



@article {3319,
	title = {Modified-chitosan/siRNA nanoparticles downregulate cellular CDX2 expression and cross the gastric mucus barrier},
	journal = {PLoS ONE},
	volume = {9},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Development of effective non-viral vectors is of crucial importance in the implementation of RNA interference in clinical routine. The localized delivery of siRNAs to the gastrointestinal mucosa is highly desired but faces specific problems such as the stability in gastric acidity conditions and the presence of the mucus barrier. CDX2 is a transcription factor critical for intestinal differentiation being involved in the initiation and maintenance of gastrointestinal diseases. Specifically, it is the trigger of gastric intestinal metaplasia which is a precursor lesion of gastric cancer. Its expression is also altered in colorectal cancer, where it may constitute a lineage-survival oncogene. Our main objective was to develop a nanoparticle-delivery system of siRNA targeting CDX2 using modified chitosan as a vector. CDX2 expression was assessed in gastric carcinoma cell lines and nanoparticles behaviour in gastrointestinal mucus was tested in mouse explants. We show that imidazolemodified chitosan and trimethylchitosan/siRNA nanoparticles are able to downregulate CDX2 expression and overpass the gastric mucus layer but not colonic mucus. This system might constitute a potential therapeutic approach to treat CDX2-dependent gastric lesions. {\textcopyright} 2014 Sadio et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903390267\&partnerID=40\&md5=61e637790e0edeb37d05789120975646},
	author = {Sadio, A. and Gustafsson, J.K. and Pereira, B. and Gomes, C.P. and Hansson, G.C. and David, L. and P{\^e}go, A.P. and Almeida, R.}
}
@article {3316,
	title = {Poly(trimethylene carbonate-co-ε-caprolactone) promotes axonal growth},
	journal = {PLoS ONE},
	volume = {9},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Mammalian central nervous system (CNS) neurons do not regenerate after injury due to the inhibitory environment formed by the glial scar, largely constituted by myelin debris. The use of biomaterials to bridge the lesion area and the creation of an environment favoring axonal regeneration is an appealing approach, currently under investigation. This work aimed at assessing the suitability of three candidate polymers - poly(ε-caprolactone), poly(trimethylene carbonate-co-ε-caprolactone) (P(TMC-CL)) (11:89 mol\%) and poly(trimethylene carbonate) - with the final goal of using these materials in the development of conduits to promote spinal cord regeneration. Poly(L-lysine) (PLL) coated polymeric films were tested for neuronal cell adhesion and neurite outgrowth. At similar PLL film area coverage conditions, neuronal polarization and axonal elongation was significantly higher on P(TMC-CL) films. Furthermore, cortical neurons cultured on P(TMC-CL) were able to extend neurites even when seeded onto myelin. This effect was found to be mediated by the glycogen synthase kinase 3β (GSK3β) signaling pathway with impact on the collapsin response mediator protein 4 (CRMP4), suggesting that besides surface topography, nanomechanical properties were implicated in this process. The obtained results indicate P(TMC-CL) as a promising material for CNS regenerative applications as it promotes axonal growth, overcoming myelin inhibition. {\textcopyright} 2014 Rocha et al.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84896121626\&partnerID=40\&md5=8f29642bf67ec86cf774379d7d92e7b0},
	author = {Rocha, D.N. and Brites, P. and Fonseca, C. and P{\^e}go, A.P.}
}
@article {3267,
	title = {Rescue of wild-type E-cadherin expression from nonsense-mutated cancer cells by a suppressor-tRNA},
	journal = {European Journal of Human Genetics},
	volume = {22},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1085 - 1092},
	abstract = {Hereditary diffuse gastric cancer (HDGC) syndrome, although rare, is highly penetrant at an early age, and is severe and incurable because of ineffective screening tools and therapy. Approximately 45\% of HDGC families carry germline CDH1/E-cadherin alterations, 20\% of which are nonsense leading to premature protein truncation. Prophylactic gastrectomy is the only recommended approach for all asymptomatic CDH1 mutation carriers. Suppressor-tRNAs can replace premature stop codons (PTCs) with a cognate amino acid, inducing readthrough and generating full-length proteins. The use of suppressor-tRNAs in HDGC patients could therefore constitute a less invasive therapeutic option for nonsense mutation carriers, delaying the development of gastric cancer. Our analysis revealed that 23/108 (21.3\%) of E-cadherin-mutant families carried nonsense mutations that could be potentially corrected by eight suppressor-tRNAs, and arginine was the most frequently affected amino acid. Using site-directed mutagenesis, we developed an arginine suppressor-tRNA vector to correct one HDGC nonsense mutation. E-cadherin-deficient cell lines were transfected with plasmids carrying simultaneously the suppressor-tRNA and wild-type or mutant CDH1 mini-genes. RT-PCR, western blot, immunofluorescence, flow cytometry and proximity ligation assay (PLA) were used to establish the model, and monitor mRNA and protein expression and function recovery from CDH1 vectors. Cells expressing a CDH1 mini-gene, carrying a nonsense mutation and the suppressor-tRNA, recovered full-length E-cadherin expression and its correct localization and incorporation into the adhesion complex. This is the first demonstration of functional recovery of a mutated causative gene in hereditary cancer by cognate amino acid replacement with suppressor-tRNAs. Of the HDGC families, 21.3\% are candidates for correction with suppressor-tRNAs to potentially delay cancer onset. {\textcopyright} 2014 Macmillan Publishers Limited. All rights reserved.},
	keywords = {hereditary diffuse gastric cancer (HDGC), nonsense suppression, Premature termination codon (PTC), Suppressor-tRNA},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84906269658\&partnerID=40\&md5=2feed844660a13385857a7e5d4470a2b},
	author = {Bordeira-Carri{\c c}o, R. and Ferreira, D. and Mateus, D.D. and Pinheiro, H. and P{\^e}go, A.P. and Santos, M.A.S. and Oliveira, C.}
}
@article {2880,
	title = {Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7-10)},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {5643 - 5652},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84873199566\&partnerID=40\&md5=f548695261ada2fc57ece26bff1f45d0},
	author = {Amaral, I.F. and Neiva, I. and Ferreira da Silva, F. and Sousa, S.R. and Piloto, A.M. and Lopes, C.D.F. and Barbosa, M.A. and Kirkpatrick, C.J. and P{\^e}go, A.P.}
}
@article {2866,
	title = {Hyaluronic acid / chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes},
	journal = {Journal of Controlled Release},
	volume = {168},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {289 - 297},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877033262\&partnerID=40\&md5=d58ff56593b3a791351102271ca61440},
	author = {Hartmann, H. and Hossfeld, S. and Schlosshauer, B. and Mittnacht, U. and P{\^e}go, A.P. and Dauner, M. and Doser, M. and Stoll, D. and Krastev, R.}
}
@article {2881,
	title = {Taking tissue adhesives to the future: From traditional synthetic to new biomimetic approaches},
	journal = {Biomaterials Science},
	volume = {1},
	year = {2013},
	note = {Cited By (since 1996):3Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {239 - 253},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877753912\&partnerID=40\&md5=772c15e73287e3754da848ada5a0e296},
	author = {Br{\'e}, L.P. and Zheng, Y. and P{\^e}go, A.P. and Wang, W.}
}
@article {2182,
	title = {Cancer syndromes and therapy by stop-codon readthrough},
	journal = {Trends in Molecular MedicineTrends Mol. Med.},
	volume = {18},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: TMMRCdoi: 10.1016/j.molmed.2012.09.004Language of Original Document: EnglishCorrespondence Address: Oliveira, C.; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), 4200-465 Porto, Portugal; email: carlaol@ipatimup.ptReferences: Linde, L., Kerem, B., Introducing sense into nonsense in treatments of human genetic diseases (2008) Trends Genet., 24, pp. 552-563;Isken, O., Maquat, L.E., The multiple lives of NMD factors: balancing roles in gene and genome regulation (2008) Nat. Rev. Genet., 9, pp. 699-712; 
Lee, H.L., Dougherty, J.P., Pharmaceutical therapies to recode nonsense mutations in inherited diseases (2012) Pharmacol. Ther., 136, pp. 227-266; 
Stenson, P.D., The Human Gene Mutation Database: providing a comprehensive central mutation database for molecular diagnostics and personalized genomics (2009) Hum. Genomics, 4, pp. 69-72; 
Sloane, P.A., Rowe, S.M., Cystic fibrosis transmembrane conductance regulator protein repair as a therapeutic strategy in cystic fibrosis (2010) Curr. Opin. Pulm. Med., 16, pp. 591-597; 
Kerr, T.P., Long mutant dystrophins and variable phenotypes: evasion of nonsense-mediated decay? (2001) Hum. Genet., 109, pp. 402-407; 
Berger, A.H., A continuum model for tumour suppression (2011) Nature, 476, pp. 163-169; 
Oliveira, C., Quantification of epigenetic and genetic 2nd hits in CDH1 during hereditary diffuse gastric cancer syndrome progression (2009) Gastroenterology, 136, pp. 2137-2148; 
Schneikert, J., Behrens, J., The canonical Wnt signalling pathway and its APC partner in colon cancer development (2007) Gut, 56, pp. 417-425; 
van der Groep, P., Pathology of hereditary breast cancer (2011) Cell. Oncol. (Dordr.), 34, pp. 71-88; 
Oliveira, C., Germline CDH1 deletions in hereditary diffuse gastric cancer families (2009) Hum. Mol. Genet., 18, pp. 1545-1555; 
Fitzgerald, R.C., Hereditary diffuse gastric cancer: updated consensus guidelines for clinical management and directions for future research (2010) J. Med. Genet., 47, pp. 436-444; 
Fearon, E.R., Molecular genetics of colorectal cancer (2011) Annu. Rev. Pathol., 6, pp. 479-507; 
Beroud, C., Soussi, T., APC gene: database of germline and somatic mutations in human tumors and cell lines (1996) Nucleic Acids Res., 24, pp. 121-124; 
Minde, D.P., Messing up disorder: how do missense mutations in the tumor suppressor protein APC lead to cancer? (2011) Mol. Cancer, 10, p. 101; 
Half, E., Familial adenomatous polyposis (2009) Orphanet J. Rare Dis., 4, p. 22; 
Knudsen, A.L., Attenuated familial adenomatous polyposis: results from an international collaborative study (2010) Colorectal Dis., 12, pp. e243-e249; 
Papp, J., Germline MLH1 and MSH2 mutational spectrum including frequent large genomic aberrations in Hungarian hereditary non-polyposis colorectal cancer families: implications for genetic testing (2007) World J. Gastroenterol., 13, pp. 2727-2732; 
Marsh, D.J., Mutation spectrum and genotype-phenotype analyses in Cowden disease and Bannayan-Zonana syndrome, two hamartoma syndromes with germline PTEN mutation (1998) Hum. Mol. Genet., 7, pp. 507-515; 
Beggs, A.D., Peutz-Jeghers syndrome: a systematic review and recommendations for management (2010) Gut, 59, pp. 975-986; 
Amos, C.I., Genotype-phenotype correlations in Peutz-Jeghers syndrome (2004) J. Med. Genet., 41, pp. 327-333; 
Ognjanovic, S., Sarcomas in TP53 germline mutation carriers: a review of the IARC TP53 database (2012) Cancer, 118, pp. 1387-1396; 
Miki, Y., A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 (1994) Science, 266, pp. 66-71; 
Goldberg, J.I., Borgen, P.I., Breast cancer susceptibility testing: past, present and future (2006) Expert Rev. Anticancer Ther., 6, pp. 1205-1214; 
Hakansson, S., Moderate frequency of BRCA1 and BRCA2 germ-line mutations in Scandinavian familial breast cancer (1997) Am. J. Hum. Genet., 60, pp. 1068-1078; 
Serova, O., A high incidence of BRCA1 mutations in 20 breast-ovarian cancer families (1996) Am. J. Hum. Genet., 58, pp. 42-51; 
Szabo, C.I., Understanding germ-line mutations in BRCA1 (2004) Cancer Biol. Ther., 3, pp. 515-520; 
Karam, R., The NMD mRNA surveillance pathway downregulates aberrant E-cadherin transcripts in gastric cancer cells and in CDH1 mutation carriers (2008) Oncogene, 27, pp. 4255-4260; 
Neu-Yilik, G., Mechanism of escape from nonsense-mediated mRNA decay of human beta-globin transcripts with nonsense mutations in the first exon (2011) RNA, 17, pp. 843-854; 
Slayton, R.L., Nonsense mutations in the COL1A1 gene preferentially reduce nuclear levels of mRNA but not hnRNA in osteogenesis imperfecta type I cell strains (2000) Matrix Biol., 19, pp. 1-9; 
Niyibizi, C., Gene therapy approaches for osteogenesis imperfecta (2004) Gene Ther., 11, pp. 408-416; 
Keeling, K.M., Bedwell, D.M., Suppression of nonsense mutations as a therapeutic approach to treat genetic diseases (2011) Wiley Interdiscip. Rev. RNA, 2, pp. 837-852; 
Perrin-Vidoz, L., The nonsense-mediated mRNA decay pathway triggers degradation of most BRCA1 mRNAs bearing premature termination codons (2002) Hum. Mol. Genet., 11, pp. 2805-2814; 
Fearnhead, N.S., The ABC of APC (2001) Hum. Mol. Genet., 10, pp. 721-733; 
Castellsague, E., Allele-specific expression of APC in adenomatous polyposis families (2010) Gastroenterology, 139, pp. 439-447. , 439-447, 447.e1; 
Kellermayer, R., Aminoglycosides as potential pharmacogenetic agents in the treatment of Hailey-Hailey disease (2006) J. Invest. Dermatol., 126, pp. 229-231; 
Allamand, V., Drug-induced readthrough of premature stop codons leads to the stabilization of laminin α2 chain mRNA in CMD myotubes (2008) J. Gene Med., 10, pp. 217-224; 
Welch, E.M., PTC124 targets genetic disorders caused by nonsense mutations (2007) Nature, 447, pp. 87-91; 
Carnes, J., Suppression of eukaryotic translation termination by selected RNAs (2000) RNA, 6, pp. 1468-1479; 
Carnes, J., Stop codon suppression via inhibition of eRF1 expression (2003) RNA, 9, pp. 648-653; 
Capone, J.P., Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene (1985) EMBO J., 4, pp. 213-221; 
Nakamura, A., Takeda, S., Mammalian models of Duchenne Muscular Dystrophy: pathological characteristics and therapeutic applications (2011) J. Biomed. Biotechnol., 2011, p. 184393; 
Linde, L., Nonsense-mediated mRNA decay affects nonsense transcript levels and governs response of cystic fibrosis patients to gentamicin (2007) J. Clin. Invest., 117, pp. 683-692; 
Linde, L., The efficiency of nonsense-mediated mRNA decay is an inherent character and varies among different cells (2007) Eur. J. Hum. Genet., 15, pp. 1156-1162; 
Keeling, K.M., Leaky termination at premature stop codons antagonizes nonsense-mediated mRNA decay in S. cerevisiae (2004) RNA, 10, pp. 691-703; 
Sako, Y., A novel therapeutic approach for genetic diseases by introduction of suppressor tRNA (2006) Nucleic Acids Symp. Ser. (Oxf.), 50, pp. 239-240; 
Hainrichson, M., Designer aminoglycosides: the race to develop improved antibiotics and compounds for the treatment of human genetic diseases (2008) Org. Biomol. Chem., 6, pp. 227-239; 
Kellermayer, R., Translational readthrough induction of pathogenic nonsense mutations (2006) Eur. J. Med. Genet., 49, pp. 445-450; 
Heier, C.R., DiDonato, C.J., Translational readthrough by the aminoglycoside geneticin (G418) modulates SMN stability in vitro and improves motor function in SMA mice in vivo (2009) Hum. Mol. Genet., 18, pp. 1310-1322; 
Arakawa, M., Negamycin restores dystrophin expression in skeletal and cardiac muscles of mdx mice (2003) J. Biochem., 134, pp. 751-758; 
Nudelman, I., Repairing faulty genes by aminoglycosides: development of new derivatives of geneticin (G418) with enhanced suppression of diseases-causing nonsense mutations (2010) Bioorg. Med. Chem., 18, pp. 3735-3746; 
Nudelman, I., Development of novel aminoglycoside (NB54) with reduced toxicity and enhanced suppression of disease-causing premature stop mutations (2009) J. Med. Chem., 52, pp. 2836-2845; 
Du, M., PTC124 is an orally bioavailable compound that promotes suppression of the human CFTR-G542X nonsense allele in a CF mouse model (2008) Proc. Natl. Acad. Sci. U.S.A., 105, pp. 2064-2069; 
Hirawat, S., Safety, tolerability, and pharmacokinetics of PTC124, a nonaminoglycoside nonsense mutation suppressor, following single- and multiple-dose administration to healthy male and female adult volunteers (2007) J. Clin. Pharmacol., 47, pp. 430-444; 
Teng, S., Readthrough of nonsense mutation W822X in the SCN5A gene can effectively restore expression of cardiac Na+ channels (2009) Cardiovasc. Res., 83, pp. 473-480; 
Diop, D., Aminoglycosides and other factors promoting stop codon readthrough in human cells (2007) C. R. Biol., 330, pp. 71-79; 
Buvoli, M., Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes (2000) Mol. Cell. Biol., 20, pp. 3116-3124; 
von der Haar, T., Tuite, M.F., Regulated translational bypass of stop codons in yeast (2007) Trends Microbiol., 15, pp. 78-86; 
Rodriguez, E.A., In vivo incorporation of multiple unnatural amino acids through nonsense and frameshift suppression (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 8650-8655; 
Panchal, R.G., Partial functional correction of xeroderma pigmentosum group A cells by suppressor tRNA (1999) Hum. Gene Ther., 10, pp. 2209-2219; 
Bhuvanagiri, M., NMD: RNA biology meets human genetic medicine (2010) Biochem. J., 430, pp. 365-377; 
Gubbens, J., In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs (2010) RNA, 16, pp. 1660-1672; 
Amrani, N., A faux 3{\textquoteright}-UTR promotes aberrant termination and triggers nonsense-mediated mRNA decay (2004) Nature, 432, pp. 112-118; 
Mintzer, M.A., Simanek, E.E., Nonviral vectors for gene delivery (2009) Chem. Rev., 109, pp. 259-302; 
Oliveira, H., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c (2010) J. Control. Release, 143, pp. 350-358; 
Duncan, R., Gaspar, R., Nanomedicine(s) under the microscope (2011) Mol. Pharm., 8, pp. 2101-2141; 
Zilberberg, A., Restoration of APC gene function in colorectal cancer cells by aminoglycoside- and macrolide-induced read-through of premature termination codons (2010) Gut, 59, pp. 496-507; 
Keeling, K.M., Bedwell, D.M., Clinically relevant aminoglycosides can suppress disease-associated premature stop mutations in the IDUA and P53 cDNAs in a mammalian translation system (2002) J. Mol. Med. (Berl.), 80, pp. 367-376; 
Floquet, C., Rescue of non-sense mutated p53 tumor suppressor gene by aminoglycosides (2011) Nucleic Acids Res., 39, pp. 3350-3362; 
Simoes-Correia, J., Endoplasmic reticulum quality control: a new mechanism of E-cadherin regulation and its implication in cancer (2008) Hum. Mol. Genet., 17, pp. 3566-3576; 
Mateus, A.R., E-cadherin mutations and cell motility: a genotype-phenotype correlation (2009) Exp. Cell Res., 315, pp. 1393-1402; 
Figueiredo, J., ADP-ribosylation factor 6 mediates E-cadherin recovery by chemical chaperones (2011) PLoS ONE, 6, pp. e23188; 
Demeshkina, N., A new understanding of the decoding principle on the ribosome (2012) Nature, 484, pp. 256-259; 
Wagner, K.R., Gentamicin treatment of Duchenne and Becker muscular dystrophy due to nonsense mutations (2001) Ann. Neurol., 49, pp. 706-711; 
Politano, L., Gentamicin administration in Duchenne patients with premature stop codon. Preliminary results (2003) Acta Myol., 22, pp. 15-21; 
Malik, V., Gentamicin-induced readthrough of stop codons in Duchenne muscular dystrophy (2010) Ann. Neurol., 67, pp. 771-780; 
Wilschanski, M., A pilot study of the effect of gentamicin on nasal potential difference measurements in cystic fibrosis patients carrying stop mutations (2000) Am. J. Respir. Crit. Care Med., 161, pp. 860-865; 
Clancy, J.P., Evidence that systemic gentamicin suppresses premature stop mutations in patients with cystic fibrosis (2001) Am. J. Respir. Crit. Care Med., 163, pp. 1683-1692; 
Wilschanski, M., Gentamicin-induced correction of CFTR function in patients with cystic fibrosis and CFTR stop mutations (2003) N. Engl. J. Med., 349, pp. 1433-1441; 
Clancy, J.P., No detectable improvements in cystic fibrosis transmembrane conductance regulator by nasal aminoglycosides in patients with cystic fibrosis with stop mutations (2007) Am. J. Respir. Cell Mol. Biol., 37, pp. 57-66; 
Sermet-Gaudelus, I., In vitro prediction of stop-codon suppression by intravenous gentamicin in patients with cystic fibrosis: a pilot study (2007) BMC Med., 5, p. 5; 
Kerem, E., Effectiveness of PTC124 treatment of cystic fibrosis caused by nonsense mutations: a prospective phase II trial (2008) Lancet, 372, pp. 719-727; 
Sermet-Gaudelus, I., Ataluren (PTC124) induces cystic fibrosis transmembrane conductance regulator protein expression and activity in children with nonsense mutation cystic fibrosis (2010) Am. J. Respir. Crit. Care Med., 182, pp. 1262-1272; 
Wilschanski, M., Chronic ataluren (PTC124) treatment of nonsense mutation cystic fibrosis (2011) Eur. Respir. J., 38, pp. 59-69; 
James, P.D., Aminoglycoside suppression of nonsense mutations in severe hemophilia (2005) Blood, 106, pp. 3043-3048; 
Pinotti, M., Gentamicin induces sub-therapeutic levels of coagulation factor VII in patients with nonsense mutations (2006) J. Thromb. Haemost., 4, pp. 1828-1830; 
Schroers, A., Gentamicin treatment in McArdle disease: failure to correct myophosphorylase deficiency (2006) Neurology, 66, pp. 285-286; 
Simon, A.J., The effect of gentamicin-induced readthrough on a novel premature termination codon of CD18 leukocyte adhesion deficiency patients (2010) PLoS ONE, 5, pp. e13659
},
	month = {2012///},
	pages = {667 - 678},
	abstract = {Several hereditary cancer syndromes are associated with nonsense mutations that create premature termination codons (PTC). Therapeutic strategies involving readthrough induction partially restore expression of proteins with normal function from nonsense-mutated genes, and small molecules such as aminoglycosides and PTC124 have exhibited promising results for treating patients with cystic fibrosis and Duchenne muscular dystrophy. Transgenic expression of suppressor-tRNAs and depleting translation termination factors are, among others, potential strategies for treating PTC-associated diseases. In this review, the potential of using readthrough strategies as a therapy for cancer syndromes is discussed, and we consider the effect of nonsense-mediated decay and other factors on readthrough efficiency. {\textcopyright} 2012 Elsevier Ltd.},
	keywords = {Aminoglycosides, Hereditary cancer syndromes, Nonsense-mediated decay (NMD), Nonsense-suppression, Premature termination codon (PTC), PTC124, Readthrough, Suppressor-tRNA},
	isbn = {14714914 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868212770\&partnerID=40\&md5=93ff561cb7d1c818f78a88ec1555181e},
	author = {Bordeira-Carri{\c c}o, R. and P{\^e}go, A.P. and Santos, M. and Oliveira, C.}
}
@booklet {2179,
	title = {Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII                    7-10)
},
	howpublished = {Acta BiomaterialiaActa Biomater.},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArticle in Pressdoi: 10.1016/j.actbio.2012.10.029Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 41email: iamaral@ineb.up.pt},
	month = {2012///},
	abstract = {The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4\% and 15\%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII                        7-10). Immobilized rhFNIII 
                        7-10 was characterized in terms of amount ( 
                        125I-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII 
                        7-10 with rhFNIII 
                        7-10 concentration, and, for the same concentration, higher amounts of rhFNIII 
                        7-10 on DA 4\% compared with DA 15\%. Moreover, rhFNIII 
                        7-10 concentrations as low as 5 and 20 μg ml 
},
	keywords = {Protein conformation, Protein radiolabelling, Spinal cord injury, Surface grafting, Three-dimensional scaffolds},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868680582\&partnerID=40\&md5=6599fd2565f4511b94e944978afc8073},
	author = {Amaral, I.F. and Neiva, I. and Ferreira da Silva, F. and Sousa, S.R. and Piloto, A.M. and Lopes, C.D.F. and Barbosa, M.A. and Kirkpatrick, C.J. and P{\^e}go, A.P.}
}
@article {2196,
	title = {A novel nanoparticle delivery system for in vivo targeting of the sciatic nerve: Impact on regeneration},
	journal = {NanomedicineNanomedicine},
	volume = {7},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.2217/nnm.11.188Language of Original Document: EnglishCorrespondence Address: Saraiva, M.J.; Unidade de Neurobiologia Molecular, IBMC-Instituto de Biologia Molecular e Celular, 4150-180 Porto, Portugal; email: mjsaraiv@ibmc.up.ptReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discovery Today: Disease Models, 1 (2), pp. 177-185. , DOI 10.1016/j.ddmod.2004.09.008;Apfel, S.C., Neurotrophic factors in peripheral neuropathies: Therapeutic implications (1999) Brain Pathology, 9 (2), pp. 393-413; 
Battiston, B., Geuna, S., Ferrero, M., Tos, P., Nerve repair by means of tubulization: Literature review and personal clinical experience comparing biological and synthetic conduits for sensory nerve repair (2005) Microsurgery, 25 (4), pp. 258-267. , DOI 10.1002/micr.20127; 
Madduri, S., Gander, B., Schwann cell delivery of neurotrophic factors for peripheral nerve regeneration (2010) J. Peripher. Nerv. Syst., 15, pp. 93-103; 
Kas, H.S., Chitosan: Properties, preparations and application to microparticulate systems (1997) Journal of Microencapsulation, 14 (6), pp. 689-711; 
Aimin, C., Chunlin, H., Juliang, B., Tinyin, Z., Zhichao, D., Antibiotic loaded chitosan bar: An in vitro, in vivo study of a possible treatment for osteomyelitis (1999) Clinical Orthopaedics and Related Research, (366), pp. 239-247; 
