



@article {3265,
	title = {Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation},
	journal = {Journal of Biomaterials Applications},
	volume = {28},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1325 - 1335},
	abstract = {Nanohydroxyapatite (nanoHA), due to its chemical properties, has appeared as an exceptionally promising bioceramic to be used as bone regeneration material. Staphylococcus epidermidis have emerged as major nosocomial pathogens associated with infections of implanted medical devices. In this work, the purpose was to study the influence of the nanoHA surface characteristics on S. epidermidis RP62A biofilm formation. Therefore, two different initial inoculum concentrations (Ci) were used in order to check if these would affect the biofilm formed on the nanoHA surfaces. Biofilm formation was followed by the enumeration of cultivable cells and by scanning electron microscopy. Surface topography, contact angle, total surface area and porosimetry of the biomaterials were studied and correlated with the biofilm data. The surface of nanoHA sintered at 830 (nanoHA830) showed to be more resistant to S. epidermidis attachment and accumulation than that of nanoHA sintered at 1000 (nanoHA1000). The biofilm formed on nanoHA830 presented differences in terms of structure, surface coverage and EPS production when compared to the one formed on nanoHA1000 surface. It was observed that topography and surface area of nanoHA surfaces had influence on the bacterial attachment and accumulation. Ci influenced bacteria attachment and accumulation on nanoHA surfaces over time. The choice of the initial inoculum concentration was relevant proving to have an effect on the extent of adherence thus being a critical point for human health if these materials are used in implantable devices. This study showed that the initial inoculum concentration and surface material properties determine the rate of microbial attachment to substrata and consequently are related to biofilm-associated infections in biomaterials. {\textcopyright} The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.},
	keywords = {biofilm formation, biomaterial roughness, biomaterial surface area, Inoculum concentration, Nanohydroxyapatite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84890640286\&partnerID=40\&md5=0f7d5eb37db34b3d0a1e4fb334846340},
	author = {Barros, J. and Grenho, L. and Manuel, C.M. and Ferreira, C. and Melo, L. and Nunes, O.C. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {3292,
	title = {Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications},
	journal = {Science and Technology of Advanced Materials},
	volume = {15},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	abstract = {Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol-gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior. {\textcopyright} 2014 National Institute for Materials Science.},
	keywords = {bacteria adhesion, Biomaterials, hemolysis, human dermal microvascular endothelial cells (HDMECs), human gingival fibroblasts (HGFs), Micropatterning, Soft lithography},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84898687729\&partnerID=40\&md5=f252b87aeb163775547158cad0cf658b},
	author = {Laranjeira, M.S. and Carvalho, {\^A}. and Pelaez-Vargas, A. and Hansford, D. and Ferraz, M.P. and Coimbra, S. and Costa, E. and Santos-Silva, A. and Fernandes, M.H. and Monteiro, F.J.}
}
@article {2205,
	title = {Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa onto nanohydroxyapatite as a bone regeneration material},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {100 A},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.34139PubMed ID: 22489063Language of Original Document: EnglishCorrespondence Address: Grenho, L.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: liliana.grenho@ineb.up.ptChemicals/CAS: Biocompatible Materials; Durapatite, 1306-06-5References: Darley, E.S.R., MacGowan, A.P., Antibiotic treatment of Gram-positive bone and joint infections (2004) J Antimicrob Chemother, 53, pp. 928-935;Charville, G.W., Hetrick, E.M., Geer, C.B., Schoenfisch, M.H., Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release (2008) Biomaterials, 29, pp. 4039-4044; 
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},
	month = {2012///},
	pages = {1823 - 1830},
	abstract = {In orthopedics due to the enormous number of surgical procedures involving invasive implant biomaterials, infections have a huge impact in terms of morbidity, mortality, and medical costs. In this study the initial adhesion of several strains namely Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa, to nanohydroxyapatite, previously heat-treated at 725{\textdegree}C and 1000{\textdegree}C was assessed. Adherent cells were evaluated by scanning electron microscopy and quantified by confocal laser scanning microscopy and as colony forming units after being released by sonication. The wettability and roughness of samples surfaces were assessed by contact angle measurements and atomic force microscopy, respectively. Nanohydroxyapatite heat-treated at 1000{\textdegree}C appeared to be more resistant to bacterial adhesion, over time, in five of the six tested strains while the clinical strains isolated from orthopedic infections presented superior ability to adhere, as well as better capacity to produce slime. The increase in materials sintering temperature resulted in increased hydrophobicity and roughness; however, other surface features such as the decrease in surface area and on porosity as well as the decrease on zeta potential may be the aspects that contributed to a lower bacterial adhesion on the materials sintered at 1000{\textdegree}C. {\textcopyright} 2012 Wiley Periodicals, Inc.},
	keywords = {Adherent cells, Adhesion, article, Atomic force microscopy, Bacteria, Bacterial adhesion, bacterium adherence, Biocompatible Materials, Biological materials, biomaterial, Biomaterials, Bone, bone infection, Bone Regeneration, Bone regeneration materials, Clinical strains, Colony Count, Microbial, colony forming unit, Colony forming units, confocal laser microscopy, Confocal laser scanning microscopy, confocal microscopy, Contact angle, Durapatite, hydrophobicity, Microscopy, Confocal, Microscopy, Electron, Scanning, Nano-hydroxyapatite, Nanohydroxyapatite, nanoparticles, nonhuman, porosity, Pseudomonas aeruginosa, Scanning electron microscopy, Sintering, Sintering temperatures, Staphylococcus aureus, Staphylococcus epidermidis, Surface area, Surface feature, surface property, Surface roughness, Surgical procedures, temperature, ultrasound, unclassified drug, wettability, Zeta potential},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861570738\&partnerID=40\&md5=84186d33133ff6c5280db80ec9fcb12d},
	author = {Grenho, L. and Manso, M.C. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {2178,
	title = {Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration},
	journal = {Dental MaterialsDent. Mater.},
	volume = {28},
	year = {2012},
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},
	month = {2012///},
	pages = {1250 - 1260},
	abstract = {Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO                        2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 
                        2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 
                        2 surfaces with 0\%, 1\% and 5\% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 
                        2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. {\textcopyright} 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
},
	keywords = {Adhesion, AFM, Aggregates, Bacteria, Bacterial adhesion, Bioactive nanoparticles, Biological characterization, Biological materials, Biological response, Biomaterials, Cell culture, Contact angle, Dental prostheses, Flowcharting, FTIR/ATR, Guided tissue regeneration, Lithography, Mesenchymal stem cell, Micro patterning, Micro-texture, Microaggregates, Micropatterned, Micropatterned surface, Micropatterning, Nano-HA, Nano-hydroxyapatite, S. mutans, SEM/EDS, Silica, Silica thin films, Soft-lithography, Sol-gel, Sol-gel process, Sol-gels, Streptococcus mutans, Surface modification techniques, Surfaces, Thin films},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84867841771\&partnerID=40\&md5=f33477063f16e99361687470c94d7289},
	author = {Carvalho, A. and Pelaez-Vargas, A. and Gallego-Perez, D. and Grenho, L. and Fernandes, M.H. and De Aza, A.H. and Ferraz, M.P. and Hansford, D.J. and Monteiro, F.J.}
}
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	title = {Staphylococcus aureus and Staphylococcus epidermidis adhesion to nanohydroxyapatite in the presence of model proteins},
	journal = {Biomedical Materials (Bristol)Biomed. Mater.},
	volume = {7},
	year = {2012},
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},
	month = {2012///},
	abstract = {Bacterial infections can have adverse effects on the efficacy, lifetime, and safety of an implanted device. The aim of this study was to investigate the initial adhesion of several strains, namely S. aureus and S. epidermidis, on two distinct types of nanohydroxyapatite (nanoHA), sintered at 725{\textdegree}C and 1000{\textdegree}C. A comparison was also made with nanohydroxyapatite having adsorbed fetal bovine serum (FBS), human fibronectin (FN) and human serum albumin (HSA). Adhered bacterial cells were examined by scanning electron microscopy and quantified as colony forming units after being released by sonication. The wettability of the sample surface with and without adsorbed protein was assessed by contact-angle measurements. NanoHA sintered at 1000{\textdegree}C showed lower bacterial adhesion than this heat-treated at 725{\textdegree}C. Adsorption of FBS onto the nanoHA surface caused a decrease in the adhesion of all strains on both materials. The bacterial adhesion patterns in the presence of FN were different for both nanoHA substrates; the adherence of the bacterial strains, except for the clinical strain of S.epidermidis, was significantly higher on nanoHA 1000 in comparison to nanoHA 1000 without protein and the bacterial adhesion on the FN-coated nanoHA 725 was lower in comparison to the bare nanoHA 725. The effect of HSA on bacterial adhesion was concentration and bacterial strain dependent. {\textcopyright} 2012 IOP Publishing Ltd.},
	keywords = {Adhesion, Adsorption, Adverse effect, article, Bacteria, Bacterial adhesion, bacterial cell, Bacterial cells, Bacterial infections, bacterial strain, Bacterial strains, bacterium adherence, Clinical strains, colony forming unit, Colony forming units, Contact angle, Contact-angle measurements, controlled study, Enzyme inhibition, Epidermidis, Fetal bovine serum, Fibronectin, heating, Human fibronectin, human serum albumin, Human serum albumins, hydroxyapatite, Implanted device, materials testing, Model proteins, Nano-HA, Nano-hydroxyapatite, Nanohydroxyapatite, nanoparticle, nonhuman, Proteins, S. aureus, Sample surface, Scanning electron microscopy, serum, Sintering, Staphylococcus aureus, Staphylococcus epidermidis, surface property, ultrasound, unclassified drug, wettability},
	isbn = {17486041 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864391456\&partnerID=40\&md5=9170b87ade5223df5146d21981d073ef},
	author = {Ribeiro, M. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {2269,
	title = {Heparinized hydroxyapatite/collagen three-dimensional scaffolds for tissue engineering},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {21},
	year = {2010},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-010-4097-2PubMed ID: 20596760Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: collagen, 9007-34-5; cyanamide, 151-51-9, 420-04-2; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; hydroxyapatite, 1306-06-5, 51198-94-8; n hydroxysuccinimide, 6066-82-6; trinitrobenzenesulfonic acid, 16655-63-3, 2508-19-2; Biocompatible Materials; Bone Substitutes; Collagen, 9007-34-5; Cross-Linking Reagents; Durapatite, 1306-06-5; Heparin, 9005-49-6; PolymersReferences: Rose, F.R., Cyster, L.A., Grant, D.M., Scotchford, C.A., Howdle, S.M., Shakesheff, K.M., In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel (2004) Biomaterials, 25 (24), pp. 5507-5514. , DOI 10.1016/j.biomaterials.2004.01.012, PII S0142961204000134;Hong, Z., Zhang, P., He, C., Qiu, X., Liu, A., Chen, L., Chen, X., Jing, X., Nano-composite of poly(L-lactide) and surface grafted hydroxyapatite: Mechanical properties and biocompatibility (2005) Biomaterials, 26 (32), pp. 6296-6304. , DOI 10.1016/j.biomaterials.2005.04.018, PII S0142961205003297; 
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Teixeira, S., Oliveira, S., Ferraz, M., Monteiro, F.J., Three dimensional macroporous calcium phosphates scaffolds for bone tissue engineering (2008) Key Eng Mater Trans Tech, pp. 947-50; 
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Teixeira, S., Rodriguez, M.A., Pena, P., De Aza, A.H., De Aza, S., Ferraz, M.P., Monteiro, F.J., Physical characterization of hydroxyapatite porous scaffolds for tissue engineering (2009) Mater Sci Eng C, 29 (5), pp. 1510-1514. , 10.1016/j.msec.2008.09.052 1:CAS:528:DC\%2BD1MXmtVarurg\%3D
},
	month = {2010///},
	pages = {2385 - 2392},
	abstract = {Currently, in bone tissue engineering research, the development of appropriate biomaterials for the regeneration of bony tissues is a major concern. Bone tissue is composed of a structural protein, collagen type I, on which calcium phosphate crystals are enclosed. For tissue engineering, one of the most applied strategies consists on the development and application of three dimensional porous scaffolds with similar composition to the bone. In this way, they can provide a physical support for cell attachment, proliferation, nutrient transport and new bone tissue infiltration. Hydroxyapatite is a calcium phosphate with a similar composition of bone and widely applied in several medical/dentistry fields. Therefore, in this study, hydroxyapatite three dimensional porous scaffolds were produced using the polymer replication method. Next, the porous scaffolds were homogeneously coated with a film of collagen type I by applying vacuum force. Yet, due to collagen degradability properties, it was necessary to perform an adequate crosslinking method. As a result, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was employed as an efficient and non-toxic crosslinking method in this research. The composites were characterized by means of SEM, DSC and TNBS. Furthermore, heparin was incorporated in order to accomplish sustained delivery of a growth factor of interest namely, bone morphogenetic proteins (BMP-2). BMP-2 binding and release of non-heparinized and heparinized scaffolds was evaluated at specific time points. The incorporation of heparin leads to a reduced initial burst phase when compared to the non heparinized materials. The results show a beneficial effect with the incorporation of heparin and its potential as a localized drug delivery system for the sustained release of growth factors. {\textcopyright} 2010 Springer Science+Business Media, LLC.},
