



@article {2287,
	title = {Anti-adhesion and antiproliferative cellulose triacetate membrane for prevention of biomaterial-centred infections associated with Staphylococcus epidermidis},
	journal = {International Journal of Antimicrobial AgentsInt. J. Antimicrob. Agents},
	volume = {35},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: IAAGEdoi: 10.1016/j.ijantimicag.2009.09.017PubMed ID: 19942411Language of Original Document: EnglishCorrespondence Address: Extremina, C.I.; REQUIMTE, Faculdade de Farm{\'a}cia, Universidade do Porto, Rua An{\'\i}bal Cunha 164, 4050-047 Porto, Portugal; email: extremina@gmail.comChemicals/CAS: cellulose triacetate, 9012-09-3; cilastatin plus imipenem, 92309-29-0; Anti-Bacterial Agents; Biocompatible Materials; Cellulose, 9004-34-6; Imipenem, 74431-23-5; cellulose triacetate, 9012-09-3Tradenames: tienam, Merck Sharp and Dohme, GermanyManufacturers: Eastman Kodak, Netherlands; Merck Sharp and Dohme, GermanyReferences: Darouiche, R.O., Treatment of infections associated with surgical implants (2004) N Engl J Med, 350, pp. 1422-1429;Fonseca, A.P., Extremina, C., Fonseca, A.F., Sousa, J.C., Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa (2004) J Med Microbiol, 53, pp. 903-910; 
Donelli, G., Francolini, I., Piozzi, A., Di Rosa, R., Marconi, W., New polymer-antibiotic systems to inhibit bacterial biofilm formation: a suitable approach to prevent central venous catheter-associated infections (2002) J Chemother, 14, pp. 501-507; 
Akiyama, H., Okamoto, S., Prophylaxis of indwelling urethral catheter infection: clinical experience with a modified Foley catheter and drainage system (1979) J Urol, 121, pp. 40-42; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., Cellulose-based biomaterials for bone regeneration: hemi-synthesis and characterisation (1996) Proceedings of polymers in medicine and surgery-PIMS{\textquoteright}96, pp. 73-80. , Glasgow, UK; 
Elam, J.H., Karlsson, C.H., Nygren, H., Pre-adsorption of cellulose ether onto polymer surfaces: adsorption of adhesins and platelet activation (1992) Biomaterials, 14, pp. 233-237; 
Fonseca, A.P., Granja, P.L., Nogueira, J.A., Oliveira, D.R., Barbosa, M.A., Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives (2001) J Mater Sci Mater Med, 12, pp. 543-548; 
Performance standards for antimicrobial susceptibility testing (2006) Sixteenth informational supplement. Document M100-S16. Wayne, PA: CLSI, , Clinical and Laboratory Standards Institute; 
Vacheethasanee, K., Temenoff, J.S., Higashi, J.M., Gary, A., Anderson, J.M., Bayston, R., Bacterial surface properties of clinically isolated Staphylococcus epidermidis strains determine adhesion on polyethylene (1998) J Biomed Mater Res, 42, pp. 425-432; 
Bhat, N.V., Wavhal, D.S., Preparation of cellulose triacetate pervaporation membrane by ammonia plasma treatment (2000) J Appl Polym Sci, 76, pp. 258-265; 
Amaral, I.F., Granja, P.L., Melo Luis, V., Saramago, B., Barbosa, M.A., Functionalization of chitosan membranes through phosphorylation: atomic force microscopy, wettability, and cytotoxicity studies (2006) J Appl Polym Sci, 102, pp. 276-284; 
Thomas, M.J.K., Ando, D.J., (1996) Ultraviolet and visible spectroscopy: analytical chemistry by open learning, , Wiley, New York, NY; 
(1992) High resolution XPS of organic polymers: the Scienta ESCA300 database, , Beamson G., and Briggs D. (Eds), John Wiley \& Sons Ltd, Chichester, UK; 
Yigong Ge, Wikler, M.A., Sahm, D.F., Blosser-Middleton, R.S., Karlowsky, J.A., In vitro antimicrobial activity of doripenem, a new carbapenem (2004) Antimicrob Agents Chemother, 48, pp. 1384-1396; 