Nunthanid, J., Puttipipatkhachorn, S., Yamamoto, K., Peck, G.E., Physical properties and molecular behavior of chitosan films (2001) Drug Development and Industrial Pharmacy, 27 (2), pp. 143-157. , DOI 10.1081/DDC-100000481; 
Singla, A.K., Chawala, M., Chitosan: Some pharmaceutical and biological aspects-an update (2001) J. Pharm. Pharmacol., 53, pp. 67-147; 
Liu, Z., Jiao, Y., Liu, F., Zhang, Z., Heparin/chitosan nanoparticle carriers prepared by polyelectrolyte complexation (2007) Journal of Biomedical Materials Research - Part A, 83 (3), pp. 806-812. , DOI 10.1002/jbm.a.31407; 
Chavez-Delgado, M.E., Gomez-Pinedo, U., Feria-Velasco, A., Huerta-Viera, M., Castro Castaneda, S., Lopez-Dellamary Toral, F.A., Parducz, A., Garcia-Estrada, J., Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses (2005) Journal of Biomedical Materials Research - Part B Applied Biomaterials, 74 (1), pp. 589-600. , DOI 10.1002/jbm.b.30243; 
Li, X., Wang, W., Wei, G., Wang, G., Zhang, W., Ma, X., Immunophilin FK506 loaded in chitosan guides promotes peripheral nerve regeneration (2010) Biotechnol. Lett., 32 (9), pp. 1333-1337; 
Lin, Y.H., Chang, C.H., Wu, Y.S., Development of pH-responsive chitosan/ heparin nanoparticles for stomach-specific anti-Helicobacter pylori therapy (2009) Biomaterials, 30, pp. 3332-3342; 
Sakiyama-Elbert, S.E., Hubbell, J.A., Development of fibrin derivatives for controlled release of heparin- binding growth factors (2000) Journal of Controlled Release, 65 (3), pp. 389-402. , DOI 10.1016/S0168-3659(99)00221-7, PII S0168365999002217; 
Lee, A.C., Yu, V.M., Lowe III, J.B., Brenner, M.J., Hunter, D.A., Mackinnon, S.E., Sakiyama-Elbert, S.E., Controlled release of nerve growth factor enhances sciatic nerve regeneration (2003) Experimental Neurology, 184 (1), pp. 295-303. , DOI 10.1016/S0014-4886(03)00258-9; 
Ohta, M., Suzuki, Y., Chou, H., Ishikawa, N., Suzuki, S., Tanihara, M., Suzuki, Y., Ide, C., Novel heparin/alginate gel combined with basic fibroblast growth factor promotes nerve regeneration in rat sciatic nerve (2004) Journal of Biomedical Materials Research - Part A, 71 (4), pp. 661-668. , DOI 10.1002/jbm.a.30194; 
Chu, H., Johnson, N.R., Mason, N.S., Wang, Y.A., A [polycation:heparin] complex releases growth factors with enhanced bioactivity (2010) J. Control Release, 150 (2), pp. 157-163; 
Chau, C.H., Shum, D.K.Y., Chan, Y.S., So, K.-F., Heparan sulphates upregulate regeneration of transected sciatic nerves of adult guinea-pigs (1999) European Journal of Neuroscience, 11 (6), pp. 1914-1926. , DOI 10.1046/j.1460-9568.1999.00615.x; 
Dow, K.E., Riopelle, R.J., Kisilevsky, R., Domains of neuronal heparin sulphate proteoglycans involved in neurite growth on laminin (1991) Cell Tissue Res., 265, pp. 345-351; 
Gorio, A., Vergani, L., Ferro, L., Prino, G., Di Giulio, A.M., Glycosaminoglycans in nerve injury: Ii. Effects on transganglionic degeneration and on the expression of neurotrophic factors (1996) Journal of Neuroscience Research, 46 (5), pp. 572-580. , DOI 10.1002/(SICI)1097-4547(19961201)46:5<572::AID-JNR6>3.0.CO;2-G; 
Gorio, A., Lesma, E., Vergani, L., Di Giulio, A.M., Glycosaminoglycan supplementation promotes nerve regeneration and muscle reinnervation (1997) European Journal of Neuroscience, 9 (8), pp. 1748-1753. , DOI 10.1111/j.1460-9568.1997.tb01532.x; 
Boddohi, S., Moore, N., Johnson, P.A., Kipper, M.J., Polysaccharide-based polyelectrolyte complex nanoparticles from chitosan, heparin and hyaluronan (2009) Biomacromolecules, 10, pp. 1402-1409; 
Moreira, C., Oliveira, H., Pires, L.R., Sim{\~o}es, S., Barbosa, M.A., P{\^e}go, A.P., Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone (2009) Acta Biomaterialia, 5, pp. 2995-3006; 
De Medinaceli, L., Freed, W.J., Wyatt, R.J., An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks (1982) Experimental Neurology, 77 (3), pp. 634-643. , DOI 10.1016/0014-4886(82)90234-5; 
Bain, J.R., Mackinnon, S.E., Hunter, D.A., Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat (1989) Plastic and Reconstructive Surgery, 83 (1), pp. 129-136; 
Hare, G.M.T., Evans, P.J., Mackinnon, S.E., Walking track analysis: A long-term assessment of peripheral nerve recovery (1992) Plast. Reconstr. Surg., 89, pp. 251-258; 
Moghimi, S.M., Hunter, A.C., Murray, J.C., Nanomedicine: Current status and future prospects (2005) FASEB Journal, 19 (3), pp. 311-330. , DOI 10.1096/fj.04-2747rev; 
Xu, C., Inokuma, M.S., Denham, J., Golds, K., Kundu, P., Gold, J.D., Carpenter, M.K., Feeder-free growth of undifferentiated human embryonic stem cells (2001) Nature Biotechnology, 19 (10), pp. 971-974. , DOI 10.1038/nbt1001-971; 
Xu, X.M., Guenard, V., Kleitman, N., Bunge, M.B., Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord (1994) J. Comp. Neurol., 351 (1), pp. 145-160; 
Chen, M.C., Wong, H.S., Lin, K.J., The characteristics, biodistribution and bioavailability of a chitosan-based nanoparticulate system for the oral delivery of heparin (2009) Biomaterials, 30, pp. 6629-6637; 
Oyarzun-Ampuero, F.A., Brea, J., Loza, M.I., Torres, D., Alonso, M.J., Chitosan-hyaluronic acid nanoparticles loaded with heparin for the treatment of asthma (2009) Int. J. Pharm., 381 (2), pp. 122-129; 
Mao, H.-Q., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., August J.Thomas, Leong, K.W., Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency (2001) Journal of Controlled Release, 70 (3), pp. 399-421. , DOI 10.1016/S0168-3659(00)00361-8, PII S0168365900003618; 
Koping-Hoggard, M., Tubulekas, I., Guan, H., Edwards, K., Nilsson, M., Varum, K.M., Artursson, P., Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo (2001) Gene Therapy, 8 (14), pp. 1108-1121. , DOI 10.1038/sj.gt.3301492; 
Huang, M., Ma, Z., Khor, E., Lim, L.-Y., Uptake of FITC-chitosan nanoparticles by A549 cells (2002) Pharmaceutical Research, 19 (10), pp. 1488-1494. , DOI 10.1023/A:1020404615898; 
Fang, N., Chan, V., Mao, H.-Q., Leong, K.W., Interactions of phospholipid bilayer with chitosan: Effect of molecular weight and pH (2001) Biomacromolecules, 2 (4), pp. 1161-1168. , DOI 10.1021/bm015548s; 
Thanou, M., Verhoef, J.C., Junginger, H.E., Chitosan and its derivatives as intestinal absorption enhancers (2001) Adv. Drug Deliv. Rev., 50, pp. 91-101; 
Artursson, P., Lindmark, T., Davis, S.S., Illum, L., Effect of chitosan on the permeability of monolayers of intestinal epithelial cells (Caco-2) (1994) Pharmaceutical Research, 11 (9), pp. 1358-1361. , DOI 10.1023/A:1018967116988; 
Bowman, K., Leong, K.W., Chitosan nanoparticles for oral drug and gene delivery (2006) Int. J. Nanomed., 1 (2), pp. 117-128; 
Agnihotri, S.A., Mallikarjuna, N.N., Aminabhavi, T.M., Recent advances on chitosan-based micro- and nanoparticles in drug delivery (2004) Journal of Controlled Release, 100 (1), pp. 5-28. , DOI 10.1016/j.jconrel.2004.08.010, PII S0168365904003803; 
Mittnacht, U., Hartmann, H., Hein, S., Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth (2010) Nano Lett., 10 (10), pp. 3933-3939; 
Oliveira, H., Pires, L.R., Fernandez, R., Martins, M.C., Sim{\~o}es, S., P{\^e}go, A.P., Chitosan-based gene delivery vectors targeted to the peripheral nervous system (2010) J. Biomed. Mater. Res., A95 (3), pp. 801-810; 
Kofuji, K., Ito, T., Murata, Y., Kawashima, S., Effect of chondroitin sulfate on the biodegradation and drug release of chitosan gel beads in subcutaneous air pouches of mice (2002) Biological and Pharmaceutical Bulletin, 25 (2), pp. 268-271. , DOI 10.1248/bpb.25.268; 
Pires, L.R., Oliveira, H., Barrias, C.C., Imidazole-grafted chitosan-mediated gene delivery: in vitro study on transfection, intracellular trafficking and degradation (2011) Nanomedicine (Lond., 6 (9), pp. 1499-1512; 
Baldrick, P., The safety of chitosan as a pharmaceutical excipient (2010) Regul. Toxicol. Pharmacol., 56 (3), pp. 290-299; 
Pavinatto, F.J., Pavinatto, A., Caseli, L., Dos Santos Jr., D.S., Nobre, T.M., Zaniquelli, M.E.D., Oliveira Jr., O.N., Interaction of chitosan with cell membrane models at thr air-water interface (2007) Biomacromolecules, 8 (5), pp. 1633-1640. , DOI 10.1021/bm0701550; 
Sorokin, L., The impact of the extracellular matrix on inflammation (2010) Nat. Rev. Immunol., 10 (10), pp. 712-723; 
Roghani, M., Mansukhani, A., Dell{\textquoteright}Era, P., Bellosta, P., Basilico, C., Rifkin, D.B., Moscatelli, D., Heparin increases the affinity of basic fibroblast growth factor for its receptor but is not required for binding (1994) Journal of Biological Chemistry, 269 (6), pp. 3976-3984
},
	month = {2012///},
	pages = {1167 - 1180},
	abstract = {Aim: Innovative solutions in the development of drug delivery systems targeting the nerve tissue are awaited. In this regard, a novel system for the delivery of drugs to the sciatic nerve was created using nanomedical principles. Materials \& methods: Chitosan was the vehicle material used in the experiment. Heparin bound to growth factors has been administered to enhance peripheral nerve regeneration, and since heparin possesses the appropriate charge to be able to form nanoparticles with chitosan, it appears to be a good candidate to base this new delivery system on. Results: Maximal absorption took place throughout the extracellular matrix at day 15. No major inflammatory response was observed, indicating that this is a safe and biocompatible system for drug delivery to nerves. Sensorimotor performance and nerve regeneration of mice receiving these nanoparticles were superior as compared with controls. Conclusion: Our work demonstrates a versatile nanoparticle delivery system that successfully targets drugs {\textquoteright}in vivo to the sciatic nerve, opening novel avenues in the field of nanomedicine to the design of therapeutic strategies that enhance axonal regeneration. {\textcopyright} 2012 Future Medicine Ltd.},
	keywords = {chitosan, drug delivery system, heparin, nanomedicine, nanoparticles, peripheral nerve regeneration, sciatic nerve},
	isbn = {17435889 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868111412\&partnerID=40\&md5=5db0c76b3a921ca70565c9f3f63cf004},
	author = {Gon{\c c}alves, N.P. and Oliveira, H. and P{\^e}go, A.P. and Saraiva, M.J.}
}
@article {2184,
	title = {Regenerative medicine for the treatment of spinal cord injury: More than just promises?},
	journal = {Journal of Cellular and Molecular MedicineJ. Cell. Mol. Med.},
	volume = {16},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.1111/j.1582-4934.2012.01603.xLanguage of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Fitch, M.T., Silver, J., CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure (2008) Exp Neurol, 209, pp. 294-301;Ronaghi, M., Erceg, S., Moreno-Manzano, V., Challenges of stem cell therapy for spinal cord injury: human embryonic stem cells, endogenous neural stem cells, or induced pluripotent stem cells? (2010) Stem Cells, 28, pp. 93-99; 
Wyndaele, M., Wyndaele, J.J., Incidence, prevalence and epidemiology of spinal cord injury: what learns a worldwide literature survey? (2006) Spinal Cord, 44, pp. 523-529; 
Willerth, S.M., Sakiyama-Elbert, S.E., Cell therapy for spinal cord regeneration (2008) Adv Drug Deliv Rev, 60, pp. 263-276; 
Hulsebosch, C.E., Recent advances in pathophysiology and treatment of spinal cord injury (2002) Adv Physiol Educ, 26, pp. 238-255; 
Fawcett, J.W., Overcoming inhibition in the damaged spinal cord (2006) J Neurotrauma, 23, pp. 371-383; 
Garcia-Alias, G., Lin, R., Akrimi, S.F., Therapeutic time window for the application of chondroitinase ABC after spinal cord injury (2008) Exp Neurol, 210, pp. 331-338; 
Muir, E.M., Fyfe, I., Gardiner, S., Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells (2010) J Biotechnol, 145, pp. 103-110; 
Barnabe-Heider, F., Frisen, J., Stem cells for spinal cord repair (2008) Cell Stem Cell, 3, pp. 16-24; 
Bradbury, E.J., McMahon, S.B., Spinal cord repair strategies: why do they work? (2006) Nat Rev Neurosci, 7, pp. 644-653; 
Xu, X.M., Onifer, S.M., Transplantation-mediated strategies to promote axonal regeneration following spinal cord injury (2009) Respir Physiol Neurobiol, 169, pp. 171-182; 
Sykova, E., Homola, A., Mazanec, R., Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury (2006) Cell Transplant, 15, pp. 675-687; 
Wright, K.T., Masri, W.E., Osman, A., Concise review: bone marrow for the treatment of spinal cord injury: mechanisms and clinical applications (2011) Stem Cells, 29, pp. 169-178; 
Cizkova, D., Kakinohana, O., Kucharova, K., Functional recovery in rats with ischemic paraplegia after spinal grafting of human spinal stem cells (2007) Neuroscience, 147, pp. 546-560; 
Hofstetter, C.P., Schwarz, E.J., Hess, D., Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery (2002) Proc Natl Acad Sci USA, 99, pp. 2199-2204; 
Cizkova, D., Nagyova, M., Slovinska, L., Response of ependymal progenitors to spinal cord injury or enhanced physical activity in adult rat (2009) Cell Mol Neurobiol, 29, pp. 999-1013; 
Barnabe-Heider, F., Goritz, C., Sabelstrom, H., Origin of new glial cells in intact and injured adult spinal cord (2010) Cell Stem Cell, 7, pp. 470-482; 
Sykova, E., Jendelova, P., Magnetic resonance tracking of implanted adult and embryonic stem cells in injured brain and spinal cord (2005) Ann N Y Acad Sci, pp. 146-160; 
Kubinova, S., Sykova, E., Nanotechnology for treatment of stroke and spinal cord injury (2010) Nanomedicine-UK, 5, pp. 99-108; 
Madigan, N.N., McMahon, S., O{\textquoteright}Brien, T., Current tissue engineering and novel therapeutic approaches to axonal regeneration following spinal cord injury using polymer scaffolds (2009) Respir Physiol Neurobiol, 169, pp. 183-199; 
Blits, B., Boer, G.J., Verhaagen, J., Pharmacological, cell, and gene therapy strategies to promote spinal cord regeneration (2002) Cell Transplant, 11, pp. 593-613; 
Tetzlaff, W., Okon, E.B., Karimi-Abdolrezaee, S., A systematic review of cellular transplantation therapies for spinal cord injury (2011) J Neurotrauma, 28, pp. 1611-1682; 
Sykova, E., Jendelova, P., Urdzikova, L., Bone marrow stem cells and polymer hydrogels-two strategies for spinal cord injury repair (2006) Cell Mol Neurobiol, 26, pp. 1113-1129; 
Papastefanaki, F., Chen, J., Lavdas, A.A., Grafts of Schwann cells engineered to express PSA-NCAM promote functional recovery after spinal cord injury (2007) Brain, 130, pp. 2159-2174; 
Raisman, G., Repair of spinal cord injury by transplantation of olfactory ensheathing cells (2007) C R Biol, 330, pp. 557-560; 
Takahashi, K., Yamanaka, S., Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors (2006) Cell, 126, pp. 663-676; 
Okita, K., Ichisaka, T., Yamanaka, S., Generation of germline-competent induced pluripotent stem cells (2007) Nature, 448, pp. 313-317; 
Zhao, T., Zhang, Z.N., Rong, Z., Immunogenicity of induced pluripotent stem cells (2011) Nature, 474, pp. 212-215; 
Thomson, J.A., Odorico, J.S., Human embryonic stem cell and embryonic germ cell lines (2000) Trends Biotechnol, 18, pp. 53-57; 
Zeng, X., Rao, M.S., Human embryonic stem cells: long term stability, absence of senescence and a potential cell source for neural replacement (2007) Neuroscience, 145, pp. 1348-1358; 
Holubcova, Z., Matula, P., Sedlackova, M., Human embryonic stem cells suffer from centrosomal amplification (2011) Stem Cells, 29, pp. 46-56; 
Liu, S., Qu, Y., Stewart, T.J., Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation (2000) Proc Natl Acad Sci USA, 97, pp. 6126-6131; 
Kakinohana, O., Cizkova, D., Tomori, Z., Region-specific cell grafting into cervical and lumbar spinal cord in rat: a qualitative and quantitative stereological study (2004) Exp Neurol, 190, pp. 122-132; 
Lang, K.J., Rathjen, J., Vassilieva, S., Differentiation of embryonic stem cells to a neural fate: a route to re-building the nervous system? (2004) J Neurosci Res, 76, pp. 184-192; 
Carpenter, M.K., Inokuma, M.S., Denham, J., Enrichment of neurons and neural precursors from human embryonic stem cells (2001) Exp Neurol, 172, pp. 383-397; 
Li, X.J., Du, Z.W., Zarnowska, E.D., Specification of motoneurons from human embryonic stem cells (2005) Nat Biotechnol, 23, pp. 215-221; 
Keirstead, H.S., Nistor, G., Bernal, G., Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury (2005) J Neurosci, 25, pp. 4694-4705; 
Seminatore, C., Polentes, J., Ellman, D., The postischemic environment differentially impacts teratoma or tumor formation after transplantation of human embryonic stem cell-derived neural progenitors (2010) Stroke, 41, pp. 153-159; 
Faulkner, J., Keirstead, H.S., Human embryonic stem cell-derived oligodendrocyte progenitors for the treatment of spinal cord injury (2005) Transpl Immunol, 15, pp. 131-142; 
Nistor, G.I., Totoiu, M.O., Haque, N., Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation (2005) Glia, 49, pp. 385-396; 
Cloutier, F., Siegenthaler, M.M., Nistor, G., Transplantation of human embryonic stem cell-derived oligodendrocyte progenitors into rat spinal cord injuries does not cause harm (2006) Regen Med, 1, pp. 469-479; 
Altman, J., Are new neurons formed in the brains of adult mammals? (1962) Science, 135, pp. 1127-1128; 
Gage, F.H., Mammalian neural stem cells (2000) Science, 287, pp. 1433-1438; 
Goldman, S., Stem and progenitor cell-based therapy of the human central nervous system (2005) Nat Biotechnol, 23, pp. 862-871; 
Horky, L.L., Galimi, F., Gage, F.H., Fate of endogenous stem/progenitor cells following spinal cord injury (2006) J Comp Neurol, 498, pp. 525-538; 
Setoguchi, T., Nakashima, K., Takizawa, T., Treatment of spinal cord injury by transplantation of fetal neural precursor cells engineered to express BMP inhibitor (2004) Exp Neurol, 189, pp. 33-44; 
Cizkova, D., Cizek, M., Nagyova, M., Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting (2009) J Neurosci Meth, 184, pp. 88-94; 
Lu, P., Jones, L.L., Snyder, E.Y., Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury (2003) Exp Neurol, 181, pp. 115-129; 
Ishii, K., Nakamura, M., Dai, H., Neutralization of ciliary neurotrophic factor reduces astrocyte production from transplanted neural stem cells and promotes regeneration of corticospinal tract fibers in spinal cord injury (2006) J Neurosci Res, 84, pp. 1669-1681; 
Tarasenko, Y.I., Gao, J., Nie, L., Human fetal neural stem cells grafted into contusion-injured rat spinal cords improve behavior (2007) J Neurosci Res, 85, pp. 47-57; 
Cummings, B.J., Uchida, N., Tamaki, S.J., Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice (2005) Proc Natl Acad Sci USA, 102, pp. 14069-14074; 
Marsala, M., Kakinohana, O., Yaksh, T.L., Spinal implantation of hNT neurons and neuronal precursors: graft survival and functional effects in rats with ischemic spastic paraplegia (2004) Eur J Neurosci, 20, pp. 2401-2414; 
Wu, R., Wang, H., Xia, X., Nerve injection of viral vectors efficiently transfers transgenes into motor neurons and delivers RNAi therapy against ALS (2009) Antioxid Redox Signal, 11, pp. 1523-1534; 
Romanyuk, N., Amemori, T., Turnovcova, K., (2011), Using human fetal neural stem cells or human induced pluripotent stem cell-derived neural precursors for the treatment of experimental spinal cord injury. 8th IBRO World Congress of Neuroscience. Florence, ItalyHooshmand, M.J., Sontag, C.J., Uchida, N., Analysis of host-mediated repair mechanisms after human CNS-stem cell transplantation for spinal cord injury: correlation of engraftment with recovery (2009) PLoS ONE, 4, pp. e5871; 
Hofstetter, C.P., Holmstrom, N.A., Lilja, J.A., Allodynia limits the usefulness of intraspinal neural stem cell grafts; directed differentiation improves outcome (2005) Nature Neurosci, 8, pp. 346-353; 
Mukhida, K., Mendez, I., McLeod, M., Spinal GABAergic transplants attenuate mechanical allodynia in a rat model of neuropathic pain (2007) Stem Cells, 25, pp. 2874-2885; 
Takami, T., Oudega, M., Bates, M.L., Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord (2002) J Neurosci, 22, pp. 6670-6681; 
Pinzon, A., Calancie, B., Oudega, M., Conduction of impulses by axons regenerated in a Schwann cell graft in the transected adult rat thoracic spinal cord (2001) J Neurosci Res, 64, pp. 533-541; 
Guest, J.D., Hiester, E.D., Bunge, R.P., Demyelination and Schwann cell responses adjacent to injury epicenter cavities following chronic human spinal cord injury (2005) Exp Neurol, 192, pp. 384-393; 
Pearse, D.D., Sanchez, A.R., Pereira, F.C., Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery (2007) Glia, 55, pp. 976-1000; 
Bunge, M.B., Novel combination strategies to repair the injured mammalian spinal cord (2008) J Spinal Cord Med, 31, pp. 262-269; 
Lu, P., Tuszynski, M.H., Growth factors and combinatorial therapies for CNS regeneration (2008) Exp Neurol, 209, pp. 313-320; 
Afshari, F.T., Kwok, J.C., Fawcett, J.W., Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling (2010) J Neurosci, 30, pp. 4246-4255; 
Mackay-Sim, A., Olfactory ensheathing cells and spinal cord repair (2005) Keio J Med, 54, pp. 8-14; 
Feron, F., Perry, C., Cochrane, J., Autologous olfactory ensheathing cell transplantation in human spinal cord injury (2005) Brain, 128, pp. 2951-2960; 
Lima, C., Pratas-Vital, J., Escada, P., Olfactory mucosa autografts in human spinal cord injury: a pilot clinical study (2006) J Spinal Cord Med, 29, pp. 191-203; 
Mackay-Sim, A., Feron, F., Cochrane, J., Autologous olfactory ensheathing cell transplantation in human paraplegia: a 3-year clinical trial (2008) Brain, 131, pp. 2376-2386; 
Fouad, K., Schnell, L., Bunge, M.B., Combining Schwann cell bridges and olfactory-ensheathing glia grafts with chondroitinase promotes locomotor recovery after complete transection of the spinal cord (2005) J Neurosci, 25, pp. 1169-1178; 
Amemori, T., Jendelova, P., Ruzickova, K., Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat (2010) Cytotherapy, 12, pp. 212-225; 
Salem, H.K., Thiemermann, C., Mesenchymal stromal cells: current understanding and clinical status (2010) Stem Cells, 28, pp. 585-596; 
Cizkova, D., Rosocha, J., Vanicky, I., Transplants of human mesenchymal stem cells improve functional recovery after spinal cord injury in the rat (2006) Cell Mol Neurobiol, 26, pp. 1167-1180; 
Sykova, E., Jendelova, P., Migration, fate and in vivo imaging of adult stem cells in the CNS (2007) Cell Death Differ, 14, pp. 1336-1342; 
Urdzikova, L., Jendelova, P., Glogarova, K., Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats (2006) J Neurotrauma, 23, pp. 1379-1391; 
Peran, M., Marchal, J.A., Rodriguez-Serrano, F., Transdifferentiation: why and how? (2011) Cell Biol Int, 35, pp. 373-379; 
Lambert, A.P.F., Zandonai, A.F., Bonatto, D., Differentiation of human adipose-derived adult stem cells into neuronal tissue: Does it work? (2009) Differentiation, 77, pp. 221-228; 
Uccelli, A., Benvenuto, F., Laroni, A., Neuroprotective features of mesenchymal stem cells (2011) Best Pract Res Cl Ha, 24, pp. 59-64; 
Geffner, L.F., Santacruz, P., Izurieta, M., Administration of autologous bone marrow stem cells into spinal cord injury patients via multiple routes is safe and improves their quality of life: comprehensive case studies (2008) Cell Transplant, 17, pp. 1277-1293; 
Park, H.C., Shim, Y.S., Ha, Y., Treatment of complete spinal cord injury patients by autologous bone marrow cell transplantation and administration of granulocyte-macrophage colony stimulating factor (2005) Tissue Eng, 11, pp. 913-922; 
Yoon, S.H., Shim, Y.S., Park, Y.H., Complete spinal cord injury treatment using autologous bone marrow cell transplantation and bone marrow stimulation with granulocyte macrophage-colony stimulating factor: Phase I/II clinical trial (2007) Stem Cells, 25, pp. 2066-2073; 
Callera, F., do Nascimento, R.X., Delivery of autologous bone marrow precursor cells into the spinal cord via lumbar puncture technique in patients with spinal cord injury: a preliminary safety study (2006) Exp Hematol, 34, pp. 130-131; 
Deda, H., Inci, M.C., Kurekci, A.E., Treatment of chronic spinal cord injured patients with autologous bone marrow-derived hematopoietic stem cell transplantation: 1-year follow-up (2008) Cytotherapy, 10, pp. 565-574; 
Saito, F., Nakatani, T., Iwase, M., Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation: the first clinical trial case report (2008) J Trauma, 64, pp. 53-59; 
Pal, R., Venkataramana, N.K., Bansal, A., Ex vivo-expanded autologous bone marrow-derived mesenchymal stromal cells in human spinal cord injury/paraplegia: a pilot clinical study (2009) Cytotherapy, 11, pp. 897-911; 
Mason, M.R., Ehlert, E.M., Eggers, R., Comparison of AAV serotypes for gene delivery to dorsal root ganglion neurons (2010) Mol Ther, 18, pp. 715-724; 
Petruska, J.C., Kitay, B., Boyce, V.S., Intramuscular AAV delivery of NT-3 alters synaptic transmission to motoneurons in adult rats (2010) Eur J Neurosci, 32, pp. 997-1005; 
Tuszynski, M.H., Nerve growth factor gene delivery: animal models to clinical trials (2007) Dev Neurobiol, 67, pp. 1204-1215; 
Blits, B., Derks, S., Twisk, J., Adeno-associated viral vector (AAV)-mediated gene transfer in the red nucleus of the adult rat brain: comparative analysis of the transduction properties of seven AAV serotypes and lentiviral vectors (2010) J Neurosci Meth, 185, pp. 257-263; 
Aalbers, C.J., Tak, P.P., Vervoordeldonk, M.J., Advancements in adeno-associated viral gene therapy approaches: exploring a new horizon (2011) F1000 Med Reports, 3, p. 17; 
Straley, K.S., Foo, C.W.P., Heilshorn, S.C., Biomaterial design strategies for the treatment of spinal cord injuries (2010) J Neurotrauma, 27, pp. 1-19; 
Hejcl, A., Lesny, P., Pradny, M., Biocompatible hydrogels in spinal cord injury repair (2008) Physiol Res, 57, pp. S121-S132; 
Friedman, J.A., Windebank, A.J., Moore, M.J., Biodegradable polymer grafts for surgical repair of the injured spinal cord (2002) Neurosurgery, 51, pp. 742-752; 
De Laporte, L., Lei Yan, A., Shea, L.D., Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury (2009) Biomaterials, 30, pp. 2361-2368; 
