



@article {2212,
	title = {Synthesis of an O-alkynyl-chitosan and its chemoselective conjugation with a PEG-like amino-azide through click chemistry},
	journal = {Carbohydrate PolymersCarbohydr Polym},
	volume = {87},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: CAPODdoi: 10.1016/j.carbpol.2011.07.043Language of Original Document: EnglishCorrespondence Address: Gomes, P.; CIQ-UP, Departamento de Qu{\'\i}mica e Bioqu{\'\i}mica, Universidade Do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal; email: pgomes@fc.up.ptReferences: Amaral, I.F., Granja, P., Barbosa, M.A., Chemical modification of chitosan by phosphorylation: An XPS, FT-IR and SEM study (2005) Journal of Biomaterials Science, Polymer Edition, 16, pp. 1575-1593;Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., Rat bone marrow stromal cell osteogenic differentiation and fibronectin adsorption on chitosan membranes: The effect of the degree of acetylation (2005) Journal of Biomedical Materials Research, 75 A, pp. 387-397; 
Anitha, A., Deepa, N., Chennazhi, K.P., Nair, S.V., Tamura, H., Jayakumar, R., Development of muchoadhesive, thiolated chitosan nanoparticles for biomedical applications (2011) Carbohydrate Polymers, 83, pp. 66-73; 
Batista, M.K.S., Gallem{\'\i}, M., Adeva, A., Gomes, C.A.R., Gomes, P., Facile regioselective synthesis of a novel chitosan-pexiganan conjugate with potential interest for the treatment of infected skin lesions (2009) Synthetic Communications, 39, pp. 1228-1240; 
Batista, M.K.S., Pinto, L.F., Gomes, C.A.R., Gomes, P., Novel highly-soluble peptide-chitosan polymers: Synthesis and spectral characterization (2006) Carbohydrate Polymers, 64, pp. 299-305; 
Beamson, G., Briggs, D., (1992) High Resolution XPS of Organic Polymers: The Scientia ESCA300 Database, , John Wiley \& Sons New York (USA); 
Bennett, A.E., Rienstra, C.M., Auger, M., Lakshmi, K.V., Griffin, R.G., Heteronuclear decoupling in rotating solids (1995) The Journal of Chemical Physics, 103, pp. 6951-6958; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57, pp. 35-52; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Peppas, N.A., Gurny, R., Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57, pp. 19-34; 
Bhatnagar, A., Sillanp{\"a}{\"a}, M., Applications of chitin- and chitosan-derivatives for the detoxification of water and wastewater: A short review (2009) Advances in Colloid and Interface Science, 152, pp. 26-38; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arg{\"u}elles-Monal, W., Desbrires, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
Cabrera, J.C., Van Cutsem, P., Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan (2005) Biochemical Engineering Journal, 25, pp. 165-172; 
Chinchilla, R., N{\'a}jera, C., The Sonogashira reaction: A booming methodology in synthetic organic chemistry (2007) Chemical Reviews, 107, pp. 874-922; 
Chirachanchai, S., Lertworasirikul, A., Tachaboonyakiat, W., Carbaryl insecticide conjugation onto chitosan via iodochitosan and chitosan carbonyl imidazolide precursors (2001) Carbohydrate Polymers, 46, pp. 19-27; 
Crompton, K.E., Goud, J.D., Bellamkonda, R.V., Gengenbach, T.R., Finkelstein, D.I., Horne, M.K., Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering (2007) Biomaterials, 28, pp. 441-449; 
Dai, T., Tegos, G.P., Burkatovskaya, M., Castano, A.P., Hamblin, M.R., Chitosan acetate bandage as a topical antimicrobial dressing for infected burns (2009) Antimicrobial Agents and Chemotherapy, 53, pp. 393-400; 
Elkholy, S.S., Khalil, K.D., Elsabee, M.Z., Grafting of acryloyl cyanoacetohydrazide onto chitosan (2011) Journal of Polymer Research, 18, pp. 459-467; 
Gomes, P., Gomes, C.A.R., Batista, M.K.S., Pinto, L.F., Silva, P.A.P., Synthesis, structural characterization and properties of water-soluble N-(γ-propanoyl-amino acid)-chitosans (2008) Carbohydrate Polymers, 71, pp. 54-65; 
Holappa, J., Hj{\'a}lmarsd{\'o}ttir, M., M{\'a}sson, M., R{\'u}narsson, {\"O}., Asplund, T., Soininen, P., Antimicrobial activity of chitosan N-betainates (2006) Carbohydrate Polymers, 65, pp. 114-118; 
Holappa, J., Nevalainen, T., Soininen, P., M{\'a}sson, M., J{\"a}rvinen, T., Synthesis of novel quaternary chitosan derivatives via N-chloroacyl-6-O-triphenylmethylchitosans (2006) Biomacromolecules, 7, pp. 407-410; 
Iqbal, J., Sarti, F., Perera, G., Bernkop-Schn{\"u}rch, A., Development and in vivo evaluation of an oral drug delivery system for paclitaxel (2011) Biomaterials, 32, pp. 170-175; 
Kolb, H.C., Finn, M.G., Sharpless, K.B., Click Chemistry: Diverse chemical function from a few good reactions (2001) Angewandte Chemie International Edition, 40, pp. 2004-2021; 
Krishnakumar, V., John Xavier, R., FT Raman and FT-IR spectral studies of 3-mercapto-1,2,4-triazole (2004) Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 60, pp. 709-714; 
Kulbokaite, R., Ciuta, G., Netopilik, M., Makuska, R., N-PEGylation of chitosan via click reactions (2009) Reactive and Functional Polymers., 69, pp. 771-778; 
