



@article {2342,
	title = {Comparative study of nanohydroxyapatite microspheres for medical applications},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {86},
	year = {2008},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31634PubMed ID: 17975824Language of Original Document: EnglishCorrespondence Address: Mateus, A. Y. P.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium hydroxide, 1305-62-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium, 7440-70-2; dodecyl sulfate sodium, 151-21-3; neutral red, 553-24-2; phosphoric acid, 7664-38-2; Biocompatible Materials; Durapatite, 1306-06-5; PowdersReferences: Jarcho, M., Calcium phosphates ceramics as hard tissue prosthetics (1981) Clin Orthop Relat Res, 157, pp. 259-278;Bucholz, R., Carlton, A., Holmes, R., Hydroxyapatite and tricalcium phosphate bone graft substitutes (1987) Orthop Clin N Am, 18, pp. 323-334; 
Daculsi, G., Passuti, N., Martins, S., Deudon, C., LeGeros, R., Raher, S., Macroporous calcium phosphate ceramic for ling bone surgery in humans and dogs: Clinical and histological study (1990) J Biomed Mater Res, 24, pp. 379-396; 
Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone in growth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Yuan, H., Kurashina, K., de Bruijn, J., Li, Y., de Groot, K., Zhang, X., A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics (1999) Biomaterials, 20, pp. 1799-1806; 
Liou, S., Chen, S., Liu, D., Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios (2003) Biomaterials, 24, pp. 3981-3988; 
Earl, J.S., Wood, D.J., Mile, S.J., Hydrothermal synthesis of hydroxyapatite (2006) J Phys Conf, 26, pp. 268-271; 
Han, J.K., Song, H.Y., Fumio, S., Lee, B.T., Synthesis of high purity nano-sized hydroxyapatite powder by microwave-hydrothermal method (2006) Mater Chem Phys, 99, pp. 235-239; 
Yoshimura, M., Sujaridworakun, P., Koh, F., Fujiwara, T., Pongkao, D., Ahniyaz, A., Hydrothermal conversion of calcite crystals to hydroxyapatite (2004) Mater Sci Eng C, 24, pp. 521-525; 
Bogdanoviciene, I., Beganskiene, A., Tonsuaadu, K., Glaser, J., Meyer, H.J., Kareiva, A., Calcium hydroxyapatite Ca 10(PO 4) 6 (OH) 2 ceramics prepared by aqueous sol-gel processing (2006) Mater Res Bull, 41, pp. 1754-1762; 
Liu, D.M., Troczynski, T., Tseng, W.J., Water-based sol-gel synthesis of hydroxyapatite: Process development (2001) Biomaterials, 22, pp. 1721-1730; 
Bezzi, G., Celotti, G., Landi, E., La Torretta, T.M.G., Sopyan, I., Tampieri, A., A novel sol-gel technique for hydroxyapatite preparation (2003) Mater Chem Phys, 78, pp. 816-824; 
An, G.H., Wang, H.J., Kim, B.H., Jeong, Y.G., Choa, Y.H., Fabrication and characterization of a hydroxyapatite nanopowder by ultrasonic spray pyrolysis with salt-assisted decomposition (2007) Mater Sci Eng A, pp. 449-451,821-824; 
Varma, H.K., Babu, S., Synthesis of calcium phosphate bioceramics by citrate gel pyrolisis method (2005) Ceram Int, 31, pp. 109-114; 
Jarudilokkula, S., Tanthapanichakoonb, W., Boonamnuayvittayaa, V., Synthesis of hydroxyapatite nanoparticles using an emulsion liquid membrane system Colloids Surf A, , Forthcoming; 
Bose, S., Saha, S.K., Synthesis and characterization of hydroxyapatite nanopowders by emulsion technique (2003) Chem Mater, 15, pp. 4464-4469; 
Nayar, S., Sihna, M.K., Basu, D., Sinha, A., Synthesis and sintering of biomimetic hydroxyapatite nanoparticles for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 1063-1068; 
Tas, A.C., Synthesis of biomimetic Ca-hydroxyapatite powders at 37{\textdegree}C in synthetic body fluids (2000) Biomaterials, 21, pp. 1429-1438; 
Wanga, A., Yina, H., Liua, D., Wub, H., Wadac, Y., Rena, M., Xua, Y., Chenga, X., Effects of organic modifiers on the size-controlled synthesis of hydroxyapatite nanorods (2007) Appl Surf Sci, 253, pp. 3311-3316; 
Zhang, H.G., Zhu, Q., Surfactant-assisted preparation of fluoride-substituted hydroxyapatite nanorods (2005) Mater Lett, 59, pp. 3054-3058; 
Liu, Y., Houa, D., Wangb, G., A simple wet chemical synthesis and characterization of hydroxyapatite nanorods (2004) Mater Chem Phys, 86, pp. 69-73; 
Pang, Y.X., Bao, X., Influence of temperature, ripening time and calcination on the morphology and crystallinity of hydroxyapatite nanoparticles (2003) J Eur Ceram Soc, 23, pp. 1697-1704; 
Gitzhofer, B.E., Boulos, M.I., Morpholgical study of hydroxyapatite nanocrystal suspension (2000) J Mater Sci: Mater Med, 11, pp. 523-531; 
Barbosa, M., Granja, P., Barrias, C., Amaral, I., Polysaccharides as scaffolds for bone regeneration (2005) ITBM-RBM, 26, pp. 212-217; 
