



@article {3275,
	title = {Biodistribution and pharmacokinetics of Dapivirine-loaded nanoparticles after vaginal delivery in mice},
	journal = {Pharmaceutical Research},
	volume = {31},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1834 - 1845},
	abstract = {Purpose: To assess the potential of polymeric nanoparticles (NPs) to affect the genital distribution and local and systemic pharmacokinetics (PK) of the anti-HIV microbicide drug candidate dapivirine after vaginal delivery. Methods: Dapivirine-loaded, poly(ethylene oxide)-coated poly(epsilon-caprolactone) (PEO-PCL) NPs were prepared by a nanoprecipitation method. Genital distribution of NPs and their ability to modify the PK of dapivirine up to 24 h was assessed after vaginal instillation in a female mouse model. Also, the safety of NPs upon daily administration for 14 days was assessed by histological analysis and chemokine/cytokine content in vaginal lavages. Results: PEO-PCL NPs (180-200 nm) were rapidly eliminated after administration but able to distribute throughout the vagina and lower uterus, and capable of tackling mucus and penetrate the epithelial lining. Nanocarriers modified the PK of dapivirine, with higher drug levels being recovered from vaginal lavages and vaginal/lower uterine tissues as compared to a drug suspension. Systemic drug exposure was reduced when NPs were used. Also, NPs were shown safe upon administration for 14 days. Conclusions: Dapivirine-loaded PEO-PCL NPs were able to provide likely favorable genital drug levels, thus attesting the potential value of using this vaginal drug delivery nanosystem in the context of HIV prophylaxis. {\textcopyright} 2014 Springer Science+Business Media New York.},
	keywords = {HIV/AIDS, microbicides, poly(ε-caprolactone), pre-exposure prophylaxis, vaginal drug delivery},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904252215\&partnerID=40\&md5=e50091fa44bc3ac794251baa9e57d736},
	author = {Das Neves, J. and Ara{\'u}jo, F. and Andrade, F. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2846,
	title = {Assessing the physical-chemical properties and stability of dapivirine-loaded polymeric nanoparticles},
	journal = {International Journal of Pharmaceutics},
	volume = {456},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {307 - 314},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84884308158\&partnerID=40\&md5=52f91c19cbe922064bbdc786298301a0},
	author = {Das Neves, J. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2857,
	title = {In vitro and Ex Vivo evaluation of polymeric nanoparticles for vaginal and rectal delivery of the anti-HIV drug dapivirine},
	journal = {Molecular Pharmaceutics},
	volume = {10},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {2793 - 2807},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879736868\&partnerID=40\&md5=cd7771212c94b7031331e338743b05ed},
	author = {Das Neves, J. and Ara{\'u}jo, F. and Andrade, F. and Michiels, J. and Ari{\"e}n, K.K. and Vanham, G. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2181,
	title = {Interactions of microbicide nanoparticles with a simulated vaginal fluid},
	journal = {Molecular PharmaceuticsMol. Pharm.},
	volume = {9},
	year = {2012},
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},
	month = {2012///},
	pages = {3347 - 3356},
	abstract = {The interaction with cervicovaginal mucus presents the potential to impact the performance of drug nanocarriers. These systems must migrate through this biological fluid in order to deliver their drug payload to the underlying mucosal surface. We studied the ability of dapivirine-loaded polycaprolactone (PCL)-based nanoparticles (NPs) to interact with a simulated vaginal fluid (SVF) incorporating mucin. Different surface modifiers were used to produce NPs with either negative (poloxamer 338 NF and sodium lauryl sulfate) or positive (cetyltrimethylammonium bromide) surface charge. Studies were performed using the mucin particle method, rheological measurements, and real-time multiple particle tracking. Results showed that SVF presented rheological properties similar to