



@article {3298,
	title = {Effect of cell density on mesenchymal stem cells aggregation in RGD-alginate 3D matrices under osteoinductive conditions},
	journal = {Macromolecular Bioscience},
	volume = {14},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {759 - 771},
	abstract = {Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered "normal" activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell-cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering. {\textcopyright} 2014 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {alginate hydrogels, encapsulation, Self-assembly, Tissue engineering},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84902536382\&partnerID=40\&md5=d6659c40247c561753aca566f938d788},
	author = {Maia, F.R. and Louren{\c c}o, A.H. and Granja, P.L. and Gon{\c c}alves, R.M. and Barrias, C.C.}
}
@article {3311,
	title = {The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {8144 - 8153},
	abstract = {Intervertebral disc (IVD) degeneration is the leading cause of low back pain and disability in the active population. Transplantation of mesenchymal stem cells (MSCs) in a hydrogel carrier can induce regenerative effects in degenerated IVDs. Moreover, it was found that degenerative discs release chemoattractants effective in MSC recruitment. Based on these findings, we hypothesized that an injectable hydrogel that can enhance the number of migrated MSCs in the IVD and provide a suitable matrix for their survival and differentiation would be ideal. The purpose of this study was to evaluate the potential of a thermoreversible hyaluronan-poly(N-isopropylacrylamide) (HAP) hydrogel as chemoattractant delivery system to recruit human MSCs in degenerative IVDs. The results demonstrate that HAP hydrogels containing stromal cell derived factor-1 (SDF-1) significantly increased the number of MSCs migrating into nucleotomized discs compared with discs treated with only HAP or SDF-1 in solution. HAP hydrogels releasing SDF-1 enhanced both the number of recruited cells and their migration distance in the IVD tissue. Furthermore, this phenomenon was dependent on MSC donor age. In conclusion, HAP SDF-1 is effective for the recruitment of stem cells in the IVD, thus opening new possibilities for the development of regenerative therapies based on endogenous cell migration. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {Intervertebral disc, Mesenchymal Stem Cells, Recruitment, Stromal cell derived factor-1, Thermoreversible hyaluronan hydrogel},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903746142\&partnerID=40\&md5=1366ab2e3399d0a363aab2e356bbdf96},
	author = {Pereira, C.L. and Gon{\c c}alves, R.M. and Peroglio, M. and Pattappa, G. and D{\textquoteright}Este, M. and Eglin, D. and Barbosa, M.A. and Alini, M. and Grad, S.}
}
@article {2759,
	title = {Adsorbed fibrinogen leads to improved bone regeneration and correlates with differences in the systemic immune response},
	journal = {Acta Biomaterialia},
	volume = {9},
	year = {2013},
	note = {Export Date: 23 May 2013Source: ScopusArticle in Press},
	month = {07/2013},
	pages = {7209-7217},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84876834487\&partnerID=40\&md5=2d00f0bc55f461c9c979548276f0e53d},
	author = {Santos, S.G. and Lamghari, M. and Almeida, C.R. and Oliveira, M.I. and Neves, N. and Ribeiro, A.C. and Barbosa, J.N. and Barros, R. and Maciel, J. and Martins, M.C.L. and Gon{\c c}alves, R.M. and Barbosa, M.A.}
}
@article {2203,
	title = {Biosynthesis of highly pure poly-γ-glutamic acid for biomedical applications},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {23},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-012-4639-xPubMed ID: 22532096Language of Original Document: EnglishCorrespondence Address: Pereira, C.L.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: Catarina.pereira@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; polyglutamic acid, 24991-23-9, 25513-46-6; Biocompatible Materials; Polyglutamic Acid, 25513-46-6; poly(gamma-glutamic acid)References: Ratner, B.D., Hoffman, A.S., Shoen, F.J., Lemons, J.E., (2004) Natural Materials. Biomaterials Science-an Introduction to Materials in Medicine, , 2nd ed. San Diego, CA: Academic Press;Buescher, J.M., Margaritis, A., Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries (2007) Critical Reviews in Biotechnology, 27 (1), pp. 1-19. , DOI 10.1080/07388550601166458, PII 773236840; 
Kang, H.-S., Park, S.-H., Lee, Y.-G., Son, T.-I., Polyelectrolyte complex hydrogel composed of chitosan and poly(γ-glutamic acid) for biological application: Preparation, physical properties, and cytocompatibility (2007) Journal of Applied Polymer Science, 103 (1), pp. 386-394. , DOI 10.1002/app.24623; 
Lin, Y.-H., Chung, C.-K., Chen, C.-T., Liang, H.-F., Chen, S.-C., Sung, H.-W., Preparation of nanoparticles composed of chitosan/poly-γ-glutamic acid and evaluation of their permeability through Caco-2 cells (2005) Biomacromolecules, 6 (2), pp. 1104-1112. , DOI 10.1021/bm049312a; 
Sanda, F., Fujiyama, T., Endo, T., Chemical synthesis of polygamma-glutamic acid by polycondensation of gamma-glutamic acid dimer: Synthesis and reaction of poly gamma-glutamic acid methyl ester (2000) J Polym Sci, Part A: Polym Chem, 39, pp. 732-741; 
Ashiuchi, M., Shimanouchi, K., Nakamura, H., Kamei, T., Soda, K., Park, C., Sung, M.-H., Misono, H., Enzymatic synthesis of high-molecular-mass poly-γ-glutamate and regulation of its stereochemistry (2004) Applied and Environmental Microbiology, 70 (7), pp. 4249-4255. , DOI 10.1128/AEM.70.7.4249-4255.2004; 
Shih, I.-L., Van, Y.-T., The production of poly-(γ-glutamic acid) from microorganisms and its various applications (2001) Bioresource Technology, 79 (3), pp. 207-225. , DOI 10.1016/S0960-8524(01)00074-8, PII S0960852401000748; 
Prodhomme, E.J.F., Tutt, A.L., Glennie, M.J., Bugg, T.D.H., Multivalent Conjugates of Poly-γ-D-glutamic Acid from Bacillus licheniformis with Antibody F(ab{\textquoteright}) and Glycopeptide Ligands (2003) Bioconjugate Chemistry, 14 (6), pp. 1148-1155. , DOI 10.1021/bc020019m; 
Schneerson, R., Kubler-Kielb, J., Liu, T.-Y., Dai, Z.-D., Leppla, S.H., Yergey, A., Backlund, P., Robbins, J.B., Poly(γ-D-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: A potential addition to the anthrax vaccine (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8945-8950. , DOI 10.1073/pnas.1633512100; 
Edde, B., Rossier, J., Le Caer, J., Desbruyeres, E., Gros, F., Denoulet, P., Posttranslational glutamylation of alpha-tubulin (1990) Science, 247 (4938), pp. 83-85; 
Sung, M.-H., Park, C., Kim, C.-J., Poo, H., Soda, K., Ashiuchi, M., Natural and edible biopolymer poly-γ-glutamic acid: Synthesis, production, and applications (2005) Chemical Record, 5 (6), pp. 352-366. , DOI 10.1002/tcr.20061; 
Kunioka, M., Biosynthesis and chemical reactions of poly(amino acid)s from microorganisms (1997) Applied Microbiology and Biotechnology, 47 (5), pp. 469-475. , DOI 10.1007/s002530050958; 
