caprolactone), Scaffold, Surface modification},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905669651\&partnerID=40\&md5=ebb38981b89fc93e5599a7592a37e134},
	author = {Sousa, I. and Mendes, A. and Pereira, R.F. and B{\'a}rtolo, P.J.}
}
@article {3312,
	title = {Development and evaluation of an algorithm for computer analysis of maternal heart rate during labor},
	journal = {Computers in Biology and Medicine},
	volume = {49},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {30 - 35},
	abstract = {Background: Maternal heart rate (MHR) recordings are morphologically similar and sometimes coincident with fetal heart rate (FHR) recordings and may be useful for maternal-fetal monitoring if appropriately interpreted. However, similarly to FHR, visual interpretation of MHR features may be poorly reproducible. Methods: A computer algorithm for on-line MHR analysis was developed based on a previously existing version for FHR analysis. Inter-observer and computer-observer agreement and reliability were assessed in 40 one-hour recordings obtained from 20 women during the last 2. h of labor. Agreement and reliability were evaluated for the detection of basal MHR, long-term variability (LTV), accelerations and decelerations, using proportions of agreement (PA) and Kappa statistic (K), with 95\% confidence intervals (95\% CI). Changes in MHR characteristics between the first and the second hour of the tracings were also evaluated. Results: There was a statistically significant inter-observer and computer-observer agreement and reliability in estimation of basal MHR, accelerations, decelerations and LTV, with PA values ranging from 0.72 (95\% CI: 0.62-0.79) to 1.00 (95\% CI: 0.99-1.00), and K values ranging from 0.44 (95\% CI: 0.28-0.60) to 0.89 (95\% CI: 0.82-0.96). Moreover, basal MHR, number of accelerations and LTV were significantly higher in the last hour of labor, when compared to the initial hour. Discussion: The developed algorithm for on-line computer analysis of MHR recordings provided good to excellent computer-observer agreement and reliability. Moreover, it allowed an objective detection of MHR changes associated with labor progression, providing more information about the interpretation of maternal-fetal monitoring during labor. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {Agreement, Cardiotocography, computer analysis, Fetal heart rate, Maternal heart rate, reliability},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84897934596\&partnerID=40\&md5=95b314c6374a68fedb313594f59fdcce},
	author = {Pinto, P. and Bernardes, J. and Costa-Santos, C. and Amorim-Costa, C. and Silva, M. and Ayres-de-Campos, D.}
}
@article {3161,
	title = {Dissecting the signaling pathways associated with the oncogenic activity of MLK3 P252H mutation},
	journal = {Bmc Cancer},
	volume = {14},
	year = {2014},
	note = {Times Cited: 0},
	month = {2014},
	isbn = {1471-2407},
	url = {<Go to ISI>://WOS:000333421700002},
	author = {Velho, Sergia and Pinto, Ana and Licastro, Danilo and Oliveira, Maria Jose and Sousa, Filipa and Stupka, Elia and Seruca, Raquel}
}
@article {3324,
	title = {DNAJB4 molecular chaperone distinguishes WT from mutant E-cadherin, determining their fate in vitro and in vivo},
	journal = {Human Molecular Genetics},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2094 - 2105},
	abstract = {E-cadherin (Ecad) is a well-known invasion suppressor and its loss of expression is common in invasive carcinomas. Germline Ecad mutations are the only known genetic cause of hereditary diffuse gastric cancer (HDGC), demonstrating the causative role of Ecad impairment in gastric cancer. HDGC-associated Ecad missense mutations can lead to folding defects and premature proteasome-dependent endoplasmic reticulum-associated degradation (ERAD), but the molecular determinants for this fate were unidentified. Using a Drosophilabased genetic screen, we found that Drosophila DnaJ-1 interacts with wild type (WT) and mutant human Ecad in vivo. DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4), the human homolog of DnaJ-1, influences Ecad localization and stability even in the absence of Ecad endogenous promoter, suggesting a post-transcriptional level of regulation. Increased expression of DNAJB4 leads to stabilization of WT Ecad in the plasma membrane, while it induces premature degradation of unfolded HDGC mutants in the proteasome. The interaction between DNAJB4 and Ecad is direct, and is increased in the context of the unfolded mutant E757K, especially when proteasome degradation is inhibited, suggesting that DNAJB4 is a molecular mediator of ERAD. Post-translational regulation of native Ecad by DNAJB4 molecular chaperone is sufficient to influence cell adhesion in vitro. Using a chick embryo chorioallantoic membrane assay with gastric cancer derived cells, we demonstrate that DNAJB4 stimulates the anti-invasive function of WT Ecad in vivo. Additionally, the expression of DNAJB4 and Ecad is concomitantly decreased in human gastric carcinomas. Altogether, we demonstrate that DNAJB4 is a sensor of Ecad structural features that might contribute to gastric cancer progression. {\textcopyright} The Author 2013. Published by Oxford University Press. All rights reserved.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84897831766\&partnerID=40\&md5=c7651a5372b8a46de1f028888590b3f3},
	author = {Sim{\~o}es-Correia, J. and Silva, D.I. and Melo, S. and Figueiredo, J. and Caldeira, J. and Pinto, M.T. and Gir{\~a}o, H. and Pereira, P. and Seruca, R.}
}
@article {3278,
	title = {Dysregulation of T cell receptor N-glycosylation: A molecular mechanism involved in ulcerative colitis},
	journal = {Human Molecular Genetics},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2416 - 2427},
	abstract = {The incidence of inflammatory bowel disease is increasing worldwide and the underlying molecular mechanisms are far from being fully elucidated. Herein, we evaluated the role of N-glycosylation dysregulation in T cells as a key mechanism in the ulcerative colitis (UC) pathogenesis. The evaluation of the branched N-glycosylation levelsandprofile of intestinalTcell receptor (TCR)wereassessedin colonic biopsies fromUCpatientsand healthy controls. Expression alterations of the glycosyltransferase gene MGAT5 were also evaluated. We demonstrated thatUCpatients exhibit a dysregulation ofTCRbranchedN-glycosylationonlamina propriaTlymphocytes. Patients with severe UC showed the most pronounced defect on N-glycan branching in T cells. Moreover, UC patients showed a significant reduction of MGAT5 gene transcription in T lymphocytes. In this study, we disclose for the first time that a deficiency in branched N-glycosylation on TCR due to a reduced MGAT5 gene expression is a new molecular mechanism underlying UC pathogenesis, being a potential novel biomarker with promising clinical and therapeutic applications. {\textcopyright} The Author 2013. Published by Oxford University Press. All rights reserved.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84897565140\&partnerID=40\&md5=4ee6b5b3f85e16eba322ceb8e0d69dd3},
	author = {Dias, A.M. and Dourado, J. and Lago, P. and Cabral, J. and Marcos-Pinto, R. and Salgueiro, P. and Almeida, C.R. and Carvalho, S. and Fonseca, S. and Lima, M. and Vilanova, M. and Dinis-Ribeiro, M. and Reis, C.A. and Pinho, S.S.}
}
@article {3311,
	title = {The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {8144 - 8153},
	abstract = {Intervertebral disc (IVD) degeneration is the leading cause of low back pain and disability in the active population. Transplantation of mesenchymal stem cells (MSCs) in a hydrogel carrier can induce regenerative effects in degenerated IVDs. Moreover, it was found that degenerative discs release chemoattractants effective in MSC recruitment. Based on these findings, we hypothesized that an injectable hydrogel that can enhance the number of migrated MSCs in the IVD and provide a suitable matrix for their survival and differentiation would be ideal. The purpose of this study was to evaluate the potential of a thermoreversible hyaluronan-poly(N-isopropylacrylamide) (HAP) hydrogel as chemoattractant delivery system to recruit human MSCs in degenerative IVDs. The results demonstrate that HAP hydrogels containing stromal cell derived factor-1 (SDF-1) significantly increased the number of MSCs migrating into nucleotomized discs compared with discs treated with only HAP or SDF-1 in solution. HAP hydrogels releasing SDF-1 enhanced both the number of recruited cells and their migration distance in the IVD tissue. Furthermore, this phenomenon was dependent on MSC donor age. In conclusion, HAP SDF-1 is effective for the recruitment of stem cells in the IVD, thus opening new possibilities for the development of regenerative therapies based on endogenous cell migration. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {Intervertebral disc, Mesenchymal Stem Cells, Recruitment, Stromal cell derived factor-1, Thermoreversible hyaluronan hydrogel},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903746142\&partnerID=40\&md5=1366ab2e3399d0a363aab2e356bbdf96},
	author = {Pereira, C.L. and Gon{\c c}alves, R.M. and Peroglio, M. and Pattappa, G. and D{\textquoteright}Este, M. and Eglin, D. and Barbosa, M.A. and Alini, M. and Grad, S.}
}
@article {3406,
	title = {Endoplasmic Reticulum Degradation-Enhancing alpha-Mannosidase-like Protein 1 Targets Misfolded HLA-B27 Dimers for Endoplasmic Reticulum-Associated Degradation},
	journal = {Arthritis \& Rheumatology},
	volume = {66},
	year = {2014},
	month = {2014},
	pages = {2976-2988},
	url = {<Go to ISI>://WOS:000344349500008},
	author = {Guiliano, David B. and Fussell, Helen and Lenart, Izabela and Tsao, Edward and Nesbeth, Darren and Fletcher, Adam J. and Campbell, Elaine C. and Yousaf, Nasim and Williams, Sarah and Santos, Susana and Cameron, Amy and Towers, Greg J. and Kellam, Paul and Hebert, Daniel N. and Gould, Keith G. and Powis, Simon J. and Antoniou, Antony N.}
}
@article {3287,
	title = {External uterine contractions signal analysis in relation to labor progression and dystocia},
	journal = {IFMBE Proceedings},
	volume = {41},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {555 - 558},
	abstract = {Labor dystocia is a major cause of operative delivery, which is associated with increased risks for both mother and fetus. We assessed linear and non-linear dynamics of external uterine contraction signals, in relation to labor progression and dystocia. Linear time domain, spectral and entropy methods were used to analyze external uterine contraction recordings obtained during the last two hours of labor, in 28 cases with normal and 27 cases with operative deliveries (forceps, vacuum or caesarean). Progression of labor was associated with a statistically significant increase in most linear time domain and spectral indices, both in normal and operative deliveries, whereas most entropy indices increased in normal deliveries, but did not change in operative deliveries. On the other hand, when compared with normal births, operative deliveries were associated with significantly increased entropy indices before the last hour of labor and significantly decreased (a probably associated) sympatho-vagal balance in the last hour of labor. Linear and non-linear analysis of external uterine contraction recordings may provide useful physiopathological and clinical information on the progression of labor and the diagnosis of dystocia. {\textcopyright} Springer International Publishing Switzerland 2014.},
	keywords = {Cardiotocography, Dystocia, entropy, External uterine contractions, Spectral analysis},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84891293956\&partnerID=40\&md5=2d53a97f6000b704bf67435b61662c09},
	author = {Gon{\c c}alves, H. and Pinto, P. and Ayres-de-Campos, D. and Bernardes, J.}
}
@article {3328,
	title = {Ganciclovir-resistant cytomegalovirus infection in renal transplantation},
	journal = {Clinical Kidney Journal},
	volume = {7},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {210 - 213},
	keywords = {cytomegalovirus infection, ganciclovir resistance},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84897409543\&partnerID=40\&md5=af3bc36958fba967d5c9f98953d03572},
	author = {Vaz, R. and Barros, F. and Tavares, I. and Bustorff, M. and Ferreira, I. and Pestana, M.}
}
@article {3303,
	title = {Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure},
	journal = {ACS Nano},
	volume = {8},
	year = {2014},
	note = {Cited By (since 1996):4Export Date: 21 November 2014},
	month = {2014},
	pages = {2033 - 2047},
	abstract = {Stem cell responsiveness to extracellular matrix (ECM) composition and mechanical cues has been the subject of a number of investigations so far, yet the molecular mechanisms underlying stem cell mechano-biology still need full clarification. Here we demonstrate that the paralog proteins YAP and TAZ exert a crucial role in adult cardiac progenitor cell mechano-sensing and fate decision. Cardiac progenitors respond to dynamic modifications in substrate rigidity and nanopattern by promptly changing YAP/TAZ intracellular localization. We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design. {\textcopyright} 2014 American Chemical Society.},
	keywords = {adult cardiac progenitor cell, cardiac differentiation, mechano- transduction, substrate nanotopography, YAP/TAZ},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896911335\&partnerID=40\&md5=c15ea687a167227f6d91e71dcc812590},
	author = {Mosqueira, D. and Pagliari, S. and Uto, K and Ebara, M and Romanazzo, S. and Escobedo-Lucea, C and Nakanishi, J and Taniguchi, A and Franzese, O and Di Nardo, P. and Goumans, MJ and Traversa, E and Pinto-Do-{\'O}, P. and Aoyagi, T. and Forte, G.}
}
@article {3305,
	title = {Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms},
	journal = {Stem Cell Research and Therapy},
	volume = {5},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	abstract = {Introduction. Among the plethora of cells under investigation to restore a functional myocardium, mesenchymal stromal cells (MSCs) have been granted considerable interest. However, whereas the beneficial effects of bone marrow MSCs (BM-MSCs) in the context of the diseased heart are widely reported, data are still scarce on MSCs from the umbilical cord matrix (UCM-MSCs). Herein we report on the effect of UCM-MSC transplantation to the infarcted murine heart, seconded by the dissection of the molecular mechanisms at play. Methods. Human umbilical cord tissue-derived MSCs (UCX{\textregistered}), obtained by using a proprietary technology developed by ECBio, were delivered via intramyocardial injection to C57BL/6 females subjected to permanent ligation of the left descending coronary artery. Moreover, medium produced by cultured UCX{\textregistered} preconditioned under normoxia (CM) or hypoxia (CMH) was collected for subsequent in vitro assays. Results: Evaluation of the effects upon intramyocardial transplantation shows that UCX{\textregistered} preserved cardiac function and attenuated cardiac remodeling subsequent to myocardial infarction (MI). UCX{\textregistered} further led to increased capillary density and decreased apoptosis in the injured tissue. In vitro, UCX{\textregistered}-conditioned medium displayed (a) proangiogenic activity by promoting the formation of capillary-like structures by human umbilical vein endothelial cells (HUVECs), and (b) antiapoptotic activity in HL-1 cardiomyocytes subjected to hypoxia. Moreover, in adult murine cardiac Sca- 1{\textsection}ssup{\textsection}+{\textsection}esup{\textsection} progenitor cells (CPCs), conditioned medium enhanced mitogenic activity while activating a gene program characteristic of cardiomyogenic differentiation. Conclusions: UCX{\textregistered} preserve cardiac function after intramyocardial transplantation in a MI murine model. The cardioprotective effects of UCX{\textregistered} were attributed to paracrine mechanisms that appear to enhance angiogenesis, limit the extent of the apoptosis, augment proliferation, and activate a pool of resident CPCs. Overall, these results suggest that UCX{\textregistered} should be considered an alternative cell source when designing new therapeutic approaches to treat MI. {\textcopyright} 2014 Nascimento et al.; licensee BioMed Central Ltd.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896713037\&partnerID=40\&md5=22fdca81d834ec78c8196552c3d250ae},
	author = {Nascimento, D.S. and Mosqueira, D. and Sousa, L.M. and Teixeira, M. and Filipe, M. and Resende, TP and Ara{\'u}jo, A.F. and Valente, M. and Almeida, J. and Martins, J.P. and Santos, J.M. and B{\'a}rcia, R.N. and Cruz, P. and Cruz, H. and Pinto-Do-{\'O}, P.}
}
@booklet {3260,
	title = {Impact of 3-D printed PLA- and chitosan-based scaffolds on human monocyte/macrophage responses: Unraveling the effect of 3-D structures on inflammation},
	howpublished = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {613 - 622},
	abstract = {Recent studies have pointed towards a decisive role of inflammation in triggering tissue repair and regeneration, while at the same time it is accepted that an exacerbated inflammatory response may lead to rejection of an implant. Within this context, understanding and having the capacity to regulate the inflammatory response elicited by 3-D scaffolds aimed for tissue regeneration is crucial. This work reports on the analysis of the cytokine profile of human monocytes/macrophages in contact with biodegradable 3-D scaffolds with different surface properties, architecture and controlled pore geometry, fabricated by 3-D printing technology. Fabrication processes were optimized to create four different 3-D platforms based on polylactic acid (PLA), PLA/calcium phosphate glass or chitosan. Cytokine secretion and cell morphology of human peripheral blood monocytes allowed to differentiate on the different matrices were analyzed. While all scaffolds supported monocyte/macrophage adhesion and stimulated cytokine production, striking differences between PLA-based and chitosan scaffolds were found, with chitosan eliciting increased secretion of tumor necrosis factor (TNF)-α, while PLA-based scaffolds induced higher production of interleukin (IL)-6, IL-12/23 and IL-10. Even though the material itself induced the biggest differences, the scaffold geometry also impacted on TNF-α and IL-12/23 production, with chitosan scaffolds having larger pores and wider angles leading to a higher secretion of these pro-inflammatory cytokines. These findings strengthen the appropriateness of these 3-D platforms to study modulation of macrophage responses by specific parameters (chemistry, topography, scaffold architecture). {\textcopyright} 2013 Acta Materialia Inc.},
	keywords = {3-D scaffolds, Human macrophages, Inflammation, Rapid prototyping, Tissue engineering},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896505907\&partnerID=40\&md5=948f87b684be751bc6eb444e28ce6025},
	author = {Almeida, C.R. and Serra, T. and Oliveira, M.I. and Planell, J.A. and Barbosa, M.A. and Navarro, M.}
}
@article {3290,
	title = {In vitro analysis of the antibacterial effect of nanohydroxyapatite-ZnO composites},
	journal = {Journal of Biomedical Materials Research - Part A},
	volume = {102},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {3726 - 3733},
	abstract = {Hydroxyapatite (HA) is a biocompatible and bioactive synthetic material for biomedical applications as it binds to bone and enhances bone tissue formation. Particularly, nanophased HA can mimic the dimensions of constituent components of natural tissues; can modulate enhanced osteoblast adhesion and resorption with long-term functionality of tissue engineered implants. However, HA does not inhibit bacteria from adhering onto its surface, and this has implications in the bone healing process required for patient recovery, since infection can lead to the implant failure. In the present work a composite that combines the favorable biological characteristics of nanohydroxyapatite (nanoHA) and, simultaneously, possesses antimicrobial activity as expressed by ZnO was synthesized. To determine whether the size of ZnO particles was playing an important role in inhibiting bacterial growth, ZnO particle of different sizes (from the microscale down to the nanoscale) and concentration were incorporated into nanoHA and tested. The composite samples were characterized by SEM, FT-IR, XRD, XPS and zeta potential. The antibacterial activity of the composites was investigated, as well as the biofilm formation, using both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) microorganisms. The characterization revealed that ZnO particles were dispersed homogeneously within the nanoHA matrix. The composites antibacterial activity increased with decreasing ZnO particle size and increasing concentration. Biofilm formation tests revealed that the nanoHA-ZnO composites exhibit a strong effect against the common pathogens S. aureus and E. coli. {\textcopyright} 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3726-3733, 2014. {\textcopyright} 2013 Wiley Periodicals, Inc.},
	keywords = {Antibacterial, biofilm, Composite, Nanohydroxyapatite, zinc oxide},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84906789309\&partnerID=40\&md5=1f841e9eed15abe9adeb1a140e97a20a},
	author = {Grenho, L. and Jorge Monteiro, F. and Pia Ferraz, M.}
}
@article {3279,
	title = {In vitro intestinal absorption of amino acid mixtures extracted from codfish (Gadus morhua L.) salting wastewater},
	journal = {International Journal of Food Science and Technology},
	volume = {49},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {27 - 33},
	abstract = {Summary: This study reports on the intestinal permeability of salt-containing mixtures of amino acids extracted from codfish salting wastewater. Permeability was evaluated in vitro using the Caco-2 cell line model; cell integrity during exposure to mixtures of amino acids was estimated by measuring the transepithelial electrical resistance (TEER). The effect of salt (NaCl) on the permeability and on the intestinal cell{\textquoteright}s integrity was also examined. Permeation rate (i.e. transport) was >=95\% for all amino acids except for creatine, for which it was 6\%. Values for apparent permeability coefficients, Papp \&gt; 10-5 cm s-1, for mixture with isotonic concentration of NaCl suggest that amino acids are very likely to be absorbed in humans. Mixture with a hypertonic level of NaCl exerts a cytotoxic effect in intestinal cells resulting in a loss of epithelium integrity. Results show that isotonic mixture of amino acids extracted from codfish salting wastewater could be used in food, feed, cosmetic and pharmaceutical formulations. These applications could contribute to the fish industry sustainability. {\textcopyright} 2013 Institute of Food Science and Technology.},
	keywords = {Absorption, Amino acids, Caco-2 cell, Codfish, Small intestine},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84890426132\&partnerID=40\&md5=2727df048ef513e5a6a2978cfe149092},
	author = {Ferraro, V. and Ferreira Jorge, R. and Cruz, I.B. and Antunes, F. and Sarmento, B. and Castro, P.M.L. and Pintado, M.E.}
}
@article {3302,
	title = {Injectable pectin hydrogels produced by internal gelation: PH dependence of gelling and rheological properties},
	journal = {Carbohydrate Polymers},
	volume = {103},
	year = {2014},
	note = {Cited By (since 1996):5Export Date: 21 November 2014},
	month = {2014},
	pages = {339 - 347},
	abstract = {The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery. {\textcopyright} 2013 Elsevier Ltd.},
	keywords = {Biomaterials, Internal gelation, Natural polymers, pectin, Tissue engineering},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84892575017\&partnerID=40\&md5=91c88ab2cf99ad8c279d1253e930ef01},
	author = {Moreira, H.R. and Munarin, F. and Gentilini, R. and Visai, L. and Granja, P.L. and Tanzi, M.C. and Petrini, P.}
}
@article {3270,
	title = {Macrophages stimulate gastric and colorectal cancer invasion through EGFR Y 1086, c-Src, Erk1/2 and Akt phosphorylation and smallGTPase activity},
	journal = {Oncogene},
	volume = {33},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {2123 - 2133},
	abstract = {The interactions between cancer cells and their microenvironment are crucial for malignant progression, as they modulate invasion-related activities. Tumor-associated macrophages are generally considered allies in the process of tumor progression in several types of cancer, although their role on gastric and colorectal carcinomas is still poorly understood. In this report, we studied the influence of primary human macrophages on gastric and colorectal cancer cells, considering invasion, motility/migration, proteolysis and activated intracellular signaling pathways. We demonstrated that macrophages stimulate cancer cell invasion, motility and migration, and that these effects depend on matrix metalloproteinase (MMP) activity and on the activation of epidermal growth factor receptor (EGFR) (at the residue Y 1086), PLC-γ (phospholipase C-gamma) and Gab1 (GRB2-associated binding protein-1), as evidenced by siRNA (small interference RNA) experiments. Epidermal growth factor (EGF)-immunodepletion impaired macrophage-mediated cancer cell invasion and motility, suggesting that EGF is the pro-invasive and pro-motile factor produced by macrophages. Macrophages also induced gastric and colorectal cancer cell phosphorylation of Akt, c-Src and ERK1/2, and led to an increase of RhoA and Cdc42 activity. Interestingly, whereas macrophage-mediated cancer cell c-Src and ERK1/2 phosphorylation occurred downstream EGFR activation, Akt phosphorylation seems to be a parallel event, taking place in an EGFR-independent manner. The involvement of EGF, EGFR-downstream signaling partners and MMPs in macrophage-mediated invasion provides novel insights into the molecular crosstalk established between cancer cells and macrophages, opening new perspectives for the design of new and more efficient therapeutic strategies to counteract cancer cell invasion. {\textcopyright} 2014 Macmillan Publishers Limited.},
	keywords = {cancer cell invasion, colon cancer, Gastric cancer, invasion-related signaling pathways, Macrophages, tumor microenvironment},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84899425243\&partnerID=40\&md5=beff5192ff42940c75fef8b48ebd3ae7},
	author = {Cardoso, A.P. and Pinto, M.L. and Pinto, A.T. and Oliveira, M.I. and Pinto, M.T. and Gon{\c c}alves, R. and Relvas, J.B. and Figueiredo, C. and Seruca, R. and Mantovani, A. and Mareel, M. and Barbosa, M.A. and Oliveira, M.J.}
}
@article {3257,
	title = {Magnetic Bioinspired Hybrid Nanostructured Collagen-Hydroxyapatite Scaffolds Supporting Cell Proliferation and Tuning Regenerative Process},
	journal = {Acs Applied Materials \& Interfaces},
	volume = {6},
	year = {2014},
	month = {2014},
	pages = {15697-15707},
	url = {<Go to ISI>://WOS:000342328300010},
	author = {Tampieri, Anna and Iafisco, Michele and Sandri, Monica and Panseri, Silvia and Cunha, Carla and Sprio, Simone and Savini, Elisa and Uhlarz, Marc and Herrmannsdoerfer, Thomas}
}
@article {3413,
	title = {Microfluidics co-culture systems for studying tooth innervation},
	journal = {Frontiers in Physiology},
	volume = {5},
	year = {2014},
	note = {Times Cited: 0Lamghari, Meriem/K-2159-2013
Lamghari, Meriem/0000-0002-2500-4548
},
	month = {2014},
	isbn = {1664-042X},
	url = {<Go to ISI>://WOS:000347083900001},
	author = {Pagella, Pierfrancesco and Neto, Estrela and Jimenez-Rojo, Lucia and Lamghari, Meriem and Mitsiadis, Thimios A.}
}
@article {3319,
	title = {Modified-chitosan/siRNA nanoparticles downregulate cellular CDX2 expression and cross the gastric mucus barrier},
	journal = {PLoS ONE},
	volume = {9},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Development of effective non-viral vectors is of crucial importance in the implementation of RNA interference in clinical routine. The localized delivery of siRNAs to the gastrointestinal mucosa is highly desired but faces specific problems such as the stability in gastric acidity conditions and the presence of the mucus barrier. CDX2 is a transcription factor critical for intestinal differentiation being involved in the initiation and maintenance of gastrointestinal diseases. Specifically, it is the trigger of gastric intestinal metaplasia which is a precursor lesion of gastric cancer. Its expression is also altered in colorectal cancer, where it may constitute a lineage-survival oncogene. Our main objective was to develop a nanoparticle-delivery system of siRNA targeting CDX2 using modified chitosan as a vector. CDX2 expression was assessed in gastric carcinoma cell lines and nanoparticles behaviour in gastrointestinal mucus was tested in mouse explants. We show that imidazolemodified chitosan and trimethylchitosan/siRNA nanoparticles are able to downregulate CDX2 expression and overpass the gastric mucus layer but not colonic mucus. This system might constitute a potential therapeutic approach to treat CDX2-dependent gastric lesions. {\textcopyright} 2014 Sadio et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903390267\&partnerID=40\&md5=61e637790e0edeb37d05789120975646},
	author = {Sadio, A. and Gustafsson, J.K. and Pereira, B. and Gomes, C.P. and Hansson, G.C. and David, L. and P{\^e}go, A.P. and Almeida, R.}
}
@article {3292,
	title = {Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications},
	journal = {Science and Technology of Advanced Materials},
	volume = {15},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol-gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior. {\textcopyright} 2014 National Institute for Materials Science.},
	keywords = {bacteria adhesion, Biomaterials, hemolysis, human dermal microvascular endothelial cells (HDMECs), human gingival fibroblasts (HGFs), Micropatterning, Soft lithography},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84898687729\&partnerID=40\&md5=f252b87aeb163775547158cad0cf658b},
	author = {Laranjeira, M.S. and Carvalho, {\^A}. and Pelaez-Vargas, A. and Hansford, D. and Ferraz, M.P. and Coimbra, S. and Costa, E. and Santos-Silva, A. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {3321,
	title = {Natriuretic peptide system modulation in uninephrectomized rats},
	journal = {Experimental and Clinical Cardiology},
	volume = {20},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2368 - 2387},
	abstract = {BACKGROUND: Cardiac activation of the natriuretic peptide (NP) system plays a major role in the renal adaptations to volume, expansion (VE). However, its role after uninephrectomy (Unx) remains to be elucidated. In the present study, we evaluated cardiac hemodynamics, plasma NP-Type B (BNP) levels nd NP receptor A (NPR-A) and C (NPR-C) expression in the renal cortex (RC) and medulla (RM) of Sham and Unx rats. METHODS: Male Wistar-Han rats (n=42) were randomly assigned to Unx or Sham surgery. Non-invasive blood pressure (BP), invasive left ventricular (LV) hemodynamics and natriuretic response to VE were performed and plasma was collected for BNP determination, 2, 10 and 26 weeks after surgery. In addition, samples from RC and RM were obtained for NPR-A and -C quantification (RT-PCR and western-blot). RESULTS: In Unx, blood pressure elevation and reduced natriuretic response to VE were observed. Although plasma BNP levels increased overtime, no LV dysfunction or myocardial BNP gene activation was observed in Unx. NPR-A mRNA in RC and RM were significantly increased in Unx at 26 weeks. This was accompanied by decreased NPR-C mRNA in the RM in Unx at 10 and 26 weeks, with no differences detected in the RC. CONCLUSIONS: In our experimentalmodel of Unx, progressive BP elevation and reduced natriuretic response to VE were accompanied by modulation of NPR-A and -C expression, in both the RC and RM, in the absence of cardiac activation of the NP system. Our results suggest a local renal modulation of the NP system after Unx. {\textcopyright} 2013 et al.; licensee Cardiology Academic Press.},
	keywords = {BNP, Hypertension, Natriuretic peptide receptor, Uninephrectomy},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905968553\&partnerID=40\&md5=54369af569474ee0bfddfa5aeac3237a},
	author = {Santos-Ara{\'u}jo, C. and Roncon-Albuquerque Jr., R. and Moreira-Rodrigues, M. and Henriques-Coelho, T. and Quelhas-Santos, J. and Faria, B. and Sampaio-Maia, B. and Leite-Moreira, A.F. and Pestana, M.}
}
@article {3269,
	title = {Optimization of the production of solid Witepsol nanoparticles loaded with rosmarinic acid},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {115},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {109 - 117},
	abstract = {During the last decade there has been a growing interest in the formulation of new food and nutraceutical products containing compounds with antioxidant activity. Unfortunately, due to their structure, certain compounds such as polyphenols, in particular rosmarinic acid (RA) are not stable and may interact easily with matrices in which they are incorporated. To overcome such limitations, the formulation of loaded polyphenols nanoparticles can offer an efficient solution to protect such compounds. Based on this rationale, the aim of this study was to prepare solid lipid nanoparticles (SLNs) loaded with RA using a hot melt ultrasonication method, where Witepsol H15 was used as lipid and Polysorbate 80 (Tween 80) as surfactant, following a 32 fractional factorial design, resulting in the use of 3 different percentages of surfactant (viz. 1, 2 and 3\%, v/v) and lipid (0.5, 1.0 and 1.5\%, w/v). The stability of the nanoparticles systems were tested during 28d in aqueous solution stored at refrigeration temperature (ca. 5{\textdegree}C), tracking the mean particle size of different formulations by photon correlation spectroscopy. To confirm RA entrapment, thermal analyses of the nanoparticles by DSC and FTIR were performed. The association efficiencies percentages (AE\%) were determined using HPLC to quantitatively assess the RA in supernatants. Results showed that Witepsol H15 produced nanoparticles with initial mean diameters between 270 and 1000nm, yet over time, a slight increase occurred, but without occurrence of aggregation. The AE\% showed a high percentage of encapsulation (ca. 99\%), which reveals low polyphenol releases from SLNs throughout storage time. In general, results showed a successful production of SLNs with properties that can be used to food applications. {\textcopyright} 2013 Elsevier B.V.},
	keywords = {Optimization, Rosmarinic acid, SLNs, Witepsol},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84892548596\&partnerID=40\&md5=231718083d6238d5ac676d642e12b0f8},
	author = {Campos, D.A. and Madureira, A.R. and Gomes, A.M. and Sarmento, B. and Pintado, M.M.}
}
@article {3313,
	title = {Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients},
	journal = {Experimental Biology and Medicine},
	volume = {239},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {502 - 508},
	abstract = {Renalase is a recently described enzyme secreted by the kidney into both plasma and urine, where it was suggested to degrade catecholamines contributing to blood pressure control. While there is a controversy regarding the relationship between renal function and plasma renalase levels, there is virtually no data in humans on plasma renalase activity as well as on both urine renalase levels and activity. We prospectively examined the time course of plasma and urine renalase levels and activity in 26 end-stage renal disease (ESRD) patients receiving a cadaver kidney transplant (cadaver kidney recipients [CKR]) before surgery and during the recovery of renal function up to day 90 post transplant. The relationship with sympathetic and renal dopaminergic activities was also evaluated. The recovery of renal function in CKR closely predicted decreases in plasma renalase levels (r = 0.88; P < 0.0001), urine renalase levels (r = 0.75; P < 0.0001) and urine renalase activity (r = 0.56; P < 0.03), but did not predict changes in plasma renalase activity (r = -0.02; NS). Plasma norepinephrine levels positively correlated with plasma renalase levels (r = 0.64, P < 0.002) as well as with urine renalase levels and activity (r = 0.47 P < 0.02; r = 0.71, P < 0.0005, respectively) and negatively correlated with plasma renalase activity (r = -0.57, P < 0.002). By contrast, plasma epinephrine levels positively correlated with plasma renalase activity (r = 0.67, P < 0.0001) and negatively correlated with plasma renalase levels (r = -0.62, P < 0.003). A significant negative relationship was observed between urine dopamine output and urine renalase levels (r = -0.48; P < 0.03) but not with urine renalase activity (r = -0.33, NS). We conclude that plasma and urine renalase levels closely depend on renal function and sympathetic nervous system activity. It is suggested that epinephrine-mediated activation of circulating renalase may occur in renal transplant recipients with good recovery of renal function. The increase in plasma renalase activity observed in ESRD patients and renal transplant recipients can be explained on the basis of reduced inhibition of the circulating enzyme. {\textcopyright} 2014 by the Society for Experimental Biology and Medicine.},
	keywords = {catecholamines, CKD, renal function, renal transplant, renalase},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84898964878\&partnerID=40\&md5=965164d696f11b12d4767bda11271186},
	author = {Quelhas-Santos, J. and Soares-Silva, I. and Fernandes-Cerqueira, C. and Sim{\~o}es-Silva, L. and Ferreira, I. and Carvalho, C. and Coentr{\~a}o, L. and Vaz, R. and Sampaio-Maia, B. and Pestana, M.}
}
@article {3416,
	title = {Plasma Renalase in Chronic Kidney Disease: Differences and Similarities between Humans and Rats},
	journal = {Current hypertension reviews},
	volume = {10},
	year = {2014},
	month = {2014},
	pages = {166-70},
	url = {<Go to ISI>://MEDLINE:25567503},
	author = {Quelhas-Santos, Janete and Pestana, Manuel}
}
@article {3316,
	title = {Poly(trimethylene carbonate-co-ε-caprolactone) promotes axonal growth},
	journal = {PLoS ONE},
	volume = {9},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Mammalian central nervous system (CNS) neurons do not regenerate after injury due to the inhibitory environment formed by the glial scar, largely constituted by myelin debris. The use of biomaterials to bridge the lesion area and the creation of an environment favoring axonal regeneration is an appealing approach, currently under investigation. This work aimed at assessing the suitability of three candidate polymers - poly(ε-caprolactone), poly(trimethylene carbonate-co-ε-caprolactone) (P(TMC-CL)) (11:89 mol\%) and poly(trimethylene carbonate) - with the final goal of using these materials in the development of conduits to promote spinal cord regeneration. Poly(L-lysine) (PLL) coated polymeric films were tested for neuronal cell adhesion and neurite outgrowth. At similar PLL film area coverage conditions, neuronal polarization and axonal elongation was significantly higher on P(TMC-CL) films. Furthermore, cortical neurons cultured on P(TMC-CL) were able to extend neurites even when seeded onto myelin. This effect was found to be mediated by the glycogen synthase kinase 3β (GSK3β) signaling pathway with impact on the collapsin response mediator protein 4 (CRMP4), suggesting that besides surface topography, nanomechanical properties were implicated in this process. The obtained results indicate P(TMC-CL) as a promising material for CNS regenerative applications as it promotes axonal growth, overcoming myelin inhibition. {\textcopyright} 2014 Rocha et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896121626\&partnerID=40\&md5=8f29642bf67ec86cf774379d7d92e7b0},
	author = {Rocha, D.N. and Brites, P. and Fonseca, C. and P{\^e}go, A.P.}
}
@article {3301,
	title = {Primary bone marrow mesenchymal stromal cells rescue the axonal phenotype of twitcher mice},
	journal = {Cell Transplantation},
	volume = {23},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {239 - 252},
	abstract = {Krabbe{\textquoteright}s disease (KD) is a demyelinating disorder caused by the deficiency of lysosomal galactocerebrosidase (GALC), affecting both the central (CNS) and the peripheral nervous system (PNS). A current therapy, hematopoietic stem cell transplantation (HSCT), is ineffective at correcting the PNS pathology. We have previously shown that systemic delivery of immortalized bone marrow-derived murine mesenchymal stromal cells (BM-MSCs) diminishes the neuropathology of transplanted Twitcher mice, a murine model of KD. In this study, to move one step closer to clinical application, the effectiveness of a systematic delivery of primary BM-MSCs to promote recovery of the Twitcher PNS was assessed. Primary BM-MSCs grafted to the Twitcher sciatic nerve led to increased GALC activity that was not correlated to decreased psychosine (the toxic GALC substrate) accumulation. Nevertheless, BM-MSC transplantation rescued the axonal phenotype of Twitcher mice in the sciatic nerve, with an increased density of both myelinated and unmyelinated axons in transplanted animals. Whereas no increase in myelination was observed, upon transplantation an increased proliferation of Schwann cell precursors occurred. Supporting these findings, in vitro, BM-MSCs promoted neurite outgrowth of Twitcher sensory neurons and proliferation of Twitcher Schwann cells. Moreover, BM-MSCs expressed nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) and promoted increased BDNF synthesis by neighboring Schwann cells. Besides their action in neurons and glia, BM-MSCs led to macrophage activation in Twitcher sciatic nerves. In summary, primary BM-MSCs diminish the neuropathology of Twitcher sciatic nerves by coordinately affecting neurons, glia, and macrophages. {\textcopyright} 2014 Cognizant Comm. Corp.},
	keywords = {Axonal degeneration, Bone marrow-derived mesenchymal stromal cells (BM-MSCs), Krabbe{\textquoteright}s disease (KD), Twitcher mice},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84893339069\&partnerID=40\&md5=51c17895a58b06f3b302616459dff62b},
	author = {Miranda, C.O. and Teixeira, C.A. and Sousa, V.F. and Santos,TE and Liz, M.A. and Marques,AM and Pinto-Do-{\'O}, P. and Sousa, M.M.}
}
@article {3277,
	title = {Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: The sea urchin Paracentrotus lividus},
	journal = {Marine Drugs},
	volume = {12},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {4912 - 4933},
	abstract = {Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is required, and marine organisms are among the safest and recently exploited ones. Sea urchins possess a circular area of soft tissue surrounding the mouth, the peristomial membrane (PM), mainly composed by mammalian-like collagen. The PM of the edible sea urchin Paracentrotus lividus therefore represents a potential unexploited collagen source, easily obtainable as a food industry waste product. Our results demonstrate that it is possible to extract native collagen fibrils from the PM and produce suitable substrates for in vitro system. The obtained matrices appear as a homogeneous fibrillar network (mean fibril diameter 30-400 nm and < 2 μm) and display remarkable mechanical properties in term of stiffness (146 {\textpm} 48 MPa) and viscosity (60.98 {\textpm} 52.07 GPa{\textperiodcentered}s). In vitro tests with horse pbMSC show a good biocompatibility in terms of overall cell growth. The obtained results indicate that the sea urchin P. lividus can be a valuable low-cost collagen source for mechanically resistant biomedical devices.},
	keywords = {Biocompatibility, Collagen matrix, Mesenchimal stromal cells, Native fibril, Sea urchin collagen, Tissue regeneration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84908025685\&partnerID=40\&md5=58774d56d0d60e6bc725ae6ecacaa068},
	author = {Di Benedetto, C. and Barbaglio, A. and Martinello, T. and Alongi, V. and Fassini, D. and Cullor{\`a}, E. and Patruno, M. and Bonasoro, F. and Barbosa, M.A. and Carnevali, M.D.C. and Sugni, M.}
}
@article {3267,
	title = {Rescue of wild-type E-cadherin expression from nonsense-mutated cancer cells by a suppressor-tRNA},
	journal = {European Journal of Human Genetics},
	volume = {22},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1085 - 1092},
	abstract = {Hereditary diffuse gastric cancer (HDGC) syndrome, although rare, is highly penetrant at an early age, and is severe and incurable because of ineffective screening tools and therapy. Approximately 45\% of HDGC families carry germline CDH1/E-cadherin alterations, 20\% of which are nonsense leading to premature protein truncation. Prophylactic gastrectomy is the only recommended approach for all asymptomatic CDH1 mutation carriers. Suppressor-tRNAs can replace premature stop codons (PTCs) with a cognate amino acid, inducing readthrough and generating full-length proteins. The use of suppressor-tRNAs in HDGC patients could therefore constitute a less invasive therapeutic option for nonsense mutation carriers, delaying the development of gastric cancer. Our analysis revealed that 23/108 (21.3\%) of E-cadherin-mutant families carried nonsense mutations that could be potentially corrected by eight suppressor-tRNAs, and arginine was the most frequently affected amino acid. Using site-directed mutagenesis, we developed an arginine suppressor-tRNA vector to correct one HDGC nonsense mutation. E-cadherin-deficient cell lines were transfected with plasmids carrying simultaneously the suppressor-tRNA and wild-type or mutant CDH1 mini-genes. RT-PCR, western blot, immunofluorescence, flow cytometry and proximity ligation assay (PLA) were used to establish the model, and monitor mRNA and protein expression and function recovery from CDH1 vectors. Cells expressing a CDH1 mini-gene, carrying a nonsense mutation and the suppressor-tRNA, recovered full-length E-cadherin expression and its correct localization and incorporation into the adhesion complex. This is the first demonstration of functional recovery of a mutated causative gene in hereditary cancer by cognate amino acid replacement with suppressor-tRNAs. Of the HDGC families, 21.3\% are candidates for correction with suppressor-tRNAs to potentially delay cancer onset. {\textcopyright} 2014 Macmillan Publishers Limited. All rights reserved.},
	keywords = {hereditary diffuse gastric cancer (HDGC), nonsense suppression, Premature termination codon (PTC), Suppressor-tRNA},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84906269658\&partnerID=40\&md5=2feed844660a13385857a7e5d4470a2b},
	author = {Bordeira-Carri{\c c}o, R. and Ferreira, D. and Mateus, D.D. and Pinheiro, H. and P{\^e}go, A.P. and Santos, M.A.S. and Oliveira, C.}
}
@article {3326,
	title = {Sca-1+cardiac progenitor cells and heart-making: A critical synopsis},
	journal = {Stem Cells and Development},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2263 - 2273},
	abstract = {The identification, in the adult, of cardiomyocyte turnover events and of cardiac progenitor cells (CPCs) has revolutionized the field of cardiovascular medicine. However, the low rate of CPCs differentiation events reported both in vitro and in vivo, even after injury, raised concerns on the biological significance of these subsets. In this Comprehensive Review, we discuss the current understanding of cardiac Lin-Sca-1+cells in light of what is also known for cellular compartments with similar phenotypes in other organs. The Lin-Sca-1+heart subset is heterogeneous and displays a mesenchymal profile, characterized by a limited ability to generate cardiomyocytes in vitro and in vivo, even after injury. There is no evidence for Sca-1 expression in embryonic cardiovascular progenitors. In other organs, Sca-1 expression is mainly observed on mesoderm-derived cells, although it is not restricted to stem/progenitor cell populations. It is urgent to determine, at a single cell level, to which extent cardiac Lin-Sca-1+cells overlap with the fibroblast compartment.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84907432293\&partnerID=40\&md5=f861431ef4750c567f4dff89b4bc013c},
	author = {Valente, M. and Nascimento, D.S. and Cumano, A. and Pinto-Do-{\'O}, P.}
}
@article {3285,
	title = {Selective albumin-binding surfaces modified with a thrombin-inhibiting peptide},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1227 - 1237},
	abstract = {Blood-contacting medical devices have been associated with severe clinical complications, such as thrombus formation, triggered by the activation of the coagulation cascade due to the adsorption of certain plasma proteins on the surface of biomaterials. Hence, the coating of such surfaces with antithrombotic agents has been used to increase biomaterial haemocompatibility. Biomaterial-induced clotting may also be decreased by albumin adsorption from blood plasma in a selective and reversible way, since this protein is not involved in the coagulation cascade. In this context, this paper reports that the immobilization of the thrombin inhibitor D-Phe-Pro-D-Arg-D-Thr-CONH 2 (fPrt) onto nanostructured surfaces induces selective and reversible adsorption of albumin, delaying the clotting time when compared to peptide-free surfaces. fPrt, synthesized with two glycine residues attached to the N-terminus (GGfPrt), was covalently immobilized onto self-assembled monolayers (SAMs) having different ratios of carboxylate-hexa(ethylene glycol)- and tri(ethylene glycol)-terminated thiols (EG6-COOH/EG3) that were specifically designed to control GGfPrt orientation, exposure and density at the molecular level. In solution, GGfPrt was able to inactivate the enzymatic activity of thrombin and to delay plasma clotting time in a concentration-dependent way. After surface immobilization, and independently of its concentration, GGfPrt lost its selectivity to thrombin and its capacity to inhibit thrombin enzymatic activity against the chromogenic substrate n-p-tosyl-Gly-Pro-Arg-p-nitroanilide. Nevertheless, surfaces with low concentrations of GGfPrt could delay the capacity of adsorbed thrombin to cleave fibrinogen. In contrast, GGfPrt immobilized in high concentrations was found to induce the procoagulant activity of the adsorbed thrombin. However, all surfaces containing GGfPrt have a plasma clotting time similar to the negative control (empty polystyrene wells), showing resistance to coagulation, which is explained by its capacity to adsorb albumin in a selective and reversible way. This work opens new perspectives to the improvement of the haemocompatibility of blood-contacting medical devices. {\textcopyright} 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Albumin, Blood compatibility, Clotting, Surface modification, Thrombin},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84895067841\&partnerID=40\&md5=8486186625f8f4f1cbc538bb3e34dd82},
	author = {Freitas, S.C. and Maia, S. and Figueiredo, A.C. and Gomes, P. and Pereira, P.J.B. and Barbosa, M.A. and Martins, M.C.L.}
}
@article {3398,
	title = {Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model},
	journal = {Journal of Clinical Investigation},
	volume = {124},
	year = {2014},
	note = {Bestas, Burcu Moreno, Pedro M. D. Blomberg, K. Emelie M. Mohammad, Dara K. Saleh, Amer F. Sutlu, Tolga Nordin, Joel Z. Guterstam, Peter Gustafsson, Manuela O. Kharazi, Shabnam Piatosa, Barbara Roberts, Thomas C. Behlke, Mark A. Wood, Matthew J. A. Gait, Michael J. Lundin, Karin E. El Andaloussi, Samir Mansson, Robert Berglof, Anna Wengel, Jesper Smith, C. I. Edvard},
	month = {2014},
	pages = {4067-4081},
	isbn = {0021-9738},
	url = {<Go to ISI>://WOS:000341168100038},
	author = {Bestas, B. and Moreno, P.M.D. and Blomberg, K. E. M. and Mohammad, D. K. and Saleh, A. F. and Sutlu, T. and Nordin, J. Z. and Guterstam, P. and Gustafsson, M. O. and Kharazi, S. and Piatosa, B. and Roberts, T. C. and Behlke, M. A. and Wood, M. J. A. and Gait, M. J. and Lundin, K.E. and El Andaloussi, S. and Mansson, R. and Berglof, A. and Wengel, J. and Smith, C.I.E.}
}
@article {3283,
	title = {Stable phenotype and function of immortalized Lin-Sca-1 + cardiac progenitor cells in long-term culture: A step closer to standardization},
	journal = {Stem Cells and Development},
	volume = {23},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1012 - 1026},
	abstract = {Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cardiac cell-based therapies. Although two distinct populations of CPCs reached the clinical setting, more detailed studies are required to portray the optimal cell type and therapeutic setting to drive robust cell engraftment and cardiomyogenesis after injury. Owing to the scarcity of the CPCs and the need for reproducibility, the generation of faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized Lin-Sca-1+ CPCs (iCPC Sca-1) represent their native-cell counterpart, thereby constituting a robust in vitro model system for standardized investigation in the cardiac field. iCPCSca-1 were established in vitro as plastic adherent cells endowed with robust self-renewal capacity while preserving a stable phenotype in long-term culture. iCPCSca-1 differentiated into cardiomyocytic-, endothelial-, and smooth muscle-like cells when subjected to appropriate stimuli. The cell line consistently displayed features of Lin -Sca-1+ CPCs in vitro, as well as in vivo after intramyocardial delivery in the onset of myocardial infarction (MI). Transplanted iCPCSca-1 significantly attenuated the functional and anatomical alterations caused by MI while promoting neovascularization. iCPCSca-1 are further shown to engraft, establish functional connections, and differentiate in loco into cardiomyocyte- and vasculature-like cells. These data validate iCPCSca-1 as an in vitro model system for Lin-Sca-1+ progenitors and for systematic dissection of mechanisms underlying CPC subsets engraftment/differentiation in vivo. Moreover, iCPCSca-1 can be regarded as a ready-to-use CPCs source for pre-clinical bioengineering studies toward the development of novel strategies for restoration of the damaged myocardium. {\textcopyright} Copyright 2014, Mary Ann Liebert, Inc. 2014.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896901797\&partnerID=40\&md5=35b7014573b8734d5c21f2089cdc038d},
	author = {Freire, AG and Nascimento, D.S. and Forte, G. and Valente, M. and Resende, TP and Pagliari, S. and Abreu, C and Carvalho, I and Nardo, P.D. and Pinto-Do-{\'O}, P.}
}
@article {3407,
	title = {Therapeutic antisense oligonucleotides against cancer: hurdling to the clinic},
	journal = {Frontiers in chemistry},
	volume = {2},
	year = {2014},
	note = {25353019},
	month = {2014},
	pages = {87},
	isbn = {2296-2646},
	url = {<Go to ISI>://MEDLINE:25353019},
	author = {Moreno, Pedro M. D. and Pego, Ana P.}
}
@article {3314,
	title = {Timing embryo segmentation: Dynamics and regulatory mechanisms of the vertebrate segmentation clock},
	journal = {BioMed Research International},
	volume = {2014},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {All vertebrate species present a segmented body, easily observed in the vertebrate column and its associated components, which provides a high degree of motility to the adult body and efficient protection of the internal organs. The sequential formation of the segmented precursors of the vertebral column during embryonic development, the somites, is governed by an oscillating genetic network, the somitogenesis molecular clock. Herein, we provide an overview of the molecular clock operating during somite formation and its underlying molecular regulatory mechanisms. Human congenital vertebral malformations have been associated with perturbations in these oscillatory mechanisms. Thus, a better comprehension of the molecular mechanisms regulating somite formation is required in order to fully understand the origin of human skeletal malformations. {\textcopyright} 2014 Tatiana P. Resende et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84901789504\&partnerID=40\&md5=48f7f79bd522d9e2cdd9568df8453874},
	author = {Resende, TP and Andrade, R.P. and Palmeirim, I.}
}
@article {3417,