Wong, D.Y., Leveque, J.C., Brumblay, H., Macro-architectures in spinal cord scaffold implants influence regeneration (2008) J Neurotrauma, 25, pp. 1027-1037; 
P{\^e}go, A.P., Poot, A.A., Grijpma, D.W., Copolymers of trimethylene carbonate and epsilon-caprolactone for porous nerve guides: Synthesis and properties (2001) J Biomat Sci-Polym E, 12, pp. 35-53; 
Tsai, E.C., Dalton, P.D., Shoichet, M.S., Synthetic hydrogel guidance channels facilitate regeneration of adult rat brainstem motor axons after complete spinal cord transection (2004) J Neurotrauma, 21, pp. 789-804; 
Woerly, S., Fort, S., Pignot-Paintrand, I., Development of a sialic acid-containing hydrogel of poly[N-(2-hydroxypropyl) methacrylamide]: characterization and implantation study (2008) Biomacromolecules, 9, pp. 2329-2337; 
Collazos-Castro, J.E., Polo, J.L., Hernandez-Labrado, G.R., Bioelectrochemical control of neural cell development on conducting polymers (2010) Biomaterials, 31, pp. 9244-9255; 
Ciardelli, G., Chiono, V., Materials for peripheral nerve regeneration (2006) Macromol Biosci, 6, pp. 13-26; 
Yoshii, S., Ito, S., Shima, M., Functional restoration of rabbit spinal cord using collagen-filament scaffold (2009) J Tissue Eng Regen M, 3, pp. 19-25; 
Taylor, S.J., McDonald III, J.W., Sakiyama-Elbert, S.E., Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury (2004) J Control Release, 98, pp. 281-294; 
P{\^e}go, A.P., Mar, F.M., Rocha, D.N., Scaffold-driven regenerative therapy for the spinal cord injury - biomimeting neurogenesis in the CNS (2011) Histol Histopathol, 26, p. 289; 
Wei, Y.T., He, Y., Xu, C.L., Hyaluronic acid hydrogel modified with nogo-66 receptor antibody and poly-L-lysine to promote axon regrowth after spinal cord injury (2010) J Biomed Mater Res B, 95, pp. 110-117; 
Park, J., Lim, E., Back, S., Nerve regeneration following spinal cord injury using matrix metalloproteinase-sensitive, hyaluronic acid-based biomimetic hydrogel scaffold containing brain-derived neurotrophic factor (2010) J Biomed Mater Res A, 93, pp. 1091-1099; 
Gros, T., Sakamoto, J.S., Blesch, A., Regeneration of long-tract axons through sites of spinal cord injury using templated agarose scaffolds (2010) Biomaterials, 31, pp. 6719-6729; 
Prang, P., Muller, R., Eljaouhari, A., The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels (2006) Biomaterials, 27, pp. 3560-3569; 
Bozkurt, G., Mothe, A.J., Zahir, T., Chitosan channels containing spinal cord-derived stem/progenitor cells for repair of subacute spinal cord injury in the rat (2010) Neurosurgery, 67, pp. 1733-1744; 
Shen, Y., Qian, Y., Zhang, H., Guidance of olfactory ensheathing cell growth and migration on electrospun silk fibroin scaffolds (2010) Cell Transplant, 19, pp. 147-157; 
Novikova, L.N., Pettersson, J., Brohlin, M., Biodegradable poly-beta-hydroxybutyrate scaffold seeded with Schwann cells to promote spinal cord repair (2008) Biomaterials, 29, pp. 1198-1206; 
Bhattarai, N., Li, Z., Gunn, J., Natural-synthetic polyblend nanofibers for biomedical applications (2009) Adv Mater, 21, pp. 2792-2797; 
Hejcl, A., Lesny, P., Pradny, M., Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 6: 3D hydrogels with positive and negative surface charges and polyelectrolyte complexes in spinal cord injury repair (2009) J Mater Sci Mater Med, 20, pp. 1571-1577; 
Kubinova, S., Horak, D., Sykova, E., Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering (2009) Biomaterials, 30, pp. 4601-4609; 
Kubinova, S., Horak, D., Hejcl, A., Highly superporous cholesterol-modified poly(2-hydroxyethyl methacrylate) scaffolds for spinal cord injury repair (2011) J Biomed Mater Res A, 99, pp. 618-629; 
Gunn, J.W., Turner, S.D., Mann, B.K., Adhesive and mechanical properties of hydrogels influence neurite extension (2005) J Biomed Mater Res A, 72, pp. 91-97; 
Kubinova, S., Horak, D., Kozubenko, N., The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors (2010) Biomaterials, 31, pp. 5966-5975; 
Cui, H., Webber, M.J., Stupp, S.I., Self-assembly of peptide amphiphiles: from molecules to nanostructures to biomaterials (2010) Biopolymers, 94, pp. 1-18; 
Tysseling-Mattiace, V.M., Sahni, V., Niece, K.L., Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury (2008) J Neurosci, 28, pp. 3814-3823; 
Bellamkonda, R., Ranieri, J.P., Bouche, N., Hydrogel-based three-dimensional matrix for neural cells (1995) J Biomed Mater Res, 29, pp. 663-671; 
Wang, M., Zhai, P., Chen, X., Bioengineered scaffolds for spinal cord repair (2011) Tissue Eng Pt B-Rev, 17, pp. 177-194; 
Stokols, S., Sakamoto, J., Breckon, C., Templated agarose scaffolds support linear axonal regeneration (2006) Tissue Eng, 12, pp. 2777-2787; 
Stokols, S., Tuszynski, M.H., Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury (2006) Biomaterials, 27, pp. 443-451; 
Moore, M.J., Friedman, J.A., Lewellyn, E.B., Multiple-channel scaffolds to promote spinal cord axon regeneration (2006) Biomaterials, 27, pp. 419-429; 
Wang, H.B., Mullins, M.E., Cregg, J.M., Creation of highly aligned electrospun poly-L-lactic acid fibers for nerve regeneration applications (2009) J Neural Eng, 6, p. 016001; 
Wang, H.B., Mullins, M.E., Cregg, J.M., Varying the diameter of aligned electrospun fibers alters neurite outgrowth and Schwann cell migration (2010) Acta Biomater, 6, pp. 2970-2978; 
Xie, J., MacEwan, M.R., Li, X., Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties (2009) ACS Nano, 3, pp. 1151-1159; 
Hurtado, A., Cregg, J.M., Wang, H.B., Robust CNS regeneration after complete spinal cord transection using aligned poly-l-lactic acid microfibers (2011) Biomaterials, 32, pp. 6068-6079; 
Guo, J., Su, H., Zeng, Y., Reknitting the injured spinal cord by self-assembling peptide nanofiber scaffold (2007) Nanomedicine, 3, pp. 311-321; 
Johnson, P.J., Tatara, A., McCreedy, D.A., Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI (2010) Soft Matter, 6, pp. 5127-5137; 
Olson, H.E., Rooney, G.E., Gross, L., Neural stem cell- and Schwann cell-loaded biodegradable polymer scaffolds support axonal regeneration in the transected spinal cord (2009) Tissue Eng Pt A, 15, pp. 1797-1805; 
Hurtado, A., Moon, L.D., Maquet, V., Poly (D, L-lactic acid) macroporous guidance scaffolds seeded with Schwann cells genetically modified to secrete a bi-functional neurotrophin implanted in the completely transected adult rat thoracic spinal cord (2006) Biomaterials, 27, pp. 430-442; 
Woerly, S., Pinet, E., de Robertis, L., Spinal cord repair with PHPMA hydrogel containing RGD peptides (NeuroGel) (2001) Biomaterials, 22, pp. 1095-1111; 
Loh, N.K., Woerly, S., Bunt, S.M., The regrowth of axons within tissue defects in the CNS is promoted by implanted hydrogel matrices that contain BDNF and CNTF producing fibroblasts (2001) Exp Neurol, 170, pp. 72-84; 
Hejcl, A., Sedy, J., Kapcalova, M., HPMA-RGD hydrogels seeded with mesenchymal stem cells improve functional outcome in chronic spinal cord injury (2010) Stem Cells Dev, 19, pp. 1535-1546; 
Willerth, S.M., Sakiyama-Elbert, S.E., Approaches to neural tissue engineering using scaffolds for drug delivery (2007) Adv Drug Deliv Rev, 59, pp. 325-338; 
Jimenez Hamann, M.C., Tator, C.H., Shoichet, M.S., Injectable intrathecal delivery system for localized administration of EGF and FGF-2 to the injured rat spinal cord (2005) Exp Neurol, 194, pp. 106-119; 
Jain, A., Kim, Y.T., McKeon, R.J., In situ gelling hydrogels for conformal repair of spinal cord defects, and local delivery of BDNF after spinal cord injury (2006) Biomaterials, 27, pp. 497-504; 
Piantino, J., Burdick, J.A., Goldberg, D., An injectable, biodegradable hydrogel for trophic factor delivery enhances axonal rewiring and improves performance after spinal cord injury (2006) Exp Neurol, 201, pp. 359-367; 
Hendriks, W.T., Ruitenberg, M.J., Blits, B., Viral vector-mediated gene transfer of neurotrophins to promote regeneration of the injured spinal cord (2004) Prog Brain Res, 146, pp. 451-476; 
Abdellatif, A.A., Pelt, J.L., Benton, R.L., Gene delivery to the spinal cord: comparison between lentiviral, adenoviral, and retroviral vector delivery systems (2006) J Neurosci Res, 84, pp. 553-567; 
Blits, B., Bunge, M.B., Direct gene therapy for repair of the spinal cord (2006) J Neurotrauma, 23, pp. 508-520; 
Oliveira, H., Fernandez, R., Pires, L.R., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c (2010) J Control Release, 143, pp. 350-358; 
Oliveira, H., Pires, L.R., Fernandez, R., Chitosan-based gene delivery vectors targeted to the peripheral nervous system (2010) J Biomed Mater Res A, 95, pp. 801-810; 
Whitehead, K.A., Langer, R., Anderson, D.G., Knocking down barriers: advances in siRNA delivery (2009) Nat Rev Drug Discov, 8, pp. 129-138; 
Mittnacht, U., Hartmann, H., Hein, S., Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth (2010) Nano Lett, 10, pp. 3933-3939; 
Richardson, P.M., McGuinness, U.M., Aguayo, A.J., Axons from CNS neurons regenerate into PNS grafts (1980) Nature, 284, pp. 264-265; 
David, S., Aguayo, A.J., Axonal elongation into peripheral nervous system "bridges" after central nervous system injury in adult rats (1981) Science, 214, pp. 931-933; 
Schweigreiter, R., The natural history of the myelin-derived nerve growth inhibitor Nogo-A (2008) Neuron Glia Biol, 4, pp. 83-89; 
Vargas, M.E., Barres, B.A., Why is Wallerian degeneration in the CNS so slow? (2007) Annu Rev Neurosci, 30, pp. 153-179; 
Neumann, S., Woolf, C.J., Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury (1999) Neuron, 23, pp. 83-91; 
Ylera, B., Erturk, A., Hellal, F., Chronically CNS-injured adult sensory neurons gain regenerative competence upon a lesion of their peripheral axon (2009) Curr Biol, 19, pp. 930-936; 
Qiu, J., Cai, D., Dai, H., Spinal axon regeneration induced by elevation of cyclic AMP (2002) Neuron, 34, pp. 895-903; 
Jacob, J.M., Croes, S.A., Acceleration of axonal outgrowth in motor axons from mature and old F344 rats after a conditioning lesion (1998) Exp Neurol, 152, pp. 231-237; 
McQuarrie, I.G., Grafstein, B., Effect of a conditioning lesion on optic nerve regeneration in goldfish (1981) Brain Res, 216, pp. 253-264; 
Neumann, S., Bradke, F., Tessier-Lavigne, M., Regeneration of sensory axons within the injured spinal cord induced by intraganglionic cAMP elevation (2002) Neuron, 34, pp. 885-893; 
Zou, H., Ho, C., Wong, K., Axotomy-induced Smad1 activation promotes axonal growth in adult sensory neurons (2009) J Neurosci, 29, pp. 7116-7123; 
Seijffers, R., Mills, C.D., Woolf, C.J., ATF3 increases the intrinsic growth state of DRG neurons to enhance peripheral nerve regeneration (2007) J Neurosci, 27, pp. 7911-7920; 
Cafferty, W.B., Gardiner, N.J., Gavazzi, I., Leukemia inhibitory factor determines the growth status of injured adult sensory neurons (2001) J Neurosci, 21, pp. 7161-7170; 
Cafferty, W.B., Gardiner, N.J., Das, P., Conditioning injury-induced spinal axon regeneration fails in interleukin-6 knock-out mice (2004) J Neurosci, 24, pp. 4432-4443; 
Cao, Z., Gao, Y., Bryson, J.B., The cytokine interleukin-6 is sufficient but not necessary to mimic the peripheral conditioning lesion effect on axonal growth (2006) J Neurosci, 26, pp. 5565-5573; 
Minor, K., Phillips, J., Seeds, N.W., Tissue plasminogen activator promotes axonal outgrowth on CNS myelin after conditioned injury (2009) J Neurochem, 109, pp. 706-715; 
Pearse, D.D., Pereira, F.C., Marcillo, A.E., cAMP and Schwann cells promote axonal growth and functional recovery after spinal cord injury (2004) Nat Med, 10, pp. 610-616; 
Nikulina, E., Tidwell, J.L., Dai, H.N., The phosphodiesterase inhibitor rolipram delivered after a spinal cord lesion promotes axonal regeneration and functional recovery (2004) Proc Natl Acad Sci USA, 101, pp. 8786-8790; 
Cai, D., Deng, K., Mellado, W., Arginase I and polyamines act downstream from cyclic AMP in overcoming inhibition of axonal growth MAG and myelin in vitro (2002) Neuron, 35, pp. 711-719; 
Ma, T.C., Campana, A., Lange, P.S., A large-scale chemical screen for regulators of the arginase 1 promoter identifies the soy isoflavone daidzeinas a clinically approved small molecule that can promote neuronal protection or regeneration via a cAMP-independent pathway (2010) J Neurosci, 30, pp. 739-748; 
Bregman, B.S., Kunkel-Bagden, E., Schnell, L., Recovery from spinal cord injury mediated by antibodies to neurite growth inhibitors (1995) Nature, 378, pp. 498-501; 
Bradbury, E.J., Moon, L.D., Popat, R.J., Chondroitinase ABC promotes functional recovery after spinal cord injury (2002) Nature, 416, pp. 636-640; 
Bareyre, F.M., Kerschensteiner, M., Raineteau, O., The injured spinal cord spontaneously forms a new intraspinal circuit in adult rats (2004) Nature Neurosci, 7, pp. 269-277; 
Saberi, H., Firouzi, M., Habibi, Z., Safety of intramedullary Schwann cell transplantation for postrehabilitation spinal cord injuries: 2-year follow-up of 33 cases (2011) J Neurosurg Spine, 15, pp. 515-525; 
Fukushima, K., Enomoto, M., Tomizawa, S., The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord (2008) J Med Dent Sci, 55, pp. 71-79; 
Madaghiele, M., Sannino, A., Yannas, I.V., Collagen-based matrices with axially oriented pores (2008) J Biomed Mater Res A, 85, pp. 757-767; 
Liang, W., Han, Q., Jin, W., The promotion of neurological recovery in the rat spinal cord crushed injury model by collagen-binding BDNF (2010) Biomaterials, 31, pp. 8634-8641; 
Liu, T., Houle, J.D., Xu, J., Nanofibrous Collagen Nerve Conduits for Spinal Cord Repair (2012) Tissue Eng Pt A, 18, pp. 1057-1066; 
Khaing, Z.Z., Milman, B.D., Vanscoy, J.E., High molecular weight hyaluronic acid limits astrocyte activation and scar formation after spinal cord injury (2011) J Neural Eng, 8, p. 046033; 
Mehrotra, S., Lynam, D., Maloney, R., Time Controlled Protein Release from Layer-by-Layer Assembled Multilayer Functionalized Agarose Hydrogels (2010) Adv Funct Mater, 20, pp. 247-258; 
Kataoka, K., Suzuki, Y., Kitada, M., Alginate enhances elongation of early regenerating axons in spinal cord of young rats (2004) Tissue Eng, 10, pp. 493-504; 
Chen, X., Yang, Y., Yao, J., Bone marrow stromal cells-loaded chitosan conduits promote repair of complete transection injury in rat spinal cord (2011) J Mater Sci Mater Med, 22, pp. 2347-2356; 
Kim, H., Tator, C.H., Shoichet, M.S., Chitosan implants in the rat spinal cord: biocompatibility and biodegradation (2011) J Biomed Mater Res A, 97, pp. 395-404; 
Ding, C., Guo, X., Cheng, B., Fabrication and radial compressive properties of the biodegradable woven regeneration conduits for peripheral nerve repair (2011) Adv Mat Res, 332-334, pp. 1481-1484; 
Suwantong, O., Waleetorncheepsawat, S., Sanchavanakit, N., In vitro biocompatibility of electrospun poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fiber mats (2007) Int J Biol Macromol, 40, pp. 217-223; 
Pritchard, C.D., Slotkin, J.R., Yu, D., Establishing a model spinal cord injury in the African green monkey for the preclinical evaluation of biodegradable polymer scaffolds seeded with human neural stem cells (2010) J Neurosci Meth, 188, pp. 258-269; 
Pires, L.R., Guarino, V., Barrias, C.C., Tunning poly(trymethylene carbonate-co-epsilon-caprolactone) fibers for nerve regeneration (2011) Histol Histopathol, 26, pp. 251-252; 
Tysseling, V.M., Sahni, V., Pashuck, E.T., Self-assembling peptide amphiphile promotes plasticity of serotonergic fibers following spinal cord injury (2010) J Neurosci Res, 88, pp. 3161-3170
},
	month = {2012///},
	pages = {2564 - 2582},
	abstract = {Spinal cord injury triggers a complex set of events that lead to tissue healing without the restoration of normal function due to the poor regenerative capacity of the spinal cord. Nevertheless, current knowledge about the intrinsic regenerative ability of central nervous system axons, when in a supportive environment, has made the prospect of treating spinal cord injury a reality. Among the range of strategies under investigation, cell-based therapies offer the most promising results, due to the multifactorial roles that these cells can fulfil. However, the best cell source is still a matter of debate, as are clinical issues that include the optimal cell dose as well as the timing and route of administration. In this context, the role of biomaterials is gaining importance. These can not only act as vehicles for the administered cells but also, in the case of chronic lesions, can be used to fill the permanent cyst, thus creating a more favourable and conducive environment for axonal regeneration in addition to serving as local delivery systems of therapeutic agents to improve the regenerative milieu. Some of the candidate molecules for the future are discussed in view of the knowledge derived from studying the mechanisms that facilitate the intrinsic regenerative capacity of central nervous system neurons. The future challenge for the multidisciplinary teams working in the field is to translate the knowledge acquired in basic research into effective combinatorial therapies to be applied in the clinic. {\textcopyright} 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.},
	keywords = {Biomaterials, Cell therapies, Clinical trials, Conditioning lesion, Gene therapy, Growth factors, Spinal cord injury, Stem cells},
	isbn = {15821838 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868098934\&partnerID=40\&md5=adb61a7d32054dc21aa4d733bc31427f},
	author = {P{\^e}go, A.P. and Kubinova, S. and Cizkova, D. and Vanicky, I. and Mar, F.M. and Sousa, M.M. and Sykova, E.}
}
@article {2240,
	title = {Gene delivery into mesenchymal stem cells: A biomimetic approach using rgd nanoclusters based on poly(amidoamine) dendrimers},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {12},
	year = {2011},
	note = {Cited By (since 1996): 10Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm1012647PubMed ID: 21210673Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; CQM-Centro de Qu{\'\i}mica da Madeira, MMRG, Universidade da Madeira, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; firefly luciferase, 61970-00-1; Dendrimers; Oligopeptides; Poly(amidoamine); Polyamines; arginyl-glycyl-aspartic acid, 99896-85-2References: Pierschbacher, M.D., Ruoslahti, E., (1984) Nature, 309, pp. 30-33;Ruoslahti, E., (1996) Annu. Rev. Cell Dev. Biol., 12, pp. 697-715; 
Schaffn, P., Dard, M.M., (2003) Cell. Mol. Life Sci., 60, pp. 119-132; 
Arosio, D., Manzoni, L., Araldi, E.M.V., Caprini, A., Monferini, E., Scolastico, C., (2009) Bioconjugate Chem., 20, pp. 1611-1617; 
Cai, W., Chen, X., (2008) Nat. Protoc., 3, pp. 89-96; 
Cai, W., Wu, Y., Chen, K., Cao, Q., Tice, D.A., Chen, X., (2006) Cancer Res., 66, pp. 9673-9681; 
Gaillard, C., Cellot, G., Li, S., Toma, F.M., Dumortier, H., Spalluto, G., Cacciari, B., Bianco, A., (2009) Adv. Mater., 21, pp. 1-6; 
Green, J.J., Chiu, E., Leshchiner, E.S., Shi, J., Langer, R., Anderson, D.G., (2007) Nano Lett., 7, pp. 874-879; 
Hwang, D.S., Sim, S.B., Cha, H.J., (2007) Biomaterials, 28, pp. 4039-4046; 
Irvine, D.J., Mayes, A.M., Griffith, L.G., (2001) Biomacromolecules, 2, pp. 85-94; 
Kong, H.J., Hsiong, S., Mooney, D.J., (2007) Nano Lett., 7, pp. 161-166; 
Lesniak, W.G., Kariapper, M.S., Nair, B.M., Tan, W., Hutson, A., Balogh, L.P., Khan, M.K., (2007) Bioconjugate Chem., 18, pp. 1148-1154; 
Ng, Q.K., Sutton, M.K., Soonsawad, P., Xing, L., Cheng, H., Segura, T., (2009) Mol. Ther., 17, pp. 828-836; 
Suk, J.S., Suh, J., Choy, K., Lai, S.K., Fu, J., Hanes, J., (2006) Biomaterials, 27, pp. 5143-5150; 
Sun, Y.X., Zeng, X., Meng, Q.F., Zhang, X.Z., Cheng, S.X., Zhuo, R.X., (2008) Biomaterials, 29, pp. 4356-4365; 
Yang, H., Kao, W.J., (2007) Int. J. Nanomed., 2, pp. 89-99; 
Yoshimoto, M., Ogawa, K., Washiyama, K., Shikano, N., Mori, H., Amano, R., Kawai, K., (2008) Int. J. Cancer, 123, pp. 709-715; 
Bidarra, S.J., Barrias, C.C., Barbosa, M.A., Soares, R., Granja, P.L., (2010) Biomacromolecules, 11, pp. 1956-1964; 
Grellier, M., Granja, P.L., Fricain, J.-C., Bidarra, S.J., Renard, M., Bareille, R., Bourget, C., Barbosa, M.A., (2009) Biomaterials, 30, pp. 3271-8; 
Evangelista, M.B., Hsiong, S., Fernandes, R., Sampaio, P., Kong, H.J., Barrias, C.C., Salema, R., Granja, P.L., (2007) Biomaterials, 28, pp. 3644-55; 
Alsberg, E., Anderson, K.W., Albeiruti, A., Franceschi, R.T., Mooney, D.J., (2001) J. Dental Res., 80, pp. 2025-9; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., (1999) Biomaterials, 20, pp. 45-53; 
Hynes, R.O., (2002) Cell, 110, pp. 673-687; 
Qin, J., Vinogradova, O., Plow, E.F., (2004) PLoS Biol., 2, pp. e169; 
Medina-Kauwe, L.K., (2003) Adv. Drug Delivery Rev., 55, pp. 1485-1496; 
Stewart, P.L., Chiu, C.Y., Huang, S., Muir, T., Zhao, Y., Chait, B., Mathias, P., Nemerow, G.R., (1997) EMBO J., 16, pp. 1189-1198; 
Duf{\`e}s, C., Uchegbu, I.F., Sch{\"a}tzlein, A.G., (2005) Adv. Drug Delivery Rev., 57, pp. 2177-2202; 
Mintzer, M.A., Simanek, E.E., (2009) Chem. Rev., 109, pp. 259-302; 
Smith, D.K., (2008) Curr. Top. Med. Chem., 8, pp. 1187-1203; 
Svenson, S., Tomalia, D.A., (2005) Adv. Drug Delivery Rev., 57, pp. 2106-2129; 
Dennig, J., Duncan, E., (2002) J. Biotechnol., 90, pp. 339-347; 
Sokolova, V.V., Radtke, I., Heumann, R., Epple, M., (2006) Biomaterials, 27, pp. 3147-3153; 
Kukowska-Latallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., Baker Jr., J.R., (1996) Proc. Natl. Acad. Sci. U.S.A., 93, pp. 4897-4902; 
Santos, J.L., Oramas, E., P{\^e}go, A.P., Granja, P.L., Tom{\'a}s, H., (2009) J. Controlled Release, 134, pp. 141-148; 
Prockop, D.J., (2009) Mol. Ther., 17, pp. 939-946; 
V{\"a}{\"a}n{\"a}nen, H.K., (2005) Ann. Med., 37, pp. 469-479; 
Gronthos, S., Simmons, P.J., Graves, S.E., Robey, P.G., (2001) Bone, 28, pp. 174-181; 
Snyder, S.L., Sobocinski, P.Z., (1975) Anal. Biochem., 64, pp. 284-288; 
Haensler, J., Szoka Jr., F.C., (1993) Bioconjugate Chem, 4, pp. 372-379; 
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., (1985) Anal. Biochem., 150, pp. 76-85; 
Perrot, S., Dutertre-Catella, H., Martin, C., Rat, P., Warnet, J.M., (2003) Toxicol. Sci., 72, pp. 122-129; 
Hosseinkhani, H., Yamamoto, M., Inatsugu, Y., Hiraoka, Y., Inoue, S., Shimokawa, H., Tabata, Y., (2006) Biomaterials, 27, pp. 1387-1398; 
Zhang, S., Li, J., Lykotrafitis, G., Bao, G., Suresh, S., (2009) Adv. Mater., 21, pp. 419-424; 
Tang, M.X., Szoka, F.C., (1997) Gene Ther., 4, pp. 823-32; 
Santos, J.L., Pandita, D., Rodrigues, J., P{\^e}go, A.P., Granja, P.L., Balian, G., Tom{\'a}s, H., (2010) Mol. Pharm., 7, pp. 763-774; 
Santos, J.L., Oliveira, H., Pandita, D., Rodrigues, J., P{\^e}go, A.P., Granja, P.L., Tom{\'a}s, H., (2010) J. Controlled Release, 144, pp. 55-64; 
Kunath, K., Merdan, T., Hegener, O., H{\"a}berlein, H., Kissel, T., (2003) J. Gene Med., 5, pp. 588-599; 
Lechardeur, D., Verkman, A.S., Lukacs, G.L., (2005) Adv. Drug Delivery Rev., 57, pp. 755-767; 
Ryoo, H.M., Lee, M.H., Kim, Y.J., (2006) Gene, 366, pp. 51-57
},
	month = {2011///},
	pages = {472 - 481},
	abstract = {Poly(amidoamine) dendrimers (generations 5 and 6) with amine termini were conjugated with peptides containing the arginine-glycine-aspartic acid (RGD) sequence having in view their application as gene delivery vectors. The idea behind the work was to take advantage of the cationic nature of dendrimers and of the integrin targeting capabilities of the RGD motif to improve gene delivery. Dendrimers were used as scaffolds for RGD clustering and, by controlling the number of peptides (4, 8, and 16) linked to each dendrimer, it was possible to evaluate the effect of RGD density on the gene delivery process. The new vectors were characterized in respect to their ability to neutralize and compact plasmid DNA (pDNA). The complexes formed by the vectors and pDNA were studied concerning their size, zeta potential, capacity of being internalized by cells and ability of transferring genes. Transfection efficiency was analyzed, first, by using a pDNA encoding for Enhanced Green Fluorescent Protein and Firefly Luciferase and, second, by using a pDNA encoding for Bone Morphogenetic Protein-2. Gene expression in mesenchymal stem cells was enhanced using the new vectors in comparison to native dendrimers and was shown to be dependent on the electrostatic interaction established between the dendrimer moiety and the cell surface, as well as on the RGD density of nanoclusters. The use of dendrimer scaffolds for RGD cluster formation is a new approach that can be extended beyond gene delivery applications, whenever RGD clustering is important for modulating cellular responses. {\textcopyright} 2011 American Chemical Society.},
	keywords = {Amino Acid Sequence, Amino acids, animal cell, animal experiment, Animals, Arginine-glycine-aspartic acids, arginylglycylaspartic acid, article, Biomimetics, bone morphogenetic protein 2, Bone morphogenetic protein-2, Cell culture, Cell membranes, cell surface, Cell surfaces, cell survival, Cellular response, Cluster formations, Complexation, controlled study, dendrimer, Dendrimers, Electrostatic interactions, electrostatic precipitation, Encoding (symbols), Enhanced green fluorescent protein, Firefly luciferase, Functional polymers, Gene delivery, Gene delivery vectors, gene expression, gene targeting, Gene therapy, Gene transfer, Gene Transfer Techniques, gene vector, integrin, Integrins, male, Mesenchymal stem cell, Mesenchymal Stem Cells, Molecular Structure, Nanoclusters, Nanostructures, New approaches, nonhuman, Oligopeptides, Organic acids, Peptides, plasmid DNA, Plasmid DNA (pDNA), polyamidoamine, Polyamidoamine dendrimers, Polyamines, priority journal, rat, Rats, Rats, Wistar, RGD motif, Scaffolds, Stem cells, Structure-Activity Relationship, Targeting capability, Transfection efficiency, Vectors, Zeta potential},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79951658350\&partnerID=40\&md5=a3ef0b57d12de8efb52ba2bf445be126},