	keywords = {Achilles Tendon, Animals, Apatite, article, Beneficial effects, Binding and release, Biocompatible Materials, Biological materials, Biomaterials, Bone, Bone and Bones, bone morphogenetic protein 2, Bone morphogenetic proteins, Bone Substitutes, bone tissue, Bone tissue engineering, Bony tissue, Calcium, calcium phosphate, Calcium phosphate crystals, Cattle, Cell attachments, Collagen, collagen degradation, collagen type 1, Collagen type I, controlled study, cross linking, Cross-Linking Reagents, Crosslinking, cyanamide, Degradability, differential scanning calorimetry, Drug delivery, drug delivery system, Durapatite, Grafts, Growth factor, heparin, Hydrogels, hydroxyapatite, Microscopy, Electron, Scanning, n (3 dimethyllaminopropyl) n{\textquoteright} ethylcarbodiimide, n hydroxysuccinimide, N-hydroxysuccinimide, Nutrient transport, Peptides, polymer, Polymer replication, Polymers, Polysaccharides, porosity, Porous scaffold, priority journal, protein binding, Scaffolds, Scanning electron microscopy, SEM, Specific time, Structural proteins, Sustained release, Three dimensional, Three-dimensional porous scaffolds, Three-dimensional scaffolds, Tissue engineering, tissue scaffold, Tissue Scaffolds, trinitrobenzenesulfonic acid, unclassified drug, vacuum, Vacuum force},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956420058\&partnerID=40\&md5=a723fdb7ea856a5286080a1a4aab82a2},
	author = {Teixeira, S. and Yang, L. and Dijkstra, P.J. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2276,
	title = {In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {93},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32532PubMed ID: 19591232Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Ceramics; Durapatite, 1306-06-5References: Jones, A.C., Milthorpe, B., Averdunk, H., Limaye, A., Senden, T.J., Sakellariou, A., Sheppard, A.P., Dwdw, H., Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging (2004) Biomaterials, 25, pp. 4947-4954;Aizawa, M., Shinoda, H., Uchida, H., Itatani, K., Okada, I., Matsumoto, M., Toyama, Y., Development and biological evaluation of apatite fibre scaffolds with large pore size and high porosity for bone regeneration (2003) Bioceramics, 240-242, pp. 647-650; 
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Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci Mater Med, 19, pp. 855-859; 
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},
	month = {2010///},
	pages = {567 - 575},
	abstract = {During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA based porous scaffolds with collagen type I. The results indicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds. {\textcopyright} 2009 Wiley Periodicals, Inc.},
	keywords = {animal cell, animal experiment, animal model, Animals, article, Biocompatibility, Biocompatible Materials, Biodegradation, Bone, Bone and Bones, Bone formation, bone tissue, Calcium, Calcium alloys, calcium phosphate, Calcium phosphates, Cell Count, Cell culture, Cell Culture Techniques, Cells, Cultured, cellular distribution, Ceramic materials, ceramics, Collagen, collagen type 1, Durapatite, Energy dispersive spectroscopy, Flowcharting, Fourier transform infrared spectroscopy, Humans, hydroxyapatite, Hydroxylation, in vitro study, In vivo bone formation, in vivo study, In-vivo, infrared spectroscopy, Macroporous, Macroporous scaffolds, male, materials testing, Mesenchymal stem cell, Mesenchymal Stem Cells, Mice, Microscopy, Electron, Scanning, nonhuman, ossification, Osteogenesis, porous polymer, rat, Rats, Rats, Wistar, Scaffolds, Scanning electron microscopy, Spectroscopy, Fourier Transform Infrared, Stem cells, Tissue engineering, tissue scaffold, Tissue Scaffolds},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77950190285\&partnerID=40\&md5=612e26b56020935c7b0b1c564bffa89a},
	author = {Teixeira, S. and Fernandes, H. and Leusink, A. and Van Blitterswijk, C. and Ferraz, M.P. and Monteiro, F.J. and De Boer, J.}
}
@article {2252,
	title = {Proliferation and mineralization of bone marrow cells cultured on macroporous hydroxyapatite scaffolds functionalized with collagen type I for bone tissue regeneration},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32600Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Divis{\~a}o de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: 1 (3 dimethylaminopropyl) 3 ethylcarbodiimide, 1892-57-5, 25952-53-8, 7084-11-9; DNA, 9007-49-2; hydroxyapatite, 1306-06-5, 51198-94-8; methylene blue, 61-73-4; n hydroxysuccinimide, 6066-82-6; Collagen Type I; Cross-Linking Reagents; DNA, 9007-49-2; Durapatite, 1306-06-5; Methylene Blue, 61-73-4Manufacturers: SigmarecticelReferences: Nair, M.B., Suresh Babu, S., Varma, H.K., John, A., A triphasic ceramic-coated porous hydroxyapatite for tissue engineering application (2008) Acta Biomaterialia, 4 (1), pp. 173-181. , DOI 10.1016/j.actbio.2007.07.004, PII S174270610700102X;Navarro, M., Ginebra, M.P., Planell, J.A., Zeppetelli, S., Ambrosio, L., Development and cell response of a new biodegradable composite scaffold for guided bone regeneration (2004) Journal of Materials Science: Materials in Medicine, 15 (4), pp. 419-422. , DOI 10.1023/B:JMSM.0000021113.88702.9d; 
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Friess, W., Collagen - Biomaterial for drug delivery (1998) Eur J Pharm Biopharm, 45, pp. 113-136; 
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Yoshioka, S.A., Goissis, G., Thermal and spectrophotometric studies of new crosslinking method for collagen matrix with glutaraldehyde acetals (2008) Journal of Materials Science: Materials in Medicine, 19 (3), pp. 1215-1223. , DOI 10.1007/s10856-007-0151-0; 
Wissink, M.J., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Binding and release of basic fibroblast growth factor from heparinized collagen matrices (2001) Biomaterials, 22, pp. 2291-2299; 
Wissink, M.J., Beernink, R., Pieper, J.S., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Immobilization of heparin to EDC/NHS-crosslinked collagen. Characterization and in vitro evaluation (2001) Biomaterials, 22, pp. 151-163; 
Wissink, M.J., Beernink, R., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices (2000) J Control Release, 64, pp. 103-114; 
Wissink, M.J., Beernink, R., Scharenborg, N.M., Poot, A.A., Engbers, G.H., Beugeling, T., Van Aken, W.G., Feijen, J., Endothelial cell seeding of (heparinized) collagen matrices: Effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors (2000) J Control Release, 67, pp. 141-155; 
Wissink, M.J.B., Van Luyn, M.J.A., Beernink, R., Dijk, F., Poot, A.A., Engbers, G.H.M., Beugeling, T., Feijen, J., Endothelial cell seeding on crosslinked collagen: Effects of crosslinking on endothelial cell proliferation and functional parameters (2000) Thrombosis and Haemostasis, 84 (2), pp. 325-331; 
Teixeira, S., Oliveira{\l}, S., Ferraz{\l}, M.P., Monteiro, F.J., Three dimensional macroporous calcium phosphate scaffolds for bone tissue engineering (2008) Key Eng Mater, 361-363, pp. 947-950; 
Teixeira, S., Rodriguez, M., Pena, P., De Aza, A., De Aza, S., Ferraz, M., Monteiro, F., Physical characterization of hydroxyapatite porous scaffolds for tissue engineering (2009) Mater Sci Eng: C, 29, pp. 1510-1514
},
	month = {2010///},
	pages = {1 - 8},
	abstract = {This study concerns the preparation and in vitro characterization of functionalized hydroxyapatite (HA) porous scaffolds, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite scaffolds were prepared using the polymer replication method, and, after being submitted to a specific sintering cycle, collagen Type I was incorporated on the surface. After the coating procedure, collagen was crosslinked using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) conjugation method. In this study, hydroxyapatite scaffolds with uncrosslinked and cross-linked Type I collagen were evaluated. Cell morphology and deposition of extracellular matrix were assessed by scanning electron microscopy, whereas cell distribution was visualized by means of methylene blue staining. MTS and total DNA quantification assays were used to evaluate the viability and proliferation of human bone marrow cells cultured on all the materials for 28 days. Results showed that the cells were able to adhere, proliferate, and form a mineralized matrix on the surface of all the materials. Furthermore, the cells were able to spread from one pore to another and form cell clusters. The results show that these scaffolds are good candidates to serve as drug delivery vehicles and for tissue engineering purposes. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {1 (3 dimethylaminopropyl) 3 ethylcarbodiimide, adult, Animal cell culture, Apatite, article, Automobile exhibitions, Bone, bone marrow cell, Bone Marrow Cells, bone matrix, bone mineralization, Bone Regeneration, bone tissue, Bone tissue regeneration, Calcification, Physiologic, cell adhesion, Cell clusters, Cell culture, Cell distribution, Cell morphology, cell proliferation, cell structure, cell viability, Cells, Cultured, cellular distribution, Ceramic materials, chemistry, Coating procedures, Collagen, collagen type 1, Collagen type I, conjugation, controlled study, cross linking, cross linking reagent, Cross-Linking Reagents, Crosslinked, cytology, Cytotoxicity, DNA, DNA quantification, drug conjugation, Drug delivery, drug delivery system, Drug delivery vehicles, drug effect, Drug-delivery systems, Durapatite, Extracellular matrices, extracellular matrix, Functionalized, human, Human bone marrow cells, human cell, Humans, hydroxyapatite, in vitro study, In-vitro, Macroporous, Macroporous ceramics, male, matrix, metabolism, methylene blue, Methylene blue staining, Microscopy, Electron, Scanning, molecular scaffold, n hydroxysuccinimide, N-hydroxysuccinimide, polymer, Polymer replication, porosity, Porous scaffold, Scaffolds, Scanning electron microscopy, Sintering, Sintering cycles, spectrometry, Spectrometry, X-Ray Emission, Tissue engineering, tissue scaffold, Tissue Scaffolds, Type I collagen, Young Adult},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956483928\&partnerID=40\&md5=de85a8bfe5af88cbc45e80302ab4dfd4},
	author = {Teixeira, S. and Fernandes, M.H. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2306,
	title = {Cationic liposome-DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cells},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.02.019PubMed ID: 19332382Language of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; CEBIMED, Faculdade de Ci{\^e}ncias da Sa{\'u}, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal; email: mpferraz@ufp.ptChemicals/CAS: 1,2 dioleoyl 3 trimethylammoniopropane, 144189-73-1; DNA, 9007-49-2; transferrin, 82030-93-1; DNA, 9007-49-2; LiposomesReferences: Luo, J., Sun, M.H., Kang, Q., Peng, Y., Jiang, W., Luu, H.H., Gene therapy for bone regeneration (2005) Curr Gene Ther, 5 (2), pp. 167-179;Oreffo, R.O.C., Triffitt, J.T., Future potentials for using osteogenic stem cells and biomaterials in orthopedics (1999) Bone, 25 (2 SUPPL. 1), pp. 5S-9S; 
Laurencin, C.T., Attawia, M.A., Lu, L.Q., Borden, M.D., Lu, H.H., Gorum, W.J., Poly(lactide-co-glycolide)/hydroxyapatite delivery of BMP-2-producing cells: a regional gene therapy approach to bone regeneration (2001) Biomaterials, 22 (11), pp. 1271-1277; 
Katz, J.L., (1996) Applications of materials in medicine and dentistry: orthopedic applications, , Academic Press, San Diego, CA p. 335-46; 
Bucholz, R., Nonallograft osteoconductive bone grafts substitutes (2002) Clin Orthop, 395, pp. 44-52; 
Kirker-Head, C.A., Potential applications and delivery strategies of bone morphogenetic proteins (2000) Adv Drug Deliv Rev, 43, pp. 65-92; 
Chen, Y., Luk, K.D.K., Cheung, K.M.C., Xu, R., Lin, M.C., Lu, W.W., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors (2003) Gene Ther, 10 (16), pp. 1345-1353; 
Tseng, W.-C., Huang, L., Liposome-based gene therapy (1998) Res Focus, 1 (5); 
Leach, J.K., Mooney, D., Bone engineering by controlled delivery of osteoinductive molecules and cells (2004) Expert Opin, 4 (7); 
Dragoo, J.L., Choi, J.Y., Lieberman, J.R., Huang, J., Zuk, P.A., Zhang, J., Bone induction by BMP-2 transduced stem cells derived from human fat (2003) J Orthop Res, 21 (4), pp. 622-629; 
Eliyahu, H., Barenholz, Y., Domb, A.J., Polymers for DNA delivery (2005) Molecules, 10, pp. 34-64; 
Penque, D., Terapia g{\'e}nica: um objectivo ou uma realidade (2000) Boletim Biotecnol, 66, pp. 16-19; 
Winn, S.R., Hu, Y., Sfeir, C., Hollinger, J.O., Gene therapy approaches for modulating bone regeneration (2000) Adv Drug Deliv Rev, 42 (1-2), pp. 121-138; 
Lima, N., Mota, M., (2003) Biotecnologia, fundamentos e aplica{\c c}{\~o}es, , Lidel, Lisboa p. 368-73; 
Luo, D., Saltzman, W.M., Synthetic DNA delivery systems (2000) Nat Biotechnol, 18, pp. 33-37; 
Kikuchi, H., Suzuki, N., Ebihara, K., Morita, H., Ishii, Y., Kikuchi, A., Gene delivery using liposome technology (1999) J Control Release, 62 (1-2), pp. 269-277; 
Liu, F., Huang, L., Development of non-viral vectors for systemic gene delivery (2002) J Control Release, 78 (1-3), pp. 259-266; 
Girao da Cruz, M.T., Simoes, S., Pires, P.P.C., Nir, S., Pedroso de Lima, M.C., Kinetic analysis of the initial steps involved in lipoplex-cell interactions: effect of various factors that influence transfection activity (2001) Biochim Biophys Acta (BBA) - Biomembr, 1510 (1-2), pp. 136-151; 
Zuidam, N.J., Barenholz, Y., Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery (1998) Biochim Biophys Acta (BBA) - Biomembr, 1368 (1), pp. 115-128; 
Tros de Ilarduya, C., Arangoa, M.A., Moreno-Aliaga, M.J., Duzgunes, N., Enhanced gene delivery in vitro and in vivo by improved transferrin-lipoplexes (2002) Biochim Biophys Acta (BBA) - Biomembr, 1561 (2), pp. 209-221; 
Simoes, S., Pires, P., Cruz, M.T.G.d., Duzgunes, N., Pedroso de Lima, M.C., Gene delivery by cationic liposome-DNA complexes containing transferrin or serum albumin (2003) Methods Enzymol, 373, pp. 369-383; 
Woo-Jeong, C., Jin-Ki, K., Sung-Hee, C., Jeong-Sook, P., Woong Shick, A., Kima, C.-K., Low toxicity of cationic lipid-based emulsion for gene transfer (2004) Biomaterials, 25 (27), pp. 5893-5903; 
Koe, G.S., Way, H.L., Quentingco, G.M., Smith, J.G., Wedeking, T., Niven, R.W., The effect of mixing on the formation of DNA/liposome complexes (1997) Pharmacy, p. 14; 
Lasic, D.D., Templeton, N.S., Liposomes in gene therapy (1996) Adv Drug Deliv Rev, 20 (2-3), pp. 221-266; 
Pedroso de Lima, M.C., Simoes, S., Pires, P., Faneca, H., Duzgunes, N., Cationic lipid-DNA complexes in gene delivery: from biophysics to biological applications (2001) Adv Drug Deliv Rev, 47 (2-3), pp. 277-294; 
Hirsch-Lerner, D., Zhang, M., Eliyahu, H., Ferrari, M.E., Wheeler, C.J., Barenholz, Y., Effect of "helper lipid" on lipoplex electrostatics (2005) Biochim Biophys Acta (BBA) - Biomembr, 1714 (2), pp. 71-84; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57 (1), pp. 57-62; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles (2003) Biomaterials, 24 (7), pp. 1255-1264; 
Mislick, K.A., Baldeschwieler, J.D., Evidence for role of proteoglycans in cation-mediated gene transfer (1996) Proc Natl Acad Sci USA, 93 (22), pp. 12349-12354; 
Duzgunes, N., Nir, S., Mechanisms and kinetics of liposome-cell interactions (1999) Adv Drug Deliv Rev, 40 (1-2), pp. 3-18; 
Radwan Almofti, M., Harashima, H., Shinohara, Y., Almofti, A., Baba, Y., Kiwada, H., Cationic liposome-mediated gene delivery: biophysical study and mechanism of internalization (2003) Arch Biochem Biophys, 410 (2), pp. 246-253; 