Ashiuchi, M., Soda, K., Misono, H., A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336: Gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells (1999) Biochemical and Biophysical Research Communications, 263 (1), pp. 6-12. , DOI 10.1006/bbrc.1999.1298; 
Park, C., Choi, J.-C., Choi, Y.-H., Nakamura, H., Shimanouchi, K., Horiuchi, T., Misono, H., Sung, M.-H., Synthesis of super-high-molecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. chungkookjang (2005) Journal of Molecular Catalysis B: Enzymatic, 35 (4-6), pp. 128-133. , DOI 10.1016/j.molcatb.2005.06.007; 
Goto, A., Kunioka, M., Biosynthesis and hydrolysis of poly (c-glutamic acid) from Bacillus subtilis IFO3335 (1992) Biosci Biotechnol Biochem, 56 (7), pp. 1031-1035; 
Kubota, H., Matsunobu, T., Uotani, K., Takebe, H., Satoh, A., Tanaka, T., Production of poly(c-glutamic acid) by Bacillus subtilis F-2-01 (1993) Biosci Biotechnol Biochem, 57 (7), pp. 1212-1213; 
Kunioka, M., Goto, A., Biosynthesis of poly(γ-glutamic acid) from L-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335 (1994) Applied Microbiology and Biotechnology, 40 (6), pp. 867-872. , DOI 10.1007/s002530050082; 
Ashiuchi, M., Misono, H., Biochemistry and molecular genetics of poly-γ-glutamate synthesis (2002) Applied Microbiology and Biotechnology, 59 (1), pp. 9-14. , DOI 10.1007/s00253-002-0984-x; 
Hsieh, C.-Y., Tsai, S.-P., Wang, D.-M., Chang, Y.-N., Hsieh, H.-J., Preparation of γ-PGA/chitosan composite tissue engineering matrices (2005) Biomaterials, 26 (28), pp. 5617-5623. , DOI 10.1016/j.biomaterials.2005.02.012, PII S0142961205001614; 
Richard, A., Margaritis, A., Rheology, oxygen transfer, and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis (2003) Biotechnology and Bioengineering, 82 (3), pp. 299-305. , DOI 10.1002/bit.10568; 
Peng, S.-F., Yang, M.-J., Su, C.-J., Chen, H.-J., Lee, P.-W., Wei, M.-C., Effects of incorporation of poly(gamma-glutamic acid) in chitosan/DNA complex nanoparticles on cellular uptake and transfection efficiency (2009) Biomaterials, 30, pp. 1797-1808; 
Lin, Y.-H., Mi, F.-L., Chen, C.-T., Chang, W.-C., Peng, S.-F., Liang, H.-F., Sung, H.-W., Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery (2007) Biomacromolecules, 8 (1), pp. 146-152. , DOI 10.1021/bm0607776; 
Lee, P.-W., Peng, S.-F., Su, C.-J., Mi, F.-L., Chen, H.-L., Wei, M.-C., Lin, H.-J., Sung, H.-W., The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery (2008) Biomaterials, 29 (6), pp. 742-751. , DOI 10.1016/j.biomaterials.2007.10.034, PII S014296120700837X; 
Hajdu, I., Bodn{\'a}r, M., Filipcsei, G., Hartmann, J., Dar{\'o}czi, L., Zr{\'\i}nyi, M., Nanoparticles prepared by self-assembly of Chitosan and poly-γ-glutamic acid (2008) Colloid Polym Sci, 286 (3), pp. 343-350; 
Keresztessy, Z., Bodn{\'a}r, M., Ber, E., Hajdu, I., Zhang, M., Hartmann, J., Self-assembling chitosan/poly-γ-glutamic acid nanoparticles for targeted drug delivery (2009) Colloid Polym Sci, 287 (7), pp. 759-765; 
Nelson, D., Cox, M., Amino acids peptides and proteins (2005) Lehnninger Principles of Biochemistry, pp. 78-79. , Freeman WH editor. New York: WH Freemand and Company; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57 (1), pp. 35-52. , DOI 10.1016/S0939-6411(03)00160-7; 
Yamaguchi, F., Ogawa, Y., Kikuchi, M., Yuasa, K., Motai, H., Detection of γ-Polyglutamic acid (γ-PGA) by SDS-PAGE (1996) Biosci Biotech Biochem, 60 (2), pp. 255-258; 
Antunes, J.C., Pereira, C.L., Molinos, M., Ferreira-Da-Silva, F., Gon{\c c}alves, R.M., Barbosa, M.A., Layer-by-layer self-assembly of polyelectrolyte complexes of Chitosam and poly(gamma-glutamic acid) (2011) Biomacromolecules, 12 (12), pp. 4183-4195; 
Georgiou, C., Grintzalis, K., Zervoudakis, G., Papapostolou, I., Mechanism of Coomassie brilliant blue G-250 binding to proteins: A hydrophobic assay for nanogram quantities of proteins (2008) Anal Bioanal Chem, 391 (1), pp. 391-403; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies - Table and Charts, , England: Jonh Wiley \&Sons Ltd; 
Birrer, G.A., Cromwick, A.M., Gross, R.A., Gamma-poly(glutamic acid) formation by Bacillus licheniformis 9945a: Physiological and biochemical studies (1994) Int J Biol Macromol, 16 (5), pp. 265-275; 
Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L., Alonso, M.J., Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers (1997) Journal of Applied Polymer Science, 63 (1), pp. 125-132
},
	month = {2012///},
	pages = {1583 - 1591},
	abstract = {The remarkable properties of poly-aminoacids, mainly their biocompatibility and biodegradability, have prompted an increasing interest in these polymers for biomedical applications. Poly-γ-glutamic acid (γ-PGA) is one of the most interesting poly-aminoacids with potential applications as a biomaterial. Here we describe the production and characterization of γ-PGA by Bacillus subtilis natto. The γ-PGA was produced with low molecular weight (10-50 kDa), high purity grade (>99 \%) and a D-/L-glutamate ratio of 50-60/50-40 \%. To evaluate the feasibility of using this γ-PGA as a biomaterial, chitosan (Ch)/γ-PGA nanoparticles were prepared by the coacervation method at pH ranging from 3.0 to 5.0, with dimensions in the interval 214-221 nm with a poly-dispersion index of ca. 0.2. The high purity of γ-PGA produced by this method, which is firstly described here, renders this biopolymer suitable for biomedical applications. Moreover, the Ch/γ-PGA nanocomplexes developed in this investigation can be combined with biologically active substances for their delivery in the organism. The fact that the assembly between Ch and γ-PGA relies on electrostatic interactions enables addition of other molecules that can be released into the medium through changes from acidic to physiological pH, without loss in biological activity. {\textcopyright} Springer Science+Business Media, LLC 2012.},
	keywords = {Amino acids, animal cell, article, Bacillus subtilis, Bacillus subtilis natto, Bioactivity, Biocompatibility, Biocompatible Materials, biodegradability, Biodegradable polymers, Biodegradation, Biologically active substances, biomaterial, Biomedical applications, biomedicine, biosynthesis, chitosan, circular dichroism, complex formation, controlled study, dispersion, Electrophoresis, Polyacrylamide Gel, feasibility study, Functional polymers, gamma polyglutamic acid, Glutamic acid, High purity, Low molecular weight, Medical applications, molecular interaction, molecular weight, Nanocomplexes, nanoparticle, nonhuman, pH, Physiological pH, polyglutamic acid, Potential applications, priority journal, purification, Spectroscopy, Fourier Transform Infrared, static electricity, Stereoisomerism, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84863314791\&partnerID=40\&md5=d0d618bb2684c8e80e984cfe35f3eb77},
	author = {Pereira, C.L. and Antunes, J.C. and Gon{\c c}alves, R.M. and Ferreira-da-Silva, F. and Barbosa, M.A.}
}
@article {2211,
	title = {Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials},
	journal = {Journal of the Royal Society InterfaceJ. R. Soc. Interface},