	author = {Pandita, D. and Santos, J.L. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2231,
	title = {Molecular recognition force spectroscopy: A new tool to tailor targeted nanoparticles},
	journal = {SmallSmall},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1002/smll.201002074Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis̃o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Verma, I.M., Weitzman, M.D., (2005) Annu. Rev. Biochem., 74, pp. 711-738;Midoux, P., Breuzard, G., Gomez, J.P., Pichon, C., (2008) Curr. Gene Ther., 8, pp. 335-352; 
Oliveira, H., Fernandez, R., Pires, L.R., Martins, M.C., Simoes, S., Barbosa, M.A., Pego, A.P., (2010) J. Controlled Release, 143, pp. 350-358; 
Hinterdorfer, P., Baumgartner, W., Gruber, H.J., Schilcher, K., Schindler, H., (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 3477-3481; 
Ebner, A., Wildling, L., Kamruzzahan, A.S., Rankl, C., Wruss, J., Hahn, C.D., Holzl, M., Gruber, H.J., (2007) Bioconjugate Chem., 18, pp. 1176-1184; 
Fotinou, C., Emsley, P., Black, I., Ando, H., Ishida, H., Kiso, M., Sinha, K.A., Isaacs, N.W., (2001) J. Biol. Chem., 276, pp. 32274-32281; 
Ebner, A., Hinterdorfer, P., Gruber, H.J., (2007) Ultramicroscopy, 107, pp. 922-927; 
Baumgartner, W., Hinterdorfer, P., Schindler, H., (2000) Ultramicroscopy, 82, pp. 85-95; 
Evans, E., Ritchie, K., (1997) Biophys. J., 72, pp. 1541-1555; 
Evans, E., Ritchie, K., (1999) Biophys. J., 76, pp. 2439-2447; 
Bell, G.I., (1978) Science, 200, pp. 618-627; 
Sinha, K., Box, M., Lalli, G., Schiavo, G., Schneider, H., Groves, M., Siligardi, G., Fairweather, N., (2000) Mol. Microbiol., 37, pp. 1041-1051; 
Halpern, J.L., Loftus, A., (1993) J. Biol. Chem., 268, pp. 11188-11192; 
Owens III, D.E., Peppas, N.A., (2006) Int. J. Pharm., 307, pp. 93-102; 
Storm, G., Belliot, S.O., Daemen, T., Lasic, D.D., (1995) Adv. Drug Delivery Rev., 17, pp. 31-48; 
Gu, F., Zhang, L., Teply, B.A., Mann, N., Wang, A., Radovic-Moreno, A.F., Langer, R., Farokhzad, O.C., (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 2586-2591; 
Butt, H.J., Jaschke, M., (1995) Nanotechnology, 6, pp. 1-7; 
Hutter, J.L., Bechhoefer, J., (1993) Rev. Sci. Instrum., 64, pp. 1868-1873; 
Rankl, C., Kienberger, F., Wildling, L., Wruss, J., Gruber, H.J., Blaas, D., Hinterdorfer, P., (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 17778-17783
},
	month = {2011///},
	pages = {1236 - 1241},
	abstract = {The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine. A novel screening tool is described to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. The unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. {\textcopyright} 2011 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {article, Atomic force microscopy, Atomic spectroscopy, atomic-force microscopy, chemistry, Event probability, Force spectroscopy, Functionalized nanoparticles, gene technology, Gene-delivery systems, Genes, Medical nanotechnology, medicine, methodology, Microscopy, Atomic Force, Molecular biology, Molecular recognition, molecular recognition force spectroscopy, Nanomedicines, nanoparticle, Nanoparticle formulation, nanoparticles, Nanotechnology, Neuronal cell, Optimization, Receptor interaction, Screening tool, Specific interaction, Targeted nanoparticle},
	isbn = {16136810 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955561627\&partnerID=40\&md5=85fa523edfcd7a6e171a53820be7f230},
	author = {Oliveira, H. and Rangl, M. and Ebner, A. and Mayer, B. and Hinterdorfer, P. and P{\^e}go, A.P.}
}
@article {2239,
	title = {Non-viral gene delivery to mesenchymal stem cells: Methods, strategies and application in bone tissue engineering and regeneration},
	journal = {Current Gene TherapyCurr. Gene Ther.},
	volume = {11},
	year = {2011},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: CGTUAdoi: 10.2174/156652311794520102PubMed ID: 21182464Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; CQM-Centro de Qu{\'\i}mica da Madeira, MMRG, Universidade da Madeira, Campus Universit{\'a}rio da Penteada, 9000-390 Funchal, Portugal; email: lenat@uma.ptReferences: Bianco, P., Robey, P.G., Simmons, P.J., Mesenchymal stem cells: Revisitinghistory, concepts, and assays (2008) Cell Stem Cell, 2, pp. 313-319;Marot, D., Knezevic, M., Novakovic, G.V., Bone tissue engineeringwith human stem cells (2010) Stem Cell Res Ther, 1, p. 10; 
Caplan, A.I., Review: Mesenchymal stem cells: Cell-based reconstructivetherapy in orthopedics (2005) Tissue Eng, 11, pp. 1198-1211; 
Caplan, A.I., Bruder, S.P., Mesenchymal stem cells: Building blocksfor molecular medicine in the 21st century (2001) Trends Mol Med, 7, pp. 259-264; 
Chen, F.H., Tuan, R.S., Mesenchymal stem cells in arthritic diseases (2008) Arthritis Res Ther, 10, p. 223; 
Da Silva Meirelles, L., Chagastelles, P.C., Nardi, N.B., Mesenchymalstem cells reside in virtually all post-natal organs and tissues (2006) J CellSci, 119, pp. 2204-2213; 
Mauney, J.R., Volloch, V., Kaplan, D.L., Role of adult mesenchymalstem cells in bone tissue engineering applications: Current statusand future prospects (2005) Tissue Eng, 11, pp. 787-802; 
Prockop, D.J., Repair of tissues by adult stem/progenitor cells(MSCs): Controversies, myths, and changing paradigms (2009) Mol Ther, 17, pp. 939-946; 
Sakurada, K., McDonald, F.M., Shimada, F., Regenerative medicineand stem cell based drug discovery (2008) Angew Chem Int Ed Engl, 47, pp. 5718-5738; 
V{\"a}{\"a}n{\"a}nen, H.K., Mesenchymal stem cells (2005) Ann Med, 37, pp. 469-479; 
Jackson, W.M., Nesti, L.J., Tuan, R.S., Potential therapeutic applicationsof muscle-derived mesenchymal stem and progenitor cells (2010) ExpertOpin Biol Ther, 10, pp. 505-517; 
Nauta, A.J., Fibbe, W.E., Immunomodulatory properties of mesenchymalstromal cells (2007) Blood, 110, pp. 3499-3506; 
Heyde, M., Partridge, K.A., Oreffo, R.O., Gene therapy used fortissue engineering applications (2007) J Pharm Pharmacol, 59, pp. 329-350; 
Kimelman, N., Pelled, G., Helm, G.A., Review: Gene-and stemcell-based therapeutics for bone regeneration and repair (2007) TissueEng, 13, pp. 1135-1150; 
Partridge, K.A., Oreffo, R.O., Gene delivery in bone tissue engineering: Progress and prospects using viral and nonviral strategies (2004) TissueEng, 10, pp. 295-307; 
Pittenger, M., Vanguri, P., Simonetti, D., Adult mesenchymalstem cells: Potential for muscle and tendon regeneration and use ingene therapy (2002) J Musculoskelet Neuronal Interact, 2, pp. 309-320; 
Reiser, J., Zhang, X.Y., Hemenway, C.S., Potential of mesenchymalstem cells in gene therapy approaches for inherited and acquireddiseases (2005) Expert Opin Biol Ther, 5, pp. 1571-1584; 
Saraf, A., Mikos, A.G., Gene delivery strategies for cartilage tissueengineering (2006) Adv Drug Deliv Rev, 58, pp. 592-603; 
Trippel, S.B., Ghivizzani, S.C., Nixon, A.J., Gene-based approaches forthe repair of articular cartilage (2004) Gene Ther, 11, pp. 351-359; 
Takahashi, K., Yamanaka, S., Induction of pluripotent stem cells frommouse embryonic and adult fibroblast cultures by defined factors (2006) Cell, 126, pp. 663-676; 
Yu, J., Vodyanik, M.A., Smuga-Otto, K., Induced pluripotentstem cell lines derived from human somatic cells (2007) Science, 318, pp. 1917-1920; 
Steinert, A.F., Noth, U., Tuan, R.S., Concepts in gene therapy for cartilagerepair (2008) Injury, 39 (SUPPL 1), pp. S97-S113; 
Gafni, Y., Turgeman, G., Liebergal, M., Stem cells as vehiclesfor orthopedic gene therapy (2004) Gene Ther, 11, pp. 417-426; 
Kane, N.M., McRae, S., Denning, C., Viral and non-viral genedelivery and its role in pluripotent stem cell engineering Drug DiscovToday: Technol, , In press. doi:10.1016/j.ddtec.2008.10.002; 
Li, S.D., Huang, L., Non-viral is superior to viral gene delivery (2007) JControl Release, 123, pp. 181-183; 
Mastrobattista, E., Bravo, S.A., van der Aa, M., Nonviral genedelivery systems: From simple transfection agents to artificial viruses (2005) Drug Discov Today: Technol, 2, pp. 103-109; 
Chan, J., O{\textquoteright}Donoghue, K., de la Fuente, J., Human fetal mesenchymalstem cells as vehicles for gene delivery (2005) Stem Cells, 23, pp. 93-102; 
Tsuda, H., Wada, T., Yamashita, T., Enhanced osteoinduction bymesenchymal stem cells transfected with a fiber-mutant adenoviralBMP2 gene (2005) J Gene Med, 7, pp. 1322-1334; 
Zhang, X., Godbey, W.T., Viral vectors for gene delivery in tissueengineering (2006) Adv Drug Deliv Rev, 58, pp. 515-534; 
Dang, J.M., Leong, K.W., Natural polymers for gene delivery and tissue engineering (2006) Adv Drug Deliv Rev, 58, pp. 487-499; 
Luten, J., van Nostrum, C.F., De Smedt, S.C., Biodegradablepolymers as non-viral carriers for plasmid DNA delivery (2008) J ControlRelease, 126, pp. 97-110; 
Mintzer, M.A., Simanek, E.E., Nonviral vectors for gene delivery (2009) Chem Rev, 109, pp. 259-302; 
Panyam, J., Labhasetwar, V., Biodegradable nanoparticles for drugand gene delivery to cells and tissue (2003) Adv Drug Deliv Rev, 55, pp. 329-347; 
Choi, J.S., Ko, K.S., Park, J.S., Dexamethasone conjugatedpoly(amidoamine) dendrimer as a gene carrier for efficient nucleartranslocation (2006) Int J Pharm, 320, pp. 171-178; 
Roth, C.M., Sundaram, S., Engineering synthetic vectors for improvedDNA delivery: Insights from intracellular pathways (2004) Annu RevBiomed Eng, 6, pp. 397-426; 
Wood, K.C., Little, S.R., Langer, R., A family of hierarchicallyself-assembling linear-dendritic hybrid polymers for highly efficienttargeted gene delivery (2005) Angew Chem Int Ed Engl, 44, pp. 6704-6708; 
Mehier-Humbert, S., Guy, R.H., Physical methods for gene transfer: Improving the kinetics of gene delivery into cells (2005) Adv Drug DelivRev, 57, pp. 733-753; 
Jo, J., Tabata, Y., Non-viral gene transfection technologies for geneticengineering of stem cells (2008) Eur J Pharm Biopharm, 68, pp. 90-104; 
Song, L., Chau, L., Sakamoto, Y., Electric field-induced molecularvibration for noninvasive, high-efficiency DNA transfection (2004) Mol Ther, 9, pp. 607-616; 
Han, S.W., Nakamura, C., Kotobuki, N., High-efficiency DNAinjection into a single human mesenchymal stem cell using ananoneedle and atomic force microscopy (2008) Nanomedicine, 4, pp. 215-225; 
Park, S., Kim, Y.S., Kim, W.B., Carbon nanosyringe array as aplatform for intracellular delivery (2009) Nano Lett, 9, pp. 1325-1329; 
Durieux, A.C., Bonnefoy, R., Busso, T., In vivo gene electrotransferinto skeletal muscle: Effects of plasmid DNA on the occurrenceand extent of muscle damage (2004) J Gene Med, 6, pp. 809-816; 
Winn, S.R., Hu, Y., Sfeir, C., Gene therapy approaches for modulatingbone regeneration (2000) Adv Drug Deliv Rev, 42, pp. 121-138; 
Lv, H., Zhang, S., Wang, B., Toxicity of cationic lipids and cationicpolymers in gene delivery (2006) J Control Release, 114, pp. 100-109; 
Gebhart, C.L., Kabanov, A.V., Evaluation of polyplexes as gene transferagents (2001) J Control Release, 73, pp. 401-416; 
Hamm, A., Krott, N., Breibach, I., Efficient transfection methodfor primary cells (2002) Tissue Eng, 8, pp. 235-245; 
Gill, D.R., Pringle, I.A., Hyde, S.C., Progress and prospects: The designand production of plasmid vectors (2009) Gene Ther, 16, pp. 165-171; 
Miller, A.M., Dean, D.A., Tissue-specific and transcription factormediatednuclear entry of DNA (2009) Adv Drug Deliv Rev, 61, pp. 603-613; 
Smith, D.K., Dendrimers and the double helix--from DNA bindingtowards gene therapy (2008) Curr Top Med Chem, 8, pp. 1187-1203; 
Duzgunes, N., De Ilarduya, C.T., Simoes, S., Cationic liposomesfor gene delivery: Novel cationic lipids and enhancement by proteinsand peptides (2003) Curr Med Chem, 10, pp. 1213-1220; 
Hoekstra, D., Rejman, J., Wasungu, L., Gene delivery by cationiclipids: In and out of an endosome (2007) Biochem Soc Trans, 35, pp. 68-71; 
Hoelters, J., Ciccarella, M., Drechsel, M., Nonviral genetic modificationmediates effective transgene expression and functionalRNA interference in human mesenchymal stem cells (2005) J Gene Med, 7, pp. 718-728; 
Ko, B.S., Chang, T.C., Shyue, S.K., An efficient transfectionmethod for mouse embryonic stem cells (2009) Gene Ther, 16, pp. 154-158; 
Park, J., Lutz, R., Felszeghy, E., The effect on bone regenerationof a liposomal vector to deliver BMP-2 gene to bone grafts in periimplantbone defects (2007) Biomaterials, 28, pp. 2772-2782; 
Park, J., Ries, J., Gelse, K., Bone regeneration in critical sizedefects by cell-mediated BMP-2 gene transfer: A comparison of adenoviral vectors and liposomes (2003) Gene Ther, 10, pp. 1089-1098; 
Vanderbyl, S., McDonald, G.N., Sidhu, S., Transfer and stabletransgene expression of a mammalian artificial chromosome intobone marrow-derived human mesenchymal stem cells (2004) Stem Cells, 22, pp. 324-333; 
de Jong, G., Telenius, A., Vanderbyl, S., Efficient in-vitro transferof a 60-Mb mammalian artificial chromosome into murine and hamster cells using cationic lipids and dendrimers (2001) ChromosomeRes, 9, pp. 475-485; 
Dass, C.R., Cytotoxicity issues pertinent to lipoplex-mediated genetherapy in-vivo (2002) J Pharm Pharmacol, 54, pp. 593-601; 
Wu, G.Y.W.C., Receptor-mediated in vitro gene transformation by asoluble DNA carrier system (1987) J Biol Chem, 262, pp. 4429-4432; 
Choi, Y.H., Liu, F., Kim, J.S., Polyethylene glycol-grafted poly-Llysineas polymeric gene carrier (1998) J Control Release, 54, pp. 39-48; 
Liu, G., Molas, M., Grossmann, G.A., Biological properties of poly-L-lysine-DNA complexes generated by cooperative binding of the polycation (2001) J Biol Chem, 276, pp. 34379-34387; 
Farrell, L.L., Pepin, J., Kucharski, C., A comparison of the effectivenessof cationic polymers poly-L-lysine (PLL) and polyethylenimine(PEI) for non-viral delivery of plasmid DNA to bone marrowstromal cells (BMSC) (2007) Eur J Pharm Biopharm, 65, pp. 388-397; 
Incani, V., Tunis, E., Clements, B.A., Palmitic acid substitutionon cationic polymers for effective delivery of plasmid DNA tobone marrow stromal cells (2007) J Biomed Mater Res A, 81, pp. 493-504; 
Clements, B.A., Incani, V., Kucharski, C., A comparative evaluationof poly-L-lysine-palmitic acid and Lipofectamine 2000 forplasmid delivery to bone marrow stromal cells (2007) Biomaterials, 28, pp. 4693-4704; 
Bragonzi, A., Boletta, A., Biffi, A., Comparison between cationicpolymers and lipids in mediating systemic gene delivery to thelungs (1999) Gene Ther, 6, pp. 1995-2004; 
Sea, F., Exgen 500 is an efficient vector for gene delivery tolung epithelial cells in vitro and in vivo (1997) Gene Ther, 4, pp. 1100-1106; 
Wightman, L., Kircheis, R., Rossler, V., Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo (2001) J Gene Med, 3, pp. 362-372; 
Behr, J.-P., The Proton Sponge: A Trick to Enter Cells the VirusesDid Not Exploit (1997) Chimia, 51, pp. 34-36; 
Dea, F., A novel non-viral vector for DNA delivery based onlow molecular weight, branched polyethylenimine: Effect of molecularweight on transfection efficiency and cytotoxicity (1999) Pharm. Res, 16, pp. 1273-1279; 
Godbey, W.T., Wu, K.K., Mikos, A.G., Size matters: Molecular weightaffects the efficiency of poly(ethylenimine) as a gene delivery vehicle (1999) J Biomed Mater Res, 45, pp. 268-275; 
Ahn, H.H., Lee, M.S., Cho, M.H., DNA/PEI nano-particles forgene delivery of rat bone marrow stem cells (2008) Colloids and SurfacesA: Physicochem Eng Aspects, 313-314, pp. 116-120; 
Ahn, H.H., Lee, J.H., Kim, K.S., Polyethyleneimine-mediated genedelivery into human adipose derived stem cells (2008) Biomater, 29, pp. 2415-2422; 
Clements, B.A., Bai, J., Kucharski, C., RGD conjugation to polyethyleneiminedoes not improve DNA delivery to bone marrowstromal cells (2006) Biomacromolecules, 7, pp. 1481-1488; 
Saraf, A., Hacker, M.C., Sitharaman, B., Synthesis and conformationalevaluation of a novel gene delivery vector for human mesenchymalstem cells (2008) Biomacromolecules, 9, pp. 818-827; 
Lei, Y., Segura, T., DNA delivery from matrix metalloproteinasedegradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymalstem cells (2009) Biomaterials, 30, pp. 254-265; 
Gwak, S.J., Kim, B.S., Poly(lactic-co-glycolic acid) nanosphere as avehicle for gene delivery to human cord blood-derived mesenchymalstem cells: Comparison with polyethylenimine (2008) Biotechnol Lett, 30, pp. 1177-1182; 
Yang, F., Green, J.J., Dinio, T., Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulatepolymeric vectors (2009) Gene Ther, 16, pp. 533-546; 
Huang, M., Fong, C.W., Khor, E., Transfection efficiency of chitosan vectors: Effect of polymer molecular weight and degree of deacetylation (2005) J Control Release, 106, pp. 391-406; 
Ishii, T., Okahata, Y., Sato, T., Mechanism of cell transfection withplasmid/chitosan complexes (2001) Biochim Biophys Acta, 1514, pp. 51-64; 
Moreira, C., Oliveira, H., Pires, L.R., Improving chitosanmediatedgene transfer by the introduction of intracellular bufferingmoieties into the chitosan backbone (2009) Acta Biomater, 5, pp. 2995-3006; 
Sato, T., Ishii, T., Okahata, Y., In vitro gene delivery mediated bychitosan. effect of pH, serum, and molecular mass of chitosan onthe transfection efficiency (2001) Biomaterials, 22, pp. 2075-2080; 
Corsi, K., Chellat, F., Yahia, L., Mesenchymal stem cells, MG63and HEK293 transfection using chitosan-DNA nanoparticles (2003) Biomaterials, 24, pp. 1255-1264; 
Nie, H., Wang, C.H., Fabrication and characterization of PLGA/HApcomposite scaffolds for delivery of BMP-2 plasmid DNA (2007) J ControlRelease, 120, pp. 111-121; 
Hu, Y., Cai, K., Luo, Z., Surface mediated in situ differentiationof mesenchymal stem cells on gene-functionalized titanium filmsfabricated by layer-by-layer technique (2009) Biomaterials, 30, pp. 3626-3635; 
Okazaki, A., Jo, J., Tabata, Y., A reverse transfection technology togenetically engineer adult stem cells (2007) Tissue Eng, 13, pp. 245-251; 
Hosseinkhani, H., Azzam, T., Kobayashi, H., Combination of 3Dtissue engineered scaffold and non-viral gene carrier enhance in vitroDNA expression of mesenchymal stem cells (2006) Biomaterials, 27, pp. 4269-4278; 
Hosseinkhani, H., Yamamoto, M., Inatsugu, Y., Enhanced ectopicbone formation using a combination of plasmid DNA impregnationinto 3-D scaffold and bioreactor perfusion culture (2006) Biomaterials, 27, pp. 1387-1398; 
Duf{\`e}s, C., Uchegbu, I.F., Sch{\"a}tzlein, A.G., Dendrimers in gene delivery (2005) Adv Drug Deliv Rev, 57, pp. 2177-2202; 
Svenson, S., Tomalia, D.A., Dendrimers in biomedical applications--reflections on the field (2005) Adv Drug Deliv Rev, 57, pp. 2106-2129; 
Malik, N., Wiwattanapatapee, R., Klopsch, R., Dendrimers: Relationshipbetween structure and biocompatibility in vitro, and preliminarystudies on the biodistribution of 125I-labelled polyamidoaminedendrimers in vivo (2000) J Control Release, 65, pp. 133-148; 
Duncan, R., Izzo, L., Dendrimer biocompatibility and toxicity (2005) AdvDrug Deliv Rev, 57, pp. 2215-2237; 
Dennig, J., Duncan, E., Gene transfer into eukaryotic cells usingactivated polyamidoamine dendrimers (2002) J Biotechnol, 90, pp. 339-347; 
Sokolova, V.V., Radtke, I., Heumann, R., Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles (2006) Biomaterials, 27, pp. 3147-3153; 
Santos, J.L., Oramas, E., Pego, A.P., Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene deliveryvectors (2009) J Control Release, 134, pp. 141-148; 
Gheisari, Y., Soleimani, M., Azadmanesh, K., Multipotent mesenchymalstromal cells: Optimization and comparison of five cationicpolymer-based gene delivery methods (2008) Cytotherapy, 10, pp. 815-823; 
Holladay, C., Keeney, M., Greiser, U., A matrix reservoir forimproved control of non-viral gene delivery (2009) J Control Release, 136, pp. 220-225; 
Parekh, H.S., The advance of dendrimers-a versatile targeting platformfor gene/drug delivery (2007) Curr Pharm Des, 13, pp. 2837-2850; 
Santos, J.L., Pandita, D., Rodrigues, J., Receptor-mediated genedelivery using PAMAM dendrimers conjudated with peptides recognizedby mesenchymal stem cells (2010) Mol Pharmaceutics, 7, pp. 763-774; 
Santos, J.L., Oliveira, H., Pandita, D., Rodrigues, J., Functionalizationof Poly(amidoamine) Dendrimers with hydrophobic chains forimproved gene delivery in mesenchymal stem cells (2010) J Control Release, 144, pp. 55-64; 
Bourgeat-Lami, E., Organic-inorganic nanostructured colloids (2002) JNanosci Nanotechnol, 2, pp. 1-24; 
Chowdhury, E.H., Akaike, T., Bio-functional inorganic materials: Anattractive branch of gene-based nano-medicine delivery for 21stcentury (2005) Curr Gene Ther, 5, pp. 669-676; 
Sokolova, V., Epple, M., Inorganic nanoparticles as carriers of nucleicacids into cells (2008) Angew Chem Int Ed Engl, 47, pp. 1382-1395; 
Uchimura, E., Yamada, S., Uebersax, L., Method for reversetransfection using gold colloid as a nano-scaffold (2007) J Biosci Bioeng, 103, pp. 101-103; 
Gonzalez-McQuire, R., Green, D.W., Partridge, K.A., Coating of human mesenchymal cells in 3D culture with bioinorganicnanoparticles promotes osteoblastic differentiation and gene transfection (2007) Adv Mater, 19, pp. 2236-2240; 
Park, J.S., Na, K., Woo, D.G., Non-viral gene delivery of DNApolyplexed with nanoparticles transfected into human mesenchymalstem cells (2010) Biomaterials, 31, pp. 124-132; 
Peister, A., Mellad, J.A., Wang, M., Stable transfection of MSCsby electroporation (2004) Gene Ther, 11, pp. 224-228; 
Ferreira, E., Potier, E., Logeart-Avramoglou, D., Optimization of a gene electrotransfer method for mesenchymal stem cell transfection (2008) Gene Ther, 15, pp. 537-544; 
Ziv, R., Steinhardt, Y., Pelled, G., Micro-electroporation of mesenchymalstem cells with alternating electrical current pulses (2009) BiomedMicrodevices, 11, pp. 95-101; 
Lim, J.Y., Park, S.H., Jeong, C.H., Microporation is a valuabletransfection method for efficient gene delivery into human umbilicalcord blood-derived mesenchymal stem cells (2010) BMC Biotechnol, 10, p. 38; 
Lee, S.-J., Kang, S.-W., Do, H.-J., Enhancement of bone regenerationby gene delivery of BMP2/Runx2 bicistronic vector into adipose-derived stromal cells (2010) Biomater, 31, pp. 5652-5659; 
Lakshmipathy, U., Pelacho, B., Sudo, K., Efficient transfection of embryonic and adult stem cells (2004) Stem Cells, 22, pp. 531-543; 
Aluigi, M., Fogli, M., Curti, A., Nucleofection is an efficientnonviral transfection technique for human bone marrow-derivedmesenchymal stem cells (2006) Stem Cells, 24, pp. 454-461; 
Nakashima, S., Matsuyama, Y., Nitta, A., Highly efficient transfectionof human marrow stromal cells by nucleofection (2005) TransplantProc, 37, pp. 2290-2292; 
Miller, D.L., Pislaru, S.V., Greenleaf, J.E., Sonoporation: MechanicalDNA delivery by ultrasonic cavitation (2002) Somat Cell Mol Genet, 27, pp. 115-134; 
Otani, K., Yamahara, K., Ohnishi, S., Nonviral delivery of siRNA into mesenchymal stem cells by a combination of ultrasoundand microbubbles (2009) J Control Release, 133, pp. 146-153; 
Aslan, H., Sheyn, D., Gazit, D., Genetically engineered mesenchymalstem cells: Applications in spine therapy (2009) Regen Med, 4, pp. 99-108; 
Chiang, G.G., Rubin, H.L., Cherington, V., Bone marrow stromalcell-mediated gene therapy for hemophilia A: In vitro expression of human factor VIII with high biological activity requires the inclusionof the proteolytic site at amino acid 1648 (1999) Hum Gene Ther, 10, pp. 61-76; 
Gelse, K., von der Mark, K., Schneider, H., Cartilage regeneration bygene therapy (2003) Curr Gene Ther, 3, pp. 305-317; 
Moutsatsos, I.K., Turgeman, G., Zhou, S., Exogenously regulatedstem cell-mediated gene therapy for bone regeneration (2001) Mol Ther, 3, pp. 449-461; 
Phillips, M.I., Tang, Y.L., Genetic modification of stem cells for transplantation (2008) Adv Drug Deliv Rev, 60, pp. 160-172; 
Schwarz, E.J., Reger, R.L., Alexander, G.M., Rat marrow stromalcells rapidly transduced with a self-inactivating retrovirus synthesizeL-DOPA in vitro (2001) Gene Ther, 8, pp. 1214-1223; 
Sun, M., Kong, L., Wang, X., Coexpression of tyrosine hydroxylase, GTP cyclohydrolase I, aromatic amino acid decarboxylase, and vesicular monoamine transporter 2 from a helper virus-freeherpes simplex virus type 1 vector supports high-level, long-termbiochemical and behavioral correction of a rat model of Parkinson{\textquoteright}sdisease (2004) Hum Gene Ther, 15, pp. 1177-1196; 
Turgeman, G., Pittman, D.D., Muller, R., Engineered humanmesenchymal stem cells: A novel platform for skeletal cell mediatedgene therapy (2001) J Gene Med, 3, pp. 240-251; 
Tuszynski, M.H., Thal, L., Pay, M., A phase 1 clinical trial of nerve growth factor gene therapy for Alzheimer disease (2005) Nat Med, 11, pp. 551-555; 
Fang, J., Zhu, Y.Y., Smiley, E., Stimulation of new bone formationby direct transfer of osteogenic plasmid genes (1996) Proc Natl AcadSci U S A, 93, pp. 5753-5758; 
Bonadio, J., Smiley, E., Patil, P., Localized, direct plasmid genedelivery in vivo: Prolonged therapy results in reproducible tissueregeneration (1999) Nat Med, 5, pp. 753-759; 
Kasper, F.K., Young, S., Tanahashi, K., Evaluation of bone regenerationby DNA release from composites of oligo(poly(ethyleneglycol) fumarate) and cationized gelatin microspheres in a criticalsizedcalvarial defect (2006) J Biomed Mater Res A, 78, pp. 335-342; 
Kuroda, S., Kondo, H., Ohya, K., A new technique with calciumphosphate precipitate enhances efficiency of in vivo plasmid DNAgene transfer (2005) J Pharmacol Sci, 97, pp. 227-233; 
Endo, M., Kuroda, S., Kondo, H., Bone regeneration by modifiedgene-activated matrix: Effectiveness in segmental tibial defects inrats (2006) Tissue Eng, 12, pp. 489-497; 
Huang, Y.C., Simmons, C., Kaigler, D., Bone regeneration in a ratcranial defect with delivery of PEI-condensed plasmid DNA encodingfor bone morphogenetic protein-4 (BMP-4) (2005) Gene Ther, 12, pp. 418-426; 
Nie, H., Ho, M.L., Wang, C.K., BMP-2 plasmid loadedPLGA/HAp composite scaffolds for treatment of bone defects innude mice (2009) Biomaterials, 30, pp. 892-901; 