Sim{\~o}es, S., Filipe, A., Faneca, H., Mano, M., Penacho, N., Duzgunes, N., Cationic liposomes for gene delivery (2005) Expert Opin, 2 (2); 
Rejman, J., Bragonzi, A., Conese, M., Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes (2005) Mol Ther, 12 (3), pp. 468-474
},
	month = {2009///},
	pages = {2142 - 2151},
	abstract = {Modulation of the biological pathways responsible for fracture repair and osteogenisis may accelerate regeneration. Gene therapy is an alternative method for the release of osteogenisis-stimulating proteins into tissues. The development of vectors for gene release is still a problem in terms of ethics and techniques. In this work we evaluated whether cationic liposomes constitute a valuable strategy for the release of genetic material into bone tissue cells as non-viral vectors. Liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium propane (DOTAP)-2-dioleoyl-sn-glycero-3-phosphatidylethanolamine and DOTAP-cholesterol, and characterized according to their size, zeta potential, DNA protection capacity and cytotoxicity. Transfection studies were also carried out using pCMVβ-gal plasmid in two osteoblastic cell lines (MG63 and MC3T3-E1) and in the 294T line, varying the charge ratio and the applied DNA dose. Inclusion of transferrin to increase the expression was also tested. The results suggest that there is great dependency between the transfection activity and the lipid formulation, the charge ratios of the complexes, the applied DNA dose and the cell type. There were even some differences concerning both osteoblastic lines under study. The cells of the MC3T3-E1 line present greater expression levels than the cells of the MG-63 line. The conjugation of the transferrin with the complexes contributes to the increase in transfection levels, possibly due to an increase in internalization of complexes. It is thus a good strategy for inducing the expression of specific genes in osteoblast-like cells. {\textcopyright} 2009 Acta Materialia Inc.},
	keywords = {1,2 dioleoyl 3 trimethylammoniopropane, animal cell, Animals, article, bone cell, bone development, bone tissue, Cationic liposomes, Cell Line, cell type, complex formation, controlled study, Cytotoxicity, DNA, drug mechanism, gene expression regulation, Gene therapy, Gene transfer, genetic transfection, Genetic Vectors, human, human cell, internalization, lipoplex, liposome, Liposomes, materials testing, Mice, nonhuman, nonviral gene delivery system, osteoblast, Osteoblasts, Osteogenesis, Particle Size, plasmid, priority journal, Transfection, transferrin, Zeta potential},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67349188484\&partnerID=40\&md5=b675ab7e6e97523c5d8923fa6e7f0d8b},
	author = {Oliveira, A.C. and Ferraz, M.P. and Monteiro, F.J. and Sim{\~o}es, S.}
}
@article {2308,
	title = {Physical characterization of hydroxyapatite porous scaffolds for tissue engineering},
	journal = {Materials Science and Engineering CMater. Sci. Eng. C},
	volume = {29},
	year = {2009},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.msec.2008.09.052Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: smsilva@ineb.up.ptReferences: Jones, A.C., Milthorpe, B., Averdunk, H., Limaye, A., Senden, T.J., Sakellariou, A., (2004) Biomaterials, 25 (20), p. 4947;Karageorgiou, V., Kaplan, D., (2005) Biomaterials, 26 (27), p. 5474; 
Sepulveda, P., Ortega, F.S., Innocentini, M.D.M., Pandolfelli, V.C., (2000) Journal of the American Ceramic Society, 83 (12), p. 3021; 
Woodard, J.R., Hilldore, A.J., Lan, S.K., Park, C.J., Morgan, A.W., Eurell, J.A., (2007) Biomaterials, 28 (1), p. 45; 
Harris, L.D., Kim, B.S., Mooney, D.J., (1998) Journal of Biomedical Materials Research, 42 (3), p. 396; 
Cyster, L.A., Grant, D.M., Howdle, S.M., Rose, F.R., Irvine, D.J., Freeman, D., (2005) Biomaterials, 26 (7), p. 697; 
Fu, Q., Rahaman, M.N., Bal, B.S., Huang, W., Day, D.E., (2007) Journal of Biomedical Materials Research A, 82 (1), p. 222; 
Chang, B.S., Lee, C.K., Hong, K.S., Youn, H.J., Ryu, H.S., Chung, S.S., (2000) Biomaterials, 21 (12), p. 1291; 
Chu, T.M., Orton, D.G., Hollister, S.J., Feinberg, S.E., Halloran, J.W., (2002) Biomaterials, 23 (5), p. 1283; 
Fu, Q., Rahaman, M.N., Dogan, F., Bal, B.S., (2007) Journal of Biomedical Materials Research B, Applied Biomaterials; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2004) Advanced Materials Forum Ii, 455-456, p. 358; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., (2004) Bioceramics, 254-2, p. 997. , (16); 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., (2008) Journal of Materials Science-Materials in Medicine, 19 (2), p. 855; 
de Groot, K., (1980) Biomaterials, 1 (1), p. 47; 
Heise, U., Osborn, J.F., Duwe, F., (1990) International Orthopaedics, 14 (3), p. 329; 
Kuboki, Y., Takita, H., Kobayashi, D., Tsuruga, E., Inoue, M., Murata, M., (1998) Journal of Biomedical Materials Research, 39 (2), p. 190; 
Ramay, H.R., Zhang, M., (2003) Biomaterials, 24 (19), p. 3293; 
Kim, H.W., Knowles, J.C., Kim, H.E., (2005) Journal of Materials Science, 16 (3), p. 189; 
Iwano, T., Kurosawa, H., Murase, K., Takeuchi, H., Ohkubo, Y., (1991) Clinical Orthopaedics and Related Research, 268, p. 243; 
Tieliewuhan, Y., Hirata, I., Sasaki, A., Minagi, H., Okazaki, M., (2004) Dental Materials Journal, 23 (3), p. 258; 
Yunoki, S., Ikoma, T., Tsuchiya, A., Monkawa, A., Ohta, K., Sotome, S., (2007) Journal of Biomedical Materials Research B, Applied Biomaterials, 80 (1), p. 166; 
Zhang, Q.Q., Ren, L., Wang, C., Liu, L.R., Wen, X.J., Liu, Y.H., (1996) Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 24 (6), p. 693; 
Zhang, S.M., Cui, F.Z., Liao, S.S., Zhu, Y., Han, L., (2003) Journal of Materials Science, 14 (7), p. 641; 
Yunoki, S., Ikoma, T., Monkawa, A., Marukawa, E., Sotome, S., Shinomiya, K., (2007) Journal of Biomaterials Science, 18 (4), p. 393; 
Teixeira, S., Oliveira, S., Ferraz, M.P., Monteiro, F.J., (2008) Key Engineering Materials, 361-363 (II), p. 947; 
Gibson, I.R., Best, S.M., Bonfield, W., (1999) Journal of Biomedical Materials Research, 44 (4), p. 422; 
Kamitakahara, M., Ohtsuki, C., Kozaka, Y., Ogata, S., Tanihara, M., Miyazaki, T., (2006) Journal of the Ceramic Society of Japan, 114 (1325), p. 82; 
Kim, H.W., Deng, Y., Miranda, P., Pajares, A., Kim, D.K., Kim, H.E., (2001) Effect of Flaw State on the Strength of Brittle Coatings on Soft Substrates, p. 2377
},
	month = {2009///},
	pages = {1510 - 1514},
	abstract = {The present study refers to the preparation and characterization of porous hydroxyapatite scaffolds to be used as matrices for bone regeneration or as specific release vehicles. Ceramics are widely used for bone tissue engineering purposes and in this study, hydroxyapatite porous scaffolds were produced using the polymer replication method. Polyurethane sponges were used as templates and impregnated with a ceramic slurry at different ratios, and sintered at 1300~{\textdegree}C following a specific thermal cycle. The characteristics of the hydroxyapatite porous scaffolds and respective powder used as starting material, were investigated by using scanning electron microscopy, particle size distribution, X-ray diffraction, Fourier transformed infrared spectroscopy and compressive mechanical testing techniques. It was possible to produce highly porous hydroxyapatite scaffolds presenting micro and macropores and pore interconnectivity. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Apatite, Bone, Bone regenerations, Bone tissue engineerings, Ceramic materials, Ceramic slurries, Fourier-transformed infrared spectroscopies, hydroxyapatite, infrared spectroscopy, Macro-pores, Mechanical testing, Physical characterizations, Polymer blends, Polymer replications, Polymers, Polyurethane sponges, Pore interconnectivity, Porous hydroxyapatites, Porous scaffolds, Scaffolds, Scanning electron microscopy, Spectroscopic analysis, Starting materials, Thermal cycles, Tissue engineering, X- ray diffractions},
	isbn = {09284931 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67349181295\&partnerID=40\&md5=ea36fd01a8565ecbd7230948b317995d},
	author = {Teixeira, S. and Rodriguez, M.A. and Pena, P. and De Aza, A.H. and De Aza, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2358,
	title = {Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {19},
	year = {2008},
	note = {Cited By (since 1996): 23Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-007-3005-xPubMed ID: 17665126Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: smsilva@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Ceramics; Durapatite, 1306-06-5References: Jones, A.C., (2004) Biomaterials, 25, p. 4947;Karaggeorgiou, V., (2005) Biomaterials, 27, p. 5474; 
Leon, C.A., Leon, Y., (1998) Adv. Colloid. Interface Sci., 76-77, p. 341; 
Kuboki, Y., Takita, H., Kobayashi, D., Tsuruga, E., Inoue, M., Murata, M., (1998) J. Biomed. Mater. Res., 39, p. 190. , 2; 
Heise, U., Osborn, J.F., Duwe, F., (1990) Int. Orthop., 14, p. 329; 
Bucholz, R.W., Carlton, A., Holmes, R.E., (1987) Orthop. Clin. North. Am., 18, p. 323; 
Hill, P.A., (1998) Br. J. Orthod., 25, p. 101; 
Martin, R.B., (1999) Mater. Sci. Forum, 293, p. 5; 
Nunes, C.R., Simske, S.J., Sachdeva, R., Wolford, L.M., (1997) J. Biomed. Mater. Res., 36, p. 560; 
Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Key Eng. Mater., 254-256, p. 997; 
Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Mater. Sci. Forum, 455-456, p. 358; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Porous, H.A., (2005) Key Eng. Mater., 284-286; 
Teixeira, S.M., Queiroz, A.C., Monteiro, F.J., Ferraz, M.P., In vitro analysis of macroporous ceramic matrices: Preliminary results (2005) 19th European Conference on Biomaterials, , PP-P173; Naples, Italy, Sept 11-15
},
	month = {2008///},
	pages = {855 - 859},
	abstract = {Hydroxyapatite porous scaffolds can be used for tissue engineering applications since they can serve as templates for cell adhesion, proliferation and ultimately for tissue repair. One way to address this issue is to evaluate the cell adhesion using several characterization techniques namely, cytotoxicity assays and cell visualization. On the other hand, when using highly macroporous scaffolds some techniques may not be adequate for evaluation, such as MTT. In this work, cytotoxicity assay (MTS), scanning electron microscopy (SEM) and Confocal laser scanning microscopy (CLSM) were used to evaluate cell adhesion in highly macroporous hydroxyapatite scaffolds. It was possible to observe that some techniques were not suitable to evaluate cell adhesion. In addition, it was shown that for this kind of scaffolds, confocal laser scanning microscopy is a powerful tool for cell adhesion and proliferation evaluation. {\textcopyright} 2007 Springer Science+Business Media, LLC.},
	keywords = {article, Bioassay, Biocompatibility, Biocompatible Materials, cell adhesion, Cell Line, Tumor, cell proliferation, cell structure, Ceramic materials, ceramics, confocal laser microscopy, controlled study, Cytotoxicity, Cytotoxicity assay, Durapatite, human, human cell, Humans, hydroxyapatite, Macroporous ceramic scaffolds, Microscopy, Confocal, Microscopy, Electron, Scanning, Osteoblasts, priority journal, Scanning electron microscopy, Tissue engineering, Tissue Scaffolds},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-39049162092\&partnerID=40\&md5=500dfec2998ea8943e9a38309d2b7e5b},
	author = {Teixeira, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2342,
	title = {Comparative study of nanohydroxyapatite microspheres for medical applications},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {86},
	year = {2008},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31634PubMed ID: 17975824Language of Original Document: EnglishCorrespondence Address: Mateus, A. Y. P.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium hydroxide, 1305-62-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium, 7440-70-2; dodecyl sulfate sodium, 151-21-3; neutral red, 553-24-2; phosphoric acid, 7664-38-2; Biocompatible Materials; Durapatite, 1306-06-5; PowdersReferences: Jarcho, M., Calcium phosphates ceramics as hard tissue prosthetics (1981) Clin Orthop Relat Res, 157, pp. 259-278;Bucholz, R., Carlton, A., Holmes, R., Hydroxyapatite and tricalcium phosphate bone graft substitutes (1987) Orthop Clin N Am, 18, pp. 323-334; 
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},
	month = {2008///},
	pages = {483 - 493},
	abstract = {This study concerns the preparation, physical, and in vitro characterization of two different types of hydroxyapatite (HA) microspheres, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using the chemical precipitation synthesis with H                        3PO
                        4, Ca(OH) 
                        2, and a surfactant, SDS (sodium dodecyl sulfate), as starting reagents. The HA powders were dispersed in a sodium alginate solution, and spherical particles were obtained by droplet extrusion coupled with ionotropic gelation in the presence of Ca
                        2+. These were subsequently sintered to produce HA-1 and HA-2 microspheres with a uniform size and interconnected microporosity. Both powders and microspheres were characterized using FTIR and X-ray diffraction. Moreover, SEM and mercury intrusion porosimetry were used to analyze the microspheres, and TEM was used to analyze the powders. Results showed that pure HA and mixtures of HA/β-TCP in the nanometer range and needlelike shape were obtained for HA-1 and HA-2 powders, respectively. Neutral Red, scanning electron microscopy and confocal microscopy were used to evaluate the behavior of osteoblastic-like MG-63 cells cultured on HA microspheres surfaces for 7 days. Results showed that good adhesion and proliferation of osteoblasts on the HA microspheres surface. Cells built bridges between adjacent microspheres, forming microspheres-cells clusters in both types of materials. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {alginic acid, analytic method, Apatite, article, Biocompatible Materials, Biological materials, biomaterial, Bone Regeneration, Calcium, calcium hydroxide, calcium phosphate, cell adhesion, Cell Line, cell proliferation, Chemical precipitation, Chemotherapy, Comparative studies, comparative study, controlled study, dodecyl sulfate sodium, Drug delivery, Drug delivery system (DDS), Drug Delivery Systems, Drug dosage, Durapatite, Gelation, human, human cell, Humans, hydroxyapatite, Hydroxyapatite (HAP), Hydroxylation, In vitro characterization, in vitro study, infrared spectroscopy, materials testing, Medical applications, Mercury intrusion porosimetry, microsphere, Microspheres, Nano-hydroxyapatite (n-HA), Nanohydroxyapatite, nanoparticle, nanoparticles, neutral red, osteoblast, Osteoblasts, Particle Size, phosphoric acid, porosity, powder, Powders, precipitation, Precipitation (chemical), reagent, Regeneration (renovating), Scanning electron microscopy, surfactant, synthesis, Synthesis (chemical), X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47049103112\&partnerID=40\&md5=138b3e2f3a97d8b398546d3f85567a0f},
	author = {Mateus, A.Y.P. and Barrias, C.C. and Ribeiro, C. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2354,
	title = {PLD bioactive ceramic films: The influence of CaO-P                    2O 
                    5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells
},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {19},
