



@article {2359,
	title = {Osteoblast adhesion and morphology on TiO                    2 depends on the competitive preadsorption of albumin and fibronectin
},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {84},
	year = {2008},
	note = {Cited By (since 1996): 34Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31201PubMed ID: 17607748Language of Original Document: EnglishCorrespondence Address: Sousa, S.R.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, R. do Campo Alegre, 823, 4150-180 Porto, Portugal; email: ssousa@ibmc.up.ptChemicals/CAS: fibronectin, 86088-83-7; human serum albumin, 9048-49-1; hydrogen peroxide, 7722-84-1; iodine 125, 14158-31-7, 22822-81-7; titanium dioxide, 1317-70-0, 1317-80-2, 13463-67-7, 51745-87-0; Albumins; Coloring Agents; Fibronectins; Iodine Radioisotopes; Neutral Red, 553-24-2; titanium dioxide, 13463-67-7; Titanium, 7440-32-6References: Anselme, K., Osteoblast adhesion on biomaterials (2000) Biomaterials, 21, pp. 667-681;Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkane-thiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Malmsten, M., Lassen, B., Competitive adsorption at hydrophobic surfaces from binary protein systems (1994) J Colloid Interface Sci, 166, pp. 490-498; 
Koenig, A.L., Gambillara, V., Grainger, D.W., Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates (2003) J Biomed Mater Res A, 64, pp. 20-37; 
Horbett, T.A., The role of adsorbed proteins in animal cell adhesion (1994) Colloids Surf B, 2, pp. 225-240; 
Huang, H.H., Ho, C.T., Lee, T.H., Lee, T.L., Liao, K.K., Chen, F.L., Effect of surface roughness of ground titanium on initial cell adhesion (2004) Biomol Eng, 21, pp. 93-97; 
Vitte, J., Benoliel, A.M., Pierres, A., Bongrand, P., Is there a predictable relationship between surface physical-chemical properties and cell behaviour at the interface? (2004) Eur Cell Mater, 7, pp. 52-63; 
Tsai, W.B., Grunkemeier, J.M., McFarland, C.D., Horbett, T.A., Platelet adhesion to polystyrene-based surfaces preadsorbed with plasmas selectively depleted in fibrinogen, fibronectin, vitronectin, or von Willebrand{\textquoteright}s factor (2002) J Biomed Mater Res, 60, pp. 348-359; 
Grainger, D.W., Pavon-Djavid, G., Migonney, V., Josefowicz, M., Assessment of fibronectin conformation adsorbed to polytetrafluoroethylene surfaces from serum protein mixtures and correlation to support of cell attachment in culture (2003) J Biomater Sci Polym Ed, 14, pp. 973-988; 
Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption on 18-alkyl chains immobilized on hydroxyl-terminated self-assembled monolayers (2005) Biomaterials, 26, pp. 3891-3899; 
Culp, L.A., Sukenik, C.N., Cell type-specific modulation of fibronectin adhesion functions on chemically-derivatized self-assembled monolayers (1998) J Biomater Sci Polym Ed, 9, pp. 1161-1176; 
Baugh, L., Vogel, V., Structural changes of fibronectin adsorbed to model surfaces probed by fluorescence resonance energy transfer (2004) J Biomed Mater Res A, 69, pp. 525-534; 
McClary, K.B., Ugarova, T., Grainger, D.W., Modulating fibroblast adhesion, spreading, and proliferation using self-assembled monolayer films of alkylthiolates on gold (2000) J Biomed Mater Res, 50, pp. 428-439; 
Sousa, S.R., Moradas-Ferreira, P., Melo, L.V., Saramago, B., Barbosa, M.A., Human serum albumin adsorption on TiO 2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Gehanno, V., Freitas, P.P., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Soares, J.C., Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Iodine-125. A Guide to Radioiodination Techniques. Little Chalfont, UK: Amersham Life Science; 1993. p 64Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
Sousa, S.R., Moradas-Ferreira, P., Barbosa, M., TiO 2 type influences fibronectin adsorption (2005) J Mater Sci Mater Med, 16, pp. 1173-1178; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., Attachment of cultured human bone cells to novel polymers (1999) J Biomed Mater Res, 44, pp. 1-11; 
McFarland, C.D., Thomas, C.H., DeFilippis, C., Steele, J.G., Healy, K.E., Protein adsorption and cell attachment to patterned surfaces (2000) J Biomed Mater Res, 49, pp. 200-210; 
Yang, Y.Z., Cavin, R., Ong, J.L., Protein adsorption on titanium surfaces and their effect on osteoblast attachment (2003) J Biomed Mater Res A, 67, pp. 344-349; 
MacDonald, D.E., Rapuano, B.E., Deo, N., Stranick, M., Somasundaran, P., Boskey, A.L., Thermal and chemical modification of titanium-aluminum-vanadium implant materials: Effects on surface properties, glycoprotein adsorption, and MG63 cell attachment (2004) Biomaterials, 25, pp. 3135-3146; 
Steele, J.G., Johnson, G., McFarland, C., Dalton, B.A., Gengenbach, T.R., Chatelier, R.C., Underwood, P.A., Griesser, H.J., Roles of serum vitronectin and fibronectin in initial attachment of human vein endothelial-cells and dermal fibroblasts on oxygen-containing and nitrogen-containing surfaces made by radiofrequency plasmas (1994) J Biomater Sci Polym Ed, 6, pp. 511-532; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res A, 66, pp. 247-259; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (1999) J Biomed Mater Res, 49, pp. 435-447; 
Webb, K., Hlady, V., Tresco, P.A., Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces (2000) J Biomed Mater Res, 49, pp. 362-368; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cell osteogenic differentiation and fibronectin adsorption on chitosan membranes: The effect of the degree of acetylation (2005) J Biomed Mater Res A, 75, pp. 387-397; 
Scotchford, C.A., Gilmore, C.P., Cooper, E., Leggett, G.J., Downes, S., Protein adsorption and human osteoblast-like cell attachment and growth on alkylthiol on gold self-assembled monolayers (2002) J Biomed Mater Res, 59, pp. 84-99; 
Pettit, D.K., Horbett, T.A., Hoffman, A.S., Influence of the substrate binding characteristics of fibronectin on corneal epithelial cell outgrowth (1992) J Biomed Mater Res, 26, pp. 1259-1275; 
Steele, J.G., Johnson, G., Underwood, P.A., Role of serum vitronectin and fibronectin in adhesion of fibroblasts following seeding onto tissue-culture polystyrene (1992) J Biomed Mater Res, 26, pp. 861-884
},
	month = {2008///},
	pages = {281 - 290},