Kurita, K., Ikeda, H., Yoshida, Y., Shimojoh, M., Harata, M., Chemoselective protection of the amino groups of chitosan by controlled phthaloylation: Facile preparation of a precursor useful for chemical modifications (2002) Biomacromolecules, 3, pp. 1-4; 
Kurita, K., Yoshida, Y., Umemura, T., Finely selective protections and deprotections of multifunctional chitin and chitosan to synthesize key intermediates for regioselective chemical modifications (2010) Carbohydrate Polymers, 81, pp. 434-440; 
Lahann, J., (2009) Click Chemistry for Biotechnology and Materials Science, , John Wiley \& Sons Chichester (UK); 
Lallana, E., Fern{\'a}ndez-Meg{\'\i}a, E., Riguera, R., Surpassing the use of copper in the click functionalization of polymeric nanostructures: A strain promoted approach (2009) Journal of the American Chemical Society., 131, pp. 5748-5750; 
Li, X., Yuan, W., Gu, S., Re, J., Synthesis and self-assembly of tunable thermosensitive chitosan amphiphilic copolymers by click chemistry (2010) Materials Letters, 64, pp. 2663-2666; 
Maku{\v s}ka, R., Gorochovceva, N., Regioselective grafting of poly(ethylene glycol) onto chitosan through C-6 position of glucosamine units (2006) Carbohydrate Polymers, 64, pp. 319-327; 
Meldal, M., Tom{\o}e, C.W., Cu-catalyzed azide-alkyne cycloaddition (2008) Chemical Reviews, 108, pp. 2952-3015; 
Moln{\'a}r, E., Barbu, E., Lien, C.-F., G{\'o}recki, D.C., Tsibouklis, J., Toward drug delivery into the brain: Synthesis, characterization, and preliminary in vitro assessment of alkylglyceryl-functionalized chitosan nanoparticles (2010) Biomacromolecules, 11, pp. 2880-2889; 
Montalbetti, C.A.G.N., Falque, V., Amide bond formation and peptide coupling (2005) Tetrahedron, 61, pp. 10827-10852; 
Muzzarelli, R.A.A., Carboxymethylated chitins and chitosans (1988) Carbohydrate Polymers, 8, pp. 1-21; 
Muzzarelli, R.A.A., Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone (2009) Carbohydrate Polymers, 76, pp. 167-182; 
Muzarelli, R.A.A., Chitosan composites with inorganics, morphogenetic proteins and stem cells, for bone regeneration (2011) Carbohydrate Polymers, 83, pp. 1433-1445; 
Oh, J.K., Lee, D.I., Park, J.M., Biopolymer-based microgels/nanogels for drug delivery applications (2009) Progress in Polymer Science, 34, pp. 1261-1282; 
{\"O}nal, S., Telefoncu, A., Comparison of chitin and amberlite IRA-938 for a-galactosidase immobilization (2003) Artifical Cells, Blood Substitutes, and Biotechnology, 31, pp. 19-33; 
{\"O}zba-Turan, S., Akbua, J., Sezer, A.D., Topical application of antisense oligonucleotide-loaded chitosan nanoparticles to rats (2010) Oligonucleotides, 20, pp. 147-153; 
Park, S.O., Kwon, O.S., Choi, C.W., Kim, C.-J., Glucosamine-mediated detoxification of p-benzoquinone and its removal by chitosan (2000) Biotechnology Letters, 22, pp. 21-24; 
Prabuh, K., Sundaramoorthi, V., Selvaraju, C.K., Tamilselvi, K.K.N., Topical immunization and the use of chitosan as carrier (2011) International Journal of Research in Ayurveda and Pharmacy, 2, pp. 90-94; 
Shen, X., Ji, Y., Yang, Q., Zheng, X., Preparation, characterization, and rheological properties of dibutyrylchitin (2007) Journal of Macromolecular Science B, 49, pp. 250-258; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies Tables and Charts, , 3rd edition John Wiley \& Sons, Ltd New York (USA); 
R{\'u}narsson, {\"O}., Holappa, J., J{\'o}nsd{\'o}ttir, S., Steinsson, H., M{\'a}sson, M., N-selective {\textquoteright}one-pot{\textquoteright} synthesis of highly substituted trimethyl chitosan (TMC) (2008) Carbohydrate Polymers, 74, pp. 740-744; 
R{\'u}narsson, {\"O}., Holappa, J., Malainer, C., Steinsson, H., Hj{\'a}lmarsd{\'o}ttir, M., Nevalainen, T., Antibacterial activity of N-quaternary chitosan derivatives: Synthesis, characterization and structure activity relationship (SAR) investigations (2010) European Polymer Journal, 46, pp. 1251-1267; 
Wan, A., Gao, Q., Li, H., Effects of molecular weight and degree of acetylation on the release of nitric oxide from chitosan-nitric oxide adducts (2010) Journal of Applied Polymer Science, 117, pp. 2183-2188; 
Yuan, W., Li, X., Gu, S., Cao, A., Ren, J., Amphiphilic chitosan graft copolymer via combination of ROP, ATRP and click chemistry: Synthesis, self-assembly, thermosensitivity, fluorescence, and controlled drug release (2011) Polymer, 52, pp. 658-666; 
Yuan, W., Zhao, Z., Gu, S., Ren, J., Synthesis, characterization, and properties of amphiphilic chitosan copolymers with mixed side chains by click chemistry (2010) Journal of Polymer Science A, 48, pp. 3476-3486; 
Yuan, W., Zhao, Z., Gu, S., Ren, T., Ren, J., Synthesis and self-assembly of pH-responsive chitosan graft copolymer by the combination of atom transfer radical polymerization and click chemistry (2011) Materials Letters, 65, pp. 793-796; 
Zhang, J., Li, C., Xue, Z.-Y., Cheng, H.-W., Huang, F.-W., Zhuo, R.-X., Fabrication of lactobionic-loaded chitosan microcapsules as potential drug carriers targeting the liver (2011) Acta Biomaterialia, 7, pp. 1665-1673
},
	month = {2012///},
	pages = {240 - 249},
