



@article {2540,
	title = {Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {57},
	year = {2001},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/1097-4636(200111)57:2<306::AID-JBM1172>3.0.CO;2-HPubMed ID: 11484195Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiol. Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; BMP2 protein, human; Bmp2 protein, rat; bone morphogenetic protein 2; Bone Morphogenetic Proteins; Dexamethasone, 50-02-2; Glucocorticoids; Transforming Growth Factor betaReferences: Lopez, E., Vidal, B., Berland, S., Camprasse, S., Camprasse, G., Silve, C., Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro (1992) Tissue Cell, 24, pp. 667-679;Lopez, E., Berland, S., Le Faou, A., Nacre, osteogenic and osteoinductive properties (1994) Bull Inst Oc{\'e}an Monaco, 14, pp. 49-57; 
Silve, C., Lopez, E., Vidal, B., Smith, D.C., Camprasse, S., Camprasse, G., Couly, G., Nacre initiates biomineralization by human osteoblasts maintained in vitro (1992) Calcif Tissue Int, 51, pp. 363-369; 
Atlan, G., Balmain, N., Berland, S., Vidal, B., Lopez, E., Reconstruction of human maxillary defects with nacre powder: Histological evidence for bone regeneration (1997) C R Acad Sci III, 320, pp. 253-258; 
Atlan, G., Delattre, O., Berland, S., Le Faou, A., Nabias, G., Cot, D., Lopez, E., Interface between bone and nacre implants in sheep (1999) Biomaterials, 20, pp. 1017-1022; 
Lamghari, M., Almeida, M.J., Berland, S., Huet, H., Laurent, A., Milet, C., Lopez, E., Stimulation of bone marrow cells and bone formation by nacre: In vivo and in vitro studies (1999) Bone, 25 S, pp. 91S-94S; 
Westbroek, P., Marin, F., A marriage of bone and nacre (1998) Nature, 392, pp. 861-862; 
Le Faou, A., Berland, S., Lallier, F., Lopez, E., Osteoinductive effects induced by nacre implanted in subcutaneous pouches in rat (1995) Calcif Tissue Int, 56 A, p. 156; 
Le Faou, A., Berland, S., Lallier, F., Lopez, E., Local regulation of the osteoinductive process induced by nacre in connective tissues (1996) Ann Endoc, 57 (SUPPL. 4A), p. 75; 
Aubin, J.E., Liu, F., Malaval, L., Gupta, A.K., Osteoblast and chondroblast differentiation (1995) Bone, (SUPPL. 2), pp. S77-S83; 
Huggins, C.B., Urist, M.R., Dentin matrix transformation: Rapid induction of alkaline phosphatase and cartilage (1970) Science, 167, pp. 896-897; 
Reddi, A.H., Huggins, C.B., Biochemical sequences in the transformation of normal fibroblasts in adolescent rats (1972) Proc Natl Acad Sci USA, 69, pp. 1601-1605; 
Glowacki, J., Mulliken, J.B., Demineralized bone implants (1985) Clin Plast Surg, 12 (2), pp. 233-241; 
Katagiri, T., Yamaguchi, A., Ikeda, T., Yoshiki, S., Wozney, J.M., Rosen, V., Wang, E.A., Suda, T., The nonosteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteobtastic cells by recombinant human bone morphogenetic protein-2 (1990) Biochem Biophys Res Commun, 172, pp. 295-299; 
McCulloch, C.A.G., Tenenbaum, H.C., Dexamethasone induces proliferation and terminal differentiation of osteogenic cells in tissue culture (1986) Anat Rec, 215, pp. 397-402; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Bellows, C.G., Aubin, J.E., Determination of numbers of osteoprogenitors present in isolated fetal calvarial cells in vitro (1989) Dev Biol, 133, pp. 8-13; 
Bhargava, U., Bar-Lev, M., Bellows, C.G., Aubin, J.E., Ultrastructural analyses of bone nodules formed in vitro by isolated fetal rat calvaria cells (1988) Bone, 9, pp. 155-163; 
Wiren, K.M., Rodan, G.A., Dissociation between the effects of glucocorticosteroids on cell spreading and phenotypic expression (1985) The chemistry and biology of mineralized tissues, pp. 320-325. , Butler WT, editor. Birmingham, AL: EBSCO Media; 