	year = {2008},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-007-3331-zPubMed ID: 18058197Language of Original Document: EnglishCorrespondence Address: Oliveira, G. M.; Universidade Fernando Pessoa, Pra{\c c}a Nove de Abril 349, 4249-004 Porto, Portugal; email: gisela@ufp.ptChemicals/CAS: calcium oxide, 1305-78-8; hydroxyapatite, 1306-06-5, 51198-94-8; oxide, 16833-27-5; Biocompatible Materials; Calcium Compounds; Ceramics; Durapatite, 1306-06-5; Oxides; Phosphorus Compounds; Polystyrenes; lime, 1305-78-8; phosphorus pentoxide, 1314-56-3References: Hench, L.L., (1991) J. Am. Ceram. Soc., 74, p. 1487;Hench, L.L., (1998) Biomaterials, 19, p. 1419; 
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},
	month = {2008///},
	pages = {1775 - 1785},
	abstract = {This work consists on the evaluation of the in vitro performance of Ti6Al4V samples PLD (pulsed laser deposition) coated with hydroxyapatite, both pure and mixed with a CaO-P                        2O
                        5 glass. Previous studies on immersion of PLD coatings in SBF, showed that the immersion apatite films did not present the usual cauliflower morphology but replicated the original columnar structure and exhibited good bioactivity. However, the influence of glass associated to hydroxyapatite concerning adhesion, proliferation and morphology of MG63 cells on the films surface was unclear. In this study, the performance of these PLD coated samples was evaluated, not only following the physical-chemical transformations resulting from the SBF immersion, but also evaluating the cytocompatibility in contact with osteoblast-like MG63 cells. SEM and AFM confirmed that the bioactive ceramic PLD films reproduce the substrate{\textquoteright}s surface topography and that the films presented good adherence and uniform surface roughness. Physical-chemical phenomena occurring during immersion in SBF did not modify the original columnar structure. In contact with MG63 cells, coated samples exhibited very good acceptance and cytocompatibility when compared to control. The glass mixed with hydroxyapatite induced higher cellular proliferation. Cells grown on these samples presented many filipodia and granular structures, typical features of osteoblasts. {\textcopyright} 2007 Springer Science+Business Media, LLC.
},
	keywords = {Apatite, Apatite films, Bioactive ceramic films, Bioactive glass, Bioactivity, Bioceramics, Biocompatible Materials, Biofilms, Brassica oleracea var. botrytis, Calcium Compounds, calcium oxide, Cauliflower morphology, cell adhesion, cell proliferation, cell structure, cell survival, ceramics, Columnar structure, conference paper, controlled study, Durapatite, glass, human, human cell, Humans, hydroxyapatite, Lasers, materials testing, Microscopy, Atomic Force, Microscopy, Electron, Scanning, morphology, osteoblast, Osteoblasts, oxide, Oxides, phosphorous pentoxide, Phosphorus Compounds, Polystyrenes, priority journal, Pulsed laser deposition, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-40949140642\&partnerID=40\&md5=de09d5133a0616668cb5e57135d91ae6},
	author = {Oliveira, G.M. and Ferraz, M.P. and Gonz{\'a}lez, P.G. and Serra, J. and Leon, B. and P{\`e}rez-Amor, M. and Monteiro, F.J.}
}
@book {2363,
	title = {Three dimensional macroporous calcium phosphate scaffolds for bone tissue engineering},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {361-363 II},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB, Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Rose, F.R., Cyster, L.A., Grant, D.M., Scotchford, C.A., Howdle, S.M., Shakesheff, K.M.K.M., In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel (2004) Biomaterials, 25 (24), pp. 5507-5514;Anthony, C., Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging (2004) Biomaterials, 25 (20), pp. 4947-4954; 
Teixeira, S., Fernandes, H.M., de Boer, J., Ferraz, M.P., Monteiro, F.J., Mesenchymal stem cell proliferation and morphological analysis on interconnected macroporous structures (2007) Key Engineering Materials, 330-332, pp. 1129-1132. , Vols
},
	month = {2008///},
	pages = {947 - 950},
	abstract = {Calcium phosphate ceramics are widely used as bone substitutes since they are biocompatible and bioactive. Having a chemical composition close to natural bone, calcium phosphate ceramics are promising bone substitute materials in orthopaedics, maxillofacial surgery and dentistry. Hydroxyapatite (HA) and tricalcium phosphate (TCP) are the most commonly used calcium phosphates, because their calcium/phosphorus (Ca/P) ratios are close to that of natural bone and they are relatively stable in physiological environment. HA is a major constituent of bone materials and is resorbed after a long time of residence in the body. In this work, highly porous hydroxyapatite scaffolds were produced by polymer replication method and their properties evaluated by Scanning Electron Microscopy (SEM) and micro computerized tomography (μ-CT).},
	keywords = {Biocompatibility, Bone, calcium phosphate, Calcium phosphates, Computerized tomography, Maxillofacial prostheses, Maxillofacial surgery, Micro computerized tomography, Porous, Scaffolds, Scanning electron microscopy, Tissue engineering},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38149045804\&partnerID=40\&md5=d137e6232587d31ba95a0c04c781c90a},
	author = {Teixeira, S. and Oliveira, S. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2378,
	title = {Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {81},
	year = {2007},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31073PubMed ID: 17252543Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Ceramics; Durapatite, 1306-06-5Manufacturers: Plasma BiotalReferences: Teixeira S, Queiroz AC, Monteiro FJ, Ferraz MP, Vilar R, Eugenio S. Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results. Bioceramics 2006;18 (Parts 1, 2):309-311, 105-108Fernandez-Pradas, J.M., Cleries, L., Martinez, E., Sardin, G., Esteve, J., Morenza, J.L., Influence of thickness on the properties of hydroxyapatite coatings deposited by KrF laser ablation (2001) Biomaterials, 22, pp. 2171-2175;Fernandez-Pradas, J.M., Cleries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the deposition rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys A: Mater Sci Process, 72, pp. 613-618; 
Foltyn, S.R., Dye, R., Ott, K.C., Peterson, E., Hubbard, K.M., Hutchinson, W., Muenchausen, R.E., Wu, X.D., Target modification in the excimer laser deposition of YBa 2Cu 3O 7-x thin films (1991) Appl Phys Lett, 59, pp. 594-596; 
Khor, K.A., Vreeling, A., Dong, Z.L., Cheang, P., Laser treatment of plasma sprayed HA coatings (1999) Mater Sci Eng A: Struct Mater Prop Microstruct Process, 266 (1-2), pp. 1-7; 
Ricci, J.L., Alexander, H., Laser microtexturing of implant surfaces for enhanced tissue integration (2001) Key Engineering Mater, pp. 198-199,179-202; 
Galli, C., Guizzardi, S., Passeri, G., Martini, D., Tinti, A., Mauro, G., Macaluso, G.M., Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces (2005) J Periodontol, 76, pp. 364-372; 
Oliveira, V., Conde, O., Vilar, R., UV laser micromachining of ceramic materials: Formation of columnar topographies (2001) Adv Eng Mater, 3 (1-2), pp. 75-81; 
Serra, P., FernandezPradas, J., Sardin, G., Morenza, J.L., Interaction effects of an excimer laser beam with hydroxyapatite targets (1997) Appl Surf Sci, 110, pp. 384-388; 
Serra, P., Morenza, J.L., Fluence dependence of hydroxyapatite laser ablation plumes (1998) Thin Solid Films, 335 (1-2), pp. 43-48; 
Duncan, A.C., Weisbuch, F., Rouais, F., Lazare, S., Baquey, C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosens Bioelectron, 17, pp. 413-426; 
Walboomers, X.F., Croes, H.J.E., Ginsel, L.A., Jansen, J.A., Contact guidance of rat fibroblasts on various implant materials (1999) J Biomed Mater Res, 47, pp. 204-212; 
Carvalho, P., Manzanares, M.C., Franch, J., Belmonte, A.M., Lafuente, M.P., Serra, I., Fernandez-Pradas, J.M., Morenza, J.L., Osteointegration of calcium phosphate coatings obtained by pulsed laser deposition (2003) J Dent Res, 82, p. 573; 
Ahmad, M., McCarthy, M., Gronowicz, G., An in vitro model for mineralization of human osteoblast-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220; 
Matsuura, T., Hosokawa, R., Okamoto, K., Kimoto, T., Akagawa, Y., Diverse mechanisms of osteoblast spreading on hydroxyapatite and titanium (2000) Biomaterials, 21, pp. 1121-1127; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Giardino, R., Comparative interspecies investigation on osteoblast cultures: Data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57, pp. 57-62; 
Boyan, B.D., Lossdoerfer, S., Wang, L., Zhao, G., Lohmann, C.H., Cochran, D.L., Schwartz, Z., Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies (2003) Eur Cell Mater, 6, pp. 22-27; 
Anselme, K., Bigerelle, M., Topography effects of pure titanium substrates on human osteoblast long-term adhesion (2005) Acta Biomater, 1, pp. 211-222; 
Anselme, K., Linez, P., Bigerelle, M., Le Maguer, D., Le Maguer, A., Hardouin, P., Hildebrand, H.F., Leroy, J.M., The relative influence of the topography and chemistry of TiAl 6V 4 surfaces on osteoblastic cell behaviour (2000) Biomaterials, 21, pp. 1567-1577; 
Anselme, K., Noel, B., Hardouin, P., Human osteoblast adhesion on titanium alloy, stainless steel, glass and plastic substrates with same surface topography (1999) J Mater Sci: Mater Med, 10, pp. 815-819; 
Cleries L, Fernandez-Pradas JM, Morenza JL. Bone growth on and resorption of calcium phosphate coatings obtained by pulsed laser deposition. J Biomed Mater Res 2000;49:43. Erratum 2001;57:473Deligianni, D.D., Katsala, N., Ladas, S., Sotiropoulou, D., Amedee, J., Missirlis, Y.F., Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption (2001) Biomaterials, 22, pp. 1241-1251; 
dos Santos, E.A., Farina, M., Soares, G.A., Specific proliferation rates of human osteoblasts on calcium phosphate surfaces with variable concentrations of α-TCP (2007) Mater Sci Eng, 27, pp. 61-66; 
Hao, L., Lawrence, J., Laser surface treatment of magnesia partially stabilized zirconia for enhanced human skin fibroblast cell response (2004) J Laser Appl, 16, pp. 55-64; 
Kieswetter, K., Schwartz, Z., Dean, D.D., Boyan, B.D., The role of implant surface characteristics in the healing of bone (1996) Crit Rev Oral Biol Med, 7, pp. 329-345; 
Salda{\~n}a, L., Gonz{\'n}lez-Carrasco, J.L., Rodriguez, M., Munuera, L., Vilaboa, N., Osteoblast response to plasma-spray porous Ti 6AL 4V coating on substrates of identical alloy (2006) J Biomed Mater Res A, 77, pp. 608-617; 
Lincks, J., Boyan, B.D., Blanchard, C.R., Lohmann, C.H., Liu, Y., Cochran, D.L., Dean, D.D., Schwartz, Z., Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition (1998) Biomaterials, 19, pp. 2219-2232; 
Martin, J.Y., Dean, D.D., Cochran, D.L., Simpson, J., Boyan, B.D., Schwartz, Z., Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces (1996) Clin Oral Implants Res, 7, pp. 27-37; 
Noth, U., Hendrich, C., Merklein, F., Altvater, T., Rader, C.P., Schutze, N., Eulert, J., Thull, R., Standardised testing of bone-implant surfaces using an osteoblast cell culture system. II. Titanium surfaces of varying degrees of roughness (1999) Biomedizinische Technik, 44 (1-2), pp. 6-11; 
Ponsonnet, L., Reybier, K., Jaffrezic, N., Comte, V., Lagneau, C., Lissac, M., Martelet, C., Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour (2003) Mater Sci Eng C: Biomimetic Supramolecular Syst, 23, pp. 551-560; 
Ramires, P.A., Romito, A., Cosentino, F., Milella, E., The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour (2001) Biomaterials, 22, pp. 1467-1474; 
Redey, S.A., Nardin, M., Bernache-Assolant, D., Rey, C., Delannoy, P., Sedel, L., Marie, P.J., Behavior of human osteoblastic cells on stoichiometric hydroxyapatite and type A carbonate apatite: Role of surface energy (2000) J Biomed Mater Res, 50, pp. 353-364; 
Rosa, A.L., Beloti, M.M., van Noort, R., Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography (2003) Dent Mater, 19, pp. 768-772; 
Schmidt, C., Ignatius, A.A., Claes, L.E., Proliferation and differentiation parameters of human osteoblasts on titanium and steel surfaces (2001) J Biomed Mater Res, 54, pp. 209-215; 
Schwartz, Z., Kieswetter, K., Dean, D.D., Boyan, B.D., Underlying mechanisms at the bone-surface interface during regeneration (1997) J Periodontal Res, 32, pp. 166-171; 
Schwartz, Z., Martin, J.Y., Dean, D.D., Simpson, J., Cochran, D.L., Boyan, B.D., Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation (1996) J Biomed Mater Res, 30, pp. 145-155; 
Wang, J., de Boer, J., de Groot, K., Preparation and characterization of electrodeposited calcium phosphate/chitosan coating on Ti 6Al 4V plates (2004) J Dent Res, 83, pp. 296-301; 
Xavier, S.P., Carvalho, P.S.P., Beloti, M.M., Rosa, A.L., Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments (2003) J Dent, 31, pp. 173-180
},
	month = {2007///},
	pages = {920 - 929},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture, and microchemistry. The surface topography eventually affects the nature and the intensity of the interactions that occur at biomaterial- biological interface (cell adhesion, mobility, spreading, and proliferation). Therefore, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (in this case, osteoblasts). There are a number of substrates that already have been studied in terms of surface topography; however, few studies are related to hydroxyapatite (HA) substrates. As it is well established, HA is a well-known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense HA substrates was altered by using KFr excimer laser. The surface was characterized by atomic force microscopy and contact angle measurements, while the cell distribution and morphology was assessed by scanning electron microscopy and confocal laser scanning microscopy. Results revealed that the surface is characterized by a homogeneous columnar structure with high specific area. Moreover, cells were able to attach and spread on the surface of the samples, and gradually grow into nearly confluent monolayers. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {article, Atomic force microscopy, Biocompatibility, biomaterial, cell adhesion, Cell Communication, Cell Line, Tumor, cell motility, cell proliferation, cell spreading, cellular distribution, ceramic prosthesis, ceramics, chemistry, confocal laser microscopy, controlled study, Durapatite, Excimer laser, Excimer lasers, human, human cell, Humans, hydroxyapatite, laser, Lasers, material coating, Microchemistry, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Electron, Scanning, Microtexture, monolayer culture, osteoblast, Osteoblasts, reproducibility, Scanning electron microscopy, Surface microtopography, Surface modification, Surface Properties, surface property, Surface topography, Surface treatment, Tissue engineering},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249742544\&partnerID=40\&md5=b6cb0aaa7b8e4a267b97d91d7171862f},
	author = {Teixeira, S. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@book {2383,
	title = {Mesenchymal stem cell proliferation and morphological analysis on interconnected macroporous structures},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 II},
