



@article {2209,
	title = {Cell-based in vitro models for predicting drug permeability},
	journal = {Expert Opinion on Drug Metabolism and ToxicologyExpert Opin. Drug Metab. Toxicol.},
	volume = {8},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.1517/17425255.2012.673586PubMed ID: 22424145Language of Original Document: EnglishCorrespondence Address: Sarmento, B.; CICS, Department of Pharmaceutical Sciences, Instituto Superior de Ci{\^e}ncias da Sa{\'u}de - Norte, Gandra, Portugal; email: bruno.sarmento@ff.up.ptChemicals/CAS: Pharmaceutical PreparationsReferences: Polli, J., In vitro studies are sometimes better than conventional human pharmacokinetic in vivo studies in assessing bioequivalence of immediate-release solid oral dosage forms (2008) AAPS J., 10, pp. 289-299;Pelkonen, O., Boobis, A.R., Gundert-Remy, U., In vitro prediction of gastrointestinal absorption and bioavailability: An experts{\textquoteright} meeting report (2001) Eur. J. Clin. Pharmacol., 57, pp. 621-629; 
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Aukunuru, J.V., Sunkara, G., Ayalasomayajula, S.P., Deruiter, J., Clark, R.C., Kompella, U.B., A biodegradable injectable implant sustains systemic and ocular delivery of an aldose reductase inhibitor and ameliorates biochemical changes in a galactose-fed rat model for diabetic complications (2002) Pharmaceutical Research, 19 (3), pp. 278-285. , DOI 10.1023/A:1014438800893; 
Luthra, S., Narayanan, R., Marques, L.E.A., Chwa, M., Kim, D.W., Dong, J., Seigel, G.M., Kuppermann, B.D., Evaluation of in vitro effects of bevacizumab (Avastin) on retinal pigment epithelial, neurosensory retinal, and microvascular endothelial cells (2006) Retina, 26 (5), pp. 512-518. , DOI 10.1097/01.iae.0000222547.35820.52, PII 0000698220060500000003; 
Flamand, N., Marrot, L., Belaidi, J.-P., Bourouf, L., Dourille, E., Feltes, M., Meunier, J.-R., Development of genotoxicity test procedures with Episkin, a reconstructed human skin model: Towards new tools for in vitro risk assessment of dermally applied compounds? (2006) Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 606 (1-2), pp. 39-51. , DOI 10.1016/j.mrgentox.2006.02.009, PII S1383571806000593; 
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Netzlaff, F., Lehr, C.-M., Wertz, P.W., Schaefer, U.F., The human epidermis models EpiSkin, SkinEthic and EpiDerm An evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport (2005) European Journal of Pharmaceutics and Biopharmaceutics, 60 (2), pp. 167-178. , DOI 10.1016/j.ejpb.2005.03.004, PII S0939641105001116; 
Hu, T., Bailey, R.E., Morrall, S.W., Dermal penetration and metabolism of p-aminophenol and p-phenylenediamine: Application of the EpiDerm human reconstructed epidermis model (2009) Toxicol. Lett., 188, pp. 119-129; 
Gabbanini, S., Matera, R., Beltramini, C., Analysis of in vitro release through reconstructed human epidermis and synthetic membranes of multi-vitamins from cosmetic formulations (2010) J. Pharm. Biomed. Anal., 52, pp. 461-467; 
Balimane, P.V., Chong, S., Cell culture-based models for intestinal permeability: A critique (2005) Drug Discovery Today, 10 (5), pp. 335-343. , DOI 10.1016/S1359-6446(04)03354-9, PII S1359644604033549
},
	month = {2012///},
	pages = {607 - 621},
	abstract = {In vitro cell models have been used to predict drug permeation in early stages of drug development, since they represent an easy and reproducible method, allowing the tracking of drug absorption rate and mechanism, with an advantageous costbenefit ratio. Such cell-based models are mainly composed of immortalized cells with an intrinsic ability to grow in a monolayer when seeded in permeable supports, maintaining their physiologic characteristics regarding epithelium cell physiology and functionality. Areas covered: This review summarizes the most important intestinal, pulmonary, nasal, vaginal, rectal, ocular and skin cell-based in vitro models for predicting the permeability of drugs. Moreover, the similitude between in vitro cell models and in vivo conditions are discussed, providing evidence that each model may provisionally resemble different drug absorption route. Expert opinion: Despite the widespread use of in vitro cell models for drug permeability and absorption evaluation purposes, a detailed study on the properties of these models and their in vitroin vivo correlation compared with human data are required to further use in order to consider a future drug discovery optimization and clinical development. {\textcopyright} 2012 Informa UK, Ltd.},