	volume = {9},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1098/rsif.2011.0357Language of Original Document: EnglishCorrespondence Address: Almeida, C.R.; INEB-Instituto de Engenharia Biom{\'e}dica, Biomaterials Division, NEWTherapies Group, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cralmeid@ineb.up.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; chitosan, 9012-76-4; fibrinogen, 9001-32-5; gamma interferon, 82115-62-6References: Mountziaris, P.M., Mikos, A.G., Modulation of the inflammatory response for enhanced bone tissue regeneration (2008) Tissue Eng. Part B Rev., 14, pp. 179-186. , doi:10. 1089/ten.teb.2008.0038;Anderson, J.M., Rodriguez, A., Chang, D.T., Foreign body reaction to biomaterials (2008) Semin. Immunol., 20, pp. 86-100. , doi:10.1016/j.smim.2007.11.004; 
Agaiby, A.D., Dyson, M., Immuno-inflammatory cell dynamics during cutaneous wound healing (1999) J. Anat., 195, pp. 531-542. , doi:10.1046/j.1469-7580.1999.19540531.x; 
Vivier, E., Tomasello, E., Baratin, M., Walzer, T., Ugolini, S., Functions of natural killer cells (2008) Nat. Immunol., 9, pp. 503-510. , doi:10.1038/ni1582; 
Aggarwal, S., Pittenger, M.F., Human mesenchymal stem cells modulate allogeneic immune cell responses (2005) Blood, 105, pp. 1815-1822. , doi:10.1182/blood- 2004-04-1559; 
Caplan, A.I., Adult mesenchymal stem cells for tissue engineering versus regenerative medicine (2007) J. Cell. Physiol., 213, pp. 341-347. , doi:10.1002/jcp.21200; 
Pittenger, M.F., Multilineage potential of adult human mesenchymal stem cells (1999) Science, 284, pp. 143-147. , doi:10.1126/science.284.5411.143; 
Poggi, A., Prevosto, C., Massaro, A.M., Negrini, S., Urbani, S., Pierri, I., Saccardi, R., Zocchi, M.R., Interaction between human NK cells and bone marrow stromal cells induces NK cell triggering: Role of NKp30 and NKG2D receptors (2005) J. Immunol., 175, pp. 6352-6360. , doi:10.175/10/6352[pii]; 
Rasmusson, I., Ringden, O., Sundberg, B., Le Blanc, K., Mesenchymal stem cells inhibit the formation of cytotoxic T lymphocytes, but not activated cytotoxic T lymphocytes or natural killer cells (2003) Transplantation, 76, pp. 1208-1213. , doi:10.1097/01.TP.0000082540.43730.80; 
G{\"o}therstr{\"o}m, C., Lundqvist, A., Duprez, I.R., Childs, R., Berg, L., Le Blanc, K., Fetal and adult multipotent mesenchymal stromal cells are killed by different pathways (2010) Cytotherapy, 13, pp. 269-278. , doi:10.3109/14653249.2010.523077; 
Jewett, A., Arasteh, A., Tseng, H.C., Behel, A., Arasteh, H., Yang, W., Cacalano, N.A., Paranjpe, A., Strategies to rescue mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) from NK cell mediated cytotoxicity (2010) PLoS ONE, 5, pp. e9874. , doi:10.1371/journal.pone. 0009874; 
Sotiropoulou, P.A., Perez, S.A., Gritzapis, A.D., Baxevanis, C.N., Papamichail, M., Interactions between human mesenchymal stem cells and natural killer cells (2006) Stem Cells, 24, pp. 74-85. , doi:10.1634/stemcells.2004-0359; 
Spaggiari, G.M., Capobianco, A., Becchetti, S., Mingari, M.C., Moretta, L., Mesenchymal stem cell-natural killer cell interactions: Evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2- Induced NK-cell proliferation (2006) Blood, 107, pp. 1484-1490. , doi:10.1182/blood-2005-07-2775; 
Tseng, H.C., Increased lysis of stem cells but not their differentiated cells by natural killer cells; dedifferentiation or reprogramming activates NK cells (2010) PLoS ONE, 5, pp. e11590. , doi:10.1371/journal.pone.0011590; 
Krampera, M., Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells (2006) Stem Cells, 24, pp. 386-398. , doi:10.1634/stemcells.2005-0008; 
Pradier, A., Passweg, J., Villard, J., Kindler, V., Human bone marrow stromal cells and skin fibroblasts inhibit natural killer cell proliferation and cytotoxic activity (2010) Cell Transplant., , doi:10.3727/096368910X 536545; 
Selmani, Z., Human leukocyte antigen-G5 secretion by human mesenchymal stem cells is required to suppress T lymphocyte and natural killer function and to induce CD4+CD25highFOXP3+ regulatory T cells (2008) Stem Cells, 26, pp. 212-222. , doi:10.1634/stemcells. 2007-0554; 
Spaggiari, G.M., Capobianco, A., Abdelrazik, H., Becchetti, F., Mingari, M.C., Moretta, L., Mesenchymal stem cells inhibit natural killer-cell proliferation, cytotoxicity, and cytokine production: Role of indoleamine 2,3-dioxygenase and prostaglandin E2 (2008) Blood, 111, pp. 1327-1333. , doi:10.1182/blood-2007-02- 074997; 
Salem, H.K., Thiemermann, C., Mesenchymal stromal cells: Current understanding and clinical status (2010) Stem Cells, 28, pp. 585-596. , doi:10.1002/stem.269; 
Dimitriou, R., Tsiridis, E., Giannoudis, P.V., Current concepts of molecular aspects of bone healing (2005) Injury, 36, pp. 1392-1404. , doi:10.1016/j.injury.2005.07.019; 
Rifas, L., T-cell cytokine induction of BMP-2 regulates human mesenchymal stromal cell differentiation and mineralization (2006) J. Cell. Biochem., 98, pp. 706-714. , doi:10.1002/jcb.20933; 
Rifas, L., Arackal, S., Weitzmann, M.N., Inflammatory T cells rapidly induce differentiation of human bone marrow stromal cells into mature osteoblasts (2003) J. Cell. Biochem., 88, pp. 650-659. , doi:10.1002/jcb.10436; 
Champagne, C.M., Takebe, J., Offenbacher, S., Cooper, L.F., Macrophage cell lines produce osteoinductive signals that include bone morphogenetic protein-2 (2002) Bone, 30, pp. 26-31. , doi:10.1016/S8756-3282(01)00638-X; 
Gerstenfeld, L.C., Cho, T.J., Kon, T., Aizawa, T., Cruceta, J., Graves, B.D., Einhorn, T.A., Impaired intramembranous bone formation during bone repair in the absence of tumor necrosis factor-alpha signaling (2001) Cells Tissues Organs, 169, pp. 285-294. , doi:10.1159/ 000047893; 
Gerstenfeld, L.C., Cho, T.J., Kon, T., Aizawa, T., Tsay, A., Fitch, J., Barnes, G.L., Einhorn, T.A., Impaired fracture healing in the absence of TNF-alpha signaling: The role of TNF-α in endochondral cartilage resorption (2003) J. Bone Miner. Res., 18, pp. 1584-1592. , doi:10.1359/jbmr.2003.18.9.1584; 
Duque, G., Huang, D.C., Macoritto, M., Rivas, D., Yang, X.F., Ste-Marie, L.G., Kremer, R., Autocrine regulation of interferon gamma in mesenchymal stem cells plays a role in early osteoblastogenesis (2009) Stem Cells, 27, pp. 550-558. , doi:10.1634/stemcells.2008-0886; 
Honczarenko, M., Le, Y., Swierkowski, M., Ghiran, I., Glodek, A.M., Silberstein, L.E., Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors (2006) Stem Cells, 24, pp. 1030-1041. , doi:10.1634/stemcells.2005-0319; 
Robertson, M.J., Role of chemokines in the biology of natural killer cells (2002) J. Leukoc Biol., 71, pp. 173-183; 
Rodrigues, S.N., Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367. , doi:10.1016/j.biomaterials.2006.06.010; 
Peled, E., Boss, J., Bejar, J., Zinman, C., Seliktar, D., A novel poly(ethylene glycol)-fibrinogen hydrogel for tibial segmental defect repair in a rat model (2007) J. Biomed. Mater. Res., 80, pp. 874-884. , doi:10.1002/jbm. a.30928; 