	year = {2007},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre,823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Mastrogiacomo, M., (2006) Biomaterials, 27, p. 3230;Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Materials, pp. 455,358. , Science Forum; 
Queiroz, A.C., Teixeira, S., Monteiro, F.J., Santos, J.D., (2004) Key Engineering Materials, pp. 254,997; 
Moroni, L., (2006) Biomaterials, 27, p. 4911
},
	month = {2007///},
	pages = {1129 - 1132},
	abstract = {Calcium phosphate ceramics are widely used as bone substitutes since they are biocompatible and bioactive. Given that their chemical composition is close to natural bone, calcium phosphate ceramics are promising bone substitute materials in orthopaedics, maxillofacial surgery and dentistry. Hydroxyapatite (HA) and tricalcium phosphate (TCP) are the most commonly used calcium phosphates, because their calcium/phosphorus (Ca/P) ratios are close to that of natural bone and they are relatively stable in physiological environment. Furthermore, other critical parameters must be accomplished when designing a biomaterial for bone regeneration, namely: pore size, shape and interconnectivity [1]. Porosity is one of the most important factors since it influences the adhesion, migration nutrient supply and ultimately, proliferation of mesenchymal stem cells. In this study, HA scaffolds with controlled porosity were obtained and their capacity to support human and rat mesenchymal stem cells attachment and proliferation was evaluated.},
	keywords = {Biomaterials, Calcium Compounds, cell adhesion, cytology, hydroxyapatite, Macroporous ceramics, Mesenchymal Stem Cells, orthopedics, Phase composition, Physiological models, porosity, Tricalcium phosphate (TCP)},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846317063\&partnerID=40\&md5=ba9f628d2ad403a6ab70a617a1c9b273},
	author = {Teixeira, S. and Fernandes, H.M. and De Boer, J. and Ferraz, M.P. and Monteiro, F.J.}
}
@book {2384,
	title = {Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 I},
	year = {2007},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Pataquiva Mateus, A.Y.; INEB - Institute de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptReferences: Liou, S., Chen, S., Liu, D., (2003) Biomaterials, 24, p. 3981;Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., (1999) Journal of Biomedical Materials Research, 47, p. 28; 
Grimaldi, G., Weiss, P., Millot, F., Daculsi, G., (1998) Journal of Biomedical Materials Research, 39, p. 660; 
Termenoff, J., Mikos, A., (2000) Biomaterials, 21, p. 2405; 
Best, S., Bonfield, W., Doyle, C., (1989) Bioceramics, p. 68. , Oonishi H, Aoki H, Sawai K, editors, Tokyo: Ishiyaku Euro-America; 
Pataquiva-Mateus, A.Y., Ferraz, M.P., Monteiro, F.J., Journal of Biomedical Materials Research, , Submitted paper
},
	month = {2007///},
	pages = {243 - 246},
	abstract = {This study concerns the preparation and characterisation of microspheres associating alginate and two different types of hydroxyapatite (HA), which are intended to be used as drug delivery systems and bone regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using a chemical precipitation synthesis based on H3PO4, Ca(OH)2 and a surfactant, SDS (sodium dodecylsulphate), as starting reagents. These two powders of nanoHA and alginate were used to prepare two different types of microspheres. Both powders and microspheres were characterised using FTIR, TEM, SEM, mercury porosimetry analysis and X-ray diffraction Results show that pure hydroxyapatite (HA) and mixtures of HA/β-TCP in the nanometre range were obtained from both HA syntheses. Microspheres with different characteristics were obtained from these two types of hydroxyapatite.},
	keywords = {Alginate, Bone, Bone Regeneration, Chemical precipitation, Controlled drug delivery, Fourier transform infrared spectroscopy, hydroxyapatite, Microspheres, Microstructure, nanoparticles, Nanostructured materials, Scanning electron microscopy, Surface active agents, transmission electron microscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846333853\&partnerID=40\&md5=f694c3d331dea2c82aba11cef4dddd59},
	author = {Pataquiva Mateus, A.Y. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2377,
	title = {Nanohydroxyapatite microspheres as delivery system for antibiotics: Release kinetics, antimicrobial activity, and interaction with osteoblasts},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {81},
	year = {2007},
	note = {Cited By (since 1996): 32Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31151PubMed ID: 17252559Language of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mpferraz@ufp.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; amoxicillin plus clavulanic acid, 74469-00-4, 79198-29-1; clindamycin, 18323-44-9; erythromycin, 114-07-8, 70536-18-4; hydroxyapatite, 1306-06-5, 51198-94-8; metronidazole, 39322-38-8, 443-48-1; minocycline, 10118-90-8, 11006-27-2, 13614-98-7; tetracycline, 23843-90-5, 60-54-8, 64-75-5; Anti-Bacterial Agents; Durapatite, 1306-06-5References: Krejci, C.B., Bissada, N.F., Farah, C., Greenwell, H., Clinical-evaluation of porous and nonporous hydroxyapatite in the treatment of human periodontal bony defects (1987) J Periodontol, 58, pp. 521-528;Kinane, D.F., Lindhe, J., Pathogenesis of periodontitis (2002) Clinical Periodontology and Implant Dentistry, pp. 189-225. , Lindhe J, Karring T, Lang NP, editors, Copenhagen: Munksgaard;; 
Slots, J., Armitage, G.C., Cochran, D., Cohen, R.E., Greenstein, G., Mariotti, A., Rethman, M.P., and others, Systemic antibiotics in periodontics (1996) J Periodontol, 67, pp. 831-838; 
Mombelli, A., Antibiotics in periodontal therapy (2002) Clinical Periodontology and Implant Dentistry, , Lindhe J, Karring T, Lang NP, editors, Copenhagen: Munksgaard;; 
Etienne, D., Locally delivered antimicrobials for the treatment of chronic periodontitis (2003) Oral Dis, 9, pp. 45-50; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Porous HA scaffolds for drug releasing (2005) Bioceramics, 17, pp. 407-410; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Porous hydroxyapatite and glass reinforced hydroxyapatite for controlled release of sodium ampicillin (2004) Bioceramics, 16, pp. 997-1000; 
Hannes, P.J., Purvis, J.P., Local anti-infective therapy: Pharmacological agents. A systemic review (2003) Ann Periodontol, 8, pp. 79-98; 
Soskolne, W.A., Heasman, P.A., Stabholz, A., Smart, G.J., Palmer, M., Flashner, M., Newman, H.N., Sustained local delivery of chlorhexidine in the treatment of periodontitis: A multi-center study (1997) J Periodontol, 68, pp. 32-38; 
Greenstein, G., Polson, A., The role of local drug delivery in the management of periodontal diseases: A comprehensive review (1998) J Periodontol, 69, pp. 507-520; 
Killoy, W.J., Chemical treatment of periodontitis: Local delivery of antimicrobials (1998) Int Dent J, 48, pp. 305-315; 
Schwach-Abdellaoui, K., Vivien-Castioni, N., Gurny, R., Local delivery of antimicrobial agents for the treatment of periodontal diseases (2000) Eur J Pharm Biopharm, 50, pp. 83-99; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Development of a system to adsorb drugs onto calcium phosphate materials (2005) J Mater Sci-Mater Med, 16, pp. 641-646; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., Adsorption isotherm of sodium ampicillin onto dense and porous hydroxyapatite (2005) Bioceramics, 17, pp. 387-390; 
Queiroz, A.C., Santos, J.D., Monteiro, F.J., da Silva, MHP dissolution studies of hydroxyapatite and glass-reinforced hydroxyapatite ceramics (2003) Mater Char, 50, pp. 197-202; 
Rauschmann, M.A., Wichelhaus, T.A., Stirnal, V., Dingeldein, E., Zichner, L., Schnettler, R., Alt, V., Nanocrystalline hydroxyapatite and calcium sulphate as biodegradable composite carrier material for local delivery of antibiotics in bone infections (2005) Biomaterials, 26, pp. 2677-2684; 
Queiroz, A.C., Teixeira, S., Santos, J.D., Monteiro, F.J., Production of porus hydroxyapatite with potential for controlled drug delivery (2004) Mater Sci Forum, 455-456, pp. 358-360; 
Daculsi, G., Passuti, N., Effect of the macroporosity for osseous substitution of calcium-phosphate ceramics (1990) Biomaterials, 11, pp. 86-87; 
Gauthier, O., Bouler, J.M., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics (2000) J Biomed Mater Res, 51, pp. 475-483; 
Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., Enhanced functions of osteoblasts on nanophase ceramics (2000) Biomaterials, 21, pp. 1803-1810; 
Webster, T.J., Siegel, R.W., Bizios, R., Enhanced surface and mechanical properties of nanophase ceramics to achieve orthopaedic/dental implant efficacy (2000) Bioceramics, 13, pp. 321-324; 
Liou, S.C., Chen, S.Y., Liu, D.M., Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios (2003) Biomaterials, 24, pp. 3981-3988; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.D., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80; 
Barrias CC, Ribeiro CC, Martins MCL, Barbosa MA, Rodrigues D, Miranda MCS. Calcium phosphate microspheres for localised delivery of a therapeutic enzyme. Bioceramics 2006;18 (Parts 1/2):903-906Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Socransky, S.S., Haffajee, A.D., Microbiology of periodontal disease (2002) Clinical Periodontology and Implant Dentistry, , Lindhe J, editor, Copenhagen: Munksgaard;; 
Slots, J., Selection of antimicrobial agents in periodontal therapy (2002) J Periodontal Res, 37, pp. 389-398; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J Mater Sci-Mater Med, 17, pp. 455-463; 
Barrias, C.C., Ribeiro, C.C., Lamghari, M., S{\'a}, C., Barbosa, M.A., Proliferation, activity and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) J Biomed Mater Res A, 72, pp. 57-66; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Comparative study of injectable material synthesised from alginate and two types of hydroxyapatite Submitted for publicationFerraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P2O5 glass hydroxyapatite double layer plasma sprayed coatings: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Nakamura, T., Kitsogi, T., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J Biomed Mater Res, 24, pp. 471-488; 
Gautier, H., Daculsi, G., Merle, C., Association of vancomycin and calcium phosphate by dynamic compaction: In vitro characterization and microbiological activity (2001) Biomaterials, 22, pp. 2481-2487; 
Kimakhe, S., Bohic, S., Larrose, C., Reynaud, A., Pilet, P., Giumelli, B., Heymann, D., Daculsi, G., Biological activities of sustained polymyxin B release from calcium phosphate biomaterial prepared by dynamic compaction: An in vitro study (1999) J Biomed Mater Res, 47, pp. 18-27; 
Korkusuz, F., Uchida, A., Shinto, Y., Araki, N., Inoue, K., Ono, K., Experimental implant-related osteomyelitis treated by antibiotic-calcium hydroxyapatite ceramic composites (1993) J Bone Joint Surg-Br, 75, pp. 111-114; 
Otsuka, M., Matsuda, Y., Kokubo, T., Yoshihara, S., Nakamura, T., Yamamuro, T., A novel skeletal drug-delivery system using self-setting bioactive glass bone-cement. II. Drug-release from a novel self-setting bioactive glass bone-cement containing cephalexin and its physicochemical properties (1995) J Biomed Mater Res, 29, pp. 33-38; 
Shinto, Y., Uchida, A., Korkusuz, F., Araki, N., Ono, K., Calcium hydroxyapatite ceramic used as a delivery system for antibiotics (1992) J Bone Joint Surg Br, 74, pp. 600-604; 
Yamamura, K., Iwata, H., Yotsuyanagi, T., Synthesis of antibiotic-loaded hydroxyapatite beads and invitro drug release testing (1992) J Biomed Mater Res, 26, pp. 1053-1064; 
Clover, J., Gowen, M., Are Mg-63 and Hos Te85 human osteosarcoma cell-lines representative models of the osteoblastic phenotype? (1994) Bone, 15, pp. 585-591
},
	month = {2007///},
	pages = {994 - 1004},
	abstract = {Severe periodontitis treatment, where massive alveolar bone loss occurs, involves bone defect filling and intensive systemic log-term antibiotics administration. This study aims at developing novel injectable drug delivery systems (nanohydroxyapatite microspheres) with the drug releasing capability for periodontitis treatment and simultaneously initiating the osteointegration process. Materials were characterized by XRD, SEM, inverted stand optical microscope analysis, and mercury porosimetry method. Amoxicillin, amoxicillin + clavulanic acid, and erythromycin were the antibiotics used. Release properties during 28 days from the hydroxyapatite (HA) granules, and two types of nanoHA microspheres were investigated. Biocompatibility was assessed by cytotoxicity assays. HA granules were inadequate, releasing all antibiotic during the first hours. The concentration of antibiotics released in the first days from HA-2 was higher than from HA-1 microspheres, because of the increased porosity and surface area. The release profiles (fast initial release followed by long-term sustained release) of effective doses of antibiotics make these systems good alternatives for antibiotics delivery. Osteoblasts proliferated well on both types of microspheres, being cell growth enhanced in the presence of antibiotics. Erythromycin presented the most beneficial effect. Combining the sustained antibiotic release with the osteoconduction, resorbability, and potential use as injectable bone filling material of porous HA microspheres, these systems provided a forth fold beneficial effect. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {alveolar bone, amoxicillin, amoxicillin plus clavulanic acid, Anti-Bacterial Agents, Antibacterial, antibiotic agent, antibiotic resistance, Antibiotics administration, antimicrobial activity, article, Bacteria, Cell Communication, Cell Death, cell growth, cell kinetics, Cell Line, cell proliferation, clindamycin, concentration response, controlled study, Cytotoxicity, Drug delivery, drug delivery system, Drug Delivery Systems, Drug release, Durapatite, erythromycin, Escherichia coli, human, human cell, Humans, hydroxyapatite, Kinetics, materials testing, metronidazole, Microbial Sensitivity Tests, microsphere, Microspheres, minocycline, Nanohydroxyapatite, nanoparticle, nanoparticles, nonhuman, Optical microscope analysis, osteoblast, Osteoblasts, osteolysis, periodontitis, porosity, Staphylococcus aureus, surface property, sustained drug release, Tetracycline, unclassified drug, X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249689569\&partnerID=40\&md5=5474dac8549e9cd037a96eba13ac9605},
	author = {Ferraz, M.P. and Mateus, A.Y. and Sousa, J.C. and Monteiro, F.J.}
}
@book {2413,
	title = {Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {309-311 I},
	year = {2006},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterlais, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptReferences: Duncan, A.C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosensors and Bioelectronics, 17, pp. 413-426;Walboomers, X.F., Contact guidance of rat fibroblasts on various implant materials (1999) Journal of Biomedical Materials Research, 47, pp. 204-212; 
Queiroz, A.C., Laser surface modifications of hydroxyapatite and glass-reinforced hydroxyapatite (2004) Biomaterials, 25, pp. 4607-4614; 
Ahmad, M., McCarthy, M.B., Gronowicz, G., An in vitro model for mineralization of human osteoblasts-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220
},
	month = {2006///},
