



@article {3321,
	title = {Natriuretic peptide system modulation in uninephrectomized rats},
	journal = {Experimental and Clinical Cardiology},
	volume = {20},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {2368 - 2387},
	abstract = {BACKGROUND: Cardiac activation of the natriuretic peptide (NP) system plays a major role in the renal adaptations to volume, expansion (VE). However, its role after uninephrectomy (Unx) remains to be elucidated. In the present study, we evaluated cardiac hemodynamics, plasma NP-Type B (BNP) levels nd NP receptor A (NPR-A) and C (NPR-C) expression in the renal cortex (RC) and medulla (RM) of Sham and Unx rats. METHODS: Male Wistar-Han rats (n=42) were randomly assigned to Unx or Sham surgery. Non-invasive blood pressure (BP), invasive left ventricular (LV) hemodynamics and natriuretic response to VE were performed and plasma was collected for BNP determination, 2, 10 and 26 weeks after surgery. In addition, samples from RC and RM were obtained for NPR-A and -C quantification (RT-PCR and western-blot). RESULTS: In Unx, blood pressure elevation and reduced natriuretic response to VE were observed. Although plasma BNP levels increased overtime, no LV dysfunction or myocardial BNP gene activation was observed in Unx. NPR-A mRNA in RC and RM were significantly increased in Unx at 26 weeks. This was accompanied by decreased NPR-C mRNA in the RM in Unx at 10 and 26 weeks, with no differences detected in the RC. CONCLUSIONS: In our experimentalmodel of Unx, progressive BP elevation and reduced natriuretic response to VE were accompanied by modulation of NPR-A and -C expression, in both the RC and RM, in the absence of cardiac activation of the NP system. Our results suggest a local renal modulation of the NP system after Unx. {\textcopyright} 2013 et al.; licensee Cardiology Academic Press.},
	keywords = {BNP, Hypertension, Natriuretic peptide receptor, Uninephrectomy},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905968553\&partnerID=40\&md5=54369af569474ee0bfddfa5aeac3237a},
	author = {Santos-Ara{\'u}jo, C. and Roncon-Albuquerque Jr., R. and Moreira-Rodrigues, M. and Henriques-Coelho, T. and Quelhas-Santos, J. and Faria, B. and Sampaio-Maia, B. and Leite-Moreira, A.F. and Pestana, M.}
}
@article {3313,
	title = {Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients},
	journal = {Experimental Biology and Medicine},
	volume = {239},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {502 - 508},
	abstract = {Renalase is a recently described enzyme secreted by the kidney into both plasma and urine, where it was suggested to degrade catecholamines contributing to blood pressure control. While there is a controversy regarding the relationship between renal function and plasma renalase levels, there is virtually no data in humans on plasma renalase activity as well as on both urine renalase levels and activity. We prospectively examined the time course of plasma and urine renalase levels and activity in 26 end-stage renal disease (ESRD) patients receiving a cadaver kidney transplant (cadaver kidney recipients [CKR]) before surgery and during the recovery of renal function up to day 90 post transplant. The relationship with sympathetic and renal dopaminergic activities was also evaluated. The recovery of renal function in CKR closely predicted decreases in plasma renalase levels (r = 0.88; P < 0.0001), urine renalase levels (r = 0.75; P < 0.0001) and urine renalase activity (r = 0.56; P < 0.03), but did not predict changes in plasma renalase activity (r = -0.02; NS). Plasma norepinephrine levels positively correlated with plasma renalase levels (r = 0.64, P < 0.002) as well as with urine renalase levels and activity (r = 0.47 P < 0.02; r = 0.71, P < 0.0005, respectively) and negatively correlated with plasma renalase activity (r = -0.57, P < 0.002). By contrast, plasma epinephrine levels positively correlated with plasma renalase activity (r = 0.67, P < 0.0001) and negatively correlated with plasma renalase levels (r = -0.62, P < 0.003). A significant negative relationship was observed between urine dopamine output and urine renalase levels (r = -0.48; P < 0.03) but not with urine renalase activity (r = -0.33, NS). We conclude that plasma and urine renalase levels closely depend on renal function and sympathetic nervous system activity. It is suggested that epinephrine-mediated activation of circulating renalase may occur in renal transplant recipients with good recovery of renal function. The increase in plasma renalase activity observed in ESRD patients and renal transplant recipients can be explained on the basis of reduced inhibition of the circulating enzyme. {\textcopyright} 2014 by the Society for Experimental Biology and Medicine.},
	keywords = {catecholamines, CKD, renal function, renal transplant, renalase},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84898964878\&partnerID=40\&md5=965164d696f11b12d4767bda11271186},
	author = {Quelhas-Santos, J. and Soares-Silva, I. and Fernandes-Cerqueira, C. and Sim{\~o}es-Silva, L. and Ferreira, I. and Carvalho, C. and Coentr{\~a}o, L. and Vaz, R. and Sampaio-Maia, B. and Pestana, M.}
}
@article {3416,
	title = {Plasma Renalase in Chronic Kidney Disease: Differences and Similarities between Humans and Rats},
	journal = {Current hypertension reviews},
	volume = {10},
	year = {2014},
	month = {2014},
	pages = {166-70},
	url = {<Go to ISI>://MEDLINE:25567503},
	author = {Quelhas-Santos, Janete and Pestana, Manuel}
}
@book {2201,
	title = {Automatic assessment of Leishmania infection indexes on in vitro macrophage cell cultures},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {7325 LNCS},
	year = {2012},
	note = {Conference code: 91199Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-31298-4_51Language of Original Document: EnglishCorrespondence Address: Leal, P.; Universidade Do Porto, Faculdade de Engenharia, Departamento de Engenharia Qu{\'\i}micaPortugalReferences: Castro, H., Sousa, C., Santos, M., Cordeiro-da-Silva, A., Floh{\'e}, L., Tom{\'a}s, A., Complementary antioxidant defence by cytoplasmic and mitochondrial peroxiredoxins in Leishmania infantum (2002) Free Radical Biology and Medicine, 33, pp. 1552-1562;Chen, X., Zhou, X., Wong, S., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Marcuzzo, M., Quelhas, P., Campilho, A., Maria Mendon{\c c}a, A., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Computers in Biology and Medicine, 39 (9); 
Chen, Y., Ladi, E., Herzmark, P., Robey, E., Roysam, B., Automated 5-d analysis of cell migration and interaction in the thymic cortex from time-lapse sequences of 3-d multi-channel multi-photon images (2009) J. Immunol. Methods, 340 (1), pp. 65-80; 
Esteves, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Gradient convergence filters and a phase congruency approach for in vivo cell nuclei detection Machine Vision and Applications, , in press; 
Schmitt, O., Hasse, M., Morphologic multiscale decomposition of connected regions with emphasis on cell clusters (2009) CVIU, 113 (2), pp. 188-201; 
Usaj, M., Torkar, D., Kanduser, M., Miklavcic, D., Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast image (2010) Journal of Microscopy, 241 (3), pp. 303-314; 
Sezgin, M., Sankur, B., Survey over image thresholding techniques and quantitative performance evaluation (2003) Journal of Electronic Imaging, 13 (1), pp. 146-165; 
Lowe, D., Distinctive Image Features from Scale-Invariant Keypoints (2004) International Journal of Computer Vision; 
Lindberg, T., Scale-space theory: A basic tool for analysing structures at different scales (1994) Journal of Applied Statistics, pp. 11-24; 
Yan, P., Zhou, X., Shah, M., Wong, S.T.C., Automatic segmentation of high-throughput RNAi fluorescent cellular images (2008) IEEE Trans. Inf. Technol. Biomed., 12 (1), pp. 109-117; 
CellNote: Plataforma para An?alise Assistida de Imagens, Bruno Afonso Da Cruz Lopes, , Master Thesis, Faculty of Sciences of the University of Porto
},
	month = {2012///},
	pages = {432 - 439},
	address = {Aveiro},
	abstract = {Evaluation of parasite infection indexes on in vitro cell cultures is a practice commonly employed by biomedical researchers to address biological questions or to test the efficacy of novel anti-parasitic compounds. In the particular case of Leishmania infantum, a unicellular parasite that parasitizes macrophages, infection indexes are usually determined either by visual inspection of cells directly under the microscope or by counting digital images using appropriate software. In either case assessment of infection indexes is time consuming, thus motivating the creation of automatic image analysis approaches that allow large scale studies of Leishmania-infected macrophage cultures. We propose a fully automated method for automatic evaluation of parasite infection indexes through the segmentation of individual macrophages nucleus and cytoplasm, as well as the segmentation and co-localization of the parasites in the image. To perform such analysis with robustness and increased performance we propose the use of local image filters tuned to the specific size of the objects to detect, in conjunction with image segmentation approaches. The objects size estimation is then improved through a learning feedback loop. Cytoplasm is detected by seeded watershed segmentation. Our approach obtains, for 86 images from 4 experiments, an average parasite infection index evaluation error of 2.3\%. {\textcopyright} 2012 Springer-Verlag.},
	keywords = {Automated methods, Automatic assessment, Automatic evaluation, Automatic image analysis, Cell culture, Cell nuclei detection, cell nucleus, Colocalization, Digital image, Enzyme inhibition, Feed-back loop, Image analysis, Image filters, Image segmentation, In-vitro, Leishmania, Leishmania infantum, Macrophage cells, Macrophage culture, Macrophages, microscopy image segmentation, Microscopy images, Parasite infection, Parasite-, Size estimation, Visual inspection, Watershed segmentation},
	isbn = {03029743 (ISSN); 9783642312977 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864131020\&partnerID=40\&md5=71523f4ff6de3e6ecc717c2fce28284a},
	author = {Leal, P. and Ferro, L. and Marques, M. and Rom{\~a}o, S. and Cruz, T. and Tom{\'a}, A.M. and Castro, H. and Quelhas, P.}
}
@conference {2192,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical ImagingIEEE Comput. Soc. Conf. Comput. Vis. Pattern Recogn.},
	series = {2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012},
	year = {2012},
	note = {Conference code: 91746Export Date: 21 November 2012Source: ScopusArt. No.: 6235546doi: 10.1109/ISBI.2012.6235546Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Lutgens, E., Daemen, M., Muinck, E., Chronic myocardial infarction in the mouse: Cardiac structural and functional changes (1998) Cardiovascular Research, 41, pp. 586-593; 
Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Nascimento, D., Valente, M., Esteves, T., Pina, M.F., Guedes, J.G., Freire, A., Quelhas, P., Pinto-Do, O.P., Miquant - Semi-automation of infarct size assessment in models of cardiac ischemic injury (2011) Plos One, 6 (9), pp. 1-8; 
Esteves, T., Valente, M., Nascimento, D., Pinto-Do, O.P., Quelhas, P., Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model (2011) Proceedings of IbPRIA, LNCS, 6669, pp. 151-158; 
Pfeffer, M., Pfeffer, J., Fishbein, M., Myocardial infarct size and ventricular function in rats (1979) Circulation Research, 44, pp. 503-512
Sponsors: The Institute of Electrical and Electronic Engineers; Engineering in Medicine and Biology Society; Signal Processing Society},
	month = {2012///},
	pages = {310 - 313},
	address = {Barcelona},
	abstract = {Experimental rodent models of induced ischemic injury have been extensively used in biomedical research to study molecular, cellular and histological alterations following myocar-dial infarction. These models are increasingly employed to assess the potential of newly developed therapies for functional restoration of the damaged heart. Such studies are based on myocardial infarction induction followed by different therapeutic interventions and subsequent analysis of the infarct size. This analysis is used to evaluate the extent to which such interventions meet recovery of the lost myocardial tissue. Infarct size is defined as the percentage of the left ventricle affected by coronary artery occlusion. The infarct size is traditionally estimated manually delineating the infarcted and normal tissue areas in the left ventricle of the excised heart. However, this is a time-consuming, arduous and prone to bias process. Herein, we developed an anatomic model, adapted through expectation maximization, which allows for fully automatic analysis of the data. Experimental validation is performed comparing the proposed approach with manual annotation. The results obtained through anatomical model adaptation were coherent with those manually obtained and the differences where never higher than 10\%. {\textcopyright} 2012 IEEE.},
	keywords = {Anatomic models, anatomical model, Anatomical models, Automatic analysis, Biomedical research, Coronary arteries, expectation maximization, Experimental validations, Image segmentation, Infarct size, Ischemic injuries, Left ventricles, Manual annotation, Maximum principle, Medical imaging, Murine model, Myocardial Infarction, Myocardial infarction size, Myocardial tissue, Normal tissue, Rodent models, Therapeutic intervention, Tissue},
	isbn = {19457928 (ISSN); 9781457718588 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=087bcb09e5d5319029623283cba34c34},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@conference {2817,
	title = {Automatic myocardial infarction size extraction in an experimental murine model using an anatomical model},
	booktitle = {Proceedings - International Symposium on Biomedical Imaging},
	year = {2012},
	note = {Export Date: 2 October 2013Source: ScopusArt. No.: 6235546},
	month = {2012///},
	pages = {310 - 313},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864832695\&partnerID=40\&md5=6716e8eb5d812d3e0ffe54d87369d746},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@article {2206,
	title = {Gradient convergence filters and a phase congruency approach for in vivo cell nuclei detection},
	journal = {Machine Vision and ApplicationsMach Vision Appl},
	volume = {23},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: MVAPEdoi: 10.1007/s00138-012-0407-7Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB-Instituto de Engenharia Biom{\'e}dica, Oporto, Portugal; email: quelhas@fe.up.ptReferences: Bleau, A., Leon, L.J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370. , DOI 10.1006/cviu.1999.0822;Bresson, X., Vandergheynst, P., Thiran, J., A priori information in image segmentation: Energy functional based on shape statistical model and image information (2003) Proceedings of International Conference on Image Processing, pp. 428-428. , ICIP; 
Bunyak, F., Palaniappan, K., Nath, S.K., Baskin, T.I., Dong, G., Quantitative cellmotility for in vitrowound healing using level setbased active contour tracking (2011) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 1-4; 
Byun, J., Verardo, M.R., Sumengen, B., Lewis, G.P., Manjunath, B.S., Fisher, S.K., Automated tool for the detection of cell nuclei in digital microscopic images: Application to retinal images (2006) Mol. Vis., 12, pp. 949-960; 
