



@article {2236,
	title = {Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2010.11.005PubMed ID: 21056701Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; carbenicillin, 17230-86-3, 4697-36-3, 4800-94-6; cefazolin, 25953-19-9, 27164-46-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; penicillin G, 1406-05-9, 61-33-6; teicoplanin, 61036-62-2, 61036-64-4; tobramycin, 32986-56-4; vancomycin, 1404-90-6, 1404-93-9; Antimicrobial Cationic Peptides; Biocompatible Materials; Coated Materials, Biocompatible; Immobilized ProteinsReferences: Glinel, K., Jonas, A.M., Jouenne, T., Leprince, J., Galas, L., Huck, W.T., Antibacterial and antifouling polymer brushes incorporating antimicrobial peptide (2009) Bioconj Chem, 20 (1), pp. 71-77;Vila, J., Soriano, A., Mensa, J., Molecular basis of microbial adherence to prosthetic materials. Role of biofilms in prosthesis-associated infection (2008) Enferm Infecc Microbiol Clin, 26 (1), pp. 48-54; 
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},
	month = {2011///},
	pages = {1431 - 1440},
	abstract = {Bacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs{\textquoteright} potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and long-term stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices. {\textcopyright} 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Amino Acid Sequence, amoxicillin, ampicillin, Animals, antibiotic agent, antibiotic resistance, Antimicrobial Cationic Peptides, Antimicrobial peptides, Bacteria (microorganisms), Bacterial adhesion, bacterial survival, Biocompatible Materials, biofilm, Biofilms, biomaterial, carbenicillin, cefazolin, Coated Materials, Biocompatible, Cytotoxicity, drug activity, drug half life, drug solubility, drug stability, drug targeting, gentamicin, human, Humans, Immobilized Proteins, Molecular Sequence Data, mouth inflammation, Nanostructured surfaces, nonhuman, penicillin G, polypeptide antibiotic agent, priority journal, review, sepsis, skin infection, soft tissue infection, Surface functionalization, Surface Properties, teicoplanin, tobramycin, vancomycin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79952187401\&partnerID=40\&md5=70d486a4c6e1ba88f804c0f0d4f4e510},
	author = {Costa, F. and Carvalho, I.F. and Montelaro, R.C. and Gomes, P. and Martins, M.C.L.}
}
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},
	month = {2008///},
	pages = {1775 - 1785},
	abstract = {This work consists on the evaluation of the in vitro performance of Ti6Al4V samples PLD (pulsed laser deposition) coated with hydroxyapatite, both pure and mixed with a CaO-P                        2O
                        5 glass. Previous studies on immersion of PLD coatings in SBF, showed that the immersion apatite films did not present the usual cauliflower morphology but replicated the original columnar structure and exhibited good bioactivity. However, the influence of glass associated to hydroxyapatite concerning adhesion, proliferation and morphology of MG63 cells on the films surface was unclear. In this study, the performance of these PLD coated samples was evaluated, not only following the physical-chemical transformations resulting from the SBF immersion, but also evaluating the cytocompatibility in contact with osteoblast-like MG63 cells. SEM and AFM confirmed that the bioactive ceramic PLD films reproduce the substrate{\textquoteright}s surface topography and that the films presented good adherence and uniform surface roughness. Physical-chemical phenomena occurring during immersion in SBF did not modify the original columnar structure. In contact with MG63 cells, coated samples exhibited very good acceptance and cytocompatibility when compared to control. The glass mixed with hydroxyapatite induced higher cellular proliferation. Cells grown on these samples presented many filipodia and granular structures, typical features of osteoblasts. {\textcopyright} 2007 Springer Science+Business Media, LLC.
