



@article {2287,
	title = {Anti-adhesion and antiproliferative cellulose triacetate membrane for prevention of biomaterial-centred infections associated with Staphylococcus epidermidis},
	journal = {International Journal of Antimicrobial AgentsInt. J. Antimicrob. Agents},
	volume = {35},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: IAAGEdoi: 10.1016/j.ijantimicag.2009.09.017PubMed ID: 19942411Language of Original Document: EnglishCorrespondence Address: Extremina, C.I.; REQUIMTE, Faculdade de Farm{\'a}cia, Universidade do Porto, Rua An{\'\i}bal Cunha 164, 4050-047 Porto, Portugal; email: extremina@gmail.comChemicals/CAS: cellulose triacetate, 9012-09-3; cilastatin plus imipenem, 92309-29-0; Anti-Bacterial Agents; Biocompatible Materials; Cellulose, 9004-34-6; Imipenem, 74431-23-5; cellulose triacetate, 9012-09-3Tradenames: tienam, Merck Sharp and Dohme, GermanyManufacturers: Eastman Kodak, Netherlands; Merck Sharp and Dohme, GermanyReferences: Darouiche, R.O., Treatment of infections associated with surgical implants (2004) N Engl J Med, 350, pp. 1422-1429;Fonseca, A.P., Extremina, C., Fonseca, A.F., Sousa, J.C., Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa (2004) J Med Microbiol, 53, pp. 903-910; 
Donelli, G., Francolini, I., Piozzi, A., Di Rosa, R., Marconi, W., New polymer-antibiotic systems to inhibit bacterial biofilm formation: a suitable approach to prevent central venous catheter-associated infections (2002) J Chemother, 14, pp. 501-507; 
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Mart{\'\i}nez-Mart{\'\i}nez, L., Pascual, A., Giglio, M.I., Perea, E.J., Effect of subinhibitory concentrations of β-lactams on the production of slime, surface hydrophobicity and adhesion of Staphylococcus epidermidis [in Spanish] (1991) Enferm Infecc Microbiol Clin, 9, pp. 543-546
},
	month = {2010///},
	pages = {164 - 168},
	abstract = {The initial step in preventing biomaterial-associated infections consists of preventing bacterial adhesion to the device surface. One possible approach is the design of antibiotic-releasing biomaterials. Cellulose triacetate (CTA) membranes with the antibiotic imipenem (IPM) entrapped (CTA-IPM) were prepared. The material was characterised in terms of surface morphology by scanning electron microscopy, surface free energy of interaction and X-ray photoelectron spectroscopy (XPS). Antibiotic release studies were also performed. In vitro adhesion of Staphylococcus epidermidis RP62A to CTA-IPM was investigated using a modified microtitre plate assay, and the antibacterial activity of the CTA-IPM membrane was assessed by a modified Kirby-Bauer test, which showed effective entrapment of the antibiotic as confirmed by XPS and hydrophilicity assays. Release studies showed that this drug-polymer conjugate serves as an adequate reservoir for sustained release of IPM over a period of 71 h at an effective bacteriostatic concentration. Moreover, bacterial adhesion tests showed a statistically significant decrease in the adhesion of S. epidermidis RP62A to CTA-IPM compared with its adhesion to CTA alone. The present innovative approach is capable of providing a membrane with anti-adhesive and antiproliferative properties, thus encouraging in vivo studies to provide a better simulation of the clinical situation. {\textcopyright} 2009 Elsevier B.V. and the International Society of Chemotherapy.},
	keywords = {Adhesion, Anti-Bacterial Agents, antibacterial activity, Antimicrobial agents, article, Bacterial adhesion, bacterial growth, bacteriostasis, bacterium adherence, Biocompatible Materials, biomaterial, Cellulose, Cellulose triacetate, cilastatin plus imipenem, controlled study, device infection, drug screening, Equipment and Supplies, growth inhibition, Humans, hydrophilicity, Imipenem, in vitro study, infection prevention, Membranes, Microbial Sensitivity Tests, microtiter plate assay, nonhuman, priority journal, Prosthesis-Related Infections, Scanning electron microscopy, Staphylococcus epidermidis, surface property, sustained drug release, X ray photoelectron spectroscopy},
	isbn = {09248579 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-72649093658\&partnerID=40\&md5=3fbe3d7e15447e7ae40bb5fdd3f63b61},
	author = {Extremina, C.I. and Fonseca, A.F.d. and Granja, P.L. and Fonseca, A.P.}
}
