



@article {3273,
	title = {Characterization of hLF1-11 immobilization onto chitosan ultrathin films, and its effects on antimicrobial activity},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {3513 - 3521},
	abstract = {hLF1-11 (GRRRRSVQWCA) is an antimicrobial peptide (AMP) with high activity against methicillin-resistant Staphylococcus aureus (MRSA), the most prevalent species in implant-associated infection. In this work, the effect of the surface immobilization on hLF1-11 antimicrobial activity was studied. Immobilization was performed onto chitosan thin films as a model for an implant coating due to its reported osteogenic and antibacterial properties. Chitosan thin films were produced by spin-coating on gold surfaces. hLF1-11 was immobilized onto these films by its C-terminal cysteine in an orientation that exposes the antimicrobial activity-related arginine-rich portion of the peptide. Two levels of exposure (with and without a polyethylene glycol (PEG) spacer) were analyzed. Covalent immobilization was further compared with the AMP physical adsorption onto chitosan films. Surfaces were characterized using ellipsometry, contact angle measurements, atomic force microscopy, infrared and X-ray photoelectron spectroscopies and using a fluorimetric assay for hLF1-11 quantification. Surface antimicrobial activity was assessed through surface adhesion and viability assays using an MRSA (S. aureus ATCC 33591). The incorporation of hLF1-11 increased significantly bacterial adhesion to chitosan films. However, the presence of hLF1-11, namely when immobilized through a PEG spacer, decreased the viability of adherent bacteria with regard to the control surface. These results demonstrated that hLF1-11 after covalent immobilization by its cysteine can maintain activity, particularly if a spacer is applied. However, further studies, exploring the opposite orientation or the same C-terminal orientation, but non-cysteine related, can help to clarify the potential of the hLF1-11 immobilization strategy. {\textcopyright} 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Antimicrobial peptides (AMP), Bacterial adhesion, chitosan, Surface characterization, Surface immobilization},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903724424\&partnerID=40\&md5=e8009cd480b49c37a991753cd145cf34},
	author = {Costa, F. and Maia, S. and Gomes, J. and Gomes, P. and Martins, M.C.L.}
}
@article {2236,
	title = {Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2010.11.005PubMed ID: 21056701Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; carbenicillin, 17230-86-3, 4697-36-3, 4800-94-6; cefazolin, 25953-19-9, 27164-46-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; penicillin G, 1406-05-9, 61-33-6; teicoplanin, 61036-62-2, 61036-64-4; tobramycin, 32986-56-4; vancomycin, 1404-90-6, 1404-93-9; Antimicrobial Cationic Peptides; Biocompatible Materials; Coated Materials, Biocompatible; Immobilized ProteinsReferences: Glinel, K., Jonas, A.M., Jouenne, T., Leprince, J., Galas, L., Huck, W.T., Antibacterial and antifouling polymer brushes incorporating antimicrobial peptide (2009) Bioconj Chem, 20 (1), pp. 71-77;Vila, J., Soriano, A., Mensa, J., Molecular basis of microbial adherence to prosthetic materials. Role of biofilms in prosthesis-associated infection (2008) Enferm Infecc Microbiol Clin, 26 (1), pp. 48-54; 
Hetrick, E.M., Schoenfisch, M.H., Reducing implant-related infections: Active release strategies (2006) Chemical Society Reviews, 35 (9), pp. 780-789. , DOI 10.1039/b515219b; 
Darouiche, R.O., Treatment of Infections Associated with Surgical Implants (2004) New England Journal of Medicine, 350 (14), pp. 1422-1429. , DOI 10.1056/NEJMra035415; 
Zhao, L., Chu, P.K., Zhang, Y., Wu, Z., Antibacterial coatings on titanium implants (2009) J Biomed Mater Res B Appl Biomater, 91 (1), pp. 470-480; 
Dee, K.C., Puleo, D.A., Bizios, R., (2002) An Introduction to Tissue-biomaterial Interactions, , Wiley-Liss New York; 
Subramani, K., Jung, R.E., Molenberg, A., Hammerle, C.H., Biofilm on dental implants: A review of the literature (2009) Int J Oral Maxillofac Implants, 24 (4), pp. 616-626; 
Fey, P.D., Olson, M.E., Current concepts in biofilm formation of Staphylococcus epidermidis (2010) Future Microbiol, 5 (6), pp. 917-933; 
De Carvalho, C.C., Biofilms: Recent developments on an old battle (2007) Recent Pat Biotechnol, 1 (1), pp. 49-57; 
Costerton, J.W., Stewart, P.S., Greenberg, E.P., Bacterial biofilms: A common cause of persistent infections (1999) Science, 284 (5418), pp. 1318-1322. , DOI 10.1126/science.284.5418.1318; 