Campilho, A., Garcia, B., Toorn, H.V.D., Wijk, H.V., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48 (4), pp. 619-627. , DOI 10.1111/j.1365-313X.2006.02892.x; 
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Chen, Y., Ladi, E., Herzmark, P., Robey, E., Roysam, B., Automated 5-d analysis of cell migration and interaction in the thymic cortex from time-lapse sequences of 3-d multi-channel multi-photon images (2009) J. Immunol. Methods, 340 (1), pp. 65-80; 
Chen, Y., Quelhas, P., Campilho, A., Low frame rate cell tracking: A delaunay graph matching approach (2011) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 1-4; 
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Marcuzzo, M., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Tracking of Arabidopsis thaliana root cells in time-lapsemicroscopy (2009) Proceedings of the International Conference on Pattern Recognition (ICPR), pp. 1-4; 
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Quelhas, P., Marcuzzo, M., Mendonca, A.M., Campilho, A., Cell nuclei and cytoplasm joint segmentation using the sliding band filter (2010) IEEE Trans. Med. Imaging, 29 (8), pp. 1463-1473; 
Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., 3d cell nuclei fluorescence quantification using sliding band filter (2010) International Conference on Pattern Recognition (ICPR), pp. 2508-2511; 
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Yin, Z., Bise, R., Chen, M., Kanade, T., Cell segmentation in microscopy imagery using a bag of local Bayesian classifiers (2010) Proceedings of the IEEE International Conference on Biomedical Imaging (ISBI), pp. 125-128
},
	month = {2012///},
	pages = {623 - 638},
	abstract = {Computational methods used in microscopy cell image analysis have largely augmented the impact of imaging techniques, becoming fundamental for biological research. The understanding of cell regulation processes is very important in biology, and in particular confocal fluorescence imaging plays a relevant role for the in vivo observation of cells. However, most biology researchers still analyze cells by visual inspection alone, which is time consuming and prone to induce subjective bias. This makes automatic cell image analysis essential for large scale, objective studies of cells. While the classic approach for automatic cell analysis is to use image segmentation, for in vivo confocal fluorescence microscopy images of plants, such approach is neither trivial nor is it robust to image quality variations. To analyze plant cells in in vivo confocal fluorescence microscopy images with robustness and increased performance, we propose the use of local convergence filters (LCF). These filters are based in gradient convergence and as such can handle illumination variations, noise and low contrast.We apply a range of existing convergence filters for cell nuclei analysis of the Arabidopsis thaliana plant root tip. To further increase contrast invariance, we present an augmentation to local convergence approaches based on image phase information. Through the use of convergence index filters we improved the results for cell nuclei detection and shape estimation when compared with baseline approaches. Using phase congruency information we were able to further increase performance by 11\% for nuclei detection accuracy and 4\% for shape adaptation. Shape regularization was also applied, but with no significant gain, which indicates shape estimation was good for the applied filters. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Biological research, Cell analysis, Cell images, cell nucleus, Cell regulation, Cell segmentation, Cells, Confocal fluorescence imaging, Confocal fluorescence microscopy, Convergence filters, cytology, Detection accuracy, Estimation, Fluorescence microscopy, Illumination variation, Image analysis, Image filtering, Image segmentation, Imaging techniques, In-vivo, Information use, Local Convergence, Local image filtering, Medical applications, Phase congruency, Phase information, Plant cells, Plant roots, Plants (botany), Quality variation, Shape adaptation, Shape estimation, Shape regularization, Visual inspection},
	isbn = {09328092 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865374061\&partnerID=40\&md5=8e2b458028b5492266f085546a6804d9},
	author = {Esteves, T. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2241,
	title = {The Arabidopsis D-Type Cyclin CYCD2;1 and the Inhibitor ICK2/KRP2 Modulate Auxin-Induced Lateral Root Formation},
	journal = {Plant CellPlant Cell},
	volume = {23},
	year = {2011},
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},
	month = {2011///},
	pages = {641 - 660},
	abstract = {The integration of cell division in root growth and development requires mediation of developmental and physiological signals through regulation of cyclin-dependent kinase activity. Cells within the pericycle form de novo lateral root meristems, and D-type cyclins (CYCD), as regulators of the G                        1-to-S phase cell cycle transition, are anticipated to play a role. Here, we show that the D-type cyclin protein CYCD2;1 is nuclear in Arabidopsis thaliana root cells, with the highest concentration in apical and lateral meristems. Loss of CYCD2;1 has a marginal effect on unstimulated lateral root density, but CYCD2;1 is rate-limiting for the response to low levels of exogenous auxin. However, while CYCD2;1 expression requires sucrose, it does not respond to auxin. The protein Inhibitor-Interactor of CDK/Kip Related Protein2 (ICK2/KRP2), which interacts with CYCD2;1, inhibits lateral root formation, and ick2/krp2 mutants show increased lateral root density. ICK2/ KRP2 can modulate the nuclear levels of CYCD2;1, and since auxin reduces ICK2/KRP2 protein levels, it affects both activity and cellular distribution of CYCD2;1. Hence, as ICK2/KRP2 levels decrease, the increase in lateral root density depends on CYCD2;1, irrespective of ICK2/CYCD2;1 nuclear localization. We propose that ICK2/KRP2 restrains root ramification by maintaining CYCD2;1 inactive and that this modulates pericycle responses to auxin fluctuations. {\textcopyright} 2011 American Society of Plant Biologists.
},
	keywords = {Arabidopsis, Arabidopsis protein, Arabidopsis Proteins, Arabidopsis thaliana, article, ATP dependent 26S protease, cell cycle, cell cycle protein, Cell Cycle Proteins, cell nucleus, CycD2 protein, Arabidopsis, cycline, Cyclins, gene expression regulation, Gene Expression Regulation, Plant, genetics, growth, development and aging, ICK2 protein, Arabidopsis, indoleacetic acid derivative, Indoleacetic Acids, metabolism, mutation, plant root, Plant roots, proteasome, Proteasome Endopeptidase Complex},
	isbn = {10404651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953123085\&partnerID=40\&md5=f1355f64db2bcf9f2d881104f23005eb},
	author = {Sanz, L. and Dewitte, W. and Forzani, C. and Patell, F. and Nieuwland, J. and Wen, B. and Quelhas, P. and de Jager, S. and Titmus, C. and Campilho, A. and Ren, H. and Estelle, M. and Wang, H. and Murray, J.A.H.}
}
@book {2222,
	title = {Arabidopsis thaliana automatic cell file detection and cell length estimation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6754 LNCS},
	year = {2011},
	note = {Conference code: 85546Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21596-4_1Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB - Divis{\~a}o de Sinal e Imagem, Instituto de Engenharia Biom{\'e}dica, Porto, PortugalReferences: Terpstra, I., Heidstra, R., Stem cells: The root of all cells (2009) Semin. Cell Dev. Biol., 20 (9), pp. 1089-1096;Bennett, T., Scheres, B., Root development-two meristems for the price of one? (2010) Curr. Top Dev. Bio., 91, pp. 67-102; 
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Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Transactions on Systems, Man, and Cybernetics, SMC-9 (1), pp. 62-66
},
	month = {2011///},
	pages = {1 - 11},
	address = {Burnaby, BC},
	abstract = {In plant development biology, the study of the structure of the plant{\textquoteright}s root is fundamental for the understanding of the regulation and interrelationships of cell division and cellular differentiation. This is based on the high connection between cell length and progression of cell differentiation and the nuclear state. However, the need to analyse a large amount of images from many replicate roots to obtain reliable measurements motivates the development of automatic tools for root structure analysis. We present a novel automatic approach to detect cell files, the main structure in plant roots, and extract the length of the cells in those files. This approach is based on the detection of local cell file characteristic symmetry using a wavelet based image symmetry measure. The resulting detection enables the automatic extraction of important data on the plant development stage and of characteristics for individual cells. Furthermore, the approach presented reduces in more than 90\% the time required for the analysis of each root, improving the work of the biologist and allowing the increase of the amount of data to be analysed for each experimental condition. While our approach is fully automatic a user verification and editing stage is provided so that any existing errors may be corrected. Given five test images it was observed that user did not correct more than 20\% of all automatically detected structure, while taking no more than 10\% of manual analysis time to do so. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Automatic extraction, Automatic tools, Bio-imaging, BioImaging, cell differentiation, Cell divisions, Cell lengths, cell proliferation, Cells, Cellular differentiation, Experimental conditions, Image analysis, image symmetry, Individual cells, Main structure, Manual analysis, Nuclear state, Plant development, Plant roots, Plants (botany), Reliable measurement, Root structure, Test images, User verification, Wavelet analysis, Wavelet-based images},
	isbn = {03029743 (ISSN); 9783642215957 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960337749\&partnerID=40\&md5=ef49700082788511730e38d4eda9a566},
	author = {Quelhas, P. and Nieuwland, J. and Dewitte, W. and Mendon{\c c}a, A.M. and Murray, J. and Campilho, A.}
}
@book {2224,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6669 LNCS},
	year = {2011},
	note = {Conference code: 85429Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21257-4_19Language of Original Document: EnglishCorrespondence Address: Esteves, T.; Departamento de Engenharia Electrot{\'e}cnica e de Computadores, Faculdade de Engenharia, Universidade Do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: meb09026@fe.up.ptReferences: Degabriele, N., Griesenbach, U., Sato, K., Critical appraisal of the mouse model of myocardial infarction (2004) Experimental Physiology, 89 (4), pp. 497-505;Takagawa, J., Zhang, Y., Wong, M., Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches (2007) Journal of Applied Physiology, 102, pp. 2104-2111; 
Alattar, M., Osman, N., Fahmy, A., Myocardial segmentation using constrained multi-seeded region growing (2010) LNCS, 6112, pp. 89-98. , Campilho, A., Kamel, M. (eds.) ICIAR 2010 Springer, Heidelberg; 
Wu, Q., Merchant, F., Castleman, K., (1996) Microscope Image Processing, , ch. 7. Elsevier, Amsterdam; 
Gonzalez, R., Woods, R., Eddins, S., (2004) Digital Image Processing Using MATLAB, , ch. 9. Pearson Education, London; 
Sharma, N., Aggarwal, L., Automated medical image segmentation techniques (2010) Journal of Medical Physics, 35 (1), pp. 3-14; 
Pham, D., Xu, C., A survey of current methods in medical image segmentation (1998) Annual Review of Biomedical Engineering, 2, pp. 315-338; 
Hamarneh, G., Li, X., Watershed segmentation using prior shape and appearance knowledge (2009) Image and Vision Comp, 27 (1-2), pp. 59-68; 
Khadir, S., Ahamed, R., Moving toward region-based image segmentation techniques: A study (2009) Journal of Theoretical and Applied Information Technology, 5 (1), pp. 1-7; 
Ahmed, M., Mohamad, D., Segmentation of brain mr images for tumor extraction by combining kmeans clustering and perona-malik anisotropic diffusion model (2010) International Journal of Image Processing, 2 (1), pp. 1-8; 
Mayer, A., Greenspan, H., An adaptive mean-shift framework for MRI brain segmentation (2009) IEEE Trans. on Medical Imaging, 28 (8), pp. 1-12; 
Dabov, K., Foi, A., Katkovnik, V., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 1-16; 
Otsu, N., A threshold selection method from gray level histograms (1979) IEEE Trans. Systems, Man and Cybernetics, 9 (1), pp. 62-66; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Computers in Biology and Medicine, 39 (9), pp. 1-9; 
Bleau, A., Leon, J., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370
},
	month = {2011///},
	pages = {151 - 158},
	address = {Las Palmas de Gran Canaria},
	abstract = {Rodent models of myocardial infarction (MI) have been extensively used in biomedical research towards the implementation of novel regenerative therapies. Permanent ligation of the left anterior descending (LAD) coronary artery is a commonly used method for inducing MI both in rat and mouse. Post-mortem evaluation of the heart, particularly the MI extension assessment performed on histological sections, is a critical parameter for this experimental setting. MI extension, which is defined as the percentage of the left ventricle affected by the coronary occlusion, has to be estimated by identifying the infarcted- and the normal-tissue in each section. However, because it is a manual procedure it is time-consuming, arduous and prone to bias. Herein, we introduce semi-automatic and automatic approaches to perform segmentation which is then used to obtain the infarct extension measurement. Experimental validation is performed comparing the proposed approaches with manual annotation and a total error not exceeding 8\% is reported in all cases. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Extension evaluations, Image analysis, Image segmentation, Infarct extension evaluation, k-means, Mammals, meanshift, otsu, Pattern recognition, region growing, Tissue, watershed},
	isbn = {03029743 (ISSN); 9783642212567 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=360bb21a8c1c3a5fdd394c647fcadbbc},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@book {2818,
	title = {Automatic and semi-automatic analysis of the extension of myocardial infarction in an experimental murine model},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)},
	volume = {6669 LNCS},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: Scopus},
	month = {2011///},
	pages = {151 - 158},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959927807\&partnerID=40\&md5=58726bd7fc58cf587a13af9e3ba0c151},
	author = {Esteves, T. and Valente, M. and Nascimento, D.S. and Pinto-Do-{\'O}, P. and Quelhas, P.}
}
@conference {2219,
	title = {Low frame rate cell tracking: A Delaunay graph matching approach},
	booktitle = {Proceedings - International Symposium on Biomedical ImagingIEEE Comput. Soc. Conf. Comput. Vis. Pattern Recogn.},
	series = {2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI{\textquoteright}11},