Bryceson, Y.T., March, M.E., Ljunggren, H.G., Long, E.O., Activation, coactivation, and costimulation of resting human natural killer cells (2006) Immunol. Rev., 214, pp. 73-91. , doi:10.1111/j.1600-065X.2006.00457.x; 
Muto, S., Vetvicka, V., Ross, G.D., CR3 (CD11b/ CD18) expressed by cytotoxic T cells and natural killer cells is upregulated in a manner similar to neutrophil CR3 following stimulation with various activating agents (1993) J. Clin. Immunol., 13, pp. 175-184. , doi:10.1007/ BF00919970; 
Ross, G.D., Vetvicka, V., CR3 (CD11b, CD18): A phagocyte and NK cell membrane receptor with multiple ligand specificities and functions (1993) Clin. Exp. Immunol., 92, pp. 181-184. , doi:10.1111/j.1365-2249.1993.tb03377.x; 
Lishko, V.K., Kudryk, B., Yakubenko, V.P., Yee, V.C., Ugarova, T.P., Regulated unmasking of the cryptic binding site for integrin alpha M beta 2 in the gamma C-domain of fibrinogen (2002) Biochemistry, 41, pp. 12942-12951. , doi:10.1021/bi026324c; 
Mwale, F., Iordanova, M., Demers, C.N., Steffen, T., Roughley, P., Antoniou, J., Biological evaluation of chitosan salts cross-linked to genipin as a cell scaffold for disk tissue engineering (2005) Tissue Eng., 11, pp. 130-140. , doi:10.1089/ten.2005.11.130; 
Prabaharan, M., Jayakumar, R., Chitosan-graft-beta-cyclodextrin scaffolds with controlled drug release capability for tissue engineering applications (2009) Int. J. Biol. Macromol., 44, pp. 320-325. , doi:10.1016/j.ijbiomac.2009.01.005; 
Agnihotri, S.A., Mallikarjuna, N.N., Aminabhavi, T.M., Recent advances on chitosan-based micro-and nanoparticles in drug delivery (2004) J. Control Release, 100, pp. 5-28. , doi:10.1016/j.jconrel.2004.08.010; 
Barbosa, M.A., Granja, P.L., Barrias, C.C., Amaral, I.F., Polysaccharides as scaffolds for bone regeneration (2005) ITBM-RBM, 26, pp. 212-217. , doi:10.1016/j.rbmret.2005.04.006; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) Eur. J. Pharm. Biopharm., 57, pp. 35-52. , doi:10.1016/S0939-6411(03)00160-7; 
Amaral, I.F., Cordeiro, A.L., Sampaio, P., Barbosa, M.A., Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation (2007) J. Biomater. Sci. Polym. Ed., 18, pp. 469-485. , doi:10.1163/ 156856207780425068; 
Barbosa, J.N., Amaral, I.F., Aguas, A.P., Barbosa, M.A., Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds (2010) J. Biomed. Mater. Res., 93 A, pp. 20-28. , doi:10.1002/jbm.a.32499; 
Oliveira, S.M., Mijares, D.Q., Turner, G., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., Engineering endochondral bone: In vivo studies (2009) Tissue Eng. Part A, 15, pp. 635-643. , doi:10.1089/ten.tea.2008.0052; 
Amaral, I.F., Sampaio, P., Barbosa, M.A., Three-dimensional culture of human osteoblastic cells in chitosan sponges: The effect of the degree of acetylation (2006) J. Biomed. Mater. Res., 76, pp. 335-346. , doi:10.1002/jbm.a.30522; 
Goncalves, R.M., Martins, M.C., Almeida-Porada, G., Barbosa, M.A., Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers (2009) Biomaterials, 30, pp. 6879-6887. , doi:10.1016/j.biomaterials.2009.09.010; 
Dominici, M., Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement (2006) Cytotherapy, 8, pp. 315-317. , doi:10.1080/14653240600855905; 
Siddappa, R., Licht, R., Van Blitterswijk, C., De Boer, J., Donor variation and loss of multipotency during in vitro expansion of human mesenchymal stem cells for bone tissue engineering (2007) J. Orthop. Res., 25, pp. 1029-1041. , doi:10.1002/jor.20402; 
Maeda, Y., Kimura, Y., Antitumor Effects of Various Low-Molecular-Weight Chitosans Are Due to Increased Natural Killer Activity of Intestinal Intraepithelial Lymphocytes in Sarcoma 180-Bearing Mice (2004) Journal of Nutrition, 134 (4), pp. 945-950; 
Zaharoff, D.A., Rogers, C.J., Hance, K.W., Schlom, J., Greiner, J.W., Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination (2007) Vaccine, 25, pp. 2085-2094. , doi:10. 1016/j.vaccine.2006.11.034; 
Tang, L., Eaton, J.W., Fibrin(ogen) mediates acute inflammatory responses to biomaterials (1993) J. Exp. Med., 178, pp. 2147-2156. , doi:10.1084/jem.178.6.2147; 
Drew, A.F., Liu, H., Davidson, J.M., Daugherty, C.C., Degen, J.L., Wound-healing defects in mice lacking fibrinogen (2001) Blood, 97, pp. 3691-3698. , doi:10.1182/blood. V97.12.3691; 
Flick, M.J., Du, X., Degen, J.L., Fibrin(ogen)- alpha M beta 2 interactions regulate leukocyte function and innate immunity in vivo (2004) Exp. Biol. Med. (Maywood), 229, pp. 1105-1110. , doi:10.229/11/1105[pii]; 
Culley, F.J., Natural killer cell signal integration balances synapse symmetry and migration (2009) PLoS Biol., 7, pp. e1000159. , doi:10.1371/journal.pbio.1000159; 
Dustin, M.L., Cell adhesion molecules and actin cytoskeleton at immune synapses and kinapses (2007) Curr. Opin. Cell Biol., 19, pp. 529-533. , doi:10.1016/j.ceb.2007.08.003; 
Jacobs, R., Hintzen, G., Kemper, A., Beul, K., Kempf, S., Behrens, G., Sykora, K.W., Schmidt, R.E., CD56bright cells differ in their KIR repertoire and cytotoxic features from CD56dim NK cells (2001) Eur. J. Immunol., 31, pp. 3121-3127. , doi:10.1002/1521-4141(2001010)31: 10〈3121::AID-IMMU3121〉3.0. CO;2-4; 
Anfossi, N., Human NK cell education by inhibitory receptors for MHC class I (2006) Immunity, 25, pp. 331-342. , doi:10.1016/j.immuni.2006.06.013; 
Nolte-{\textquoteright}t Hoen, E.N.M., Almeida, C.R., Cohen, N.R., Nedvetzki, S., Yarwood, H., Davis, D.M., Increased surveillance of cells in mitosis by human NK cells suggests a novel strategy for limiting tumor growth and viral replication (2006) Blood, 109, pp. 670-673. , doi:10.1182/blood-2006-07- 036509; 
Hemeda, H., Jakob, M., Ludwig, A.K., Giebel, B., Lang, S., Brandau, S., Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells (2010) Stem Cells Dev., 19, pp. 693-706; 
Liippo, J., Toriseva, M., K{\"a}h{\"a}ri, V.-M., Natural killer cell in wound healing (2009) Natural Killer Cells - Basic Science and Clinical Application, pp. 519-525. , eds M. Lotze \& A. Thomson, New York, NY: Academic Press; 
Soderstrom, K., Stein, E., Colmenero, P., Purath, U., Muller-Ladner, U., De Matos, C.T., Tarner, I.H., Engleman, E.G., Natural killer cells trigger osteoclastogenesis and bone destruction in arthritis (2010) Proc. Natl Acad. Sci. USA, 107, pp. 13028-13033. , doi:10.1073/pnas.1000546107
},
	month = {2012///},
	pages = {261 - 271},
	abstract = {An exacerbated inflammatory response questions biomaterial biocompatibility, but on the other hand, inflammation has a central role in the regulation of tissue regeneration. Therefore, it may be argued that an {\textquoteright}ideal{\textquoteright} inflammatory response is crucial to achieve efficient tissue repair/regeneration. Natural killer (NK) cells, being one of the first populations arriving at an injury site, can have an important role in regulating bone repair/regeneration, particularly through interactions with mesenchymal stem/stromal cells (MSCs). Here, we studied how biomaterials designed to incorporate inflammatory signals affected NK cell behaviour and NK cell-MSC interactions. Adsorption of the pro-inflammatory molecule fibrinogen (Fg) to chitosan films led to a 1.5-fold increase in adhesion of peripheral blood human NK cells, without an increase in cytokine secretion. Most importantly, it was found that NK cells are capable of stimulating a threefold increase in human bone marrow MSC invasion, a key event taking place in tissue repair, but did not affect the expression of the differentiation marker alkaline phosphatase (ALP). Of significant importance, this NK cell-mediated MSC recruitment was modulated by Fg adsorption. Designing novel biomaterials leading to rational modulation of the inflammatory response is proposed as an alternative to current bone regeneration strategies. {\textcopyright} 2011 The Royal Society.},