	abstract = {This study aims at assessing the influence of the competitive preadsorption of human serum albumin (HSA) and human plasma fibronectin (FN) from binary solutions and 10\% plasma on MC3T3-E1 osteoblast adhesion and morphology on two types of TiO                        2 substrates. One was commercially pure titanium with a titanium oxide layer formed in an H
                        2O
                        2 solution and the other TiO
                        2 sputtered on Si (Sousa et al., Langmuir 2004; 20:9745-9754.). The strategy applied in the present investigation was to compare osteoblast adhesion to surfaces preadsorbed with HSA, FN, HSA/FN = 1, HSA/FN = 200, and 10\% plasma. The adsorption of proteins was evaluated measuring the amount and the effectiveness of binding with radiolabeled proteins, 
                        125I-FN and 
                        125I-HSA. Our results indicated that MC3T3-E1 osteoblast adhesion correlates well with the amounts of FN and HSA adsorbed on TiO
                        2 surfaces. Also, we found that fewer osteoblasts adhered to both substrates preadsorbed with HSA, HSA/FN = 200, and 10\% plasma, after 4 and 24 h, than to the surfaces preadsorbed with FN and HSA/FN = 1. For the latter, FN was able to compensate the inhibitory effect of HSA on osteoblast adhesion. Therefore, the presence of lower amounts of coadsorbed albumin may improve presentation of FN in a more integrin-recognized conformation, suggesting that some degree of molecular packing prevents loss of integrin-binding activity. FN reversibility does not seem to be dependent on the HSA/FN adsorption mass ratio in solution, suggesting that FN competitively adsorbs to TiO
                        2 in a favorable conformation and does not suffers subsequent conformational changes allowing exchange with other FN molecules in solution. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {2, 3T3 Cells, Adsorption, Albumins, animal cell, Animals, article, binding kinetics, cell adhesion, Coloring Agents, Competitive protein adsorption, controlled study, cytoskeleton, Data Interpretation, Statistical, Fibronectin, Fibronectins, FN, HSA, human serum albumin, hydrogen peroxide, iodine 125, Iodine Radioisotopes, isotope labeling, Mice, Microscopy, Confocal, mouse, neutral red, nonhuman, osteoblast, Osteoblast adhesion, Osteoblasts, Protein radiolabeling, Proteins, Radiology, Solutions, Sterilization, surface property, TiO, Titanium, Titanium dioxide},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38049002555\&partnerID=40\&md5=dd5a4dee91d3fe1e9eb9a6ba31553c0a},
	author = {Sousa, S.R. and Lamghari, M. and Sampaio, P. and Moradas-Ferreira, P. and Barbosa, M.A.}
}
@article {2381,
	title = {Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation},
	journal = {Journal of Biomaterials Science, Polymer EditionJ. Biomater. Sci. Polym. Ed.},
	volume = {18},
	year = {2007},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBSEEdoi: 10.1163/156856207780425068Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; Biocompatible Materials; Chitosan, 9012-76-4References: Vacanti, C.A., Bonassar, L.J., Vacanti, J.P., (2000) Principles of Tissue Engineering, p. 671. , R. P. Lanza, R. Langer and J. Vacanti Eds, p, Academic Press, San Diego, CA;Bruder, S.P., Caplan, A.I., (2000) Principles of Tissue Engineering, p. 683. , R. P. Lanza, R. Langer and J. Vacanti Eds, p, Academic Press, San Diego, CA; 
Burg, K.J.L., Porter, S., Kellam, J.F., (2000) Biomaterials, 21, p. 2347; 
Khor, E., Lim, L.Y., (2003) Biomaterials, 24, p. 2339; 
Nettles, D.L., Elder, S.H., Gilbert, J.A., (2002) Tissue Eng, 8, p. 1009; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., (2002) J. Control. Rel, 78, p. 187; 
Muzzarelli, R.A.A., (1997) Cell. Mol. Life Sci, 53, p. 131; 
Madihally, S.V., Matthew, H.W.T., (1999) Biomaterials, 20, p. 1133; 
Chupa, J.M., Foster, A.M., Sumner, S.R., Madihally, S.V., Matthew, H.W.T., (2000) Biomaterials, 21, p. 2315; 
Lahiji, A., Sohrabi, A., Hungerford, D.S., Frondoza, C.G., (2000) J. Biomed. Mater. Res, 51, p. 586; 
Ho, M.H., Wang, D.M., Hsieh, H.J., Liu, H.C., Hsien, T.Y., Lai, J.Y., Hou, L.T., (2005) Biomaterials, 26, p. 3197; 
A. Domard, in: Advances in Chitin Science, II, A. Domard, G. A. F. Roberts and K. M. Varum (Eds), p. 410. Jacques Andr{\'e}, Lyon (1997)Tomihata, K., Ikada, Y., (1997) Biomaterials, 18, p. 567; 
Baxter, A., Dillon, M., Taylor, K.D.A., Roberts, G.A.F., (1992) Int. J. Biol. Macromol, 14, p. 166; 
Anselme, K., (2000) Biomaterials, 21, p. 667; 
Prasitsilp, M., Jenwithisuk, R., Kongsuwan, K., Damrongchai, N., Watts, P., (2000) J. Mater. Sci. Mater. Med, 11, p. 773; 
Chatelet, C., Damour, O., Domard, A., (2001) Biomaterials, 22, p. 261; 
Dhiman, H.K., Ray, A.R., Panda, A.K., (2004) Biomaterials, 25, p. 5147; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., (2001) Polymer, 42, p. 3569; 
Mima, S., Miya, M., Iwamoto, R., Yoshikawa, S., (1983) J. Appl. Polym. Sci, 28, p. 1909; 
Aiba, S., (1986) Int. J. Biol. Macromol, 8, p. 173; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., (1996) Carbohydr. Polym, 29, p. 63; 
(1987) Guideline on Validation of the Limulus Amebocyte Lysate Test as an End-product Endotoxin Test for Human and Animal Parental Drugs, Biological Products, and Medical Devices, , US Department of Health and Human Services, Public Health Service, Food and Drug Administration, US Department of Health and Human Services, Washington, DC; 
Nakagawa, Y., Murai, T., Hasegawa, C., Hirata, M., Tsuchiya, T., Yagami, T., Haishima, Y., (2003) J. Biomed. Mater. Res, 66 B, p. 347; 
Walker, S.L., Bhattacharjee, S., Hoek, E.M.V., Elimelech, M., (2002) Langmuir, 18, p. 2193; 
Clover, J., Gowen, M., (1994) Bone, 15, p. 585; 
Mosmann, T., (1983) J. Immunol. Methods, 65, p. 55; 
Domke, J., Dannohl, S., Parak, W.J., Muller, O., Aicher, W.K., Radmacher, M., (2000) Colloid Surfaces B: Biointerfaces, 19, p. 367; 
Sorlier, P., Denuziere, A., Viton, C., Domard, A., (2001) Biomacromolecules, 2, p. 765; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., (2005) J. Biomed. Mater. Res, 75 A, p. 387; 
Andrade, J.D., Smith, L.M., Gregonis, D.E., (1985) Surface and Interfacial Aspects of Biomedical Polymers, 1, p. 249. , J. D. Andrade Ed, p, Plenum Press, New York, NY; 