	keywords = {Animals, Caco-2 Cells, carcinoma cell, cell differentiation, cell growth, Cell Line, Cell Membrane Permeability, cell models, cell proliferation, cell strain CACO 2, cell type, Cells, conjunctiva, drug absorption, drug bioavailability, drug delivery system, drug penetration, drug screening, drug transport, epithelium cell, Eye, human, Humans, in vitro models, in vitro study, in vitroin vivo correlation, Intestinal Absorption, intestinal mucosa, intestine cell, Intestines, Lung, lung alveolus cell, Models, Biological, molecular weight, nasal cell, nonhuman, ocular cell, permeability, Pharmaceutical Preparations, rectal cell, review, Skin, skin cell, vaginal cell},
	isbn = {17425255 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859967380\&partnerID=40\&md5=f531a0a6e96c96e8daf71a6b36dbec8a},
	author = {Sarmento, B. and Andrade, F. and Da Silva, S.B. and Rodrigues, F. and Das Neves, J. and Ferreira, D.}
}
@article {2194,
	title = {A model for educational simulation of the evolution of uterine contractions during labor},
	journal = {Computer Methods and Programs in BiomedicineComput. Methods Programs Biomed.},
	volume = {107},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: CMPBEdoi: 10.1016/j.cmpb.2011.09.016PubMed ID: 22047937Language of Original Document: EnglishCorrespondence Address: Bastos, L.F.; Campus da FEUP, Rua Dr. Roberto Frias, s/n, sala I305, 4200-465 Porto, Portugal; email: lbastos@fe.up.ptReferences: Curran, C.A., Intrapartum emergencies (2003) J. Obstet. Gynecol. Neonatal Nurs., 32 (6), pp. 802-813;Guise, J.-M., Anticipating and responding to obstetric emergencies (2007) Best Pract. Res. Clin. Obstet. Gynaecol., 21 (4), pp. 625-638; 
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},
	month = {2012///},
	pages = {242 - 247},
	abstract = {Electronic fetal monitoring remains an important tool in labor ward settings, providing continuous information on fetal heart rate and maternal uterine contractions. A prompt detection of abnormalities in these signals is essential for the timely resolution of situations that may put both mother and fetus at risk. Uterine contraction signals provide information that is important to evaluate the onset and progress of labor, as well as the significance of certain fetal heart rate abnormalities. We present a model for educational simulation of the spontaneous evolution of uterine contractions during labor, which combines a previously published signal generator with literature-based pre-programmed scripts for educationally relevant scenarios. This model is an essential component of a high-fidelity simulator of intrapartum emergencies, aimed to improve the competency of healthcare providers. Real and simulated tracings were presented to three independent clinical experts who judged simulated signals to be indistinguishable or negligibly different from real tracings. {\textcopyright} 2011 Elsevier Ireland Ltd.},
	keywords = {article, clinical competence, computer memory, computer program, Computer simulation, Computer-Assisted Instruction, controlled study, Educational simulations, Essential component, Female, Fetal heart rate, Fetal monitoring, Health care, Health care providers, High-fidelity simulators, Humans, labor, Labor and delivery, medical education, Medical educational simulation, Models, Biological, Neonatal monitoring, Obstetric Labor Complications, Obstetrics, Pregnancy, Script-controlled simulation, signal detection, Signal generators, Simulated signals, User-Computer Interface, Uterine activities, Uterine activity, Uterine Contraction, Uterine Monitoring, uterus contraction},
	isbn = {01692607 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861989435\&partnerID=40\&md5=6b5099845e4592661e2f426170d9f8b9},
	author = {Bastos, L.F. and van Meurs, W. and Ayres-de-Campos, D.}
}
@article {2300,
	title = {Automated Arabidopsis plant root cell segmentation based on SVM classification and region merging},
	journal = {Computers in Biology and MedicineComput. Biol. Med.},
	volume = {39},
	year = {2009},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: CBMDAdoi: 10.1016/j.compbiomed.2009.06.008PubMed ID: 19604506Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUP, Portugal; email: marcuzzo@fe.up.ptReferences: Baeza-Yates, R., Ribeiro-Neto, B., (1999) Modern Information Retrieval, , ACM Press;Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Computer Vision and Image Understanding, 77 (3), pp. 317-370; 