	year = {2011},
	note = {Conference code: 87101Export Date: 21 November 2012Source: ScopusArt. No.: 5872573doi: 10.1109/ISBI.2011.5872573Language of Original Document: EnglishCorrespondence Address: Chen, Y.; INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre, 823, 4150-180 Porto, PortugalReferences: Godinez, W.J., Lampe, M., Worz, S., Muller, B., Eils, R., Rohr, K., Tracking of virus particles in time-lapse fluorescence microscopy image sequences (2007) Proceedings of the IEEE International Symposium on Biomedical Imaging, pp. 256-259;Finch, A.W., Wilson, R.C., Hancock, E.R., Symbolic graph matching with the em algorithm (1998) Pattern Recognit, 31 (11), pp. 1777-1790. , Nov; 
Finch, M.A., Wilson, C.R., Hancock, R.E., Matching delaunay graphs (1997) Pattern Recognit, 30 (1), pp. 123-140. , Nov; 
Quelhas, P., Marcuzzo, M., Mendon{\c c}a, A.M., Campilho, A., Cell nuclei and cytoplasm joint segmentation using the sliding band filter (2010) IEEE Transactions on Medical Imaging, 29, pp. 1463-1473; 
Fortune, S., A sweepline algorithm for voronoi diagram (1987) Algorithmica, 2, pp. 153-174; 
Khoo, K., Suganthan, P., Evaluation of genetic operators and solution representations for shape recognition by genetic algorithms (2002) Patt. Recognit. Lett., 23 (13), pp. 1589-1597; 
Caetano, T.S., Caelli, T., Schuurmans, D., Barone, D., Graphical models and point pattern matching (2006) IEEE Trans. on Pattern Analysis and Machine Intelligence, 28 (10), pp. 1646-1662. , Oct; 
Raveaux, R., Burie, J.-C., Ogier, J.-M., A graph matching method and a graph matching distance based on subgraph assignments (2010) Pattern Recognition Letters, 31, pp. 394-406; 
Marcuzzo, M., Quelhas, P., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) IbPRIA 2009, 5524, pp. 168-175. , LNCS
Sponsors: Inst. Electr. Electron. Eng., Eng. Med. Biol. Soc. (IEEE EMBS); Inst. Electr. Electron. Eng., Signal Process. Soc. (IEEE SPS)},
	month = {2011///},
	pages = {1015 - 1018},
	address = {Chicago, IL},
	abstract = {Cell tracking is a fundamental problem for studying live cell dynamics. A novel approach for low frame rate cell tracking in fluorescent microscopy image is herein proposed. The method is based on sliding band filter detection and Delaunay triangulation sub-graph matching. With this approach we can track a large amount of small cells without their motion model and not relying on cell motion continuity between consecutive images. The effectiveness and robustness of the method were validated by visual inspection and on a ground truth dataset. {\textcopyright} 2011 IEEE.},
	keywords = {Band filters, cell motion, Cell tracking, Cells, Consecutive images, Data sets, Delaunay graph, Delaunay graph matching, Delaunay triangulation, Fluorescent microscopy, Frame rate, Fundamental problem, Ground truth, Live cell, low frame rate tracking, Medical imaging, Motion models, small target tracking, Small targets, Target tracking, Visual inspection},
	isbn = {19457928 (ISSN); 9781424441280 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80055027991\&partnerID=40\&md5=f347799ea22097a5550939fa287cd4ab},
	author = {Chen, Y. and Quelhas, P. and Campilho, A.}
}
@article {2815,
	title = {MIQuant - semi-automation of infarct size assessment in models of cardiac ischemic injury},
	journal = {PLoS ONE},
	volume = {6},
	year = {2011},
	note = {Cited By (since 1996):1Export Date: 2 October 2013Source: ScopusArt. No.: e25045},
	month = {2011///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80053583980\&partnerID=40\&md5=757f7f25dcffa46f5fd66ccee74f13f0},
	author = {Nascimento, D.S. and Valente, M. and Esteves, T. and De F{\'a}tima De Pina, M. and Guedes, J.G. and Freire, A. and Quelhas, P. and Pinto-Do-{\'O}, P.}
}
@article {2267,
	title = {Cell nuclei and cytoplasm joint segmentation using the sliding band filter},
	journal = {IEEE Transactions on Medical ImagingIEEE Trans. Med. Imaging},
	volume = {29},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusArt. No.: 5477157CODEN: ITMIDdoi: 10.1109/TMI.2010.2048253Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; Instituto de Engenharia Biom{\'e}dica (INEB), 4200-465 Porto, Portugal; email: pedro.quelhas@gmail.comChemicals/CAS: DNA, 9007-49-2; Actins; DNA, 9007-49-2References: Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Understand., 77 (3), pp. 317-370;Clocksin, W., Automatic segmentation of overlapping nuclei with high background variation using robust estimation and flexible contour models (2003) Proc. 12th Int. Conf. Image Anal. Process., pp. 682-687; 
Cloppet, F., Boucher, A., Segmentation of overlapping/aggregating nuclei cells in biological images (2008) Proc. 19th Int. Conf. Pattern Recognit. (ICPR 2008), pp. 1-4; 
Danek, O., Matula, P., De Solorzano, C.O., Munoz-Barrutia, A., Maska, M., Kozubek, M., Segmentation of touching cell nuclei using a two-stage graph cut model (2009) LNCS, 5575, pp. 410-419. , Proc. SCIA; 
Fok, Y., Chan, J., Chin, R., Automated analysis of nerve-cell images using active contour models (1996) IEEE Trans. Med. Imag., 15 (3), pp. 353-368. , Jun; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3-D fluorescence microscopy image sequences (2008) Workshop Bio-Image Inf.: Biol. Imag., Comput. Vis. Data Mining, pp. 17-18. , Santa Barbara, CA; 
Hill, A., Lapan, P., Li, Y., Haney, S., Impact of image segmentation on high-content screening data quality for sk-br-3 cells (2007) BMC Bioin-formatics, 8 (340), pp. 1-13; 
Hu, M., Ping, X., Ding, Y., A new active contour model and its application on cell segmentation (2004) 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV), 2, pp. 1104-1107. , TuA7.09 - P0673, 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV); 
Jones, T.R., Carpenter, A., Golland, P., Voronoi-based segmentation of cells on image manifolds (2005) ICCV Workshop Comput. Vis. Biomed. Image Appl. (CVBIA), pp. 535-543; 
Klemencic, A., Kovacic, S., Pernus, F., Automated segmentation of muscle fiber images using active contour models (1998) Cytometry, 32, pp. 317-326; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. Image Process., 8 (8), pp. 1029-1038. , Aug; 
Lehmussola, A., Ruusuvuori, P., Selinummi, J., Huttunen, H., Yli-Harja, O., Computational framework for simulating fluorescence microscope images with cell populations (2007) IEEE Trans. Med. Imag., 26 (7), pp. 1010-1016. , Jul; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. Pattern Anal. Mach. Intell., 13 (6), pp. 583-598. , Jun; 
Marcuzzo, M., Guichard, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Proceedings of IbPRIA. New York: Springer; 
Marcuzzo, M., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images (2009) LNCS, 5627, pp. 824-831. , Proceedings of ICIAR. New York: Springer; 
Moore, P., Molloy, D., A survey of computer-based deformable models (2007) Int. Mach. Vis. Image Process. Conf. (IMVIP), pp. 55-66; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Tran. Syst., Man, Cybern., 9 (1), pp. 62-66. , Jan; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A., Detection of lung nodule candidates in chest radiographs (2007) LNCS, 4478, pp. 170-177; 
Pereira, C.S., Mendon{\c c}a, A.M., Campilho, A., Evaluation of contrast enhancement filters for lung nodule detection Proceedings of ICIAR. New York: Springer, 4633, pp. 878-888; 
Quelhas, P., Marcuzzo, M., Oliveira, M.J., Mendon{\c c}a, A.M., Campilho, A., Cancer cell detection and invasion depth estimation in brightfield images (2009) Proc. 20th Br. Mach. Vis. Conf. (BMVC), , London, U.K; 
Ruusuvuori, P., Lehmussola, A., Selinummi, J., Rajala, T., Huttunen, H., Yli-Harja, O., Benchmark set of synthetic images for validating cell image analysis algorithms (2008) Proc. 16th Eur. Signal Process. Conf., , Lausanne, Switzerland; 
Solorzano, C., Malladi, R., Lelievre, S., Lockett, S., Segmentation of nuclei and cells using membrane related protein markers (2001) J. Microscopy, 201, pp. 404-415; 
Tek, F.B., Dempster, A.G., Kale, I., (2006) Mathematical Morphology:40 Years on, 30, pp. 441-454. , New York: Springer; 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proc. Int. Conf. Image Anal. Process., pp. 757-761; 
Xiong, G., Zhou, X., Ji, L., Bradley, P., Perrimon, N., Wong, S., Segmentation of drosophila RNAI fluorescence images using level sets (2006) Proc. IEEE Int. Conf. Image Process., pp. 73-76; 
Xiong, G., Zhou, X., Ji, L., Automated segmentation of drosophila RNAi fluorescence cellular images using deformable models (2006) IEEE Trans. Circ. Syst. i, 53 (11), pp. 2415-2424. , Nov; 
Yan, P., Zhou, X., Shah, M., Wong, S.T.C., Automatic segmentation of high-throughput RNAi fluorescent cellular images (2008) IEEE Trans. Inf. Technol. Biomed., 12 (1), pp. 109-117. , Jan; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. Circuits Syst., 53 (11), pp. 2405-2414. , Nov
},
	month = {2010///},
	pages = {1463 - 1473},
	abstract = {Microscopy cell image analysis is a fundamental tool for biological research. In particular, multivariate fluorescence microscopy is used to observe different aspects of cells in cultures. It is still common practice to perform analysis tasks by visual inspection of individual cells which is time consuming, exhausting and prone to induce subjective bias. This makes automatic cell image analysis essential for large scale, objective studies of cell cultures. Traditionally the task of automatic cell analysis is approached through the use of image segmentation methods for extraction of cells{\textquoteright} locations and shapes. Image segmentation, although fundamental, is neither an easy task in computer vision nor is it robust to image quality changes. This makes image segmentation for cell detection semi-automated requiring frequent tuning of parameters. We introduce a new approach for cell detection and shape estimation in multivariate images based on the sliding band filter (SBF). This filter{\textquoteright}s design makes it adequate to detect overall convex shapes and as such it performs well for cell detection. Furthermore, the parameters involved are intuitive as they are directly related to the expected cell size. Using the SBF filter we detect cells{\textquoteright} nucleus and cytoplasm location and shapes. Based on the assumption that each cell has the same approximate shape center in both nuclei and cytoplasm fluorescence channels, we guide cytoplasm shape estimation by the nuclear detections improving performance and reducing errors. Then we validate cell detection by gathering evidence from nuclei and cytoplasm channels. Additionally, we include overlap correction and shape regularization steps which further improve the estimated cell shapes. The approach is evaluated using two datasets with different types of data: a 20 images benchmark set of simulated cell culture images, containing 1000 simulated cells; a 16 images Drosophila melanogaster Kc167 dataset containing 1255 cells, stained for DNA and actin. Both image datasets present a difficult problem due to the high variability of cell shapes and frequent cluster overlap between cells. On the Drosophila dataset our approach achieved a precision/recall of 95\%/69\% and 82\%/90\% for nuclei and cytoplasm detection respectively and an overall accuracy of 76\%. {\textcopyright} 2010 IEEE.},
	keywords = {actin, Actins, animal, Animals, article, Band filters, Biological research, cell aggregation, Cell analysis, Cell culture, Cell detection, Cell images, cell nucleus, Cell segmentation, Cell Shape, Cell shapes, Cell size, Cells, chemistry, Computer vision, Convergence filters, Convex shapes, cytoplasm, Data sets, Databases, Factual, Digital image storage, DNA, Drosophila melanogaster, factual database, Fluorescence, Fluorescence microscopy, Fundamental tools, High variability, Image analysis, Image datasets, image processing, image processing in biology, Image Processing, Computer-Assisted, Image quality, Image segmentation, Imaging systems, Improving performance, Individual cells, Joint segmentation, Location, methodology, Microscopy, Fluorescence, Multivariate analysis, multivariate image processing, New approaches, Nuclear detection, Overlap correction, reproducibility, Reproducibility of Results, Semi-automated, Shape estimation, Shape regularization, ultrastructure, Visual communication, Visual inspection},
	isbn = {02780062 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955339493\&partnerID=40\&md5=f8cea406f7b2fcf901dc34fa940522b1},
	author = {Quelhas, P. and Marcuzzo, M. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2263,
	title = {Optical flow based Arabidopsis thaliana root meristem cell division detection},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6112 LNCS},
	year = {2010},
	note = {Conference code: 81097Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-13775-4_22Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Campilho, A., Garcia, B., Toorn, H.V.D., Wijk, H.V., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48 (4), pp. 619-627. , DOI 10.1111/j.1365-313X.2006.02892.x;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res., 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A., Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo (2007) Motion{\textquoteright}07, p. 13; 
Beemster, G.T.S., Baskin, T.I., Analysis of Cell Division and Elongation Underlying the Developmental Acceleration of Root Growth in Arabidopsis thaliana (1998) Plant Physiology, 116 (4), pp. 1515-1526; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Rohr, K., Automated analysis of mitotic cell nuclei in 3rd fluorescence microscopy image sequences Workshop on Bio-Image Inf.: Bio. Imag., Comp. Vision and Data Mining (2008); 
Yang, F., Mackey, M.A., Ianzini, F., Gallardo, G., Sonka, M., Cell segmentation, tracking, and mitosis detection using temporal context (2005) Medical Image Computing and Computer-Assisted Intervention (MICCAI), pp. 302-309; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Comp. in Biology and Medicine, 39, pp. 785-793; 
Marcuzzo, M., Guichard, T., Quelhas, P., Maria Mendon{\c c}a, A., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Araujo, H., Mendon{\c c}a, A.M., Pinho, A.J., Torres, M.I. (eds.) IbPRIA 2009. Springer, Heidelberg; 
Pereira, C.S., Mendon{\c c}a, A.M., Campilho, A., Evaluation of contrast enhancement filters for lung nodule detection (2007) LNCS, 4633, pp. 878-888. , Kamel, M.S., Campilho, A. (eds.) ICIAR 2007. Springer, Heidelberg; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of arabidopsis thaliana root meristem in confocal microscopy (2004) LNCS, 3212, pp. 166-174. , Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. Springer, Heidelberg; 
Hartley, R., Zisserman, A., (2003) Multiple View Geometry in Computer Vision, , Cambridge University Press, Cambridge; 
Lowe, D.G., Distinctive image features from scale-invariant keypoints (2004) International Journal of Computer Vision, 60, pp. 91-110; 
Brox, T., Bruhn, A., Papenberg, N., Weickert, J., High accuracy optical flow estimation based on a theory for warping (2004) LNCS, 3024, pp. 25-36. , Pajdla, T., Matas, J(G.) (eds.) ECCV 2004. Springer, Heidelberg
},
	month = {2010///},
	pages = {217 - 226},
	address = {Povoa de Varzim},