	abstract = {N-Phthaloyl-chitosan O-prop-2-ynyl carbamate was prepared as a biopolymer amenable to undergo chemoselective conjugation by azide-alkyne coupling, while allowing upturn of chitosan{\textquoteright}s amines after dephthaloylation. N-phthaloylchitosan was prepared according to previously described methods and, due to its low solubility in current organic media, subsequent modifications were run in heterogeneous conditions. Activation of hydroxyls with carbonyl-1,1'- diimidazole and coupling to propargylamine yielded N-phthaloyl-chitosan O-prop-2-ynyl carbamate, then coupled to a model PEG-like azide by azide-alkyne coupling, giving the expected triazolyl conjugate. N-Dephthaloylation allowed recovery of the free amines, responsible for chitosan{\textquoteright}s bioadhesion and tissue-regeneration properties. The structures of all polymers were confirmed by Fourier-transformed infra-red (FT-IR) and X-ray photoelectron (XPS) spectroscopies, as well as by solid-state nuclear magnetic resonance (SSNMR). All chitosan derivatives were poorly soluble in both aqueous and organic media, which makes them suitable for topical applications or for removal of toxic substances from either the gastric intestinal tract or environmental sources. {\textcopyright} 2011 Elsevier Ltd. All Rights Reserved.},
	keywords = {1 ,3-Dipolarcycloaddition, Acetylene, Amines, Azide-alkyne coupling, Bio-adhesion, Bioadhesion, Biomaterials, chitosan, click chemistry, Cycloaddition, FTIR, Huisgen{\textquoteright}s 1,3-dipolar cycloaddition, Nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy, Photoelectron spectroscopy, Resonance, Size exclusion chromatography, Size-exclusion chromatography, Solid state NMR, Solid-state NMR, Tissue, X ray photoelectron spectroscopy, XPS},
	isbn = {01448617 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80054771159\&partnerID=40\&md5=3760f41563573759f0ce676fe813d6c6},
	author = {Oliveira, J.R. and Martins, M.C.L. and Mafra, L. and Gomes, P.}
}
@article {2260,
	title = {Analysis of heart rate variability in a rat model of induced pulmonary hypertension},
	journal = {Medical Engineering and PhysicsMed. Eng. Phys.},
	volume = {32},
	year = {2010},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: MEPHEdoi: 10.1016/j.medengphy.2010.04.018PubMed ID: 20547091Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, H.; Departamento de Geoci{\^e}ncias, Ambiente e Ordenamento do Territ{\'o}rio, Faculdade de Ci{\^e}ncias, Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal; email: hernani.goncalves@fc.up.ptChemicals/CAS: monocrotaline, 315-22-0, 8051-27-2; Ketamine, 6740-88-1; Monocrotaline, 315-22-0References: (2005), ACOG, American College of Obstetricians and Gynecologists. ACOG Practice Bulletin. Clinical Management Guidelines for Obstetrician-Gynecologists, Number 70, December 2005. Obstet Gynecol 106(6):1453-60Akhavein, F., St.-Michel, E.J., Seifert, E., Rohlicek, C.V., Decreased left ventricular function, myocarditis, and coronary arteriolar medial thickening following monocrotaline administration in adult rats (2007) J Appl Physiol, 103 (1), pp. 287-295;Beckers, F., Verheyden, B., Aubert, A.E., Aging and nonlinear heart rate control in a healthy population (2006) Am J Physiol Heart Circ Physiol, 290 (6), pp. H2560-H2570; 
Bernardes, J., Costa-Pereira, A., A more objective fetal heart rate baseline estimation (1996) Br J Obstet Gynaecol, 103, pp. 714-715; 
Bernardes, J., Gon{\c c}alves, H., Ayres-de-Campos, D., Rocha, A.P., Linear and complex heart rate dynamics vary with sex in relation to fetal behavioural states (2008) Early Hum Dev, 84, pp. 433-439; 
Bruijnzeel, A.W., Stam, R., Croiset, G., Wiegant, G.M., Long-term sensitization of cardiovascular stress responses after a single stressful experience (2001) Physiol Behav, 73 (1-2), pp. 81-86; 
Cerutti, C., Gustin, M.P., Paultre, C.Z., Lo, M., Julien, C., Vincent, M., Autonomic nervous-systempulmonary-edema and cardiovascular variability in rats-a spectral-analysis approach (1991) Am J Physiol, 261 (84), pp. H1292-H1299; 
Court, D.J., Parer, J.T., Nathanielsz, P.W., Parer, J.T., (1984) Experimental studies of fetal asphyxia and fetal heart rate interpretation Research in Perinatal Medicine, pp. 113-169. , Perinatology Press, New York; 
Dudewicz, E., Mishra, S., (1988) Modern mathematical statistics, , Jonh Wiley and Sons, Inc; 
Falcao-Pires, I., Gon{\c c}alves, N., Henriques-Coelho, T., Roncon-Albuquerque, R., Moreira-Gon{\c c}alves, D., Leite-Moreira, A.F., Administration of Apelin modulates myocardial. expression of Apelin-APJ and improves right ventricular performance in MCT-induced pulmonary hypertension (2008) Circulation, 118, pp. S285; 
Gon{\c c}alves, H., Rocha, A.P., Ayres-de-Campos, D., Bernardes, J., Internal versus external intrapartum foetal heart rate monitoring: the effect on linear and nonlinear parameters (2006) Physiol Meas, 27 (3), pp. 307-319; 
Gon{\c c}alves, H., Rocha, A.P., Ayres-de-Campos, D., Bernardes, J., Linear and nonlinear fetal heart rate analysis of normal and acidemic fetuses in the minutes preceding delivery (2006) Med Biol Eng Comput, 44 (10), pp. 847-855; 