},
	keywords = {Apatite, Apatite films, Bioactive ceramic films, Bioactive glass, Bioactivity, Bioceramics, Biocompatible Materials, Biofilms, Brassica oleracea var. botrytis, Calcium Compounds, calcium oxide, Cauliflower morphology, cell adhesion, cell proliferation, cell structure, cell survival, ceramics, Columnar structure, conference paper, controlled study, Durapatite, glass, human, human cell, Humans, hydroxyapatite, Lasers, materials testing, Microscopy, Atomic Force, Microscopy, Electron, Scanning, morphology, osteoblast, Osteoblasts, oxide, Oxides, phosphorous pentoxide, Phosphorus Compounds, Polystyrenes, priority journal, Pulsed laser deposition, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-40949140642\&partnerID=40\&md5=de09d5133a0616668cb5e57135d91ae6},
	author = {Oliveira, G.M. and Ferraz, M.P. and Gonz{\'a}lez, P.G. and Serra, J. and Leon, B. and P{\`e}rez-Amor, M. and Monteiro, F.J.}
}
@article {2381,
	title = {Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation},
	journal = {Journal of Biomaterials Science, Polymer EditionJ. Biomater. Sci. Polym. Ed.},
	volume = {18},
	year = {2007},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBSEEdoi: 10.1163/156856207780425068Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; Biocompatible Materials; Chitosan, 9012-76-4References: Vacanti, C.A., Bonassar, L.J., Vacanti, J.P., (2000) Principles of Tissue Engineering, p. 671. , R. P. Lanza, R. Langer and J. Vacanti Eds, p, Academic Press, San Diego, CA;Bruder, S.P., Caplan, A.I., (2000) Principles of Tissue Engineering, p. 683. , R. P. Lanza, R. Langer and J. Vacanti Eds, p, Academic Press, San Diego, CA; 
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},
	month = {2007///},
	pages = {469 - 485},
	abstract = {Chitosan (Ch) is being actively investigated as a non-protein template for the growth of an increasing number of anchorage-dependent cells, including chondrocytes and bone cells. In the present work, Ch films with degrees of N-acetylation (DAs) in the range of 4 to 49\% were evaluated with respect to the attachment, spreading and short-term proliferation of osteoblasts, using human osteoblastic MG-63 cells. The films were characterized in terms of surface morphology and surface charge by atomic force microscopy and streaming potential measurements, respectively. Cell attachment was assessed after 3 and 24 h of cell culture. After 24 h of incubation, cell attachment was found to be dependent on the DA, lower DAs favouring cell adhesion. With time, cell spreading and cytoskeleton organization were only attained for DAs <= 13\%. Regarding cell proliferation, cells grown on films with the lowest DA (4\%) revealed a higher specific growth rate as compared to those grown on films with higher DAs. Films with a DA of 49\% failed to sustain cell proliferation. In addition, a longer lag-phase was observed on Ch, as compared to TCPS, in accordance to an observed delay of cell spreading. The present findings revealed that differences in the DA as small as 9\% may be critical in terms of the osteoblast response to two-dimensional Ch-based matrices. {\textcopyright} 2007 VSP.},
	keywords = {Acetylation, AFM, article, Atomic force microscopy, Biocompatible Materials, biofilm, Biofilms, biomaterial, bone cell, cartilage cell, cell adhesion, cell anchorage, Cell culture, cell growth, Cell Line, Tumor, cell proliferation, Cell Shape, cell spreading, cell survival, Chemical structure, chemistry, Chitin, chitosan, comparative study, controlled study, cytoskeleton, Degree of acetylation, drug effect, Growth kinetics, human, human cell, Humans, incubation time, metabolism, Microscopy, Atomic Force, osteoblast, Osteoblast-like cells, Osteoblasts, Pathology, priority journal, Surface charge, Surface Properties, surface property, Surface treatment, tumor cell line, Zeta potential},
	isbn = {09205063 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34347375992\&partnerID=40\&md5=437b9ed270e859425b76aa3655c611be},
	author = {Amaral, I.F. and Cordeiro, A.L. and Sampaio, P. and Barbosa, M.A.}
}
@book {2385,
	title = {Characterization of hydroxyapatite sputtered films doped with titanium},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 I},
	year = {2007},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ribeiro, A.R.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: arr@ineb.up.ptReferences: Bhat, (2002) Biomaterials, , Narosa Publishing House/Kluwer Academic Publishers, India;Feddes B, Vredenberg AM. Wolke JGC, Jansen JA.: Surface and Coatings Technology 185 (2-3) (2004), p. 346Gisep, A., (2002) Injury-International Journal of the Care of the Injured, 33 (SUPPL. 2), p. 88; 
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},
	month = {2007///},
	pages = {649 - 652},
	abstract = {In the present work, thin films of hydroxyapatite (HAp), titanium, and hydroxyapatite doped with different titanium concentrations were obtained by sputtering and characterized using several analytical techniques. These films are intended to be used as model surfaces on protein adsorption studies in order to better understand the role of titanium ions in biological processes.},
	keywords = {Adsorption, Biofilms, Doping (additives), hydroxyapatite, Hydroxyapatite sputtered films, Model surfaces, Proteins, Sputtering, Titanium, Titanium ions},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846300257\&partnerID=40\&md5=7946215d8f37e0e216b57336b89f0f02},
	author = {Ribeiro, A.R. and Piedade, A.P. and Ribeiro, C.C. and Vieira, M.T. and Barbosa, M.A.}
}