	title = {Tools for address georeferencing - limitations and opportunities every public health professional should be aware of},
	journal = {PloS one},
	volume = {9},
	year = {2014},
	month = {2014},
	pages = {e114130-e114130},
	url = {<Go to ISI>://MEDLINE:25469514},
	author = {Ribeiro, Ana Isabel and Olhero, Andreia and Teixeira, Hugo and Magalhaes, Alexandre and Pina, Maria Fatima}
}
@article {3404,
	title = {Translating chitosan to clinical delivery of nucleic acid-based drugs},
	journal = {Mrs Bulletin},
	volume = {39},
	year = {2014},
	note = {Gomes, Carla Pereira Ferreira Lopes, Catia Daniela Duarte Moreno, Pedro Miguel Varela-Moreira, Aida Jose Alonso, Maria Pego, Ana PaulaPego, Ana Paula/C-6942-2012; Moreno, Pedro/J-9382-2013; Lopes, Catia/O-1090-2013
Pego, Ana Paula/0000-0001-5169-328X; Moreno, Pedro/0000-0002-3773-3262; Lopes, Catia/0000-0002-7467-6498
},
	month = {2014},
	pages = {60-70},
	isbn = {0883-7694},
	url = {<Go to ISI>://WOS:000330833200016},
	author = {Gomes, C.P. and Lopes, C.D.F. and Moreno, P.M.D. and Varela-Moreira, A. and Alonso, M.J. and Pego, A. P.}
}
@article {3309,
	title = {What do Portuguese women prefer regarding vaginal products? Results from a cross-sectional web-based survey},
	journal = {Pharmaceutics},
	volume = {6},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {543 - 556},
	abstract = {Therapeutic outcomes of vaginal products depend not only on their ability to deliver drugs to or through the vagina but also on acceptability and correct use. Women{\textquoteright}s preferences, in turn, may vary according to age and cultural backgrounds. In this work, an anonymous online survey was completed by 2529 Portuguese women to assess their preferences for physical characteristics and mode of application of vaginal products, according to age. Additionally, intention to use and misconceptions about these issues were assessed. The majority of women of all age groups would use vaginal products to treat or prevent diseases, upon medical prescription. Women preferred vaginal products to be odorless and colorless gels, creams and ointments composed by natural origin drugs/excipients and applied by means of an applicator. Although the majority of women would prefer not to insert any product in the vagina, intention to use for self and recommendation to use for others was associated with previous experiences with vaginal products. General concerns and misconceptions related to use of vaginal products were rare. These data may contribute to the development of products that women are more prone to use.},
	keywords = {Acceptability, Vaginal dosage forms, Vaginal products, Women{\textquoteright}s preferences},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84908210757\&partnerID=40\&md5=5515e40e97ff157b0a5b4498557008ef},
	author = {Palmeira-de-Oliveira, R. and Duarte, P. and Palmeira-de-Oliveira, A. and Das Neves, J. and Amaral, M.H. and Breitenfeld, L. and Martinez-de-Oliveira, J.}
}
@article {3414,
	title = {What do portuguese women prefer regarding vaginal products? Results from a cross-sectional web-based survey},
	journal = {Pharmaceutics},
	volume = {6},
	year = {2014},
	note = {25337676},
	month = {2014},
	pages = {543-56},
	isbn = {1999-4923},
	url = {<Go to ISI>://MEDLINE:25337676},
	author = {Palmeira-de-Oliveira, Rita and Duarte, Paulo and Palmeira-de-Oliveira, Ana and das Neves, Jose and Amaral, Maria Helena and Breitenfeld, Luiza and Martinez-de-Oliveira, Jose}
}
@article {2849,
	title = {Adult stem cells and biocompatible scaffolds as smart drug delivery tools for cardiac tissue repair},
	journal = {Current Medicinal Chemistry},
	volume = {20},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {3429 - 3447},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84883267656\&partnerID=40\&md5=b03789229cfac322e56bb69b4fc644ea},
	author = {Pagliari, S. and Romanazzo, S. and Mosqueira, D. and Pinto-Do-{\'O}, P. and Aoyagi, T. and Forte, G.}
}
@article {2871,
	title = {Advanced biofabrication strategies for skin regeneration and repair},
	journal = {Nanomedicine},
	volume = {8},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {603 - 621},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84875943184\&partnerID=40\&md5=6ad3f1ffd0f6a7e567b16f0fd9b31121},
	author = {Pereira, R.F. and Barrias, C.C. and Granja, P.L. and Bartolo, P.J.}
}
@article {2872,
	title = {Biocompatibility of poly(lactic acid) with incorporated graphene-based materials},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {104},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {229 - 238},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84872444845\&partnerID=40\&md5=7d61d767da7f0776cca70616b06301a9},
	author = {Pinto, A.M. and Moreira, S. and Gon{\c c}alves, I.C. and Gama, F.M. and Mendes, A.M. and Magalh{\~a}es, F.D.}
}
@article {2835,
	title = {Bioengineered surfaces promote specific protein-glycan mediated binding of the gastric pathogen Helicobacter pylori},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {8885 - 8893},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84885052038\&partnerID=40\&md5=0d17eb0f95d76eb18ea20ef7f33c8192},
	author = {Parreira, P. and Magalh{\~a}es, A. and Reis, C.A. and Bor{\'e}n, T. and Leckband, D. and Martins, M.C.L.}
}
@article {2888,
	title = {Characterization of human NLZ1/ZNF703 identifies conserved domains essential for proper subcellular localization and transcriptional repression},
	journal = {Journal of Cellular Biochemistry},
	volume = {114},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {120 - 133},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84869412286\&partnerID=40\&md5=e48a26e94d7ce0eddb65d8067fdc056c},
	author = {Pereira-Castro, I. and Costa, {\^A}.M.S. and Oliveira, M.J. and Barbosa, I. and Rocha, A.S. and Azevedo, L. and Da Costa, L.T.}
}
@article {2837,
	title = {Development and validation method for simultaneous quantification of phenolic compounds in natural extracts and nanosystems},
	journal = {Phytochemical Analysis},
	volume = {24},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {638 - 644},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84886723210\&partnerID=40\&md5=eb1de8e3010b22c26b7ddd2e09eef6a7},
	author = {Da Silva, S.B. and Oliveira, A. and Ferreira, D. and Sarmento, B. and Pintado, M.}
}
@article {2878,
	title = {Development of bis-locked nucleic acid (bisLNA) oligonucleotides for efficient invasion of supercoiled duplex DNA},
	journal = {Nucleic Acids Research},
	volume = {41},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {3257 - 3273},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876401355\&partnerID=40\&md5=96078ee73fe175f8f962db91c20a8724},
	author = {Moreno, P.M.D. and Geny, S. and Pabon, Y.V. and Bergquist, H. and Zaghloul, E.M. and Rocha, C.S.J. and Oprea, I.I. and Bestas, B. and Andaloussi, S.E. and J{\o}rgensen, P.T. and Pedersen, E.B. and Lundin, K.E. and Zain, R. and Wengel, J. and Smith, C.I.E.}
}
@article {2834,
	title = {Development of PLGA-mannosamine nanoparticles as oral protein carriers},
	journal = {Biomacromolecules},
	volume = {14},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {4046 - 4052},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84887602933\&partnerID=40\&md5=ee1468ca288d3ca59120be3637a51b41},
	author = {Alonso-Sande, M. and Des Rieux, A. and Fievez, V. and Sarmento, B. and Delgado, A. and Evora, C. and Remu{\~n}{\'a}n-L{\'o}pez, C. and Pr{\'e}at, V. and Alonso, M.J.}
}
@article {2879,
	title = {E-cadherin and adherens-junctions stability in gastric carcinoma: Functional implications of glycosyltransferases involving N-glycan branching biosynthesis, N-acetylglucosaminyltransferases III and v},
	journal = {Biochimica et Biophysica Acta - General Subjects},
	volume = {1830},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {2690 - 2700},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873589547\&partnerID=40\&md5=ea238ee2045a2bbfb910b24526aac863},
	author = {Pinho, S.S. and Figueiredo, J. and Cabral, J. and Carvalho, S. and Dourado, J. and Magalh{\~a}es, A. and G{\"a}rtner, F. and Mendon{\c c}a, A.M. and Isaji, T. and Gu, J. and Carneiro, F. and Seruca, R. and Taniguchi, N. and Reis, C.A.}
}
@article {2856,
	title = {Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration - In vitro study},
	journal = {Journal of Biomedical Materials Research - Part B Applied Biomaterials},
	volume = {101 B},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {762 - 769},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84878874447\&partnerID=40\&md5=edd0491ee0c1e8faf857a483e14efaf9},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Carvalho, A. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
@article {2880,
	title = {Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7-10)},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {5643 - 5652},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873199566\&partnerID=40\&md5=f548695261ada2fc57ece26bff1f45d0},
	author = {Amaral, I.F. and Neiva, I. and Ferreira da Silva, F. and Sousa, S.R. and Piloto, A.M. and Lopes, C.D.F. and Barbosa, M.A. and Kirkpatrick, C.J. and P{\^e}go, A.P.}
}
@book {2848,
	title = {Engineering of a 3D nanostructured scaffold made of functionalized self-assembling peptides and encapsulated neural stem cells},
	series = {Methods in Molecular Biology},
	volume = {1058},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {171 - 182},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84883213165\&partnerID=40\&md5=678409375bfa31b81139a8e44e0f205f},
	author = {Cunha, C. and Panseri, S. and Gelain, F.}
}
@article {2859,
	title = {Expression of ST3GAL4 Leads to SLex Expression and Induces c-Met Activation and an Invasive Phenotype in Gastric Carcinoma Cells},
	journal = {PLoS ONE},
	volume = {8},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: ScopusArt. No.: e66737},
	month = {2013///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879165131\&partnerID=40\&md5=58617c0600b708de7a68556b87336112},
	author = {Gomes, C. and Os{\'o}rio, H. and Pinto, M.T. and Campos, D. and Oliveira, M.J. and Reis, C.A.}
}
@article {2839,
	title = {Graphene-based materials biocompatibility: A review},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {111},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {188 - 202},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879808520\&partnerID=40\&md5=ae9e0dbaf79ff79a5f60800ac798b26d},
	author = {Pinto, A.M. and Gon{\c c}alves, I.C. and Magalh{\~a}es, F.D.}
}
@article {2862,
	title = {High biocompatibility and improved osteogenic potential of novel Ca-P/titania composite scaffolds designed for regeneration of load-bearing segmental bone defects},
	journal = {Journal of Biomedical Materials Research - Part A},
	volume = {101 A},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1612 - 1619},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876296528\&partnerID=40\&md5=6721f219742ba18cfd907ca516ddcd07},
	author = {Cunha, C. and Sprio, S. and Panseri, S. and Dapporto, M. and Marcacci, M. and Tampieri, A.}
}
@article {2882,
	title = {Highly focalised thermotherapy using a ferrimagnetic cement in the treatment of a melanoma mouse model by low temperature hyperthermia},
	journal = {International Journal of Hyperthermia},
	volume = {29},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {121 - 132},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84874056021\&partnerID=40\&md5=638aa112bc0f2f9939db5b58b5a2525b},
	author = {Portela, A. and Vasconcelos, M. and Fernandes, M.H. and Garcia, M. and Silva, A. and Gabriel, J. and Gartner, F. and Amorim, I. and Cavalheiro, J.}
}
@article {2866,
	title = {Hyaluronic acid / chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes},
	journal = {Journal of Controlled Release},
	volume = {168},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {289 - 297},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877033262\&partnerID=40\&md5=d58ff56593b3a791351102271ca61440},
	author = {Hartmann, H. and Hossfeld, S. and Schlosshauer, B. and Mittnacht, U. and P{\^e}go, A.P. and Dauner, M. and Doser, M. and Stoll, D. and Krastev, R.}
}
@article {2892,
	title = {The importance of antimicrobial peptides and their potential for therapeutic use in ophthalmology},
	journal = {International Journal of Antimicrobial Agents},
	volume = {41},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {5 - 10},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84871656457\&partnerID=40\&md5=ca0df69f419a89c6d0ba7d1b73fcb9d0},
	author = {Silva, N.C. and Sarmento, B. and Pintado, M.}
}
@article {2889,
	title = {Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness},
	journal = {Human Molecular Genetics},
	volume = {22},
	year = {2013},
	note = {Cited By (since 1996):2Export Date: 12 December 2013Source: ScopusArt. No.: dds405},
	month = {2013///},
	pages = {84 - 95},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84871200220\&partnerID=40\&md5=b2bfd70fb2a8a4e517e4ad698a2461cc},
	author = {Moniz, S. and Martinho, O. and Pinto, F. and Sousa, B. and Loureiro, C. and Oliveira, M.J. and Moita, L.F. and Honavar, M. and Pinheiro, C. and Pires, M. and Lopes, J.M. and Jones, C. and Costello, J.F. and Paredes, J. and Reis, R.M. and Jordan, P.}
}
@booklet {2867,
	title = {Macrophages stimulate gastric and colorectal cancer invasion through EGFR Y1086, c-Src, Erk1/2 and Akt phosphorylation and smallGTPase activity},
	howpublished = {Oncogene},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: ScopusArticle in Press},
	month = {2013///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876908646\&partnerID=40\&md5=bcc2929bb93817db3dc0e3421c420722},
	author = {Cardoso, A.P. and Pinto, M.L. and Pinto, A.T. and Oliveira, M.I. and Pinto, M.T. and Gon{\c c}alves, R. and Relvas, J.B. and Figueiredo, C. and Seruca, R. and Mantovani, A. and Mareel, M. and Barbosa, M.A. and Oliveira, M.J.}
}
@article {2853,
	title = {Medicago spp. extracts as promising ingredients for skin care products},
	journal = {Industrial Crops and Products},
	volume = {49},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {634 - 644},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84880378375\&partnerID=40\&md5=1b60aaf1efb444f653b44800529f5ae3},
	author = {Rodrigues, F. and Palmeira-de-Oliveira, A. and Das Neves, J. and Sarmento, B. and Amaral, M.H. and Oliveira, M.B.}
}
@article {2843,
	title = {Neuropeptide y modulates fracture healing through Y1 receptor signaling},
	journal = {Journal of Orthopaedic Research},
	volume = {31},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1570 - 1578},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84882886747\&partnerID=40\&md5=dd7576a200a42ce37ff5a98115fe9462},
	author = {Sousa, D.M. and McDonald, M.M. and Mikulec, K. and Peacock, L. and Herzog, H. and Lamghari, M. and Little, D.G. and Baldock, P.A.}
}
@article {2874,
	title = {Osteoporotic hip fractures: Bisphosphonates sales and observed turning point in trend. A population-based retrospective study},
	journal = {Bone},
	volume = {53},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {430 - 436},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873288549\&partnerID=40\&md5=da493b87546091513eb8b54c3748cda6},
	author = {Alves, S.M. and Economou, T. and Oliveira, C. and Ribeiro, A.I. and Neves, N. and Gom{\'e}z-Barrena, E. and Pina, M.F.}
}
@article {2865,
	title = {Overexpression of tumour-associated carbohydrate antigen sialyl-Tn in advanced bladder tumours},
	journal = {Molecular Oncology},
	volume = {7},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {719 - 731},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84878107131\&partnerID=40\&md5=52c1f1e8bc83224d947840df0f3dd539},
	author = {Ferreira, J.A. and Videira, P.A. and Lima, L. and Pereira, S. and Silva, M. and Carrascal, M. and Severino, P.F. and Fernandes, E. and Almeida, A. and Costa, C. and Vitorino, R. and Amaro, T. and Oliveira, M.J. and Reis, C.A. and Dall{\textquoteright}Olio, F. and Amado, F. and Santos, L.L.}
}
@article {2831,
	title = {Sociodemographic determinants of prevalence and incidence of helicobacter pylori infection in portuguese adults},
	journal = {Helicobacter},
	volume = {18},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {413 - 422},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84886298522\&partnerID=40\&md5=460550c9c4474e105c93815a934c98ee},
	author = {Bastos, J. and Peleteiro, B. and Barros, R. and Alves, L. and Severo, M. and de F{\'a}tima Pina, M. and Pinto, H. and Carvalho, S. and Marinho, A. and Guimar{\~a}es, J.T. and Azevedo, A. and La Vecchia, C. and Barros, H. and Lunet, N.}
}
@article {2900,
	title = {Stable phenotype and function of immortalized Lin-Sca-1+ cardiac progenitor cells in long-term culture: a step closer to standardization.},
	journal = {Stem Cells Dev},
	volume = {Epub ahead of print},
	year = {2013},
	month = {12-2013},
	isbn = {PMID: 24367889 },
	url = {http://www.ncbi.nlm.nih.gov/pubmed/24367889},
	author = {Freire, AG and Nascimento, D.S. and Forte, G. and Valente, M. and Resende, TP and Pagliari, S. and Abreu, C and Carvalho, I and Di Nardo, P. and Pinto-Do-{\'O}, P.}
}
@article {2881,
	title = {Taking tissue adhesives to the future: From traditional synthetic to new biomimetic approaches},
	journal = {Biomaterials Science},
	volume = {1},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {239 - 253},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877753912\&partnerID=40\&md5=772c15e73287e3754da848ada5a0e296},
	author = {Br{\'e}, L.P. and Zheng, Y. and P{\^e}go, A.P. and Wang, W.}
}
@article {2796,
	title = {Taking tissue adhesives to the future: from traditional synthetic to new biomimetic approaches},
	volume = {1},
	year = {2013},
	month = {2013},
	pages = {239 - 253},
	publisher = {Royal Society of Chemistry},
	author = {Br{\'e}, L{\'\i}gia Pereira and Zheng, Yu and P{\^e}go, Ana Paula and Wang, Wenxin}
}
@conference {2192,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical ImagingIEEE Comput. Soc. Conf. Comput. Vis. Pattern Recogn.},
	series = {2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012},
	year = {2012},
	note = {Conference code: 91746Export Date: 21 November 2012Source: ScopusArt. No.: 6235546doi: 10.1109/ISBI.2012.6235546Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Lutgens, E., Daemen, M., Muinck, E., Chronic myocardial infarction in the mouse: Cardiac structural and functional changes (1998) Cardiovascular Research, 41, pp. 586-593; 
Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Nascimento, D., Valente, M., Esteves, T., Pina, M.F., Guedes, J.G., Freire, A., Quelhas, P., Pinto-Do, O.P., Miquant - Semi-automation of infarct size assessment in models of cardiac ischemic injury (2011) Plos One, 6 (9), pp. 1-8; 
Esteves, T., Valente, M., Nascimento, D., Pinto-Do, O.P., Quelhas, P., Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model (2011) Proceedings of IbPRIA, LNCS, 6669, pp. 151-158; 
Pfeffer, M., Pfeffer, J., Fishbein, M., Myocardial infarct size and ventricular function in rats (1979) Circulation Research, 44, pp. 503-512
Sponsors: The Institute of Electrical and Electronic Engineers; Engineering in Medicine and Biology Society; Signal Processing Society},
	month = {2012///},
	pages = {310 - 313},
	address = {Barcelona},
	abstract = {Experimental rodent models of induced ischemic injury have been extensively used in biomedical research to study molecular, cellular and histological alterations following myocar-dial infarction. These models are increasingly employed to assess the potential of newly developed therapies for functional restoration of the damaged heart. Such studies are based on myocardial infarction induction followed by different therapeutic interventions and subsequent analysis of the infarct size. This analysis is used to evaluate the extent to which such interventions meet recovery of the lost myocardial tissue. Infarct size is defined as the percentage of the left ventricle affected by coronary artery occlusion. The infarct size is traditionally estimated manually delineating the infarcted and normal tissue areas in the left ventricle of the excised heart. However, this is a time-consuming, arduous and prone to bias process. Herein, we developed an anatomic model, adapted through expectation maximization, which allows for fully automatic analysis of the data. Experimental validation is performed comparing the proposed approach with manual annotation. The results obtained through anatomical model adaptation were coherent with those manually obtained and the differences where never higher than 10\%. {\textcopyright} 2012 IEEE.},
	keywords = {Anatomic models, anatomical model, Anatomical models, Automatic analysis, Biomedical research, Coronary arteries, expectation maximization, Experimental validations, Image segmentation, Infarct size, Ischemic injuries, Left ventricles, Manual annotation, Maximum principle, Medical imaging, Murine model, Myocardial Infarction, Myocardial infarction size, Myocardial tissue, Normal tissue, Rodent models, Therapeutic intervention, Tissue},
	isbn = {19457928 (ISSN); 9781457718588 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=087bcb09e5d5319029623283cba34c34},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@conference {2817,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical Imaging},
	year = {2012},
	note = {Export Date: 2 October 2013Source: ScopusArt. No.: 6235546},
	month = {2012///},
	pages = {310 - 313},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=6716e8eb5d812d3e0ffe54d87369d746},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@booklet {2190,
	title = {Bioengineered surfaces to improve the blood compatibility of biomaterials through direct thrombin inactivation},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.07.020Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre 823, 415email: cmartins@ineb.up.pt},
	month = {2012///},
	abstract = {Thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. This work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. Boophilin, a direct thrombin inhibitor from the cattle tick Rhipicephalus microplus, was produced as a recombinant protein in Pichia pastoris. Boophilin was biotinylated and immobilized on biotin-terminated self-assembled monolayers (SAM) via neutravidin. In order to maintain its proteinase inhibitory capacity after surface immobilization, boophilin was biotinylated after the formation of a boophilin-thrombin complex to minimize the biotinylation of the residues involved in thrombin-boophilin interaction. The extent of boophilin biotinylation was determined using matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. Boophilin immobilization and thrombin adsorption were quantified using quartz crystal microbalance with dissipation. Thrombin competitive adsorption from human serum was assessed using                        125I-thrombin. Thrombin inhibition and plasma clotting time were determined using spectrophotometric techniques. Boophilin-coated SAM were able to promote thrombin adsorption in a selective way, inhibiting most of its activity and delaying plasma coagulation in comparison with boophilin-free surfaces, demonstrating boophilin{\textquoteright}s potential to improve the hemocompatibility of biomaterials used in the production of blood-contacting devices. {\textcopyright} 2012 Acta Materialia Inc.
},
	keywords = {Coagulation, Hemocompatibility, Protein adsorption, Surface functionalization, Thrombin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865246462\&partnerID=40\&md5=278b2e7d1427a6032eba9ecbd01c3872},
	author = {Freitas, S.C. and Cereija, T.B. and Figueiredo, A.C. and Os{\'o}rio, H. and Pereira, P.J.B. and Barbosa, M.A. and Martins, M.C.L.}
}
@article {2203,
	title = {Biosynthesis of highly pure poly-γ-glutamic acid for biomedical applications},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {23},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-012-4639-xPubMed ID: 22532096Language of Original Document: EnglishCorrespondence Address: Pereira, C.L.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: Catarina.pereira@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; polyglutamic acid, 24991-23-9, 25513-46-6; Biocompatible Materials; Polyglutamic Acid, 25513-46-6; poly(gamma-glutamic acid)References: Ratner, B.D., Hoffman, A.S., Shoen, F.J., Lemons, J.E., (2004) Natural Materials. Biomaterials Science-an Introduction to Materials in Medicine, , 2nd ed. San Diego, CA: Academic Press;Buescher, J.M., Margaritis, A., Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries (2007) Critical Reviews in Biotechnology, 27 (1), pp. 1-19. , DOI 10.1080/07388550601166458, PII 773236840; 
Kang, H.-S., Park, S.-H., Lee, Y.-G., Son, T.-I., Polyelectrolyte complex hydrogel composed of chitosan and poly(γ-glutamic acid) for biological application: Preparation, physical properties, and cytocompatibility (2007) Journal of Applied Polymer Science, 103 (1), pp. 386-394. , DOI 10.1002/app.24623; 
Lin, Y.-H., Chung, C.-K., Chen, C.-T., Liang, H.-F., Chen, S.-C., Sung, H.-W., Preparation of nanoparticles composed of chitosan/poly-γ-glutamic acid and evaluation of their permeability through Caco-2 cells (2005) Biomacromolecules, 6 (2), pp. 1104-1112. , DOI 10.1021/bm049312a; 
Sanda, F., Fujiyama, T., Endo, T., Chemical synthesis of polygamma-glutamic acid by polycondensation of gamma-glutamic acid dimer: Synthesis and reaction of poly gamma-glutamic acid methyl ester (2000) J Polym Sci, Part A: Polym Chem, 39, pp. 732-741; 
Ashiuchi, M., Shimanouchi, K., Nakamura, H., Kamei, T., Soda, K., Park, C., Sung, M.-H., Misono, H., Enzymatic synthesis of high-molecular-mass poly-γ-glutamate and regulation of its stereochemistry (2004) Applied and Environmental Microbiology, 70 (7), pp. 4249-4255. , DOI 10.1128/AEM.70.7.4249-4255.2004; 
Shih, I.-L., Van, Y.-T., The production of poly-(γ-glutamic acid) from microorganisms and its various applications (2001) Bioresource Technology, 79 (3), pp. 207-225. , DOI 10.1016/S0960-8524(01)00074-8, PII S0960852401000748; 
Prodhomme, E.J.F., Tutt, A.L., Glennie, M.J., Bugg, T.D.H., Multivalent Conjugates of Poly-γ-D-glutamic Acid from Bacillus licheniformis with Antibody F(ab{\textquoteright}) and Glycopeptide Ligands (2003) Bioconjugate Chemistry, 14 (6), pp. 1148-1155. , DOI 10.1021/bc020019m; 
Schneerson, R., Kubler-Kielb, J., Liu, T.-Y., Dai, Z.-D., Leppla, S.H., Yergey, A., Backlund, P., Robbins, J.B., Poly(γ-D-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: A potential addition to the anthrax vaccine (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8945-8950. , DOI 10.1073/pnas.1633512100; 
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Sung, M.-H., Park, C., Kim, C.-J., Poo, H., Soda, K., Ashiuchi, M., Natural and edible biopolymer poly-γ-glutamic acid: Synthesis, production, and applications (2005) Chemical Record, 5 (6), pp. 352-366. , DOI 10.1002/tcr.20061; 
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Park, C., Choi, J.-C., Choi, Y.-H., Nakamura, H., Shimanouchi, K., Horiuchi, T., Misono, H., Sung, M.-H., Synthesis of super-high-molecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. chungkookjang (2005) Journal of Molecular Catalysis B: Enzymatic, 35 (4-6), pp. 128-133. , DOI 10.1016/j.molcatb.2005.06.007; 
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Kubota, H., Matsunobu, T., Uotani, K., Takebe, H., Satoh, A., Tanaka, T., Production of poly(c-glutamic acid) by Bacillus subtilis F-2-01 (1993) Biosci Biotechnol Biochem, 57 (7), pp. 1212-1213; 
Kunioka, M., Goto, A., Biosynthesis of poly(γ-glutamic acid) from L-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335 (1994) Applied Microbiology and Biotechnology, 40 (6), pp. 867-872. , DOI 10.1007/s002530050082; 
Ashiuchi, M., Misono, H., Biochemistry and molecular genetics of poly-γ-glutamate synthesis (2002) Applied Microbiology and Biotechnology, 59 (1), pp. 9-14. , DOI 10.1007/s00253-002-0984-x; 
Hsieh, C.-Y., Tsai, S.-P., Wang, D.-M., Chang, Y.-N., Hsieh, H.-J., Preparation of γ-PGA/chitosan composite tissue engineering matrices (2005) Biomaterials, 26 (28), pp. 5617-5623. , DOI 10.1016/j.biomaterials.2005.02.012, PII S0142961205001614; 
Richard, A., Margaritis, A., Rheology, oxygen transfer, and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis (2003) Biotechnology and Bioengineering, 82 (3), pp. 299-305. , DOI 10.1002/bit.10568; 
Peng, S.-F., Yang, M.-J., Su, C.-J., Chen, H.-J., Lee, P.-W., Wei, M.-C., Effects of incorporation of poly(gamma-glutamic acid) in chitosan/DNA complex nanoparticles on cellular uptake and transfection efficiency (2009) Biomaterials, 30, pp. 1797-1808; 
Lin, Y.-H., Mi, F.-L., Chen, C.-T., Chang, W.-C., Peng, S.-F., Liang, H.-F., Sung, H.-W., Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery (2007) Biomacromolecules, 8 (1), pp. 146-152. , DOI 10.1021/bm0607776; 
Lee, P.-W., Peng, S.-F., Su, C.-J., Mi, F.-L., Chen, H.-L., Wei, M.-C., Lin, H.-J., Sung, H.-W., The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery (2008) Biomaterials, 29 (6), pp. 742-751. , DOI 10.1016/j.biomaterials.2007.10.034, PII S014296120700837X; 
Hajdu, I., Bodn{\'a}r, M., Filipcsei, G., Hartmann, J., Dar{\'o}czi, L., Zr{\'\i}nyi, M., Nanoparticles prepared by self-assembly of Chitosan and poly-γ-glutamic acid (2008) Colloid Polym Sci, 286 (3), pp. 343-350; 
Keresztessy, Z., Bodn{\'a}r, M., Ber, E., Hajdu, I., Zhang, M., Hartmann, J., Self-assembling chitosan/poly-γ-glutamic acid nanoparticles for targeted drug delivery (2009) Colloid Polym Sci, 287 (7), pp. 759-765; 
Nelson, D., Cox, M., Amino acids peptides and proteins (2005) Lehnninger Principles of Biochemistry, pp. 78-79. , Freeman WH editor. New York: WH Freemand and Company; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57 (1), pp. 35-52. , DOI 10.1016/S0939-6411(03)00160-7; 
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Antunes, J.C., Pereira, C.L., Molinos, M., Ferreira-Da-Silva, F., Gon{\c c}alves, R.M., Barbosa, M.A., Layer-by-layer self-assembly of polyelectrolyte complexes of Chitosam and poly(gamma-glutamic acid) (2011) Biomacromolecules, 12 (12), pp. 4183-4195; 
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Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L., Alonso, M.J., Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers (1997) Journal of Applied Polymer Science, 63 (1), pp. 125-132
},
	month = {2012///},
	pages = {1583 - 1591},
	abstract = {The remarkable properties of poly-aminoacids, mainly their biocompatibility and biodegradability, have prompted an increasing interest in these polymers for biomedical applications. Poly-γ-glutamic acid (γ-PGA) is one of the most interesting poly-aminoacids with potential applications as a biomaterial. Here we describe the production and characterization of γ-PGA by Bacillus subtilis natto. The γ-PGA was produced with low molecular weight (10-50 kDa), high purity grade (>99 \%) and a D-/L-glutamate ratio of 50-60/50-40 \%. To evaluate the feasibility of using this γ-PGA as a biomaterial, chitosan (Ch)/γ-PGA nanoparticles were prepared by the coacervation method at pH ranging from 3.0 to 5.0, with dimensions in the interval 214-221 nm with a poly-dispersion index of ca. 0.2. The high purity of γ-PGA produced by this method, which is firstly described here, renders this biopolymer suitable for biomedical applications. Moreover, the Ch/γ-PGA nanocomplexes developed in this investigation can be combined with biologically active substances for their delivery in the organism. The fact that the assembly between Ch and γ-PGA relies on electrostatic interactions enables addition of other molecules that can be released into the medium through changes from acidic to physiological pH, without loss in biological activity. {\textcopyright} Springer Science+Business Media, LLC 2012.},
	keywords = {Amino acids, animal cell, article, Bacillus subtilis, Bacillus subtilis natto, Bioactivity, Biocompatibility, Biocompatible Materials, biodegradability, Biodegradable polymers, Biodegradation, Biologically active substances, biomaterial, Biomedical applications, biomedicine, biosynthesis, chitosan, circular dichroism, complex formation, controlled study, dispersion, Electrophoresis, Polyacrylamide Gel, feasibility study, Functional polymers, gamma polyglutamic acid, Glutamic acid, High purity, Low molecular weight, Medical applications, molecular interaction, molecular weight, Nanocomplexes, nanoparticle, nonhuman, pH, Physiological pH, polyglutamic acid, Potential applications, priority journal, purification, Spectroscopy, Fourier Transform Infrared, static electricity, Stereoisomerism, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84863314791\&partnerID=40\&md5=d0d618bb2684c8e80e984cfe35f3eb77},
	author = {Pereira, C.L. and Antunes, J.C. and Gon{\c c}alves, R.M. and Ferreira-da-Silva, F. and Barbosa, M.A.}
}
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	year = {2012},
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},
	month = {2012///},
	pages = {667 - 678},
	abstract = {Several hereditary cancer syndromes are associated with nonsense mutations that create premature termination codons (PTC). Therapeutic strategies involving readthrough induction partially restore expression of proteins with normal function from nonsense-mutated genes, and small molecules such as aminoglycosides and PTC124 have exhibited promising results for treating patients with cystic fibrosis and Duchenne muscular dystrophy. Transgenic expression of suppressor-tRNAs and depleting translation termination factors are, among others, potential strategies for treating PTC-associated diseases. In this review, the potential of using readthrough strategies as a therapy for cancer syndromes is discussed, and we consider the effect of nonsense-mediated decay and other factors on readthrough efficiency. {\textcopyright} 2012 Elsevier Ltd.},
	keywords = {Aminoglycosides, Hereditary cancer syndromes, Nonsense-mediated decay (NMD), Nonsense-suppression, Premature termination codon (PTC), PTC124, Readthrough, Suppressor-tRNA},
	isbn = {14714914 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868212770\&partnerID=40\&md5=93ff561cb7d1c818f78a88ec1555181e},
	author = {Bordeira-Carri{\c c}o, R. and P{\^e}go, A.P. and Santos, M. and Oliveira, C.}
}
@article {2820,
	title = {Dissection and reconstruction of the extracellular matrix - a cardiac regenerative niche},
	journal = {Journal of Tissue Engineering and Regenerative Medicine},
	volume = {6669 LNCS6},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: Scopus},
	month = {2012},
	pages = {110 - 15.P24},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=58726bd7fc58cf587a13af9e3ba0c151},
	author = {Nascimento D. and Silva A. and Oliveira MJ. and Barbosa MA. and Pinto-do-{\'O} P.}
}
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},
	month = {2012///},
	pages = {2981 - 2989},
	abstract = {E-cadherin plays a major role in cell-cell adhesion and inactivating germline mutations in its encoding gene predispose to hereditary diffuse gastric cancer. Evidence indicates that aside from its recognized role in early tumourigenesis, E-cadherin is also pivotal for tumour progression, including invasion and metastization. Herein, we discuss E-cadherin alterations found in a cancer context, associated cellular effects and signalling pathways, and we raise new key questions that will impact in the management of GC patients and families. {\textcopyright} 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.},
	keywords = {Adhesion, Cadherins, cancer growth, cancer invasion, cancer patient, cancer susceptibility, cell adhesion, complex formation, conference paper, E-cadherin, embryo development, endocytosis, epigenetics, epithelial mesenchymal transition, Gastric cancer, gene mutation, gene silencing, genetic susceptibility, Germ-Line Mutation, HDGC, Helicobacter infection, Helicobacter Infections, Helicobacter pylori, histone deacetylase inhibitor, human, Humans, Invasion, metastasis, molecular pathology, nonhuman, priority journal, protein analysis, protein depletion, protein expression, protein function, protein structure, regulatory mechanism, signal transduction, Signalling, stomach cancer, stomach carcinogenesis, Stomach Neoplasms, Tumour, uvomorulin},
	isbn = {00145793 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864922992\&partnerID=40\&md5=455ae054dc6c8f95dc9e81d77fec1ad9},
	author = {Carneiro, P. and Fernandes, M.S. and Figueiredo, J. and Caldeira, J. and Carvalho, J. and Pinheiro, H. and Leite, M. and Melo, S. and Oliveira, P. and Sim{\~o}es-Correia, J. and Oliveira, M.J. and Carneiro, F. and Figueiredo, C. and Paredes, J. and Oliveira, C. and Seruca, R.}
}
@booklet {2179,
	title = {Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII                    7-10)
},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.10.029Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 41email: iamaral@ineb.up.pt},
	month = {2012///},
	abstract = {The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4\% and 15\%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII                        7-10). Immobilized rhFNIII 
                        7-10 was characterized in terms of amount ( 
                        125I-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII 
                        7-10 with rhFNIII 
                        7-10 concentration, and, for the same concentration, higher amounts of rhFNIII 
                        7-10 on DA 4\% compared with DA 15\%. Moreover, rhFNIII 
                        7-10 concentrations as low as 5 and 20 μg ml 
},
	keywords = {Protein conformation, Protein radiolabelling, Spinal cord injury, Surface grafting, Three-dimensional scaffolds},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868680582\&partnerID=40\&md5=6599fd2565f4511b94e944978afc8073},
	author = {Amaral, I.F. and Neiva, I. and Ferreira da Silva, F. and Sousa, S.R. and Piloto, A.M. and Lopes, C.D.F. and Barbosa, M.A. and Kirkpatrick, C.J. and P{\^e}go, A.P.}
}
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	year = {2012},
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},
	month = {2012///},
	pages = {297 - 311},
	abstract = {E-cadherin and P-cadherin are major contributors to cell-cell adhesion in epithelial tissues, playing pivotal roles in important morphogenetic and differentiation processes during development, and in maintaining integrity and homeostasis in adult tissues. It is now generally accepted that alterations in these two molecules are observed during tumour progression of most carcinomas. Genetic or epigenetic alterations in E- and P-cadherin-encoding genes (CDH1 and CDH3, respectively), or alterations in their proteins expression, often result in tissue disorder, cellular de-differentiation, increased invasiveness of tumour cells and ultimately in metastasis. In this review, we will discuss the major properties of E- and P-cadherin molecules, its regulation in normal tissue, and their alterations and role in cancer, with a specific focus on gastric and breast cancer models. {\textcopyright} 2012 Elsevier B.V.},
	keywords = {antineoplastic activity, antineoplastic agent, Breast, breast cancer, Cancer, cancer chemotherapy, cancer model, cancer survival, drug effect, E-cadherin, Gastric, gene expression regulation, gene function, gene location, gene overexpression, gene structure, genetic association, genetic code, Helicobacter infection, Hereditary diffuse gastric cancer, human, in vivo study, loss of function mutation, lymph node metastasis, malignant neoplastic disease, molecular dynamics, molecular pathology, molecularly targeted therapy, nonhuman, P cadherin, P cadherin gene, P-cadherin, pf 03732010, priority journal, protein expression, protein function, protein inhibitor, protein processing, review, signal transduction, stomach cancer, transcription regulation, tumor gene, unclassified drug, uvomorulin, uvomorulin gene, vasculotropin inhibitor},
	isbn = {0304419X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861800037\&partnerID=40\&md5=2d52be63f4168a561b1bda1315e1f429},
	author = {Paredes, J. and Figueiredo, J. and Albergaria, A. and Oliveira, P. and Carvalho, J. and Ribeiro, A.S. and Caldeira, J. and Costa, A.M. and Sim{\~o}es-Correia, J. and Oliveira, M.J. and Pinheiro, H. and Pinho, S.S. and Mateus, R. and Reis, C.A. and Leite, M. and Fernandes, M.S. and Schmitt, F. and Carneiro, F. and Figueiredo, C. and Oliveira, C. and Seruca, R.}
}
@article {2191,
	title = {(Gd,Yb,Tb)PO                    4 up-conversion nanocrystals for bimodal luminescence-MR imaging
},
	journal = {NanoscaleNanoscale},
	volume = {4},
	year = {2012},
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},
	month = {2012///},
	pages = {5154 - 5162},
	abstract = {Up-conversion (Gd,Yb,Tb)PO                        4 materials and their potential for bimodal imaging have received little attention in the literature. Herein, we report the first study on the up-conversion emission of (Gd,Yb,Tb)PO 
                        4 nanocrystals synthesized via a hydrothermal method at 150{\textdegree}C. These materials exhibit ultraviolet, blue and green up-conversion emissions upon excitation with a 980 nm continuous wave laser diode. The intensity of the blue-emission band at 479 nm, ascribed to the cooperative up-conversion emission of a pair of excited Yb 
                        3+ ions, depends on the Yb 
                        3+/Tb 
                        3+ concentration ratio, calcination temperature and particle size. Strong green up-conversion emission of Tb 
                        3+ is observed at 543 nm for the 
                        5D 
                        4 {\textrightarrow} 
                        7F 
                        5 transition. Relaxometry measurements reveal that the nanocrystals are efficient T 
                        2-weighted (negative) contrast agents which, combined with visible-light emission generated by infrared excitation, affords them considerable potential for being used in bimodal, photoluminescence-magnetic resonance, imaging. {\textcopyright} 2012 The Royal Society of Chemistry.
},
	keywords = {Blue-emission, Calcination, Calcination temperature, Concentration ratio, Continuous wave lasers, Contrast agent, Gadolinium, Hydrothermal methods, Infrared excitation, Magnetic resonance, Magnetic resonance imaging, Nanocrystals, Relaxometry, Up-conversion, Up-conversion emission, Visible light, Ytterbium},
	isbn = {20403364 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864473419\&partnerID=40\&md5=c7f84b016cee67b4bf5fc3781f6b9861},
	author = {Debasu, M.L. and Ananias, D. and Pinho, S.L.C. and Geraldes, C.F.G.C. and Carlos, L.D. and Rocha, J.}
}
@booklet {2185,
	title = {The importance of antimicrobial peptides and their potential for therapeutic use in ophthalmology},
	howpublished = {International Journal of Antimicrobial AgentsInt. J. Antimicrob. Agents},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in PressCODEN: IAAGEdoi: 10.1016/j.ijantimicag.2012.07.020Language of Original Document: EnglishCorrespondence Address: Pintado, M.; CBQF-College of Biotechnology, Catholic University of Portugal, Rua Dr Ant{\'o}nio Bernardino deemail: mpintado@porto.ucp.pt},
	month = {2012///},
	abstract = {The reduced effectiveness of some of the most important antibiotics owing to increasing resistance of microorganisms as well as the absence of new classes of antimicrobial agents have been concerning researchers and clinicians in recent years. Thus, the development and approval of new compounds for clinical applications is of great importance. Among these compounds, antimicrobial peptides (AMPs) appear to be excellent candidates for the development of novel antimicrobial agents. Some AMPs and antimicrobial proteins have been shown to be active against relevant pathogens in ocular infections as well as in biofilm eradication from contact lenses. Thus, they are considered promising in the prevention and management of ocular diseases. This review summarises the main classes and characteristics of AMPs and antimicrobial proteins, in particular those found in ocular structures and fluids. Some AMPs with activity against ocular pathogens and their potential as therapeutic agents to treat and prevent ocular infections are emphasised. {\textcopyright} 2012.},
	keywords = {Antimicrobial peptide, Antimicrobial protein, Ocular infection, Ophthalmology},
	isbn = {09248579 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867901653\&partnerID=40\&md5=c1e455bf510a619011a259e85dcb6156},
	author = {Silva, N.C. and Sarmento, B. and Pintado, M.}
}
@article {2178,
	title = {Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration},
	journal = {Dental MaterialsDent. Mater.},
	volume = {28},
	year = {2012},
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},
	month = {2012///},
	pages = {1250 - 1260},
	abstract = {Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO                        2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 
                        2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 
                        2 surfaces with 0\%, 1\% and 5\% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 
                        2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. {\textcopyright} 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
},
	keywords = {Adhesion, AFM, Aggregates, Bacteria, Bacterial adhesion, Bioactive nanoparticles, Biological characterization, Biological materials, Biological response, Biomaterials, Cell culture, Contact angle, Dental prostheses, Flowcharting, FTIR/ATR, Guided tissue regeneration, Lithography, Mesenchymal stem cell, Micro patterning, Micro-texture, Microaggregates, Micropatterned, Micropatterned surface, Micropatterning, Nano-HA, Nano-hydroxyapatite, S. mutans, SEM/EDS, Silica, Silica thin films, Soft-lithography, Sol-gel, Sol-gel process, Sol-gels, Streptococcus mutans, Surface modification techniques, Surfaces, Thin films},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867841771\&partnerID=40\&md5=f33477063f16e99361687470c94d7289},
	author = {Carvalho, A. and Pelaez-Vargas, A. and Gallego-Perez, D. and Grenho, L. and Fernandes, M.H. and De Aza, A.H. and Ferraz, M.P. and Hansford, D.J. and Monteiro, F.J.}
}
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},
	month = {2012///},
	pages = {1167 - 1180},
	abstract = {Aim: Innovative solutions in the development of drug delivery systems targeting the nerve tissue are awaited. In this regard, a novel system for the delivery of drugs to the sciatic nerve was created using nanomedical principles. Materials \& methods: Chitosan was the vehicle material used in the experiment. Heparin bound to growth factors has been administered to enhance peripheral nerve regeneration, and since heparin possesses the appropriate charge to be able to form nanoparticles with chitosan, it appears to be a good candidate to base this new delivery system on. Results: Maximal absorption took place throughout the extracellular matrix at day 15. No major inflammatory response was observed, indicating that this is a safe and biocompatible system for drug delivery to nerves. Sensorimotor performance and nerve regeneration of mice receiving these nanoparticles were superior as compared with controls. Conclusion: Our work demonstrates a versatile nanoparticle delivery system that successfully targets drugs {\textquoteright}in vivo to the sciatic nerve, opening novel avenues in the field of nanomedicine to the design of therapeutic strategies that enhance axonal regeneration. {\textcopyright} 2012 Future Medicine Ltd.},
	keywords = {chitosan, drug delivery system, heparin, nanomedicine, nanoparticles, peripheral nerve regeneration, sciatic nerve},
	isbn = {17435889 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868111412\&partnerID=40\&md5=5db0c76b3a921ca70565c9f3f63cf004},
	author = {Gon{\c c}alves, N.P. and Oliveira, H. and P{\^e}go, A.P. and Saraiva, M.J.}
}
@article {2797,
	title = {Osteoporotic hip fractures: Bisphosphonates sales and observed turning point in trend. A population-based retrospective study},
	year = {2012},
	month = {2012},
	publisher = {Elsevier},
	author = {Alves, Sandra Maria and Economou, Theodoros and Oliveira, Carla and Ribeiro, Ana Isabel and Neves, Nuno and Gom{\'e}z-Barrena, Enrique and de Pina, Maria de F{\'a}tima}
}
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	volume = {16},
	year = {2012},
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},
	month = {2012///},
	pages = {2564 - 2582},
	abstract = {Spinal cord injury triggers a complex set of events that lead to tissue healing without the restoration of normal function due to the poor regenerative capacity of the spinal cord. Nevertheless, current knowledge about the intrinsic regenerative ability of central nervous system axons, when in a supportive environment, has made the prospect of treating spinal cord injury a reality. Among the range of strategies under investigation, cell-based therapies offer the most promising results, due to the multifactorial roles that these cells can fulfil. However, the best cell source is still a matter of debate, as are clinical issues that include the optimal cell dose as well as the timing and route of administration. In this context, the role of biomaterials is gaining importance. These can not only act as vehicles for the administered cells but also, in the case of chronic lesions, can be used to fill the permanent cyst, thus creating a more favourable and conducive environment for axonal regeneration in addition to serving as local delivery systems of therapeutic agents to improve the regenerative milieu. Some of the candidate molecules for the future are discussed in view of the knowledge derived from studying the mechanisms that facilitate the intrinsic regenerative capacity of central nervous system neurons. The future challenge for the multidisciplinary teams working in the field is to translate the knowledge acquired in basic research into effective combinatorial therapies to be applied in the clinic. {\textcopyright} 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.},
	keywords = {Biomaterials, Cell therapies, Clinical trials, Conditioning lesion, Gene therapy, Growth factors, Spinal cord injury, Stem cells},
	isbn = {15821838 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868098934\&partnerID=40\&md5=adb61a7d32054dc21aa4d733bc31427f},
	author = {P{\^e}go, A.P. and Kubinova, S. and Cizkova, D. and Vanicky, I. and Mar, F.M. and Sousa, M.M. and Sykova, E.}
}
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},
	month = {2012///},
	pages = {233 - 240},
	abstract = {Background/Aims: The microenvironment produces important factors that are crucial to prostate cancer (PCa) progression. However, the extent to which the cancer cells stimulate periprostatic adipose tissue (PPAT) to produce these proteins is largely unknown. Our purpose was to determine whether PCa cell-derived factors influence PPAT metabolic activity. Methods: Primary cultures of human PPAT samples from PCa patients (adipose tissue organotypic explants and primary stromal vascular fraction, SVF) were stimulated with conditioned medium (CM) collected from prostate carcinoma (PC3) cells. Cultures without CM were used as control. We used multiplex analysis and ELISA for protein quantification, qPCR to determine mitochondrial DNA (mtDNA) copy number and zymography for matrix metalloproteinase activity, in order to evaluate the response of adipose tissue explants and SVFs to PC3 CM. Results: Stimulation of PPAT explants with PCa PC3 CM induced adipokines associated with cancer progression (osteopontin, tumoral necrosis factor alpha and interleukin-6) and reduced the expression of the protective adipokine adiponectin. Notably, osteopontin protein expression was 13-fold upregulated. Matrix metalloproteinase 9 activity and mitochondrial DNA copy number were higher after stimulation with cancer CM. Stromovascular cells from PPAT in culture were not influenced by tumor-derived factors. Conclusion: The modulation of adipokine expression by tumor CM indicates the pervasive extent to which tumor cells command PPAT to produce factors favorable to their aggressiveness. {\textcopyright} 2012 S. Karger AG, Basel.},
	keywords = {Adipokines, adiponectin, adipose tissue, adult, article, cancer cell, cancer growth, Cells, Cultured, clinical article, controlled study, Culture Media, Conditioned, culture medium, Cytokines, DNA, Mitochondrial, enzyme activity, enzyme linked immunosorbent assay, explant, gelatinase B, human, human cell, human tissue, Humans, interleukin 6, Interleukin-6, male, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Middle Aged, mitochondrial DNA, Osteopontin, Periprostatic adipose tissue, priority journal, Prostate cancer, Prostatic Neoplasms, protein expression, protein induction, protein secretion, quantitative analysis, stroma cell, tissue metabolism, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha},
	isbn = {10158987 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859829669\&partnerID=40\&md5=a76be7e5c0037e77140c1fc9aca48a5a},
	author = {Ribeiro, R.J.T. and Monteiro, C.P.D. and Cunha, V.F.P.M. and Azevedo, A.S.M. and Oliveira, M.J. and Monteiro, R. and Fraga, A.M. and Pr{\'\i}ncipe, P. and Lobato, C. and Lobo, F. and Morais, A. and Silva, V. and Sanches-Magalh{\~a}es, J. and Oliveira, J. and Guimar{\~a}es, J.T. and Lopes, C.M.S. and Medeiros, R.M.}
}
@article {2241,
	title = {The Arabidopsis D-Type Cyclin CYCD2;1 and the Inhibitor ICK2/KRP2 Modulate Auxin-Induced Lateral Root Formation},
	journal = {Plant CellPlant Cell},
	volume = {23},
	year = {2011},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: ScopusCODEN: PLCEEdoi: 10.1105/tpc.110.080002PubMed ID: 21357490Language of Original Document: EnglishCorrespondence Address: Murray, J. A. H.; Cardiff School of Biosciences, Cardiff University, CF10 3AX Cardiff, United Kingdom; email: murrayja1@cardiff.ac.ukChemicals/CAS: proteasome, 140879-24-9; ATP dependent 26S protease, 3.4.99.-; Arabidopsis Proteins; Cell Cycle Proteins; CycD2 protein, Arabidopsis; Cyclins; ICK2 protein, Arabidopsis; Indoleacetic Acids; Proteasome Endopeptidase Complex, 3.4.25.1References: Achard, P., Gusti, A., Cheminant, S., Alioua, M., Dhondt, S., Coppens, F., Beemster, G.T., Genschik, P., Gibberellin signaling controls cell proliferation rate in Arabidopsis (2009) Curr. Biol., 19, pp. 1188-1193;Alonso, J.M., Genome-wide insertional mutagenesis of Arabidopsis thaliana (2003) Science, 301, pp. 653-657; 
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},
	month = {2011///},
	pages = {641 - 660},
	abstract = {The integration of cell division in root growth and development requires mediation of developmental and physiological signals through regulation of cyclin-dependent kinase activity. Cells within the pericycle form de novo lateral root meristems, and D-type cyclins (CYCD), as regulators of the G                        1-to-S phase cell cycle transition, are anticipated to play a role. Here, we show that the D-type cyclin protein CYCD2;1 is nuclear in Arabidopsis thaliana root cells, with the highest concentration in apical and lateral meristems. Loss of CYCD2;1 has a marginal effect on unstimulated lateral root density, but CYCD2;1 is rate-limiting for the response to low levels of exogenous auxin. However, while CYCD2;1 expression requires sucrose, it does not respond to auxin. The protein Inhibitor-Interactor of CDK/Kip Related Protein2 (ICK2/KRP2), which interacts with CYCD2;1, inhibits lateral root formation, and ick2/krp2 mutants show increased lateral root density. ICK2/ KRP2 can modulate the nuclear levels of CYCD2;1, and since auxin reduces ICK2/KRP2 protein levels, it affects both activity and cellular distribution of CYCD2;1. Hence, as ICK2/KRP2 levels decrease, the increase in lateral root density depends on CYCD2;1, irrespective of ICK2/CYCD2;1 nuclear localization. We propose that ICK2/KRP2 restrains root ramification by maintaining CYCD2;1 inactive and that this modulates pericycle responses to auxin fluctuations. {\textcopyright} 2011 American Society of Plant Biologists.
