



@article {2283,
	title = {Comparison of a computer system evaluation of intrapartum cardiotocographic events and a consensus of clinicians.},
	journal = {Journal of perinatal medicineJ Perinat Med},
	volume = {38},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1515/JPM.2010.030PubMed ID: 20121542Language of Original Document: EnglishCorrespondence Address: Costa, M.A.email: cosantonia@gmail.com},
	month = {2010///},
	pages = {191 - 195},
	abstract = {AIMS: To compare between computer analysis of intrapartum cardiotocography (CTG) features by the Omniview-SisPorto 3.5 and a consensus of clinicians. METHODS: Agreement study using 50 consecutively acquired tracings (206 h of signals) with >60 min duration, <10\% signal loss and recorded in labor at term by internal fetal heart rate (FHR) monitoring. Tracings were divided into 10-min segments and independently analyzed by three experienced clinicians, in order to estimate the FHR baseline and identify periodic events. A consensus was reached using a three round Delphi procedure. Results were compared with the analysis provided by the Omniview-SisPorto 3.5 system. RESULTS: For baseline estimation, agreement between the computer and the consensus was high [intraclass correlation coefficient (ICC)=0.85; 95\% confidence interval (CI) 0.46-0.93], with a mean difference of 3.7 bpm (limits of agreement -4.4-11.9 bpm), and 99\% of differences under 15 bpm. A concordant identification was observed in 71\% of accelerations (95\% CI: 69\%-73\%), 68\% of decelerations (95\% CI: 66\%-70\%), and 87\% of uterine contractions (95\% CI: 85\%-89\%). CONCLUSIONS: A high agreement was observed between the Omniview-SisPorto 3.5 and a consensus of clinicians in evaluation of intrapartum CTG baseline, accelerations, decelerations and uterine contractions.},
	keywords = {algorithm, Algorithms, article, Cardiotocography, comparative study, computer assisted diagnosis, Diagnosis, Computer-Assisted, Female, Fetal Heart, Fetal monitoring, fetus heart, fetus monitoring, human, Humans, instrumentation, methodology, physician, Physicians, physiology, Pregnancy},
	isbn = {16193997 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953038696\&partnerID=40\&md5=bca187de4ffea0b2f2c4d2dcfb2140d9},
	author = {Costa, M.A. and Ayres-de-Campos, D. and Machado, A.P. and Santos, C.C. and Bernardes, J.}
}
@article {2324,
	title = {Physical, chemical and in vitro biological profile of chitosan hybrid membrane as a function of organosiloxane concentration},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 10Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2008.07.022PubMed ID: 18722166Language of Original Document: EnglishCorrespondence Address: Hayakawa, S.; Biomaterial Laboratory, Graduate School of Natural Science and Technology, Okayama University, Tsushima, Okayama-shi, 700-8530, Japan; email: satoshi@cc.okayama-u.ac.jpChemicals/CAS: (3 glycidyloxypropyl)trimethoxysilane, 2530-83-8; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; chitosan, 9012-76-4; silicate, 12627-13-3; Chitosan, 9012-76-4; Cross-Linking Reagents; gamma-glycidoxypropyltrimethoxysilane, 2530-83-8; Silanes; SiloxanesTradenames: AldrichReferences: Lai, J.Y., Lin, F.C., Wang, C.C., Wang, D.M., Effect of nonsolvent additives on the porosity and morphology of asymmetric TPX membranes [J] (1996) J Membrane Sci, 118, pp. 49-61;Wang, D.M., Lin, F.C., Chiang, J.C., Lai, J.Y., Control of porosity of asymmetric TPX membranes [J] (1998) J Membrane Sci, 141, pp. 1-12; 
Piattelli, A., Scarano, A., Russo, P., Matarasso, S., Evaluation of guided bone regeneration in rabbit tibia using bioresorbable and non-resorbable membranes (1996) Biomaterials, 17 (8), pp. 791-796; 