	abstract = {The study of cell division and growth is a fundamental aspect of plant biology research. In this research the Arabidopsis thaliana plant is the most widely studied model plant and research is based on in vivo observation of plant cell development, by time-lapse confocal microscopy. The research herein described is based on a large amount of image data, which must be analyzed to determine meaningful transformation of the cells in the plants. Most approaches for cell division detection are based on the morphological analysis of the cells{\textquoteright} segmentation. However, cells are difficult to segment due to bad image quality in the in vivo images. We describe an approach to automatically search for cell division in the Arabidopsis thaliana root meristem using image registration and optical flow. This approach is based on the difference of speeds of the cell division and growth processes (cell division being a much faster process). With this approach, we can achieve a detection accuracy of 96.4\%. {\textcopyright} 2010 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Biology, Biology image processing, cell division detection, Cell divisions, cell proliferation, confocal microscopy, Detection accuracy, Growth process, Image data, Image quality, Image segmentation, Imaging systems, In-vivo, In-vivo images, Metadata, Morphological analysis, Optical data processing, Optical flows, Plant biology, Plant cells, research, Root meristem},
	isbn = {03029743 (ISSN); 3642137741 (ISBN); 9783642137747 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955346994\&partnerID=40\&md5=22ef87a91388dfe96acf9e4ac6ad4377},
	author = {Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2300,
	title = {Automated Arabidopsis plant root cell segmentation based on SVM classification and region merging},
	journal = {Computers in Biology and MedicineComput. Biol. Med.},
	volume = {39},
	year = {2009},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: CBMDAdoi: 10.1016/j.compbiomed.2009.06.008PubMed ID: 19604506Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUP, Portugal; email: marcuzzo@fe.up.ptReferences: Baeza-Yates, R., Ribeiro-Neto, B., (1999) Modern Information Retrieval, , ACM Press;Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370; 
M. Brown, W. Grundy, L.D.N. Cristianini, C. Sugnet, T. Furey, M. Ares, D. Haussler, Knowledge-based analysis of microarray gene expression data using support vector machines, in: Proceedings of the National Academy of Sciences, 97 (1), 1999, pp. 262-267Burges, C., A tutorial on support vector machines for pattern recognition (1998) Data Mining and Knowledge Discovery, 2 (2), pp. 121-167; 
Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48, pp. 619-627; 
Chang, C., Lin, C., (2001) Libsvm: A library for support vector machines, , http://www.csie.ntu.edu.tw/\~{}cjlin/libsvm/faq.html$\#$f203, Software available online at; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Transactions on Biomedical Engineering, 53 (4), pp. 762-766; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Transactions on Image Processing, 16 (8), pp. 2080-2095; 
(2004) Kernel Methods in Computational Biology, Computational Molecular Biology, , B. Sch{\"o}lkopf, K. Tsuda, J.-P. Vert, Eds, MIT Press, Cambridge, MA; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of Arabidopsis thaliana root meristem in confocal microscopy (2004) Springer Lecture Notes in Computer Science, 3212, pp. 166-174. , Proceedings of the International Conference on Image Analysis and Recognition, Springer, Berlin; 
Grandjean, O., Vernoux, T., Laufs, P., Belcram, K., Mizukami, Y., Traas, J., In vivo analysis of cell division, cell growth, and differentiation at the shoot apical meristem in Arabidopsis (2004) Plant Cell, 16 (1), pp. 74-87; 
Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in Arabidopsis thaliana provided by a simple mathematical model (2006) Journal of Plant Research, 119, pp. 85-93; 
Kittler, J., Hatef, M., Duin, R.P.W., Matas, J., On combining classifiers (1998) IEEE Transactions on Pattern Analysis and Machine Intelligence, 20 (3), pp. 226-239; 
P. Kovesi, Symmetry and asymmetry from local phase, in: Proceedings of the 10th Australian Joint Conference on Artificial Intelligence, December 1997, pp. 185-190P. Kovesi, Edges are not just steps, in: Proceedings of the Fifth Asian Conference on Computer Vision, January 2002, pp. 822-827Liao, L., Noble, W.S., Combining pairwise sequence similarity and support vector machines for remote protein homology detection (2002) Proceedings of the Sixth Annual International Conference on Computational Molecular Biology, pp. 225-232. , ACM Press; 
Luc, V., Soille, P., Watersheds in digital spaces: an efficient algorithm based on immersion simulations (1991) IEEE Transactions on Pattern Analysis and Machine Intelligence, 13 (6), pp. 583-598; 
Mao, K., Zhao, P., Tan, P.-H., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Transactions on Biomedical Engineering, 53 (6), pp. 1153-1163; 
M. Marcuzzo, P. Quelhas, A. Campilho, A.M. Mendon{\c c}a, A.C. Campilho, Automatic cell segmentation from confocal microscopy images of the Arabidopsis root, in: IEEE International Symposium on Biomedical Imaging, May 2008, pp. 712-715Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A.C., A hybrid approach for cell image segmentation (2008) Springer Lecture Notes in Computer Science, 5112, pp. 739-749. , Proceedings of the International Conference on Image Analysis and Recognition, Springer, Berlin; 
Meyer, F., Beucher, S., Morphological segmentation (1990) Journal of Visual Communication and Image Representation, 1 (1), pp. 21-46; 
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T. Roberts, S. Mckenna, N. Wuyts, T. Valentine, A. Bengough, Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo, in: IEEE Workshop on Motion \& Video Computing, February 2007, pp. 13-21Sathiya Keerthi, S., Lin, C.-J., Asymptotic behaviors of support vector machines with Gaussian kernel (2003) Neural Computation, 15 (7), pp. 1667-1689; 
Vapnik, V., (1998) Statistical Learning Theory, , Wiley, New York
},
	month = {2009///},
	pages = {785 - 793},
	abstract = {To obtain development information of individual plant cells, it is necessary to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Automation of cell detection/marking process is important to provide research tools in order to ease the search for special events, such as cell division. In this paper we discuss an automatic cell detection approach for Arabidopsis thaliana based on segmentation, which selects the best cell candidates from a starting watershed-based image segmentation and improves the result by merging adjacent regions. The selection of individual cells is obtained using a support vector machine (SVM) classifier, based on a cell descriptor constructed from the shape and edge strength of the cells{\textquoteright} contour. In addition we proposed a novel cell merging criterion based on edge strength along the line that connects adjacent cells{\textquoteright} centroids, which is a valuable tool in the reduction of cell over-segmentation. The result is largely pruned of badly segmented and over-segmented cells, thus facilitating the study of cells. When comparing the results after merging with the basic watershed segmentation, we obtain 1.5\% better coverage (increase in F-measure) and up to 27\% better precision in correct cell segmentation. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.},
	keywords = {accuracy, Arabidopsis, Arabidopsis plant, Arabidopsis thaliana, article, artificial intelligence, automation, Bioinformatics, Biological cells, Biomedical image processing, Cell detection, Cell divisions, Cell membranes, Cell merging, cell proliferation, Cell segmentation, Cell Shape, Cell Wall, Computer simulation, confocal microscopy, controlled study, Descriptor, F-measure, Image analysis, image processing, Image Processing, Computer-Assisted, Image segmentation, Imaging systems, In-Vivo imaging, Individual cells, Landforms, Merging, Models, Biological, nonhuman, Over segmentation, Plant cells, plant root, Plant roots, Plants (botany), priority journal, Region-merging, Research tools, root morphology, Segmented cell, signal noise ratio, support vector machine, Support vector machines, SVM classification, Watershed segmentation, Watersheds},
	isbn = {00104825 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67849119398\&partnerID=40\&md5=8029bce67aff136ccc5e0f7e4f9e0ded},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Maria Mendon{\c c}a, A. and Campilho, A.}
}
@book {2302,
	title = {Cell division detection on the arabidopsis thaliana root},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5524 LNCS},
	year = {2009},
	note = {Conference code: 77027Cited By (since 1996): 5Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02172-5_23Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, p. 13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. on Circuits and Systems I-Regul. Papers, 53 (11), pp. 2405-2414; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3d fluorescence microscopy image sequences (2008) Workshop on Bio-Image Informatics: Biological Imaging, Computer Vision and Data Mining; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proceedings of the Int. Conference on Image Analysis and Processing, pp. 757-761; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A.C.: Detection of lung nodule candidates in chest radiographs. In: Mart{\'\i}, J., Bened{\'\i}, J.M., Mendon{\c c}a, A.M., Serrat, J. (eds.) IbPRIA 2007. LNCS, 4478, pp. 170-177. Springer, Heidelberg (2007)Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., A hybrid approach for cell image segmentation (2008) LNCS, 5112, pp. 739-749. , Campilho, A, Kamel, M.S, eds, ICIAR 2008, Springer, Heidelberg
Sponsors: International Association for Pattern Recognition (IAPR); Fundacao para a Ciencia e a Tecnologia (FCT)},
	month = {2009///},
	pages = {168 - 175},
	address = {Povoa de Varzim},
	abstract = {The study of individual plant cells and their growth structure is an important focus of research in plant genetics. To obtain development information at cellular level, researchers need to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Within this research field it is important to understand mechanisms like cell division and elongation of developing cells. We describe a tool to automatically search for cell division in the Arabidopsis thaliana using information of nuclei shape. The nuclei detection is based on a convergence index filter. Cell division detection is performed by an automatic classifier, trained through cross-validation. The results are further improved by a stability criterion based on the Mahalanobis distance of the shape of the nuclei through time. With this approach, we can achieve a correct detection rate of 94.7\%. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {A-stability, Arabidopsis thaliana, Automatic classifiers, Bioinformatics, Cell divisions, Cell membranes, cell proliferation, Cellular levels, confocal microscopy, Cross validation, Detection rates, Growth structures, Image analysis, In-plants, In-Vivo imaging, Mahalanobis distances, Pattern recognition, Plant cells, research, Research fields, Stability criteria},
	isbn = {03029743 (ISSN); 3642021719 (ISBN); 9783642021718 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68749115481\&partnerID=40\&md5=2c491950d3b00a9dd873aeaefdf5edcb},
	author = {Marcuzzo, M. and Guichard, T. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2312,
	title = {Contextual classification of image patches with latent aspect models},
	journal = {Eurasip Journal on Image and Video ProcessingEurasip J. Image Video Process.},
	volume = {2009},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusArt. No.: 602920doi: 10.1155/2009/602920Language of Original Document: EnglishCorrespondence Address: Monay, F.; Idiap Research Institute Martigny, 1920 Martigny, Switzerland; email: florent.monay@idiap.chReferences: Kumar, S., Hebert, M., Discriminative random fields: A discriminative framework for contextual interaction in classification, 2, pp. 1150-1157. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France;Lazebnik, S., Schmid, C., Ponce, J., Affine-invariant local descriptors and neighborhood statistics for texture recognition, 1, pp. 649-655. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Vogel, J., Schiele, B., Natural scene retrieval based on a semantic modeling step, 3115, pp. 207-215. , Proceedings of the 3rd International Conference on Image and Video Retrieval (CIVR 04) July 2004 Dublin, Ireland Lecture Notes in Computer Science; 
Shotton, J., Winn, J., Rother, C., Criminisi, A., TextonBoost: Joint appearance, shape and context modeling for multi-class object recognition and segmentation, 3951, pp. 1-15. , Proceedings of the 9th European Conference on Computer Vision (ECCV 06) May 2006 Graz, Austria Lecture Notes in Computer Science; 
Lowe, D.G., Distinctive image features from scale-invariant keypoints (2004) International Journal of Computer Vision, 60 (2), pp. 91-110; 
Dorko, G., Schmid, C., Selection of scale-invariant parts for object class recognition, 1, pp. 634-640. , Proceedings of the 9th IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Fei-Fei, L., Perona, P., A Bayesian hierarchical model for learning natural scene categories, 2, pp. 524-531. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 05) June 2005 San Diego, Calif, USA; 
Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., Van Gool, L., Modeling scenes with local descriptors and latent aspects, 1, pp. 883-890. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Sivic, J., Russell, B.C., Efros, A.A., Zisserman, A., Freeman, W.T., Discovering object categories in image collections, , Proceedings of the 10th IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Li, S.Z., (1995) Markov Random Field Modeling in Computer Vision, , New York, NY, USA Springer; 
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Murphy, K., Torralba, A., Freeman, W., Using the forest to see the trees: A graphical model relating features, objects and scenes, , Proceedings of the Neural Information Processing Systems December 2003 Vancouver, Canada; 
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Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., A thousand words in a scene (2007) IEEE Transactions on Pattern Analysis and Machine Intelligence, 29 (9), pp. 1575-1589; 
Quelhas, P., Odobez, J.-M., Multi-level local descriptor quantization for bag-of-visterms image representation, pp. 242-249. , Proceedings of the 6th ACM International Conference on Image and Video Retrieval (CIVR 07) July 2007 Amsterdam, The Netherlands; 
Csurka, G., Dance, C., Fan, L., Willamowski, J., Bray, C., Visual categorization with bags of keypoints, pp. 59-74. , Proceedings of the European Conference on Computer Vision (ECCV 04) May 2004 Prague, Czech Republic; 