Dewez, J.L., Doren, A., Schneider, Y.J., Rouxhet, P.G., (1999) Biomaterials, 20, p. 547; 
Kikuchi, T., Yoshikai, Y., Miyoshi, J., Katsuki, M., Musikacharoen, T., Mitani, A., Tanaka, S., Matsuguchi, T., (2003) J. Dent. Res, 82, p. 546; 
Denuziere, A., Ferrier, D., Damour, O., Domard, A., (1998) Biomaterials, 19, p. 1275; 
Shirosaki, Y., Tsuru, K., Hayakawa, S., Osaka, A., Lopes, M.A., Santos, J.D., Fernandes, M.H., (2005) Biomaterials, 26, p. 485; 
Wang, J.H., Wei, C.W., Liu, H.C., Young, T.H., (2003) J. Biomed. Mater. Res, 64 A, p. 606; 
Michael, K.E., Vernekar, V.N., Keselowsky, B.G., Meredith, J.C., Latour, R.A., Garcia, A.J., (2003) Langmuir, 19, p. 8033; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., (2003) J. Biomed. Mater. Res, 66 A, p. 247; 
Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., (2003) J. Biomed. Mater. Res, 67 A, p. 538; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., (1999) J. Biomed. Mater. Res, 44, p. 1; 
Cowles, E.A., Brailey, L.L., Gronowicz, G.A., (2000) J. Biomed. Mater. Res, 52, p. 725; 
Marco, R.A.W., Diaz-Montero, C.M., Wygant, J.N., Kleinerman, E.S., McIntyre, B.W., (2003) J. Cell. Biochem, 88, p. 1038; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Dean, D.D., Boyan, B.D., (1995) J. Biomed. Mater. Res, 29, p. 389; 
Anselme, K., Bigerelle, M., Noel, B., Dufresne, E., Judas, D., Iost, A., Hardouin, P., (2000) J. Biomed. Mater. Res, 49, p. 155; 
Zinger, O., Anselme, K., Denzer, A., Habersetzer, P., Wieland, M., Jeanfils, J., Hardouin, P., Landolt, D., (2004) Biomaterials, 25, p. 2695
},
	month = {2007///},
	pages = {469 - 485},
	abstract = {Chitosan (Ch) is being actively investigated as a non-protein template for the growth of an increasing number of anchorage-dependent cells, including chondrocytes and bone cells. In the present work, Ch films with degrees of N-acetylation (DAs) in the range of 4 to 49\% were evaluated with respect to the attachment, spreading and short-term proliferation of osteoblasts, using human osteoblastic MG-63 cells. The films were characterized in terms of surface morphology and surface charge by atomic force microscopy and streaming potential measurements, respectively. Cell attachment was assessed after 3 and 24 h of cell culture. After 24 h of incubation, cell attachment was found to be dependent on the DA, lower DAs favouring cell adhesion. With time, cell spreading and cytoskeleton organization were only attained for DAs <= 13\%. Regarding cell proliferation, cells grown on films with the lowest DA (4\%) revealed a higher specific growth rate as compared to those grown on films with higher DAs. Films with a DA of 49\% failed to sustain cell proliferation. In addition, a longer lag-phase was observed on Ch, as compared to TCPS, in accordance to an observed delay of cell spreading. The present findings revealed that differences in the DA as small as 9\% may be critical in terms of the osteoblast response to two-dimensional Ch-based matrices. {\textcopyright} 2007 VSP.},
	keywords = {Acetylation, AFM, article, Atomic force microscopy, Biocompatible Materials, biofilm, Biofilms, biomaterial, bone cell, cartilage cell, cell adhesion, cell anchorage, Cell culture, cell growth, Cell Line, Tumor, cell proliferation, Cell Shape, cell spreading, cell survival, Chemical structure, chemistry, Chitin, chitosan, comparative study, controlled study, cytoskeleton, Degree of acetylation, drug effect, Growth kinetics, human, human cell, Humans, incubation time, metabolism, Microscopy, Atomic Force, osteoblast, Osteoblast-like cells, Osteoblasts, Pathology, priority journal, Surface charge, Surface Properties, surface property, Surface treatment, tumor cell line, Zeta potential},
	isbn = {09205063 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34347375992\&partnerID=40\&md5=437b9ed270e859425b76aa3655c611be},
	author = {Amaral, I.F. and Cordeiro, A.L. and Sampaio, P. and Barbosa, M.A.}
}
@article {2376,
	title = {Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix},
	journal = {BiomaterialsBiomaterials},
	volume = {28},
	year = {2007},
	note = {Cited By (since 1996): 39Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2007.04.028PubMed ID: 17532040Language of Original Document: EnglishCorrespondence Address: Evangelista, M.B.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mbe@ineb.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; alkaline phosphatase, 9001-78-9; arginylglycylaspartic acid, 99896-85-2; DNA, 9007-49-2; osteocalcin, 136461-80-8; Alginates; alginic acid, 9005-32-7; Alkaline Phosphatase, EC 3.1.3.1; arginyl-glycyl-aspartic acid, 99896-85-2; Glucuronic Acid, 576-37-4; Hexuronic Acids; Oligopeptides; Osteocalcin, 104982-03-8References: de Vos, P., de Haan, B., Wolters, G.H.J., VanSchilfgaarde, R., Factors influencing the adequacy of microencapsulation of rat pancreatic islets (1996) Transplantation, 62 (7), pp. 888-893;Brissova, M., Lacik, I., Powers, A.C., Anilkumar, A.V., Wang, T., Control and measurement of permeability for design of microcapsule cell delivery system (1998) J Biomed Mater Res, 39 (1), pp. 61-70; 
Strand, B.L., Gaserod, O., Kulseng, B., Espevik, T., Skjak-Braek, G., Alginate-polylysine-alginate microcapsules: effect of size reduction on capsule properties (2002) J Microencapsul, 19 (5), pp. 615-630; 
Chang, T.M.S., Artificial liver support based on artificial cells with emphasis on encapsulated hepatocytes (1992) Artif Organs, 16 (1), pp. 71-74; 
Uludag, H., Sefton, M.V., Microencapsulated human hepatoma (HepG2) cells-in-vitro growth and protein release (1993) J Biomed Mater Res, 27 (10), pp. 1213-1224; 
Canaple, L., Rehor, A., Hunkeler, D., Improving cell encapsulation through size control (2002) J Biomater Sci Polym Ed, 13 (7), pp. 783-796; 
Temenoff, J.S., Mikos, A.G., Injectable biodegradable materials for orthopedic tissue engineering (2000) Biomaterials, 21 (23), pp. 2405-2412; 
LeBaron, R.G., Athanasiou, K.A., Extracellular matrix cell adhesion peptides: functional applications in orthopedic materials (2000) Tissue Eng, 6 (2), pp. 85-103; 
Gumbiner, B.M., Cell adhesion: the molecular basis of tissue architecture and morphogenesis (1996) Cell, 84 (3), pp. 345-357; 