Gon{\c c}alves, H., Bernardes, J., Rocha, A.P., Ayres-de-Campos, D., Linear and nonlinear analysis of heart rate patterns associated with fetal behavioral states in the antepartum period (2007) Early Hum Dev, 83, pp. 585-591; 
Gon{\c c}alves, H., Henriques-Coelho, T., Bernardes, J., Rocha, A.P., Nogueira, A., Leite-Moreira, A., Linear and nonlinear heart rate analysis in a rat model of acute anoxia (2008) Physiol Meas, 29, pp. 1133-1143; 
Henriques-Coelho, T., Correia-Pinto, J., Roncon-Albuquerque, R., Baptista, M.J., Lourenco, A.P., Oliveira, S.M., Endogenous production of ghrelin and beneficial effects of its exogenous administration in monocrotaline-induced pulmonary hypertension (2004) Am J Physiol Heart Circ Physiol, 287 (6), pp. H2885-H2890; 
Henriques-Coelho, T., Oliveira, S.M., Moura, R.S., Roncon-Albuquerque, R., Neves, A.L., Santos, M., Thymulin inhibits monocrotaline-induced pulmonary hypertension modulating interleukin-6 expression and suppressing p38 pathway (2008) Endocrinology, 149 (9), pp. 4367-4373; 
Hessel, M.H.M., Steendijk, P., den Adel, B., Schutte, C.I., van der Laarse, A., Characterization of right ventricular function after monocrotaline-induced pulmonary hypertension in the intact rat (2006) Am J Physiol Heart Circ Physiol, 291 (5), pp. H2424-H2430; 
Kuwahara, M., Yayou, K., Ishii, K., Hashimoto, S., Tsubone, H., Sugano, S., Power spectral analysis of heart rate variability as a new method for assessing autonomic activity in the rat (1994) J Electrocardiol, 27 (4), pp. 333-337; 
Leineweber, K., Brandt, K., Wludyka, B., Beilfuss, A., Ponicke, K., Heinroth-Hoffmann, I., Ventricular hypertrophy plus neurohumoral activation is necessary to alter the cardiac beta-adrenoceptor system in experimental heart failure (2002) Circ Res, 91 (11), pp. 1056-1062; 
Louren{\c c}o, A.P., Roncon-Albuquerque, R., Bras-Silva, C., Faria, B., Wieland, J., Henriques-Coelho, T., Myocardial dysfunction and neurohumoral activation without remodeling in left ventricle of monocrotaline-induced pulmonary hypertensive rats (2006) Am J Physiol Heart Circ Physiol, 291 (4), pp. H1587-H1594; 
Magenes, G., Signorini, M.G., Arduini, D., Cerutti, S., Fetal heart rate variability due to vibroacoustic stimulation: linear and nonlinear contribution (2004) Methods Inf Med, 43 (1), pp. 47-51; 
Marshall, B.E., Longnecker, D.E., General anesthetics (1996) Goodman \& Gilman{\textquoteright}s. The pharmacological basis of therapeutics, , McGraw-Hill, New York, J.G. Hardman, A.G. Gilman, L.E. Limbird (Eds.); 
Martinez, W.L., Martinez, A.R., (2002) Computational statistics handbook with MATLAB, , CRC Press; 
Miller, W.C., Rice, D.L., Kreusel, R.G., Bedrossian, C.W.M., Monocrotaline model of noncardiogenic pulmonary-edema in dogs (1978) J Appl Physiol, 45 (6), pp. 962-965; 
Montenegro, N., Bernardes, J., Ayres-de-Campos, D., Matias, A., Areias, J.C., Monitoring of cardiac-extracardiac haemodynamics and automated fetal heart rate preceding intrauterine death (1996) Eur J Obstet Gynecol Reprod Biol, 64 (1), pp. 3-6; 
Moraru, L., Cimponeriu, L., Tong, S., Thakor, N., Bezerianos, A., Characterization of heart rate variability changes following asphyxia in rats (2004) Methods Inf Med, 43 (1), pp. 118-121; 
Oppenheimer, L.W., Lewinsky, R.M., Power spectral analysis of fetal heart rate (1994) Baillieres Clin Obstet Gynaecol, 8 (3), pp. 643-661; 
Parati, G., Mancia, G., Di Rienzo, M., Castiglioni, P., Taylor, J.A., Studinger, P., Point: Counterpoint: Cardiovascular variability is/is not an index of autonomic control of circulation (2006) J Appl Physiol, 101 (2), pp. 676-682; 
Pincus, S., Viscarello, R., Approximate Entropy: A regularity measure for fetal heart rate analysis (1992) Obstet Gynecol, 79, pp. 249-255; 
Pincus, S., Aproximate entropy (ApEn) as a complexity measure (1995) Chaos, 5, pp. 110-117; 
Pincus, S.M., Gevers, E.F., Robinson, I.C.A.F., VanDenBerg, G., Roelfsema, F., Hartman, M.L., Females secrete growth hormone with more process irregularity than males in both humans and rats (1996) Am J Physiol-Endocrinol Metab, 270 (1), pp. E107-E115; 
Richman, J.S., Moorman, J.R., Physiological time-series analysis using approximate entropy and sample entropy (2000) Am J Physiol Heart Circ Physiol, 278, pp. H2039-H2049; 
Sanyal, S.N., Ono, K., Derangement of autonomic nerve control in rat with right ventricular failure (2002) Pathophysiology, 8 (3), pp. 197-203; 
Signorini, M.G., Magenes, G., Cerrutti, S., Arduini, D., Linear and nonlinear parameters for the analysis of fetal heart rate signal from cardiotacographic recordings (2003) IEEE Trans Biomed Eng, 50, pp. 365-373; 
(1996), Task force, Heart rate variability - standards of measurement, physiological interpretation and clinical use Circulation 93 1043-65 Task force of the European Society of Cardiology and the North American Society of Pacing and ElectrophysiologyTaylor, J.A., Studinger, P., Cardiovascular variability is/is not an index of autonomic control of circulation (2006) J Appl Physiol, 101 (2), pp. 690-691; 
Welch, P.D., The use of Fast Fourier Transform for the estimation of power spectra: a method based on time averaging over short, modified periodograms (1967) IEEE Trans Audio Electroacous, AU-15 (2), pp. 70-73; 