Mart{\'\i}nez-Mart{\'\i}nez, L., Pascual, A., Giglio, M.I., Perea, E.J., Effect of subinhibitory concentrations of β-lactams on the production of slime, surface hydrophobicity and adhesion of Staphylococcus epidermidis [in Spanish] (1991) Enferm Infecc Microbiol Clin, 9, pp. 543-546
},
	month = {2010///},
	pages = {164 - 168},
	abstract = {The initial step in preventing biomaterial-associated infections consists of preventing bacterial adhesion to the device surface. One possible approach is the design of antibiotic-releasing biomaterials. Cellulose triacetate (CTA) membranes with the antibiotic imipenem (IPM) entrapped (CTA-IPM) were prepared. The material was characterised in terms of surface morphology by scanning electron microscopy, surface free energy of interaction and X-ray photoelectron spectroscopy (XPS). Antibiotic release studies were also performed. In vitro adhesion of Staphylococcus epidermidis RP62A to CTA-IPM was investigated using a modified microtitre plate assay, and the antibacterial activity of the CTA-IPM membrane was assessed by a modified Kirby-Bauer test, which showed effective entrapment of the antibiotic as confirmed by XPS and hydrophilicity assays. Release studies showed that this drug-polymer conjugate serves as an adequate reservoir for sustained release of IPM over a period of 71 h at an effective bacteriostatic concentration. Moreover, bacterial adhesion tests showed a statistically significant decrease in the adhesion of S. epidermidis RP62A to CTA-IPM compared with its adhesion to CTA alone. The present innovative approach is capable of providing a membrane with anti-adhesive and antiproliferative properties, thus encouraging in vivo studies to provide a better simulation of the clinical situation. {\textcopyright} 2009 Elsevier B.V. and the International Society of Chemotherapy.},
	keywords = {Adhesion, Anti-Bacterial Agents, antibacterial activity, Antimicrobial agents, article, Bacterial adhesion, bacterial growth, bacteriostasis, bacterium adherence, Biocompatible Materials, biomaterial, Cellulose, Cellulose triacetate, cilastatin plus imipenem, controlled study, device infection, drug screening, Equipment and Supplies, growth inhibition, Humans, hydrophilicity, Imipenem, in vitro study, infection prevention, Membranes, Microbial Sensitivity Tests, microtiter plate assay, nonhuman, priority journal, Prosthesis-Related Infections, Scanning electron microscopy, Staphylococcus epidermidis, surface property, sustained drug release, X ray photoelectron spectroscopy},
	isbn = {09248579 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-72649093658\&partnerID=40\&md5=3fbe3d7e15447e7ae40bb5fdd3f63b61},
	author = {Extremina, C.I. and Fonseca, A.F.d. and Granja, P.L. and Fonseca, A.P.}
}
@article {2334,
	title = {Molecular epidemiology of pseudomonas aeruginosa clinical isolates from Portuguese Central Hospital},
	journal = {Folia MicrobiologicaFolia Microbiol.},
	volume = {53},
	year = {2008},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: FOMIAdoi: 10.1007/s12223-008-0086-yLanguage of Original Document: EnglishCorrespondence Address: Fonseca, A. P.Rua Roberto Frias s/n, 4200-465 Porto, Portugal; email: apfonseca08@sapo.ptChemicals/CAS: DNA, BacterialReferences: Bergmans, D., Bonten, M., van tiel, F., Gaillard, C., London, N., van der Geest, S., de Leeuw, P., Stobbrringh, E., Value of phenotyping methods as an initial screening of Pseudomonas aeruginosa in epidemiologic studies (1997) Infection, 25, pp. 350-354;Bertrand, X., Thouverez, M., Talon, D., Boillot, A., Capellier, G., Floriot, C., Helias, J.P., Endemicity, molecular diversity and colonization routes of Pseudomonas aeruginosa in intensive care units (2001) Intens.Care Med., 27, pp. 1263-1268; 