Willcox, M.D., Hume, E.B., Aliwarga, Y., Kumar, N., Cole, N., A novel cationic-peptide coating for the prevention of microbial colonization on contact lenses (2008) J Appl Microbiol, 105 (6), pp. 1817-1825; 
Lewis, K., Riddle of biofilm resistance (2001) Antimicrobial Agents and Chemotherapy, 45 (4), pp. 999-1007. , DOI 10.1128/AAC.45.4.999-1007.2001; 
Monteiro, D.R., Gorup, L.F., Takamiya, A.S., Ruvollo-Filho, A.C., De Camargo, E.R., Barbosa, D.B., The growing importance of materials that prevent microbial adhesion: Antimicrobial effect of medical devices containing silver (2009) Int J Antimicrob Agents, 34 (2), pp. 103-110; 
Gooderham, W.J., Bains, M., McPhee, J.B., Wiegand, I., Hancock, R.E., Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation, and swarming motility of PsrA in Pseudomonas aeruginosa (2008) J Bacteriol, 190 (16), pp. 5624-5634; 
Bagge, N., Schuster, M., Hentzer, M., Ciofu, O., Givskov, M., Greenberg, E.P., Hoiby, N., Pseudomonas aeruginosa Biofilms Exposed to Imipenem Exhibit Changes in Global Gene Expression and β-Lactamase and Alginate Production (2004) Antimicrobial Agents and Chemotherapy, 48 (4), pp. 1175-1187. , DOI 10.1128/AAC.48.4.1175-1187.2004; 
Ahmed, N.A., Petersen, F.C., Scheie, A.A., AI-2/LuxS is involved in increased biofilm formation by Streptococcus intermedius in the presence of antibiotics (2009) Antimicrob Agents Chemother, 53 (10), pp. 4258-4263; 
Hoffman, L.R., D{\textquoteright}Argenio, D.A., MacCoss, M.J., Zhang, Z., Jones, R.A., Miller, S.I., Aminoglycoside antibiotics induce bacterial biofilm formation (2005) Nature, 436 (7054), pp. 1171-1175. , DOI 10.1038/nature03912; 
Harbers, G.M., Emoto, K., Greef, C., Metzger, S.W., Woodward, H.N., Mascali, J.J., Grainger, D.W., Lochhead, M.J., Functionalized poly(ethylene glycol)-based bioassay surface chemistry that facilitates bio-immobilization and inhibits nonspecific protein, bacterial, and mammalian cell adhesion (2007) Chemistry of Materials, 19 (18), pp. 4405-4414. , DOI 10.1021/cm070509u; 
Chapman, R.G., Ostuni, E., Liang, M.N., Meluleni, G., Kim, E., Yan, L., Pier, G., Whitesides, G.M., Polymeric thin films that resist the adsorption of proteins and the adhesion of bacteria (2001) Langmuir, 17 (4), pp. 1225-1233. , DOI 10.1021/la001222d; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res A, 89 (3), pp. 642-653; 
Chung, D., Papadakis, S.E., Yam, K.L., Evaluation of a polymer coating containing triclosan as the antimicrobial layer for packaging materials (2003) International Journal of Food Science and Technology, 38 (2), pp. 165-169. , DOI 10.1046/j.1365-2621.2003.00657.x; 
Leung, D., Spratt, D.A., Pratten, J., Gulabivala, K., Mordan, N.J., Young, A.M., Chlorhexidine-releasing methacrylate dental composite materials (2005) Biomaterials, 26 (34), pp. 7145-7153. , DOI 10.1016/j.biomaterials.2005.05.014, PII S0142961205003881; 
Ravikumar, T., Murata, H., Koepsel, R.R., Russell, A.J., Surface-active antifungal polyquaternary amine (2006) Biomacromolecules, 7 (10), pp. 2762-2769. , DOI 10.1021/bm060476w; 
Bach, A., Darby, D., Bottiger, B., Bohrer, H., Motsch, J., Martin, E., Retention of the antibiotic teicoplanin on a hydromer-coated central venous catheter to prevent bacterial colonization in postoperative surgical patients (1996) Intensive Care Medicine, 22 (10), pp. 1066-1069. , DOI 10.1007/s001340050214; 
Jose, B., Antoci Jr., V., Zeiger, A.R., Wickstrom, E., Hickok, N.J., Vancomycin covalently bonded to titanium beads kills Staphylococcus aureus (2005) Chemistry and Biology, 12 (9), pp. 1041-1048. , DOI 10.1016/j.chembiol.2005.06.013, PII S1074552105002309; 
Kilic, D., Agalar, C., Ozturk, E., Denkbas, E.B., Cime, A., Agalar, F., Antimicrobial activity of cefazolin-impregnated mesh grafts (2007) ANZ Journal of Surgery, 77 (4), pp. 256-260. , DOI 10.1111/j.1445-2197.2007.04029.x; 
Gallardo-Moreno, A.M., Pacha-Olivenza, M.A., Saldana, L., Perez-Giraldo, C., Bruque, J.M., Vilaboa, N., In vitro biocompatibility and bacterial adhesion of physico-chemically modified Ti6Al4V surface by means of UV irradiation (2009) Acta Biomater, 5 (1), pp. 181-192; 
Jansen, B., Kohnen, W., Prevention of biofilm formation by polymer modification (1995) Journal of Industrial Microbiology, 15 (4), pp. 391-396. , DOI 10.1007/BF01569996; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly (hydroxyethylmethacrylate)) surfaces (2003) Journal of Biomaterials Science, Polymer Edition, 14 (5), pp. 439-455. , DOI 10.1163/156856203766652057; 
Zhang, F., Zhang, Z., Zhu, X., Kang, E.T., Neoh, K.G., Silk-functionalized titanium surfaces for enhancing osteoblast functions and reducing bacterial adhesion (2008) Biomaterials, 29 (36), pp. 4751-4759; 
