



@article {2856,
	title = {Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration - In vitro study},
	journal = {Journal of Biomedical Materials Research - Part B Applied Biomaterials},
	volume = {101 B},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {762 - 769},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84878874447\&partnerID=40\&md5=edd0491ee0c1e8faf857a483e14efaf9},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Carvalho, A. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
@article {2178,
	title = {Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration},
	journal = {Dental MaterialsDent. Mater.},
	volume = {28},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: DEMAEdoi: 10.1016/j.dental.2012.09.002Language of Original Document: EnglishCorrespondence Address: Carvalho, A.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: angela.carvalho@ineb.up.ptReferences: Neumann, A., Biomaterials for craniofacial reconstruction (2009) Laryngo- Rhino- Otologie, 88 (SUPPL. 1), pp. 48-S63;Klinge, B., Hultin, M., Berglundh, T., Peri-implantitis (2005) Dental Clinics of North America, 49 (3 SPEC. ISSUE), pp. 661-676. , DOI 10.1016/j.cden.2005.03.007, PII S0011853205000236; 
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},
	month = {2012///},
	pages = {1250 - 1260},
	abstract = {Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO                        2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 
                        2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 
                        2 surfaces with 0\%, 1\% and 5\% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 
                        2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. {\textcopyright} 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
},
	keywords = {Adhesion, AFM, Aggregates, Bacteria, Bacterial adhesion, Bioactive nanoparticles, Biological characterization, Biological materials, Biological response, Biomaterials, Cell culture, Contact angle, Dental prostheses, Flowcharting, FTIR/ATR, Guided tissue regeneration, Lithography, Mesenchymal stem cell, Micro patterning, Micro-texture, Microaggregates, Micropatterned, Micropatterned surface, Micropatterning, Nano-HA, Nano-hydroxyapatite, S. mutans, SEM/EDS, Silica, Silica thin films, Soft-lithography, Sol-gel, Sol-gel process, Sol-gels, Streptococcus mutans, Surface modification techniques, Surfaces, Thin films},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867841771\&partnerID=40\&md5=f33477063f16e99361687470c94d7289},
	author = {Carvalho, A. and Pelaez-Vargas, A. and Gallego-Perez, D. and Grenho, L. and Fernandes, M.H. and De Aza, A.H. and Ferraz, M.P. and Hansford, D.J. and Monteiro, F.J.}
}
