



@article {2210,
	title = {Tumor cell-educated periprostatic adipose tissue acquires an aggressive cancer-promoting secretory profile},
	journal = {Cellular Physiology and BiochemistryCell. Physiol. Biochem.},
	volume = {29},
	year = {2012},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: CEPBEdoi: 10.1159/000337604PubMed ID: 22415092Language of Original Document: EnglishCorrespondence Address: Ribeiro, R.J.T.; Portuguese Institute of Oncology, Porto Centre Molecular Oncology Group -CI, Edif{\'\i}cio Laborat{\'o}rios -Piso 4, Rua Dr. Ant{\'o}nio Bernardino Almeida, 4200-072 Porto, Portugal; email: oriebir.r@gmail.comChemicals/CAS: adiponectin, 283182-39-8; gelatinase B, 146480-36-6; osteopontin, 106441-73-0; Adiponectin; Culture Media, Conditioned; Cytokines; DNA, Mitochondrial; Interleukin-6; Matrix Metalloproteinase 2, 3.4.24.24; Matrix Metalloproteinase 9, 3.4.24.35; Osteopontin, 106441-73-0; Tumor Necrosis Factor-alphaReferences: Hsing, A.W., Sakoda, L.C., Chua Jr., S., Obesity, metabolic syndrome, and prostate cancer (2007) Am J Clin Nutr, 86, pp. s843-857;Cao, Y., Ma, J., Body mass index, prostate cancer-specific mortality, and biochemical recurrence: A systematic review and meta-analysis (2011) Cancer Prev Res (Phila), 4, pp. 486-501; 
Baillargeon, J., Rose, D.P., Obesity, adipokines, and prostate cancer (review) (2006) Int J Oncol, 28, pp. 737-745; 
Mistry, T., Digby, J.E., Desai, K.M., Randeva, H.S., Obesity and Prostate Cancer: A Role for Adipokines (2007) European Urology, 52 (1), pp. 46-53. , DOI 10.1016/j.eururo.2007.03.054, PII S0302283807004307; 
Hirano, T., Morii, H., Nakazawa, K., Murakami, D., Yamashita, A., Asahi, J., Orimo, H., Tokura, Y., Enhancement of adipogenesis induction by conditioned media obtained from cancer cells (2008) Cancer Lett, 268, pp. 286-294; 
Andarawewa, K.L., Motrescu, E.R., Chenard, M.-P., Gansmuller, A., Stoll, I., Tomasetto, C., Rio, M.-C., Stromelysin-3 is a potent negative regulator of adipogenesis participating to cancer cell-adipocyte interaction/crosstalk at the tumor invasive front (2005) Cancer Research, 65 (23), pp. 10862-10871. , DOI 10.1158/0008-5472.CAN-05-1231; 
Jotzu, C., Alt, E., Welte, G., Li, J., Hennessy, B.T., Devarajan, E., Krishnappa, S., Song, Y.H., Adipose tissue derived stem cells differentiate into carcinoma-associated fibroblast-like cells under the influence of tumor derived factors (2011) Cell Oncol (Dordr), 34, pp. 55-67; 
Dolinkova, M., Dostalova, I., Lacinova, Z., Michalsky, D., Haluzikova, D., Mraz, M., The endocrine profile of subcutaneous and visceral adipose tissue of obese patients (2008) Mol Cell Endocrinol, 291, pp. 63-70; 
Van Roermund, J.G., Hinnen, K.A., Tolman, C.J., Bol, G.H., Witjes, J.A., Bosch, J.L., Kiemeney, L.A., Van Vulpen, M., Periprostatic fat correlates with tumour aggressiveness in prostate cancer patients (2011) BJU Int, 107, pp. 1775-1779; 
Sumitomo, M., Asakuma, J., Yoshii, H., Sato, A., Horiguchi, A., Ito, K., Nagakura, K., Asano, T., Anterior perirectal fat tissue thickness is a strong predictor of recurrence after high-intensity focused ultrasound for prostate cancer (2010) Int J Urol, 17, pp. 776-782; 
Finley, D.S., Calvert, V.S., Inokuchi, J., Lau, A., Narula, N., Petricoin, E.F., Zaldivar, F., Ornstein, D.K., Periprostatic adipose tissue as a modulator of prostate cancer aggressiveness (2009) J Urol, 182, pp. 1621-1627; 
Thalmann, S., Juge-Aubry, C.E., Meier, C.A., Explant cultures of white adipose tissue (2008) Methods Mol Biol, 456, pp. 195-199; 
Song, G.J., Lewis, V., Mitochondrial DNA integrity and copy number in sperm from infertile men (2008) Fertil Steril, 90, pp. 2238-2244; 
Park, J., Euhus, D.M., Scherer, P.E., Paracrine and Endocrine Effects of Adipose Tissue on Cancer Development and Progression (2011) Endocr Rev, 32, pp. 550-570; 