Marszaek, M., Schmid, C., Spatial weighting for bag-of-features, 2, pp. 2118-2125. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Wang, G., Zhang, Y., Fei-Fei, L., Using dependent regions for object categorization in a generative framework, 2, pp. 1597-1604. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Sivic, J., Zisserman, A., Video Google: A text retrieval approach to object matching in videos, 2, pp. 1470-1477. , Proceedings of the 9th IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Willamowski, J., Arregui, D., Csurka, G., Dance, C.R., Fan, L., Categorizing nine visual classes using local appearance descriptors, , Proceedings of the Workshop on Learning for Adaptable Visual Systems (LAVS), in Conjunction with the International Conference on Pattern Recognition (ICPR 04) August 2004 Cambridge, UK; 
Bosch, A., Zisserman, A., Muoz, X., Scene classification via pLSA, 3954, pp. 517-530. , Proceedings of the 9th European Conference on Computer Vision (ECCV 06) May 2006 Graz, Austria Lecture Notes in Computer Science; 
Hofmann, T., Unsupervised learning by probabilistic latent semantic analysis (2001) Machine Learning, 42 (12), pp. 177-196; 
Blei, D.M., Ng, A.Y., Jordan, M.I., Latent Dirichlet allocation (2003) Journal of Machine Learning Research, 3 (45), pp. 993-1022; 
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Carson, C., Thomas, M., Belongie, S., Hellerstein, J.M., Malik, J., Blob-world: A system for region-based image indexing and retrieval, pp. 509-516. , Proceedings of the 3rd International Conference on Visual Information Systems (VISUAL 99) June 1999 Amsterdam, The Netherlands Springer; 
Duygulu, P., Barnard, K., De Freitas, J.F.G., Forsyth, D.A., Object recognition as machine translation: Learning a lexicon for a fixed image vocabulary, 2353, pp. 97-112. , Proceedings of the 7th European Conference on Computer Vision (ECCV 02) May 2002 Copenhagen, Denmark Lecture Notes in Computer Science; 
Vogel, J., Schiele, B., A semantic typicality measure for natural scene categorization, 3175, pp. 195-203. , Proceedings of the 26th DAGM Pattern Recognition Symposium August-September 2004 Tbingen, Germany Lecture Notes in Computer Science; 
Verbeek, J.J., Triggs, B., Region classification with Markov field aspect models, pp. 1-8. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 07) June 2007 Minneapolis, Minn, USA; 
Shi, J., Malik, J., Normalized cuts and image segmentation, pp. 731-737. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 97) June 1997 San Juan, Puerto Rico, USA; 
Kumar, M.P., Torr, P.H.S., Zisserman, A., Obj cut, 1, pp. 18-25. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 05) June 2005 San Diego, Calif, USA; 
Leibe, B., Leonardis, A., Schiele, B., Combined object categorization and segmentation with an implicit shape model, pp. 17-32. , Proceedings of the 8th European Conference on Computer Vision (ECCV 04) May 2004 Prague, Czech Republic; 
Russell, B.C., Freeman, W.T., Efros, A.A., Sivic, J., Zisserman, A., Using multiple segmentations to discover objects and their extent in image collections, 2, pp. 1605-1612. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Monay, F., Quelhas, P., Odobez, J.-M., Gatica-Perez, D., Integrating co-occurrence and spatial contexts on patch-based scene segmentation, p. 14. , Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshop (CVPR 06) June 2006 New York, NY, USA; 
Cao, L., Fei-Fei, L., Spatially coherent latent topic model for concurrent object segmentation and classification, , Proceedings of the 11th IEEE International Conference on Computer Vision (ICCV 07) October 2007 Rio de Janeiro, Brazil; 
Liu, D., Chen, T., Background cutout with automatic object discovery, 4, pp. 345-348. , Proceedings of IEEE International Conference on Image Processing (ICIP 07) September 2007 San Antonio, Tex, USA; 
Tuytelaars, T., Van Gool, L., Content-based image retrieval based on local affinely invariant regions, 1614, pp. 493-500. , Proceedings of the 3rd International Conference on Visual Information and Information Systems June 1999 Amsterdam, The Netherlands Lecture Notes in Computer Science; 
Mikolajczyk, K., Schmid, C., A performance evaluation of local descriptors, 2, pp. 257-263. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 03) June 2003 Madison, Wis, USA; 
Mikolajczyk, K., Tuytelaars, T., Schmid, C., A comparison of affine region detectors (2005) International Journal of Computer Vision, 65 (12), pp. 43-72; 
Baeza-Yates, R., Ribeiro-Neto, B., (1999) Modern Information Retrieval, , New York, NY, USA ACM Press; 
Monay, F., Quelhas, P., Gatica-Perez, D., Odobez, J.-M., Constructing visual models with a latent space approach, 3940, pp. 115-126. , Proceedings of the Subspace, Latent Structure and Feature Selection, Statistical and Optimization, Perspectives Workshop (SLSFS 05) February 2006 Bohinj, Slovenia Lecture Notes in Computer Science; 
Fergus, R., Fei-Fei, L., Perona, P., Zisserman, A., Learning object categories from Googles image search, 2, pp. 1816-1823. , Proceedings of the 10th IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Buntine, W.L., Variational extensions to em and multinomial PCA, 2430, pp. 23-34. , Proceedings of the 13th European Conference on Machine Learning August 2002 Helsinki, Finland Lecture Notes in Computer Science; 
Bishop, C., (1995) Neural Networks for Pattern Recognition, , Oxford, UK Oxford University
},
	month = {2009///},
	abstract = {We present a novel approach for contextual classification of image patches in complex visual scenes, based on the use of histograms of quantized features and probabilistic aspect models. Our approach uses context in two ways: (1) by using the fact that specific learned aspects correlate with the semantic classes, which resolves some cases of visual polysemy often present in patch-based representations, and (2) by formalizing the notion that scene context is image-specificwhat an individual patch represents depends on what the rest of the patches in the same image are. We demonstrate the validity of our approach on a man-made versus natural patch classification problem. Experiments on an image collection of complex scenes show that the proposed approach improves region discrimination, producing satisfactory results and outperforming two noncontextual methods. Furthermore, we also show that co-occurrence and traditional (Markov random field) spatial contextual information can be conveniently integrated for further improved patch classification.},
	isbn = {16875176 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-61449241990\&partnerID=40\&md5=b909ab8585061d7873ec0fe14a47dba8},
	author = {Monay, F. and Quelhas, P. and Odobez, J.-M. and Gatica-Perez, D.}
}
@book {2295,
	title = {Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5627 LNCS},
	year = {2009},
	note = {Conference code: 77746Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02611-9_81Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica Divis{\~a}o de Sinal e Imagem, Campus FEUPReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, p. 13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Willemse, J., Kulikova, O., de Jong, H., Bisseling, T., A new whole-mount dna quantification method and the analysis of nuclear dna content in the stem-cell niche of arabidopsis roots (2008) The Plant Journal, 10, pp. 1365-1374; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Schmitt, M.H.O., Morphological multiscale decomposition of connected regions with emphasis on cell clusters (2009) Computer Vision and Image Understanding, 113, pp. 188-201; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. on Circuits and Systems I-Regul. Papers, 53 (11), pp. 2405-2414; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proceedings of the Int. Conference on Image Analysis and Processing, pp. 757-761; 
},
	month = {2009///},
	pages = {824 - 831},
	address = {Halifax, NS},
	abstract = {The study of cell nuclei is fundamental for plant cell Biology research. To obtain information at cellular level, researchers image cells{\textquoteright} nuclei which were modified with fluorescence proteins, through laser scanning confocal microscopy. These images are normally noisy and suffer from high background fluorescence, making grey-scale segmentation approaches inadequate for a usable detection. To obtain a successful detection even at low contrast we investigate the use of a particular convergence filter, the Symmetric Sliding Band filter (SSBF), for cell detection. This filter is based on gradient convergence and not intensity. As such it can detect low contrast cell nuclei which otherwise would be lost in the background noise. Due to the characteristics of cell nuclei morphology, a symmetry constrain is integrated in the filter which corrects some inadequate detections and results in a filter response that is more discriminative. We evaluate the use of this filter for cell nuclei detection on the Arabidopsis thaliana root tip, where the nuclei were stained using yellow fluorescence protein. The resulting cell nuclei detection precision is 89\%. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {Arabidopsis thaliana, Background noise, Band filters, Bioinformatics, Biology imaging, Cell detection, Cell membranes, cell nucleus, Cellular levels, confocal microscopy, Convergence filters, cytology, Filter response, Fluorescence, Fluorescence proteins, Fluorescent images, Image analysis, Laser scanning confocal microscopy, Low contrast, Plant cell culture, Plant cells, Positron emission tomography, Root tip, Yellow fluorescence proteins},
	isbn = {03029743 (ISSN); 3642026109 (ISBN); 9783642026102 (ISBN)},
	author = {Marcuzzo, M. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@conference {2338,
	title = {Automatic cell segmentation from confocal microscopy images of the Arabidopsis root},
	booktitle = {2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBIIEEE Int. Symp. Biomed. Imaging: Nano Macro, Proc., ISBI},
	series = {2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI},
	year = {2008},
	note = {Conference code: 73366Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusArt. No.: 4541095doi: 10.1109/ISBI.2008.4541095Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A., Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo (2007) Motion07, pp. 13-13; 
Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of arabidopsis thaliana root meristem in confocal microscopy (2004) Proc. of the Inter. Conf. on Image Analysis and Recognition, pp. 166-174; 
Perona, P., Malik, J., Scale-space and edge detection using anisotropic diffusion (1990) IEEE Trans. on PAMI, 7 (12), pp. 629-639; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095. , August; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations IEEE Trans. on PAMI, 13 (6); 
Meyer, F., Beucher, S., Morphological segmentation (1990) JVCIR, 1 (1), pp. 21-46. , Sept; 
Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Underst, 77 (3), pp. 317-370; 
Kovesi, P., Symmetry and asymmetry from local phase (1997) Proc. of the Tenth Australian Joint Conf. on Artificial Intelligence, , December; 
Burges, C., A tutorial on support vector machines for pattern recognition (1998) Data Mining and Knowledge Discovery, 2 (2), pp. 121-167
Sponsors: Institute of Electrical and Electronic Engineers; Engineering in Medicine and Biology Society; Signal Processing Society},
	month = {2008///},
	pages = {712 - 715},
	address = {Paris},
	abstract = {In vivo observation and tracking of cell division in the Arabidopsis thaliana root meristem, by time-lapse confocal microscopy, is central to biology research. The research herein described is based on large amount of image data, which must be analyzed to determine the location and state of cells. The possibility of automating the process of cell detection/marking is an important step to provide research tools to the biologists in order to ease the search for a special event as cell division. This paper discusses an automatic cell segmentation method, which selects the best cell candidates from a starting watershed based image segmentation. The selection of individual cells is obtained using a Support Vector Machine (SVM) classifier, based on the shape and edge strength of the cells{\textquoteright} contour. The resulting segmentation is largely pruned of badly segmented cells, which can reduce the false positive detection of cell division. This is a good result on its own and a starting point for improvement of cell segmentation methodology. {\textcopyright}2008 IEEE.},
	keywords = {Biological cells, Biomedical image processing, Cell divisions, cell proliferation, Cell segmentation, Cells, confocal microscopy, image processing, Image segmentation, Microscopic examination, Support vector machines, Technical presentations},
	isbn = {9781424420032 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-51049123600\&partnerID=40\&md5=9e4652fa61936d105b4059db95e63f78},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2346,
	title = {A hybrid approach for arabidopsis root cell image segmentation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5112 LNCS},
	year = {2008},
	note = {Conference code: 72695Cited By (since 1996): 3Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-540-69812-8_73Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, pp. 13-13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic Tracking of Arabidopsis thaliana Root Meristem in Confocal Microscopy (2004) LNCS, 3212, pp. 166-174. , Campilho, A.C, Kamel, M, eds, ICIAR 2004, Springer, Heidelberg; 
Perona, P., Malik, J., Scale-space and edge detection using anisotropic diffusion (1990) IEEE Trans. on PAMI, 7 (12), pp. 629-639; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. on PAMI, 13 (6), pp. 583-598; 
Meyer, F., Beucher, S., Morphological segmentation (1990) JVCIR, 1 (1), pp. 21-46; 
Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Underst, 77 (3), pp. 317-370; 
Kovesi, P.: Symmetry and asymmetry from local phase. In: Proc. of the Tenth Australian Joint Conf. on Artificial Intelligence (December 1997)Burges, C., A tutorial on support vector machines for pattern recognition (1998) Data Mining and Knowledge Discovery, 2 (2), pp. 121-167
},
	month = {2008///},
	pages = {739 - 749},
	address = {Povoa de Varzim},
	abstract = {In vivo observation and tracking of the Arabidopsis thaliana root meristem, by time-lapse confocal microscopy, is important to understand mechanisms like cell division and elongation. The research herein described is based on a large amount of image data, which must be analyzed to determine the location and state of cells. The automation of the process of cell detection/marking is an important step to provide research tools for the biologists in order to ease the search for special events, such as cell division. This paper discusses a hybrid approach for automatic cell segmentation, which selects the best cell candidates from a starting watershed-based image segmentation and improves the result by merging adjacent regions. The selection of individual cells is obtained using a Support Vector Machine (SVM) classifier, based on the shape and edge strength of the cells{\textquoteright} contour. The merging criterion is based on edge strength along the line that connects adjacent cells{\textquoteright} centroids. The resulting segmentation is largely pruned of badly segmented and over-segmented cells, thus facilitating the study of cell division. {\textcopyright} 2008 Springer-Verlag Berlin Heidelberg.},