},
	keywords = {Arabidopsis, Arabidopsis protein, Arabidopsis Proteins, Arabidopsis thaliana, article, ATP dependent 26S protease, cell cycle, cell cycle protein, Cell Cycle Proteins, cell nucleus, CycD2 protein, Arabidopsis, cycline, Cyclins, gene expression regulation, Gene Expression Regulation, Plant, genetics, growth, development and aging, ICK2 protein, Arabidopsis, indoleacetic acid derivative, Indoleacetic Acids, metabolism, mutation, plant root, Plant roots, proteasome, Proteasome Endopeptidase Complex},
	isbn = {10404651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953123085\&partnerID=40\&md5=f1355f64db2bcf9f2d881104f23005eb},
	author = {Sanz, L. and Dewitte, W. and Forzani, C. and Patell, F. and Nieuwland, J. and Wen, B. and Quelhas, P. and de Jager, S. and Titmus, C. and Campilho, A. and Ren, H. and Estelle, M. and Wang, H. and Murray, J.A.H.}
}
@book {2224,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6669 LNCS},
	year = {2011},
	note = {Conference code: 85429Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21257-4_19Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: meb09026@fe.up.ptReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Alattar, M., Osman, N., Fahmy, A., Myocardial segmentation using constrained multi-seeded region growing (2010) LNCS, 6112, pp. 89-98. , Campilho, A., Kamel, M. (eds.) ICIAR 2010 Springer, Heidelberg; 
Wu, Q., Merchant, F., Castleman, K., (1996) Microscope Image Processing, , ch. 7. Elsevier, Amsterdam; 
Gonzalez, R., Woods, R., Eddins, S., (2004) Digital Image Processing Using MATLAB, , ch. 9. Pearson Education, London; 
Sharma, N., Aggarwal, L., Automated medical image segmentation techniques (2010) Journal of Medical Physics, 35 (1), pp. 3-14; 
Pham, D., Xu, C., A survey of current methods in medical image segmentation (1998) Annual Review of Biomedical Engineering, 2, pp. 315-338; 
Hamarneh, G., Li, X., Watershed segmentation using prior shape and appearance knowledge (2009) Image and Vision Comp, 27 (1-2), pp. 59-68; 
Khadir, S., Ahamed, R., Moving toward region-based image segmentation techniques: A study (2009) Journal of Theoretical and Applied Information Technology, 5 (1), pp. 1-7; 
Ahmed, M., Mohamad, D., Segmentation of brain mr images for tumor extraction by combining kmeans clustering and perona-malik anisotropic diffusion model (2010) International Journal of Image Processing, 2 (1), pp. 1-8; 
Mayer, A., Greenspan, H., An adaptive mean-shift framework for MRI brain segmentation (2009) IEEE Trans. on Medical Imaging, 28 (8), pp. 1-12; 
Dabov, K., Foi, A., Katkovnik, V., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 1-16; 
Otsu, N., A threshold selection method from gray level histograms (1979) IEEE Trans. Systems, Man and Cybernetics, 9 (1), pp. 62-66; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Computers in Biology and Medicine, 39 (9), pp. 1-9; 
Bleau, A., Leon, J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370
},
	month = {2011///},
	pages = {151 - 158},
	address = {Las Palmas de Gran Canaria},
	abstract = {Rodent models of myocardial infarction (MI) have been extensively used in biomedical research towards the implementation of novel regenerative therapies. Permanent ligation of the left anterior descending (LAD) coronary artery is a commonly used method for inducing MI both in rat and mouse. Post-mortem evaluation of the heart, particularly the MI extension assessment performed on histological sections, is a critical parameter for this experimental setting. MI extension, which is defined as the percentage of the left ventricle affected by the coronary occlusion, has to be estimated by identifying the infarcted- and the normal-tissue in each section. However, because it is a manual procedure it is time-consuming, arduous and prone to bias. Herein, we introduce semi-automatic and automatic approaches to perform segmentation which is then used to obtain the infarct extension measurement. Experimental validation is performed comparing the proposed approaches with manual annotation and a total error not exceeding 8\% is reported in all cases. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Extension evaluations, Image analysis, Image segmentation, Infarct extension evaluation, k-means, Mammals, meanshift, otsu, Pattern recognition, region growing, Tissue, watershed},
	isbn = {03029743 (ISSN); 9783642212567 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=360bb21a8c1c3a5fdd394c647fcadbbc},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@book {2818,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)},
	volume = {6669 LNCS},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: Scopus},
	month = {2011///},
	pages = {151 - 158},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=58726bd7fc58cf587a13af9e3ba0c151},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@article {2234,
	title = {Bone marrow-derived endothelial progenitors expressing delta-like 4 (Dll4) regulate tumor angiogenesis},
	journal = {PLoS ONEPLoS ONE},
	volume = {6},
	year = {2011},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusArt. No.: e18323doi: 10.1371/journal.pone.0018323Language of Original Document: EnglishCorrespondence Address: Dias, S.; Angiogenesis Laboratory, CIPM/Portuguese Institute of Oncology, Lisbon, Portugal; email: sergidias@ipolisboa.min-saude.ptChemicals/CAS: fibronectin, 86088-83-7; vasculotropin, 127464-60-2; DLL4 protein, mouse; Intracellular Signaling Peptides and Proteins; Membrane ProteinsReferences: Asahara, T., Masuda, H., Takahashi, T., Kalka, C., Pastore, C., Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization (1999) Circ Res, 85, pp. 221-228;Rafii, S., Lyden, D., Benezra, R., Hattori, K., Heissig, B., Vascular and haematopoietic stem cells: Novel targets for anti-angiogenesis therapy? (2002) Nat Rev Cancer, 2, pp. 826-835. , Review; 
Lyden, D., Hattori, K., Dias, S., Costa, C., Blaikie, P., Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth (2001) Nat Med, 7, pp. 1194-1201; 
Larrivee, B., Niessen, K., Pollet, I., Corbel, S.Y., Long, M., Minimal contribution of marrow-derived endothelial precursors to tumor vasculature (2005) J Immunol, 175, pp. 2890-2899; 
Peters, B.A., Diaz, L.A., Polyak, K., Meszler, L., Romans, K., Contribution of bone marrow-derived endothelial cells to human tumor vasculature (2005) Nat Med, 11, pp. 261-262; 
Pasquier, E., Dias, S., Endothelial progenitor cells: Hope beyond controversy (2010) Curr Cancer Drug Targets, 10 (8), pp. 914-921; 
Dudley, A.C., Udagawa, T., Melero-Martin, J.M., Shih, S.C., Curatolo, A., Bone marrow is a reservoir for proangiogenic myelomonocytic cells but not endothelial cells in spontaneous tumors (2010) Blood, 116 (17), pp. 2267-2271; 
Krebs, L.T., Xue, Y., Norton, C.R., Shutter, J.R., Maguire, M., Notch signaling is essential for vascular morphogenesis in mice (2000) Genes Dev, 14, pp. 1343-1352; 
Uyttendaele, H., Ho, J., Rossant, J., Kitajewski, J., Vascular patterning defects associated with expression of activated Notch4 in embryonic endothelium (2001) Proc Natl Acad Sci U S A, 98, pp. 5643-5648; 
Duarte, A., Hirashima, M., Benedito, R., Trindade, A., Diniz, P., Dosage-sensitive requirement for mouse Dll4 in artery development (2004) Genes Dev, 18, pp. 2474-2478; 
Gale, N.W., Dominguez, M.G., Noguera, I., Pan, L., Hughes, V., Haploinsufficiency of delta-like 4 ligand results in embryonic lethality due to major defects in arterial and vascular development (2004) Proc Natl Acad Sci U S A, 101, pp. 15949-15954; 
Carmeliet, P., Ferreira, V., Breier, G., Pollefeyt, S., Kieckens, L., Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele (1996) Nature, 380, pp. 435-439; 
Mailhos, C., Modlich, U., Lewis, J., Harris, A., Bicknell, R., Delta4, an endothelial specific notch ligand expressed at sites of physiological and tumor angiogenesis (2001) Differentiation, 69, pp. 135-144; 
Oliner, J., Min, H., Leal, J., Yu, D., Rao, S., Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2 (2004) Cancer Cell, 6, pp. 507-516; 
Patel, N.S., Li, J.L., Generali, D., Poulsom, R., Cranston, D.W., Up-regulation of delta-like 4 ligand in human tumor vasculature and the role of basal expression in endothelial cell function (2005) Cancer Res, 65, pp. 8690-8697; 
Williams, C.K., Li, J.L., Murga, M., Harris, A.L., Tosato, G., Up-regulation of the Notch ligand Delta-like 4 inhibits VEGF-induced endothelial cell function (2006) Blood, 107, pp. 931-939; 
Caiado, F., Real, C., Carvalho, T., Dias, S., Notch pathway modulation on bone marrow-derived vascular precursor cells regulates their angiogenic and wound healing potential (2008) PLoS One, 3, pp. e3752; 
Escudier, B., Eisen, T., Stadler, W.M., Szczylik, C., Oudard, S., Sorafenib in advanced clear-cell renal-cell carcinoma (2007) N Engl J Med, 356, pp. 125-134; 
Llovet, J.M., Ricci, S., Mazzaferro, V., Hilgard, P., Gane, E., Sorafenib in advanced hepatocellular carcinoma (2008) N Engl J Med, 359, pp. 378-390; 
Motzer, R.J., Bukowski, R.M., Targeted therapy for metastatic renal cell carcinoma (2006) J Clin Oncol, 24, pp. 5601-5608; 
Bergers, G., Hanahan, D., Modes of resistance to anti-angiogenic therapy (2008) Nat Rev Cancer, 8, pp. 592-603; 
Verheul, H.M., Pinedo, H.M., Possible molecular mechanisms involved in the toxicity of angiogenesis inhibition (2007) Nat Rev Cancer, 7, pp. 475-485; 
Noguera-Troise, I., Daly, C., Papadopoulos, N.J., Coetzee, S., Boland, P., Blockade of Dll4 inhibits tumour growth by promoting non-productive angiogenesis (2006) Nature, 444, pp. 1032-1037; 
Ridgway, J., Zhang, G., Wu, Y., Stawicki, S., Liang, W.C., Inhibition of Dll4 signalling inhibits tumour growth by deregulating angiogenesis (2006) Nature, 444, pp. 1083-1087; 
Moore, M.A., Hattori, K., Heissig, B., Shieh, J.H., Dias, S., Mobilization of endothelial and hematopoietic stem and progenitor cells by adenovector-mediated elevation of serum levels of SDF-1, VEGF, and angiopoietin-1 (2001) Ann N Y Acad Sci, 938, pp. 36-45. , discussion 45-37; 
Yamaguchi, J., Kusano, K.F., Masuo, O., Kawamoto, A., Silver, M., Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization (2003) Circulation, 107, pp. 1322-1328; 
Leslie, J.D., Ariza-McNaughton, L., Bermange, A.L., McAdow, R., Johnson, S.L., Endothelial signalling by the Notch ligand Delta-like 4 restricts angiogenesis (2007) Development, 134, pp. 839-844; 
Trindade, A., Kumar, S.R., Scehnet, J.S., Lopes-da-Costa, L., Becker, J., Overexpression of delta-like 4 induces arterialization and attenuates vessel formation in developing mouse embryos (2008) Blood, 112, pp. 1720-1729; 
Huang, M.T., Mason, J.C., Birdsey, G.M., Amsellem, V., Gerwin, N., Endothelial intercellular adhesion molecule (ICAM)-2 regulates angiogenesis (2005) Blood, 106, pp. 1636-1643; 
Deutsch, U., Schlaeger, T.M., Dehouck, B., Doring, A., Tauber, S., Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice (2008) Exp Cell Res, 314, pp. 1202-1216; 
Dias, S., Thomas, H., Balkwill, F., Multiple molecular and cellular changes associated with tumour stasis and regression during IL-12 therapy of a murine breast cancer model (1998) Int J Cancer, 75 (1), pp. 151-157; 
Scott, K.A., Holdsworth, H., Balkwill, F.R., Dias, S., Exploiting changes in the tumour microenvironment with sequential cytokine and matrix metalloprotease inhibitor treatment in a murine breast cancer model (2000) Br J Cancer, 83 (11), pp. 1538-1543
},
	month = {2011///},
	abstract = {Neo-blood vessel growth (angiogenesis), which may involve the activation of pre-existing endothelial cells (EC) and/or the recruitment of bone marrow-derived vascular precursor cells (BM-VPC), is essential for tumor growth. Molecularly, besides the well established roles for Vascular endothelial growth factor (VEGF), recent findings show the Notch signalling pathway, in particular the ligand Delta-like 4 (Dll4), is also essential for adequate tumor angiogenesis; Dll4 inhibition results in impaired, non-functional, angiogenesis and reduced tumor growth. However, the role of BM-VPC in the setting of Notch pathway modulation was not addressed and is the subject of the present report. Here we show that SDF-1 and VEGF, which are produced by tumors, increase Dll4 expression on recruited BM-VPC. Mechanistically, BM-VPC activated, in a Dll4-dependent manner, a transcriptional program on mature EC suggestive of EC activation and stabilization. BM-VPC induced ICAM-2 and Fibronectin expression on EC, an effect that was blocked by a Dll4-specific neutralizing antibody. In vivo, transplantation of BM-VPC with decreased Dll4 into tumor-bearing mice resulted in the formation of microvessels with decreased pericyte coverage and reduced fibronectin expression. Consequently, transplantation of BM-VPC with decreased Dll4 resulted in impaired tumor angiogenesis, increased tumor hypoxia and apoptosis, and decreased tumor growth. Taken together, our data suggests that Dll4 expression by BM-VPC affects their communication with tumor vessel endothelial cells, thereby modulating tumor angiogenesis by affecting vascular stability. {\textcopyright} 2011 Real et al.},
	keywords = {angiogenesis, animal, animal cell, animal tissue, Animals, apoptosis, article, blood vessel, Blood Vessels, bone marrow, bone marrow cell, Bone Marrow Cells, cell activation, Cell Line, Tumor, cell proliferation, controlled study, cytology, delta like 4 protein, DLL4 protein, mouse, Endothelial Cells, endothelium cell, Female, Fibronectin, gene expression, gene expression regulation, genetics, human, human cell, Humans, hypoxia, in vitro study, in vivo study, intercellular adhesion molecule 2, Intracellular Signaling Peptides and Proteins, membrane protein, Membrane Proteins, metabolism, Mice, mouse, Mus, neoplasm, Neoplasms, neovascularization (pathology), Neovascularization, Pathologic, neutralizing antibody, nonhuman, Notch receptor, Pathology, pericyte, protein expression, signal peptide, solid tumor, stem cell, stem cell transplantation, Stem cells, stromal cell derived factor 1, tumor cell line, tumor growth, unclassified drug, vascularization, vasculotropin},
	isbn = {19326203 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953685607\&partnerID=40\&md5=60a33b7d91c1502050bc560303255881},
	author = {Real, C. and Rem{\'e}dio, L. and Caiado, F. and Igreja, C. and Borges, C. and Trindade, A. and Pinto-Do-{\'O}, P. and Yagita, H. and Duarte, A. and Dias, S.}
}
@article {2233,
	title = {The effect of slurry preparation methods on biaxial flexural strength of dental porcelain},
	journal = {Journal of Prosthetic DentistryJ. Prosthet. Dent.},
	volume = {105},
	year = {2011},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JPDEAdoi: 10.1016/S0022-3913(11)60058-9PubMed ID: 21530756Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; Institute of Biomedical Engineering, Rua do Campo Alegre, 283, 4150-180 Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: Complex Mixtures; Dental Porcelain, 12001-21-7References: The Glossary of Prosthodontic Terms (2005) J Prosthet Dent, 94, pp. 10-92;(2008) Dentistry - Ceramic Materials, , ISO 6872 Geneva, Switzerland: International Organization for Standards; 
Sadowsky, S.J., An overview of treatment considerations for esthetic restorations: A review of the literature (2006) Journal of Prosthetic Dentistry, 96 (6), pp. 433-442. , DOI 10.1016/j.prosdent.2006.09.018, PII S0022391306004756; 
Messer, R.L., Lockwood, P.E., Wataha, J.C., Lewis, J.B., Norris, S., Bouillaguet, S., In vitro cytotoxicity of traditional versus contemporary dental ceramics (2003) J Prosthet Dent, 90, pp. 452-458; 
Oh, W.-S., DeLong, R., Anusavice, K.J., Factors affecting enamel and ceramic wear: A literature review (2002) Journal of Prosthetic Dentistry, 87 (4), pp. 451-459. , DOI 10.1067/mpr.2002.123851; 
Schuh, C., Kinast, E.J., Mezzomo, E., Kapczinski, M.P., Effect of glazed and polished surface finishes on the friction coefficient of two low-fusing ceramics (2005) Journal of Prosthetic Dentistry, 93 (3), pp. 245-252. , DOI 10.1016/j.prosdent.2004.12.010; 
Anusavice, K.J., Degradability of dental ceramics (1992) Adv Dent Res, 6, pp. 82-89; 
Kelly, J.R., Nishimura, I., Campbell, S.D., Ceramics in dentistry: Historical roots and current perspectives (1996) Journal of Prosthetic Dentistry, 75 (1), pp. 18-32; 
Rosenblum, M.A., Schulman, A., A Review of All-Ceramic Restorations (1997) Journal of the American Dental Association, 128 (3), pp. 297-307; 
Jung, Y.-G., Wuttiphan, S., Peterson, I.M., Lawn, B.R., Damage modes in dental layer structures (1999) Journal of Dental Research, 78 (4), pp. 887-897; 
Cesar, P.F., Yoshimura, H.N., Miranda Jr., W.G., Okada, C.Y., Correlation between fracture toughness and leucite content in dental porcelains (2005) Journal of Dentistry, 33 (9), pp. 721-729. , DOI 10.1016/j.jdent.2005.02.001, PII S0300571205000503; 
Griggs, J.A., Kishen, A., Le, K.N., Mechanism of strength increase for a hydrothermal porcelain (2003) Dent Mater, 19, pp. 625-631; 
Oilo, G., Flexural strength and internal defects of some dental porcelains (1988) Acta Odontol Scand, 46, pp. 313-322; 
Sinmazisik, G., Ove{\c c}oglu, M.L., Physical properties and microstructural characterization of dental porcelains mixed with distilled water and modeling liquid (2006) Dent Mater, 22, pp. 735-745; 
Zhang, Y., Griggs, J.A., Benham, A.W., Influence of powder/liquid mixing ratio on porosity and translucency of dental porcelains (2004) Journal of Prosthetic Dentistry, 91 (2), pp. 128-135. , DOI 10.1016/j.prosdent.2003.10.014; 
Griggs, J.A., Thompson, J.Y., Anusavice, K.J., Effects of flaw size and auto-glaze treatment on porcelain strength (1996) Journal of Dental Research, 75 (6), pp. 1414-1417; 
Dos Santos, J.G., Fonseca, R.G., Adabo, G.L., Dos Santos Cruz, C.A., Shear bond strength of metal-ceramic repair systems (2006) J Prosthet Dent, 96, pp. 165-173; 
Galiatsatos, A.A., An indirect repair technique for fractured metal-ceramic restorations: A clinical report (2005) Journal of Prosthetic Dentistry, 93 (4), pp. 321-323. , DOI 10.1016/j.prosdent.2004.12.018; 
Fleming, G.J., Shaini, F.J., Marquis, P.M., An assessment of the influence of mixing induced variability on the bi-axial flexure strength of dentine porcelain discs and the implications for laboratory testing of porcelain specimens (2000) Dent Mater, 16, pp. 114-119; 
Palin, W.M., Fleming, G.J.P., Marquis, P.M., An evaluation of the technique sensitivity of a hydrothermal low-fusing dental ceramic (2001) Journal of Dentistry, 29 (6), pp. 443-449. , DOI 10.1016/S0300-5712(01)00039-2, PII S0300571201000392; 
Zeng, K., Oden, A., Rowcliffe, D., Flexure tests on dental ceramics (1996) International Journal of Prosthodontics, 9 (5), pp. 434-439; 
Cattell, M.J., Clarke, R.L., Lynch, E.J.R., The transverse strength, reliability and microstructural features of four dental ceramics - Part I (1997) Journal of Dentistry, 25 (5), pp. 399-407. , PII S0300571296000589; 
Gill, J., Methods and results in condensation of dental porcelain (1932) J Am Dent Assoc, 19, pp. 1147-1154; 
Anusavice, K.J., Hojjatie, B., Stress distribution in metal-ceramic crowns with a facial porcelain margin (1987) J Dent Res, 66, pp. 1493-1498; 
Fleming, G.J.P., Shelton, R.M., Marquis, P.M., The influence of clinically induced variability on the bi-axial fracture strength of aluminous core porcelain discs (1999) J Dent, 27, pp. 587-594; 
Tomozawa, M., Han, W.-T., Lanford, W.A., Water entry into Silica Glass during Slow Crack Growth (1991) J Am Ceram Soc, 74, pp. 2573-2576; 
Ray, C.S., Day, D.E., Identifying internal and surface crystallization by differential thermal analysis for the glass-to-crystal transformations (1996) Thermochim Acta, 280-281, pp. 163-174; 
Mackert Jr., J.R., Williams, A.L., Ergle, J.W., Russell, C.M., Water-enhanced crystallization of leucite in dental porcelain (2000) Dent Mater, 16, pp. 426-431; 
Burgner, L.L., Weinberg, M.C., An assessment of crystal growth behavior in lithium disilicate glass (2001) J. Non-Cryst Solids, 279, pp. 28-43; 
Deubener, J., Muller, R., Behrens, H., Heide, G., Water and the glass transition temperature of silicate melts (2003) J. Non-Cryst Solids, 330, pp. 268-273; 
Seghi, R.R., Daher, T., Caputo, A., Relative flexural strength of dental restorative ceramics (1990) Dent Mater, 6, pp. 181-184; 
Quinn, J.B., Quinn, G.D., A practical and systematic review of Weibull statistics for reporting strengths of dental materials (2010) Dent Mater, 26, pp. 135-147; 
Ritter, J.E., Critique of test methods for lifetime predictions (1995) Dent Mater, 11, pp. 147-151; 
Giordano, R.A., Pelletier, L., Campbell, S., Pober, R., Flexural strength of an infused ceramic, glass ceramic, and feldspathic porcelain (1995) J Prosthet Dent, 73, pp. 411-418; 
Kelly, J.R., Perspectives on strength (1995) Dent Mater, 11, pp. 103-110; 
Griggs, J.A., Wataha, J.C., Kishen, A., Effect of hydrolyzed surface layer on the cytotoxicity and chemical resistance of a low fusing porcelain (2003) Dent Mater, 19, pp. 353-358; 
Bertolini, M.J., Zaghete, M.A., Gimenes, R., Freitas De Souza, R., Vaz, L.G., Preparation of new glass systems by the polymeric precursor method for dental applications (2004) J Non- Cryst Solids, 344, pp. 170-175; 
Tsetsekou, A., Papadopoulos, T., Adamopoulos, O., Microstructure effect on the properties of a commercial low-fusing dental porcelain (2002) Journal of Materials Science: Materials in Medicine, 13 (4), pp. 407-416. , DOI 10.1023/A:1014301021375; 
Gonzaga, C.C., Cesar, P.F., Okada, C.Y., Fredericci, C., Beneduce-Neto, F.V., Yoshimura, H.N., Mechanical properties and porosity of dental glass-ceramics hot-pressed at different temperatures (2008) Mater Res, 11, pp. 301-306; 
Pelaez, A., Garcia, C., Correa, J.C., Abad, P., Reliability Weibull analysis for structural evaluation of bioactive films obtained by sol-gel process (2004) Key Eng Mater, 254-256, pp. 431-434; 
Pham, H., (2006) Springer Handbook of Engineering Statistics, pp. 97-102. , 1st ed. London: Springer; 
Ritter, J.E., Predicting lifetimes of materials and material structures (1995) Dent Mater, 11, pp. 142-146
},
	month = {2011///},
	pages = {308 - 314},
	abstract = {Statement of problem. One-step and incremental mixing procedures are currently used to produce dental ceramic pastes. In the ceramic industry, high quality is obtained using one-step mixing, but in dentistry, the best method has not been yet determined. Purpose. The purpose of this study is to evaluate the effects of 2 mixing techniques on the biaxial flexural strength and microstructure of dental porcelain. Material and methods. Feldspathic porcelain discs (2 {\texttimes} 15 mm in diameter) were produced and divided according to the ceramic paste preparation method, powder-liquid incremental mixing group (n=50) or one-step mixing, as a control group (n=50). Specimens were tested for biaxial flexural strength and characterized using porosimetry, relative humidity, SEM/EDS, XRD, and FT-IR analyses. Statistical analysis was conducted using Weibull statistics. The Weibull modulus, characteristic strength and relative humidity were compared between groups, using Student{\textquoteright}s t-test and Mann-Whitney U test (α=.05). Results. The powder-liquid incremental mixing group showed significantly higher values (SD) of Weibull modulus (6.74 (0.70), P<.001) and characteristic strength (79.87 (2.01) MPa, P<.001) when compared to the one-step mixing group (4.94 (0.94) and 75.95 (2.61) MPa). Significantly lower mean (SD) relative humidity values (P=.009) were found for powder-liquid incremental mixing group (20\% (0.5\%)) compared to one-step mixing group (22\% (1\%)). XRD spectra showed that the one-step mixing group produced higher amounts of the amorphous phase. Conclusions. Specimens produced by the incremental mixing technique showed higher biaxial flexural strength than one-step mixing. {\textcopyright} 2011 The Editorial Council of the Journal of Prosthetic Dentistry.},
	keywords = {article, chemistry, comparative study, Complex Mixtures, Crowns, dental care, Dental Porcelain, Dental Prosthesis Design, Dental Stress Analysis, Dental Veneers, denture, drug mixture, evaluation, materials testing, pliability, tooth crown, tooth prosthesis},
	isbn = {00223913 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955604181\&partnerID=40\&md5=53c0732c254214b4a53e3222fe89f4a0},
	author = {Pelaez-Vargas, A. and Dussan, J.A. and Restrepo-Tamayo, L.F. and Paucar, C. and Ferreira, J.A. and Monteiro, F.J.}
}
@article {2240,
	title = {Gene delivery into mesenchymal stem cells: A biomimetic approach using rgd nanoclusters based on poly(amidoamine) dendrimers},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {12},
	year = {2011},
	note = {Cited By (since 1996): 10Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm1012647PubMed ID: 21210673Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; CQM-Centro de Qu{\'\i}mica da Madeira, MMRG, Universidade da Madeira, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; firefly luciferase, 61970-00-1; Dendrimers; Oligopeptides; Poly(amidoamine); Polyamines; arginyl-glycyl-aspartic acid, 99896-85-2References: Pierschbacher, M.D., Ruoslahti, E., (1984) Nature, 309, pp. 30-33;Ruoslahti, E., (1996) Annu. Rev. Cell Dev. Biol., 12, pp. 697-715; 
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},
	month = {2011///},
	pages = {472 - 481},
	abstract = {Poly(amidoamine) dendrimers (generations 5 and 6) with amine termini were conjugated with peptides containing the arginine-glycine-aspartic acid (RGD) sequence having in view their application as gene delivery vectors. The idea behind the work was to take advantage of the cationic nature of dendrimers and of the integrin targeting capabilities of the RGD motif to improve gene delivery. Dendrimers were used as scaffolds for RGD clustering and, by controlling the number of peptides (4, 8, and 16) linked to each dendrimer, it was possible to evaluate the effect of RGD density on the gene delivery process. The new vectors were characterized in respect to their ability to neutralize and compact plasmid DNA (pDNA). The complexes formed by the vectors and pDNA were studied concerning their size, zeta potential, capacity of being internalized by cells and ability of transferring genes. Transfection efficiency was analyzed, first, by using a pDNA encoding for Enhanced Green Fluorescent Protein and Firefly Luciferase and, second, by using a pDNA encoding for Bone Morphogenetic Protein-2. Gene expression in mesenchymal stem cells was enhanced using the new vectors in comparison to native dendrimers and was shown to be dependent on the electrostatic interaction established between the dendrimer moiety and the cell surface, as well as on the RGD density of nanoclusters. The use of dendrimer scaffolds for RGD cluster formation is a new approach that can be extended beyond gene delivery applications, whenever RGD clustering is important for modulating cellular responses. {\textcopyright} 2011 American Chemical Society.},
	keywords = {Amino Acid Sequence, Amino acids, animal cell, animal experiment, Animals, Arginine-glycine-aspartic acids, arginylglycylaspartic acid, article, Biomimetics, bone morphogenetic protein 2, Bone morphogenetic protein-2, Cell culture, Cell membranes, cell surface, Cell surfaces, cell survival, Cellular response, Cluster formations, Complexation, controlled study, dendrimer, Dendrimers, Electrostatic interactions, electrostatic precipitation, Encoding (symbols), Enhanced green fluorescent protein, Firefly luciferase, Functional polymers, Gene delivery, Gene delivery vectors, gene expression, gene targeting, Gene therapy, Gene transfer, Gene Transfer Techniques, gene vector, integrin, Integrins, male, Mesenchymal stem cell, Mesenchymal Stem Cells, Molecular Structure, Nanoclusters, Nanostructures, New approaches, nonhuman, Oligopeptides, Organic acids, Peptides, plasmid DNA, Plasmid DNA (pDNA), polyamidoamine, Polyamidoamine dendrimers, Polyamines, priority journal, rat, Rats, Rats, Wistar, RGD motif, Scaffolds, Stem cells, Structure-Activity Relationship, Targeting capability, Transfection efficiency, Vectors, Zeta potential},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79951658350\&partnerID=40\&md5=a3ef0b57d12de8efb52ba2bf445be126},
	author = {Pandita, D. and Santos, J.L. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2228,
	title = {Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants},
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	volume = {27},
	year = {2011},
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},
	month = {2011///},
	pages = {581 - 589},
	abstract = {Titanium implants are the gold standard in dentistry; however, problems such as gingival tarnishing and peri-implantitis have been reported. For zirconia to become a competitive alternative dental implant material, surface modification techniques that induce guided tissue growth must be developed. Objectives: To develop alternative surface modification techniques to promote guided tissue regeneration on zirconia materials, for applications in dental implantology. Methods: A methodology that combined soft lithography and sol-gel chemistry was used to obtain isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates. The materials were characterized via chemical, structural, surface morphology approaches. In vitro biological behavior was evaluated in terms of early adhesion and viability/metabolic activity of human osteoblast-like cells. Statistical analysis was conducted using one-way ANOVA/Tukey HSD post hoc test. Results: Isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates were obtained using a combined approach based on sol-gel technology and soft lithography. Micropatterned silica surfaces exhibited a biocompatible behavior, and modulated cell responses (i.e. inducing early alignment of osteoblast-like cells). After 7 d of culture, the cells fully covered the top surfaces of pillar microstructured silica films. Significance: The micropatterned silica films on zirconia showed a biocompatible response, and were capable of inducing guided osteoblastic cell adhesion, spreading and propagation. The results herein presented suggest that surface-modified ceramic implants via soft lithography and sol-gel chemistry could potentially be used to guide periodontal tissue regeneration, thus promoting tight tissue apposition, and avoiding gingival retraction and peri-implantitis. {\textcopyright} 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Adhesion, article, Biological behavior, biomaterial, biomedical and dental materials, cell adhesion, Cell culture, Cell Line, cell proliferation, Cell response, cell survival, Ceramic implants, ceramics, chemistry, Coated Materials, Biocompatible, Coatings, confocal microscopy, crystallography, Dental implant materials, Dental implantology, Dental Implants, Dental Materials, Dental prostheses, diagnostic agent, dyes, reagents, indicators, markers and buffers, Gels, Gold standards, Growth kinetics, Guided tissue regeneration, Guided Tissue Regeneration, Periodontal, heat, Hot Temperature, human, Human osteoblast-like cells, Humans, In-vitro, Indicators and Reagents, Lithography, materials testing, Micropatterned, Microscopy, Confocal, Microscopy, Electron, Scanning, osteoblast, Osteoblast-like cells, Osteoblastic cell adhesion, Osteoblasts, oxazine derivative, Oxazines, Peri-implantitis, Periodontal tissue, periodontics, phase transition, physiology, Raman spectrometry, resazurin, Scanning electron microscopy, Silica, Silica coatings, Silica film, Silica surface, silicon dioxide, Soft lithography, Sol-gel, Sol-gel chemistry, Sol-gel process, Sol-gel technology, Sol-gels, Sols, Spectrum Analysis, Raman, Stabilized zirconia, Statistical analysis, Surface modification techniques, Surface morphology, Surface Properties, surface property, Surface-modified, Surfaces, time, Time Factors, Tissue, Tissue growth, Titanium, Titanium implants, tooth implantation, Top surface, X ray diffraction, X-Ray Diffraction, xanthene derivative, Xanthenes, yttria stabilized tetragonal zirconia, Yttria stabilized zirconia, yttrium, Yttrium alloys, Zirconia, Zirconia materials, zirconium},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955638770\&partnerID=40\&md5=31e0130d40aa381f08a781115b43fe2e},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Magallanes-Perdomo, M. and Fernandes, M.H. and Hansford, D.J. and De Aza, A.H. and Pena, P. and Monteiro, F.J.}
}
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	volume = {12},
	year = {2011},
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},
	month = {2011///},
	pages = {4183 - 4195},
	abstract = {Chitosan (Ch) is a nontoxic and biocompatible polysaccharide extensively used in biomedical applications. Ch, as a polycation, can be combined with anionic polymers by layer-bylayer (LbL) self-assembly, giving rise to multilayered complexed architectures. These structures can be used in tissue engineering strategies, as drug delivery systems, or artificial matrices mimicking the extracellular microenvironment. In this work, Ch was combined with poly(γ-glutamic acid) (γ-PGA). γ-PGA is a polyanion, which was microbially produced, and is known for its low immunogenic reaction and low cytotoxicity. Multilayered ultrathin films were assembled by LbL, with a maximum of six layers. The interaction between both polymers was analyzed by: ellipsometry, quartz crystal microbalance with dissipation, Fourier transform infrared spectroscopy, atomic force microscopy, and zeta potential measurements. Ch/γ-PGA polyelectrolyte multilayers (PEMs) revealed no cytotoxicity according to ISO 10993-5. Overall, this study demonstrates that Ch can interact electrostatically with γ-PGA forming multilayered films. Furthermore, this study provides a comprehensive characterization of Ch/γ-PGA PEM structures, elucidating the contribution of each layer for the nanostructured films. These model surfaces can be useful substrates to study cell-biomaterial interactions in tissue regeneration. {\textcopyright} 2011 American Chemical Society.},
	keywords = {Amino acids, Anionic polymers, article, Atomic force microscopy, Atomic spectroscopy, Biocompatible Materials, Biocompatible polysaccharides, Biomaterials, Biomedical applications, Cell-biomaterial interactions, chemical interaction, chitosan, controlled study, Cytotoxicity, Drug delivery, drug delivery system, Drug Delivery Systems, Electrolytes, ellipsometry, enzyme activity, Extracellular, Fourier transform infrared spectroscopy, Glutamic acid, immunogenicity, infrared spectroscopy, Layer by layer self assembly, Medical applications, microenvironment, Microenvironments, Microscopy, Atomic Force, Model surface, Models, Molecular, Multi-layered, Multi-layered films, Multilayers, nanofilm, Nanostructured Films, polyanion, Polyanions, Polycations, polyelectrolyte, Polyelectrolyte complexes, Polyelectrolyte multilayer, Polyelectrolytes, Polyethylenes, polyglutamic acid, Polymers, priority journal, Programmed control systems, Quartz, quartz crystal microbalance, Quartz crystal microbalance with dissipation, Self assembly, Tissue, Tissue engineering, Tissue regeneration, Ultrathin films, Viscoelastic Substances, Zeta potential, Zeta potential measurements},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84856258191\&partnerID=40\&md5=8ba447b7a45e8004e5b3aa3d5cff2404},
	author = {Antunes, J.C. and Pereira, C.L. and Molinos, M. and Ferreira-da-Silva, F. and Dessi, M. and Gloria, A. and Ambrosio, L. and Gonca̧lves, R.M. and Barbosa, M.A.}
}
@article {2815,
	title = {MIQuant - semi-automation of infarct size assessment in models of cardiac ischemic injury},
	journal = {PLoS ONE},
	volume = {6},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: ScopusArt. No.: e25045},
	month = {2011///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80053583980\&partnerID=40\&md5=757f7f25dcffa46f5fd66ccee74f13f0},
	author = {Nascimento, D.S. and Valente, M. and Esteves, T. and De F{\'a}tima De Pina, M. and Guedes, J.G. and Freire, A. and Quelhas, P. and Pinto-Do-{\'O}, P.}
}
@article {2231,
	title = {Molecular recognition force spectroscopy: A new tool to tailor targeted nanoparticles},
	journal = {SmallSmall},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1002/smll.201002074Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis̃o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Verma, I.M., Weitzman, M.D., (2005) Annu. Rev. Biochem., 74, pp. 711-738;Midoux, P., Breuzard, G., Gomez, J.P., Pichon, C., (2008) Curr. Gene Ther., 8, pp. 335-352; 
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},
	month = {2011///},
	pages = {1236 - 1241},
	abstract = {The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine. A novel screening tool is described to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. The unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. {\textcopyright} 2011 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {article, Atomic force microscopy, Atomic spectroscopy, atomic-force microscopy, chemistry, Event probability, Force spectroscopy, Functionalized nanoparticles, gene technology, Gene-delivery systems, Genes, Medical nanotechnology, medicine, methodology, Microscopy, Atomic Force, Molecular biology, Molecular recognition, molecular recognition force spectroscopy, Nanomedicines, nanoparticle, Nanoparticle formulation, nanoparticles, Nanotechnology, Neuronal cell, Optimization, Receptor interaction, Screening tool, Specific interaction, Targeted nanoparticle},
	isbn = {16136810 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955561627\&partnerID=40\&md5=85fa523edfcd7a6e171a53820be7f230},
	author = {Oliveira, H. and Rangl, M. and Ebner, A. and Mayer, B. and Hinterdorfer, P. and P{\^e}go, A.P.}
}
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Noel, D., Gazit, D., Bouquet, C., Short-term BMP-2 expression issufficient for in vivo osteochondral differentiation of mesenchymalstem cells (2004) Stem Cells, 22, pp. 74-85
},
	month = {2011///},
	pages = {46 - 57},
	abstract = {Mesenchymal stem cells (MSCs) can be isolated from several tissues in the body, have the ability to selfrenewal, show immune suppressive properties and are multipotent, being able to generate various cell types. At present, due to their intrinsic characteristics, MSCs are considered very promising in the area of tissue engineering and regenerative medicine. In this context, genetic modification can be a powerful tool to control the behavior and fate of these cells and be used in the design of new cellular therapies. Viral systems are very effective in the introduction of exogenous genes inside MSCs. However, the risks associated with their use are leading to an increasing search for non-viral approaches to attain the same purpose, even if MSCs have been shown to be more difficult to transfect in this way. In the past few years, progress was made in the development of chemical and physical methods for non-viral gene delivery. Herein, an overview of the application of those methods specifically to MSCs is given and their use in tissue engineering and regenerative medicine therapeutic strategies highlighted using the example of bone tissue. Key issues and future directions in non-viral gene delivery to MSCs are also critically addressed. {\textcopyright} 2011 Bentham Science Publishers Ltd.},
	keywords = {Animals, article, artificial chromosome, Bone and Bones, Bone Regeneration, bone tissue, Bone tissue engineering, cell based gene therapy, dendrimer, ex vivo gene transfer, Gene delivery, gene targeting, Gene therapy, Gene Transfer Techniques, gene vector, genetic transfection, human, Humans, in vivo gene transfer, liposome, Mesenchymal stem cell, Mesenchymal Stem Cells, nanomedicine, nanoparticle, Non-viral vectors, nonhuman, nonviral gene delivery system, polymer, protein expression, Regeneration, Regenerative Medicine, Tissue engineering, Tissue regeneration},
	isbn = {15665232 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79951686783\&partnerID=40\&md5=c9cc095fac56b04808553b19fd1f5f48},
	author = {Santos, J.L. and Pandita, D. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2216,
	title = {Novel TiN                    x-based biosignal electrodes for electroencephalography
},
	journal = {Measurement Science and TechnologyMeas. Sci. Technol.},
	volume = {22},
	year = {2011},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusArt. No.: 124007CODEN: MSTCEdoi: 10.1088/0957-0233/22/12/124007Language of Original Document: EnglishCorrespondence Address: Fiedler, P.; Institute of Biomedical Engineering and Informatics, Ilmenau University of Technology, Gustav-Kirchhoff-Str. 2, 98693 Ilmenau, Germany; email: patrique.fiedler@tu-ilmenau.deReferences: Teplan, M., Fundamentals of EEG measurement (2002) Meas. Sci. Rev., 2, p. 2. , 1-11;Fonseca, C., Silva Cunha, J.P., Martins, R.E., Ferreira, V.M., Marques De Sa, J.P., Barbosa, M.A., Martins Da Silva, A., A novel dry active electrode for EEG recording (2007) IEEE Transactions on Biomedical Engineering, 54 (1), pp. 162-165. , DOI 10.1109/TBME.2006.884649, 23; 
Cunha, L.T., Pedrosa, P., Tavares, C.J., Alves, E., Vaz, F., Fonseca, C., The role of composition, morphology and crystalline structure in the electrochemical behaviour of TiN x thin films for dry electrode sensor materials (2009) Electrochim. Acta, 55 (1), pp. 59-67; 
Dias, N.S., Carmo, A., Ferreira Da Silva, A., Mendes, P.M., Correia, J.H., New dry electrodes based on iridium oxide (IrO) for non-invasive biopotential recordings and stimulation (2010) Sensors Actuators, 164 (1-2), pp. 28-34; 
Green, R.A., Baek, S., Poole-Warren, L.A., Martens, P.J., Conducting polymer-hydrogels for medical electrode applications (2010) Sci. Technol. Adv. Mater., 11 (1), p. 014107; 
Harland, C.J., Clark, T.D., Prance, R.J., Remote detection of human electroencephalograms using ultrahigh input impedance electric potential sensors (2002) Appl. Phys. Lett., 81 (17), pp. 3284-3286; 
Von, E.N., Spinelli, E., Muravchik, C.H., Capacitive electrodes in electroencephalography (2006) Conf. Proc. IEEE Eng. Med. Biol. Soc., 1, pp. 1126-1129; 
Kingsley, S.A., Sriram, S., Pollick, A., Marsh, J., Photrode optical sensor for electrophysiological monitoring (2003) Aviat. Space Environ. Med., 74, pp. 1215-1216; 
Fernandes, M.S., Dias, N.S., Silva, A.F., Nunes, J.S., Lanceros-Mendez, S., Correia, J.H., Mendes, P.M., Hydrogel-based photonic sensor for a biopotential wearable recording system (2010) Biosens. Bioelectron., 26 (1), pp. 80-86; 
Searle, A., Kirkup, L., A direct comparison of wet, dry and insulating bioelectric recording electrodes (2000) Physiological Measurement, 21 (2), pp. 271-283. , DOI 10.1088/0967-3334/21/2/307, PII S0967333400090511; 
Merletti, R., The electrode-skin interface and optimal detection of bioelectric signals (2010) Physiol. Meas., 31; 
Piscanec, S., Ciacchi, L.C., Vesselli, E., Comelli, G., Sbaizero, O., Meriani, S., De Vita, A., Bioactivity of TiN-coated titanium implants (2004) Acta Mater., 52 (5), pp. 1237-1245; 
Vaz, F., Ferreira, J., Ribeiro, E., Rebouta, L., Lanceros-Mendez, S., Mendes, J.A., Alves, E., De Rijk, J., Influence of nitrogen content on the structural, mechanical and electrical properties of TiN thin films (2005) Surface and Coatings Technology, 191 (2-3), pp. 317-323. , DOI 10.1016/j.surfcoat.2004.01.033, PII S025789720400060X; 
Vaz, F., Machado, P., Rebouta, L., Cerqueira, P., Goudeau, Ph., Riviere, J.P., Alves, E., De Rijk, J., Mechanical characterization of reactively magnetron-sputtered TiN films (2003) Surface and Coatings Technology, 174-175, pp. 375-382. , DOI 10.1016/S0257-8972(03)00711-4; 
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Mayorga Martinez, C.C., Madrid, R.E., Felice, C.J., Electrochemical and geometrical characterization of iridium oxide electrodes in stainless steel substrate (2008) Sensors Actuators, 133 (2), pp. 682-686; 
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Graichen, U., Witte, H., Haueisen, J., Analysis of induced components in electroencephalograms using a multiple correlation method (2009) Biomed. Eng. Online, 8
},
	month = {2011///},
	abstract = {A composition, structural, morphological and electrochemical study of two titanium nitride (TiN                        x) thin films, sample A and sample B, obtained by dc reactive sputtering on titanium substrates was carried out in this paper. In order to assess their applicability to be used as dry electrodes, several EEG signal acquisition tests were performed using the TiN
                        x electrodes and compared to signals acquired with conventional silver/silver-chloride (Ag/AgCl) electrodes. The two films displayed some compositional differences, as sample B was over-stoichiometric (x = 1.34, x = N/Ti atomic ratio) and sample A was close-stoichiometric (x = 0.94). XRD diffractograms showed that both samples developed a similar fcc crystalline structure (δ-TiN phase). XRD peak fitting showed that sample B (over-stoichiometric) has a more oriented structure (highly [1 1 1] textured), with larger grains. A columnar-type structure with pyramid-like shape at column{\textquoteright}s top was common to both TiN 
                        x films. This morphology, in addition to some columnar disaggregation, gives rise to a rather rough surface and porous structure. Electrochemical impedance spectroscopy results in artificial sweat showed that the electric properties of the samples remain unchanged, even after prolonged contact with sweat. The comparison of EEG signals, simultaneously recorded using the novel TiN
                        x electrodes and conventional Ag/AgCl electrodes, showed very similar results. The root mean square deviation, which was computed for different electrode-electrolyte combinations and signal sequences of 5 s, was in the range of 4.5-9.4 νV and 6.7-20.4 νV for samples A and B, respectively. An additional test with two sets of Ag/AgCl electrodes revealed similar results, thus indicating most of the signal differences are related to the spatial distance of the compared electrodes as well as environmental noise. Therefore, our results lead to the conclusion that the novel TiN
                        x electrodes show promising signal quality for application in EEG biosignal measurements. {\textcopyright} 2011 IOP Publishing Ltd.
