



@article {2243,
	title = {Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping},
	journal = {Microscopy and MicroanalysisMicrosc. Microanal.},
	volume = {16},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: MIMIFdoi: 10.1017/S1431927610094158PubMed ID: 20964878Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Faculdade de Engenharia, Departamento de Engenharia Metalrgica e Materiais, Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; Silicon Dioxide, 7631-86-9References: Albrektsson, T., Sennerby, L., Wennerberg, A., State of the art of oral implants (2000) Periodontology, 47, pp. 15-26;Albrektsson, T., Wennerberg, A., Oral implant surfaces: Part 1-Review focusing on topographic and chemical properties of different surfaces and in vivo responses to them (2004) Int J Prosthodont, 17 (5), pp. 536-543; 
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},
	month = {2010///},
	pages = {670 - 676},
	abstract = {While there has been rapid development of microfabrication techniques to produce high-resolution surface modifications on a variety of materials in the last decade, there is still a strong need to produce novel alternatives to induce guided tissue regeneration on dental implants. High-resolution microscopy provides qualitative and quantitative techniques to study cellular guidance in the first stages of cell-material interactions. The purposes of this work were (1) to produce and characterize the surface topography of isotropic and anisotropic microfabricated silica thin films obtained by sol-gel processing, and (2) to compare the in vitro biological behavior of human bone marrow stem cells on these surfaces at early stages of adhesion and propagation. The results confirmed that a microstamping technique can be used to produce isotropic and anisotropic micropatterned silica coatings. Atomic force microscopy analysis was an adequate methodology to study in the same specimen the sintering derived contraction of the microfabricated coatings, using images obtained before and after thermal cycle. Hard micropatterned coatings induced a modulation in the early and late adhesion stages of cell-material and cell-cell interactions in a geometry-dependent manner (i.e., isotropic versus anisotropic), as it was clearly determined, using scanning electron and fluorescence microscopies. {\textcopyright} Microscopy Society of America 2010.},
	keywords = {AFM, Atomic force microscopy, audiovisual equipment, cell adhesion and proliferation, Cell Culture Techniques, cell proliferation, conference paper, culture technique, cytology, Fluorescence microscopy, Guided tissue regeneration, human, Humans, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Microscopy, Fluorescence, microstamping, Motion Pictures as Topic, myeloid progenitor cell, Myeloid Progenitor Cells, Scanning electron microscopy, SEM, silica sol-gel films, silicon dioxide, Soft lithography, Surface Properties, surface property, Tissue regeneration, tissue scaffold, Tissue Scaffolds},
	isbn = {14319276 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79451471917\&partnerID=40\&md5=c68d4037bb3535a092d46c2602a8b4bd},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Ferrell, N. and Fernandes, M.H. and Hansford, D. and Monteiro, F.J.}
}
@book {2319,
	title = {Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {396-398},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAEdoi: 10.4028/0-87849-353-0.303Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do PortoPortugal; email: alejopv@ineb.up.ptReferences: Warashina, H., Sakano, S., Kitamura, S., Yamauchi, K.I., (2003) Biomaterials, 24, pp. 3655-3661;Scarano, A., Piattelli, M., Caputi, S., Favero, G.A., Piattelli, A., (2004) J Periodontol, 75, pp. 292-296; 
Manicone, P.F., Rossi Iommetti, P., Raffaelli, L., (2007) J Dent, 35, pp. 819-826; 
Covacci, V., Bruzzese, N., Maccauro, G., Andreassi, C., (1999) Biomaterials, 20, pp. 371-376; 
Silva, V.V., Lameiras, F.S., Lobato, Z.I., (2002) J Biomed Mater Res, 63, pp. 583-590; 
Oliva, J., Oliva, X., Oliva, J., (2007) Int J Oral Maxillofac Implants, 22, pp. 430-435; 
Klinge, B., Hultin, M., Berglundh, T., (2005) Dent Clin North Am, 49, pp. 661-676; 
Wennerberg, A., Albrektsson, T., Andersson, B., Krol, J.J., (1995) Clin Oral Impl Res, 6, pp. 24-30; 
Brunette, D.M., Kenner, G.S., Gould, T.R., (1983) J Dent Res, 62, pp. 1045-1048; 
Pelaez, A., Pareja, A., Garcia, C., Marquez, M., (2005) Key Eng Mater, 284, pp. 593-596; 
Curtis, A., Wilkinson, C., (1997) Biomaterials, 18, pp. 1573-1583; 
Ito, Y., (1999) Biomaterials, 20, pp. 2333-2342; 
Tan, J., Saltzman, W.M., (2004) Biomaterials, 25, pp. 3593-3601; 
Gallego, D., Ferrell, N., Sun, Y., Hansford, D.J., (2008) Mater Sci Eng C, 28, pp. 353-358; 
Xia, Y., Whitesides, G.M., (1998) Angew. Chem. Int. Ed, 37, pp. 550-575
},
	month = {2009///},
	pages = {303 - 306},
	abstract = {Cell behaviour such as adhesion, morphology, proliferation and functional activity are highly influenced by surface properties including hydrophobicity, roughness, texture and morphology. These surface properties may be controlled using a mixture of additive coating techniques to produce glass coatings by sol-gel process and soft lithography on dental ceramics. The purpose of this work was to compare cell adhesion and early orientation of Human Bone Marrow (HBM) cells cultured on micro-patterned (micro-PGC) and on flat glass coatings (FGC) produced by sol-gel processing. Spin coating was used to apply SiO                        2 flat coatings on glass substrates as model surfaces. Photolithography was applied to produce master patterns with microscale dimensions. A moulding technique was used to print micropatterned SiO 
                        2 glass coatings produced by a sol-gel process. The coatings were then sintered, sterilized and cultured with HBM cells derived from primary cultures, using a standardized protocol, for 1 and 7 days. Cell morphology and orientation were observed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Flat and MPGC with line shaped features were produced. Cells presented a typical osteoblastic morphology on flat surfaces while slimmer, preferentially oriented and more elongated morphologies could be seen on line micro-patterned surfaces. HBM cells cultured on flat glass coatings showed increased tendency to spread and to assume more randomized proliferation when compared to the cells on the micro-patterned glass coatings. Micro-patterned glass coatings showed higher orientation control and smaller delay in the rate of proliferation, in early stages of in vitro culture as compared to flat coatings. These preliminary studies revealed that Micro-PGC induce significant morphological changes and controlled orientation of HBM cells during early stages of cell proliferation.
},
	keywords = {Adhesion, cell adhesion, Cell behaviours, Cell culture, Cell morphologies, cell proliferation, Cells, Coatings, Colloids, Confocal laser scanning microscopies, confocal microscopy, Contact guidance, Controlled orientations, cytology, Dental ceramics, Dental Materials, Flat glass, Flat surfaces, Functional activities, Gelation, Gels, glass, Glass ceramics, Glass coatings, Glass substrates, In-vitro, Micro-patterned, Micro-patterned surfaces, Micro-scale, Micromachining, Micropatterning, Model surfaces, Morphological changes, morphology, Nanostructured materials, Orientation controls, Primary cultures, Scanning, Scanning electron microscopy, Scanning electrons, SEM, Silicon compounds, Soft lithographies, Sol-gel, Sol-gel process, Sol-gel processing, Sol-gels, Sols, Surface Properties, Thermal spraying, Trace analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849141765\&partnerID=40\&md5=7a4c975e741442308a8db69108047673},
	author = {Pelaez-Vargas, A. and Ferrell, N. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