Gotfredsen, K., Nimb, L., Hjorting-Hansen, E., Immediate implant placement using a biodegradable barrier, polyhydroxybutyratehydroxyvalerate reinforced with polyglactin 910: an experimental study in dogs (1994) Clin Oral Implants Res, 5 (2), pp. 83-91; 
Kostopoulos, L., Karring, T., Augmentation of the rat mandible using guided tissue regeneration (1994) Clin Oral Implants Res, 5 (2), pp. 75-82; 
Kostopoulos, L., Karring, T., Guided bone regeneration in mandibular defects in rats using a bioresorbable polymer (1994) Clin Oral Implants Res, 5 (2), pp. 666-674; 
Nyman, S., Gottlow, J., Karring, T., The regeneration potential of the periodontal ligament: an experimental study in the monkey (1982) J Clin Periodontal, 9, pp. 257-265; 
Dupoirieux, L., Pourquier, D., Picot, M.C., Neves, M., Comparative study of three different membranes for guided bone regeneration of rat cranial defects (2001) Int J Oral Maxillofac Surg, 30 (1), pp. 58-62; 
Zhang, R., Ma, P.X., Porous poly(l-lactic acid)/apatite composites created by biomimetic process (1999) J Biomed Mater Res, 45, pp. 285-293; 
Murphy, W.L., Kohn, D.H., Mooney, D.J., Growth of continuous bonelike mineral within porous poly(lactide-co-glycolide) scaffolds in vitro (2000) J Biomed Mater Res, 50, pp. 50-58; 
Lin, F.C., Wang, D.M., Lai, J.Y., Asymmetric TPX membranes. J membranes with high gas flux. (1996) J Membrane Sci, 110, pp. 25-36; 
Vert, M., Mauduit, J., Li, S., Biodegradation of PLA/GA polymers: increasing complexity (1994) Biomaterials, 15, pp. 1209-1213; 
Lee, Y.K., Mooney, D.J., Hydrogels for tissue enigineering (2001) Chem Rev, 101, pp. 1869-1879; 
Domard, A., Rinaudo, M., Preparation and characterization fully deacetylated chitosan (1983) Int J Biol Macromol, 5, pp. 49-52; 
Chandy, T., Sharma, P.C., Chitosan - as a biomaterial (1990) Biomater Artif Cells Artif Organo, 18, pp. 1-24; 
Ueno, H., Yamada, H., Tanaka, I., Kaba, N., Matsuura, M., Okumura, M., Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs (1999) Biomaterials, 20, pp. 1407-1414; 
Muzzareli, R.A.A., Mattioli-Belmonte, M., Tietz, C., Biagini, R., Ferioli, G., Brunelli, M.A., Stimulatory effect on bone formation exerted by a modified chitosan (1994) Biomaterials, 15, pp. 1075-1081; 
Shirosaki, Y., Tsuru, K., Hayakawa, S., Osaka, A., Lopes, M.A., Santos, J.D., In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes (2005) Biomaterials, 26, pp. 485-493; 
Prochazkova, S., Varum, K.M., {\AE}stgaard. Quantitative determination of chitosans by ninhydrin (1999) Carbohydr Polym, 38, pp. 115-122; 
Coelho, M.J., Fernandes, M.H., Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation (2000) Biomaterials, 21, pp. 1095-1102; 
Coelho, M.J., Cabral, A.T., Fernandes, M.H., Human bone cell cultures in biocompatibility testing. Part I: osteoblastic differentiation of serially passaged human bone marrow cells cultured in α-MEM and in D-MEM (2000) Biomaterials, 21, pp. 1087-1094; 
Berridge, V., Tan, A.S., Characterization of the cellular reduction of 3-(4,5-dimethhylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction (1993) Arch Biochem Biophys, 303, pp. 474-482; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arg{\"u}elles-Monal, W., Desbri{\'e}res, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Ren, L., Tsuru, K., Hayakawa, S., Osaka, A., Sol-gel preparation and in vitro deposition of apatite on porous gelatin-siloxane hybrids (2001) J Non Cryst Solid, 285, pp. 116-122; 
Ren, L., Tsuru, K., Hayakawa, S., Osaka, A., Novel approach to fabricate porous gelatin-siloxane hybrids for bone tissue engineering (2002) Biomaterials, 23, pp. 4765-4773; 