Flemming, R.G., Capelli, C.C., Cooper, S.L., Proctor, R.A., Bacterial colonization of functionalized polyurethanes (2000) Biomaterials, 21 (3), pp. 273-281; 
Singla, A.K., Chawla, M., Chitosan: Some pharmaceutical and biological aspects - An update (2001) Journal of Pharmacy and Pharmacology, 53 (8), pp. 1047-1067. , DOI 10.1211/0022357011776441; 
Murata, H., Koepsel, R.R., Matyjaszewski, K., Russell, A.J., Permanent, non-leaching antibacterial surfaces-2: How high density cationic surfaces kill bacterial cells (2007) Biomaterials, 28 (32), pp. 4870-4879. , DOI 10.1016/j.biomaterials.2007.06.012, PII S0142961207004681; 
Aumsuwan, N., Danyus, R.C., Heinhorst, S., Urban, M.W., Attachment of ampicillin to expanded poly(tetrafluoroethylene): Surface reactions leading to inhibition of microbial growth (2008) Biomacromolecules, 9 (7), pp. 1712-1718; 
Cevher, E., Orhan, Z., Mulazimoglu, L., Sensoy, D., Alper, M., Yildiz, A., Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus with prepared microspheres (2006) Int J Pharm, 317 (2), pp. 127-135; 
Faber, C., Stallmann, H.P., Lyaruu, D.M., Joosten, U., Von Eiff, C., Van Nieuw Amerongen, A., Wuisman, P.I.J.M., Comparable efficacies of the antimicrobial peptide human lactoferrin 1-11 and gentamicin in a chronic methicillin-resistant Staphylococcus aureus osteomyelitis model (2005) Antimicrobial Agents and Chemotherapy, 49 (6), pp. 2438-2444. , DOI 10.1128/AAC.49.6.2438-2444.2005; 
Aumsuwan, N., Heinhorst, S., Urban, M.W., Antibacterial surfaces on expanded polytetrafluoroethylene; penicillin attachment (2007) Biomacromolecules, 8 (2), pp. 713-718. , DOI 10.1021/bm061050k; 
Palumbi, S.R., Humans as the world{\textquoteright}s greatest evolutionary force (2001) Science, 293 (5536), pp. 1786-1790. , DOI 10.1126/science.293.5536.1786; 
Andreu, D., Rivas, L., Animal antimicrobial peptides: An overview (1998) Biopolymers, 47 (6), pp. 415-433; 
Rivas, L., Luque-Ortega, J.R., Andreu, D., Amphibian antimicrobial peptides and Protozoa: Lessons from parasites (2009) Biochim Biophys Acta, 1788 (8), pp. 1570-1581; 
Gordon, Y.J., Romanowski, E.G., McDermott, A.M., Mini review: A review of antimicrobial peptides and their therapeutic potential as anti-infective drugs (2005) Current Eye Research, 30 (7), pp. 505-515. , DOI 10.1080/02713680590968637; 
Guani-Guerra, E., Santos-Mendoza, T., Lugo-Reyes, S.O., Teran, L.M., Antimicrobial peptides: General overview and clinical implications in human health and disease (2010) Clin Immunol, 135 (1), pp. 1-11; 
Perron, G.G., Zasloff, M., Bell, G., Experimental evolution of resistance to an antimicrobial peptide (2006) Proceedings of the Royal Society B: Biological Sciences, 273 (1583), pp. 251-256. , DOI 10.1098/rspb.2005.3301; 
Reddy, K.V.R., Yedery, R.D., Aranha, C., Antimicrobial peptides: Premises and promises (2004) International Journal of Antimicrobial Agents, 24 (6), pp. 536-547. , DOI 10.1016/j.ijantimicag.2004.09.005, PII S092485790400322X; 
Van {\textquoteright}T Hof, W., Veerman, E.C.I., Heimerhorst, E.J., Nieuw Amerongen, A.V., Antimicrobial peptides: Properties and applicability (2001) Biological Chemistry, 382 (4), pp. 597-619. , DOI 10.1515/BC.2001.072; 
Li, M., Cha, D.J., Lai, Y., Villaruz, A.E., Sturdevant, D.E., Otto, M., The antimicrobial peptide-sensing system aps of Staphylococcus aureus (2007) Molecular Microbiology, 66 (5), pp. 1136-1147. , DOI 10.1111/j.1365-2958.2007.05986.x; 
Li, M., Lai, Y., Villaruz, A.E., Cha, D.J., Sturdevant, D.E., Otto, M., Gram-positive three-component antimicrobial peptide-sensing system (2007) Proceedings of the National Academy of Sciences of the United States of America, 104 (22), pp. 9469-9474. , DOI 10.1073/pnas.0702159104; 
Otto, M., Bacterial sensing of antimicrobial peptides (2009) Contrib Microbiol, 16, pp. 136-149; 
Peschel, A., Vincent Collins, L., Staphylococcal resistance to antimicrobial peptides of mammalian and bacterial origin (2001) Peptides, 22 (10), pp. 1651-1659. , DOI 10.1016/S0196-9781(01)00500-9, PII S0196978101005009; 
Peschel, A., Jack, R.W., Otto, M., Collins, L.V., Staubitz, P., Nicholson, G., Kalbacher, H., Van Strijp, J.A.G., Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with L-lysine (2001) Journal of Experimental Medicine, 193 (9), pp. 1067-1076. , DOI 10.1084/jem.193.9.1067; 
Cao, M., Helmann, J.D., The Bacillus subtilis Extracytoplasmic-Function σ X Factor Regulates Modification of the Cell Envelope and Resistance to Cationic Antimicrobial Peptides (2004) Journal of Bacteriology, 186 (4), pp. 1136-1146. , DOI 10.1128/JB.186.4.1136-1146.2004; 