	pages = {105 - 108},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture and microchemistry. The surface topography ultimately affects the nature and the strength of the interactions that occur at biomaterial-biological environment (cell adhesion, mobility, spreading and proliferation). Thus, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (for instance, cells). [1] There are a number of substrates that already have been studied (such as silicone, polystyrene, poly-L-lactic acid and titanium coated polystyrene) in terms of surface topography. [2] However, few studies are related to hydroxyapatite substrates. As it is well established, hydroxyapatite is a well known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense hydroxyapatite substrates was altered by using KFr excimer laser. Excimer lasers produce high-intensity, pulsed ultraviolet radiation and are especially well suited for materials processing due to their large beam cross-section area, which permits using mask projection technologies to process relatively large areas in a single step.[3].},
	keywords = {Bone, cell adhesion, Cells, Ceramic materials, Excimer laser, Excimer lasers, hydroxyapatite, Microtexture, Microtopography, morphology, Strength of materials, Surface modification, Surface topography, Surface treatment},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645413875\&partnerID=40\&md5=0234521c5cb850a0ff51ca4c64c39074},
	author = {Teixeira, S. and Queiroz, A.C. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@book {2486,
	title = {CaO-P                    2O
                    5 Glass-Hydroxyapatite Thin Films Obtained by Laser Ablation: Characterisation and In Vitro Bioactivity Evaluation
},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: mpferraz@ufp.ptReferences: Arias, J.L., (1997) J Mater. Sci. Mater. Med., 8, pp. 873-876;Peraire, C., (1997) 13 th European Conference on Biomaterials, p. 75. , G{\"o}teborg, Sweden, 4-7 Sept; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., Fernandes, M.H., Trigo-Cabral, A., Santos, J.D., Monteiro, F.J., (1999) J. Mat Sci: Mat. Med, 10 (9), p. 567
},
	month = {2004///},
	pages = {347 - 350},
	address = {Porto},
	abstract = {Hydroxyapatite (HA) coatings have been applied to improve adhesion of non-cemented implants to host bone. Plasma spraying is the most common technique leading to thick calcium phosphate films (\&gt;120μm). Pulse laser deposition (PLD), is a possible alternative method to obtain thin (\&lt;10 μm), well adherent hydroxyapatite (HA) films. Similarly to synthetic HA, biological apatites contain Ca                        2+, PO
                        4
                        3- and OH
},
	keywords = {2O, 5 glass, Adhesion, Bioactivity, Bone, Calcium Compounds, CaO-P, Coatings, Deposition, Energy dispersive spectroscopy, Homogeneous mixtures, Hydroxylapatite thin films, Implants (surgical), In vitro bioactivity, Laser ablation, Plasma spraying, Scanning electron microscopy, Thin films, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516195\&partnerID=40\&md5=ddd47b73956669a35aa9041ce9102612},
	author = {Ferraz, M.P. and Monteiro, F.J. and Gi{\~a}o, D. and Leon, B. and Gonzalez, P. and Liste, S. and Serra, J. and Arias, J. and Perez-Amor, M.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@conference {2470,
	title = {"In vitro" bioactivity evaluation of CaO-P                    2O 
                    5 glass-hydroxylapatite thin coatings produced by pulsed laser deposition
},
	booktitle = {Transactions - 7th World Biomaterials CongressTrans. World Biomater. Congr.},
	series = {Transactions - 7th World Biomaterials Congress},
	year = {2004},
	note = {Conference code: 64310Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Arias, J.L.; Depto. de F{\'\i}sica Aplicada, Universidade de Vigo, Lagoas-Marcosende 9, E-36200 Vigo, SpainReferences: Arias, J.L., (1997) J Mater Sci Mater Med, 8, p. 873;Ferraz, M.P., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., (1999) J. Mat Sci Mat Med, 10, p. 567
},
	month = {2004///},
	pages = {1774},
	address = {Sydney},
	abstract = {The synthesis of coatings based on hydroxyapatites (HA) and glass modified hydroxyapatite targets by pulsed laser deposition, was presented. It was observed that coatings produced from HA targets keep their crystallinity through all their thickness, while there is a loss of crystallinity towards the surface of the HA+1.5G coatings. On immersion in SBF for three days, the coatings produced from HA targets suffered a loss of crystallinity near the surfaces, while the crystallinity of the coatings produced from HA+1.5G targets increased. It was also observed that addition of 1.5wt\% of CaO-P                        2O
                        5 glasses to HA thin coatings produced by pulsed laser deposition leads to a significant structural change of the coating surface.
},
	keywords = {Apatites, Bioactivity, Calcium Compounds, Coatings, Crystalline materials, Crystalline phases, hydroxyapatite, Mixtures, Phosphorus Compounds, Pressure effects, Pulsed laser deposition, Scanning electron microscopy, Substrates, Thin coatings, X ray diffraction, X ray photoelectron spectroscopy},
	isbn = {1877040193 (ISBN); 9781877040191 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-13844299568\&partnerID=40\&md5=869dc43fe34d9345a4c3b944a06faad1},
	author = {Arias, J.L. and Liste, S. and Gonz{\'a}lez, P. and Serra, J. and Le{\'o}n, B. and Ferraz, M.P. and Monteiro, F.J. and Giao, D. and P{\'e}rez-Amor, M.}
}
@book {2490,
	title = {Preparation and Characterization of Calcium Phosphate Nanoparticles},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Manuel, C.M.; Inst. de Engenharia Biom{\'e}dica, Lab. de Biomaterials, Universidade do Porto, Rua do Campo Alegre, 823, 4150 -180 Porto, Portugal; email: cmmanuel@fe.up.ptReferences: Manuel, C.M., Ferraz, M.P., Monteiro, F.J., (2003) Key Engineering Materials, pp. 240-242;Jarcho, M., Bolen, C.H., Thomas, M.B., Bobick, J., Doremus, R.H., (1976) J Mat Sci, pp. 2027-2035; 
Jarcho, M., Salsbury, R.L., Thomas, M.B., Doremus, R.H., (1979) J Mat Sci, pp. 142-150; 
Puleo, D.A., Holleran, L.A., Doremus, R.H., Bizios, R., (1991) J Biomed Mat Res, 25, pp. 711-723; 
Fowler, B.O., Moreno, E.C., Brown, W.E., (1966) Arch. Oral Biol., 11, pp. 477-492
},
	month = {2004///},
	pages = {903 - 906},
	address = {Porto},
	abstract = {In this work, CaP nanoparticles were prepared by wet chemical precipitation using three different approaches and were characterised and quantified in terms of their composition. Different precipitating, ageing and drying temperatures were applied and affected HA to TCP ratio. Sintering induced formation of large agglomerates and affected the relative composition, specifically, increased concentrations of TCP as proved by SEM, FTIR and XRD. Different reactants also affected chemical final product composition. Contacts with fibroblasts and osteoblasts revealed better adhesion to sintered substrates.},
	keywords = {Agglomeration, Bioceramics, Biocompatibility, Bone, Calcium Compounds, Cell culture, Cytocompatibility, Fourier transform infrared spectroscopy, hydroxyapatite, nanoparticles, Nanostructured materials, orthopedics, Precipitation (chemical), Scanning electron microscopy, Synthesis (chemical), Tricalcium phosphate, Wet chemical precipitation, X ray diffraction analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346885495\&partnerID=40\&md5=a9f5b3807570c06984809bcb048ffc33},
	author = {Manuel, C.M. and Foster, M. and Monteiro, F.J. and Ferraz, M.P. and Doremus, R.H. and Bizios, R.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2518,
	title = {Synthesis of hydroxyapatite and tricalcium phosphate nanoparticles - Preliminary studies},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {240-242},
	year = {2003},
	note = {Conference code: 60907Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Manuel, C.M.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: candida@ibmc.up.ptReferences: Rhee, S.-H., (2002) Biomaterials, 23, pp. 1147-1152;Yeong, K.C.B., Wang, J., Ng, S.C., (2001) Biomaterials, 22, pp. 2705-2712; 
Fowler, B.O., Moreno, E.C., Brown, W.E., (1966) Arch. Oral Biol., 11, pp. 477-492
},
	month = {2003///},
	pages = {555 - 558},
	address = {Sydney, NSW},
	abstract = {The wet mechanochemical process (MC) and chemical precipitation (CP) used to prepare hydroxyapatite (HA) and tricalcium phosphate (TCP) nanoparticles proved to be successful. In mechanochemical treatment, water and ethanol were used as a solvent but the last one improved particles agglomeration and decreased HA formation. This effect was also observed when aging temperature was decreased in wet chemical precipitation.},
	keywords = {Agglomeration, Calcium Compounds, Chemical precipitation, hydroxyapatite, Mechanochemical treatment, nanoparticles, Nanostructured materials, Precipitation (chemical), Synthesis (chemical), Tricalcium phosphate},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037246970\&partnerID=40\&md5=a4396f71d0ffef576b372e3227b9bb41},
	author = {Manuel, C.M. and Ferraz, M.P. and Monteiro, F.J.},
	editor = {Ben - Nissan B. and Sher D. and Walsh W.}
}
@article {2541,
	title = {Effect of chemical composition on hydrophobicity and zeta potential of plasma sprayed HA/CaO-P                    2O
                    5 glass coatings
},
	journal = {BiomaterialsBiomaterials},
	volume = {22},
	year = {2001},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(01)00059-XPubMed ID: 11603581Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Laborat{\'o}rio de Biomateriais, Inst. de Engenharia Biomedica (INEB), Rua do Campo Alegre 823, 4150 Porto, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: Calcium Compounds; Durapatite, 1306-06-5; lime, 1305-78-8; Oxides; Phosphorus Compounds; phosphorus pentoxide, 1314-56-3; Serum Albumin, BovineReferences: Klein, C.P.A.T., Patka, P., Van der Lubbe, H.B.M., Plasma-sprayed coatings of tetracalciumphosphate, hydroxyl-apatite, and α-TCP on titanium alloy: An interface study (1991) J Biomed Mater Res, 25, pp. 53-65;Jansen, J.A., Van der Waerden, J.P., Wolke, J.G., Histologic investigation of the biologic behavior of different hydroxyapatite plasma-sprayed coatings in rabbits (1993) J Biomed Mater Res, 14, pp. 603-610; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., Comparative biological properties of HA plasma-sprayed coatings having different crystallinities (1994) J Mater Sci: Mater Med, 5, pp. 11-17; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., Comparison of the early production of extracellular matrix on dense hydroxyapatite and hydroxyapatite-coated titanium in cell and organ culture (1995) Biomaterials, 16, pp. 489-495; 
Labat, B., Chanson, A., Frey, J., Effects of γ-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts (1995) J Biomed Mater Res, 29, pp. 1397-1401; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., Adult human bone cells derived from jaw bones cultured on plasma-sprayed or polished surfaces to titanium or hydroxyapatite discs (1996) J Mater Sci: Mater Med, 7, pp. 21-28; 
Silva, P.L., Santos, J.D., Monteiro, F.J., Knowles, J.C., Adhesion and microstructural characterisation of plasma-sprayed hydroxyapatite/glass ceramic coatings onto Ti-6Al-4V substrates (1998) Surf Coating Technol, 102, pp. 191-196; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., CaO-P 2O 5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation (1999) J Biomed Mater Res, 45, pp. 376-383; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced HA plasma-sprayed coatings (1999) J Mater Sci: Mater Med, 10 (9), pp. 567-576; 
Lapin, M., Warocquier-Cl{\'e}rout, R., Legris, C., Degrange, M., Luizard, M.F.S., Correlation between roughness and wettability, cell adhesion and cell migration (1997) J Biomed Mater Res, 36, pp. 99-108; 
Williams, D.F., (1982) Fundamental aspects of biocompatibility, , Boca Raton: CRC Press; 
Adamson, W., (1990) Physical chemistry of surfaces, 5th ed., , New York: Wiley; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions: Structure and properties of the composites (1995) J Mater Sci: Mater Med, 6, pp. 348-352; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass-reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Chen, P., Li, D., Boruvka, L., Rotenberg, Y., Neumann, A.W., Automation of axisymmetric drop shape analysis for measurements of interfacial tensions and contact angles (1990) Coll and Surf, 43, pp. 151-197; 
Cheng, P., Neumann, A.W., Computational evaluation of axisymmetric drop shape analysis-profile (ASA-P) (1992) Coll and Surf, 62, pp. 297-306; 
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Schrader, M.E., Young Dupr{\'e} revisited (1995) Langmuir, 11, p. 3585; 
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Williams, R.L., Williams, D.F., Albumin adsorption on metal surfaces (1988) Biomaterials, 9, pp. 206-212; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tension, and zeta potential measurements of glass-reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Tripp, B.C., Magda, J.J., Andrade, J.D., Adsorption of globular proteins at the air/water interface as measured via dynamic surface tension: Concentration dependence, mass-transfer and adsorption kinetics (1995) J Coll Interface Sci, 173, pp. 16-27; 
Voigt, A., Thiel, O., Williams, D., Policova, Z., Zing, W., Neumann, A.W., Axisymmetric drop shape analysis (ADSA) applied to protein solutions (1991) Coll and Surf, 58, pp. 315-326; 
Suzuki, T., Nishizawa, K., Yokogawa, Y., Kameyama, T., Time-dependent variation of the surface structure of bioceramics in tissue culture medium and the effect of adhesiveness of cells (1996) J Ferment Bioeng, 81, pp. 226-232; 
Susuki, T., Yamamoto, T., Yoriyama, M., Kameyama, T., Surface instability of calcium phosphate ceramics in tissue culture medium and the effect on adhesion and growth of anchorage-dependent animal cells (1997) J Biomed Mater Res, 34, pp. 504-517; 
Haynes, C.A., Norde, W., Globular proteins at solid/liquid interfaces (1994) Coll and Surf B: Biointerfaces, 2, pp. 517-566; 
Kondori, K., Soito, M., Takebe, T., Yasukawa, A., Adsorption of bovine serum albumin on synthetic carbonate calcium hydroxyapatite (1995) J Coll Interface Sci, 184, pp. 124-129
},
	month = {2001///},
	pages = {3105 - 3112},
	abstract = {Multilayered plasma sprayed coatings on the surface of Ti-6Al-4V alloys have been prepared, which were composed of an underlayer of HA and a surface layer of a CaO-P                        2O
                        5 glass-HA composite, with 2 or 4 wt\% of glass. Contact angle and surface tension variation with time, for both water and a protein solution, were determined by the sessile and pendent drop methods respectively using the ADSA-P software. Wettability studies showed that hydrophobicity of the coatings increase with the glass addition. The work of adhesion of albumin was also altered in a controlled manner by the addition of the CaO-P
                        2O
                        5 glass, being lower on the composite coatings than on HA. Zeta potential (ZP) results showed that composite coatings presented a higher net negative charge than HA coatings and that ZP values were also influenced by the content of the glass. This study demonstrated that the surface properties of those coatings may be modified by the addition of CaO-P
                        2O
                        5 glass. {\textcopyright} 2001 Published by Elsevier Science Ltd.