},
	keywords = {bioelectric, biomedical electrodes, Chlorine compounds, dc magnetron sputtering, Electric properties, Electrochemical impedance spectroscopy, Electrochemical properties, Electrodes, electroencephalography, Electrophysiology, Signal processing, signals, Surface structure, Thin films, Tinning, Titanium, Titanium nitride, titanium nitride thin films},
	isbn = {09570233 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-81555220994\&partnerID=40\&md5=477ac3097f66e1610ed5147be18c5c77},
	author = {Fiedler, P. and Cunha, L.T. and Pedrosa, P. and Brodkorb, S. and Fonseca, C. and Vaz, F. and Haueisen, J.}
}
@article {2237,
	title = {Pectin-based injectable biomaterials for bone tissue engineering},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {12},
	year = {2011},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm101110xPubMed ID: 21302960Language of Original Document: EnglishCorrespondence Address: Munarin, F.; Biomatlab, Bioengineering Department, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milan, Italy; email: fabiola.munarin@mail.polimi.itChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; osteocalcin, 136461-80-8; pectin, 9000-69-5; Biocompatible Materials; Oligopeptides; Pectins; arginyl-glycyl-aspartic acid, 99896-85-2; pectin, 9000-69-5References: Brown, M.J., Lester, J.N., (1980) Appl. Environ. Microbiol., 40, pp. 179-185;Knox, J.P., (2008) Curr. Opin. Plant Biol., 1, pp. 308-313; 
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},
	month = {2011///},
	pages = {568 - 577},
	abstract = {A variety of natural polymers and proteins are considered to be 3D cell culture structures able to mimic the extracellular matrix (ECM) to promote bone tissue regeneration. Pectin, a natural polysaccharide extracted from the plant cell walls and having a chemical structure similar to alginate, provides interesting properties as artificial ECM. In this work, for the first time, pectin, modified with an RGD-containing oligopeptide or not, is used as an ECM alternative to immobilize cells for bone tissue regeneration. The viability, metabolic activity, morphology, and osteogenic differentiation of immobilized MC3T3-E1 preosteoblats demonstrate the potential of this polysaccharide to keep immobilized cells viable and differentiating. Preosteoblasts immobilized in both types of pectin microspheres maintained a constant viability up to 29 days and were able to differentiate. The grafting of the RGD peptide on pectin backbone induced improved cell adhesion and proliferation within the microspheres. Furthermore, not only did cells grow inside but also theywere able to spread out from themicrospheres and to organize themselves in 3D structures producing a mineralized extracellular matrix. These promising results suggest that pectin can be proposed as an injectable cell vehicle for bone tissue regeneration. {\textcopyright} 2011 American Chemical Society.},
	keywords = {3-D Cell culture, 3D Structure, 3T3 Cells, Adhesion, Alginate, animal cell, Animals, arginylglycylaspartic acid, article, Biocompatible Materials, Biopolymers, Bone, Bone and Bones, Bone Regeneration, bone tissue, Bone tissue engineering, Bone tissue regeneration, carbohydrate analysis, cell adhesion, Cell culture, cell differentiation, cell growth, cell metabolism, cell proliferation, cell spreading, cell structure, cell viability, Cells, Chemical modification, Chemical structure, controlled study, Cryoelectron Microscopy, Extracellular matrices, extracellular matrix, gene expression, immobilized cell, Immobilized cells, Injectable biomaterials, Injections, MC3T3-E1, Metabolic activity, Mice, Microscopy, Electron, Scanning, microsphere, Microspheres, Mineralized extracellular matrixes, mouse, Natural polysaccharide, nonhuman, Oligopeptides, osteoblast, osteocalcin, Osteogenic differentiation, pectin, Pectins, Plant cell culture, Plant cell wall, Preosteoblasts, priority journal, RGD peptide, Three dimensional, Tissue engineering},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955409005\&partnerID=40\&md5=277a7d5e91116f9a03f144aabda0dd96},
	author = {Munarin, F. and Guerreiro, S.G. and Grellier, M.A. and Tanzi, M.C. and Barbosa, M.A. and Petrini, P. and Granja, P.L.}
}
@article {2227,
	title = {Scientists perception of ethical issues in nanomedicine: A case study},
	journal = {NanomedicineNanomedicine},
	volume = {6},
	year = {2011},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.2217/nnm.11.9PubMed ID: 21506689Language of Original Document: EnglishCorrespondence Address: Silva Costa, H.; IBMC - Instituto de Biologia Molecular e Celular, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: helena.costa@kit.eduReferences: (2006) Nanotechnology for Health, , European Commission European Technology Platform on Nanomedicine Strategic Research Agenda, Brussels, Belgium;(2005) Nanomedicine: An ESF - European Medical Research Councils (EMRC) Forward Look Report, , European Science Foundation ISBN 2-912049-52-0. IREG Strasbourg France; 
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},
	month = {2011///},
	pages = {681 - 691},
	abstract = {Aim: Research and development in nanomedicine has been accompanied by the consideration of ethical issues; however, little is known about how researchers working in this area perceive such issues. This case-study explores scientists attitude towards and knowledge of ethical issues. Method: Data were collected by semi-structured interviews with 22 nanomedicine practitioners and subject to content analysis. Results: We found that scientists reflect with ambiguity on the reputed novelty of nanomedicine and what the ethical issues and risks are in their work. Respondents see no necessity for a paradigm shift in ethical considerations, but view ethical issues in nanomedicine as overlapping with those of other areas of biomedical research. Most respondents discuss ethical issues they faced in scientific work with their colleagues, but expect benefit from additional information and training on ethics. Conclusion: Our findings that scientists are motivated to reflect on ethical issues in their work, can contribute to the design of new strategies, including training programs, to engage scientists in ethical discussion and stimulate their responsibility as nanomedicine practitioners. {\textcopyright} 2011 Future Medicine Ltd.},
	keywords = {adult, ambiguity, article, attitude, case study, clinical article, empirical research, ethics, Female, human, Humans, information, knowledge, male, medical ethics, medical research, Middle Aged, Models, Theoretical, nanomedicine, Nanotechnology, perception, priority journal, professional practice, qualitative study, Research Personnel, responsibility, risks, scientist, scientists perception, semi structured interview, training, Young Adult},
	isbn = {17435889 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959957262\&partnerID=40\&md5=33a048f12514a5e04810a0c7d7e311dd},
	author = {Silva Costa, H. and Sethe, S. and P{\^e}go, A.P. and Olsson, I.A.S.}
}
@article {2816,
	title = {Systemic delivery of bone marrow-derived mesenchymal stromal cells diminishes neuropathology in a mouse model of Krabbe{\textquoteright}s disease},
	journal = {Stem Cells},
	volume = {29},
	year = {2011},
	note = {Cited By (since 1996):4Export Date: 2 October 2013Source: Scopus},
	month = {2011///},
	pages = {1738 - 1751},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80054957115\&partnerID=40\&md5=460f6b3c9735980171039cf772450e6f},
	author = {Miranda, C.O. and Teixeira, C.A. and Liz, M.A. and Sousa, V.F. and Franquinho, F. and Forte, G. and Di Nardo, P. and Pinto-Do-{\'O}, P. and Sousa, M.M.}
}
@article {2244,
	title = {Chitosan-based gene delivery vectors targeted to the peripheral nervous system},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32874PubMed ID: 20734332Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; chitosan, 9012-76-4; imidazole, 1467-16-9, 288-32-4; macrogol, 25322-68-3; Cations; Chitosan, 9012-76-4; DNA, 9007-49-2; Polyethylene GlycolsTradenames: Chimarin, medicarb, SwedenManufacturers: medicarb, SwedenReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov Today, 1, pp. 177-185;Apfel, S.C., Neurotrophic factors in peripheral neuropathies: Therapeutic implications (1999) Brain Pathol, 9, pp. 393-413; 
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},
	month = {2010///},
	pages = {801 - 810},
	abstract = {A non-toxic, targeted, simple and efficient system that can specifically transfect peripheral sensorial neurons can pave the way towards the development of new therapeutics for the treatment of peripheral neuropathies. In this study chitosan (CH), a biodegradable polymer, was used as the starting material in the design of a multicomponent vector targeted to the peripheral nervous system (PNS). Polycation-DNA complexes were optimized using imidazole-and thiol-grafted CH (CHimiSH), in order to increase transfection efficiency and allow the formation of ligand conjugated nanocomplexes, respectively. The 50 kDa non-toxic fragment from the tetanus toxin (HC), shown to interact specifically with peripheral neurons and undergo retrograde transport, was grafted to the binary complex via a bi-functional poly(ethylene glycol) (HC-PEG) reactive for the thiol moieties present in the complex surface. The targeting of the developed nanocomplexes was assessed by means of internalization and transfection studies in the ND7/23 (neuronal) vs. NIH 3T3 (fibroblast) cell lines. Targeted transfection was further confirmed in dorsal root ganglion dissociated primary cultures. A versatile, multi-component nanoparticle system that successfully targets and transfects neuronal cell lines, as well as dorsal root ganglia (DRG) primary neuron cultures was obtained for the 1.0 (w/w) HC-PEG/DNA formulation. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {animal cell, Animals, article, Binary complexes, Biodegradable polymers, Carbohydrate Conformation, Carbohydrate Sequence, Cations, Cell culture, Cell lines, cell population, Cells, Cultured, Chitin, chitosan, Complex surface, DNA, DNA complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Ethylene Glycol, Female, Ganglia, Spinal, Gene delivery, gene delivery system, Gene delivery vectors, Gene therapy, Gene transfer, Gene Transfer Techniques, genetic transfection, Genetic Vectors, Grafting (chemical), Humans, imidazole, macrogol, materials testing, Mice, Molecular Sequence Data, Molecular Structure, mouse, Multicomponents, Nanocomplexes, Nanoparticle systems, nanoparticles, nerve cell culture, Neuron culture, Neuronal cell, NIH 3T3 Cells, nonhuman, Nontoxic fragment of tetanus toxin, Peripheral nervous system, Peripheral neuropathy, Polycations, Polyethylene Glycols, Polyethylene oxides, Pregnancy, Primary culture, rat, Rats, Rats, Wistar, spinal ganglion, Starting materials, Targeted, Tetanus toxin, Toxic materials, Transfection, Transfection efficiency},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78249240032\&partnerID=40\&md5=af9c3ca5ca712d53abb5e80f45ed09c0},
	author = {Oliveira, H. and Pires, L.R. and Fernandez, R. and Martins, M.C.L. and Sim{\~o}es, S. and P{\^e}go, A.P.}
}
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},
	month = {2010///},
	pages = {3933 - 3939},
	abstract = {Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75\% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal, Animals, article, Cell Line, cell viability, chemistry, Chitin, chitosan, cytology, Drug delivery, genetic transfection, genetics, Local delivery, metabolism, Microstructured, nanobiofunctionalized implants, nanoparticle, nanoparticles, nerve cell, nerve regeneration, Nerve repair, neurite, Neurite outgrowth, Neurites, Neurons, Polymer filaments, Prostheses and Implants, prostheses and orthoses, rhoA GTP-Binding Protein, RhoA guanine nucleotide binding protein, RNA, RNA, Small Interfering, siRNA, small interfering RNA, Spinal cords, Transfection, ultrastructure},
	isbn = {15306984 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77958033530\&partnerID=40\&md5=6862e82e01c3aedf5423ebb14248c6ec},
	author = {Mittnacht, U. and Hartmann, H. and Hein, S. and Oliveira, H. and Dong, M. and P{\^e}go, A.P. and Kjems, J. and Howard, K.A. and Schlosshauer, B.}
}
@article {2243,
	title = {Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping},
	journal = {Microscopy and MicroanalysisMicrosc. Microanal.},
	volume = {16},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: MIMIFdoi: 10.1017/S1431927610094158PubMed ID: 20964878Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Faculdade de Engenharia, Departamento de Engenharia Metalrgica e Materiais, Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; Silicon Dioxide, 7631-86-9References: Albrektsson, T., Sennerby, L., Wennerberg, A., State of the art of oral implants (2000) Periodontology, 47, pp. 15-26;Albrektsson, T., Wennerberg, A., Oral implant surfaces: Part 1-Review focusing on topographic and chemical properties of different surfaces and in vivo responses to them (2004) Int J Prosthodont, 17 (5), pp. 536-543; 
Batista, L.H., Junior, J.G., Silva, M.F., Tonholo, J., Atomic force microscopy of removal of dentin smear layers (2007) Microsc Microanal, 13 (4), pp. 245-250; 
Baudino, T.A., McFadden, A., Fix, C., Hastings, J., Price, R., Borg, T.K., Cell patterning: Interaction of cardiac myocytes and fibroblasts in three-dimensional culture (2008) MicroscMicroanal, 14 (2), pp. 117-125; 
Bohil, A.B., Robertson, B.W., Cheney, R.E., Myosin-X is a molecular motor that functions in filopodia formation (2006) Proc Natl Acad Sci USA, 103 (33), pp. 12411-12416; 
Brunette, D.M., Kenner, G.S., Gould, T.R., Grooved titanium surfaces orient growth and migration of cells from human gingival explants (1983) J Dent Res, 62 (10), pp. 1045-1048; 
Curtis, A., Wilkinson, C., Topographical control of cells (1997) Biomaterials, 18 (24), pp. 1573-1583; 
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Dalby, M.J., Riehle, M.O., Johnstone, H., Affrossman, S., Curtis, A.S., Investigating the limits of filopodial sensing: A brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia (2004) Cell Biol Int, 28 (3), pp. 229-236; 
Dur{\'a}n, A., Conde, A., Coedo, A.G., Dorado, T., Garc{\'\i}a, C., Cer{\'e}, S., Sol-gel coatings for protection and bioactiva- tion of metals used in orthopaedic devices (2004) J Mater Chem, 14 (14), pp. 2282-2290; 
Falconnet, D., Csucs, G., Grandin, H.M., Textor, M., Surface engineering approaches to micropattern surfaces for cell-based assays (2006) Biomaterials, 27 (16), pp. 3044-3063; 
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Gallego, D., Ferrell, N., Sun, Y., Hansford, D., Multilayer micromolding of degradable polymer tissue engineering scaffolds (2008) Mater Sci Eng C, 28 (3), pp. 353-358; 
Gallego-Perez, D., Higuita-Castro, N., Sharma, S., Reen, R.K., Palmer, A.F., Gooch, K.J., Lee, L.J., Hansford, D.J., High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform (2010) Lab Chip, 10 (6), pp. 775-782; 
Garcia, C., Cer{\'e}, S., Dur{\'a}n, A., Bioactive coatings prepared by sol-gel on stainless steel 316L (2004) J Non-Cryst Solids, 348, pp. 218-224; 
Healy, K.E., Thomas, C.H., Rezania, A., Kim, J.E., McKeown, P.J., Lom, B., Hockberger, P.E., Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry (1996) Biomaterials, 17 (2), pp. 195-208; 
Ito, Y., Surface micropatterning to regulate cell functions (1999) Biomaterials, 20 (23-24), pp. 2333-2342; 
Kim, E.J., Boehm, C.A., Mata, A., Fleischman, A.J., Muschler, G.F., Roy, S., Post microtextures accelerate cell proliferation and osteogenesis (2010) Acta Biomater, 6 (1), pp. 160-169; 
Lee, E.-J., Lee, S.H., Kim, H.W., Kong, Y.M., Kim, H.-E., Fluoridated apatite coatings on titanium obtained by electronbeam deposition (2005) Biomaterials, 26 (18), pp. 3843-3851; 
Lim, J.Y., Topographic control of cell response to synthetic materials (2009) Tissue Eng Regen Med, 6 (1), pp. 365-370; 
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Mata, A., Boehm, C., Fleischman, A.J., Muschler, G., Roy, S., Growth of connective tissue progenitor cells on microtextured polydimethylsiloxane surfaces (2002) J Biomed Mater Res, 62 (4), pp. 499-506; 
Mata, A., Kim, E.J., Boehm, C.A., Fleischman, A.J., Muschler, G.F., Roy, S., A three-dimensional scaffold with precise micro-architecture and surface micro-textures (2009) Biomaterials, 30 (27), pp. 4610-4617; 
Meirelles, L., Currie, F., Jacobsson, M., Albrektsson, T., Wennerberg, A., The effect of chemical and nanotopographical modifications on the early stages of osseointegration (2008) Int J Oral Maxillofac Implants, 23 (4), pp. 641-647; 
Nayab, S.N., Jones, F.H., Olsen, I., Effects of calcium ion implantation on human bone cell interaction with titanium (2005) Biomaterials, 26 (23), pp. 4717-4727; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Cells spreading on micro-fabricated silica thin film coatings (2009) Microsc Microanal, 15 (S3), pp. 77-78; 
Perrie, W., Rushton, A., Gill, M., Fox, P., O{\textquoteright}Neill, W., Femtosecond laser micro-structuring of alumina ceramic (2005) Appl Surf Sci, 248 (1-4), pp. 213-217; 
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Rompen, E., Domken, O., Degidi, M., Pontes, A.E., Piattelli, A., The effect of material characteristics, of surface topography and of implant components and connections on soft tissue integration: A literature review (2006) Clin Oral Implants Res, 17 (S2), pp. 55-67; 
Sennerby, L., Dental implants: Matters of course and controversies (2000) Periodontol, 47, pp. 9-14; 
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Teixeira, S., Monteiro, F.J., Ferraz, M.P., Vilar, R., Eugenio, S., Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies (2007) J Biomed Mater Res A, 81 (4), pp. 920-929; 
Vorobyev, A.Y., Guo, C., Femtosecond laser structuring of titanium implants (2007) Appl Surf Sci, 253 (17), pp. 7272-7280; 
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Zinger, O., Zhao, G., Schwartz, Z., Simpson, J., Wieland, M., Landolt, D., Boyan, B., Differential regulation of osteoblasts by substrate microstructural features (2005) Biomaterials, 26 (14), pp. 1837-1847
},
	month = {2010///},
	pages = {670 - 676},
	abstract = {While there has been rapid development of microfabrication techniques to produce high-resolution surface modifications on a variety of materials in the last decade, there is still a strong need to produce novel alternatives to induce guided tissue regeneration on dental implants. High-resolution microscopy provides qualitative and quantitative techniques to study cellular guidance in the first stages of cell-material interactions. The purposes of this work were (1) to produce and characterize the surface topography of isotropic and anisotropic microfabricated silica thin films obtained by sol-gel processing, and (2) to compare the in vitro biological behavior of human bone marrow stem cells on these surfaces at early stages of adhesion and propagation. The results confirmed that a microstamping technique can be used to produce isotropic and anisotropic micropatterned silica coatings. Atomic force microscopy analysis was an adequate methodology to study in the same specimen the sintering derived contraction of the microfabricated coatings, using images obtained before and after thermal cycle. Hard micropatterned coatings induced a modulation in the early and late adhesion stages of cell-material and cell-cell interactions in a geometry-dependent manner (i.e., isotropic versus anisotropic), as it was clearly determined, using scanning electron and fluorescence microscopies. {\textcopyright} Microscopy Society of America 2010.},
	keywords = {AFM, Atomic force microscopy, audiovisual equipment, cell adhesion and proliferation, Cell Culture Techniques, cell proliferation, conference paper, culture technique, cytology, Fluorescence microscopy, Guided tissue regeneration, human, Humans, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Microscopy, Fluorescence, microstamping, Motion Pictures as Topic, myeloid progenitor cell, Myeloid Progenitor Cells, Scanning electron microscopy, SEM, silica sol-gel films, silicon dioxide, Soft lithography, Surface Properties, surface property, Tissue regeneration, tissue scaffold, Tissue Scaffolds},
	isbn = {14319276 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79451471917\&partnerID=40\&md5=c68d4037bb3535a092d46c2602a8b4bd},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Ferrell, N. and Fernandes, M.H. and Hansford, D. and Monteiro, F.J.}
}
@article {2278,
	title = {Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {144},
	year = {2010},
	note = {Cited By (since 1996): 28Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2010.01.034PubMed ID: 20138937Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; CQM - Centro de Qu{\'\i}mica da Madeira, MMRG, Departamento de Qu{\'\i}mica, Universidade da Madeira, Campus Universit{\'a}rio da Penteada, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: lipid, 66455-18-3; nuclease, 9026-81-7; Cations; DNA, 9007-49-2; Dendrimers; Lipids; PAMAM StarburstReferences: Segura, T., Shea, L.D., Materials for non-viral gene delivery (2001) Annu. Rev. Mat. Res., 31, pp. 25-46;Mastrobattista, E., Bravo, S.A., van der Aa, M., Crommelin, D.J.A., Nonviral gene delivery systems: from simple transfection agents to artificial viruses (2005) Drug Discov. Today Technol., 2, pp. 103-109; 
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},
	month = {2010///},
	pages = {55 - 64},
	abstract = {A new family of gene delivery vectors is synthesized consisting of a medium-size generation PAMAM dendrimer (generation 5, with amine termini) core randomly linked at the periphery to hydrophobic chains that vary in length (12 to 16 carbon alkyl chains) and number (from 4.2 to 9.7 in average). The idea subjacent to the present work is to join the advantages of the cationic nature of the dendrimer with the capacity of lipids to interact with biological membranes. Unlike other amphiphilic systems designed for the same purpose, where the hydrophobic and hydrophilic moieties coexist in opposite sides, the present vectors have a hydrophilic interior and a hydrophobic corona. The vectors are characterized in respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA are analyzed concerning their size, ζ-potential, resistance to serum nucleases, capacity of being internalized by cells and transfection efficiency. These new vectors show a remarkable capacity for mediating the internalization of pDNA with minimum cytotoxicity, being this effect positively correlated with the -CH                        2- content present in the hydrophobic corona. Gene expression in MSCs, a cell type with relevancy in the regenerative medicine clinical context, is also enhanced using the new vectors but, in this case, the higher efficiency is shown by the vectors containing the smallest hydrophobic chains. {\textcopyright} 2010 Elsevier B.V.
},
	keywords = {amphophile, animal cell, animal experiment, article, Biological membranes, Biomedical applications, cation, Cations, Cell culture, cell membrane transport, cell type, Cellular uptake, controlled study, Cytotoxicity, dendrimer, Dendrimers, DNA, DNA binding, Dyes, Functional polymers, Gene delivery, gene delivery system, gene expression, Gene therapy, Gene transfer, Gene Transfer Techniques, gene vector, Genes, genetic transfection, Genetic Vectors, Humans, Hydrocarbons, hydrophilicity, hydrophobicity, internalization, lipid, Lipids, male, Mesenchymal stem cell, Mesenchymal Stem Cells, nonhuman, nuclease, Nucleic acids, plasmid DNA, Plasmids, Poly(amidoamine) dendrimers, polyamidoamine, Polyamidoamine dendrimers, priority journal, rat, Regenerative Medicine, Stem cells, Transfection, Vectors, Zeta potential},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953239685\&partnerID=40\&md5=51dd40fc4a666fd362b5eaa2447703dd},
	author = {Santos, J.L. and Oliveira, H. and Pandita, D. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2273,
	title = {In vitro study of the proliferation and growth of human bone marrow cells on apatite-wollastonite-2M glass ceramics},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {6},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.12.027Language of Original Document: EnglishCorrespondence Address: De Aza, A. H.; Instituto de Cer{\'a}mica y Vidrio, CSIC, C/Kelsen 5, 28049 Madrid, Spain; email: aaza@icv.csic.esChemicals/CAS: apatite, 64476-38-6; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium silicate, 1344-95-2, 13983-17-0; silicate, 12627-13-3; Apatites; Bone Substitutes; Calcium Compounds; Ceramics; Silicates; calcium silicate, 1344-95-2References: Hench, L.L., Wilson, J., (1993) An Introduction to Bioceramics, , London: World Scientific;Hench, L.L., Bioceramics: From concept to clinic (1991) J. Am. Ceram. Soc., 74, pp. 1487-1510; 
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},
	month = {2010///},
	pages = {2254 - 2263},
	abstract = {This study concerns the preparation and in vitro characterization of an apatite-wollastonite-2M bioactive glass ceramic which is intended to be used for the regeneration of hard tissue (i.e. in dental and craniomaxillofacial surgery). This bioglass ceramic has been obtained by appropriate thermal treatment through the devitrification (crystallization) of a glass with a stoichiometric eutectic composition within the Ca                        3(PO 
                        4)2-CaSiO
                        3 binary system. Crack-free specimens of the bioglass ceramic were immersed in human bone marrow cell cultures for 3, 7, 14 and 21 days, in order to study biocompatibility. Cell morphology, proliferation and colonization were assessed by scanning electron microscopy and confocal laser scanning microscopy. A total protein content assay was used to evaluate the viability and proliferation of cultured bone marrow cells. The results showed that the cells were able to adhere and proliferate on the designed material due to the essentiality of silicon and calcium as accessory factors for cell activity stimulation. {\textcopyright} 2009 Acta Materialia Inc. All rights reserved.
},
	keywords = {Apatite, Apatites, article, Bioceramics, Biocompatibility, bone marrow cell, bone prosthesis, Bone Substitutes, Calcium Compounds, calcium derivative, calcium phosphate, calcium silicate, cell activity, cell adhesion, Cell culture, cell differentiation, cell enlargement, cell growth, cell proliferation, cell structure, cell viability, Cells, Cultured, ceramics, chemistry, confocal laser microscopy, crystal structure, Crystallization, cytology, glass, Glass ceramics, human, human cell, Humans, in vitro study, materials testing, Mesenchymal stem cell, Mesenchymal Stem Cells, methodology, osteoblast, Osteoblasts, physiology, priority journal, protein content, Scanning electron microscopy, silicate, Silicates, Surface Properties, surface property, Tissue regeneration, Wollastonite, X ray fluorescence},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956625645\&partnerID=40\&md5=426d361faf4a2e3f988754fc5822ea56},
	author = {Magallanes-Perdomo, M. and De Aza, A.H. and Mateus, A.Y. and Teixeira, S. and Monteiro, F.J. and De Aza, S. and Pena, P.}
}
@article {2261,
	title = {Plasma surface modification of polycarbonate and poly(propylene) substrates for biomedical electrodes},
	journal = {Plasma Processes and PolymersPlasma Processes Polym.},
	volume = {7},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: Scopusdoi: 10.1002/ppap.200900176Language of Original Document: EnglishCorrespondence Address: Vaz, F.; Universidade do Minho, Centro de Fisica, Campus de Azur{\'e}m, 4800-058 Guimar{\~a}es, Portugal; email: fvaz@fisica.uminho.ptReferences: Moon, M.-W., Vaziri, A., (2009) Scripta Mater., 60, p. 44;Pandiyaraj, K., Selvarajan, V., Deshmukh, R.R., Gao, C., (2009) Appl. Surf. Sci., 255, p. 3965; 
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},
	month = {2010///},
	pages = {676 - 686},
	abstract = {The effects of surface plasma treatments on the properties of PP and PC substrates were studied. Main results showed that the 0.48 Pa Ar treatment promoted the most favorable changes: roughness increased from 15 to 17 nm for PC and from 12 to 30 nm for PP, while the CA lowered from 95{\textdegree} to 59{\textdegree} and from 87{\textdegree} to 35{\textdegree} for PC and PP, respectively. The effects of the activation treatment on adhesion were checked by depositing a thin layer of TiN on both untreated and 0.48 Pa Ar plasma treated PC samples, and the adhesion to both substrates compared using the standard X-cut tape test. The Ar activated samples displayed better adhesion of the TiN film than the untreated ones, confirming the benefits of the surface changes induced by the plasma treatments. (Figure Presented) {\textcopyright} 2010 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {Activation treatment, Adhesion, Ar plasmas, biomedical electrodes, Deposition, Electrodes, Functional polymers, Plasma surface modifications, Plasma treatment, Plasma treatments, Plasmas, Polymers, Propylene, Surface changes, Surface modification, Surface plasma treatment, Thin layers, TiN films, Titanium nitride},
	isbn = {16128850 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956235411\&partnerID=40\&md5=e0146f15b9c6dbbc01005ef48ca837e0},
	author = {Pedrosa, P. and Chapp{\'e}, J.-M. and Fonseca, C. and Machado, A.V. and N{\'o}brega, J.M. and Vaz, F.}
}
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Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials (2006) Biomaterials, 27, pp. 5399-5408; 
Unger, R.E., Wolf, M., Peters, K., Motta, A., Migliaresi, C., James Kirkpatrick, C., Growth of human cells on a non-woven silk fibroin net: A potential for use in tissue engineering (2004) Biomaterials, 25, pp. 1069-1075; 
Huang, Y., Yu, H., Xiao, C., PH-sensitive cationic guar gum/poly(acrylic acid) polyelectrolyte hydrogels: Swelling and in vitro drug release (2007) Carbohydr. Polym., 69, pp. 774-783; 
Edwards, J.V., Alvin, F.B., Sarah, B., Abul, J.U., Kelman, C.I., Robert, F.D., Steven, J.M., Inhibition of elastase by a synthetic cotton-bound serine protease inhibitor: In vitro kinetics and inhibitor release (1999) Wound Repair Regener., 7, pp. 106-118; 
Schneider, A., Wang, X.Y., Kaplan, D.L., Garlick, J.A., Egles, C., Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing (2009) Acta Biomater., 5, pp. 2570-2578; 
Gariani, T., McBride, J.D., Leatherbarrow, R.J., The role of the P2' position of Bowman-Birk proteinase inhibitor in the inhibition of trypsin: Studies on P2' variation in cyclic peptides encompassing the reactive site loop (1999) Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol., 1431, pp. 232-237; 
Zablotna, E., Kazmierczak, K., Jaskiewicz, A., Stawikowski, M., Kupryszewski, G., Rolka, K., Chemical synthesis and kinetic study of the smallest naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues (2002) Biochem. Biophys. Res. Commun., 292, pp. 855-859
},
	month = {2010///},
	pages = {2213 - 2220},
	abstract = {The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman-Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase-antielastase imbalance found on chronic wounds. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal cell, Animals, article, Atomic force microscopy, Biodegradation, Biological degradation, Biological functions, biomaterial, Bombyx, Bowman Birk inhibitor, Bowman-Birk inhibitors, cell proliferation, Chronic wounds, controlled study, Cytotoxicity test, Degradation, Elastase, elastase inhibitor, enzyme activity, Enzyme inhibition, Fibroins, Film composition, In-vitro, keratin, Keratins, mass spectrometry, materials testing, Mice, Microscopy, Atomic Force, NIH 3T3 Cells, nonhuman, pancreatic elastase, Peptide Fragments, Peptides, priority journal, protein binding, Protein matrices, Release rate, Silk, Silk fibroin, Spectrometry, Mass, Electrospray Ionization, Sus, Swine, Synthetic peptide, Trypsin Inhibitor, Bowman-Birk Soybean, Trypsin Inhibitors, wound fluid, Wound healing},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956552375\&partnerID=40\&md5=7d7dc9145b9655d9ba0461eb7a85d118},
	author = {Vasconcelos, A. and P{\^e}go, A.P. and Henriques, L. and Lamghari, M. and Cavaco-Paulo, A.}
}
@article {2272,
	title = {Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells},
	journal = {Molecular PharmaceuticsMol. Pharm.},
	volume = {7},
	year = {2010},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1021/mp9002877PubMed ID: 20230026Language of Original Document: EnglishCorrespondence Address: Balian, G.; Orthopaedic Research Laboratories, Department of Orthopaedics and Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, United States; email: lenat@uma.ptChemicals/CAS: Dendrimers; Green Fluorescent Proteins, 147336-22-9; PAMAM Starburst; Peptides; enhanced green fluorescent proteinReferences: V{\"a}{\"a}n{\"a}nen, H.K., Mesenchymal stem cells (2005) Ann. Med., 37, pp. 469-479;Caplan, A.I., Adult mesenchymal stem cells for tissue engineering versus regenerative medicine (2007) J. Cell Physiol., 213, pp. 341-347; 
Kay, M.A., Glorioso, J.C., Naldini, L., Viral vectors for gene therapy: The art of turning infectious agents into vehicles of therapeutics (2001) Nat. Med., 7, pp. 33-40; 
Mastrobattista, E., Bravo, S.A., Van Der Aa, M., Crommelin, D.J.A., Nonviral gene delivery systems: From simple transfection agents to artificial viruses (2005) Drug Discovery Today: Technol., 2, pp. 103-109; 
Merdan, T., Kopecek, J., Kissel, T., Prospects for cationic polymers in gene and oligonucleotide therapy against cancer (2002) Adv. Drug Delivery Rev., 54, pp. 715-758; 
Hamm, A., Krott, N., Breibach, I., Blindt, R., Bosserhoff, A.K., Efficient transfection method for primary cells (2002) Tissue Eng., 8, pp. 235-245; 
McMahon, J.M., Conroy, S., Lyons, M., Greiser, U., O{\textquoteright}Shea, C., Strappe, P., Howard, L., O{\textquoteright}Brien, T., Gene transfer into rat mesenchymal stem cells: A comparative study of viral and nonviral vectors (2006) Stem Cells Dev., 15, pp. 87-96; 
Svenson, S., Tomalia, D.A., Dendrimers in biomedical applications-reflections on the field (2005) Adv. Drug Delivery Rev., 57, pp. 2106-2129; 
Duf-s, C., Uchegbu, I.F., Schatzlein, A.G., Dendrimers in gene delivery (2005) Adv. Drug Delivery Rev., 57, pp. 2177-2202; 
Guillot, M., Eisler, S., Diederich, F., Dendritic vectors for gene transfection (2007) New J. Chem., 31, pp. 1111-1127; 
Smith, D.K., Dendrimers and the double helix-from DNA binding towards gene therapy (2008) Curr. Top. Med. Chem., 8, pp. 1187-1203; 
Kukowska-Latallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., Baker Jr., J.R., Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers (1996) Proc. Natl. Acad. Sci. U.S.A., 93, pp. 4897-4902; 
Santos, J.L., Oramas, E., P{\^e}go, A.P., Granja, P.L., Tom{\'a}s, H., Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors (2009) J. Controlled Release, 134, pp. 141-148; 
Balian, G., (2008), Bone Targeting Peptides. In United States Patent 7,323,542, issued January 29Snyder, S.L., Sobocinski, P.Z., An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines (1975) Anal. Biochem., 64, pp. 284-288; 
Haensler, J., Szoka Jr., F.C., Polyamidoamine cascade polymers mediate efficient transfection of cells in culture (1993) Bioconjugate Chem., 4, pp. 372-379; 
Hosseinkhani, H., Yamamoto, M., Inatsugu, Y., Hiraoka, Y., Inoue, S., Shimokawa, H., Tabata, Y., Enhanced ectopic bone formation using a combination of plasmid DNA impregnation into 3-D scaffold and bioreactor perfusion culture (2006) Biomaterials, 27, pp. 1387-1398; 
Perrot, S., Dutertre-Catella, H., Martin, C., Rat, P., Warnet, J.M., Resazurin metabolism assay is a new sensitive alternative test in isolated pig cornea (2003) Toxicol. Sci., 72, pp. 122-129; 
Wood, K.C., Little, S.R., Langer, R., Hammond, P.T., A family of hierarchically self-assembling linear-dendritic hybrid polymers for highly efficient targeted gene delivery (2005) Angew. Chem., Int. Ed., 44, pp. 6704-6708; 
Dan Luo, K.H., Belcheva, N., Han, E., Mark Saltzman, W., Poly(ethylene glycol)-conjugated PAMAM dendrimer for biocompatible, high-efficiency dna delivery (2002) Macromolecules, 35, pp. 3456-3462; 
Schaffer, D.V., Lauffenburger, D.A., Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery (1998) J. Biol. Chem., 273, pp. 28004-28009; 
Santos, J.L., Oliveira, H., Pandita, D., P{\^e}go, A.P., Granja, P.L., Tom{\'a}s, H., Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells (2010) J. Controlled Release, , doi: 10.1016/j.jconrel.2010.01.034
},
	month = {2010///},
	pages = {763 - 774},
	abstract = {As mesenchymal stem cells (MSCs) can differentiate into multiple cell types, the delivery of exogenous genes to this type of cell can be an important tool in tissue regeneration and engineering. However transfection of MSCs using nonviral gene delivery vectors is difficult, the development of more efficient and safe DNA vehicles being necessary. Moreover, specific transfection of MSCs may be required to avoid unwanted side effects in other tissues. In this study, a novel family of gene delivery vectors based on poly(amidoamine) (PAMAM) dendrimers functionalized with peptides displaying high affinity toward MSCs was prepared. The vectors were characterized with respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA were analyzed concerning their size, -potential, capacity of being internalized by cells and transfection efficiency. These new vectors exhibited low cytotoxicity, receptor-mediated gene delivery into MSCs and transfection efficiencies superior to those presented by native dendrimers and by partially degraded dendrimers. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal cell, animal experiment, animal tissue, Animals, article, asparaginylserylmethionylisoleucylalanylhistidylasparaginyllysylthreonylarginylmethionylhistidylglycylglycylglycylserylcysteine, Cell recognition, cell survival, Cells, Cultured, controlled study, Cytotoxicity, dendrimer, Dendrimers, DNA, DNA binding, Electrophoresis, Agar Gel, flow cytometry, Gene delivery, gene targeting, Gene Transfer Techniques, gene vector, genetic transfection, Genetic Vectors, Green Fluorescent Proteins, male, Mesenchymal stem cell, Mesenchymal Stem Cells, Models, Theoretical, multigene family, nonhuman, PAMAM dendrimers, peptide, Peptides, plasmid DNA, polyamidoamine, priority journal, rat, Rats, Rats, Wistar, serylglycylhistidylglutaminylleucylleucylleucylasparaginyllysylmethionylprolylasparaginylglycylglycylglycylserylcysteine, unclassified drug, Zeta potential},
	isbn = {15438384 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953253240\&partnerID=40\&md5=e06048334c689e2faa61e7b6b8ad9bf1},
	author = {Santos, J.L. and Pandita, D. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Balian, G. and Tom{\'a}s, H.}
}
@article {2246,
	title = {Simulation for teaching normal delivery and shoulder dystocia to midwives in training},
	journal = {Education for Health: Change in Learning and PracticeEduc. Health},
	volume = {23},
	year = {2010},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: EDHEFLanguage of Original Document: EnglishCorrespondence Address: Reynolds, A.; Simulation Centre, Department of Obstetrics and Gynaecology, Faculty of Medicine, University of PortoPortugalReferences: Wilford, A., Doyle, T.J., Integrating simulation training into the nursing curriculum (2006) British Journal of Nursing, 15 (17), pp. 926-930;Lichtman, R., Walker, D., Supporting Innovations in Midwifery Education (2003) Journal of Midwifery \& Women{\textquoteright}s Health, 48 (6), pp. 389-390; 
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Lathrop, A., Winningham, B., van de Vusse, L., Simulation-Based Learning for Midwives: Background and Pilot Implementation (2007) Journal of Midwifery \& Women{\textquoteright}s Health, 52, pp. 492-498; 
Murray, C., Grant, M.J., Howarth, M.L., Leigh, J., The use of simulation as a teaching and learning approach to support practice learning (2008) Nurse Education in Practice, 8, pp. 5-8; 
Council of Deans for Health. Simulation and Practice Learning Project: Outcome of a pilot study to the test principles for auditing simulated practice learning environments in the pre-registration nursing programme (2007) Final Report, , http://www.webcitation.org/5am8HXGA3, Nursing and Midwifery Council (NMC), Retrieved April 6, 2010 from, (Archived by WebCite{\textregistered}); 
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Morgan, R., Using clinical skills laboratories to promote theory-practice integration during first practice placement: An Irish perspective (2006) Journal of Clinical Nursing, 15, pp. 155-161; 
Issenberg, S.B., McGaghie, W.C., Hart, I.R., Mayer, J.W., Felner, J.M., Petrusa, E.R., Waugh, R.A., Ewy, G.A., Simulation Technology for Health Care Professional Skills Training and Assessment (1999) Journal of the American Medical Association, 282 (9), pp. 861-866; 
Gardner, R., Raemer, D.B., Simulation in Obstetrics and Gynecology (2008) Obstetrics and Gynecology Clinics of North America, 35, pp. 97-127; 
LeFlore, J.L., Anderson, M., Michael, J.L., Engle, W.D., Anderson, J.D., Comparison of Self-Directed Learning Versus Instructor-Modeled Learning During a Simulated Clinical Experience (2007) Simulation in Healthcare, 2, pp. 170-177; 
Seybert, A.L., Barton, C.M., Simulation-Based Learning to Teach Blood Pressure Assessment to Doctor of Pharmacy Students (2007) American Journal of Pharmaceutical Education, 71 (3), pp. 1-6; 
Hoffmann, R.L., O{\textquoteright}Donnell, J.M., Kim, Y., The Effects of Human Patient Simulators on Basic Knowledge in Critical Care Nursing with Undergraduate Senior Baccalaureate Nursing Students (2007) Simulation in Healthcare, 2, pp. 110-114; 
Shepherd, I.A., Kelly, C.M., Skene, F.M., White, K.T., Enhancing Graduate Nurses{\textquoteright} Health Assessment Knowledge and Skills Using Low-fidelity Adult Human Simulation (2007) Simulation in Healthcare, 2, pp. 16-24; 
Reynolds, A., Ayres-de-Campos, D., Bastos, L.F., van Meurs, W.L., J. B. Impact of Labor and Delivery Simulation Classes in Undergraduate Medical Learning (2008) Medical Education Online, 13, p. 14; 
Seropian, M., Dillman, D., Lasater, K., Gavilanes, J., Mannequin-Based Simulation to Reinforce Pharmacology Concepts (2007) Simulation in Healthcare, 2, pp. 218-223; 
Cioffi, J., Purcal, N., Arundell, F., A Pilot Study to Investigate the Effect of a Simulation Strategy on the Clinical Decision Making of Midwifery Students (2005) The Journal of Nursing Education, 44 (3), pp. 131-134; 
Robertson, B., An Obstetric Simulation Experience in an Undergraduate Nursing Curriculum (2006) Nurse Educator, 31 (2), pp. 74-78
},
	month = {2010///},
	abstract = {Introduction and objective: The cognitive impact of using simulation sessions in midwifery training has not been the subject of previous research. The aim of this study was to compare the effect on students{\textquoteright} knowledge of a simulation session versus an image-based lecture, for teaching routine management of normal delivery and resolution of shoulder dystocia to midwives in training. Methods: Sixty students were enrolled three weeks after a "labour and delivery" class, and randomly assigned to two groups. Ten were subsequently excluded as they were not able to attend the whole session and/or did not complete the tests. In the simulation group (n=26), students were divided into pairs and attended a 30-minute hands-on session in the simulation centre. In the lecture group (n=24), students participated in a 30-minute image-based interactive lecture. A ten-question multiple-choice test was taken before (pre-test) and after (post-test) both sessions, to evaluate students{\textquoteright} knowledge of labour and delivery and shoulder dystocia. Learner satisfaction was evaluated by adding a six question Likert scale questionnaire to the post-test. Independent t-test, paired samples t-test, and Mann-Whitney test were used for statistical analysis, setting significance at p<0.05. Results: The simulation group showed a significantly higher mean post-test score (6.38 vs. 5.16; p=0.003) and a significantly greater inter-test score progression (p<0.0001). Overall learner satisfaction was also higher in this group (p=0.0001). Conclusions: A significantly higher short-term reinforcement of knowledge and greater learner satisfaction was obtained using simulation sessions compared to image-based lectures when teaching routine management of normal delivery and resolution of shoulder dystocia to midwives in training. {\textcopyright} A Reynolds, D Ayres-de-Campos, A Pereira-Cavaleiro, L Ferreira-Bastos, 2010.},
	keywords = {Labour and delivery, Midwifery training, Patient simulators, Shoulder dystocia},
	isbn = {13576283 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78649771419\&partnerID=40\&md5=7696155b2470aa2113e2199cf3d7c28d},
	author = {Reynolds, A. and Ayres-de-Campos, D. and Pereira-Cavaleiro, A. and Ferreira-Bastos, L.}
}
@article {2275,
	title = {Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {143},
	year = {2010},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2010.01.018PubMed ID: 20093157Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; macrogol, 25322-68-3; polyethyleneimine, 74913-72-7; DNA, 9007-49-2; Imines; Peptide Fragments; Polyethylenes; Tetanus Toxin; poly(ethylene imine); tetanus toxin fragment CReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov. Today, 1 (2), pp. 177-185;Sahenk, Z., Neurotrophins and peripheral neuropathies (2006) Brain Pathol., 16 (4), pp. 311-319; 
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Boussif, O., Lezoualc{\textquoteright}h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B., A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine (1995) Proc. Natl. Acad. Sci. U.S.A., 92 (16), pp. 7297-7301; 
Coll, J.L., Chollet, P., Brambilla, E., Desplanques, D., Behr, J.P., Favrot, M., In vivo delivery to tumors of DNA complexed with linear polyethylenimine (1999) Hum. Gene Ther., 10 (10), pp. 1659-1666; 
Schaffer, D.V., Lauffenburger, D.A., Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery (1998) J. Biol. Chem., 273 (43), pp. 28004-28009; 
Zanta, M.A., Boussif, O., Adib, A., Behr, J.P., In vitro gene delivery to hepatocytes with galactosylated polyethylenimine (1997) Bioconjug. Chem., 8 (6), pp. 839-844; 
Diebold, S.S., Lehrmann, H., Kursa, M., Wagner, E., Cotten, M., Zenke, M., Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection (1999) Hum. Gene Ther., 10 (5), pp. 775-786; 
Blessing, T., Kursa, M., Holzhauser, R., Kircheis, R., Wagner, E., Different strategies for formation of PEGylated EGF-conjugated PEI/DNA complexes for targeted gene delivery (2001) Bioconjug. Chem., 12 (4), pp. 529-537; 
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Figueiredo, D.M., Hallewell, R.A., Chen, L.L., Fairweather, N.F., Dougan, G., Savitt, J.M., Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport (1997) Exp. Neurol., 145 (2 PART 1), pp. 546-554; 
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},
	month = {2010///},
	pages = {350 - 358},
	abstract = {A simple, safe and efficient system that can specifically transfect peripheral sensorial neurons can bring new answers to address peripheral neuropathies. A multi-component non-viral gene delivery vector targeted to peripheral nervous system cells was developed, using poly(ethylene imine) (PEI) as starting material. A binary DNA/polymer complex based on thiolated PEI (PEISH) was optimized, considering complex size and zeta potential and the ability to transfect a sensorial neuron cell line (ND7/23). The 50. kDa non-toxic fragment from tetanus toxin (HC), which has been previously shown to interact specifically with peripheral neurons and to undergo retrograde transport, was grafted to the complex core via a bifunctional PEG (HC-PEG) reactive for the thiol moieties present in the complex surface. Several formulations of HC-PEG ternary complexes were tested for targeting, by assessing the extent of cellular internalization and levels of transfection, in both the ND7/23 and NIH 3 T3 (fibroblast) cell lines. Targeted gene transfer to the neuronal cell line was observed for the complex formulations containing 5 and 7.5. Μg of HC-PEG. Finally, our results demonstrate that the developed ternary vectors are able to transfect primary cultures of dorsal root ganglion dissociated neurons in a targeted manner and elicit the expression of a relevant neurotrophic factor. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {Aberrations, animal cell, Animals, article, Bifunctional, Cell culture, Cell Line, Tumor, Cell lines, cell strain 3T3, Cells, Cultured, Cellular internalization, Chemical modification, Complex cores, Complex formulations, Complex surface, controlled study, DNA, DNA protein complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Female, Gene transfer, genetic transfection, Imines, internalization, macrogol, Mice, mouse, Multicomponents, nanoparticle, nanoparticles, neuroblastoma cell, Neuronal cell, Neurons, neurotrophic factor, Neurotrophic factors, NIH 3T3 Cells, Nitrogen compounds, Non-viral, Non-viral gene delivery, nonhuman, Nonviral, nonviral gene delivery system, Nucleic acids, Particle Size, Peptide Fragments, Peripheral nervous system, Peripheral neuropathy, Plasmids, Polyethylene Glycols, Polyethylene oxides, Polyethyleneimine, Polyethylenes, Primary culture, priority journal, process optimization, protein expression, rat, Rats, Rats, Wistar, Regenerative Medicine, Self-assembled, sensory nerve cell, spinal ganglion, Starting materials, Targeted, Targeted gene delivery, Ternary complex, Ternary vectors, Tetanus toxin, tetanus toxin fragment c, Transfection, unclassified drug, Zeta potential},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77952239026\&partnerID=40\&md5=077b03d49cf392defc3d0c77f2f249d1},
	author = {Oliveira, H. and Fernandez, R. and Pires, L.R. and Martins, M.C.L. and Sim{\~o}es, S. and Barbosa, M.A. and P{\^e}go, A.P.}
}
@article {2285,
	title = {TNF-α regulates the effects of irradiation in the mouse bone marrow microenvironment},
	journal = {PLoS ONEPLoS ONE},
	volume = {5},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusArt. No.: e8980doi: 10.1371/journal.pone.0008980Language of Original Document: EnglishCorrespondence Address: Cacha{\c c}o, A. S.; Angiogenesis Laboratory, Centro de Investiga{\c c}{\~a}o em Patobiologia Molecular (CIPM), Instituto Portugues de Oncologia de Francisco Gentil, Lisboa, PortugalChemicals/CAS: vasculotropin, 127464-60-2; vasculotropin A, 489395-96-2; Antibodies, Neutralizing; Matrix Metalloproteinases, 3.4.24.-; Tumor Necrosis Factor-alpha; Vascular Endothelial Growth Factor AReferences: Baud, V., Karin, M., Signal transduction by tumor necrosis factor and its relatives (2001) Trends Cell Biol, 11, pp. 372-377;Balkwill, F., Mantovani, A., Inflammation and cancer: Back to Virchow? (2001) Lancet, 357, pp. 539-345; 
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},
	month = {2010///},
	abstract = {Background: Secondary bone marrow (BM) myelodysplastic syndromes (MDS) are increasingly common, as a result of radio or chemotherapy administered to a majority of cancer patients. Patients with secondary MDS have increased BM cell apoptosis, which results in BM dysfunction (cytopenias), and an increased risk of developing fatal acute leukemias. In the present study we asked whether TNF-α, known to regulate cell apoptosis, could modulate the onset of secondary MDS. Principal Findings:We show that TNF-α is induced by irradiation and regulates BM cells apoptosis in vitro and in vivo. In contrast to irradiated wild type (WT) mice, TNF-α deficient (TNF-α KO) mice or WT mice treated with a TNF-α-neutralizing antibody were partially protected from the apoptotic effects of irradiation. Next we established a 3-cycle irradiation protocol, in which mice were sub-lethally irradiated once monthly over a 3 month period. In this model, irradiated WT mice presented loss of microsatellite markers on BM cells, low white blood cell (WBC) counts, reduced megakaryocyte (MK) and platelet levels (thrombocytopenia) and macrocytic anemia, phenoypes that suggest the irradiation protocol resulted in BM dysfunction with clinical features of MDS. In contrast, TNF-α KO mice were protected from the irradiation effects: BM cell apoptosis following irradiation was significantly reduced, concomitant with sustained BM MK numbers and absence of other cytopenias. Moreover, irradiated WT mice with long term (>=5 months) BM dysfunction had increased BM angiogenesis, MMPs and VEGF and NFkB p65, suggestive of disease progression. Conclusion:Taken together, our data shows that TNF-α induction following irradiation modulates BM cell apoptosis and is a crucial event in BM dysfunction, secondary MDS onset and progression. {\textcopyright} 2010 Cacha{\c c}o et al.},
	keywords = {angiogenesis, animal, animal cell, animal experiment, animal model, animal tissue, Animals, Antibodies, Neutralizing, apoptosis, article, Blood, blood cell count, Blotting, Western, bone marrow, bone marrow cell, Bone Marrow Cells, C57BL mouse, Cell Line, clinical feature, controlled study, cytokine production, disease course, drug effect, Enzyme Activation, flow cytometry, genetics, immunology, in vitro study, in vivo study, irradiation, leukocyte, leukocyte count, Leukocytes, macrocytic anemia, male, matrix metalloproteinase, Matrix Metalloproteinases, megakaryocyte, Megakaryocytes, metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, microenvironment, microsatellite marker, mouse, mouse mutant, Mus, myelodysplastic syndrome, neovascularization (pathology), Neovascularization, Pathologic, neutralizing antibody, nonhuman, Pathology, phenotype, protein expression, radiation dose, radiation exposure, Reverse Transcriptase Polymerase Chain Reaction, reverse transcription polymerase chain reaction, thrombocytopenia, transcription factor RelA, tumor necrosis factor alpha, tumor necrosis factor alpha antibody, Tumor Necrosis Factor-alpha, Vascular Endothelial Growth Factor A, vascularization, vasculotropin, vasculotropin A, Western blotting, zymography},
	isbn = {19326203 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77749280454\&partnerID=40\&md5=741f4070ed47c15899f89c6d21b4e8f8},
	author = {Cacha{\c c}o, A.S. and Carvalho, T. and Santos, A.C. and Igreja, C. and Fragoso, R. and Rio, C.O. and Ferreira, M. and Serpa, J. and Correia, S. and Pinto-Do-{\'O}, P. and Dias, S.}
}
@book {2319,
	title = {Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {396-398},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAEdoi: 10.4028/0-87849-353-0.303Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do PortoPortugal; email: alejopv@ineb.up.ptReferences: Warashina, H., Sakano, S., Kitamura, S., Yamauchi, K.I., (2003) Biomaterials, 24, pp. 3655-3661;Scarano, A., Piattelli, M., Caputi, S., Favero, G.A., Piattelli, A., (2004) J Periodontol, 75, pp. 292-296; 
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},
	month = {2009///},
	pages = {303 - 306},
	abstract = {Cell behaviour such as adhesion, morphology, proliferation and functional activity are highly influenced by surface properties including hydrophobicity, roughness, texture and morphology. These surface properties may be controlled using a mixture of additive coating techniques to produce glass coatings by sol-gel process and soft lithography on dental ceramics. The purpose of this work was to compare cell adhesion and early orientation of Human Bone Marrow (HBM) cells cultured on micro-patterned (micro-PGC) and on flat glass coatings (FGC) produced by sol-gel processing. Spin coating was used to apply SiO                        2 flat coatings on glass substrates as model surfaces. Photolithography was applied to produce master patterns with microscale dimensions. A moulding technique was used to print micropatterned SiO 
                        2 glass coatings produced by a sol-gel process. The coatings were then sintered, sterilized and cultured with HBM cells derived from primary cultures, using a standardized protocol, for 1 and 7 days. Cell morphology and orientation were observed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Flat and MPGC with line shaped features were produced. Cells presented a typical osteoblastic morphology on flat surfaces while slimmer, preferentially oriented and more elongated morphologies could be seen on line micro-patterned surfaces. HBM cells cultured on flat glass coatings showed increased tendency to spread and to assume more randomized proliferation when compared to the cells on the micro-patterned glass coatings. Micro-patterned glass coatings showed higher orientation control and smaller delay in the rate of proliferation, in early stages of in vitro culture as compared to flat coatings. These preliminary studies revealed that Micro-PGC induce significant morphological changes and controlled orientation of HBM cells during early stages of cell proliferation.