Majmudar, G., Bole, D., Goldstein, S.A., Bonadio, J., Bone cell-culture in a 3-dimensional polymer bead stabilizes the differentiated phenotype and provides evidence that osteoblastic cells synthesize type-III collagen and fibronectin (1991) J Bone Miner Res, 6 (8), pp. 869-881; 
Payne, R.G., Yaszemski, M.J., Yasko, A.W., Mikos, A.G., Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 1. Encapsulation of marrow stromal osteoblasts in surface crosslinked gelatin microparticles (2002) Biomaterials, 23 (22), pp. 4359-4371; 
Lee, K.Y., Alsberg, E., Mooney, D.J., Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering (2001) J Biomed Mater Res, 56 (2), pp. 228-233; 
Alsberg, E., Anderson, K.W., Albeiruti, A., Franceschi, R.T., Mooney, D.J., Cell-interactive alginate hydrogels for bone tissue engineering (2001) J Dental Res, 80 (11), pp. 2025-2029; 
Burdick, J.A., Anseth, K.S., Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering (2002) Biomaterials, 23 (22), pp. 4315-4323; 
Smidsrod, O., Skjak-Braek, G., Alginate as immobilization matrix for cells (1990) Trends Biotechnol, 8 (3), pp. 71-78; 
Boontheekul, T., Kong, H.J., Mooney, D.J., Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution (2005) Biomaterials, 26 (15), pp. 2455-2465; 
Kong, H.J., Alsberg, E., Kaigler, D., Lee, K.Y., Mooney, D.J., Controlling degradation of hydrogels via the size of cross-linked junctions (2004) Adv Mater, 16 (21), pp. 1917-1921; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., Alginate hydrogels as synthetic extracellular matrix materials (1999) Biomaterials, 20 (1), pp. 45-53; 
Bouhadir, K.H., Lee, K.Y., Alsberg, E., Damm, K.L., Anderson, K.W., Mooney, D.J., Degradation of partially oxidized alginate and its potential application for tissue engineering (2001) Biotechnol Prog, 17 (5), pp. 945-950; 
Kong, H.J., Smith, M.K., Mooney, D.J., Designing alginate hydrogels to maintain viability of immobilized cells (2003) Biomaterials, 24 (22), pp. 4023-4029; 
Bouhadir, K.H., Hausman, D.S., Mooney, D.J., Synthesis of cross-linked poly(aldehyde guluronate) hydrogels (1999) Polymer, 40 (12), pp. 3575-3584; 
Labarca, C., Paigen, K., Simple, rapid, and sensitive DNA assay procedure (1980) Anal Biochem, 102 (2), pp. 344-352; 
Caron, J.M., Kirschner, M.W., Autoregulation of tubulin synthesis (1986) Bioessays, 5 (5), pp. 211-216; 
Poole, C.A., Brookes, N.H., Clover, G.M., Keratocyte networks visualised in the living cornea using vital dyes (1993) J Cell Sci, 106, pp. 685-691; 
Li, R.H., Altreuter, D.H., Gentile, F.T., Transport characterization of hydrogel matrices for cell encapsulation (1996) Biotechnol Bioeng, 50 (4), pp. 365-373; 
Dulieu, C., Poncelet, D., Neufeld, R.J., Encapsulation and immobilization techniques (1999) Cell encapsulation technology and therapeutics, pp. 3-17. , K{\"u}htreiber W.M., Lanza R.P., and Chick W.L. (Eds), Birkh{\"a}user, Boston; 
Peirone, M., Ross, C.J.D., Hortelano, G., Brash, J.L., Chang, P.L., Encapsulation of various recombinant mammalian cell types in different alginate microcapsules (1998) J Biomed Mater Res, 42 (4), pp. 587-596; 
Kong, H.J., Kaigler, D., Kim, K., Mooney, D.J., Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution (2004) Biomacromolecules, 5 (5), pp. 1720-1727; 
Boninsegna, S., Dal Toso, R., Dal Monte, R., Alginate microspheres loaded with animal cells and coated by a siliceous layer (2003) J Sol-Gel Sci Technol, 26 (1-3), pp. 1151-1157; 
Rokstad, A.M., Donati, I., Borgogna, M., Oberholzer, J., Strand, B.L., Espevik, T., Cell-compatible covalently reinforced beads obtained from a chemoenzymatically engineered alginate (2006) Biomaterials, 27 (27), pp. 4726-4737; 
Lu, M.Z., Lan, H.L., Wang, F.F., Chang, S.J., Wang, Y.J., Cell encapsulation with alginate and alpha-phenoxycinnamylidene-acetylated poly(allylamine) (2000) Biotechnol Bioeng, 70 (5), pp. 479-483; 
Stein, G.S., Lian, J.B., Molecular mechanisms mediating proliferation differentiation interrelationships during progressive development of the osteoblast phenotype (1993) Endocrine Rev, 14 (4), pp. 424-442; 
Siebers, M.C., ter Brugge, P.J., Walboomers, X.F., Jansen, J.A., Integrins as linker proteins between osteoblasts and bone replacing materials. A critical review (2005) Biomaterials, 26 (2), pp. 137-146; 
Hynes, R.O., Integrins: bidirectional, allosteric signaling machines (2002) Cell, 110 (6), pp. 673-687; 
Giancotti, F.G., Ruoslahti, E., Transduction-integrin signaling (1999) Science, 285 (5430), pp. 1028-1032; 
Payne, R.G., McGonigle, J.S., Yaszemski, M.J., Yasko, A.W., Mikos, A.G., Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly(propylene fumarate) (2002) Biomaterials, 23 (22), pp. 4373-4380; 
Franceschi, R.T., Iyer, B.S., Relationship between collagen-synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells (1992) J Bone Miner Res, 7 (2), pp. 235-246; 
Franceschi, R.T., The developmental control of osteoblast-specific gene expression: role of specific transcription factors and the extracellular matrix environment (1999) Crit Rev Oral Biol Med, 10 (1), pp. 40-57; 
Gerstenfeld, L.C., Chipman, S.D., Kelly, C.M., Hodgens, K.J., Lee, D.D., Landis, W.J., Collagen expression, ultrastructural assembly, and mineralization in cultures of chicken-embryo osteoblasts (1988) J Cell Biol, 106 (3), pp. 979-989; 
Dudley, H.R., Spiro, D., Fine structure of bone cells (1961) J Biophys Biochem Cytol, 11 (3), pp. 627-649; 
Holtrop, M.E., The ultrastructure of bone (1975) Ann Clin Lab Sci, 5 (4), pp. 264-271; 
Rhodin, J.A.G., (1963) An atlas of ultrastructure, , W. B. Saunders Company, Philadelphia; 
Trojani, C., Weiss, P., Michiels, J.F., Vinatier, C., Guicheux, J., Daculsi, G., Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel (2005) Biomaterials, 26 (27), pp. 5509-5517; 
Kneser, U., Voogd, A., Ohnolz, J., Buettner, O., Stangenberg, L., Zhang, Y.H., Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute (2005) Cells Tissues Organs, 179 (4), pp. 158-169; 
Bancroft, J.D., Gamble, M., (2002) Theory and practice of histological techniques. 5th ed, , Elsevier Science, Edinburgh
},
	month = {2007///},
	pages = {3644 - 3655},