},
	keywords = {Adhesion, alloy, analytic method, article, bovine serum albumin, Calcium Compounds, calcium oxide, chemical composition, Chemical modification, Coatings, Composition, computer program, Contact angle, Durapatite, glass, hydrophobicity, hydroxyapatite, Hydroxyapatite glass composites, oxide, Oxides, pentoxide, Phosphorus Compounds, priority journal, protein, Serum Albumin, Bovine, Sprayed coatings, Surface charge, Surface Properties, Surface tension, Surface tension zeta potential, time, unclassified drug, Water, wettability, Wetting, X-Ray Diffraction, Zeta potential},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034841180\&partnerID=40\&md5=552d64a5e7743f1d3c19fafc42a6e38d},
	author = {Ferraz, M.P. and Monteiro, F.J. and Serro, A.P. and Saramago, B. and Gibson, I.R. and Santos, J.D.}
}
@article {2543,
	title = {HA and double-layer HA-P                    2O
                    5/CaO glass coatings: Influence of chemical composition on human bone marrow cells osteoblastic behavior
},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {12},
	year = {2001},
	note = {Cited By (since 1996): 18Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1011245828046Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Rua Dr. Manuel Pereira da Silva, 4200 Porto, PortugalReferences: Klein, C.P.A.T., Patka, P., Van der Lubbe, H.B.M., (1991) J. Biomed. Mater. Res., 25, p. 53;Jansen, J.A., Van Der Waerden, J.P.C.M., Wolke, J.G.C., (1993) J. Mater. Sci Mater. Med., 4, p. 466; 
Frayssinet, P., Tourenne, F., Rouquet, P., Conte, P., Delga, C., Bonel, G., (1994) J. Mater. Sci. Mater. Med., 5, p. 11; 
Courteney-Harris, R.G., Kayser, M.V., Downes, S., (1995) Biomaterials, 16, p. 489; 
Labat, B., Chanson, A., Frey, J., (1995) J. Biomed. Mater. Res., 29, p. 1397; 
De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., (1996) J. Mater. Sci. Mater. Med., 7, p. 21; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., (1999) J. Mater. Sci: Mater. Med., 10, p. 567; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mat. Res., 45, p. 376; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res., 47, p. 603; 
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Best, S., Sim, B., Kayser, M., Downes, S., (1997) J. Mater. Sci. Mater. Med., 8; 
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Gundle, R., Joyner, C.Y., Triffit, J.T., (1995) Bone, 16, p. 597; 
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Martin, T.J., Findlay, D.M., Heath, J.K., Ng, K.W., (1993) Handbook of Experimental Pharmacology, p. 149. , edited by J. R. Mundy and T. J. Martin (Springer-Verlag, Berlin); 
Stein, G.S., Lian, J.B., (1993) Cellular and Molecular Biology of Bone, p. 47. , edited by M. Noda (Academic Press Inc., Tokyo); 
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Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., J. Biomed. Mater. Res., , in press
},
	month = {2001///},
	pages = {629 - 638},
	abstract = {Human osteoblastic bone marrow derived cells were cultured for 28 days onto the surface of a glass reinforced hydroxyapatite (HA) composite and a commercial type HA plasma sprayed coatings, both in the "as-received" condition and after an immersion treatment with culture medium during 21 days. Cell proliferation and differentiation were analyzed as a function of the chemical composition of the coatings and the immersion treatment. Cell attachment, growth and differentiation of osteoblastic bone marrow cells seeded onto "as-received" plasma sprayed coatings were strongly affected by the time-dependent variation of the surface structure occurring during the first hours of culture. Initial interactions leading to higher amounts of adsorbed protein and zeta potential shifts towards negative charges appeared to result in surface structures with better biological performance. Cultures grown onto the pretreated coatings showed higher rate of cell proliferation and increased functional activity, as compared to those grown onto the corresponding "as-received" materials. However, the cell behavior was similar in the glass composite and HA coatings. The results showed that the glass composites present better characteristics for bone cell growth and function than HA. In addition, this work also provide evidence that the biological performance of the glass composites can be modulated and improved by manipulations in the chemical composition, namely in the content of glass added to HA. {\textcopyright} 2001 Kluwer Academic Publishers.},
	keywords = {Adsorption, alkaline phosphatase, article, Biomedical engineering, Bone, bone marrow, bone marrow cell, Calcium, calcium oxide, cell adhesion, Cell culture, cell differentiation, cell growth, cell proliferation, chemical composition, Coatings, controlled study, culture medium, enzyme activity, glass, Growth kinetics, human, human cell, hydroxyapatite, immersion, osteoblast, oxygen, performance, phosphorus, priority journal, protein binding, protein content, Proteins, Scanning electron microscopy, Surface charge, surface property, time, X ray diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034918167\&partnerID=40\&md5=3783f798f6eea84dfff83e16a393fe54},
	author = {Ferraz, M.P. and Fernandes, M.H. and Santos, J.D. and Monteiro, F.J.}
}
@article {2548,
	title = {Histological studies of double layer HA/CaO-P                    2O
                    5 glass plasma sprayed coatings using Rabbit model
},
	journal = {Key Engineering MaterialsKey Eng Mat},
	volume = {192-195},
	year = {2001},
	note = {Conference code: 57760Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; Instituto de Engenharia Biomedica, (INEB), Porto, PortugalReferences: Lacefield, W.R., (1988) Bioceramics: Material Characteristics Versus In Vivo Behaviour, pp. 72-80. , P. Ducheyne, J.E. Lemons. New York: New York Academy of Science Publishers;Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., (1994) J Biomed Mater Res, 28, pp. 961-967; 
Klein, C.P.A.T., Wolke, J.G.C., De Blieck-Hogervorst, J.M.A., De Groot, K., (1994) J Biomed Mater Res, 28, pp. 909-917; 
Burnie, J., Gilchrist, T., (1983) Ceramic in Surgery, pp. 169-176. , P. Vicenzini. Amsterdam: Elsevier Science Publishers; 
Ferraz, M.P., Fernandes, M.H., Trigo Cabrai, A., Santos, J.D., Monteiro, F.J., (1999) J Mater Sci Mater Med, 10 (9), pp. 567-576; 
Oonishi, H., Hench, L.L., Wilson, J., Sugihara, F., Tsuji, E., Kushitani, S., Iwak, H., (1999) J Biomed Mater Res, 44, pp. 31-43; 
Kitsugi, T., Nakamura, T., Oka, M., Senaha, Y., Got, T., (1996) J Biomed Mater Res, 30, pp. 261-269; 
Klein, C.P.A.T., Patka, P., Van Der Lubbe, H.B.M., Wolke, J.G.V., De Groot, K., (1991) J Biomed Mater Res, 25, pp. 53-65
},
	month = {2001///},
	pages = {449 - 452},
	publisher = {Trans Tech Publ},
	address = {Bologna, Italy},
	abstract = {The in vivo biological performance of a double layer HA/CaO-P                        2O
                        5 glass and HA plasma sprayed coatings was assessed, using cylindrical implants that were placed in rabbits tibiae and histologically characterised after 2, 4, 8 and 16 weeks of implantation. For both types of coatings, long-term histological results indicated successful contact between implanted materials and bone, with organised new bone established at the interface. The main difference between the two coatings corresponded to the shorter implantation periods, where the composite coating induced faster osteointegration than HA coating. The faster formation of organised bone for composite coatings is discussed in terms of the presence of phase composition in the coatings structure.
},
	keywords = {Bond strength (materials), Bone, Double layer coatings, hydroxyapatite, Implants (surgical), In vivo evaluation, Interfaces (materials), morphology, Phase composition, Phosphates, Plasma sprayed coatings, Rabbit model, Scanning electron microscopy, Sprayed coatings, X ray diffraction analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035147691\&partnerID=40\&md5=1857cde1f297ab045f2369691bdbdc8c},
	author = {Ferraz, M.P. and Santos, J.D. and Afonso, A. and Vasconcelos, M. and Monteiro, F.J.}
}
@article {2561,
	title = {Flow cytometry analysis of the effects of pre-immersion on the biocompatibility of glass-reinforced hydroxyapatite plasma-sprayed coatings},
	journal = {BiomaterialsBiomaterials},
	volume = {21},
	year = {2000},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(99)00249-5PubMed ID: 10721750Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University College London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United Kingdom; email: iolsen@eastman.ucl.ac.ukChemicals/CAS: Biocompatible Materials; bone sialoprotein; Coated Materials, Biocompatible; Durapatite, 1306-06-5; Fibronectins; SialoglycoproteinsManufacturers: Plasma Biotal, United KingdomReferences: Passuti, N., Daculsi, G., Martin, S., Macroporous calcium phosphate ceramics for long bone surgery in human and dogs - Clinical and histological studies (1990) Clinical Implant Materials, pp. 255-258. , V. Soltesz, \& A. Lee. Amsterdam: Elsevier Science Publishers;Legeros, R., Calcium phosphate materials in restorative dentistry (1988) A Review. Adv Dent Res, 2, pp. 164-180; 
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De Santis, D., Guerriero, C., Nocini, P.F., Ungersbock, A., Richards, G., Gotte, P., Armato, U., Adult human bone cells derived from jaw bones cultured on plasma-sprayed or polished surfaces to titanium or hydroxyapatite discs (1996) J Mater Sci: Mater Med, 7, pp. 21-28; 
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Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced HA plasma sprayed coatings (1999) J Mater Sci: Mater Med, 10, pp. 567-576; 
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Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of the effects of glass on the biocompatibility of plasma-sprayed hydroxyapatite/CaO-P 2O 5 coatings J Biomed Mater Res, , in press; 
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},
	month = {2000///},
	pages = {813 - 820},
	publisher = {Elsevier Science Ltd},
	abstract = {Multilayered coatings composed of mixtures of HA and P                        2O
                        5-based bioactive glasses are of potential clinical benefit in orthopaedic and dental surgery. Pre-immersion of these materials has been reported to further enhance their efficacy in vivo, although the precise biological effects of this treatment are not yet known. In this study we have therefore prepared double-layer plasma-sprayed coatings and evaluated the effects of pre-immersion on the growth and function of human osteosarcoma cells in vitro, using the MTT assay and flow cytometry analysis, respectively. The results showed that the increase in numbers of viable cells was the same or elevated following incubation on the pre-immersed HA and glass-reinforced HA coatings compared with the non-immersed materials. In addition, the expression of bone sialoprotein and fibronectin, two key connective tissue antigens, was up-regulated in cultures grown on the pre-immersed surfaces compared with the non-treated materials. Moreover, cell numbers and antigen expression both improved as the proportion of glass increased, particularly in the pre-immersed samples. Our findings thus suggest that the immersion treatment of these materials appeared to improve the response of these bone-like cells. Copyright (C) 2000 Elsevier Science Ltd.