	keywords = {Adhesion, Adsorption, alkaline phosphatase, article, Biocompatibility, biomaterial, Biomaterials, blood cell, Bone, bone marrow cell, Bone Regeneration, Bone repair, cell adhesion, Cell culture, cell differentiation, cell function, cell interaction, cell invasion, Cells, chitosan, Chitosan film, cytokine, Cytokine secretions, Differentiation markers, fibrinogen, gamma interferon, human, Human bone marrow, human cell, Inflammation, Inflammatory response, Mesenchymal Stem Cells, mesenchymal stroma cell, Mesenchymal stromal cells, natural killer cell, Natural killer cells, NK cells, ossification, Pathology, Peripheral blood, Phosphatases, protein expression, Tissue, Tissue regeneration, tissue repair},
	isbn = {17425689 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84856583891\&partnerID=40\&md5=8a6b58ebb9ee4c20099877008acde805},
	author = {Almeida, C.R. and Vasconcelos, D.P. and Gon{\c c}alves, R.M. and Barbosa, M.A.}
}
@article {2214,
	title = {Mesenchymal stem cell recruitment by stromal derived factor-1-delivery systems based on chitosan/poly(γ-glutamic acid) polyelectrolyte complexes},
	journal = {European Cells and MaterialsEur. Cells and Mater.},
	volume = {23},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R. M.; INEB, R. Campo Alegre, 823, Porto 4150-180, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; polyglutamic acid, 24991-23-9, 25513-46-6; Biocompatible Materials; CXCL12 protein, human; Chemokine CXCL12; Chitosan, 9012-76-4; Membranes, Artificial; Polyglutamic Acid, 25513-46-6; poly(gamma-glutamic acid)References: Almeida, C.R., Vasconcelos, D.P., Gon{\c c}alves, R., Barbosa, M.A., Enhanced me s enchymal stromal cell recruitment via Natural Killer cells by incorporation of inflammatory signals in biomaterials (2012) J R Soc Interface, 9, pp. 261-271;Amersham, Iodine-125. A guide to radioiodination techniques (1993) Amersham Life Science, , (I.-A.g.t.r. Techniques, Ed). Amersham, Little Chalfort, UK; 
Antunes, J., Pereira, C.L., Molinos, M., da Ferreira, S.F., Dessi, M., Gloria, A., Ambrosio, L., Barbosa, M.A., Layer-by-layer self-assembly of Chitosan and Poly(γ-glutamic acid) into polyelectrolyte complexes (2011) Biomacromolecules, 12, pp. 4183-4195; 
Barbosa, M., P{\^e}go, A.P., Amaral, I.F., (2011) Chitosan. In: Comprehensive Biomaterials, , 1 st ed (Hutmacher DW, Grainger DW, Healy K, Kirkpatrick CJ, Ducheyne P, eds), Elsevier Amsterdam; 
Bhakta, S., Hong, P., Koc, O., The surface adhesion molecule CXCR4 stimulat es mesenchymal stem cell migration to stromal cell-derived factor-1 in vitro but does not decrease apoptosis under serum deprivation (2006) Cardiovasc Revasc Med, 7, pp. 19-24; 
Bleul, C., Fuhlbrigge, R.C., Casasnovas, J.M., Aiuti, A., Springer, T.A., A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1) (1996) J Exp Med, 184, pp. 110l-1109; 
Boudou, T., Crouzier, T., Ren, K., Blin, G., Picart, C., Multiple functionalities of polyelectrolyte multilayer films: New biomedical applications (2010) Adv Mat, 22, pp. 441-467; 
Broxmeyer, H.E., Christopherson, K.W., Stromal cell derived factor-1/CXCL12, CXCR4 and CD26 in the mobilization and homing of hematopoietic stem and progenitor cells (2004) Curr Med Chem - Anti-Inflammatory \& Anti-Allergy Agents, 3, pp. 303-311; 
Buescher, J., Margaritis, A., Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries (2007) Crit Rev Biotechnol, 27, pp. 1-19; 
Chi, P., Wang, J., Liu, C., Synthesis and characterization of polycationic chitosan-graft-poly (L-lysine) (2008) Mat Lett, 62, pp. 147-150; 
Ciuculescu, F., Giesen, M., Deak, E., Lang, V., Seifried, E., Henschler, R., Variability in chemokine-induced adhesion of human mesenchymal stromal cells (2011) Cytother, 13, pp. 1172-1179; 
Crouzier, T., Ren, K., Nicolas, C., Roy, C., Picart, C., Layer-by-layer films as a biomimetic reservoir for rhBMP-2 delivery: Controlled differentiation of myoblasts to osteoblasts (2009) Small, 5, pp. 598-608; 
de Geest, B., Sanders, N.N., Sukhorukov, G.B., Demeester, J., de Smedt, S.C., Release mechanisms for polyelectrolyte capsules (2007) Chem Soc Rev, 36, pp. 636-649; 
Du, Y.J., Cornelius, R.M., Brash, J.L., Measurement of protein adsorption to gold surface by radioiodination methods: Suppression of free iodide sorption (2000) Colloid Surf B, 17, pp. 59-67; 
Fong, E.L.S., Chan, C.K., Goodman, S.B., Stem cell homing in musculoskeletal injury (2011) Biomaterials, 32, pp. 395-409; 
Frederick, J., Fitzpatrick, J.R., McCormick, R.C., Harris, D.A., Kim, A.Y., Muenzer, J.R., Marotta, N., Woo, Y.J., Stromal cell-derived factor-1alpha activation of tissue-engineered endothelial progenitor cell matrix enhances ventricular function after myocardial infarction by inducing neovasculogenesis (2010) Circulation, 122, pp. S107-S117; 
Gao, H., Priebe, W., Glod, J., Banerjee, D., Activation of signal transducers and activators of transcription 3 and focal adhesion kinase by stromal cell-derived factor 1 is required for migration of human mesenchymal stem cells in response to tumor cell-conditioned medium (2009) Stem Cells, 27, pp. 857-865; 
Gon{\c c}alves, R., Martins, M.C.L., Oliveira, M.J., Almeida-Porada, G., Barbosa, M.A., Bioactivity of immobilized EGF on self-assembled monolayers: Optimization of the immobilization process (2010) J Biomed Mater Res Part A, 94 A, pp. 576-585; 
Granero-Molt{\'o}, F., Weis, J.A., Miga, M.I., Landis, B., Myers, T.J., O{\textquoteright}Rear, L., Longobardi, L., Spagnoli, A., Regenerative effects of transplanted mesenchymal stem cells in fracture healing (2009) Stem Cells, 27, pp. 1887-1898; 
Haider, S., Al-Masry, W.A., Bukhari, N., Javid, M., Preparation of the chitosan containing nanofibers by electrospinning chitosan-gelatin complexes (2010) Polymer Eng Sci, 50, pp. 1887-1893; 
Higuchi, T., Mechanism of sustained-action medication. Theoretical analysis of rate of release of solid drugs dispersed in solid matrices (1963) J Pharm Sci, 52, pp. 1145-1149; 
Hofmann, S., Knecht, S., Langer, R., Kaplan, D.L., Vunjak-Novakovic, G., Merkle, H.P., Meinel, L., Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells (2006) Tissue Eng, 12, pp. 2729-2738; 
Horbett, T., Techniques for protein adsorption studies (1986) Techniques of Biocompatibility Tests, pp. 183-214. , Williams DF, ed), CRC Press, Boca Raton, FL; 