Clarke, S.R., Foster, S.J., IsdA protects Staphylococcus aureus against the bactericidal protease activity of apolactoferrin (2008) Infection and Immunity, 76 (4), pp. 1518-1526. , DOI 10.1128/IAI.01530-07; 
Vuong, C., Voyich, J.M., Fischer, E.R., Braughton, K.R., Whitney, A.R., DeLeo, F.R., Otto, M., Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system (2004) Cellular Microbiology, 6 (3), pp. 269-275. , DOI 10.1046/j.1462-5822.2004.00367.x; 
Zasloff, M., Antimicrobial peptides of multicellular organisms (2002) Nature, 415 (6870), pp. 389-395. , DOI 10.1038/415389a; 
Hancock, R.E.W., Peptide antibiotics (1997) Lancet, 349 (9049), pp. 418-422. , DOI 10.1016/S0140-6736(97)80051-7; 
Cho, W.-M., Joshi, B.P., Cho, H., Lee, K.-H., Design and synthesis of novel antibacterial peptide-resin conjugates (2007) Bioorganic and Medicinal Chemistry Letters, 17 (21), pp. 5772-5776. , DOI 10.1016/j.bmcl.2007.08.056, PII S0960894X0701013X; 
Statz, A.R., Park, J.P., Chongsiriwatana, N.P., Barron, A.E., Messersmith, P.B., Surface-immobilised antimicrobial peptoids (2008) Biofouling, 24 (6), pp. 439-448; 
Deslouches, B., Gonzalez, I.A., DeAlmeida, D., Islam, K., Steele, C., Montelaro, R.C., Mietzner, T.A., De novo-derived cationic antimicrobial peptide activity in a murine model of Pseudomonas aeruginosa bacteraemia (2007) Journal of Antimicrobial Chemotherapy, 60 (3), pp. 669-672. , DOI 10.1093/jac/dkm253; 
Novak, K.F., Diamond, W.J., Kirakodu, S., Peyyala, R., Anderson, K.W., Montelaro, R.C., Mietzner, T.A., Efficacy of the de novo-derived antimicrobial peptide WLBU2 against oral bacteria (2007) Antimicrobial Agents and Chemotherapy, 51 (5), pp. 1837-1839. , DOI 10.1128/AAC.00924-06; 
Deslouches, B., Islam, K., Craigo, J.K., Paranjape, S.M., Montelaro, R.C., Mietzner, T.A., Activity of the de novo engineered antimicrobial peptide WLBU2 against Pseudomonas aeruginosa in human serum and whole blood: Implications for systemic applications (2005) Antimicrobial Agents and Chemotherapy, 49 (8), pp. 3208-3216. , DOI 10.1128/AAC.49.8.3208-3216.2005; 
Deslouches, B., Phadke, S.M., Lazarevic, V., Cascio, M., Islam, K., Montelaro, R.C., Mietzner, T.A., De novo generation of cationic antimicrobial peptides: Influence of length and tryptophan substitution on antimicrobial activity (2005) Antimicrobial Agents and Chemotherapy, 49 (1), pp. 316-322. , DOI 10.1128/AAC.49.1.316-322.2005; 
Levin, M., Quint, P.A., Goldstein, B., Barton, P., Bradley, J.S., Shemie, S.D., Recombinant bactericidal/permeability-increasing protein (rBPI21) as adjunctive treatment for children with severe meningococcal sepsis: A randomised trial. rBPI21 Meningococcal Sepsis Study Group (2000) Lancet, 356 (9234), pp. 961-967; 
Giroir, B.P., Scannon, P.J., Levin, M., Bactericidal/permeability-increasing protein-lessons learned from the phase III, randomized, clinical trial of rBPI21 for adjunctive treatment of children with severe meningococcemia (2001) Crit Care Med, 29 (7 SUPPL.), pp. 130-135; 
Brogden, K.A., Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? (2005) Nature Reviews Microbiology, 3 (3), pp. 238-250. , DOI 10.1038/nrmicro1098; 
Bastos, M., Bai, G., Gomes, P., Andreu, D., Goormaghtigh, E., Prieto, M., Energetics and partition of two cecropin-melittin hybrid peptides to model membranes of different composition (2008) Biophys J, 94 (6), pp. 2128-2141; 
Melo, M.N., Ferre, R., Castanho, M.A., Antimicrobial peptides: Linking partition, activity and high membrane-bound concentrations (2009) Nat Rev Microbiol, 7 (3), pp. 245-250; 
Haynie, S.L., Crum, G.A., Doele, B.A., Antimicrobial activities of amphiphilic peptides covalently bonded to a water-insoluble resin (1995) Antimicrob Agents Chemother, 39 (2), pp. 301-307; 
Humblot, V., Yala, J.F., Thebault, P., Boukerma, K., Hequet, A., Berjeaud, J.M., The antibacterial activity of Magainin i immobilized onto mixed thiols self-assembled monolayers (2009) Biomaterials, 30 (21), pp. 3503-3512; 
Bagheri, M., Beyermann, M., Dathe, M., Immobilization reduces the activity of surface-bound cationic antimicrobial peptides with no influence upon the activity spectrum (2009) Antimicrob Agents Chemother, 53 (3), pp. 1132-1141; 
Gabriel, M., Nazmi, K., Veerman, E.C., Nieuw Amerongen, A.V., Zentner, A., Preparation of LL-37-grafted titanium surfaces with bactericidal activity (2006) Bioconj Chem, 17 (2), pp. 548-550; 
Hilpert, K., Elliott, M., Jenssen, H., Kindrachuk, J., Fjell, C.D., Korner, J., Screening and characterization of surface-tethered cationic peptides for antimicrobial activity (2009) Chem Biol, 16 (1), pp. 58-69; 
Batista, M.K.S., Gallemi, M., Adeva, A., Gomes, C.A.R., Gomes, P., Facile regioselective synthesis of a novel chitosan-pexiganan conjugate with potential interest for the treatment of infected skin lesions (2009) Synthetic Commun, 39 (7), pp. 1228-1240; 
