



@article {2527,
	title = {Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima: A new insight in the biomineralization field},
	journal = {European Journal of BiochemistryEur. J. Biochem.},
	volume = {269},
	year = {2002},
	note = {Cited By (since 1996): 58Export Date: 21 November 2012Source: ScopusCODEN: EJBCAdoi: 10.1046/j.1432-1033.2002.03203.xPubMed ID: 12383258Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiologie Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Amino Acids; Calcium, 7440-70-2; Edetic Acid, 60-00-4; Extracellular Matrix Proteins; Glycosaminoglycans; Insect Proteins; SilkReferences: Crenshaw, M.A., The soluble matrix from Mercenaria mercenaria shell (1972) Biomineralization, 6, pp. 6-11;Krampitz, G., Engels, J., Cazaux, C., Biochemical studies on water-soluble proteins and related components of gastropod shells (1976) The Mechanisms of Mineralization in the Invertebrates and Plants, pp. 175-190. , Watabe, N. \& Wilbur, K.M., eds. The Barush B.W. Library in Marine Science no. 5, The University of South Carolina Press, Columbia; 
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Atlan, G., Balmain, N., Berland, S., Vidal, B., Lopez, E., Reconstruction of human maxillary defects with nacre powder: Histological evidence for bone regeneration (1997) C. R. Acad. Sci. Paris, Life Sci., 320, pp. 253-258; 
Atlan, G., Delattre, O., Berland, S., Le Faou, A., Nabias, G., Cot, D., Lopez, E., Interface between bone and nacre implants in sheep (1999) Biomaterials, 20, pp. 1017-1022; 
Lamghari, M., Almeida, M.J., Berland, S., Huet, H., Laurent, A., Milet, C., Lopez, E., Stimulation of bone marrow cells and bone formation by nacre: In vivo and in vitro studies (1999) Bone, 25 S, pp. 91S-94S; 
Almeida, M.J., Milet, C., Peduzzi, J., Pereira, L., Haigle, J., Barth{\'e}lemy, M., Lopez, E., The effect of water-soluble matrix fraction extracted from the nacre of Pinctada maxima on the alkaline phosphatase activity of cultured fibroblasts (2000) J. Exp. Zool. (Mol. Dev. Evol.), 288, pp. 327-334; 
Almeida, M.J., Pereira, L., Milet, C., Haigle, J., Barbosa, M., Lopez, E., Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts (2001) J. Biomed. Mater. Res., 57, pp. 306-312; 
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},
	month = {2002///},
	pages = {4994 - 5003},
	abstract = {Nacre organic matrix has been conventionally classified as both {\textquoteright}water-soluble{\textquoteright} and {\textquoteright}water-insoluble{\textquoteright}, based on its solubility in aqueous solutions after decalcification with acid or EDTA. Some characteristics (aspartic acid-rich, silk-fibroin-like content) were specifically attributed to either one or the other. The comparative study on the technique of extraction (extraction with water alone vs. demineralization with EDTA) presented here, seems to reveal that this generally accepted classification may need to be reconsidered. Actually, the nondecalcified soluble organic matrix, extracted in ultra-pure water, displays many of the characteristics of what until now has been called {\textquoteright}insoluble matrix{\textquoteright}. We present the results obtained on this extract and on a conventional EDTA-soluble matrix, with various characterization methods: fractionation by size-exclusion and anion-exchange HPLC, amino acid analysis, glycosaminoglycan and calcium quantification, SDS/PAGE and FTIR spectroscopy. We propose that the model for the interlamellar matrix sheets of nacre given by Nakahara [In: Biomineralization and Biological Metal Accumulation, Westbroek, P. \& deJong, E.W., eds, (1983) pp. 225-230. Reidel, Dordrecht, Holland] and Weiner and Traub [Phil. Trans. R. Soc. Lond. B (1984) 304, 425-434] may no longer be valid. The most recent model, proposed by Levi-Kalisman et al. [J. Struct. Biol. (2001) 135, 8-17], seemed to be more in accordance with our findings.},
	keywords = {amino acid analysis, Amino acids, Animals, anion exchange chromatography, article, Biochemistry, biomineralization, bivalve, Bivalvia, Calcium, Chemical Fractionation, Chromatography, High Pressure Liquid, controlled study, demineralization, edetic acid, EDTA-soluble matrix, Electrophoresis, Polyacrylamide Gel, Extracellular Matrix Proteins, extraction, fractionation, gel permeation chromatography, glycosaminoglycan, Glycosaminoglycans, High performance liquid chromatography, hydrophobicity, infrared spectroscopy, Insect Proteins, model, Nacre, nonhuman, Ostreidae, Pinctada, Pinctada maxima, polyacrylamide gel electrophoresis, priority journal, Silk, Silk-fibroin-like-proteins, Solubility, Spectroscopy, Fourier Transform Infrared, Undecalcified soluble matrix, Water},
	isbn = {00142956 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036408881\&partnerID=40\&md5=7e99def46be711f02de5f4dd3dc8f63e},
	author = {Pereira-Mouri{\`e}s, L. and Almeida, M.-J. and Ribeiro, C. and Peduzzi, J. and Barth{\'e}lemy, M. and Milet, C. and Lopez, E.}
}
@article {2540,
	title = {Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {57},
	year = {2001},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/1097-4636(200111)57:2<306::AID-JBM1172>3.0.CO;2-HPubMed ID: 11484195Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiol. Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; BMP2 protein, human; Bmp2 protein, rat; bone morphogenetic protein 2; Bone Morphogenetic Proteins; Dexamethasone, 50-02-2; Glucocorticoids; Transforming Growth Factor betaReferences: Lopez, E., Vidal, B., Berland, S., Camprasse, S., Camprasse, G., Silve, C., Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro (1992) Tissue Cell, 24, pp. 667-679;Lopez, E., Berland, S., Le Faou, A., Nacre, osteogenic and osteoinductive properties (1994) Bull Inst Oc{\'e}an Monaco, 14, pp. 49-57; 
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},
	month = {2001///},
	pages = {306 - 312},
	abstract = {In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 μg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. {\textcopyright} 2001 John Wiley \& Sons, Inc.},
	keywords = {alkaline phosphatase, Animals, article, Biomedical engineering, BMP-2, Bone, bone development, bone marrow, Bone Marrow Cells, Bone morphogenetic proteins, Cell culture, Cell Line, cell proliferation, Cells, concentration response, dexamethasone, differentiation inducing factor, dose response, Dose-Response Relationship, Drug, enzyme activity, fibroblast, Fibroblasts, Glucocorticoids, human, human cell, Humans, male, MRC-5 fibroblasts, Nacre, natural product, osteoblast, Ostreidae, oyster, phenotype, Proteins, Pulmonary diseases, Rats, Rats, Wistar, Solubility, stroma cell, time, Time Factors, Transforming Growth Factor beta, unclassified drug, Water, Water-soluble matrix},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034769134\&partnerID=40\&md5=f0ec664a074b3a67745c806152793d54},
	author = {Almeida, M.J. and Pereira, L. and Milet, C. and Haigle, J. and Barbosa, M. and Lopez, E.}
}