	abstract = {There is a need for new therapeutic strategies to treat bone defects caused by trauma, disease or tissue loss. Injectable systems for cell transplantation have the advantage of allowing the use of minimally invasive surgical procedures, and thus for less discomfort to patients. In the present study, it is hypothesized that Arg-Gly-Asp (RGD)-coupled in a binary (low and high molecular weight) injectable alginate composition is able to influence bone cell differentiation in a three-dimensional (3D) structure. Viability, metabolic activity, cytoskeleton organization, ultrastructure and differentiation (alkaline phosphatase (ALP), von Kossa, alizarin red stainings and osteocalcin quantification) of immobilized cells were assessed. Cells within RGD-modified alginate microspheres were able to establish more interactions with the synthetic extracellular matrix as visualized by confocal laser scanning microscope and transmission electron microscopy imaging, and presented a much higher level of differentiation (more intense ALP and mineralization stainings and higher levels of osteocalcin secretion) when compared to cells immobilized within unmodified alginate microspheres. These findings demonstrate that peptides covalently coupled to alginate were efficient in influencing cell behavior within this 3D system, and may provide adequate preparation of osteoblasts for cell transplantation. {\textcopyright} 2007 Elsevier Ltd. All rights reserved.},
	keywords = {3T3 Cells, Alginate, Alginates, alginic acid, alkaline phosphatase, animal cell, Animals, arginylglycylaspartic acid, article, Bone, bone cell, Bone Regeneration, calvaria, cell adhesion, cell differentiation, Cell encapsulation, Cell immobilization, cell metabolism, cell proliferation, cell survival, cell ultrastructure, cell viability, confocal laser microscopy, controlled study, cytology, cytoskeleton, DNA, DNA content, ECM (extracellular matrix), enzyme activity, extracellular matrix, Glucuronic Acid, Hexuronic Acids, hydrogel, immobilized cell, metabolism, Mice, Microscopy, Confocal, Microscopy, Electron, Transmission, microsphere, Microspheres, nonhuman, Oligopeptides, osteoblast, Osteoblasts, osteocalcin, Patient treatment, priority journal, rat, RGD peptide, structure analysis, three dimensional imaging, Tissue engineering, transmission electron microscopy, Transplantation (surgical)},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249870130\&partnerID=40\&md5=4476a4b1ee80320328c1bf37054037bb},
	author = {Evangelista, M.B. and Hsiong, S.X. and Fernandes, R. and Sampaio, P. and Kong, H.-J. and Barrias, C.C. and Salema, R. and Barbosa, M.A. and Mooney, D.J. and Granja, P.L.}
}
@article {2421,
	title = {Three-dimensional culture of human osteoblastic cells in chitosan sponges: The effect of the degree of acetylation},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {76},
	year = {2006},
	note = {Cited By (since 1996): 19Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30522PubMed ID: 16270345Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; chitosan, 9012-76-4; collagen, 9007-34-5; Alkaline Phosphatase, EC 3.1.3.1; Chitosan, 9012-76-4; Collagen, 9007-34-5References: Bruder, S.P., Caplan, A.I., Bone regeneration through cellular engineering (2000) Principles of Tissue Engineering, pp. 683-696. , Lanza RP, Langer R, Vacanti J, editors. San Diego: Academic Press;Anselme, K., Osteoblast adhesion on biomaterials (2000) Biomaterials, 21, pp. 667-681; 
Khor, E., (2001) Chitin: Fulfilling a Biomaterials Promise, , Oxford, UK: Elsevier Science Ltd; 
Vandevord, P.J., Hwt, M., DeSilva, S.P., Mayton, L., Wu, B., Wooley, P.H., Evaluation of the biocompatibility of a chitosan scaffold in mice (2002) J Biomed Mater Res, 59, pp. 585-590; 
Tomihata, K., Ikada, Y., In vitro and in vivo degradation of films of chitin and its deacetylated derivatives (1997) Biomaterials, 18, pp. 567-575; 
Rao, S.B., Sharma, C.P., Sterilization of chitosan: Implications (1995) J Biomater Appl, 10, pp. 136-143; 
Muzzarelli, R.A.A., Zucchini, C., Ilari, P., Pugnaloni, A., Belmonte, M.M., Biagini, G., Castaldini, C., Osteoconductive properties of methylpyrrolidinone chitosan in an animal-model (1993) Biomaterials, 14, pp. 925-929; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials (2002) J Control Release, 78, pp. 187-197; 
Zhao, F., Yin, Y.J., Lu, W.W., Leong, J.C., Zhang, W.J., Zhang, J.Y., Zhang, M.F., Yao, K.D., Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds (2002) Biomaterials, 23, pp. 3227-3234; 
Nettles, D.L., Elder, S.H., Gilbert, J.A., Potential use of chitosan as a cell scaffold material for cartilage tissue engineering (2002) Tissue Eng, 8, pp. 1009-1016; 
Zhang, Y., Zhang, M.Q., Calcium phosphate/chitosan composite scaffolds for controlled in vitro antibiotic drug release (2002) J Biomed Mater Res, 62, pp. 378-386; 
Prasitsilp, M., Jenwithisuk, R., Kongsuwan, K., Damrongchai, N., Watts, P., Cellular responses to chitosan in vitro: The importance of deacetylation (2000) J Mater Sci Mater Med, 11, pp. 773-778; 
Chatelet, C., Damour, O., Domard, A., Influence of the degree of acetylation on some biological properties of chitosan films (2001) Biomaterials, 22, pp. 261-268; 
Dhiman, H.K., Ray, A.R., Panda, A.K., Characterization and evaluation of chitosan matrix for in vitro growth of MCF-7 breast cancer cell lines (2004) Biomaterials, 25, pp. 5147-5154; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cells osteogenic differentiation and fibronectin adsorption on chitosan membranes: The effect of the degree of acetylation (2005) J Biomed Mater Res Part A, 75 A, pp. 387-397; 
Cukierman, E., Pankov, R., Stevens, D.R., Yamada, K.M., Taking cell-matrix adhesions to the third dimension (2001) Science, 294, pp. 1708-1712; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Mima, S., Miya, M., Iwamoto, R., Yoshikawa, S., Highly deacetylated chitosan and its properties (1983) J Appl Polym Sci, 28, pp. 1909-1917; 
Aiba, S., Studies on Chitosan: 1. Determination of the degree of N-acetylation of chitosan by ultraviolet spectrophotometry and gel-permeation chromatography (1986) Int J Biol Macromol, 8, pp. 173-176; 
Hirano, S., Ohe, Y., Ono, H., Selective N-acylation of chitosan (1976) Carbohydr Res, 47, pp. 315-320; 