	keywords = {Arabidopsis (Arabidopsis thaliana), Arabidopsis thaliana (ELIP), Cell divisions, Cell image segmentation, cell proliferation, Cell segmentation, Cells, confocal microscopy, Digital image storage, Heidelberg (CO), Hybrid approaches, Image analysis, Image data, image processing, Image segmentation, Imaging techniques, In vivo observation, Individual (PSS 544-7), International conferences, Merging, Microscopic examination, Research tools, Root meristem, Strength (IGC: D5/D6), Support Vector Machine (SVM), Support vector machines, Time lapse confocal microscopy},
	isbn = {03029743 (ISSN); 3540698116 (ISBN); 9783540698111 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47749134578\&partnerID=40\&md5=3b05fc0d5d004216e681d9c3e2199aac},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@conference {2333,
	title = {Tracking of Arabidopsis thaliana root cells in time-lapse microscopy},
	booktitle = {Proceedings - International Conference on Pattern RecognitionProc. Int. Conf. Pattern Recognit.},
	series = {2008 19th International Conference on Pattern Recognition, ICPR 2008},
	year = {2008},
	note = {Conference code: 81859Export Date: 21 November 2012Source: ScopusArt. No.: 4761746CODEN: PICRELanguage of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPPortugalReferences: Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526;Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627; 
Dabov, K., Foi, A., Katkovnik, V., Egiazarian, K., Image denoising by sparse 3d transform-domain collaborative filtering (2007) IEEE Trans. on Image Processing, 16 (8), pp. 2080-2095. , August; 
Garcia, B., Campilho, A., Scheres, B., Campilho, A., Automatic tracking of arabidopsis thaliana root meristem in confocal microscopy (2004) Proc. of the Inter. Conf. on Image Analysis and Recognition, pp. 166-174. , Springer LCNS 3212; 
Harris, C., Stephens, M., A combined corner and edge detector (1988) Proc. of the 4th Alvey Vision Conf.; 
Hartley, R., Zisserman, A., (2003) Multiple View Geometry in Computer Vision, , Cambridge University Press; 
Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res., 119, pp. 85-93; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. on PAMI, 13 (6), pp. 583-598; 
Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., A hybrid approach for cell image segmentation (2008) Proc. of the ICIAR, pp. 739-749. , Springer LCNS 5112; 
Meyer, F., Beucher, S., Morphological segmentation (1990) JVCIR, 1 (1), pp. 21-46. , Sept; 
Mohinder, S., Angus, P., (1993) Kalman Filtering Theory and Practice, , Prentice Hall; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A., Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo (2007) Motion07, pp. 13-13
},
	month = {2008///},
	address = {Tampa, FL},
	abstract = {In vivo observation of cells in the Arabidopsis thaliana root, by time-lapse confocal microscopy, is central to biology research. The research herein described is based on large amount of image data, which must be analyzed to determine the location and state of individual cells. Automating the process of cell tracking is an important step to create tools which will facilitate the analysis of cells{\textquoteright} evolution through time. Here we introduce a confocal tracking system designed in two stages. At the image acquisition stage, we track the area under analysis based on point-to-point correspondences and motion estimation. After image acquisition, we compute cell-to-cell correspondences through time. The final result is a temporal structured information about each cell. {\textcopyright} 2008 IEEE.},
	keywords = {Arabidopsis thaliana, Bioinformatics, Cell tracking, confocal microscopy, Image acquisition, Image data, In-vivo, Individual cells, Motion estimation, Pattern recognition, Point correspondence, Root cells, Structured information, Time-lapse microscopy, Tracking system, Two stage},
	isbn = {10514651 (ISSN); 9781424421756 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77957963663\&partnerID=40\&md5=9794c92c760039ecd3cab6b8a89fa8e3},
	author = {Marcuzzo, M. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2413,
	title = {Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {309-311 I},
	year = {2006},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterlais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Duncan, A.C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosensors and Bioelectronics, 17, pp. 413-426;Walboomers, X.F., Contact guidance of rat fibroblasts on various implant materials (1999) Journal of Biomedical Materials Research, 47, pp. 204-212; 
Queiroz, A.C., Laser surface modifications of hydroxyapatite and glass-reinforced hydroxyapatite (2004) Biomaterials, 25, pp. 4607-4614; 
Ahmad, M., McCarthy, M.B., Gronowicz, G., An in vitro model for mineralization of human osteoblasts-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220
},
	month = {2006///},
	pages = {105 - 108},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture and microchemistry. The surface topography ultimately affects the nature and the strength of the interactions that occur at biomaterial-biological environment (cell adhesion, mobility, spreading and proliferation). Thus, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (for instance, cells). [1] There are a number of substrates that already have been studied (such as silicone, polystyrene, poly-L-lactic acid and titanium coated polystyrene) in terms of surface topography. [2] However, few studies are related to hydroxyapatite substrates. As it is well established, hydroxyapatite is a well known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense hydroxyapatite substrates was altered by using KFr excimer laser. Excimer lasers produce high-intensity, pulsed ultraviolet radiation and are especially well suited for materials processing due to their large beam cross-section area, which permits using mask projection technologies to process relatively large areas in a single step.[3].},
	keywords = {Bone, cell adhesion, Cells, Ceramic materials, Excimer laser, Excimer lasers, hydroxyapatite, Microtexture, Microtopography, morphology, Strength of materials, Surface modification, Surface topography, Surface treatment},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645413875\&partnerID=40\&md5=0234521c5cb850a0ff51ca4c64c39074},
	author = {Teixeira, S. and Queiroz, A.C. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@book {2429,
	title = {Adsorption isotherm of sodium ampicillin onto dense and porous hydroxyapatite},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, Rua do Campo Alegre, 823, 4150-160 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Nikolakakis, I., Newton, J.M., Malamataris, S., (2002) European Journal of Pharmaceutical Sciences, 17 (4-5), pp. 229-238;Barroug, A., Glimcher, M.J., (2002) Journal of Orthopaedic Research, 20 (2), pp. 274-280; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterials, 22 (11), pp. 1393-1400; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2004) Key Enginnering Materials, 254-256, pp. 997-1000
},
	month = {2005///},
	pages = {387 - 390},
	abstract = {The adsorption behaviour of sodium ampicillin to dense and porous hydroxyapatite as been extensively studied through the obtention of an adsorption isotherm. This isotherm was produced at body temperature, and a wide range of antibiotic solutions were used. The adsorption behaviour was measured by UV spectroscopy. The shape of the isotherm for the two forms of the material,dense and porous, indicates that the adsorption behaviour of the antibiotic seems to be different, however this is due to the adsorption taking place within the pores of the porous material, thus showing a larger amount of antibiotic adsorbed.},
	keywords = {Adsorption, Adsorption behavior, Adsorption isotherms, ampicillin, Antibiotics, hydroxyapatite, Porous hydroxyapatite, Porous materials, Sodium ampicillin, Sodium compounds, Ultraviolet spectroscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249009270\&partnerID=40\&md5=1a365e0d97db937727266c9387789cdc},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J.}
}
@article {2451,
	title = {Development of a system to adsorb drugs onto calcium phosphate materials},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {16},
	year = {2005},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-005-2535-3PubMed ID: 15965596Language of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: aqueiroz@ineb.up.ptChemicals/CAS: ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; hydroxyapatite, 1306-06-5, 51198-94-8; metronidazole, 39322-38-8, 443-48-1; Ampicillin, 69-53-4; Anti-Infective Agents; calcium phosphate, 10103-46-5; Calcium Phosphates; Coated Materials, Biocompatible; Delayed-Action Preparations; Drug Carriers; Metronidazole, 443-48-1References: Seymour, R.A., Heasman, P.A., Macgregor, I.D.M., (1992) Drugs, Diseases, and the Periodontium, 1. , edited by R. A. Seymour and P. A. Heasman Oxford University Press, Oxford;Carnevale, R.A., Greco, G.W., Bullard, C.M., Hanker, J.S., (1987) Biomedical Materials and Devices, p. 247. , edited by J. S. Hanker and B. L. Giammara Materials Research Society, Pittsburgh; 
Friedman, M., Steinberg, D., (1990) Pharm. Res., 7, p. 313; 
Hagiwara, S., Takamatsu, N., Tominaga, Y., Umeda, M., (1998) J. Period., 69, p. 62; 
Wright, T.L., Ellen, R.P., Lacroix, J.M., Sinnadurai, S., Mittelman, M.W., (1997) J. Period. Res., 32, p. 473; 
Golomb, G., Friedman, M., Soskolne, A., Stabholz, A., Sela, M.N., (1984) J. Dent. Res., 63, p. 1149; 
Goodson, J.M., Hogan, P.E., Dunham, S.L., (1984) J. Period., 55, p. 81; 
Somayaji, B.V., Jariwala, U., Jayachandran, P., Vidyalakshim, K., Dudhani, R.V., (1998) J. Period., 69, p. 409; 
Stoltze, K., (1992) J. Clin. Period., 19, p. 698; 
Goodson, J.M., Holborow, D., Dunn, R.L., Hogan, P., Dunham, S., (1983) J. Period., 54, p. 575; 
Yu, D., Wong, J., Matsuda, Y., Fox, J.L., Higuchi, W.I., Otsuka, M., (1992) J. Pharm. Sci., 81, p. 529; 
Nishihara, K., (1993) Clin. Mater., 12, p. 159; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mat. Res., 45, p. 376; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mat. Res., 47, p. 603; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mat. Res., 54, p. 463; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterials, 22, p. 1393; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Knowles, J.C., Santos, J.D., Hastings, G.W., World Patent, WO0068164 (1994)Myers, H.M., Tochon-Danguy, H.J., Baud, C.A., (1983) Calcif. Tissue Int., 35, p. 745; 
Ratier, A., Gibson, I.R., Best, S.M., Freche, M., Lacout, J.L., Rodrigues, F., (2001) Biomaterials, 22, p. 897; 
Ando, D.J., (1996) Ultraviolet and Visible Spectroscopy, p. 16. , John Wiley \& Sons, West Sussex
},
	month = {2005///},
	pages = {641 - 646},
	abstract = {Several studies were carried out in order to reduce the systemic use of antibiotics due to the high concentration required to provide the minimum inhibitory concentration (MIC) at infected sites. The aim of this study was to develop a system of drug adsorption onto commercial hydroxyapatite (HA, Ca                        10(PO
                        4)
                        6(OH)
                        2) and glass reinforced hydroxyapatite (GR-HA) granules. The drug will then be released for the local treatment of periodontitis. The antibiotics used in this study were metronidazole, a specific antibiotic indicated for the systemic treatment of periodontitis, and ampicillin, a wide spectrum antibiotic. UV spectroscopy was used to measure the amount of drug adsorbed onto HA and GR-HA granules. Results showed that metronidazole was unable to adsorb on the material{\textquoteright}s surface, as opposed to ampicillin which adsorbed both onto HA and GR-HA. Preliminary release kinetics studies were carried out using a flow through dissolution system. Results are discussed in terms of the influence of the different surface characteristics of the materials on the adsorption processes. {\textcopyright} 2005 Springer Science + Business Media, Inc.
},
	keywords = {Adsorption, ampicillin, Anti-Infective Agents, Antibiotics, article, Calcium Compounds, calcium phosphate, Calcium phosphates, Coated Materials, Biocompatible, concentration response, Delayed-Action Preparations, Diffusion, Dissolution, drug absorption, drug adsorption, Drug Carriers, Drug release, drug stability, glass, Glass reinforced hydroxyapatie (GR-HA), human, Humans, hydroxyapatite, Kinetics, Material{\textquoteright}s surface, Materials science, materials testing, metronidazole, minimum inhibitory concentration, Minimum inhibitory concentration (MIC), periodontitis, priority journal, Reaction kinetics, reduction, surface property, Ultraviolet spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-21344438213\&partnerID=40\&md5=5963a17bf67c04ab22d05ce113fa12b0},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J.}
}
@book {2424,
	title = {Porous HA scaffolds for drug releasing},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {284-286},
	year = {2005},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB, Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Queiroz, A.C., (2001) Biomaterials, 22, p. 1393},
	month = {2005///},
	pages = {407 - 410},
	abstract = {Dense and porous hydroxyapatite materials aimed at being used in controlled drug delivery, were characterised and studied in order to evaluate their ability to adsorb and release sodium ampicillin in a controlled manner, as a model for a drug delivery system, potentially applicable associated to surgery for the treatment of bone defects. These porous materials should also have adequate mechanical strength to withstand manipulation and sculpturing in surgery room. Adsorption and release profiles were obtained for a range of porous materials, leading to higher adsorption rates and more adequate release profiles than for dense materials.},
	keywords = {Adsorption, ampicillin, Controlled drug delivery, Drug release, hydroxyapatite, orthopedics, Porous hydroxyapatite, Porous hydroxyapatites, Porous materials, Scaffolds, Sodium ampicillin, Surgery, Surgery rooms},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34248995543\&partnerID=40\&md5=db9f063f6d3b98c88a9d87c693ada572},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J.}
}
@article {2475,
	title = {Laser surface modification of hydroxyapatite and glass-reinforced hydroxyapatite},
	journal = {BiomaterialsBiomaterials},
	volume = {25},
	year = {2004},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2003.11.054PubMed ID: 15120506Language of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB-Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: aqueiroz@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Durapatite, 1306-06-5; Macromolecular SubstancesReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical evaluation of porous and nonporous hydroxyapatite in the treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, pp. 1-10. , I.D.M. MacGregor. Oxford: Oxford University Press; 
Systemic antibiotics in periodontics (1996) J Periodontol, 67, pp. 831-838; 
Steinberg, D., Friedman, M., Soskolne, A., Sela, M.N., A new degradable controlled release device for treatment of periodontal disease: In vitro release study (1990) J Periodontol, 61, pp. 393-398; 