those of human cervicovaginal mucus. Analysis of NP transport indicated mild interactions with mucin and low adhesive potential. In general, negatively charged NPs underwent subdiffusive transport in SVF, i.e., hindered as compared to their diffusion in water, but faster than for positively charged NPs. These differences were increased when the pH of SVF was changed from 4.2 to 7.0. Diffusivity was 50- and 172-fold lower in SVF at pH 4.2 than in water for negatively charged and positively charged NPs, respectively. At pH 7.0, this decrease was around 20- and 385-fold, respectively. The estimated times required to cross a layer of SVF were equal to or lower than 1.7 h for negatively charged NPs, while for positively charged NPs these values were equal to or higher than 7 h. Overall, our results suggest that negatively charged PCL NPs may be suitable to be used as carriers in order to deliver dapivirine and potentially other antiretroviral drugs to the cervicovaginal mucosal lining. Also, they further reinforce the importance in characterizing the interactions of nanosystems with mucus fluids or surrogates when considering mucosal drug delivery. {\textcopyright} 2012 American Chemical Society.},
	keywords = {microbicides, mucin, mucoadhesion, mucosal drug delivery, particle tracking, rheology},
	isbn = {15438384 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868526145\&partnerID=40\&md5=bd161f1b2ce30fba31c7a482dbe60b86},
	author = {Das Neves, J. and Rocha, C.M.R. and Gon{\c c}alves, M.P. and Carrier, R.L. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2274,
	title = {Characterization of polymeric solutions as injectable vehicles for hydroxyapatite microspheres},
	journal = {AAPS PharmSciTechAAPS PharmSciTech},
	volume = {11},
	year = {2010},
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Xu, H.H., Weir, M.D., Burguera, E.F., Fraser, A.M., Injectable and macroporous calcium phosphate cement scaffold (2006) Biomat, 27 (24), pp. 4279-4287; 
Gisep, A., Curtis, R., Hanni, M., Suhm, N., Augmentation of implant purchase with bone cements: An in vitro study of injectability and dough distribution (2006) J. Biomed. Mater. Res. B-Appl. Biomat., 77 (1), pp. 114-119; 
Krebs, J., Ferguson, S.J., Bohner, M., Baroud, G., Steffen, T., Heini, P.F., Clinical measurements of cement injection pressure during vertebroplasty (2005) Spine, 30 (5), pp. E118-E122; 
Draget, K.I., Skjak-Braek, G., Smidsrod, O., Alginate-based new materials (1997) Int. J. Biol. Macromol., 21 (1-2), pp. 47-55
},
	month = {2010///},
	pages = {852 - 858},
	abstract = {A polymeric solution and a reinforcement phase can work as an injectable material to fill up bone defects. However, the properties of the solution should be suitable to enable the transport of that extra phase. Additionally, the use of biocompatible materials is a requirement for tissue regeneration. Thus, we intended to optimize a biocompatible polymeric solution able to carry hydroxyapatite microspheres into bone defects using an orthopedic injectable device. To achieve that goal, polymers usually regarded as biocompatible were selected, namely sodium carboxymethylcellulose, hydroxypropylmethylcellulose, and Na-alginate (ALG). The rheological properties of the polymeric solutions at different concentrations were assessed by viscosimetry before and after moist heat sterilization. In order to correlate rheological properties with injectability, solutions were tested using an orthopedic device applied for minimal invasive surgeries. Among the three polymers, ALG solutions presented the most suitable properties for our goal and a non-sterile ALG 6\% solution was successfully used to perform preliminary injection tests of hydroxyapatite microspheres. Sterile ALG 7.25\% solution was found to closely match non-sterile ALG 6\% properties and it was selected as the optimal vehicle. Finally, sterile ALG 7.25\% physical stability was studied at different temperatures over a 3-month period. It was observed that its rheological properties presented minor changes when stored at 25{\textdegree}C or at 4{\textdegree}C. {\textcopyright} 2010 American Association of Pharmaceutical Scientists.},