George, D.J., Halabi, S., Shepard, T.F., Sanford, B., Vogelzang, N.J., Small, E.J., Kantoff, P.W., The prognostic significance of plasma interleukin-6 levels in patients with metastatic hormone-refractory prostate cancer: Results from cancer and leukemia group B 9480 (2005) Clinical Cancer Research, 11 (5), pp. 1815-1820. , DOI 10.1158/1078-0432.CCR-04-1560; 
Michalaki, V., Syrigos, K., Charles, P., Waxman, J., Serum levels of IL-6 and TNF-α correlate with clinicopathological features and patient survival in patients with prostate cancer (2004) British Journal of Cancer, 90 (12), pp. 2312-2316; 
Dirat, B., Bochet, L., Dabek, M., Daviaud, D., Dauvillier, S., Majed, B., Wang, Y.Y., Muller, C., Cancerassociated adipocytes exhibit an activated phenotype and contribute to breast cancer invasion (2011) Cancer Res, 71, pp. 2455-2465; 
Culig, Z., New insights into the role of interleukin-6 in human prostate cancer (2009) J Urol, 182, pp. 1255-1256; 
Chun, J.Y., Nadiminty, N., Dutt, S., Lou, W., Yang, J.C., Kung, H.J., Evans, C.P., Gao, A.C., Interleukin-6 regulates androgen synthesis in prostate cancer cells (2009) Clin Cancer Res, 15, pp. 4815-4822; 
Weber, G.F., Lett, G.S., Haubein, N.C., Osteopontin is a marker for cancer aggressiveness and patient survival (2010) Br J Cancer, 103, pp. 861-869; 
Thalmann, G.N., Sikes, R.A., Devoll, R.E., Kiefer, J.A., Markwalder, R., Klima, I., Farach-Carson, C.M., Chung, L.W.K., Osteopontin: Possible role in prostate cancer progression (1999) Clinical Cancer Research, 5 (8), pp. 2271-2277; 
McAllister, S.S., Gifford, A.M., Greiner, A.L., Kelleher, S.P., Saelzler, M.P., Ince, T.A., Reinhardt, F., Weinberg, R.A., Systemic Endocrine Instigation of Indolent Tumor Growth Requires Osteopontin (2008) Cell, 133 (6), pp. 994-1005. , DOI 10.1016/j.cell.2008.04.045, PII S0092867408006211; 
Balkwill, F., TNF-alpha in promotion and progression of cancer (2006) Cancer Metastasis Rev, 25, pp. 409-416; 
Yan, B., Wang, H., Rabbani, Z.N., Zhao, Y., Li, W., Yuan, Y., Li, F., Li, C.-Y., Tumor necrosis factor-α is a potent endogenous mutagen that promotes cellular transformation (2006) Cancer Research, 66 (24), pp. 11565-11570. , DOI 10.1158/0008-5472.CAN-06-2540; 
Bates, R.C., Mercurio, A.M., Tumor necrosis factor-α stimulates the epithelial-tomesenchymal transition of human colonic organoids (2003) Molecular Biology of the Cell, 14 (5), pp. 1790-1800. , DOI 10.1091/mbc.E02-09-0583; 
Yamauchi, T., Kamon, J., Waki, H., Terauchi, Y., Kubota, N., Hara, K., Mori, Y., Kadowaki, T., The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity (2001) Nature Medicine, 7 (8), pp. 941-946. , DOI 10.1038/90984; 
Li, H., Stampfer, M.J., Mucci, L., Rifai, N., Qiu, W., Kurth, T., Ma, J., A 25-year prospective study of plasma adiponectin and leptin concentrations and prostate cancer risk and survival (2010) Clin Chem, 56, pp. 34-43; 
Ribeiro, R., Lopes, C., Medeiros, R., The link between obesity and prostate cancer: The leptin pathway and therapeutic perspectives (2006) Prostate Cancer and Prostatic Diseases, 9 (1), pp. 19-24. , DOI 10.1038/sj.pcan.4500844, PII 4500844; 
Vaday, G.G., Peehl, D.M., Kadam, P.A., Lawrence, D.M., Expression of CCL5 (RANTES) and CCR5 in prostate cancer (2006) Prostate, 66 (2), pp. 124-134. , DOI 10.1002/pros.20306; 
Kessenbrock, K., Plaks, V., Werb, Z., Matrix metalloproteinases: Regulators of the tumor microenvironment (2010) Cell, 141, pp. 52-67; 
Bouloumie, A., Sengenes, C., Portolan, G., Galitzky, J., Lafontan, M., Adipocyte Produces Matrix Metalloproteinases 2 and 9 Involvement in Adipose Differentiation (2001) Diabetes, 50 (9), pp. 2080-2086; 
Bergers, G., Brekken, R., McMahon, G., Vu, T.H., Itoh, T., Tamaki, K., Tanzawa, K., Hanahan, D., Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis (2000) Nat Cell Biol, 2, pp. 737-744; 