Baxter, A., Dillon, M., Taylor, K.D.A., Roberts, G.A.F., Improved method for Ir determination of the degree of N-acetylation of chitosan (1992) Int J Biol Macromol, 14, pp. 166-169; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., Molecular parameters of chitosans depolymerized with the aid of papain (1996) Carbohydr Polym, 29, pp. 63-68; 
Zhang, R.Y., Ma, P.X., Poly(alpha-hydroxyl acids) hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology (1999) J Biomed Mater Res, 44, pp. 446-455; 
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J Immunol Methods, 65, pp. 55-63; 
Ashton, B.A., Abdullah, F., Cave, J., Williamson, M., Sykes, B.C., Couch, M., Poser, J.W., Characterization of cells with high alkaline-phosphatase activity derived from human-bone and marrow - Preliminary assessment of their osteogenicity (1985) Bone, 6, pp. 313-319; 
Kurita, K., Controlled functionalization of the polysaccharide chitin (2001) Prog Polym Sci, 26, pp. 1921-1971; 
Wang, W., Bo, S.Q., Li, S.Q., Qin, W., Determination of the Mark-Houwink equation for chitosans with different degrees of deacetylation (1991) Int J Biol Macromol, 13, pp. 281-285; 
Roberts, G.A.F., (1992) Chitin Chemistry, , Houndmills: The Macmillan Press Ltd; 
Ueda, H., Hong, L., Yamamoto, M., Shigeno, K., Inoue, M., Toba, T., Yoshitani, M., Shimizu, Y., Use of collagen sponge incorporating transforming growth factor-beta 1 to promote bone repair in skull defects in rabbits (2002) Biomaterials, 23, pp. 1003-1010; 
Hanthamrongwit, M., Grant, M.H., Wilkinson, R., Confocal laser-scanning microscopy (Clsm) for the study of collagen sponge microstructure (1994) J Biomed Mater Res, 28, pp. 213-216; 
Zinger, O., Anselme, K., Denzer, A., Habersetzer, P., Wieland, M., Jeanfils, J., Hardouin, P., Landolt, D., Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography (2004) Biomaterials, 25, pp. 2695-2711; 
Eggli, P.S., Muller, W., Schenk, R.K., Porous hydroxyapatite and tricalcium phosphate cylinders with 2 different pore-size ranges implanted in the cancellous bone of rabbits - A comparative histomorphometric and histologic-study of bony ingrowth and implant substitution (1988) Clin Orthop, 232, pp. 127-138; 
Madihally, S.V., Matthew, H.W.T., Porous chitosan scaffolds for tissue engineering (1999) Biomaterials, 20, pp. 1133-1142; 
Madihally, S.V., Frattaroli, T.L., Matthew, H.W.T., (1999) In Vivo Colonization of Chitosan Based Porous Scaffolds, p. 172. , Providence, RI: Transactions from the 25th Annual Meeting of the Society for Biomaterials, Providence, RI; 
Zhang, Y., Zhang, M.Q., Synthesis and characterization of macroporous chitosan/calcium phosphate composite scaffolds for tissue engineering (2001) J Biomed Mater Res, 55, pp. 304-312; 
Chung, T.W., Yang, J., Akaike, T., Cho, K.Y., Nah, J.W., Kim, S.I., Cho, C.S., Preparation of alginate/galactosylated chitosan scaffold for hepatocyte attachment (2002) Biomaterials, 23, pp. 2827-2834; 
Masi, L., Franchi, A., Santucci, M., Danielli, D., Arganini, L., Giannone, V., Formigli, L., Beghe, F., Adhesion, growth, and matrix production by osteoblasts on collagen substrata (1992) Calcif Tissue Int, 51, pp. 202-212; 
Wiesmann, H.P., Nazer, N., Klatt, C., Szuwart, T., Meyer, U., Bone tissue engineering by primary osteoblast-like cells in a monolayer system and 3-dimensional collagen gel (2003) J Oral Maxillofac Surg, 61, pp. 1455-1462; 
Henriksen, I., Green, K.L., Smart, J.D., Smistad, G., Karlsen, J., Bioadhesion of hydrated chitosans: An in vitro and in vivo study (1996) Int J Pharmaceut, 145, pp. 231-240; 
Lebaron, R.G., Athanasiou, K.A., Extracellular matrix cell adhesion peptides: Functional applications in orthopedic materials (2000) Tissue Eng, 6, pp. 85-103; 
Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., Adhesive protein interactions with chitosan: Consequences for valve endothelial cell growth on tissue-engineering materials (2003) J Biomed Mater Res Part A, 67 A, pp. 538-547; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res Part A, 66 A, pp. 247-259; 
Webb, K., Hlady, V., Tresco, P.A., Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces (2000) J Biomed Mater Res, 49, pp. 362-368; 
Anselme, K., Bigerelle, M., Noel, B., Dufresne, E., Judas, D., Lost, A., Hardouin, P., Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses (2000) J Biomed Mater Res, 49, pp. 155-166; 
Domard, A., Chitosan interactions (1997) Advances in Chitin Science, pp. 410-420. , Domard A, Roberts GAF, Varum KM, editors. Lyon, France: Jacques Andr{\'e} Publisher;
},
	month = {2006///},
	pages = {335 - 346},
	abstract = {In this investigation, the effect of the degree of acetylation (DA) of chitosan on the behavior of human osteoblastic MG-63 cells cultured in three-dimensional chitosan matrices was assessed. Chitosan sponges with DAs in the range of 4 to 49\% were prepared and characterized in terms of microstructure, porosity, and pore size. Collagen sponges were used as 3D control. Cell proliferation was determined using the MTT assay while the retention of the osteoblastic phenotype was monitored by assaying alkaline phosphatase activity. Cell morphology, cytoskeletal organization, and viability were assessed using different microscopy techniques. Chitosan sponges showed a similar microstructure regardless the DA, except for the highest DA used, where a more heterogeneous pore distribution was observed. In terms of cell proliferation, alkaline phosphatase activity and cell viability, cells cultured in chitosan scaffolds performed as well as in the 3D control regardless the DA, except for the highest DA used, where an inhibitory effect on cell proliferation was found. However, while in sponges with DAs <= 13\% cells attached and spread displaying long cell filopodia and numerous cell-to-cell contacts, in sponges with higher DAs cells tended to remain spherical and grow into spheroid-like cellular aggregates. In the present study, the DA played a key role in determining the affinity of osteoblastic cells towards the substrates, possibly by influencing the nature of the initial adsorbed protein layer. {\textcopyright} 2005 Wiley Periodicals, Inc.},