Kasugai, S., Todescan, R., Nagata, T., Yao, K.-L., Butler, W.T., Sodek, J., Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro: Inductive effects of dexamethasone on the osteoblastic phenotype (1991) J Cell Physiol, 147, pp. 111-120; 
Chen, T.L., Bates, R.L., Dudley, A., Hammonds, R.G., Amento, E.P., Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteobtast-like cells: Comparison with TGF-β1 (1991) J Bone Miner Res, 6, pp. 1387-1393; 
Hiraki, Y., Inoue, H., Shigeno, C., Sanma, Y., Bentz, H., Rosen, D.M., Asada, A., Suzuki, F., Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitro (1991) J Bone Miner Res, 6, pp. 1373-1385; 
Rosen, V., Thies, R.S., The BMP proteins in bone formation and repair (1992) Trends Genet, 8, pp. 97-102; 
Thies, R.S., Bauduy, M., Ashton, B.A., Kurtzberg, L., Wozney, J.M., Rosen, V., Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells (1992) Endocrinology, 130, pp. 1318-1324; 
Urist, M.R., Iwata, H., Ceccotti, P.L., Dorfman, R.L., Boyd, S.D., McDowell, R., Chien, C., Bone morphogenesis in implants of insoluble bone gelatin (1973) Proc Natl Acad Sci USA, 70, pp. 3511-3515; 
Almeida, M.J., Milet, C., Peduzzi, J., Pereira, L., Haigle, J., Barth{\'e}lemy, M., Lopez, E., The effect of water-soluble matrix fraction extracted from the nacre of Pinctada maxima on the alkaline phosphatase activity of cultured fibroblasts (2000) J Exp Zool, 288, pp. 327-334; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Owen, M., Marrow stromal stem cells (1988) J Cell Sci, (SUPPL. 10), pp. 63-76; 
Triffitt, J.T., Initiation and enhancement of bone formation (1987) Acta Orthop Scand, 58, pp. 673-684; 
Triffitt, J.T., The stem cell of the osteoblast (1996) Principles of bone biology, pp. 39-50. , Bilezikian JP, Raisz LG, Rodan GA, editors. New York: Academic Press; 
Wharton, S.A., Hipkiss, A.R., Degradation of peptides and proteins of different sizes by homogenates of human MRC5 lung fibroblasts (1985) FEBS Lett, 184 (2), pp. 249-253; 
Harada, K., Oida, S., Sasaki, S., Chondrogenesis and osteogenesis of bone marrow-derived cells by bone-inductive factor (1988) Bone, 9, pp. 177-183; 
Tucker, R.F., Shipley, G.D., Moses, H.L., Growth inhibitor from BSC-1 cells closely related to platelet type β transforming factor (1984) Science, 226, pp. 705-707; 
Noda, M., Rodan, G.A., Type-β transforming growth factor inhibits proliferation and expression of alkaline phosphatase in murine osteoblast-like cells (1986) Biochem Biophys Res Commun, 140, pp. 56-65; 
Tintut, Y., Parhami, F., Bostr{\"o}m, K., Jackson, S., Demer, L., cAMP stimulates osteoblast-like differentiation of calcifying vascular cells (1998) J Biol Chem, 273, pp. 7547-7553; 
Rickard, D.J., Sullivan, T.A., Shenker, B.J., Leboy, P.S., Kazhdan, I., Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2 (1994) Dev Biol, 161, pp. 218-228; 
Coelho, M.J., Fernandes, M.H., Human bone cell cultures in biocompatibility testing. Part II. Effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation (2000) Biomaterials, 21, pp. 1095-1102; 
Green, S., Chambon, P., A superfamily of potentially oncogenic hormone receptors (1986) Nature, 324, pp. 615-617; 
Grigoriadis, A.E., Heersche, J.N.M., Aubin, J.E., Differentiation of muscle, fat, cartilage and bone from progenitor cells present in a bone-derived clonal cell population: Effect of dexamethasone (1988) J Cell Biol, 106, pp. 2139-2151; 
Leboy, P.S., Beresford, J.N., Devlin, C., Owen, M.E., Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures (1991) J Cell Physiol, 146, pp. 370-378; 