Yum, M.K., Park, E.Y., Kim, C.R., Hwang, J.H., Alterations in irregular and fractal heart rate behavior in growth restricted fetuses (2001) Eur J Obstet Gynecol, 94, pp. 51-58
},
	month = {2010///},
	pages = {746 - 752},
	abstract = {Monocrotaline (MCT) is commonly used to experimentally induce pulmonary hypertension (PH), which might lead to chronic heart failure. In this study, linear and non-linear heart rate (HR) dynamics were weekly assessed in MCT-treated and non-treated Wistar rats.The HR of 10 adult Wistar rats injected with MCT (MCT group) and of 10 similar rats injected with vehicle (non-MCT group), anesthetized with Ketamine, was weekly recorded during 4 weeks. The first four segments of 1-min length of each HR recording were analysed using linear, time and frequency domains, and approximate (ApEn) and sample (SampEn) entropy indices, considering recently proposed values for the threshold parameter of ApEn and SampEn. Statistical analysis was performed using 95\% confidence intervals and statistical tests.Along the study period, an overall weekly maintenance of HR indices, or a decrease, namely in weeks 1-2, was manifest, in the MCT group, except for LF and LF/HF, in week 1, denoting a short-term increase in sympathetic activity without any other changes. On the other hand, a maintenance of HR indices, or an increase, namely on week 4, was observed in the non-MCT group, except for LF/HF, denoting a long-term increase of the overall activity of HR control systems, with a parasympathetic like dominance.Studies on long-term HR dynamics should be performed in very carefully controlled experimental settings, as significant weekly changes may occur, both among anesthetized MCT-treated and non-treated rats. {\textcopyright} 2010 IPEM.},
	keywords = {adrenergic activity, Amines, animal experiment, animal model, Animals, article, cholinergic activity, Chronic heart failures, Confidence interval, controlled study, Disease Models, Animal, Electrocardiography, entropy, Female, Frequency domain analysis, Frequency domains, Heart, heart electrophysiology, Heart rate, Heart rate variability, Heart rate variability (HRV), Heart rates, Hypertension, Pulmonary, Injections, Subcutaneous, Ketamine, Maintenance, Monocrotaline, Non-linear, nonhuman, priority journal, Pulmonary hypertension, rat, Rat model, Rats, Rats, Wistar, Statistical analysis, statistical parameters, Sympathetic activities, Threshold parameters, Time and frequency domain analysis, Time domain analysis, Time Factors, Wistar rat, Wistar rats},
	isbn = {13504533 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955279673\&partnerID=40\&md5=5fa2879d821f37e48f50a723fa1cb225},
	author = {Gon{\c c}alves, H. and Henriques-Coelho, T. and Bernardes, J. and Rocha, A.P. and Brand{\~a}o-Nogueira, A. and Leite-Moreira, A.}
}
@article {2325,
	title = {Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {88},
	year = {2009},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31849PubMed ID: 18286636Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomaterials, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: carbonyldiimidazole, 530-62-1; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; imidazole, 1467-16-9, 288-32-4; leucine, 61-90-5, 7005-03-0; lysine, 56-87-1, 6899-06-5, 70-54-2; tetraethylene glycol, 112-60-7; Ethylene Glycol, 107-21-1; Heparin, 9005-49-6; Leucine, 61-90-5; Lysine, 56-87-1; PeptidesReferences: Dailey J. Blood. Ipswich, MA, USA: Medical Consulting Group; 1998Section, V., Chronic intermittent haemodialysis and prevention of clotting in the extracorporal system (2002) Nephrol Dialysis Transplant, 17, pp. 63-71;Zhang, Y.H., Singh, V.K., Yang, V.C., Novel approach for optimizing the capacity and efficacy of a protamine filter for clinical extracorporeal heparin removal (1998) Asaio J, 44, pp. M368-M373; 
Tao, W., Deyo, D.J., Brunston, R.L., Vertrees, R.A., Zwischenberger, J.B., Extracorporeal heparin adsorption following cardiopulmonary bypass with a heparin removal device - An alternative to protamine (1998) Critical Care Med, 26, pp. 1096-1102; 
Ameer, G.A., Barabino, G., Sasisekharan, R., Harmon, W., Cooney, C.L., Langer, R., Ex vivo evaluation of a Taylor-Couette flow, immobilized heparinase I device for clinical application (1999) Proc Natl Acad Sci USA, 96, pp. 2350-2355; 
Ostuni, E., Yan, L., Whitesides, G.M., The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver (1999) Colloids Surf B-Biointerfaces, 15, pp. 3-30; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers that present oligo(ethylene glycol) groups to control the interactions of proteins with surfaces. Poly(Ethylene Glycol) (1997) ACS Symposium Series, 680, pp. 361-373; 
Cardin, A.D., Weintraub, H.J.R., Molecular modeling of proteinglycosaminoglycan interactions (1989) Arteriosclerosis, 9, pp. 21-32; 
Kricheldorf, H.R., (1987) α-Amino Acid N-Carboxyanhydrides and Related Heterocycles, , New York: Springer Verlag;; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolayers (2003) J Biomed Mater Res A, 67, pp. 158-171; 