	keywords = {Alginate, alginic acid, article, Biocompatibility, Biocompatible Materials, Capsules, Carboxymethylcellulose, Drug Compounding, Durapatite, Excipients, hydroxyapatite, Hydroxypropylmethylcellulose, Injectability, injection, Injections, instrument sterilization, microsphere, Microspheres, minimally invasive surgery, molecular weight, orthopedic equipment, Polymers, priority journal, Solutions, Vehicles, viscometry},
	isbn = {15309932 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77954819260\&partnerID=40\&md5=e8503462fa39dcc285aef7e07f765eef},
	author = {Oliveira, S.M. and Almeida, I.F. and Costa, P.C. and Barrias, C.C. and Ferreira, M.R.P. and Bahia, M.F. and Barbosa, M.A.}
}
@book {2320,
	title = {Morphology and mechanical properties of injectable ceramic microspheres},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {396-398},
	year = {2009},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAEdoi: 10.4028/0-87849-353-0.691Language of Original Document: EnglishCorrespondence Address: Oliveira, S. M.; ESTV, Departamento de Engenharia Mec{\^a}nica, Instituto Polit{\'e}cnico de Viseu, Viseu, PortugalReferences: Barrias, C.C., Ribeiro, C.C., Lamghari, M., Miranda, C.S., Barbosa, M.A., Proliferation, activity, and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) Journal of Biomedical Materials Research, 72 A (1), pp. 57-66;Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Bioceramics, 16, pp. 254-262,877-880; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25 (18), pp. 4363-4373; 
Silva, R.V., Camilli, J.A., Bertran, C.A., Moreira, N.H., The use of hydroxyapatite and autogenous cancellous bone grafts to repair bone defects in rats (2005) International Journal of Oral and Maxillofacial Surgery, 34 (2), pp. 178-184; 
Boyan, B.D., Lossd{\"o}rfer, S., Wang, L., Zhao, G., Lohmann, C.H., Cochran, D.L., Schwartz, Z., Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies (2003) Eur Cell Mater, 6, pp. 22-27; 
Cardoso, O.N., Sotto Mayor, T., Pinto, A.M.F.R., Campos, J.B.L.M., Axial dispersion of particles in a slugging column - the role of the laminar wake of the bubbles (2003) Chemical Engineering Science, 58 (18), pp. 4159-4172; 
Gauthier, O., Bouler, J.M., Weiss, P., Bosco, J., Aguado, E., Daculsi, G., Short-term effects of mineral particle sizes on cellular degradation activity after implantation of injectable calcium phosphate biomateriais and the consequences for bone substitution (1999) Bone, 25 (2), pp. 71S-74S; 
Xu, H.H., Weir, M.D., Burguera, E.F., Fraser, A.M., Injectable and macroporous calcium phosphate cement scaffold (2006) Biomaterials, 27 (24), pp. 4279-4287; 
Ginebra, M.P., Rilliard, A., Fernandez, E., Elvira, C., San Roman, J., Planell, J.A., Mechanical and rheological improvement of a calcium phosphate cement by the addition of a polymeric drug (2001) J Biomed Mater Res, 57 (1), pp. 113-118
},
	month = {2009///},
	pages = {691 - 694},
	abstract = {The aim of this study was to analyze the effect of starting powder granulometry and sintering conditions on the morphological structure and mechanical properties of injectable hydroxyapaptite (HAp) microspheres. The mechanical properties of the microspheres were evaluated, to investigate if their integrity could be maintained during the injection process. To obtain microspheres, HAp powders were dispersed in a sodium alginate solution and spherical particles were prepared by droplet extrusion under a co-axial air stream, coupled with ionotropic gelation in the presence of Ca                        2+. This was followed by a sintering process at various temperatures and times. The morphology of microspheres was observed under SEM, diameter measurements were performed in an optical microscope and the compression strength was evaluated using a texture analyzer. Finally, microspheres prepared using lower granulometry HAp powders and sintered at 1200{\textdegree}C for 1 hour presented the best properties and were selected as the most suitable for the envisaged application.