},
	keywords = {Adhesion, cell adhesion, Cell behaviours, Cell culture, Cell morphologies, cell proliferation, Cells, Coatings, Colloids, Confocal laser scanning microscopies, confocal microscopy, Contact guidance, Controlled orientations, cytology, Dental ceramics, Dental Materials, Flat glass, Flat surfaces, Functional activities, Gelation, Gels, glass, Glass ceramics, Glass coatings, Glass substrates, In-vitro, Micro-patterned, Micro-patterned surfaces, Micro-scale, Micromachining, Micropatterning, Model surfaces, Morphological changes, morphology, Nanostructured materials, Orientation controls, Primary cultures, Scanning, Scanning electron microscopy, Scanning electrons, SEM, Silicon compounds, Soft lithographies, Sol-gel, Sol-gel process, Sol-gel processing, Sol-gels, Sols, Surface Properties, Thermal spraying, Trace analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849141765\&partnerID=40\&md5=7a4c975e741442308a8db69108047673},
	author = {Pelaez-Vargas, A. and Ferrell, N. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
@article {2297,
	title = {Fibronectin-mediated endothelialisation of chitosan porous matrices},
	journal = {BiomaterialsBiomaterials},
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	year = {2009},
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},
	month = {2009///},
	pages = {5465 - 5475},
	abstract = {Chitosan (Ch) porous matrices were investigated regarding their ability to be colonized by human microvascular endothelial cells (HPMEC-ST1.6R cell line) and macrovascular endothelial cells namely HUVECs. Specifically we assessed if previous incubation of Ch in a fibronectin (FN) solution was effective in promoting endothelial cell (EC) adhesion to Ch matrices with different degrees of acetylation (DAs). Upon FN physiadsorption, marked differences were found between the two DAs investigated, namely DA 4\% and 15\%. While cell adhesion was impaired on Ch with DA 15\%, ECs were able to not only adhere to Ch with DA 4\%, but also to spread and colonize the scaffolds, with retention of the EC phenotype and angiogenic potential. To explain the observed differences between the two DAs, protein adsorption studies using                        125I-FN and immunofluorescent labelling of FN cell-binding domains were carried out. In agreement with the higher cell numbers found, scaffolds with DA 4\% revealed a higher number of exposed FN cell-binding domains as well as greater ability to adsorb FN and to retain and exchange adsorbed FN in the presence of competitive proteins. These findings suggest that the DA is a key parameter modulating EC adhesion to FN-coated Ch by influencing the adsorbed protein layer. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.
},
	keywords = {Acetylation, Adhesion, Adsorption, angiogenesis, article, Biocompatible Materials, Blood vessel prostheses, cell adhesion, Cell culture, cell invasion, Cell Line, Cell lines, Cell membranes, Cell number, cell proliferation, Cell size, cell spreading, cell survival, Cell-binding domain, Chitin, Chitin/chitosan, chitosan, colony formation, controlled study, Degrees of acetylation, Electric conductivity measurement, Electric currents, Endothelial cell, Endothelial Cells, Endothelialisation, endothelium, endothelium cell, epithelization, Fibronectin, Fibronectins, human, human cell, Human microvascular endothelial cells, Humans, immunofluorescence test, Key parameters, Labels, materials testing, Matrix algebra, Neovascularization, Physiologic, phenotype, porosity, Porous matrixs, priority journal, Protein adsorption, Protein layers, Scaffold, Scaffolds, Self assembly, Tissue engineering, tissue scaffold, umbilical vein},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68549107574\&partnerID=40\&md5=9babb57c1a4f2ae6eb7e7e0643bffe48},
	author = {Amaral, I.F. and Unger, R.E. and Fuchs, S. and Mendon{\c c}a, A.M. and Sousa, S.R. and Barbosa, M.A. and P{\^e}go, A.P. and Kirkpatrick, C.J.}
}
@article {2296,
	title = {Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
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},
	month = {2009///},
	pages = {2995 - 3006},
	abstract = {Chitosan was functionalized with imidazole moieties (CHimi) with the aim of improving its buffering capacity and promoting the endosomal escape ability of chitosan-DNA complexes, ultimately increasing their transfection efficiency. 5.6\%, 12.9\% and 22.1\% of the glucosamine residues of chitosan were substituted. Complexes with different molar ratios of primary amines to DNA phosphate anion (N/P) were prepared by a coacervation method. For an N/P \&gt; 3, CHimi polymers are able to complex electrostatically with DNA and condense it into positively charged nanostructures (average size 260 nm and zeta potential +16 mV at pH 5.5). In the concentration range 2.5-100 μg ml},
	keywords = {article, beta galactosidase, Biocompatible Materials, Buffers, Cell Line, cell transport, Chemical structure, chitosan, coacervation, complex formation, controlled study, Cytotoxicity, Diffusion, DNA, Drug Carriers, Drug Compounding, endosome, Gene delivery, Gene transfer, genetic transfection, human, human cell, Humans, imidazole, Kidney, materials testing, nanomaterial, nanoparticle, priority journal, protein expression, proton transport, Tissue engineering, Transfection},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70349162856\&partnerID=40\&md5=fb6672dd0cf06d6ced21eec115b597d2},
	author = {Moreira, C. and Oliveira, H. and Pires, L.R. and Sim{\~o}es, S. and Barbosa, M.A. and P{\^e}go, A.P.}
}
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Lopez, T., Ortiz-Islas, E., Manjarrez, J., Rodr{\'\i}guez-Reinoso, F., Sep{\'u}lveda, A., Gonzalez, R.D., (2006) Opt. Mater., 29, p. 70
},
	month = {2009///},
	pages = {160 - 170},
	abstract = {Titanium oxides have anti-inflammatory activity and tunable electrochemical properties that make them attractive materials for biomedical applications. This work investigated the compatibility of nanometric coatings of low-temperature phases of TiO                        2 with neurons in 4-day and 10-day cultures, using different cell densities to quantify cell survival and neurite extension. TiO
                        2 films were prepared by sol-gel and thermal treatment (250-450~{\textdegree}C) of hydrolyzed titanium tetra-isopropoxide on electrically conducting or insulating slides. The conducting substrates were not passivated by the nanometric oxide layer and could be used as electrodes. Characterization of the films showed nano-structured TiO
                        2 containing exclusively Ti
                        +4 valence in anatase and amorphous phases. When coated with polylysine, all films permitted good neuron attachment and survival for at least ten days in culture. However, they generally reduced neurite growth compared to cell culture borosilicate glass, with dendrites more affected than axons. The analyses of surface topography, hydrophilicity, charge and chemical composition revealed that TiO
                        2 chemistry was the main factor responsible for neurite inhibition. {\textcopyright} 2009 Elsevier B.V. All rights reserved.
},
	keywords = {Amorphous phasis, Anatase, Anti-inflammatory activity, Biomedical applications, Borosilicate glass, Cell culture, cell density, Cell membranes, cell survival, Chemical compositions, Coatings, Conducting substrates, Conductive films, Electrochemical properties, Gelation, Growth kinetics, Heat treatment, Iso-propoxide, Low temperature phasis, Nano-structured, Nanometrics, Nanostructured surface, Nanostructures, Neural cell, Neural cells, Neurite extension, Neurite growth, Neurites, Neurons, Oxide layer, polylysine, Surface topography, Thermal treatment, TiO, Titanium, Titanium dioxide, Titanium oxide, Titanium oxides},
	isbn = {00406090 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-69649101935\&partnerID=40\&md5=08dd6874d6b2e9bbe7310add08057bc7},
	author = {Collazos-Castro, J.E. and Cruz, A.M. and Carballo-Vila, M. and Lira-Cant{\'u}, M. and Abad, L. and del Pino, {\'A}.P. and Fraxedas, J. and San Juan, A. and Fonseca, C. and P{\^e}go, A.P. and Casa{\~n}-Pastor, N.}
}
@article {2313,
	title = {Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {134},
	year = {2009},
	note = {Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2008.11.007PubMed ID: 19070635Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; Centro de Qu{\'\i}mica da Madeira, Departamento de Qu{\'\i}mica, Universidade da Madeira, Campus Universitario da Penteada, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; calcium, 14092-94-5, 7440-70-2; osteocalcin, 136461-80-8; phosphate, 14066-19-4, 14265-44-2; BMP2 protein, human; Bone Morphogenetic Protein 2; Dendrimers; PAMAM Starburst; Polyamines; beta-Galactosidase, 3.2.1.23References: Dai, J., Rabie, A.B.M., Hagg, U., Xu, R.A., Alternative gene therapy strategies for the repair of craniofacial bone defects (2004) Curr. Gene Ther., 4 (4), pp. 469-485;Gersbach, C.A., Phillips, J.E., Garcia, A.J., Genetic engineering for skeletal regenerative medicine (2007) Annu. Rev. Biomed. Eng., 9, pp. 87-119; 
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},
	month = {2009///},
	pages = {141 - 148},
	abstract = {This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a β-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {alkaline phosphatase, amine, Amines, animal cell, Animals, article, beta galactosidase, beta-Galactosidase, Bone, Bone morphogenetic protein, bone morphogenetic protein 2, Bone Regeneration, Calcium, Cell culture, cell differentiation, Cell Line, cell lineage, cell survival, Cells, Cells, Cultured, controlled study, dendrimer, Dendrimers, embryo, enzyme activity, Flowcharting, gene expression, Gene transfer, Gene Transfer Techniques, gene vector, Genes, Reporter, genetic transfection, human, human cell, Humans, in vitro study, Kidney, male, Mesenchymal stem cell, Mesenchymal Stem Cells, nonhuman, Nonviral gene delivery, nonviral gene delivery system, Nucleic acids, osteoblast, osteocalcin, Osteogenesis, PAMAM dendrimers, phosphate, plasmid, Plasmids, polyamidoamine, Polyamines, priority journal, protein secretion, Proteins, qualitative analysis, quantitative analysis, rat, Rats, Rats, Wistar, reporter gene, staining},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-60649096454\&partnerID=40\&md5=90df49394a581738b41bf29bad341ae8},
	author = {Santos, J.L. and Oramas, E. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2308,
	title = {Physical characterization of hydroxyapatite porous scaffolds for tissue engineering},
	journal = {Materials Science and Engineering CMater. Sci. Eng. C},
	volume = {29},
	year = {2009},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.msec.2008.09.052Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: smsilva@ineb.up.ptReferences: Jones, A.C., Milthorpe, B., Averdunk, H., Limaye, A., Senden, T.J., Sakellariou, A., (2004) Biomaterials, 25 (20), p. 4947;Karageorgiou, V., Kaplan, D., (2005) Biomaterials, 26 (27), p. 5474; 
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},
	month = {2009///},
	pages = {1510 - 1514},
	abstract = {The present study refers to the preparation and characterization of porous hydroxyapatite scaffolds to be used as matrices for bone regeneration or as specific release vehicles. Ceramics are widely used for bone tissue engineering purposes and in this study, hydroxyapatite porous scaffolds were produced using the polymer replication method. Polyurethane sponges were used as templates and impregnated with a ceramic slurry at different ratios, and sintered at 1300~{\textdegree}C following a specific thermal cycle. The characteristics of the hydroxyapatite porous scaffolds and respective powder used as starting material, were investigated by using scanning electron microscopy, particle size distribution, X-ray diffraction, Fourier transformed infrared spectroscopy and compressive mechanical testing techniques. It was possible to produce highly porous hydroxyapatite scaffolds presenting micro and macropores and pore interconnectivity. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Apatite, Bone, Bone regenerations, Bone tissue engineerings, Ceramic materials, Ceramic slurries, Fourier-transformed infrared spectroscopies, hydroxyapatite, infrared spectroscopy, Macro-pores, Mechanical testing, Physical characterizations, Polymer blends, Polymer replications, Polymers, Polyurethane sponges, Pore interconnectivity, Porous hydroxyapatites, Porous scaffolds, Scaffolds, Scanning electron microscopy, Spectroscopic analysis, Starting materials, Thermal cycles, Tissue engineering, X- ray diffractions},
	isbn = {09284931 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67349181295\&partnerID=40\&md5=ea36fd01a8565ecbd7230948b317995d},
	author = {Teixeira, S. and Rodriguez, M.A. and Pena, P. and De Aza, A.H. and De Aza, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2290,
	title = {The role of composition, morphology and crystalline structure in the electrochemical behaviour of TiN                    x thin films for dry electrode sensor materials
},
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},
	month = {2009///},
	pages = {59 - 67},
	abstract = {A morphological, structural and electrochemical study of titanium nitride (TiN                        x) thin films, obtained by DC reactive sputtering on titanium substrates, was carried out for a wide range of compositions (0 \&lt; x \&lt; 1.34) aiming a selection of the best coatings for dry biomedical electrodes. The films displayed a columnar-type structure, with morphologies strongly dependent on the composition: a compact and smooth surface was found for the Ti-rich films, (x \&lt; 1), whereas the N-rich films, (x >= 1) displayed a rough and porous structure. The electrochemical study of the TiN
                        x films was performed in synthetic sweat, aiming at simulating the contact with the skin. The voltammetric analysis showed anodic currents higher for TiN
                        x films than for titanium for low and medium polarization potentials, whereas for potentials beyond 2 V the blocking behaviour of the TiN
                        x films allowed them to display lower current values. The passive dissolution currents in the sub-μA/cm
                        2 range and the charge transfer resistances of the order of the MΩ proved the excellent stability of all films in sweat conditions. Finally, the electrochemical noise analysis showed that the near-stoichiometric and N-rich films display the lowest noise, being therefore the most suitable for electrode applications, where signals in the microvolt range, such as the electroencephalographic (EEG) signals, are to be monitored. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.
},
	keywords = {Anodic currents, Bioelectric phenomena, Biomedical devices, biomedical electrodes, Blocking behaviour, Charge transfer, Charge transfer resistance, Chemical analysis, Crystalline structure, DC reactive sputtering, Dissolution, Dry electrode, EIS, Electrochemical behaviour, Electrochemical corrosion, Electrochemical electrodes, Electrochemical Noise Analysis, Electrochemical studies, Electroencephalographic signals, Ion exchange, morphology, Polarization potential, Porous structures, Selection of the best, Sensor materials, Smooth surface, Sputtering, Thin films, Titanium, Titanium nitride, Titanium substrates, Type structures, Voltammetric analysis},
	isbn = {00134686 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70349511936\&partnerID=40\&md5=171cf38af18a50e355fc5979c5fc5e7a},
	author = {Cunha, L.T. and Pedrosa, P. and Tavares, C.J. and Alves, E. and Vaz, F. and Fonseca, C.}
}
@article {2336,
	title = {Injectability of a bone filler system based on hydroxyapatite microspheres and a vehicle with in situ gel-forming ability},
	journal = {Journal of Biomedical Materials Research - Part B Applied BiomaterialsJ. Biomed. Mater. Res. Part B Appl. Biomater.},
	volume = {87},
	year = {2008},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: JBMRGdoi: 10.1002/jbm.b.31066PubMed ID: 18437700Language of Original Document: EnglishCorrespondence Address: Oliveira, S. M.; Escola Superior de Tecnologia de Viseu (ESTV), Dep. de Eng. Mec{\^a}nica e Gest{\~a}o Industrial, 3504-510 Viseu, Portugal; email: serafim@ineb.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium carbonate, 13397-26-7, 13701-58-1, 14791-73-2, 471-34-1; gluconolactonase, 9012-44-6, 9012-73-1; hydroxyapatite, 1306-06-5, 51198-94-8; Bone Substitutes; Durapatite, 1306-06-5; GelsTradenames: Captal S, Plasma BiotalManufacturers: Plasma BiotalReferences: Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373;Grabowski, G.A., Leslie, N., Wenstrup, R., Enzyme therapy for Gaucher disease: The first 5 years (1998) Blood Rev, 12, pp. 115-133; 
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Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Bioceramics, 254, pp. 877-880; 
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},
	month = {2008///},
	pages = {49 - 58},
	abstract = {The aim of this study was to test the injectability of a bone filler system based on the combination of ceramic microspheres with a gel-like vehicle, for noninvasive surgery. Porous hydroxyapatite microspheres with a uniform size and an average diameter of 535 {\textpm} 38 μm were prepared, and their compression strength and friability were tested. The sodium-alginate solution with a concentration of 7.25\% (w/v) was used as the vehicle. To promote its in situ gelation, calcium carbonate and D-gluconic-δ-lactone were added to the solution. Microspheres were mixed with the vehicle at different percentages (20-40 wt \%). Gelation times in the range of 8-20 min, were obtained, depending on the formulation. Mixtures of HAp microspheres with alginate solution at 7.25\% originating a gel in 11 min present an adequate handling time to perform an injection. Their injectability was evaluated using an injection device commonly employed in vertebroplasty surgical procedures, coupled to a texturometer in compression mode. Using an extrusion rate of 0.1 mm/s, the force required to extrude any of the mixtures tested was lower than 100 N. For an extrusion rate of 1 mm/s mixtures with 40 wt \% of microspheres were very difficult to inject. Mixtures with 35 wt \% of microspheres presented the best compromise between injectability and compression strength of the gelled system. MicroCT analysis revealed a homogeneous distribution of the microspheres inside the vehicle, as well as full interconnection of the intra-microspheres spaces. The compression strength for the gelled systems ranged from 80 kPa (gel itself) to 600 kPa (composite with 40 Wt \% of microspheres). {\textcopyright} 2008 Wiley Periodicals, Inc.},
	keywords = {Alginate, alginic acid, Apatite, article, Average diameter, bioengineering, Bone, bone filler system, Bone Substitutes, Calcium, Calcium carbonate, Ceramic, Ceramic microspheres, Coagulation, Colloids, compression, Compression mode, Compression strengths, Compressive Strength, Computerized tomography, Concentration (process), Data compression, Durapatite, Extrusion, Extrusion rates, Fillers, Forming, Gel-forming ability, Gelation, Gels, gluconolactonase, Handling time, Homogeneous distribution, hydrogel, hydroxyapatite, In situ gelation, in situ hybridization, In-situ, Injectability, injection, Injection (oil wells), Micro-CT, microsphere, Microspheres, Mixtures, non invasive procedure, Particle Size, Porous hydroxyapatite, Sodium, Surgery, Surgical procedures, Uniform size, Vehicles, Vertebroplasty},
	isbn = {15524973 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-52449123139\&partnerID=40\&md5=cc648bad01cb2c6aa01d885acac10e8a},
	author = {Oliveira, S.M. and Barrias, C.C. and Almeida, I.F. and Costa, P.C. and Pena Ferreira, M.R. and Bahia, M.F. and Barbosa, M.A.}
}
@article {2350,
	title = {Microstructure, mechanical properties and chemical degradation of brazed AISI 316 stainless steel/alumina systems},
	journal = {Materials Science and Engineering AMater. Sci. Eng. A},
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	year = {2008},
	note = {Cited By (since 1996): 11Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.msea.2007.07.015Language of Original Document: EnglishCorrespondence Address: Paiva, O.C.; Instituto Superior de Engenharia do Porto (ISEP), Rua Dr. Antonio Bernardino Almeida 431, 4200-072 Porto, Portugal; email: omp@isep.ipp.ptReferences: Schwartz, M.M., (1990) Ceramic Joining, pp. 75-140. , Schwartz M.M. (Ed), ASM International, Ohio;Nicholas, M.G., (1986) Br. Ceram. Trans. J., 85, pp. 144-146; 
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},
	month = {2008///},
	pages = {306 - 315},
	abstract = {The main aims of the present study are simultaneously to relate the brazing parameters with: (i) the correspondent interfacial microstructure, (ii) the resultant mechanical properties and (iii) the electrochemical degradation behaviour of AISI 316 stainless steel/alumina brazed joints. Filler metals on such as Ag-26.5Cu-3Ti and Ag-34.5Cu-1.5Ti were used to produce the joints. Three different brazing temperatures (850, 900 and 950 {\textdegree}C), keeping a constant holding time of 20 min, were tested. The objective was to understand the influence of the brazing temperature on the final microstructure and properties of the joints. The mechanical properties of the metal/ceramic (M/C) joints were assessed from bond strength tests carried out using a shear solicitation loading scheme. The fracture surfaces were studied both morphologically and structurally using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction analysis (XRD). The degradation behaviour of the M/C joints was assessed by means of electrochemical techniques. It was found that using a Ag-26.5Cu-3Ti brazing alloy and a brazing temperature of 850 {\textdegree}C, produces the best results in terms of bond strength, 234 {\textpm} 18 MPa. The mechanical properties obtained could be explained on the basis of the different compounds identified on the fracture surfaces by XRD. On the other hand, the use of the Ag-34.5Cu-1.5Ti brazing alloy and a brazing temperature of 850 {\textdegree}C produces the best results in terms of corrosion rates (lower corrosion current density), 0.76 {\textpm} 0.21 μA cm},
	keywords = {Active metal brazing, Alumina, Bond strength (materials), Brazing, Electrochemical degradation, Filler metals, fracture, Interface composition, Metal/ceramic joining, Metallic matrix composites, morphology, Processing parameters, Shear strength, Stainless steel},
	isbn = {09215093 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-41149104604\&partnerID=40\&md5=6dfded1210945bb66208633744939f64},
	author = {Paiva, O.C. and Barbosa, M.A.}
}
@article {2354,
	title = {PLD bioactive ceramic films: The influence of CaO-P                    2O 
                    5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells
},
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Okumura, A., Goto, M., Goto, T., Yoshinari, M., Masuko, S., Katsuki, T., Tanaka, T., (2001) Biomaterials, 22, p. 2263; 
Kim, H.J., Kim, S.H., Kim, M.S., Lee, E.J., Oh, H.G., Oh, W.M., Park, S.W., Dean, D.D., (2005) J. Biomed. Mater. Res., 74, p. 366
},
	month = {2008///},
	pages = {1775 - 1785},
	abstract = {This work consists on the evaluation of the in vitro performance of Ti6Al4V samples PLD (pulsed laser deposition) coated with hydroxyapatite, both pure and mixed with a CaO-P                        2O
                        5 glass. Previous studies on immersion of PLD coatings in SBF, showed that the immersion apatite films did not present the usual cauliflower morphology but replicated the original columnar structure and exhibited good bioactivity. However, the influence of glass associated to hydroxyapatite concerning adhesion, proliferation and morphology of MG63 cells on the films surface was unclear. In this study, the performance of these PLD coated samples was evaluated, not only following the physical-chemical transformations resulting from the SBF immersion, but also evaluating the cytocompatibility in contact with osteoblast-like MG63 cells. SEM and AFM confirmed that the bioactive ceramic PLD films reproduce the substrate{\textquoteright}s surface topography and that the films presented good adherence and uniform surface roughness. Physical-chemical phenomena occurring during immersion in SBF did not modify the original columnar structure. In contact with MG63 cells, coated samples exhibited very good acceptance and cytocompatibility when compared to control. The glass mixed with hydroxyapatite induced higher cellular proliferation. Cells grown on these samples presented many filipodia and granular structures, typical features of osteoblasts. {\textcopyright} 2007 Springer Science+Business Media, LLC.
},
	keywords = {Apatite, Apatite films, Bioactive ceramic films, Bioactive glass, Bioactivity, Bioceramics, Biocompatible Materials, Biofilms, Brassica oleracea var. botrytis, Calcium Compounds, calcium oxide, Cauliflower morphology, cell adhesion, cell proliferation, cell structure, cell survival, ceramics, Columnar structure, conference paper, controlled study, Durapatite, glass, human, human cell, Humans, hydroxyapatite, Lasers, materials testing, Microscopy, Atomic Force, Microscopy, Electron, Scanning, morphology, osteoblast, Osteoblasts, oxide, Oxides, phosphorous pentoxide, Phosphorus Compounds, Polystyrenes, priority journal, Pulsed laser deposition, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-40949140642\&partnerID=40\&md5=de09d5133a0616668cb5e57135d91ae6},
	author = {Oliveira, G.M. and Ferraz, M.P. and Gonz{\'a}lez, P.G. and Serra, J. and Leon, B. and P{\`e}rez-Amor, M. and Monteiro, F.J.}
}
@article {2351,
	title = {Surface characterization and cell response of a PLA/CaP glass biodegradable composite material},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {85},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31546PubMed ID: 17729262Language of Original Document: EnglishCorrespondence Address: Ginebra, M. P.; Department of Materials Science, Institute of Bioengineering of Catalonia (IBEC), Technical University of Catalonia, Avda Diagonal 647, 08028-Barcelona, Spain; email: maria.pau.ginebra@upc.eduChemicals/CAS: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, 298-93-1; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; osteocalcin, 136461-80-8; polylactic acid, 26100-51-6; Alkaline Phosphatase, EC 3.1.3.1; Bone Substitutes; calcium phosphate, 10103-46-5; Calcium Phosphates; Lactic Acid, 50-21-5; Osteocalcin, 104982-03-8; poly(lactic acid), 26100-51-6; PolymersReferences: Barrows, T., Degradable implants materials: A review of synthetic absorbable polymers and their applications (1986) Clin Mater, 1, pp. 233-267;Ciccone, W., Motz, C., Bentley, C., Tasto, J., Bioabsorbable implants in orthopaedics: New developments and clinical applications (2001) J Am Acad Orthop Surg, 9, pp. 280-288; 
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B{\"o}stman O Current concepts review. Absorbable implants for fracture fixation. J Bone Joint Surgery 1991;73A:148-153Ashammakhi, N., Suuronen, R., Tiainen, J., Tormala, P., Waris, T., Spotlight on naturally absorbable osteofixation devices (2003) J Craniofac Surg, 14, pp. 247-259; 
Hou, Q.P., Grijpma, D.W., Feijen, J., Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique (2003) Biomaterials, 24, pp. 1937-1947; 
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Ragel, C.V., Vallet-Regi, M., In vitro bioactivity and gentamicin release from glass-polymer-antibiotic composites (2000) J Biomed Mater Res, 51, pp. 424-429; 
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Navarro, M., Ginebra, M.P., Clement, J., Martinez, S., Avila, G., Planell, J.A., Physicochemical degradation of titania-stabilized soluble phosphate glasses for medical applications (2003) J Am Ceram Soc, 86, pp. 1345-1352; 
Navarro, M., Ginebra, M.P., Planell, J.A., Barrias, C.C., Barbosa, M., In vitro degradation behavior of a novel bioabsorbable composite material based on PLA and a soluble CaP glass (2005) Acta Biomaterialia, 1, pp. 411-419; 
Stephansson, S.N., Byers, B.A., Garcia, A.J., Enhanced expression of the osteoblastic phenotype on substrates that modulate fibronectin conformation and integrin receptor binding (2002) Biomaterials, 23, pp. 2527-2534; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res A, 66, pp. 247-259; 
Beumer, G., van Blitterswijk, C., Bakker, D., Ponec, M., Cell-seeding and in vitro biocompatibility evaluation of polymeric matrices of PEO/PBT copolymers and PLLA (1993) Biomaterials, 14, pp. 598-604; 
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Navarro, M., Ginebra, M.P., Planell, J.A., Cellular response to calcium phosphate glasses with controlled solubility (2003) J Biomed Mater Res A, 67, pp. 1009-1015; 
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},
	month = {2008///},
	pages = {477 - 486},
	abstract = {Bioabsorbable materials are of great interest for bone regeneration applications, since they are able to degrade gradually as new tissue is formed. In this work, a fully biodegradable composite material containing polylactic acid (PLA) and calcium phosphate (CaP) soluble glass particles has been characterized in terms of surface properties and cell response. Cell cultures were performed in direct contact with the materials and also with their extracts, and were evaluated using the MTT assay, alkaline phosphatase activity, and osteocalcin measurements. The CaP glass and PLA were used as reference materials. No significant differences were observed in cell proliferation with the extracts containing the degradation by-products of the three materials studied. A relation between the materials wettability and the material-cell interactions at the initial stages of contact was observed. The most hydrophilic material (CaP glass) presented the highest cell adhesion values as well as an earlier differentiation, followed by the PLA/glass material. The incorporation of glass particles into the PLA matrix increased surface roughness. SEM images showed that the heterogeneity of the composite material induced morphological changes in the cells cytoskeleton. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, Absorbable Implants, alkaline phosphatase, article, Biodegradable composite materials, biodegradable implant, Biodegradation, Bone, Bone Regeneration, Bone Substitutes, calcium phosphate, Calcium phosphates, cell adhesion, Cell culture, cell function, cell interaction, Cell Line, cell proliferation, Cell response, Composite materials, cytoskeleton, glass, human, human cell, Humans, hydrophilicity, hydrophobicity, In vitro test, Lactic Acid, materials testing, Osteoblasts, osteocalcin, polylactic acid, Polymers, scanning electron microscope, Surface analysis, Surface Properties, wettability},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-42149187233\&partnerID=40\&md5=8942b2e00b94ff0f723261e1893d04b5},
	author = {Navarro, M. and Engel, E. and Planell, J.A. and Amaral, I. and Barbosa, M. and Ginebra, M.P.}
}
@book {2385,
	title = {Characterization of hydroxyapatite sputtered films doped with titanium},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 I},
	year = {2007},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ribeiro, A.R.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: arr@ineb.up.ptReferences: Bhat, (2002) Biomaterials, , Narosa Publishing House/Kluwer Academic Publishers, India;Feddes B, Vredenberg AM. Wolke JGC, Jansen JA.: Surface and Coatings Technology 185 (2-3) (2004), p. 346Gisep, A., (2002) Injury-International Journal of the Care of the Injured, 33 (SUPPL. 2), p. 88; 
Ooms, E.M., Wolke, J.G.C., van der Waerden, J.P.C.M., (2002) Journal of Biomedical Materials Research, 61 (1), p. 9; 
Barrias, C.C., Ribeiro, C.C., Rodriguez, D., S{\'a} Miranda, C., Barbosa, M.A., (2005) Key Engineering Materials, 284-286, p. 689; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., (2004) Biomaterials, 25, p. 4363; 
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Hulshoff, J.E.G., van Dijk, K., de Ruijter, J.E., (1998) Journal of Biomedical Materials Research, 40 (3), p. 464; 
Yang, Y.Z., Kim, K.H., Ong, J.L., (2005) Biomaterials, 26 (3), p. 327; 
Ong, J.L., Lucas, L.C., (1998) Biomaterials, 19 (4-5), p. 455; 
Thian, E.S., Huang, J., Best, S.M., Barber, Z.H., Bonfield, W., (2005) Biomaterials, 26, p. 2947
},
	month = {2007///},
	pages = {649 - 652},
	abstract = {In the present work, thin films of hydroxyapatite (HAp), titanium, and hydroxyapatite doped with different titanium concentrations were obtained by sputtering and characterized using several analytical techniques. These films are intended to be used as model surfaces on protein adsorption studies in order to better understand the role of titanium ions in biological processes.},
	keywords = {Adsorption, Biofilms, Doping (additives), hydroxyapatite, Hydroxyapatite sputtered films, Model surfaces, Proteins, Sputtering, Titanium, Titanium ions},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846300257\&partnerID=40\&md5=7946215d8f37e0e216b57336b89f0f02},
	author = {Ribeiro, A.R. and Piedade, A.P. and Ribeiro, C.C. and Vieira, M.T. and Barbosa, M.A.}
}
@book {2373,
	title = {Detection of lung nodule candidates in chest radiographs},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4478 LNCS},
	year = {2007},
	note = {Conference code: 70962Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pereira, C.S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Sinal e Imagem, Campus FEUP (Faculdade de Engenharia da Universidade do Porto), Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: cmsp@fe.up.ptReferences: Wei, J., Hagihara, Y., Shimizu, A., Kobatake, H., Optimal image feature set for detecting lung nodules on chest X-ray images (2002) CARS 2002;Keserci, B., Yoshida, H., Computerized detection of pulmonary nodules in chest radiographs based on morphological features and wavelet snake model (2002) Medical Image Analysis, 6, pp. 431-447; 
Yoshida, H., Local contralateral subtraction based on bilateral symmetry of lung for reduction of false positives in computerized detection of pulmonary nodules, Biomedical Engineering (2004) IEEE Transactions, 51, pp. 778-789; 
Suzuki, K., Shiraishi, J., Abe, H., MacMahon, H., Doi, K., False-positive reduction in computer-aided diagnostic scheme for detecting nodules in chest radiographs by means of massive training artificial neural network (2005) Academic Radiology, 12, pp. 191-201; 
Schilham, A., van Ginneken, B., Loog, M., Multi-scale Nodule Detection in Chest Radiographs (2003) LNCS, 2878, pp. 602-609. , Ellis, R.E, Peters, T.M, eds, MICCAI 2003, Springer, Heidelberg; 
Schilham, A., van Ginneken, B., Loog, M., A computer-aided diagnosis system for detection of lung nodules in chest radiographs with an evaluation on a public database (2006) Medical Image Analysis, 10, pp. 247-258; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Transactions on Image Processing, 8 (8); 
Shiraishi, J., Katsuragawa, S., Kezoe, S., Matsumoto, T., Kobayashi, T., Komatsu, K., Matsui, M., Doi, K., Development of a Digital Image Database for Chest Radiographs With and Without a Lung Nodule: Receiver Operating Characteristic Analysis of Radiologists{\textquoteright} Detection of Pulmonary Nodules (2000) American Journal of Roentgenology, 174, pp. 71-74; 
Kobatake, H., Murakami, M., Adaptive filter to detect rounded convex regions: Iris filter (1996) Int. Conf. Pattern Recognition, 11, pp. 340-344; 
Wei, J., Hagihara, Y., Kobatake, H.: Detection of cancerous tumors on chest X-ray images - Candidate detection filter and its evaluation, ICIP99, no. 27AP4.2 (1999)Kobatake, H., Wei, J., Yoshinaga, Y., Hagihara, Y., Shimizo, A., Nonlinear Adaptine Convergence Index Filters and Their Characteristics (2000) Int. Conf. Pattern Recognition, pp. 522-525
},
	month = {2007///},
	pages = {170 - 177},
	address = {Girona},
	abstract = {This paper presents an automated method for the selection of a set of lung nodule candidates, which is the first stage of a computeraided diagnosis system for the detection of pulmonary nodules. An innovative operator, called sliding band filter (SBF), is used for enhancing the lung field areas. In order to reduce the influence of the blood vessels near the mediastinum, this filtered image is multiplied by a mask that assigns to each lung field point an a priori probability of belonging to a nodule. The result is further processed with a watershed segmentation method that divides each lung field into a set of non-overlapping areas. Suspicious nodule locations are associated with the regions containing the highest regional maximum values. The proposed method, whose result is an ordered set of lung nodule candidate regions, was evaluated on the 247 images of the JSRT database with very promising results. {\textcopyright} Springer-Verlag Berlin Heidelberg 2007.},
	keywords = {Blood Vessels, Computer aided diagnosis, Database systems, Lung nodule candidates, Non-overlapping areas, Pulmonary diseases, Pulmonary nodules, Radiography, Sliding band filter (SBF)},
	isbn = {03029743 (ISSN); 9783540728481 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38049105791\&partnerID=40\&md5=9af5e55fe146cd0455d2302e5383e14c},
	author = {Pereira, C.S. and Fernandes, H. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@conference {2370,
	title = {Efficient approximation of the mahalanobis distance for tracking with the Kalman filter},
	booktitle = {Proceedings of the International Symposium CompIMAGE 2006 - Computational Modelling of Objects Represented in Images: Fundamentals, Methods and ApplicationsProc. Int. Symp. CompIMAGE - Comput. Model. Objects Represent. Images: Fundam., Methods Appl.},
	series = {International Symposium CompIMAGE 2006 - Computational Modelling of Objects Represented in Images: Fundamentals, Methods and Applications},
	year = {2007},
	note = {Conference code: 75470Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pinho, R. R.; FEUP- Faculdade de Engenharia da Universidade do PortoPortugalReferences: Brown, L., Senior, A., Tian, Y., Connell, J., Hampapur, A., Shu, C., Merkl, H., Lu, M., Performance evaluation of surveillance systems under varying conditions (2005) IEEE International Workshop on Performance Evaluation of Tracking and Surveillance, , Colorado, USA;Cucchiara, R., Grana, C., Piccardi, M., Prati, A., Statistic and knowledge-based moving object detection in traffic scenes (2000) IEEE Conference on Intelligent Transportation Systems, , Dearborn, USA; 
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Lin, L., Bar-Shalom, Y., New assignment-based data association for tracking move-stop-move targets (2004) IEEE Transactions on Aerospace and Electronic Systems, 40 (2), pp. 714-725; 
O{\textquoteright}Malley, P., Nechyba, M., Arroyo, A., Human activity tracking for wide-area surveillance (2002) 2002 Florida Conference on Recent Advances in Robotics, , Miami, USA; 
Pinho, R., Tavares, J., Correia, M., Human movement tracking and analysis with kalman filtering and global optimization techniques (2005) II International Conference On Computational Bioengineering, , Lisbon, Portugal; 
Sun, F., Omachi, S., Kato, N., Aso, H., Kono, S., Takagi, T., Two-stage computational cost reduction algorithm based on mahalanobis distance approximations (2000) International Journal of Pattern Recognition, 2, pp. 2969-2973; 
Welch, G., Bishop, G., An Introduction to Kalman Filter (1995), Technical report, University of North Carolina at Chapel HillWren, C., Azarbayejani, A., Darell, T., Pentland, A., Pfinder: Real-time tracking of the human body (1997) IEEE Transactions on Pattern Analysis and Machine Intelligence, 19 (1), pp. 780-785
},
	month = {2007///},
	pages = {349 - 354},
	address = {Coimbra},
	abstract = {We address the problem of tracking efficiently feature points along image sequences. To estimate the undergoing movement we use an approach based on Kalman filtering which performs the prediction and correction of the features movement in every image frame. In this paper measured data is incorporated by optimizing the global correspondence set based on efficient approximations of the Mahalanobis distances (MD). We analyze the difference between using the MD and its efficient approximation in the tracking results, and also examine the related computational costs. Experimental results which validate our approach are presented. {\textcopyright} 2007 Taylor \& Francis Group.},
	keywords = {Computational costs, Control theory, Feature points, Image frames, Image sequences, Kalman filters, Kalman-filtering, Mahalanobis distances, Measured datum},
	isbn = {9780415433495 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-60749099808\&partnerID=40\&md5=4b296b2717f949790bc5897bd42c5950},
	author = {Pinho, R.R. and Tavares, J.M.R.S. and Correia, M.V.}
}
@article {2379,
	title = {Efficient approximation of the mahalanobis distance for tracking with the Kalman filter},
	journal = {International Journal of Simulation ModellingInt. J. Simul. Model.},
	volume = {6},
	year = {2007},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.2507/IJSIMM06(2)S.03Language of Original Document: EnglishCorrespondence Address: Pinho, R.R.; FEUP - Faculdade de Engenharia da Universidade do Porto, INEGI - Instituto de Engenharia. Mec{\^a}nica e Gest{\~a}o Industrial, LOMe - Lab. de {\'O}ptica e Mec{\r a}nica ExperimentalPortugal; email: rpinho@fe.up.ptReferences: Wren, C., Azarbayejani, A., Darrell, T., Pentland, A., Pfinder: Real-Time Tracking of the Human Body (1997) IEEE Transactions on Pattern Analysis and Machine Intelligence, 19 (7), pp. 780-785;Cucchiara, R., Grana, C., Piccardi, M., Prati, A., Statistic and Knowledge-based Moving Object Detection in Traffic Scenes (2000) Proceedings of the 3rd IEEE Conference on Intelligent Transportation Systems (ITSC2000), , Dearborn, Indiana, USA; 
Jorge, P., Abrantes, A., Marques, J., On-line Tracking Groups of Pedestrians with Bayesian Networks (2004) Workshop PETS, , Prague, Czech Republic; 
Lin, L., Bar-Shalom, Y., New Assignment-Based Data Association for Tracking Move-Stop-Move Targets (2004) IEEE Transactions on Aerospace and Electronic Systems, 40 (2), pp. 714-725; 
O{\textquoteright}Malley, P., Nechyba, M., Arroyo, A., Human Activity Tracking for Wide-Area Surveillance (2002) 2002 Florida Conference on Recent Advances in Robotics, , Miami, USA; 
Kurita, M., Tsuruoka, S., Yokoi, S., Miyak{\'e}, Y., Handprinted "Kanji" and "Hiragana" character recognition using weighting direction index histograms and quasi-Mahalanobis distance (1983) IEICE Technical Report, , PRL82-79; 
Kato, N., Abe, M., Nemoto, Y., A Handwritten Character Recognition System Using Modified Mahalanobis Distance (1996) Transactions Institute of Electronics, Information and Communication Engineers (IEICE), J79-D (L), pp. 45-52; 
Kimura, F., Shridhar, M., Handwritten numerical recognition based on multiple algorithms (1991) Pattern Recognition, 24 (10), pp. 969-983; 
Sun, F., Omachi, S., Kato, N., Aso, H., Kono, S., Takagi, T., Two-Stage Computational Cost Reduction Algorithm Based on Mahalanobis Distance Approximations (2000) International Conference of Pattern Recognition, , Barcelona, Spain; 
Pinho, R., Tavares, J., Correia, M., Human Movement Tracking and Analysis with Kalman Filtering and Global Optimization Techniques (2005) II International Conference On Computational Bioengineering, , Lisbon, Portugal; 
Welch, G., Bishop, G., An Introduction to Kalman Filter (1995), University of North Carolina at Chapel Hill, Technical Report, TR 95-041Pinho, R., Tavares, J., Correia, M., A Movement Tracking Management Model with Kaiman Filtering, Global Optimization Techniques and Mahalanobis Distance (2005) ICCMSE 2005 International Conf. on Computational Methods in Science and Engineering, , Loutraki, Greece; 
Pinho, R., Tavares, J., Correia, M., An Improved Management Model for Tracking Missing Features in Computer Vision Long Image Sequences (2007) WSEAS Transactions on Information Science and Applications, 4 (1), pp. 196-203; 
Brown, L., Senior, A.W., Tian, Y., Connell, J., Hampapur, A., Shu, C., Merkl, H., Lu, M., Performance Evaluation of Surveillance Systems Under Varying Conditions (2005) IEEE Int. Workshop on Performance Evaluation of Tracking and Surveillance, , Colorado, USA
},
	month = {2007///},
	pages = {84 - 92},
	abstract = {In this paper, we address the problem of tracking feature points along image sequences efficiently. Thus, to estimate the undergoing movement we use an approach based on Kalman filtering, which performs the prediction and correction of the features{\textquoteright} movement in every image frame. Measured data is incorporated by optimizing the global association set built on efficient approximations of the Mahalanobis distance (MD). We analyze the difference between the usage in the tracking results of the original MD formulation and its more efficient approximation, as well as the related computational costs. Experimental results which validate our approach are presented.},
	keywords = {Computational vision, Data association, Kalman filter, Mahalanobis distance, Tracking},
	isbn = {17264529 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34250711808\&partnerID=40\&md5=1c5bde5729463ffbefb5dacc6bacf9fd},
	author = {Pinho, R.R. and Tavares, J.M.R.S. and Correia, M.V.}
}
@article {2380,
	title = {Epidemiology of femur fractures in Portugal},
	journal = {Epidemiologia das fracturas do f{\'e}mur em Portugal: Fracturas do colo do f{\'e}mur versus fracturas de outras localiza{\c c}{\~o}es n{\~a}o especificadas do f{\'e}murArquivos de MedicinaArq. Med.},
	volume = {21},
	year = {2007},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: ARQMELanguage of Original Document: PortugueseCorrespondence Address: Alves, S.F.; Laborat{\'o}rio de Biomateriais, Instituto de Engenharia Biom{\'e}dica (INEB), Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: smfalves@gmail.comReferences: Aroso-Dias, A., Epidemiologia da Osteoporose em Portugal: An{\'a}lise comparative com outros pa{\'\i}ses. (2000) Acta Reumatol Port, 97, pp. 21-31;De Laet, C.E.D.H., Pols, H.A.P., Fractures in the elderly: Epidemiology and demography (2000) Baillieres Clin Endocrinol Metab, 14, pp. 171-179; 
Eisman, J., The epidemiology and pathogenesis of osteoporosis (1997) Baillieres Clin Endocrinol Metab, 11, pp. 23-62. , Center J; 
Dennison, E., Cooper, C., Epidemiology of Osteoporotic Fractures (2000) Horm Res, 54, pp. 58-63; 
Amin, S., Felson, D.T., Osteoporosis in Men (2001) Rheum Dis Clin North Am, 27, pp. 19-47; 
Jordan, K.M., Cooper, C., Epidemiology of osteoporosis (2002) Best Pract Res Clin Rheumatol, 16, pp. 795-806; 
Kanis, J.A., Diagnosis of osteoporosis and assessment of fracture risk (2002) Lancet North Am Ed, 59, pp. 1929-1936; 
Cooper, C., Melton III, L.J., Magnitude and Impact of Osteoporosis and Fractures (1996) Osteoporosis, pp. 419-434. , Marcus R, Feldman D, Kelsey J, editors, Academic Press, San Diego;; 
Trombetti, A., Herrmann, F., Hoffmeyer, P., Schurch, M.A., Bonjour, J.P., Rizzoli, R., Survival and potential years of life lost after hip fracture in men and age-matched women (2002) Osteoporos Int, 13, pp. 731-737; 
O{\textquoteright}Neill, T.W., Roy, D.K., The epidemiology and scale of the problem (2003) Hosp Med, 64, pp. 517-520; 
Cummings, S.R., Melton III, L.J., Epidemiology and outcomes of osteoporotic fractures (2002) Lancet, 359, pp. 1761-1767; 
www.ine.pt, Instituto Nacional de EstatisticaWalker-Bone, K., Dennison, E., Cooper, C., Epidemiology of Osteoporosis (2001) Rheum Dis Clin North Am, 27, pp. 1-18; 
Gold, D.T., The Nonskeletal Consequences of Osteoporotic Fractures - Psycologic and Social Outcomes (2001) Rheum Dis Clin North Am, 27, pp. 255-262
},
	month = {2007///},
	pages = {77 - 81+127},
	abstract = {Fractures related to osteoporosis have been increasing in many countries. Femoral neck fractures have been widely studied, however little is known about other femur fractures and its relationship with osteoporosis. The main objective of this study is the comparison between the incidence of neck fractures attributed to osteoporosis with the ones that occurs in other places of femur other than the neck, in the same conditions. It was used a national database, under the regulation of a governmental institute, with registers from 2000 to 2002. From the total number of femural fractures the authors selected the registers corresponding to individuals with more than fifty years of age and due to low impact, it were analysed separately the femoral neck fractures (International Classification of Diseases codes, 9                        th Revision, Clinical Modification, ICD9-CM codes 820 and derivates) from the fractures that occurred in other locations in the femur (ICD9-CM codes 821 and derivates). Case fatality rates and the incidence rates were calculated for that period of time, and an analysis of the variables age, days and costs of hospital admission as well as of the variable destination after discharge. Only 7.6\% of the femural fractures were coded as 821.x, all the others were coded as 820.x. In both groups the fractures were more frequent in women (above 75\%) and showed an exponential increase with age. The costs were higher in the 820.x fractures which also presented higher case fatality rates. {\textcopyright}ArquiMed, 2007.