	keywords = {Acetylation, Aggregates, alkaline phosphatase, article, Bone Regeneration, bone tissue, Bone tissue engineering, Cell culture, Cell Culture Techniques, Cell filopodia, cell growth, cell proliferation, Cell Shape, cell structure, cell survival, cell viability, chitosan, Collagen, confocal microscopy, controlled study, Cytoskeletal organization, cytoskeleton, Degree of acetylation (DA), enzyme activity, gel permeation chromatography, human, human cell, Humans, infrared spectroscopy, Microstructure, morphology, osteoblast, Osteoblastic cells, Osteoblasts, Phosphatase activity, porosity, Proteins, Scaffold, Scaffolds, Scanning electron microscopy, Substrates, Tissue engineering},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645101773\&partnerID=40\&md5=5adc6336963ec1c8a83d8ec89943159d},
	author = {Amaral, I.F. and Sampaio, P. and Barbosa, M.A.}
}
@article {2444,
	title = {Rat bone marrow stromal cell osteogenic differentiation and fibronectin adsorption on chitosan membranes: The effect of the degree of acetylation},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {75},
	year = {2005},
	note = {Cited By (since 1996): 25Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30436PubMed ID: 16092111Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; fibronectin, 86088-83-7; polystyrene, 9003-53-6; Chitosan, 9012-76-4; Coated Materials, Biocompatible; FibronectinsReferences: VandeVord, P.J., Matthew, H.W.T., DeSilva, S.P., Mayton, L., Wu, B., Wooley, P.H., Evaluation of the biocompatibility of a chitosan scaffold in mice (2002) J Biomed Mater Res, 59 (3), pp. 585-590;Muzzarelli, R.A.A., Human enzymatic activities related to the therapeutic administration of chitin derivatives (1997) Cell Mol Life Sci, (53), pp. 131-140; 
Peluso, G., Petillo, O., Ranieri, M., Santin, M., Ambrosio, L., Calabro, D., Avallone, B., Balsamo, G., Chitosan-mediated stimulation of macrophage function (1994) Biomaterials, 15 (15), pp. 1215-1220; 
Muzzarelli, R.A.A., Mattiolibelmonte, M., Tietz, C., Biagini, R., Ferioli, G., Brunelli, M.A., Fini, M., Biagini, G., Stimulatory effect on bone-formation exerted by a modified chitosan (1994) Biomaterials, 15 (13), pp. 1075-1081; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials (2002) J Controlled Release, 78 (1-3), pp. 187-197; 
Chupa, J.M., Foster, A.M., Sumner, S.R., Madihally, S.V., Matthew, H.W.T., Vascular cell responses to polysaccharide materials: In vitro and in vivo evaluations (2000) Biomaterials, 21 (22), pp. 2315-2322; 
Lahiji, A., Sohrabi, A., Hungerford, D.S., Frondoza, C.G., Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes (2000) J Biomed Mater Res, 51 (4), pp. 586-595; 
Klokkevold, P.R., Vandemark, L., Kenney, E.B., Bernard, G.W., Osteogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro (1996) J Periodontol, 67 (11), pp. 1170-1175; 
Tomihata, K., Ikada, Y., In vitro and in vivo degradation of films of chitin and its deacetylated derivatives (1997) Biomaterials, 18 (7), pp. 567-575; 
Chatelet, C., Damour, O., Domard, A., Influence of the degree of acetylation on some biological properties of chitosan films (2001) Biomaterials, 22 (3), pp. 261-268; 
Dhiman, H.K., Ray, A.R., Panda, A.K., Characterization and evaluation of chitosan matrix for in vitro growth of MCF-7 breast cancer cell lines (2004) Biomaterials, 25 (21), pp. 5147-5154; 
Prasitsilp, M., Jenwithisuk, R., Kongsuwan, K., Damrongchai, N., Watts, P., Cellular responses to chitosan in vitro: The importance of deacetylation (2000) J Mater Sci Mater Med, 11 (12), pp. 773-778; 
Rainaldi, G., Filippini, P., Ferrante, A., Indovina, P.L., Santini, M.T., Fibronectin facilitates adhesion of K562 leukemic cells normally growing in suspension to cationic surfaces (2001) J Biomed Mater Res, 55 (1), pp. 104-113; 
Faucheux, N., Schweiss, R., Lutzow, K., Werner, C., Groth, T., Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies (2004) Biomaterials, 25 (14), pp. 2721-2730; 
Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., Adhesive protein interactions with chitosan: Consequences for valve endothelial cell growth on tissue-engineering materials (2003) J Biomed Mater Res, 67 A (2), pp. 538-547; 
Bumgardner, J.D., Wiser, R., Elder, S.H., Jouett, R., Yang, Y., Ong, J.L., Contact angle, protein adsorption and osteoblast precursor cell attachment to chitosan coatings bonded to titanium (2003) J Biomater Sci Polym Ed, 14 (12), pp. 1401-1409; 
Gong, H.P., Zhong, Y.H., Li, J.C., Gong, Y.D., Zhao, N.M., Zhang, X.F., Studies on nerve cell affinity of chitosan-derived materials (2000) J Biomed Mater Res, 52 (2), pp. 285-295; 
Benesch, J., Tengvall, P., Blood protein adsorption onto chitosan (2002) Biomaterials, 23 (12), pp. 2561-2568; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42 (8), pp. 3569-3580; 
Mima, S., Miya, M., Iwamoto, R., Yoshikawa, S., Highly deacetylated chitosan and its properties (1983) J Appl Polym Sci, 28 (6), pp. 1909-1917; 
Aiba, S., Studies on chitosan: 1. Determination of the degree of N-acetylation of chitosan by ultraviolet spectrophotometry and gel-permeation chromatography (1986) Int J Biol Macromol, 8 (3), pp. 173-176; 
Baxter, A., Dillon, M., Taylor, K.D.A., Roberts, G.A.F., Improved method for i.r. determination of the degree of N-acetylation of chitosan (1992) Int J Biol Macromol, 14 (3), pp. 166-169; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., Molecular parameters of chitosans depolymerized with the aid of papain (1996) Carbohydr Polym, 29 (1), pp. 63-68; 
Grundke, K., Bogumil, T., Werner, C., Janke, A., Poschel, K., Jacobasch, H.J., Liquid-fluid contact angle measurements on hydrophilic cellulosic materials (1996) Colloids Surf A, 116 (1-2), pp. 79-91; 
Slack, M., Properties of biological fluids (2004) Biomaterials Science: An Introduction to Materials in Medicine, pp. 813-817. , Ratner BD, Hoffman AS, Schoen FJ, Lemons JE, editors. Amsterdam: Elsevier; 
Du, Y.J., Cornelius, R.M., Brash, J.L., Measurement of protein adsorption to gold surface by radioiodination methods: Suppression of free iodide Sorption (2000) Colloids Surf B, 17 (1), pp. 59-67; 
(1993) Iodine-125, a Guide to Radioiodination Techniques, p. 64. , Little Chalfont, Buckinghamshire, UK: Amersham International; 
