



@article {2241,
	title = {The Arabidopsis D-Type Cyclin CYCD2;1 and the Inhibitor ICK2/KRP2 Modulate Auxin-Induced Lateral Root Formation},
	journal = {Plant CellPlant Cell},
	volume = {23},
	year = {2011},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: ScopusCODEN: PLCEEdoi: 10.1105/tpc.110.080002PubMed ID: 21357490Language of Original Document: EnglishCorrespondence Address: Murray, J. A. H.; Cardiff School of Biosciences, Cardiff University, CF10 3AX Cardiff, United Kingdom; email: murrayja1@cardiff.ac.ukChemicals/CAS: proteasome, 140879-24-9; ATP dependent 26S protease, 3.4.99.-; Arabidopsis Proteins; Cell Cycle Proteins; CycD2 protein, Arabidopsis; Cyclins; ICK2 protein, Arabidopsis; Indoleacetic Acids; Proteasome Endopeptidase Complex, 3.4.25.1References: Achard, P., Gusti, A., Cheminant, S., Alioua, M., Dhondt, S., Coppens, F., Beemster, G.T., Genschik, P., Gibberellin signaling controls cell proliferation rate in Arabidopsis (2009) Curr. Biol., 19, pp. 1188-1193;Alonso, J.M., Genome-wide insertional mutagenesis of Arabidopsis thaliana (2003) Science, 301, pp. 653-657; 
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},
	month = {2011///},
	pages = {641 - 660},
	abstract = {The integration of cell division in root growth and development requires mediation of developmental and physiological signals through regulation of cyclin-dependent kinase activity. Cells within the pericycle form de novo lateral root meristems, and D-type cyclins (CYCD), as regulators of the G                        1-to-S phase cell cycle transition, are anticipated to play a role. Here, we show that the D-type cyclin protein CYCD2;1 is nuclear in Arabidopsis thaliana root cells, with the highest concentration in apical and lateral meristems. Loss of CYCD2;1 has a marginal effect on unstimulated lateral root density, but CYCD2;1 is rate-limiting for the response to low levels of exogenous auxin. However, while CYCD2;1 expression requires sucrose, it does not respond to auxin. The protein Inhibitor-Interactor of CDK/Kip Related Protein2 (ICK2/KRP2), which interacts with CYCD2;1, inhibits lateral root formation, and ick2/krp2 mutants show increased lateral root density. ICK2/ KRP2 can modulate the nuclear levels of CYCD2;1, and since auxin reduces ICK2/KRP2 protein levels, it affects both activity and cellular distribution of CYCD2;1. Hence, as ICK2/KRP2 levels decrease, the increase in lateral root density depends on CYCD2;1, irrespective of ICK2/CYCD2;1 nuclear localization. We propose that ICK2/KRP2 restrains root ramification by maintaining CYCD2;1 inactive and that this modulates pericycle responses to auxin fluctuations. {\textcopyright} 2011 American Society of Plant Biologists.
},
	keywords = {Arabidopsis, Arabidopsis protein, Arabidopsis Proteins, Arabidopsis thaliana, article, ATP dependent 26S protease, cell cycle, cell cycle protein, Cell Cycle Proteins, cell nucleus, CycD2 protein, Arabidopsis, cycline, Cyclins, gene expression regulation, Gene Expression Regulation, Plant, genetics, growth, development and aging, ICK2 protein, Arabidopsis, indoleacetic acid derivative, Indoleacetic Acids, metabolism, mutation, plant root, Plant roots, proteasome, Proteasome Endopeptidase Complex},
	isbn = {10404651 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79953123085\&partnerID=40\&md5=f1355f64db2bcf9f2d881104f23005eb},
	author = {Sanz, L. and Dewitte, W. and Forzani, C. and Patell, F. and Nieuwland, J. and Wen, B. and Quelhas, P. and de Jager, S. and Titmus, C. and Campilho, A. and Ren, H. and Estelle, M. and Wang, H. and Murray, J.A.H.}
}
@book {2222,
	title = {Arabidopsis thaliana automatic cell file detection and cell length estimation},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6754 LNCS},
	year = {2011},
	note = {Conference code: 85546Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21596-4_1Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; INEB - Divis{\~a}o de Sinal e Imagem, Instituto de Engenharia Biom{\'e}dica, Porto, PortugalReferences: Terpstra, I., Heidstra, R., Stem cells: The root of all cells (2009) Semin. Cell Dev. Biol., 20 (9), pp. 1089-1096;Bennett, T., Scheres, B., Root development-two meristems for the price of one? (2010) Curr. Top Dev. Bio., 91, pp. 67-102; 
De Smet, I., Tetsumura, T., De Rybel, B., Frey, N.F., Laplaze, L., Casimiro, I., Swarup, R., Beeckman, T., Auxin-dependent regulation of lateral root positioning in the basal meristem of arabidopsis (2007) Development, 134 (4), pp. 681-690; 
Nieuwland, J., Maughan, S., Dewitte, W., Scofield, S., Sanz, L., Murray, J.A., The d-type cyclin cycd4;1 modulates lateral root density in arabidopsis by affecting the basal meristem region (2009) Proc. Natl. Acad. Sci. U S A, 106 (52), pp. 22528-22533; 
Vanstraelen, M., Baloban, M., Da Ines, O., Cultrone, A., Lammens, T., Boudolf, V., Brown, S.C., Kondorosi, E., Apc/c-ccs52a complexes control meristem maintenance in the arabidopsis root (2009) Proc. Natl. Acad. Sci. U S A, 106 (28), pp. 11806-11811; 
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Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., Automated arabidopsis plant root cell segmentation based on svm classification and region merging (2009) Comp. in Biology and Medicine, 39, pp. 785-793; 
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},
	month = {2011///},
	pages = {1 - 11},
	address = {Burnaby, BC},
	abstract = {In plant development biology, the study of the structure of the plant{\textquoteright}s root is fundamental for the understanding of the regulation and interrelationships of cell division and cellular differentiation. This is based on the high connection between cell length and progression of cell differentiation and the nuclear state. However, the need to analyse a large amount of images from many replicate roots to obtain reliable measurements motivates the development of automatic tools for root structure analysis. We present a novel automatic approach to detect cell files, the main structure in plant roots, and extract the length of the cells in those files. This approach is based on the detection of local cell file characteristic symmetry using a wavelet based image symmetry measure. The resulting detection enables the automatic extraction of important data on the plant development stage and of characteristics for individual cells. Furthermore, the approach presented reduces in more than 90\% the time required for the analysis of each root, improving the work of the biologist and allowing the increase of the amount of data to be analysed for each experimental condition. While our approach is fully automatic a user verification and editing stage is provided so that any existing errors may be corrected. Given five test images it was observed that user did not correct more than 20\% of all automatically detected structure, while taking no more than 10\% of manual analysis time to do so. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Arabidopsis thaliana, Automatic extraction, Automatic tools, Bio-imaging, BioImaging, cell differentiation, Cell divisions, Cell lengths, cell proliferation, Cells, Cellular differentiation, Experimental conditions, Image analysis, image symmetry, Individual cells, Main structure, Manual analysis, Nuclear state, Plant development, Plant roots, Plants (botany), Reliable measurement, Root structure, Test images, User verification, Wavelet analysis, Wavelet-based images},
	isbn = {03029743 (ISSN); 9783642215957 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960337749\&partnerID=40\&md5=ef49700082788511730e38d4eda9a566},
	author = {Quelhas, P. and Nieuwland, J. and Dewitte, W. and Mendon{\c c}a, A.M. and Murray, J. and Campilho, A.}
}