Ono, I., Yamashita, T., Jin, H.Y., Combination of porous hydroxyapatiteand cationic liposomes as a vector for BMP-2 genetherapy (2004) Biomaterials, 25, pp. 4709-4718; 
Kishimoto, K.N., Watanabe, Y., Nakamura, H., Ectopic boneformation by electroporatic transfer of bone morphogenetic protein-4 gene (2002) Bone, 31, pp. 340-347; 
Sheyn, D., Kimelman-Bleich, N., Pelled, G., Ultrasound-basednonviral gene delivery induces bone formation in vivo (2008) Gene Ther, 15, pp. 257-266; 
Hosseinkhani, H., Inatsugu, Y., Hiraoka, Y., Impregnation of plasmid DNA into three-dimensional scaffolds and medium perfusionenhance in vitro DNA expression of mesenchymal stem cells (2005) Tissue Eng, 11, pp. 1459-1475; 
Hosseinkhani, H., Hosseinkhani, M., Gabrielson, N.P., DNAnanoparticles encapsulated in 3D tissue-engineered scaffolds enhanceosteogenic differentiation of mesenchymal stem cells (2007) JBiomed Mater Res A, 85, pp. 47-60; 
Huang, Y.C., Kaigler, D., Rice, K.G., Combined angiogenic and osteogenic factor delivery enhances bone marrow stromal celldrivenbone regeneration (2005) J Bone Miner Res, 20, pp. 848-857; 
Blum, J.S., Barry, M.A., Mikos, A.G., In vivo evaluation of genetherapy vectors in ex vivo-derived marrow stromal cells for boneregeneration in a rat critical-size calvarial defect model (2003) Hum GeneTher, 14, pp. 1689-1701; 
Aslan, H., Zilberman, Y., Arbeli, V., Nucleofection-based ex vivononviral gene delivery to human stem cells as a platform for tissueregeneration (2006) Tissue Eng, 12, pp. 877-889; 
Noel, D., Gazit, D., Bouquet, C., Short-term BMP-2 expression issufficient for in vivo osteochondral differentiation of mesenchymalstem cells (2004) Stem Cells, 22, pp. 74-85
},
	month = {2011///},
	pages = {46 - 57},
	abstract = {Mesenchymal stem cells (MSCs) can be isolated from several tissues in the body, have the ability to selfrenewal, show immune suppressive properties and are multipotent, being able to generate various cell types. At present, due to their intrinsic characteristics, MSCs are considered very promising in the area of tissue engineering and regenerative medicine. In this context, genetic modification can be a powerful tool to control the behavior and fate of these cells and be used in the design of new cellular therapies. Viral systems are very effective in the introduction of exogenous genes inside MSCs. However, the risks associated with their use are leading to an increasing search for non-viral approaches to attain the same purpose, even if MSCs have been shown to be more difficult to transfect in this way. In the past few years, progress was made in the development of chemical and physical methods for non-viral gene delivery. Herein, an overview of the application of those methods specifically to MSCs is given and their use in tissue engineering and regenerative medicine therapeutic strategies highlighted using the example of bone tissue. Key issues and future directions in non-viral gene delivery to MSCs are also critically addressed. {\textcopyright} 2011 Bentham Science Publishers Ltd.},
	keywords = {Animals, article, artificial chromosome, Bone and Bones, Bone Regeneration, bone tissue, Bone tissue engineering, cell based gene therapy, dendrimer, ex vivo gene transfer, Gene delivery, gene targeting, Gene therapy, Gene Transfer Techniques, gene vector, genetic transfection, human, Humans, in vivo gene transfer, liposome, Mesenchymal stem cell, Mesenchymal Stem Cells, nanomedicine, nanoparticle, Non-viral vectors, nonhuman, nonviral gene delivery system, polymer, protein expression, Regeneration, Regenerative Medicine, Tissue engineering, Tissue regeneration},
	isbn = {15665232 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79951686783\&partnerID=40\&md5=c9cc095fac56b04808553b19fd1f5f48},
	author = {Santos, J.L. and Pandita, D. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2227,
	title = {Scientists perception of ethical issues in nanomedicine: A case study},
	journal = {NanomedicineNanomedicine},
	volume = {6},
	year = {2011},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.2217/nnm.11.9PubMed ID: 21506689Language of Original Document: EnglishCorrespondence Address: Silva Costa, H.; IBMC - Instituto de Biologia Molecular e Celular, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: helena.costa@kit.eduReferences: (2006) Nanotechnology for Health, , European Commission European Technology Platform on Nanomedicine Strategic Research Agenda, Brussels, Belgium;(2005) Nanomedicine: An ESF - European Medical Research Councils (EMRC) Forward Look Report, , European Science Foundation ISBN 2-912049-52-0. IREG Strasbourg France; 
Bawa, R., Johnson, S., The ethical dimensions of nanomedicine (2007) Medical Clinics of North America, 91 (5), pp. 881-887. , DOI 10.1016/j.mcna.2007.05.007, PII S0025712507000703; 
Resnik, D.B., Tinkle, S.S., Ethics in nanomedicine (2007) Nanomedicine, 2 (3), pp. 345-350. , http://www.futuremedicine.com/doi/pdf/10.2217/17435889.2.3.345, DOI 10.2217/17435889.2.3.345; 
Ebeling, M.F.E., Mediating uncertainty. Communicating the financial risks of nanotechnologies (2008) Science Communication, 29, pp. 335-361; 
Rogers-Hayden, T., Pidgeon, N., Moving engagement "upstream"? nanotechnologies and the Royal Society and Royal Academy of Engineerings inquiry (2007) Public Underst. Sci., 16, pp. 345-364; 
Petersen, A., Anderson, A., A question of balance or blind faith? scientists and policymakers representations of the benefits and risks of nanotechnologies (2007) NanoEthics, 1, pp. 243-256; 
Martin, B., Richards, E., Scientific knowledge, controversy, and public decision-making (1995) Handbook of Science and Technology Studies, pp. 506-526. , Jasanoff S, Markle GE, Petersen JC, Pinch T (Eds). SAGE, CA, USA; 
Sturgis, P., Allum, N., Science in society: Re-evaluating the deficit model of public attitudes (2004) Public Understanding of Science, 13 (1), pp. 55-74. , DOI 10.1177/0963662504042690; 
Hsieh, H.-F., Shannon, S.E., Three approaches to qualitative content analysis (2005) Qualitative Health Research, 15 (9), pp. 1277-1288. , DOI 10.1177/1049732305276687; 
Patton, M.Q., (2002) Qualitative Research and Evaluation Methods, , SAGE Publications, CA, USA; 
Vala, J., A an{\'a}lise de conte{\'u}do (1986) Metodologia das Ci{\^e}ncias Sociais, pp. 102-128. , Silva AS, Pinto JM (Eds). Porto, Portugal; 
Krippendorff, K., (2004) Content Analysis: An Introduction to Its Methodology, , (Second Edition). SAGE, CA, USA; 
Ghiglione, R., Matalon, B., (1998) Les Enqu{\^e}tes Sociologiques: Th{\'e}ories et Pratiques, , Armand Colin Paris France; 
Allhoff, F., The coming era of nanomedicine (2009) Am. J. Bioeth., 9, pp. 3-11; 
Bawa, R., Johnson, S., Emerging issues in nanomedicine and ethics (2008) Nanotechnology and Society: Current and Emerging Ethical Issues, pp. 207-223. , Allhoff F, Lin P (Eds). Springer Netherlands; 
Sandler, R., Nanomedicine and nanomedical ethics (2009) Am. J. Bioeth., 9, pp. 16-17; 
Ebbesen, M., Jensen, T.G., Nanomedicine: Techniques, potentials, and ethical implications (2006) J. Biomed. Biotechnol., 2006, p. 51516; 
Powell, M.C., New risk or old risk, high risk or no risk? How scientists{\textquoteright} standpoints shape their nanotechnology risk frames (2007) Health, Risk and Society, 9 (2), pp. 173-190. , DOI 10.1080/13698570701306872, PII 778583324; 
Bueno, O., The Drexler-Smalley debate on nanotechnology: Incommensurability at work? (2004) Nanotechnology Challenges: Implications for Philosophy, Ethics and Society, pp. 29-48. , Schummer J, Baird D (Eds). World Scientific Publishing, CA, USA; 
McGinn, R., Ethics and nanotechnology: Views of nanotechnology researchers (2008) NanoEthics, 2, pp. 101-131; 
Wienroth, M., Disciplinarity and research identity in nanoscale science and technologies (2009) Size Matters. Nanobiotechnology and Nano-Medicine: Ethical, Legal and Social Aspects, pp. 157-177. , Ach JS, Weidemann C (Eds). Lit, M{\"u}nster, Germany; 
Leary, S.P., Liu, C.Y., Apuzzo, M.L.J., Toward the emergence of nanoneurosurgery: Part III - Nanomedicine: Targeted nanotherapy, nanosurgery, and progress toward the realization of nanoneurosurgery (2006) Neurosurgery, 58 (6), pp. 1009-1025. , DOI 10.1227/01.NEU.0000217016.79256.16, PII 0000612320060600000001; 
Sullivan, D.C., Ferrari, M., Nanotechnology and tumor imaging: Seizing an opportunity (2004) Molecular Imaging, 3 (4), pp. 364-369. , DOI 10.1162/1535350042973526; 
Pilarski, L.M., Mehta, M.D., Caulfield, T., Kvis, K., Backhouse, C.F., Microsystems and nanoscience for biomedical applications: A view to the future (2006) Nanotechnology: Risk, Ethics and Law, pp. 35-42. , Hunt G, Mehta M (Eds). Earthscan, London, UK; 
Wagner, V., Dullaart, A., Bock, A.-K., Zweck, A., The emerging nanomedicine landscape (2006) Nature Biotechnology, 24 (10), pp. 1211-1217. , DOI 10.1038/nbt1006-1211, PII NBT10061211; 
Sheremeta, L., Nanotechnologies and the ethical conduct of research involving human subjects (2006) Nanotechnology: Risk, Ethics and Law, pp. 247-258. , Hunt G, Mehta M (Eds). Earthscan, London, UK; 
Foladori, G., Invernizzi, N., Z{\'a}yago, E., Two dimensions of the ethical problems related to nanotechnology (2009) NanoEthics, 3, pp. 121-127; 
Godman, M., But is it unique to nanotechnology? (2008) Sci. Eng. Ethics, 14, pp. 391-403; 
Kuiken, T., Nanomedicine and ethics: Is there anything new or unique? (2011) Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 3 (2), pp. 111-118; 
Wood, S., Geldart, A., Jones, R., Crystallizing the nanotechnology debate (2008) Tech. Analys. Strategic Management, 20, pp. 13-27; 
Nordmann, A., If and then: A critique of speculative nanoethics (2007) NanoEthics, 1, pp. 31-46; 
Grunwald, A., Nanotechnology - A new field of ethical inquiry? (2005) Science and Engineering Ethics, 11 (2), pp. 187-201; 
Grunwald, A., From speculative nanoethics to explorative philosophy of nanotechnology (2010) NanoEthics, 4 (2), pp. 91-101; 
www.ibmc.up.pt, Institute for Molecular and Cell Biologywww.ineb.up.pt, Institute for Biomedical Engineeringwww.ipatimup.pt, Institute for Molecular Pathology and Immunologywww.portugalnano.net, Portuguese Nanotechnology Network(2008) Awareness of and Attitudes Toward Nanotechnology and Synthetic Biology: A Report of Findings Based on A National Survey among Adults, , www.nanotechproject.org/process/assets/files/8286/nano_synbio.pdf, Hart Research Associates Inc. Conducted on Behalf of the Project on Emerging Nanotechnologies, The Woodrow Wilson International Center for Scholars; 
McGinn, R., (2008) Nanotechnology and Ethics: A Short Guide to Ethical Responsibilities of Nanotechnology Researchers at NNIN Laboratories, National Nanotechnology Infrastructure Network, , www.sei.nnin.org/doc/nanotechnology_and_ethics_guide.pdf
},
	month = {2011///},
	pages = {681 - 691},
	abstract = {Aim: Research and development in nanomedicine has been accompanied by the consideration of ethical issues; however, little is known about how researchers working in this area perceive such issues. This case-study explores scientists attitude towards and knowledge of ethical issues. Method: Data were collected by semi-structured interviews with 22 nanomedicine practitioners and subject to content analysis. Results: We found that scientists reflect with ambiguity on the reputed novelty of nanomedicine and what the ethical issues and risks are in their work. Respondents see no necessity for a paradigm shift in ethical considerations, but view ethical issues in nanomedicine as overlapping with those of other areas of biomedical research. Most respondents discuss ethical issues they faced in scientific work with their colleagues, but expect benefit from additional information and training on ethics. Conclusion: Our findings that scientists are motivated to reflect on ethical issues in their work, can contribute to the design of new strategies, including training programs, to engage scientists in ethical discussion and stimulate their responsibility as nanomedicine practitioners. {\textcopyright} 2011 Future Medicine Ltd.},
	keywords = {adult, ambiguity, article, attitude, case study, clinical article, empirical research, ethics, Female, human, Humans, information, knowledge, male, medical ethics, medical research, Middle Aged, Models, Theoretical, nanomedicine, Nanotechnology, perception, priority journal, professional practice, qualitative study, Research Personnel, responsibility, risks, scientist, scientists perception, semi structured interview, training, Young Adult},
	isbn = {17435889 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79959957262\&partnerID=40\&md5=33a048f12514a5e04810a0c7d7e311dd},
	author = {Silva Costa, H. and Sethe, S. and P{\^e}go, A.P. and Olsson, I.A.S.}
}
@article {2244,
	title = {Chitosan-based gene delivery vectors targeted to the peripheral nervous system},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32874PubMed ID: 20734332Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; chitosan, 9012-76-4; imidazole, 1467-16-9, 288-32-4; macrogol, 25322-68-3; Cations; Chitosan, 9012-76-4; DNA, 9007-49-2; Polyethylene GlycolsTradenames: Chimarin, medicarb, SwedenManufacturers: medicarb, SwedenReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov Today, 1, pp. 177-185;Apfel, S.C., Neurotrophic factors in peripheral neuropathies: Therapeutic implications (1999) Brain Pathol, 9, pp. 393-413; 
Apfel, S.C., Is the therapeutic application of neurotrophic factors dead? (2002) Ann Neurol, 51, pp. 8-11; 
Glorioso, J.C., Mata, M., Fink, D.J., Therapeutic gene transfer to the nervous system using viral vectors (2003) J Neurovirol, 9, pp. 165-172; 
Fishman, P.S., Carrigan, D.R., Retrograde transneuronal transfer of the C-fragment of tetanus toxin (1987) Brain Res, 406, pp. 275-279; 
Evinger, C., Erichsen, J.T., Transsynaptic retrograde transport of fragment C of tetanus toxin demonstrated by immunohistochemical localization (1986) Brain Res, 380, pp. 383-388; 
Figueiredo, D.M., Hallewell, R.A., Chen, L.L., Fairweather, N.F., Dougan, G., Savitt, J.M., Parks, D.A., Fishman, P.S., Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport (1997) Exp Neurol, 145 (2 PART 1), pp. 546-554; 
Fishman, P.S., Savitt, J.M., Farrand, D.A., Enhanced CNS uptake of systemically administered proteins through conjugation with tetanus C-fragment (1990) J Neurol Sci, 98, pp. 311-325; 
Coen, L., Osta, R., Maury, M., Brulet, P., Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system (1997) Proc Natl Acad Sci USA, 94, pp. 9400-9405; 
Knight, A., Carvajal, J., Schneider, H., Coutelle, C., Chamberlain, S., Fairweather, N., Non-viral neuronal gene delivery mediated by the HC fragment of tetanus toxin (1999) Eur J Biochem, 259, pp. 762-769; 
Oliveira, H., Fernandez, R., Pires, L.R., Sim{\~o}es, S., Martins, M.C.L., Barbosa, M.A., P{\^e}go, A.P., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethilenimine) and tetanus toxin fragment c (2010) J Control Release, 143, pp. 350-358; 
Neu, M., Fischer, D., Kissel, T., Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives (2005) J Gene Med, 7, pp. 992-1009; 
Tang, G.P., Guo, H.Y., Alexis, F., Wang, X., Zeng, S., Lim, T.M., Ding, J., Wang, S., Low molecular weight polyethylenimines linked by beta-cyclodextrin for gene transfer into the nervous system (2006) J Gene Med, 8, pp. 736-744; 
Chollet, P., Favrot, M.C., Hurbin, A., Coll, J.L., Side-effects of a systemic injection of linear polyethylenimine-DNA complexes (2002) J Gene Med, 4, pp. 84-91; 
Shi, C., Zhu, Y., Ran, X., Wang, M., Su, Y., Cheng, T., Therapeutic potential of chitosan and its derivatives in regenerative medicine (2006) J Surg Res, 133, pp. 185-192; 
Mansouri, S., Lavigne, P., Corsi, K., Benderdour, M., Beaumont, E., Fernandes, J.C., Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: Strategies to improve transfection efficacy (2004) Eur J Pharm Biopharm, 57, pp. 1-8; 
Moreira, C., Oliveira, H., Pires, L.R., Simoes, S., Barbosa, M.A., Pego, A.P., Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone (2009) Acta Biomater, 5, pp. 2995-3006; 
Verma, A., Stellacci, F., Effect of surface properties on nanoparticle-cell interactions (2010) Small, 6, pp. 12-21; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
(1987) Guideline on Validation of the Limulus Amebocyte Lysate Test As An End-product Endotoxin Test for Human and Animal Parental Drugs, Biological Products, and Medical Devices, , US Department of Health and Human Services. US Department of Health and Human Services; 
MacUlotti, K., Genta, I., Perugini, P., Imam, M., Bernkop-Schnurch, A., Pavanetto, F., Preparation and in vitro evaluation of thiolated chitosan microparticles (2005) J Microencapsul, 22, pp. 459-470; 
Mao, H., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., August, J.T., Leong, K.W., Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency (2001) J Control Release, 70, pp. 399-421; 
Innes, N.P.T., Ogden, G.R., A technique for the study of endocytosis in human oral epithelial cells (1999) Arch Oral Biol, 44, pp. 519-523; 
O{\textquoteright}Brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) Eur J Biochem, 267, pp. 5421-5426; 
Ellman, G.L., Tissue sulfhydryl groups (1959) Arch Biochem Biophys, 82, pp. 70-77; 
Lee, D., Zhang, W., Shirley, S.A., Kong, X., Hellermann, G.R., Lockey, R.F., Mohapatra, S.S., Thiolated chitosan/DNA nanocomplexes exhibit enhanced and sustained gene delivery (2007) Pharm Res, 24, pp. 157-167; 
Loretz, B., Thaler, M., Bernkop-Schnurch, A., Role of sulfhydryl groups in transfection? A case study with chitosan-NAC nanoparticles (2007) Bioconjug Chem, 18, pp. 1028-1035; 
Deinhardt, K., Berninghausen, O., Willison, H.J., Hopkins, C.R., Schiavo, G., Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin 1 (2006) J Cell Biol, 174, pp. 459-471; 
Williamson, L.C., Neale, E.A., Bafilomycin A1 inhibits the action of tetanus toxin in spinal-cord neurons in cell-culture (1994) J Neurochem, 63, pp. 2342-2345; 
Bohnert, S., Schiavo, G., Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation (2005) J Biol Chem, 280, pp. 42336-42344
},
	month = {2010///},
	pages = {801 - 810},
	abstract = {A non-toxic, targeted, simple and efficient system that can specifically transfect peripheral sensorial neurons can pave the way towards the development of new therapeutics for the treatment of peripheral neuropathies. In this study chitosan (CH), a biodegradable polymer, was used as the starting material in the design of a multicomponent vector targeted to the peripheral nervous system (PNS). Polycation-DNA complexes were optimized using imidazole-and thiol-grafted CH (CHimiSH), in order to increase transfection efficiency and allow the formation of ligand conjugated nanocomplexes, respectively. The 50 kDa non-toxic fragment from the tetanus toxin (HC), shown to interact specifically with peripheral neurons and undergo retrograde transport, was grafted to the binary complex via a bi-functional poly(ethylene glycol) (HC-PEG) reactive for the thiol moieties present in the complex surface. The targeting of the developed nanocomplexes was assessed by means of internalization and transfection studies in the ND7/23 (neuronal) vs. NIH 3T3 (fibroblast) cell lines. Targeted transfection was further confirmed in dorsal root ganglion dissociated primary cultures. A versatile, multi-component nanoparticle system that successfully targets and transfects neuronal cell lines, as well as dorsal root ganglia (DRG) primary neuron cultures was obtained for the 1.0 (w/w) HC-PEG/DNA formulation. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {animal cell, Animals, article, Binary complexes, Biodegradable polymers, Carbohydrate Conformation, Carbohydrate Sequence, Cations, Cell culture, Cell lines, cell population, Cells, Cultured, Chitin, chitosan, Complex surface, DNA, DNA complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Ethylene Glycol, Female, Ganglia, Spinal, Gene delivery, gene delivery system, Gene delivery vectors, Gene therapy, Gene transfer, Gene Transfer Techniques, genetic transfection, Genetic Vectors, Grafting (chemical), Humans, imidazole, macrogol, materials testing, Mice, Molecular Sequence Data, Molecular Structure, mouse, Multicomponents, Nanocomplexes, Nanoparticle systems, nanoparticles, nerve cell culture, Neuron culture, Neuronal cell, NIH 3T3 Cells, nonhuman, Nontoxic fragment of tetanus toxin, Peripheral nervous system, Peripheral neuropathy, Polycations, Polyethylene Glycols, Polyethylene oxides, Pregnancy, Primary culture, rat, Rats, Rats, Wistar, spinal ganglion, Starting materials, Targeted, Tetanus toxin, Toxic materials, Transfection, Transfection efficiency},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78249240032\&partnerID=40\&md5=af9c3ca5ca712d53abb5e80f45ed09c0},
	author = {Oliveira, H. and Pires, L.R. and Fernandez, R. and Martins, M.C.L. and Sim{\~o}es, S. and P{\^e}go, A.P.}
}
@article {2250,
	title = {Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth},
	journal = {Nano LettersNano Lett.},
	volume = {10},
	year = {2010},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: Scopusdoi: 10.1021/nl1016909Language of Original Document: EnglishCorrespondence Address: Schlosshauer, B.; NMI Natural and Medical Sciences Institute, University of T{\"u}bingen, Markwiesenstrasse 55, 72770 Reutlingen, Germany; email: schlosshauer@nmi.deChemicals/CAS: chitosan, 9012-76-4; Chitosan, 9012-76-4; RNA, Small Interfering; rhoA GTP-Binding Protein, 3.6.5.2References: Terzis, J.K., Skoulis, T.G., Soucacos, P.N., (1995) Int. Angiol., 14, pp. 264-77;Schmidt, C.E., Leach, J.B., (2003) Annu. Rev. Biomed. Eng., 5, pp. 293-347; 
Stokols, S., Tuszynski, M.H., (2004) Biomaterials, 25 (27), pp. 5839-46; 
Goraltchouk, A., Scanga, V., Morshead, C.M., Shoichet, M.S., (2006) J. Controlled Release, 110 (2), pp. 400-7; 
Wu, J., Chiu, D.T., (1999) Ann. Plast. Surg., 43, pp. 661-667; 
Chen, M.S., Huber, A.B., Van Der Haar, M.E., Frank, M., Schnell, L., Spillmann, A.A., Christ, F., Schwab, M.E., (2000) Nature, 403 (6768), pp. 434-9; 
Grandpre, T., Nakamura, F., Vartanian, T., Strittmatter, S.M., (2000) Nature, 403 (6768), pp. 439-44; 
Liu, B.P., Fournier, A., Grandpre, T., Strittmatter, S.M., (2002) Science, 297 (5584), pp. 1190-3; 
McKerracher, L., David, S., Jackson, D.L., Kottis, V., Dunn, R.J., Braun, P.E., (1994) Neuron, 13 (4), pp. 805-11; 
Mukhopadhyay, G., Doherty, P., Walsh, F.S., Crocker, P.R., Filbin, M.T., (1994) Neuron, 13 (3), pp. 757-67; 
Wang, K.C., Koprivica, V., Kim, J.A., Sivasankaran, R., Guo, Y., Neve, R.L., He, Z., (2002) Nature, 417 (6892), pp. 941-4; 
Mi, S., Lee, X., Shao, Z., Thill, G., Ji, B., Relton, J., Levesque, M., Pepinsky, R.B., (2004) Nat. Neurosci., 7 (3), pp. 221-8; 
Park, J.B., Yiu, G., Kaneko, S., Wang, J., Chang, J., He, X.L., Garcia, K.C., He, Z., (2005) Neuron, 45 (3), pp. 345-51; 
Shao, Z., Browning, J.L., Lee, X., Scott, M.L., Shulga-Morskaya, S., Allaire, N., Thill, G., Mi, S., (2005) Neuron, 45 (3), pp. 353-9; 
Wang, K.C., Kim, J.A., Sivasankaran, R., Segal, R., He, Z., (2002) Nature, 420 (6911), pp. 74-8; 
Wong, S.T., Henley, J.R., Kanning, K.C., Huang, K.H., Bothwell, M., Poo, M.M., (2002) Nat. Neurosci., 5 (12), pp. 1302-8; 
Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., Tuschl, T., (2001) Nature, 411 (6836), pp. 494-8; 
Higuchi, H., Yamashita, T., Yoshikawa, H., Tohyama, M., (2003) Biochem. Biophys. Res. Commun., 301 (3), pp. 804-9; 
Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., Mello, C.C., (1998) Nature, 391 (6669), pp. 806-11; 
Tijsterman, M., Plasterk, R.H., (2004) Cell, 117 (1), pp. 1-3; 
De Fougerolles, A., Vornlocher, H.P., Maraganore, J., Lieberman, J., (2007) Nat. Rev. Drug Discovery, 6 (6), pp. 443-53; 
Shrivastava, N., Srivastava, A., (2008) Biotechnol. J., 3 (3), pp. 339-53; 
Soutschek, J., Akinc, A., Bramlage, B., Charisse, K., Constien, R., Donoghue, M., Elbashir, S., Vornlocher, H.P., (2004) Nature, 432 (7014), pp. 173-8; 
Takeshita, F., Ochiya, T., (2006) Cancer Sci., 97 (8), pp. 689-96; 
Bitko, V., Musiyenko, A., Shulyayeva, O., Barik, S., (2005) Nat. Med., 11 (1), pp. 50-5; 
Fjose, A., Drivenes, O., (2006) Birth Defects Res., C, 78 (2), pp. 150-71; 
Whitehead, K.A., Langer, R., Anderson, D.G., (2009) Nat. Rev. Drug Discovery, 8 (2), pp. 129-38; 
Liu, X., Howard, K.A., Dong, M., Andersen, M.O., Rahbek, U.L., Johnsen, M.G., Hansen, O.C., Kjems, J., (2007) Biomaterials, 28 (6), pp. 1280-8; 
Howard, K.A., Rahbek, U.L., Liu, X., Damgaard, C.K., Glud, S.Z., Andersen, M.O., Hovgaard, M.B., Kjems, J., (2006) Mol. Ther., 14 (4), pp. 476-84; 
Sell, S.A., McClure, M.J., Barnes, C.P., Knapp, D.C., Walpoth, B.H., Simpson, D.G., Bowlin, G.L., (2006) Biomed. Mater., 1 (2), pp. 72-80; 
Janes, K.A., Calvo, P., Alonso, M.J., (2001) Adv. Drug Delivery Rev., 47 (1), pp. 83-97; 
Pouton, C.W., Seymour, L.W., (2001) Adv. Drug Delivery Rev., 46 (1-3), pp. 187-203; 
Nel, A.E., Madler, L., Velegol, D., Xia, T., Hoek, E.M., Somasundaran, P., Klaessig, F., Thompson, M., (2009) Nat. Mater., 8 (7), pp. 543-57; 
Ishii, T., Okahata, Y., Sato, T., (2001) Biochim. Biophys. Acta, 1514 (1), pp. 51-64; 
Huang, M., Fong, C.W., Khor, E., Lim, L.Y., (2005) J. Controlled Release, 106 (3), pp. 391-406; 
Richardson, S.C., Kolbe, H.V., Duncan, R., (1999) Int. J. Pharm., 178 (2), pp. 231-43; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., (2003) Biomaterials, 24 (7), pp. 1255-64; 
Moghimi, S.M., Symonds, P., Murray, J.C., Hunter, A.C., Debska, G., Szewczyk, A., (2005) Mol. Ther., 11 (6), pp. 990-5; 
Koping-Hoggard, M., Tubulekas, I., Guan, H., Edwards, K., Nilsson, M., Varum, K.M., Artursson, P., (2001) Gene Ther., 8 (14), pp. 1108-21; 
Moreira, C., Oliveira, H., Pires, L.R., Simoes, S., Barbosa, M.A., Pego, A.P., (2009) Acta Biomater., 5 (8), pp. 2995-3006; 