Guerriero V., Jr., Florini, J.R., Dexamethasone effects on myoblast proliferation and differentiation (1980) Endocrinology, 106, pp. 1198-1202; 
Maor, G., Silbermann, M., Supraphysiological concentrations of dexamethasone induce elevation of calcium uptake and depression of [ 3H]-thymidine incorporation into DNA in cartilage in vitro (1986) Calcif Tissue Int, 39, pp. 284-290; 
Urist, M.R., Bone morphogenetic protein in biology and medicine (1996) Bone morphogenetic proteins: Biology, biochemistry and reconstructive surgery, pp. 7-27. , Lindholm TS, editor. Austin, TX: RG Landes Company; 
Wozney, J.M., Rosen, V., Byrne, T., Celeste, A.J., Moutsatsos, I., Wang, E.A., Growth factor influencing bone development (1990) J Cell Sci, 97 (SUPPL. 13), pp. 149-156; 
Takuwa, Y., Ohse, C., Wang, E.A., Wozney, J.M., Yamashita, K., Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3 (1991) Biochem Biophys Res Commun, 174, pp. 96-101; 
Jortikka, L., Target cells and receptors for bone morphogenetic protein (1996) Bone morphogenetic proteins: Biology, biochemistry and reconstructive surgery, pp. 65-73. , Lindholm TS, editor. Austin, TX: RG Landes Company; 
Canalis, E., Centrella, M., Urist, M.R., Effect of partially purified bone morphogenetic protein on DNA synthesis and cell replication in calvarial and fibroblast cultures (1985) Clin Orthop, 198, pp. 289-294; 
Centrella, M., Casinghino, S., Kim, J., Pham, T., Rosen, V., Wozney, J., McCarthy, T.L., Independent changes in type I and type II receptors for transforming growth factor β induced by bone morphogenetic protein 2 parallel expression of the osteoblast phenotype (1995) Mol Cell Biol, 15, pp. 3273-3281; 
Vukicevic, S., Luyten, F.P., Redi, A.H., Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin (1989) Proc Natl Acad Sci USA, 86, pp. 8793-8797
},
	month = {2001///},
	pages = {306 - 312},
	abstract = {In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 μg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. {\textcopyright} 2001 John Wiley \& Sons, Inc.},
	keywords = {alkaline phosphatase, Animals, article, Biomedical engineering, BMP-2, Bone, bone development, bone marrow, Bone Marrow Cells, Bone morphogenetic proteins, Cell culture, Cell Line, cell proliferation, Cells, concentration response, dexamethasone, differentiation inducing factor, dose response, Dose-Response Relationship, Drug, enzyme activity, fibroblast, Fibroblasts, Glucocorticoids, human, human cell, Humans, male, MRC-5 fibroblasts, Nacre, natural product, osteoblast, Ostreidae, oyster, phenotype, Proteins, Pulmonary diseases, Rats, Rats, Wistar, Solubility, stroma cell, time, Time Factors, Transforming Growth Factor beta, unclassified drug, Water, Water-soluble matrix},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034769134\&partnerID=40\&md5=f0ec664a074b3a67745c806152793d54},
	author = {Almeida, M.J. and Pereira, L. and Milet, C. and Haigle, J. and Barbosa, M. and Lopez, E.}
}
@article {2615,
	title = {Amino acid and metal content of Crassostrea gigas shell infested by Polydora sp. in the prismatic layer insoluble matrix and blister membrane},
	journal = {Aquatic Living ResourcesAquatic Living Resour.},
	volume = {9},
	year = {1996},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: ALREELanguage of Original Document: EnglishCorrespondence Address: Almeida, M.J.; Laborat{\'o}rio de Fisiologia Aplicada, Instituto de Ci{\^e}ncias Biom{\'e}dicas Abel Salazar, 4050 Porto, PortugalReferences: Allen, J.A., Manganese deposition on the shell of living molluscs (1960) Nature, 185, pp. 336-337;Beedham, G.E., Repair of the shell in species of Anodonta (1965) Proc. Zool. Soc. London, 145, pp. 107-125; 