Lin, C.Y., Tsai, P.H., Kandaswami, C.C., Lee, P.P., Huang, C.J., Hwang, J.J., Lee, M.T., Matrix metalloproteinase-9 cooperates with transcription factor Snail to induce epithelial-mesenchymal transition (2011) Cancer Sci, 102, pp. 815-827; 
Chakrabarti, S., Patel, K.D., Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology (2005) Experimental Lung Research, 31 (6), pp. 599-621. , DOI 10.1080/019021490944232; 
Sehgal, G., Hua, J., Bernhard, E.J., Sehgal, I., Thompson, T.C., Muschel, R.J., Requirement for matrix metalloproteinase-9 (Gelatinase B) expression in metastasis by murine prostate carcinoma (1998) American Journal of Pathology, 152 (2), pp. 591-596; 
Johnson, T.R., Koul, S., Kumar, B., Khandrika, L., Venezia, S., Maroni, P.D., Meacham, R.B., Koul, H.K., Loss of PDEF, a prostatederived Ets factor is associated with aggressive phenotype of prostate cancer: Regulation of MMP 9 by PDEF (2010) Mol Cancer, 9, p. 148; 
Trudel, D., Fradet, Y., Meyer, F., Tetu, B., Matrix metalloproteinase 9 is associated with Gleason score in prostate cancer but not with prognosis (2010) Hum Pathol, 41, pp. 1694-1701; 
Zhang, L., Shi, J., Feng, J., Klocker, H., Lee, C., Zhang, J., Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer (2004) Prostate Cancer and Prostatic Diseases, 7 (4), pp. 327-332. , DOI 10.1038/sj.pcan.4500750, PII 4500750; 
Zhong, W.D., Han, Z.D., He, H.C., Bi, X.C., Dai, Q.S., Zhu, G., Ye, Y.K., Chen, Z.N., CD147, MMP-1, MMP-2 and MMP-9 protein expression as significant prognostic factors in human prostate cancer (2008) Oncology, 75, pp. 230-236; 
Sauer, C.G., Kappeler, A., Spath, M., Kaden, J.J., Michel, M.S., Mayer, D., Bleyl, U., Grobholz, R., Expression and activity of matrix metalloproteinases-2 and -9 in serum, core needle biopsies and tissue specimens of prostate cancer patients (2004) Virchows Archiv, 444 (6), pp. 518-526; 
Opdenakker, G., Masure, S., Grillet, B., Van Damme, J., Cytokine-mediated regulation of human leukocyte gelatinases and role in arthritis (1991) Lymphokine Cytokine Res, 10, pp. 317-324; 
Unal, R., Yao-Borengasser, A., Varma, V., Rasouli, N., Labbate, C., Kern, P.A., Ranganathan, G., Matrix metalloproteinase-9 is increased in obese subjects and decreases in response to pioglitazone (2010) J Clin Endocrinol Metab, 95, pp. 2993-3001; 
Catalan, V., Gomez-Ambrosi, J., Rodriguez, A., Ramirez, B., Silva, C., Rotellar, F., Gil, M.J., Fr{\"u}hbeck, G., Increased adipose tissue expression of lipocalin-2 in obesity is related to inflammation and matrix metalloproteinase-2 and metalloproteinase-9 activities in humans (2009) J Mol Med (Berl), 87, pp. 803-813; 
Villarroya, J., Giralt, M., Villarroya, F., Mitochondrial DNA: An up-and-coming actor in white adipose tissue pathophysiology (2009) Obesity (Silver Spring), 17, pp. 1814-1820; 
Robin, E.D., Wong, R., Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells (1988) J Cell Physiol, 136, pp. 507-513; 
Kaaman, M., Sparks, L.M., Van Harmelen, V., Smith, S.R., Sjolin, E., Dahlman, I., Arner, P., Strong association between mitochondrial DNA copy number and lipogenesis in human white adipose tissue (2007) Diabetologia, 50 (12), pp. 2526-2533. , DOI 10.1007/s00125-007-0818-6; 
Yamauchi, T., Kamon, J., Waki, H., Murakami, K., Motojima, K., Komeda, K., Ide, T., Kadowaki, T., The mechanisms by which both heterozygous peroxisome proliferator- activated receptor gamma (PPARgamma) deficiency and PPARgamma agonist improve insulin resistance (2001) J Biol Chem, 276, pp. 41245-41254; 
Wilson-Fritch, L., Burkart, A., Bell, G., Mendelson, K., Leszyk, J., Nicoloro, S., Czech, M., Corvera, S., Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone (2003) Molecular and Cellular Biology, 23 (3), pp. 1085-1094. , DOI 10.1128/MCB.23.3.1085-1094.2003; 