Hari, P., Chandy, T., Sharma, C., Chitosan/calcium-alginate beads for oral delivery of insulin (1996) J Appl Polym Sci, 59, pp. 1796-1801; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Key Eng Mater, pp. 254-262,877-880; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 455-463; 
Bowersock, T., Martins, S., Vaccine delivery to animals (1999) Adv Drug Deliv Rev, 32, pp. 167-194; 
Kong, H., Smith, M., Mooney, D., Designing alginate hydrogels to maintain viability of immobilized cells (2003) Biomaterials, 24, pp. 4023-4029; 
Legallais, C., David, B., Dor{\'e}, E., Bioartificial livers (BAL): Current technological aspects and future developments (2001) J Membr Sci, 181, pp. 81-95; 
Liu, X.D., Yu, W.Y., Zhang, Y., Xue, W.M., Yu, W.T., Xiong, Y., Ma, X.J., Yuan, Q., Characterization of structure and diffusion behaviour of Ca-alginate beads prepared with external or internal calcium sources (2002) J Microencapsul, 19 (6 -1), pp. 775-782; 
Taqieddin, E., Amiji, M., Enzyme immobilization in novel alginate-chitosan coreshell microcapsules (2004) Biomaterials, 25, pp. 1937-1945; 
Ribeiro, C., Barrias, C., Barbosa, M., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Kim, Y.J., Park, H.G., Yang, Y.L., Yoon, Y., Kim, S., Oh, E., Multifunctional drug delivery system using starch-alginate beads for controlled release (2005) Biol Pharm Bull, 28, pp. 394-397; 
Barrias, C., Ribeiro, C., Lamghari, M., Sa, C., Barbosa, M., Proliferation, activity and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) J Biomed Mater Res A, 72, pp. 57-66; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration (2007) Key Eng Mater, pp. 330-332,243-246; 
Mankani, M., Fowler, B., Kingman, A., Robey, P., In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape (2001) Biotechonol Bioeng, 72, pp. 96-107; 
Higashi, T., Okamoto, H., Influence of particle size of calcium phosphate ceramics as a capping agent on the formation of a hard tissue barrier in amputated dental pulp (1996) J Endodoncy, 22, pp. 281-283; 
Guo, G., Xun, Y., Wnag, Z., Guo, H., Preparation of hydroxyapatite nanoparticles by reverse microemulsion (2005) Ceram Int, 331, pp. 869-872; 
Sun, X.M., Chen, X., Deng, Z.X., Li, Y.D., A CTAB-assited hydrothermal orientation growth of ZnO nanorods (2002) Mater Chem Phys, 78, pp. 99-104; 
Uota, M., Arakawa, H., Kitamura, N., Yoshimura, T., Tanaka, J., Kijima, T., Synthesis of high surface area hydroxyapatite nanoparticles by mixed surfactant-mediated approach (2005) Langmuir, 21, pp. 4724-4728; 
Balasundaram, G., Sato, M., Webster, T.J., Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD (2006) Biomaterials, 27, pp. 2798-2805; 
Wutticharoenmongkol, P., Sanchavanakit, N., Pavasant, P., Supaphol, P., Novel bone scaffolds or electrospun polycaprolactone fibers filled with nanoparticles (2006) J Nanosci Nanotechnol, 6, pp. 514-522; 
Gonzalez-McQuire, R., Green, D., Walsh, D., Hall, S., Chane-Ching, J., Oreffo, R.O.C., Mann, S., Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating (2005) Biomaterials, 26, pp. 6652-6656; 
Sun, W.B., Wu, Y.F., Ding, Y., Chu, C.L., Liu, T.J., The expression and activity of alkaline phosphatase in human periodontal ligament cells with nanometer hydroxyapatite (2006) Zhonghua Kou Qiang Yi Xue Za Zhi, 41, pp. 348-349; 
Bowers, K., Keller, J.C., Randolph, B., Wick, D., Michaels, C., Optimization of surface micromorphology for enhanced osteoblast responses in vitro (1992) Int J Oral Maxillofac Impl, 7, pp. 302-310; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford Jr, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401; 
Boyan, B.D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D.L., Szmuckler-Monclers, S., Dean, D.D., Schwartz, Z., Titanium surface roughness alters responsiveness of MG63 osteoblastic-like cells to 1α,25-(OH) 2D 3 (1998) J Biomed Mater Res, 39, pp. 77-85
},
	month = {2008///},
	pages = {483 - 493},
	abstract = {This study concerns the preparation, physical, and in vitro characterization of two different types of hydroxyapatite (HA) microspheres, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using the chemical precipitation synthesis with H                        3PO
                        4, Ca(OH) 
                        2, and a surfactant, SDS (sodium dodecyl sulfate), as starting reagents. The HA powders were dispersed in a sodium alginate solution, and spherical particles were obtained by droplet extrusion coupled with ionotropic gelation in the presence of Ca