Lee, H.J., Nedelkov, D., Corn, R.M., Surface plasmon resonance imaging measurements of antibody arrays for the multiplexed detection of low molecular weight protein biomarkers (2006) Anal Chem, 78, pp. 6504-6510; 
Roeges, N.P.G., (1994) A Guide to the Complete Interpretation of Infrared Spectra of Organic Structures, , England: Wiley;; 
Li, L.Y., Chen, S.F., Zheng, J., Ratner, B.D., Jiang, S.Y., Protein adsorption on oligo(ethylene glycol)-terminated alkanethiolate self-assembled monolayers: The molecular basis for non-fouling behavior (2005) J Phys Chem B, 109, pp. 2934-2941; 
Beamson, G., High, B.D., (1992) Resolution XPS of Organic Polymers, , Chichester: Wiley;; 
Sousa, S.R., Moradas, F.P., Saramago, B., Viseu, M.L., Barbosa, M.A., Human serum albumin adsorption on TiO2 from single protein solutions and from plasma (2004) Langmuir, 20, pp. 9745-9754; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies: Tables and Charts, , Chichester: Wiley;; 
Capila, I., Linhardt, R.J., Heparin - Protein interactions (2002) Angewandte Chemie-Int Ed, 41, pp. 391-112; 
Snell, C.R.F.G., Kinetics and thermodynamics of the alpha Helix - β Transconformation of poly(L-lysine) and L-leucine copolymers. A compensation phenomenon (1973) Biochemistry, 12, pp. 1017-1025; 
Hearn, M.T.W., 1,1'-Carbonyldiimidazole-mediated immobilization of enzymes and affinity ligands (1987) Methods Enzymol, 135, pp. 102-117; 
Stollner, D., Scheller, F.W., Warsinke, A., Activation of cellulose membranes with 1,1'-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors (2002) Anal Biochem, 304, pp. 157-165
},
	month = {2009///},
	pages = {162 - 173},
	abstract = {Systemic heparinization, used during haemodialysis to prevent blood clotting on the extracorporeal circuit, leads to a high incidence of hemorrhagic complications. The adverse reactions associated with heparin neutralization using protamine sulphate justify the development of an alternative system for blood deheparinization. The main objective of this work is to design nanostructured surfaces with the capacity to bind heparin from blood in a selective way. A heparin-binding polypeptide, composed of L-lysine and L-leucine (pKL), was synthesized and immobilized, in different concentrations, onto self-assembled monolayers (SAMs) terminated with tetra(ethylene-glycol) (EG4 SAMs). Immobilization was performed using a fixed concentration of pKL after surface activation to different degrees using a range of CDI (N,N'-carbonyldiimidazole) concentrations. Results demonstrated that the presence of pKL increases heparin adsorption to EG4-SAMs, independently of the pKL concentration and the way of immobilization (adsorption or covalent bound). Selectivity towards heparin was successfully achieved on SAMs with low concentrations of immobilized pKL (9-17\% of pKL). Surfaces were characterized using ellipsometry, contact angle measurements, Fourier transform infrared reflection absorption spectroscopy (IRAS), atomic force microscopy, and X-ray photoelectron spectroscopy. Heparin adsorption was assessed using IRAS and N-sulphonate-                        35S-heparin. Therefore, this study could give a good contribution for the design of blood deheparinization devices. {\textcopyright} 2008 Wiley Periodicals, Inc.
},
	keywords = {Absorption spectroscopy, Adsorption, Alternative systems, Amines, Amino acids, Angle measurement, article, Atomic force microscopy, Atomic forces, Atomic spectroscopy, Binding peptides, Blood, Blood clotting, Carbonyl diimidazole, Carbonyldiimidazole, Contact angle, deheparinization, device, Dialysis, Electron energy levels, ellipsometry, Ethylene, Ethylene Glycol, Fourier Transform Infrared, Fourier transforms, Glycols, Haemodialysis, heparin, heparin binding protein, Heparinization, High incidences, Humans, imidazole, immobilization, infrared spectroscopy, leucine, Low concentrations, lysine, Methods, Missile bases, Monolayers, nanomaterial, Nanostructured surfaces, Nanostructures, Peptide immobilization, Peptides, Photoelectron spectroscopy, Polysaccharides, Protein adsorption, protein binding, Renal Dialysis, Self assembled monolayers, Self-assembled monolayers, Sulphate, Surface activations, Surface functionalization, Surface Properties, surface property, tetraethylene glycol, unclassified drug, X ray photoelectron spectroscopy, X-ray photoelectron spectroscopies},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-57449118932\&partnerID=40\&md5=8f442ccbde0273f4ed4e04024a75fd37},
	author = {Martins, M.C.L. and Curtin, S.A. and Freitas, S.C. and Salgueiro, P. and Ratner, B.D. and Barbosa, M.A.}
}
@article {2313,
	title = {Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors},
	journal = {Journal of Controlled ReleaseJ. Control. Release},
	volume = {134},
	year = {2009},