Venter, J.C., Immobilized and insolubilized drugs, hormones, and neurotransmitters: Properties, mechanisms of action and applications (1982) Pharmacological Reviews, 34 (2), pp. 153-187; 
Endo, Y., Tani, T., Kodama, M., Antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber (1987) Appl Environ Microbiol, 53 (9), pp. 2050-2055; 
Chuang, H.F., Shukla, A., Loose, C.R., Hammond-Cunningham, P.T., Stephanopoulos, G., (2009) Structures Including Antimicrobial Peptides, , United States; 
Chen, R., Cole, N., Willcox, M.D., Park, J., Rasul, R., Carter, E., Synthesis, characterization and in vitro activity of a surface-attached antimicrobial cationic peptide (2009) Biofouling, 25 (6), pp. 517-524; 
Steven, M.D., Hotchkiss, J.H., Covalent immobilization of an antimicrobial peptide on poly(ethylene) film (2008) J Appl Polym Sci, 110 (5), pp. 2665-2670; 
Appendini, P., Hotchkiss, J.H., Surface modification of poly(styrene) by the attachment of an antimicrobial peptide (2001) J Appl Polym Sci, 81 (3), pp. 609-616; 
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) International Journal of Peptide and Protein Research, 35 (3), pp. 161-214; 
Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B., A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes (2002) Angewandte Chemie - International Edition, 41 (14), pp. 2596-2599. , DOI 10.1002/1521-3773(20020715)41: 14<2596::AID-ANIE2596>3.0.CO;2-4; 
Abad, J.M., Velez, M., Santamaria, C., Guisan, J.M., Matheus, P.R., Vazquez, L., Gazaryan, I., Fernandez, V.M., Immobilization of peroxidase glycoprotein on gold electrodes modified with mixed epoxy-boronic acid monolayers (2002) Journal of the American Chemical Society, 124 (43), pp. 12845-12853. , DOI 10.1021/ja026658p; 
Lee, M.-R., Shin, I., Fabrication of chemical microarrays by efficient immobilization of hydrazide-linked substances on epoxide-coated glass surfaces (2005) Angewandte Chemie - International Edition, 44 (19), pp. 2881-2884. , DOI 10.1002/anie.200462720; 
Hudalla, G.A., Murphy, W.L., Immobilization of peptides with distinct biological activities onto stem cell culture substrates using orthogonal chemistries (2010) Langmuir, 26 (9), pp. 6449-6456; 
Cole, N., Hume, E.B., Vijay, A.K., Sankaridurg, P., Kumar, N., Willcox, M.D., In vivo performance of melimine as an antimicrobial coating for contact lenses in models of CLARE and CLPU (2010) Invest Ophthalmol Vis Sci, 51 (1), pp. 390-395
},
	month = {2011///},
	pages = {1431 - 1440},
	abstract = {Bacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs{\textquoteright} potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and long-term stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices. {\textcopyright} 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Amino Acid Sequence, amoxicillin, ampicillin, Animals, antibiotic agent, antibiotic resistance, Antimicrobial Cationic Peptides, Antimicrobial peptides, Bacteria (microorganisms), Bacterial adhesion, bacterial survival, Biocompatible Materials, biofilm, Biofilms, biomaterial, carbenicillin, cefazolin, Coated Materials, Biocompatible, Cytotoxicity, drug activity, drug half life, drug solubility, drug stability, drug targeting, gentamicin, human, Humans, Immobilized Proteins, Molecular Sequence Data, mouth inflammation, Nanostructured surfaces, nonhuman, penicillin G, polypeptide antibiotic agent, priority journal, review, sepsis, skin infection, soft tissue infection, Surface functionalization, Surface Properties, teicoplanin, tobramycin, vancomycin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79952187401\&partnerID=40\&md5=70d486a4c6e1ba88f804c0f0d4f4e510},
	author = {Costa, F. and Carvalho, I.F. and Montelaro, R.C. and Gomes, P. and Martins, M.C.L.}
}
@article {2294,
	title = {Prediction of neonatal acidemia by computer analysis of fetal heart rate and ST event signals},
	journal = {American Journal of Obstetrics and GynecologyAm. J. Obstet. Gynecol.},
	volume = {201},
	year = {2009},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: AJOGAdoi: 10.1016/j.ajog.2009.04.033PubMed ID: 19539895Language of Original Document: EnglishCorrespondence Address: Costa, A.; Departamento de Ginecologia e Obstetr{\'\i}cia, Faculdade de Medicina, Hospital S. Jo{\~a}oPortugal; email: cosantonia@gmail.comReferences: (2006) Perinatal Mortality Surveillance 2004: England, Wales, and Northern Ireland, , CEMACH (Confidential Enquiry into Maternal and Child Health), Confidential Enquiry into Maternal and Child Health, London, UK;(1997) 4th annual report, , CESDI (Confidential Enquiry into Stillbirths and Deaths in Infancy), Maternal and Child Health Research Consortium, London, UK; 