Br{\'\i}zio, A., Silva, A., Canas, E., Ferreira, T., Lito, L.M., Cristino, J.M., Salgado, M.J., Duarte, A., Spread of ln58 containing bla VIM-2 among Pseudomonas aeruginosa (2006) Clin.Microbiol.Infect.Dis., 12 (SUPPL. 4), p. 931; 
Br{\'\i}zio, A., Vasco, S., Gon{\c c}alves, A.R., Lito, L.M., Cristino, J.M., Salgado, M.J., Duarte, A., Survey of extended-spectrum β-lactamases in Escherichia coli isolates from a Portuguese hospital and characterisation of a novel class 1 integron (In60A) carrying the blaCTX-M-9 gene (2006) Internat.J.Antimicrob.Agents, 28, pp. 320-324; 
Buchholz, P., Richter, M., Fiedler, G., Halle, E., Epidemiological fingerprinting of multiresistant strains of Pseudomonas aeruginosa isolated from patients of intensive care units of the Charit{\'e} Hospital, Berlin (1995) Med.Microbiol.Lett., 4, pp. 7-13; 
{\v C}ernohorsk{\'a}, L., Votava, M., Antibiotic synergy against biofilm-forming Pseudomonas aeruginosa (2008) Folia Microbiol, 53, pp. 57-61; 
Elaichouni, A., Verschraegen, G., Claeys, G., Devleeschouwer, M., Godard, C., Vaneechoutte, M., Pseudomonas aeruginosa serotype O12 outbreak studied by arbitrary primer PCR (1994) J.Clin.Microbiol., 32, pp. 666-671; 
Fonseca, A.P., Correia, P., Fonseca, A.F., Tenreiro, R., Sousa, J.C., Polyphasic approach to the characterization of Pseudomonas aeruginosa isolates from a Portuguese Central Hospital (2004) Clin.Microbiol.Infect., 10 (SUPPL. 3), p. 1113; 
Fonseca, A.P., Correia, P., Sousa, J.C., Tenreiro, R., Association patterns of Pseudomonas aeruginosa clinical isolates as revealed by virulence traits, antibiotic resistance, serotype and genotype (2007) FEMS Immunol.Med.Microbiol., 51, pp. 505-516; 
Grundmann, H., Kropec, A., Hartung, D., Berner, R., Daschner, F., Pseudomonas aeruginosa in a neonatal intensive care unit: Reservoirs and ecology of the nosocomial pathogen (1993) J.Infect.Dis., 168, pp. 943-947; 
Grundmann, H.J., Towner, K.J., Dijkshoorn, L., Gerner-smidt, P., Maher, M., Seifert, H., Vaneechoutte, M., Multicenter study using standardized protocols and reagents for evaluation of reproducibility of PCR-based fingerprinting of Acinetobacter spp (1997) J.Clin.Microbiol., 35, pp. 3071-3077; 
Ho{\v s}tack{\'a}, A., {\v C}i{\v z}n{\'a}r, I., Slobodn{\'\i}kov{\'a}, L., Kotulov{\'a}, D., Clinical Pseudomonas aeruginosa: Potential factors of pathogenicity and resistance to antimicrobials (2006) Folia Microbiol, 51, pp. 633-638; 
Hunter, P.R., Gaston, M.M., Numerical index of the discriminatory ability of typing systems: An application of Simpson{\textquoteright}s index of diversity (1989) J.Clin.Microbiol., 26, pp. 2465-2466; 
Huston, W.M., Potter, A.J., Jennings, M.P., Rello, J., Hauser, A.R., McEwan, A.G., Survey of ferroxidase expression and siderophore production in clinical isolates of Pseudomonas aeruginosa (2004) J.Clin.Microbiol., 42, pp. 2806-2809; 
Jalaluddin, S., Devaster, J.-M., Scheen, R., Gerard, M., Butzler, J.-P., Molecular epidemiological study of nosocomial Enterobacter aerogenes isolates in a Belgian hospital (1998) J.Clin.Microbiol., 36, pp. 1846-1852; 
Juretschko, S., Labombardi, V.J., Lerner, S.A., Schreckenberger, P.C., Pseudomonas AST Study Group: Accuracies of β-lactam susceptibility test results for Pseudomonas aeruginosa with four automated systems (BD Phoenix, MicroScan WalkAway, Vitek, and Vitek 2) (2007) J.Clin.Microbiol., 45, pp. 1339-1342; 
King, E.O., Ward, M.K., Raney, D.E., Two simple media for the demonstration of pyocyanin and fluorescein (1954) J.Lab.Clin.Med., 44, pp. 301-307; 