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Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem (2006) Plant Journal, 48, pp. 619-627; 
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},
	month = {2009///},
	pages = {785 - 793},
	abstract = {To obtain development information of individual plant cells, it is necessary to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Automation of cell detection/marking process is important to provide research tools in order to ease the search for special events, such as cell division. In this paper we discuss an automatic cell detection approach for Arabidopsis thaliana based on segmentation, which selects the best cell candidates from a starting watershed-based image segmentation and improves the result by merging adjacent regions. The selection of individual cells is obtained using a support vector machine (SVM) classifier, based on a cell descriptor constructed from the shape and edge strength of the cells{\textquoteright} contour. In addition we proposed a novel cell merging criterion based on edge strength along the line that connects adjacent cells{\textquoteright} centroids, which is a valuable tool in the reduction of cell over-segmentation. The result is largely pruned of badly segmented and over-segmented cells, thus facilitating the study of cells. When comparing the results after merging with the basic watershed segmentation, we obtain 1.5\% better coverage (increase in F-measure) and up to 27\% better precision in correct cell segmentation. {\textcopyright} 2009 Elsevier Ltd. All rights reserved.},
	keywords = {accuracy, Arabidopsis, Arabidopsis plant, Arabidopsis thaliana, article, artificial intelligence, automation, Bioinformatics, Biological cells, Biomedical image processing, Cell detection, Cell divisions, Cell membranes, Cell merging, cell proliferation, Cell segmentation, Cell Shape, Cell Wall, Computer simulation, confocal microscopy, controlled study, Descriptor, F-measure, Image analysis, image processing, Image Processing, Computer-Assisted, Image segmentation, Imaging systems, In-Vivo imaging, Individual cells, Landforms, Merging, Models, Biological, nonhuman, Over segmentation, Plant cells, plant root, Plant roots, Plants (botany), priority journal, Region-merging, Research tools, root morphology, Segmented cell, signal noise ratio, support vector machine, Support vector machines, SVM classification, Watershed segmentation, Watersheds},
	isbn = {00104825 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67849119398\&partnerID=40\&md5=8029bce67aff136ccc5e0f7e4f9e0ded},
	author = {Marcuzzo, M. and Quelhas, P. and Campilho, A. and Maria Mendon{\c c}a, A. and Campilho, A.}
}
@article {2322,
	title = {Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement},
	journal = {Proceedings of the National Academy of Sciences of the United States of AmericaProc. Natl. Acad. Sci. U. S. A.},
	volume = {106},
	year = {2009},
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Simmons, C.A., Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells (2004) Bone, 35, pp. 562-569; 
Comisar, W.A., Kazmers, N.H., Mooney, D.J., Linderman, J.J., Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: A combined computational and experimental approach (2007) Biomaterials, 28, pp. 4409-4417; 
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Richardson, T.P., Peters, M.C., Ennett, A.B., Mooney, D.J., Polymeric system for dual growth factor delivery (2001) Nat Biotechnol, 19, pp. 1029-1034
},
	month = {2009///},
	pages = {399 - 404},
	abstract = {Three-dimensional culture alters cancer cell signaling; however, the underlying mechanisms and importance of these changes on tumor vascularization remain unclear. A hydrogel system was used to examine the role of the transition from 2D to 3D culture, with and without integrin engagement, on cancer cell angiogenic capability. Three-dimensional culture recreated tumor microenvironmental cues and led to enhanced interleukin 8 (IL-8) secretion that depended on integrin engagement with adhesion peptides coupled to the polymer. In contrast, vascular endothelial growth factor (VEGF) secretion was unaffected by 3D culture with or without substrate adhesion. IL-8 diffused greater distances and was present in higher concentrations in the systemic circulation, relative to VEGF. Implantation of a polymeric IL-8 delivery system into GFP bone marrow-transplanted mice revealed that localized IL-8 up-regulation was critical to both the local and systemic control of tumor vascularization in vivo. In summary, 3D integrin engagement within tumor microenvironments regulates cancer cell angiogenic signaling, and controlled local and systemic blockade of both IL-8 and VEGF signaling may improve antiangiogenic therapies. {\textcopyright} 2009 by The National Academy of Sciences of the USA.},