Lietz, M., Dreesmann, L., Hoss, M., Oberhoffner, S., Schlosshauer, B., (2006) Biomaterials, 27 (8), pp. 1425-36; 
Ribeiro-Resende, V.T., Koenig, B., Nichterwitz, S., Oberhoffner, S., Schlosshauer, B., (2009) Biomaterials, 30 (29), pp. 5251-9; 
Andersen, M.O., Howard, K.A., Paludan, S.R., Besenbacher, F., Kjems, J., (2008) Biomaterials, 29 (4), pp. 506-12; 
Anchordoquy, T.J., Allison, S.D., Molina, M.C., Girouard, L.G., Carson, T.K., (2001) Drug Discovery Today, 6 (9), pp. 463-470; 
Atala, A., Bauer, S.B., Soker, S., Yoo, J.J., Retik, A.B., (2006) Lancet, 367 (9518), pp. 1241-6; 
Lin, C.Y., Schek, R.M., Mistry, A.S., Shi, X., Mikos, A.G., Krebsbach, P.H., Hollister, S.J., (2005) Tissue Eng., 11 (9-10), pp. 1589-98; 
Suggate, E.L., Ahmed, Z., Read, M.L., Eaton-Charnock, K., Douglas, M.R., Gonzalez, A.M., Berry, M., Logan, A., (2009) Mol. Cell Neurosci., 40 (4), pp. 451-62; 
Ahmed, Z., Dent, R.G., Suggate, E.L., Barrett, L.B., Seabright, R.J., Berry, M., Logan, A., (2005) Mol. Cell Neurosci., 28 (3), pp. 509-23; 
Fournier, A.E., Takizawa, B.T., Strittmatter, S.M., (2003) J. Neurosci., 23 (4), pp. 1416-23
},
	month = {2010///},
	pages = {3933 - 3939},
	abstract = {Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75\% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal, Animals, article, Cell Line, cell viability, chemistry, Chitin, chitosan, cytology, Drug delivery, genetic transfection, genetics, Local delivery, metabolism, Microstructured, nanobiofunctionalized implants, nanoparticle, nanoparticles, nerve cell, nerve regeneration, Nerve repair, neurite, Neurite outgrowth, Neurites, Neurons, Polymer filaments, Prostheses and Implants, prostheses and orthoses, rhoA GTP-Binding Protein, RhoA guanine nucleotide binding protein, RNA, RNA, Small Interfering, siRNA, small interfering RNA, Spinal cords, Transfection, ultrastructure},
	isbn = {15306984 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77958033530\&partnerID=40\&md5=6862e82e01c3aedf5423ebb14248c6ec},
	author = {Mittnacht, U. and Hartmann, H. and Hein, S. and Oliveira, H. and Dong, M. and P{\^e}go, A.P. and Kjems, J. and Howard, K.A. and Schlosshauer, B.}
}
@article {2278,
	title = {Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {144},
	year = {2010},
	note = {Cited By (since 1996): 28Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2010.01.034PubMed ID: 20138937Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; CQM - Centro de Qu{\'\i}mica da Madeira, MMRG, Departamento de Qu{\'\i}mica, Universidade da Madeira, Campus Universit{\'a}rio da Penteada, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: lipid, 66455-18-3; nuclease, 9026-81-7; Cations; DNA, 9007-49-2; Dendrimers; Lipids; PAMAM StarburstReferences: Segura, T., Shea, L.D., Materials for non-viral gene delivery (2001) Annu. Rev. Mat. Res., 31, pp. 25-46;Mastrobattista, E., Bravo, S.A., van der Aa, M., Crommelin, D.J.A., Nonviral gene delivery systems: from simple transfection agents to artificial viruses (2005) Drug Discov. Today Technol., 2, pp. 103-109; 
Colella, P., Cotugno, G., Auricchio, A., Ocular gene therapy: current progress and future prospects (2009) Trends Mol. Med., 15, pp. 23-31; 
Scanlon, K.J., Cancer gene therapy: challenges and opportunities (2004) Anticancer Res., 24, pp. 501-504; 
Gersbach, C.A., Phillips, J.E., Garcia, A.J., Genetic engineering for skeletal regenerative medicine (2007) Annu. Rev. Biomed. Eng., 9, pp. 87-119; 
Heyde, M., Partridge, K.A., Oreffo, R.O., Howdle, S.M., Shakesheff, K.M., Garnett, M.C., Gene therapy used for tissue engineering applications (2007) J. Pharm. Pharmacol., 59, pp. 329-350; 
Gafni, Y., Turgeman, G., Liebergal, M., Pelled, G., Gazit, Z., Gazit, D., Stem cells as vehicles for orthopedic gene therapy (2004) Gene Ther., 11, pp. 417-426; 
Vaananen, H.K., Mesenchymal stem cells (2005) Ann. Med., 37, pp. 469-479; 
Phillips, J.E., Gersbach, C.A., Garcia, A.J., Virus-based gene therapy strategies for bone regeneration (2007) Biomaterials, 28, pp. 211-229; 
Lundstrom, K., Latest development in viral vectors for gene therapy (2003) Trends Biotechnol., 21, pp. 117-122; 
Noel, D., Gazit, D., Bouquet, C., Apparailly, F., Bony, C., Plence, P., Millet, V., Jorgensen, C., Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells (2004) Stem Cells, 22, pp. 74-85; 
Mintzer, M.A., Simanek, E.E., Nonviral vectors for gene delivery (2009) Chem. Rev., 109, pp. 259-302; 
Hamm, A., Krott, N., Breibach, I., Blindt, R., Bosserhoff, A.K., Efficient transfection method for primary cells (2002) Tissue Eng., 8, pp. 235-245; 
McMahon, J.M., Conroy, S., Lyons, M., Greiser, U., O{\textquoteright}Shea, C., Strappe, P., Howard, L., O{\textquoteright}Brien, T., Gene transfer into rat mesenchymal stem cells: a comparative study of viral and nonviral vectors (2006) Stem Cells Dev., 15, pp. 87-96; 
Jang, W.-D., Selim, K.M.K., Lee, C.-H., Kang, I.-K., Bioinspired application of dendrimers: from bio-mimicry to biomedical applications (2009) Prog. Polym. Sci., 34, pp. 1-23; 
Svenson, S., Tomalia, D.A., Dendrimers in biomedical applications-reflections on the field (2005) Adv. Drug Deliv. Rev., 57, pp. 2106-2129; 
Caminade, A.M., Turrin, C.O., Majoral, J.P., Dendrimers and DNA: combinations of two special topologies for nanomaterials and biology (2008) Chemistry, 14, pp. 7422-7432; 
Dufes, C., Uchegbu, I.F., Schatzlein, A.G., Dendrimers in gene delivery (2005) Adv. Drug Deliv. Rev., 57, pp. 2177-2202; 
Guillot, M., Eisler, S., Diederich, F., Dendritic vectors for gene transfection (2007) New J. Chem., 31, pp. 1111-1127; 
Lechardeur, D., Verkman, A.S., Lukacs, G.L., Intracellular routing of plasmid DNA during non-viral gene transfer (2005) Adv. Drug Deliv. Rev., 57, pp. 755-767; 
Smith, D.K., Dendrimers and the double helix - from DNA binding towards gene therapy (2008) Curr. Top. Med. Chem., 8, pp. 1187-1203; 
Dobrovolskaia, M.A., McNeil, S.E., Immunological properties of engineered nanomaterials (2007) Nat. Nanotechnol., 2, pp. 469-478; 
Kukowska-Latallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., Baker, J.R., Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers (1996) Proc. Natl. Acad. Sci. U.S.A., 93, pp. 4897-4902; 
Santos, J.L., Oramas, E., Pego, A.P., Granja, P.L., Tomas, H., Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors (2009) J. Control. Release, 134, pp. 141-148; 
Dennig, J., Duncan, E., Gene transfer into eukaryotic cells using activated polyamidoamine dendrimers (2002) J. Biotechnol., 90, pp. 339-347; 
Kono, K., Akiyama, H., Takahashi, T., Takagishi, T., Harada, A., Transfection activity of polyamidoamine dendrimers having hydrophobic amino acid residues in the periphery (2005) Bioconjug. Chem., 16, pp. 208-214; 
Takahashi, T., Harada, A., Emi, N., Kono, K., Preparation of efficient gene carriers using a polyamidoamine dendron-bearing lipid: improvement of serum resistance (2005) Bioconjug. Chem., 16, pp. 1160-1165; 
Takahashi, T., Kojima, C., Harada, A., Kono, K., Alkyl chain moieties of polyamidoamine dendron-bearing lipids influence their function as a nonviral gene vector (2007) Bioconjug. Chem., 18, pp. 1349-1354; 
Takahashi, T., Kono, K., Itoh, T., Emi, N., Takagishi, T., Synthesis of novel cationic lipids having polyamidoamine dendrons and their transfection activity (2003) Bioconjug. Chem., 14, pp. 764-773; 
Jones, S.P., Gabrielson, N.P., Pack, D.W., Smith, D.K., Synergistic effects in gene delivery - a structure-activity approach to the optimisation of hybrid dendritic-lipidic transfection agents (2008) Chem. Commun., 39, pp. 4700-4702; 
Shah, D.S., Sakthivel, T., Toth, I., Florence, A.T., Wilderspin, A.F., DNA transfection and transfected cell viability using amphipathic asymmetric dendrimers (2000) Int. J. Pharm., 208, pp. 41-48; 
Ahmad, A., Evans, H.M., Ewert, K., George, C.X., Samuel, C.E., Safinya, C.R., New multivalent cationic lipids reveal bell curve for transfection efficiency versus membrane charge density: lipid-DNA complexes for gene delivery (2005) J. Gene Med., 7, pp. 739-748; 
Ewert, K.K., Evans, H.M., Zidovska, A., Bouxsein, N.F., Ahmad, A., Safinya, C.R., A columnar phase of dendritic lipid-based cationic liposome-DNA complexes for gene delivery: hexagonally ordered cylindrical micelles embedded in a DNA honeycomb lattice (2006) J. Am. Chem. Soc., 128, pp. 3998-4006; 
Guillot, M., Eisler, S., Weller, K., Merkle, H.P., Gallani, J.L., Diederich, F., Effects of structural modification on gene transfection and self-assembling properties of amphiphilic dendrimers (2006) Org. Biomol. Chem., 4, pp. 766-769; 
Joester, D., Losson, M., Pugin, R., Heinzelmann, H., Walter, E., Merkle, H.P., Diederich, F., Amphiphilic dendrimers: novel self-assembling vectors for efficient gene delivery (2003) Angew. Chem. Int. Ed., 42, pp. 1486-1490; 
Alvarez, E., Girones, N., Davis, R.J., Inhibition of the receptor-mediated endocytosis of diferric transferrin is associated with the covalent modification of the transferrin receptor with palmitic acid (1990) J. Biol. Chem., 265, pp. 16644-16655; 
Jevprasesphant, R., Penny, J., Attwood, D., McKeown, N.B., D{\textquoteright}Emanuele, A., Engineering of dendrimer surfaces to enhance transepithelial transport and reduce cytotoxicity (2003) Pharm. Res., 20, pp. 1543-1550; 
Snyder, S.L., Sobocinski, P.Z., An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines (1975) Anal. Biochem., 64, pp. 284-288; 
Lapidot, Y., Rappoport, S., Wolman, Y., Use of esters of N-hydroxysuccinimide in the synthesis of N-acylamino acids (1967) J. Lipid. Res., 8, pp. 142-145; 
Gao, X., Huang, L., Potentiation of cationic liposome-mediated gene delivery by polycations (1996) Biochemistry, 35, pp. 1027-1036; 
Aubin, J.E., Osteoprogenitor cell frequency in rat bone marrow stromal populations: role for heterotypic cell-cell interactions in osteoblast differentiation (1999) J. Cell Biochem., 72, pp. 396-410; 
Perrot, S., Dutertre-Catella, H., Martin, C., Rat, P., Warnet, J.M., Resazurin metabolism assay is a new sensitive alternative test in isolated pig cornea (2003) Toxicol. Sci., 72, pp. 122-129; 
Hosseinkhani, H., Yamamoto, M., Inatsugu, Y., Hiraoka, Y., Inoue, S., Shimokawa, H., Tabata, Y., Enhanced ectopic bone formation using a combination of plasmid DNA impregnation into 3-D scaffold and bioreactor perfusion culture (2006) Biomaterials, 27, pp. 1387-1398; 
Wong, S.Y., Pelet, J.M., Putnam, D., Polymer systems for gene delivery - past, present, and future (2007) Prog. Polym. Sci., 32, pp. 799-837; 
Abbasi, M., Uludag, H., Incani, V., Hsu, C.Y., Jeffery, A., Further investigation of lipid-substituted poly(L-lysine) polymers for transfection of human skin fibroblasts (2008) Biomacromolecules, 9, pp. 1618-1630; 
Sorgi, F.L., Bhattacharya, S., Huang, L., Protamine sulfate enhances lipid-mediated gene transfer (1997) Gene Ther., 4, pp. 961-968; 
Lampela, P., Soininen, P., Urtti, A., M{\"a}nnist{\"o}, P.T., Raasmaja, A., Synergism in gene delivery by small PEIs and three different nonviral vectors (2004) Int. J. Pharm., 270, pp. 175-184; 
Brito, L., Little, S., Langer, R., Amiji, M., Poly(β-amino ester) and cationic phospholipid-based lipopolyplexes for gene delivery and transfection in human aortic endothelial and smooth muscle cells (2008) Biomacromolecules, 9, pp. 1179-1187; 
Hardy, J.G., Love, C.S., Gabrielson, N.P., Pack, D.W., Smith, D.K., Synergistic effects on gene delivery - co-formulation of small disulfide-linked dendritic polycations with Lipofectamine 2000TM (2009) Org. Biomol. Chem., 7, pp. 789-793; 
Callow, P., Fragneto, G., Cubitt, R., Barlow, D.J., Lawrence, M.J., Timmins, P., Interaction of cationic lipid vesicles with model cell membranes - as determined by neutron reflectivity (2005) Langmuir, 21, pp. 7912-7920; 
Malik, N., Wiwattanapatapee, R., Klopsch, R., Lorenz, K., Frey, H., Weener, J.W., Meijer, E.W., Duncan, R., Dendrimers: relationship between structure and biocompatibility in vitro, and preliminary studies on the biodistribution of 125I-labelled polyamidoamine dendrimers in vivo (2000) J. Control. Release, 65, pp. 133-148
},
	month = {2010///},
	pages = {55 - 64},
	abstract = {A new family of gene delivery vectors is synthesized consisting of a medium-size generation PAMAM dendrimer (generation 5, with amine termini) core randomly linked at the periphery to hydrophobic chains that vary in length (12 to 16 carbon alkyl chains) and number (from 4.2 to 9.7 in average). The idea subjacent to the present work is to join the advantages of the cationic nature of the dendrimer with the capacity of lipids to interact with biological membranes. Unlike other amphiphilic systems designed for the same purpose, where the hydrophobic and hydrophilic moieties coexist in opposite sides, the present vectors have a hydrophilic interior and a hydrophobic corona. The vectors are characterized in respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA are analyzed concerning their size, ζ-potential, resistance to serum nucleases, capacity of being internalized by cells and transfection efficiency. These new vectors show a remarkable capacity for mediating the internalization of pDNA with minimum cytotoxicity, being this effect positively correlated with the -CH                        2- content present in the hydrophobic corona. Gene expression in MSCs, a cell type with relevancy in the regenerative medicine clinical context, is also enhanced using the new vectors but, in this case, the higher efficiency is shown by the vectors containing the smallest hydrophobic chains. {\textcopyright} 2010 Elsevier B.V.
},
	keywords = {amphophile, animal cell, animal experiment, article, Biological membranes, Biomedical applications, cation, Cations, Cell culture, cell membrane transport, cell type, Cellular uptake, controlled study, Cytotoxicity, dendrimer, Dendrimers, DNA, DNA binding, Dyes, Functional polymers, Gene delivery, gene delivery system, gene expression, Gene therapy, Gene transfer, Gene Transfer Techniques, gene vector, Genes, genetic transfection, Genetic Vectors, Humans, Hydrocarbons, hydrophilicity, hydrophobicity, internalization, lipid, Lipids, male, Mesenchymal stem cell, Mesenchymal Stem Cells, nonhuman, nuclease, Nucleic acids, plasmid DNA, Plasmids, Poly(amidoamine) dendrimers, polyamidoamine, Polyamidoamine dendrimers, priority journal, rat, Regenerative Medicine, Stem cells, Transfection, Vectors, Zeta potential},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953239685\&partnerID=40\&md5=51dd40fc4a666fd362b5eaa2447703dd},
	author = {Santos, J.L. and Oliveira, H. and Pandita, D. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
@article {2254,
	title = {Protein matrices for improved wound healing: Elastase inhibition by a synthetic peptide model},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {11},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm100537bPubMed ID: 20690591Language of Original Document: EnglishCorrespondence Address: Vasconcelos, A.; Universidade Do Minho, Departamento de Engenharia T{\^e}xtil, Campus de Azur{\'e}m, 4800-058 Guimar{\~a}es, Portugal; email: andreiav@det.uminho.ptChemicals/CAS: Bowman Birk inhibitor, 37330-34-0; Fibroins, 9007-76-5; Keratins, 68238-35-7; Pancreatic Elastase, 3.4.21.36; Peptide Fragments; Trypsin Inhibitor, Bowman-Birk Soybean; Trypsin Inhibitors; fibroin, silkwormReferences: Boateng, J.S., Matthews, K.H., Stevens, H.N.E., Eccleston, G.M., Wound healing dressings and drug delivery systems: A review (2008) J. Pharm. Sci., 97, pp. 2892-2923;Rothe, M., Falanga, V., Growth factors: Their biology and promise in dermatologic diseases and tissue repair (1989) AMA Arch. Dermatol., 125, pp. 1390-1398; 
Shakespeare, P., Burn wound healing and skin substitutes (2001) Burns, 27, pp. 517-522; 
Martin, C.R., Wound infection: A failure of wound healing caused by an imbalance of bacteria (1997) Surg. Clin. North Am., 77, pp. 637-650; 
Stadelmann, W.K., Digenis, A.G., Tobin, G.R., Physiology and healing dynamics of chronic cutaneous wounds (1998) Am. J. Surg., 176, pp. 26S-38S; 
Eaglstein Wh, F.V., Chronic wounds (1997) Surg. Clin. North Am., 77, pp. 689-700; 
Gray, D.W.R., The wound exudate continuum: An aid to wound assessment (2004) Wounds U.K.: Appl. Wound Manageme. Suppl., pp. 19-21; 
Chen, W.Y.J., Rogers, A.A., Lydon, M.J., Characterization of biologic properties of wound fluid collected during early stages of wound healing (1992) J. Invest. Dermatol., 99, pp. 559-564; 
Brooke, B., Edward, J.C., Caroline, A.O., Leukocyte proteinases in wound healing: Roles in physiologic and pathologic processes (1999) Wound Repair Regener., 7, pp. 410-422; 
Dorne, R.Y., Benedict, C.N., The proteolytic environment of chronic wounds (1999) Wound Repair Regener., 7, pp. 433-441; 
Naomi, J.T., Michael, C.S., Shawn, M., Neil, B., Jane, G., Frank, B., Gillian, M., Gregory, S., Analysis of the acute and chronic wound environments: The role of proteases and their inhibitors (1999) Wound Repair Regener., 7, pp. 442-452; 
Enoch, S., Leaper, D.J., Basic science of wound healing (2008) Surgery (Oxford), 26, pp. 31-37; 
Chufa, H., Margaret, A.H., George, W.C., Frank, A., Effects of chronic wound fluid on the bioactivity of platelet-derived growth factor in serum-free medium and its direct effect on fibroblast growth (1999) Wound Repair Regener., 7, pp. 97-105; 
Chen, P., Rose, J., Love, R., Wei, C.H., Wang, B.C., Reactive sites of an anticarcinogenic Bowman-Birk proteinase inhibitor are similar to other trypsin inhibitors (1992) J. Biol. Chem., 267, pp. 1990-1994; 
Werner, M.H., Wemmer, D.E., Three-dimensional structure of soybean trypsin/chymotrypsin Bowman-Birk inhibitor in solution (1992) Biochemistry, 31, pp. 999-1010; 
Guangda, L.I.N., Wolfram, B., Robert, H., Chengwu, C.H.I., Richard, A.E., The 0.25-nm X-ray structure of the Bowman-Birk-type inhibitor from mung bean in ternary complex with porcine trypsin (1993) Eur. J. Biochem., 212, pp. 549-555; 
Voss, R.-H., Ulrich, E., Lars-Oliver, E., Gabriele, W., Young-Mi, K., Peter, F., Crystal structure of the bifunctional soybean Bowman-Birk inhibitor at 0.28-nm resolution (1996) Eur. J. Biochem., 242, pp. 122-131; 
Wolfram, B., Robert, H., Natural protein proteinase inhibitors and their interaction with proteinases (1992) Eur. J. Biochem., 204, pp. 433-451; 
Laskowski, M., Qasim, M.A., What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme substrate complexes (2000) Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol., 1477, pp. 324-337; 
McBride, J.D., Leatherbarrow, R.J., Synthetic peptide mimics of the Bowman-Birk inhibitor protein (2001) Curr. Med. Chem., 8, pp. 909-917; 
Jeffrey, D.M., Emma, M.W., Arnd, B.E.B., Agn{\`e}s, M.J., Robin, J.L., Peptide mimics of the Bowman-Birk inhibitor reactive site loop (2002) Pept. Sci., 66, pp. 79-92; 
Vasconcelos, A., Freddi, G., Cavaco-Paulo, A., Biodegradable materials based on silk fibroin and keratin (2008) Biomacromolecules, 9, pp. 1299-1305; 
Ritger, P.L., Peppas, N.A., A simple equation for description of solute release II. Fickian and anomalous release from swellable devices (1987) J. Controlled Release, 5, pp. 37-42; 
Tanaka, H., Shimazu, T., Sugimoto, H., Yoshioka, T., Sugimoto, T., A sensitive and specific assay for granulocyte elastase in inflammatory tissue fluid using l-pyroglutamyl-l-prolyl-l-valine-p-nitroanilide (1990) Clin. Chim. Acta, 187, pp. 173-180; 
Yamauchi, K., Maniwa, M., Mori, T., Cultivation of fibroblast cells on keratin-coated substrata (1998) J. Biomater. Sci., Polym. Ed., 9, pp. 259-270; 
Li, M., Ogiso, M., Minoura, N., Enzymatic degradation behavior of porous silk fibroin sheets (2003) Biomaterials, 24, pp. 357-365; 
Minoura, N., Aiba, S., Higuchi, M., Gotoh, Y., Tsukada, M., Imai, Y., (1995) Biochem. Biophys. Res. Commun., 208, p. 511; 
Yamauchi, K., Hojo, H., Yamamoto, Y., Tanabe, T., Enhanced cell adhesion on RGDS-carrying keratin film (2003) Mater. Sci. Eng., C, 23, pp. 467-472; 
Roh, D.-H., Kang, S.-Y., Kim, J.-Y., Kwon, Y.-B., Young Kweon, H., Lee, K.-G., Park, Y.-H., Lee, J.-H., Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat (2006) J. Mater. Sci.: Mater. Med., 17, pp. 547-552; 
Lu, S., Wang, X., Lu, Q., Hu, X., Uppal, N., Omenetto, F.G., Kaplan, D.L., Stabilization of Enzymes in Silk Films (2009) Biomacromolecules, 10, pp. 1032-1042; 
Hofmann, S., Wong Po Foo, C.T., Rossetti, F., Textor, M., Vunjak-Novakovic, G., Kaplan, D.L., Merkle, H.P., Meinel, L., Silk fibroin as an organic polymer for controlled drug delivery (2006) J. Controlled Release, 111, pp. 219-227; 
Numata, K., Cebe, P., Kaplan, D.L., Mechanism of enzymatic degradation of beta-sheet crystals (2010) Biomaterials, 31, pp. 2926-2933; 
Shudong, W., Youzhu, Z., Guibo, Y., Hongwei, W., Zhihui, D., Electrospun polylactide/silk fibroin-gelatin composite tubular scaffolds for small-diameter tissue engineering blood vessels (2009) J. Appl. Polym. Sci., 113, pp. 2675-2682; 
Motta, A., Migliaresi, C., Faccioni, F., Torricelli, P., Fini, M., Giardino, R., Fibroin hydrogels for biomedical applications: Preparation, characterization and in vitro cell culture studies (2004) J. Biomater. Sci., Polym. Ed., 15, p. 851; 
Bondar, B., Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Functionality of endothelial cells on silk fibroin nets: Comparative study of micro-and nanometric fibre size (2008) Biomaterials, 29, pp. 561-572; 
Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials (2006) Biomaterials, 27, pp. 5399-5408; 
Unger, R.E., Wolf, M., Peters, K., Motta, A., Migliaresi, C., James Kirkpatrick, C., Growth of human cells on a non-woven silk fibroin net: A potential for use in tissue engineering (2004) Biomaterials, 25, pp. 1069-1075; 
Huang, Y., Yu, H., Xiao, C., PH-sensitive cationic guar gum/poly(acrylic acid) polyelectrolyte hydrogels: Swelling and in vitro drug release (2007) Carbohydr. Polym., 69, pp. 774-783; 
Edwards, J.V., Alvin, F.B., Sarah, B., Abul, J.U., Kelman, C.I., Robert, F.D., Steven, J.M., Inhibition of elastase by a synthetic cotton-bound serine protease inhibitor: In vitro kinetics and inhibitor release (1999) Wound Repair Regener., 7, pp. 106-118; 
Schneider, A., Wang, X.Y., Kaplan, D.L., Garlick, J.A., Egles, C., Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing (2009) Acta Biomater., 5, pp. 2570-2578; 
Gariani, T., McBride, J.D., Leatherbarrow, R.J., The role of the P2' position of Bowman-Birk proteinase inhibitor in the inhibition of trypsin: Studies on P2' variation in cyclic peptides encompassing the reactive site loop (1999) Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol., 1431, pp. 232-237; 
Zablotna, E., Kazmierczak, K., Jaskiewicz, A., Stawikowski, M., Kupryszewski, G., Rolka, K., Chemical synthesis and kinetic study of the smallest naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues (2002) Biochem. Biophys. Res. Commun., 292, pp. 855-859
},
	month = {2010///},
	pages = {2213 - 2220},
	abstract = {The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman-Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase-antielastase imbalance found on chronic wounds. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal cell, Animals, article, Atomic force microscopy, Biodegradation, Biological degradation, Biological functions, biomaterial, Bombyx, Bowman Birk inhibitor, Bowman-Birk inhibitors, cell proliferation, Chronic wounds, controlled study, Cytotoxicity test, Degradation, Elastase, elastase inhibitor, enzyme activity, Enzyme inhibition, Fibroins, Film composition, In-vitro, keratin, Keratins, mass spectrometry, materials testing, Mice, Microscopy, Atomic Force, NIH 3T3 Cells, nonhuman, pancreatic elastase, Peptide Fragments, Peptides, priority journal, protein binding, Protein matrices, Release rate, Silk, Silk fibroin, Spectrometry, Mass, Electrospray Ionization, Sus, Swine, Synthetic peptide, Trypsin Inhibitor, Bowman-Birk Soybean, Trypsin Inhibitors, wound fluid, Wound healing},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956552375\&partnerID=40\&md5=7d7dc9145b9655d9ba0461eb7a85d118},
	author = {Vasconcelos, A. and P{\^e}go, A.P. and Henriques, L. and Lamghari, M. and Cavaco-Paulo, A.}
}
@article {2272,
	title = {Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells},
	journal = {Molecular PharmaceuticsMol. Pharm.},
	volume = {7},
	year = {2010},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1021/mp9002877PubMed ID: 20230026Language of Original Document: EnglishCorrespondence Address: Balian, G.; Orthopaedic Research Laboratories, Department of Orthopaedics and Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, United States; email: lenat@uma.ptChemicals/CAS: Dendrimers; Green Fluorescent Proteins, 147336-22-9; PAMAM Starburst; Peptides; enhanced green fluorescent proteinReferences: V{\"a}{\"a}n{\"a}nen, H.K., Mesenchymal stem cells (2005) Ann. Med., 37, pp. 469-479;Caplan, A.I., Adult mesenchymal stem cells for tissue engineering versus regenerative medicine (2007) J. Cell Physiol., 213, pp. 341-347; 
Kay, M.A., Glorioso, J.C., Naldini, L., Viral vectors for gene therapy: The art of turning infectious agents into vehicles of therapeutics (2001) Nat. Med., 7, pp. 33-40; 
Mastrobattista, E., Bravo, S.A., Van Der Aa, M., Crommelin, D.J.A., Nonviral gene delivery systems: From simple transfection agents to artificial viruses (2005) Drug Discovery Today: Technol., 2, pp. 103-109; 
Merdan, T., Kopecek, J., Kissel, T., Prospects for cationic polymers in gene and oligonucleotide therapy against cancer (2002) Adv. Drug Delivery Rev., 54, pp. 715-758; 
Hamm, A., Krott, N., Breibach, I., Blindt, R., Bosserhoff, A.K., Efficient transfection method for primary cells (2002) Tissue Eng., 8, pp. 235-245; 
McMahon, J.M., Conroy, S., Lyons, M., Greiser, U., O{\textquoteright}Shea, C., Strappe, P., Howard, L., O{\textquoteright}Brien, T., Gene transfer into rat mesenchymal stem cells: A comparative study of viral and nonviral vectors (2006) Stem Cells Dev., 15, pp. 87-96; 