Sousa, S.R., Moradas-Ferreira, P., Saramago, B., Melo, L.V., Barbosa, M.A., Human serum albumin adsorption on TiO2 from single protein solutions and from plasma (2004) Langmuir, 20 (22), pp. 9745-9754; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone-formation in vitro by stromal cells obtained from bone-marrow of young-adult rats (1988) Cell Tissue Res, 254 (2), pp. 317-330; 
Ashton, B.A., Abdullah, F., Cave, J., Williamson, M., Sykes, B.C., Couch, M., Poser, J.W., Characterization of cells with high alkaline-phosphatase activity derived from human-bone and marrow: Preliminary assessment of their osteogenicity (1985) Bone, 6 (5), pp. 313-319; 
Huang, R.Y.M., Moon, G.Y., Pal, R., Chitosan/anionic surfactant complex membranes for the pervaporation separation of methanol/MTBE and characterization of the polymer/surfactant system (2001) J Membr Sei, 184 (1), pp. 1-15; 
Domke, J., Dannohl, S., Parak, W.J., Muller, O., Aicher, W.K., Radmacher, M., Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy (2000) Colloids Surf B, 19 (4), pp. 367-379; 
Anselme, K., Osteoblast adhesion on biomaterials (2000) Biomaterials, 21 (7), pp. 667-681; 
Domard, A., Chitosan interactions (1997) Advances in Chitin Science, pp. 410-420. , Domard A, Roberts GAF, Varum KM, editors. Lyon, France: Jacques Andr{\'e} Publisher; 
Smidsrod, O., Ottoy, M.H., Anthonsen, M.W., Varum, K.M., Solution properties of chitosan (1997) Advances in Chitin Science, pp. 402-409. , Domard A, Roberts GAF, Varum KM, editors. Lyon, France: Jacques Andr{\'e} Publisher; 
Fee, M., Errington, N., Jumel, K., Ilium, L., Smith, A., Harding, S.E., Correlation of SEC/MALLS with ultracentrifuge and viscometric data for chitosans (2003) Eur Biophys J, 32, pp. 457-464; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res, 66 A (2), pp. 247-259; 
Andrade, J.D., Smith, L.M., Gregonis, D.E., The contact angle and interface energetics (1997) Physical Chemistry of Surfaces, pp. 249-292. , Adamson AW, Gast AP, editors. New York: John Wiley \& Sons;; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., Attachment of cultured human bone cells to novel polymers (1999) J Biomed Mater Res, 44 (1), pp. 1-11; 
Lebaron, R.G., Athanasiou, K.A., Extracellular matrix cell adhesion peptides: Functional applications in orthopedic materials (2000) Tissue Eng, 6 (2), pp. 85-103; 
Massia, S.P., Hubbell, J.A., An RGD spacing of 440 run is sufficient for integrin alpha-V-beta-3- mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation (1991) J Cell Biol, 114 (5), pp. 1089-1100; 
Verrier, S., Fallu, S., Bareille, R., Jonczyk, A., Meyer, J., Dard, M., Amedee, J., Function of linear and cyclic RGD-containing peptides in osteoprogenitor cells adhesion process (2002) Biomaterials, 23 (2), pp. 585-596; 
Gronthos, S., Sirnmons, P.J., Graves, S.E., Robey, P.G., Integrin-mediated interactions between human bone marrow stromal precursor cells and the extracellular matrix (2001) Bone, 28 (2), pp. 174-181; 
Webb, K., Hlady, V., Tresco, P.A., Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces (2000) J Biomed Mater Res, 49 (3), pp. 362-368; 
Stein, G.S., Lian, J.B., Stein, J.L., Wijnen, A.J., Frenkel, B., Montecino, M., Mechanisms regulating osteoblast proliferation and differentiation (1996) Principles of Bone Biology, pp. 69-86. , Bilezikian JP, Raisz LG, Rodan GA, editors. San Diego: Academic Press; 
Aubin, J.E., Liu, F., The osteoblast lineage (1996) Principles of Bone Biology, pp. 51-67. , Bilezikian JP, Raisz LG, Rodan GA, editors. San Diego: Academic Press; 
Moursi, A.M., Globus, R.K., Damsky, C.H., Interactions between integrin receptors and fibronectin are required for calvarial osteoblast differentiation in vitro (1997) J Cell Sci, 110, pp. 2187-2196; 
Stephansson, S.N., Byers, B.A., Garcia, A.J., Enhanced expression of the osteoblastic phenotype on substrates that modulate fibronectin conformation and integrin receptor binding (2002) Biomaterials, 23 (12), pp. 2527-2534
},
	month = {2005///},
	pages = {387 - 397},
	abstract = {Cell adhesion, migration, and proliferation of a few anchorage-dependent cells cultured on chitosan (Ch) matrices are influenced by the degree of N-acetylation (DA) of Ch. In the present work, we examined the influence of the DA on the attachment, spreading, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (rBM-SCs). Ch membranes were characterized in terms of surface morphology, roughness, and wettability, and in terms of adsorption of an adhesive protein, fibronectin (Fn). Chs with DAs in the range of 4 to 49\% were used. Among the Ch samples, the DA of 4\% led to the highest Fn surface concentration, both from single protein solution and from diluted serum. Furthermore, the levels of Fn adsorbed from serum found for this DA were threefold higher than for the tissue culture polystyrene control, indicating that in the presence of competitive proteins Ch is more specific toward Fn adsorption than tissue culture polystyrene. rBMSCs cultured on Ch carrying a DA of 4\% were able to spread, proliferate, and differentiate, reaching a higher level of osteogenic differentiation than on the control, despite the lower cell attachment observed for all Ch samples. Because the Ch sample with a DA of 4\% showed the highest Fn adsorption from serum, we suggest that cell adhesion, spreading, and osteogenic differentiation of rBMSCs on Ch may be mediated by the adsorbed layer of Fn. {\textcopyright} 2005 Wiley Periodicals, Inc.},
	keywords = {Acetylation, Adhesion, Adsorption, animal cell, Animals, article, artificial membrane, binding affinity, binding competition, Bone, bone development, bone marrow cell, Bone Marrow Cells, Bone marrow stomal cells, Bone marrow stromal cells, cell adhesion, cell anchorage, Cell culture, cell differentiation, Cell membranes, cell migration, cell proliferation, Cell Shape, cell spreading, chitosan, Coated Materials, Biocompatible, controlled study, cytoskeleton, Degree of acetylation, Fibronectin, Fibronectins, male, materials testing, morphology, nonhuman, polystyrene, Proteins, rat, Rats, Rats, Wistar, roughness, stroma cell, Stromal Cells, Surface Properties, surface property, Tissue culture, wettability},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-26844551535\&partnerID=40\&md5=fa91e3e29d946053ae7bb231b6a2640e},
	author = {Amaral, I.F. and Lamghari, M. and Sousa, S.R. and Sampaio, P. and Barbosa, M.A.}
}