Soskolne, W.A., Heasman, H.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis: A multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Prado Da Silva, M.H., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., Porous glass reinforced hydroxyapatite materials produced with different organic additives (2002) J Non-Cryst Solids, 304, pp. 286-292; 
Barinov, S.M., Baschenko, Y.V., Application of ceramic composits as implants: Result and problem (1992) Bioceramics and the Human Body, pp. 206-210. , A. Krajewski. UK: Elsevier Science Publishers; 
Koshino, T., Murase, T., Takagi, T., Saito, T., New bone formation around porous hydroxyapatite wedge implanted in opening wedge high tibial osteotomy in patients with osteoarthritis (2001) Biomaterials, 22, pp. 1579-1582; 
Nishihara, K., Studies on peri-root tissue formation around new type artificial root made of dense hydroxyapatite (1993) Clin Mater, 12, pp. 159-167; 
Chang, B., Lee, C., Hong, K., Youn, H., Ryu, H., Chung, S., Park, K., Osteoconduction at porous hydroxyapatite with various pore configurations (2000) Biomaterials, 21, pp. 1291-1298; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., Reinforcement of hydroxyapatite by adding P 2O 5-CaO glasses with Na 2O, K 2O and MgO (1996) J Mater Sci: Mater Med, 7, pp. 187-189; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings (1999) J Biomed Mater Res, 47, pp. 603-611; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P2O5 Glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J Biomed Mater Res, 54, pp. 463-469; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., Adsorption and release of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites (2001) Biomaterials, 22, pp. 1393-1400; 
Cl{\`e}ries, L., Fern{\'a}ndez-Pradas, J.M., Morenza, J.L., Behaviour in simulated body fluid of calcium phosphate coating obtained by laser ablation (2000) Biomaterials, 21, pp. 1861-1865; 
D{\textquoteright}Alession, L., Teghil, R., Zaccardo, I., Ferro, D., Marotta, V., Pulsed laser ablation and deposition of bioactive glass as coating material for biomedical applications (1999) Appl Surf Sci, 138-139, pp. 527-532; 
Fern{\'a}ndez-Pradas, J.M., Cl{\`e}ries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the depositon rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys a, 72, pp. 613-618; 
Zeng, H., Lacefield, W.R., XPS, EDX and FTIR analysis of pulsed laser deposited calcium phosphate bioceramic coatings: The effects of various process parameters (2000) Biomaterials, 21, pp. 23-30; 
Serra, P., Fern{\'a}ndez-Pradas, J.M., Sardin, G., Morenza, J.L., Interaction effects of an excimer laser beam with hydroxyapatite targets (1997) Appl Surf Sci, 109-110, pp. 384-388; 
Serra, P., Morenza, J.L., Fluence dependence of hydroxyapatite laser ablantion plumes (1998) Thin Solid Films, 335, pp. 43-48; 
Foltyn, S.R., Dye, R.C., Ott, K.C., Peterson, E., Hubbard, K.M., Hutchinson, W., Muenchausen, R.E., Wu, X.D., Target modification in the excimer laser deposition of YBa 2Cu 3O 7-x thin films (1991) Appl Phys Lett, 59, pp. 594-596; 
Oliveira, V., Conde, O., Vilar, R., UV laser micromachining of ceramic materials: Formation of columnar topographies (2001) Adv Eng Mater, 3, pp. 75-81; 
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Khor, K.A., Vreeling, A., Dong, Z.L., Cheang, P., Laser treatment of plasma sprayed HA coatings (1999) Mater Sci Eng a, 266, pp. 1-7; 
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Rey, C., Shimizu, M., Collins, B., Glimcher, M.J., Resolution enhanced Fourier transform infrared spectroscopy study of the environment of phosphate ion in the early deposits of a solid phase calcium phosphate in bone and enamel and their evolution with age: Investigations in the ν 3 PO 4 3- domain (1991) Calcif Tissue Int, 49, pp. 383-388; 
Elliott, J.C., Structure and chemistry of apatites and other calcium orthophosphates (1994) Inorganic Chemistry, 18, p. 50. , Elliott J.C. Amsterdam: Elsevier Science; 
Dupraz, A., Nguyen, T.P., Richard, M., Dalcusi, G., Passuti, N., Influence of a cellulosic ether carrier on the structure of biphasic calcium phosphate ceramic particles in an injectable composite material (1999) Biomaterials, 20, pp. 663-673; 
Franke, R., Chass{\'e}, Th., Streubel, P., Meisel, A., Auger parameters and relaxation energies of phosphorus in solid compounds (1991) J Electron Spectrosc Relat Phemon, 56, pp. 381-388; 
Heitz, J., Pedarning, J.D., Bauerle, D., Petzow, G., Excimer laser ablation and micro-patterning of ceramic Si 3N 4 (1997) Appl Phys a, 65, pp. 259-261; 
Foltyn, S.R., Surface modification of materials by cumulative laser irradiation (1994) Pulsed Laser Deposition of Thin Films, pp. 89-113. , D.B. Chrisey, \& G.K. Hubler. New York: Wiley; 
Lappalainen, J., Frantti, J., Lanto, V., Surface structure and particulate generation in Pb 0.97Nd 0.02(Zr 0.55Ti 0.45)O 3 ablation targets during pulsed laser deposition (1999) J Am Cer Soc, 82, pp. 889-896; 
{\'O}brien, T.P., Lawler, J.F., Lunney, J.G., Blau, W.J., The effect of laser fluence on the ablation and deposition of YBa 2Cu 3O 7 (1992) Mater Sci Eng, 13, pp. 9-13; 
Man, H.C., Zhang, X.M., Yue, T.M., Lau, W.S., Excimer-laser surface modification of engineering ceramics for adhesive bonding (1997) J Mater Proc Techn, 66, pp. 123-129; 
S{\'a}nchez, F., Morenza, J.L., Trtik, V., Characterization of the progressive growth of columns by excimer laser irradiation of silicon (1999) Appl Phys Lett, 75, pp. 3303-3305; 
Pedraza, A., Fowlkes, J.D., Lowndes, D.H., Self organized silicon microcolumns arrays generated by pulsed laser irradiation (1999) Appl Phys a, 69, pp. 731-S734; 
Usoskin, A., Freyhardt, H.C., Krebs, H.U., Influence of light scattering on the development of laser induced ridge-cone structure on target surfaces (1999) Appl Phys a, 69, pp. 823-S826
},
	month = {2004///},
	pages = {4607 - 4614},
	abstract = {Surface treatment of materials with excimer laser radiation often results in the formation of a rough columnar or cone-shaped surface topography, which leads to a considerable increase in the surface area. As a result, the search for a non-porous bioactive material with adequate mechanical properties and a high surface to volume ratio, similar to porous materials, which could be used for drug delivery in the treatment of periodontitis, justified assessing excimer laser surface treatment to promote controlled roughning of hydroxyapatite (HA) and glass-reinforced hydroxyapatite (GR-HA). A KrF excimer laser with 248nm radiation wavelength and 30ns pulse duration was used for surface modification. The laser treatment was carried out in air, using wide ranges of radiation fluence and number of laser pulses. In order to identify the physico-chemical changes induced by the laser treatment and the column formation mechanisms in these materials, the treated surfaces were characterised by laser profilometry, scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infra-red spectroscopy (FTIR). Laser processing induced the formation of a surface topography consisting of cone-shaped features. The constitution of the surface layer was also modified, as revealed by FTIR, XPS and XRD. This work has shown that laser surface modification increases the surface area of HA and GR-HA and is a promising technique to increase the reactivity and drug delivery capability of both materials. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {article, Biocompatible Materials, Biomaterials, drug delivery system, Durapatite, Excimer laser, Excimer lasers, Fourier transform infrared spectroscopy, FTIR, glass, hydroxyapatite, infrared spectroscopy, laser, Lasers, Macromolecular Substances, materials testing, Molecular Conformation, Non-porous bioactive materials, periodontitis, physical chemistry, porosity, priority journal, Profilometry, Radiation Dosage, Reinforcement, Scanning electron microscopy, Surface area, Surface modification, Surface Properties, Surface treatment, topography, X ray diffraction, X ray diffraction analysis, X ray photoelectron spectroscopy, XPS, XRD},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2342427815\&partnerID=40\&md5=24da3c08c0c7b636d9d5039812944b52},
	author = {Queiroz, A.C. and Santos, J.D. and Vilar, R. and Eug{\'e}nio, S. and Monteiro, F.J.}
}
@book {2497,
	title = {Porous Hydroxyapatite and Glass Reinforced Hydroxyapatite for Controlled Release of Sodium Ampicillin},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., (1987) J Periodontol, 58, pp. 521-528;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, pp. 1-10. , MacGregor IDM, editor. Oxford: Oxford University Press; 
Steinberg, D., Friedman, M., Soskolne, A., Sela, M.N., (1990) J Periodontol, 61, pp. 393-398; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., (1996) J Mater Sci: Mater Med, 7, pp. 187-189; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J Biomed Mat Res, 45, pp. 376-383; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Key Engineering Materials, , submitted; 
Nishihara, K., (1993) Clin Materials, 12, pp. 159-167; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterials, 22, pp. 1393-1400
},
	month = {2004///},
	pages = {997 - 1000},
	address = {Porto},
	abstract = {Porous hydroxyapatite (HA) and glass reinforced hydroxyapatite (GRHA) with adequate macro and microporous structure were developed, aiming at being used as drug delivery carrier of antibiotics, for the in situ treatment of periodontitis. Materials were characterised by XRD and FTIR, presenting no changes from similar dense materials. Mercury intrusion porosimetry revealed micropores of less than 1 μm, accounting for 15\% of the total porosity. Compression tests have shown close values for HA and GRHA, with the former showing slightly higher values of strength. Ampicillin adsorption was more effective on porous than on dense HA, and was similar for HA and GRHA.},
	keywords = {Adsorption, Antibiotics, Coatings, Dense materials, Diseases, Drug delivery, Drug dosage, Fourier transform infrared spectroscopy, glass, Glass reinforced hydroxyapatite, hydroxyapatite, Mercury (metal), Porous, Porous materials, Scanning electron microscopy, Sodium, Strength of materials, Ultraviolet spectroscopy, X ray diffraction analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516111\&partnerID=40\&md5=a85b6393d2a4a2d3ee4b384bf4c3de78},
	author = {Queiroz, A.C. and Teixeira, S. and Santos, J.D. and Monteiro, F.J.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2477,
	title = {Production of porous hydroxyapatite with potential for controlled drug delivery},
	series = {Materials Science ForumMater Sci Forum},
	volume = {455-456},
	year = {2004},
	note = {Conference code: 63332Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: MSFOELanguage of Original Document: EnglishCorrespondence Address: Queiroz, A.C.; INEB, Inst. de Engenharia Biom{\'e}dica, R do Campo Alegre, 823, 4150-180 Porto, Portugal; email: aqueiroz@ineb.up.ptReferences: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., (1987) J. Periodontology, 58, p. 521;Seymour, R.A., Heasman, P.A., MacGregor, I.D.M., The pathogenesis of periodontal disease (1992) Drugs, Diseases, and the Periodontium, , Oxford University Press; 
(1996) Journal of Periodontology, 67, p. 831; 
Soskolne, W.A., Heasman, H.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., (1997) Journal of Periodontology, 68, p. 32; 
Da Silva, M.H.P., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., (2002) J. of Non-crystaline Solids, 304, p. 286; 
Chang, B., Lee, C., Hong, K., Youn, H., Ryu, H., Chung, S., Park, K., (2000) Biomat., 21, p. 1291; 
Ewers, R., Simons, B., (1992) Biomaterials - Hard Tissue Repair and Replacement, , Elsevier Sc; 
Ohgushi, H., Okumura, M., Yoshikawa, T., Inoue, K., Senpuku, N., Tamai, S., Shors, E.C., (1992) Journal of Biomedical Materials Research, 26, p. 885; 
Barinov, S.M., Baschenko, Y.V., (1992) Bioceramics and the Human Body, , Elsevier Sc; 
Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., (1997) J. Mater. Sc.: Materials in Medicine, 8, p. 731; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2003) Bioceramics, , accepted for; 
Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., (2001) Biomaterial, 22, p. 1393
},
	month = {2004///},
	pages = {358 - 360},
	address = {Caparica},
	abstract = {Porous hydroxyapatite was prepared in order to be used as a drug carrier and promote bone ingrowth. For this purpose, the microstructure should have interconnective pores and micro as well as macro porosity, for bone cell adhesion and bone ingrowth. The porous hydroxyapatite prepared by the burn-out method exhibits all these features. For measuring porosity, SEM analysis, mercury intrusion porosimetry and BET were used. FT-IR and XRD analysis revealed that only hydroxyapatite phase was present, being this result similar to the one found for dense samples. The morphology obtained appears to be well adapted to bone growth and drug adsorption.},
	keywords = {Adsorption, Antibiotics, Bioceramics, Controlled drug delivery, Drug Carriers, Drug delivery, Fourier transform infrared spectroscopy, hydroxyapatite, morphology, Polyurethanes, porosity, Porous, Porous materials, Scanning electron microscopy, X ray diffraction analysis},
	isbn = {02555476 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17044442244\&partnerID=40\&md5=7baa6f22534360d57deb7862546fb275},
	author = {Queiroz, A.C. and Teixeira, S. and Santos, J.D. and Monteiro, F.J.},
	editor = {Martins R. and Fortunato E. and Ferreira I. and Dias C.}
}
@article {2519,
	title = {Dissolution studies of hydroxyapatite and glass-reinforced hydroxyapatite ceramics},
	journal = {Materials CharacterizationMater Charact},
	volume = {50},
	year = {2003},
	note = {Conference code: 61615Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: MACHEdoi: 10.1016/S1044-5803(03)00092-5Language of Original Document: EnglishCorrespondence Address: Prado da Silva, M.H.; DEMP, UFC, Campus PICI, Bl. 714, Fortaleza, CE 60455-760, Brazil; email: mhprado@dem.ufc.brReferences: Barinov, S.M., Baschenko, Y.V., Application of ceramic composites as implants: Result and problem (1992) Bioceramics and the Human Body, pp. 206-210. , A. Ravaglioli, \& A. Krajewski. United Kingdom: Elsevier;Nishihara, K., Studies on peri-root tissue formation around new type artificial root made of dense hydroxyapatite (1993) Clin. Mater., 12, pp. 159-167; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J. Biomed. Mater. Res., 45, pp. 370-375; 
Santos, J.D., Silva, P.L., Knowles, J.C., Talal, S., Monteiro, F.J., Reinforcement of hydroxyapatite by adding P 2O 5-CaO glasses with Na 2O, K 2O, MgO (1996) J. Mater. Sci. Mater. Med., 7, pp. 187-189; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings (1999) J. Biomed. Mater. Res., 47, pp. 603-611; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P 2O 5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J. Biomed. Mater. Res., 45, pp. 376-383; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J. Biomed. Mater. Res., 54, pp. 463-469; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J. Biomed. Mater. Res., 41, pp. 649-656; 
Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., Ca, P-rich layer formed on high-strength bioactive glass-ceramic A-W (1990) J. Biomed. Mater. Res., 24, pp. 331-343; 
Kokubo, T., Kushitani, H., Kitsugi, T., Yamamuro, T., Solution able to reproduce in vivo surface-structure changes of bioactive glass-ceramic A-W (1990) J. Biomed. Mater. Res., 24, pp. 721-734; 
Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., Features of calcium phosphate plasma-sprayed coatings: An in vitro study (1994) J. Biomed. Mater. Res., 28, pp. 961-967; 
Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., Calcium phosphate plasma-sprayed coatings and their stability: An in vivo study (1994) J. Biomed. Mater. Res., 28, pp. 909-917; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Yamamuro, T., Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid (1992) J. Mater. Sci. Mater. Med., 3, pp. 79-83; 
Zhang, H., Li, S., Yan, Y., Dissolution behaviour of hydroxyapatite powder in hydrothermal solution (2001) Ceram. Int., 27, pp. 451-454; 
LeGeros, R.Z., LeGeros, J.P., Calcium phosphate biomaterials in medical applications (1996) Bioceramics, 9, pp. 7-10. , T. Kokubo, T. Nakamura, \& F. Miyaji. Otsu, Japan: Elsevier; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Gibson, I.R., Knowles, J.C., Adsorption and release studies of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites (2001) Biomaterials, 22, pp. 1393-1400; 
Langenbucher, F., Benz, D., K{\"u}rth, W., M{\"o}ller, H., Otz, M., Standardized flow-cell method as an alternative to existing pharmacopoeial dissolution testing (1989) Pharm. Ind., 51 (11), pp. 1276-1281; 
Kokubo, T., (1990) CRC Handbook of Bioactive Ceramics, 1, p. 41. , T. Yamamuro, L.L. Hench, \& J. Wilson. Boca Raton (FL): CRC Press; 
International Standard ISO/FDIS 10993-14, Biological evaluation of medical devices: Part 14 (2001) Identification and Quantification of Degradation Products from Ceramics
},
	month = {2003///},
	pages = {197 - 202},
	address = {Rio de Janeiro},
	abstract = {In the continuous agitation assays, glass-reinforced hydroxyapatite (GR-HA) was shown to form a calcium phosphate (CaP) layer, but hydroxyapatite (HA) only formed dispersed precipitates. The formation of this layer was first detected on the GR-HA with a 7.5\% glass addition (7.5 GR-HA) after only 3 days of immersion in simulated body fluid (SBF). The time required for layer formation decreased as the amount of glass added to the HA increased. The dissolution rate of the materials followed a similar pattern, i.e. the dissolution rate for GR-HA was higher than for HA, and increased with the addition of glass. The immersion of 7.5 GR-HA in water showed almost linear dissolution kinetics over the immersion periods (3, 7, 15, 30 and 60 days). The concentration of calcium ions in solution and the scanning electron microscopy (SEM) analysis of the 7.5 GR-HA specimens immersed in water and in SBF revealed a clear competition between the material dissolution and the precipitation of a CaP phase. Fourier transformed infrared spectroscopy with alternated total reflectance (FTIR-ATR) analysis indicated that the CaP phase that formed during longer immersion times (30 and 60 days) could be a carbonate-substituted CaP precipitate. As expected from previous work, the GR-HA behavior in terms of its in vitro bioactivity is higher than HA because a homogeneous CaP layer is formed and the precipitation occurs faster. From the dissolution test and in accordance with the chemical composition of the samples, GR-HA was more soluble than HA. {\textcopyright} 2003 Elsevier Inc. All rights reserved.},
	keywords = {Ceramic materials, Dissolution, glass, Glass-reinforced hydroxyapatite, hydroxyapatite, Immersion times, Scanning electron microscopy},
	isbn = {10445803 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0141895021\&partnerID=40\&md5=9226464f75e2ecd20d23f9d42e61d69e},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J. and Prado da Silva, M.H.},
	editor = {Solorzano G. and Freire Jr. F.L. and Soares G.A. and Souza P.L.}
}
@article {2544,
	title = {Adsorption and release studies of sodium ampicillin from hydroxyapatite and glass-reinforced hydroxyapatite composites},
	journal = {BiomaterialsBiomaterials},
	volume = {22},
	year = {2001},
	note = {Cited By (since 1996): 55Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(00)00296-9PubMed ID: 11336313Language of Original Document: EnglishCorrespondence Address: Knowles, J.C.; Department of Biomaterials, Eastman Dental Institute, University College London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United Kingdom; email: j.knowles@eastman.ucl.ac.ukChemicals/CAS: Ampicillin, 69-53-4; Durapatite, 1306-06-5; PenicillinsManufacturers: Plasma Biotal, United KingdomReferences: Farag, S.A., Simultaneous liquid chromatographic analysis of the β-lactam antibiotics cefazolin, cefadroxil, cepalexin, ampicillin, and cephradine in solution (1998) J AOAC Int, 81, pp. 381-385;Somayaji, B.V., Jariwala, U., Jayachandran, P., Vidyalakshmi, K., Dudhani, R.V., Evaluation of antimicrobial efficacy and release pattern of tetracycline and metronidazole using a local delivery system (1998) J Periodontol, 69, pp. 409-413; 
Soskolne, W.A., Heasman, P.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis; a multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Steinberg, D., Friedman, M., Soskolne, A., Sela, M.N., A new degradable controlled release device for treatment of periodontal disease; in vitro release study (1990) J Periodontol, 61, pp. 393-398; 
Stoltze, K., Concentration of metronidazole in periodontal pockets after application of a metronidazole 25\% dental gel (1992) J Clin Periodontol, 19, pp. 698-701; 
Ramaglia, L., Sbordone, L., Lenci, F., Gulletta, E., Amato, G., Kennedy, E.B., Microbiology of porous hydroxylapatite implants in human periodontal osseous defects (1986) Biomaterials and clinical applications, 7 vol., pp. 675-680. , Pizzoferrate A., Marachetti P.G., Ravaglioli A., Lee A.J.C. (Eds.), Netherlands: Elsevier; 
Wilson, J., Low, S., Fetner, A., Hench, L.L., Bioactive materials for periodontal treatment; a comparative study (1986) Biomaterials and clinical applications, 7 vol., pp. 223-228. , Pizzoferrate A., Marachetti P.G., Ravaglioli A., Lee A.J.C. (Eds.), Netherlands: Elsevier; 
Kenney, E.B., Lekovic, V., Sa Ferreira, J.C., Han, T., Dimitrijavic, B., Carranza F.A. Jr, Bone formation within porous hydroxyapatite implants in human periodontal defects (1986) J Periodontol, 57, pp. 76-83; 
Krejei, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical evaluation of porous and nonporous hydroxyapatite in treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tensions, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
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Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Osteocompatibility and in vivo evaluation of glass reinforced hydroxyapatite composites (1999) Bioceramics, 12 vol., pp. 421-424. , Ohgushi H., Hastings G.W., Yoshikawa T. (Eds.), Japan: World Scientific; 
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Stoltze K. Concentration of metronidazole in periodontal pockets after application of a metronidazole 25\% dental gel. J Clin Periondontol 1992, 689-701Jones, A.A., Koenman, K.S., Newbold, D.A., Manwell, M.A., Clinical and microbiological effects of controlled release locally delivered minocycline in periodontitis (1994) J Periodontol, 65, pp. 1058-1066
},
	month = {2001///},
	pages = {1393 - 1400},
	abstract = {As a potential therapy for periodontitis, sodium ampicillin, a broad spectrum antibiotic, was adsorbed onto hydroxyapatite (HA) and glass-reinforced hydroxyaptite (GR-HA) composites, and was subsequently released in vitro. The sodium ampicillin, was adsorbed more on HA compared to the GR-HA composites. X-ray diffraction (XRD) and Rietveld analysis were used to identify and quantify the levels of HA and β-tricalcium phosphate (β-TCP) in the microstructure of the GR-HA composites. Lattice parameters changes were observed for the β-TCP phase dependant on the amount of glass added. The release kinetics were shown to be divided into three stages, the first of which where a large amount of sodium ampicillin is released, followed by a slower release rate and then a final stage where the release amount approaches zero, until no more sodium ampicillin was present. X-ray photoeletron spectroscopy (XPS) studies were carried out in order to ensure that the entire antibiotic adsorbed onto the materials had been released. These kinetics studies have indicated the possibility of using these materials as possible carriers for drug delivery. Copyright {\textcopyright} 2001 Elsevier Science Ltd.},
	keywords = {Adsorption, ampicillin, Antibiotics, article, calcium phosphate, composite material, controlled study, drug adsorption, Drug delivery, drug delivery system, Drug release, Durapatite, glass, Glass-reinforced hydroxyapatite, hydroxyapatite, Kinetics, Microstructure, Penicillins, periodontitis, priority journal, Reinforcement, Rietveld analysis, roentgen spectroscopy, Sodium ampicillin, Sodium compounds, structure analysis, unclassified drug, X ray diffraction, X ray diffraction analysis, X ray photoelectron spectroscopy, X-Ray Diffraction},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035372020\&partnerID=40\&md5=72e1b530ae04faed9cceb376c0246aa8},
	author = {Queiroz, A.C. and Santos, J.D. and Monteiro, F.J. and Gibson, I.R. and Knowles, J.C.}
}
@article {2559,
	title = {Self-assembly and surface structure of an amphiphilic graft copolymer, polystyrene-graft-ω-stearyl-poly(ethylene oxide)},
	journal = {Journal of Colloid and Interface ScienceJ. Colloid Interface Sci.},
	volume = {224},
	year = {2000},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: JCISAdoi: 10.1006/jcis.2000.6729Language of Original Document: EnglishCorrespondence Address: Ji, J.; Instituto de Engenharia Biomedica, Laboratorio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jian@ibmc.up.ptChemicals/CAS: macrogol, 25322-68-3; polystyrene, 9003-53-6; stearic acid, 57-11-4, 646-29-7References: Brash, J.L., (1983) Biocompatible Polymer, Metals and Composites, p. 35. , M. Szycher. Lancaster: Technomic;Gombotz, W.R., Guanghui, W., Horbett, T.A., Hoffman, A.S., (1991) J. Biomed. Mater. Res., 25, p. 1547; 
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Gombotz, W.R., Guanhui, W., Horebett, T.A., Hoffman, A.S., (1992) Poly(ethylene Glycol) Chemistry: Biotechnical and Biomedical Application, pp. 247-261. , J. M. Harris. New York: Plenum Press; 
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Hester, J.F., Banerjee, P., Mayes, A.M., (1999) Macromolecules, 32, p. 1643
},
	month = {2000///},
	pages = {255 - 260},
	abstract = {Surface properties of the polystyrene-graft-ω-stearyl-poly(ethylene oxide) (PS-g-SPEO) have been characterized by X-ray photoelectron spectroscopy (XPS), differential scanning calorimetry (DSC), contact angle, and spin probe techniques. The XPS results indicate that the surface and bulk composition of the PS-g-SPEO copolymers differ remarkably from each other. The stearyl and EO components enrich at the copolymer/air interfaces due to the self-assembly of the stearyl groups. At the PS-g-SPEO-72.6 surface (the x in PS-g-SPEO-x indicates the bulk density of the SPEO in wt\%), the self- assembly of the hydrophobic stearyl groups is strong enough to form a stable liquid crystalline phase as indicated by DSC. At polymer/water interfaces, PS-g-SPEO-72.6 presents a hydrophilic surface with low PEO mobility, whereas PS-g-SPEO-50.6 and PS-g-SPEO-31.0 present the hydrophobic surface with high PEO mobility. The two different types of the surfaces, with different characters in surface energy, surface mobility of PEO, and surface architecture of SPEO, will be quite valuable as models for detecting the synergistic action of the PEO chains and the stearyl groups (specific ligand for albumin binding) in protein solutions. (C) 2000 Academic Press.},
	keywords = {Amphiphilic copolymer, article, biomaterial, copolymer, crystal, differential scanning calorimetry, electron microscopy, electron spin resonance, hydrophobicity, macrogol, polystyrene, priority journal, protein assembly, protein binding, protein structure, Self-assembly, stearic acid, Surface structure, X ray analysis},
	isbn = {00219797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034655268\&partnerID=40\&md5=1628a0cfec8d5ced3e11c87d74ceae44},
	author = {Ji, J. and Feng, L. and Qiu, Y. and Yu, X. and Barbosa, M.A.}
}
@article {2581,
	title = {ESR spectroscopy to determine the molecular mobility of poly(ethylene oxide) grafts in amphiphilic graft copolymers},
	journal = {Macromolecular Rapid CommunicationsMacromol. Rapid Commun.},
	volume = {19},
	year = {1998},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: MRCOELanguage of Original Document: EnglishCorrespondence Address: Ji, J.; INEB-mat, Rua do Campo Alegre, 823, 4150 Porto, PortugalReferences: Bots, J.G.F., Van Der Does, L., Bentjes, A., (1986) Biomaterials, 7, p. 393;Osterberg, E., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., Van Alstine, J.M., Harris, J.M., (1995) J. Biomed. Mater. Res., 29, p. 741; 
Hubbell, J.A., (1997) Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), 38, p. 528; 
Harris, J.M., (1997) Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), 38, p. 520; 
Grainger, D.W., Okano, T., Kim, S.W., (1989) J. Colloid Interface Sci., 132, p. 161; 
Qiu, Y.X., Yu, X.J., Feng, L.X., Yang, S.L., (1992) Makromol. Chem., 193, p. 1377; 
Qiu, Y.X., Yu, X.J., Feng, L.X., Yang, S.L., (1992) Chin. J. Polym. Sci., 13, p. 112; 
Ranter, B.D., Graft Copolymer and Block Copolymer Surface (1985) Surface and Interfacial Aspects of Biomedical Polymers, 1, p. 374. , J. D. Andrade, Ed., Plenum Press, New York; 
Andrade, J.D., Gregonis, D.E., Smith, L.M., Polymer surface dynamic (1985) Surface and Interfacial Aspects of Biomedical Polymers, 1, p. 15. , J. D. Andrade, Ed., Plenum Press, New York; 
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Jeon, S.I., Lee, J.H., Andrade, J.D., De Gennes, P.G., (1991) J. Colloid Interface Sci., 142, p. 149; 
Takahara, A., Jo, N.J., Kajiyama, T., (1989) J. Biomater. Sci., Polym. Ed., 1, p. 17; 
Cameron, G.G., ESR Spectroscopy (1989) Comprehensive Polymer Science, Polymer Characterization, p. 517. , S. G. Allen, Ed; 
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Caldararu, H., Caragheorgheopol, A., Donescu, D., Joela, H., (1994) J. Colloid Interface Sci., 173, p. 486
},
	month = {1998///},
	pages = {473 - 477},
	abstract = {The ethylene oxide (EO) mobility in polystyrene-graft-[poly(ethylene oxide)] (PS-g-PEO) and polystyrene-graft-[stearyl poly(ethylene oxide)] (PS-g-SPEO) copolymers was evaluated by spin probe techniques. The ESR spectra indicate that 4-hydroxyl-TEMPO (TEMPO = 2,2,6,6-tetramethylpiperidine-N-oxyl) is strongly biased to the PEO phase of the PS-g-(S)PEO membranes. The rotational correlation time τ                        c can also be employed to assess the PEO mobility in PS-g-(S)PEO membranes. Although τ
                        c of PS-g-(S)PEO usually decreases with increasing surface density of EO, it is of interest that τ
                        c is rather high when the surface within a depth of at least 5 nm is fully occupied by SPEO (sample PS-g-SPEO-72.6).
},
	isbn = {10221336 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032352685\&partnerID=40\&md5=2d915a1de24f503ed01fc4c5ee85e639},
	author = {Ji, J. and Feng, L. and Qiu, Y. and Yu, X.}
}
