



@article {2245,
	title = {Innovative macroporous granules of nanostructured-hydroxyapatite agglomerates: Bioactivity and osteoblast-like cell behaviour},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32916PubMed ID: 20845490Language of Original Document: EnglishCorrespondence Address: Laranjeira, M. S.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: dce06004@fe.up.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; colony stimulating factor 1, 81627-83-0; hydroxyapatite, 1306-06-5, 51198-94-8; osteoprotegerin, 205944-50-9; polyurethan, 61789-63-7; Biocompatible Materials; Biological Markers; Durapatite, 1306-06-5; PolyurethanesReferences: Ferraz, M., Monteiro, F., Manuel, C., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80;Murugan, R., Ramakrishna, S., Development of nanocomposites for bone grafting (2005) Compos Sci Technol, 65, pp. 2385-2406; 
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},
	month = {2010///},
	pages = {891 - 900},
	abstract = {To modulate the biological response of implantable granules, two types of bioactive porous granules composed of nanostructured-hydroxyapatite (HA) agglomerates and microstructured-HA, respectively, were prepared using a polyurethane sponge impregnation and burnout method. The resulting granules presented a highly porous structure with interconnected porosity. Both types of granules were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and mercury intrusion porosimetry. Results showed that nanostructed-HA granules presented higher surface area and porosity than microstructured-HA granules. In vitro testing using MG63 human osteoblast-like cells showed that on both types of surfaces cells were able to adhere, proliferate, and migrate through the macropores, and a higher growth rate was achieved on nanostructured-HA granules than on microstructured-HA granules (76 and 40\%, respectively). In addition, these cells maintained similar expression levels of osteoblastic-associated markers namely collagen type I, alkaline phosphatase, bone morphogenetic protein-2, macrophage colony-stimulating factor, and osteoprotegerin. These innovative nanostructured-HA granules may be considered as promising bioceramic alternative matrixes for bone regeneration and drug release application. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {Agglomeration, alkaline phosphatase, Apatite, article, Bioceramics, Biocompatible Materials, Biological Markers, Biological materials, Biological response, Biomaterials, Bone, bone morphogenetic protein 2, Bone morphogenetic protein-2, Bone Regeneration, cell adhesion, cell function, cell granule, cell migration, cell proliferation, Cells, Cultured, collagen type 1, Collagen type I, colony stimulating factor 1, Colony-stimulating factor, drug activity, Drug release, Durapatite, Expression levels, Fourier transform infrared spectroscopy, Fourier transforms, Granulation, Granules, growth rate, human, human cell, Human osteoblast-like cells, Humans, hydroxyapatite, In-vitro, infrared spectroscopy, Interconnected porosity, Macro-porosity, Macropores, Macroporosity, Macroporous, materials testing, matrix, Mercury (metal), Mercury intrusion porosimetry, Microscopy, Electron, Scanning, Microstructured, Nano-materials, Nano-structured, nanomaterial, Nanomaterials, Nanostructured materials, Nanostructures, osteoblast, Osteoblast response, Osteoblast-like cells, Osteoblasts, Osteoprotegerin, Phosphatases, polyurethan, Polyurethane sponge, Polyurethanes, porosity, Porous structures, Reverse Transcriptase Polymerase Chain Reaction, Scanning electron microscopy, SEM, Spectroscopy, Fourier Transform Infrared, Surface area, surface property, X ray diffraction, X-Ray Diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78249252998\&partnerID=40\&md5=09d51d6c49d9f10e6c2d479b1fe4ace0},
	author = {Laranjeira, M.S. and Fernandes, M.H. and Monteiro, F.J.}
}