},
	keywords = {Air streams, Apatite, Calcium, Ceramic microspheres, Co-axial, Coagulation, Compression strengths, Diameter measurements, Gelation, Granulometry, hydroxyapatite, Injectable, Injection process, Ionotropic gelations, Mechanical properties, Microspheres, Morphological structures, Morphology and mechanical properties, Optical microscopes, Powders, SEM, Sintering, Sintering conditions, Sintering process, Sodium, Sodium alginates, Spherical particles, Starting powders, Texture analyzers},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849147162\&partnerID=40\&md5=5bf4364653a3ad10b5ab28c1a01568ab},
	author = {Oliveira, S.M. and Barrias, C.C. and Ribeiro, C.C. and Almeida, I.F. and Bahia, M.F. and Barbosa, M.A.}
}
@article {2336,
	title = {Injectability of a bone filler system based on hydroxyapatite microspheres and a vehicle with in situ gel-forming ability},
	journal = {Journal of Biomedical Materials Research - Part B Applied BiomaterialsJ. Biomed. Mater. Res. Part B Appl. Biomater.},
	volume = {87},
	year = {2008},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: JBMRGdoi: 10.1002/jbm.b.31066PubMed ID: 18437700Language of Original Document: EnglishCorrespondence Address: Oliveira, S. M.; Escola Superior de Tecnologia de Viseu (ESTV), Dep. de Eng. Mec{\^a}nica e Gest{\~a}o Industrial, 3504-510 Viseu, Portugal; email: serafim@ineb.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium carbonate, 13397-26-7, 13701-58-1, 14791-73-2, 471-34-1; gluconolactonase, 9012-44-6, 9012-73-1; hydroxyapatite, 1306-06-5, 51198-94-8; Bone Substitutes; Durapatite, 1306-06-5; GelsTradenames: Captal S, Plasma BiotalManufacturers: Plasma BiotalReferences: Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373;Grabowski, G.A., Leslie, N., Wenstrup, R., Enzyme therapy for Gaucher disease: The first 5 years (1998) Blood Rev, 12, pp. 115-133; 
Melton, L.J., Melton, L.J., Kan, S.H., Frye, M.A., Wahner, H.W., O{\textquoteright}Fallon, W.M., Riggs, B.L., Epidemiology of vertebral fractures in women (1989) Am J Epidemiol, 129, pp. 1000-1011; 
Carrodeguas, R.G., Lasa, B.V., Del Barrio, J.S., injectable acrylic bone cements for vertebroplasty with improved properties (2004) J Biomed Mater Res B Appl Biomater, 68, pp. 94-104; 
Hee, H.T., Percutaneous vertebroplasty: Current concepts and local experience (2005) Neurol India, 53, pp. 475-482; 
Hide, I.G., Gangi, A., Percutaneous vertebroplasty: History, technique and current perspectives (2004) Clin Radiol, 59, pp. 461-467; 
Verlaan, J.J., Oner, F.C., Verbout, A.J., Dhert, W.J., Temperature elevation after vertebroplasty with polymethylmethacrylate in the goat spine (2003) J Biomed Mater Res B Appl Biomater, 67, pp. 581-585; 
Hardouin, P., Grados, F., Cotten, A., Cortet, B., Should percutaneous vertebroplasty be used to treat osteoporotic fractures? An update (2001) Joint Bone Spine, 68, pp. 216-221; 
Berlemann, U., Ferguson, S.J., Nolte, L.P., Heini, P.F., Adjacent vertebral failure after vertebroplasty. A biomechanical investigation (2002) J Bone Joint Surg Br, 84, pp. 748-752; 
Wilcox, R.K., The biomechanical effect of vertebroplasty on the adjacent vertebral body: A finite element study (2006) Proc Inst Mech Eng [H], 220, pp. 565-572; 
Uppin, A.A., Hirsch, J.A., Centenera, L.V., Pfiefer, B.A., Pazianos, A.G., Choi, I.S., Occurrence of new vertebral body fracture after percutaneous vertebroplasty in patients with osteoporosis (2003) Radiology, 226, pp. 119-124; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Bioceramics, 254, pp. 877-880; 
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Alsberg, E., Anderson, K.W., Albeiruti, A., Franceschi, R.T., Mooney, D.J., Cell-interactive alginate hydrogels for bone tissue engineering (2001) J Dent Res, 80, pp. 2025-2029; 
Steinert, A., Weber, M., Dimmler, A., Julius, C., Schutze, N., Noth, U., Cramer, H., Hendrich, C., Chondrogenic differentiation of mesenchymal progenitor cells encapsulated in ultrahigh-viscosity alginate (2003) J Orthop Res, 21, pp. 1090-1097; 
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Bajpai, S.K., Sharma, S., Investigation of swelling/degradation behaviour of alginate beads crosslinked with Ca2+ and Ba2+ ions (2004) React Funct Polym, 59, pp. 129-140; 
Ouwerx, C., Velings, N., Mestdagh, M.M., Axelos, M.A.V., Physico-chemical properties and rheology of alginate gel beads formed with various divalent cations (1998) Polym Gels Networks, 6, pp. 393-408; 