Multilayered coatings composed of mixtures of HA and P
                        2O
                        5-based bioactive glasses are of potential clinical benefit in orthopaedic and dental surgery. Pre-immersion of these materials has been reported to further enhance their efficacy in vivo, although the precise biological effects of this treatment are not yet known. In this study we have therefore prepared double-layer plasma-sprayed coatings and evaluated the effects of pre-immersion on the growth and function of human osteosarcoma cells in vitro, using the MTT assay and flow cytometry analysis, respectively. The results showed that the increase in numbers of viable cells was the same or elevated following incubation on the pre-immersed HA and glass-reinforced HA coatings compared with the non-immersed materials. In addition, the expression of bone sialoprotein and fibronectin, two key connective tissue antigens, was up-regulated in cultures grown on the pre-immersed surfaces compared with the non-treated materials. Moreover, cell numbers and antigen expression both improved as the proportion of glass increased, particularly in the pre-immersed samples. Our findings thus suggest that the immersion treatment of these materials appeared to improve the response of these bone-like cells.
},
	keywords = {antigen expression, article, Bioactive glasses, Biocompatibility, Biocompatible Materials, Biomaterials, Cell culture, Cell Division, cell growth, cell proliferation, Cell size, Coated Materials, Biocompatible, controlled study, cytology, Cytoplasmic Granules, Dental Materials, drug manufacture, Durapatite, Fibronectin, Fibronectins, flow cytometry, Flow cytometry analysis, glass, Glass fibers, Glass-reinforced hydroxyapatite, Growth kinetics, human, human cell, Humans, hydroxyapatite, in vitro study, materials testing, orthopedics, Osteosarcoma, osteosarcoma cell, Phosphates, Plasma spraying, Pre-immersion, priority journal, protein expression, Proteins, Sialoglycoproteins, sialoprotein, Sprayed coatings, Surface Properties, technique, Tumor Cells, Cultured},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034004660\&partnerID=40\&md5=4f46f4bb4619ff65dccbfcc0fdcd03b1},
	author = {Ferraz, M.P. and Knowles, J.C. and Olsen, I. and Monteiro, F.J. and Santos, J.D.}
}
@article {2574,
	title = {CaO-P                    2O
                    5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation
},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {45},
	year = {1999},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19990615)45:4<376::AID-JBM13>3.0.CO;2-SLanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; Departamento Engenharia Metalurgica, FEUP, Universidade do Porto, R. dos Bragas, 4099 Porto Codex, PortugalChemicals/CAS: Alloys; Calcium, 7440-70-2; CaO-SiO2-P2O5-CaF2 glass; Coated Materials, Biocompatible; Durapatite, 1306-06-5; Phosphates; Powders; Resin Cements; Solutions; titanium alloy (TiAl6V4), 12743-70-3; Titanium, 7440-32-6References: Denissen, H., Mangano, C., Venini, G., (1985) Hydroxylapatite Implants., , Padua: Piccin Nuova Libraria, S.P.A;Van Raemdonck, W., Ducheyne, P., De Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Biomaterials, pp. 143-166. , Ducheyne P, Hastings GW, editors, Boca Raton, FL: CRC Press; 
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Verhoeven, J.A.T., Van Doveren, H., An XPS investigation of the interface of methane, ethyne, ethene and ethane with barium surface (1982) Surf Sci, 123, pp. 369-383
},
	month = {1999///},
	pages = {376 - 383},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Double-layer composite coatings composed of a P                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
Double-layer composite coatings composed of a P
                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
},
	keywords = {alloy, Alloys, Apatite, article, Atomic absorption spectroscopy, Atomic spectroscopy, Bioactivity, Biocompatibility, biomaterial, Biomaterials, Biomedical engineering, Body fluids, Calcium, Calcium Compounds, CaO SiO2 P2O5 CaF2 glass, CaO-SiO2-P2O5-CaF2 glass, Chemical analysis, chemical composition, Coated Materials, Biocompatible, Double layer coatings, Double-layer coatings, Durapatite, film, glass, Glass plasma spraying, human, Humans, hydroxyapatite, immersion, implant, in vitro study, Ionic solution changes, Magnesium, materials testing, Molybdenum blue, Multilayers, Particle Size, phosphate, Phosphate minerals, Phosphates, Phosphorus Compounds, phosphorus pentoxide, Plasma spraying, potassium, powder, Powders, resin cement, Resin Cements, Scanning electron microscopy, Sodium, solution and solubility, Solutions, structure analysis, Substrates, Surface Properties, surface property, thickness, Tissue, Titanium, titanium alloy (TiAl6V4), Titanium alloys, X ray photoelectron spectroscopy},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033563315\&partnerID=40\&md5=8c0de1e25709302641b203efac50337b},
	author = {Ferraz, M.P. and Monteiro, F.J. and Santos, J.D.}
}
@article {2571,
	title = {Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P                    2O
                    5 coatings
},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {47},
	year = {1999},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19991215)47:4<603::AID-JBM18>3.0.CO;2-6PubMed ID: 10497297Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University of London, 256 Gray{\textquoteright}s Inn Road, London WC1X 8LD, United KingdomChemicals/CAS: Bone Substitutes; Coated Materials, Biocompatible; HydroxyapatitesReferences: Passuti, N., Daculsi, G., Martin, S., Macroporous calcium phosphate ceramics for long bone surgery in human and dogs - Clinical and histological studies (1990) Clinical Implant Materials, pp. 255-258. , Soltesz V, Lee A, editors. Amsterdam: Elsevier Sciesnce Publishers;Legeros, R., Calcium phosphate materials in restorative dentistry. A review (1988) Adv Dent Res, 2, pp. 164-180; 
Hench, L.L., Bioactive ceramics: From concept to clinic (1992) J Am Ceram Soc, 74, pp. 1487-1510; 
Conish, H., Aoki, H., Sawai, K., (1981) Science and Medical Applications of Hydroxyapatite, , Tokyo: Takyama Press System Centre Co; 
Raemdonick, W., Ducheyne, P., Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Materials, pp. 143-166. , Ducheyne P, Hastings GW, editors. Boca Raton, FL: CRC Press; 
Rey, C., Freche, M., Heughebaert, M., Vignoles, M., Apatite chemistry in biomaterial preparation, shaping and biological behaviour (1991) 4th Symposium on Ceramics in Medicine - Bioceramics, 4, pp. 57-64. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Nagase, M., Abe, Y., Udagwa, E., Toxicity of silica-containing calcium phosphate glasses demonstrated in mice (1992) Biomaterials, 13, pp. 172-175; 
Burnie, J., Gilchrist, T., Controlled released glass - A new biomaterial (1983) Ceramics in Surgery, pp. 169-176. , Vicenzini P, editor. Amsterdam: Elsevier Science Publishers; 
Jha, L.J., Santos, J.D., Knowles, J.C., Characterization of apatite layer formation on P 2O 5-CaO, P 2O 5-CaO-Na 2O, P 2O 5-CaO-Na 2O-Al 2O 3 glass hydroxyapatite composites (1996) J Biomed Mater Res, 31, pp. 481-486; 
Rawlings, R.D., Rogers, P.S., Stokes, P.M., Glass-ceramics containing apatite (1987) High Tech Ceramics, pp. 73-82. , Vicenzini P, editor. Amsterdam: Elsevier Science Publishers; 
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Ferraz, M.P., Monteiro, F.J., Santos, J.D., In vitro bioactivity and osteocompatibility evaluation of plasma sprayed hydroxyapatite/glass composite coatings onto Ti-6AL-4V substrates (1997) 9th International Conference on Biomedical Engineering, pp. 557-559. , Goh JCH, editor. Singapore: BAC Printers; 
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Lopes, M.A., Knowles, J.C., Hing, K.A., Santos, J.D., Monteiro, F.J., Olsen, I., Biological evaluation of glass reinforced hydroxyapatite by flow cytometry (1997) 10th Symposium on Ceramics in Medicine-bioceramics, 10, pp. 575-578. , Sedel L, Rey C, editors. Cambridge, UK: University Press; 
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Sandy, J., Davies, M., Prime, S., Farndale, R., Signal pathways that transduce growth factor-stimulated mitogenesis in bone cells (1998) Bone, 23, pp. 17-26
},
	month = {1999///},
	pages = {603 - 611},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Multilayered coatings composed of mixtures of hydroxyapatite (HA) and P                        2O
                        5-based bioactive glasses offer potential clinical benefits in orthopedic and dental surgery. In this study double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The cells were cultured on the materials and the effects on cell growth were determined using a spectrophometric assay of a mitochondrial enzyme that is active in viable cells. While none of the materials influenced the growth of the MG63 cells, the HOS cells appeared to undergo less proliferation on all the HA materials. Flow cytometry analysis was carried out using rabbit antibodies against osteonectin, osteopontin, bone sialoprotein, fibronectin, and collagen type I to measure the effects of the materials on key cellular functions. The results showed that the materials downregulated the expression of these extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells.
In this study, double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The materials downregulated the expression of the extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells.
},
	keywords = {Animals, Antibodies, antigen expression, article, Bioassay, Biomaterials, Bone cells, Bone sialoprotein, Bone Substitutes, calcium phosphate, cancer cell culture, Cell Division, cell function, cell granule, cell growth, cell proliferation, Cell size, Cells, Coated Materials, Biocompatible, Collagen, collagen type 1, connective tissue, controlled study, dentistry, enzyme activity, Enzymes, Fibronectin, flow cytometry, Flow cytometry analysis, glass, Growth kinetics, human, human cell, Humans, hydroxyapatite, Hydroxyapatite-glass, Hydroxyapatites, Incubation, Mitochondrial enzyme, orthopedics, Osteoblasts, osteonectin, Osteopontin, Osteosarcoma, osteosarcoma cell, Osteosarcoma cells, Phosphates, Proliferation, Proteins, Rabbits, sialoprotein, Spectrophometric assay, Tissue culture, Tumor Cells, Cultured},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031573504\&partnerID=40\&md5=c6bbcda19f6e76323d16484a9db1c0da},
	author = {Ferraz, M.P. and Knowles, J.C. and Olsen, I. and Monteiro, F.J. and Santos, J.D.}
}
@article {2573,
	title = {In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {10},
	year = {1999},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1008924516146Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Institute of Biomedical Engineering, Faculties of Dental Medicine, Universidade do Porto, 4200 Porto, PortugalChemicals/CAS: alkaline phosphatase, 9001-78-9; calcium ion, 14127-61-8; hydroxyapatite, 1306-06-5, 51198-94-8; phosphorus, 7723-14-0; protein, 67254-75-5References: Passuti, N., Daculsi, G., Martin, S., (1990) Clinical Implant Materials, p. 255. , edited by V. Soltesz and A. Lee (Elsevier, Amsterdam);Legeros, R., (1988) Adv. Dental Res., 2, p. 164; 
Hench, L.L., (1992) J. Amer. Ceram. Soc., 74, p. 1487; 
Kokubo, T., (1993) Bone-Bonding Biomaterals, p. 31. , edited by P. Ducheyne, T. Kokubo and C. A. Blitterswijk (Reed Health-Care Comunications, Leiden, The Netherlands); 
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Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , JAAS; 
Raemdonck, W., Ducheyne, P., Meester, P., (1984) Metal and Ceramic Materials, p. 143. , edited by P. Ducheyne and G. W. Hastings (CRC Press, Boca Raton, FL); 
Rey, C., Freche, M., Heughebaert, M., Vignoles, M., (1991) Bioceramics 4, p. 57. , edited by W. Bonfield, G. W. Hastings and K. E. Tanner (Butterworth-Heinemann, London); 
Nagase, M., Abe, Y., Udagawa, E., (1992) Biomaterials, 13, p. 172; 
Burnie, J., Gilchrist, T., (1993) Ceramics in Surgery, p. 169. , edited by P. Vincenzini (Elsevier, Amsterdam); 
Burnie, F., Gilchrist, T., Ceramics in Surgery, p. 177; 
Rawlings, R.D., Rogers, P.S., Stokes, P.M., (1987) High Tech Ceramics, p. 73. , edited by P. Vincenzini (Elsevier, Amsterdam); 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) Biomaterials, 15, p. 5; 
Santos, J.D., Lakhan, J.J., Monteiro, F.J., (1995) Biomaterials, 16, p. 521; 
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},
	month = {1999///},
	pages = {567 - 576},
	publisher = {Kluwer Academic Publishers},
	abstract = {Human osteoblastic bone marrow cells were cultured far periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the {\textquoteright}as-received{\textquoteright} condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorus in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the {\textquoteright}as-received{\textquoteright} HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, {\textquoteright}as-received{\textquoteright} HA hardly supported cell growth. Previously immersed material samples showed cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow eel Is was closer to that observed in control cultures.Human osteoblastic bone marrow cells were cultured for periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the {\textquoteright}as-received{\textquoteright} condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorous in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the {\textquoteright}as-received{\textquoteright} HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, {\textquoteright}as-received{\textquoteright} HA hardly supported cell growth. Previously immersed material samples cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow cells was closer to that observed in control cultures.
},
	keywords = {adult, alkaline phosphatase, article, Bone, bone marrow cell, Bone replacement materials, Calcium, calcium ion, case report, Cell culture, cell differentiation, cell growth, Cells, controlled study, culture medium, enzyme activity, Enzyme kinetics, glass, Growth kinetics, human, Human bone marrow cell, human cell, hydroxyapatite, male, osteoblast, Phosphates, phosphorus, priority journal, protein, protein determination, Proteins, scanning electron microscope, Scanning electron microscopy, Sprayed coatings},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033198788\&partnerID=40\&md5=ed28cf28c5da19665b6a3c3c916dad56},
	author = {Ferraz, M.P. and Fernandes, M.H. and Cabral, A.T. and Santos, J.D. and Monteiro, F.J.}
}