Ren, L., Tsuru, K., Hayakawa, S., Osaka, A., Incorporation of Ca 2+ ions in gelatin-siloxane hybrids through a sol-gel process (2001) J Ceram Soc Jpn, 109, pp. 403-408; 
Rashidova, S.S.h., Shakarova, D.S.h., Ruzimuradov, O.N., Satabaldieva, D.T., Zalyalieva, S.V., Shpigun, O.A., Bionanocompositional chitosan-silica sorbent for liquid chromatography (2004) J Chromatogr B, 800, pp. 49-53; 
Hench, L.L., Ethridge, E.C., (1982) Biomaterials an interfacial approach, , Academic Press, New York 10; 
Xynos, I.D., Hukkanen, M.V.J., Batten, J.J., Buttery, L.D., Hench, L.L., Polak, J.M., Bioglass {\textregistered} 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering (2000) Calcif Tissue Int, 67, pp. 321-329; 
Xynos, I.D., Edgar, A.J., Buttery, L.D.K., Hench, L.L., Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis (2000) Biochem Biophys Res Commun, 276, pp. 461-465; 
Gough, J.E., Jones, J.R., Hench, L.L., Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold (2004) Biomaterials, 25, pp. 2039-2046; 
Maniatopoulos, C., Sodek, J., Melcher, A., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Fernandes, M.H., Costa, M.A., Carvalho, G.S., Mineralization in serially passaged human alveolar bone cells (1997) J Mater Sci Mater Med, 8, pp. 61-65; 
Costa, M.A., Gutierres, M., Almeida, L., Lopes, M.A., Santos, J.D., Fernandes, M.H., In vitro mineralization of human bone marrow cells cultured on Bonelike {\textregistered} (2004) Key Eng Mater, 254-256, pp. 821-824
},
	month = {2009///},
	pages = {346 - 355},
	abstract = {We attempted to prepare chitosan-silicate hybrid for use in a medical application and evaluated the physico-chemical properties and osteocompatibility of the hybrids as a function of γ-glycidoxypropyltrimethoxysilane (GPTMS) concentration. Chitosan-silicate hybrids were synthesized using GPTMS as the reagent for cross-linking of the chitosan chains. Fourier transform infrared spectroscopy,                        29Si CP-MAS NMR spectroscopy and the ninhydrin assay were used to analyze the structures of the hybrids, and stress-strain curves were recorded to estimate their Young{\textquoteright}s modulus. The swelling ability, contact angle and cytocompatibility of the hybrids were investigated as a function of the GPTMS concentration. A certain fraction of GPTMS in each hybrid was linked at the epoxy group to the amino group of chitosan, which was associated with the change in the methoxysilane group of GPTMS due to hybridization. The cross-linking density was around 80\% regardless of the volume of GPTMS. As the content of GPTMS increased, the water uptake decreased and the hydrophilicity of the hybrids increased except when the content exceeded amolar ratio of 1.5, when it caused a decrease. The values of the mechanical parameters assessed indicated that significant stiffening of the hybrids was obtained by the addition of GPTMS. The adhesion and proliferation of the MG63 osteoblast cells cultured on the chitosan-GPTMS hybrid surface were improved compared to those on the chitosan membrane, regardless of the GPTMS concentration. Moreover, human bone marrow osteoblast cells proliferated on the chitosan-GPTMS hybrid surface and formed a fibrillar extracellular matrix with numerous calcium phosphate globular structures, both in the presence and in the absence of dexamethasone. Therefore, the chitosan-GPTMS hybrids are promising candidates for basic materials that can promote bone regeneration because of their controllable composition (chitosan/GPTMS ratio). {\textcopyright} 2008 Acta Materialia Inc.