Boggess, R.K., Martin, R.B., Copper chelation by DOPA, epinephrine and other catechols (1975) J. Am. Soc. Chem., 97, pp. 3076-3081; 
Brown, C.H., (1975) Structural Materials in Animals, , Wiley, New York; 
Bubel, A., An electron microscope study of periostracum repair in Mytilus edulis (1973) Mar. Biol., 20, pp. 235-244; 
Carriker, M.R., Swann, C.P., Prezant, R.S., Counts, C.L., Chemical elements in the aragonitic and calcitic microstructural groups of shell of the oyster Crassostrea virginica: A proton probe study (1991) Mar. Biol., 109, pp. 289-297; 
Catherine, M., Blateau, D., Mazuri{\'e}, J., Le Bec, C., (1990) Anomalies des Coquilles D{\textquoteright}hu{\^\i}tres Creuses Crassostrea Gigas Observ{\'e}es sur le Littoral Fran{\c c}ais en Mai-juin 1989, Dues au ver Polydora et aux Peintures Antisalissures., pp. 13-14. , Rapp. Int. Dir. Ressources Vivantes, IFREMER Nantes; 
Degens, E.T., Spencer, D.W., Parker, R.H., Paleobiochemistry of Molluscan shell proteins (1967) Comp. Biochem. Physiol., 79, pp. 553-579; 
Dorsett, D.A., The behavior of Polydora ciliata (Johnst.). Tube building and burrowing (1961) J. Mar. Biol. Assoc. U.K., 41, pp. 577-590; 
Felix, C.C., Sealy, R.C., Electron spin resonance characterization of radicals from 3-4-dihydroxyphenylalanine: Semiquinone anions and their metal chelates (1981) J. Am. Chem. Soc., 103, pp. 2831-2835; 
Galstoff, P.S., The American oyster Crassostrea virginica (Gmelin) (1964) Fish. Bull. U.S., p. 64; 
Gergely, A., Kiss, T., Coordination chemistry of L-DOPA and related ligands (1979) Met. Ions Biol. Syst., 9, pp. 143-172; 
Haigler, S., Boring mechanism of Polydora websteri inhabiting Crassostrea virginica (1969) Am. Zool., 9, pp. 821-828; 
Jones, G.M., Saleuddin, A.S.M., Cellular mechanisms of periostracum formation in Physa spp. (Mollusca: Pulmonata) (1978) Can. J. Zool., 56, pp. 2299-2311; 
Kawaguchi, T., Watabe, N., The organic matrices of the shell of the American oyster Crassostrea virginica Gmelin (1993) J. Exp. Mar. Biol. Ecol., 170, pp. 11-28; 
Kawaguti, S., Ikemoto, N., Electron microscopy on the mantle of a bivalve Musculus senhousia, during regeneration of shell (1962) Biol. J. Okayama Univ., 8, pp. 31-42; 
Krampitz, G., Drolshagen, H., Hotta, S., Simultaneous binding of calcium and bicarbonate by conchiolin of oyster shells (1983) Experiemia, 39, pp. 1104-1105; 
Laukner, G., Diseases of Mollusca: Bivalvia (1983) Diseases of Marine Animals, Vol. II, Bivalvia to Scaphopoda, 2, pp. 477-978. , Biologische Anstalt Helgoland, O. Kinne ed. Hamburg; 
Levitt, M., A simplified representation of protein conformation for rapid simulation of protein folding (1976) J. Mol. Biol., 104, pp. 59-108; 
Linderstrom-Lang, K., Duspiva, F., Studies in enzymatic histochemistry. XVI. the digestion of keratin by larvae of clothes moth (Tineola biselliela Humm.) (1936) C. R. Trav. Lab. Carlsberg, Ser. Chim., 21, pp. 1-82; 
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein determination with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; 
Martius, K.V., Schwarzhans, K.E., Preparation of the Mn (II) complex with 3,4-dihydroxyphenylalanine (DOPA) (1978) Z Naturforsch., 33 B, p. 124; 
Meenaakshi, V.R., Hare, P.E., Watabe, N., Wilbur, K.M., The chemical composition of the periostracum of the molluscan shell (1969) Comp. Biochem. Physiol., 29, pp. 611-620; 
Nozaki, Y., Tanford, C., The solubility of amino acids and two glycyl peptides in aqueous ethanol and dioxane solutions (1971) J. Biol. Chem., 246, pp. 2211-2217; 
Saleuddin, A.S.M., Petit, H.P., The mode of formation and the structure of the periostracum (1983) The Mollusca, 4, pp. 199-234. , K. M. Wilbur ed. Academic Press, New York; 