},
	keywords = {adult, aged, article, Female, femur, femur fracture, fracture, hip fracture, human, incidence, Low impact, male, Osteoporosis, outcome assessment, Portugal, risk assessment, survival},
	isbn = {08713413 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38949187951\&partnerID=40\&md5=bb28b6850d1e7d2f67a7f0c9be4c8ff9},
	author = {Alves, S.F. and Pina, M.D.F. and Barbosa, M.}
}
@book {2372,
	title = {Evaluation of contrast enhancement filters for lung nodule detection},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4633 LNCS},
	year = {2007},
	note = {Conference code: 70855Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pereira, C.S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Sinal e Imagem, Campus FEUP (Faculdade de Engenharia da Universidade do Porto), Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: cmsp@fe.up.ptReferences: Kobatake, H., Murakami, M., Adaptive filter to detect rounded convex regions: Iris filter (1996) Int. Conf. Pattern Recognition, 2, pp. 340-344;Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Transactions on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H.: Detection of cancerous tumors on chest X-ray images - Candidate detection filter and its evaluation. In: ICIP99, 27AP4.2 (1999)Kobatake, H., Wei, J., Yoshinaga, Y., Hagihara, Y., Shimizo, A., Nonlinear Adaptinc Convergence Index Filters and Their Characteristics (2000) Int. Conf. Pattern Recognition, pp. 522-525; 
Shiraishi, J., Katsuragawa, S., Kezoe, S., Matsumoto, T., Kobayashi, T., Komatsu, K., Matsui, M., Doi, K., Development of a Digital Image Database for Chest Radiographs With and Without a Lung Nodule: Receiver Operating Characteristic Analysis of Radiologists{\textquoteright} Detection of Pulmonary Nodules (2000) American Journal of Roentgenology, 174, pp. 71-74; 
Keserci, B., Yoshida, H., Computerized detection of pulmonary nodules in chest radiographs based on morphological features and wavelet snake model (2002) Medical Image Analysis, 6, pp. 431-447; 
Song, X., Pogue, B., Jiang, S., Doyley, M., Dehghani, H., Tosteson, T., Paulsen, K., Automated region detection based on the contrast-to-noise ratio in near-infrared tomography (2004) Applied Optics, 43 (5), pp. 1053-1062; 
Singh, S., Bovis, K., An evaluation of contrast enhancement techniques for mammographic breast masses (2005) IEEE Transactions On Information Technology in Biomedicine, 9 (1), pp. 109-119; 
Wei, J., Hagihara, Y., Shimizu, A., Kobatake, H., Optimal image feature set for detecting lung nodules on chest X-ray images (2002) CARS; 
Suzuki, K., Shiraishi, J., Abe, H., MacMahon, H., Doi, K., False-positive reduction in computer-aided diagnostic scheme for detecting nodules in chest radiographs by means of massive training artificial neural network (2005) Academic Radiology, 12, pp. 191-201; 
},
	month = {2007///},
	pages = {878 - 888},
	address = {Montreal},
	abstract = {The aim of this paper is to evaluate and compare the performance of three convergence index (CI) filters when applied to the enhancement of chest radiographs, aiming at the detection of lung nodules. One of these filters, the sliding band filter (SBF), is the proposal of an innovative operator, while the other two CI class members, the iris filter (IF) and the adaptive ring filter (ARF), are already known in this application. To demonstrate the adequacy of the new filter for the enhancement of chest x-ray images, we calculated several figures of merit with the goal of comparing (i) the contrast enhancement capability of the filters, and (ii) the behavior of the filters for the detection of lung nodules. The results obtained for 154 images with nodules of the JSRT database show that the SBF outperforms both the IF and ARF. The proposed filter demonstrated to be a promising enhancement method, thus justifying its use in the first stage of a computer-aided diagnosis system for the detection of lung nodules. {\textcopyright} Springer-Verlag Berlin Heidelberg 2007.},
	keywords = {Adaptive systems, Contrast enhancement filters, Database systems, Image segmentation, Iris filter (IF), Medical imaging, Radiography, X rays},
	isbn = {03029743 (ISSN); 9783540742586 (ISBN)},
	author = {Pereira, C.S. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2387,
	title = {An improved management model for tracking missing features in computer vision long image sequences},
	journal = {WSEAS Transactions on Information Science and ApplicationsWSEAS Trans. Inf. Sci. Appl.},
	volume = {4},
	year = {2007},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pinho, R.R.; FEUP - Faculdade de Engenharia da Universidade do Porto, INEGI - Instituto de Engenharia Mec{\^a}nica e Gest{\~a}o Industrial, LOME - Lab. de {\'O}ptica e Mec{\^a}nica Experimental, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal; email: rpinho@fe.up.ptReferences: Davison, A., Deutscher, J., Reid, I., "Markerless motion Capture of Complex Full-Body Movement for Character Animation" (2001) Proceedings Eurographics Workshop on Animation and Simulation, , Manchester, UK;Lakany, H., Hayes, G., Hazlewood, M., Hillman, S., "Human Walking: Tracking and Analysis" (1999) IEEE Electronic \& Communications, Colloquium on Motion Analysis and Tracking; 
Rosales, R., Sclaroff, S., "Improved Tracking of Multiple Humans with Trajectory Prediction and Occlusion Modelling" (1998) Proceedings IEEE Conference on Computer Vision and Pattern Recognition, Workshop on the Interpretation of Visual Motion, , Santa Barbara, USA; 
Huang, Y., Essa, I., "Tracking Multiple Objects through Occlusions" (2005) IEEE Conference on Computer Vision and Pattern Recognition 2005 (CVPR 2005), , San Diego, USA; 
Zhao, T.S., Nevatia, R., "Tracking Multiple Humans in Complex Situations" (2004) IEEE Transactions on Image Processing, 26 (9), pp. 1208-1221; 
Pl{\"a}nkers, R., Fua, P., "Tracking and Modeling People in Video Sequences" (2001) Computer Vision and Image Understanding, 81 (3), pp. 285-302; 
Jorge, P., Abrantes, A., Marques, J., "On-line Tracking Groups of Pedestrians with Bayesian Networks" (2004) Workshop PETS, , Prague, Czech Republic; 
Wren, C., Azarbayejani, A., Darrell, T., Pentland, A., "Pfinder: Real-Time Tracking of the Human Body" (1997) IEEE Transactions on Pattern Analysis and Machine Intelligence, 19 (7), pp. 780-785; 
Tavares, J., Padilha, A., "Matching Lines in Image Sequences with Geometric Constraints" (1995) 7{\textdegree} Congresso Portugu{\^e}s De Reconhecimento De Padr{\~o}es, , Aveiro, Portugal; 
Pinho, R., Tavares, J., Correia, M., "A Movement Tracking Management Model with Kalman Filtering, Global Optimization Techniques and Mahalanobis Distance" (2005) ICCMSE 2005 - International Conference on Computational Methods in Science and Engineering, , Loutraki, Greece; 
Baumol, W., (1985) Economic Theory and Operations Research, , Prentice-Hall; 
Levy, H., (1994) Capital Investment and Financial Decisions, , Prentice-Hall; 
Pinho, R., Tavares, J., Correia, M., "Human Movement Tracking and Analysis with Kalman Filtering and Global Optimization Techniques" (2005) II International Conference On Computational Bioengineering, , Lisbon, Portugal; 
},
	month = {2007///},
	pages = {196 - 202},
	abstract = {In this paper we present a management model to deal with the problem of tracking missing features during long image sequences using Computational Vision. Some usual difficulties related with missing features are that they may be temporarily occluded or might even have disappeared definitively, and the computational cost involved should always be reduced to the strictly necessary. The proposed Net Present Value (NPV) model, based on the economic Theory of Capital, considers the tracking of each missing feature as an investment. Thus, using the NPV criterion, with adequate receipt and outlay functions, each occluded feature may be kept on tracking or it may be excluded of the tracking process depending on its historical behavior. This approach may be applied to any tracking system as long as the tracking results may be evaluated in each temporal step, and it can deal with the appearance, occlusion and disappearance of features especially useful for tracking in long image sequences. Experimental results, both on synthetic and real image sequences, which validate our model, will be also presented.},
	keywords = {Automatic target recognition, Computer vision, Feature extraction, Long image sequences, Management, Mathematical models, Missing features, Motion estimation, Net Present Value (NPV), Occlusion, Tracking},
	isbn = {17900832 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34250734547\&partnerID=40\&md5=591b93181afd5b31c2211f092b2cefb0},
	author = {Pinho, R.R. and Tavares, J.M.R.S. and Correia, M.V.}
}
@article {2375,
	title = {Linear and nonlinear analysis of heart rate patterns associated with fetal behavioral states in the antepartum period},
	journal = {Early Human DevelopmentEarly Hum. Dev.},
	volume = {83},
	year = {2007},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: ScopusCODEN: EHDEDdoi: 10.1016/j.earlhumdev.2006.12.006PubMed ID: 17261357Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, H.; Universidade do Porto, Faculdade de Ci{\^e}ncias, Departamento de Matem{\'a}tica Aplicada, Porto, Portugal; email: hernani.goncalves@fc.up.ptReferences: Nijhuis, J.G., Prechtl, H.F., Martin, C.B.J., Bots, R.S., Are there behavioural states in the human fetus? (1982) Early Hum Dev, 6, pp. 177-195;Nijhuis, I., ten Hof, J., Development of fetal heart rate and behavior: indirect measures to assess the fetal nervous system (1999) Eur J Obstet Gynaecol Reprod Biol, 87, pp. 1-2; 
Vindla, S., James, D., Fetal behaviour as a test of fetal wellbeing (1995) Br J Obstet Gynaecol, 102, pp. 597-600; 
Swartjes, J.M., van Geijn, H.P., Mantel, R., van Woerden, E.E., Schoemaker, H.C., Coincidence of behavioural state parameters in the human fetus at three gestational ages (1990) Early Hum Dev, 23, pp. 75-83; 
Ayres-de-Campos, D., Santos, C., Bernardes, J., Prediction of neonatal state by computer analysis of fetal heart rate tracings: the antepartum arm of the SisPorto{\textregistered} multicentre validation study (2005) Eur J Obstet Gynaecol Reprod Biol, 118, pp. 52-60; 
Ayres-de-Campos, D., Bernardes, J., Garrido, A., de S{\'a}, J.M., Pereira-Leite, L., SisPorto 2.0: a program for automated analysis of cardiotocograms (2000) J Matern Fetal Med, 9, pp. 311-318; 
Rooth, G., Huch, A., Huch, R., FIGO news. Guidelines for the use of fetal monitoring (1987) Int J Gynaecol Obstet, 25, p. 159; 
Bernardes, J., Moura, C., de S{\'a}, J.P.M., Pereira-Leite, L., The Porto system for automated cardiotocographic signal analysis (1991) J Perinat Med, 19, pp. 61-65; 
Gon{\c c}alves, H., Rocha, A.P., Ayres-de-Campos, D., Bernardes, J., Internal versus external intrapartum foetal heart rate monitoring: the effect on linear and nonlinear parameters (2006) Physiol Meas, 27, pp. 307-319; 
Signorini, M.G., Magenes, G., Cerrutti, S., Arduini, D., Linear and nonlinear parameters for the analysis of fetal heart rate signal from cardiotacographic recordings (2003) IEEE Trans Biomed Eng, 50 (3), pp. 365-373; 
Pincus, S., Viscarello, R., Approximate Entropy: a regularity measure for fetal heart rate analysis (1992) Obstet Gynaecol, 79 (2), pp. 249-255; 
Richman, J.S., Moorman, J.R., Physiological time-series analysis using approximate entropy and sample entropy (2000) Am J Physiol Heart Circ Physiol, 278, pp. H2039-H2049; 
Pincus, S., Approximate entropy (ApEn) as a complexity measure (1995) Chaos, 5 (1), pp. 110-117; 
Grant, J.M., The fetal heart rate trace is normal, isn{\textquoteright}t it? Observer agreement of categorical assessments (1991) Lancet, 337, pp. 215-218; 
Martinez, W.L., Martinez, A.R., (2002) Computational statistics handbook with MATLAB, , CRC Press; 
van Woerden, E.E., Geijn, H.P., Caron, F.J., Swartjes, J.M., Mantel, R., Arts, N.F., Automated assignment of behavioural states in the human near term fetus (1989) Early Hum Dev, 19, pp. 137-146; 
Ten Hof, J., Nijhuis, I.J., Mulder, E.J., Nijhuis, J.G., Narayan, H., Taylor, D.J., Longitudinal study of fetal body movements: nomograms, intrafetal consistency, and relationship with episodes of heart rate patterns A and B (2002) Pediatr Res, 52 (4), pp. 568-575; 
Bernardes, J., Costa-Pereira, A., de Campos, D.A., van Geijn, H.P., Pereira-Leite, L., Evaluation of interobserver agreement of cardiotocograms (1997) Int J Gynaecol Obstet, 57 (1), pp. 33-37; 
Bernardes, J., Costa-Pereira, A., van Geijn, H.P., Pereira-Leite, L., A more objective fetal heart rate baseline estimation (1996) Br J Obstet Gynaecol, 103, pp. 714-715; 
Mantel, R., van Geijn, H.P., Ververs, I.A., Copray, F.J., Automated analysis of near-term antepartum fetal heart rate in relation to fetal behavioral states: the Sonicaid System 8000 (1992) Am J Obstet Gynaecol, 167 (6), pp. 1912-1914; 
Court, D.J., Parer, J.T., Experimental studies of fetal asphyxia and fetal heart rate interpretation (1984) Research in perinatal medicine, pp. 113-169. , Nathanielsz P.W., and Parer J.T. (Eds), Perinatology Press, New York; 
Oppenheimer, L.W., Lewinsky, R.M., Power spectral analysis of fetal heart rate (1994) Bailliere{\textquoteright}s Clin Obstet Gynaecol, 8 (3), pp. 643-661; 
van Woerden, E.E., Geijn, H.P., Caron, F.J., Mantel, R., Spectral analysis of fetal heart rhythm in relation to fetal regular mouthing (1990) Int J Biomed Comput, 25 (4), pp. 253-260; 
Boog, G., Analyse informatis{\'e}e du rythme cardiaque foetal au cours de la grossesse et de l{\textquoteright}accouchement (2004) Encycl M{\'e}d-Chir, 148 (5-49 -D-23), pp. 1-10; 
Suzuki, T., Okamura, K., Kimura, Y., Watanabe, T., Murotsuki, N.Y.J., Uehara, S., Power spectral analysis of R-R interval variability before and during the sinusoidal heart rate pattern in fetal lambs (2000) Am J Obstet Gynaecol, 182, pp. 1227-1232; 
Dawes, G.S., Moulden, M., Sheil, O., Redman, C.W.G., Approximate entropy, a statistic of regularity, applied to fetal heart rate data before and during labour (1992) Obstet Gynaecol, 80 (5), pp. 763-768; 
Magenes, G., Signorini, M.G., Arduini, D., Cerutti, S., Fetal heart rate variability due to vibroacoustic stimulation: linear and nonlinear contribution (2004) Methods Inf Med, 43 (1), pp. 47-51; 
Felgueiras, C., de S{\'a}, J.P.M., Bernardes, J., Gama, S., Fractal characterisation of fetal heart rate sequences (1998) Biomed Eng, 36 (2), pp. 197-201; 
Vieira, P., Marques-de-S{\'a}, J.P., Bernardes, J., Fractal analysis of foetal heart rate (2004) Proceedings of biosignal 2004, pp. 53-55. , Jan J., Kozumpl{\'\i}k J., and Provazn{\'\i}k I. (Eds), Vutium Press, Brno University of Technology; 
di Renzo, G.C., Montani, M., Fioriti, V., Clerici, G., Branconi, F., Pardini, A., Fractal analysis: a new method for evaluating fetal heart rate variability (1996) J Perinat Med, 24 (3), pp. 261-269
},
	month = {2007///},
	pages = {585 - 591},
	abstract = {Background: Fetal behavioral states are important indicators of fetal physiology and pathology associated to typical fetal heart rate (FHR) patterns. Aim: To provide linear and nonlinear analysis of FHR patterns associated with fetal behavioral states regarding a better understanding of these states and patterns. Study design and subjects: Fifty FHR tracings from normal term pregnancies with a median duration of 40.3~min were acquired with the SisPorto{\textregistered} 2.01 system for computerized analysis of cardiotocograms. Each tracing was divided into consecutive 10-minute segments and each segment was classified by two experts as pattern A, B, C or D. Outcome measures: Linear and nonlinear indices were computed in each segment, namely mean FHR, long-term irregularity index (LTI), very low (VLF), low (LF) and high (HF) frequency spectral indices, approximate entropy (ApEn) and sample entropy (SampEn). Kappa statistic (κ) and proportions of agreement (Pa) were used for assessment of inter-observer agreement. Bootstrap percentile confidence intervals and nonparametric statistical tests were calculated for statistical inference. Results: Overall agreement between experts in pattern classification was good to excellent with values for κ and Pa of 0.74 (95\% CI: 0.64-0.94) and 0.94 (95\% CI: 0.92-0.96), respectively. Most linear domain indices increased significantly with rising fetal activity whereas the opposite occurred with nonlinear indices, except for SampEn(2, 0.1). LF/(MF + HF) ratio also significantly increased with fetal activity, denoting an increased sympatho-vagal balance. Conclusions: Results support the hypothesis that entropy and linear variability indices measure different FHR features. FHR patterns associated with active sleep (B) and active wakefulness (D) evidenced more signs of autonomous nervous system activity, with sympatho-vagal imbalance, and less signs related to complexity or irregularity control systems than patterns associated with calm sleep (A) and calm wakefulness (C). {\textcopyright} 2006 Elsevier Ireland Ltd. All rights reserved.},
	keywords = {adult, article, behavior change, Cardiotocography, Computerized analysis, controlled study, entropy, Female, Fetal behavioral states, Fetal heart rate, Fetal monitoring, fetus, fetus heart rate, fetus monitoring, Heart Rate, Fetal, human, Humans, Image Interpretation, Computer-Assisted, Linear Models, Nonlinear Dynamics, Pregnancy, SisPorto, Spectral analysis, vagus nerve stimulation},
	isbn = {03783782 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249751815\&partnerID=40\&md5=3a787d5accf317ad2b020e59ddb6a465},
	author = {Gon{\c c}alves, H. and Bernardes, J. and Paula Rocha, A. and Ayres-de-Campos, D.}
}
@book {2384,
	title = {Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 I},
	year = {2007},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Pataquiva Mateus, A.Y.; INEB - Institute de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptReferences: Liou, S., Chen, S., Liu, D., (2003) Biomaterials, 24, p. 3981;Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., (1999) Journal of Biomedical Materials Research, 47, p. 28; 
Grimaldi, G., Weiss, P., Millot, F., Daculsi, G., (1998) Journal of Biomedical Materials Research, 39, p. 660; 
Termenoff, J., Mikos, A., (2000) Biomaterials, 21, p. 2405; 
Best, S., Bonfield, W., Doyle, C., (1989) Bioceramics, p. 68. , Oonishi H, Aoki H, Sawai K, editors, Tokyo: Ishiyaku Euro-America; 
Pataquiva-Mateus, A.Y., Ferraz, M.P., Monteiro, F.J., Journal of Biomedical Materials Research, , Submitted paper
},
	month = {2007///},
	pages = {243 - 246},
	abstract = {This study concerns the preparation and characterisation of microspheres associating alginate and two different types of hydroxyapatite (HA), which are intended to be used as drug delivery systems and bone regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using a chemical precipitation synthesis based on H3PO4, Ca(OH)2 and a surfactant, SDS (sodium dodecylsulphate), as starting reagents. These two powders of nanoHA and alginate were used to prepare two different types of microspheres. Both powders and microspheres were characterised using FTIR, TEM, SEM, mercury porosimetry analysis and X-ray diffraction Results show that pure hydroxyapatite (HA) and mixtures of HA/β-TCP in the nanometre range were obtained from both HA syntheses. Microspheres with different characteristics were obtained from these two types of hydroxyapatite.},
	keywords = {Alginate, Bone, Bone Regeneration, Chemical precipitation, Controlled drug delivery, Fourier transform infrared spectroscopy, hydroxyapatite, Microspheres, Microstructure, nanoparticles, Nanostructured materials, Scanning electron microscopy, Surface active agents, transmission electron microscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846333853\&partnerID=40\&md5=f694c3d331dea2c82aba11cef4dddd59},
	author = {Pataquiva Mateus, A.Y. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2394,
	title = {An improved management model for tracking multiple features in long image sequences},
	journal = {WSEAS Transactions on Information Science and ApplicationsWSEAS Trans. Inf. Sci. Appl.},
	volume = {3},
	year = {2006},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pinho, R.R.; FEUP - Faculdade de Engenharia da Universidade do Porto, INEGI - Instituto de Engenharia Mec{\^a}nica e Gest{\~a}o Industrial, LOME - Lab. de {\'O}ptica e Mec{\^a}nica Experimental, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal; email: rpinho@fe.up.ptReferences: Davison, A., Deutscher, J., Reid, I., "Markerless motion Capture of Complex Full-Body Movement for Character Animation" (2001) Proceedings Eurographics Workshop on Animation and Simulation, , Manchester;Lakany, H., Hayes, G., Hazlewood, M., Hillman, S., "Human Walking: Tracking and Analysis" (1999) IEEE Electronic \& Communications, Colloquium on Motion Analysis and Tracking; 
Rosales, R., Sclaroff, S., "Improved Tracking of Multiple Humans with Trajectory Prediction and Occlusion Modelling" (1998) Proceedings IEEE Conference on Computer Vision and Pattern Recognition Workshop on the Interpretation of Visual Motion, , Santa Barbara; 
Huang, Y., Essa, I., "Tracking Multiple Objects through Occlusions" (2005) IEEE Conference on Computer Vision and Pattern Recognition 2005 (CVPR 2005), , San Diego, USA; 
Zhao, T., Nevatia, R., "Tracking Multiple Humans in Complex Situations" (2004) IEEE Transactions on Image Processing, 26, pp. 1208-1221; 
Pl{\"a}nkers, R., Fua, P., "Tracking and Modeling People in Video Sequences" (2001) Computer Vision and Image Understanding, 81, pp. 285-302; 
Jorge, P., Abrantes, A., Marques, J., "On-line Tracking Groups of Pedestrians with Bayesian Networks" (2004) Workshop PETS, , Prague, Czech Republic; 
Wren, C., Azarbayejani, A., Darrell, T., Pentland, A., "Pfinder: Real-Time Tracking of the Human Body" (1997) IEEE Transactions on Pattern Analysis and Machine Intelligence, 19, pp. 780-785; 
Tavares, J., Padilha, A., "Matching Lines in Image Sequences with Geometric Constraints" (1995) 7{\textdegree} Congresso Portugu{\^e}s De Reconhecimento De Padr{\~o}es, , Portugal; 
Pinho, R., Tavares, J., Correia, M., "A Movement Tracking Management Model with Kalman Filtering, Global Optimization Techniques and Mahalanobis Distance" (2005) ICCMSE 2005 - International Conference on Computational Methods in Science and Engineering, , Loutraki, Greece; 
Baumol, W., (1985) Economic Theory and Operations Research, , Prentice-Hall; 
Levy, H., (1994) Capital Investment and Financial Decisions, , Prentice-Hall; 
Pinho, R., Tavares, J., Correia, M., "Human Movement Tracking and Analysis with Kalman Filtering and Global Optimization Techniques" (2005) II International Conference on Computational Bioengineering, , Lisbon, Portugal; 
},
	month = {2006///},
	pages = {2165 - 2170},
	abstract = {In this paper we present a management model to deal with the problem of tracking a large number of features during long image sequences. Some usual difficulties are related to this problem: features may be temporarily occluded or might even have disappeared definitively; the computational cost involved should always be reduced to the strictly necessary. The proposed Net Present Value (NPV) model, based on the economic Theory of Capital, considers the tracking of each missing feature as an investment. Thus, using the NPV criterion, with adequate receipt and outlay functions, each occluded feature may be kept on tracking or it may be excluded of the tracking process depending on its historical behavior. This methodology may be applied to any tracking system as long as the tracking results may be evaluated in each temporal step. Experimental results, both on synthetic and real image sequences, which validate our model, will be also presented.},
	keywords = {Computer vision, Costs, Feature extraction, Image sequences, Imaging systems, Management, Management model, Motion estimation, Net Present Value (NPV), Occlusion, Tracking, Tracking (position), Tracking multiple features},
	isbn = {17900832 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749057299\&partnerID=40\&md5=9dfc702e9143511b35fb1b664f20d9e3},
	author = {Pinho, R.R. and Tavares, J.M.R.S. and Correia, M.V.}
}
@book {2397,
	title = {A multiclassifier approach for lung nodule classification},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4142 LNCS},
	year = {2006},
	note = {Conference code: 68310Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Pereira, C.S.; Instituto de Engenharia Biom{\'e}dica, Rua Roberto Frias, 4200-465 Porto, Portugal; email: cpereira@estgl.ipv.ptReferences: Jemal, A., Murray, T., Ward, E., Samuels, A., Tiwari, R.C., Ghafoor, A., Feuer, E.J., Thun, M.J., (2005) Cancer Statistics, 2005, 55, pp. 10-30. , CA Cancer J Clin;Keserci, B., Yoshida, H., Computerized detection of pulmonary nodules in chest radiographs based on morphological features and wavelet snake model (2002) Medical Image Analysis, 6, pp. 431-447; 
Van Ginneken, B., Romeny, B.M.H., Viergever, M.A., Computer-aided diagnosis in chest radiography: A survey (2001) IEEE Transactions on Medical Imaging, 20 (12), pp. 1228-1241; 
Wei, J., Hagihara, Y., Shimizu, A., Kobatake, H., Optimal image feature set for detecting lung nodules on chest X-ray images (2002) CARS 2002; 
Van Ginneken, B., Katsuragawa, S., Ter Haar Romeny, B.M., Kunio, D., Viergever, M.A., Automatic detection of abnormalities in chest radiographs using local texture analysis (2002) IEEE Transactions on Medical Imaging, 21, pp. 139-149; 
Schilham, A.M.R., Van Ginneken, B., Loog, M., Multi-scale nodule detection in chest radiographs (2003) Lecture Notes in Computer Science, LNCS 2878, pp. 602-609; 
Shiraishi, J., Katsuragawa, S., Ikezoe, J., Matsumoto, T., Kobayashi, T., Matsu, K.-I.K., Matsui, M., Doi, K., Development of a digital image database for chest radiographs with and without a lung nodule: Receiver operating characteristic analysis of radiologists{\textquoteright} detection of pulmonary nodules (2000) American Journal of Roentgenology, 174, pp. 71-74; 
Yoshida, H., Local contralateral subtraction based on bilateral symmetry of lung for reduction of false positives in computerized detection of pulmonary nodules (2004) IEEE Transactions on Biomedical Engineering, 51, pp. 778-789; 
Suzuki, K., Shiraishi, J., Abe, H., MacMahon, H., Doi, K., False-positive reduction in computer-aided diagnostic scheme for detecting nodules in chest radiographs by means of massive training artificial neural network (2005) Academic Radiology, 12, pp. 191-201; 
Randen, T., Husoy, J.H., Filtering for texture classification: A comparative study (1999) IEEE Transactions on Pattern Analysis and Machine Intelligence, 21 (4); 
Grigorescu, S.E., Petkov, N., Kruizinga, P., Comparison of texture features based on Gabor filters (2002) IEEE Transactions on Image Processing, 11 (10), pp. 195-203; 
Kumar, A., Pang, G.K.H., Defect detection in textured materials using Gabor filters (2002) IEEE Transactions on Industry Applications, 38 (2); 
Varma, M., Zisserman, A., Unifying statistical texture classification frameworks (2004) Image and Vision Computing, 22, pp. 1175-1183; 
Leung, T., Malik, J., Representing and recognizing the visual appearance of materials using three-dimensional textons (2001) International Journal of Computer Vision, 43, pp. 29-44; 
Alexandre, L.A., Campilho, A.C., Kamel, M., On combining classifiers using sum and product rules (2001) Pattern Recognition Letters, 22, pp. 1283-1289
},
	month = {2006///},
	pages = {612 - 623},
	address = {Povoa de Varzim},
	abstract = {The aim of this paper is to examine a multiclassifier approach to the classification of the lung nodules in X-ray chest radiographs. The approach investigated here is based on an image region-based classification whose output is the information of the presence or absence of a nodule in an image region. The classification was made, essentially, in two steps: firstly, a set of rotation invariant features was extracted from the responses of a multi-scale and multi-orientation filter bank; secondly, different classifiers (multi-layer perceptrons) are designed using different features sets and trained in different data. These classifiers are further combined in order to improve the classification performance. The obtained results are promising and can be used for reducing the false-positives nodules detected in a computer-aided diagnosis system. {\textcopyright} Springer-Verlag Berlin Heidelberg 2006.},
	keywords = {Biological organs, Classification (of information), Computer aided diagnosis, False-positives nodules, Medical imaging, Multi-orientation filter bank, Radiography, X rays, X-ray chest radiographs},
	isbn = {03029743 (ISSN); 3540448942 (ISBN); 9783540448945 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749658606\&partnerID=40\&md5=f6e83e868f5b1ee08b400ee9191138cd},
	author = {Pereira, C.S. and Alexandre, L.A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2407,
	title = {Silicon addition to hydroxyapatite increases nanoscale electrostatic, van der Waals, and adhesive interactions},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {78},
	year = {2006},
	note = {Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30737PubMed ID: 16646067Language of Original Document: EnglishCorrespondence Address: Ortiz, C.; Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States; email: coritz@mit.eduChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; silicon, 7440-21-3; thiol derivative, 13940-21-1; Biocompatible Materials; Durapatite, 1306-06-5; Silicon, 7440-21-3References: Carlisle, E.M., Silicon: A possible factor in bone calcification (1970) Science, 167, pp. 279-280;Gibson, I.R., Best, S.M., Bonfield, W., Chemical characterization of silicon-substituted hydroxyapatite (1999) J Biomed Mater Res, 44, pp. 422-428; 
Gibson, I.R., Best, S.M., Bonfield, W., Effect of silicon substitution on the sintering and microstructure of hydroxyapatite (2002) J Am Ceram Soc, 85, pp. 2771-2777; 
Legeros, R.Z., LeGeros, J.P., Dense hydroxyapatite (1993) An Introduction to Bioceramics, pp. 139-180. , Hench LL, Wilson J, editors. Singapore: World Scientific; 
Van Raemdonck, W., Ducheyne, P., De Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Biomaterials, pp. 143-166. , Ducheyne P, Hastings G, editors. Boca Raton, FL: CRC Press; 
Botelho, C.M., Lopes, M.A., Gibson, I.R., Best, S.M., Santos, J.D., Structural analysis of Si-substituted hydroxyapatite: Zeta potential and X-ray photoelectron spectroscopy (2002) J Mater Sci: Mater Med, 13, pp. 1123-1127; 
Gibson, I.R., Huang, J., Best, S.M., Bonfield, W., Enhanced in vitro cell activity and surface apatite layer formation on novel silicon-substituted hydroxyapatites (1999) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 12, pp. 191-194. , Ohgushi H, Hastings GW, Yoshikawa T, editors. River Edge, NJ: World Scientific Publishing; 
Balas, F., Perez-Pariente, J., Vallet-Regi, M., In vitro bioactivity of silicon-substituted hydroxyapatites (2003) J Biomed Mater Res A, 66, pp. 364-375; 
Botelho, C.M., Brooks, R.A., Best, S.M., Lopes, M.A., Santos, J.D., Rushton, N., Bonfield, W., Biological and physical-chemical characterization of phase pure HA and Si-substituted hydroxyapatite by different microscopy techniques (2004) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 16, pp. 845-848. , Barbosa MA, Monteiro FJ, Correia R, Leon B, editors. Uetikon-Zuerich, Switzerland: Trans Tech; 
Patel, N., Best, S.M., Bonfield, W., Gibson, I.R., Hing, K.A., Damien, E., Revel, P.A., A comparative study on the in vivo behavior of hydroxyapatite and silicon substituted hydroxyapatite granules (2002) J Mater Sci: Mater Med, 13, pp. 1199-1206; 
Porter, A.E., Patel, N., Skepper, J.N., Best, S.M., Bonfield, W., Effect of sintered silicate-substituted hydroxyapatite on remodeling process at the bone-implant interface (2004) Biomaterials, 25, pp. 3303-3314; 
Vandiver, J., Dean, D., Patel, N., Bonfield, W., Ortiz, C., Nanoscale variation in surface charge of synthetic hydroxyapatite detected by chemically and spatially specific high resolution force spectroscopy (2005) Biomaterials, 26, pp. 271-283; 
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Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, V.C.A., The ultrastructure of the bone-hydroxyapatite interface in vitro (1992) J Biomed Mater Res, 26, pp. 1365-1382; 
Neo, M., Nakamura, T., Ohtsuki, C., Kokubo, T., Yamamuro, T., Apatite formation on three kinds of bioactive material at an early stage in vivo: A comparative study by transmission electron microscopy (1993) J Biomed Mater Res, 27, pp. 999-1006; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 721-734; 
Himeno, T., Kim, H.-M., Kaneko, H., Kawashita, M., Kokubo, T., Nakamura, T., Surface structural changes of sintered hydroxyapatite in terms of surface charge (2003) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 15, pp. 457-460. , Besim Ben-Nissan DS, William Walsh, editors. Uetikon-Zuerich, Switzerland: Trans Tech; 
Sato, K., Kumagai, Y., Tanaka, J., Apatite formation on organic monolayers in simulated body environment (2000) J Biomed Mater Res, 50, pp. 16-20; 
Tanahashi, M., Matsuda, T., Surface functional group dependence on apatite formation on self-assembled monolayers in a simulated body fluid (1997) J Biomed Mater Res, 34, pp. 305-315; 
Yamashita, K., Oikawa, N., Umegaki, T., Acceleration and deceleration of bone-like crystal growth on ceramic hydroxyapatite by electric poling (1996) Chem Mater, 8, pp. 2697-2700; 
Hwang, K.S., Song, J.E., Jo, J.W., Yang, H.S., Park, Y.J., Ong, J.L., Rants, H.R., Effect of poling conditions on growth of calcium phosphate crystal in ferroelectric BaTiO 3 ceramics (2002) J Mater Sci: Mater Med, 13, pp. 133-138; 
Ohgaki, M., Kizuki, T., Katsura, M., Yamashita, K., Manipulation of selective cell adhesion and growth by surface charges of electrically polarized hydroxyapatite (2001) J Biomed Mater Res, 57, pp. 366-373; 
Kobayashi, T., Nakamura, S., Yamashita, K., Enhanced osteobonding by negative surface charges of electrically polarized hydroxyapatite (2001) J Biomed Mater Res, 57, pp. 477-484; 
Parsegian, V.A., Gingell, D., On the electrostatic interaction across a salt solution between two bodies bearing unequal charges (1972) Biophys J, 12, pp. 1192-1204; 
Butt, H.J., Measuring electrostatic, van der Waals, and hydration forces in electrolyte-solutions with an atomic force microscope (1991) Biophys J, 60, pp. 1438-1444; 
Brown, P.W., Martin, R.I., An analysis of hydroxyapatite surface layer formation (1999) J Phys Chem B, 103, pp. 1671-1675; 
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Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812; 
Seog, J., Dean, D., Plass, A.H.K., Wong-Palms, S., Grodzinsky, A.J., Ortiz, C., Direct measurement of glycosaminoglycan intermolecular interactions via high-resolution force spectroscopy (2002) Macromolecules, 35, pp. 5601-5615; 
Ashby, P.D., Chen, L., Lieber, C.M., Probing intermolecular forces and potentials with magnetic feedback chemical force microscopy (2000) J Am Chem Soc, 122, pp. 9467-9472; 
Ederth, T., Computation of Lifshitz-van de Waals forces between alkylthiol monolayers on gold films (2001) Langmuir, 17, pp. 3329-3340; 
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(1997) Principles of Colloid and Surface Chemistry, 3rd Ed., , Hiemenz P, Rajagopalan R, editors. New York: Marcel Dekker; 
Eichert, D., Combes, C., Drouet, C., Rey, C., Formation and evolution of hydrated surface layers of apatites (2005) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 17, pp. 3-6. , Li P, Zhang K, Colwell C, editors. Uetikon-Zuerich, Switzerland: Trans Tech; 
Rixman, M., Dean, D., Macias, C., Ortiz, C., Nanoscale intermolecular interactions between human serum albumin and alkanethiol self-assembled monolayers (2003) Langmuir, 19, pp. 6202-6218; 
Ackler, H.D., French, R.H., Chiang, Y., Comparisons of Hamaker constants for ceramic systems with intervening vacuum or water: From force laws and physical properties (1996) J Colloid Interface Sci, 179, pp. 460-469; 
Kane, V., Mulvaney, P., Double-layer interactions between self-assembled monolayers of ω-mercaptoundecanoic acid on gold surfaces (1998) Langmuir, 14, pp. 3302-3311; 
Atkins, P., (1997) The Elements of Physical Chemistry, , New York: Freeman; 
Zhu, P.X., Masuda, Y., Koumoto, K., Site-selective adhesion of hydroxyapatite microparticles on charged surfaces in a super-saturated solution (2001) J Colloid Interface Sci, 243, pp. 31-36; 
James, M., Hunter, R.J., O{\textquoteright}Brien, R.W., Effect of particle size distribution and aggregation on electroacoustic measurements of ξ potential (1992) Langmuir, 8, pp. 420-423; 
Toikka, G., Hayes, R.A., Ralston, J., Surface forces between spherical ZnS particles in aqueous electrolyte (1996) Langmuir, 12, pp. 3783-3788; 
Pedersen, H.G., Bergstrom, L., Forces measured between zirconia surfaces in poly(acrylic acid) solutions (1999) J Am Ceram Soc, 82, pp. 1137-1145; 
Cohen, M., Joester, D., Geiger, B., Addadi, L., Spatial and temporal sequence of events in cell adhesion: From molecular recognition to focal adhesion assembly (2004) Chem Bio Chem, 5, pp. 1393-1399; 
Smith, I.O., Baumann, M.J., McCabe, L.R., Electrostatic interactions as a predictor for osteoblast attachment to biomaterials (2004) J Biomed Mater Res A, 70, pp. 436-441; 
(1979) Plasma Proteins, , Blombaek B, Hanson L, editors. New York: Wiley; 
Porter, A.E., Hobbs, L.W., Rosen, V.B., Spector, M., The ultrastructure of the plasma-sprayed hydroxyapatite-bone interface predisposing to bone bonding (2002) Biomaterials, 23, pp. 725-733; 
Porter, A.E., Patel, N., Skepper, J.N., Best, S.M., Bonfield, W., Comparison of in vivo dissolution processes in hydroxyapatite and silicon-substituted hydroxyapatite bioceramics (2003) Biomaterials, 24, pp. 4609-4620
},
	month = {2006///},
	pages = {352 - 363},
	abstract = {The normal intersurface forces between nanosized probe tips functionalized with COO},
	keywords = {Adhesives, article, Atomic force microscopy, Biocompatible Materials, biomaterial, Biomaterials, Bone, cell adhesion, chemical binding, chemical interaction, derjaguin landau verwey overbeek approximation, Durapatite, electrochemical analysis, Electrostatic, Electrostatics, high resolution force spectroscopy, hydroxyapatite, materials testing, mathematical analysis, Microscopy, Electron, Scanning, Monolayers, Nanomechanics, Nanostructures, Particle Size, Poisson distribution, Polycrystalline materials, Silicon, Silicon-substituted hydroxyapatite, Spectrophotometry, Spectroscopy, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, Surface charge, surface property, thiol derivative, Van der Waals, Van der Waals forces},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33745965657\&partnerID=40\&md5=df44bade2fb0a3af786b193c7f42d71a},
	author = {Vandiver, J. and Dean, D. and Patel, N. and Botelho, C. and Best, S. and Santos, J.D. and Lopes, M.A. and Bonfield, W. and Ortiz, C.}
}
@article {2450,
	title = {In vitro degradation behavior of a novel bioresorbable composite material based on PLA and a soluble CaP glass},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {1},
	year = {2005},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2005.03.004PubMed ID: 16701822Language of Original Document: EnglishCorrespondence Address: Ginebra, M.P.; Reference Centre for Bioengineering of Catalonia (CREBEC), Department of Materials Science, Universitat Polit{\`e}cnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, Spain; email: maria.pau.ginebra@upc.esChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; polylactic acid, 26100-51-6; Bone Substitutes; calcium phosphate, 10103-46-5; Calcium Phosphates; Lactic Acid, 50-21-5; poly(lactic acid), 26100-51-6; PolymersManufacturers: Purac, NetherlandsReferences: Rokkanen, P., Bioabsorbable fixation in orthopaedic surgery and traumatology (2000) Biomaterials, 21, pp. 2607-2613;Middleton, J.C., Tipton, A.J., Synthetic biodegradable polymers as orthopedic devices (2000) Biomaterials, 21 (23), pp. 2335-2346; 
Adriano, K.P., Daniels, A.U., Smutz, W.P., Wyatt, R.W.B., Heller, J., Preliminary biocompatibility screening of several biodegradable phosphate fiber reinforced polymers (1993) J Appl Biomater, 4, pp. 1-12; 
Nazhat, S.N., Kellomaki, M., Tormala, P., Tanner, K.E., Bonfield, W., Dynamic mechanical characterization of biodegradable composites of hydroxyapatite and polylactides (2001) J Biomed Mater Res, 58 (4), pp. 335-343; 
Dupraz, A.M.P., de Wijn, J.R., v.d. Meer, S.A.T., de Groot, K., Characterization of silane-treated hydroxyapatite powders for use as filler in biodegradable composites (1996) J Biomed Mater Res, 30, pp. 231-238; 
Kasuga, T., Maeda, H., Kato, K., Nogami, M., Hata, K.-I., Ueda, M., Preparation of poly (lactic acid) composites containing calcium carbonate (vaterite) (2003) Biomaterials, 24, pp. 3247-3253; 
Ylinen, P., Suuronen, R., Taurio, R., Tormala, P., Rokkanen, P., Use of hydroxylapatite/polymer-composite in facial bone augmentation. An experimental study (2002) Int J Oral Maxillofac Surg, 31 (4), pp. 405-409; 
Lu, H.H., El-Amin, S., Scott, K., Laurencin, C., Three-dimensional, bioactive, biodegradable, polymer-bioactive glass composite scaffolds with improved mechanical properties support collagen synthesis and mineralization of human osteoblast-like cells in vitro (2003) J Biomed Mater Res, 64 A, pp. 465-474; 
Navarro, M., Ginebra, M.P., Planell, J.A., Zeppetelli, S., Ambrosio, L., Development and cell response of a new biodegradable composite scaffold for guided bone regeneration (2004) J Mater Sci-Mater Med, 15 (4), pp. 419-422; 
Klein, C.P.A.T., Driessen, A.A., Degroot, K., Vandenhooff, A., Biodegradation behavior of various calcium-phosphate materials in bone tissue (1983) J Biomed Mater Res, 17 (5), pp. 769-784; 
Denissen, H.W., Degroot, K., Makkes, P.C., Vandenhooff, A., Klopper, P.J., Tissue-response to dense apatite implants in rats (1980) J Biomed Mater Res, 14 (6), pp. 713-721; 
Navarro, M., Ginebra, M.P., Clement, J., Martinez, S., Avila, G., Planell, J.A., Physicochemical degradation of titania-stabilized soluble phosphate glasses for medical applications (2003) J Am Ceram Soc, 86 (8), pp. 1345-1352; 
Navarro, M., Ginebra, M.P., Planell, J.A., Cellular response to calcium phosphate glasses with controlled solubility (2003) J Biomed Mater Res Part A, 67 A (3), pp. 1009-1015; 
Li, S.M., Hydrolytic degradation characteristics of aliphatic polyesters derived from lactic and glycolic acids (1999) J Biomed Mater Res, 48 (3), pp. 342-353; 
Burg, K.J.L., Shalaby, S.W., Physicochemical changes in degrading polylactide films (1997) J Biomat Sci Polym, 9 (1), pp. 15-29; 
Li, S.M., Vert, M., Hydrolytic degradation of coral/poly(DL-lactic acid) bioresorbable material (1996) J Biomat Sci-Polym, 7 (9), pp. 817-827; 
Agrawal, C.M., Athanasiou, K.A., Technique to control pH in vicinity of biodegrading PLA-PGA implants (1997) J Biomed Mater Res, 38 (2), pp. 105-114; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., Solutions able to reproduce invivo surface-structure changes in bioactive glass-ceramic A-W3 (1990) J Biomed Mater Res, 24 (6), pp. 721-734; 
Fischer, E.W., Sterzel, H.J., Wegner, G., Investigation of the structure of solution grown crystals of lactide copolymers by means of chemical reactions (1973) Kolloid-Zeitschrift and Z Polymere, 251, pp. 978-990; 
Driessens, F.C.M., Fernandez, E., Ginebra, M.P., Boltong, M.G., Planell, J.A., Calcium phosphates and ceramic bone cements vs. acrylic cements (1997) Anales Quimica, 93 (1), pp. S38-S43; 
Berry, E.E., Baddiel, C.B., Some assignments in infra-red spectrum of octacalcium phosphate (1967) Spectrochim Acta Part A - Mol Spectrosc A, 23 (6), p. 1781; 
Fowler, B.O., Moreno, E.C., Brown, W.E., Infra-red spectra of hydroxyapatite octacalcium phosphate and pyrolysed octacalcium phosphate (1966) Arch Oral Biol, 11 (5), p. 477; 
Elliot, J.C., General chemistry of the calcium orthophosphates. Structure and chemistry of the apatites and other calcium orthophosphates (1994), pp. 1-61. , Elsevier AmsterdamStutman, J.M., Termine, J.D., Posner, A.S., Vibrational spectra and structure of phosphate ion in some calcium phosphates (1965) Trans New York Acad Sci, 27 (6), p. 669; 
Cornilsen, B.C., Condrate, R.A., The vibrational spectra of β-Ca 2P 2 O 7 and γ-Ca 2P 2O 7 (1979) J Inorg Nucl Chem, 41, pp. 602-605; 
Cornilsen, B.C., Condrate, R.A., Vibrational spectra of α-alkaline earth pyrophosphates (1978) J Solid State Chem, 23, pp. 375-382; 
de F{\'a}tima Gimenez, I., Mazali, I.O., Alves, O.L., Application of Raman spectroscopy to the study of the phase composition of phosphate based glass-ceramics (2001) J Phys Chem Solids, 62, pp. 1251-1255; 
Kasuga, T., Abe, Y., Calcium phosphate invert glasses with soda and titania (1999) J Non-Cryst Solids, 243, pp. 70-74; 
Imai, Y., Fukuzawa, A., Watanabe, M., Effect of blending tricalcium phosphate on hydrolytic degradation of a block polyester containing poly(L-lactic acid) segment (1999) J Biomat Sci - Polym, 10 (7), pp. 773-786; 
Huffman, K.R., Casey, D.J., Effect of carboxyl end groups on hydrolysis of polyglycolic acid (1985) J Polym Sci Part A - Polym Chem, 23 (7), pp. 1939-1954; 
Termine, J.D., Eanes, E.D., Comparative chemistry of amorphous and apatitic calcium phosphate preparations (1972) Calcif Tissue Res, 10 (3), p. 171; 
Zahidi, E., Lebugle, A., Bonel, G., On a new class of biomaterials for osseous or dental prosthesis (1985) Bull Soc Chim France, (4), pp. 523-527; 
Eanes, E.D., Gillesse, I.H., Gosner, A.S., Intermediate states in precipitation of hydroxyapatite (1965) Nature, 208 (5008), p. 365; 
Tung, M.S., Brown, W.E., An intermediate state in hydrolysis of amorphous calcium-phosphate (1983) Calcif Tissue Int, 35 (6), pp. 783-790; 
Wuthier, R.E., Rice, G.S., Wallace, J.E.B., Weaver, R.L., Legeros, R.Z., Eanes, E.D., Invitro precipitation of calcium-phosphate under intracellular conditions - Formation of brushite from an amorphous precursor in the absence of Atp (1985) Calcif Tissue Int, 37 (4), pp. 401-410; 
Root, M.J., Inhibition of the amorphous calcium-phosphate phase-transformation reaction by polyphosphates and metal-ions (1990) Calcif Tissue Int, 47 (2), pp. 112-116; 
},
	month = {2005///},
	pages = {411 - 419},
	abstract = {Poly(α-hydroxy acids), and in particular polylactic acid (PLA), are nowadays amongst the most used bioabsorbable materials. However, this polymer may not meet some application requirements due to inadequate mechanical properties and or its degradation characteristics. A possible strategy to tackle this problem is the incorporation of an inorganic phase into the polymeric matrix. In this work a new fully biodegradable composite material made with PLA and calcium phosphate soluble glass particles has been developed. The behaviour of the PLA/ glass composite has been analysed during its degradation in simulated physiological conditions by means of weight loss, molecular weight and thermal properties analysis and electron microscopy observation. The results showed that the incorporation of phosphate glass particles into the polymer significantly accelerated the degradation of the PLA and induced the formation of calcium phosphate precipitates at the composite surface. {\textcopyright} 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Absorbable Implants, Absorption, article, biodegradability, Biodegradable composite, Biomimetic materials, Body fluids, Bone Regeneration, Bone Substitutes, calcium phosphate, Calcium phosphates, composite material, Degradation, electron microscopy, glass, In vitro degradation, in vitro study, Lactic Acid, materials testing, molecular weight, observation, Phosphate glass, polylactic acid, Polymers, precipitation, priority journal, reaction analysis, simulation, Solubility, Surface Properties, surface property, temperature, weight reduction},
	isbn = {17427061 (ISSN)},
	author = {Navarro, M. and Ginebra, M.P. and Planell, J.A. and Barrias, C.C. and Barbosa, M.A.}
}
@conference {2442,
	title = {Neural network classification: Maximizing zero-error density},
	booktitle = {Lecture Notes in Computer ScienceLect. Notes Comput. Sci.},
	series = {Third International Conference on Advances in Patten Recognition, ICAPR 2005},
	volume = {3686},
	year = {2005},
	note = {Conference code: 65875Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Silva, L.M.; Instituto de Engenharia Biom{\'e}dica, Lab. de Sinai e Imagem Biom{\'e}dica, Campus da FEUP, Rua Dr. Roberto Frias, 4200 - 465 Porto, PortugalReferences: Erdogmus, D., Principe, J.C., An error-entropy minimization algorithm for supervised training of nonlinear adaptive systems (2002) IEEE Transactions on Signal Processing, 50 (7), pp. 1780-1786;Erdogmus, D., Principe, J., Generalized information potential criterion for adaptive system training (2002) IEEE Transactions on Neural Networks, 13 (5), pp. 1035~1044; 
Santos, J.M., Alexandre, L.A., Marques De S{\'a}, J., The error entropy minimization algorithm for neural network classification (2004) Int. Conf. on Recent Advances in Soft Computing, , Nottingham, United Kingdom; 
Santos, J.M., Alexandre, L.A., De Sa, J.M., Optimization of the error entropy minimization algorithm for neural network classification (2004) Intelligent Engineering Systems Through Artificial Neural Networks, 14, pp. 81-86. , C. Dagli, A. Buczak, D. Enke, M. Embrechts, and O. Ersoy, editors, ASME Press Series; 
Silva, L.M., De Sa, J.M., Alexandre, L.A., Neural network classification using shannon{\textquoteright}s entropy (2005) European Symposium on Artificial Neural Networks; 
Jacobs, R., Jordan, M., Nowlan, S., Hinton, G., Adaptive mixtures of local experts (1991) Neural Computation, 3, pp. 79-87; 
Blake, C.L., Merz, C.J., (1998) UCI Repository of Machine Learning Databases, , http://www.ics.uci.edu/~mlearn/MLRepository.html, University of California, Irvine, Dept. of Information and Computer Sciences
},
	month = {2005///},
	pages = {127 - 135},
	address = {Bath},
	abstract = {We propose a new cost function for neural network classification: the error density at the origin. This method provides a simple objective function that can be easily plugged in the usual backpropagation algorithm, giving a simple and efficient learning scheme. Experimental work shows the effectiveness and superiority of the proposed method when compared to the usual mean square error criteria in four well known datasets. {\textcopyright} Springer-Verlag Berlin Heidelberg 2005.},
	keywords = {Algorithms, Backpropagation, Backpropagation algorithm, Classification (of information), Costs, Error analysis, Function evaluation, Learning systems, Mean square error, Neural network classification, Neural networks, Zero-error density},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27244450344\&partnerID=40\&md5=73ee3c0c073fb3bc7bc85013bf7dcce6},
	author = {Silva, L.M. and Alexandre, L.A. and De S{\'a}, J.M.},
	editor = {Singh S. and Singh M. and Apte C. and Perner P.}
}
@conference {2446,
	title = {Spectral methods in image segmentation: A combined approach},
	booktitle = {Lecture Notes in Computer ScienceLect. Notes Comput. Sci.},
	series = {Second Iberian Conference on Pattern Recognition and Image Analysis, IbPRIA 2005},
	volume = {3523},
	year = {2005},
	note = {Conference code: 65649Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Monteiro, F.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Campus de Santa Apol{\'o}nia, Apartado 134, 5301-857 Bragan{\c c}a, Portugal; email: monteiro@ipb.ptReferences: Fowlkes, C., Belongie, S., Chung, F., Malik, J., Spectral grouping using nystr{\"o}m method (2004) Trans. on Pattern Analysis and Machine Intelligence, 26 (2), pp. 214-225;Malik, J., Belongie, S., Leung, T., Shi, T., Contour and texture analysis for image segmentation (2001) International Journal of Computer Vision, 43 (1), pp. 7-27; 
Martin, D., Fowlkes, C., Tal, D., Malik, J., A database of human segmented natural images and its application to evaluating segmentation algorithms and measuring ecological statistics (2001) IEEE Int. Conf. on Computer Vision, 2, pp. 416-424; 
Meila, M., Shi, J., Learning segmentation by random walks (2000) Neural Information Processing Systems, pp. 873-879; 
Ng, A., Jordan, M., Weiss, Y., On spectral clustering: Analysis and an algorithm (2002) Advances in Neural Information Processing Systems, 14, pp. 849-856; 
Scott, D.S., Solving sparse symmetric generalized eigenvalue without factorization (1981) SIAM Journal of Numerical Analisys, 18, pp. 102-110. , http://www.netlib.org/laso/snlaso.f; 
Scott, G.L., Longuet-Higgins, H.C., Feature grouping by "relocalisation" of eigenvectors of the proximity matrix (1990) Proc. British Machine Vision Conf., pp. 103-108; 
Shi, J., Malik, J., Normalized cuts and image segmentation (2000) IEEE Trans. on Pattern Analysis and Machine Intelligence, 22 (8), pp. 888-905; 
Weiss, Y., Segmentation using eigenvectors: A unifying view (1999) International Conference on Computer Vision, pp. 975-982; 
Wertheimer, M., Laws of organization in perceptual forms (1938) A Sourcebook of Gestalt Psychology, pp. 71-88. , W. B. Ellis Editor; 
Wu, Z., Leahy, R., An optimal graph theoretic approach to data clustering: Theory and its application to image segmentation (1993) IEEE Trans. on Pattern Analysis and Machine Intelligence, 15, pp. 1101-1113; 
Yu, S., Shi, J., Multiclass spectral clustering (2003) International Conference on Computer Vision, pp. 313-319. , Nice, France
},
	month = {2005///},
	pages = {191 - 198},
	address = {Estoril},
	abstract = {Grouping and segmentation of images remains a challenging problem in computer vision. Recently, a number of authors have demonstrated a good performance on this task using spectral methods that are based on the eigensolution of a similarity matrix. In this paper, we implement a variation of the existing methods that combines aspects from several of the best-known eigenvector segmentation algorithms to produce a discrete optimal solution of the relaxed continuous eigensolution. {\textcopyright} Springer-Verlag Berlin Heidelberg 2005.},
	keywords = {Algorithms, Computer vision, Discrete optimal solution, Eigensolution, Eigenvalues and eigenfunctions, Image segmentation, Matrix algebra, Problem solving, Similarity matrix, Spectral methods},
	isbn = {03029743 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-25144437374\&partnerID=40\&md5=5b6351af4ac58a16c41b5558a48ae9e2},
	author = {Monteiro, F.C. and Campilho, A.C.},
	editor = {Marques J.S. and Perez de la Blanca N. and Pina P.}
}
@book {2486,
	title = {CaO-P                    2O
                    5 Glass-Hydroxyapatite Thin Films Obtained by Laser Ablation: Characterisation and In Vitro Bioactivity Evaluation
},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: mpferraz@ufp.ptReferences: Arias, J.L., (1997) J Mater. Sci. Mater. Med., 8, pp. 873-876;Peraire, C., (1997) 13 th European Conference on Biomaterials, p. 75. , G{\"o}teborg, Sweden, 4-7 Sept; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., Fernandes, M.H., Trigo-Cabral, A., Santos, J.D., Monteiro, F.J., (1999) J. Mat Sci: Mat. Med, 10 (9), p. 567
},
	month = {2004///},
	pages = {347 - 350},
	address = {Porto},
	abstract = {Hydroxyapatite (HA) coatings have been applied to improve adhesion of non-cemented implants to host bone. Plasma spraying is the most common technique leading to thick calcium phosphate films (\&gt;120μm). Pulse laser deposition (PLD), is a possible alternative method to obtain thin (\&lt;10 μm), well adherent hydroxyapatite (HA) films. Similarly to synthetic HA, biological apatites contain Ca                        2+, PO
                        4
                        3- and OH
},
	keywords = {2O, 5 glass, Adhesion, Bioactivity, Bone, Calcium Compounds, CaO-P, Coatings, Deposition, Energy dispersive spectroscopy, Homogeneous mixtures, Hydroxylapatite thin films, Implants (surgical), In vitro bioactivity, Laser ablation, Plasma spraying, Scanning electron microscopy, Thin films, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516195\&partnerID=40\&md5=ddd47b73956669a35aa9041ce9102612},
	author = {Ferraz, M.P. and Monteiro, F.J. and Gi{\~a}o, D. and Leon, B. and Gonzalez, P. and Liste, S. and Serra, J. and Arias, J. and Perez-Amor, M.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@conference {2470,
	title = {"In vitro" bioactivity evaluation of CaO-P                    2O 
                    5 glass-hydroxylapatite thin coatings produced by pulsed laser deposition
},
	booktitle = {Transactions - 7th World Biomaterials CongressTrans. World Biomater. Congr.},
	series = {Transactions - 7th World Biomaterials Congress},
	year = {2004},
	note = {Conference code: 64310Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Arias, J.L.; Depto. de F{\'\i}sica Aplicada, Universidade de Vigo, Lagoas-Marcosende 9, E-36200 Vigo, SpainReferences: Arias, J.L., (1997) J Mater Sci Mater Med, 8, p. 873;Ferraz, M.P., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., (1999) J. Mat Sci Mat Med, 10, p. 567
},
	month = {2004///},
	pages = {1774},
	address = {Sydney},
	abstract = {The synthesis of coatings based on hydroxyapatites (HA) and glass modified hydroxyapatite targets by pulsed laser deposition, was presented. It was observed that coatings produced from HA targets keep their crystallinity through all their thickness, while there is a loss of crystallinity towards the surface of the HA+1.5G coatings. On immersion in SBF for three days, the coatings produced from HA targets suffered a loss of crystallinity near the surfaces, while the crystallinity of the coatings produced from HA+1.5G targets increased. It was also observed that addition of 1.5wt\% of CaO-P                        2O
                        5 glasses to HA thin coatings produced by pulsed laser deposition leads to a significant structural change of the coating surface.
