



@article {2276,
	title = {In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {93},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32532PubMed ID: 19591232Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomaterials, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: sandra_teixeira3@yahoo.comChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Biocompatible Materials; Ceramics; Durapatite, 1306-06-5References: Jones, A.C., Milthorpe, B., Averdunk, H., Limaye, A., Senden, T.J., Sakellariou, A., Sheppard, A.P., Dwdw, H., Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging (2004) Biomaterials, 25, pp. 4947-4954;Aizawa, M., Shinoda, H., Uchida, H., Itatani, K., Okada, I., Matsumoto, M., Toyama, Y., Development and biological evaluation of apatite fibre scaffolds with large pore size and high porosity for bone regeneration (2003) Bioceramics, 240-242, pp. 647-650; 
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},
	month = {2010///},
	pages = {567 - 575},
	abstract = {During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA based porous scaffolds with collagen type I. The results indicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds. {\textcopyright} 2009 Wiley Periodicals, Inc.},
	keywords = {animal cell, animal experiment, animal model, Animals, article, Biocompatibility, Biocompatible Materials, Biodegradation, Bone, Bone and Bones, Bone formation, bone tissue, Calcium, Calcium alloys, calcium phosphate, Calcium phosphates, Cell Count, Cell culture, Cell Culture Techniques, Cells, Cultured, cellular distribution, Ceramic materials, ceramics, Collagen, collagen type 1, Durapatite, Energy dispersive spectroscopy, Flowcharting, Fourier transform infrared spectroscopy, Humans, hydroxyapatite, Hydroxylation, in vitro study, In vivo bone formation, in vivo study, In-vivo, infrared spectroscopy, Macroporous, Macroporous scaffolds, male, materials testing, Mesenchymal stem cell, Mesenchymal Stem Cells, Mice, Microscopy, Electron, Scanning, nonhuman, ossification, Osteogenesis, porous polymer, rat, Rats, Rats, Wistar, Scaffolds, Scanning electron microscopy, Spectroscopy, Fourier Transform Infrared, Stem cells, Tissue engineering, tissue scaffold, Tissue Scaffolds},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77950190285\&partnerID=40\&md5=612e26b56020935c7b0b1c564bffa89a},
	author = {Teixeira, S. and Fernandes, H. and Leusink, A. and Van Blitterswijk, C. and Ferraz, M.P. and Monteiro, F.J. and De Boer, J.}
}
