



@article {2231,
	title = {Molecular recognition force spectroscopy: A new tool to tailor targeted nanoparticles},
	journal = {SmallSmall},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1002/smll.201002074Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB-Instituto de Engenharia Biom{\'e}dica, Divis̃o de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptReferences: Verma, I.M., Weitzman, M.D., (2005) Annu. Rev. Biochem., 74, pp. 711-738;Midoux, P., Breuzard, G., Gomez, J.P., Pichon, C., (2008) Curr. Gene Ther., 8, pp. 335-352; 
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},
	month = {2011///},
	pages = {1236 - 1241},
	abstract = {The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine. A novel screening tool is described to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. The unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. {\textcopyright} 2011 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.},
	keywords = {article, Atomic force microscopy, Atomic spectroscopy, atomic-force microscopy, chemistry, Event probability, Force spectroscopy, Functionalized nanoparticles, gene technology, Gene-delivery systems, Genes, Medical nanotechnology, medicine, methodology, Microscopy, Atomic Force, Molecular biology, Molecular recognition, molecular recognition force spectroscopy, Nanomedicines, nanoparticle, Nanoparticle formulation, nanoparticles, Nanotechnology, Neuronal cell, Optimization, Receptor interaction, Screening tool, Specific interaction, Targeted nanoparticle},
	isbn = {16136810 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955561627\&partnerID=40\&md5=85fa523edfcd7a6e171a53820be7f230},
	author = {Oliveira, H. and Rangl, M. and Ebner, A. and Mayer, B. and Hinterdorfer, P. and P{\^e}go, A.P.}
}