Sato-Okoshi, W., Okoshi, K., Microstructure of scallop and oyster shells infested with boring Polydora (1993) Nippon Suisan Gakkaishi, 59, pp. 1243-1247; 
Segar, D.A., Collins, J.D., Riley, J.P., The distribution of the major and some minor elements in marine animals. Part II (1971) Molluscs. J. Mar. Biol. Assoc. U.K., 51, pp. 131-136; 
Swinehart, J.H., Smith, K.W., Iron and manganese deposition in the periostraca of several bivalve molluscs (1979) Biol. Bull., 156, pp. 369-381; 
Travis, D.F., Gonsalves, M., Comparative ultrastructure and organization of the prismatic region of two bivalves and its possible relation to the chemical mechanism of boring (1969) Am. Zool., 9, pp. 635-661; 
Tsujii, T., An electron microscopic study of the mantle epithelial cells of Anodonta sp. during shell regeneration (1976) The Mechanisms of Mineralization in the Invertebrates and Plants, pp. 339-353. , N. Watabe, K. M. Wilbur eds. Univ. South Carolina Press, Columbia; 
Vovelle, J., Scl{\'e}rotisation et min{\'e}ralisation des structures squellettiques chez les Mollusques (1974) Haliotis, 2, pp. 133-165; 
Waite, J.H., Evidence for the mode of sclerotization in a molluscan periostracum (1977) Comp. Biochem. Physiol., 58 B, pp. 157-162; 
Waite, J.H., Quinone-tanned scleroproteins (1983) The Mollusca, 1, pp. 467-504. , K. M. Wilbur ed. Academic Press, New York; 
Waite, J.H., Andersen, S.O., 3,4-dihydroxyphenyl-alanine in an insoluble shell protein of Mytilus edulis (1978) Biochem. Biophys. Acta, 541, pp. 107-114; 
Wheeler, A.P., Rusenko, K.W., Swift, D.M., Sikes, C.S., Regulation of in vitro and in vivo CaCO 3 crystallization by fractions of oyster shell organic matrix (1988) Mar. Biol., 98, pp. 71-80; 
Zottoli, R.A., Carriker, M.R., Burrow morphology, tube formation and microarchitecture of shell dissolution by the spionid polychaete Polydora websteri (1974) Mar. Biol., 27, pp. 307-316
},
	month = {1996///},
	pages = {179 - 186},
	abstract = {The spionid polychaete Polydora sp. can live and reproduce inside the oysters{\textquoteright} shell, excavating a U-shaped burrow the central portion of which is filled with detritus and particles of dissolved shell. The oyster responds by secreting an organic layer, confining the worm, and later covering it with calcitostracum. This process creates a characteristic "mud blister". Crassostrea gigas were analysed for the organic matrix amino acid content and 14 metals of the whole shell of normal oysters and severely Polydora infested ones. Amino acid, DOPA and FT-Raman analyses were also performed on isolated membranes from the characteristic mud blister and on prismatic layer insoluble matrix (PLIM) of infested oysters, with the aim of detecting any differences between the two organic matrices. Membranes and PLIM composition differ from whole normal shell organic matrix by having lower aspartate and glycine and higher levels of virtually all hydrophobic amino acids, especially alanine. There are no significant differences between membranes and PLIM in the ratio of charged to non-polar amino acids, respectively 0.54 and 0.59. L-DOPA content is also similar in the two matrices with 0.45 ({\textpm}0.18) and 0.57 ({\textpm}0.30) ng.mg},
	keywords = {Amino acids, Crassostrea gigas, Metals, Polydora sp., Shell organic matrix},
	isbn = {09907440 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0008495129\&partnerID=40\&md5=80ed4fe02d098797dec36a0481fbbecf},
	author = {Almeida, M.J. and Moura, G. and MacHado, J. and Coimbra, J. and Vilarinho, L. and Ribeiro, C. and Soares-Da-Silva, P.}
}