Ayres-de-Campos, D., Bernardes, J., Costa-Pereira, A., Pereira-Leite, L., Inconsistencies in classification by experts of cardiotocograms and subsequent clinical decision (1999) Br J Obstet Gynaecol, 106, pp. 1307-1310; 
Paneth, N., Bommarito, M., Stricker, J., Electronic fetal monitoring and later outcome (1993) Clin Invest Med, 16, pp. 159-165; 
Bernardes, J., Costa-Pereira, A., Ayres-de-Campos, D., van Geijn, H.P., Pereira-Leite, L., Evaluation of interobserver agreement of cardiotocograms (1997) Int J Gynecol Obstet, 57, pp. 33-37; 
Donker, D.K., van Geijn, H.P., Hasman, A., Interobserver variation in the assessment of fetal heart rate recordings (1993) Eur J Obstet Gynecol Reprod Biol, 52, pp. 21-28; 
Neilson, J.P., Fetal electrocardiogram (ECG) for fetal monitoring during labour (2006) Cochrane Database Syst Rev, 3; 
Doria, V., Papageorghiou, A., Gustafsson, A., Ugwumadu, A., Farrer, K., Arulkumaran, S., Review of the first 1502 cases of ECG-ST waveform analysis during labour in a teaching hospital (2007) BJOG, 114, pp. 1202-1207; 
Westerhuis, M., Kwee, A., van Ginkel, A., Drogtrop, A., Gyselaers, W., Visser, G., Limitations of ST analysis in clinical practice: three cases of intrapartum metabolic acidosis (2007) BJOG, 114, pp. 1194-1201; 
Amer-Wahlin, I., Arulkumaran, S., Hagberg, H., Marsal, K., Visser, G., Fetal electrocardiogram: ST waveform analysis in intrapartum surveillance (2007) BJOG, 114, pp. 1191-1193; 
Dawes, G.S., Redman, C.W., Smith, J.H., Improvements in the registration and analysis of fetal heart rate records at the bedside (1985) Br J Obstet Gynaecol, 92, pp. 317-325; 
Ayres-de-Campos, D., Costa-Santos, C., Bernardes, J., Prediction of neonatal state by computer analysis of fetal heart rate tracings: the antepartum arm of the SisPorto multicentre validation study (2005) Eur J Obstet Gynecol Reprod Biol, 118, pp. 52-60. , SisPorto Multicentre Validation Study Group; 
Dawes, G.S., Moulden, M., Redman, C.W.G., System 8000: computerized antenatal FHR analysis (1991) J Perinat Med, 19, pp. 47-51; 
Bernardes, J., Moura, C., Marques-de-S{\'a}, J.P., Pereira-Leite, L., The Porto system for automated cardiotocographic signal analysis (1991) J Perinat Med, 19, pp. 61-65; 
Ayres-de-Campos, D., Bernardes, J., Garrido, A., Marques-de-S{\'a}, J.P., Pereira-Leite, L., SisPorto 2.0-a program for automated analysis of cardiotocograms (2000) J Matern Fetal Med, 9, pp. 311-318; 
Ayres-de-Campos, D., Sousa, P., Costa, A., Bernardes, J., Omniview-SisPorto{\textregistered} 3.5-a central fetal monitoring station with online alerts based on computerized cardiotocogram+S.T.event analysis (2008) J Perinat Med, 36, pp. 260-264; 
Ayres-de-Campos, D., Bernardes, J., Kwee, A., Westehuis, M., Visser, G.H.A., Computer quantification of short-term variability as an adjunct to fetal electrocardiographic monitoring (2007) BJOG, 114, pp. 1446-1447; 
Downing, J.W., Houlton, P.C., Barclay, A., Extradural analgesia for cesarean section: a comparison with general anesthesia (1979) Br J Anaesth, 51, pp. 367-374; 
Crawford, J.S., James, F.M., Davies, P., Crawley, M., A further study of general anaesthesia for cesarean section (1976) Br J Anaesth, 48, pp. 661-667; 
Huch, A., Huch, C., Rooth, G., Guidelines for blood sampling and measurement of pH and blood gas values in obstetrics (1994) Eur J Obstet Gynecol Reprod Biol, 54, pp. 165-175; 
ACOG technical bulletin. Umbilical artery blood acid-base analysis (1996) Int J Gynecol Obstet, 52, pp. 305-310; 
Westgate, J., Garibaldi, J.M., Greene, K.R., Umbilical cord blood gas analysis at delivery: a time for quality data (1994) Br J Obstet Gynaecol, 101, pp. 1054-1063; 
Low, J.A., Intrapartum fetal asphyxia: definition, diagnosis and classification (1997) Am J Obstet Gynecol, 176, pp. 957-959; 
Fleiss, J.L., (1981) Statistical methods for rates and proportions. 2nd ed., , John Wiley \& Sons, New York, NY Section 5.6; 
Keith, R.D., Beckley, S., Garibaldi, J.M., Westgate, J.A., Ifeachor, E.C., Greene, K.R., A multicentre comparative study of 17 experts and an intelligent computer system for managing labour using cardiotocogram (1995) Br J Obstet Gynecol, 102, pp. 668-700; 
Chung, T.K., Mohajer, M.P., Yang, Z.j., Chang, A.M., Sahota, D.S., The prediction of fetal acidosis at birth by computerised analysis of intrapartum cardiotocography (1995) Br J Obstet Gynaecol, 102, pp. 454-460; 
Nielsen, P.V., Stigsby, B., Nickelsen, C., Nim, J., Computer assessment of the intrapartum cardiotocogram. II. The value of computer assessment compared with visual assessment (1988) Acta Obstet Gynecol Scand, 67, pp. 461-464; 