                        2+. These were subsequently sintered to produce HA-1 and HA-2 microspheres with a uniform size and interconnected microporosity. Both powders and microspheres were characterized using FTIR and X-ray diffraction. Moreover, SEM and mercury intrusion porosimetry were used to analyze the microspheres, and TEM was used to analyze the powders. Results showed that pure HA and mixtures of HA/β-TCP in the nanometer range and needlelike shape were obtained for HA-1 and HA-2 powders, respectively. Neutral Red, scanning electron microscopy and confocal microscopy were used to evaluate the behavior of osteoblastic-like MG-63 cells cultured on HA microspheres surfaces for 7 days. Results showed that good adhesion and proliferation of osteoblasts on the HA microspheres surface. Cells built bridges between adjacent microspheres, forming microspheres-cells clusters in both types of materials. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {alginic acid, analytic method, Apatite, article, Biocompatible Materials, Biological materials, biomaterial, Bone Regeneration, Calcium, calcium hydroxide, calcium phosphate, cell adhesion, Cell Line, cell proliferation, Chemical precipitation, Chemotherapy, Comparative studies, comparative study, controlled study, dodecyl sulfate sodium, Drug delivery, Drug delivery system (DDS), Drug Delivery Systems, Drug dosage, Durapatite, Gelation, human, human cell, Humans, hydroxyapatite, Hydroxyapatite (HAP), Hydroxylation, In vitro characterization, in vitro study, infrared spectroscopy, materials testing, Medical applications, Mercury intrusion porosimetry, microsphere, Microspheres, Nano-hydroxyapatite (n-HA), Nanohydroxyapatite, nanoparticle, nanoparticles, neutral red, osteoblast, Osteoblasts, Particle Size, phosphoric acid, porosity, powder, Powders, precipitation, Precipitation (chemical), reagent, Regeneration (renovating), Scanning electron microscopy, surfactant, synthesis, Synthesis (chemical), X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47049103112\&partnerID=40\&md5=138b3e2f3a97d8b398546d3f85567a0f},
	author = {Mateus, A.Y.P. and Barrias, C.C. and Ribeiro, C. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2527,
	title = {Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima: A new insight in the biomineralization field},
	journal = {European Journal of BiochemistryEur. J. Biochem.},
	volume = {269},
	year = {2002},
	note = {Cited By (since 1996): 58Export Date: 21 November 2012Source: ScopusCODEN: EJBCAdoi: 10.1046/j.1432-1033.2002.03203.xPubMed ID: 12383258Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiologie Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Amino Acids; Calcium, 7440-70-2; Edetic Acid, 60-00-4; Extracellular Matrix Proteins; Glycosaminoglycans; Insect Proteins; SilkReferences: Crenshaw, M.A., The soluble matrix from Mercenaria mercenaria shell (1972) Biomineralization, 6, pp. 6-11;Krampitz, G., Engels, J., Cazaux, C., Biochemical studies on water-soluble proteins and related components of gastropod shells (1976) The Mechanisms of Mineralization in the Invertebrates and Plants, pp. 175-190. , Watabe, N. \& Wilbur, K.M., eds. The Barush B.W. Library in Marine Science no. 5, The University of South Carolina Press, Columbia; 
Weiner, S., Traub, W., Lowenstam, H.A., Organic matrix in calcified exoskeletons (1983) Biomineralization and Biological Metal Accumulation, pp. 205-224. , Westbroek, P. \& deJong, E.W., eds. Reidel, Dordrecht, Holland; 
Keith, J., Stockwell, D., Ball, D., Remillard, K., Kaplan, D., Thannhauser, T., Sherwood, R., Comparative analysis of macromolecules in mollusc shells (1993) Comp. Biochem. Physiol., 105 B, pp. 487-496; 
Levi-Kalisman, Y., Falini, G., Addadi, L., Weiner, S., Structure of the nacreous organic matrix of a bivalve mollusk shell examined in the hydrated state using cryo-TEM (2001) J. Struct. Biol., 135, pp. 8-17; 
Weiner, S., Hood, L., Soluble protein of the organic matrix of mollusk shells: A potential template for shell formation (1975) Science, 190, pp. 987-989; 
Weiner, S., Traub, W., Macromolecules in mollusc shells and their functions in biomineralization (1984) Phil. Trans. R. Soc. Lond. B, 304, pp. 425-434; 
Belcher, A.M., Wu, X.H., Christensen, R.J., Hansma, P.K., Stucky, G.D., Morse, D.E., Control of crystal phase switching and orientation by soluble mollusc-shell proteins (1996) Nature, 381, pp. 56-58; 
Kamat, S., Su, X., Ballarini, R., Heuer, A.H., Structural basis for the fracture toughness of the shell of the conch Strombus gigas (2000) Nature, 405, pp. 1036-1040; 
Hare, P.E., Amino acids in the proteins from aragonite and calcite in the shells of Mytilus californianus (1963) Science, 139, pp. 216-217; 