},
	keywords = {(3 glycidyloxypropyl)trimethoxysilane, article, assay, Biocompatibility, Bone Marrow Cells, calcium phosphate, cell adhesion, Cell Line, Tumor, cell proliferation, cell survival, cell viability, Chemical analysis, chitosan, concentration (parameters), controlled study, cross linking, Cross-Linking Reagents, extracellular matrix, human, human cell, Humans, hydrophobicity, in vitro study, infrared spectroscopy, Magnetic Resonance Spectroscopy, Microscopy, Electron, Scanning, molecular hybridization, Nuclear magnetic resonance spectroscopy, Organic-inorganic hybrid, osteoblast, Osteoblasts, Osteocompatibility, physical chemistry, priority journal, Silanes, silicate, siloxane, Siloxanes, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, stress strain relationship, structure analysis, surface property, Tissue engineering, unclassified drug, water transport, γ-Glycidoxypropyltrimethoxysilane (GPTMS)},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-56649083638\&partnerID=40\&md5=550d9a49b04985f38609e86d9adc5735},
	author = {Shirosaki, Y. and Tsuru, K. and Hayakawa, S. and Osaka, A. and Lopes, M.A. and Santos, J.D. and Costa, M.A. and Fernandes, M.H.}
}
@book {2488,
	title = {Biological Activity of Two Glass Ceramics in the Meta- and Pyrophosphate Region: A Comparative Study},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Dias, A.G.; Faculdade de Engenharia, Universidade do Porto, DEMM, Rua Dr. Roberta Frias, 4200 Porto, PortugalReferences: Dias, A.G., Lopes, M.A., Gibson, I., Santos, J.D., J. Non-Crystal. Solids, , in press;Dias, A.G., Lopes, M.A., Tsuru, K., Hayakawa, S., Santos, J.D., Osaka, A., Physiscs and Chemistry of Glasses, , in press; 
Dias, A.G., Lopes, M.A., Santos, J.D., Advance Materials Science, , in press; 
Rykke, M., Rolla, G., Sonju, T., (1988) Scand. J. Dent. Res., 96, pp. 517-522
},
	month = {2004///},
	pages = {825 - 828},
	address = {Porto},
	abstract = {Based upon the CaO-P                        2O
                        5 glass system, two glass ceramics were prepared in the metaand pyrophosphate regions. The present work describes preliminary results concerning the biological activity of MK5B (45CaO-45P
                        2O
                        5-5MgO-5K20, in mol \%) and MT13B (45CaO-37P
                        2O
                        55-5MgO-13TiO
                        2, in mol \%) using MG63 osteoblast-like cells. Both materials supported cell growth and proliferation which increased with the incubation time. However, cell growth was significantly lower on seeded MK5B samples. SEM observation showed that cell adhesion and spreading were hampered on this material. Evident degradation of the material surface was observed with simultaneous cell proliferation and material precipitation. By contrast, MT13B presented a relatively stable surface throughout the culture time. Results suggest that the in vitro biological behaviour of MK5B and MT13B is related with differences in the degradation rates of the two materials during the culture time.