	keywords = {animal experiment, animal model, Animals, arginylglycylaspartic acid, article, bone marrow transplantation, cancer cell, cancer cell culture, Cell Culture Techniques, concentration (parameters), controlled study, cytokine release, Diffusion, Drug delivery, ECM, Humans, hydrogel, Hydrogels, IL-8, implantation, in vivo study, integrin, Integrins, interleukin 8, Interleukin-8, Mice, microenvironment, Models, Biological, mouse, Mus, Neoplasms, Neovascularization, Pathologic, nonhuman, polymer, priority journal, signal transduction, systemic circulation, Tumor vascularization, upregulation, Vascular Endothelial Growth Factor A, vasculotropin},
	isbn = {00278424 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849121351\&partnerID=40\&md5=acc07042d6f5801f7b704a3f355917cf},
	author = {Fischbach, C. and Hyun, J.K. and Hsiong, S.X. and Evangelista, M.B. and Yuen, W. and Mooney, D.J.}
}
@article {2466,
	title = {A model for educational simulation of infant cardiovascular physiology},
	journal = {Anesthesia and AnalgesiaAnesth. Analg.},
	volume = {99},
	year = {2004},
	note = {Cited By (since 1996): 19Export Date: 21 November 2012Source: ScopusCODEN: AACRAdoi: 10.1213/01.ANE.0000134797.52793.AFPubMed ID: 15562049Language of Original Document: EnglishCorrespondence Address: Goodwin, J.A.; Nemours Children{\textquoteright}s C., 807 Children{\textquoteright}s Way, Jacksonville, FL 32207, United States; email: jgoodwin@nemours.orgReferences: Good, M.L., Gravenstein, J.S., Training for safety in an anesthesia simulator (1993) Semin Anesth, 12, pp. 235-250;Gaba, D.M., DeAnda, A., The response of anesthesia trainees to simulated critical incidents (1989) Anesth Analg, 68, pp. 444-451; 
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Halamek, L.P., Kaegi, D.M., Gaba, D.M., Time for a new paradigm in pediatric medical education: Teaching neonatal resuscitation in a simulated delivery room environment (2000) Pediatrics, 106, pp. E45; 
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},
	month = {2004///},
	pages = {1655 - 1664},
	abstract = {Full-body patient simulators provide the technology and the environment necessary for excellent clinical education while eliminating risk to the patient. The extension of simulator-based training into management of basic and critical situations in complex patient populations is natural. We describe the derivation of an infant cardiovascular model through the redefinition of a complete set of parameters for an existing adult model. Specifically, we document a stepwise parameter estimation process, explicit simplifying assumptions, and sources for these parameters. The simulated vital signs are within the target hemodynamic variables, and the simulated systemic arterial pressure wave form and left ventricular pressure volume loop are realistic. The system reacts appropriately to blood loss, and incorporation of aortic stenosis is straightforward. This infant cardiovascular model can form the basis for screen-based educational simulations. The model is also an essential step in attaining a full-body, model-driven infant simulator.},
	keywords = {aging, Algorithms, Anesthesiology, aorta stenosis, Aortic Valve Stenosis, arterial pressure, article, Baroreflex, biological model, bleeding, Blood Loss, Surgical, cardiovascular function, Cardiovascular physiology, Computer simulation, education, heart ventricle pressure, hemodynamic parameters, Hemodynamic Processes, Humans, Infant, Models, Biological, priority journal, Pulmonary Circulation, simulation, Ventricular Function, Left, Ventricular Function, Right},
	isbn = {00032999 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-9244231824\&partnerID=40\&md5=afb81c88d315abadfc3022fc3eba159a},
	author = {Goodwin, J.A. and van Meurs, W.L. and S{\'a} Couto, C.D. and Beneken, J.E.W. and Graves, S.A.}
}