},
	keywords = {Apatites, Bioactivity, Calcium Compounds, Coatings, Crystalline materials, Crystalline phases, hydroxyapatite, Mixtures, Phosphorus Compounds, Pressure effects, Pulsed laser deposition, Scanning electron microscopy, Substrates, Thin coatings, X ray diffraction, X ray photoelectron spectroscopy},
	isbn = {1877040193 (ISBN); 9781877040191 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-13844299568\&partnerID=40\&md5=869dc43fe34d9345a4c3b944a06faad1},
	author = {Arias, J.L. and Liste, S. and Gonz{\'a}lez, P. and Serra, J. and Le{\'o}n, B. and Ferraz, M.P. and Monteiro, F.J. and Giao, D. and P{\'e}rez-Amor, M.}
}
@book {2496,
	title = {Study of a Silicate Cement for Dental Applications},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Pereira, S.; Fac. de Medicina Dent{\'a}ria, Universidade do Porto, R. Dr. Manuel Pereira da Silva, 4200-393 Porto, PortugalReferences: Craig, R.G., Rovers, J.M., (2002) Restorative Dental Materials. 9th Ed., pp. 593-627. , St Louis: Mosby;Gladwin, M., Bagby, M., (2000) Clinical Aspects of Dental Materials. First Edition, pp. 81-92. , Philadelphia: Lippincott Williams \& Wilkins; 
Leinfelder, K.F., Changing Restorative Traditions: The Use of Bases and Liners (1994) JADA, 125, pp. 65-67; 
Macchi, R.L., (2000) Mat. Dentales. 3 a Ed., pp. 129-135. , Buenos Aires: Edit. M{\'e}dica P.americana S.A; 
Wise, D.L., (1995) Encyclopedic Handbook of Biomaterials and Bioengineering, pp. 1398-1411. , New York: Marcel Dekver; 
Gordan, V.V., Mj{\"o}r, I.A., Hucke, R.D., Smith, G.E., Effect of Different Liner Treatments on Postoperative Sensivity of Amalgam Restorations (1999) Quintessence Int, 30, pp. 55-59; 
Me, M., Nakai, H., Effect of Immersion in Water on Linear Expansion and Strength of Three Base/Liner Materials (1995) Dent Mater, 14, pp. 70-77; 
Leevailoj, C., Cochran, M.A., Matis, B.A., Moore, B.K., Platt, J.A., Microleakage of Posterior Packable Resin Composites with and without Flowable Liners (2001) Oper Dent, 26, pp. 302-307; 
Weiner, R.S., Weiner, L.K., Kugel, G., Teaching the Use of Bases and Liners: A Survey of the North American Dental Schools (1996) JADA, 127, pp. 1640-1645; 
Pereira, S., Branco, R., Cavalheiro, J., Afonso, A., Vasconcelos, M., (2003) Sealing Ability of a New Dental Base Material, , Bioeng; 
(1991) Specification for Dental Water-based Cements, , ISO 9917 (F)
},
	month = {2004///},
	pages = {659 - 662},
	address = {Porto},
	abstract = {Dental cements have a wide field of applications in clinical practice. The use as a base material is a very important one in dentistry. The aim of this study is to assess some relevant properties of a silicate cement (SC) developed for dental applications, comparing it with two other cements already commercialised, a zinc phosphate cement De Trey Zinc Cement Improved{\textregistered} (DT) and ProRootMTA{\textregistered}(MTA). Their compressive strength, dimensional changes and weight variation in water after setting, according to ISO standards were compared. The mean compressive strength of DT, MTA and SC were, respectively, 134MPa, 31MPa and 46MPa. There were no statistically significant differences for the three tested cements at the various time intervals, in respect to their weight variation or dimensional changes. However, there was a little loss of weight for DT (0.15\%) and for MTA (3\%) and a gain of 3\% for the SC. Regarding the dimensional changes, all materials undergo little expansion, more significant in the MTA and in the SC samples of 2,07\% and 0,4\%, respectively. The new Silicate Cement, presented enough compressive strength, low weight variation, very small dimensional changes and good adaptation to the surface of dental cavity. For these reasons this new cement could be a valuable proposal as a base material for dental applications.},
	keywords = {Biocompatibility, Body fluids, Ceramic materials, ceramics, Compressive Strength, Dental cement, Dental cements, Dental prostheses, Dimensional stability, Precipitation (chemical), Scanning electron microscopy, Silicate cements, Silicates, Stainless steel, Weight variation},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346255102\&partnerID=40\&md5=b43c6052d3f4c9fcaff59b75a9cad543},
	author = {Pereira, S. and Cavalheiro, J. and Branco, R. and Afonso, A. and Vasconcelos, M.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2480,
	title = {Ultrastructural comparison of dissolution and apatite precipitation on hydroxyapatite and silicon-substituted hydroxyapatite in vitro and in vivo},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {69},
	year = {2004},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: JBMRCPubMed ID: 15162409Language of Original Document: EnglishCorrespondence Address: Porter, A.E.; Dept. of Mat. Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge, CB2 3QZ, United Kingdom; email: aep30@cam.ac.ukChemicals/CAS: apatite, 64476-38-6; carbonic acid, 3812-32-6, 463-79-6; hydroxyapatite, 1306-06-5, 51198-94-8; silicon, 7440-21-3; Biocompatible Materials; Durapatite, 1306-06-5; Silicon, 7440-21-3References: Porter, A.E., Best, S.M., Bonfield, W., Ultrastructural comparison of hydroxyapatite and silicon-substituted hydroxyapatite for biomedical applications (2003) J Biomed Mater Res, 68 A, pp. 133-141;Patel, N., Gibson, I.R., Hing, K.A., Best, S.M., Revell, P.A., Bonfield, W., A comparative study on the in vivo behaviour of hydroxyapatite and silicon substituted hydroxyapatite granules (2002) J Mater Sci Mater Med, 13, pp. 1199-1206; 
Porter, A.E., Patel, N., Skepper, J.N.S., Best, S.M., Bonfield, W., Comparison of in vivo dissolution processes in hydroxyapatite and silicon-hydroxyapatite bioceramics (2003) Biomaterials, 24, pp. 4609-4620; 
Weng, J., Liu, Q., Wolke, J.G.C., Zhang, X., De Groot, K., Formation and characteristics of the apatite layer on plasma-sprayed hydroxyapatite coatings in simulated body fluid (1995) Biomaterials, 18, pp. 1027-1035; 
Doi, Y., Shibutani, T., Moriwake, Y., Kajimoto, T., Iwayama, Y., Sintered carbonate apatites as bioresorbable bone substitutes (1997) J Biomed Mater Res, 39, pp. 603-610; 
Schepers, E., Declercq, M., Ducheyne, P., Kempeneers, R., Bioactive glass particulate material as a filler for bone lesions (1991) J Oral Rehab, 18, pp. 439-452; 
Ducheyne, P., Qiu, Q., Bioactive ceramics: The effect of surface reactivity on bone formation and bone cell function (1999) Biomaterials, 20, pp. 2287-2303; 
Ducheyne, P., Radin, S., King, L., The effect of calcium-phosphate ceramic composition and structure on in vitro behaviour. I. Dissolution (1993) J Biomed Mater Res, 27, pp. 25-34; 
Kukubo, T., Kushitani, H., Sakka, S., Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 721-734; 
Radin, S.R., Ducheyne, P., The effect of calcium phosphate ceramic composition and structure on in vitro behavior. II. Precipitation (1993) J Biomed Mater Res, 27, pp. 35-45; 
Gibson, I.R., Hing, K.A., Best, S.M., Bonfield, W., Enhanced in vitro cell activity and surface apatite layer formation on novel silicon-substituted hydroxyapatites (1999) 12th International Symposium on Ceramics in Medicine, pp. 191-194. , Ohgushi H, Hastings GW, Yoshikawa T, editors. Nara, Japan. World Scientific; 
Fujibayashi, S., Neo, M., Kim, H.-M., Kokubo, T., Nakamura, T., A comparative study between in vivo bone ingrowth and in vitro apatite formation on Na 2-CaO-SiO 2 glasses (2003) Biomaterials, 24, pp. 1349-1356; 
Porter, A.E., Patel, N., Skepper, J.N., Best, S.M., Bonfield, W., Effect of sintered silicate-substituted hydroxyapatite on remodelling processes at the bone-implant interface (2003) Biomaterials, 25, pp. 3303-3314; 
Leng, Y., Chen, J., Qu, S., TEM study of calcium phosphate precipitation on HA/TCP ceramics (2003) Biomaterials, 24, pp. 2125-2131; 
Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 28, p. 809; 
Gibson, I.R., Best, S.M., Bonfield, W., Chemical characterization of silicon-substituted hydroxyapatite (1999) J Biomed Mater Res, 44, pp. 422-428; 
Rey, C., Calcium phosphates for medical applications (1998) Calcium Phosphates in Biological and Industrial Systems, pp. 217-251. , Amjad Z, editor. Kluwer Academic: Boston; 
Tanizawa, Y., Suzuki, T., Effects of silica ions on the formation and transformation of calcium phosphates in neutral aqueous solutions (1995) J Chem Soc Faraday Trans, 91, pp. 3499-3503; 
Damen, J.J.M., Ten Cate, J.M., Silica-induced precipitation of calcium phosphate in the presence of inhibitors of hydroxyapatite formation (1992) J Dent Res, 71, pp. 453-457; 
Botelho, C.M., Lopes, M.A., Gibson, I.R., Best, S.M., Santos, J.D., Structural analysis of Si-substituted hydroxyapatite: Zeta potential and X-ray photoelectron spectroscopy (XPS) (2002) J Mater Sci Mater Med, 13, pp. 1123-1127; 
Carlisle, E.M., Silicon (1975) Nutr Rev, 33, pp. 257-260; 
Termine, J.D., Eanes, E.D., Conn, K.M., Phosphoprotein modulation of apatite crystallization (1980) Calcif Tissue Int, 31, pp. 247-251; 
Combes, C., Rey, C., Adsorption of proteins and calcium phosphate materials bioactivity (2002) Biomaterials, 23, pp. 2817-2823; 
Horbett, T.A., Proteins at interfaces - An overview (1995) ACS Symp Ser, pp. 602-623
},
	month = {2004///},
	pages = {670 - 679},
	abstract = {Recent histological studies have demonstrated that the substitution of silicate ions into hydroxyapatite (HA) significantly increases the rate of bone apposition to HA implants. The enhanced bioactivity of silicon-substituted HA (Si-HA) over pure HA has been attributed to the effect of silicate ions in accelerating dissolution. In the present study, high-resolution transmission electron microscopy (HR-TEM) was employed to compare dissolution of HA and Si-HA in an acellular simulated body fluid (SBF) to dissolution in an in vivo model. HR-TEM observations confirmed a difference in morphology of apatite precipitates in vivo and in SBF: apatite deposits were platelike in vivo and nodular in SBF. Compositional mapping suggested that preferential dissolution of silicon from the implant promotes the nucleation of carbonate apatite around the implant. The in vivo findings illustrated an absence of dissolution at the bone-HA or Si-HA interface, whereas dissolution was extensive from within the implant. The amount of dissolution in acellular SBF was similar to dissolution from within the implant, although the site at which the dissolution nucleates was different: dissolution predominates at the crystallite surfaces in SBF, whereas grain boundary dissolution predominates in vivo. These findings suggest that proteins in the in vivo milieu modify the processes of dissolution from the implant. {\textcopyright} 2004 Wiley Periodicals, Inc.},
	keywords = {Apatite, article, Bioactivity, Biocompatible Materials, body fluid, carbonic acid, comparative study, Composition, Dissolution, Durapatite, histology, hydroxyapatite, implant, Implants (surgical), in vitro study, in vivo study, Interfaces (materials), Microscopy, Electron, Microscopy, Electron, Scanning, morphology, precipitation, Precipitation (chemical), Silicon, Simulated body fluid, Simulated body fluids (SBF), simulation, transmission electron microscopy, ultrastructure},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2942558665\&partnerID=40\&md5=c262d4d218d8dba93d767c3b6f399564},
	author = {Porter, A.E. and Botelho, C.M. and Lopes, M.A. and Santos, J.D. and Best, S.M. and Bonfield, W.}
}
@article {2521,
	title = {Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot particles for dental implant applications},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 77Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(02)00314-9Language of Original Document: EnglishCorrespondence Address: Aparicio, C.; Res. Center in Biomed. Eng. (CREB), Department of Materials Science, Universitat Politecnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, Spain; email: conrado.aparicio@upc.esChemicals/CAS: Dental Implants; Titanium, 7440-32-6References: Br{\r a}nemark, P.I., Hansson, B.O., Adell, R., Breine, U., Lindstr{\"o}m, J., Hallen, O., {\"O}hman, A., Osseointegrated implants in the treatment of edentulous jaw experience from a 10-year period (1977) Scand J Reconstr Surg, 16 (SUPPL.), pp. 1-132;Lemons, J.E., Application of materials in medicine and dentistry. Dental implants (1996) Biomaterials Science: An Introduction to Materials in Medicine, pp. 308-318. , B.D. Ratner, A.S. Hoffman, F.J. Schoen, \& J.E. Lemons. San Diego: Academic Press; 
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},
	month = {2003///},
	pages = {263 - 273},
	abstract = {It is well known that the osseointegration of the commercially pure titanium (c.p. Ti) dental implant is improved when the metal is shot blasted in order to increase its surface roughness. This roughness is colonised by bone, which improves implant fixation. However, shot blasting also changes the chemical composition of the implant surface because some shot particles remain adhered on the metal. The c.p. Ti surfaces shot blasted with different materials and sizes of shot particles were tested in order to determine their topographical features (surface roughness, real surface area and the percentage of surface covered by the adhered shot particles) and electrochemical behaviour (open circuit potential, electrochemical impedance spectroscopy and cyclic polarisation). The results demonstrate that the increased surface area of the material because of the increasing surface roughness is not the only cause for differences found in the electrochemical behaviour and corrosion resistance of the blasted c.p. Ti. Among other possible causes, those differences may be attributed to the compressive residual surface stresses induced by shot blasting. All the materials tested have an adequate corrosion and electrochemical behaviour in terms of its possible use as dental implant material. {\textcopyright} 2002 Elsevier Science Ltd. All rights reserved.},
	keywords = {article, Blast finishing, chemical composition, chemistry, Corrosion, Dental implant, Dental Implants, Dental prostheses, Electric Potential, Electrochemical impedance spectroscopy, electrochemistry, impedance, materials testing, Particle Size, Polarization, priority journal, Shot blasting, Spectroscopy, stress, Surface roughness, Surface topography, Titanium, tooth implantation},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037209960\&partnerID=40\&md5=c60c6b69f4fb345454b557acc836ec95},
	author = {Aparicio, C. and Javier Gil, F. and Fonseca, C. and Barbosa, M. and Planell, J.A.}
}
@article {2519,
	title = {Dissolution studies of hydroxyapatite and glass-reinforced hydroxyapatite ceramics},
	journal = {Materials CharacterizationMater Charact},
	volume = {50},
	year = {2003},
	note = {Conference code: 61615Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: MACHEdoi: 10.1016/S1044-5803(03)00092-5Language of Original Document: EnglishCorrespondence Address: Prado da Silva, M.H.; DEMP, UFC, Campus PICI, Bl. 714, Fortaleza, CE 60455-760, Brazil; email: mhprado@dem.ufc.brReferences: Barinov, S.M., Baschenko, Y.V., Application of ceramic composites as implants: Result and problem (1992) Bioceramics and the Human Body, pp. 206-210. , A. Ravaglioli, \& A. Krajewski. United Kingdom: Elsevier;Nishihara, K., Studies on peri-root tissue formation around new type artificial root made of dense hydroxyapatite (1993) Clin. Mater., 12, pp. 159-167; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J. Biomed. Mater. Res., 45, pp. 370-375; 
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},
	month = {2003///},
	pages = {197 - 202},
	address = {Rio de Janeiro},
	abstract = {In the continuous agitation assays, glass-reinforced hydroxyapatite (GR-HA) was shown to form a calcium phosphate (CaP) layer, but hydroxyapatite (HA) only formed dispersed precipitates. The formation of this layer was first detected on the GR-HA with a 7.5\% glass addition (7.5 GR-HA) after only 3 days of immersion in simulated body fluid (SBF). The time required for layer formation decreased as the amount of glass added to the HA increased. The dissolution rate of the materials followed a similar pattern, i.e. the dissolution rate for GR-HA was higher than for HA, and increased with the addition of glass. The immersion of 7.5 GR-HA in water showed almost linear dissolution kinetics over the immersion periods (3, 7, 15, 30 and 60 days). The concentration of calcium ions in solution and the scanning electron microscopy (SEM) analysis of the 7.5 GR-HA specimens immersed in water and in SBF revealed a clear competition between the material dissolution and the precipitation of a CaP phase. Fourier transformed infrared spectroscopy with alternated total reflectance (FTIR-ATR) analysis indicated that the CaP phase that formed during longer immersion times (30 and 60 days) could be a carbonate-substituted CaP precipitate. As expected from previous work, the GR-HA behavior in terms of its in vitro bioactivity is higher than HA because a homogeneous CaP layer is formed and the precipitation occurs faster. From the dissolution test and in accordance with the chemical composition of the samples, GR-HA was more soluble than HA. {\textcopyright} 2003 Elsevier Inc. All rights reserved.},
	keywords = {Ceramic materials, Dissolution, glass, Glass-reinforced hydroxyapatite, hydroxyapatite, Immersion times, Scanning electron microscopy},
	isbn = {10445803 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0141895021\&partnerID=40\&md5=9226464f75e2ecd20d23f9d42e61d69e},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J. and Prado da Silva, M.H.},
	editor = {Solorzano G. and Freire Jr. F.L. and Soares G.A. and Souza P.L.}
}
@article {2531,
	title = {Porous glass reinforced hydroxyapatite materials produced with different organic additives},
	journal = {Journal of Non-Crystalline SolidsJ Non Cryst Solids},
	volume = {304},
	year = {2002},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: JNCSBdoi: 10.1016/S0022-3093(02)01036-0Language of Original Document: EnglishCorrespondence Address: Prado da Silva, M.H.; DEM, Universidade Federal do Ceara, PO Box 12144, 60455-760 Fortaleza, CE, Brazil; email: mpsilva@ibmc.up.ptReferences: Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., (2000) Biomaterials, 21, p. 813;Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res., 47, p. 603; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res., 45, p. 376; 
Silva, P.L., Santos, J.D., Monteiro, F.J., Knowles, J.C., (1998) Surf. Coat. Tech., 102, p. 191; 
Zhang, Y., Santos, J.D., (2000) J. Non-Cryst. Solids, 272, p. 14; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mater. Res., 54, p. 463; 
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Knowles, J.C., Santos, J.D., Hastings, G.W., patent number WO0068164, 2000Larson, A.C., Von Dreele, R.B., Lujan M., Jr., GSAS, Neutron Scattering Centre, Los Alamos National Laboratory, CA, 1990Kay, M.I., Young, R.A., Posner, A.S., (1964) Nature, 204, p. 1050; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., (1998) J. Biomed. Mater. Res., 39, p. 244
},
	month = {2002///},
	pages = {286 - 292},
	abstract = {In the present study three different organic additives were used to produce porous structures of a CaO-P                        2O
                        5 glass reinforced hydroxyapatite potato starch, almond crust and wax spheres. The produced samples were analysed by scanning electron microscopy, X-ray diffraction with Rietveld refinement, differential thermal analysis and mercury porosimetry. The techniques used in this study enabled the production of glass reinforced hydroxyapatite samples with various pore diameters. Two different techniques were used to produce porous glass reinforced hydroxyapatite samples: a dry method using wax spheres as pore formers and a wet method in alcoholic suspension, where almond crust and potato starch were used as pore formers. The final microstructure consists of hydroxyapatite, α-tricalcium phosphate and β-tricalcium phosphate. X-ray diffraction and scanning electron microscopy analysis revealed different percentages of phases when comparing dense and porous glass reinforced hydroxyapatite specimens. These hard materials are intended to be used as bone defect fillers. {\textcopyright} 2002 Elsevier Science B.V. All rights reserved.
},
	keywords = {Additives, Differential thermal analysis, Fillers, glass, hydroxyapatite, Microstructure, Porosimeters, Porous glasses, Porous materials, Scanning electron microscopy, Suspensions (fluids), X ray diffraction analysis},
	isbn = {00223093 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036605142\&partnerID=40\&md5=fe083da9c9790ba4439e741dc6eb8760},
	author = {Prado da Silva, M.H. and Lemos, A.F. and Gibson, I.R. and Ferreira, J.M.F. and Santos, J.D.}
}
@book {2522,
	title = {Production of porous biomaterials based on glass-reinforced hydroxyapatite composites},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {230-232},
	year = {2002},
	note = {Conference code: 60281Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Prado da Silva, M.H.; Instituto de Engenharia Biomedica, Laborat{\'o}rio de Biomaterials, Rua do Campo Alegre 823, PT-4150-180 Porto, PortugalReferences: Lemos, A.F., Ferreira, J.M.F., (2000) Materials Science and Engineering, 11, pp. 35-40;Saggio-Woyansky, J., Scott, C.E., Minnear, W.P., (1992) Amer. Cer. Society Bulletin, 71 (11), pp. 1674-1682; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inouue, H., (2001) J. Biom. Mater. Res., 54, pp. 463-469; 
Knowles, J.C., Santos, J.D., Hastings, G.W., (2000) Sintered Hydroxyapatite Composition and Method for the Preparation thereof, , Patent number WO0068164
},
	month = {2002///},
	pages = {483 - 486},
	address = {Coimbra},
	abstract = {In this study, porous GR-HA composites were produced using a calcium-phosphate glass with the composition, in mol\%, 75P                        2O
                        5-15CaO-10CaF
                        2. The composites were obtained by wet mixing 4.0\% (w/w) of the glasses with hydroxyapatite (HA) powder (batch P120) (Plasma Biotal; Tideswell, U.K.), pressing and sintering. Two different organic additives were used as pore formers: potato starch and almond crust. This processing route was effective in producing a homogeneous microstructure of equiaxed grains. The bulk density of the samples decreased with the volume fraction increase in the content of pore formers. Scanning electron microscopy and mercury porosimetry analyses showed to be complementary techniques in characterising the pore size and distribution.
},
	keywords = {Biomaterials, Composite materials, glass, Glass-reinforced hydroxyapatite composites, GR-HA Composites, hydroxyapatite, Macroporous Bioceramics, Mercury (metal), Microstructure, Organic Additives, Pore size, Porous materials, Powders, Pressing (forming), Scanning electron microscopy, Sintering, Starch},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036432238\&partnerID=40\&md5=9af5590c70f4defe5e87b9a72042e11b},
	author = {Prado da Silva, M.H. and Lemos, A.F. and Ferreira, J.M.F. and Lopes, M.A. and Santos, J.D.},
	editor = {Vieira T.}
}
@article {2527,
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	year = {2002},
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},
	month = {2002///},
	pages = {4994 - 5003},
	abstract = {Nacre organic matrix has been conventionally classified as both {\textquoteright}water-soluble{\textquoteright} and {\textquoteright}water-insoluble{\textquoteright}, based on its solubility in aqueous solutions after decalcification with acid or EDTA. Some characteristics (aspartic acid-rich, silk-fibroin-like content) were specifically attributed to either one or the other. The comparative study on the technique of extraction (extraction with water alone vs. demineralization with EDTA) presented here, seems to reveal that this generally accepted classification may need to be reconsidered. Actually, the nondecalcified soluble organic matrix, extracted in ultra-pure water, displays many of the characteristics of what until now has been called {\textquoteright}insoluble matrix{\textquoteright}. We present the results obtained on this extract and on a conventional EDTA-soluble matrix, with various characterization methods: fractionation by size-exclusion and anion-exchange HPLC, amino acid analysis, glycosaminoglycan and calcium quantification, SDS/PAGE and FTIR spectroscopy. We propose that the model for the interlamellar matrix sheets of nacre given by Nakahara [In: Biomineralization and Biological Metal Accumulation, Westbroek, P. \& deJong, E.W., eds, (1983) pp. 225-230. Reidel, Dordrecht, Holland] and Weiner and Traub [Phil. Trans. R. Soc. Lond. B (1984) 304, 425-434] may no longer be valid. The most recent model, proposed by Levi-Kalisman et al. [J. Struct. Biol. (2001) 135, 8-17], seemed to be more in accordance with our findings.},
	keywords = {amino acid analysis, Amino acids, Animals, anion exchange chromatography, article, Biochemistry, biomineralization, bivalve, Bivalvia, Calcium, Chemical Fractionation, Chromatography, High Pressure Liquid, controlled study, demineralization, edetic acid, EDTA-soluble matrix, Electrophoresis, Polyacrylamide Gel, Extracellular Matrix Proteins, extraction, fractionation, gel permeation chromatography, glycosaminoglycan, Glycosaminoglycans, High performance liquid chromatography, hydrophobicity, infrared spectroscopy, Insect Proteins, model, Nacre, nonhuman, Ostreidae, Pinctada, Pinctada maxima, polyacrylamide gel electrophoresis, priority journal, Silk, Silk-fibroin-like-proteins, Solubility, Spectroscopy, Fourier Transform Infrared, Undecalcified soluble matrix, Water},
	isbn = {00142956 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036408881\&partnerID=40\&md5=7e99def46be711f02de5f4dd3dc8f63e},
	author = {Pereira-Mouri{\`e}s, L. and Almeida, M.-J. and Ribeiro, C. and Peduzzi, J. and Barth{\'e}lemy, M. and Milet, C. and Lopez, E.}
}
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},
	month = {2001///},
	pages = {3341 - 3353},
	abstract = {The present study concerns the investigation of a material expected to be biocompatible and able to promote bone regeneration. For this purpose, cellulose was chemically modified by phosphorylation. Once implanted, phosphorylated cellulose could promote the formation of calcium phosphates, thus having closer resemblance to bone functionality. In a previous investigation, the obtention and the preliminary characterization of cellulose phosphate gels were reported. In the present study, the synthesis by the H                        3PO
                        4/P
                        2O
                        5/Et
                        3PO 
                        4/hexanol method was optimized in terms of reaction parameters. The structure of materials was investigated by FTIR, Raman, and solid-state 
                        31P- and 
                        13C-NMR spectroscopic studies, and X-ray diffraction. Water swelling and stability to sterilization by gamma-radiation were also assessed. It was demonstrated that the present method allows highly phosphorylated cellulose derivatives to be obtained. Cellulose triphosphates gels are described here for the first time. Products obtained were poorly crystallized monoesters, apparently not significantly affected by gamma sterilization and showed a high water swelling capability. Chemical bonding was confirmed by FTIR, Raman, and both 
                        31P- and 
                        13C-NMR spectroscopies. It was also shown that the H
                        3PO
                        4/P
                        2O
                        5/Et
                        3PO 
                        4/hexanol method provides a versatile and interesting alternative route to some of the more widely used techniques for the phosphorylation of cellulose. {\textcopyright} 2001 John Wiley \&amp; Sons, Inc. J. Appl. Polym. Sci.
},
	keywords = {13C-NMR, 2O, 3PO, 4/hexanol method, 4/P, 5/Et, Biomaterials, Cellulose derivatives, Cellulose gels, Cellulose triphosphates, Chemical bonds, Fourier transform infrared spectroscopy, Gels, H, medical textile, Nuclear magnetic resonance spectroscopy, Phosphorylation, Raman scattering, Sterilizers, Synthesis (chemical), X ray diffraction analysis},
	isbn = {00218995 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035923986\&partnerID=40\&md5=7a58f84a4d86e26d35d7d90d44aecb63},
	author = {Granja, P.L. and Pouysgu, L. and Ptraud, M. and De Jso, B. and Baquey, C. and Barbosa, M.A.}
}
@article {2537,
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	volume = {82},
	year = {2001},
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},
	month = {2001///},
	pages = {3354 - 3365},
	abstract = {Cellulose regenerated by the viscose process was previously investigated as an implantable material in orthopedic surgery. It was envisaged to take advantage not only of its good matching with mechanical properties of bone but also of its hydroexpansivity, therefore allowing a satisfactory fixation to hard tissue. Both the osteoconduction and the lack of ostcoinduction of this material were demonstrated. Grafting of phosphate groups was then envisaged as the means to render cellulose more suitable for orthopedic applications by enhancing its bioactivity. In the present work, the previously optimized phosphorylation reaction was successfully adapted to the surface modification of regenerated cellulose. Modified materials were characterized by XPS, FTIR, and                        31P MAS NMR spectroscopic studies, and contact angle measurements, revealing the chemical bond between phosphate groups and cellulose, as well as the hydrophilic nature of phosphorylated materials, which increases with increasing phosphate contents. Water swelling and resistance to gamma sterilization were assessed as well, showing that phosphorylated materials swell considerably in water and were not affected when sterilization was carried out under a nitrogen atmosphere. The increase in surface roughness attributed to chemical modification was demonstrated through laser rugosimetry measurements. {\textcopyright} 2001 John Wiley \&amp; Sons, Inc. J. Appl. Polym. Sci.
},
	keywords = {2O, 3PO, 4/hexanol method, 4/P, 5/Et, Biomaterials, Cellulose derivatives, Cellulose phosphates, Chemical bonds, Chemical modification, Energy dispersive spectroscopy, Fourier transform infrared spectroscopy, H, Hydrogels, Phosphorylation, Regenerated cellulose hydrogels, Sterilization (cleaning), Surface modification, Surface roughness, X ray photoelectron spectroscopy},
	isbn = {00218995 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035923980\&partnerID=40\&md5=5663bc99f91ce47534779e826f87ed97},
	author = {Granja, P.L. and Pouysgu, L. and Ptraud, M. and De Jso, B. and Baquey, C. and Barbosa, M.A.}
}
@article {2540,
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},
	month = {2001///},
	pages = {306 - 312},
	abstract = {In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 μg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. {\textcopyright} 2001 John Wiley \& Sons, Inc.},
	keywords = {alkaline phosphatase, Animals, article, Biomedical engineering, BMP-2, Bone, bone development, bone marrow, Bone Marrow Cells, Bone morphogenetic proteins, Cell culture, Cell Line, cell proliferation, Cells, concentration response, dexamethasone, differentiation inducing factor, dose response, Dose-Response Relationship, Drug, enzyme activity, fibroblast, Fibroblasts, Glucocorticoids, human, human cell, Humans, male, MRC-5 fibroblasts, Nacre, natural product, osteoblast, Ostreidae, oyster, phenotype, Proteins, Pulmonary diseases, Rats, Rats, Wistar, Solubility, stroma cell, time, Time Factors, Transforming Growth Factor beta, unclassified drug, Water, Water-soluble matrix},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034769134\&partnerID=40\&md5=f0ec664a074b3a67745c806152793d54},
	author = {Almeida, M.J. and Pereira, L. and Milet, C. and Haigle, J. and Barbosa, M. and Lopez, E.}
}
@article {2565,
	title = {Brazing parameters determine the degradation and mechanical behaviour of alumina/titanium brazed joints},
	journal = {Journal of Materials ScienceJ Mater Sci},
	volume = {35},
	year = {2000},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: JMTSAdoi: 10.1023/A:1004776117823Language of Original Document: EnglishCorrespondence Address: Paiva, O.C.; Inst de Engenharia Biomedica, Porto, PortugalReferences: Samandi, M., Gudze, M., Evans, P., Nucl, (1997) Instr. and Meth. in Phys. Res. B, 127-128, p. 669;Paiva, O.C., Barbosa, M.A., (1997) J. Mater. Sci., 32, p. 653; 
Moret, F., Eustathopoulos, N., (1993) J. Physique IV, 3, p. 1043. , Colloque C7; 
Suganuma, K., Okamoto, T., Koizumi, M., Shimada, M., (1985) J. Amer. Ceram. Soc., 68, pp. C334; 
Cao, H.C., Thouless, M.D., Evans, A.G., (1988) Acta Metall. Matter., 36, p. 2037; 
Hao, H., Wang, Y., Jin, Z., Wang, X., (1995) J. Amer. Ceram. Soc., 78, p. 2157; 
Yamada, T., Yokoi, K., Kohno, J., (1990) J. Mater. Sci., 25, p. 2188; 
Elssener, G., (1990) Joining of Ceramics, p. 128. , edited by M. G. Nicholas Chapman and Hall, London; 
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Suganuma, K., Okamoto, T., Koizumi, M., Shimada, M., (1985) J. Mater. Sci., 20, p. 648; 
Xian, A.P., Si, Z.Y., (1992) J. Mater. Sci., 27, p. 1560; 
Locatelli, M.R., Dalgleish, B.J., Nakashima, K., Tomsia, A.P., Glaeser, A.M., (1997) Ceramics International, 23, p. 313; 
Suganuma, K., (1990) Joining of Ceramics, p. 173. , edited by M. G. Nicholas Chapman and Hall, London; 
Murakawa, H., Ueda, Y., (1989) Trans. JWRI, 18, p. 295; 
Zhai, Y., North, T.H., Ren, J., (1997) J. Mater. Sci., 32, p. 1399; 
Nicholas, M.G., (1990) Joining of Ceramics, p. 73. , edited by M. G. Nicholas Chapman and Hall, London; 
Peteves, S.D., Ceccone, G., Paulasto, M., Stamos, V., Yvon, P., (1996) JOM, 48, p. 48; 
Li, J.G., (1994) Ceramics International, 20, p. 391; 
Loehman, R.E., Tomsia, A.P., (1992) Acta Metall. Mater., 40 (SUPPL.), pp. S75; 
Schwartz, M.M., (1990) Ceramic Joining, p. 75. , edited by M. M. Schwartz, ASM International, Ohio; 
Paiva, O.C., Barbosa, M.A., (1997) High Temperature Capillarity, p. 306. , edited by N. Eustathopoulos and N. Sobczak Foundry Research Institute, Cracow, Poland; 
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Yau, T.E.-L., (1992) Werkstoffe und Korrosion, 43, p. 358; 
Sequeira, C.A.C., (1995) British Corrosion Journal, 30, p. 137; 
Uhlig, H.H., (1967) Corrosion and Corrosion Control, , John Wiley \& Sons, New York; 
Paiva, O.C., Barbosa, M.A., (1998) Bioceramics, 11, p. 699. , edited by R. Z. LeGeros and J. LeGeros World Scientific Publishing Co. Pte. Ltd., Singapore
},
	month = {2000///},
	pages = {1165 - 1175},
	publisher = {Kluwer Academic Publishers},
	abstract = {The main aims of the present study are simultaneously to relate tho brazing parameters with the correspondent interfacial microstructure, the resultant mechanical properties and the electrochemical degradation behaviour of commercially pure titanium/alumina brazed joints. A filler metal on the Ag-26.5Cu-3Ti system has been used. Three different brazing temperatures (850, 900 and 950 {\textdegree}C) and three holding times (0.3, 1.2, 2.4 ks) were tested, in order to understand the influence of each combination of brazing temperature holding times, over the final microstructure and properties of the joints. The mechanical properties of the M/C joints were assessed on the basis of bond strength tests carried out using a shear solicitation scheme. The fracture surfaces were studied morphologically and structurally using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction analysis (XRD). The degradation behaviour of the M/C joints was assessed by means of electrochemical techniques. It was found that a brazing temperature of 850 {\textdegree}C and a holding time of 2.4 ks, produces the best results in terms of bond strength, 130{\textpm}16 MPa. The mechanical properties obtained could be explained on the basis of the different compounds identified on the fracture surfaces by XRD. On the other hand, a brazing temperature of 950 {\textdegree}C produces the best results in terms of corrosion rates (lower corrosion current), 3.44{\textpm}0.73 μA cm},
	keywords = {Alumina, Aluminum copper titanide, Brazing filler metals, Ceramic matrix composites, Degradation, Energy dispersive spectroscopy, fracture, Joints (structural components), Scanning electron microscopy, Silver alloys, Titanium, X ray diffraction analysis},
	isbn = {00222461 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033881734\&partnerID=40\&md5=058678288fbb7c85032cae1530352c40},
	author = {Paiva, O.C. and Barbosa, M.A.}
}
@article {2553,
	title = {Concept, design and fabrication of smart orthopedic implants},
	journal = {Medical Engineering and PhysicsMed. Eng. Phys.},
	volume = {22},
	year = {2000},
	note = {Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: MEPHEdoi: 10.1016/S1350-4533(00)00062-XPubMed ID: 11165144Language of Original Document: EnglishCorrespondence Address: Burny, F.; Service d{\textquoteright}Orthopedie-Traumatologie, Cliniques Universitaires Bruxelles, H{\^o}pital Erasme, Brussels, Belgium; email: fburny@ulb.ac.beChemicals/CAS: Biocompatible Materials; Ceramics; Metals; Stainless Steel, 12597-68-1References: Burny, F., Donkerwolcke, M., Moulart, F., Clinical application of the monitoring of orthopedic implants (1993), pp. 138-146. , Burny R, Puers F, editors. Monitoring of orthopedic implants. A biomaterials microelectronics - challenge. Amsterdam: ElsevierBurny, F., Zucman, J., Bourgois, R., Aubriot, J.H., Sedel, J., Utilisation des jauges extensom{\'e}triques pour la mesure de la consolidation des fractures du tibia trait{\'e}es par enclouage centrom{\'e}dullaire. Etude th{\'e}orique et premiers r{\'e}sultats (1971) Acta Orthop Belg, 37, pp. 266-277;Moulart, F., (1975) R{\'e}{\'e}ducation des fractures hautes du f{\'e}mur. Mesure des d{\'e}formations du mat{\'e}riel d{\textquoteright}ost{\'e}osynthese, , M{\'e}moire de Licence en Kin{\'e}sith{\'e}rapie, Universit{\'e} Libre de Bruxelles; 
Burny, F., Moulart, F., Hinsenkamp, M., Traitement des fractures m{\'e}tastatiques de la region sous-trochant{\'e}rienne. Etude biom{\'e}canique; cas cliniques (1976) Acta Orthop Belg, 42, pp. 31-41; 
Burny, F., Donkerwolcke, M., Telemetry in the musculoskeletal system (1991), pp. 391-394. , Payne PA, editor. Concise encyclopedia of biological and biomedical measurement systems. Oxford: Pergamon PressPuers, R., Burny, F., Biotelemetry in orthopaedics (1992), 3, pp. 285-306. , Muster D. et al., editors. European Materials Research Society Monographs. Biomaterials. Hard Tissue Repair and Replacement, Amsterdam: ElsevierKadefors, R., Kaiser, E., Petersen, I., Energizing implantable transmitters by means of coupled inductance coils (1969) IEEE Trans Biomed Eng, 16 BME, pp. 177-183; 
Ko, W.H., Liang, S.P., Fung, C.D.F., Design of radio-frequency powered coils for implant intruments (1977) Med Biol Eng Comput, 15, pp. 634-640; 
Donaldson, N., De, N., Perkins, T.A., Analysis of resonant coupled coils in the design of radio frequency trancutaneous links (1983) Med Biol Eng Comput, 21, pp. 612-627; 
Grossman, D.G., Machining a machinable glass-ceramic (1978) Vacuum, 28, pp. 55-61; 
Van Schuylenbergh, K., Puers, R., The influence of moisture leakage on implant strain measurements with resistive strain gauges (1993), pp. 125-137. , Burny R, Puers F, editors. Monitoring of orthopedic implants. A biomaterials microelectronics - challenge. Amsterdam: ElsevierWrangl{\'e}n, G., (1985) An introduction to corrosion and protection of metals, , London: Chapman \& Hall; 
Barbosa, M.A., Surface layers and the reactivity of metallic implants (1992), pp. 257-284. , Muster D, editor. Biomaterials - hard tissue repair and replacement, E-MRS Monographs. North-Holland: Elsevier ScienceNicholas, M.G., Active metal brazing (1986) British Ceramic Transactions Journal, 85, pp. 144-146; 
Pattee, H.E., Joining ceramics to metals and other materials (1972) Welding Research Council Bulletin, 178, pp. 2435-2442. , New York; 
Nicholas, M.G., Crispin, R.M., Brazing ceramics with alloys containing titanium (1989) Ceramic Engineering and Science Proceedings, 10, pp. 1602-1612; 
Rodes, F., Zardini, C., Devall, Y., Puers, R., Van Schuylenbergh, K., Burny, F., Donkerwolcke, M., Moulart, F., Direct digitization of low level transducer outputs for low power intracorporal telemetry systems (1993), pp. 401-409. , Mancini P, Fioretti S, editors. 12th Int. Symposium on Biotelemetry, Ancona. Pisa, Italy: Universitaria Litografia FeliciFlack, F.C., James, E.D., Schlapp, D.M., Mutual inductance of air-cored coils: Effect on design of radio-frequency coupled implants (1971) Med Biol Eng Comput, 9, pp. 79-85; 
},
	month = {2000///},
	pages = {469 - 479},
	abstract = {Extensive clinical experience has been built up using orthopaedic implants instrumented with strain gauges connected to a Wheatstone bridge by means of percutaneous leads. This research showed the medical relevance of the monitoring of the deformation of implants as a powerful tool to evaluate nursing and rehabilitation exercises, for tracing dangerous overloads and anticipating implant failure and also to observe the healing process. The IMPACT 3500 project focuses on the instrumentation of femoral implants with on board sensors: regular Benoist-Girard implants have been modified, to contain a {\textquoteright}sensing cell{\textquoteright}, and thoroughly tested in vitro and in vivo. The implant deformations are measured with resistive strain gauges, and the signal is transferred to a personal computer for processing and display, via a hard wired connection, or via a telemetry system. Two fully implantable wireless designs, called Linkstrain and Sealstrain, are powered from the outside by magnetic induction. As Sealstrain contains the whole telemetric system in its cavity, the highest miniaturization was required; this seriously deteriorates the efficiency of the inductive power link. {\textcopyright} 2001 IPEM.},
	keywords = {article, atomic absorption spectrometry, Biocompatible Materials, biomechanics, body movement, Bone Plates, ceramics, compression, Corrosion, Failure of implants, Femoral Fractures, femur, Fracture healing, healing, Humans, materials testing, metal, metal implantation, Metals, microcomputer, Microscopy, Electron, Scanning, Monitoring, Physiologic, Orthopedic implants, orthopedic surgery, priority journal, Prostheses and Implants, Prosthesis Design, prosthesis failure, Rehabilitation, Scanning electron microscopy, sensor, Sensors, Smart implants, Spectrum Analysis, Stainless steel, strain gauge transducer, Strain measurements, Stress, Mechanical, Telemetry, Weight-Bearing},
	isbn = {13504533 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-20244372369\&partnerID=40\&md5=456fea60c077d9bd4569169438349a07},
	author = {Burny, F. and Donkerwolcke, M. and Moulart, F. and Bourgois, R. and Puers, R. and Van Schuylenbergh, K. and Barbosa, M. and Paiva, O. and Rodes, F. and B{\'e}gueret, J.B. and Lawes, P.}
}
@article {2564,
	title = {Fetal heart rate baselines in twins: Interobserver agreement in antepartum estimation},
	journal = {Journal of Reproductive Medicine for the Obstetrician and GynecologistJ. Reprod. Med. Obstet. Gynecol.},
	volume = {45},
	year = {2000},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: JRPMAPubMed ID: 10710739Language of Original Document: EnglishCorrespondence Address: Bernardes, J.; Depto. de Ginecologia e Obstetricia, Faculdade de Medicina do Porto, Al. Prof. Hernani Monteiro, 4200 Porto, Portugal; email: Jbernardes@mail.telepac.ptReferences: Sherman, S.J., Kovacs, B.W., Medearis, A.L., Nonstress test assessment of twins (1992) J Reprod Med, 37, pp. 804-808;Grant, J.M., The fetal heart rate is normal, isn{\textquoteright}t it?: Observer agreement of categorical assessments (1991) Lancet, 337, pp. 215-218; 
Cibils, L.A., On intrapartum fetal monitoring (1996) Am J Obstet Gynecol, 174, pp. 1382-1389; 
Bernardes, J., Costa-Pereira, A., Ayres-De-Campos, D., Evaluation of interobserver agreement of cardiotocograms (1997) Int J Gynecol Obstet, 57, pp. 33-37; 
Bernardes, J., Costa-Pereira, A., Van Geijn, H.P., A more objective fetal heart rate baseline estimation (1996) Br J Obstet Gynaecol, 103, pp. 714-715; 
Bernardes, J., Ayres-De-Campos, D., Costa-Pereira, A., Objective computerized fetal heart rate analysis (1998) Int J Gynecol Obstet, 62, pp. 141-147; 
Bland, J.M., Altman, D.G., Statistical methods for assessing two methods of clinical measurement (1986) Lancet, 11, pp. 307-310; 
Armitage, P., Berry, G., (1994) Statistical Methods in Medical Research, pp. 273-276. , Oxford, Blackwell Science Ltd; 
Khan, K.S., Chien, P.F.W., Honest, M.R., Evaluating measurement variability of clinical investigations: The case of ultrasonic estimation of urinary bladder volume (1997) Br J Obstet Gynaecol, 104, pp. 1036-1043; 
Devoe, L.D., Carlton, E., Prescott, P., Neural network prediction of nonstress test results: How often should we perform nonstress tests? (1995) Am J Obstet Gynecol, 173, pp. 1128-1131; 
Bernardes, J., Ayres De Campos, D., Costa-Pereira, A., Baseline estimation in typical fetal heart rate patterns using different computerized methods (1997) Acta Obstet Gynecol Scand (Suppl), 76, p. 56; 
},
	month = {2000///},
	pages = {105 - 108},
	abstract = {OBJECTIVE: To assess interobserver agreement in antepartum estimation of fetal heart rate (FHR) baselines in twins. STUDY DESIGN: Two residents and one specialist in obstetrics and gynecology, all with special interest in FHR monitoring, independently estimated baselines in 162 consecutive antepartum FHR tracings recorded in 24 twins. Tracings were obtained with a dual-channel fetal monitor for the simultaneous recording of both twins{\textquoteright} heart rates. Baselines were estimated, as single values corresponding to the mean of the lowest stable FHR segment, in the absence of fetal movements and uterine contractions, within physiologic limits (110-150 beats per minute [bpm]). If these criteria were not met, the possibility of persistent bradycardia or tachycardia was considered, and if this was confirmed in a tracing with at least 40 minutes, a baseline < 110 or > 150 bpm was chosen. Interobserver agreement was assessed by the proportions of agreement (PA), kappa statistic (K) and intraclass correlation coefficient (ICCC), with 95\% confidence intervals (CIs). RESULTS: Interobserver agreement was excellent, with a PA of 0.90 (95\% CI: 0.89-0.91), K of 0.88 (95\% CI: 0.84-0.92) and ICCC of 0.91 (95\% CI: 0.88-0.94). CONCLUSION: Interobserver agreement in antepartum estimation of fetal heart rate baselines in twins was excellent with the baseline concept used in this study.},
	keywords = {adult, article, clinical article, Electrocardiography, Female, Fetal heart rate, Fetal monitoring, fetus, fetus heart rate, fetus monitoring, Heart rate variability, Heart Rate, Fetal, human, Humans, Internship and Residency, observer variation, Obstetrics, Pregnancy, Prenatal Care, priority journal, Professional Competence, Twins},
	isbn = {00247758 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034002107\&partnerID=40\&md5=82b6075749b0531b00b1195b539b4bb3},
	author = {Bernardes, J. and Costa-Pereira, A. and Calejo, L. and Ayres-de-Campos, D. and Patr{\'\i}cio, B. and Pereira-Leite, L.}
}
@article {2568,
	title = {Histological effects of iron accumulation on mice liver and spleen after administration of a metallic solution},
	journal = {BiomaterialsBiomaterials},
	volume = {20},
	year = {1999},
	note = {Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(99)00124-6PubMed ID: 10555088Language of Original Document: EnglishCorrespondence Address: Pereira, M.C.; Departamento de Engenharia Quimica, Faculdade de Engenharia, Universidade do Porto, Rua dos Bragas, 4050-123 Porto, Portugal; email: mcsp@fe.up.ptChemicals/CAS: Iron, 7439-89-6; SolutionsReferences: Rheinold, J.B., Trace elements - A selective survey (1975) Clin Chem, 21, p. 476;Crichton, R.R., Ward, R.J., Structure and molecular biology of iron binding-proteins and the regulation of free iron pools (1992) Iron and Human Diseases, pp. 23-75. , R.B. Lauffer. Boca Raton: CRC Press Inc; 
Lynch, S.R., Lipschitz, D.A., Bothwell, T.H., Charlton, R.W., Iron and the reticulo-endothelial system (1974) Iron in Biochemistry and Medicine, p. 563. , A. Jacobs, \& M. Worwood. London: Academic Press; 
Black, J., Mineral metabolism (1981) Biological Performance of Materials: Fundamentals of Biocompatibility. Biomedical Engineering and Instrumentation, 8, pp. 158-168. , R. Plonsey, Katz J.L. New York: Marcel Dekker; 
Sutow, E.J., Pollack, S.R., The biocompatibility of certain stainless steels (1981) Biocompatibility of Clinical Implant Materials, pp. 77-98. , D.F. Williams. Boca Raton, FL: CRC Press; 
Winter, G.D., Tissue reactions to metallic wear and corrosion products in human patients (1974) J Biomed Mater Res Symp, 5, pp. 11-26; 
Winter, G.D., Wear and corrosion products in tissues and the reactions they provoke (1976) Biocompatibility of Implant Materials, pp. 28-54. , In: Williams, DF, editor London; 
Ferguson A.B., Jr., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J Bone Jt Surg a, 42, pp. 77-90; 
Pohler, O.E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, pp. 196-197. , In: Rubin LR, editor London: The C.V. Mosby Company; 
Lux, F., Zeisler, R., Investigations of the corrosive deposition of components of metal implants and the behaviour of biological trace elements in metallosis tissue by means of instrumental multi-element activation analysis (1974) J Radioanal Chem, 19, pp. 289-297; 
Traisnel, M., Le Maguer, D., Hildebrand, H.F., Iost, A., Corrosion of surgical implants (1990) Clin Mater, 5, pp. 309-318; 
Gosztonyi, E., (1983) Iron Metabolism, p. 339. , New York: Plenum Press; 
Weinberg, E.D., Cellular acquisition of iron and the iron-withholding defense against microbial and neoplastic invasion (1992) Iron and Human Diseases, pp. 179-207. , R.B. Lauffer. Boca Raton, FL: CRC Press Inc; 
Pereira, M.L., Pereira, M.C., Sousa, J.P., Evaluation of nickel effects on mouse liver: Tissue response and metal ion accumulation (1995) Biomed Lett, 52, pp. 235-244; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Evaluation of nickel toxicity on liver, spleen and kidney of mice after administration of high-dose metal ion (1998) J Biomed Mater Res, 40, pp. 40-47; 
Pereira, M.C., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using microelectrodes (1997) Electroanalysis, 9, pp. 150-154; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping measurements of iron accumulation in mice kidney using microelectrodes and histological features (1998) J Trace Elements Med Biol, 12, pp. 50-55; 
Van Den Berg, C.M.G., Huang, Z.Q., Determination of iron in seawater using cathodic stripping voltammetry proceeded by adsorptive collection with the hanging mercury drop electrode (1984) J Electroanal Chem, 177, pp. 269-280; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 223-247. , A.C. Fraker, \& C.D. Griffin. Philadelphia: American Society for Testing and Materials; 
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Britton, S.R., Ramm, G.A., Olynyk, J., Singh, R., O{\textquoteright}Neill, R., Bacon, B.R., Pathophysiology of iron toxicity (1994) Progress in Iron Research, pp. 239-253. , C. et al. Hershko. New York: Plenum Press
},
	month = {1999///},
	pages = {2193 - 2198},
	publisher = {Elsevier Science Ltd},
	abstract = {Special attention has been focused on the toxicity of some metallic species released from implanted materials, which accumulate in vital organs over long periods of time. A set of experiments with mice was designed to investigate the individual effects caused by iron in the liver and spleen. Histological features of these organs were evaluated and slight morphological changes were observed during the treatment time suggesting a negative correlation with the duration of the iron treatment. In addition, to associate the histological changes in the organs with iron accumulation an electrochemical method, adsorptive stripping voltammetry, was chosen to quantify the iron levels in these mentioned organs. The accuracy of the proposed method was checked by atomic absorption spectrometry. Both organs showed elevated concentrations of iron, nearly twofold, 7 days after iron administration compared to control organs.Special attention has been focused on the toxicity of some metallic species released from implanted materials, which accumulate in vital organs over long periods of time. A set of experiments with mice was designed to investigate the individual effects caused by iron in the liver and spleen. Histological features of these organs were evaluated and slight morphological changes were observed during the treatment time suggesting a negative correlation with the duration of the iron treatment. In addition, to associate the histological changes in the organs with iron accumulation an electrochemical method, adsorptive stripping voltammetry, was chosen to quantify the iron levels in these mentioned organs. The accuracy of the proposed method was checked by atomic absorption spectrometry. Both organs showed elevated concentrations of iron, nearly twofold, 7 days after iron administration compared to control organs.