Hsieh, C., Hsieh, H.J., Liu, H.C., Wang, D.M., Hou, L.T., Fabrication and release behavior of a novel freezegelled chitosan/γ-PGA scaffold as a carrier for rhBMP-2 (2006) Dental Mater, 22, pp. 622-629; 
Hsieh, C., Tsai, S.P., Wang, D.M., Chang, Y.N., Hsieh, H.J., Preparation of PGA/chitosan composite tissue engineering matrices (2005) Biomaterials, 26, pp. 5617-5623; 
Huang, M., Vitharana, S.N., Peek, L.J., Coop, T., Berkland, C., Polyelectrolyte complexes stabilize and controllably release Vascular Endothelial Growth Factor (2007) Biomacromolecules, 8, pp. 1607-1614; 
Ichim, T., Alexandrescu, D.U., Solano, F., Lara, F., Campion, R.N., Paris, E., Woods, E.J., Riordan, N.H., Mesenchymal stem cells as anti-inflammatories: Implications for treatment of Duchenne muscular dystrophy (2010) Cell Immunol, 260, pp. 75-82; 
Kim, C., Broxmeyer, H.E., In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: Stromal cell-derived factor-1, Steel factor, and the bone marrow environment (1998) Blood, 91, pp. 100-110; 
Kim, M., Koh, Y.J., Kim, K.E., Koh, B.I., Nam, D.H., Alitalo, K., Kim, I., Koh, G.Y., CXCR4 signaling regulates metastasis of chemoresistant melanoma cells by a lymphatic metastatic niche (2010) Cancer Res, 70, pp. 10411-10421; 
Kuraitis, D.Z.P., Zhang, Y., Padavan, D.T., McEwan, K., Sofrenovic, T., McKee, D., Zhang, J., Suuronen, E.J., Controlled release of stromal cell-derived factor-1 from a collagen matrix for enhanced progenitor cell response and blood vessel growth in ischemia (2011) Eur Cell Mater, 5, pp. 109-123; 
Li, T., Li, H., Wang, Y., Harvard, C., Tan, J.L., Au, A., Xu, Z., You, L., The expression of CXCR4, CXCL12 and CXCR7 in malignant pleural mesothelioma (2011) J Pathol, 223, pp. 519-530; 
Liu, H., Xue, W., Ge, G., Luo, X., Li, Y., Xiang, H., Ding, X., Tian, X., Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1a in MSCs (2010) Biochem Biophys Res Commun, 401, pp. 509-515; 
Macdonald, M., Samuel, R.E., Shah, N.J., Padera, R.F., Beben, Y.M., Hammond, P.T., Tissue integration of growth factor-eluting layer-by-layer polyelectrolyte multilayer coated implants (2011) Biomaterials, 32, pp. 1446-1453; 
Lda, M., Fontes, A.M., Covas, D.T., Caplan, A.I., Mechanisms involved in the therapeutic properties of mesenchymal stem cells (2009) Cytokine Growth Factor Rev, 20, pp. 419-427; 
Mokashi, S.A., Guan, J., Wang, D., Tchantchaleishvili, V., Brigham, M., Lipsitz, S., Lee, L.S., Chen, F.Y., Preventing cardiac remodeling: The combination of cell-based therapy and cardiac support therapy preserves left ventricular function in rodent model of myocardial ischemia (2010) J Thoracic Cardiovasc Surg, 140, pp. 1374-1380; 
Oliveira, S.M., Mijares, D.Q., Turner, G., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., Engineering endochondral bone: In vivo studies (2009) Tissue Eng A, 15, pp. 635-643; 
Peled, A., Petit, I., Kollet, O., Magid, M., Ponomaryov, T., Byk, T., Nagler, A., Lapidot, T., Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4 (1999) Science, 283, pp. 845-848; 
Picart, C., Mutterer, J., Richer, L., Luo, Y., Prestwich, G.D., Schaaf, P., Voegel, J.C., Lavalle, P., Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers (2002) Proc Natl Acad Sci USA, 99, pp. 12531-12535; 
Ponte, A., Marais, E., Gallay, N., Langonne, A., Delorme, B., Herault, E., Charboard, P., Domenesh, J., The in vitro migration capacity of human bone marrow mesenchymal stem cells: Comparison of chemokine and growth factor chemotactic activities (2009) Stem Cells, 25, pp. 1737-1745; 
Rabbany, S., Pastore, J., Yamamoto, M., Miller, T., Rafii, S., Aras, R., Penn, M., Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing (2010) Cell Transplant, 19, pp. 399-408; 
Richert, L., Lavalle, P., Payan, E., Shu, X.Z., Prestwich, G.D., Stoltz, J.F., Schaaf, P., Picart, C., Layer by layer buildup of polysaccharide films: Physical chemistry and cellular adhesion aspects (2003) Langmuir, 20, pp. 448-458; 
Ruster, B., Grace, B., Seitza, O., Seifried, E., Henschler, R., Induction and detection of human mesenchymal stem cell migration in the 48-well reusable Transwell assay (2005) Stem Cells Dev, 14, pp. 231-235; 
Schneider, R.K., Anraths, J., Kramann, R., Bornemann, J., Bovi, M., Kn{\"o}chel, R., Neuss, S., The role of biomaterials in the direction of mesenchymal stem cell properties and extracellular matrix remodelling in dermal tissue engineering (2010) Biomaterials, 31, pp. 7948-7959; 
Shen, W., Chen, X., Chen, J., Yin, Z., Heng, B.C., Chen, W., Ouyang, H.W., The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge in tendon regeneration (2010) Biomaterials, 31, pp. 7239-7249; 
Shinohara, K., Greenfield, S., Pan, H., Vasanji, A., Kumagai, K., Midura, R.J., Kiedrowski, M., Muschler, G.F., Stromal cell-derived factor-1 and monocyte chemotactic protein-3 improve recruitment of osteogenic cells into sites of musculoskeletal repair (2011) J Orthop Res, 29, pp. 1064-1069; 
Singer, N., Caplan, A.I., Mesenchymal stem cells: Mechanisms of inflammation (2011) Annu Rev Pathol Mech Dis, 6, pp. 457-478; 
Song, Z., Yin, J., Luo, K., Zheng, Y., Yang, Y., Li, Q., Yan, S., Chen, X., Layer-by-layer buildup of poly(Lglutamic acid)/chitosan film for biologically active coating (2009) Macromol Biosci, 9, pp. 268-278; 
Sundelacruz, S., Kaplan, D.L., Stem celland scaffold-based tissue engineering approaches to osteochondral regenerative medicine (2009) Sem Cell Dev Biol, 20, pp. 646-655; 
Tang, J., Wang, J.N., Zhang, L., Zheng, F., Yang, J.Y., Kong, X., Guo, L.Y., Chen, S.Y., VEGF/SDF-1 promotes cardiac stem cell mobilization and myocardial repair in the infarcted heart (2011) Cardiovasc Res, 91, pp. 402-411; 
Thevenot, P., Nair, A.M., Shen, J., Lotfi, P., Ko, C.Y., Tang, L., The effect of incorporation of SDF-1a into PLGA scaffolds on stem cell recruitment and the inflammatory response (2010) Biomaterials, 31, pp. 3997-4008; 
Thieme, S., Ryser, M., Gentsch, M., Navratiel, K., Brenner, S., Stiehler, M., Rolfing, J., Rosen-Wolff, A., Stromal cell-derived factor-1a-directed chemoattraction of transiently CXCR4-overexpressing bone marrow stromal cells into functionalized three dimensional biomimetic scaffolds (2009) Tissue Eng C, 15, pp. 687-696; 
Uccelli, A., Moretta, L., Pistoia, V., Mesenchymal stem cells in health and disease (2008) Nat Rev Immunol, 8, pp. 726-736; 
Wu, M.Y., Chen, N., Liu, L.K., Yuan, H., Li, Q.L., Chen, S.H., Chitosan/alginate multilayer scaffold encapsulating bone marrow stromal cells in situ on titanium (2009) J Bioact Compat Polym, 24, pp. 301-315; 
Wynn, R., Hart, C.A., Corradi-Perini, C., O{\textquoteright}Neill, L., Evans, C.A., Wraith, J.E., Fairbairn, L.J., Bellantuono, I., A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow (2004) Blood, 104, pp. 2643-2645; 
Yoo, K., Jang, I.K., Lee, M.W., Kim, H.E., Yang, M.S., Eomb, Y., Lee, J.E., Koo, H.H., Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues (2009) Cellr Immunol, 259, pp. 150-156; 
Zhukareva, V., Obrocka, M., Houle, J.D., Fischer, I., Neuhuber, B., Secretion profile of human bone marrow stromal cells: Donor variability and response to inflammatory stimuli (2010) Cytokine, 50, pp. 317-321