	note = {Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: JCREEdoi: 10.1016/j.jconrel.2008.11.007PubMed ID: 19070635Language of Original Document: EnglishCorrespondence Address: Tom{\'a}s, H.; Centro de Qu{\'\i}mica da Madeira, Departamento de Qu{\'\i}mica, Universidade da Madeira, Campus Universitario da Penteada, 9000-390 Funchal, Portugal; email: lenat@uma.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; calcium, 14092-94-5, 7440-70-2; osteocalcin, 136461-80-8; phosphate, 14066-19-4, 14265-44-2; BMP2 protein, human; Bone Morphogenetic Protein 2; Dendrimers; PAMAM Starburst; Polyamines; beta-Galactosidase, 3.2.1.23References: Dai, J., Rabie, A.B.M., Hagg, U., Xu, R.A., Alternative gene therapy strategies for the repair of craniofacial bone defects (2004) Curr. Gene Ther., 4 (4), pp. 469-485;Gersbach, C.A., Phillips, J.E., Garcia, A.J., Genetic engineering for skeletal regenerative medicine (2007) Annu. Rev. Biomed. Eng., 9, pp. 87-119; 
Kofron, M.D., Laurencin, C.T., Orthopaedic applications of gene therapy (2005) Curr. Gene Ther., 5 (1), pp. 37-61; 
Kofron, M.D., Laurencin, C.T., Bone tissue engineering by gene delivery (2006) Adv. Drug Deliv. Rev., 58 (4), pp. 555-576; 
Luo, J., Sun, M.H., Kang, Q., Peng, Y., Jiang, W., Luu, H.H., Luo, Q., He, T.C., Gene therapy for bone regeneration (2005) Curr. Gene Ther., 5 (2), pp. 167-179; 
Partridge, K.A., Oreffo, R.O., Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies (2004) Tissue Eng., 10 (1-2), pp. 295-307; 
Turgeman, G., Aslan, H., Gazit, Z., Gazit, D., Cell-mediated gene therapy for bone formation and regeneration (2002) Curr. Opin. Mol. Ther., 4 (4), pp. 390-394; 
Chen, Y., Cheung, K.M.C., Kung, H.F., Leong, J.C.Y., Lu, W.W., Luk, K.D.K., In vivo new bone formation by direct transfer of adenoviral-mediated bone morphogenetic protein-4 gene (2002) Biochem. Biophys. Res. Commun., 298 (1), pp. 121-127; 
Lieberman, J.R., Daluiski, A., Stevenson, S., Wu, L., McAllister, P., Lee, Y.P., Kabo, J.M., Witte, O.N., The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats (1999) J. Bone Joint Surg. Am., 81 (7), pp. 905-917; 
Park, J., Ries, J., Gelse, K., Kloss, F., von der Mark, K., Wiltfang, J., Neukam, F.W., Schneider, H., Bone regeneration in critical size defects by cell-mediated BMP-2 gene transfer: a comparison of adenoviral vectors and liposomes (2003) Gene Ther., 10 (13), pp. 1089-1098; 
Riew, K.D., Wright, N.M., Cheng, S.L., Avioli, L.V., Lou, J., Induction of bone formation using a recombinant adenoviral vector carrying the human BMP-2 gene in a rabbit spinal fusion model (1998) Calcif. Tissue Int., 63 (4), pp. 357-360; 
Tsuda, H., Wada, T., Ito, Y., Uchida, H., Dehari, H., Nakamura, K., Sasaki, K., Hamada, H., Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector (2003) Mol. Ther., 7 (3), pp. 354-365; 
De Laporte, L., Cruz Rea, J., Shea, L.D., Design of modular non-viral gene therapy vectors (2006) Biomaterials, 27 (7), pp. 947-954; 
Eliyahu, H., Barenholz, Y., Domb, A.J., Polymers for DNA delivery (2005) Molecules, 10 (1), pp. 34-64; 
Roth, C.M., Sundaram, S., Engineering synthetic vectors for improved DNA delivery: insights from intracellular pathways (2004) Annu. Rev. Biomed. Eng., 6, pp. 397-426; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles (2003) Biomaterials, 24 (7), pp. 1255-1264; 
Hamm, A., Krott, N., Breibach, I., Blindt, R., Bosserhoff, A.K., Efficient transfection method for primary cells (2002) Tissue Eng., 8 (2), pp. 235-245; 
Lakshmipathy, U., Pelacho, B., Sudo, K., Linehan, J.L., Coucouvanis, E., Kaufman, D.S., Verfaillie, C.M., Efficient transfection of embryonic and adult stem cells (2004) Stem Cells, 22 (4), pp. 531-543; 
Kim, S.W., Ogawa, T., Tabata, Y., Nishimura, I., Efficacy and cytotoxicity of cationic-agent-mediated nonviral gene transfer into osteoblasts (2004) J. Biomed. Mater. Res. A, 71 (2), pp. 308-315; 
Dufes, C., Uchegbu, I.F., Schatzlein, A.G., Dendrimers in gene delivery (2005) Adv. Drug Deliv. Rev., 57 (15), pp. 2177-2202; 
Guillot-Nieckowski, M., Eisler, S., Diederich, F., Dendritic vectors for gene transfection (2007) New J. Chem., 31 (7), pp. 1111-1127; 
Svenson, S., Tomalia, D.A., Dendrimers in biomedical applications-reflections on the field (2005) Adv. Drug Deliv. Rev., 57 (15), pp. 2106-2129; 
Haensler, J., Szoka, F.C., Polyamidoamine cascade polymers mediate efficient transfection of cells in culture (1993) Bioconjug. Chem., 4 (5), pp. 372-379; 
Choi, J.S., Nam, K., Park, J., Kim, J.B., Lee, J.K., Park, J., Enhanced transfection efficiency of PAMAM dendrimer by surface modification with l-arginine (2004) J. Control. Release, 99 (3), pp. 445-456; 
Kono, K., Akiyama, H., Takahashi, T., Takagishi, T., Harada, A., Transfection activity of polyamidoamine dendrimers having hydrophobic amino acid residues in the periphery (2005) Bioconjug. Chem., 16 (1), pp. 208-214; 
KukowskaLatallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., Baker, J.R., Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers (1996) Proc. Natl. Acad. Sci. U. S. A., 93 (10), pp. 4897-4902; 
Gebhart, C.L., Kabanov, A.V., Evaluation of polyplexes as gene transfer agents (2001) J. Control. Release, 73 (2), pp. 401-416; 
Aubin, J.E., Osteoprogenitor cell frequency in rat bone marrow stromal populations: role for heterotypic cell-cell interactions in osteoblast differentiation (1999) J. Cell. Biochem., 72 (3), pp. 396-410; 