},
	keywords = {Absorption spectroscopy, accuracy, Adsorption, Adsorptive stripping voltametry, Adsorptive stripping voltammetry, animal experiment, animal model, animal tissue, Animalia, Animals, article, atomic absorption spectrometry, Atomic spectroscopy, bioaccumulation, biomaterial, controlled study, Corrosion, electrochemistry, histology, histopathology, Implants (surgical), Injections, Subcutaneous, Iron, iron metabolism, Kinetics, Liver, liver toxicity, male, metal implantation, Mice, morphology, mouse, nonhuman, Organ Size, potentiometry, priority journal, quantitative assay, Solutions, Spleen, Stainless steel, tissue distribution, toxicity},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032855084\&partnerID=40\&md5=2e7274d8d0372ff55881c2d9de94c54b},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2570,
	title = {Individual study of chromium in the stainless steel implants degradation: An experimental study in mice},
	journal = {BioMetalsBioMetals},
	volume = {12},
	year = {1999},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: BOMEEPubMed ID: 10581692Language of Original Document: EnglishCorrespondence Address: Do Carmo Pereira, M.; Departamento de Engenharia Quimica, FEUP, Rua dos Bragas, 4050-123 Porto, Portugal; email: mcsp@fe.up.ptChemicals/CAS: Biocompatible Materials; Chromium, 7440-47-3; Stainless Steel, 12597-68-1References: Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8;Black, J., Maitin, E.C., Gelman, H., Morris, D.M., Serum concentrations of chromium, cobalt and nickel after total hip replacement: A six month study (1983) Biomaterials, 4, pp. 160-164; 
Brien, W.W., Salvati, E.A., Healey, J.H., Bansal, M., Ghelman, B., Betts, F., Osteogenic sarcoma arising in the area of a total hip replacement (1990) J Bone Joint Surg, 72 A, pp. 1097-1099; 
Brown, S.A., Farnsworth, U., Merritt, K., Crowe, T.D., In vitro and in vivo metal ion release (1988) J Biomed Mater Res, 22, pp. 321-338; 
Brown, S.A., Zhang, K., Merritt, K., Payer, J.H., In vivo transport and excretion of corrosion products from accelerated anodic corrosion of porous coaled 1-75 alloy (1993) J Biomed Mater Res, 27, pp. 1007-1017; 
Burrows, D., (1983) Chromium: Metabolism and Toxicity, , CRC Press, Boca Raton, Florida; 
Dobbs, H.S., Minski, M.J., Metal ion release after total hip replacement (1980) Biomaterials, 1, pp. 193-198; 
Kargacin, B., Squibb, K.S., Cosentino, S., Zhitkovich, A., Costa, M., Comparison of the uptake and distribution of chromate in rats and mice (1993) Biol Trace Element Res, 36, pp. 307-318; 
Katz, S.A., Salem, H., The toxicology of chromium with respect to its chemical speciation: A review (1993) J Appl Toxicol, 13, pp. 217-224; 
Katz, S.A., Salem, H., (1994) The Biological and Environmental Chemistry of Chromium, , VCH Publishers; 
Langard, S., Hensten-Pettersen, A., Chromium toxicology (1981) Systemic Aspects of Biocompatibility, p. 143. , Williams DF. eds. Boca Raton, Florida, CRC Press; 
Martin, A., Bauer, T.W., Manley, M.T., Marks, K.E., Osteosarcoma at the site of total hip replacement (1988) J Bone Joint Surg, 70 A, pp. 1561-1566; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , Fraker AC. Griffin CD. eds. Philadelphia, American Society for Testing and Materials; 
Merritt, K., Brown, S.A., Release of hexavalent chromium from corrosion of stainless steel and cobalt-chromium alloys (1995) J Biomed Mater Res, 29, pp. 627-633; 
Merritt, K., Brown, S.A., Sharkey, N.A., Blood distribution of nickel, cobalt, and chromium following intramuscular injection into hamsters (1984) J Biomed Mater Res, 18, pp. 991-1004; 
Merritt, K., Brown, S.A., Sharkey, N.A., The binding of metal salts and corrosion products to cells and proteins in vitro (1984) J Biomed Mater Res, 18, pp. 1005-1015; 
Merritt, K., Crowe, T.D., Brown, S.A., Elimination of nickel, cobalt, and chromium following repeated injections of high dose metal salts (1989) J Biomed Mater Res, 23, pp. 845-862; 
Merritt, K., Fedele, C.D., Brown, S.A., Chromium 6+ or 3+ release during corrosion; And in vivo distribution (1992) Biomaterial- Tissue Interfaces, Advances in Biomaterials, 10, pp. 49-53. , Doherty PJ. et al., eds; 
Pereira, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using microelectrodes (1997) Electroanalysis, 9, pp. 150-154; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping voltammetric measurements of chromium accumulation in mice organs using mercury film microelectrodes (1997) Electroanalysis, 9, pp. 941-944; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Evaluation of nickel toxicity on liver, spleen and kidney of mice after administration of high-dose metal ion (1998) J Biomed Mater Res, 40, pp. 40-47; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping measurements of iron accumulation in mice kidney using microlectrodes and histological features (1998) J Trace Elements Med Biol, 12, pp. 50-55; 
Pohler, O.E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, pp. 158-228. , Rubin LR, ed. The C. V. Mosby Company; 
Ross, M.H., Rowrell, L.J., (1985) Histology a Text and Atlas, , Williams \& Wilkins; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials; Second Symposium, pp. 223-247. , Fraker AC, Griffin CD, eds. American Philadelphia, Society for Testing and Materials; 
Sunderman F.W., Jr., Hopfer, S.M., Swift, T., Cohalt, chromium, and nickel concentrations in body fluids of patients with porous-coated knee or hip prostheses (1989) J Orthop Res, 7, pp. 307-315; 
Yang, J., Black, J., Competitive binding of chromium, cobalt and nickel to serum proteins (1994) Biomaterials, 15, pp. 262-268; 
Young, E., Houwing, R.H., Patch test results with standard allergens over a decade (1987) Contact Dermatitis, 17, pp. 104-107
},
	month = {1999///},
	pages = {275 - 280},
	abstract = {To study the accumulation and the histological effects in mice organs caused by hexavalent chromium, one of the corrosion products released from AISI 316L stainless steel implants, mice groups were subcutaneously injected with a metallic solution of chromium during a certain period of time. Similar injections were made with HBSS (Hank{\textquoteright}s Balanced Salt Solution) in other groups to be used as controls. The levels of chromium found in the liver, kidney and spleen of the control and the treated animals were obtained by atomic absorption spectrometry (AAS) and were compared to those obtained by AdSV (adsorptive stripping voltammetry) to test the accuracy of the results. During the experimental period, the liver and spleen showed a progressive and significant accumulation of chromium whereas in the kidney the significant accumulation found after the first week practically remained unchanged during the four weeks. Apparently, the histological analysis of these tissues did not evidence any relevant morphological alteration induced by the chromium accumulations during the four weeks of treatment.},
	keywords = {animal experiment, animal tissue, Animalia, Animals, article, atomic absorption spectrometry, Biocompatible Materials, biomaterial, body weight, Chromium, controlled study, Corrosion, histology, histopathology, implant, Injections, Subcutaneous, Kidney, Liver, liver level, male, Mice, Mice organs, Mice, Inbred Strains, mouse, nonhuman, potentiometry, Prostheses and Implants, Spleen, Stainless steel, tissue distribution},
	isbn = {09660844 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032840626\&partnerID=40\&md5=e81da6070ec455e49604d3773033d58d},
	author = {Pereira, M.D.C. and Pereira, M.D.L. and Sousa, J.P.}
}
@article {2588,
	title = {Adsorptive stripping measurements of iron accumulation in mice kidney using microelectrodes and histological features},
	journal = {Journal of Trace Elements in Medicine and BiologyJ. Trace Elem. Med. Biol.},
	volume = {12},
	year = {1998},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JTEBFPubMed ID: 9638613Language of Original Document: EnglishCorrespondence Address: Sousa, J.P.; INEB-Inst. de Engenharia Biomedica, Departamento de Engenharia Quimica, Faculdade Engenharia Univ. do Porto, Praca do Coronel Pacheco, 1, 4050 Porto, PortugalChemicals/CAS: Iron, 7439-89-6; Mercury, 7439-97-6References: Traisnel, M., Le Maguer, D., Hildebrand, H.F., Iost, A., Corrosion of surgical implants (1990) Clin. Mater., 5, pp. 309-318;Sutow, E.J., Pollack, S.R., The biocompatibility of certain stainless steels (1981) Biocompatibility of Clinical Implant Materials, 1, pp. 77-98. , Williams, D.F. eds CRC Press, Boca Raton, Florida; 
Ferguson Jr., A.B., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J. Bone Joint Surg., 42 A, pp. 77-90; 
Winter, G.D., Wear and corrosion products in tissues and the reactions they provoke (1976) Biocompatibility of Implant Materials, pp. 28-54. , Williams, D.F. eds London; 
Lux, F., Zeisler, R., Investigations of the corrosive deposition of components of metal implants and the behaviour of biological trace elements in metallosis tissue by means of instrumental multi-element activation analysis (1974) J. Radioanal. Chem., 19, pp. 289-297; 
Merritt, K., Brown, S.A., Sharkey, N.A., Blood distribution of nickel, cobalt, and chromium following intramuscular injection into hamsters (1984) J. Biomed. Mater. Res., 18, pp. 991-1004; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , Fraker, A.C., Griffin, C.D. eds American Society for Testing and Materials, Philadelphia; 
K. Merritt, K.A., Brown, S.A., Metal sensitivity reactions to bone screws in rabbits (1982) Clinical Applications of Biomaterials, pp. 85-93. , John Wiley \& Sons Ltd; 
Woodman, J.L., Black, J., Jimenez, S., Organometallic corrosion products of 316L SS HS-21 in serum: In vitro and in vivo comparison in an acute rat model (1982) Biomaterials 1980, pp. 245-250. , Winter, G.D., Gibbons, D.F., Plenk, Jr., H. John Wiley \& Sons Ltd; 
Merritt, K., Crowe, T.D., Brown, S.A., Elimination of nickel, cobalt, and chromium following repeated injections of high dose metal salts (1989) J. Biomed. Mater. Res., 23, pp. 845-862; 
Pereira, M.L., Pereira, M.C., Sousa, J.P., Evaluation of nickel effects on mouse liver: Tissue response and metal ion accumulation (1995) Biomedical Letters, 52, pp. 235-244; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Evaluation of nickel toxicity on liver, spleen and kidney of mice after administration of high-dose metal ion (1997) J. Biomed. Mater Res., pp. XXXXXX. , in press; 
Schafer, B.A.I., Cheron, R.G., Dluhy, R., Cooper, B., Gleason, R.E., Soeldner, J.S., Bunn, H.F., Clinical consequences of acquired transfusional iron overload in adults (1981) N Engl. J. Med., 304, p. 319; 
Black, J., Mineral metabolism (1981) Biological Performance of Materials: Fundamentals of Biocompatibility. Biomedical Engineerins and Instrumentation Series, pp. 158-168. , Plonsey R., Katz, J.L. Marcel Dekker; 
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Perejra, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using mercury microelectrodes (1996) Electroanalysis, 9, pp. 150-154; 
Van Den Berg, C.M.G., Huang, Z.Q., Determination of iron in seawater using cathodic stripping voltammetry preceded by adsorptive collection with the hanging mercury drop electrode (1984) J. Electroanal. Chem., 177, pp. 269-280; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell cultures medium by adsorptive cathodic stripping voltammetry with mercury microelectrodes (1997) Electroanalysis, 9; 
Wang, J., Mannino, S., Application of adsorptive stripping voltammetry to the speciation and determination of iron (III) and total iron in wines (1989) Analyst, 114, pp. 643-645; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 223-247. , Fraker, A.C. and Griffin. Eds. American Society for Testing Materials; 
Pereira, M.C., Sousa, J.P., Determination of total iron in biological sample solutions with mercury microelectrodes (1996) Portugaliae Electrochimica Acta, 14, pp. 273-278; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping voltammetric measurements of chromium accumulation in mice organs using mercury microelectrodes (1997) Electroanalysis, 9, pp. 941-944
},
	month = {1998///},
	pages = {50 - 55},
	abstract = {The toxic effects caused by iron in kidney was performed on experimental studies with mice following administration of a metallic solution of this specie to simulate the iron corrosion products of a metallic implant. To quantify the total levels of iron present in this organ, an electrochemical method was chosen based on the application of square wave voltammetry using adsorptive collection of iron-catechol on a mercury film microelectrode (MFM). The optimal working conditions to produce a very stable and reproducible iron peak in the digested kidney samples were found to be pH=7.2 provided by 8.0 mmol/L PIPES buffer, catechol concentration of 3.0x10},
	keywords = {, biomaterials, Adsorptive stripping, animal experiment, animal model, animal tissue, Animals, article, atomic absorption spectrometry, biomaterial, controlled study, electrochemical analysis, electrochemistry, histology, Iron, iron absorption, Kidney, male, Mercury, metal implantation, Mice, microelectrode, Microelectrodes, mouse, nonhuman, potentiometry, priority journal, Toxicology},
	isbn = {0946672X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031746863\&partnerID=40\&md5=f85b47bb916eab32df5be2219498793f},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2587,
	title = {Evaluation of nickel toxicity on liver, spleen, and kidney of mice after administration of high-dose metal ion},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {40},
	year = {1998},
	note = {Cited By (since 1996): 27Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(199804)40:1<40::AID-JBM5>3.0.CO;2-RPubMed ID: 9511097Language of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Instituto de Engenharia Biomedica, Faculdade de Engenharia, Universidade do Porto, Praca Coronel Pacheco, 1, 4050 Porto, PortugalChemicals/CAS: Nickel, 7440-02-0References: Cigada, A., Rondelli, G., Vicentini, B., Brunella, F., Dallaspezia, G., Corrosion behavior of high nitrogen stainless steels for biomedical applications (1994) Compatibility of Biomedical Implants, pp. 185-195. , P. Kovacs and N. S. Istephanous (eds.), The Electrochemical Society, Inc., Pennington, New Jersey;Bumgardner, J.D., Lucas, L.C., An evaluation of the corrosion properties and cellular compatibility of nickel-based dental casting alloys (1994) Compatibility of Biomedical Implants, pp. 241-252. , P. Kovacs and N. S. Istephanous (eds.), The Electrochemical Society, Inc., Pennington, New Jersey; 
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Tracana, R.B., Sousa, J.P., Carvalho, G.S., Mouse inflammatory response to stainless steel corrosion products (1994) J. Mater. Sci. Mater. Med., 5, pp. 596-600; 
Jacks, J.J., Skiper, A.K., Black, J., Urban, R.M., Galante, J.O., Release and excretion of metal in patients who have a total hip replacement component made of titanium base alloy (1991) J. Bone Joint Surg., 73 A, pp. 1475-1486; 
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Panigutti, M.A., Merrit, K., Bruner, R.J., Kraay, M.J., Brown, S.A., Correlation of allergy, metal levels, implant alloy, and implant damage in patients undergoing revision joint arthroplastics (1992) Trans. Soc. Biomater., 15, p. 7; 
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(1986) Health Assessment Document for Nickel and Nickel Compounds, , EPA/600/8-83/012FF, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina; 
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Nieboer, E., Rossetto, F.E., Menon, C.R., Toxicology of nickel compounds (1980) Nickel Toxicology, pp. 359-401. , S. S. Brown and F. W. Sunderman, Jr. (eds.), Academic Press, Inc., New York; 
Pereira, M.L., Pereira, M.C., Sousa, J.P., Evaluation of nickel effects on mouse liver: Tissue response and metal ion accumulation (1995) Biomed. Lett., 52, pp. 235-244; 
Pereira, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive stripping voltammetry using mercury microelectrodes (1996) Electroanalysis, 9, pp. 1-5; 
Black, J., Systemic distribution and excretion (1981) Biomedical Engineering and Instrumentation, p. 180. , R. Plonsey and J. L. Katz (eds.), Marcel Dekker, Inc., New York; 
Pohler, O.E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, p. 197. , L. R. Rugin (ed.), The C. V. Mosby Company, St. Louis; 
Ross, M.H., Romrell, L.J., (1989) Histology - A Text and Atlas, , Williams and Wilkins, New York
},
	month = {1998///},
	pages = {40 - 47},
	publisher = {John Wiley \& Sons Inc},
	abstract = {The toxic effects caused by nickel (Ni) per si were explored by performing in vivo studies on mice following subcutaneous administration of a metallic solution of nickel at 1, 2, 3, and 4 weeks to evaluate the side effects of this metal ion when released from stainless steel implants. Other groups were similarly injected with HBSS and used as controls. Accumulation of Ni ions on liver, spleen, and kidney was assessed by an electrochemical method, adsorptive stripping voltammetry (AdSV) using microelectrodes, and atomic absorption spectrometry (AAS). Alterations of those organs induced by Ni ions were studied, showing that several histological changes had been induced. Chemical analysis and histological features indicate that Ni is partially accumulated in the study organs.The toxic effects caused by nickel (Ni) per si were explored by performing in vivo studies on mice following subcutaneous administration of a metallic solution of nickel at 1, 2, 3, and 4 weeks to evaluate the side effects of this metal ion when released from stainless steel implants. Other groups were similarly injected with HBSS and used as controls. Accumulation of Ni ions on liver, spleen, and kidney was assessed by an electrochemical method, adsorptive stripping voltammetry (AdSV) using microelectrodes, and atomic absorption spectrometry (AAS). Alterations of those organs induced by Ni ions were studied, showing that several histological changes had been induced. Chemical analysis and histological features indicate that Ni is partially accumulated in the study organs.
},
	keywords = {Absorption spectroscopy, Adsorptive stripping voltammetry, animal experiment, animal tissue, Animals, article, atomic absorption spectrometry, biomaterial, body weight, controlled study, Dose-Response Relationship, Drug, electrochemistry, Evaluation Studies, histology, Implants (surgical), Kidney, Liver, liver toxicity, Living systems studies, Mice, Microelectrodes, mouse, nephrotoxicity, Nickel, nonhuman, potentiometry, Spectrophotometry, Atomic, Spleen, spleen injury, Stainless steel, toxicity},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032053959\&partnerID=40\&md5=b1b91f83e841302830f88702c7146ae0},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2591,
	title = {In situ characterization of the p-Si/NH                    4F interface during dissolution in the current oscillations regime
},
	journal = {Journal of the Electrochemical SocietyJ Electrochem Soc},
	volume = {145},
	year = {1998},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: JESOALanguage of Original Document: EnglishCorrespondence Address: Cattarin, S.; Ist. Polarografia ed E., C. N. R., 35100 Padova, ItalyReferences: Zhang, X.G., Collins, S.D., Smith, R.L., (1989) This Journal, 136, p. 1561;Smith, R.L., Collins, S.D., (1992) J. Appl. Phys., 71, pp. R1; 
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},
	month = {1998///},
	pages = {498 - 502},
	abstract = {Several physicochemical properties of the p-Si/NH                        4F interface have been monitored by in situ techniques in the regime of current oscillations. Comparison of evolution of infrared absorption, microwave reflectivity, electrode admittance, hydrogen evolution, and electron injection rate shows interesting correlations. An integrated description of the processes involved is attempted on the basis of the current models for the Si/acidic fluoride interface.
},
	keywords = {Ammonium compounds, Ammonium fluoride, Dissolution, Electric currents, Electric Impedance, Electrochemical electrodes, Electron injection rate, Hydrogen, Interfaces (materials), Microwave reflectivity, Porous silicon},
	isbn = {00134651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031996606\&partnerID=40\&md5=9b455077d4ee25d640363197ec7367d4},
	author = {Cattarin, S. and Chazalviel, J.-N. and Da Fonseca, C. and Ozanam, F. and Peter, L.M. and Schlichth{\"o}rl, G. and Stumper, J.}
}
@article {2578,
	title = {New starch-based thermoplastic hydrogels for use as bone cements or drug-delivery carriers},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {9},
	year = {1998},
	note = {Cited By (since 1996): 67Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1008944127971Language of Original Document: EnglishCorrespondence Address: Reis, R.L.; INEB-Inst for Biomedical Engineering, Biomaterials Laboratory, Rua do Campo Alegre 823, 4150 Porto, Portugal; email: rgreis@fe.up.ptChemicals/CAS: acrylic acid, 10344-93-1, 79-10-7; alcohol, 64-17-5; benzoyl peroxide, 94-36-0; cellulose acetate, 9004-35-7; poly(methyl methacrylate), 39320-98-4, 9008-29-1; starch, 9005-25-8, 9005-84-9Tradenames: AA, Merck, Germany; BPO, Merck, Germany; Master-Bi Y101U, novamont, Italy; MMA, Merck, GermanyManufacturers: Merck, Germany; novamont, ItalyReferences: Peppas, N.A., Mikos, A.G., (1988) Hydrogels in Medicine and Pharmacy-Fundamentals, 1, p. 1. , edited by N. A. Peppas CRC Press, Boca Raton, FL;Netti, P.A., Shelton, J.C., Revell, P.A., Pirie, C., Smith, S., Ambrosio, L., Nicolais, L., Bonfield, W., (1993) Biomaterials, 14, p. 1098; 
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},
	month = {1998///},
	pages = {825 - 833},
	abstract = {The development of new biodegradable hydrogels, based on corn starch/cellulose acetate blends, produced by free-radical polymerization with methyl methacrylate monomer (MMA) and/or an acrylic acid monomer (AA), is reported. The polymerization was initiated by a redox system consisting of a benzoyl peroxide and 4-dimethlyaminobenzyl alcohol at low temperature. These hydrogels may constitute an alternative to the materials currently used as bone cements or drug-delivery carriers. Swelling studies were carried out, as a function of pH and temperature, in buffered solutions. The xerogels were further characterized by Fourier transform-infrared spectroscopy. Tensile and compression tests, and dynamic mechanical thermal analysis were used to assess the mechanical performance of the developed materials. The fracture surfaces were observed by scanning electron microscopy. The developed materials are sensitive to the pH, showing a clear reversible transition in a relatively narrow interval of pH, which is just in the range of physiological conditions. These properties make the materials developed in this study very promising for biomedical applications. Fickian-type diffusion is the mechanism predominant in these systems, except for the composition with a higher concentration of AA, that corresponds to the most desirable kinetical behavior for controlled release (case II-transport mechanism). Furthermore, the results obtained in the mechanical tests are in the range of those reported for typical PMMA bone cements, showing that it is possible to develop partially degradable cements with an adequate mechanical behavior.},
	keywords = {acrylic acid, alcohol, article, benzoyl peroxide, Biodegradation, bone cement, buffer, cellulose acetate, compression, Diffusion, drug delivery system, dynamics, fracture, free radical, hydrogel, infrared spectroscopy, Kinetics, low temperature, mechanical stress, oxidation reduction reaction, pH, poly(methyl methacrylate), Polymerization, priority journal, Scanning electron microscopy, Starch, tensile strength, Thermal analysis},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032424837\&partnerID=40\&md5=b06120f87bacd9ba817763d9e06da3ca},
	author = {Pereira, C.S. and Cunha, A.M. and Reis, R.L. and V{\'a}zquez, B. and San Rom{\'a}n, J.}
}
@article {2583,
	title = {Objective computerized fetal heart rate analysis},
	journal = {International Journal of Gynecology and ObstetricsInt. J. Gynecol. Obstet.},
	volume = {62},
	year = {1998},
	note = {Cited By (since 1996): 23Export Date: 21 November 2012Source: ScopusCODEN: IJGOAdoi: 10.1016/S0020-7292(98)00079-4PubMed ID: 9749885Language of Original Document: EnglishCorrespondence Address: Bernardes, J.; Departmento Ginecologia Obstetricia, Faculdade Medicina-Hospital S. Joao, Porto, Portugal; email: sisporto@mail.telepac.ptReferences: Bernardes, J., Costa-Pereira, A., Ayres-De-Campos, D., Van Geijn, H.P., Pereira-Leite, L., Evaluation of interobserver agreement of cardiotocograms (1997) Int J Gynecol Obstet, 57 (1), pp. 33-37;Bernardes, J., Costa-Pereira, A., Van Geijn, H.P., Pereira-Leite, L., A more objective fetal heart rate baseline estimation (1996) Br J Obstet Gynaecol, 103, pp. 714-715; 
Grant, A., Principles for clinical evaluation of methods of perinatal monitoring (1984) J Perinat Med, 12, pp. 227-231; 
Bernardes, J., Moura, C., Marques-De-S{\'a}, J.P., Pereira-Leite, L., Van Geijn, H.P., The Porto System (1994) A Critical Appraisal of Fetal Surveillance, pp. 315-324. , In: van Geijn HP, Copray FJA, editors. Amsterdam: Elsevier Science; 
Rooth, G., Huch, A., Huch, R., Guidelines for the use of fetal monitoring. FIGO news (1987) Int J Gynaecol Obstet, 25, pp. 159-167; 
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Stigsby, B., Nielson, P.V., Nickelsen, C., Nim, J., Computer assessment of the intrapartum cardiotocogram. I. Methods of data reduction and diagnostic procedure (1988) Acta Obstet Gynecol Scand, 67, pp. 455-460
},
	month = {1998///},
	pages = {141 - 147},
	abstract = {Objective: To assess the validity of a computerized methodology for cardiotocogram analysis based on a recently described reproducible visual estimation of the baseline. Methods: Forty-two antepartum and 43 intrapartum cardiotocograms (CTGs) acquired by a personal computer were selected. Antepartum tracings were performed in the 48 h that preceded an elective cesarean section, and intrapartum tracings were performed until delivery. FHR baselines were estimated by an expert, according to an objective and reproducible methodology. Using these baselines, automated detection of accelerations and decelerations and estimation of variability was performed by the personal computer. A quantitative adaptation of the FIGO guidelines for fetal monitoring was used to classify tracings. Perinatal outcome was classified according to the Apgar score and umbilical arterial pH. Validity was then assessed by the proportions of agreement (PA), kappa statistic (κ), sensitivity and specificity, with 95\% confidence intervals (95\% CI). Cases showing a disagreement between CTG and perinatal classification were reviewed and an adjustment in baseline definition was tested. Results: The initial overall PA and κ between CTG and perinatal classification were, respectively, 0.79 (95\% CI: 0.69-0.87) and 0.62 (95\% CI: 0.41-0.83). The overall PA and κ, after baseline adjustment were, respectively, 0.89 (95\% CI: 0.81-0.95) and 0.78 (95\% CI: 0.58-0.98). Sensitivities and specificities ranged between 79\% (95\% CI: 60-92\%) and 100\% (95\% CI: 95-100\%). Conclusions: Good clinical prediction may be possible with an objective methodology for cardiotocogram analysis based on a recently described reproducible baseline estimation.},
	keywords = {Apgar score, arterial pH, article, Cardiotocography, cesarean section, computer analysis, Computerized analysis, Female, Fetal heart rate, fetus, fetus heart rate, fetus monitoring, Heart Rate, Fetal, human, Humans, Image Processing, Computer-Assisted, practice guideline, Predictive Value of Tests, Pregnancy, priority journal, umbilical artery, validity},
	isbn = {00207292 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031873609\&partnerID=40\&md5=2b885fbc95cba06c5f31f417216ebba2},
	author = {Bernardes, J. and Ayres-de-Campos, D. and Costa-Pereira, A. and Pereira-Leite, L. and Garrido, A.}
}
@article {2602,
	title = {Adsorptive Stripping Voltammetric Measurements of Chromium Accumulation in Mice Organs Using Mercury Film Microelectrodes},
	journal = {ElectroanalysisElectroanalysis},
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	year = {1997},
	note = {Cited By (since 1996): 19Export Date: 21 November 2012Source: ScopusCODEN: ELANELanguage of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Inst. de Engenharia Biom{\'e}dica, Depto. de Engenharia Qu{\'\i}mica, Universidade do Porto, Pra{\c c}a do Coronel Pacheco, 1, P-4050 Porto, PortugalReferences: Katz, S.A., Salem, H., (1994) The Biological and Environmental Chemistry of Chromium, , VCH, Weinheim ch. 5;Black, J., (1988) J. Bone Joint Surg., 70 B, p. 517; 
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},
	month = {1997///},
	pages = {941 - 944},
	abstract = {Chromium is the metallic species of most concern with the use of stainless steel implants. To study the distribution of chromium in mice organs with time, several animals were subcutaneously injected with 2.70 {\texttimes} 10},
	keywords = {Adsorptive stripping voltammetry, Biomaterials, Chromium, Microelectrodes},
	isbn = {10400397 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0000379196\&partnerID=40\&md5=9521717895db74058cd9402ada6394b3},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2612,
	title = {Investigation of the dissolution of the bioceramic hydroxyapatite in the presence of titanium ions using ToF-SIMS and XPS},
	journal = {BiomaterialsBIOMATERIALS},
	volume = {18},
	year = {1997},
	note = {Cited By (since 1996): 28Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(96)00134-2PubMed ID: 9068892Language of Original Document: EnglishCorrespondence Address: Davies, M.C.; Lab. Biophysics and Surface Analysis, Department Pharmaceutical Sciences, University of Nottingham, Nottingham NG7 2RD, United KingdomChemicals/CAS: Biocompatible Materials; Cations; Ceramics; Durapatite, 1306-06-5; Titanium, 7440-32-6References: Hench, L.L., Wilson, J., Bioceramics (1991) MRS Bull, p. 62. , September;Katz, J.L., Harper, R.A., (1990) Concise Encyclopedia of Medical and Dental Materials, p. 87. , ed. D. F. Williams, Pergamon Press, Oxford; 
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Briggs, D., Analysis of polymer surfaces by SIMS. VII. Homopolymer and copolymer nylons (1987) Org Mass Spectrom, 22, p. 91
},
	month = {1997///},
	pages = {311 - 316},
	publisher = {Elsevier Science Ltd},
	abstract = {Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) analyses have been used to investigate the dissolution of hydroxyapatite in the presence of titanium chloride suggesting the substitution of titanium ions for calcium in the hydroxyapatite structure. The surface analytical data suggest that titanium was incorporated within the solid phase as titanium phosphate. Comparison of relative ion intensities in the ToF-SIMS spectra reveals changes in the composition of the surface chemistry of hydroxyapatite. These relative ion intensities show that the maximum surface uptake of titanium occurred at a solution concentration of 500 ppm titanium chloride for a time of incubation of 30 min. These data correlate with that obtained using XPS highlighting the semi-quantitative information which ToF-SIMS can provide.Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) analyses have been used to investigate the dissolution of hydroxyapatite in the presence of titanium chloride suggesting the substitution of titanium ions for calcium in the hydroxyapatite structure. The surface analytical data suggest that titanium was incorporated within the solid phase as titanium phosphate. Comparison of relative ion intensities in the ToF-SIMS spectra reveals changes in the composition of the surface chemistry of hydroxyapatite. These relative ion intensities show that the maximum surface uptake of titanium occurred at a solution concentration of 500 ppm titanium chloride for a time of incubation of 30 min. These data correlate with that obtained using XPS highlighting the semi-quantitative information which ToF-SIMS can provide.
},
	keywords = {article, Bioceramic hydroxyapatite, Biocompatible Materials, biomaterial, Biomaterials, Cations, ceramic prosthesis, ceramics, Composition, Dissolution, Durapatite, hydroxyapatite, information processing, mass spectrometry, Phosphates, priority journal, Radiography, Secondary ion mass spectrometry, Spectrometry, Mass, Secondary Ion, Spectrometry, X-Ray Emission, Spectrophotometry, Substitution reactions, Surface chemistry, surface property, Surface structure, Time of flight secondary ion mass spectrometry, Time-of-flight secondary ion mass spectrometry, Titanium, Titanium chloride, X ray photoelectron spectroscopy, X-ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031080821\&partnerID=40\&md5=9581c063071547190645f666a211bddb},
	author = {Leadley, S.R. and Davies, M.C. and Ribeiro, C.C. and Barbosa, M.A. and Paul, A.J. and Watts, J.F.}
}
@article {2613,
	title = {Nickel Quantification in Mice Organs by Adsorptive Cathodic Stripping Voltammetry Using Mercury Microelectrodes},
	journal = {ElectroanalysisElectroanalysis},
	volume = {9},
	year = {1997},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: ELANELanguage of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Inst. de Engenharia Biomedica, Depto. de Engenharia Qu{\'\i}mica, Faculdade Engenharia da Univ. do P., Pra{\c c}a do Coronel Pacheco, 1, P-4050 Porto, PortugalReferences: Mark H.B., Jr., (1964) J. Electroanal. Chem., 7, p. 276;Crow, D.R., Rose, M.E., (1979) Electrochim. Acta, 24, p. 41; 
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Wang, J., (1985) Stripping Analysis, , VCH, Deerfield Beach, FL; 
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Montenegro, M.I., Queir{\'o}s, M.A., Dashbach, J.L., (1991) Microelectrodes: Theory and Applications, , NATO-ASI, Kluwer Academic Publishers, Amsterdam, The Netherlands; 
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Sousa, J.P., (1991), Ph. D. Thesis, University of UtahSousa, J.P., (1994) J. Electroanal. Chem., 372, p. 151; 
Sousa, J.P., Morais, S.B., Pereira, M.C., Pereira, M.L., Carvalho, G.S., Barbosa, M.A., (1994) Biomaterials, 15, p. 821; 
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Kovacs, P., Istephanous, N.S., (1994) Compatibility of Biomedical Implants, , The Electrochemical Society, Pennington, NJ; 
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Pereira, M.L., Pereira, M.C., Sousa, J.P., (1995) Biomed, Lett., 52, p. 235
},
	month = {1997///},
	pages = {150 - 154},
	abstract = {Mercury film microelectrodes (MFMs) were used to quantify trace levels of nickel accumulated in mice organs samples after dosing the mice with a physiological solution containing 150 ppm of Ni ions over a period of time, by performing adsorptive cathodic stripping square wave voltammetry (AdSWV). The residues obtained after digestion of the biological organs with nitric acid which affects the electrochemical response of Ni were investigated by XRD and IR spectrometry and found to be mainly composed by p-nitrobenzoic acid. Electrochemical conditions for the quantification of Niwith MFMs in the biological solutions were optimized. The electrochemical data compared favorably with that obtained by atomic absorption spectrometry.},
	keywords = {Adsorptive stripping voltammetry, Mice organs, Microelectrodes, Nickel},
	isbn = {10400397 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0000122410\&partnerID=40\&md5=634a3532a71c048970c98a2652cca21b},
	author = {Pereira, M.C. and Faria, J.L. and Reis, M.L. and Sousa, J.P.}
}
@article {2598,
	title = {Production, bonding strength and electrochemical behaviour of commercially pure Ti/Al                    2O
                    3 brazed joints
},
	journal = {Journal of Materials ScienceJ Mater Sci},
	volume = {32},
	year = {1997},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: JMTSALanguage of Original Document: EnglishCorrespondence Address: Paiva, O.C.; INEB-Inst. de Engenharia Biomedica, Pra{\c c}a do Coronel Pacheco 1, 4050 Porto, PortugalReferences: Paiva, O.C., Barbosa, M.A., (1995) Advances in Materials Science and Implant Orthopaedic Surgery, p. 275. , edited by R. Kossowsky and N. Kossowsky NATO ASI Series, Dordercht, The Netherlands;Hongqi, H., Zhihao, J., Xiaotian, W., (1994) J. Mater. Sci., 29, p. 5041; 
Santella, M.L., (1992) Ceram. Bull., 6, p. 947; 
Schwartz, M.M., (1990) Ceramic Joining, , ASM International, Ohio, USA; 
Nicholas, M., Mortimer, D., (1985) Mater. Sci. Technol., 1, p. 657; 
Kelkar, G.P., Carim, A.H., (1993) Ceramic Transactions - Structrual Ceramics Joining II, , edited by A. J. Moorhead, R. E. Loehman and S. M. Johnson American Ceramic Society, Ohio, USA; 
Li, J.-G., (1994) Ceram. Int., 20, p. 391; 
Mizuhara, H., Huebel, E., Oyama, T., (1989) Ceram. Bull., 9, p. 1591; 
Jackson, M.D., (1966) Welding Methods and Metallurgy, , Griffin Editions, London; 
Loehman, R.E., Tomsia, A.P., (1988) Ceram. Bull., 2, p. 375; 
Barbosa, M.A., Rocha, L., Pures, P., (1993) Monitoring of Orthopedic Implants - A Biomaterials/Microelectronics Challenge, p. 222. , edited by F. Burny and R. Pures Elsevier Science, North-Holland; 
Cassidy, R., Pence, R., Moddeman, W., (1989) Ceram. Eng. Sci. Proc., 10, p. 1582; 
Barbier, F., Peytour, C., Revcolevschi, A., (1990) J. Am. Ceram. Soc., 6, p. 1582; 
Loehman, R.E., Tomsia, A.P., (1992) Acta Metall. Mater., 40, pp. S75; 
Nicholas, M.G., Mortimore, D.A., (1985) Mater. Sci. Technol., 1, p. 657; 
Nicholas, M.G., (1986) Br. Ceram. Trans. J., 85, p. 144; 
Nicholas, M.G., Crispin, R.M., (1989) Ceram. Eng. Sci. Proc., 10, p. 1602; 
Misra, A.K., (1991) Metall. Trans., 22 A, p. 715; 
Stern, M., Geary, A.L., (1957) Corrosion, 13, p. 139; 
Barbosa, M.A., (1992) Biomaterials - Hard Tissue Repair and Replacement, p. 257. , edited by D. Muster Elsevier Science, North-Holland; 
Uhlig, H.H., (1967) Corrosion and Corrosion Control, , Wiley, New York; 
Shreir, L.L., (1978) Metal/Environment Reactions, 1. , Newnes-Butterworths, London
},
	month = {1997///},
	pages = {653 - 659},
	abstract = {The brazing of commercially pure titanium to Al                        2O
                        3 has been studied. Two different brazing alloys within the Ag-Cu-Ti system and pure silver were selected as bonding agents. Titanium hydride (TiH
                        2) additions were also tested, with the aim of improving the wetting of the ceramic surface by the melted brazing alloy. The mechanical and electrochemical behaviour of the produced joints was assessed, and related to chemical and morphological features resulting from an analysis by scanning electron microscopy and energy dispersive spectroscopy. It was possible to produce joints presenting high integrity, good strength and high resistance to corrosion. The best results were obtained when using an Ag-26Cu-3Ti brazing alloy. The addition of TiH
                        2 increased the mechanical properties, leading to a maximum bonding strength of 80 {\textpm} 8 MPa, as determined in three-point bending tests. In most of the cases, for a maximum deflection of 5 mm, there was only a partial detachment of the ceramic/metal joints. The lowest values for the corrosion rates (i
                        corr = 1.38 μA cm
},
	keywords = {Agents, Aluminum compounds, Aluminum trioxide, Bond strength (materials), Bonding, Brazed joints, Brazing, Brazing alloys, Corrosion resistance, Energy dispersive spectroscopy, Interfaces (materials), Joints (structural components), Scanning electron microscopy, Silver alloys, Strength of materials, Titanium, Titanium compounds, Titanium hydride},
	isbn = {00222461 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031075923\&partnerID=40\&md5=e1879111241c72f3096a0a8277f73c50},
	author = {Paiva, O.C. and Barbosa, M.A.}
}
@conference {2617,
	title = {Histological evaluation of two bioactive cements},
	booktitle = {Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials SymposiumTrans Annu Meet Soc Biomater Int Biomater Symp},
	series = {Proceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2)},
	volume = {2},
	year = {1996},
	note = {Conference code: 45651Export Date: 21 November 2012Source: ScopusCODEN: TAMSELanguage of Original Document: EnglishCorrespondence Address: Vasconcelos, M.; INEB-Inst Eng. Biomedica, Porto, Portugal},
	month = {1996///},
	pages = {61},
	publisher = {Soc for Biomaterials},
	organization = {Soc for Biomaterials},
	address = {Toronto, Can},
	abstract = {Two bioactive bone cements were compared with poly(methylmethacrylate) (PMMA) cement. Cement A was prepared from precipitated hydroxyapatite and tricalcium phosphate, using an aqueous solution of Na                        2HPO
                        4. Cement B was prepared by crushing a bioactive glass composed of SiO
                        2, CaO, P
                        2O
                        5 and Na
                        2O. The powder was mixed with NH
                        4H
                        2PO
                        4/(NH
                        4)
                        2 PO
                        4 aqueous solution buffered at pH = 7.4. The bioactive cements exhibited different in vivo behaviors but both performed better than PMMA cement.
},
	keywords = {Bioactive bone cements, bone cement, glass, hydroxyapatite, Ion exchange, Lime, pH, Phosphate minerals, Polymethyl methacrylates, Silica, Sodium compounds, Solutions, Sorption, Tricalcium phosphate},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030421685\&partnerID=40\&md5=14886fa9d05aee6f93ab00abbac772ad},
	author = {Vasconcelos, M. and Afonso, A. and Branco, R. and Planell, J. and Driessens, F. and Cavalheiro, J.},
	editor = {Anon}
}
@article {2628,
	title = {Implant recovery and tissue reaction in growing pigs following implantation of packaging materials for injectable electronic identification and monitoring devices},
	journal = {Preventive Veterinary MedicinePrev. Vet. Med.},
	volume = {25},
	year = {1996},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: PVMEELanguage of Original Document: EnglishCorrespondence Address: Janssens, S.; Lab. for Agric. Buildings Research, K.U. Leuven, K. Mercierlaan 92, B-3001 Heverlee, BelgiumReferences: Agresti, A., (1990) Categorical Data Analysis, , John Wiley, New York, 558 pp;Report from the working group on transponders (1990) CBSG News, 1 (3), p. 13; 
Davis, S.D., Gibbons, D.F., Martin, R.L., Levitt, S.R., Smith, J., Harrington, R.V., Biocompatibility of ceramic implants in soft tissue (1972) J. Biomed. Mater. Res., 6, pp. 425-449; 
Dorn, H.J., Vorstellung des EURO I.D. Systems zur kennzeichnung von tieren mit mikrochips in transpondern (1987) Tierarztl. Umschau, 12, pp. 978-981; 
Fallon, R.J., Rogers, P.A.M., Use and recovery of implantable electronic transponders in beef cattle (1991) Automatic Electronic Identification Systems for Farm Animals, pp. 61-67. , E. Lambooij (Editor), Commission European Community, Luxembourg; 
Konermann, H., Identification of horses by implantation of microchips (1991) Automatic Electronic Identification Systems for Farm Animals, pp. 37-43. , E. Lambooij (Editor), Commission European Community, Luxembourg; 
Lambooij, E., Injectable electronic identification systems: Some veterinary aspects (1991) Automatic Electronic Identification Systems for Farm Animals, pp. 21-27. , E. Lambooij (Editor), Commission European Community, Luxembourg; 
Lambooij, E., Merks, J.W.M., De techniek en plaats van injectie van elektronische levensnummers in varkens (1989) IVO-Rapp., B-335. , IVO-DLO, Zeist, The Netherlands, 12 pp; 
Lambooij, E., De Groot, P.H.S., Molenbeek, R.F., Gruys, E., Subcutaneous tissue reaction to polyethylene terephtalate-covered electronic identification transponders in pigs (1992) Vet. Q., 14 (4), pp. 145-147; 
Interim report following six month checks (1991) Livestock Identification Trial, , MLC, Milton Keynes, UK, 14 pp; 
Petersen, B., Kunneken, J., Norpoth, A., BIPS: An information and preventive system for pig breeding farms (1989) Pigs News Inf., 10 (4), pp. 473-476; 
Rao, G.N., Edmondson, J., Tissue reaction to an implantable identification device in mice (1990) Toxicol. Pathol., 18 (3), pp. 412-416; 
(1989) SAS/STAT User{\textquoteright}s Guide, Version 6, 4th Edn., 1. , SAS Institute Inc., Cary, NC, 943 pp; 
Williams, D.F., The response of the body environment to implants (1973) Implants in Surgery, pp. 203-297. , D.F. Williams (Editor), Saunders, London; 
Williams, D.F., Titanium and titanium alloys (1981) Biocompatibility of Clinical Implant Materials, 1, pp. 188-200. , D.F. Williams (Editor), CRC Press, Boca Raton, FL
},
	month = {1996///},
	pages = {249 - 258},
	abstract = {Different materials were used as cylindrical housings (40 mm {\texttimes} 5 mm) for implantable electronic identification and monitoring tags in livestock. A total of 299 pigs received an implant in the right ear base. Wound healing was complete after 6 weeks in all animals, whereas tissue reaction at the site of the transponder started from the first week with highest levels between 6 and 9 weeks. About 10\% of pigs showed visible swelling of the tissue surrounding the implant. Severeness of tissue reaction ante-mortem influenced the recovery rate of implants. Histological sections obtained from the pigs at slaughter show signs of an inflammatory response in most of the pigs which was associated with the implantation duration/body weight effect. Overall recovery of implants was 90\%. The unfavourable inflammation and recovery results might be attributed to a sub-optimal injection procedure.},
	keywords = {implant, Implant recovery, Pig, tissue reaction},
	isbn = {01675877 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038873896\&partnerID=40\&md5=5fb7280a2742ab9dfe152ec1045acb44},
	author = {Janssens, S. and Rocha, L.A. and Bosschaerts, L. and Barbosa, M.A. and Puers, R. and Vill{\'e}, H. and Geers, R.}
}
@article {2636,
	title = {Stainless steel corrosion products cause alterations on mouse spleen cellular populations},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {6},
	year = {1995},
	note = {Cited By (since 1996): 19Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00121249Language of Original Document: EnglishCorrespondence Address: Tracana, R.B.; Natl. Inst. Biomedical Engineering, INEB, Praca Coronel Pacheco 1, 4000 Porto, Portugal},
	month = {1995///},
	pages = {56 - 61},
	publisher = {Chapman \& Hall Ltd},
	abstract = {Stainless steel is a metallic biomaterial commonly used in orthopaedic surgery. In this study we looked at the effects of stainless steel corrosion products on spleen, in order to evaluate their potential immunotoxicological effects. For this purpose stainless steel, type AISI 316L, was electrochemically dissolved in a physiological salt solution. The final solution, containing 490 μg/ml Fe, 224 μg/ml Cr and 150 μg/ml Ni, was injected subcutaneously in mice. After several periods of time (4, 10 and 14 days) spleens were removed and analysed for: (a) their contents in Fe, Cr and Ni, (b) histological alterations, (c) enumeration of cellular populations. Results showed that stainless steel corrosion products accumulated in spleen, caused histological alterations, and induced changes in cellular populations. When compared to chromium and nickel, iron was the metal ion preferentially accumulated in spleen. The increase of the multinucleated giant cell population was accompanied by depletion of lymphocyte populations. This study indicates that stainless steel corrosion products can spread systemically and accumulate in spleen inducing histological and cellular alterations which may give rise to immunotoxicological consequences.Stainless steel is a metallic biomaterial commonly used in orthopaedic surgery. In this study we looked at the effects of stainless steel corrosion products on spleen, in order to evaluate their potential immunotoxicological effects. For this purpose stainless steel, type AISI 316L, was electrochemically dissolved in a physiological salt solution. The final solution, containing 490 μg/ml Fe, 224 μg/ml Cr and 150 μg/ml Ni, was injected subcutaneously in mice. After several periods of time (4, 10 and 14 days) spleens were removed and analysed for: (a) their contents in Fe, Cr and Ni, (b) histological alterations, (c) enumeration of cellular populations. Results showed that stainless steel corrosion products accumulated in spleen, caused histological alterations, and induced changes in cellular populations. When compared to chromium and nickel, iron was the metal ion preferentially accumulated in spleen. The increase of the multinucleated giant cell population was accompanied by depletion of lymphocyte populations. This study indicates that stainless steel corrosion products can spread systematically and accumulate in spleen inducing histological and cellular alterations which may give rise to immunotoxicological consequences.
},
	keywords = {animal cell, animal experiment, animal tissue, Animalia, article, Biocompatibility, biomaterial, cell population, Cells, Chromium, Chronoamperometry, controlled study, Corrosion, Corrosion products, Dissolution, electrochemistry, giant cell, histology, immunology, immunotoxicity, Immunotoxicological effects, Ions, Iron, Lymphocyte, lymphocyte subpopulation, male, mouse, Nickel, nonhuman, orthopedic surgery, priority journal, sodium chloride, Spleen, spleen cell, Stainless steel, Steel corrosion, subcutaneous drug administration, toxicity},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028883275\&partnerID=40\&md5=0d4737517419b9f7ccafa996ef5e1bb1},
	author = {Tracana, R.B. and Pereira, M.L. and Abreu, A.M. and Sousa, J.P. and Carvalho, G.S.}
}