},
	month = {2012///},
	pages = {249 - 261},
	abstract = {Human mesenchymal stem cells (hMSCs) have an enormous potential for tissue engineering and cell-based therapies. With a potential of differentiation into multiple lineages and immune-suppression, these cells play a key role in tissue remodelling and regeneration. Here a method of hMSC recruitment is described, based on the incorporation of a chemokine in Chitosan (Ch)/ Poly(γ-glutamic acid) (γ-PGA) complexes. Ch is a nontoxic, cationic polysaccharide widely investigated. γ-PGA is a hydrophilic, non-toxic, biodegradable and negatively charged poly-amino acid. Ch and γ-PGA, being oppositely charged, can be combined through electrostatic interactions. These biocompatible structures can be used as carriers for active substances and can be easily modulated in order to control the delivery of drugs, proteins, DNA, etc. Using the layer-by-layer method, Ch and γ-PGA were assembled into polyelectrolyte multilayers films (PEMs) with thickness of 120 nm. The chemokine stromal-derived factor-1 (SDF-1) was incorporated in these complexes and was continuously released during 120 h. The method of SDF-1 incorporation is of crucial importance for polymers assembly into PEMs and for the release kinetics of this chemokine. The Ch/γ-PGA PEMs with SDF-1 were able to recruit hMSCs, increasing the cell migration up to 6 fold to a maximum of 16.2 {\textpm} 4.9 cells/mm                        2. The controlled release of SDF-1 would be of great therapeutic value in the process of hMSC homing to injured tissues. This is the first study suggesting Ch/γ-PGA PEMs as SDF-1 reservoirs to recruit hMSCs, describing an efficient method of chemokine incorporation that allows a sustained released up to 5 days and that can be easily scaled-up.
},
	keywords = {adsorption kinetics, article, artificial membrane, Biocompatible Materials, biomaterial, cell differentiation, cell migration, cell motion, Cell Movement, Cell recruitment, chemistry, Chemokine CXCL12, chitosan, controlled study, CXCL12 protein, human, cytology, drug adsorption, drug delivery system, Drug Delivery Systems, drug derivative, drug effect, ellipsometry, enzyme linked immunosorbent assay, human, human cell, human tissue, Humans, in vitro study, isotope labeling, Layer-by-layer, Membranes, Artificial, Mesenchymal stem cell, Mesenchymal Stem Cells, metabolism, methodology, poly(gamma glutamic acid), poly(gamma-glutamic acid), Poly(γ-glutamic acid), polyglutamic acid, Protein delivery, static electricity, stromal cell derived factor 1, sustained drug release},
	isbn = {14732262 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864582297\&partnerID=40\&md5=2fe4a066582cc820d2a8f9dd64fde21b},
	author = {Gon{\c c}alves, R.M. and Antunes, J.C. and Barbosa, M.A.}
}
@article {2291,
	title = {Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2009.09.010PubMed ID: 19783294Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R.M.; INEB, Biomaterials Division, NEWTherapies Group, R. Campo Alegre, 823, 4150-180 Porto, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: Calcium-Binding Proteins; Gold, 7440-57-5; Immunoglobulin G; Intercellular Signaling Peptides and Proteins; Membrane Proteins; Receptors, Fc; Receptors, Notch; Recombinant Fusion Proteins; Serrate proteins, 134324-36-0References: Artavanis-Tsakonas, S., Rand, M.D., Lake, R.J., Notch signaling: cell fate control and signal integration in development (1999) Science, 284, pp. 770-776;Leong, K., Karsan, A., Recent insights in the role of notch signaling in tumorigenesis (2006) Blood, 107 (6), pp. 2223-2233; 
Chiba, S., Concise review: notch signaling in stem cell systems (2006) Stem Cells, 24, pp. 2437-2447; 
Lowell, S., Benchoua, A., Heavey, B., Smith, A.G., Notch promotes neural lineage entry by pluripotent embryonic stem cells (2006) PLoS Biol, 4 (5); 
Hellstr{\"o}m, M.P.L., Hofmann, J.J., Wallgard, E., Coultas, L., Lindblom, P., Alva, J., Dll4 signalling through notch1 regulates formation of tip cells during angiogenesis (2007) Nature, 15 (445), pp. 776-780; 
Hofmeister, C., Zhang, J., Knight, K.L., Le, P., Stiff, P.J., Ex vivo expansion of umbilical cord blood stem cells for transplantation: growing knowledge from the hematopoietic niche (2007) Bone Marrow Transplant, 39 (1), pp. 11-23; 
Hilton, M., Tu, X., Wu, X., Bai, S., Zhao, H., Kobayashi, T., F. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation (2008) Nat Med, 14 (3), pp. 306-314; 
Stylianou, S., Clarke, R.B., Brennan, K., Aberrant activation of notch signaling in human breast cancer (2006) Cancer Res, 66 (3), pp. 1517-1525; 
Demarest, R., Ratti, F., Capobianco, A.J., It{\textquoteright}s T-ALL about notch (2008) Oncogene, 27 (38), pp. 5082-5091; 
Fre, S., Huyghe, M., Mourikis, P., Robine, S., Louvard, D., Artavanis-Tsakonas, S., Notch signals control the fate of immature progenitor cells in the intestine (2005) Nature, 16 (435), pp. 964-968; 
Fischer, A., Gessler, M., Delta-notch-and then? Protein interactions and proposed modes of repression by Hes and Hey bHLH factors (2007) Nucleic Acids Res, 35 (14), pp. 4583-4596; 
Zweidler-McKay, P.A., He, Y., Xu, L., Rodriguez, C.G., Karnell, F.G., Carpenter, A.C., Notch signaling is a potent inducer of growth arrest and apoptosis in a wide range of B-cell malignancies (2005) Blood, 106 (12), pp. 3898-3906; 
Beckstead, B., Santosa, D.M., Giachelli, C.M., Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation (2006) J Biomed Mater Res, 79 A, pp. 94-103; 
Varnum-Finney, B., Wu, L., Yu, M., Brashem-Stein, C., Staats, S., Flowers, D., Immobilization of Notch ligand, Delta-1, is required for induction of notch signaling (2000) J Cell Sci, 113 (23), pp. 4313-4318; 
Delaney, C., Varnum-Finney, B., Aoyama, K., Brashem-Stein, C., Bernstein, I.D., Dose-dependent effects of the notch ligand delta1 on ex vivo differentiation and in vivo marrow repopulating ability of cord blood cells (2005) Blood, 106, pp. 2693-2699; 
Lefort, N., Bennea, C., Leli{\`e}vrea, J.D., Dorivala, C., Balboa, M., Sakanoc, S., Short exposure to notch ligand delta-4 is sufficient to induce T-cell differentiation program and to increase the T cell potential of primary human CD34+ cells (2006) Exp Hematol, 34, pp. 1720-1729; 
Taqvi, S., Dixit, L., Krishnendu, R., Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells (2006) J Biomed Mater Res, 79 A, pp. 689-697; 
Bernstein, I., Delaney, C., Dallas, M., Heimfeld, S., Varnum-Finney, B., Notch signaling in hematopoiesis: implication for improving stem cell transplantation (2008) Biol Blood Marrow Transplant, 14, pp. 94-99; 
De Smedt, M., Hoebeke, I., Reyvoet, K., Leclercq, G., Plum, J., Different thresholds of~Notch signaling bias human precursor cells toward B-, NK-, monocytic/dendritic-, or T-cell lineage in thymus microenvironment (2005) Blood, 106, pp. 3498-3506; 
Mrksich, M., What can surface chemistry do for cell biology? (2002) Curr Opin Chem Biol, 6, pp. 794-797; 