Svenson, S., Tomalia, D.A., Dendrimers in biomedical applications-reflections on the field (2005) Adv. Drug Delivery Rev., 57, pp. 2106-2129; 
Duf-s, C., Uchegbu, I.F., Schatzlein, A.G., Dendrimers in gene delivery (2005) Adv. Drug Delivery Rev., 57, pp. 2177-2202; 
Guillot, M., Eisler, S., Diederich, F., Dendritic vectors for gene transfection (2007) New J. Chem., 31, pp. 1111-1127; 
Smith, D.K., Dendrimers and the double helix-from DNA binding towards gene therapy (2008) Curr. Top. Med. Chem., 8, pp. 1187-1203; 
Kukowska-Latallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., Baker Jr., J.R., Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers (1996) Proc. Natl. Acad. Sci. U.S.A., 93, pp. 4897-4902; 
Santos, J.L., Oramas, E., P{\^e}go, A.P., Granja, P.L., Tom{\'a}s, H., Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors (2009) J. Controlled Release, 134, pp. 141-148; 
Balian, G., (2008), Bone Targeting Peptides. In United States Patent 7,323,542, issued January 29Snyder, S.L., Sobocinski, P.Z., An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines (1975) Anal. Biochem., 64, pp. 284-288; 
Haensler, J., Szoka Jr., F.C., Polyamidoamine cascade polymers mediate efficient transfection of cells in culture (1993) Bioconjugate Chem., 4, pp. 372-379; 
Hosseinkhani, H., Yamamoto, M., Inatsugu, Y., Hiraoka, Y., Inoue, S., Shimokawa, H., Tabata, Y., Enhanced ectopic bone formation using a combination of plasmid DNA impregnation into 3-D scaffold and bioreactor perfusion culture (2006) Biomaterials, 27, pp. 1387-1398; 
Perrot, S., Dutertre-Catella, H., Martin, C., Rat, P., Warnet, J.M., Resazurin metabolism assay is a new sensitive alternative test in isolated pig cornea (2003) Toxicol. Sci., 72, pp. 122-129; 
Wood, K.C., Little, S.R., Langer, R., Hammond, P.T., A family of hierarchically self-assembling linear-dendritic hybrid polymers for highly efficient targeted gene delivery (2005) Angew. Chem., Int. Ed., 44, pp. 6704-6708; 
Dan Luo, K.H., Belcheva, N., Han, E., Mark Saltzman, W., Poly(ethylene glycol)-conjugated PAMAM dendrimer for biocompatible, high-efficiency dna delivery (2002) Macromolecules, 35, pp. 3456-3462; 
Schaffer, D.V., Lauffenburger, D.A., Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery (1998) J. Biol. Chem., 273, pp. 28004-28009; 
Santos, J.L., Oliveira, H., Pandita, D., P{\^e}go, A.P., Granja, P.L., Tom{\'a}s, H., Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells (2010) J. Controlled Release, , doi: 10.1016/j.jconrel.2010.01.034
},
	month = {2010///},
	pages = {763 - 774},
	abstract = {As mesenchymal stem cells (MSCs) can differentiate into multiple cell types, the delivery of exogenous genes to this type of cell can be an important tool in tissue regeneration and engineering. However transfection of MSCs using nonviral gene delivery vectors is difficult, the development of more efficient and safe DNA vehicles being necessary. Moreover, specific transfection of MSCs may be required to avoid unwanted side effects in other tissues. In this study, a novel family of gene delivery vectors based on poly(amidoamine) (PAMAM) dendrimers functionalized with peptides displaying high affinity toward MSCs was prepared. The vectors were characterized with respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA were analyzed concerning their size, -potential, capacity of being internalized by cells and transfection efficiency. These new vectors exhibited low cytotoxicity, receptor-mediated gene delivery into MSCs and transfection efficiencies superior to those presented by native dendrimers and by partially degraded dendrimers. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal cell, animal experiment, animal tissue, Animals, article, asparaginylserylmethionylisoleucylalanylhistidylasparaginyllysylthreonylarginylmethionylhistidylglycylglycylglycylserylcysteine, Cell recognition, cell survival, Cells, Cultured, controlled study, Cytotoxicity, dendrimer, Dendrimers, DNA, DNA binding, Electrophoresis, Agar Gel, flow cytometry, Gene delivery, gene targeting, Gene Transfer Techniques, gene vector, genetic transfection, Genetic Vectors, Green Fluorescent Proteins, male, Mesenchymal stem cell, Mesenchymal Stem Cells, Models, Theoretical, multigene family, nonhuman, PAMAM dendrimers, peptide, Peptides, plasmid DNA, polyamidoamine, priority journal, rat, Rats, Rats, Wistar, serylglycylhistidylglutaminylleucylleucylleucylasparaginyllysylmethionylprolylasparaginylglycylglycylglycylserylcysteine, unclassified drug, Zeta potential},
	isbn = {15438384 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953253240\&partnerID=40\&md5=e06048334c689e2faa61e7b6b8ad9bf1},
	author = {Santos, J.L. and Pandita, D. and Rodrigues, J. and P{\^e}go, A.P. and Granja, P.L. and Balian, G. and Tom{\'a}s, H.}
}
@article {2275,
	title = {Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {143},
	year = {2010},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2010.01.018PubMed ID: 20093157Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; macrogol, 25322-68-3; polyethyleneimine, 74913-72-7; DNA, 9007-49-2; Imines; Peptide Fragments; Polyethylenes; Tetanus Toxin; poly(ethylene imine); tetanus toxin fragment CReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov. Today, 1 (2), pp. 177-185;Sahenk, Z., Neurotrophins and peripheral neuropathies (2006) Brain Pathol., 16 (4), pp. 311-319; 
Apfel, S.C., Kessler, J.A., Neurotrophic factors in the therapy of peripheral neuropathy (1995) Baillieres Clin. Neurol., 4 (3), pp. 593-606; 
Apfel, S.C., Is the therapeutic application of neurotrophic factors dead? (2002) Ann. Neurol., 51 (1), pp. 8-11; 
Glorioso, J.C., Mata, M., Fink, D.J., Therapeutic gene transfer to the nervous system using viral vectors (2003) J. Neurovirol., 9 (2), pp. 165-172; 
Boussif, O., Lezoualc{\textquoteright}h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B., A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine (1995) Proc. Natl. Acad. Sci. U.S.A., 92 (16), pp. 7297-7301; 
Coll, J.L., Chollet, P., Brambilla, E., Desplanques, D., Behr, J.P., Favrot, M., In vivo delivery to tumors of DNA complexed with linear polyethylenimine (1999) Hum. Gene Ther., 10 (10), pp. 1659-1666; 
Schaffer, D.V., Lauffenburger, D.A., Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery (1998) J. Biol. Chem., 273 (43), pp. 28004-28009; 
Zanta, M.A., Boussif, O., Adib, A., Behr, J.P., In vitro gene delivery to hepatocytes with galactosylated polyethylenimine (1997) Bioconjug. Chem., 8 (6), pp. 839-844; 
Diebold, S.S., Lehrmann, H., Kursa, M., Wagner, E., Cotten, M., Zenke, M., Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection (1999) Hum. Gene Ther., 10 (5), pp. 775-786; 
Blessing, T., Kursa, M., Holzhauser, R., Kircheis, R., Wagner, E., Different strategies for formation of PEGylated EGF-conjugated PEI/DNA complexes for targeted gene delivery (2001) Bioconjug. Chem., 12 (4), pp. 529-537; 
Erbacher, P., Remy, J.S., Behr, J.P., Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway (1999) Gene Ther., 6 (1), pp. 138-145; 
Kircheis, R., Kichler, A., Wallner, G., Kursa, M., Ogris, M., Felzmann, T., Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery (1997) Gene Ther., 4 (5), pp. 409-418; 
Park, I.K., Lasiene, J., Chou, S.H., Horner, P.J., Pun, S.H., Neuron-specific delivery of nucleic acids mediated by Tet1-modified poly(ethylenimine) (2007) J. Gene Med., 9 (8), pp. 691-702; 
Kwon, E.J., Bergen, J.M., Park, I.K., Pun, S.H., Peptide-modified vectors for nucleic acid delivery to neurons (2008) J. Control. Release, 132 (3), pp. 230-235; 
Price, D.L., Griffin, J., Young, A., Peck, K., Stocks, A., Tetanus toxin: direct evidence for retrograde intraaxonal transport (1975) Science, 188 (4191), pp. 945-947; 
Figueiredo, D.M., Hallewell, R.A., Chen, L.L., Fairweather, N.F., Dougan, G., Savitt, J.M., Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport (1997) Exp. Neurol., 145 (2 PART 1), pp. 546-554; 
Fishman, P.S., Savitt, J.M., Farrand, D.A., Enhanced CNS uptake of systemically administered proteins through conjugation with tetanus C-fragment (1990) J. Neurol. Sci., 98 (2-3), pp. 311-325; 
Coen, L., Osta, R., Maury, M., Brulet, P., Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system (1997) Proc. Natl. Acad. Sci. U.S.A., 94 (17), pp. 9400-9405; 
Knight, A., Carvajal, J., Schneider, H., Coutelle, C., Chamberlain, S., Fairweather, N., Non-viral neuronal gene delivery mediated by the HC fragment of tetanus toxin (1999) Eur. J. Biochem., 259 (3), pp. 762-769; 
Carlisle, R.C., Etrych, T., Briggs, S.S., Preece, J.A., Ulbrich, K., Seymour, L.W., Polymer-coated polyethylenimine/DNA complexes designed for triggered activation by intracellular reduction (2004) J. Gene Med., 6 (3), pp. 337-344; 
Goula, D., Remy, J.S., Erbacher, P., Wasowicz, M., Levi, G., Abdallah, B., Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system (1998) Gene Ther., 5 (5), pp. 712-717; 
Sinha, K., Box, M., Lalli, G., Schiavo, G., Schneider, H., Groves, M., Analysis of mutants of tetanus toxin Hc fragment: ganglioside binding, cell binding and retrograde axonal transport properties (2000) Mol. Microbiol., 37 (5), pp. 1041-1051; 
Ellman, G.L., Tissue sulfhydryl groups (1959) Arch. Biochem. Biophys., 82 (1), pp. 70-77; 
(1993) Iodine-125, a Guide to Radioionation Techniques, , Amersham Life Science; 
O{\textquoteright}brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) Eur. J. Biochem., 267 (17), pp. 5421-5426; 
Pollard, H., Remy, J.S., Loussouarn, G., Demolombe, S., Behr, J.P., Escande, D., Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells (1998) J. Biol. Chem., 273 (13), pp. 7507-7511; 
Neu, M., Fischer, D., Kissel, T., Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives (2005) J. Gene Med., 7 (8), pp. 992-1009; 
Moffatt, S., Papasakelariou, C., Wiehle, S., Cristiano, R., Successful in vivo tumor targeting of prostate-specific membrane antigen with a highly efficient J591/PEI/DNA molecular conjugate (2006) Gene Ther., 13 (9), pp. 761-772; 
Kircheis, R., Ostermann, E., Wolschek, M.F., Lichtenberger, C., Magin-Lachmann, C., Wightman, L., Tumor-targeted gene delivery of tumor necrosis factor-alpha induces tumor necrosis and tumor regression without systemic toxicity (2002) Cancer Gene Ther., 9 (8), pp. 673-680; 
Bellomo, G., Vairetti, M., Stivala, L., Mirabelli, F., Richelmi, P., Orrenius, S., Demonstration of nuclear compartmentalization of glutathione in hepatocytes (1992) Proc. Natl. Acad. Sci. U.S.A., 89 (10), pp. 4412-4416; 
Liu, H.S., Jan, M.S., Chou, C.K., Chen, P.H., Ke, N.J., Is green fluorescent protein toxic to the living cells? (1999) Biochem. Biophys. Res. Commun., 260 (3), pp. 712-717; 
Baens, M., Noels, H., Broeckx, V., Hagens, S., Fevery, S., Billiau, A.D., The dark side of EGFP: defective polyubiquitination (2006) PLoS One, 1, pp. e54; 
Taghizadeh, R.R., Sherley, J.L., CFP and YFP, but not GFP, provide stable fluorescent marking of rat hepatic adult stem cells (2008) J. Biomed. Biotechnol., 2008. , 453590; 
Liu, J.K., Tenga, Q.S., Garrity-Moses, M., Federici, T., Tanase, D., Imperiale, M.J., A novel peptide defined through phage display for therapeutic protein and vector neuronal targeting (2005) Neurobiol. Dis., 19 (3), pp. 407-418; 
Fishman, P.S., Carrigan, D.R., Retrograde transneuronal transfer of the C-fragment of tetanus toxin (1987) Brain Res., 406 (1-2), pp. 275-279
},
	month = {2010///},
	pages = {350 - 358},
	abstract = {A simple, safe and efficient system that can specifically transfect peripheral sensorial neurons can bring new answers to address peripheral neuropathies. A multi-component non-viral gene delivery vector targeted to peripheral nervous system cells was developed, using poly(ethylene imine) (PEI) as starting material. A binary DNA/polymer complex based on thiolated PEI (PEISH) was optimized, considering complex size and zeta potential and the ability to transfect a sensorial neuron cell line (ND7/23). The 50. kDa non-toxic fragment from tetanus toxin (HC), which has been previously shown to interact specifically with peripheral neurons and to undergo retrograde transport, was grafted to the complex core via a bifunctional PEG (HC-PEG) reactive for the thiol moieties present in the complex surface. Several formulations of HC-PEG ternary complexes were tested for targeting, by assessing the extent of cellular internalization and levels of transfection, in both the ND7/23 and NIH 3 T3 (fibroblast) cell lines. Targeted gene transfer to the neuronal cell line was observed for the complex formulations containing 5 and 7.5. Μg of HC-PEG. Finally, our results demonstrate that the developed ternary vectors are able to transfect primary cultures of dorsal root ganglion dissociated neurons in a targeted manner and elicit the expression of a relevant neurotrophic factor. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {Aberrations, animal cell, Animals, article, Bifunctional, Cell culture, Cell Line, Tumor, Cell lines, cell strain 3T3, Cells, Cultured, Cellular internalization, Chemical modification, Complex cores, Complex formulations, Complex surface, controlled study, DNA, DNA protein complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Female, Gene transfer, genetic transfection, Imines, internalization, macrogol, Mice, mouse, Multicomponents, nanoparticle, nanoparticles, neuroblastoma cell, Neuronal cell, Neurons, neurotrophic factor, Neurotrophic factors, NIH 3T3 Cells, Nitrogen compounds, Non-viral, Non-viral gene delivery, nonhuman, Nonviral, nonviral gene delivery system, Nucleic acids, Particle Size, Peptide Fragments, Peripheral nervous system, Peripheral neuropathy, Plasmids, Polyethylene Glycols, Polyethylene oxides, Polyethyleneimine, Polyethylenes, Primary culture, priority journal, process optimization, protein expression, rat, Rats, Rats, Wistar, Regenerative Medicine, Self-assembled, sensory nerve cell, spinal ganglion, Starting materials, Targeted, Targeted gene delivery, Ternary complex, Ternary vectors, Tetanus toxin, tetanus toxin fragment c, Transfection, unclassified drug, Zeta potential},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77952239026\&partnerID=40\&md5=077b03d49cf392defc3d0c77f2f249d1},
	author = {Oliveira, H. and Fernandez, R. and Pires, L.R. and Martins, M.C.L. and Sim{\~o}es, S. and Barbosa, M.A. and P{\^e}go, A.P.}
}
@article {2297,
	title = {Fibronectin-mediated endothelialisation of chitosan porous matrices},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2009.06.056PubMed ID: 19615736Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; fibronectin, 86088-83-7; Biocompatible Materials; Chitosan, 9012-76-4; FibronectinsReferences: Moon, J.J., West, J.L., Vascularization of engineered tissues: approaches to promote angiogenesis in biomaterials (2008) Current Topics in Medicinal Chemistry, 8 (4), pp. 300-310;Blitterswijk, C.A., Moroni, L., Rouwkema, J., Siddappa, R., Sohier, J., Tissue engineering - an introduction (2008) Tissue engineering, , Blitterswijk C.A. (Ed), Elsevier; 
Ehrbar, M., Zeisberger, S.M., Raeber, G.P., Hubbell, J.A., Schnell, C., Zisch, A.H., The role of actively released fibrin-conjugated VEGF for VEGF receptor 2 gene activation and the enhancement of angiogenesis (2008) Biomaterials, 29 (11), pp. 1720-1729; 
Elbjeirami, W.M., West, J.L., Angiogenesis-like activity of endothelial cells co-cultured with VEGF-producing smooth muscle cells (2006) Tissue Engineering, 12 (2), pp. 381-390; 
Unger, R.E., Peters, K., Wolf, M., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Endothelialization of a non-woven silk fibroin net for use in tissue engineering: growth and gene regulation of hunian endothelial cells (2004) Biomaterials, 25 (21), pp. 5137-5146; 
Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials (2006) Biomaterials, 27 (31), pp. 5399-5408; 
Kirkpatrick, C.J., Unger, R.E., Krump-Konvalinkova, V., Peters, K., Schmidt, H., Kamp, G., Experimental approaches to study vascularization in tissue engineering and biomaterial applications (2003) Journal of Materials Science, 14 (8), pp. 677-681; 
Calonder, C., Matthew, H.W.T., Van Tassel, P.R., Adsorbed layers of oriented fibronectin: a strategy to control cell-surface interactions (2005) Journal of Biomedical Materials Research Part A, 75 A (2), pp. 316-323; 
Kouvroukoglou, S., Dee, K.C., Bizios, R., McIntire, L.V., Zygourakis, K., Endothelial cell migration on surfaces modified with immobilized adhesive peptides (2000) Biomaterials, 21 (17), pp. 1725-1733; 
Burdick, J.A., Khademhosseini, A., Langer, R., Fabrication of gradient hydrogels using a microfluidics/photopolymerization process (2004) Langmuir, 20 (13), pp. 5153-5156; 
Garcia, A.J., Get a grip: integrins in cell-biomaterial interactions (2005) Biomaterials, 26, pp. 7525-7529; 
Tomihata, K., Ikada, Y., In vitro and in vivo degradation of films of chitin and its deacetylated derivatives (1997) Biomaterials, 18 (7), pp. 567-575; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cells osteogenic differentiation and fibronectin adsorption on chitosan membranes: the effect of the degree of acetylation (2005) Journal of Biomedical Materials Research Part A, 75 A (2), pp. 387-397; 
Nettles, D.L., Elder, S.H., Gilbert, J.A., Potential use of chitosan as a cell scaffold material for cartilage tissue engineering (2002) Tissue Engineering, 8 (6), pp. 1009-1016; 
Oliveira, S.M., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., Engineering endochondral bone: in vitro studies (2009) Tissue Engineering, 15 (3), pp. 625-634; 
Freier, T., Koh, H.S., Kazazian, K., Shoichet, M.S., Controlling cell adhesion and degradation of chitosan films by N-acetylation (2005) Biomaterials, 26 (29), pp. 5872-5878; 
Wang, X.H., Cui, F.Z., Feng, Q.L., Li, J.C., Zhang, Y.H., Preparation and characterization of collagen/chitosan matrices as potential biomaterials (2003) Journal of Bioactive and Compatible Polymers, 18 (6), pp. 453-467; 
Chupa, J.M., Foster, A.M., Sumner, S.R., Madihally, S.V., Matthew, H.W.T., Vascular cell responses to polysaccharide materials: in vitro and in vivo evaluations (2000) Biomaterials, 21 (22), pp. 2315-2322; 
Chung, T.W., Lu, Y.F., Wang, S.S., Lin, Y.S., Chu, S.H., Growth of human endothelial cells on photochemically grafted Gly-Arg-Gly-Asp (GRGD) chitosans (2002) Biomaterials, 23 (24), pp. 4803-4809; 
Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., Adhesive protein interactions with chitosan: consequences for valve endothelial cell growth on tissue-engineering materials (2003) Journal of Biomedical Materials Research Part A, 67 A (2), pp. 538-547; 
Huang, Y., Onyeri, S., Siewe, M., Moshfeghian, A., Madihally, S.V., In vitro characterization of chitosan-gelatin scaffolds for tissue engineering (2005) Biomaterials, 26 (36), pp. 7616-7627; 
Huang, Y., Siewe, M., Madilhally, S.V., Effect of spatial architecture on cellular colonization (2006) Biotechnology and Bioengineering, 93 (1), pp. 64-75; 
Kirsebom, H., Aguilar, M.R., Roman, J.S., Fernandez, M., Prieto, M.A., Bondar, B., Macroporous scaffolds based on chitosan and bioactive molecules (2007) Journal of Bioactive and Compatible Polymers, 22 (6), pp. 621-636; 
Cao, W.L., Jing, D.H., Li, J.M., Gong, Y.D., Zhao, N.M., Zhang, X.F., Effects of the degree of deacetylation on the physicochemical properties and Schwann cell affinity of chitosan films (2005) Journal of Biomaterials Applications, 20 (2), pp. 157-177; 
Amaral, I.F., Sampaio, P., Barbosa, M.A., Three-dimensional culture of human osteoblastic cells in chitosan sponges: the effect of the degree of acetylation (2006) Journal of Biomedical Materials Research Part A, 76 A (2), pp. 335-346; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42 (8), pp. 3569-3580; 
Vachoud, L., Zydowicz, N., Domard, A., Formation and characterisation of a physical chitin gel (1997) Carbohydrate Research, 302 (3-4), pp. 169-177; 
Montembault, A., Viton, C., Domard, A., Physico-chemical studies of the gelation of chitosan in a hydroalcoholic medium (2005) Biomaterials, 26 (8), pp. 933-943; 
Amaral, I.F., Cordeiro, A.L., Sampaio, P., Barbosa, M.A., Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation (2007) Journal of Biomaterials Science Polymer Edition, 18 (4), pp. 469-485; 
Krump-Konvalinkova, V., Bittinger, F., Unger, R.E., Peters, K., Lehr, H.A., Kirkpatrick, C.J., Generation of human pulmonary microvascular endothelial cell lines (2001) Laboratory Investigation, 81 (12), pp. 1717-1727; 
Unger, R.E., Krump-Konvalinkova, V., Peters, K., Kirkpatrick, C.J., In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R (2002) Microvascular Research, 64 (3), pp. 384-397; 
Jaffe, E.A., Nachman, R.L., Becker, C.G., Minick, C.R., Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria (1973) J Clin Invest, 52 (11), pp. 2745-2756; 
O{\textquoteright}Brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) European Journal of Biochemistry, 267 (17), pp. 5421-5426; 
Teixeira, C.C., Hatori, M., Leboy, P.S., Pacifici, M., Shapiro, I.M., A rapid and ultrasensitive method for measurement of DNA, calcium and protein-content, and alkaline-phosphatase activity of chondrocyte cultures (1995) Calcified Tissue International, 56 (3), pp. 252-256; 
Barbosa, J.N., Amaral, I.F., {\'A}guas, A.P., Barbosa, M.A., Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds Journal of Biomedical Materials Research Part A, , in press, doi:10.1002/jbm.a.32499; 
Marshall, A.J., Barker, T., Sage, E.H., Hauch, K.D., Ratner, B.D., (2004) Pore size controls angiogenesis in subcutaneously implanted porous matrices. 7th World Biomaterials Congress; 2004, , Australian Society for Biomaterials Inc., Sydney, Australia p. 710; 
Unger, R.E., Sartoris, A., Peters, K., Motta, A., Migliaresi, C., Kunkel, M., Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials (2007) Biomaterials, 28 (27), pp. 3965-3976; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13 (13), pp. 3404-3413; 
Wendt, D., Marsano, A., Jakob, M., Heberer, M., Martin, I., Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity (2003) Biotechnology and Bioengineering, 84 (2), pp. 205-214; 
Santos, M.I., Fuchs, S., Gomes, M.E., Unger, R.E., Reis, R.L., Kirkpatrick, C.J., Response of micro- and macrovascular endothelial cells to starch-based fiber meshes for bone tissue engineering (2007) Biomaterials, 28 (2), pp. 240-248; 
Vestweber, D., Molecular mechanisms that control endothelial cell contacts (2000) Journal of Pathology, 190 (3), pp. 281-291; 
Mori, T., Irie, Y., Nishimura, S., Tokura, S., Matsuura, M., Okumura, M., Endothelial cell responses to chitin and its derivatives (1998) Journal of Biomedical Materials Research, 43 (4), pp. 469-472; 
Panetti, T.S., Hannah, D.F., Avraamides, C., Gaughan, J.P., Marcinkiewicz, Z., Huttenlocher, A., Extracellular matrix molecules regulate endothelial cell migration stimulated by lysophosphatidic acid (2004) Journal of Thrombosis and Haemostasis, 2 (9), pp. 1645-1656; 
Garcia, A.J., Boettiger, D., Integrin-fibronectin interactions at the cell-material interface: initial integrin binding and signaling (1999) Biomaterials, 20 (23-24), pp. 2427-2433; 
Danen, E.H.J., Sonnenberg, A., Integrins in regulation of tissue development and function (2003) Journal of Pathology, 200 (4), pp. 471-480; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) Journal of Biomedical Materials Research Part A, 66 A (2), pp. 247-259; 
Renner, L., Jorgensen, B., Markowski, M., Salchert, K., Werner, C., Pompe, T., Control of fibronectin displacement on polymer substrates to influence endothelial cell behaviour (2004) Journal of Materials Science-Materials in Medicine, 15 (4), pp. 387-390; 
Garcia, A.J., Vega, M.D., Boettiger, D., Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation (1999) Molecular Biology of the Cell, 10 (3), pp. 785-798; 
Koenig, A.L., Gambillara, V., Grainger, D.W., Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates (2003) Journal of Biomedical Materials Research Part A, 64 A (1), pp. 20-37; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding (2004) Biomaterials, 25, pp. 5947-5954; 
Pompe, T., Kobe, F., Salchert, K., Jorgensen, B., Oswald, J., Werner, C., Fibronectin anchorage to polymer substrates controls the initial phase of endothelial cell adhesion (2003) Journal of Biomedical Materials Research Part A, 67 A (2), pp. 647-657
},
	month = {2009///},
	pages = {5465 - 5475},
	abstract = {Chitosan (Ch) porous matrices were investigated regarding their ability to be colonized by human microvascular endothelial cells (HPMEC-ST1.6R cell line) and macrovascular endothelial cells namely HUVECs. Specifically we assessed if previous incubation of Ch in a fibronectin (FN) solution was effective in promoting endothelial cell (EC) adhesion to Ch matrices with different degrees of acetylation (DAs). Upon FN physiadsorption, marked differences were found between the two DAs investigated, namely DA 4\% and 15\%. While cell adhesion was impaired on Ch with DA 15\%, ECs were able to not only adhere to Ch with DA 4\%, but also to spread and colonize the scaffolds, with retention of the EC phenotype and angiogenic potential. To explain the observed differences between the two DAs, protein adsorption studies using                        125I-FN and immunofluorescent labelling of FN cell-binding domains were carried out. In agreement with the higher cell numbers found, scaffolds with DA 4\% revealed a higher number of exposed FN cell-binding domains as well as greater ability to adsorb FN and to retain and exchange adsorbed FN in the presence of competitive proteins. These findings suggest that the DA is a key parameter modulating EC adhesion to FN-coated Ch by influencing the adsorbed protein layer. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.