Kiska, D.L., Gilligan, P.H., Pseudomonas and Burkholderia (1999) Manual of Clinical Microbiology, pp. 517-525. , in P.R.Murray,E.J.Baron,M.A.Pfaller,F.C.Tenover,R.H.Yolken(Eds) 7th ed. American Society for Microbiology,Washington (DC); 
Morales, G., Wiehlmann, L., Gudowius, P., van Delden, C., T{\"u}mmler, B., Mart{\'\i}nez, J.L., Rojo, F., Structure of Pseudomonas aeruginosa populations analyzed by single nucleotide polymorphism and pulsed-field gel electrophoresis genotyping (2004) J.Bacteriol., 186, pp. 4228-4237; 
Nogueira, J.A., (1995) Pseudomonas Aeruginosa - Agente Nosocomial, , (In Portuguese) PhD Thesis. Faculdade de Medicina, Universidade do Porto, Porto (Portugal); 
Olive, P.B.D., Principles and applications of methods for DNA-based typing of microbial organisms (1999) J.Clin.Microbiol., 37, pp. 1661-1669; 
Pirnay, J.P., de Vos, D., Cochez, C., Bilocq, F., Vanderkelen, A., Zizi, M., Ghysels, B., Cornelis, P., Pseudomonas aeruginosa displays an epidemic population structure (2002) Environ.Microbiol., 4, pp. 898-911; 
Pirnay, J.P., Matthijs, S., Colak, H., Chablain, P., Bilocq, F., Van eldere, J., de Vos, D., Cornelis, P., Global Pseudomonas aeruginosa biodiversity as reflected in a Belgian river (2005) Environ.Microbiol., 7, pp. 969-980; 
Speijer, H., Savelkoulp, P.H.M., Bonten, M.J., Stobberingh, E.E., Tjhie, J.H.T., Application of different genotyping methods for Pseudomonas aeruginosa in a setting of endemicity in an intensive care unit (1999) J.Clin.Microbiol., 37, pp. 3654-3661; 
Tenover, F., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H., Swaminathan, B., Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing (1995) J.Clin. Microbiol., 33, pp. 2233-2239; 
Wiehlmann, L., Wagner, G., Cramer, N., Siebert, B., Gudowius, P., Morales, G., K{\"o}hler, T., T{\"u}mmler, B., Population structure of Pseudomonas aeruginosa (2007) Proc.Nat.Acad.Sci.USA, 104, pp. 8101-8106
},
	month = {2008///},
	pages = {540 - 546},
	abstract = {The relatedness between clinical isolates of P. aeruginosa obtained from patients during their stay in a Portuguese Central Hospital was evaluated. Genotypic fingerprinting (M13-PCR), phenotypic methods (biotyping and antibiotyping) and epidemiological information (spatial and temporal links) were used to evaluate the relatedness between 88 clinical isolates (68 patients), selected randomly out of 189. Sixty-two M13 types were found, 12 of them containing isolates from more than one patient. Thirty-four antibiotypes were found, as well as a significant association (p < 0.05) between epidemic isolates and multiresistance patterns. The nosocomial transmission of P. aeruginosa strains may be limited since M13 typing demonstrated a high degree of diversity among all the isolates, suggesting the occurrence of mainly independent infectious episodes. The results show the possible occurrence of cross-acquisition, cross-colonization and cross-infection and suggest an epidemic population structure for P. aeruginosa in this hospital.},
	keywords = {antibiotic resistance, article, bacterial DNA, cross infection, disease transmission, DNA, Bacterial, Drug Resistance, Microbial, Electrophoresis, Gel, Pulsed-Field, epidemiology, genetics, genotype, Hospitals, Teaching, human, Humans, isolation and purification, microbiology, polymerase chain reaction, Portugal, Pseudomonas aeruginosa, Pseudomonas infection, Pseudomonas Infections, pulsed field gel electrophoresis, Sampling Studies, statistics, teaching hospital},
	isbn = {00155632 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-65549157108\&partnerID=40\&md5=ba6aed0b4b5f511f340fea96df7b6449},
	author = {Fonseca, A.P. and Correia, P. and Extremina, C.I. and Sousa, J.C. and Tenreiro, R. and Barros, H.}
}