Policy statement. The Apgar score-American Academy of Pediatrics and American College of Obstetricians and Gynecologists (2006) Pediatrics, 117, pp. 1444-1447; 
Casey, B.M., McIntire, D.D., Leveno, K.J., The continuing value of the Apgar score for the assessment of the newborn infants (2001) N Engl J Med, 334, pp. 467-471; 
Moster, D., Lie, R.T., Irgens, L.M., Bjerkedal, T., Markestad, The association of Apgar score with subsequent death and cerebral palsy: a population-based study in term infants (2001) J Pediatr, 138, pp. 798-803
},
	month = {2009///},
	pages = {464.e1 - 464.e6},
	abstract = {Objective: The objective of the study was to evaluate the accuracy of computer analysis of fetal heart rate (FHR) and ST event signals in prediction of neonatal acidemia. Study Design: One hundred forty-eight FHR tracings were evaluated to identify red alerts provided by the system, based on automated analysis of FHR and ST event signals, and compared with the occurrence of umbilical artery acidemia (pH <=7.05). Results: The presence of red alerts obtained sensitivity of 1.00 (95\% confidence interval [CI], 0.56-1.00), specificity of 0.94 (95\% CI, 0.89-0.97), positive predictive value (PPV) of 0.47 (95\% CI, 0.22-0.72), negative predictive value (NPV) of 1 (95\% CI, 0.96-1.00), positive likelihood ratio (PLR) of 17.6 (95\% CI, 9.0-34.5), and negative likelihood ratio (NLR) of 0. When limiting analysis to red alerts that did not include ST data, sensitivity was 0.57 (95\% CI, 0.20-0.88), specificity was 0.97 (95\% CI, 0.92-0.99), PPV was 0.50 (95\% CI, 0.17-0.82), NPV was 0.98 (95\% CI, 0.93-0.99), PLR was 20.14 (95\% CI, 6.3-64.2), and NLR was 0.44 (95\% CI, 0.19-1.04). Conclusion: Computer analysis of FHR and ST event signals provide higher accuracy in predicting neonatal academia. {\textcopyright} 2009 Mosby, Inc. All rights reserved.},
	keywords = {acidemia, Acidosis, article, autoanalysis, Cardiotocography, computer analysis, computer assisted, computer prediction, Diagnosis, Computer-Assisted, diagnostic value, Electrocardiography, Female, Fetal heart rate, Fetal hypoxia, Fetal monitoring, fetus electrocardiography, fetus heart rate, Heart Rate, Fetal, human, Humans, Infant, Newborn, major clinical study, male, newborn, newborn acidosis, observational study, pH, Predictive Value of Tests, priority journal, Prospective Studies, prospective study, Reproducibility of Results, sensitivity and specificity, signal transduction, ST segment, umbilical artery},
	isbn = {00029378 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70350572323\&partnerID=40\&md5=6ffddbacf1381ab65c9bd35f8db108dd},
	author = {Costa, A. and Ayres-de-Campos, D. and Costa, F. and Santos, C. and Bernardes, J.}
}
@article {2304,
	title = {Skin adhesive versus subcuticular suture for perineal skin repair after episiotomy - A randomized controlled trial},
	journal = {Acta Obstetricia et Gynecologica ScandinavicaActa Obstet. Gynecol. Scand.},
	volume = {88},
	year = {2009},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: AOGSAdoi: 10.1080/00016340902883133Language of Original Document: EnglishCorrespondence Address: Mota, R.; Servi{\c c}o de Ginecologia e Obstetr{\'\i}cia, Hospital de S. Jo{\~a}o, 4200-319 Porto, Portugal; email: sisporto@med.up.ptChemicals/CAS: Tissue Adhesives; cyanoacrylic acid octyl ester, 6701-17-3; polyglactin, 26780-50-7, 34346-01-5Tradenames: Dermabond, EthiconManufacturers: EthiconReferences: Kettle, C., Hills, R.K., Jones, P., Darby, L., Gray, R., Johanson, R., Continuous versus interrupted perineal repair with standard or rapidly absorbed sutures after spontaneous vaginal birth: A randomized controlled trial (2002) Lancet, 359, pp. 2217-2223;Gordon, B., Mackrodt, C., Fern, E., Truesdale, A., Ayers, S., Grant, A., The Ipswich Childbirth Study: 1. A randomized evaluation of two stage postpartum perineal repair leaving the skin unsutured (1998) Br J Obstet Gynaecol, 105, pp. 435-440; 
Mackrodt, C., Gordon, B., Fern, E., Ayers, S., Truesdale, A., Grant, A., The Ipswich Childbirth Study: 2. A randomized comparison of polyglactin 910 with chromic catgut for postpartum perineal repair (1998) Br J Obstet Gynaecol, 105, pp. 441-445; 
Kettle, C., Johanson, R.B., Continuous versus interrupted sutures for perineal repair (2000) Cochrane DataBase Syst Rev, (2). , CD000947; 
Kettle, C., Hills, R.K., Ismail, K.M.K., Continuous versus interrupted sutures for repair of episiotomy or second degree tears (2007) Cochrane DataBase Syst Rev, (4). , CD000947; 