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Gauthier, O., Bouler, J.M., Weiss, P., Bosco, J., Aguado, E., Daculsi, G., Short-term effects of mineral particle sizes on cellular degradation activity after implantation of injectable calcium phosphate biomaterials and the consequences for bone substitution (1999) Bone, 25, pp. 71S-74S; 
Gisep, A., Curtis, R., Hanni, M., Suhm, N., Augmentation of implant purchase with bone cements: An in vitro study of injectability and dough distribution (2006) J Biomed Mater Res B Appl Biomater, 77, pp. 114-119; 
Ginebra, M.P., Rilliard, A., Fernandez, E., Elvira, C., San Roman, J., Planell, J.A., Mechanical and rheological improvement of a calcium phosphate cement by the addition of a polymeric drug (2001) J Biomed Mater Res, 57, pp. 113-118; 
Krebs, J., Ferguson, S.J., Bohner, M., Baroud, G., Steffen, T., Heini, P.F., Clinical measurements of cement injection pressure during vertebroplasty (2005) Spine, 30, pp. E118-E122; 
Bohner, M., Baroud, G., Injectability of calcium phosphate pastes (2005) Biomaterials, 26, pp. 1553-1563; 
Hernandez, L., Gurruchaga, M., Goni, I., Influence of powder particle size distribution on complex viscosity and other properties of acrylic bone cement for vertebroplasty and kyphoplasty (2006) J Biomed Mater Res B Appl Biomater, 77, pp. 98-103; 
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},
	month = {2008///},
	pages = {49 - 58},
	abstract = {The aim of this study was to test the injectability of a bone filler system based on the combination of ceramic microspheres with a gel-like vehicle, for noninvasive surgery. Porous hydroxyapatite microspheres with a uniform size and an average diameter of 535 {\textpm} 38 μm were prepared, and their compression strength and friability were tested. The sodium-alginate solution with a concentration of 7.25\% (w/v) was used as the vehicle. To promote its in situ gelation, calcium carbonate and D-gluconic-δ-lactone were added to the solution. Microspheres were mixed with the vehicle at different percentages (20-40 wt \%). Gelation times in the range of 8-20 min, were obtained, depending on the formulation. Mixtures of HAp microspheres with alginate solution at 7.25\% originating a gel in 11 min present an adequate handling time to perform an injection. Their injectability was evaluated using an injection device commonly employed in vertebroplasty surgical procedures, coupled to a texturometer in compression mode. Using an extrusion rate of 0.1 mm/s, the force required to extrude any of the mixtures tested was lower than 100 N. For an extrusion rate of 1 mm/s mixtures with 40 wt \% of microspheres were very difficult to inject. Mixtures with 35 wt \% of microspheres presented the best compromise between injectability and compression strength of the gelled system. MicroCT analysis revealed a homogeneous distribution of the microspheres inside the vehicle, as well as full interconnection of the intra-microspheres spaces. The compression strength for the gelled systems ranged from 80 kPa (gel itself) to 600 kPa (composite with 40 Wt \% of microspheres). {\textcopyright} 2008 Wiley Periodicals, Inc.},
	keywords = {Alginate, alginic acid, Apatite, article, Average diameter, bioengineering, Bone, bone filler system, Bone Substitutes, Calcium, Calcium carbonate, Ceramic, Ceramic microspheres, Coagulation, Colloids, compression, Compression mode, Compression strengths, Compressive Strength, Computerized tomography, Concentration (process), Data compression, Durapatite, Extrusion, Extrusion rates, Fillers, Forming, Gel-forming ability, Gelation, Gels, gluconolactonase, Handling time, Homogeneous distribution, hydrogel, hydroxyapatite, In situ gelation, in situ hybridization, In-situ, Injectability, injection, Injection (oil wells), Micro-CT, microsphere, Microspheres, Mixtures, non invasive procedure, Particle Size, Porous hydroxyapatite, Sodium, Surgery, Surgical procedures, Uniform size, Vehicles, Vertebroplasty},
	isbn = {15524973 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-52449123139\&partnerID=40\&md5=cc648bad01cb2c6aa01d885acac10e8a},
	author = {Oliveira, S.M. and Barrias, C.C. and Almeida, I.F. and Costa, P.C. and Pena Ferreira, M.R. and Bahia, M.F. and Barbosa, M.A.}
}