},
	keywords = {Adhesion, Biodegradable glass ceramics, Biodegradation, Bone, cell proliferation, Cells, Crystallization, Degradation, Glass ceramics, incubation time, MG63 osteoblast-like cells, Scanning electron microscopy, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0348146001\&partnerID=40\&md5=5245524d4dfb5615946aadcf5eb7c37a},
	author = {Dias, A.G. and Costa, M.A. and Lopes, M.A. and Santos, J.D. and Fernandas, M.H.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2499,
	title = {In Vitro Mineralisation of Human Bone Marrow Cells Cultured on Bonelike{\textregistered}},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Costa, M.A.; Inst. Cie. Blomed. de Abel Salazar, Universidade do Porto, Largo Abel Salazar, 4000 Porto, PortugalReferences: Santos, J.D., Campbell, J.K., Winston, G.H., European Patent N{\textdegree} WO0068164, 2000Lopes, M.A., Knowles, J.C., Santos, J.D., (2000) Biomaterials, 21, p. 1905;Lopes, M.A., Monteiro, F.J., Santos, J.D., (1999) J. Biomed. Mater. Res.:App. Biomater., 48, p. 734; 
Lopes, M.A., Knowles, J.C., Kuru, L., Santos, J.D., Monteiro, F.J., Olsen, I., (1998) J. Biomed. Mater. Res., 41, p. 649; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., (2000) Biomaterials, 21, p. 1165; 
Ferraz, M.P., Fernandes, M., Santos, J.D., Monteiro, F.J., (2001) J. Mat. Sci.: Mat. Med., 12, p. 629; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., (2001) J. Biomed. Mater. Res., 54, p. 463; 
Duarte, F., Santos, J.D., Afonso, A., Advanced Materials Forum, , in press; 
Coelho, M.J., Fernandes, M.H., (2000) Biomaterials, 21, p. 1095; 
Kleerekoper, M., (1996) Principles of Bone Biology, p. 1053. , Academic Press
},
	month = {2004///},
	pages = {821 - 824},
	address = {Porto},
	abstract = {Bonelike{\textregistered} is a CaO-P                        2O
                        5 based glass-reinforced hydroxyapatite (HA) designed to mimic the inorganic composition of the bone tissue. This work evaluates the response of human bone marrow cells to Bonelike{\textregistered} concerning cell proliferation and osteoblast differentiation. HA was used as control material. Results showed that Bonelike{\textregistered} allowed the proliferation of bone marrow cells and their complete differentiation, as evidenced by the formation of cell-mediated mineralisation. In comparison with HA, Bonelike{\textregistered} had a positive effect on the expression of alkaline phosphatase and also on the formation of a mineralised matrix, two osteoblast markers.
},
	keywords = {Bone, Bonelike{\textregistered}, Cell culture, cell proliferation, Composition, Grafts, Human bone marrow cells, hydroxyapatite, In vitro mineralisation, mineralization, Scanning electron microscopy, Sintering, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346255201\&partnerID=40\&md5=470ac94fd75d1a53b887d44f59fbf7d1},
	author = {Costa, M.A. and Gutierres, M. and Almeida, L. and Lopes, M.A. and Santos, J.D. and Fernandes, M.H.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2524,
	title = {Si                    3N
                    4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells
},
	journal = {BiomaterialsBiomaterials},
	volume = {23},
	year = {2002},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(02)00249-1PubMed ID: 12361631Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Universidade do Porto, Rua Dr Manuel Pereira da Silva, 4200-393 Porto, Portugal; email: mhrf@portugalmail.ptChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; Calcium, 7440-70-2; Phosphorus, 7723-14-0; Silicon Compounds; silicon nitride, 12033-89-5References: Amaral, M., Lopes, M.A., Silva, R.F., Santos, J.D., Densification route and mechanical properties of Si 3N 4-bioglass biocomposites (2002) Biomaterials, 23, pp. 857-862;Kue, R., Sahrabi, A., Nagle, D., Frondonza, C., Hungerford, D., Enhanced proliferation and osteocalcin production by human osteoblast-like cells on silicon nitride ceramic discs (1999) Biomaterials, 20, pp. 1195-1201; 