},
	keywords = {Acetylation, Adhesion, Adsorption, angiogenesis, article, Biocompatible Materials, Blood vessel prostheses, cell adhesion, Cell culture, cell invasion, Cell Line, Cell lines, Cell membranes, Cell number, cell proliferation, Cell size, cell spreading, cell survival, Cell-binding domain, Chitin, Chitin/chitosan, chitosan, colony formation, controlled study, Degrees of acetylation, Electric conductivity measurement, Electric currents, Endothelial cell, Endothelial Cells, Endothelialisation, endothelium, endothelium cell, epithelization, Fibronectin, Fibronectins, human, human cell, Human microvascular endothelial cells, Humans, immunofluorescence test, Key parameters, Labels, materials testing, Matrix algebra, Neovascularization, Physiologic, phenotype, porosity, Porous matrixs, priority journal, Protein adsorption, Protein layers, Scaffold, Scaffolds, Self assembly, Tissue engineering, tissue scaffold, umbilical vein},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68549107574\&partnerID=40\&md5=9babb57c1a4f2ae6eb7e7e0643bffe48},
	author = {Amaral, I.F. and Unger, R.E. and Fuchs, S. and Mendon{\c c}a, A.M. and Sousa, S.R. and Barbosa, M.A. and P{\^e}go, A.P. and Kirkpatrick, C.J.}
}
@article {2296,
	title = {Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.04.021PubMed ID: 19427930Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; chitosan, 9012-76-4; imidazole, 1467-16-9, 288-32-4; Biocompatible Materials; Buffers; Chitosan, 9012-76-4; DNA, 9007-49-2; Drug CarriersReferences: Kirkpatrick, C.J., Fuchs, S., Peters, K., Brochhausen, C., Hermanns, M.I., Unger, R.E., Visions for regenerative medicine: interface between scientific fact and science fiction (2006) Artif Organs, 30, pp. 822-827;Sim{\~o}es, S., Filipe, A., Faneca, H., Mano, M., Penacho, N., Duzgunes, N., Cationic liposomes for gene delivery (2005) Expert Opin Drug Deliv, 2, pp. 237-254; 
Lungwitz, U., Breunig, M., Blunk, T., Gopferich, A., Polyethylenimine-based non-viral gene delivery systems (2005) Eur J Pharm Biopharm, 60, pp. 247-266; 
Filion, M.C., Phillips, N.C., Major limitations in the use of cationic liposomes for DNA delivery (1998) Int J Pharm, 162, pp. 159-170; 
Lv, H., Zhang, S., Wang, B., Cui, S., Yan, J., Toxicity of cationic lipids and cationic polymers in gene delivery (2006) J Control Release, 114, pp. 100-109; 
Itaka, K., Ohba, S., Miyata, K., Kawaguchi, H., Nakamura, K., Takato, T., Bone regeneration by regulated in vivo gene transfer using biocompatible polyplex nanomicelles (2007) Mol Ther, 15, pp. 1655-1662; 
Borchard, G., Chitosans for gene delivery (2001) Adv Drug Deliv Rev, 52, pp. 145-150; 
Khalil, I.A., Kogure, K., Akita, H., Harashima, H., Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery (2006) Pharmacol Rev, 58, pp. 32-45; 
Behr, J.P., The proton sponge: a trick to enter cells the viruses did not exploit (1997) Chimia, 51, pp. 34-36; 
Kim, T.H., Kim, S.I., Akaike, T., Cho, C.S., Synergistic effect of poly(ethylenimine) on the transfection efficiency of galactosylated chitosan/DNA complexes (2005) J Control Release, 105, pp. 354-366; 
Midoux, P., Monsigny, M., Efficient gene transfer by histidylated polylysine/pDNA complexes (1999) Bioconjug Chem, 10, pp. 406-411; 
Benns, J.M., Choi, J.S., Mahato, R.I., Park, J.S., Kim, S.W., pH-sensitive cationic polymer gene delivery vehicle: N-Ac-poly(l-histidine)-graft-poly(l-lysine) comb shaped polymer (2000) Bioconjug Chem, 11, pp. 637-645; 
Putnam, D., Gentry, C.A., Pack, D.W., Langer, R., Polymer-based gene delivery with low cytotoxicity by a unique balance of side-chain termini (2001) Proc Natl Acad Sci USA, 98, pp. 1200-1205; 
Dubruel, P., Christiaens, B., Vanloo, B., Bracke, K., Rosseneu, M., Vandekerckhove, J., Physicochemical and biological evaluation of cationic polymethacrylates as vectors for gene delivery (2003) Eur J Pharm Sci, 18, pp. 211-220; 
Dubruel, P., Dekie, L., Schacht, E., Poly-l-glutamic acid derivatives as multifunctional vectors for gene delivery. Part A. Synthesis and physicochemical evaluation (2003) Biomacromolecules, 4, pp. 1168-1176; 
Kim, T.H., Ihm, J.E., Choi, Y.J., Nah, J.W., Cho, C.S., Efficient gene delivery by urocanic acid-modified chitosan (2003) J Control Release, 93, pp. 389-402; 
Dubruel, P., Christiaens, B., Rosseneu, M., Vandekerckhove, J., Grooten, J., Goossens, V., Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery (2004) Biomacromolecules, 5, pp. 379-388; 
Juusola, P., Minkkinen, P., Leino, L., Laihia, J.K., Determination of the dissociation constants of urocanic acid isomers in aqueous solutions (2007) Monatsh Chem, 138, pp. 951-965; 
Roberts, J.D., Yu, C., Flanagan, C., Birdseye, T.R., A nitrogen-15 nuclear magnetic resonance study of the acid-base and tautomeric equilibria of 4-substituted imidazoles and its relevance to the catalytic mechanism of alfa-lytic protease (1982) J Am Chem Soc, 104, pp. 3945-3949; 
Roberts, G.A.F., (1992) Chitin chemistry, , Palgrave Macmillan, London; 
Nakagawa, Y., Murai, T., Hasegawa, C., Hirata, M., Tsuchiya, T., Yagami, T., Endotoxin contamination in wound dressings made of natural biomaterials (2003) J Biomed Mater Res B Appl Biomater, 66, pp. 347-355; 
(1987) Guideline on validation of the limulus amebocyte lysate test as an end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices, , U.S. Department of Health and Human Services, Public Health Service, Food and Drug Administration; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., Molecular parameters of chitosans depolymerized with the aid of papain (1996) Carbohydr Polym, 29, pp. 63-68; 
Sehgal, D., Vijay, I.K., A method for the high-efficiency of water-soluble carbodiimide-mediated amidation (1994) Anal Biochem, 218, pp. 87-91; 
Leong, K.W., Mao, H.Q., Truong-Le, V.L., Roy, K., Walsh, S.M., August, J.T., DNA-polycation nanospheres as non-viral gene delivery vehicles (1998) J Control Release, 53, pp. 183-193; 
Lee, M., Nah, J.W., Kwon, Y., Koh, J.J., Ko, K.S., Kim, S.W., Water-soluble and low molecular weight chitosan-based plasmid DNA delivery (2001) Pharm Res, 18, pp. 427-431; 
van Kuppeveld, F.J., van der Logt, J.T., Angulo, A.F., van Zoest, M.J., Quint, W.G., Niesters, H.G., Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification (1992) Appl Environ Microbiol, 58, pp. 2606-2615; 
van Kuppeveld, F.J., van der Logt, J.T., Angulo, A.F., van Zoest, M.J., Quint, W.G., Niesters, H.G., Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification (1993) Appl Environ Microbiol, 59, p. 655; 
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays (1983) J Immunol Methods, 65, pp. 55-63; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Godbey, W.T., Ku, K.K., Hirasaki, G.J., Mikos, A.G., Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency (1999) Gene Ther, 6, pp. 1380-1388; 
Pouton, C.W., Lucas, P., Thomas, B.J., Uduehi, A.N., Milroy, D.A., Moss, S.H., Polycation-DNA complexes for gene delivery: a comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids (1998) J Control Release, 53, pp. 289-299; 
Mao, H.Q., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency (2001) J Control Release, 70, pp. 399-421; 
Poole, B., Ohkuma, S., Effect of weak bases on the intralysosomal pH in mouse peritoneal-macrophages (1981) J Cell Biol, 90, pp. 665-669; 
Bowman, E.J., Siebers, A., Altendorf, K., Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells (1988) Proc Natl Acad Sci USA, 85, pp. 7972-7976; 
van Deurs, B., Holm, P.K., Sandvig, K., Inhibition of the vacuolar H+ATPase with bafilomycin reduces delivery of internalized molecules from mature multivesicular endosomes to lysosomes in HEp-2 cells (1996) Eur J Cell Biol, 69, pp. 343-350; 
Merdan, T., Kopecek, J., Kissel, T., Prospects for cationic polymers in gene and oligonucleotide therapy against cancer (2002) Adv Drug Deliv Rev, 54, pp. 715-758; 
Mansouri, S., Lavigne, P., Corsi, K., Benderdour, M., Beaumont, E., Fernandes, J.C., Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy (2004) Eur J Pharm Biopharm, 57, pp. 1-8; 
Erbacher, P., Zou, S.M., Bettinger, T., Steffan, A.M., Remy, J.S., Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability (1998) Pharm Res, 15, pp. 1332-1339; 
Kiang, T., Wen, J., Lim, H.W., Leong, K.W., The effect of the degree of chitosan deacetylation on the efficiency of gene transfection (2004) Biomaterials, 25, pp. 5293-5301; 
Ishii, T., Okahata, Y., Sato, T., Mechanism of cell transfection with plasmid/chitosan complexes (2001) BBA-Biomembranes, 1514, pp. 51-64; 
Lavertu, M., Methot, S., Tran-Khanh, N., Buschmann, M.D., High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation (2006) Biomaterials, 27, pp. 4815-4824; 
de Smedt, S.C., Demeester, J., Hennink, W.E., Cationic polymer based gene delivery systems (2000) Pharm Res, 17, pp. 113-126; 
Kichler, A., Leborgne, C., Coeytaux, E., Danos, O., Polyethylenimine-mediated gene delivery: a mechanistic study (2001) J Gene Med, 3, pp. 135-144; 
Wattiaux, R., Laurent, N., Wattiaux-De Coninck, S., Jadot, M., Endosomes, lysosomes: their implication in gene transfer (2000) Adv Drug Deliv Rev, 41, pp. 201-208; 
Pelkmans, L., Helenius, A., Endocytosis via caveolae (2002) Traffic, 3, pp. 311-320; 
Felgner, P.L., Gadek, T.R., Holm, M., Roman, R., Chan, H.W., Wenz, M., Lipofection-a highly efficient, lipid-mediated DNA-transfection procedure (1987) Proc Natl Acad Sci USA, 84, pp. 7413-7417; 
Conner, S.D., Schmid, S.L., Regulated portals of entry into the cell (2003) Nature, 422, pp. 37-44; 
Koping-Hoggard, M., Tubulekas, I., Guan, H., Edwards, K., Nilsson, M., Varum, K.M., Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo (2001) Gene Ther, 8, pp. 1108-1121; 
Boussif, O., Zanta, M.A., Behr, J.P., Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold (1996) Gene Ther, 3, pp. 1074-1080
},
	month = {2009///},
	pages = {2995 - 3006},
	abstract = {Chitosan was functionalized with imidazole moieties (CHimi) with the aim of improving its buffering capacity and promoting the endosomal escape ability of chitosan-DNA complexes, ultimately increasing their transfection efficiency. 5.6\%, 12.9\% and 22.1\% of the glucosamine residues of chitosan were substituted. Complexes with different molar ratios of primary amines to DNA phosphate anion (N/P) were prepared by a coacervation method. For an N/P \&gt; 3, CHimi polymers are able to complex electrostatically with DNA and condense it into positively charged nanostructures (average size 260 nm and zeta potential +16 mV at pH 5.5). In the concentration range 2.5-100 μg ml},
	keywords = {article, beta galactosidase, Biocompatible Materials, Buffers, Cell Line, cell transport, Chemical structure, chitosan, coacervation, complex formation, controlled study, Cytotoxicity, Diffusion, DNA, Drug Carriers, Drug Compounding, endosome, Gene delivery, Gene transfer, genetic transfection, human, human cell, Humans, imidazole, Kidney, materials testing, nanomaterial, nanoparticle, priority journal, protein expression, proton transport, Tissue engineering, Transfection},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70349162856\&partnerID=40\&md5=fb6672dd0cf06d6ced21eec115b597d2},
	author = {Moreira, C. and Oliveira, H. and Pires, L.R. and Sim{\~o}es, S. and Barbosa, M.A. and P{\^e}go, A.P.}
}
@article {2293,
	title = {Neural cell growth on TiO                    2 anatase nanostructured surfaces
},
	journal = {Thin Solid FilmsThin Solid Films},
	volume = {518},
	year = {2009},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: THSFAdoi: 10.1016/j.tsf.2009.06.048Language of Original Document: EnglishCorrespondence Address: Casa{\~n}-Pastor, N.; Instituto de Ciencias de Materiales de Barcelona, CSIC, Campus la Universidad Autonoma Barcelona, E-08193 Barcelona, Spain; email: nieves@icmab.esReferences: Suzuki, R., Frangos, J.{\'A}., (2000) Clin. Orthop. Relat. Res., 372, p. 280;Suzuki, R., Muyco, J., McKittrick, J., Frangos, J.A., (2003) J. Biomed. Mater. Res. A, 66, p. 396; 
Ulbrich, M.H., Fromherz, P., (2005) Appl. Phys. A., 81, p. 887; 
Topoglidis, E., Lutz, T., Willis, R.L., Barnett, C.J., Cass, A.E., Durrant, J.R., (2000) Faraday Discuss., 116, p. 35; 
Smith, J.R., Walsh, F.C., Clarke, R.L., (1998) J. Appl. Electrochem., 28, p. 1021; 
Dinh, N.N., Oan, N.T., Long, P.D., Bernard, M.N., Hugot-Le Goffb, A., (2003) Thin Solid Films, 423, p. 70; 
(2001) Titanium in Medicine, , Brunette D.M., Tengvall P., Textor M., and Thomsen P. (Eds), Springer-Verlag, Berlin; 
Textor, M., Sittig, C., Frauchiger, V., Tosatti, S., Brunette, D.M., (2001) Titanium in Medicine, 171. , Brunette D.M., Tengvall P., Textor M., and Thomsen P. (Eds), Springer-Verlag, Berlin; 
Contreras, R., Sahlin, H., Frangos, J.{\'A}., (2007) J. Biomed. Mater. Res. A, 80, p. 480; 
Kolbrecka, K., Przyluski, J., (1994) Electrochim. Acta, 39, p. 1591; 
Diebold, U., (2003) Surf. Sci. Rep., 48, p. 53; 
Jeong, B.S., Norton, D.P., Budai, J.D., (2003) Solid-State Electron., 47, p. 2275; 
Moulton, S.E., Barisci, J.N., Bath, A., Stella, R., Wallace, G.C., (2005) Langmuir, 21, p. 316; 
Jackson, D.R., Omanovic, S., Roscoe, S.G., (2005) Langmuir, 16, p. 5449; 
Subramanian, V., Karki, A., Gnanasekar, K.I., Eddy, FP., Rambabu, B., (2006) J. Power Sources, 159, p. 186; 
Carballo-Vila, M., Moreno-Burriel, B., Jurado, J.R., Chinarro, E., Casa{\~n}-Pastor, N., Collazos-Castro, J.E., (2009) J. Biomed. Mater. Res. A, 90, p. 94; 
Brewer, G.J., Torricelli, J.R., Evege, E.K., Price, P.J., (1993) J. Neurosci. Res., 35, p. 567; 
Rajnicek, A.M., Britland, S., McCaig, C.D., (1997) J. Cell Sci., 110, p. 2905; 
Bao, W., Thullberg, M., Zhang, H., Onischenko, A., Str{\"o}mblad, S., (2002) Mol. Cell. Biol., 22, p. 4587; 
Sanjana, N.E., Fuller, S.B., (2004) J. Neurosci. Methods, 136, p. 151; 
Parak, W.J., George, M., Kudera, M., Gaub, H.E., Behrends, J.C., (2001) Eur. Biophys. J., 29, p. 607; 
Dotti, C.G., Sullivan, C.A., Banker, G.A., (1988) J. Neurosci., 8, p. 1454; 
Davenport, R.W., McCaig, C.D., (1993) J. Neurobiology, 24, p. 89; 
Chuckowree, J.A., Vickers, J.C., (2003) J. Neurosci., 23, p. 3715; 
Long, T.C., Saleh, N., Tilton, R.D., Lowry, G.V., Veronesi, B., (2006) Environ. Sci. Technol., 40, p. 4346; 
Zhao, L., Chang, J., Zhai, W., (2005) J. Biomater. Appl., 19, p. 237; 
Swamy, V., Kusnetzov, A., Dubrovinsky, L.S., Caruso, R.A., Shchukin, D.G., Muddle, B.C., (2005) Phys. Rev., B, 71, p. 1; 
Lozano, M.P., Fraxedas, J., (2000) Surf. Interface Anal., 30, p. 623; 
Mart{\'\i}nez-Ferrero, E., Sakatani, Y., Boissi{\`e}re, C., David Grosso, D., Fuertes, A., Fraxedas, J., S{\'a}nchez, C., (2007) Adv. Funct. Mater., 17, p. 3348; 
Roddick-Lanzilotta, A.D., McQuillan, A.J., (1999) J. Colloid Interface Sci., 217, p. 194; 
Kosmulski, M., (2002) Adv. Colloid Interface Sci., 99, p. 255; 
Ikegaya, Y., Itsukaichi-Nishida, Y., Ishihara, M., Tanaka, D., Matsuki, N., (2000) Neuroscience, 97, p. 215; 
Cyster, L.A., Parker, K.G., Parker, T.L., Grant, D.M., (2004) Biomaterials, 25, p. 97; 
Blau, A., Weinl, C., Mack, J., Kienle, S., Jung, G., Ziegler, C., (2001) J. Neurosci. Methods, 112, p. 65; 
Kommireddy, D.S., Sriram, S.M., Lvov, Y.M., Mills, D.K., (2006) Biomaterials, 27, p. 4296; 
Cai, K., Bossert, J., Jandt, K.D., (2006) Colloids Surf., B, 49, p. 136; 
Wang, Y.-X., Robertson, J.L., Spillman Jr., W.B., Claus, R.O., (2004) Pharm. Res., 21, p. 1362; 
Greve, F., Frerker, S., Bittermann, A.G., Burkhardt, C., Hierlemann, A., Hall, H., (2007) Biomaterials, 28, p. 5246; 
Yang, H., Fung, S.-Y., Pritzker, M., Chen, P., (2007) PLoS ONE, 2, pp. e1325; 
Giovambattista, N., Debenedetti, P.G., Rosky, P.J., (2007) J. Phys. Chem. B, 111, p. 9581; 
Onur, M.A., Ta{\c s}, Z., G{\"u}rpnar, A., {\c S}ahin, A., {\c C}ehreli, M.C., (2004) Clin. Oral Implants Res., 15, p. 513; 
Lopez, T., Ortiz-Islas, E., Manjarrez, J., Rodr{\'\i}guez-Reinoso, F., Sep{\'u}lveda, A., Gonzalez, R.D., (2006) Opt. Mater., 29, p. 70
},
	month = {2009///},
	pages = {160 - 170},
	abstract = {Titanium oxides have anti-inflammatory activity and tunable electrochemical properties that make them attractive materials for biomedical applications. This work investigated the compatibility of nanometric coatings of low-temperature phases of TiO                        2 with neurons in 4-day and 10-day cultures, using different cell densities to quantify cell survival and neurite extension. TiO
                        2 films were prepared by sol-gel and thermal treatment (250-450~{\textdegree}C) of hydrolyzed titanium tetra-isopropoxide on electrically conducting or insulating slides. The conducting substrates were not passivated by the nanometric oxide layer and could be used as electrodes. Characterization of the films showed nano-structured TiO
                        2 containing exclusively Ti
                        +4 valence in anatase and amorphous phases. When coated with polylysine, all films permitted good neuron attachment and survival for at least ten days in culture. However, they generally reduced neurite growth compared to cell culture borosilicate glass, with dendrites more affected than axons. The analyses of surface topography, hydrophilicity, charge and chemical composition revealed that TiO
                        2 chemistry was the main factor responsible for neurite inhibition. {\textcopyright} 2009 Elsevier B.V. All rights reserved.
},
	keywords = {Amorphous phasis, Anatase, Anti-inflammatory activity, Biomedical applications, Borosilicate glass, Cell culture, cell density, Cell membranes, cell survival, Chemical compositions, Coatings, Conducting substrates, Conductive films, Electrochemical properties, Gelation, Growth kinetics, Heat treatment, Iso-propoxide, Low temperature phasis, Nano-structured, Nanometrics, Nanostructured surface, Nanostructures, Neural cell, Neural cells, Neurite extension, Neurite growth, Neurites, Neurons, Oxide layer, polylysine, Surface topography, Thermal treatment, TiO, Titanium, Titanium dioxide, Titanium oxide, Titanium oxides},
	isbn = {00406090 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-69649101935\&partnerID=40\&md5=08dd6874d6b2e9bbe7310add08057bc7},
	author = {Collazos-Castro, J.E. and Cruz, A.M. and Carballo-Vila, M. and Lira-Cant{\'u}, M. and Abad, L. and del Pino, {\'A}.P. and Fraxedas, J. and San Juan, A. and Fonseca, C. and P{\^e}go, A.P. and Casa{\~n}-Pastor, N.}
}
@article {2313,
	title = {Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {134},
	year = {2009},
	note = {Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2008.11.007PubMed ID: 19070635Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; Centro de Qu{\'\i}mica da Madeira, Departamento de Qu{\'\i}mica, Universidade da Madeira, Campus Universitario da Penteada, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; calcium, 14092-94-5, 7440-70-2; osteocalcin, 136461-80-8; phosphate, 14066-19-4, 14265-44-2; BMP2 protein, human; Bone Morphogenetic Protein 2; Dendrimers; PAMAM Starburst; Polyamines; beta-Galactosidase, 3.2.1.23References: Dai, J., Rabie, A.B.M., Hagg, U., Xu, R.A., Alternative gene therapy strategies for the repair of craniofacial bone defects (2004) Curr. Gene Ther., 4 (4), pp. 469-485;Gersbach, C.A., Phillips, J.E., Garcia, A.J., Genetic engineering for skeletal regenerative medicine (2007) Annu. Rev. Biomed. Eng., 9, pp. 87-119; 
Kofron, M.D., Laurencin, C.T., Orthopaedic applications of gene therapy (2005) Curr. Gene Ther., 5 (1), pp. 37-61; 
Kofron, M.D., Laurencin, C.T., Bone tissue engineering by gene delivery (2006) Adv. Drug Deliv. Rev., 58 (4), pp. 555-576; 
Luo, J., Sun, M.H., Kang, Q., Peng, Y., Jiang, W., Luu, H.H., Luo, Q., He, T.C., Gene therapy for bone regeneration (2005) Curr. Gene Ther., 5 (2), pp. 167-179; 
Partridge, K.A., Oreffo, R.O., Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies (2004) Tissue Eng., 10 (1-2), pp. 295-307; 
Turgeman, G., Aslan, H., Gazit, Z., Gazit, D., Cell-mediated gene therapy for bone formation and regeneration (2002) Curr. Opin. Mol. Ther., 4 (4), pp. 390-394; 
Chen, Y., Cheung, K.M.C., Kung, H.F., Leong, J.C.Y., Lu, W.W., Luk, K.D.K., In vivo new bone formation by direct transfer of adenoviral-mediated bone morphogenetic protein-4 gene (2002) Biochem. Biophys. Res. Commun., 298 (1), pp. 121-127; 
Lieberman, J.R., Daluiski, A., Stevenson, S., Wu, L., McAllister, P., Lee, Y.P., Kabo, J.M., Witte, O.N., The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats (1999) J. Bone Joint Surg. Am., 81 (7), pp. 905-917; 
Park, J., Ries, J., Gelse, K., Kloss, F., von der Mark, K., Wiltfang, J., Neukam, F.W., Schneider, H., Bone regeneration in critical size defects by cell-mediated BMP-2 gene transfer: a comparison of adenoviral vectors and liposomes (2003) Gene Ther., 10 (13), pp. 1089-1098; 
Riew, K.D., Wright, N.M., Cheng, S.L., Avioli, L.V., Lou, J., Induction of bone formation using a recombinant adenoviral vector carrying the human BMP-2 gene in a rabbit spinal fusion model (1998) Calcif. Tissue Int., 63 (4), pp. 357-360; 
Tsuda, H., Wada, T., Ito, Y., Uchida, H., Dehari, H., Nakamura, K., Sasaki, K., Hamada, H., Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector (2003) Mol. Ther., 7 (3), pp. 354-365; 
De Laporte, L., Cruz Rea, J., Shea, L.D., Design of modular non-viral gene therapy vectors (2006) Biomaterials, 27 (7), pp. 947-954; 
Eliyahu, H., Barenholz, Y., Domb, A.J., Polymers for DNA delivery (2005) Molecules, 10 (1), pp. 34-64; 
Roth, C.M., Sundaram, S., Engineering synthetic vectors for improved DNA delivery: insights from intracellular pathways (2004) Annu. Rev. Biomed. Eng., 6, pp. 397-426; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles (2003) Biomaterials, 24 (7), pp. 1255-1264; 
Hamm, A., Krott, N., Breibach, I., Blindt, R., Bosserhoff, A.K., Efficient transfection method for primary cells (2002) Tissue Eng., 8 (2), pp. 235-245; 
Lakshmipathy, U., Pelacho, B., Sudo, K., Linehan, J.L., Coucouvanis, E., Kaufman, D.S., Verfaillie, C.M., Efficient transfection of embryonic and adult stem cells (2004) Stem Cells, 22 (4), pp. 531-543; 
Kim, S.W., Ogawa, T., Tabata, Y., Nishimura, I., Efficacy and cytotoxicity of cationic-agent-mediated nonviral gene transfer into osteoblasts (2004) J. Biomed. Mater. Res. A, 71 (2), pp. 308-315; 
Dufes, C., Uchegbu, I.F., Schatzlein, A.G., Dendrimers in gene delivery (2005) Adv. Drug Deliv. Rev., 57 (15), pp. 2177-2202; 
Guillot-Nieckowski, M., Eisler, S., Diederich, F., Dendritic vectors for gene transfection (2007) New J. Chem., 31 (7), pp. 1111-1127; 
Svenson, S., Tomalia, D.A., Dendrimers in biomedical applications-reflections on the field (2005) Adv. Drug Deliv. Rev., 57 (15), pp. 2106-2129; 
Haensler, J., Szoka, F.C., Polyamidoamine cascade polymers mediate efficient transfection of cells in culture (1993) Bioconjug. Chem., 4 (5), pp. 372-379; 
Choi, J.S., Nam, K., Park, J., Kim, J.B., Lee, J.K., Park, J., Enhanced transfection efficiency of PAMAM dendrimer by surface modification with l-arginine (2004) J. Control. Release, 99 (3), pp. 445-456; 
Kono, K., Akiyama, H., Takahashi, T., Takagishi, T., Harada, A., Transfection activity of polyamidoamine dendrimers having hydrophobic amino acid residues in the periphery (2005) Bioconjug. Chem., 16 (1), pp. 208-214; 
KukowskaLatallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., Baker, J.R., Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers (1996) Proc. Natl. Acad. Sci. U. S. A., 93 (10), pp. 4897-4902; 
Gebhart, C.L., Kabanov, A.V., Evaluation of polyplexes as gene transfer agents (2001) J. Control. Release, 73 (2), pp. 401-416; 
Aubin, J.E., Osteoprogenitor cell frequency in rat bone marrow stromal populations: role for heterotypic cell-cell interactions in osteoblast differentiation (1999) J. Cell. Biochem., 72 (3), pp. 396-410; 
Lennon, D.P., Haynesworth, S.E., Young, R.G., Dennis, J.E., Caplan, A.I., A chemically-defined medium supports in-vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stem-cells (1995) Exp. Cell Res., 219 (1), pp. 211-222; 
Bielinska, A.U., Chen, C.L., Johnson, J., Baker, J.R., DNA complexing with polyamidoamine dendrimers: implications for transfection (1999) Bioconjug. Chem., 10 (5), pp. 843-850; 
Eichman, J.D., Bielinska, A.U., Kukowska-Latallo, J.F., Baker Jr., J.R., The use of PAMAM dendrimers in the efficient transfer of genetic material into cells (2000) Pharm. Sci. Technol. Today, 7, pp. 232-245; 
Haleem-Smith, H., Derfoul, A., Okafor, C., Tuli, R., Olsen, D., Hall, D.J., Tuan, R.S., Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro (2005) Mol. Biotechnol., 30 (1), pp. 9-19; 
Fischer, D., Li, Y.X., Ahlemeyer, B., Krieglstein, J., Kissel, T., In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis (2003) Biomaterials, 24 (7), pp. 1121-1131; 
Jevprasesphant, R., Penny, J., Jalal, R., Attwood, D., McKeown, N.B., D{\textquoteright}Emanuele, A., The influence of surface modification on the cytotoxicity of PAMAM dendrimers (2003) Int. J. Pharm., 252 (1-2), pp. 263-266; 
Wilke, M., Fortunati, E., vandenBroek, M., Hoogeveen, A.T., Scholte, B.J., Efficacy of a peptide-based gene delivery system depends on mitotic activity (1996) Gene Ther., 3 (12), pp. 1133-1142; 
Xie, Y.B., Yang, S.T., Kniss, D.A., Three-dimensional cell-scaffold constructs promote efficient gene transfection: implications for cell-based gene therapy (2001) Tissue Eng., 7 (5), pp. 585-598; 
Yamagiwa, H., Endo, N., Tokunaga, K., Hayami, T., Hatano, H., Takahashi, H.E., In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2 (2001) J. Bone Miner. Metab., 19 (1), pp. 20-28; 
Chaudhari, A., Ron, E., Rethman, M.P., Recombinant human bone morphogenetic protein-2 stimulates differentiation in primary cultures of fetal rat calvarial osteoblasts (1997) Mol. Cell. Biochem., 167 (1-2), pp. 31-39; 
Hay, E., Hott, M., Graulet, A.M., Lomri, A., Marie, P.J., Effects of bone morphogenetic protein-2 on human neonatal calvaria cell differentiation (1999) J. Cell. Biochem., 72 (1), pp. 81-93; 
Yamaguchi, A., Regulation of differentiation pathway of skeletal mesenchymal cells in cell-lines by transforming growth-factor-beta superfamily (1995) Semin. Cell Biol., 6 (3), pp. 165-173; 
Yamaguchi, A., Ishizuya, T., Kintou, N., Wada, Y., Katagiri, T., Wozney, J.M., Rosen, V., Yoshiki, S., Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3-G2/PA6 (1996) Biochem. Biophys. Res. Commun., 220 (2), pp. 366-371; 
Baltzer, A.W.A., Lattermann, C., Whalen, J.D., Wooley, P., Weiss, K., Grimm, M., Ghivizzani, S.C., Evans, C.H., Genetic enhancement of fracture repair: healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene (2000) Gene Ther., 7 (9), pp. 734-739; 
Wang, E.A., Rosen, V., Dalessandro, J.S., Bauduy, M., Cordes, P., Harada, T., Israel, D.I., Wozney, J.M., Recombinant human bone morphogenetic protein induces bone-formation (1990) Proc. Natl. Acad. Sci. U. S. A., 87 (6), pp. 2220-2224; 
Chuang, C.K., Sung, L.Y., Hwang, S.M., Lo, W.H., Chen, H.C., Hu, Y.C., Baculovirus as a new gene delivery vector for stem cell engineering and bone tissue engineering (2007) Gene Ther., 14 (19), pp. 1417-1424; 
Aslan, H., Zilberman, Y., Arbeli, V., Sheyn, D., Matan, Y., Liebergall, M., Li, J.Z., Gazit, Z., Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration (2006) Tissue Eng., 12 (4), pp. 877-889; 
Noel, D., Gazit, D., Bouquet, C., Apparailly, F., Bony, C., Plence, P., Millet, V., Jorgensen, C., Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells (2003) Stem Cells, 22 (1), pp. 74-85
},
	month = {2009///},
	pages = {141 - 148},
	abstract = {This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a β-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {alkaline phosphatase, amine, Amines, animal cell, Animals, article, beta galactosidase, beta-Galactosidase, Bone, Bone morphogenetic protein, bone morphogenetic protein 2, Bone Regeneration, Calcium, Cell culture, cell differentiation, Cell Line, cell lineage, cell survival, Cells, Cells, Cultured, controlled study, dendrimer, Dendrimers, embryo, enzyme activity, Flowcharting, gene expression, Gene transfer, Gene Transfer Techniques, gene vector, Genes, Reporter, genetic transfection, human, human cell, Humans, in vitro study, Kidney, male, Mesenchymal stem cell, Mesenchymal Stem Cells, nonhuman, Nonviral gene delivery, nonviral gene delivery system, Nucleic acids, osteoblast, osteocalcin, Osteogenesis, PAMAM dendrimers, phosphate, plasmid, Plasmids, polyamidoamine, Polyamines, priority journal, protein secretion, Proteins, qualitative analysis, quantitative analysis, rat, Rats, Rats, Wistar, reporter gene, staining},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-60649096454\&partnerID=40\&md5=90df49394a581738b41bf29bad341ae8},
	author = {Santos, J.L. and Oramas, E. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