Hare, P.E., Abelson, P.H., Amino acid composition of some calcified proteins (1965) Carnegie Inst. Washington Year Book, 64, p. 223; 
Watabe, N., Shell (1984) Biology of the Integument, 1, pp. 448-485. , Invertebrates (Bereiterhahn, J., Matolsty, A.G. \& Richards, K.S., eds). Springer-Verlag, Berlin; 
Mann, S., Molecular recognition in biomineralization (1988) Nature, 332, pp. 119-124; 
Kono, M., Hayashi, N., Samata, T., Molecular mechanism of the nacreous layer formation in Pinctada maxima (2000) Biochem. Biophys. Res. Commun., 269, pp. 213-218; 
Lopez, E., Berland, S., Le Faou, A., Nacre, osteogenic and osteoinductive properties (1994) Bull. Inst. Oc{\'e}an Monaco, 14, pp. 49-57; 
Lopez, E., Vidal, B., Berland, S., Camprasse, C., Camprasse, G., Silve, C., Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro (1992) Tissue Cell, 24, pp. 667-679; 
Atlan, G., Balmain, N., Berland, S., Vidal, B., Lopez, E., Reconstruction of human maxillary defects with nacre powder: Histological evidence for bone regeneration (1997) C. R. Acad. Sci. Paris, Life Sci., 320, pp. 253-258; 
Atlan, G., Delattre, O., Berland, S., Le Faou, A., Nabias, G., Cot, D., Lopez, E., Interface between bone and nacre implants in sheep (1999) Biomaterials, 20, pp. 1017-1022; 
Lamghari, M., Almeida, M.J., Berland, S., Huet, H., Laurent, A., Milet, C., Lopez, E., Stimulation of bone marrow cells and bone formation by nacre: In vivo and in vitro studies (1999) Bone, 25 S, pp. 91S-94S; 
Almeida, M.J., Milet, C., Peduzzi, J., Pereira, L., Haigle, J., Barth{\'e}lemy, M., Lopez, E., The effect of water-soluble matrix fraction extracted from the nacre of Pinctada maxima on the alkaline phosphatase activity of cultured fibroblasts (2000) J. Exp. Zool. (Mol. Dev. Evol.), 288, pp. 327-334; 
Almeida, M.J., Pereira, L., Milet, C., Haigle, J., Barbosa, M., Lopez, E., Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts (2001) J. Biomed. Mater. Res., 57, pp. 306-312; 
Lamghari, M., Berland, S., Laurent, A., Huet, H., Lopez, E., Bone reactions to nacre injected percutaneously into the vertebrae of sheep (2001) Biomaterials, 22, pp. 555-562; 
Lopez, E., Giraud, M., Le Faou, A., Berland, S., Gutierrez, J., (1995), Proc{\'e}d{\'e} de pr{\'e}paration de substances actives {\`a} partir de la nacre, produits obtenus, utiles comme m{\'e}dicaments. Patent CNRS no. FR. 9515650, 28/12/1995, WO 97/24133-10/07/97Wheeler, A.P., Rusenko, K.W., George, J.W., Sikes, S., Evaluation of calcium binding by molluscan shell organic matrix and its relevance to biomineralization (1987) Comp. Biochem. Physiol., 87 B, pp. 953-960; 
Cohen-Solal, L., Lian, J.B., Kossiva, D., Glimcher, M.J., The identification of O-phosphothreonine with soluble non-collagenous phosphoproteins of bone matrix (1978) FEBS Lett., 89, pp. 107-110; 
Cohen, S.A., Strydom, D.J., Amino acid analyses utilizing phenylisothiocyanate derivatives (1988) Anal. Biochem., 174, pp. 1-16; 
Hewitt, B.R., Spectrophotometric determination of protein in alkaline solution (1958) Nature, 187, p. 246; 
Whiteman, P., The quantitative determination of glycosaminoglycans in urine with Alcian blue 8GX (1973) Biochem. J., 131, pp. 351-357; 
Whiteman, P., The quantitative measurement of Alcian blue-glycosaminoglycan complexes (1973) Biochem. J., 131, pp. 343-350; 
Gold, E.W., A simple spectrophotometric method for estimating glycosaminoglycan concentrations (1979) Anal. Biochem., 99, pp. 183-188; 
Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685; 
Morrissey, J.H., Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity (1981) Anal. Biochem., 117, pp. 307-310; 
Wheeler, A.P., Rusenko, K.W., Sikes, S., Organic matrix from carbonate biomineral as a regulator of mineralization (1988) Chemical Aspects of Regulation of Mineralization, , (Sikes, C.S. \& Wheeler, A.P., eds). University of South Alabama Publication Services, Mobile, Alabama; 
Weiner, S., Aspartic acid-rich proteins: Major components of the soluble organic matrix of mollusk shells (1979) Calcif. Tissue Int., 29, pp. 163-167; 
Wheeler, A.P., Rusenko, K.W., Swift, D.M., Sikes, S., Regulation of in vitro and in vivo CaCO 3 crystallization by fractions of oyster shell organic matrix (1988) Mar. Biol., 98, pp. 71-80; 
Halloran, B.A., Donachy, J.E., Characterization of organic matrix macromolecules from the shells of the antarctic scallop Adamussium colbecki (1995) Comp. Biochem. Physiol., 111 B, pp. 221-231; 
Scott, J.E., Quintarelli, G., Dellovo, M.C., The chemical and histochemical properties of Alcian blue staining (1964) Histochemie, 4, pp. 73-85; 