Howlett, C., McCartney, E., Ching, W., The effects of silicon nitride ceramic on rabbit skeletal cells and tissues (1989) Clin Orthop, 244, pp. 296-301; 
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Costa, M.A., Fernandes, M.H., Long-term effects of parathyroid hormone, 1,25-dihydroxyvitamin D3 and dexamethasone on the cell growth and functional activity of human osteogenic alveolar bone cell cultures (2000) Pharmacol Res, 42, pp. 345-353; 
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De Santis, D., Guerriero, C., Nocini, P.F., Vangersbock, A., Richard, G., Gotte, P., Armato, U., Adult human bone cells from jaw bones cultured on plasma-sprayed or polished surfaces of titanium or hydroxyapatite discs (1996) J Mater Sci Mater Med, 7, pp. 21-28; 
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Ferraz, M.P., Fernandes, M.H., Trigo Cabral, A., Santos, J.D., Monteiro, F.J., In vitro growth and differentiation of osteoblastic-like human bone marrow cells on a glass reinforced hydroxyapatite plasma-speayed coating (1999) J Mater Sci: Mater Med, 10, pp. 567-576; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of the effects of pre-immersion on the biocompatibility of glass-reinforced hydroxyapatite plasma sprayed coatings (2000) Biomaterials, 21, pp. 813-820; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., HA and double-layer HA/CaO-P 2O 5 glass coatings: Influence of chemical composition on human bone marrow cells osteoblastic behaviour (2001) J Mater Sci: Mater Med, 7, pp. 628-638; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Direct and indirect effects of P 2O 5-glass reinforced hydroxyapatite composites on the growth and function of osteoblast-like cells (2000) Biomaterials, 21, pp. 1165-1172; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J Biomed Mater Res, 54, pp. 463-469; 
Gwynn, I.A.P., Cell biology at interfaces (1994) J Mater Sci Mater Med, 5, pp. 357-360; 
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},
	month = {2002///},
	pages = {4897 - 4906},
	abstract = {The in vitro osteocompatibility of a novel Si                        3N
                        4-bioglass composite (70-30\% weight proportion) with improved mechanical properties (fracture toughness=4.4MPam
                        1/2; bending strength=383{\textpm}47MPa) is reported. Immersion of the composite samples in culture medium (30min to 7 days) resulted in rapid protein adsorption to the surface and, also, dissolution of the intergranular phase of bioglass (time-dependent process) with the formation of different size cavities. "As-received" and pre-treated material samples presented a similar behaviour concerning the proliferation of MG63 osteoblast-like cells, evaluated during a 5-day culture period. Seeded materials showed a higher cell growth rate as compared to cultures performed on the standard plastic culture plates. To assess the osteogenic potential of the composite, "as-received" material samples were seeded with human bone marrow cells and cultured for 35 days in experimental conditions that favour the development of the osteoblastic phenotype. The cell adhesion process was similar to that observed in control cultures. Cells successfully adapted to the irregularities of the surface and were able to grow towards inside the cavities; in addition, osteogenic differentiation occurred with the formation of abundant cell-mediated mineralised deposits. Results suggest that this Si
                        3N
                        4-bioglass composite seems to be a promising candidate for high-stress medical applications. {\textcopyright} 2002 Elsevier Science Ltd. All rights reserved.