Gemynthe, A., Langhoff-Roos, J., Sahl, S., Knudsen, J., New VICRYL formulation: An improved method of perineal repair? (1996) Br J Midwifery, 4, pp. 230-234; 
Oboro, V.O., Tabowei, T.O., Loto, O.M., Bosah, J.O., A multicentre evaluation of the two-layered repair of postpartum perineal trauma (2003) J Obstet Gynaecol, 23, pp. 5-8; 
Kindberg, S., Stehouwer, M., Hvidman, L., Henriksen, T.B., Postpartum perineal repair performed by midwives: A randomised trial comparing two suture techniques leaving the skin unsutured (2008) Br J Obstet Gynaecol, 115, pp. 472-479; 
Gennari, R., Rotmensz, N., Ballardini, B., Scevola, S., Perego, E., Zanini, V., A prospective, randomized, controlled clinical trial of tissue adhesive (2-octylcyanoacrylate) versus standard wound closure in breast surgery (2004) Surgery, 136, pp. 593-599; 
Bernard, L., Doyle, J., Friedlander, S.F., Eichenfield, L.F., Gibbs, N.F., Cunningham, B.B., A prospective comparison of octylcya-noacrylate tissue adhesive (dermabond) and suture for the closure of excisional wounds in children and adolescents (2001) Arch Dermatol, 137, pp. 1177-1180; 
Matin, S.F., Prospective randomized trial of skin adhesive versus sutures for closure of 217 laparoscopic port-site incisions (2003) J Am Coll Surg, 196, pp. 845-853; 
Bhende, S., Rothenburger, S., Spangler, D.J., Dito, M., In vitro assessment of microbial barrier properties of Dermabond( topical skin adhesive (2002) Surg Infect, 3, pp. 251-257; 
Rogerson, L., Mason, G.C., Roberts, A.C., Preliminary experience with twenty perineal repairs using Indermil tissue adhesive (2000) Eur J Obstet Gynecol Reprod Biol, 88, pp. 139-142; 
Bowen, M.L., Selinger, M., Episiotomy closure comparing enbucrilate tissue adhesive with conventional sutures (2002) Int J Gynecol Obstet, 78, pp. 201-205; 
Adoni, A., Anteby, E., The use of Histoacryl for the episiotomy repair (1991) Br J Obstet Gynaecol, 98, pp. 476-478; 
Man, J., Quinn, J., Cyanoacrylate tissue adhesives (1997) Am J Cosm Surg, 14, pp. 413-416; 
Maw, J.L., Quinn, J.V., Wells, G.A., Ducic, Y., Odell, P.F., Lamothe, A., A prospective comparison of octylcyanoacrylate tissue adhesive and suture for the closure of head and neck incisions (1997) J Otolaryngol, 26, pp. 26-30
},
	month = {2009///},
	pages = {660 - 666},
	abstract = {Objective. Surgical repair of perineal lesions after delivery is frequently associated with pain and discomfort, interfering with the normal activities of the puerperium. The aim of this study was to compare perineal skin repair after episiotomy with adhesive glue versus a subcuticular suture, regarding the incidence of pain and wound complications. Study design. Randomized clinical trial. Setting. Tertiary care university hospital. Material and methods. One hundred women having mediolateral episiotomy at vaginal delivery were enrolled. They were randomized to receive skin adhesive (n = 53) or subcuticular suture (n = 47) for closure of perineal skin. The main outcome measure was self-evaluated pain in the 30 days following delivery. Secondary outcome measures were technical difficulties reported with the procedure, duration of surgical repair, wound complications observed at 42-68 hours post-partum and re-initiation of sexual activity by 30 days post-partum. Results. No significant differences were observed between the two groups in incidence of technical difficulties and failed procedures, pain during the procedure, wound complications at hospital discharge, self-evaluated measures of pain at 7 and 30 days or re-initiation of sexual activity by 30 days post-partum. The skin adhesive group had a significantly shorter mean duration of the procedure (four minutes less). Conclusion. Perineal skin closure using adhesive glue is faster than subcuticular suture, and associated with a similar incidence of complications and pain in the first 30 days.},
	keywords = {adult, article, clinical trial, comparative study, controlled clinical trial, controlled study, cyanoacrylic acid octyl ester, edema, episiotomy, Episiotomy closure, Female, granuloma, human, Humans, injury, major clinical study, obstetric care, pain, pain assessment, Perineal repair, perineum, perineum injury, polyglactin, priority journal, questionnaire, randomized controlled trial, sexual intercourse, Skin, Skin adhesive, suture, Suture Techniques, suturing method, Tissue adhesive, Tissue Adhesives, treatment outcome, vaginal delivery, wound closure, wound dehiscence, Wounds and Injuries},
	isbn = {00016349 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-66149113694\&partnerID=40\&md5=2a28b4b09b79b1c79c4cf903af92a16c},
	author = {Mota, R. and Costa, F. and Amaral, A. and Oliveira, F. and Santos, C.C. and Ayres-de-Campos, D.}
}