Nakamoto, K., (1986) IR and Raman Spectra of Inorganic and Coordination Compounds, , J. Wiley \& Sons, New York; 
Samata, T., Krampitz, G.P., Calcium binding polypeptides in oyster shells (1982) Malacologia, 22, pp. 225-233; 
Roeges, N.P.G., (1998) A Guide to the Complete Interpretation of Infrared Spectra of Organic Structures, , J. Wiley \& Sons, New York; 
Cariolou, M., Morse, D., Purification and characterization of calcium binding conchiolin shell peptides from the mollusc, Haliotis rufescens, as a function of development (1988) J. Comp. Physiol., 157 B, pp. 717-729; 
Krampitz, G.P., Structure of the organic matrix in mollusc shells and avian eggshells (1982) Biological Mineralization and Demineralization, pp. 219-232. , Nancollas, G.H., ed. Springer-Verlag, Berlin, Heidelberg, New York; 
Weiner, S., Addadi, L., Acidic macromolecules of mineralized tissues: The controllers of crystal formation (1991) Trends Biochem. Sci., 16, pp. 252-256; 
Marin, F., Pereira, L., Westbroek, P., Large-scale fractionation of molluscan shell matrix (2001) Protein Expr. Purif., 23, pp. 175-179; 
Balmain, J., Hannoyer, B., Lopez, E., Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analyses of mineral and organic matrix during heating of mother of pearl (nacre) from the shell of the mollusc Pinctada maxima (1999) J. Biomed. Mater. Res. Appl. Mat., 48, p. 754; 
Marxen, J.C., Hammer, M., Gehrke, T., Becker, W., Carbohydrates of the organic shell matrix and the shell forming tissue of the snail Biomphalaria glabrata (Say) (1998) Biol. Bull., 194, pp. 231-240; 
Miyazawa, T., Blout, E.R., The infrared spectra of polypeptides in various conformations - Amide I and II bands (1961) J. Am. Chem. Soc., 83, pp. 712-719; 
Worms, D., Weiner, S., Mollusk shell organic matrix: Fourier transform infrared study of the acidic macromolecules (1986) J. Exp. Zool., 237, pp. 11-20; 
Weiner, S., Traub, W., X-ray diffraction study of the insoluble organic matrix of mollusk shells (1980) FEBS Lett., 111, pp. 311-316; 
Pereira-Mouri{\`e}s, L., Almeida, M.J., Marin, F., Gay, M., Milet, C., Lopez, E., Soluble proteins of the nacre from the giant oyster Pinctada maxima with highly hydrophobic amino acids (2002) Biomineralization: Formation, Diversity, Evol. Appl., , in press; 
Nakahara, H., Kakei, M., Bevelander, G., Fine structure and amino acid composition of the organic {\textquoteright}envelope{\textquoteright} in the prismatic layer of some bivalve shells (1980) Venus Jpn. J. Malacol., 39, pp. 167-177; 
Falini, G., Albeck, S., Weiner, S., Addadi, L., Control of aragonite or calcite polymorphism by mollusk shell macromolecules (1996) Science, 271, pp. 67-69; 
Simkiss, K., The organic matrix of the oyster shell (1965) Comp. Biochem. Physiol., 16, pp. 427-435; 
Golberg, M., Takagi, M., Dentine proteoglycans: Composition, ultrastructure, and functions (1993) Histochem. J., 25, pp. 781-806; 
Wilbur, K.M., Recent studies of invertebrate mineralization (1976) The Mechanisms of Mineralization in Invertebrates and Plants, pp. 79-108. , Watabe, N. \& Wilbur, K.M., eds. The University of South Carolina Press, The Belle W. Baruch Library in Marine Science no. 5, Columbia; 
Addadi, L., Moradian, J., Shay, E., Maroudas, N.G., Weiner, S., A chemical model for the cooperation of sulfates and carboxylates in calcite crystal nucleation: Revelance to biomineralization (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 2732-2736; 
Marxen, J.C., Becker, W., Calcium binding constituents of the organic shell matrix from the freshwater snail Biomphalaria glabrata (2000) Comp. Biochem. Physiol., 127 B, pp. 235-242; 
Tanaka, Y., Adams, D.H., Shaw, S., Proteoglycans on endothelial cells present adhesion-inducing cytokines to leukocytes (1993) Immunol. Today, 14, pp. 111-115; 
Jackson, R.L., Bush, S.J., Cardin, A.D., Glycosaminoglycans: Molecular properties, protein interactions, and role in physiological processes (1991) Physiol. Rev., 71, pp. 481-539; 
Nakahara, H., Calcification of gastropod nacre (1983) Biomineralization and Biological Metal Accumulation, pp. 225-230. , Westbroek, P. \& deJong, E.W., eds. Reidel, Dordrecht, Holland; 
Zentz, F., B{\'e}douet, L., Almeida, M.J., Milet, C., Lopez, E., Giraud, M., Characterization and quantification of chitosan extracted from nacre of the abalone Haliotis tuberculata and the oyster Pinctada maxima (2001) Mar. Biotechnol., 3, pp. 36-44; 
Alzieu, C., Thibaud, Y., Heral, M., Boutier, B., Evaluation des risques dus a l{\textquoteright}emploi des peintures anti-sallissures dans les zones conchyoliques (1980) Rev. Trav. Inst. P{\^e}ches Marit., 44, pp. 301-348; 