Lennon, D.P., Haynesworth, S.E., Young, R.G., Dennis, J.E., Caplan, A.I., A chemically-defined medium supports in-vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stem-cells (1995) Exp. Cell Res., 219 (1), pp. 211-222; 
Bielinska, A.U., Chen, C.L., Johnson, J., Baker, J.R., DNA complexing with polyamidoamine dendrimers: implications for transfection (1999) Bioconjug. Chem., 10 (5), pp. 843-850; 
Eichman, J.D., Bielinska, A.U., Kukowska-Latallo, J.F., Baker Jr., J.R., The use of PAMAM dendrimers in the efficient transfer of genetic material into cells (2000) Pharm. Sci. Technol. Today, 7, pp. 232-245; 
Haleem-Smith, H., Derfoul, A., Okafor, C., Tuli, R., Olsen, D., Hall, D.J., Tuan, R.S., Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro (2005) Mol. Biotechnol., 30 (1), pp. 9-19; 
Fischer, D., Li, Y.X., Ahlemeyer, B., Krieglstein, J., Kissel, T., In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis (2003) Biomaterials, 24 (7), pp. 1121-1131; 
Jevprasesphant, R., Penny, J., Jalal, R., Attwood, D., McKeown, N.B., D{\textquoteright}Emanuele, A., The influence of surface modification on the cytotoxicity of PAMAM dendrimers (2003) Int. J. Pharm., 252 (1-2), pp. 263-266; 
Wilke, M., Fortunati, E., vandenBroek, M., Hoogeveen, A.T., Scholte, B.J., Efficacy of a peptide-based gene delivery system depends on mitotic activity (1996) Gene Ther., 3 (12), pp. 1133-1142; 
Xie, Y.B., Yang, S.T., Kniss, D.A., Three-dimensional cell-scaffold constructs promote efficient gene transfection: implications for cell-based gene therapy (2001) Tissue Eng., 7 (5), pp. 585-598; 
Yamagiwa, H., Endo, N., Tokunaga, K., Hayami, T., Hatano, H., Takahashi, H.E., In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2 (2001) J. Bone Miner. Metab., 19 (1), pp. 20-28; 
Chaudhari, A., Ron, E., Rethman, M.P., Recombinant human bone morphogenetic protein-2 stimulates differentiation in primary cultures of fetal rat calvarial osteoblasts (1997) Mol. Cell. Biochem., 167 (1-2), pp. 31-39; 
Hay, E., Hott, M., Graulet, A.M., Lomri, A., Marie, P.J., Effects of bone morphogenetic protein-2 on human neonatal calvaria cell differentiation (1999) J. Cell. Biochem., 72 (1), pp. 81-93; 
Yamaguchi, A., Regulation of differentiation pathway of skeletal mesenchymal cells in cell-lines by transforming growth-factor-beta superfamily (1995) Semin. Cell Biol., 6 (3), pp. 165-173; 
Yamaguchi, A., Ishizuya, T., Kintou, N., Wada, Y., Katagiri, T., Wozney, J.M., Rosen, V., Yoshiki, S., Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3-G2/PA6 (1996) Biochem. Biophys. Res. Commun., 220 (2), pp. 366-371; 
Baltzer, A.W.A., Lattermann, C., Whalen, J.D., Wooley, P., Weiss, K., Grimm, M., Ghivizzani, S.C., Evans, C.H., Genetic enhancement of fracture repair: healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene (2000) Gene Ther., 7 (9), pp. 734-739; 
Wang, E.A., Rosen, V., Dalessandro, J.S., Bauduy, M., Cordes, P., Harada, T., Israel, D.I., Wozney, J.M., Recombinant human bone morphogenetic protein induces bone-formation (1990) Proc. Natl. Acad. Sci. U. S. A., 87 (6), pp. 2220-2224; 
Chuang, C.K., Sung, L.Y., Hwang, S.M., Lo, W.H., Chen, H.C., Hu, Y.C., Baculovirus as a new gene delivery vector for stem cell engineering and bone tissue engineering (2007) Gene Ther., 14 (19), pp. 1417-1424; 
Aslan, H., Zilberman, Y., Arbeli, V., Sheyn, D., Matan, Y., Liebergall, M., Li, J.Z., Gazit, Z., Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration (2006) Tissue Eng., 12 (4), pp. 877-889; 
Noel, D., Gazit, D., Bouquet, C., Apparailly, F., Bony, C., Plence, P., Millet, V., Jorgensen, C., Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells (2003) Stem Cells, 22 (1), pp. 74-85
},
	month = {2009///},
	pages = {141 - 148},
	abstract = {This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a β-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {alkaline phosphatase, amine, Amines, animal cell, Animals, article, beta galactosidase, beta-Galactosidase, Bone, Bone morphogenetic protein, bone morphogenetic protein 2, Bone Regeneration, Calcium, Cell culture, cell differentiation, Cell Line, cell lineage, cell survival, Cells, Cells, Cultured, controlled study, dendrimer, Dendrimers, embryo, enzyme activity, Flowcharting, gene expression, Gene transfer, Gene Transfer Techniques, gene vector, Genes, Reporter, genetic transfection, human, human cell, Humans, in vitro study, Kidney, male, Mesenchymal stem cell, Mesenchymal Stem Cells, nonhuman, Nonviral gene delivery, nonviral gene delivery system, Nucleic acids, osteoblast, osteocalcin, Osteogenesis, PAMAM dendrimers, phosphate, plasmid, Plasmids, polyamidoamine, Polyamines, priority journal, protein secretion, Proteins, qualitative analysis, quantitative analysis, rat, Rats, Rats, Wistar, reporter gene, staining},
	isbn = {01683659 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-60649096454\&partnerID=40\&md5=90df49394a581738b41bf29bad341ae8},
	author = {Santos, J.L. and Oramas, E. and P{\^e}go, A.P. and Granja, P.L. and Tom{\'a}s, H.}
}