},
	keywords = {3N, 4-bioglass composite, Adhesion, Adsorption, alkaline phosphatase, article, Biocompatibility, Bioglass, Biomaterials, bone marrow cell, Bone Marrow Cells, Calcium, cell adhesion, Cell Culture Techniques, cell differentiation, Cell Division, cell growth, cell proliferation, composite material, controlled study, culture medium, Dissolution, glass, Growth kinetics, human, Human bone marrow cells, human cell, Humans, immersion, in vitro study, Mechanical properties, MG63 osteoblast-like cells, Microscopy, Electron, Scanning, osteoblast, Osteoblasts, Osteocompatibility, Osteogenic differentiation, phenotype, phosphorus, priority journal, Proteins, rigidity, Si, Silicon compounds, silicon derivative, surface property, tensile strength, Time Factors},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036888731\&partnerID=40\&md5=2235d0ad5a92fb1ae088f736b6e7ca4d},
	author = {Amaral, M. and Costa, M.A. and Lopes, M.A. and Silva, R.F. and Santos, J.D. and Fernandes, M.H.}
}
@article {2609,
	title = {Mineralization in serially passaged human alveolar bone cells},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {8},
	year = {1997},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1018598430983Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Faculdade de Medicina Dentaria, Rua do Doutor Roberto Frias, 4200 Porto, PortugalReferences: Spector, M., Cease, C., Tong, X., (1989) Crit. Rev. Biocompat., 5, p. 269;Van Blitterswijk, C.A., Bakker, D., Hesseling, S.C., Koerten, H.K., (1991) Biomaterials, 12, p. 187; 
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},
	month = {1997///},
	pages = {61 - 65},
	publisher = {Chapman \& Hall Ltd},
	abstract = {Well-characterized human bone cell cultures have been regarded as a useful tool to study bone control mechanisms and also to analyse bone/biomaterials interactions. In the present study, human alveolar bone cells were cultured in a-minimal essential medium (α-MEM) containing 10\% foetal bovine serum (FBS), 50 μg/ml ascorbic acid, 10 mM sodium β-glycerophosphate and either in the presence or in the absence of 10 nM dexamethasone (Dexa). Cultures were characterized concerning cell viability/proliferation, alkaline phosphatase (ALP), acid phosphatase (ACP) and tartraric acid resistant phosphatase (TRAP) activities, and formation of mineralized areas. Cell proliferation increased gradually for approximately 20 days. In the presence of Dexa, cells formed isolated or interconnected multilayered clusters that increased with culture time. Histochemical assays revealed strong positive reactions for ALP and calcium and phosphates deposits, mainly in relation to cells associated with the clusters. High levels of ALP activity (biochemical determination) were observed. Cells cultured in the absence of Dexa showed significantly lower ALP activity and no calcium and phosphates deposits were present. Serially passaged cells kept the proliferation rate constant but a decrease in ALP activity was observed either in the presence or in the absence of Dexa. The ability to form mineralized areas (cultures fed with Dexa) also decreased on serial subculture.Well-characterized human bone cell cultures have been regarded as a useful tool to study bone control mechanisms and also to analyse bone/biomaterials interactions. In the present study, human alveolar bone cells were cultured in α-minimal essential medium (α-MEM) containing 10\% foetal bovine serum (FBS), 50 μg/ml ascorbic acid, 10 mM sodium β-glycerophosphate and either in the presence or in the absence of 10 nM dexamethasone (Dexa). Cultures were characterized concerning cell viability/proliferation, alkaline phosphatase (ALP), acid phosphatase (ACP) and tartaric acid resistant phosphatase (TRAP) activities, and formation of mineralized areas. Cell proliferation increased gradually for approximately 20 days. In the presence of Dexa, cells formed isolated or interconnected multilayered clusters that increased with culture time. Histochemical assays revealed strong positive reactions for ALP and calcium and phosphates deposits, mainly in relation to cells associated with the clusters. High levels of ALP activity (biochemical determination) were observed. Cells cultured in the absence of Dexa showed significantly lower ALP activity and no calcium and phosphates deposits were present. Serially passaged cells kept the proliferation rate constant but a decrease in ALP activity was observed either in the presence or in the absence of Dexa. The ability to form mineralized areas (cultures fed with Dexa) also decreased on serial subculture.
},
	keywords = {Acid phosphatase, Acid resistance, alkaline phosphatase, alveolar bone, article, ascorbic acid, Bioassay, Biocompatibility, Biomaterials, Bone, bone cell, Bovinae, Cell culture, cell proliferation, Characterization, Chemical reactions, controlled study, culture medium, dexamethasone, Foetal bovine serum, glycerophosphate, Histochemical assays, histochemistry, human, Human alveolar bone cells, human cell, mineralization, Minimal essential medium, Phosphates, priority journal, Proteins, tartaric acid, Tartraric acid resistant phosphatase, Tissue culture},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031081528\&partnerID=40\&md5=2c2c8ad80457e512ebcefac13930c670},
	author = {Fernandes, M.H. and Costa, M.A. and Carvalho, G.S.}
}