France, R., Short, R.D., Dawsonb, R.A., MacNeilb, S., Attachment of human keratinocytes to plasma copolymers of acrylic acid/octa-1,7-diene and allyl amine/octa-1,7-diene (1998) J Mater Chem, 81 (1), pp. 37-42; 
Neves, H., Weerkamp, F., Gomes, A.C., Naber, B., Gameiro, P., Becker, J.L., Effects of delta1 and jagged1 on early human hematopoiesis: correlation with expression of notch signaling-related genes in CD34+ Cells (2006) Stem Cells, 24, pp. 1328-1337; 
Sokolova, V., Epple, M., Inorganic nanoparticles as carriers of nucleic acids into cells (2008) Angewandte Chemie, 47, pp. 1382-1395; 
Yao, J., Duan, L., Fan, M., Yuan, J., Wu, X., Notch1 induces cell cycle arrest and apoptosis in human cervical cancer cells: involvement of nuclear factor kappa B inhibition (2007) Int J Gynecol Cancer, 17, pp. 502-510; 
Chadwick, N., Nostro, M.C., Baron, M., Mottram, R., Brady, G., Buckle, A., Notch signaling induces apoptosis in primary human CD34+ hematopoietic progenitor cells (2007) Stem Cells, 25, pp. 203-210; 
Love, J., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1169; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Martins, M.C.L., Ratner, B., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl- terminated self-assembled monolyers (2003) J Biomed Mater Res, 67 (A), pp. 158-171; 
Martins, M.C.L., Curtins, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res A, 88 (1), pp. 162-173; 
Barrias, C.C., Martins, M.C.L., Bidarra, S., S{\'a} Miranda, M.C., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: effect of the surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
Iodine-125. A guide to radioiodination techniques. In: techniques I-Agtr, ed.: Little Chalfort: Amersham Life Science 1993Horbett, T., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, pp. 183-214. , DF W. (Ed), CRC Press Inc, Harcover; 
Ohishi, K., Varnum-Finney, B., Bernstein, I.D., Delta-1 enhances marrow and thymus repopulating ability of human CD34+ CD38- cord blood cells (2002) J Clin Invest, 110, pp. 1165-1174; 
Li, L., Chen, S., Zheng, J., Ratner, B.D., Jiang, S., Protein adsorption on oligo(ethylene glycol)-terminated alkanethiolate self-assembled monolayers: the molecular basis for nonfouling behavior (2005) J Phys Chem B, 109 (7), pp. 2934-2941; 
Tohda, S., Murata-Ohsawa, M., Sakano, S., Nara, N., Notch ligands, Delta-1 and Delta-4 supress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines (2003) Int J Oncol, 22 (5), pp. 1073-1079; 
Tohda, S.K.H., Murakami, N., Sakano, S., Nara, N., Diverse effects of the Notch ligands Jagged1 and Delta1 on the growth and differentiation of primary acute myeloblastic leukemia cells (2005) Exp Hematol, 33, pp. 558-563; 
Aoyama, K., Delaney, C., Varnun-Finney, B., Kohn, A.D., Moon, R.T., Bernstein, I.D., The interaction of the Wnt and notch pathways modulates natural killer versus T cell differentiation (2007) Stem Cells, 25, pp. 2488-2497; 
Beckstead B, Tung JC, Liang KJ, Tavakkol Z, Usui ML, Olerud JE, et~al. Methods to promote notch signaling at the biomaterial interface and evaluation in a rafted organ culture model. J Biomed Mater Res A, in press. [Epub ahead of print]Semler, E., Dasgupta, A., Moghe, P.V., Cytomimetic engineering of hepatocyte morphogenesis and function by substrate-based presentation of acellular E-cadherin (2005) Tissue Eng, 11 (5-6), pp. 734-750; 
Zhao, Z., Matsui, H., Accurate immobilization of antibody-functionalized peptide nanotubes on protein-patterned arrays by optimizing their ligand-receptor interactions (2007) Small, 3 (8), pp. 1390-1393; 
Longo, G., Szleifer, Ligand-receptor interactions in tethered polymer layers (2005) Langmuir, 21, pp. 11342-11351; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption and clotting time of pHEMA hydrogels modified with C18 ligands to adsorb albumin selectively and reversibly (2009) Biomaterials, 30 (29), pp. 5541-5551
},
	month = {2009///},
	pages = {6879 - 6887},
	abstract = {Notch signaling is a key mechanism during mammal development and stem cell regulation. This study aims to target and control Notch signaling by ligands immobilization using self-assembled monolayers (SAMs) as model surfaces. Non-fouling substrates were prepared by immersion of gold substrates in (1-Mercapto-11-undecyl)tetra(ethylene glycol) thiol solutions. These surfaces were activated with N,N'-carbonyldiimidazole (CDI) at different concentrations (0, 0.03, 0.3, 3 and 30 mg/ml) and an anti-human IgG, Fc specific fragment antibody (Ab) was covalently bound to EG4-SAMs to guarantee the correct exposure of the Notch ligand Jagged-1/Fc chimera (Jag-1). The presence of Ab and Jag-1 was confirmed by radiolabeling, X-ray photoelectron spectroscopy (XPS), ellipsometry and ELISA. The biological activity of Jag-1-Ab-SAMs was assessed by real-time PCR for Hes-1 family gene expression, a Notch pathway target gene, in HL-60 cell line. Results have shown an increase of the amount of immobilized Ab with increasing surface activator concentrations. Jag-1 concentration also increases with Ab concentration. Interestingly, a higher Jagged-1 exposure and fold increase in Hes-1 expression were obtained for surfaces activated with the lowest concentration of CDI (0.03 mg/ml). These results illustrate the great importance of ligands orientation and exposure, when compared with density. This investigation brings new insights into Notch signaling mechanisms. In particular, Jag-1-Ab-SAMs have shown to be adequate model surfaces to study Notch pathway activation and may provide a basis to develop new interfaces in biomaterials to control Notch mechanism in different cell systems. {\textcopyright} 2009 Elsevier Ltd.},
	keywords = {Activator concentration, Adequate models, Adsorption, Animals, article, Bioactivity, Biological activities, Biological materials, biomaterial, Calcium-Binding Proteins, Carbonyldiimidazole, Cell culture, Cell Line, Cell membranes, cell strain HL 60, Cell system, Chemical activation, Concentration (process), Concentration of, controlled study, Covalently bound, Ethylene, Ethylene Glycol, gene expression, gold, Gold substrates, HL-60 cell line, human, human cell, Human IgG, Humans, Immunoassay, immunoglobulin Fc fragment, Immunoglobulin G, immunoglobulin G antibody, Intercellular Signaling Peptides and Proteins, Jagged1, Ligands, Mammalia, Membrane Proteins, Model surface, Molecular Structure, Nanostructured materials, Nanostructured surfaces, Nonfouling, Notch receptor, Notch signaling, Photoelectron spectroscopy, priority journal, Protein adsorption, protein immobilization, Radiolabeling, Real-time PCR, Receptors, Fc, Receptors, Notch, Recombinant Fusion Proteins, Sams, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, signal transduction, Signaling, stem cell, Surface Properties, Target genes, Tetra, transcription factor HES 1, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70350188310\&partnerID=40\&md5=6c55d29cf8ae55288f3e87880171ec70},
	author = {Gon{\c c}alves, R.M. and Martins, M.C.L. and Almeida-Porada, G. and Barbosa, M.A.}
}