Ducluzeau, P.H., Priou, M., Weitheimer, M., Flamment, M., Duluc, L., Iacobazi, F., Soleti, R., Malthi{\`e}ry, Y., Dynamic regulation of mitochondrial network and oxidative functions during 3T3-L1 fat cell differentiation (2011) J Physiol Biochem, 67, pp. 285-296; 
Bogacka, I., Ukropcova, B., McNeil, M., Gimble, J.M., Smith, S.R., Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro (2005) Journal of Clinical Endocrinology and Metabolism, 90 (12), pp. 6650-6656. , http://jcem.endojournals.org/cgi/reprint/90/12/6650, DOI 10.1210/jc.2005-1024; 
Toda, S., Uchihashi, K., Aoki, S., Sonoda, E., Yamasaki, F., Piao, M., Ootani, A., Sugihara, H., Adipose tissueorganotypic culture system as a promising model for studying adipose tissue biology and regeneration (2009) Organogenesis, 5, pp. 50-56; 
Tuxhorn, J.A., Ayala, G.E., Rowley, D.R., Reactive stroma in prostate cancer progression (2001) Journal of Urology, 166 (6), pp. 2472-2483
},
	month = {2012///},
	pages = {233 - 240},
	abstract = {Background/Aims: The microenvironment produces important factors that are crucial to prostate cancer (PCa) progression. However, the extent to which the cancer cells stimulate periprostatic adipose tissue (PPAT) to produce these proteins is largely unknown. Our purpose was to determine whether PCa cell-derived factors influence PPAT metabolic activity. Methods: Primary cultures of human PPAT samples from PCa patients (adipose tissue organotypic explants and primary stromal vascular fraction, SVF) were stimulated with conditioned medium (CM) collected from prostate carcinoma (PC3) cells. Cultures without CM were used as control. We used multiplex analysis and ELISA for protein quantification, qPCR to determine mitochondrial DNA (mtDNA) copy number and zymography for matrix metalloproteinase activity, in order to evaluate the response of adipose tissue explants and SVFs to PC3 CM. Results: Stimulation of PPAT explants with PCa PC3 CM induced adipokines associated with cancer progression (osteopontin, tumoral necrosis factor alpha and interleukin-6) and reduced the expression of the protective adipokine adiponectin. Notably, osteopontin protein expression was 13-fold upregulated. Matrix metalloproteinase 9 activity and mitochondrial DNA copy number were higher after stimulation with cancer CM. Stromovascular cells from PPAT in culture were not influenced by tumor-derived factors. Conclusion: The modulation of adipokine expression by tumor CM indicates the pervasive extent to which tumor cells command PPAT to produce factors favorable to their aggressiveness. {\textcopyright} 2012 S. Karger AG, Basel.},
	keywords = {Adipokines, adiponectin, adipose tissue, adult, article, cancer cell, cancer growth, Cells, Cultured, clinical article, controlled study, Culture Media, Conditioned, culture medium, Cytokines, DNA, Mitochondrial, enzyme activity, enzyme linked immunosorbent assay, explant, gelatinase B, human, human cell, human tissue, Humans, interleukin 6, Interleukin-6, male, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Middle Aged, mitochondrial DNA, Osteopontin, Periprostatic adipose tissue, priority journal, Prostate cancer, Prostatic Neoplasms, protein expression, protein induction, protein secretion, quantitative analysis, stroma cell, tissue metabolism, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha},
	isbn = {10158987 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859829669\&partnerID=40\&md5=a76be7e5c0037e77140c1fc9aca48a5a},
	author = {Ribeiro, R.J.T. and Monteiro, C.P.D. and Cunha, V.F.P.M. and Azevedo, A.S.M. and Oliveira, M.J. and Monteiro, R. and Fraga, A.M. and Pr{\'\i}ncipe, P. and Lobato, C. and Lobo, F. and Morais, A. and Silva, V. and Sanches-Magalh{\~a}es, J. and Oliveira, J. and Guimar{\~a}es, J.T. and Lopes, C.M.S. and Medeiros, R.M.}
}
