



@article {3293,
	title = {The predominance of M2-polarized macrophages in the stroma of low-hypoxic bladder tumors is associated with BCG immunotherapy failure},
	journal = {Urologic Oncology: Seminars and Original Investigations},
	volume = {32},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {449 - 457},
	abstract = {Objective: Bacillus Calmette-Gu{\'e}rin (BCG) immunotherapy is the gold standard treatment for superficial bladder tumors with intermediate/high risk of recurrence or progression. However, approximately 30\% of patients fail to respond to the treatment. Effective BCG therapy needs precise activation of the type 1 helper cells immune pathway. Tumor-associated macrophages (TAMs) often assume an immunoregulatory M2 phenotype and may directly interfere with the BCG-induced antitumor immune response. Thus, we aim to clarify the influence of TAMs, in particular of the M2 phenotype in stroma and tumor areas, in BCG treatment outcome. Patients and methods: The study included 99 patients with bladder cancer treated with BCG. Tumors resected before treatment were evaluated using immunohistochemistry for CD68 and CD163 antigens, which identify a lineage macrophage marker and a M2-polarized specific cell surface receptor, respectively. CD68+ and CD163+ macrophages were evaluated within the stroma and tumor areas, and high density of infiltrating cells spots were selected for counting. Hypoxia, an event known to modulate macrophage phenotype, was also assessed through hypoxia induced factor (HIF)-1α expression. Results: Patients in whom BCG failed had high stroma-predominant CD163+ macrophage counts (high stroma but low tumor CD163+ macrophages counts) when compared with the ones with a successful treatment (71\% vs. 47\%, P = 0.017). Furthermore, patients presenting this phenotype showed decreased recurrence-free survival (log rank, P = 0.008) and a clear 2-fold increased risk of BCG treatment failure was observed in univariate analysis (hazard ratio = 2.343; 95\% CI: 1.197-4.587; P = 0.013). Even when adjusted for potential confounders, such as age and therapeutic scheme, multivariate analysis revealed 2.6-fold increased risk of recurrence (hazard ratio = 2.627; 95\% CI: 1.340-5.150; P = 0.005). High stroma-predominant CD163+ macrophage counts were also associated with low expression of HIF-1α in tumor areas, whereas high counts of CD163+ in the tumor presented high expression of HIF-1α in tumor nests. Conclusions: TAMs evaluation using CD163 is a good indicator of BCG treatment failure. Moreover, elevated infiltration of CD163+ macrophages, predominantly in stroma areas but not in the tumor, may be a useful indicator of BCG treatment outcome, possibly owing to its immunosuppressive phenotype. {\textcopyright} 2014 Elsevier Inc.},
	keywords = {BCG immunotherapy, Bladder cancer, CD163, CD68, Tumor-associated macrophages},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84899473274\&partnerID=40\&md5=4716f90a43e05e83d41df6d643097edd},
	author = {Lima, L. and Oliveira, D. and Tavares, A. and Amaro, T. and Cruz, R. and Oliveira, M.J. and Ferreira, J.A. and Santos, L.}
}
@article {2865,
	title = {Overexpression of tumour-associated carbohydrate antigen sialyl-Tn in advanced bladder tumours},
	journal = {Molecular Oncology},
	volume = {7},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {719 - 731},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84878107131\&partnerID=40\&md5=52c1f1e8bc83224d947840df0f3dd539},
	author = {Ferreira, J.A. and Videira, P.A. and Lima, L. and Pereira, S. and Silva, M. and Carrascal, M. and Severino, P.F. and Fernandes, E. and Almeida, A. and Costa, C. and Vitorino, R. and Amaro, T. and Oliveira, M.J. and Reis, C.A. and Dall{\textquoteright}Olio, F. and Amado, F. and Santos, L.L.}
}
@article {2233,
	title = {The effect of slurry preparation methods on biaxial flexural strength of dental porcelain},
	journal = {Journal of Prosthetic DentistryJ. Prosthet. Dent.},
	volume = {105},
	year = {2011},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JPDEAdoi: 10.1016/S0022-3913(11)60058-9PubMed ID: 21530756Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; Institute of Biomedical Engineering, Rua do Campo Alegre, 283, 4150-180 Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: Complex Mixtures; Dental Porcelain, 12001-21-7References: The Glossary of Prosthodontic Terms (2005) J Prosthet Dent, 94, pp. 10-92;(2008) Dentistry - Ceramic Materials, , ISO 6872 Geneva, Switzerland: International Organization for Standards; 
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},
	month = {2011///},
	pages = {308 - 314},
	abstract = {Statement of problem. One-step and incremental mixing procedures are currently used to produce dental ceramic pastes. In the ceramic industry, high quality is obtained using one-step mixing, but in dentistry, the best method has not been yet determined. Purpose. The purpose of this study is to evaluate the effects of 2 mixing techniques on the biaxial flexural strength and microstructure of dental porcelain. Material and methods. Feldspathic porcelain discs (2 {\texttimes} 15 mm in diameter) were produced and divided according to the ceramic paste preparation method, powder-liquid incremental mixing group (n=50) or one-step mixing, as a control group (n=50). Specimens were tested for biaxial flexural strength and characterized using porosimetry, relative humidity, SEM/EDS, XRD, and FT-IR analyses. Statistical analysis was conducted using Weibull statistics. The Weibull modulus, characteristic strength and relative humidity were compared between groups, using Student{\textquoteright}s t-test and Mann-Whitney U test (α=.05). Results. The powder-liquid incremental mixing group showed significantly higher values (SD) of Weibull modulus (6.74 (0.70), P<.001) and characteristic strength (79.87 (2.01) MPa, P<.001) when compared to the one-step mixing group (4.94 (0.94) and 75.95 (2.61) MPa). Significantly lower mean (SD) relative humidity values (P=.009) were found for powder-liquid incremental mixing group (20\% (0.5\%)) compared to one-step mixing group (22\% (1\%)). XRD spectra showed that the one-step mixing group produced higher amounts of the amorphous phase. Conclusions. Specimens produced by the incremental mixing technique showed higher biaxial flexural strength than one-step mixing. {\textcopyright} 2011 The Editorial Council of the Journal of Prosthetic Dentistry.},
	keywords = {article, chemistry, comparative study, Complex Mixtures, Crowns, dental care, Dental Porcelain, Dental Prosthesis Design, Dental Stress Analysis, Dental Veneers, denture, drug mixture, evaluation, materials testing, pliability, tooth crown, tooth prosthesis},
	isbn = {00223913 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955604181\&partnerID=40\&md5=53c0732c254214b4a53e3222fe89f4a0},
	author = {Pelaez-Vargas, A. and Dussan, J.A. and Restrepo-Tamayo, L.F. and Paucar, C. and Ferreira, J.A. and Monteiro, F.J.}
}