Alzieu, C., Heral, M., Thibaud, Y., Dardignac, M.J., Feuillet, M., Influence des peintures anti-sallissures {\`a} base d{\textquoteright}organostanniques sur la calcification de la coquille de l{\textquoteright}hu{\^\i}tre Crassostrea gigas (1982) Rev. Trav. Inst. P{\^e}ches Marit., 45, pp. 101-116; 
Krampitz, G., Drolshagen, H., Deltreil, J.P., Soluble matrix components in malformed oyster shells (1983) Experientia, 39, pp. 1105-1106; 
Alzieu, C., Heral, M., Ecotoxicological effects of organotin coumpounds on oyster culture (1984) Ecotoxicological Testing for the Marine Environment, 2, pp. 187-196. , Persoone, G., Jaspers, E. \& Claus, C., eds. State University Ghent and Inst. Mar. Sci. Res., Bredene, Belgium; 
Almeida, M.J., (1996) Crescimento em Ostras: Factores que Afectam a Forma{\c c}{\~a}o e Estrutura da Concha, , PhD Thesis, Instituto de Ci{\^e}ncias Biom{\'e}dicas Abel Salazar, University of Porto, Portugal; 
Sudo, S., Fujikawa, T., Nagakura, T., Ohkubo, T., Sakaguchi, K., Tanaka, M., Nakashina, K., Takahashi, T., Structures of mollusc shell framework proteins (1997) Nature, 387, pp. 563-564; 
Shen, X., Belcher, A.M., Hansma, P.K., Stucky, G.D., Morse, D.E., Molecular cloning and characterization of Lustrin A, a matrix protein from shell and pearl nacre of Haliotis rufescens (1997) J. Biol. Chem., 272, pp. 32472-32481; 
Samata, T., Hayashi, N., Kono, M., Hasegawa, K., Horita, C., Akera, S., A new matrix protein family related to the nacreous layer formation of Pinctada fucata (1999) FEBS Lett., 462, pp. 225-229; 
Mann, S., Webb, J., Williams, R.J.P., (1989) Biomineralization: Chemical and Biochemical Perspectives, pp. 35-62. , VCH, Weinheim; 
Simkiss, K., Wilbur, K.M., (1989) Biomineralization - Cell Biology and Mineral Deposition, , Academic Press, San Diego; 
Sarashina, I., Endo, K., Primary structure of a soluble matrix protein of scallop shell: Implications for calcium carbonate biomineralization (1998) Am. Mineral., 83, pp. 1510-1515; 
Weiss, I., Kaufmann, S., Mann, K., Fritz, M., Purification and characterization of perlucin and perlustrin, two new proteins from the shell of the mollusc Haliotis laevigata (2000) Biochem. Biophys. Res. Commun., 267, pp. 17-21; 
Pereira-Mouri{\`e}s, L., Milet, C., Almeida, M.J., Rousseau, M., Borzeix, S., Lopez, E., Biological effect of the water-soluble matrix extract of the nacre shell layer of Pinctada maxima on vertebrate cell proliferation and differentiation (2001) Proceedings of the 3rd International Satellite Symposium on the Comparative Endocrinology of Calcium Regulation, , http://www.sci.port.ac.uk/-cecr, Gay, C.V., Dacke, C.G., Dancks, J.A. \& Cox, P.A., eds; 
Pereira-Mouri{\`e}s, L., Almeida, M.J., Milet, C., Berland, S., Lopez, E., Bioactivity of nacre water-soluble organic matrix from the bivalve mollusk Pinctada maxima in three mammalian cell types: Fibroblasts, bone marrow stromal cells and osteoblasts (2002) Comp. Biochem. Physiol., 132, pp. 215-217
},
	month = {2002///},
	pages = {4994 - 5003},
	abstract = {Nacre organic matrix has been conventionally classified as both {\textquoteright}water-soluble{\textquoteright} and {\textquoteright}water-insoluble{\textquoteright}, based on its solubility in aqueous solutions after decalcification with acid or EDTA. Some characteristics (aspartic acid-rich, silk-fibroin-like content) were specifically attributed to either one or the other. The comparative study on the technique of extraction (extraction with water alone vs. demineralization with EDTA) presented here, seems to reveal that this generally accepted classification may need to be reconsidered. Actually, the nondecalcified soluble organic matrix, extracted in ultra-pure water, displays many of the characteristics of what until now has been called {\textquoteright}insoluble matrix{\textquoteright}. We present the results obtained on this extract and on a conventional EDTA-soluble matrix, with various characterization methods: fractionation by size-exclusion and anion-exchange HPLC, amino acid analysis, glycosaminoglycan and calcium quantification, SDS/PAGE and FTIR spectroscopy. We propose that the model for the interlamellar matrix sheets of nacre given by Nakahara [In: Biomineralization and Biological Metal Accumulation, Westbroek, P. \& deJong, E.W., eds, (1983) pp. 225-230. Reidel, Dordrecht, Holland] and Weiner and Traub [Phil. Trans. R. Soc. Lond. B (1984) 304, 425-434] may no longer be valid. The most recent model, proposed by Levi-Kalisman et al. [J. Struct. Biol. (2001) 135, 8-17], seemed to be more in accordance with our findings.},
