



@article {3272,
	title = {Cellular strategies to promote vascularisation in tissue engineering applications},
	journal = {European Cells and Materials},
	volume = {28},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {51 - 67},
	abstract = {Vascularisation is considered to be one of the greatest challenges in tissue engineering. Different strategies exist but cell-based approaches have emerged as a promising therapy to achieve successful vascularisation. The use of endothelial cells to engineer vascularised tissues has been extensively investigated. This field of research has evolved with the discovery of endothelial progenitor cells, a subpopulation with a high regenerative potential. However, the survival of endothelial cell populations alone seems to be impaired. To overcome this problem, co-culture systems, involving supporting cells, like mural cells, fibroblasts, or more tissue-specific cells have been developed. Endothelial cells benefit from the extracellular matrix components and growth factors produced by the supporting cells, which results in neovessel stabilisation and maturation. The use of endothelial progenitor cells in co-culture systems appears to be a promising strategy to promote vascularisation in approaches of increasing complexity. Herein, the authors provide an overview of the cellular strategies that can be used for increasing vascularisation in tissue engineering and regeneration.},
	keywords = {angiogenesis, Cellular therapies, Co-culture models, Endothelial progenitor cells, Regeneration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904957526\&partnerID=40\&md5=dbd4f3056e55c7dc52bc526ea7eb7d69},
	author = {Costa-Almeida, R. and Granja, P.L. and Soares, R. and Guerreiro, S.G.}
}
@article {3291,
	title = {Neonatal human dermal fibroblasts immobilized in RGD-alginate induce angiogenesis},
	journal = {Cell Transplantation},
	volume = {23},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {945 - 957},
	abstract = {Promoting angiogenesis in a damaged tissue is a major challenge for tissue regeneration. Recent findings in tissue engineering suggest that fibroblasts (FBs) play an important role in orchestrating the angiogenic process. Fibroblasts maintain the structural integrity of connective tissue by continuously secreting growth factors and extracellular matrix precursors, which are essential for endothelial cell (EC) adhesion and spreading, thus playing a crucial role in angiogenesis. We hypothesized that FBs immobilized in alginate gels grafted with the RGD peptidic sequence could influence the recruitment of ECs to improve vascularization. In this work, the modulation of immobilized human FBs within the 3D synthetic extracellular matrix was assessed. Experiments using cocultures of ECs and FBs in indirect contact as well as angiogenic assays were performed to assess the influence of FBs immobilized in RGD-alginate in ECs{\textquoteright} viability, stabilization, sprouting, and assembly into capillary-like structures. This study demonstrates the ability of FBs immobilized within RGD-alginate microspheres to modulate and support capillary-like structures{\textquoteright} assembly. These findings indicate that the microenvironment created by these stromal cells in the scaffold modulates capillary morphogenesis, thus stimulating angiogenesis in situ and can potentially be used in regenerative medicine in clinical scenarios where vascularization is essential. {\textcopyright} 2014 Cognizant Comm. Corp.},
	keywords = {Alginate, angiogenesis, Endothelial cells (ECs), Fibroblasts (FBs), RGD peptide: Injectable biomaterials},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905231059\&partnerID=40\&md5=8ca92aea469585cbf168cb6fbad9d3ad},
	author = {Guerreiro, S.G. and Oliveira, M.J. and Barbosa, M.A. and Soares, R. and Granja, P.L.}
}
@article {2237,
	title = {Pectin-based injectable biomaterials for bone tissue engineering},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {12},
	year = {2011},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm101110xPubMed ID: 21302960Language of Original Document: EnglishCorrespondence Address: Munarin, F.; Biomatlab, Bioengineering Department, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milan, Italy; email: fabiola.munarin@mail.polimi.itChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; osteocalcin, 136461-80-8; pectin, 9000-69-5; Biocompatible Materials; Oligopeptides; Pectins; arginyl-glycyl-aspartic acid, 99896-85-2; pectin, 9000-69-5References: Brown, M.J., Lester, J.N., (1980) Appl. Environ. Microbiol., 40, pp. 179-185;Knox, J.P., (2008) Curr. Opin. Plant Biol., 1, pp. 308-313; 
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},
	month = {2011///},
	pages = {568 - 577},
	abstract = {A variety of natural polymers and proteins are considered to be 3D cell culture structures able to mimic the extracellular matrix (ECM) to promote bone tissue regeneration. Pectin, a natural polysaccharide extracted from the plant cell walls and having a chemical structure similar to alginate, provides interesting properties as artificial ECM. In this work, for the first time, pectin, modified with an RGD-containing oligopeptide or not, is used as an ECM alternative to immobilize cells for bone tissue regeneration. The viability, metabolic activity, morphology, and osteogenic differentiation of immobilized MC3T3-E1 preosteoblats demonstrate the potential of this polysaccharide to keep immobilized cells viable and differentiating. Preosteoblasts immobilized in both types of pectin microspheres maintained a constant viability up to 29 days and were able to differentiate. The grafting of the RGD peptide on pectin backbone induced improved cell adhesion and proliferation within the microspheres. Furthermore, not only did cells grow inside but also theywere able to spread out from themicrospheres and to organize themselves in 3D structures producing a mineralized extracellular matrix. These promising results suggest that pectin can be proposed as an injectable cell vehicle for bone tissue regeneration. {\textcopyright} 2011 American Chemical Society.},
	keywords = {3-D Cell culture, 3D Structure, 3T3 Cells, Adhesion, Alginate, animal cell, Animals, arginylglycylaspartic acid, article, Biocompatible Materials, Biopolymers, Bone, Bone and Bones, Bone Regeneration, bone tissue, Bone tissue engineering, Bone tissue regeneration, carbohydrate analysis, cell adhesion, Cell culture, cell differentiation, cell growth, cell metabolism, cell proliferation, cell spreading, cell structure, cell viability, Cells, Chemical modification, Chemical structure, controlled study, Cryoelectron Microscopy, Extracellular matrices, extracellular matrix, gene expression, immobilized cell, Immobilized cells, Injectable biomaterials, Injections, MC3T3-E1, Metabolic activity, Mice, Microscopy, Electron, Scanning, microsphere, Microspheres, Mineralized extracellular matrixes, mouse, Natural polysaccharide, nonhuman, Oligopeptides, osteoblast, osteocalcin, Osteogenic differentiation, pectin, Pectins, Plant cell culture, Plant cell wall, Preosteoblasts, priority journal, RGD peptide, Three dimensional, Tissue engineering},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955409005\&partnerID=40\&md5=277a7d5e91116f9a03f144aabda0dd96},
	author = {Munarin, F. and Guerreiro, S.G. and Grellier, M.A. and Tanzi, M.C. and Barbosa, M.A. and Petrini, P. and Granja, P.L.}
}
