



@article {2576,
	title = {Miniaturization and application of the MTT assay to evaluate metabolic activity of protozoa in the presence of toxicants},
	journal = {Journal of Basic MicrobiologyJ. Basic Microbiol.},
	volume = {39},
	year = {1999},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JBMIEdoi: 10.1002/(SICI)1521-4028(199905)39:2<103::AID-JOBM103>3.0.CO;2-DPubMed ID: 10335603Language of Original Document: EnglishCorrespondence Address: Lima, N.; Centro de Engenharia Biologica, Universidade do Minho, 4700 Braga, Portugal; email: nelson@iec.uminho.ptChemicals/CAS: Copper, 7440-50-8; Cycloheximide, 66-81-9; Octoxynol, 9002-93-1; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1; Zinc, 7440-66-6References: Berridge, M.V., Tan, S., Characterization of the cellular reduction of using 3-[4,5-dimethyl-thiazol-2-y1]-2,5-diphenyl-tetrazolium bromide-MTT: Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction (1993) Arch. Biochem. Biophysics, 303, pp. 474-482;Berridge, M.V., Tan, S., McCoy, K.D., Wang, R., The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts (1996) Biochemica, 4, pp. 15-20; 
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},
	month = {1999///},
	pages = {103 - 108},
	abstract = {This paper describes a critical evaluation of a miniaturised colorimetric assay, using MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) reduction, applied to protozoan viability testing. The toxic substances used were copper, zinc, Triton X-100 (a membrane surfactant) and cycloheximide (an inhibitor of the protein synthesis). The viability assay of the ciliate protozoan Tetrahymena pyriformis was optimised in terms of MTT concentration and incubation time. Since protozoa are non adherent cells the MTT assay was modified in order to maintain the medium in the well. MTT proved to be effective in the measurement of Tetrahymena pyriformis viability. Four hours of MTT incubation followed by 30 minutes of incubation with DMSO were found to be the best incubation times for optical density reading. Furthermore, 10 mg/ml of MTT solution was the concentration that gave higher values of optical densities with minor medium interference.},
	keywords = {Animals, Ciliophora, Colorimetry, Copper, Cycloheximide, MTT assay, Octoxynol, Protozoa, Tetrahymena pyriformis, Tetrazolium Salts, Thiazoles, Zinc},
	isbn = {0233111X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032910642\&partnerID=40\&md5=81e222339d4ee5c21619858027047e83},
	author = {Dias, N. and Nicolau, A. and Carvalho, G.S. and Mota, M. and Lima, N.}
}