	keywords = {amino acid analysis, Amino acids, Animals, anion exchange chromatography, article, Biochemistry, biomineralization, bivalve, Bivalvia, Calcium, Chemical Fractionation, Chromatography, High Pressure Liquid, controlled study, demineralization, edetic acid, EDTA-soluble matrix, Electrophoresis, Polyacrylamide Gel, Extracellular Matrix Proteins, extraction, fractionation, gel permeation chromatography, glycosaminoglycan, Glycosaminoglycans, High performance liquid chromatography, hydrophobicity, infrared spectroscopy, Insect Proteins, model, Nacre, nonhuman, Ostreidae, Pinctada, Pinctada maxima, polyacrylamide gel electrophoresis, priority journal, Silk, Silk-fibroin-like-proteins, Solubility, Spectroscopy, Fourier Transform Infrared, Undecalcified soluble matrix, Water},
	isbn = {00142956 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036408881\&partnerID=40\&md5=7e99def46be711f02de5f4dd3dc8f63e},
	author = {Pereira-Mouri{\`e}s, L. and Almeida, M.-J. and Ribeiro, C. and Peduzzi, J. and Barth{\'e}lemy, M. and Milet, C. and Lopez, E.}
}
@article {2615,
	title = {Amino acid and metal content of Crassostrea gigas shell infested by Polydora sp. in the prismatic layer insoluble matrix and blister membrane},
	journal = {Aquatic Living ResourcesAquatic Living Resour.},
	volume = {9},
	year = {1996},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: ALREELanguage of Original Document: EnglishCorrespondence Address: Almeida, M.J.; Laborat{\'o}rio de Fisiologia Aplicada, Instituto de Ci{\^e}ncias Biom{\'e}dicas Abel Salazar, 4050 Porto, PortugalReferences: Allen, J.A., Manganese deposition on the shell of living molluscs (1960) Nature, 185, pp. 336-337;Beedham, G.E., Repair of the shell in species of Anodonta (1965) Proc. Zool. Soc. London, 145, pp. 107-125; 
Boggess, R.K., Martin, R.B., Copper chelation by DOPA, epinephrine and other catechols (1975) J. Am. Soc. Chem., 97, pp. 3076-3081; 
Brown, C.H., (1975) Structural Materials in Animals, , Wiley, New York; 
Bubel, A., An electron microscope study of periostracum repair in Mytilus edulis (1973) Mar. Biol., 20, pp. 235-244; 
Carriker, M.R., Swann, C.P., Prezant, R.S., Counts, C.L., Chemical elements in the aragonitic and calcitic microstructural groups of shell of the oyster Crassostrea virginica: A proton probe study (1991) Mar. Biol., 109, pp. 289-297; 
Catherine, M., Blateau, D., Mazuri{\'e}, J., Le Bec, C., (1990) Anomalies des Coquilles D{\textquoteright}hu{\^\i}tres Creuses Crassostrea Gigas Observ{\'e}es sur le Littoral Fran{\c c}ais en Mai-juin 1989, Dues au ver Polydora et aux Peintures Antisalissures., pp. 13-14. , Rapp. Int. Dir. Ressources Vivantes, IFREMER Nantes; 
Degens, E.T., Spencer, D.W., Parker, R.H., Paleobiochemistry of Molluscan shell proteins (1967) Comp. Biochem. Physiol., 79, pp. 553-579; 
Dorsett, D.A., The behavior of Polydora ciliata (Johnst.). Tube building and burrowing (1961) J. Mar. Biol. Assoc. U.K., 41, pp. 577-590; 
Felix, C.C., Sealy, R.C., Electron spin resonance characterization of radicals from 3-4-dihydroxyphenylalanine: Semiquinone anions and their metal chelates (1981) J. Am. Chem. Soc., 103, pp. 2831-2835; 
Galstoff, P.S., The American oyster Crassostrea virginica (Gmelin) (1964) Fish. Bull. U.S., p. 64; 
Gergely, A., Kiss, T., Coordination chemistry of L-DOPA and related ligands (1979) Met. Ions Biol. Syst., 9, pp. 143-172; 
Haigler, S., Boring mechanism of Polydora websteri inhabiting Crassostrea virginica (1969) Am. Zool., 9, pp. 821-828; 
Jones, G.M., Saleuddin, A.S.M., Cellular mechanisms of periostracum formation in Physa spp. (Mollusca: Pulmonata) (1978) Can. J. Zool., 56, pp. 2299-2311; 
Kawaguchi, T., Watabe, N., The organic matrices of the shell of the American oyster Crassostrea virginica Gmelin (1993) J. Exp. Mar. Biol. Ecol., 170, pp. 11-28; 
Kawaguti, S., Ikemoto, N., Electron microscopy on the mantle of a bivalve Musculus senhousia, during regeneration of shell (1962) Biol. J. Okayama Univ., 8, pp. 31-42; 
Krampitz, G., Drolshagen, H., Hotta, S., Simultaneous binding of calcium and bicarbonate by conchiolin of oyster shells (1983) Experiemia, 39, pp. 1104-1105; 
Laukner, G., Diseases of Mollusca: Bivalvia (1983) Diseases of Marine Animals, Vol. II, Bivalvia to Scaphopoda, 2, pp. 477-978. , Biologische Anstalt Helgoland, O. Kinne ed. Hamburg; 
Levitt, M., A simplified representation of protein conformation for rapid simulation of protein folding (1976) J. Mol. Biol., 104, pp. 59-108; 
Linderstrom-Lang, K., Duspiva, F., Studies in enzymatic histochemistry. XVI. the digestion of keratin by larvae of clothes moth (Tineola biselliela Humm.) (1936) C. R. Trav. Lab. Carlsberg, Ser. Chim., 21, pp. 1-82; 
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein determination with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; 
Martius, K.V., Schwarzhans, K.E., Preparation of the Mn (II) complex with 3,4-dihydroxyphenylalanine (DOPA) (1978) Z Naturforsch., 33 B, p. 124; 
Meenaakshi, V.R., Hare, P.E., Watabe, N., Wilbur, K.M., The chemical composition of the periostracum of the molluscan shell (1969) Comp. Biochem. Physiol., 29, pp. 611-620; 
Nozaki, Y., Tanford, C., The solubility of amino acids and two glycyl peptides in aqueous ethanol and dioxane solutions (1971) J. Biol. Chem., 246, pp. 2211-2217; 
Saleuddin, A.S.M., Petit, H.P., The mode of formation and the structure of the periostracum (1983) The Mollusca, 4, pp. 199-234. , K. M. Wilbur ed. Academic Press, New York; 
Sato-Okoshi, W., Okoshi, K., Microstructure of scallop and oyster shells infested with boring Polydora (1993) Nippon Suisan Gakkaishi, 59, pp. 1243-1247; 
Segar, D.A., Collins, J.D., Riley, J.P., The distribution of the major and some minor elements in marine animals. Part II (1971) Molluscs. J. Mar. Biol. Assoc. U.K., 51, pp. 131-136; 
Swinehart, J.H., Smith, K.W., Iron and manganese deposition in the periostraca of several bivalve molluscs (1979) Biol. Bull., 156, pp. 369-381; 
Travis, D.F., Gonsalves, M., Comparative ultrastructure and organization of the prismatic region of two bivalves and its possible relation to the chemical mechanism of boring (1969) Am. Zool., 9, pp. 635-661; 
Tsujii, T., An electron microscopic study of the mantle epithelial cells of Anodonta sp. during shell regeneration (1976) The Mechanisms of Mineralization in the Invertebrates and Plants, pp. 339-353. , N. Watabe, K. M. Wilbur eds. Univ. South Carolina Press, Columbia; 
Vovelle, J., Scl{\'e}rotisation et min{\'e}ralisation des structures squellettiques chez les Mollusques (1974) Haliotis, 2, pp. 133-165; 
Waite, J.H., Evidence for the mode of sclerotization in a molluscan periostracum (1977) Comp. Biochem. Physiol., 58 B, pp. 157-162; 
Waite, J.H., Quinone-tanned scleroproteins (1983) The Mollusca, 1, pp. 467-504. , K. M. Wilbur ed. Academic Press, New York; 
Waite, J.H., Andersen, S.O., 3,4-dihydroxyphenyl-alanine in an insoluble shell protein of Mytilus edulis (1978) Biochem. Biophys. Acta, 541, pp. 107-114; 
Wheeler, A.P., Rusenko, K.W., Swift, D.M., Sikes, C.S., Regulation of in vitro and in vivo CaCO 3 crystallization by fractions of oyster shell organic matrix (1988) Mar. Biol., 98, pp. 71-80; 
Zottoli, R.A., Carriker, M.R., Burrow morphology, tube formation and microarchitecture of shell dissolution by the spionid polychaete Polydora websteri (1974) Mar. Biol., 27, pp. 307-316
},
	month = {1996///},
	pages = {179 - 186},
	abstract = {The spionid polychaete Polydora sp. can live and reproduce inside the oysters{\textquoteright} shell, excavating a U-shaped burrow the central portion of which is filled with detritus and particles of dissolved shell. The oyster responds by secreting an organic layer, confining the worm, and later covering it with calcitostracum. This process creates a characteristic "mud blister". Crassostrea gigas were analysed for the organic matrix amino acid content and 14 metals of the whole shell of normal oysters and severely Polydora infested ones. Amino acid, DOPA and FT-Raman analyses were also performed on isolated membranes from the characteristic mud blister and on prismatic layer insoluble matrix (PLIM) of infested oysters, with the aim of detecting any differences between the two organic matrices. Membranes and PLIM composition differ from whole normal shell organic matrix by having lower aspartate and glycine and higher levels of virtually all hydrophobic amino acids, especially alanine. There are no significant differences between membranes and PLIM in the ratio of charged to non-polar amino acids, respectively 0.54 and 0.59. L-DOPA content is also similar in the two matrices with 0.45 ({\textpm}0.18) and 0.57 ({\textpm}0.30) ng.mg},
	keywords = {Amino acids, Crassostrea gigas, Metals, Polydora sp., Shell organic matrix},
	isbn = {09907440 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0008495129\&partnerID=40\&md5=80ed4fe02d098797dec36a0481fbbecf},
	author = {Almeida, M.J. and Moura, G. and MacHado, J. and Coimbra, J. and Vilarinho, L. and Ribeiro, C. and Soares-Da-Silva, P.}
}
